LOCUS       DAVLTM010000001        59543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLTM010000001 DAVLTM010000000
VERSION     DAVLTM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.001"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            120..707
                     /locus_tag="VYN16_000001"
     CDS             120..707
                     /locus_tag="VYN16_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402603.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(794..997)
                     /locus_tag="VYN16_000002"
     CDS             complement(794..997)
                     /locus_tag="VYN16_000002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402604.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(1294..1935)
                     /locus_tag="VYN16_000003"
     CDS             complement(1294..1935)
                     /locus_tag="VYN16_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1402605.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFAVGTLIVGIGLSLGGTT
                     GYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF"
     gene            complement(2003..3829)
                     /gene="glpO"
                     /locus_tag="VYN16_000004"
     CDS             complement(2003..3829)
                     /gene="glpO"
                     /locus_tag="VYN16_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1402606.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            complement(3871..5379)
                     /gene="glpK"
                     /locus_tag="VYN16_000005"
     CDS             complement(3871..5379)
                     /gene="glpK"
                     /locus_tag="VYN16_000005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1402607.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5538..6965)
                     /locus_tag="VYN16_000006"
     CDS             complement(5538..6965)
                     /locus_tag="VYN16_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1402608.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            7130..8002
                     /gene="hslO"
                     /locus_tag="VYN16_000007"
     CDS             7130..8002
                     /gene="hslO"
                     /locus_tag="VYN16_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1402609.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            7989..8969
                     /gene="dusB"
                     /locus_tag="VYN16_000008"
     CDS             7989..8969
                     /gene="dusB"
                     /locus_tag="VYN16_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1402610.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLID
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(9199..11349)
                     /gene="pspC"
                     /locus_tag="VYN16_000009"
     CDS             complement(9199..11349)
                     /gene="pspC"
                     /locus_tag="VYN16_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV1402611.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGST
                     QAATSSNMAKTEHRKAAKQVVDEYIEKMLREIQLDRRKHTQNFAFNMKLSAIKTEYLY
                     GLKEKSEAELPSEVKAKLDAAFEQFKKDTLKLGEKVAEAEKKVAEAEKKAKAQKEEDR
                     RNYPTITYKTLDLEIAESDVEVKKAELELLKEEAKTRNKDTINQAKAKVESKQAEATR
                     LKKIKTDREQAETTRLENIKTDRKKAEEEAKVKAKPKKRTKRGALGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDRRNYPTITYKTLEL
                     DIAESDVEVKKAELELVKEEAKESRDKEKIKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPTEEPENPAPAPKPEKPAEQPKAEKP
                     ADQQAEEDYARRSEEEYNRLTQQQPPKIEKPAPAPQPEQPAQPSTPKTGWKQENGMWY
                     FYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGS
                     WYYLNANGSMATGWLQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMATGWLQYN
                     GSWYYLNSNGAMVTGWLQNNGSWYYLNANGDMATDWVKDGDTWYYLEASGAMKASQWF
                     KVSDKWYYVNGSGALAVNTTVDSYRVNTNGEWVN"
     gene            complement(11428..11955)
                     /locus_tag="VYN16_000010"
     CDS             complement(11428..11955)
                     /locus_tag="VYN16_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1402612.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LVHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(12050..13390)
                     /gene="hk06"
                     /locus_tag="VYN16_000011"
     CDS             complement(12050..13390)
                     /gene="hk06"
                     /locus_tag="VYN16_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1402613.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(13387..14040)
                     /gene="rr06"
                     /locus_tag="VYN16_000012"
     CDS             complement(13387..14040)
                     /gene="rr06"
                     /locus_tag="VYN16_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1402614.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(14361..16793)
                     /locus_tag="VYN16_000013"
     CDS             complement(14361..16793)
                     /locus_tag="VYN16_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1402615.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVSAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(16795..17253)
                     /locus_tag="VYN16_000014"
     CDS             complement(16795..17253)
                     /locus_tag="VYN16_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1402616.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(17372..18100)
                     /locus_tag="VYN16_000015"
     CDS             complement(17372..18100)
                     /locus_tag="VYN16_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402617.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(18100..19107)
                     /locus_tag="VYN16_000016"
     CDS             complement(18100..19107)
                     /locus_tag="VYN16_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1402618.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(19146..19904)
                     /locus_tag="VYN16_000017"
     CDS             complement(19146..19904)
                     /locus_tag="VYN16_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1402619.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(19867..20157)
                     /locus_tag="VYN16_000018"
     CDS             complement(19867..20157)
                     /locus_tag="VYN16_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1402620.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     regulatory      complement(20230..20332)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20415..21761)
                     /gene="cbpD"
                     /locus_tag="VYN16_000019"
     CDS             complement(20415..21761)
                     /gene="cbpD"
                     /locus_tag="VYN16_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1402621.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(21850..22116)
                     /locus_tag="VYN16_000020"
     CDS             complement(21850..22116)
                     /locus_tag="VYN16_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402622.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(22118..23470)
                     /gene="dnaB"
                     /locus_tag="VYN16_000021"
     CDS             complement(22118..23470)
                     /gene="dnaB"
                     /locus_tag="VYN16_000021"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1402623.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(23514..23966)
                     /gene="rplI"
                     /locus_tag="VYN16_000022"
     CDS             complement(23514..23966)
                     /gene="rplI"
                     /locus_tag="VYN16_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1402624.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(23963..25936)
                     /locus_tag="VYN16_000023"
     CDS             complement(23963..25936)
                     /locus_tag="VYN16_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1402625.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNIQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(26072..26620)
                     /gene="raiA"
                     /locus_tag="VYN16_000024"
     CDS             complement(26072..26620)
                     /gene="raiA"
                     /locus_tag="VYN16_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1402626.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(26700..27362)
                     /locus_tag="VYN16_000025"
     CDS             complement(26700..27362)
                     /locus_tag="VYN16_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1402627.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(27359..28657)
                     /locus_tag="VYN16_000026"
     CDS             complement(27359..28657)
                     /locus_tag="VYN16_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1402628.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            28713..29348
                     /locus_tag="VYN16_000027"
     CDS             28713..29348
                     /locus_tag="VYN16_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1402629.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            29633..30559
                     /gene="cysK"
                     /locus_tag="VYN16_000028"
     CDS             29633..30559
                     /gene="cysK"
                     /locus_tag="VYN16_000028"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1402630.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVYAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDSIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEFEV"
     gene            complement(30680..31720)
                     /locus_tag="VYN16_000029"
     CDS             complement(30680..31720)
                     /locus_tag="VYN16_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1402631.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(31799..32578)
                     /gene="rpsB"
                     /locus_tag="VYN16_000030"
     CDS             complement(31799..32578)
                     /gene="rpsB"
                     /locus_tag="VYN16_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1402632.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            complement(32802..33980)
                     /locus_tag="VYN16_000031"
     CDS             complement(32802..33980)
                     /locus_tag="VYN16_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1402633.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINIVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            complement(34074..34568)
                     /gene="mreD"
                     /locus_tag="VYN16_000032"
     CDS             complement(34074..34568)
                     /gene="mreD"
                     /locus_tag="VYN16_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1402634.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            complement(34568..35386)
                     /gene="mreC"
                     /locus_tag="VYN16_000033"
     CDS             complement(34568..35386)
                     /gene="mreC"
                     /locus_tag="VYN16_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1402635.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            complement(35445..36239)
                     /locus_tag="VYN16_000034"
     CDS             complement(35445..36239)
                     /locus_tag="VYN16_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1402636.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            complement(36232..37071)
                     /locus_tag="VYN16_000035"
     CDS             complement(36232..37071)
                     /locus_tag="VYN16_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1402637.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            complement(37056..37883)
                     /locus_tag="VYN16_000036"
     CDS             complement(37056..37883)
                     /locus_tag="VYN16_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1402638.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            complement(37880..38425)
                     /gene="pgsA"
                     /locus_tag="VYN16_000037"
     CDS             complement(37880..38425)
                     /gene="pgsA"
                     /locus_tag="VYN16_000037"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1402639.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            complement(38436..39257)
                     /locus_tag="VYN16_000038"
     CDS             complement(38436..39257)
                     /locus_tag="VYN16_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1402640.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            complement(39299..40582)
                     /locus_tag="VYN16_000039"
     CDS             complement(39299..40582)
                     /locus_tag="VYN16_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1402641.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSIGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            complement(40579..41829)
                     /locus_tag="VYN16_000040"
     CDS             complement(40579..41829)
                     /locus_tag="VYN16_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1402642.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            41988..42356
                     /gene="yaaA"
                     /locus_tag="VYN16_000041"
     CDS             41988..42356
                     /gene="yaaA"
                     /locus_tag="VYN16_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1402643.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            42359..43456
                     /gene="recF"
                     /locus_tag="VYN16_000042"
     CDS             42359..43456
                     /gene="recF"
                     /locus_tag="VYN16_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1402644.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(43507..44985)
                     /gene="guaB"
                     /locus_tag="VYN16_000043"
     CDS             complement(43507..44985)
                     /gene="guaB"
                     /locus_tag="VYN16_000043"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1402645.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(45137..46162)
                     /gene="trpS"
                     /locus_tag="VYN16_000044"
     CDS             complement(45137..46162)
                     /gene="trpS"
                     /locus_tag="VYN16_000044"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1402646.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            46368..47990
                     /locus_tag="VYN16_000045"
     CDS             46368..47990
                     /locus_tag="VYN16_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1402647.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            48052..50604
                     /locus_tag="VYN16_000046"
     CDS             48052..50604
                     /locus_tag="VYN16_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1402648.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPISYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(50634..51811)
                     /locus_tag="VYN16_000047"
                     /pseudo
     CDS             complement(50634..51811)
                     /locus_tag="VYN16_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            51918..52460
                     /locus_tag="VYN16_000048"
     CDS             51918..52460
                     /locus_tag="VYN16_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1402649.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(52501..52574)
                     /locus_tag="VYN16_000049"
     tRNA            complement(52501..52574)
                     /locus_tag="VYN16_000049"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52538..52540),aa:Asn,seq:gtt)
     gene            complement(52580..52651)
                     /locus_tag="VYN16_000050"
     tRNA            complement(52580..52651)
                     /locus_tag="VYN16_000050"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52616..52618),aa:Glu,seq:ttc)
     gene            complement(52703..53455)
                     /locus_tag="VYN16_000051"
     CDS             complement(52703..53455)
                     /locus_tag="VYN16_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1402650.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(53452..54777)
                     /locus_tag="VYN16_000052"
     CDS             complement(53452..54777)
                     /locus_tag="VYN16_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1402651.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(54798..54923)
                     /gene="comC"
                     /locus_tag="VYN16_000053"
     CDS             complement(54798..54923)
                     /gene="comC"
                     /locus_tag="VYN16_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1402652.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(55088..55161)
                     /locus_tag="VYN16_000054"
     tRNA            complement(55088..55161)
                     /locus_tag="VYN16_000054"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55125..55127),aa:Arg,seq:tct)
     gene            complement(55205..55684)
                     /gene="rlmH"
                     /locus_tag="VYN16_000055"
     CDS             complement(55205..55684)
                     /gene="rlmH"
                     /locus_tag="VYN16_000055"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1402653.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            55867..57048
                     /locus_tag="VYN16_000056"
     CDS             55867..57048
                     /locus_tag="VYN16_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1402654.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            57106..57864
                     /locus_tag="VYN16_000057"
     CDS             57106..57864
                     /locus_tag="VYN16_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1402655.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            58077..59438
                     /gene="dnaA"
                     /locus_tag="VYN16_000058"
     CDS             58077..59438
                     /gene="dnaA"
                     /locus_tag="VYN16_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1402656.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT        18180 a        12754 c        10004 g        18605 t
ORIGIN      
        1 tataataaaa gttgaaaaaa atcaaaaata caaaggagtt tccgttatgt ctaagatgtt
       61 taaatgcatg tgtttgctag ttatgtctgt tttctgtatg tctagcattt ccccgtcgct
      121 tgtttgtttt tgctgatgat agcttatcgg caaactctaa agttgtatct ggtgaggctc
      181 agtttgagaa tggctcatca gtgcggttcg gagatacgca agtaaatatt ttaagcgatg
      241 aagttcttga ggttgtcaat ccagatggaa gtgtagacac tattgaacga cgtgctgacg
      301 gcgtctatat caatggggca ttttatatgg cttatcaaaa gaatgaaatc gacctaaata
      361 tatcttttag atcatacgat cctaatgttt ggaattacgt caacacaata catgggaata
      421 aacaagctaa cacattcgct aacttcatga ctggtgctgg aatcagttat atgattggta
      481 ggataggtgc tttattgggg ggtccatggg gtgcaataat tggtggagca tattttggta
      541 tacaagctta tcagtcttac ttggattcac aaagcccata tccatactac ataacaagta
      601 cttatatcca tgttgctcaa agaaaatgga agtttatcac tgaatactat agaaactcaa
      661 actatactgg gtatgtaaaa accgtaacaa cctacgtgaa tttctaaaat ataaaccctg
      721 cctagatata aaggcagggt ttatatttta gttaattgct caagtaattt gtaagcataa
      781 tacttgcgaa actctatttg ttcaagcggt tcaaaagctc ttgtttttta ccctcaagct
      841 cagctctcct ttgctcgatt tcttcacgtt tttgggcgat ttctgcttgc gcctcgggag
      901 tttccaaagt gccttgtgac ttgtgaaatt ctactagcat atcaacgatt tttgggtcta
      961 attcgttgac ataatcagga tttgaccatt tcggcacgtt gcttgcaggt gcttgctttt
     1021 tgccgccttt tttgggcgtt ttggcttgtt gttcagcttt ctgactgtag ttgacctcgt
     1081 ggaattttcg tgcttgatat gatgcgtttt attatgagaa gatttacttc attttttgct
     1141 gaaattgggc tcttgcccaa gctctatcaa aataattgtt ttctaatact caatgaaaat
     1201 caaagagcaa actaggaagc tagccgcaag ctgtacttga gtacggtaag gcgacgctga
     1261 cgtggtttga atttgatttt cgaagagtat aaactagaaa agtgagaata caagcactgc
     1321 caaggctgct ccgataacag ggcctacaac aggaatccaa gcgtaagacc agtctccgtc
     1381 tcccttgttt ggaattggca agatgctgtg catgatacga ggtccaaggt cacgagctgg
     1441 gttcaaggca taacctgttg tcccacctag tgatagaccg ataccgacaa tcaaagttcc
     1501 cactgcaaag gttccaagga tttcgctaat caagtttgat acagtatcct tgatggctgg
     1561 tccagtactg aaggttgcca ggatattgcc tgcattttct tctacctcat aatgcggttt
     1621 gaattgcaac caaaccaaaa tctgacccag catggcccct gcgaactggg ctaagatata
     1681 aggcaaaacg gaagcccaag gcaaaccacc ttttaaggcc acaccgatgg tcacagctgg
     1741 gtttaaatga gctggactga gcttgccaga tacaaatact gcaaccgcaa ctgcaatccc
     1801 ccaacccata gtaatcacaa tccaacctgc actgttgctc ttggtttttg gaagaaccac
     1861 acctgcaaca acaccatttc ctagaagaat caggattaaa gtccctaaaa attctccaaa
     1921 taattcactc atcatttttc tgtctccatt aaaaaagggg cgggcgacaa ggattgctac
     1981 cctccacctc ttttattttt tcttaatttt ttaattctgc taagtcgttt tgagcaagag
     2041 ctgcttttac atcagcacgg taagttgctt tttcttcttc tgtccagtca tagaatcgtc
     2101 ccatttcatc caaaactggc tcaacgatac tatccaagct atcacgcata aagagcatgt
     2161 gattggtacg acgaagaagg aagtcaactg ggctaagagt caactcattg cgcattgcat
     2221 agtgaagaga caaggtgtct gctaagctaa gacctggcgc ttgttccaag ctgtgagcaa
     2281 gtgcaaagac cttcggtgca tttgaaccat aaagatttgc taggtaatga gcttccttgc
     2341 tatccaaacc acgtgaaact ccaagttgcg caaaggcttc gatttctgaa tccacatttg
     2401 ctgggttcaa ttctccacct gaaacagggt aagttttaga attgatcaat ttaaagctac
     2461 ggtcaaattc tgctttgagg atgtcaacca cgcgctccat agctccttca gccatcttac
     2521 ggtagtctgt gattttacca ccagcaagag tcaaaagacc attgtcatca cggtccaagc
     2581 tagacccacg agaaactgca gatggatcca aatatttctc agatgtgcta ctttcaagct
     2641 tgctgacagc agactcaaca tcttcacgtg ttttttcttt ggagagataa gattcaacag
     2701 tcgcaatcaa gttgtcaaag ctttcatcac tgatggtacc gttatttcca ccattatagt
     2761 cagaggcact gttccctgca atcaatggac gaagacctgc ccagctgctt tcgatatcat
     2821 caatggtgat gttggattct ggaaagcggt tgttgacaat gccaagtaga taatctacat
     2881 cttcttgagt tacttttgga tgctccaaat cacctgtgta gtctgtatca gttgtaccaa
     2941 agtaagtctt gttttcacgt gggagaacaa agaccatacg accgtcaccc aaacctgtgt
     3001 caaagtaaac tggctgtgaa accttgattt tgcttgaatc cactaccaag tgaactccct
     3061 tagttgggcg catttgtgag aattgcgttc ccttattaga caaattacgt actttatcac
     3121 tccaaggacc tgttgtatta ataaccagac gggccttgat ttcaaatact tggtctgtca
     3181 agagatcacg agctacaaca cctgtaatct tgccactttc gtcaaagagg aagccttctg
     3241 ccttcacgtg gttggcaatg agggcaccgt cttggttggc acgtttgatg ttttcaatca
     3301 cgagacgcgc atcgttgtta cggaagtcaa gatacactcc acctcctacc aagccttcct
     3361 tcttcaagtt tggctggcgt tccaagactt gatccttgct caaaaccttg ttcgcagttg
     3421 gtgtgttgct cacacctgct aaaaggtcgt acaagtccat ggctacttta agacggaaga
     3481 ggctaaaggt tgctccatct tcatcgtaaa ctggtaagag cattggatct ggttttggaa
     3541 tgtgtggagc gatttgttga accactgcac gttcagaaac cgtatctgag accacctcta
     3601 cgtcaaattg tttgaggtag cgaagacctc cgtgaaccaa ttttgttgaa cgactagatg
     3661 ttccttctgc aaagtcttgc atttcaatca aaccagtctc aagaccgcta gctgccgcct
     3721 gcaaggctac accagctcct gtgattcctc caccgataat caagaggtcc agggtgcgtt
     3781 cctgcatttt tttaattgac aattcacgtg ttttctttga aaattccata tttacacact
     3841 ttctaaataa atcgctttat tctgccagta ttagtcgtct acttccgcaa agacttgagt
     3901 tgctttcaca gccttcttcc agcccttgta gagttgttcc ttgcgagatt cgttcataga
     3961 tggctcaaag agttctcctg tctcgttcaa gagtttcaac tcgtccaagt ctttccagta
     4021 ccctactgac aaacctgcta ggaaggccgc tcctagagct gttgtttcca ggtttttagc
     4081 acgtgcaata tcaatgccta aaatatccgc ctggaactgc atgaggaagt tgttcatggc
     4141 tgcaccacca tccaccttca atacttgaat ggcggtctga gtatccactt gcatggtgtc
     4201 gatgatgtca cgcacttgat aagcaataga ttgcaaagtc gccttgataa agtcttcttt
     4261 gcttgttcca cgagtcaaac caaagacgga accacgagca ttttggttcc agtatggagc
     4321 gcctagacct gtaaaggctg gaacgacata aacttcatcg ttgttgtgag aatcacgagc
     4381 gtatttttca gattctggtg aattttcaac catgcgaaga ccgtcacgaa gccactgaat
     4441 agcacttcct gcgatgaaga tagaaccttc caaggcataa taaaccttac cgttgattcc
     4501 gtaaccaatg gttgtcaaga ggttgttttc agacaactgc atctcttccc cagtattcat
     4561 gatgatgaaa gagcctgttc cataagtatt cttaaccata cctggctcaa aagccaactg
     4621 tccaaagagg gctgcttgtt ggtccccagc catacctgag attggcacct ctccaccgta
     4681 gaaatggaat ggagctgtct tgccgtagat ttcggagtta gaacgaactt ctggaagtat
     4741 agccttcgga atgttaagga tttccaaaat ctcatcatcc catttgagtt ctttaatgtt
     4801 ataaagcatg gtacgagctg catttgagta gtcagtcacg tgagccgcac cgtcagtcaa
     4861 tttccaaacc aaccaagtat cgatagtacc aaagagcaat tccccttttt ctgctcgctc
     4921 ttgagcgcct tctacatgat ccaaaatcca acgaaccttg gtagcagaga agtaagcatc
     4981 aataatcaaa ccagtctttt catggaattt ttccacataa ccttggcttt ttagttgctc
     5041 agccaaaggt gctgtctggc gtgactgcca aacgatagca ttgtagatag gaagtcctgt
     5101 tttcttatcc cagacaaccg ttgtttcacg ttggttggta atcccgattg cctcgatttg
     5161 atttggcttg acaccacttt cgatgaaagc acccgcaata actgactgaa cagagttcca
     5221 aatttcattg gcattgtgct caacccaacc tgcctgaggg aaaatctggg taaactcttt
     5281 ttgactcgag ctaacctttt cccctttttt gttgaaaatg atggcacgag aacttgtagt
     5341 tccctggtca atggccatga tgtatttttc ttgtgacatg tactgtcctc ctgataaaga
     5401 tcttgaggtg tttcctcttt acacttacta gtatacaaaa taaagcgctt tcttgtttat
     5461 caactttttt taatttttaa aaaaatgtga ggagattgtg tgaatttcac aaaaaagacc
     5521 tggattaacc aagcctttta agtgaataat aactgacgaa tccctgccaa atcttccata
     5581 tccaagtcgt tttttaaata atagactggt gtctgttcct tcttatgaat cgggttattg
     5641 gtaaccagca aatcataatg ccgactaggg tcataagctt caatggtaat aaaacgattg
     5701 tattccaaat accgtttcaa aatggctgcc atccttgaaa agacaagaaa accagatgtc
     5761 aaatccagac caatccgcat atgctgacca ccgttttcag ccatatattc gattaactgg
     5821 agccattccc agagaatctt cttatctaaa tctgtcccct gttgaaaagc aggtagagca
     5881 tgaaaaatct cctctgctgt ctctttaaaa tcatgttcca tcagtaaata tggatgacga
     5941 ttctctaact ggtacttgta gcgatcctgt aaaatatagc ccttatagag atagacttga
     6001 gcacaaagct gactgagttc ataggtgaca tggtcctctg tataatcccc caatagttcc
     6061 tccttcttca tttcttgaat caattgcgtc agtaaatctg ccaactgccc tccaaaacca
     6121 agaatatact ccatagtatg aagaggaaga atgcgatgag ataggagaaa agagaagaat
     6181 atcatcatct caccatcctc aactcctagt ggaatatgtt gcccagcaaa aaaggacgga
     6241 acctttctca gcaaacgaag ataaaagata ttgtcaaaat accctcgcat tttctcttct
     6301 atgacttgaa actgacaagc attgacccga agacgttgtt gactgatgtg agcccagaga
     6361 accaagtcca atttctgccc cacagacaaa cttgcaccgc agatttcctc taaattggca
     6421 atctcctgtt ttctctctgg tttctgcatg tgaccttccc attcctgact cgaccagacc
     6481 tttcggaaaa gacagaaata gaaatagcga atctgatgct ctggacctcg ccaacgtcca
     6541 ttttggatgg ataaatcaaa ttctgacaaa atctgattta aaccagccaa gtgacgacca
     6601 agcgtagcct cgctaatcac caattcttga gccagctgat gggctaaaaa ctgttggtgg
     6661 tagagaagat aaaccaaaat ctggtattta acagcactct ccaaaaacaa gctccgaata
     6721 tctctcccct tggtagctgc accgatagac agacgcagat tttcatcttc tgagtgaata
     6781 gtcagctcta agccactatc caaagccttg tcattgagca gggtgacata tttggttagg
     6841 gttgcttttg aaaatcctgt ttcttccatt acagccttga cggtcgtctg agactcttgt
     6901 aatagaaagg aaaggattga aaattgacca cactcggctt tctccatcaa atctcctaaa
     6961 tacatttgtt acccctttca ttttctacct taagcatagc ataaatctta caaatgctaa
     7021 aataatctgt ttctcttttt attttcatgc tgatttcctg gtccattatc ctgaaaatca
     7081 gtaaacacac ggctccccct ttgggcattt ttatgctaaa atagtagcta tggataaaat
     7141 tattaaaact atatcagaaa gcggagcctt tcgtgctttt gtccttgata gcacggaaac
     7201 cgtccgcact gctcaagaaa aacatcaaac ccaagctagc tcaactgtag cgcttggtcg
     7261 aactcttatc gccagccaga ttctcgcagc caatgaaaaa ggaaatacca aacttacagt
     7321 taaggtgttg ggctctagct ctctaggtgc tattatcacc gtcgctgata ccaaggggaa
     7381 cgtcaaaggc tatgttcaaa atcctggtgt tgacatcaaa aagactgcga ctggtgaagt
     7441 cctagtcgga ccttttgttg gaaatggtca attcctcgtt atcacagact acggtactgg
     7501 aaatccttac aactctataa ctcccctcat ctctggagaa atcggtgaag accttgcctt
     7561 ttaccttact gaaagccaac aaacgccttc agcggtcggc ctcaatgtcc ttttggacga
     7621 ggaagacaag gtcaaggttg caggtggttt cctagttcaa gtcttgccag gagccaagaa
     7681 agaagagatt gctcgctttg aaaaacgcat ccaagaaatg ccagctatct ctactcttct
     7741 cgaaagcgac gaccatatcg aagccctcct caaggctatc tacggggacg aagcctacaa
     7801 gcgtctttct gaagaagaaa tccgtttcca atgtgactgt agccatgaac gctttatgaa
     7861 cgctcttgcc agccttccaa gctcagactt acaggaaatg aaagaggaag accacggggt
     7921 agaaatcact tgtcaattct gccaaactac ttacaacttt gatgaaaagg acctggagga
     7981 actcattcgt gacaaatctt aatacacctt ttatgattgg caatgttgag attcccaatc
     8041 gtaccgtttt agcgcctatg gctggcgtga ccaactcagc ctttcgtacc atcgcaaaag
     8101 agctcggagc tggactcgtt gtaatggaaa tggtctctga caagggaatc caatacaaca
     8161 acgaaaaaac cctgcacatg cttcatatcg atgagggcga aaaccctgtc tctatccaac
     8221 tttttggtag cgatgaagac agcctagcac gcgcagcaga atttatccaa gaaaacacca
     8281 agaccgatat cgtcgatatc aacatgggct gccctgtcaa caaaatcgtg aagaacgaag
     8341 ctggtgctat gtggctcaag gatccagaca agatttactc catcatcaac aaggtccagt
     8401 ctgtccttga tatcccactt actgtcaaaa tgcgtaccgg ctgggcggac ccatctctgg
     8461 cagtagaaaa tgccctcgct gctgaagctg caggtgtttc tgccctcgcc atgcatggcc
     8521 gtacccgtga acaaatgtat actggccacg cagaccttga gaccctttac aaggttgccc
     8581 aagctctaac caagattcca ttcatcgcca acggtgatat ccgtactgtc caagaagcca
     8641 agcaacgcat cgaagaagtt ggtgctgacg cagtcatgat tggccgagct gccatgggaa
     8701 atccttacct cttcaaccaa atcaaccatt actttgaaac aggagaaatc ctacctgatt
     8761 tgacctttga agacaagatg aagatcgcct acgaacactt gaaacgattg attgacctca
     8821 aaggagaaaa cgtcgcagtt cgtgaattcc gcggcctcgc tcctcactat ctccgtggaa
     8881 catctggcgc tgccaaactc cgtggagcca tttcgcaagc tagcaccctg gcagagattg
     8941 aagccctctt gcaattggag aaggcttaat agtttaaaac ccgtaactct cttaaagagc
     9001 ctcttgaatg ccgccattct ttcctcatca aaaaaactcc gtagtctcta gcgctataaa
     9061 tccggctaga gattacagag ttatttttta atctaaactt cctacaaggg tatattatct
     9121 actgaaagga tatggtattt gtagggaata ctttaaagag ttcttacagg aagtcagtat
     9181 taactagtta tattaggttt agtttaccca ttcaccattg gtattgactc tatagctatc
     9241 tacagttgtg ttgactgcaa gggcacctga gccattgaca tagtaccatt tatctgatac
     9301 tttgaaccat tggcttgctt tcatagcacc tgatgcttca agatagtacc aggtatctcc
     9361 atctttcacc caatctgtcg ccatatcacc attagcgttt aggtagtacc atgagccatt
     9421 gttttggagc catcctgtta ccatagcacc attgctgttg aggtagtacc atgaaccatt
     9481 gtattggagc catcctgtcg ccatatcacc attagcgttg aggtagtacc atgaaccatt
     9541 gtattggaac catcctgtcg ccatatcacc attagcgttg aggtagtacc atgaaccatt
     9601 gtattggagc catcctgtcg ccattgaacc attagcgttg aggtagtacc atgaaccatt
     9661 gttttggagc catcctgttg ccattgaacc attagcgttt agatagtacc atgaaccatt
     9721 gttttggagc catcctgtcg ccatagcgcc attgctgttg aggtagtacc atgagccatt
     9781 gttttggagc catcctgtcg ccattgaacc atcagtattg tagaagtacc acataccgtt
     9841 ttcttgtttc cagcctgttt ttggagtaga tggttgtgct ggttgctctg gttgtggtgc
     9901 tggagctggt ttttcaattt ttggcggttg ctgttgagtc aagcgattat attcttcttc
     9961 tgatctacga gcatagtctt cttcagcttg ttgatcagct ggtttttctg cttttggttg
    10021 ttcagctggc ttctctggtt ttggagctgg agctggattc tcaggctctt cagttggttt
    10081 ttctggttga ggagccggcg ctggttgtgg ttgttcagct ggtttttctt taactttatc
    10141 ttcttctgct gcttttcgtt tagcttcttc ttcttctgct tttttacgat ctgtcttgat
    10201 tttttctaac cttgtagcct cagctttttt actctcaact tccgcttttg cttgcttaat
    10261 tttttccttg tctcgagatt ccttagcttc ctcttttact agttcaagct ccgctttttt
    10321 aacttccaca tcggactcag caatgtcaag ttcaagcgtt ttgtaagtaa tggttgggta
    10381 attacggcga tcttcttctt tttgatcctt ggcttttttc tcagcttctt caaccttctt
    10441 ctcagcttct gctacctttt ttcctggttt cagggatggg cttggaagag tttcttcacc
    10501 tacgctagaa tctgaagact tcgcatcatt ttctttttta tcaggtgttg ctggctctcc
    10561 aagagctcct cgttttgtcc gcttctttgg tttagcttta actttagctt cttcttctgc
    10621 ttttttacga tctgtcttga tgttttctaa ccttgtagtc tcagcttgtt cacgatctgt
    10681 cttgattttt tttaaccttg tagcctcagc ttgtttactc tcaactttcg cttttgcttg
    10741 gttaattgtg tccttgtttc gagttttagc ttcctctttc aatagttcaa gctccgcttt
    10801 tttaacttcc acatcggact cagcaatttc aaggtcaagc gttttgtaag taatggttgg
    10861 gtagttacgg cgatcttctt ctttttgagc cttggctttt ttctcagctt ctgcaacctt
    10921 cttctcagct tctgctacct tttctcctag tttcaatgta tcttttttaa actgctcaaa
    10981 agctgcgtct aactttgctt ttacttctga cggcaactca gcttccgact tctcttttaa
    11041 tccatacaaa tactccgttt taattgcgct caacttcatg ttgaaggcga aattttgggt
    11101 atgttttctt ctatctaatt gaatctccct caacattttt tctatatatt catcgacgac
    11161 ttgtttagca gctttcctat gttctgtctt tgccatatta gaagaagtgg ctgcttgggt
    11221 acttccctcg ttctctgtcg catgaaccac acttcccata acaagactgg caactactac
    11281 actagctact ccaatactaa atttacgaat tgaataatgt acttttcttt cgctttttga
    11341 tgcaaacatg tttatttcct tctatatttt ttctttaacc ttactatatc acttcaatat
    11401 gaactgtata taaatcgaat tttgtattta tctctttatg cttccatttg ggattataac
    11461 ttcatgtaat agcttttcct cttcagctat tcgtcgatac aaaataactg cataaactgg
    11521 aaaaactaaa acaaaagtca tataggcatg actcaacaag gtcaagccaa tcaactcagg
    11581 taaaatattt aaaaagtaat taggatgttt tactgtccta aacaagatat gatctacata
    11641 tttgtgatta ttgacaagca taagcttcac tgtccaaata tctcccaaca agtgaatcac
    11701 caacatcaac atcagcatag aaaaaataag caagactaaa ccaaccatgc tcataccatc
    11761 aaacattgtc ttatgcacca ttgcttcaac gaggcaagag agataaaaaa caatatgaac
    11821 caacgttaat cgttttgtat tctcaactcc aaattctttc ccgccattgc taaggattcg
    11881 tttctcattc tctatcgaac gctttaaaaa aatcaatcga ataaggaatg ttactaaaat
    11941 aatcgctaat accatattag tatcctttct tttatctata tttttatcta tataaagaat
    12001 aatacactta tgttaaccct atatgaactt taataaaaaa ctaatctgtc tacaagctaa
    12061 tcttaaattc cataccgtgt tcataaggga gaaaactata gtccatttga tagctttcta
    12121 aaatattatt cacaatgtaa agacccaaac cggaactttc atccttatct gtcacgattt
    12181 gagaatgata gaatatatca aataaatgtt ctagttcttg gtcacttaga ggcgcacatg
    12241 tatttttgat agagagatag ccatcttgct cggatatgat tacgcgccca tttttatctg
    12301 aatatttaat tgcattacta atcaggtttg tcaaaacctt atccaatgcc ctaagactca
    12361 tgacgaccct ggtagcatct gtcaattgat tttcaattgt aacacctatt gaatgagcta
    12421 attcttgata acgtgataaa atatctacta aaactggctt aacagtcaaa gtctcacgac
    12481 actctgtcca ctcttggaat ttagaagact ccagtacttc ttccaataag tggctcatct
    12541 ggtcaatctt atttatactc tttgcaatat attttggatg atctttgtaa tctccaatat
    12601 tatgctgcat attctctagg ataattctaa gactggctaa aggggttttc aactcatgtg
    12661 atgctccgcg gacaaaggaa accttttgat tttgcaattt tacaatttgc tcattacgac
    12721 tttccaattc atgaataacc ttcaacaagt gctcatacat accattaatc tgttttccaa
    12781 cttcaccaac ttcaccaact tcatctttcc tactttcatc aaaacgaata ttgtcatcca
    12841 aatcttgcat tttactagtc acttctgaaa tgtaaaaaag aggattgagc aagcgtttag
    12901 tataaaaata agaaaaaaca aaggaaaaag caattgtaac caaaaatgta tatgggagat
    12961 acaaaagcaa aatattcttt gcttctttgt agacatccac cccgtgaaca aattgtacgg
    13021 ttacctgttt accatctgct gtactaacag acatatctaa catcacgata taatttgata
    13081 gctttccatc tttaatatcc aagtcatgca caagagggag ccgcttatct acaatatctc
    13141 ttttaaggtg agcagatatg gtcaaggact ttgaataaga ctggagtaag cttggaattt
    13201 catcagatgt cttagtcttt aaatactccg taaacactct tgcgctctca ttaaactttt
    13261 ccccctccag ttgaatataa taaaaaggaa aggtcaaata aatagctata tgaatgacta
    13321 gaccaacacc acctagaatt gcaaaacttc ttaaaaaaga cttggttaat aatttaggat
    13381 tttttatcat aagctaatct tatacccaac atttttcaca gtcacgatac aatctaaagc
    13441 taattttttg cgaagagtac gaatatagac atcaacgacc ctatcacaag gtaaatcagc
    13501 tacatctttt gaaatttctt ccaatatctg ctctctactt aaaacttgat ttttatgctg
    13561 aatcaaacac tttagtacca gtaattcctt tggtttgaga tcaatttctt catttttata
    13621 atgtgctttg taagaggtaa aatccactgt tacatcctga tatcgccaaa gatcctctat
    13681 gacgtagtaa cgtctgataa gcgccttaat acgcttgaca agcagtacca aagaaaaagg
    13741 ttttgtcaca tgatcatcag catagagatc aaatacctgt gactgagtag tttcatctcc
    13801 cagagcactc aagacgatga caggaatatc gtgcttttga ttgatttctt ttaacacttc
    13861 aaaaccactc ttcctaggca tcattaaatc cagtaccatg agatggatag ggagatcttg
    13921 aaatttttcc aagacctctt gtccatcctt agccataatg acatgataac cctcttctgt
    13981 cagaaatgct gcaattcctt ctctaatcat ttcctcgtca tctgcaacta aaatgttcat
    14041 ctctctccct ttctactacc agaactccat acatctattt cctatatgct atttgaaaat
    14101 ttcctaccag cctatcatct atgataatgg aatggatttc atttatccgc tactttttgt
    14161 caacaacaac gtcaatacaa gaaaatcatt catcttgcag cttatactct tcgaaaatct
    14221 cttcaaacca cgtcagctct atctacaacc tcaaaccagt gttttgagca acctgcggct
    14281 agctgcctag tttgctcttt gattttcatt gagtttcact ccactgtgac ttatggtcag
    14341 atgctgatac ttttattctt ttatgcaata tcaaatttta actggcctgc tttgacacca
    14401 atcttaagtg tgctgcctgc cactaaatct cccttgagaa gaagttctgc caacttgtcc
    14461 tccacttctg tttgcagggt tctgcgaagt gggcgagctc ccatctctgg gtcatatcct
    14521 tgatttgcta acaatttcag agctgaagct tgtaatttca agtcaatgcc tttttcagcc
    14581 aaacttgcca ctaaaggctt gaccataatc ttcaccactt cctgcatatg gtcactagat
    14641 aggctatgga agaccacctt ctcatcaata cggttgatga attccggtct ataagctttt
    14701 ttcagttctt caaacatgcg tttttccata ttttcctggt caaaacgaat atccttagcc
    14761 ccaaaaccaa cagtcttatc atcacgaagg gcagtcgcac ctaggttcga tgtcataatg
    14821 ataatggtat ttgaaaaatc gaccttgcgt cccttgctat ctgtcaagac accgtcatcc
    14881 agaacctgca agagaacatt aaagatatct gggtgagcct tctctacctc atcaaagagg
    14941 agaacggaat agggtttatt gcgaaccttc tctgtcaact ccccaccttc ttcgtaaccc
    15001 acatagcctg gaggagctcc atttagacgg ctggctgcga atttctccat atactcactc
    15061 atatcaaagc ggataagggc tgattcgtca tcaaagagaa cttctgccaa agccttggcc
    15121 aactcggtct taccgacacc tgtgggtcct aggaacataa aggaaccgat cggacgcttg
    15181 tgactgcgaa ttcctgactg attacggcga atggcacgac taatgcttga aacagcttga
    15241 tcttgaccga tgacacgttt atggagttct gcttcaagat ttaaatactt cttagcatcc
    15301 gtttgagtca gtttttgaac ggggattcct gacaagcgac tcaaggtggt caaaatatca
    15361 gactctgtca ccaagtcttt gtagacaggt acttcctctt cttttgcgat tagctgggct
    15421 gcctgtttcc acttgccatc catcagggcc ttgtcagctg gactcaaatc tgaatcgtct
    15481 gcttttacat gctttgcctt attttgcact gttgctgctg cttcgtccaa gaggtcgata
    15541 gcagagtctg gcaagtgacg actggttaaa taacgatgag ccatcttaac cgctgtttca
    15601 accgcttcat ctgtgatttg tacacggtga tgtttctcat aagtcgcctt caaaccttgt
    15661 aaaatagtca tactatctgc cacacttggt tcttcaatcg tcactttagc gaaacgacga
    15721 gaaagtgccg catctttttc gatatgtttt tgatattctt cctgagtagt ggcaccaacc
    15781 gttctcaaag ttccacgcgc caaggctggt ttcaagatat tggccgcatc cagagtcgaa
    15841 tcaatcccgc taccagaacc catgatggtg tggagttcat cgataaagag gatgacttgg
    15901 ccatcttctt caatatcctt gatgatatta ttcatgcgtt cttcaaagtc accacggaag
    15961 cgtgtccctg caacgacatt catcaaatca agttctaaca cgcgcatctt agccatttcc
    16021 gcagacacgt caccactagc aatacgctga gcaagaccaa gcgccagagc tgttttcccg
    16081 acaccagcat ccccaaccaa gacagggttg ttcttagtct tccggctcaa gatttgaatc
    16141 atacgtgaga tttccttgtc ccgaccgatg actggttcta acttgccaga acgcgcttgc
    16201 tctgtcaaat catgcgtata gtcctcgaga ccaccactag gagtctgcgg catgcccatc
    16261 atattggcca tagaattttg cttgtcagct actgtacgat ggcgttggcg taaagccttg
    16321 agatcttcac gagtccagcc tgcccgttct tctaaatttc gacgaagagc agcaatcttg
    16381 acctgatctt tcttgtcttc ataagaaaaa ccagccctct ccaagatacg agtcgccaag
    16441 gcattgccat catgcaaaat cgcatagagg acgtgctctg tccctagcac cttagcatgg
    16501 accactgacg ctacatactc tgcttcgtca aaaagaacct gcaaacgacg ggagaacggc
    16561 aattccgtaa aggtttcatc ctggctatag tccgtttcag tcagttccaa agccacctct
    16621 tctaaacggt ccatctcata cggataatca tttaaagttg cccctgctac actataactg
    16681 tgattagaca tggcaatcaa caagtgccaa gactctagat aacgagctcc aaaatgtcca
    16741 gcaaccatgt aggcactttc gatacattca ttcaatgctt ttgaatagtt catcttactt
    16801 cccttttcta tctacctctt gtatgatctg acgtagcatg tttgctcgaa caactggagc
    16861 ttcttctcct aaaacgcgat ccaaagctac tgattctagc aaattcatct cctgcttggt
    16921 catcaattcc tgctcaacca aaagctggag aatatcctca taaatttctt gactgactcg
    16981 ctcaccaatc gagtaaagca gctcccggag catttcatga tgactagaaa actcaatccg
    17041 tcctatacga atgtagcctc cgccaccacg cttactttca accaagtagc ctctactttc
    17101 cgtaaagcgt gtcttgatca cgtagttaat ctgactagga acaacctgaa aggtatctgc
    17161 caactgactc cgttgcaact ccacgatacc agattgatct aaaatcgcct tgatgtaggc
    17221 ctcaatatga tccgatgtat ttttaaatct tataacaaac ccacctctct ctttaaacct
    17281 tgaccttgtt tgactattat accgaaattt tctcattttt aaaagaaaaa gggcgctggt
    17341 aaaggataat cttcaccaac tccctatttt tctacttatc taagcctaat tctgccaaga
    17401 tttgacgttt gtaggcaatc ttttggactt ccttgtcctc atcttcagac caatctagtt
    17461 taatttctga aacaatctgc ccagggcgat ttttcaagat atagatgcgg tcgctgagat
    17521 tgagggcctc ctcaatacta tgcgtgatga tcagggttgt tagctgcaac tgcttgtgaa
    17581 tctcaagata ccaagcgtgg agttccatct ttgtcatctc atccaaggcg ctaaaggcct
    17641 catctaagag aaagagcttg tgcccaaaaa ggtaggtccg gagtaaggct acacgctggc
    17701 gcatcccacc gctaagttca tgaggatact tgtctcttac agctgtcagc tggaaggtcg
    17761 caagaatttt atccgctcgg gaaatagctt ctgccttatc caccttttga atcaagaggg
    17821 gcagaatgat atttccaagc accgtcttgt gctccaagag cagatccttt tgcaacatat
    17881 aactcacgcg ccccttggga ttttcttcac catcaaggac aattctccct gactgaactt
    17941 ctaaaatccc agcgattaga ttaaagaggg tggtctttcc aacaccactt gggcctagga
    18001 tggaaaccac ttcgcctgaa gtcacctgta ggttgatatc ctctaaaatc ctctcctgac
    18061 cataggcata actgacgtgc tctagtctaa tttctgtcat tatttcacaa attcgttggt
    18121 gaagcctttg tctgtcaagt cttctttaag gataccattt tctttatccc atttgtagaa
    18181 agcattccag cgagctgcgt caaattgacc ccatttttcc ttgtcgcttg cgtattcttt
    18241 tgacaagtat ttttgagatt cgatgacaaa gtcacgtttt tccttgagtt caggtgcatt
    18301 cttgatgaga atatctgcag cttcttctgg atgttccatg gcatattggt agcctttttt
    18361 gatggcttgg atgactttgc gagcttcttc tttgttatct ttcagatagt cgttgtttgc
    18421 gatgataact ggtgaatagt agtcaaactc cttgacatag tctttcaagt acatgaagtt
    18481 agcatctaca ccttgagatt tagcaaggat accatcccaa ccgtagtaaa tccaagcagt
    18541 atcaaagacg ccattggcaa tcggtgtgat tgagtttgag tcgttatttg gtactttttc
    18601 aaccttctca aagtctccac cttgagattc taccaaggtt ttcaacatag caagttcagt
    18661 tgggtcattc catgtcccat atttcttacc aaccaagtct tttggactgc ttacattatc
    18721 agatttacga gagatgattc ctgatgtatt gtgttcaaca atagctgcaa cggcagtgat
    18781 tcctgctcct ttttccaatt tcttagccat gtagtcttgg aaatacactg caaatggtgc
    18841 ctttccgttg ataaccaagt cagaagaact ttcttctggt ggcaatttca aatcaacatc
    18901 cactccagct tctttgaaat aacctttttc cttggcaaca taaagccctg tgtggttggt
    18961 atttggtgtc cagtctagga taaagtcaac cttcttaagt tctgcctctt tgttgtcttt
    19021 agaagcagtt ccttgaccac aggccacaag cacaacagct acaagagctg ttacaagcgt
    19081 taaaaacact ttccatgttt tcttcatttt gatttcctct tttctttttc agaaacattc
    19141 taattctacg aacgtttcca tttaatcaca tatttttcac taatatcgac caacttcata
    19201 cccaaaaggc tgataatcga taccagaata ataatagcga acatggtatc atactgaaac
    19261 aatttcttgg actgaatcat ataaacacca agcccctcaa agcctcccaa ccactcagat
    19321 accactgttg tgataaaagc gtaggagaca ctgaccctca gaccagcata aaagtagggc
    19381 aggctgactg gaattttaaa atgccacagg atttgccaag gcttggcccg catcagacta
    19441 aacaaggtca gcatatcctt gtcgcaatgc ctaaaaccgt ccaaaatact aacgatgatg
    19501 ggaaaggttg tcgttaagat aatcaagaca atcttgggca aaatcccata acctagccac
    19561 aagaccagga taggagctat ggcaatggtc ggaatggtct gaatgaccac catcataggg
    19621 taaatcaggt cattgagcca agtcaaacta tccatgagca cagccataag acaggcaatc
    19681 aaaactccca aaatcagccc cagtaaagcc actctcaagg tcgcccagct atggtgccag
    19741 agaaattctc tgtcacgaac aaagggctgg agaatttcaa gaggtgtcgg caggataaac
    19801 ttggggagaa gtttaagaaa acctgctaac tgccagattg acaagactcc gagaaagccc
    19861 aatagactaa tgtggcgtct cagtatactt ctcaagtttc tcatcaatac ttaaaatctc
    19921 tcctacattt attttgacat tggcaaagac attgtctgcc tcctgccctg ccacttccag
    19981 cgcttctttt agaatgcgca taagctcatc aaactcccct tccaagaccg tttcaaatgg
    20041 tgtcactacc atcgtcactt cttgagtttg cagataagca atgacctgat caataacagc
    20101 tatccgatca atcccctgtg ctaggggtaa aacttgcaag gcaatgcttg ctttcataag
    20161 aacctcctct tctcgttttc cttttaaaaa aaaaagaaaa acctttacca tacatggaaa
    20221 aggtgcaaaa ttagactaag tatcgttccc tccgctggca ttacccagat caggtgcggt
    20281 cgaagtttaa cacttcctct cagactgtac acagactccc atatattata tgtatatatg
    20341 atagcgcaaa ataagaaaaa tgtcaaggaa actgcttaca gaaaagagca ggaaatttct
    20401 cctactccaa ttttctatac tcgttctcca tcactgttta ctctgtagcc atccaccgtc
    20461 gtattaacag ctaaggcgcc tgaagagtaa gaataatacc acttaccaga gacctggtac
    20521 cagcctgttt tcattgcccc tgaactaccc agataatacc acgagccatt ctcctttaac
    20581 catcctgtct gcatttcacc agataatttc aaataatacc agctagaacc gtctttcagc
    20641 catcctgttg atttagaacc atttgatttg aaatgatacc aactaccatt tattttcttc
    20701 caaccgacag ttgaactatt attggatcca tagctagaga tatttcctga ttgattctga
    20761 taattttgtg aagaagtcac tccctctagc tgtatatagc gacgacttcc gttataagcc
    20821 gtataactca accacttgta gccgtctttt tcaagtatct gatcataatg aaccttctct
    20881 ccaggataat agtaatcaat cacagttgca ctaactaggg gttcagtttt gatagcagac
    20941 ttggtcttaa aataatgagt tcctcctgtt gaagaaagaa cagaatgacc tagaggattt
    21001 ttattcacag cctccaattg aacataacga tagcttccat tataggcagt ataactcaac
    21061 cacttatagc cgtctttttc aagtatctga tcataatgaa ccttctctcc aggataatag
    21121 taatcaatca cagttgcact aactaggggt tcagttttaa tagcagactt ggtcttaaaa
    21181 taatgagttc cgcctgttga agttgaggat tgactattcc caacactgcc accatccaaa
    21241 tctttaaaat gaataaatcc tgtcatcgtg tttgctttta taactcgttt attataggat
    21301 tctgtataac catagttata ttcctcaatc tcaatctgat ctcccattac atttgacacc
    21361 caggcaacat gaccataagt tcctgcagta gaccaagtaa tggaaccaat cgtcggtgta
    21421 ttatctacac gataaccttc ccgacgagca cgatgtcccc attcattcgc atttccataa
    21481 gctgccggaa tttcaaaacc attgacatta ctcaaacgaa aggctacaaa agaagtacac
    21541 tgacgagaat acatgcgcca ctgatcaatc tcctggctcc catttttata ataagcagga
    21601 taatcatccc cacgcgcaat cgatccattt cctctggaat aggcatagac actatcacca
    21661 gctgctagca tcaatcctac tactaaaaaa ggaacaagct ttttaactga catcttcaag
    21721 taataacttg ttcctcttgc tataaacggt aaaattttca ttcttcctcc ttgaaaaata
    21781 atataaagcg atgattgcct tcacctaaat tattcggaga aaaaagaaaa agtgagaaaa
    21841 tttctcactt tagtcaagat aatgaatcaa attcttttct gtaagaatat ctgagtaagt
    21901 aaaggattca acgtaaacat taacttgttt atctccttcc acatccccaa actccacaat
    21961 aaatagggat ggataaactt caattagctt acccaatcta tttttttggc gcttacgacc
    22021 attctccaaa gtcatttcta cgacttgtcc ctcatgtgcc ttgatttctt ctttgatttt
    22081 tttcatcttg gctacatctg taaatgcatc tgacatctta tgcctccctc tttgagatac
    22141 ttgaaaattt attgtattct ttttggaaaa tcaattccac tgttccacga gctccactac
    22201 ggtttttctc gataataact tccaccttat tatttgggat accctcctct tcttcaccac
    22261 cacgttcata gtagtcatcg cgatagagaa aagctacgat atcagcgtcc tgctcaatag
    22321 acccagattc acgaatatca gacaagaccg gtctcttgtc ctgacgttgt tctacaccac
    22381 gagaaagctg actcagagcg attactggaa ccttcaattc cttggctagt attttcaact
    22441 gacgagaaat ttcagaaact tcttgttgac gattttctcg accagttccc gtgataagtt
    22501 gcaaatagtc tatcaaaatc aaaccaagat ttccagtttc ttgagccaat ttacgagaac
    22561 gagaacgaat ctctgtaatc cgaatacctg gcgtatcatc gatatagata ctggcgttag
    22621 ctagattacc ctgagcaata gtatattttt gccactcctc atctgtcaat tgccctgtac
    22681 ggatagaatg tgactccacc aagccttctg cagctaacat acggtctacc aagctttccg
    22741 cacccatttc gagtgaaaaa atagcaaccg ttttgtccaa cttagtccca atattctgag
    22801 cgatattcaa ggcaaatgct gtcttaccaa ctgctggacg agctgctaag ataatcaact
    22861 cctcctcatg aagtcctgtt gtcatatgat ccaaatcacg ataacctgtc gcaatacctg
    22921 taatatcggt cgtttgttgc gagcgagctt ccagatttcc aaagttgaga ttcaacacat
    22981 ctcgaatgtt cttaaacccg cttcgatttg cattttcact gacatcaatc aacccttttt
    23041 ctgcctgagc aataatttca tcagctggtt gtgacgcttc gtaagcttgg ttgacagact
    23101 ctgtcaactt ggcaattaaa cgacgtagca ttgctttttc tgcaacaatc ttagcataat
    23161 actccgcatt agcagaagtt ggcacagaat taacaatctc aaccaagtaa gacaagccac
    23221 caatattctg taaatcacct tgattatcaa ggatagtacg aaccgttgtt gcatctatgg
    23281 catcaccacg atcggataaa tcgaccatgg cttggaaaat caaacgatgg gcatacttaa
    23341 aaaagtcccg agactcaatg tattctcgca caaaaacaag tttactctca tcaataaaga
    23401 tagcccctaa aacggattgc tcagctaaga tatcttgagg ttgtactcgt aactcttcta
    23461 cttctgccat cagatttccc ttccttttac aatcttgtca agaaggtgta aacttatcct
    23521 tctttcacac gaagattgat tacacttgtg atatcttgat agattttcac tggcacatca
    23581 atcaaaccaa ccgctcgaat cggagcttgt acttgaatat gacgtttatc aatcttaatt
    23641 ccaaattgct tttgcaattc ttctgcaatc ttcttattgg taatagaacc aaaggtacga
    23701 ccatctggac caactttttc aacaaattct acaacagttt cttctgcttc aagttgtgct
    23761 ttaattgctt ttccttctgc aatcatctca gcgtgagctt tttcttccga tttttgttta
    23821 ccacgaagtt cacctacagc ttgagcagtc gcttctttgg ctagattctt tttgataaga
    23881 aaattttgcg cataccctgt tggtacttcc ttaatttcgc cttttttacc ttttccttta
    23941 acatctgcta aaaagattac tttcattctt ctttctcctt ttcctttatt tcatttaata
    24001 caatttctgt cagtttttca cctgcttctg acaaggttac atctttaatt tgagctgctg
    24061 ccaaattaaa gtggcctcca ccgcctaact cttccataat ccgttgtaca ttcagtttac
    24121 tacgacttcg agctgagata gagataaatc cttgtatatt cttcgcaaga acaaaactcg
    24181 cttcaatacc tgacatggct aacatggcat ctgctgcctt actaataaca actgtatcat
    24241 agcatttcgt gtccttagcc tctgctatta gtacatctga acctaattta cgcccctgta
    24301 aaataagttc attgacctca cgatattctt caaaatctgt cgcagcgatt tcctggatag
    24361 caatactatc acttccgcgc gttctgagat agctagcaac atcaaatgtc cgactagtta
    24421 ctcgcgaggt gaaattttta gtatccaaca tcataccagc catcaaaaca cttgcttgca
    24481 tacgactcaa acgatttttc ttagaattct ggaactgaat caattccgtt accaactcac
    24541 tggcactact tgcaccactt tcgatataag taatgactgc attatctgga aaatcctgat
    24601 cccttctatg gtggtcaata acaatggttt gggtaaataa atcataaaat tcttttgata
    24661 atgttaaggc tgtctttgaa tggtctacaa gaatcaacaa agaacgattg gtcaccatcc
    24721 ccattgcatc cttaacagac aacaacttcg taactccttc tttttctatg aatgaaacag
    24781 ctcgttcaat atctggagac atttgttctt catcataaag agcatagcta ttttcaatca
    24841 cattgctggc gaacaactgc atacctacag cagagcccaa agcatccatg tctaaatttt
    24901 tgtgaccgac tacaaaaacc tgatctacac tccgaatctt atctgaaata gctgtcatca
    24961 tagcgcgcgt acgagtccgt gtacgcttga ttgaagcagc agacccacca ccaaaataaa
    25021 ctggattttt cgtttcgtcg ttttccttaa caaccacctg gtcgccacca cgtacttcag
    25081 ccaagttcaa attgagcaaa gcaactttcc ctatctcatc atgatttcca tcgccataag
    25141 aaaatcccat acttaaggtc aagggcaact gtctctgttt cgactcttct ctgaaagcat
    25201 caataacaga aaatttatca ttcatcaagc cctcaagcac cgtgtagtca gtaaatagat
    25261 aaaatcgatc catacttacc cgacgagaaa acatcatgta tttttctgaa aactctgata
    25321 taaaattagc tacaaaacta ttgatttgac taatatctga ctcagaagtt gcatcctcca
    25381 aatcatcata attatccaca gagacaatcc caatcactgg tcgacttgtt accaattcat
    25441 ctgttatggc ttgttccctg gatacatcta caaaatacaa aacaccggaa gaagcatcca
    25501 tatgaacagc ataacgcttc tcaccaagct tggcataagt agacggattt cctactgaag
    25561 ccttgataat cgtttgaaca gcttctaaat caaaatcacc atcttccttg gtcaaaatca
    25621 attcagcata gggattaaac cactcaacct ctccagaaga taaattcaat ttcataacac
    25681 ctacaggcat ttgttccaat agagatgtca aactttcttc cgcttggtgg tttacatact
    25741 gtatctgttc tacatcactc cttgtataat gcactctcag tttcttaaat aaaaaaacat
    25801 agcctcctac aaaaagaaac aaaattaaaa ccgtcaacag attattatta acaaaaataa
    25861 tgaaagtgga taagactcca aacgcaatca atcctactag aataggaaaa attggactta
    25921 cataaaattt tttcattcaa aacctcttgg cacccattat accataatac ccctcaaaaa
    25981 gcgacttttt aaaggtgtaa tcagtaattc tatcaattat aagaaaaagg tagtttacaa
    26041 ttcagtaaac ctacctttac acatattgaa attaagattc tttaacctct aacaaaccaa
    26101 tttcgccatc ctcacgacga taaatcacat tggttgtctg atcttcaaca tccacataga
    26161 taaagaaatc atgccccaat aaatccattt gtagaattgc ttcttccaaa tccattggtt
    26221 ttaaatcaat ttgttttgaa cgaacaactt tagactggac aacatttgaa tcttccacca
    26281 aagcatctgt aaataattga ccagttgcta ccttattttt atttttacgc tcgatttttg
    26341 ttttattttt acgaatctga cgttcaattt tatcagttac aaggtcaatt gaaccataca
    26401 tatcttgaga tacatcttct gcgcggagag taatagatcc aagcggaatc gttacttcca
    26461 ctttagccgt tttttcacga taaactttta agttaattcg ggcatccaac tcttgttctg
    26521 gttggaagta cttttcgatc ttttcgagtt tagaaactac ataatcacga attgcttctg
    26581 ttacttctag gttttcacca cggatactat atttaatcat atgaatacct tctttctaaa
    26641 catttttgtt tttatgattt tattataacg ctttcattct atttttgcaa attttttcct
    26701 catcttacaa gggaaaatgt ttttacatcc ttagcaccgg cttcttccaa cagtttctta
    26761 acacgattta tagttgctcc tgtagtatag atatcatcta taagtaggat ttttttagga
    26821 atagtgactc cacttttaat aaagaaagga agttctgtcc ccaagcgctc tgaacgattt
    26881 ttagaagaac tggctctctc ttctcttttc tctaataaat ctagatactc aaagcctgct
    26941 gcctctacca agccctcaac ctgattaaat cctctgttag catatctatc aggacttagg
    27001 ggaattacaa caaattgata ctctttgtac tttttcaact cctcacttaa aaatgaagcg
    27061 aaaacttttc ttaacaggaa gtctccatca aacttatacc gactgaaaaa atccttcata
    27121 gcttgattgt aagtaaaaat cgctctatga ctgacttcaa ctccttcttt acaccaaagt
    27181 tgacaatctt gacactttgt tgacaactct gttttcatac aatttggaca gttctcttcc
    27241 ccaattcttt caaaagtaga atcacagtct gaacaaagac aagagtcatc attcctcaga
    27301 agtaagagac tactaaaagt taaaacagtc ttcatagtct gcccacataa caagcacttc
    27361 atagaccagc ctccttattc atcatctgaa tttccttaat cgccttcttg attgaagcat
    27421 ttaacccatc atggaagaaa agcaaatctc ctgtcggtct atccatgctt cgtccaactc
    27481 gtccaccaat ctgaatcaaa ctagacttgg taaacaaacg atgattggcc tctattacga
    27541 aaacatccac acaagggaag gtaactccac gctccaagat tgtcgtactg ataagtattg
    27601 tcagttctcc atctcgaaaa gcttgtactt gctctaatcg atcctctgtt acagaagata
    27661 caaagccaat tttctcattt ggaaattgct cctgtaagat ttcttctaac tgctcccctt
    27721 tcttaatttc cgaagcaaaa atgagtaacg gataagctgt ctttctctgc ttctcaatat
    27781 aggactttaa ctttggtgac aaacgattct tgtctaagta gcgattaaaa tccgataacc
    27841 aaattggttt tggaataatc aacggatttc catgaaaccg tctcggtaaa ctcagtcttt
    27901 ttagttctcc taaacggacc tttttatcta actcattggt cgaagtcgct gttaaaaaga
    27961 ttctcaatcc attctccttt acactattct tgacagcgtg gtaaagcgtg ggattatcaa
    28021 cataaggaaa agcatctact tcatccacta tcagcaaatc aaaagcttga taaaacttca
    28081 ataactgatg ggttgttgca acaactagtg gtgttcgaaa ataaggttcc gattctccat
    28141 gtagcaaagc tataccgcaa gaaaaatcct gttgcaggcg cttgtacagc tccaaacaaa
    28201 catctatgcg aggactagcc aaacacactg caccacccgc attgatcact ttagccacta
    28261 cttgataaat catttctgtc tttccagctc ctgttaccgc atgaactaag gttggcttct
    28321 gcttgtctac tgcttgaagc agtccctctg acaccttctc ttgaaaagga gttaattggc
    28381 cgcgccattt gagaacatct tgcttcggaa aatcctcctg cggaaaatag tataaagttt
    28441 gatcacttct gactcgcttc atcagcaagc actctcgaca atagtaagca ccgatgggca
    28501 aataccattc ttctagaata gtactattac agcgttgaca gaaaagtttc cccttctcct
    28561 ttctcattgc tggaagtttc tccgccaact gacgttcttc ttctgttaat tcattctcag
    28621 taaataaacg accgagataa tctaaattta ctttcatact tctttattcg taaaaactag
    28681 cactttagat tattttttag tacaattaaa tcatggaatt taggacaatt aaagaggacg
    28741 gccaagtcca agaagaaatc aaaaaatctc gctttatctg ccatgccaag cgtgtttata
    28801 gcgaagaaga ggctcgtgac ttcattactg ccatcaaaaa agaacactac aaagcgacac
    28861 ataactgctc tgcctttatt attggagaac gtagtgaaat taaacgtaca agtgatgatg
    28921 gtgagcctag tggtactgct ggtgttccca tgcttggggt actagaaaat cacaatctca
    28981 ccaatgtctg tgtggtcgtg acacgctact ttggtggtat taaactaggc gctggaggac
    29041 taattcgtgc ttacgccggc agtgtcgcct tagctgtcaa agaaattggt attattgaaa
    29101 taaaagaaca ggctggcatt gctattcaaa tgtcttatgc tcagtaccaa gagtacagta
    29161 acttccttaa agaacatgat ctcatggagc tggatacaaa ctttacagat caagtcgata
    29221 cgatgattta tgttgataag gaagaaaaag aaactattaa agctgcactt gtggagtttt
    29281 ttaatggaaa agtcacttta actgaccaag gtttacgaga ggttgaagtt cctgtaaact
    29341 tagtgtaaac aatgaataat acagcgtttc gttgacattc tcacaactac tttagcgagc
    29401 aaaataaaaa gatgcgtacc aaaatatact agaaaatgaa gcaattcaaa cgaaacctga
    29461 tatcgttttc cttcacacct atttactaga attagctgaa cgcaatcact tgaaaattaa
    29521 tgactttgat ctatgatata tagaaatcct atcgcttcga taggatttct atattttaca
    29581 aatggtatag atagcgttat actaaagata tcttatacaa agaggtattc atatgtctat
    29641 ttataacaac attactgaat taatcggtca aacaccgatt gttaaactta acaacatcgt
    29701 gccagaaggt gctgcagacg tctatataaa gcttgaagca tttaatcctg gttcatctgt
    29761 aaaagaccgt attgccctta gcatgattga aaaagctgaa caagatggta ttctgaaacc
    29821 tggttctact attgttgaag caacaagtgg aaacactggt attggacttt catgggtagg
    29881 tgctgctaaa gggtataaag tcgtcatcgt tatgcctgaa actatgagtg tagaacgacg
    29941 taaaattatc caagcttatg gtgctgaact cgtcctaact cctggtagcg agggaatgaa
    30001 aggtgctatt gctaaggctc aagaaatcgc tgctgaacgt gatggtttcc ttcctcttca
    30061 atttgacaat ccagctaatc cagaagtaca cgaaagaaca acaggagctg agatactagc
    30121 tgctttcggt aaagatggat tagatgcctt tgttgctgga gtaggtaccg gtggaacgat
    30181 ttctggtgtt tctcatgcac tcaaatcaga aaattctaac attcaagttt atgcagtgga
    30241 agctgatgaa tctgctattc tatctggtga aaaacctggt cctcacaaaa ttcaaggtat
    30301 ctcagctgga tttattcctg atacacttga tactaaagcc tatgatagta tcgttcgtgt
    30361 aacatcagat gatgctcttg cactcggacg tgaaattggt ggaaaagaag gcttccttgt
    30421 agggatttcc tcagctgcag ctatctacgg agccatcgag gttgccaaaa aattaggtac
    30481 aggtaaaaaa gtccttgctc tagcaccaga taacggtgaa cgttatctct ctacagcact
    30541 ctatgaattt gaagtttagt ctcctaaata caattggccc ttattatagg gctttttatt
    30601 tttaccttga aaataggaag ctctccctat aaggatcgac tgcatacaaa aaaggaagca
    30661 atttgcttcc tttctattat tagttattca aggctgctgc cattgtagct gcaacttcag
    30721 cttcgaagtc gtttgcagct ttctcgatac cttcaccaac ttcaaagcga gcaaactcaa
    30781 ctaccgaagc gttaactgat tcaaggtatg cttcaactgt cttgctgtca tccatgatgt
    30841 aaacttgtgc aagaagtgtg taagcttggt caactttagt gttatcaagc atgaagcgat
    30901 ccattttacc tggaataatt ttgtcccaga ttttttctgg tttgccttct gcagccaatt
    30961 cagctttgat gtcagcttca gcttgagcaa taacatcatc agttaattga gcttttgatc
    31021 catacttcaa gtgtggaaga gctggtttat taaccattgc acggctttcg ttgtcttggt
    31081 cgataacgtg attcaattgt gccaactcat ctttaacgaa ttgctcatcc aattctttgt
    31141 aagaaagaac tgttggtttc atcgctgcga tgtgcattga caattgttta gcaagtgctt
    31201 cgtctccacc ttcaacaact gaaataacac cgatacgtcc accgttatgt tggtatgctc
    31261 caaagtgttg tgcgtctgtt ttttcaatca atgcaaagcg acggaatgag attttctctc
    31321 cgatagttgc tgttgcagat acgtatgcag cttcaagagt ttcacctgaa ggcattatca
    31381 aagcaagagc ttcttcattg ttagcaggtt ttccttcagc aatgacttta gctgtagtat
    31441 ttaccaattc aacgaattga gcgttttttg caacgaagtc agtttcagcg tttacttcaa
    31501 taactgctgc aacattaccg ttaacataaa caccagtcaa accttctgca gcaacacggt
    31561 cagctttctt agctgcctta gccatacctt tttcacgaag caattcaatc gctttttcga
    31621 tgtcaccgtc tgtttctaca agcgcttttt tagcgtccat aacaccggca ccagattttt
    31681 cacgcaactc ttttacaagt ttagctgtaa tttctgccat tttaattctc ctatattttt
    31741 tgaaaatagg agagccgggc taagccccgc cctcctaggt aattactatt tgtataaatt
    31801 aagcgttgtc accttctaca acttcaacga tttcttcaat tgaatctgct tgagtttctg
    31861 aagctgcaaa ttctgcttca actgctactg catcctcacc ttgacgtcct tcgataatag
    31921 cgtcagccaa tttagctgtg atcaatttaa cagcacggat agcgtcatcg ttagctggga
    31981 tgattacatc gatatcatct ggatcagtat tggtgtcaac catcgctaca actgggattc
    32041 ccaatttttt agcttcttta acagcgattt gctctttatg tgggtcaact acgtacatca
    32101 catctgggat acgaggcata tcttcgatac cacccaagaa tttttcaaga cgcgcacgtt
    32161 gtttgttaag aagtgcaact tctttcttag gaagaacttc gaaagttcca tcttcttcca
    32221 tacgtttaat ttctttcaaa cgagcgatac gtttttggat tgttccccag tttgtaagag
    32281 ttccacccaa ccaacggtgg ttgatgaagt attgacctga acgtactgct tcttcagcaa
    32341 ctgcatcagc tgcttgtttc ttagtaccaa cgaacaatac aactgcatcg ttagctgctg
    32401 catcacgcat gaagtcgtat gcttggtcag cgtattttac agtttgttgc aagtcgataa
    32461 cgtggattcc gttacgttca gtaaagatgt acttagccat cttaggattc cagcgacggg
    32521 tttggtgacc aaagtgtaca ccagcctcaa gaagttgttt cattgaaatt actgccatga
    32581 gtatttctcc tttttgtttt tttcctcttc ttgacttcaa ctcgcaaaac gacccaaggg
    32641 caactgcttc acaattcatc aagaatgagt attatcgttc acacgacaat tttcatttta
    32701 ccatactttt ccaatatttt caagctattt tgatataatt tttaataggg attgtcacgg
    32761 taaaacctga aaagagggct ctattcgagt ccctctgtaa attaatctgc ataaatatat
    32821 gtaacaaaac cttcagaagt tgttgttgga ttgaaccatc cacggtgatt tccaattgta
    32881 cgattacctg cataatttga ttctgatact tggatacgtg ttgttgattc aacagctgta
    32941 acaaccgcta cgtgaccata tccaccatca ttccaacatg caattgctcc aacttgaggt
    33001 gttgaacctg tacggaaacc tgctgctgct gcacttgtag cccactgtgc tccattaccc
    33061 cagtagtctc cagcccaagg tgccaatgtt tttactcccc atgtacattc tccaattgga
    33121 taacttgaag cgtttgtact gtatgttgga cgaacttttg cacggacagg tgctgctgca
    33181 gattcagata ctgcttgcac ttgagctgtt aagttagtgt ttgctgaagc aagtactgat
    33241 tgttgttggc tagctcgttt ttctttataa gctgcttctg ccaccgcagc tgcacgagcc
    33301 tctgcctcag ctgctgcttt ttgctctaat agacttgctt tttccccttc agctgtcgct
    33361 ttctcagcag caagacttaa ttcagcagct tttagttctg cctgtttcgt agtcaatgct
    33421 tgagcatcat cagccaattt ttgttgatta gcaattacaa tattgatagc atcattattt
    33481 gctacttgtt tttcagaaat agctttttta tctgcctttt gttgttctaa cattttgttg
    33541 tttgcagata cgatttcact cattgcagca acacgtgaaa tagcttctgt aattgatttt
    33601 gagtttacaa tggtattgat atagctagtt acggctccat ttgtttgagc actacgagct
    33661 tgtttttcca acgattggtt acgagaaaca atgtttttag aaagttctgt aatctcaccc
    33721 tcgagtttct tagattctgc ttgtaatcta tcattttcag cttgcaagtt agactgctca
    33781 gcttgaatag ctgatacttg ctcctgaatt tggtcaactt gtttttgggc ttcttgttgt
    33841 tgtgctgtta agttactaat tttattatct tgagcagcaa ttttgtcatc agtcgtttct
    33901 gcatgcgcag ttgttaaaac agctacttga gaaaccatta ctgtacttaa taaaagtgac
    33961 gctaagattt ttttcttcat attacgtaga tactccttct tttaaagaca atactagtat
    34021 accaaaaaaa ggcctaataa atattacgcc tttgttacat ttatatgtcg ttcttataga
    34081 taatattttt caaaaataaa ttgaaaaaca gtaatccata aaaaatttaa aatcatcgtc
    34141 ggtaccaagc tatagactat aaaaatcgac atgctatcta ctgtcaaccc taccataaaa
    34201 gccaaaagat aactacccat ttcaaacagg aatgtcagca caatcatagc tagcattctt
    34261 gtccaacgat tcaacaaaat aacactattc aatttatgaa ggaaggctcc caataagata
    34321 aataagagag ttgtaatccc tattagatgg aaaaagtaaa catcataaac caagcctata
    34381 acaaaacaat agactaggta gagatactct gatacttcta tcgtctcaaa taagagaaat
    34441 agaaaaagaa aatgactagc caaatgtaca tgggggaaaa atgagcccag aagctggcta
    34501 atatgggcgt caattagaac aaagaaagga agcaataaaa atactccaac tcgcttcaac
    34561 tgtctcatta tgaattcccc actaattcta tcacatctac attatgagta tctgcactca
    34621 atttaacagt tacttctcgt gtcaaatagt ctgtactatg cgttgtggca accacttcac
    34681 caacaggaat atcagcaacg ttaaagtttc ctaatccacc agtagtcacc ttatctcccg
    34741 cactaatatc gctattacta tttaattggc taattttaag aacgtcattt tccttgtcat
    34801 agccaataat aattccataa attgtagtag agccatgttg aattttaaca gaaatcttat
    34861 cagcattttc cgtatttgtc agaaggttga ctatagtaga gttctcctct acttttgaaa
    34921 cactcccaat caagccacca tttgcaatag ctaacatgtt ctcagaagca ccttttgatc
    34981 tacctgcatc taaggtcaac tcctgcttcc aagataccgg agaacgcata ataacatctg
    35041 ctgctaaagt ctttgtggct tgcaatttag acttcatatc aagcaattgg cgcagttgtt
    35101 cattttctgt ctttaaactt tccacctcat ttgatttaac ttctaattgg taaagctgtt
    35161 tcttcaaact ttcattttca ttatatgttc gtgtcaaatg agccaaatct gatttgacag
    35221 aatcaaacca ctgaaaaggt ttttgtacaa ctctatcaac caatgagatt ccatctccta
    35281 attttgtcac aattgtactt gaataagtcg tcgctaagag agctgacaca agcagaacag
    35341 tgacaaaaac aataatgaca tattttgatt ttttaaaacg gttcatattc ctacctttat
    35401 atcaaaaact gttacagtaa ctttttatca attcctgaaa gctactaaga ttttaagaaa
    35461 aataaacaac aaccaagtac gagaataaca agaatggtca gcgtatcctt tagagtccat
    35521 ttcaattgtc ggtattgact tctgcctttt ccaccctgat agccacgcgc ttccatagcg
    35581 atagccaagg aatctgcacg ttttaaactt gtcgcaaaaa gaggaatcaa aatgggaatc
    35641 atcgccttta ctttttgaac gatgcttcct tctccaaaat ccactccacg agctttctgt
    35701 gcattcataa tccgcgtcgt atcatccatc aaggttggga caaaacgcaa actcatagac
    35761 agcatcaatc caatttcatg aactggaact ttcacacgct ttaaaggtgc taataaagct
    35821 tcgacagctg atgccaaact taaaggcatg gttgttaagg ttaacaaagt tgaaaagaaa
    35881 ataatcaata caaagcgaca aaaaataatc ccagcttgtt gcaaagcata atccgtgatt
    35941 ctcacaaacg aaaactcaaa taaaacattc ccattagaaa tgaaaaatag ttgaaaaata
    36001 gttgtgaagg caatcaagaa aaacatagac ttcaagccct gaataaaaaa tgaaagagat
    36061 actcctgaca aggcaataaa tatccctgtc gctataaaaa gaatcagatt cgtcaagggg
    36121 ttattagccc aaaacacaat caaaatcagt agcatcatag caagcaattt gctacgtgga
    36181 tccaatcggt gaacaatcga atcccctggg atataacgcc ctaaaatcat actatccatt
    36241 tagcgactcc ttgaactcct ctatcttaat cggtaatcgt ttaaatgaca cgcctctatc
    36301 agccaatcgt ttacaaaagg ccgtaatttt aggtactccc aactgaactt cttccataaa
    36361 aacaacgtct tgaaagacat cacttggttt gccccccttt actaaacgtc ccttttccat
    36421 tacatagact tgattcgcat attcagcaac atcatccatc aaatgcgtta ccaagacgat
    36481 ggtcatccct gactggtgga gttttttgaa cagggtcatc aactcttttc tccctagagg
    36541 atctagacca gctgttggct catctaagac taatatagct ggctccatgg caagtatgcc
    36601 tgcaatggca acacgtctca tttgtccccc tgacagctca aacggactac gatcaaaaag
    36661 tgattcatca attccaacca gagccagttt ctcacgcgca gtcttcacag catcttcttc
    36721 agaaactcca aaattttgcg gtccaaaagc aacgtccttc aaaaccgttt cttcaaaaat
    36781 ctgattttca gcaaactgaa ataccaagcc aacctgtttt ctaatttgac gaatatcttt
    36841 atttttagaa gtcgaggtga ttaaggtatc aaaaaccctc acactccctt gatttggcac
    36901 caataaacca tttaagagtt gtaaaatagt tgatttacca ctacctgtgt gcccaattaa
    36961 agctgtataa gagccatctt caatcgtcaa agaaacatcc gacaaagctg ctgaagctaa
    37021 gggagtacct tcttgatatg taaaattcac attttctaga gcaattccca tagcttatcc
    37081 tctagctcac tttctgtcaa ataattttca ggtaaatcat agccattctg gctcaaagat
    37141 ttttttaatt gattagcaaa aggatcgtct aatccaattt gatctaaatc atttcgagag
    37201 aaaagctccc ttggactact agttgattca atttcccctt ttttcatgac caatacgcga
    37261 tcactcatgg cgacttcttc caaatcatgg gtaatagaaa tgaccgtcat atcatagtct
    37321 tttcgaattc cttttactgt cccaataagt tctctacgcc cctcaggatc caacatactc
    37381 gttgcttcat ctaagattaa aatagctggt cttagggcta caacacctgc aatggccaca
    37441 cgttgctttt gaccacctga tagacgcgct ggctctctct ttttaaagtc caacatgcca
    37501 actaaagcca gagcttcttc cactctcttt ttcatttctt gacgagaaag tccctgattt
    37561 tccaaaccaa aagcaacatc atcttcaaca gtcgctccaa caaattgatt gtctggattt
    37621 tgaaaaacca taccgatttg acgacgtata ttccaaacat tttcctcagt caaacgttgg
    37681 ccatcaatta caatctctcc ggattctgct tccagtaagc catcaattaa tcgaaccgtc
    37741 gttgatttac cactaccatt atgccctaca atcgaaagcc attctccacg tttcacgtga
    37801 aacgtaatat ccttcacatc gtagtagttc tgattttctt tatagcgaaa agaaagattt
    37861 tttacatcaa ttattgattt catttcgaac caaatgtccc tttaaataca taggcactac
    37921 ccttaaaata gtcatagcca gagtagatag tgaaaaataa ggctacataa agtagaactt
    37981 gaccaagcaa agtccaatgt aatagcaaga aaataatagc aaacatctga ctaaaagttt
    38041 taatttttcc aggcattgct gctgctaaaa ttgttccacc agtttcaacc aataaaagcc
    38101 ttaaacctgt cacagctaac tcacgacata taatcactgc aacaatccaa gccggagcca
    38161 tacctaactc aatcaacata ataaaagccg acataactag taacttatcc gccataggat
    38221 ctgcaaattt accaaaatta ctgaccacat tccatttacg agctaaatat ccatctaaat
    38281 agtcggtaat actggcaaca gcaaagataa tagctgcaac tatatgactc tctatcgaat
    38341 ttcctatcgt taaaataaag ataaaaatag gtataaagag aattcgacct attgttaaga
    38401 gattgggaat ttgttctttt ttcattcgtt tttccttaat ttttagtaaa ggttacagtg
    38461 atttgtccag tctgagctgt taatttcgat aaatcaacag tctgattatc tactgtcaaa
    38521 tcaacacctt ttacaacacc taacgtaatt gttacaggac ttttagttgc aactgttgct
    38581 tctgcacttt tcttctttgg cgataaggtt acaccgccct caagttcgct ttctgaaaca
    38641 ctgacccaac ttgtaacatc tgaaactgcc aattgcaatt taactgtttc cttacttgtc
    38701 ttataagcga tttctacatg atttccttcg cctgatacac ttatagctga ttctatacta
    38761 gaagaactac tacttgagga gtggggaaca gagctagttg aacttgttga ttgaaccaca
    38821 ctgtaattag aaagagaagg ctcctctggt tgagtttgaa tatagttcca aacataataa
    38881 gtcacaaaaa ttaaaatcga taaagcaaaa aggataaaat aaaataaagg taaaaatgat
    38941 gtttttttct tctttttctt acttgaacgt ctacgacctg tcaactcatc ttcatcaaca
    39001 tctacttcct cataagtaat catactccca gaatcataag catccaaaac aatttggtca
    39061 tctaactcaa cagcccatgc atattttttc aagaaagaac gcgtgtaaaa aggacttgga
    39121 agttgatcga aatcgtctgc ttccattgct tccaacatat ctaactggat ttctgtcttt
    39181 ttctgcaatt catctaaact caatccctga ttgattctag ctaatcgtaa aacctctcca
    39241 attgtttttt ttctcatact tgtcatccct tctttctagt gtctattatg ataggttact
    39301 acgatggaaa tattgtaaaa tcaactatgt caccatcatc tattaaatga tgtccagcat
    39361 caaggacatc ctctaaagta atttcctgta aaattttcgg caaatcaaaa attgtctcac
    39421 cattttcaaa agcatcatat tgcgttgcaa taaattcaag agagttcatg ctactgaaaa
    39481 attcgccaaa catctctctt ttgataatat ctaaatgttc ctctgtaata tctaaatcct
    39541 ttgtaaaatt acgaatagcc ttcctaaatt gatgagacaa agcaactggc tctttcgtat
    39601 ccattgtcaa catgacaaaa tgaaagcgac ttgttatttc aacttccaga gataaggacg
    39661 catcaatttt acctgattca taacattttt gaaaacgatc cgaagtccaa ccaaacatca
    39721 ttgcaaacaa taattttaat aaaatatgat gtcgatagca atccgcttca gaaacttctc
    39781 gcttacctct aactccaatc gctagtttgg gagaagatac ttccattctc atactatctg
    39841 ttagctttac aggctgtaaa aacaactttt ctcttgctac ttcctgaaaa tctgaatctt
    39901 tcagttcttt gctttcaaaa tagtcctgta ctcgctccac atcaaaatta ccaactaaaa
    39961 acagagacat gtttacaggt ttgtaaaact ttgtaaaatt ttcttgcaaa ttagttagat
    40021 tgatttggga aatggactcc tcacttccaa ctatatcagt tgctaaaggt gtaccaggat
    40081 acaaattcgc taaagttgaa aagaataaac acgaatctgg atcatcttgg tacatttctc
    40141 gttcttgctg aataatatcc tgctctgtca gaatggaagc ttcagtaaag tgtgctgatg
    40201 ttaccaattc atcaagtaaa tctaaatttt ctaaaaaata atccgttgct gaaaaaagat
    40261 agtttgtttt tgtaaagctt gtaaaggcat tactatctgc acctatactc gtaaaagccg
    40321 acatcaaatc actagaatct tctctctcaa ataatttatg ttcaagaaaa tgagcaattc
    40381 ctccaggata ttgttttaca tctccgtcaa cttctgtgac aaacgtatct accgaaccaa
    40441 actgtacagt gacactcccg taaacctctt taaattcctt tttaggcaaa agagcaactg
    40501 tcaatccgtt ggccaaacga gttcgataaa ccttttcttt tacagctgga tagtattttt
    40561 cttcaaaaac aacctttgtc attctattcc ttccataaag taaatcgctt gtagtttcac
    40621 attattagct actctacaaa tagcatcttt gtcaatttgt tcaagctttg caatccaact
    40681 tttaaagtct gctgaagatt ttccaaataa ggcattttga taagcacgtt caatcaatga
    40741 agattgatta tcttgagaaa gtaacaacga ccaacgaatc atttccttgg tctgatttaa
    40801 ctcaaactct gtaaaataac ctttttttaa atcaagcagt tgattattca tcattttacg
    40861 agcctggtta cgattttctc gattgatacc agcatacatc ctcaagaatc cactaaataa
    40921 atcaagctca cttgaaatgg tataagctaa tccagcattt tcacggacat ttgtaaagag
    40981 cttagagtga gcaaatccac caagtaaacc attcattaca atcatgggta aatgttgttc
    41041 atcaccatat ttagaacggt aatgataacc taattccaaa atggattgtc ccacattttt
    41101 ccgaaccata ccttcctgaa ggatattaga ataaggttga caatactgaa ccttcacatc
    41161 tccttttcga cctttaaagc caaatgattc taatacattt tgaatttcaa cctcattaaa
    41221 atcacctagg aaaaagaaat ctattcgatc attggctaaa aattcttgga aacaagaata
    41281 agaactttgt ggagtttcag ctaaaatacg atttcgtaaa tcactatatt ccaattgaag
    41341 acgttcatca tgaaaaaaca atttatccaa ttctttatgt gcaaaataaa aagaatcatc
    41401 catatcagct gctaaacttg ctagcaattg ttttttctca atttcaaata aggccggatc
    41461 aaacccatta tcaactacta cgggtgaaaa aagagtttct tttacaagtt ccaaaatctg
    41521 agaggttagc acgtttttcc tacttaaaaa ctcatcacga acataggtaa atgtcaattc
    41581 tataatgtgg ctttgccctc ttctgaaaca attggttgac atatctgtac cgtatagact
    41641 ggccaagtgt ctcctcaaat cttgagaagt ggggtacatc tgattagcag tctctagcat
    41701 acttgcactc aacatgtgac ctgcaatcgt atcaagggat aatggagcgg taaaacgcac
    41761 ggtaattttg tttgttttaa acttttttga ttggataaaa tgtgttgaaa ttccatgcac
    41821 taactccatg atttacctcc ttatatacag acttctatta tatcacgaaa acctgaaatt
    41881 ttcttgttcc ctgtaacact tttgaaataa aaatcgctta catttctcgc tgtctctctc
    41941 actattttta ggaaaatatg gtataatacc aaagattgag gtgaattatg gaatacaaat
    42001 tatttgaaga atttattacc ctccaagcac tactcaaaga acttggaatt acacatagcg
    42061 gaggagctat taaatcattt ctctctgaac attctgttta ctttaatagg gaattagaga
    42121 gtcgtcgtgg aaaaaaactt cgtattggcg ataaagttga catccctgac atgaacattg
    42181 acatcttgtt gacacaacct acttctgaag aacaagatga ataccaagct gataaagttg
    42241 aaaaagaacg gatcgctaaa cttgtcaaaa agatgaataa gggagttaaa aaagacaaat
    42301 cgaaacctgc ttcatcacct aaaagccaac aagctccacg attccctggt agataatcat
    42361 gtggctacaa catctatctc tcaagacttt tcgtaattac aaagaaacga aaatagactt
    42421 taatcctaaa ttgaatgtct ttttgggacg taatgcacaa ggaaaaacaa acatgttaga
    42481 ggctatctat tttttagcct taacgcgtag tcatcgaact cgaacagata aaaatctcat
    42541 tcattttgat gaggaacaac ttcatctttc aggtctcgtt cagaaaaaaa ctggatccat
    42601 tcccctagaa atcgaactaa cacaaaaagg ccgtgtaaca aaagttaatc acttaaaaca
    42661 ggcacgcctt tcagattatg taggacacat gaatgttgtc ttatttgctc ctgaagattt
    42721 acaactaatt aaaggagcac cttcgattcg acgaaaattc attgatatgg aacttgggca
    42781 aattaagcca atctatttat ctgacttaac caattataac cacatcctaa agcaaagaaa
    42841 cacttaccta aaatcagctc aaaaaataga tgaaacattc ctttctgtgt tagatgatca
    42901 gctagttgat tatggatgtc gtgtaatgaa tcaccgctta gatttcataa aaaaactaga
    42961 atcatttggg cgtaagaaac attttgaact ctctaatcag atcgaagagt tgtcaatatc
    43021 ctatcaatct tctgtcaata taactgacaa acaaaactta tccgaatctt tcaaaattgc
    43081 tttagaaaaa agtagatcca gagatttatt taaaaagaat actggtgtcg gtcctcatcg
    43141 agatgacatt tctttttata taaatgggat ggatgctagt ttcggaagcc aaggtcaaca
    43201 tcgtagtctc gtcctctcga taaaattagc agaaatcgaa ttaatggaaa gcattaccac
    43261 agaatctccg atattactgc ttgacgatgt gatgagtgaa cttgacaaca ctagacagtt
    43321 aaaattatta gaaacgattt ctcaatcaat ccaaaccttt atcacaacaa caagcttaga
    43381 ccatcttcaa aatctgccag aaaatctaag tatcttcact attcaggatg gtaaagctgc
    43441 tgtaaatgga aattgacaac attgtaaaag aactcctgtt ttcactggag ttcttttcat
    43501 tgttttttac atagaataat ttggtgcctc attagtaatt tgcacatcat gaggatggct
    43561 ttctttcaaa ccagcaccag acatttcaat aaattgagca ttatcgtgta gttctttaag
    43621 gttagctgca ccacagtaac ccataccaga gcgaatacca ccaatcattt ggaaaacaat
    43681 atcagctgcc gctcctttat aagcaacacg accttcaatt ccttctggaa cgagcttgtt
    43741 tgcttcattg acagaacctt ggaaataacg gtcgcttgaa cctttcttca tagcagcaat
    43801 tgatcccata ccacggtaag tcttgaattt acgtccttgg aagatttcag tttcgcctgg
    43861 agcttcatca gttccagcaa acatagatcc aagcataaca gcatttccac ctgcagcaag
    43921 tgcttttaca atatctccag aatacttgat cccaccgtca gcaataatcg ttttaccata
    43981 ttcgcgcgca acagctgcag catcgtagat agctgttact tgcggaacac caacaccagc
    44041 aatcacacga gtagtacaga tagaacctgg tccaatacca accttaacaa cgtctacacc
    44101 cgcttcataa agggcacgtg caccttcagc agtagcaata tttccagcaa tcaaagtccg
    44161 atctgggaaa tgagcacgaa tctcggcaat tttacgcaag acacctgcag aatgaccatg
    44221 tgcagtatca ataacaatcg catccgctcc tgcctcaaaa agagcctctg cacgttcaaa
    44281 tgtatctgaa gtaacaccta ctgcacctgc aactagaaga cgaccaaact catctttagc
    44341 cgcatttgga aactcaataa ctttttcaat atctttgata gtgatcaaac cagaaagact
    44401 gccttcttca tcgaccaacg gaagtttttc aatacgatgc tcttgaagaa tactctcagc
    44461 cgttgcaaga tccgtaccca caggagcagt aacaagattt tcactagtca tatggtttga
    44521 aattggttga ttataatctg aaataaaacg aagatctcgg tttgtcaaaa taccaaccaa
    44581 tttacgattt tcaagtgttt caacaactgg aacaccactg atgcggtaac gacccataag
    44641 ctcatctgct tcagcaattg tatgttcagg cgtcaagaag aacggatcaa taataactcc
    44701 attttcagaa cgttttacct tacgaacctc gtctgcttgt tgagcaattg acatgttttt
    44761 atggataact ccgagaccac ctgcacgagc aatagcaatg gccatttgac tctctgtaac
    44821 tgtgtccatg gcagcggtaa taattgggat atttaaagtc aaattatctg ccaatttagt
    44881 tgttaaatct gcatcgttag gcaacacatg actttcagct ggaataagca atacatcatc
    44941 aaaggtaaaa ccttttttca aaaatttagt gtcccaatta gacattcgaa gtttcctctt
    45001 ttctttcttt ttgagcttga ctctttttat attgtatcta tcataccatt ctataaaaat
    45061 ttgtcaaatg ttacaggggt aaataaaaaa ctatcttttc ttcgagatag aaatgatagt
    45121 ttcttgtaaa ataaacttag ttaaagtaat gaagtcccat tgcccctttt acctcagata
    45181 gggtttgacc cgcaacttca cgcgctcttt cactaccttt ttgaatcata ttataaactt
    45241 ctcccatatc cttagcaaat tcaatacggc gctcacgaat aggacccagt tcacgttcta
    45301 atatttcaag tagataacgc ttggtcttca catcaccaag accacctcgt tgataacgtt
    45361 ctttcatatc agcaatttct tgagcatctt ctggacgacc aaaaacatct agataatgga
    45421 aaaccatatt tccctcaatc ttacctggat cctcaacgcg gatatgatct ggatctgtat
    45481 acatactcat tactttttta cgcaaagtat ccgcatcatc agctaaataa ataccattat
    45541 ttagtgattt agacatttta gcatttccat ctaaaccagg caaacgccct gctctctcat
    45601 tttctggata aataccttcc ggctctacca agacatcaca gttatatgca ttgttaaaag
    45661 aacgaacaat ttcacgagtt tgctcaatca ttggtttctg atctgtccca acaggaacat
    45721 aattagcctt gaaagctgtg atatcagctg cttgagcgat tggatagacc aagaatcctg
    45781 tcggaatgct ttctccaaat cctttctgag aaatctctgt cttgactgtt ggatttcgct
    45841 ccaaacgtgc taacgaaact agattcatat aatacataga caactcagcc aactctggaa
    45901 tctggctttg aataaaaata gttgacttat ttggatccaa tccaactgca agataatcca
    45961 aagccacatt tccgatagac tctacaatgg tttgaggatc tttggcatga tctgtcaagg
    46021 cttgttggtc agccaagaac acaaacatat catacttatc ctcttcctgt aataatactc
    46081 gatttttgag acttccaaca taatgtccaa tatgcaattt tcctgttgga cggtctcctg
    46141 ttaaaataat gggtttagtc attttgttct ccttcgatat agtcctttca attatagcat
    46201 ttttttaatg aaataaccgt aatttatcaa aataaatcag ccttgctttt tacagatttg
    46261 tgtaaaggat actgtaaata taatttacac aaaattgaca aataaaaatt tgaatatttc
    46321 tgtattttct agtagaataa atatattaca catataggag aaaaacattg cttacagtat
    46381 ctgatgtttc actacgtttt agtgatcgca aactttttga tgatgtcaat atcaaattta
    46441 cagaaggaaa tacttacgga ttaatcggtg ctaatggtgc cggaaaatca acctttttaa
    46501 aaattttagc tggagatatc gaacctacta ctggtcacat ctctcttggt ccagatgaac
    46561 gtctctctgt tcttcgtcaa aatcactttg actacgaaga tgaacgtgcc attgatgtcg
    46621 ttatcatggg aaatgaaaaa ctttatagca tcatgaaaga gaaagatgct atctacatga
    46681 aggaagattt ctcagacgag gacggggttc gtgctgccga actcgaagga gagtttgccg
    46741 aacttggagg ttgggaagca gagagtgaag cctctcaact acttcaaaac ctaaacattc
    46801 cagaagaatt acactaccaa aacatgagcg aattggccaa cggtgaaaaa gtaaaggttc
    46861 tcctcgccaa agcacttttt ggtaaaccag atgttcttct cttggacgag cctactaacg
    46921 gtttggatat ccaatcgatt acttggttag aagacttctt gattgacttt gataacacag
    46981 ttatcgtagt atcccacgac cgtcacttct taaacaaagt ttgtactcac atggccgacc
    47041 ttgactttgg aaaaatcaaa ctctatgtcg gaaactacga cttctggaag gaatcttctg
    47101 agcttgctgc taaattgcta gcagaccgta atgctaaagc agaagaaaaa attaaacaat
    47161 tgcaagaatt cgttgctcgt ttctctgcca atgcttctaa gtcaaggcag gcaacatcac
    47221 gtaagaaaat gcttgataag attgagctag aagagattgt accatctagt cgtaaatatc
    47281 catttatcaa ctttaaagcg gaacgtgaga ttggtaatga tctcttgaca gtagaaaatc
    47341 taactgtaaa gattgatggt gaaactatct tggataatat tagtttcatc ttgcgtccag
    47401 atgataagac agcacttatt ggacaaaatg atattcaaac gactgcatta attcgtgcaa
    47461 tcatgggaga tattgactat gaaggaactg tcaagtgggg agttacaact agtcaatctt
    47521 acctaccaaa agataactca gctgattttg caggaggaga atcaattctt gactggttgc
    47581 gtcaattcgc aagtaaagaa gatgatgaca atactttcct acgtggtttc ctcggccgta
    47641 tgctcttctc tggagatgaa gttaacaaac ctgtaaatgt cttgtcaggg ggagaaaaag
    47701 ttcgtgtcat gctttcaaaa ctcatgctct taaaatcaaa tgtccttgta cttgatgatc
    47761 caacaaatca cttggacttg gaatctatct caagcttgaa tgatggattg aaaaacttta
    47821 aagaatcaat catctttgcc agccatgacc acgagtttat tcaaactttg gctaaccata
    47881 tcattgtctt atctaaaaat ggcgtcattg accgtatcga tgaaacctac gatgaattcc
    47941 tagaaaatgc agaagtacaa gcaaaagtta aagaactttg gaaagactaa ataaaacttc
    48001 aaaactcagt tgggttaacc aactgagttt tctatcattc tacgaggtaa catgaaatca
    48061 tttttaaaaa catatcgaac ctattttatt tctttcatca ttcctgtagt gattatgtct
    48121 ggagtatatc tatctcaagg tatctactgg aatagcgaca actctcctct attaggagat
    48181 ggctttcatc aatacgttat ttttgatgta gccttacgaa atatcctaca tggaaatagt
    48241 agtctgtttt acacctttac aagtggtcta gggctaaact tctatgccct atctagttat
    48301 tacttgggta gttttctcgc gcctctagtt tacttttttg atctaacgaa tatgccagat
    48361 gctgtctatc tgaccactct cttaaaattt ggattgattg gtctgtcaac cttttttagt
    48421 ttgaataaat tgtttcaatc tatccctcag actttaaaac tagccttatc tacttcctat
    48481 gctctgatga gtttcactgt cagtcaatta gagataaaaa cctggctaga tgtttttatc
    48541 ttgattcctt taattataac tggtttacat ctactgataa ctgaaaagaa actcctattg
    48601 tactttacaa gtctgtcaat cttatttatt caaaattatt attttggata tatgacagta
    48661 ttgtttctta ttttctggta tctctgtcaa atttcgtggg actttaagac tcgaaaatca
    48721 tctgttcttg atttcatagt tatctccttt ttagctggta tggctagttt gattatgact
    48781 cttcccactc tatttgattt acagacacat ggggaaaaat tgactgaagt tacaaagttt
    48841 caaactgaaa gtagctggta tcttgatctc tttgctaagc aattcattgg ttcctttgac
    48901 acaacaaagt atggggccat cccaatgatt tttgttggac tacttccctt tattttgacc
    48961 attttatttt ttacgctgaa atctattaag tttcacgtga aactcatata tgtaatattc
    49021 tttgcatttc taattgcaag cttttacata gaagctcttg acttattttg gcaaggcatg
    49081 catactccaa acatgttttt acatcgctat gcttggattt tctctacctt gttaatttac
    49141 acagcagcag aagtcttaaa gcgtctgaaa gaacttaaag tctggaattt tttagtttcg
    49201 ctttttcttg tagtagcagg atttttagct accatctatc taaaatcgca ttattctttt
    49261 ttaacagatt tgaatattct gcttactctt gaatttttgg ttgtctattc tcttttactc
    49321 cttgcagtta tcaaaaagtt tatatctgtg aatctatttg ccattctaat ctctttattt
    49381 atactggttg aaatgagttt aaatgcttca tctcaaatgg acggaattgc taaggaatgg
    49441 ggatttgctt ctcgaagtgc ttatagtcga gatatcccag ctatggaatc tttctcaaca
    49501 tatattggaa atcaatttac tcgtactgaa aaactacaaa ctcagacagg aaatgacagt
    49561 atgaaattca actacaatgg aatctctcaa ttttcatctg ttcgaaatcg ttcatcaagc
    49621 tctactttag ataaacttgg ttttaaatcc tctgggacta atctcaatct ccgatatgca
    49681 aataatagta ttttggctga tagtttattt ggtatccagt acaatatctc agacagtcct
    49741 attgataagt atggttttaa agatatctat caaaaagata atcttaccct atatgaaaat
    49801 caatactctc ttccgattgc agttgctagt caatctgttt acaatgatgt caagttcaat
    49861 gaacatacct tggataatca ggcctcattt ttaaatcaac ttgctaacgt caattttgat
    49921 tatttttctc caatatctta tgaaaaaaca gaaaaaatag aaaatactaa tgatttgatt
    49981 agtgtcacaa gttcttcaaa tgaagatgca gcaatccagt atcaaattga agttccagaa
    50041 aacagccaag tttatctctc tttcataaac cttcactttt ctaacgataa acaaaagaag
    50101 gttgacatcc ttgtaaatgg tgaaaaaaag acttttacaa ctgataatgt cttctccttc
    50161 tttaatctag gatatactaa agagaaaaaa actttcaata tcaatgttag tttccctgga
    50221 aattcacaag tatcatttga atctcctacc ttctatcgtt tagataccaa aactttcacc
    50281 gaggcaattc aaaaaattaa agaacaacct gtcacagtat caacttctaa aaacaaggtt
    50341 tttgctacat atgatgtcca acaagataca tctattttct tcaccattcc ttatgacaaa
    50401 ggttggtctg cctaccaaga tggtaagaaa atagaaatta aacaagctca aactggattt
    50461 atgaaagttg acattcccaa ggggaaagga actattacac tttccttcat tcccaatggt
    50521 tttattactg gagcaatctg ttcctttact tctctcttac tatttggaat ctataatcac
    50581 agacgaaagt catctaaggc ataaaaaatc gaccagttaa tcggtcgatt tttttaatct
    50641 tcttccattt tcttatgttg ataggctagc atacctaaac cagtaaatag agctagtatg
    50701 acagctagga aaatgacttg ttgatggaga ttgtttgtcg taatcccgaa actgaatagc
    50761 tcattggtaa ccagggatta atagatctaa agaaatcatt tgtcaaagca agtggataag
    50821 tacctgcact tgatgctaac tgtagtaaaa gcaaaataag tgagaagaaa gctcctatac
    50881 ggctattcca cgttgttaaa gtggtcacca tagacatgaa tactaaactt gttaggatga
    50941 tgagaataaa tattctcatc tcatgattag cagttaaacc aataagatga actcctccat
    51001 ataccaaaat tcctgccaaa acagctataa taccatttat ttcagctcga gatttcaacc
    51061 aagcccaacg gctctctgga tgacgtcctg agaaacttta tcaacacctg tagtatatgc
    51121 gtttacacct gatgcaatcg actgactggc aggaactaat ttgctagtaa catctccttg
    51181 tatctctgtt aatccacttg acaatcctat caaagaagta gaagtaatag gtgatacttg
    51241 attagattga ttttttaaag tcgaaagatt agaagattga ttttgtaagt tttctaaact
    51301 tccctgtaaa tcttgtacta aagctacaat tgactgagcc gattgaatac tagcagttaa
    51361 attttgagat acagaatcac ttatctcagt ttgttgctca cttgtcaatg attgataagc
    51421 tgctgtcgat tgaatattgg ataatgtagt tgttttttca gataaatcac ttggtaaagt
    51481 cactaccata tagtaatcgc catcttccaa tcccttcttt ccttcctctt catctacaaa
    51541 atgaaaatcc aaggttttat tttcttttaa attggacacc atgtcttttc ctattgccat
    51601 agtattacca ttataggaag cctctttatc attatttaca actgccacag gtaagtcaga
    51661 caattgccca tatggatccc acattgatga caaaaatatg atattgtaca gatctggaat
    51721 aagagaaatc ccgatcatga caataataaa ggttggtttt ttttaaaatt gctttccatt
    51781 ctttaaactt agtttctcct tttttaaaca tattgtctaa aattttgata taatagatta
    51841 tacattaaaa aatctaaatt acaagataaa aaatctattt ttagacatat agtctaattt
    51901 gtttacaagg aggaaatatg caagaaagta acaaacgctt aaaaacaaag cgaactattg
    51961 aaaatgctat ggtacaatta ctgatggaac agccatttga taaaatttct actgtcaaat
    52021 tagtagaaaa agccggaatt agtcgttcca gcttctatac tcactataag gataagtatg
    52081 atatgattga gcactatcaa agcaagctat tccatacatt tgaatatatt tttcaaaaac
    52141 atgctcatca caaaagagac gctattttag aagtttttga atatctagag tcagaaccac
    52201 ttctggctgc ccttctttct gaaaatggga ctaaagaaat ccaaaatttc ttacgaaata
    52261 aacttcatat catgcttagt acagatttac aaaagcgatt tatgcaactg aatctaaata
    52321 ccaccgaatt agaatatagt agcatctatc taactcatgc actttttggt gtctgccaaa
    52381 cttggattgc acatggaaaa aaagaaagtt ctcaagaaat aacagacttc cttatgaaaa
    52441 tgcttggtga tacaaattga tacaaaaaga ggaacacagc tgtgttccct tttatctata
    52501 ctccgccagt aggactcgaa cctacgacat catgattaac agtcatgcgc tactaccaac
    52561 tgagctatgg cggataaaat agtccgtacg ggattcgaac ccgtgttacc gccgtgaaaa
    52621 ggcggtgtct taaccccttg accaacggac cttctatctg tagcagatat aaccattata
    52681 tcaatttctt gctaattgtc aatcactttt gagatttttt ctctaaaata tcttttaatt
    52741 ttctaatttt taatcttgaa ataggacaac gatggtcttc atagaaaaca atttctaagt
    52801 tttttcgatc aatttctctg atattgccta tatttaccaa aaatgacttg tgaggagaat
    52861 aaaatcgctg agtatgtttg tccttttcct gaatatctgt catggtacca taaaactctt
    52921 ttgcaaaatt cttaccaata atgcgcaatt tatgagatac ccctgttgtt tcaatataca
    52981 aaatatcatg gtaaggaatt tttaaatcat ttcccttgta attgtagtcg aaataatcta
    53041 caacatcttc attttcaagt aacatactct tcgtgtagaa gatattttgc tcaattctct
    53101 tcttaaacat ctcatcattg atatccttat caacaaaatc tagggctgat acctgatatt
    53161 tataggttag agtcgcaaac tctgatcgac tagtgataaa gacgataata gcgtaaggat
    53221 tgtaatgacg aatgagctga gccacttcaa atcccttttt ctcaattcca tgaatatcga
    53281 tatctaggaa ataaagctga tttacttcat cattttcaat gtattcttca aattcacgga
    53341 cttttcccgt tgtcttgtat gatattggaa tattcgattc tttcgaaatt tcatccaata
    53401 ttctctctag tctcacttga tgttcaataa catcttctaa aattaaaact ttcattcaaa
    53461 ttccctctta aatctaatga tttgtctaaa tgtactgcct tccatctctg tttctaaaat
    53521 aatattgttg tacttatcta gtagttcttt cacattattt aatccgactc cgcgatttct
    53581 tcccttagtg gagaatccta aggcaaatag atctcctgaa ggagtcatcg tcattttaca
    53641 tgaattctga atcacaataa ctgtttcagt ttccatctta ataactgcta cttccatctg
    53701 ctttttatag ctatcagccg atccttcgac agcattattc aataaaacgc tcatgatacg
    53761 aaccaaatcc aatagttcaa ttggaagctt ggtaatcgta tcttttactt ccagtgtaaa
    53821 ctctacacca ttatttcgag catagacaat tgactgagca accaaacttc gtaaagctga
    53881 gtcttctatg ttgttcaaat caaagtaagt gtacttatct gaacgcaatt tatgatttgc
    53941 tttgactaaa acttcattgt aaattctgtc aatttcctgt aaattaccac tgtcaattgc
    54001 catctgcatg ctgacaagca ttccagcata atcatgtcga aaaccacgga tttcattata
    54061 cagaccaaca atttcatctg tgtaattctg taaatgtttc tgttcaaatt tcttctgctt
    54121 caaagcaatc tctttctcca tttgaacttt atgagaattc attgcaaaga aggtcaaaag
    54181 gagagagata aagacaatag atgacaaaat acttccaaaa ctattcaaat gtttaatcgt
    54241 acttaccata tctgaaacga aagatacaat atgtagcaat agtaaagcaa aaaatacttt
    54301 tttcaagaaa ggataaaggt agtcctcgtc aaaataggct agttccaaat ggaaatagta
    54361 aatgattttt aatgtaacaa aataggttaa caccgtcaca acgaaaaaga atgggaaatg
    54421 atattgtaaa acaaaattat ctcctgttat agaggagaaa gttacggaca gaaagttatg
    54481 agtgctctca tataaaagag atagtagtaa acttaggaat agtcctctat ccctctcata
    54541 ctgtttcatc catcgaaaat aggaatataa tcccaaagga aataaaaatc tttcaatccc
    54601 taacccatct aaatatagaa tatataaggg aaaatcaaat actatttctg ttagtaatgt
    54661 ataagcacca aaaacgaata attcttttct atttatttga cctttacaaa taaaatggta
    54721 actgtgacta ataattaaaa aatgaacaat aaccgtccca aatccaaata aatccattac
    54781 tctttcccct tatttcatta cttttttctt tgtaaaataa aatcacggaa gaattttgac
    54841 aacctcatct ccccaccttt aatcttttgt aagtcttttt ccttcaaagc tacaaactgt
    54901 tccaatttaa ctgtgttttt cataataaaa tctcctaaaa tgttttttct tgtaagctaa
    54961 cttacaaaaa acattataca aaatggaatt tcgttttaga taaaattctc tcaactgtca
    55021 tttttttctc ccaaagtgta cttttttaag aaaaaagccg ggaaaattcc cagctttgct
    55081 attatattga tcccagcagg attcgaacct gcgaccgttc gcttagaagg cgaatgctct
    55141 atccagctga gctatgagac ctaatacaat tattctacca aaaattcaat taaaagtcaa
    55201 ttttctattt atggtagggg aatccctgct gaatcgtaaa agcgcgatag atttgttcaa
    55261 caagaactag tctcattaac tgatggggta aggttaggcg accaaaactg acagaaagat
    55321 tggctctatt ttttacagat gatgacaatc ctaaacttcc cccaataata aaagtaagag
    55381 tagaaaatcc ttttatagaa gtttcttcta actgcttact aaattcttct gagaagaaag
    55441 ttttcccttc aatggctaac acaataacga aatcacggtc agcaattttt gataaaattc
    55501 tctgaccttc tatttctaaa atcttttgat tttctgattc actggcctta tctggtgttt
    55561 tttcatctga taactcaatc atttcaaact tagcaaatct agaaattcgt tttgaatact
    55621 ctgcgatacc atcttttaaa tacttttctt tcagtttccc aactgttaca actttaattt
    55681 tcatgactct attctaacat attctctatt ttttcacatc ttattcacaa aataaaaaat
    55741 agatttcaat taagaaaatc acagtttcaa aagagttatc cacagtttgt gtaaaacttt
    55801 tgtgtttaag ttataattaa gctagtcagt ttatactttc agtaattcaa acatatggag
    55861 gcaaatatga aacatctaaa aacattttac aaaaaatggt ttcaattatt agtcgttatc
    55921 gtcattagct tttttagtgg agccttgggt agtttttcaa taactcaact aactcaaaaa
    55981 agtagtgtaa acaactctaa caacaatagt actattacac aaactgccta taagaacgaa
    56041 aattcaacaa cacaggctgt taacaaagta aaagatgctg ttgtttctgt tattacttat
    56101 tcggcaaaca gacaaaatag cgtatttggc aatgatgata ctgacacaga ttctcagcga
    56161 atctctagtg aaggatctgg agttatttat aaaaagaatg ataaagaagc ttacatcgtc
    56221 accaacaatc acgttataaa tggcgccagc aaagtagata ttagattgtc agatgggact
    56281 aaagtacctg gagaaattgt cggagctgac actttctctg atattgctgt cgtcaaaatc
    56341 tcttcagaaa aagtgacaac agtagctgag tttggtgatt ctagtaagtt aactgtagga
    56401 gaaactgcta ttgccatcgg tagcccgtta ggttctgaat atgcaaatac tgtcactcaa
    56461 ggtatcgtat ccagtctcaa tagaaatgta tccttaagat cggaagatgg acaagctatt
    56521 tctacaaaag ccatccaaac tgatactgct attaacccag gtaactctgg cggcccactg
    56581 atcaatattc aagggcaggt tatcggaatt acctcaagta aaattgctac aaatggagga
    56641 acatctgtag aaggtcttgg tttcgcaatt cctgcaaatg atgctatcaa tattattgaa
    56701 cagttagaaa aaaacggaaa agtgacgcgt ccagctttgg gaatccagat ggttaattta
    56761 tctaatgtga gtacaagcga catcagaaga ctcaatattc caagtaatgt tacatctggt
    56821 gtagttgttc gttcggtaca aagtaatatg cctgccaatg gtcaccttga aaaatacgat
    56881 gtaattacaa aagtagatga caaagagatt gcttcatcaa cagacttaca aagtgctctt
    56941 tacaaccatt ctatcggaga caccattaag ataacctact atcgtaacgg gaaagaagaa
    57001 actacctcta tcaaacttaa caagagttca ggtgatttag aatcttgatt gacatctatg
    57061 taaagaaagc tttacataag agaaaagatg tgttagtgta gaatcatgga aaaatttgaa
    57121 atgatttcta tcacagatat acaaaaaaat ccctatcaac cccgaaaaga atttgataga
    57181 gaaaaactag atgaactagc acagtctatc aaagaaaatg gggtcattca accgattatt
    57241 gttcgtcaat ctcctgttat tggttatgaa atccttgcag gagagagacg ctatcgggct
    57301 tcacttttag ctggtctacg gtctatccca gctgttgtta aacagatttc agaccaagag
    57361 atgatggtcc agtccattat tgaaaattta cagagagaaa atttaaaccc aatagaagaa
    57421 gcacgcgcct atgaatctct cgtagagaaa ggattcaccc atgctgaaat tgcagataag
    57481 atgggcaagt ctcgtccata tatcagcaac tccattcgtt tactttcctt gccagaacag
    57541 attctctcag aagtagaaaa tggcaaacta tcacaagccc atgcgcgttc cctagttggg
    57601 ttaaataagg aacaacaaga ctatttcttt caacggatta tagaagaaga tatttctgta
    57661 aggaagttag aaactcttct gacagagaaa aaacaaaaga aacagcaaaa aactaatcat
    57721 ttcatacaaa atgaagaaaa acagttaaga aaactactcg gtttagatgt agaaattaaa
    57781 ctatctaaaa aagacagtgg aaaaatcatt atttcttttt caaatcaaga agaatatagt
    57841 agaattatca acagcctgaa ataaggctgt tcttttattt ttttatctca caaggttatc
    57901 cactatgttt ttcgataaaa agcttaataa atcaataatt tcttctttta tccccaacct
    57961 gtggataaag tttggtaaca ttgtggatta tttttcacag cttgtggaaa attcttgcta
    58021 tctatggtaa aatatctcta gtattaaact tttaaatagt aaaggaggag aaaggattga
    58081 aagaaaaaca attttggaat cgtatattag aatttgcaca agaaagactg actcgatcca
    58141 tgtatgattt ctatgctatt caagctgaac tcatcaaggt agaggaaaat gttgccacta
    58201 tatttctacc tcgctctgaa atggaaatgg tctgggaaaa acaactaaaa gatattattg
    58261 tagtagctgg ttttgaaatt tatgacgctg aaataactcc ccactatatt ttcaccaaac
    58321 ctcaagatac gactagctca caagttgaag aagctacaaa tttaactctt tatgactata
    58381 gtccaaagtt agtatctatt ccttattcag atacgggatt aaaagaaaag tatacctttg
    58441 ataattttat tcaaggggat ggaaatgttt gggctgtatc agccgcttta gctgtctctg
    58501 aagatttggc tctgacctat aaccctcttt ttatctatgg aggaccaggc cttggtaaga
    58561 ctcacttatt aaacgctatt ggaaatgaaa ttctaaaaaa tattcctaat gcgcgtgtta
    58621 aatatatccc tgccgaaagc tttattaatg actttcttga tcacctaaga cttggggaaa
    58681 tggaaaagtt taaaaagacc tatcgtagtc ttgatctttt gttaatcgat gatatacagt
    58741 cactcagcgg aaaaaaagtc gcaactcagg aagaattttt caataccttt aacgcccttc
    58801 atgacaagca aaaacagatt gtcctaacga gtgatcgtag tccaaaacat ctagaagggc
    58861 tcgaggagag gcttgtcacg cgttttagtt ggggattgac acaaactatc acaccccctg
    58921 actttgaaac acgtattgcc attttacaaa gtaagacgga acatttaggc tacaatttcc
    58981 aaagtgatac tctagaatac ctagctgggc aatttgattc aaatgttcga gatcttgagg
    59041 gagccatcaa cgacatcact ttaattgcca gagtaaaaaa aatcaaggat atcactattg
    59101 atattgctgc agaagccatt agagcccgca aacaagatgt tagccaaatg ctcgtcatcc
    59161 caattgataa aatccaaact gaagttggta acttttatgg tgttagtatc aaagaaatga
    59221 agggaagtag acgccttcaa aatattgttt tggcccgtca agtagccatg tatttatcta
    59281 gagaactaac agataatagt cttccaaaaa ttgggaagga atttggggga aaagatcata
    59341 ccacagtcat tcatgcccat gccaaaataa aatctttgat tgatcaagac gataatttac
    59401 gtttagaaat tgaatcaatc aaaaagaaaa tcaaataatt tgtggataac ttttagtttt
    59461 ttatcttttt tatccacatt ttttaaacaa gctaaaaaac ttgatatgac ttgtttaaag
    59521 gctgttttcc acagatttca cag
//
LOCUS       DAVLTM010000002        57462 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLTM010000002 DAVLTM010000000
VERSION     DAVLTM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.002"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            76..873
                     /locus_tag="VYN16_000059"
     CDS             76..873
                     /locus_tag="VYN16_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1402657.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGVKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ANSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1021..1143
                     /locus_tag="VYN16_000060"
     CDS             1021..1143
                     /locus_tag="VYN16_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV1402658.1"
                     /translation="MTRDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1165..2205
                     /locus_tag="VYN16_000061"
     CDS             1165..2205
                     /locus_tag="VYN16_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1402659.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2376..3650
                     /locus_tag="VYN16_000062"
     CDS             2376..3650
                     /locus_tag="VYN16_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1402660.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3660..4538
                     /gene="truB"
                     /locus_tag="VYN16_000063"
     CDS             3660..4538
                     /gene="truB"
                     /locus_tag="VYN16_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1402661.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4779..5081
                     /locus_tag="VYN16_000064"
     CDS             4779..5081
                     /locus_tag="VYN16_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1402662.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5189..5827
                     /gene="udk"
                     /locus_tag="VYN16_000065"
     CDS             5189..5827
                     /gene="udk"
                     /locus_tag="VYN16_000065"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1402663.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5955..7295
                     /locus_tag="VYN16_000066"
     CDS             5955..7295
                     /locus_tag="VYN16_000066"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1402664.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEDLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLDSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7273..7485
                     /locus_tag="VYN16_000067"
     CDS             7273..7485
                     /locus_tag="VYN16_000067"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1402665.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7482..8357
                     /locus_tag="VYN16_000068"
     CDS             7482..8357
                     /locus_tag="VYN16_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1402666.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8350..9165
                     /locus_tag="VYN16_000069"
     CDS             8350..9165
                     /locus_tag="VYN16_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1402667.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKGAVERAHSQFKNE"
     gene            9158..9589
                     /locus_tag="VYN16_000070"
     CDS             9158..9589
                     /locus_tag="VYN16_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1402668.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9596..11263
                     /gene="recN"
                     /locus_tag="VYN16_000071"
     CDS             9596..11263
                     /gene="recN"
                     /locus_tag="VYN16_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1402669.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEEFMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLAAARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKVS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11265..11993
                     /locus_tag="VYN16_000072"
     CDS             11265..11993
                     /locus_tag="VYN16_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1402670.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12072..13895
                     /gene="lepA"
                     /locus_tag="VYN16_000073"
     CDS             12072..13895
                     /gene="lepA"
                     /locus_tag="VYN16_000073"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1402671.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14054..14134)
                     /locus_tag="VYN16_000074"
                     /pseudo
     CDS             complement(14054..14134)
                     /locus_tag="VYN16_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14309..14785
                     /locus_tag="VYN16_000075"
     CDS             14309..14785
                     /locus_tag="VYN16_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1402672.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            14823..15128
                     /locus_tag="VYN16_000076"
     CDS             14823..15128
                     /locus_tag="VYN16_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1402673.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            15176..16651
                     /locus_tag="VYN16_000077"
     CDS             15176..16651
                     /locus_tag="VYN16_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1402674.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVLYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            16677..16754
                     /locus_tag="VYN16_000078"
                     /pseudo
     CDS             16677..16754
                     /locus_tag="VYN16_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            16956..23657
                     /locus_tag="VYN16_000079"
     CDS             16956..23657
                     /locus_tag="VYN16_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1402675.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTESRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDSQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLVTVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELSVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYDAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKSGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLYFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENRRWTNWSARPSTNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPSKLVLERYVGPGFEVPTYYSNYQAYESGHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGDDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVATVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(23904..24760)
                     /locus_tag="VYN16_000080"
                     /pseudo
     CDS             complement(23904..24760)
                     /locus_tag="VYN16_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24972..25331)
                     /locus_tag="VYN16_000081"
     CDS             complement(24972..25331)
                     /locus_tag="VYN16_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1402676.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            25423..26379
                     /locus_tag="VYN16_000082"
                     /pseudo
     CDS             25423..26379
                     /locus_tag="VYN16_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            26363..26920
                     /locus_tag="VYN16_000083"
     CDS             26363..26920
                     /locus_tag="VYN16_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1402677.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            26913..27167
                     /locus_tag="VYN16_000084"
     CDS             26913..27167
                     /locus_tag="VYN16_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402678.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            27179..29233
                     /locus_tag="VYN16_000085"
     CDS             27179..29233
                     /locus_tag="VYN16_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1402679.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRNRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            29412..30272
                     /locus_tag="VYN16_000086"
     CDS             29412..30272
                     /locus_tag="VYN16_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1402680.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            30483..31193
                     /gene="ccdA2"
                     /locus_tag="VYN16_000087"
     CDS             30483..31193
                     /gene="ccdA2"
                     /locus_tag="VYN16_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1402681.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLVFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            31213..31779
                     /locus_tag="VYN16_000088"
     CDS             31213..31779
                     /locus_tag="VYN16_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1402682.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            31790..32902
                     /gene="msrB"
                     /locus_tag="VYN16_000089"
     CDS             31790..32902
                     /gene="msrB"
                     /locus_tag="VYN16_000089"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1402683.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            33170..33907
                     /locus_tag="VYN16_000090"
     CDS             33170..33907
                     /locus_tag="VYN16_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1402684.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            33904..35595
                     /locus_tag="VYN16_000091"
     CDS             33904..35595
                     /locus_tag="VYN16_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1402685.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            35711..36274
                     /locus_tag="VYN16_000092"
     CDS             35711..36274
                     /locus_tag="VYN16_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1402686.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            36296..41998
                     /locus_tag="VYN16_000093"
     CDS             36296..41998
                     /locus_tag="VYN16_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1402687.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEV
                     VPDDTKYVDEEVVERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPVVPKVVKKG
                     TKPVEGTAVETREEVIPFATKEQEDGTLKRGTRQVAQEGVNGKKQITETYKTIRAEKT
                     NEAPTVEETVLQAPQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVE
                     IKSIKVALKDNTGTVIKEVTVEENNLNVTLDNLKYYQGYTLSTTMVYNRGEGEETETL
                     EDKEVQLDLKKVEIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKV
                     TRLAVDKIEEVEEDGKTLYKVTAKAPDLVQRNADNTLSEEYVHYFEKQLPKIGNVYYN
                     FNELITDMQKTPNGEFKLGADLNAVNVPTPNKSYVTAKFTGKLYSEGDKHYTIHNLAR
                     PLFAQAENAHIHDINLGNVNINMPWANKTAPLGEMFKKSTIENVKVTGNVVGNNDVTG
                     MVNKLDESDMRNVAFIGNITSVGSAGWWSGGLVSESWRSNTDNSYIDTTIKGNKAKVG
                     GLIAKLNHGADPRDVGARGRLKNSVAKGTIDVRDPQETGGLLHSNWSWGLAENNITMM
                     KVKNGGEILYGSRDAEDDDYFGANWVRNNNAFVNGISEGKQSYSRSSRWKGISEDEAK
                     TRIAKMGITAHEYEITQHLTDKLNRAAFKEDTYKTTQDYKTERELAYRNIEKLQPFYN
                     KEWIINQGNKLTDGSNLMIKEVLSVIGMKNGQFVTDLSDIDKIMIHYADGTKEEKTVT
                     RKADSKVQQIREYSVEGLGDVVYTPNMVEKDRAQLITDIKAKLDSVQLISPEVRNLMD
                     KRGKAHENTDERRNGYIRNLFLEESLDEVKGNLDKLVKALVENEDHQLNGDEAAMKAL
                     VKKVEDNKAKIMLGLAYLNRYYGFKYDEKSMKDIMMFKPDFYGKNVSVLDFLIRVGSR
                     EHNIKGNRTLEAYREVIGGTIGIGELNGFLNYNMRLFTEETDINTWYKKAVSHTNYIV
                     EKQSSNPAFANKKYRLYENLNNGEHGKYILPLLTTKKAHMFLISTYNTLAFSAFEKYG
                     KNTEAEREAFKKEIDLRAQEQINYLDFWSRLAADNVRDRLLKSENMVPSAIWDNQEVP
                     GHGWADRMGHNKNGDYAPVREFYGPTGKWHGYNGTGAYAYIFTNPQNSEAVYYIISSM
                     ISDFGTSAFTHETTHINDRMAYLGGWRHREGTDVEAFAQGMLQSPAVSSPNGDYGALG
                     LNMAYKRENDGNQWYNYDSNKLDSRAKIDHYMKNYNEALMMLDHLEADAVIAKNNGDN
                     NKWFKKMDKKWRENANRNGLVGQPHQWDLLRDLNEEENKKKLTSIDDLVDGNYVTKHN
                     MPGNKHYRAEGFDTAYQTVNMMAGIYGGNTSKSAVGSISFKHNTFRMWGYFGYLDGFV
                     GYASNKYKDAANKENKGLLGDDFIIKKVSDGKFDSLEAWKKEWFKEVKAKGEKGFVAI
                     EIDGKTITNYAELRELFDKAVEADLKAGNSNQTVALKEKVYKQLLQKSDGFVGNLFKA
                     "
     gene            42073..43794
                     /gene="pabB"
                     /locus_tag="VYN16_000094"
     CDS             42073..43794
                     /gene="pabB"
                     /locus_tag="VYN16_000094"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1402688.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            43795..44412
                     /locus_tag="VYN16_000095"
     CDS             43795..44412
                     /locus_tag="VYN16_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1402689.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            44462..45452
                     /locus_tag="VYN16_000096"
                     /pseudo
     CDS             44462..45452
                     /locus_tag="VYN16_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            45587..46546
                     /locus_tag="VYN16_000097"
     CDS             45587..46546
                     /locus_tag="VYN16_000097"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1402690.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            46644..47483
                     /locus_tag="VYN16_000098"
     CDS             46644..47483
                     /locus_tag="VYN16_000098"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1402691.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(47531..47701)
                     /locus_tag="VYN16_000099"
     CDS             complement(47531..47701)
                     /locus_tag="VYN16_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1402692.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            47822..48706
                     /gene="miaA"
                     /locus_tag="VYN16_000100"
     CDS             47822..48706
                     /gene="miaA"
                     /locus_tag="VYN16_000100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1402693.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPYEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            48699..49937
                     /gene="hflX"
                     /locus_tag="VYN16_000101"
     CDS             48699..49937
                     /gene="hflX"
                     /locus_tag="VYN16_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1402694.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            49930..50553
                     /locus_tag="VYN16_000102"
     CDS             49930..50553
                     /locus_tag="VYN16_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1402695.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            50568..51497
                     /locus_tag="VYN16_000103"
     CDS             50568..51497
                     /locus_tag="VYN16_000103"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1402696.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            51518..52273
                     /locus_tag="VYN16_000104"
     CDS             51518..52273
                     /locus_tag="VYN16_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1402697.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(52327..53235)
                     /locus_tag="VYN16_000105"
     CDS             complement(52327..53235)
                     /locus_tag="VYN16_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1402698.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            53299..53568
                     /locus_tag="VYN16_000106"
     CDS             53299..53568
                     /locus_tag="VYN16_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1402699.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            53568..53948
                     /locus_tag="VYN16_000107"
     CDS             53568..53948
                     /locus_tag="VYN16_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1402700.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNKEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            54037..54264
                     /locus_tag="VYN16_000108"
     CDS             54037..54264
                     /locus_tag="VYN16_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402701.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(54304..55029)
                     /locus_tag="VYN16_000109"
     CDS             complement(54304..55029)
                     /locus_tag="VYN16_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1402702.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            55191..57032
                     /gene="typA"
                     /locus_tag="VYN16_000110"
     CDS             55191..57032
                     /gene="typA"
                     /locus_tag="VYN16_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1402703.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            57052..57306
                     /locus_tag="VYN16_000111"
     CDS             57052..57306
                     /locus_tag="VYN16_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1402704.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        17461 a        10662 c        13059 g        16280 t
ORIGIN      
        1 tgaaatacag aaaacgcttc ctaaaaccta aagtttgtga tataattatc aagaaaaaag
       61 atttaggagt ttattatgat ttcttcagaa tttatctcaa agattgaatt tgcttgcaat
      121 aagaaagaaa gtctttatag tcaaagcaaa tttaagtatg cgattcgttc gatgttcgca
      181 ggtgcatttt taaccttcag tactgctgca ggtgcagttg gggctgactt gattaataaa
      241 attgcaccag gtagtggacg cttcctcttt ccattcgttt ttgcttgggg cttggcctac
      301 attgtttttt tgaatgccga gttggtcact tcaaacatga tgttcttgac tgctggtagt
      361 ttcttaaaaa aaatctcttg gagaaaaaca gctgagattt tactatactg taccttgttc
      421 aaccttatcg gagccttgat agcagggtgg ggctttgctc attcggcagc ctatgcgaat
      481 ctgacacacg atagtttcat ctcaggtgtt gttgagatga agttaggccg ctcaaatgaa
      541 ttggtcttgc ttgaggcgat tttggcaaat attttcgtaa atattgcgat tctgtcattt
      601 attttggtca aagatggtgg tgtcaaactt tggcttgtgt tgtcagctat ttacatgttt
      661 gtattcttaa caaacgagca cattgcggcg aactttgctt ctttcgcgat tgtgaaattc
      721 agtgttgctg cgaattcaat tgccaacttc ggtgttggaa atatgcttcg ccactggggt
      781 gtgactttca tcggaaactt tatcggagga ggcctcttga tgggtcttcc atatgccttc
      841 ctcaataaaa acgaagatac ttatgtagat taagaaaatg agcacgattg agtcgtgctt
      901 ttttcatttt caaaataagg taatagctat ttcttatatc aaaatataga aaactgatat
      961 ttgtaaacta taactcaagg tgctacaata tccttaataa aataatatgg aggtcacctt
     1021 atgactcgtg attttaaatt tgaaactcta caactacatg ctggtcaagt tgtggctcca
     1081 gctactaagt ctcgtgcagt gccgatttat caaacaacat tttttgtttt tgatgacacg
     1141 taggaaggtg ccgatctgtt tgccttgagg aaaccaggga acatttatac tcgtatcacc
     1201 aatcctacaa cagctgccct tgaaggtggt gttgaagcgc tagcaacagc atcaggtatg
     1261 actgcagtga cttatactat tttggcgatt gcccatgctg gtgaccatgt agtggctgct
     1321 tcgactattt acggtggaac cttcaatctt ttgaaagaac cccttcctcg ttatggtatc
     1381 acaacaacct ttttcgatat tgataatttg gaggaagtag aagcagctat caaagacaat
     1441 accaagcttg tcttgattga aaccttgggt aaccccttga ttaatattcc agacctggaa
     1501 aaactggcag agattgctca taaacatcaa atcccacttg tgtcagacaa tacttttgca
     1561 acaccttatt tgattaacgt cttctctcat ggcgttgaca ttgccattca ctctgtgact
     1621 aagtttatcg gtgggcatgg tacaactatt ggaggaataa ttgtcgatag tggtcgtttt
     1681 gactggacgg cttcagggaa attccctcaa tttgttgacg agggtccaag ctgccacaat
     1741 ttgagctata ctcgtgatgt gggtgcagca gcctttatta tagctgttcg agttcaattg
     1801 cttcgtgata caggtgcagc cttgtcacca ttcaatgctt tcctcttgct acaaagactt
     1861 gaaacctctt cacttcgtgt ggaacgccat gtacaaaatg ctgagacaat tgttgatttt
     1921 cttgtcaacc atcctaaggt agaaaaggta aattatccaa aacttgcaga tagtccttat
     1981 catgccttgg ctgagaaata cttgccaaaa ggtgtcggtt caatctttac cttccacgtc
     2041 aaaggtggcg aggaagaagc acgcaaggtc attgataatt tagaaatctt ttctgacctt
     2101 gcaaacgcgg cagatgctaa atcgcttgtt gtccatccag caacaaccac tcacggtcaa
     2161 ttgtcagaaa aagacctaga agcagcaggt gtcacaccaa actaaattcg tttgtcaatc
     2221 ggtcttgaaa atgtagaaga tttgattgaa gacttgcgct tggccttgga aaaaatttaa
     2281 agtaaaagaa gataaacagt gggcttcgac tcactgtttt tgattttccc tcaggcatga
     2341 tataatggtt aaagaagtct agaaagagga acgatatgaa cgaaatcaaa tgtcccaact
     2401 gtggggaagt ctttacagta aatgagagtc agtatgccga actcttgtcc caagtgagaa
     2461 cggcagagtt tgataaggaa ctacacgata ggatgaagca ggaactggcc ttggctgagc
     2521 aaaaggccat gaatgagcaa cagactaaac tggctcagaa ggatcaagaa attgcgcaat
     2581 tacagagtca gatccaaaac tttgatacag aaaaagaatt ggccaagaaa gaggttgaac
     2641 agacaagcca tgaggctctc ttggctaagg acaaggaagt acagctctta gaaaatcagt
     2701 tggctacctt gcgtttggag catgaaaatc aactacaaaa gaccctttct gacctagaaa
     2761 aagaacggga tcaggttaaa aaccaactac ttttgcagga aaaggaaaat gaattatctt
     2821 tggcttctgt taagcaaaac tacgaagccc agctcaaggc agctagtgaa caagtcgagt
     2881 tttataagaa ttttaaggct caacaatcta caaaagcgat tggggaaagc ctagaacagt
     2941 atgcagagag tgagtttaac aaggttcgta gtttcgcctt tccaaatgct tactttgaga
     3001 aggataacaa ggtctcttcg cgtgggtcta aaggggactt tatcttccgt gagtgtgatg
     3061 aaaatggagt tgaaatcatt tctatcatgt ttgagatgaa aaacgaagcg gacggaacag
     3121 agaagaagca caagaatgca gatttttaca aggaattgga caaggaccgt cgggagaaga
     3181 actgtgagta tgccgttttg gtgaccatgc ttgaggctga taatgactac tttaacacag
     3241 ggattgttga cgtcagtcac gagtatgaaa aaatgtatgt tgttcgtcct caattcttta
     3301 tccaattgat tggtctctta cgtaatgcgg cgctaaattc cctaaaatac aagcaggagt
     3361 tggccttggt tcgcgagcaa aatattgaca ttacgcattt tgaggaagac ttggatgcct
     3421 ttaagctagc ttttgctaag aactataatt cagcttcgac taactttgga aaagctattg
     3481 atgaaatcga caaggccatc aaacgcatgg aagaggttaa gaaattcctg actacatctg
     3541 aaaaccaact ccgtctagct aacaacaaat tggaagatgt ctctgttaaa aaattgaccc
     3601 ggaaaaatcc aacaatgaaa gcgaagttcg aagcactgaa gggggagtag aaagcaaaaa
     3661 tgaacggtat tatcaactta aaaaaagaag caggaatgac ctcgcatgat gcggttttta
     3721 aactgcgtaa gattttggga accaagaaaa ttggtcatgg cggaaccttg gatccggatg
     3781 tggtgggtgt tttgccgatt gcggttggca aggcgacacg catggtcgag tttatgcagg
     3841 acgagggtaa gatctatgag ggggaaatca ctctgggtta ttccacgacg actgaggatg
     3901 ctagtgggga agtggtcgca gaaacccctg ttttgtctcc cttggatgaa aagcttgttg
     3961 atgaagcgat tgctagcttg actgggccta ttacccagat tccccctatg tattcggcag
     4021 ttaaggttaa tggtcgcaag ctctatgagt atgcgcgtgc tggtcaggaa gtggagcgtc
     4081 cagaacgtca ggtgaccatt tatcaatttg agcgaacaag tccgatttct tatgatggcc
     4141 aacttgcccg attcactttt cgtgtaaaat gcagtaaagg gacttacatc cgtactttgt
     4201 cagttgattt gggtgaaaag cttggttatg cggctcatat gtcccatttg actcgtacta
     4261 gtgctgctgg cttacaatta gaagacgctc ttgccttgga ggaaattgct gaaaaagtag
     4321 aggctgggca attagatttt ctccatcctt tagagattgg gacaggtgac cttgtcaaag
     4381 ttttcctaac tccagaagag gctacagaag ttcgctttgg tcgttttatt gagctagacc
     4441 aaacggacaa agaactggct gcctttgaag atgataaatt gttagccatt ctagaaaaac
     4501 ggggcaatct ctataagcca aggaaggttt ttagctagat cgtttaggaa taaaaatcgg
     4561 gtgatagata acaattgctt gataaaaccc catactaata gtagaatggt tttgggaatt
     4621 ataatattcc aattgttgcg agttgtaggt actcaaataa tctatataga aatttagagg
     4681 tgtgaaatga agcaatttaa aattctttca gataaatatt tagagtccat tacaggttct
     4741 ggtgggaact taggcccagg atttggtgtg ataattccat gatgcgaaat gagtttcgag
     4801 aaagggtgga gcaacttctt caacaaaaag aaataaatga aaatagtgag ttgagtcacc
     4861 tgtttcgtct tgctatacaa aatttagaca gaaatgaaaa ataccaatcg gtcatggcca
     4921 atttgagtca agggttgtca ctttacctca tgacgcatca ttaccaggca cctaagtctg
     4981 tcattgattt tggtttatgg attgccaaag ctcctagcca ggaaagaggg agactggctt
     5041 tcttgcagat gcttgctcaa accctacagg gctttcgtta aaactgtcga acatcagagg
     5101 gaagactctg gtgttttttg ctgatgagac tataaatttt gtaaaaaatg tgatagaata
     5161 gacgacggat aaaataacgg aggatagcat gcaaaataga ccaatcatta ttggagtgac
     5221 aggtggttct ggtggtggta aaaccagtgt ttcaagagcc attttatcgc attttcctga
     5281 tgaaaagatt tccatgattg agcatgattc atactacaag gatcagtctc atttgacctt
     5341 tgaagagcgt gtcaaaacca actatgacca tccttttgcc tttgatacag acttgatgat
     5401 cgagcagatt aaggaattgt tggcagggcg tccggtggac atcccgacct acgactatac
     5461 agagcataca cggagtagca agacctatcg tcaggagcct caagatgtct ttatcgttga
     5521 gggcattttg gtcttggagg acaagcgtct gcgcgatttg atggatatca agatttttgt
     5581 ggatacggat gacgatgtgc gcattattcg tcgtatcaag cgtgatatgg aggagcgtgg
     5641 ccgtagcctt gatagcgtta ttaaccagta cttaggtgtg gtcaaaccaa tgtaccacca
     5701 gtttatcgag tcaactaagc gttatgctga tatcgtcatt cctgaagggg ttagcaatac
     5761 cgtggctatc gacctgttga cgaccaagat tgcaaagatt ttggaagaag ctcgaaacag
     5821 caaataatca catgtggagg ccttgcctcc tttttctatt tttccctcat aatggtacat
     5881 aaatggagat ttttaggcat atttttggta taataatacc catgaaagag caagtaaatg
     5941 aatagtaggt ggagatggaa aagtatttat cggtaacaac tttgaccaag tatctgaaaa
     6001 tgaaattcga taaagaccca tacttggaac gggtctattt aactggtcaa gtttccaact
     6061 ttcgtaaacg acctactcac caatatttct ccctaaagga tgaccatgca gttattcaag
     6121 cgaccatctg gtctgggatt tatcagaaat tagggtttga cctggaagaa ggaatgaaga
     6181 tcaatgtgat tgggcgtgta caggtctatg aaccaagtgg tagctactcc atcatcattg
     6241 aaaaagctga gcctgatggg gttggggcgc ttgcgattca gtttgaacaa cttaagaaaa
     6301 aattgacaga agaagacctg tttcaagaac gcttcaagca agctctgccc caattttcta
     6361 agagaattgg tgtagtaacc agccgtagtg gagccgttat tcgagatatt atcacgaccg
     6421 tcagcaggcg attcccaggt gttgacatcc ttctttatcc gaccaaggtt caaggtgaag
     6481 gggctgcgga ggaaattgct agaaatattg cgcgtgctaa tcaacgggac gatttggatt
     6541 tgctcattat tggtcgtgga ggtggttcta ttgaggatct ctgggccttt aacgaagaaa
     6601 ttgtggtacg agctattttt gaatctcgtt tgccagttat ctctagtgtg gggcatgaga
     6661 cggatgtgac cttggcagat tttgtggcag atcgacgcgc tgcaacgcca acagcggcgg
     6721 ctgaactggc aacacctgtg accaagttgg atgtattaac tcatttgcaa aatcaggaaa
     6781 aacggatggc aacggcagtc cgaaatgttc tatctaagaa acaagaggct ttgaaaaaat
     6841 gcagtcagtc tgttatcttt agacaacctg agcgcttgta tgacggttat ttgcaacgct
     6901 tggaccaact gcaactgcgt ttgaaacaaa gtttgcgaac tcggatttct gataacaaac
     6961 aattagttca agcaagaact catcaattag tacaattatc acctgttacc aaaatccaac
     7021 gctatcaaga ccgtttagga cagttggaca agctcttaga tagccaaatg gcgttagttt
     7081 atgacgccaa ggttgctgag gccaagcgac tttcggaagc tttgctcatg ttggatacta
     7141 gccgaatcgt tgcgcgtggt tatgctattg tcaaaaaaga agagtccgtt gtagattcgg
     7201 ttgagagttt gaagaaaaaa gaccaagtaa cgcttttgat gcgagatggt caagtagaat
     7261 tagaggttaa agatgtcaaa acaaaagaaa tttgaggaaa atctagcaga actggaaacc
     7321 attgtccaaa gtttggaaaa tggtgaaatt gctctggaag atgcgattac tgcctttcaa
     7381 aagggcatgg tcttgtcaaa agagctccaa gctacgctgg acaaggctga aaagaccttg
     7441 gtcaaggtca tgcaagaaga cggaacagaa agtgattttg aatgaaaaag caagaaaaat
     7501 tagctcttgt cgagtcggct ttggaagatt tttatggaga ccagcagttt gcctctagtt
     7561 tacgggagtc tgttctctat tctattcatg ctggtggcaa gcgtattcgg ccttttctct
     7621 tgttagaagt tctggaagcc ttgcaggtta ccatcaaacc tgctcacgcg caggtagcta
     7681 ctgccttgga gatgattcat acagggagct tgattcacga tgaccttcct gctatggatg
     7741 atgacgatta tcgaagaggg cggttaacca atcacaagaa attcggtgaa gctatggcca
     7801 ttttggctgg agatgcctta ttcttagact catatgcctt gattgcgcag gcagatttgc
     7861 caagtcagat taaggtggac ttgattgcca acttatccct tgcttcaggt agtctgggta
     7921 tggtggcagg gcaagttttg gatatggagg gcgaacacca gcacttgtct ttggaagaac
     7981 ttcagactat tcatgccaat aagactggga agttactagc ctatcccttc caagcggcag
     8041 ctattatagc tgaattgtca cctgaaatgc aggtgaagct gaaaactgtg ggtgaattga
     8101 ttggacttgc ttttcaagtc agagatgatg tactggatgt gacagctagt tttgaggaaa
     8161 tcggcaagac acctcaaaag gatctgcagg cagaaaaatc aacctatcct gccctgttgg
     8221 gcttggaaga gtccattgcc ttttgtaacc aaaccctgga tcaagctaat gaaaaattgg
     8281 aagaaattgc ccagcagctt ccctttgaaa cagaatcgat tgtaagtgta gtagaaagtt
     8341 tgagaatcaa tggctaagga aagagtggat gtactagctt ataaacaggg gttgtttgaa
     8401 acgagagagc aggccaagcg aggtgtgatg gctggcctag tcgtagcagt ccttaatgga
     8461 gaacggtttg acaagccagg agagaaaatt ccagatgata ccgaattaaa actcaagggg
     8521 gagaaactca agtatgtcag ccgtggtggt ttgaaactgg aaaaggcctt gcaggtcttt
     8581 gatttgtcgg tggatggcgc gactacgatt gatatcgggg cctctactgg aggttttacc
     8641 gatgtcatgc tacagaatag tgccaagttg gtctttgcag tcgatgttgg taccaatcag
     8701 ttggcttgga aattacgcca agacccacga gttgtcagca tggagcagtt caatttccgc
     8761 tatgctgaaa agactgattt cgagcaggag ccgagctttg ccagtattga tgtgagtttc
     8821 atttccctta gtctgatttt gccagccttg caccgtgtct tggctgatca aggtcaggtg
     8881 gtagcacttg tcaaacctca gtttgaggca ggacgtgagc agattgggaa aaatggaatt
     8941 attcgagatg ctaagattca tcagaatgtc cttgaatctg taacagctat ggcagtagag
     9001 gcaggttttt cagtccttgg cttggacttt tctcccatcc aaggtggaca tggaaatatt
     9061 gaatttttag cgtatttgaa aaaagaaaag tcagcaagca atcagattct tgctgagatt
     9121 aaaggagcag tagagagggc gcatagtcaa tttaaaaatg aataaaaaag aaagacttga
     9181 aaaaattaga agattggtga cagattatca aatcggcacg caagaagaaa ttgtagaaca
     9241 tttgaaagaa gcaggtatca ctgccactca ggcgacggta tcccgagata tcaaagagtt
     9301 aggtattgtc aaaattcctt tgagagacaa cacctatgtc tacgaattac caaaatcaat
     9361 cgtaaaaagt ctgcaactgg ctgaagacaa tatcgaatcg gctgaattga tggataagat
     9421 gatcaatctc caagttattc caggaaatac ggcttttgta aaagctcagt taatcgagac
     9481 ttttgcagac aagattttta gctgtttgac tgatgatagc tcgattttag tcattgccag
     9541 aagtggaagt ctagcagagg aaatctttga acaagtaaaa aattggtagg tctatatgtt
     9601 acttgaaatt tcgataaaaa actttgccat tattgaggct atttccctca attttgaaaa
     9661 ggggatgact gtcctgactg gtgaaacggg tgcagggaag tcgattatca ttgatgccat
     9721 gaatatgatg ttgggagctc gtgcgacgac agatgttatt cgtcatggtg caccaaaggc
     9781 agagattgag gggcttttct cagttgagaa tagtcgtctt ttacaggaaa tttttgatga
     9841 gcaaggttta gaattaggtg atgaaattat catccgtcgg gaaatcttgc aaaatggtcg
     9901 tagtatcagc cgtgtaaatg gtcagatggt taatctgtct gttttgcgag ctattgggca
     9961 acatcttgta gatattcatg gtcagcatga ccatgaggag ttcatgcgtc cccaactgca
    10021 tatccagatg ttggatgaat ttggcgatgc cgctttttgg gacttgaaag aaacctatca
    10081 aacgagtttt gatgcctatc ggaaaatgcg caagcaggtt ctggaagtca agaaaaacca
    10141 acaggaacac aaggcacgta tcgaaatgtt ggaatttcaa atggcagaga ttgaggcagc
    10201 aaacttgcag gctggagaag acttggatct caatcaagag cgagataaac tcctcaacca
    10261 caaaaatatt gcggatacac tgaccaatgc ctacagtatg ttggacaatg aagatttttc
    10321 aagtctggcc aatgttcgtt cagccatgaa tgacatggaa agtgtcgaag agtatgaccc
    10381 tgaataccgt gaaatttcaa gctctctgtc tgagacctac tatgttttag aagacattag
    10441 caaacgtttg gaagctatca ttgaggacct tgattttgat ggcaatcgcc tcatgcaggt
    10501 tgagaatcgt ttggacctcc ttcatactat tactcgtaag tatggtggga ctgttgatga
    10561 tgttttgctt tattttgcca agattacgga agaatacaat ctcttgacag gcaataatct
    10621 ttcgtctgag gacatggaag cagagcttaa gaagttggaa gtcaatcttg tcaatttggc
    10681 aggccaactt gctgctgctc gtcataattt ggctcagcaa ctcgaagctg agattaaaca
    10741 agaactgcaa gatctttata tggaaaaagc ccagtttcag gttcgtttta gtaagggaaa
    10801 attcagtcgt gagggaaatg aaatggttga gttttatatt tcaaccaacc ctggagaaga
    10861 ctttaaaccc ttggttaagg ttgcttctgg aggggaatta tctcgtctca tgttagccat
    10921 taagtctgcc ttttcacgta aagaaggcaa gactagcatt gtctttgatg aggtggatac
    10981 gggagtttca ggtcgtgttg ctcaagctat tgctcagaaa attcataaaa ttggtcagca
    11041 tggtcaggtt ttggctatct cccatttgcc acaagtaatt gcgattgcag attatcaatt
    11101 ctttattgag aaggttagta atgaccattc aacggtttcg actgttcgtc tcttgacggt
    11161 cgaagagcga gtggaggaag ttgccaagat gttggcaggt gatgatgtga cagaagcagc
    11221 cctgacgcaa gccagagaat tgttgagaaa cagggagaaa taagatgaca gattattatg
    11281 taattggaga tgttcacgga aaagctggga tgctggaaga ccttctcaaa acctgggatg
    11341 gtcagaccca gttgctcttt ctaggtgatt tgattgacag aggtgaggat agtcaccgtg
    11401 ttcttgaaat ggtcaaggac ttggtggaca atcaaggggc tatctgtttg tctggaaacc
    11461 acgagtatat gtttttgact tggctagatg acccagaaga aagttatgac cattatcgtc
    11521 gcaatggtgg agatacaacc attaactcta tcctaggtcg tcccttggat gcaccagttg
    11581 atggagtaga agatgccaag cgggttgccg ctgaagcgtc agacttggtc gaattcattc
    11641 gtcaaatgcc atttgtagta gagacagaca agtatatatt tgttcacgca ggtattgatt
    11701 tgactttgga cgactggcat gaaacaacag attataaaaa agtatggctt agaaaaccat
    11761 tccacggagc tgaaaatcat actggaaaaa ccattgtctt cggtcataca ccggtctatg
    11821 gtttgttaca gcaagaacgc ggtacatctg agctttggac tacagaagat ggcaagattg
    11881 gaatggatgg aggagctgtt tatggtggtg tccttcacgg gattgtcttt acagaccaag
    11941 gaatgacaga acaccacttt atcgaaaatg acggctttgt cgctgaagat tagtactcct
    12001 agcagggtat ggtcttgtca aaatatcaaa aacaatttat aataaataga taccctgaaa
    12061 ggaagagaat catgaactta gaagaattga aaaaacgaca ggggaagatc cgtaacttct
    12121 ctattatcgc ccatattgac cacgggaaat caactctagc agaccgcatt ttggaaaaaa
    12181 cagagacggt ttcaagtcgt gaaatgcagg cccagctttt ggatagcatg gatctagagc
    12241 gggaacgtgg aattactatt aagttgaatg ccatcgagtt gaattacact gcaaaagatg
    12301 gggaaactta tattttccac ttgattgaca caccagggca cgttgacttt acctatgaag
    12361 tttcacgttc gctagctgcc tgtgagggtg ctattttggt ggtcgatgcg gctcaaggaa
    12421 ttgaggctca aactcttgcc aacgtttatc tggctttgga taatgatttg gaaatcatgc
    12481 ctatcattaa taaaattgac ctgccggctg cagatccgga gcgcgtgcgt acagagattg
    12541 aagatgtgat tggtttggat gccagtgaag cagttttggc ttctgccaag gctggtattg
    12601 ggattgaaga aatcctcgag caaattgtag aaaaagtacc agcaccaacg ggtgatgtga
    12661 cggcgccact taaggccttg attttcgact ctgtttacga tgcttaccgt ggggttatcc
    12721 tccaagtacg tgtcatggac ggagtggtca aacctggtga taagattcag ctcatgagca
    12781 atagtaagac ctttgatgtg gccgaagtcg gtatttttac accaaaagcg gttggtcgtg
    12841 atttccttgc gactggtgat gttggttaca ttgcggcgtc tatcaagacg gttcaggata
    12901 ctcgtgtggg tgataccgtt accttggcaa ccaatcctgc ggcagaacca ttacatggtt
    12961 ataagcagat gaatcctatg gtctttgcgg gtctctaccc aatcgaatca aacaagtaca
    13021 atgacctgcg tgaagcgctt gaaaaattgc aactgaatga tgctagtctt cagtttgaac
    13081 cagaaacatc tcaggcactt ggatttggtt tccgttgtgg atttcttgga cttctccata
    13141 tggatgttat ccaggaacgt ttagagcgtg agttcaatat tgatctcatc atgacagctc
    13201 cgtctgttat ttacaaagtt aatttgaccg acggtgagtc tatggatgtg tctaacccat
    13261 ctgagtttcc agacccaact aagattgcga ccattgaaga gccttatgtc aaggcgcaaa
    13321 tcatggtacc acaggagttc gtcggagcag taatggagct agctcagcgt aagcgtgggg
    13381 actttgtgac tatggactat attgatgata accgtgtcaa tgttatctat caaattcctc
    13441 ttgctgaaat tgtctttgac ttctttgata aacttaagtc ttcgacacgt ggttatgcaa
    13501 gctttgacta cgaattgtca gaatatcgcc catctaagct ggtgaaaatg gatattcttc
    13561 tcaatggaga taaggtggat gccctcagct ttatcgttca caaggacttt gcctacgaac
    13621 gtgggaaact catcgttgat aaactcaaga aaatcatccc tcgccaacaa tttgaagttc
    13681 caatccaagc ggctattgga cacaagattg tcgctcgtac tgatatcaag gcccttcgta
    13741 agaacgtact tgctaaatgt tatggtggtg acgtttctcg taagcgtaaa ctccttgaaa
    13801 aacaaaaagc tggtaagaaa cgcatgaaat ccatcggatc agttgaagtt ccgcaagaag
    13861 ccttcctcag cgtcttgagt atggatgaag aataagttat cagaaatgcc tttccttgtt
    13921 cacaggaaat ttatataaga atcaatagta aagcaggctt agatggtctg cttttttagt
    13981 ttttatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttactg tatgtatggt
    14041 tactgacttc gtcagtttca tctacaacct caaaactgtg ttttgagtaa cctgcggcta
    14101 gcttcctagt ttgctctttg attttcattg agtattagaa cagaaaaaat gcttggagta
    14161 tttgtttgtg tgtttatttt tatataacaa actataaaca aaacaaaaat ataaaaaaag
    14221 agacaaaaaa gaacagaaag taattgacaa cggtttcata tagtgttata cttatggcat
    14281 aaagttaatg gaaagaaggg aaaaacttat ggtattagat tatttctttg acaaaaacct
    14341 tgtgttttgc ttagaagcgg ataatcaaga acaactcttt gatcaggttg caactttatt
    14401 ggaagaacga gaaattgtca ctccaactta tcgtgaagct ttgatcacgc gtgaaaagtc
    14461 atttccaact ggtttagata tggaatttct aggaaaggac ttgccaaatg tagcgattcc
    14521 tcatacagat attgttcata atctagctga gaaagtggtg gttgttcgat tagaaaaacc
    14581 agtaactttt cacaatatga tagctcctga taaggaagta gaagtatcct tgctcttctt
    14641 tatcatcaat aactcaagtt caagtcaaac aaatattctg gctcagttga tggacttttt
    14701 cacaggaaat ggacatcttg aagacctatc aaaaatttcc gaaccagaaa aactttatgc
    14761 ttacattgct gaagcaaccg cttaatcttg tctattaaaa aataaaatcg gaggaaatcc
    14821 aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
    14881 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg
    14941 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
    15001 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattccgta
    15061 tcccagcaat gagcgctcca gtatttgaca atattgaagc agctattaaa gaacatggat
    15121 taagctaatt ctaatttgtt aacattcata taacataaaa aagggaggaa ctgttatgga
    15181 tgcaatcttt gacctaatcg gaaaggtttt caatcccatc ttagaaatgg gtggacctgt
    15241 catcatgtta atcattttga cagtattggc tttacttttt ggagtgaaat tctccaaagc
    15301 gcttgaaggt ggtatcaaac ttgccatcgc tcttacaggt atcggtgcta tcatcggtat
    15361 gctaaatggt gctttctcag catcacttgc aaaattcgtt gaaaacactg gtatacaatt
    15421 gagtattacc gacgttggtt gggcaccact tgcaacaatc acttggggtt ctgcttggac
    15481 actatacttc ttgctcatca tgttgattgt caacgtagtg atgcttgcta tgaagaaaac
    15541 cgatacactt gatgtcgata tctttgatat ctggcacttg tctatcacag gtctcttgat
    15601 taaatggtat gctgataaca atggtgtgag tcaaggggtt tcactcttta ttgctacagc
    15661 agctatcgtc cttgtcggtg tgttgaaaat tatcaactct gacttgatga aacctacatt
    15721 tgatgacctt cttaacgccc caagttcatc accaatgaca tcaactcaca tgaactacat
    15781 gatgaaccca gttatcatgg ttttggataa gatttttgaa aaattcttcc caggccttga
    15841 taaatatgac tttgatgctg ctaaattgaa caagaaaatc ggtttctggg gatctaaatt
    15901 cttcatcggt ttcatccttg gtatcgttat cggtattatg ggaactccac atccaattgc
    15961 aggtgttgca gatgcagata aatggcgtct tgttatcaaa ggatggttgt ctcttggttt
    16021 gactgccggt gtatctttgg aactcttctc acttatcggt tcatggttca tcgcagccgt
    16081 agaaccacta tcacaaggta ttacaaacgt tgctactaaa cgtcttcaag gacgtaaatt
    16141 caatatcggt cttgactggc cattcatcgc tggtcgtgct gaaatctggg cttgtgccaa
    16201 cgtacttgca ccaatcatgt tgattgaagc agtgcttctt tcaaaagttg gaaatggtat
    16261 cttgccactt gcaggtatca tcgctatggg tgttactcca gctctcttgg ttgtaactcg
    16321 tggtaaattg ctccgtatga ttatcttcgg aacactcttg ttgccactct tccttctttc
    16381 aggtacactt attgcaccat ttgcaacaga acttgctaaa ggtgtaggtg ccttcccaga
    16441 aggtgtgagc caaactcaat tgattactca ctctactctt gaaggaccaa tcgaaaaact
    16501 tcttggttgg acaattggta acactacaac tggtgatatc aaagcaatcc ttggtgcagt
    16561 agtcttcctt gtattatata tcggtatctt tgcttggtac agaaaacaaa tgatcaaacg
    16621 taacgaagag tacgcagcaa aagcaaaata atgcgctcct ataaaatata aactcaatga
    16681 aaatcaaaga gcaaactagg aagctagccg caggttgctc aaaacacagt tttgaggttg
    16741 tagataagac tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg
    16801 aagtcagtaa tcatatctac ggcaaggcga cactgacgaa gtttgaattg gattttcgca
    16861 gagtgtaaat tgttgaaact aggttgtcat accatgtata ctacttgggt tttggcagta
    16921 gctaactgca ctaaatataa tataaggagg gttttatggg gaaaggtcat tggaatcgga
    16981 aaagagttta tagcattcgt aagtttgctg tgggagcttg ctcagtaatg attgggactt
    17041 gtgcagttct attaggagga aatatagctg gagaatctgt agtttatgcg gatgaaacac
    17101 ttattactca tactgctgag aaacctaaag aggaaaaaat gatagtagaa gaaaaggctg
    17161 ataaagcttt ggaaactaaa aatgtagttg aaaggacaga acaaagtgaa cctagttcaa
    17221 ctgaggctat tgcatctgag aagaaagaag atgaagccgt aactccaaaa gaggaaaaag
    17281 tgtctgctaa accggaagaa aaagctccaa ggatagaatc acaagcttca aatcaagaaa
    17341 aaccgctcaa ggaagatgct aaagctgtaa caaatgaaga agtgaatcaa atgattgaag
    17401 acaggaaagt ggattttaat caaaattggt attttaaact caatgcaaat tctaaggaag
    17461 ccattaaacc tgatgtagac gtatctacgt ggaaaaaatt agatttaccg tatgactgga
    17521 gtatctttaa cgatttcgat catgaatctc ctgcacaaaa tgaaggtgga cagctcaacg
    17581 gtggggaagc ttggtatcgc aagactttca aactagatga aaaagacctc aagaaaaatg
    17641 ttcgccttac ttttgatggc gtctacatgg attctcaagt ttatgtcaat ggtcagttag
    17701 tggggcatta tccaaatggt tataaccagt tctcatacga tatcaccaaa taccttcaca
    17761 aagatggtcg tgagaatgtg attgctgtcc atgcagtcaa caaacagcca agtagccgtt
    17821 ggtattcagg aagtggtatc tatcgtgatg tgactttaca agtgacagat aaggtgcatg
    17881 ttgagaaaaa tgggacaact attttaacac caaaacttga agaacaacaa catggcaagg
    17941 ttgaaactca tgtgaccagc aaaatcgtca atacggacga caaagaccat gaacttgtag
    18001 ccgaatatca aatcgttgaa cgaggtggtc atgctgtaac aggcttagtt cgtacagaga
    18061 gtcgtacctt aaaagcacat gaatcaacaa gcctagatgc gattttagaa gttgaaagac
    18121 caaaactctg gaccgtttta aatgacaaac ctgccttgta cgaattgatt acgcgtgttt
    18181 accgtgacag tcaattggtt gatgctaaga aggatttgtt tggttaccgt tactatcact
    18241 ggactccaaa tgaaggtttc tctttgaatg gtgaacgtat taaattccat ggagtatcct
    18301 tgcaccacga ccatggggcg cttggagcag aagaaaacta taaagcagaa tatcgccgtc
    18361 tcaaacaaat gaaggagatg ggagttaact ccatccgtac aacccacaac cctgctagtg
    18421 agcaaacctt gcaaatcgca gcagaactag gtttactcgt tcaggaagag gcttttgata
    18481 cttggtatgg tggcaagaaa ccttatgact atggacgttt ctttgaaaaa gatgccactc
    18541 acccagaagc tcgaaaaggt gaaaaatggt ctgattttga cctacgtacc atggtcgaaa
    18601 gaggcaaaaa caaccctgct atcttcatgt ggtcaattgg taatgaaata ggtgaagcta
    18661 atggtgatgc ccactcttta gtaactgtta aacgtttggt caaggttatc aaggatgttg
    18721 ataagactcg ctatgttacc atgggagcag ataaattccg tttcggtaat ggtagcggag
    18781 ggcatgagaa aattgctgat gaactcgatg ctgttggatt taactattct gaagataatt
    18841 acaaagccct tagagctaag catccaaaat ggttgattta tggatcagaa acatcttcag
    18901 ctacccgtac acgtggaagt tactatcgcc ctgaacgtga attgaaacat agcaatggac
    18961 ctgagcgtaa ttatgaacag tctgactatg ggaatgatcg tgtgggttgg gggaaaacag
    19021 caaccgcttc atggactttt gaccgtgaca acgctggcta tgctggacag tttatctgga
    19081 caggtacgga ctatattggt gaacctacac catggcacaa ccaaaatcaa actcctgtta
    19141 agagctctta ctttggtatc gtagatacag ccggcattcc aaaacatgac ttctatctct
    19201 accaaagcca atgggtttct gttaagaaga aaccgatggt acaccttctt cctcactgga
    19261 actgggaaaa caaagaatta gcatccaaag tagctgactc agaaggtaag attccagttc
    19321 gtgcttattc gaatgcttct agtgtagaat tgttcttgaa tggaaaatct cttggtctta
    19381 agactttcaa taaaaaacaa accagcgatg ggcggactta ccaagaaggt gcaaatgcta
    19441 atgaacttta tcttgaatgg aaagttgcct atcaaccagg taccttggaa gcaattgctc
    19501 gtgatgaatc tggcaaggaa attgctcgag ataagattac gactgctggt aagccagcgg
    19561 cagttcgtct tattaaggaa gaccatgcga ttgcagcaga tggaaaagac ttgacttaca
    19621 tctactatga aattgttgac agccagggga atgtggttcc aactgctaat aatctggttc
    19681 gcttccaatt gcatggccaa ggtcaactgg tcggtgtaga taacggagaa caagccagcc
    19741 gtgaacgcta taaggcgcaa gcagatggtt cttggattcg taaagcattt aatggtaaag
    19801 gtgttgccat tgtcaaatca actgaacaag cagggaaatt cacactgaca gcccactctg
    19861 atctcttgaa atcgaaccaa gtcactgtct ttactggtaa gaaagaagga caagagaaga
    19921 ctgttttggg gacagaagtg ccaaaagtac agaccattat tggagaggca cctgaaatgc
    19981 ctaccactgt tccgtttgta tacagtgatg gtagccgtgc agaacgtcct gtaacctggt
    20041 cttcagtaga tgtgagcaag cctggtattg taacggtgaa aggtatggct gacggacgag
    20101 aagtagaagc tcgtgtagaa gtgattgctc ttaaatcaga gctatcagtt gtgaaacgta
    20161 ttgctccaaa tactgacttg aattctgtag acaaatctgt ttcctatgtt ttgactgatg
    20221 gaagtgtaca agagtatgaa gtagatagct gggagattgc cgaagaagat aaagctaagt
    20281 tagcaattcc aggttctcgt attcaagcga ccggttattt agaaggtcaa ccaattcatg
    20341 caacccttgt ggtagaagaa ggcaatcctg cagcacctgt agtgccaact gttactgttg
    20401 gaggtgaagc agtaacaggt cttactagtc gacaaccaat gcaatatcgt actctatctt
    20461 atgatgccca attgccagaa gtcacagcaa gtgctgaaaa tgctgatgtg acagttcttc
    20521 aagcaagcgc agcaaacggc atgcgtgcga gcatctttat tcagcctaaa gatggtggcc
    20581 ctcttcaaac ctatgcaatt caattccttg aagaagcgcc aaaaattgct cacttgagtt
    20641 tgcaagtgga aaaagctgac agtctcaaag aagaccaaac tgtcaaattg tcggttcgag
    20701 ctcactatca agatggaacg caagctgtat taccagctga taaagtaacc ttctctacaa
    20761 gtggtgaagg ggaagtcgca attcgtaaag gaatgcttga gttgcataag tcaggagcag
    20821 tcactctgaa cgctgaatat gagggagcta aaggccaagt tgaactcact atccaagcca
    20881 atactgagaa gaagattgcg caatctatcc gtcctgtaaa tgtagtcaca gatttgcatc
    20941 aaaaacctac tcttccaaca acagtaacgg ttgagtatga caaaggtttc cctaaagctc
    21001 acaaagtcac ttggcaagct attccgaaag aaaaactaga ctcctatcaa acatttgaag
    21061 tactaggtaa agttgaagga attgaccttg aagcgcgtgc aaaagtctct gtagaaggta
    21121 tcgtttcagt tgaagaagtc agtgtgacaa ctccaatcgc agaagcacca caattaccag
    21181 aaagcgttcg gacatatgat tcaaatggtc acgtttcatc agctaaggtt gcatgggatg
    21241 cgattcgtat agagcaatac gctaaggaag gtgtctttac agttaatggt cgcttagaag
    21301 gtactcaatt aacaactaaa cttcatgttc gcgtatctgc tcaaactgag caaggtgcaa
    21361 acatttctga ccaatggacc ggttcagaat tgccacttgc ctttgcttca gattcaaatc
    21421 caagcgaccc agtttcaaat gttaatgaca agctcatttc ctacaataac caaccagcca
    21481 atcgttggac aaactggaat cgtagtaatc cagaagcttc agtcggtgtt ctgtttggag
    21541 attcaggtat cttgagcaaa cgctccgttg ataatctaag tgtcggattc cacgaagacc
    21601 atggagttgg tgcaccgaag tcttatgtga ttgagtatta tgttggtaag actgtcccaa
    21661 cagctcctaa aaaccctagt tttgttggta atgaggacca tgtctttaat gattctgcca
    21721 actggaaacc agttactaat ctaaaagccc ctgctcaact caaggctgga gaaatgaacc
    21781 actttagctt tgataaagtt gaaacctatg ctgttcgtat tcgcatggtt aaagcagata
    21841 acaagcgtgg aacgtctatc acagaggtac aaatctttgc gaaacaagtt gcggcagcca
    21901 aacaaggaca aacaagaatc caagttgacg gcaaagactt agcaaacttc aaccctgatt
    21961 tgacagacta ctaccttgag tctgtagatg gaaaagttac ggcagtcaca gcaagtgtta
    22021 gcaacaatgg cctcgctacc gtcgttccaa gcgttcgtga aggtgagccg gttcgtgtca
    22081 tcgcgaaagc tgaaaatggc gacatcttag gagaataccg tctgtacttc actaaggata
    22141 agaacttact ttctcataaa ccagttgctg cggttaaaca agctcgcttg ctacaagtag
    22201 gtcaagcact tgaattgccg actaaggttc cagtttactt cacaggtaaa gacggctacg
    22261 aaacaaaaga tttgtcagtt gaatgggaag aagttccagc agaaaatctg acaaaagcag
    22321 gtcaatttac cgttcgaggc catgtccttg gtagtgatct tgttgctgag gtcactgtac
    22381 gagtgacaga caaactaggc gaagctcttt cagataaccc taactatgat gaaaacagta
    22441 accaggcctt tgcttcagca accaatgata ttgacaaaaa ctctcatgac cgcgttgact
    22501 atctcaatga cggagattat tcagaaaatc gtcgttggac aaactggtct gcaagaccgt
    22561 ctaccaatcc agaagtatca gcaggcgtaa tcttccgtga aaatggtaag attgtcgaac
    22621 ggactgttgc gcaagccaaa cttcatttct ttgcagatag tggtacagat gcaccatcta
    22681 aacttgtttt ggaacgttat gtcggcccag gctttgaagt acctacctac tattcaaact
    22741 accaagccta cgaatctgga catccattta acaatccaga aaattgggaa gctgtgcctt
    22801 atcgtgcgga taaagacatc gcagctggtg atgaaatcaa cgtaacattt aaagctgtca
    22861 aagccaaagc catgagatgg cgtatggagc gtaaagctga caagagcggt gttgcgatga
    22921 ttgagatgac cttccttgca ccaagtgaat tgcctcaaga aagcactcaa tcaaagattc
    22981 ttgtagatgg aaaagaactt gctgatttcg ctgaaaatcg tcaagactat caaattacct
    23041 ataaaggtca acggccaaaa gtctcagttg aagaaaacaa tcaagtagct tcaactgtgg
    23101 tagatagcgg agacgatagc cttccagtac ttgttcgcct cgtttcagaa agtggaaaac
    23161 aagtcaagga ataccgtatc cacttgacta aggaaaaacc agtttctgag aagactgttg
    23221 ctactgtaca agaagatctt ccaaaactcg aatttgttga aaaagatttg gcctacaaga
    23281 cagttgagaa aaaagattca acactgtatc taggtgaaac tcgtgtagaa caagaaggaa
    23341 aagttggaaa agaacgtatc tttacagcga ttaatcctga tggaagtaag gaagaaaaac
    23401 tccgtgaagt ggtagaagct ccgacagacc gcatcgtctt ggttggaacc aaaccagtag
    23461 ctcaagaagc taaaaaacca caagtgtcag aaaaagcaga tacaaaacca attgattcaa
    23521 gtgaagctag tcaaactaat aaagcccagt taccaaatac aggtagtgcg gcaagtcaag
    23581 cagcagtagc agcaggttta gctcttctag gtttgagtgc aggattagta gttactaaag
    23641 gtaaaaaaga agactagaaa ttttgatacc ttctttatca ttaaagacca aagaaaaact
    23701 cttgtcctaa atagaagata aagagtctgg gacaaaatag ttctcaacca caaaaagcta
    23761 gagatttcca attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt
    23821 agattgaaac tagaatagta cacttctact tctaaaacat tgttagaaat cgatttgact
    23881 gtccttatcg atttgtcctg ttcttatttc atttcactat attttaggag gttattggcc
    23941 ataagtacca atcccatgtc aattctcact tgacgcttcc ctctcagatt acatctcttg
    24001 taacccaaac aagcctttat ctgcccaaag acaggttcca catcaatctt gcgttgagcg
    24061 aaaatctgtc taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga
    24121 tagcgttcgt tcatatacag tcccttttga gggactgatt caggttcatc agcgtagtaa
    24181 accttgattt cctgttgaaa gtctgtctgt gttttctggt acttgatatg gtgaaaacga
    24241 tagcaccagc catcaggatg tgtgtagcta tcctccttgt cattatagtg ccaattcgct
    24301 aagtttttag ctgactgttt ataccctctc ttttgtttct tatcaaacat ggcatattta
    24361 atcagaaggg gagtgatttc ttcttgaaaa tagacctgta tctgttcttg aagtttggcg
    24421 gagaatttct ctgtcgtttt cttccacaca aaactatact tgttggcatt ggcttcaatc
    24481 ttagttccgt caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc
    24541 gaccagagaa aatcccttgt gaataggcaa atagaagagt agatacaagc attttagggt
    24601 gataagacgg gcgatcaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat
    24661 tcaccacttt ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta
    24721 gtggtaaagt tgtttgattt gtgttatagt gaatatgcat gggatagcct ttttaaatgg
    24781 tttattaagc aattctattt taccaagact atcgtaacca tgcaaaaaaa gcaacggcaa
    24841 atccgttgct ttttttggag ataagagact attgtcccag cctcttttct ttgtataaaa
    24901 ttgtatagtg ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct
    24961 tagggattcg actatttcat taagaacaat tcaacaatca tttctatata catgataagg
    25021 gttaataaga atcctgcacg ccagaagaat ttgataaatt taggatagta aaagctgcgt
    25081 tttttcaaaa ggaagaaaaa gacgagaata atggctagga acgagagggc tagccccagt
    25141 ctagggagga aattatgggt aaaggtttta gctgtaatca agtaatactc aaataccaag
    25201 actggaaaag ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt
    25261 aggataatgc agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa
    25321 atcataccca tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat
    25381 aggaaagtca ggcttttgag attgtgggtg atttttgtta taatgaaagt tataaaatct
    25441 tataatacct tgaatgatta ttatcgaaaa ctctttggag aaaagacttt taaagtccct
    25501 attgatgcgg gatttgactg tcccaatcgt gatggaactg tggctcatgg aggctgtact
    25561 ttttgtacgg tttccggttc tggagatgcc attgtggcac cagatgcgcc tatccgtgag
    25621 caattttata aggaaattga ctttattcat cgtaagtggc cagatgttta gaagtatctg
    25681 gtttattttc aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat
    25741 gaacaggcta tcaatgagcc aggtgtggta ggaatcaata ttggaacgcg cccagactgt
    25801 ttaccagacg aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgacggtt
    25861 gaattgggct tgcaaactac ttatgaagca acctctgacc tgattaaccg tgcgcattct
    25921 tatgaattgt aggtggaaac ggtcaagcgt ttgagaaagt atccaaaaat tgagattgtt
    25981 tcccatttga tcaatggttt gcctggtgaa acccatgaga tgatggttga aaatgtccgc
    26041 cgttgtgtca cggataacga tattcaaggg attaaactgc atttgctcca tcttatgaca
    26101 aatactcgta tgcaacgaga ttaccatgag gggcgtttac aattgatgag tcaggacgaa
    26161 tatgtcaggg tcatctgtga ccaactggaa attattccca aacatatcgt catccatcga
    26221 attacaggag atgcgcctag ggatatgctg cttgggccta tgtggagcct caataaatgg
    26281 gaagctctca acagcattga gatggagatg cgacgtcgtg gaagtgttca aggatgcaag
    26341 gctgtaaaac aggagtttga aaatgaaaag accacttgag atggcacatg attttttggc
    26401 tgaggtcgtg acaaaagagg atgtcgtagt ggatgcgact atgggaaatg gtcatgacac
    26461 gcttttttta gccaagctag ccaagcaagt ctatgccttt gatattcaga agcaagcctt
    26521 ggaaaagacc caagagcgtt tgcatcaggc tgacttgaca aatgcccagt taatcttgca
    26581 aggccatgag acactggacc agtttgtgat aaaagctaag gcagggattt ttaatctggg
    26641 ctatttgccg tcagctgata agtctgtcat cacccgaccg cagacaacga ttgaggcatt
    26701 agaaaagcta tgtggcttac ttgtcaaagg tggacgaatt gctattatga tttactatgg
    26761 tcatgaagga ggcgatctcg agagagatgc tgtcttggat tttgtgatcc agttgaacca
    26821 acaagagtac acagctgcca tttaccgaac tttaaaccaa gtcaacaacc cgccgttttt
    26881 agtgatgatt gaaaaattag agagatacag acatggataa acaataccta cacgaaaagc
    26941 tggatgccat gcgccagaat tttgttgaat caacccacca cgaacgatcg gtgggcgtgc
    27001 tagaccaagc gcatatgagc aaaaaaatgc ttaaaatcaa gaaaaaatta gttgctcttg
    27061 aaatggaacg gtgccagaga aaaattgagc acaaagactg ttccaagatt gaccaaaaaa
    27121 tcaaagagca gaaggagata tttgaatcct gttgtaaaaa agattaagga ggacgtgcgt
    27181 ggaattactg atttacctca tcctattttt actggttttg attgtctcaa gtacaaccaa
    27241 taagctcctg ccctttttgc ctctcccttt ggtgcagatt cttttgggaa ttgtgattgg
    27301 tctcttttta cccaatactg actttcacct taatacggag ttgtttttgg cactggttat
    27361 cggacccttg cttttccgag aggctgaaga agcagatgtt acggctattt taaaacactg
    27421 gcgaatcatt gtttatctca tatttccagt gatttttatc tcgaccttga gtttgggtgg
    27481 cttggcccat cttctttggt tcagccttcc cttggcagct tgcttggctg ttggggcagc
    27541 ccttggtcct acggacttgg tggcctttgc ctctctttcg gagcgtttta gctttcctaa
    27601 gcgcgtgtcc aatattctta agggcgaagg actcttgaat gatgcttctg gtttggtggc
    27661 ttttcaggta gctttgacag cttggacaac tggagctttt tctctggggc aagctagcag
    27721 ttcgctcatc ttttcaatcc taggcggttt tttaattgga tttttaacgg ccatgaccaa
    27781 ccgcttcctc cataccttct tgctaagtgt gcgcgcaacg gatattgcca gtgaactttt
    27841 attagaattg agtttgcctc tagtgacctt ctttctggca gaagaagttc atgtttcagg
    27901 tattattgcc gtcgtagttg ctggaatttt aaaggcaagt cgcttcaaga aaatcacgct
    27961 cctcgaagcc caagtggata cggtgaccga gacggtctgg catacagtga cctttatgct
    28021 caacggttct gtctttgtga ttttagggat ggagttggaa atgatagcag aacctatctt
    28081 gaccaatcca atctataatc ctctactttt attgctatct ctcatcgccc ttacctttgt
    28141 cctctttgtc attcgtttta ttatgatcta tggctattat gcctatagaa accgacgcct
    28201 caagaaaaag ctaaataagt atatgaagga catgtttctc ttgacctttt caggtgttaa
    28261 gggaacggtg tcgattgcta cgattctctt gataccaagt aatctagaac aggagtatcc
    28321 tcttttgctt ttccttgttg caggtgtgac gcttgtcagc tttttaacag gtctcttggt
    28381 cttgcctcat ctttctgatg aagaggaaga aagcaaggat tatctcatgc atatcgccat
    28441 tttgaatgaa gtaacgctag agttggaaaa agagttggaa gacaccagaa ataaacttcc
    28501 cctctatgcg gctattgaca attatcatgg acgtattgaa aatctcattt taagccaaga
    28561 aaaccaggat gatcaagaag attgggctgc tttgaaactc ttgattctta gtattgaaag
    28621 tgatggtttg gaacaggcct atgaagaggg gaacattagc aatcgtgctt accgagttta
    28681 ccaacgttat ctgaaaaata tagaacaagg aatcaatcgt aaacttgcct caagactgac
    28741 ctattatttc cttgtttctt tgcggatttt acgttttctc cttcatgaag tttttactct
    28801 cggaaagacc ttccgtagct ggaagaacaa ggagcaaagc cgtctccgtg ctcttgatta
    28861 tgaccaaatt gcagagctct atcttgccaa tacggagatg attattgaaa gtttggaaaa
    28921 cctgaaggga gtctacagac gctctttgat tagttttatg caggagtctc gtcttcgaga
    28981 aacaactatt atcagcagtg gtgcctttgt cgaacgggtt atcaatcgtg tcaaacccaa
    29041 caatatcaat gaaatgctga gaggctatta tctggagcgc aagttgattt tcgaatacga
    29101 agaaaaacga ttaattacaa ctaagtatgc caagaaatta cgacaaaatg taaataactt
    29161 agagaactat tccttgaagg aagctgccaa taccctgccg tatgatatgg tggaattggt
    29221 aagaagaaat tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgc
    29281 cgtatgtatg tgactgactt cgtcagtctt atctacaacc tcaaaacagt gttttgagca
    29341 gcttgcggct agctttctag tttgctcttt gattttcatt gagtataaga ttataaatga
    29401 aggagtatga catgaaaaag tggtggaaag agctgataga caagccttta ttaaaagctt
    29461 ttttgcatta ttatcaagca tcagatagtg agttgaccag tgtcgcagta gcctactatt
    29521 ggttgatttc gattttcccc ctgcttttgg tggtggtcaa tatcctcccc tattttcaga
    29581 ttcctgtggg agaatttctg ggctttatga aagacgtcct gcccccaagc ctctatgaag
    29641 gtgtggaaaa aatagccaga gaagtcttga cgcaaccttc aacaggttta ttgagttttt
    29701 ctgttttatc ggcgttatgg agtttttcaa aatctatgaa tttcttgcaa aaagctttta
    29761 acaaggctta tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg ctaagtctcc
    29821 tagtcagttt tggcttgcag ctcctctttg cgtttgcctt gtttttgatt ttatttggtc
    29881 agatgatttt ggctttactt gctcattatt ggacaaaaga tggcattatc tatcaggctt
    29941 tacaagggtt ggcaggtcct ctgatttacg ccctcctctt tgctatcttg gtcatgcttt
    30001 attattttct tcccaatcta tctaacagga aaattagcta taccctacca ggcagtgcct
    30061 ttgttctctt agtgatttta ggtttactaa ctctgttctc tagttatctc aattactatg
    30121 tccatcattt ggtggatgtc cgaattttag gttccgttct tcttgttgtt atgatgtttt
    30181 ggtttatctt gattgctaaa attgttatcc taggcgcggt tatcaatgcc agtatgcaga
    30241 gtttgaaaga tccggtcttt aaaaaagact aactatttat ccattacaaa agcgcatagt
    30301 atcaagactt tatagaacct tgatactatg cgcttttgtg ctttgacagt tttttgtata
    30361 gtttttgtcc aagatttcta aatcatctga ttaaattcct attttcccct atcattgtcc
    30421 ctgttttttc gaaaatggaa ggagtataat aaatctatca ataaaatgat aggaggaact
    30481 ttatgggtca tattttcttt tttctaagtg tctttttggc agggattcta tccttctttt
    30541 ctccttgtat cttacctttg ttaccggtct atacaggagt gttactagat gataaggatg
    30601 gtgctcaggc ttctagcggc aaattttcaa tctcagttac tagtttatta cgaactctgg
    30661 tctttatagc aggaatttcc tttatattta ttttgttggg ctatggagct ggttttttag
    30721 gcgatttgct ttatgcttct tggttccaat atcttactgg ggcaattatt atccttcttg
    30781 gtttgcacca aatggagatt ctacacttta aggggcttta taaggaaaag aggctacaac
    30841 tgcaaggaca ggggcaaaat ggtaagggct atagtcaggc atttttattg ggcttgacct
    30901 ttagttttgc ttggacgcct tgcgtggggc cggttctggg gtctgttttg gccttggcgg
    30961 cttcaggtgg ttcaggagct tggcagggag ctggtctcat gttggtgtat acgctgggct
    31021 tggcgctacc attcttgctt ctagctctga cctctagtta tgttttgaaa catttccgaa
    31081 aacttcatcc ctatctcgga atcctcaaaa aagtgggtgg ttttctcatt attgtgatgg
    31141 gattcttggt tctgtttgga aatgcttcaa ttttaagtca attatttgaa taaaatggaa
    31201 aggaatatca atatgaaaaa atggcaaaca tgtgttcttg gagcaggttc gctcctttgt
    31261 ttgacggctt gttcaggcaa gtccgtgact agtgaacacc aaacgaaaga tgaaatgaag
    31321 acggagcaga cagctagtaa aacaagcgca gctaaaggga aagaggtggc tgattttgaa
    31381 ttgatgggag tagatggcaa gacctaccgt ttatctgatt acaagggcaa gaaagtctat
    31441 ctcaaattct gggcttcttg gtgttccatc tgtctggcta gtcttccaga tacggatgag
    31501 attgctaaag aagctggtga tgactatgtg gtcttgacag tagtgtcacc aggacataag
    31561 ggagagcaat ctgaagcgga ctttaagaat tggtataagg gattggatta taaaaatctc
    31621 ccagtcctag ttgacccatc aggcaaactt ttggaaactt atggtgtccg ttcttaccca
    31681 acccaagcct ttatagacaa agaaggcaag ctggtcaaaa cacatccagg attcatggaa
    31741 aaagatgcaa ttttgcaaac tttgaaggaa ttagcctagg aggcgtctta tgaatgataa
    31801 gttaaaaatc ttcttgttgc taggagtatt ttttctagcc ataaccggtt tctatgttct
    31861 attgatacga aatgcagggc agacagatgc ctcgcaaatt gaaaaggcgg cagttagcca
    31921 aggaggaaaa gcagtgaaaa aaacagaaat tagtaaagac gcagacttgc acgaaattta
    31981 tctagctgga ggttgtttct ggggagtgga ggaatatttc tcacgtgttc ccggggtgac
    32041 ggatgccgtt tcaggctatg caaatggtag aggagaaaca accaagtacg aattgattaa
    32101 ccaaacaggt catgcagaaa ccgtccatgt cacctatgat gccaagcaaa tttctctcaa
    32161 ggaaatcctg cttcactatt tccgcattat caatccaacc agcaaaaata aacaaggaaa
    32221 tgatgtgggg acccagtacc gtactggtgt ttattacaca gatgacaagg atttggaagt
    32281 gattaaccaa gtctttgatg aggtggctaa gaaatacgat caacctctag cagttgaaaa
    32341 ggaaaacttg aagaattttg tggtggctga ggattaccat caagactatc tcaagaaaaa
    32401 tccaaatggc tactgccata tcaatgttaa tcaggcggcc tatcctgtca ttgatgccag
    32461 caaatatcca aaaccaagtg atgaggaatt gaaaaagacc ctgtcacctg aggagtatgc
    32521 agttacccag gaaaatcaaa cagaacgagc tttctcaaac cgttactggg ataaatttga
    32581 atccggtatc tatgtggata tagcaactgg ggaacctctc ttttcatcaa aagacaaatt
    32641 tgagtctggt tgtggctggc ctagttttac ccaacccatc agtccagatg ttgtcaccta
    32701 caaggaagat aagtcctaca atatgacgcg tatggaagtg cggagccgag taggagattc
    32761 tcaccttggg catgtcttta cggatggtcc acaggacaag ggcggcttac gttactgtat
    32821 caatagcctc tctatccgct ttattcccaa agaccaaatg gaagaaaaag gctacgctta
    32881 tttactagat tatgttgatt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    32941 cttaccgtat gtagggttac tgacttcgtc agttttatct acaacctcaa agctatactt
    33001 tgagctgact tcgtcagttt tatttacaac ctcaaagcgg tactttgaga aacctgtggc
    33061 tatcttccta gtttactttt tgatttttat tgagtataag aggtctttcc taagcagtta
    33121 gaggagaatt ttgctatact gatactagta agtggtaaag gagcagatca tgacctacac
    33181 aatcttaatc gtagaagatg aatatctggt aagacaaggt ttgactaaac tggtcaatgt
    33241 agcagcctac gatatggaaa tcatcggtca ggctgaaaat ggaaggcagg cttgggaatt
    33301 gatccaaaag caggtgccag atatcatttt aaccgatatc aacatgcctc atctaaatgg
    33361 catccagttg gccagtctgg tacgagaaac ctatcctcag gttcatttgg tctttttaac
    33421 aggttacgat gattttgatt atgccttgtc tgctgtcaaa ctaggtgtgg acgactacct
    33481 gctcaaaccc ttttctcgtc aggatattga ggaaatgttg gggaaaatca aacaaaaact
    33541 agacaaggaa gagaaagaag agcagttaca agatttattg actaataggt ttgaaggaaa
    33601 catggcccag aaaatccagt ctcatctggc tgatagccaa tttagtttaa agtctttagc
    33661 cagtgactta ggttttagtc cgacctatct gagttccttg attaagaaag agttgggctt
    33721 gccttttcag gattatctgg tgagagaacg tgttaaacaa gccaagctct tgcttttaac
    33781 tacagatctg aagatttatg agatcgcaga gaaggttggt tttgaagata tgaactattt
    33841 tacccaacgt tttaagcaga ttgcaggtgt gacacctcgt cagtttaaga agggagaaga
    33901 ccgatgaagc gttcttctct tttagttaga atggttattt ccatctttct ggtctttctc
    33961 attctcctag ctctggttgg aactttctac tatcaatcaa gttcttcagc cattgaggcc
    34021 accattgagg gcaacagcca aacgaccatc agccagacta gccactttat tcagtcttat
    34081 atcaaaaaac tagaaaccac ctcgactggt ttgacccagc agacggatgt tctggcctat
    34141 gctgagaatc ccagtcaaga caaggtcgag ggaatccgag atttgttttt gaccatcttg
    34201 aagtcagata aggacttgaa aactgttgtg ctggtgacca aatctggtca ggtcatttct
    34261 acagatgaca gtgtgcagat gaaaacttcc tctgatatga tggctgagga ttggtaccaa
    34321 aaggctattc atcagggagc tatgcctgtt ttgactccag ctcgtaaatc agatagtcag
    34381 tgggtcattt ctgtcactca agaacttgtt gatgcaaagg gagccaatct tggtgtgctt
    34441 cgtttggata tttcttatga aactctggaa gcctatctca atcaactcca gttggggcag
    34501 cagggctttg ccttcattat caatgaaaac catgaatttg tctaccatcc tcaacacaca
    34561 gtttatagtt cgtctagcaa aatggaggct atgaaaccct acatcgagac aggtcagggt
    34621 tatactcctg gtcacaaatc ctacgtcagt caagagaaga ttgcaggaac tgattggacg
    34681 gtacttggcg tgtcatcatt ggaaaagtta gaccaggttc ggagtcagct cttgtggacc
    34741 ttgcttgggg ccagtgtcac atctcttctt gtctgtctct gcttagtgtg gttcagtctt
    34801 aaacgctgga ttgctccttt gaaggatttg agagaaacca tgttggaaat tgcttctggt
    34861 gctcaaaatc ttcgtgccaa ggaagttggt gcctatgaac tgagagaagt aactcgccaa
    34921 tttaatgcca tgttggatca gattgatcag ttgatggtag ctattcgtag ccaggaagaa
    34981 acgacccgtc agtaccaact tcaagccctt tcgagccaga ttaatccaca tttcctctat
    35041 aacactttgg acaccatcat ctggatggct gaatttcatg atagtcagcg agtggtgcag
    35101 gtgaccaagt ccttggcaac ctatttccgc ttggcgctca atcaaggtaa ggacttgatt
    35161 tgtctctctg acgaaatcaa tcatgtccgc cagtatctct ttatccagaa acaacgctat
    35221 ggagataagc tggaatacga aattaatgaa aatgttgcct ttgataattt agtcttaccc
    35281 aagctggtcc tacaacccct tgtagaaaat gctctttacc atggcattaa ggaaaaggaa
    35341 ggtcagggcc atattaaact ttctgtccag aaacaggatt cgggattggt catccgtatt
    35401 gaggatgatg gcgttggctt ccaagatgct ggtgatagta gtcaaagtca actcaaacgt
    35461 gggggagttg gtcttcaaaa tgtcgatcaa cggctcaaac ttcattttgg agccaattac
    35521 cagatgaaga ttgattctag accccaaaaa ggaacgaaag ttgaaatata tataaataga
    35581 atagaaacta gctaactccc agtcaattct gggagtttta tgcgtaattg ggcagacttt
    35641 ataggttgtc tatcttgcta aaaacaagaa aaaacggtat gatagaagag tgacaacaga
    35701 ggtaacagag atgaacgaga cacagattca aagagaaaca cgtcaggtag tggaagatgt
    35761 tttagaaaag accaatttga agcagggggt cctttttgtt ttaggcctat cttctagtga
    35821 ggtgctaggt ggtcagattg ggaaagaatc cagtcaagag attggtgagc tgatagtgga
    35881 gactatcttg ggtattctag gtagtagagg tatccattta gcagttcaag gttgtgagca
    35941 tgtcaatcgg gctctcgttg ttgaacgtca ggtggcagag cagtttgatc tggaaattgt
    36001 cagtgtccat cctactcttc atgcaggagg ttcggggcag ttggcagcct ttaagtttat
    36061 gcaggatcca gttgaggttg aatttatcaa ggctcatgct ggattggata tcggagacac
    36121 tgcaattggc atgcatgtca agcatgttca ggttccgatt cgccctattt tgagagagat
    36181 tggtcatgcc catgtaacgg cactggctag tcgtccaaaa ttaatcggag gtgcgcgtgc
    36241 gcactatccg caagatgcta ttagaaagtc ttgagaatga ataaaggaga tgaaagtgtt
    36301 taaaaaagac cgtttttcaa ttcgtaagat taagggagtt gtaggctctg tatttcttgg
    36361 aagccttttg atggctcctt ctgtagtgga cgcagctacc tatcactatg taaataaaga
    36421 gattatttca caagaagcta aagatttaat tcagacagga aagcctgaca ggaatgaagt
    36481 tgtatatggt ttggtgtatc aaaaagatca gttgcctcaa acagggacag aagcatctgt
    36541 tttgacagct tttggtttgc tgactgttgg gagcttgctt ttaatctaca agagaaagaa
    36601 aattgctagc gtctttctag ttggagctat gggattggta gttcttccta gtgcaggggc
    36661 tgtagaccca gttgcgaccc tagcgctggc tagtcgagag ggtgttgttg aaatggatgg
    36721 ctatcgctat gttggttatc tatcaggtga catcctcaaa acgcttggct tggacactgt
    36781 tttagaagaa acctcagcaa aacctggaga ggtgactgta gtcgaagttg agactcctca
    36841 atcaacaaca aatcaggagc aagctaggac agaaaaccaa gtagtagaga cagaggaagc
    36901 tccaaaagaa gaagcaccta gaacagaaga aagtccaaag gaagaaccaa aatcggaggt
    36961 aaaacctact gacgacaccc ttcctaaagt agaagagggg aaagaagatt cagcagaacc
    37021 agctccagtt gaagaagtag gtggagaagt tgagtcaaaa ccagaggaaa aagtagcagt
    37081 taagccagaa agtcaaccat cagacaaacc agctgaggaa tcaaaagttg aaccaccagt
    37141 agaacaagca aaagtcccag aacaacccgt gcaacctaca caagctgagc aaccaagtac
    37201 accaaaagaa tcatcacaac aagaaaatcc taaagaagat aggggagcgg aagagacacc
    37261 gaaacaagaa gatgaacagc cagcagaagc cccagaaatc aaggttgaag aaccagtaga
    37321 atcaaaagag gagactaaaa cagctaaggg gacgcaagaa gaaggcaaag aaggtcaagc
    37381 accagtacaa gaagtaaacc cagaatataa agtaacaaca ggtacagttg agaagtctac
    37441 cgaaagtgaa ttagatttca caacagaagt agttccggat gacacaaaat atgtagatga
    37501 agaagttgtt gaacgtcaag ggtctaaagg agttcaagtt acgaaaacaa cttacgaaac
    37561 agtggaagtt gtagagacag acaaagtcct ttctacaact acagaagtga aaactcctgt
    37621 tgttcctaaa gtcgttaaaa aaggaacaaa accagttgaa ggaacagcag ttgaaacaag
    37681 agaagaagtc attccttttg ctacaaaaga acaagaagat ggcactttaa aacgcggaac
    37741 aagacaagta gctcaagaag gtgtaaacgg taagaaacaa attactgaaa cctataaaac
    37801 aattcgcgca gaaaaaacaa acgaagctcc aacagtagaa gaaacagttc tccaagcacc
    37861 tcaagatgag attatcaaaa aaggcacaaa aggtttagaa aaaccaacct tagaatgggt
    37921 taaaacagat aaagatgtct tgaagaagag tgcgacagcc agttatacat tgaataaacc
    37981 agatggggta gaaatcaaat ccatcaaagt agctctaaaa gataatactg gaacagttat
    38041 taaagaagta acggtagaag aaaataactt aaacgttact ttagacaact tgaaatatta
    38101 tcaaggttat acactatcca caaccatggt ctataatcga ggcgaaggcg aagaaactga
    38161 aacgttagaa gataaagaag ttcagttaga ccttaaaaaa gttgaaatca aaaacatcaa
    38221 agaaactagc ttgatgagtg tggatgatgc tggtgttgaa acagataaga gcttacttac
    38281 tgaaaaaccg acagacgttg ctccacttta cctacgagtg acaactcatg ataacaaagt
    38341 aacaagactt gctgttgata agattgaaga agtagaagaa gacggaaaaa ctttatacaa
    38401 agttactgcc aaagcaccag acttagttca acgtaatgct gataacacac ttagcgaaga
    38461 gtatgttcat tactttgaaa aacaacttcc aaaaattgga aatgtgtatt acaacttcaa
    38521 cgagttgatt acagatatgc aaaaaacacc gaatggcgag tttaaacttg gtgcagactt
    38581 gaatgctgtc aatgttccaa caccaaataa atcttatgtg actgctaaat ttactggtaa
    38641 gttgtatagt gaaggtgata aacactatac tattcacaac ttagcacgtc cattgtttgc
    38701 gcaagctgaa aacgctcata ttcatgacat caacctcggt aatgtaaata ttaatatgcc
    38761 gtgggcgaac aaaacagcac ctcttggtga aatgttcaag aagtcaacta ttgaaaacgt
    38821 caaagtaaca ggtaatgttg tcggtaacaa tgacgttaca ggtatggtca acaaactgga
    38881 cgaatctgat atgcgtaacg tagctttcat tggaaatatt acaagtgtag gtagcgctgg
    38941 atggtggtct ggtggacttg tcagtgaaag ctggagaagt aacacagata attcttatat
    39001 tgataccacc atcaaaggta ataaagctaa ggtaggtgga ttaattgcta agttaaatca
    39061 cggtgctgac ccacgagatg ttggtgcgag aggtcgtttg aagaactctg tcgcaaaagg
    39121 tactattgat gtgagagacc cacaagaaac tggtggattg ctgcattcta actggtcatg
    39181 gggtcttgca gaaaacaata ttaccatgat gaaagtgaag aatggaggag aaattttata
    39241 tggttctcgt gatgcagaag atgatgatta cttcggagcg aattgggtgc gtaataataa
    39301 tgcgttcgtc aatggaatca gtgaagggaa acaatcctat tctcgttcta gtcgctggaa
    39361 agggatttct gaagatgaag ctaaaactcg tattgccaaa atgggaatta cagctcatga
    39421 atacgaaatt acacaacatt tgactgataa attgaaccgt gcagcattta aagaagacac
    39481 atataaaaca actcaagatt acaaaactga aagagaatta gcgtatcgta atattgaaaa
    39541 gcttcaacca ttctacaata aagaatggat tatcaaccaa ggtaacaaat taacagacgg
    39601 ttcaaactta atgattaaag aggtattgtc cgttataggt atgaagaacg gtcagttcgt
    39661 gaccgatttg tctgatattg ataagattat gattcactac gctgatggta cgaaagaaga
    39721 aaaaactgtc acacgtaaag ctgactcaaa agtgcaacaa attcgtgaat atagtgttga
    39781 aggtcttggt gatgttgtgt acacaccaaa tatggtcgaa aaagatagag cgcaattaat
    39841 tactgatatt aaggcgaaat tggatagcgt ccaattgatt tcaccagaag tgcgcaattt
    39901 gatggataaa cgtggcaaag cgcatgaaaa cacggatgaa cgtcgtaatg gctacattcg
    39961 aaacctattc cttgaagaaa gtttggatga agtgaaagga aaccttgata agcttgtgaa
    40021 agctcttgtt gaaaatgaag accatcaatt gaatggtgat gaagcggcaa tgaaagctct
    40081 tgtcaagaaa gttgaagaca acaaagcgaa aatcatgtta ggtcttgctt atctcaatcg
    40141 ctactatgga ttcaaatatg acgaaaagtc tatgaaggat attatgatgt tcaaaccgga
    40201 cttctatggt aagaatgtta gtgtcttgga cttcttgatt agagttggtt caagagaaca
    40261 caatattaaa ggtaacagaa cgttagaagc ttatcgtgaa gtaattggtg gaacgattgg
    40321 tatcggtgag ttaaacggtt tcttaaacta taatatgcgt ttattcacag aagaaactga
    40381 catcaatact tggtacaaaa aagctgtatc gcataccaac tatattgttg aaaaacaatc
    40441 ctcaaaccct gcgtttgcca acaagaaata tcgcctttat gaaaacttga acaatggtga
    40501 acatggtaaa tatattttac cacttctcac tacgaagaaa gctcatatgt tcttgatttc
    40561 aacctataat acattagcgt ttagtgcgtt tgagaaatat ggtaagaata cagaagctga
    40621 acgtgaagcg tttaagaaag aaatcgattt acgtgctcaa gagcaaatta attacttaga
    40681 cttctggtca agacttgcag ctgataatgt acgtgacaga ttgttaaaat ctgaaaatat
    40741 ggttccgtct gcaatttggg ataaccaaga agttccaggt catggatggg ctgatagaat
    40801 gggtcataat aagaatggtg actatgctcc ggttcgtgag ttctatggac ctacaggtaa
    40861 atggcatgga tacaatggta cgggtgcata tgcttatatc tttactaatc cacaaaactc
    40921 agaagctgta tattatatca tatctagtat gatcagtgat ttcggtactt ctgcattcac
    40981 tcatgaaact acacacatta atgaccgtat ggcgtatcta ggtggatggc gtcaccgtga
    41041 aggtactgac gtagaagcat ttgcccaagg tatgttacaa tcaccggctg tctcaagtcc
    41101 gaacggcgac tacggtgctt taggtcttaa catggcttac aaacgtgaaa acgatggtaa
    41161 ccaatggtac aactatgatt caaataaatt agatagtcgt gcgaagattg accattacat
    41221 gaagaattac aacgaagcgc tcatgatgct tgaccactta gaagctgatg ccgttatcgc
    41281 gaagaataac ggagataaca acaagtggtt caagaagatg gataagaaat ggcgtgagaa
    41341 cgctaatcga aatggattag tgggacaacc acaccaatgg gatttacttc gtgacctaaa
    41401 tgaagaagag aataagaaga aattgacaag cattgatgac cttgtagatg gaaattatgt
    41461 aacgaagcat aatatgccgg gtaataaaca ctatcgtgct gaagggtttg atacagcgta
    41521 tcaaactgta aacatgatgg ctggtattta cggtggtaat actagtaaga gtgctgttgg
    41581 ttctatctcg ttcaaacata acaccttccg tatgtggggt tacttcggtt atcttgatgg
    41641 atttgtaggt tatgcatcta acaaatataa agacgcggct aacaaagaaa ataaaggctt
    41701 acttggcgac gactttatta tcaagaaagt gtctgacggt aaatttgaca gcctagaagc
    41761 ttggaagaaa gaatggttta aagaagttaa agctaaaggt gaaaaaggat ttgttgctat
    41821 tgaaattgat ggtaaaacaa tcactaacta tgctgaactt cgtgaattgt tcgacaaagc
    41881 tgttgaagct gacctgaaag ctggtaactc taaccaaaca gttgccctta aagaaaaggt
    41941 ttataaacaa ctcttgcaga aatcagatgg atttgtagga aatcttttta aagcatagat
    42001 tgtattaaat agatgagtaa aaaaggtaga ttaagttttt cttgatctgc ctttttatgc
    42061 tatacttaag gtatgcatag aaaaacagtg attgatttta ggactttggg ggagagatat
    42121 acctttaccc agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg
    42181 ctggcacaag tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac
    42241 gaggctgcac ctgcttttga ggagaaatta gcagttcaca aggttcctct actgggcgag
    42301 tacttgcttt actttactgt tcacgatagg gtagagacct cccctattcc tctgacttat
    42361 gaggatattg atttgccctc aaattggcag gaagtgacgt ctgcagcaga ctatgaaaag
    42421 gccattgccc agatacacca tcacttgagg cagggagaca cctatcaggt caactacacc
    42481 gtccaactca agcaaaagtt aagtgccaat ccttttgcca tctacaatcg tatggtggta
    42541 gagcaggagg cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg
    42601 agcccagagc tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg
    42661 acgactcagc gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag
    42721 gatcccaaaa atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac
    42781 cgtatttctg aagttgggag cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca
    42841 actgtttggc agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt
    42901 gaaatcttcc gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca
    42961 atggagatta tcaaggactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt
    43021 ctcttgcttc caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac
    43081 aaaggtcaag ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct
    43141 gaataccgag aggttcatca aaaggcagca gttctttatc gtaaacaagc tcgcttccaa
    43201 ctgattacga caggggaaat cagccagaag aacctgctgt ttgaagatca acatctggaa
    43261 agactgagaa aagctagtcg ttattttgct tttccttttg atgcagaaga cttgggacac
    43321 aagattgagg aagagtgtca ggattgtgaa gctaatcgag attaccgctt gcgaatcagc
    43381 cttagcaaat ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc
    43441 ttttgtcagg cccaagtctg tcttcaggaa gctgctttga atcaatcctt tacctacttt
    43501 aaaaccactc accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag
    43561 tcaggagaac tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc
    43621 tacacaccgc ctatccgact tggaatcttg ccaggaattt accgtcagca tttgctagaa
    43681 acaggacagg tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt
    43741 tacggctgta atgcagtgag aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa
    43801 ttgatatttt tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta
    43861 ctgacagatt atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa
    43921 caagctaaag agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt
    43981 ggattgtcct taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt
    44041 cgagctttga ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt
    44101 caattgctga tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag
    44161 actcttatgg tcggagtttc tgaggaattg aagacacttg atttaacgga tatagcaggg
    44221 acttgctcct atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct
    44281 atgaaagctc tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct
    44341 catgtcttga ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa
    44401 ttgctgaaat aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga
    44461 aatgaataaa cgtctatttt taaaaatgag tctggtgacg ttgccaattt tagccttgtt
    44521 ttcacaacca gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc
    44581 gaatggctat tcggatattc acgaggggga acctggttat tctgccaagt tagaccgtga
    44641 tcatgatggt gtggcttgcg aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca
    44701 gtcaacggct attcaaatca acacaagttc agcaacaaca agtggttggg ttaagcagga
    44761 cggcgcttgg tactactttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaag
    44821 ctattacctg aaagctgatg gtaaaatggc acagagtgaa tggatttatg actcttctta
    44881 tcaagcttgg tattatttga aatcagatgg ttcttatgca aaaaatgcat ggcaaggagc
    44941 ttattacctt aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta
    45001 ccaagcatgg tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa
    45061 ctactatttg aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta
    45121 tcaagcttgg tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa
    45181 ttactatcta aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta
    45241 ttatgttggc gctgacggag tttggaagga aggtcaagca agtacagctt cttctagtaa
    45301 tgatagcaat agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt
    45361 ccacatgtca aaaaaaacgt atgtatagac aattaacttc tgattttgat aaattttcaa
    45421 atgatgcagc tcaatatgcc attgatcatt agatgattaa cagatactgg taggcgaaaa
    45481 aattccttat aattcactta cgcaaacctt tgcataagtg aatggatttt gatatactag
    45541 tactgtaagc attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat
    45601 tattgggatt gaccttggtg gaacttctat caaatttgca atcttaacaa cagcaggaga
    45661 aatccaagga aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga
    45721 tgatatgatt gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt
    45781 ccaaggcatt ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg
    45841 tgcctacaac ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcctt
    45901 gggcattcca tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat
    45961 gggtgctgga gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg
    46021 tggtattgtc gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg
    46081 tcatatcact gttgattttg accaaccaat tgcatgtact tgtggtaaaa aaggctgcct
    46141 tgagacagtt gcttcagcaa caggaattgt caacttgact cgtcgctatg ccgatgaata
    46201 cgaaggcgat gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac
    46261 tgtctttgat ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc
    46321 acgttacttg ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt
    46381 catcggtggt ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagtcta
    46441 tgatgaaaat actttcccac aagtacgcac atctactaaa ttagctcttg caactctagg
    46501 aaatgacgct ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg
    46561 aaaaatgcta acctagagtt agtaatgttc ctcttttatt ttttatgcta tactagttag
    46621 gacaatgaat gaggtgagag taaatgacaa aagcagatac gatttttaaa gagaatattg
    46681 aacgcattct aaaggaaggt gttttctcag agcaggctcg tcctaagtac aaggatggga
    46741 ctgttgctaa ttccaagtat gtaactggtg ccttttccga gtacgactta tctaaaggag
    46801 aatttcctat cacaaccttg cgtcccattg caatcaaatc cgccatcaaa gaagttctct
    46861 ggatttacca agaccagtca aatagcttag aagtgcttaa tgacaagtac aatgttcact
    46921 actggaatga ctgggaagtt ggagacacgg gaaccattgg tgagcgctat ggtgccgttg
    46981 ttaagaaaca cgacattatc aataagcttc tcaaacagtt ggaaaccaat ccttggaatc
    47041 gccgcaatat tatttcgctc tgggattacc aagctttcga agaaacagat gggctgctcc
    47101 cgtgcgcctt tcagaccatg tttgatgtcc ggcgtgttga tggggaaatc tatctggatg
    47161 cgaccttgac ccagcgctcc aatgatatgc tggtggccca ccacatcaac gctatgcagt
    47221 atgtggcttt acagatgatg attgccaaac attttggttg gaaggttggg aagttcttct
    47281 atttcatcaa caacctccat atctatgata atcaatttga acaagctcag gaattgctcc
    47341 gtcgggagcc gtcaaactgc caaccacgct tggttttaaa tgttcctgat gggactaatt
    47401 tctttgatat caaagcagaa gattttgagt tggtggatta tgaccctgtt aagccacagt
    47461 tgaagtttga cctagctatt taaaagaata gaaaaaagaa gttgagaata atcccaactt
    47521 cttttgtttc ttaacgtgat acgcggcgac gagctgcttt tttacgattt tcttcgatga
    47581 aagctgcttt ttgctcttct ggttcgatta ctttcttttt aaatgcgtat actgcacctg
    47641 caacggcagc gacagttcct gcgacacctg ttacaagacc tttagcgaat cctttagcca
    47701 tgagtcttcc tcctttatat tctcaatcag ccagcctcct caagaggtca catttttctg
    47761 actgaccttt ttgtgttata ataatagtaa cgaaaaaatg ggaatttttc aaggaaaaaa
    47821 gatgaaaaca aaaataattg tgattgttgg accgactgct gttggaaaga cggctctagc
    47881 tattgaagtt gcaaagcgct ttaatggcga agtggttagt ggagatagcc agcaagtcta
    47941 tcgaggactt gatattggga cggccaaggc tagtccagaa gagcaggcag ctgttcctca
    48001 tcatttaatc gatgttagag agataaccga gtcttactcg gcttttgatt ttgtttcaga
    48061 agctaagatg actattgagg atattcagag ccgtggcaag ctagccatta tcgccggtgg
    48121 gactggactt tatatccaga gcttgctaga aggttaccac ctaggtgggg agactcctta
    48181 tgaggagatt ttagcttatc gagctagttt ggagccatat tcagatgagg aattagccca
    48241 tttggtggag caagcaggcc ttgagattcc ccagtttaat cgtcgtcgtg ctatgcgtgc
    48301 cttagaaatt gcccattttg gtcaggattt ggaaaatcaa gagatcttgt atgaaccgct
    48361 gattatctgc ttggatgatg aacgtagtca actttatgag cgtatcaacc accgagtgga
    48421 tttgatgttt gaggctgggc ttttggatga ggccaagtgg ctgtttgacc attcccctaa
    48481 tgtacaggct gctaaaggaa ttggctataa ggaactcttt ccttatttcc gtggagagca
    48541 gacctttgag gaagctcgtg agagtcttaa acaggcgacc cgtcgttttg ccaagcgtca
    48601 gctgacctgg ttccgtaatc gcatgcaggt caccttttat cagattggag aatctggtgt
    48661 acaagaccgc attttaagcc agatagagga gtttttagat gattgaaacg gagaaaaaag
    48721 aggagcgagt cctgctgatt ggtgtggaat tgcagggtat ggacagtttt gacctctcca
    48781 tggaagaatt ggctagttta gcgaaaacgg caggggcagt cgttgtagat agctacagac
    48841 aaaaacgtga aaaatatgat tccaagacct tcgtcggctc tggtaagttg gaagagattg
    48901 cgcttatggt ggatgcagaa gaaatcacta ctgtcatcgt caacaaccgt ctgaccccaa
    48961 ggcagaatgt caatctagag gaagttctcg gtgttaaggt cattgaccgt atgcagttga
    49021 ttttggatat ctttgccatg cgggctcgaa gccatgaagg gaagctccaa gtccacctag
    49081 cccaactcaa atacctcttg cctcgcttgg ttggtcaggg gattatgctc agccgtcagg
    49141 cagggggaat tggttcccgt ggtcctggtg aaagccagct ggagctgaac cgtcgtagcg
    49201 ttcgcaatca aatcacggat atcgagcgcc agctcaaggt ggttgagaaa aatcgggcga
    49261 ctgtcagaga aaaacgtttg gagtctagca cctttaagat tggtttgatt ggttatacca
    49321 atgctgggaa atcaactatc atgaacatct tgaccagtaa gacccagtat gaagcagatg
    49381 agctctttgc gactctggat gcgacaacca agagtattca tctgggaggc aatctccaag
    49441 taactttgac agataccgtt ggctttattc aagatttgcc gacagagttg gtgtccagtt
    49501 tcaagtcaac cttggaagaa agcaagcatg tggaccttct ggttcatgtt atcgatgcta
    49561 gcaatcctta ccacgaggag catgaaaaaa cggttctctc catcatgaaa gacctggaca
    49621 tggaagatat tcctcacttg acgctttata ataaagcgga tttggtggag gatttcacgc
    49681 ctacccaaac gccatatacc ctcatttctg ccaagtctga ggacagtcgt gaaaacttgc
    49741 aagcattatt gctagataag attaaggaaa tttttgaagc atttaccctg cgagtgcctt
    49801 tttcaaagtc ctacaagatt catgatttag agagtgttgc aattctggaa gaacgtgatt
    49861 atcaggaaga cggcgaagtg attacaggct acatttccga gaaaaataaa tggaggttag
    49921 aagaatttta tgactgatat taaaactttg gctctaaagt atggaggtta tacaagtctg
    49981 gacaaggtct atctggatca gcttctagct ggtaaaacag agcaggagca gttaacactt
    50041 attacacctc cgccaagtgt tgttaatgcc tactttgcag aactctatca gaaaaaaagt
    50101 cctcaggctg cgacggatta ttttgcagaa ctcagtcagg aactgaatct ttacaatact
    50161 gagccaagtt tcaccctaga aaataagcct tttattcggc ttaatctgtc tggtaaatcc
    50221 tttggttttt gttatgagag tgacggcttt ggtcggattt tctctgaaaa tgaggagaaa
    50281 atctcggatg acttgctctt tgaaattgcg caaattttct cccatcaatt agtctttgaa
    50341 gagtctggta agatttacat gaagcctgtc aaggacgagg aagttgtcag cctagaaagt
    50401 ctcacagctt tgactgattt ggaaagattg gctgatggtc gaaaacgtct caaaggctac
    50461 agccaagagg atttattgca agaagctgct gctttttctg gcaaacgcta tttccgatcg
    50521 gaaaaccgca cagccatgtt atatattgat taattagaaa gtatcgaatg gatattcaat
    50581 ttttaggaac gggggctggt cagccctcta aagcccgcaa cgtttcaagt ctcgccctga
    50641 aacttttgga cgagattaac gaagtttggc tctttgactg tggagaaggt acgcaaaatc
    50701 gcattctgga aaccacaatt cgaccacgta aggtcagcaa aatctttatt acccatctgc
    50761 atggagacca catttttggt ttgccaggtt tcctttctag ccgtgccttt caggccaatg
    50821 aagagcagac agatttggaa atctacggac ctcaaggaat caagtcattt gtcttaacca
    50881 gccttcgtgt gtcaggttct cgtctgccct accgcattca tttccatgag tttgaccaag
    50941 attctctggg taaaattctt gaaaccgata aattcactgt gtatgcagag gagctggacc
    51001 acactatttt ctgtgttggc tatcgtgtca tgcaaaagga tctagaaggg acgctggatg
    51061 ctgaaaaact caaggctgct ggtgttccgt tcggcccgct ttttggtaaa atcaaaaacg
    51121 gccaggatct tgttttggaa gacggaactg aaatcaaggc agcagactat atctcagcgc
    51181 cacgtccagg taagattatc actattttag gagacactcg aaaaacggat gccagtgtgc
    51241 gtctggctgt caatgcagat gtcctagttc atgagtccac ttatggcaag ggtgatgaaa
    51301 aaattgctcg taaccatggt cactcaacta atatgcaagc tgcacaagta gcggtagaag
    51361 caggtgccaa acgcctccta ctcaaccata tcagtgcccg tttcctctca aaagatataa
    51421 gcaaactcaa gaaggacgct gccacaattt ttgaaaatgt ccatgtggtc aaagacttgg
    51481 aagaagtgga aatctagcag tcacagaaag gataagtatg cctactattc tcattaccgg
    51541 agctagcggt ggtctagctc aagaaatggt caaactcctg cccaatgacc aactcatctt
    51601 gcttggtaga aataaggaaa aattagccca actctacgga aattattccc atgcagaatt
    51661 gattgaaatt gatattaccg acgattcagc cctagaagct ctggtaactg atctttatct
    51721 ccgctatggc aagattgatg tcttgattaa caacgctggt tacgggattt ttgagggatt
    51781 tgaccagatt gctgataaag atattcacca gatgtttgag gtcaatacct ttgccctgat
    51841 gaatctgtct cgtcaccttg cggctcgtat gaaggaaagc agcaaagggc atatcatcaa
    51901 catcgtcagc atggcaggtc taatagctac tggcaagtct agtctttact cagcgaccaa
    51961 gtttgcggct attggttttt caaatgctct gcgactcgaa cttatgccct atggagtcta
    52021 tgtgacaaca gtcaatccag gtccaatccg aacaggattt tttgaccaag ctgacccaga
    52081 tggaacttat cttaaatcgg ttgaccgctt cctcttagag gcagatgcag tggctaaaaa
    52141 gattgtcaag attataggca aaaataaacg agaactcaat ctcccgattt tgttgaacct
    52201 agcccataag ttttatactc tctttcccaa gctagctgat aagttggcag gggaaacttt
    52261 taattataag taaaaagaac caatgtgcag gttgttgcta gcctacatat tggttcttat
    52321 catttctcaa ctatcaaact gaacttcttc tagtagatag tcgataaagc gttcgcccat
    52381 cttagatagg ctggttttct catgctggat atagaccagc tcaatcgggt catcaatatc
    52441 cagtgggata gaaacgatat tgtctccgtt taggttgctg ttcaaaatcc ctgtcgcaat
    52501 ggtataacca tccaaaccaa tcaagagatt aaagagggtg gcacggtcac tgaccacaat
    52561 ggatttcttg tggtgttcct gagaaagaat ctcttctgaa aagtagaagg agttgtgcgt
    52621 cccttggtca tagctgaggt aagggaaatt ctccaaatca gacagtttca ccttgtcttt
    52681 ctttgccaga gggttggtct tgctgacaaa gatatgcggt tgcgctgtga agagatggtg
    52741 ggctagcagg tgattgtcat ccagcatctt ggttaaaaca tcacggttgt aactgtttaa
    52801 gaagaggacc ccgacctcac tgcggaagtt cttgacgtcg tcgataatct cccaagtccg
    52861 agtttcacga aggaagagtt cgtatttctc catatcgctt ttcttgagca aagagacaaa
    52921 ggcattgacc acaaaggcat agtgttgaga cgaaacgcta aagagttcgc ggtgggcgac
    52981 aggattttta tagcgttcct ccagaagctg ggtctgctcg acaacctgac gggcataaga
    53041 gagaaactcc atcccatcac gggtcaaggt gattcccttg ggattgcgga taaagatctc
    53101 aatgcccatt tcattttcca aatctcgcac tgcattggag agacttggct gagtgataaa
    53161 gagttgcttg gctgcctcat tcatggagcc agtttcgacg attttgataa tataatgtaa
    53221 ttgttgaatt ctcatgtttt tattgtacca tacttgcctt agaatcgcta aagaagatag
    53281 gaaaataagg agcattgtat gagtctcttc ttgaaaaaaa cacaaaattt tgctagaatg
    53341 agtcttatga aaacattcta tgatgtgcag caattcctca aacgatttgg tattattgtt
    53401 tacatgggaa aacgcttata tgatattgaa ctgatgaagt tggaactatc tcggatttac
    53461 gatgcagggt tgatggataa attagactat ctagaggcag aagcagttct tcgcagagag
    53521 cacaaggtag aattggatta tattgagaaa aatggagaaa agaactaatg gttacttgga
    53581 ttttgtgggc acttatacta gcaatgttgg cgtggatggg ctttaactat cttcgtattc
    53641 gccgtgcggc taaaattgtg gacaataagg agtttgaagc cttgattcgt acgggtcaat
    53701 tgattgattt gcgcgaccca gcagaattcc acagaaaaca tatccttggt gcacgcaata
    53761 ttccttcaag tcagttgaaa actagtcttg cagcacttcg taaagataaa cctgtccttc
    53821 tctacgaaaa ccaacgtgcg caacgagtta caaatgcagc tctttacttg aaaaaacaag
    53881 gtttttctga gatttatatc ctttcttatg gcttggattc ttggaaaggg aaagtgaaga
    53941 ctagctaacc ttatttccaa attagtcgaa tgataggatg aagctattgt tttatagtat
    54001 aattaatttg tataaaattt aaagagttct tagaacatga aagaaaagaa gaaaaatcca
    54061 ctttttgtgg gtatcttgtc gattattctt ggtttgttat ttccaattgt aggcctgatt
    54121 ttgggtatca taggattggt cttggctatt tcataccaaa aagagtcaca attagactat
    54181 aaaatagaaa agattcttaa tatcttagga attgtgattt ctgtagttaa ctggattgta
    54241 gcaatcgcct taatttttcg gtaaataaaa gaaaaaagct ttagttttaa agcttttttt
    54301 attttatcga ctcatctcta aatagttttt aatgatttgt aactcttttt ggttcaaagg
    54361 gcggtagtta ccttctgcta aatctgggtt caatgtgaag tccccaaatt ggattctctt
    54421 gaggctagtc accttaacac caaccgagag gaacattttc ttgatttgat gaaattttcc
    54481 ttctgaaatg gtgatagagg cttgactgag ggaaggactt gctgatagaa tctctagtct
    54541 tgcaggttta cagatagtgc catctaaaaa gacaattcct ttttgaaagg tttggatatg
    54601 gtcaggtgtt agaagtccat taacctcaac ttgataagtc ttatcgacat gatattgggg
    54661 atggaggagt tggaagccca agggaccgtt atcggtcaag agaaggagtc ctgttgtatc
    54721 tcggtccagt cggccgacgg catagagctt gtcagactgg atgtcagatg gaagcaagtc
    54781 catgacggtc ggaagttcct tgtctttgtt ggctgtaacg acaccagcag gtttatgaag
    54841 cataagatag gtgtgttcat agccttgaat gattcgatcc tgaaaaagga gttcttgtag
    54901 tcctgtatcg atattttgag ctagggagcg ggctgggcaa ccatcgacta gaatgtcccc
    54961 tttgagtaag gcttgtttca tggcctttcg gctgattttt tcttgagcta ataaattatc
    55021 taaacgcata ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc ttgacgtgaa
    55081 aagaaaagtg gagtgaaaac atttacactt gcttgagtta tgttatttac ttgaaattat
    55141 ggtataatcg ttcagttaga aaataaattt tgaatattat agaggaaatc atgacaaaat
    55201 taagagaaga tatccgtaac attgcgatta tcgcccacgt tgaccacggt aaaacaaccc
    55261 tggttgacga attattgaaa caatcagaaa cgcttgatgc acgtactgaa ttggcagagc
    55321 gtgctatgga ctcaaacgat atcgaaaaag agcgtggaat taccatcctt gctaaaaata
    55381 ctgccgttgc ttacaacgga actcgtatca acattatgga cacaccagga cacgcggact
    55441 tcggtggaga agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg gtcgtagatg
    55501 cctatgaagg aaccatgcca caaactcgtt tcgtattgaa aaaagccttg gaacaagacc
    55561 ttgtcccaat cgtggttgtt aacaaaatcg ataagccatc agctcgtcca gcagaagtag
    55621 tggatgaagt cttggaactt ttcatcgagc ttggtgcaga tgacgaccag cttgatttcc
    55681 cagtggttta tgcttcagcg atcaacggaa cttcttcatt gtcagatgat ccagctgacc
    55741 aagaagcgac tatggcacca atctttgaca cgattatcga ccatatccca gctccagtag
    55801 ataactcaga tgagcctttg cagttccaag tgtcactttt ggactacaat gacttcgttg
    55861 gacgtatcgg tatcggtcgt gtcttccgtg gtacagttaa ggttggggac caagttaccc
    55921 tttctaaact tgacggtaca actaaaaact tccgtgttac aaaactcttc ggtttctttg
    55981 gtttggaacg tcgtgaaatc caagaagcca aagcgggtga cttgattgcc gtttcaggta
    56041 tggaagacat ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa gctcttccaa
    56101 tcctacacat cgatgagcca actcttcaaa tgactttctt ggtcaacaac tcaccatttg
    56161 ctggtaaaga aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg caggcagaat
    56221 tgcaaacaga cgtttccctt cgtgttgacc caactgattc accagataaa tggactgttt
    56281 caggacgtgg agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt gagggctatg
    56341 aacttcaagt atctcgtcca gaagttatcg taaaagaaat cgacggtgtt aaatgtgaac
    56401 catttgaacg tgtacaaatc gacactccag aagaatacca agggtctgtt atccaaagcc
    56461 tttctgaacg taaaggtgaa atgttggata tgatttcaac tggtaatggt caaactcgtt
    56521 tggtcttcct tgttccagcg cgtggtttga ttggatactc aactgagttc ttgtcaatga
    56581 ctcgtggtta cggtatcatg aaccatacct tcgaccagta cttgccattg attccagggg
    56641 aaattggtgg acgtcaccgt ggtgcccttg tttctatcga tgctggtaag gctacaactt
    56701 actcaatcat gtctatcgaa gaacgtggta cgatctttgt caacccaggt actgaggttt
    56761 atgaaggaat gatcatcggc gaaaactctc gtgaaaatga cttgacagtt aacatcacga
    56821 aggcaaaaca aatgaccaac gttcgttcag ctactaagga ccaaacagct gttatcaaga
    56881 cgcctcgtat cttgacactt gaagagtctc ttgagttctt gaacgacgat gagtacatgg
    56941 aagtaacgcc tgagtctatc cgtttgcgta aacaaatcct taacaaagca gagcgtgaga
    57001 aagctaacaa gaagaaaaaa tcagctgaat aagagctaga aagagagaaa gatggtctat
    57061 ttagtcctag gaattttact gctcctactc tatgtatttg cgacaccaga aagcattaaa
    57121 gggacagtca atatcgtcgc tatggtatgt attttagtgg cactcttgat tttattggtt
    57181 ctatcttttc tgaaaatttt tcaattacca acagaaatat tcctagcaat agccatgttg
    57241 atcctagctt actttagtgt gagagacatc acactcatgc cagtcaaaaa aagtaaaaga
    57301 agataaaaaa gagagttttg gctctctttt ttgctaggaa ttttacaaga ttgcaattta
    57361 tttttgagtt atcaccatct cttttgtttg gataatatga atgggaaaaa cttgaataaa
    57421 attctttaat ggaatctatt ttatatttgc ttagtactgg tt
//
LOCUS       DAVLTM010000003        55705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLTM010000003 DAVLTM010000000
VERSION     DAVLTM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.003"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(146..823)
                     /locus_tag="VYN16_000112"
     CDS             complement(146..823)
                     /locus_tag="VYN16_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1402705.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLASLVLGFF
                     KKKEDEK"
     gene            complement(781..1860)
                     /locus_tag="VYN16_000113"
     CDS             complement(781..1860)
                     /locus_tag="VYN16_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1402706.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDIVEPSRTVVEKIIPKLNIPEEEKKKKTSELAE
                     LISENVKVCYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNVTDKKLSSMT
                     TTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKEGSQEKL
                     AESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAYQKTVQQ
                     LTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(1971..2225)
                     /locus_tag="VYN16_000114"
     CDS             complement(1971..2225)
                     /locus_tag="VYN16_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402707.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPIWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(2565..3098)
                     /locus_tag="VYN16_000115"
     CDS             complement(2565..3098)
                     /locus_tag="VYN16_000115"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1402708.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKNLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(3085..3300)
                     /locus_tag="VYN16_000116"
     CDS             complement(3085..3300)
                     /locus_tag="VYN16_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1402709.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(3267..3410)
                     /locus_tag="VYN16_000117"
     CDS             complement(3267..3410)
                     /locus_tag="VYN16_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1402710.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            3644..5362
                     /locus_tag="VYN16_000118"
     CDS             3644..5362
                     /locus_tag="VYN16_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1402711.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNETAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            5473..5820
                     /locus_tag="VYN16_000119"
     CDS             5473..5820
                     /locus_tag="VYN16_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1402712.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(6098..6934)
                     /locus_tag="VYN16_000120"
     CDS             complement(6098..6934)
                     /locus_tag="VYN16_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1402713.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(6947..7576)
                     /locus_tag="VYN16_000121"
     CDS             complement(6947..7576)
                     /locus_tag="VYN16_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1402714.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(7586..8227)
                     /locus_tag="VYN16_000122"
     CDS             complement(7586..8227)
                     /locus_tag="VYN16_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1402715.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(8373..9602)
                     /locus_tag="VYN16_000123"
     CDS             complement(8373..9602)
                     /locus_tag="VYN16_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1402716.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(9592..10758)
                     /locus_tag="VYN16_000124"
     CDS             complement(9592..10758)
                     /locus_tag="VYN16_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1402717.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            10902..11915
                     /locus_tag="VYN16_000125"
     CDS             10902..11915
                     /locus_tag="VYN16_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1402718.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(11960..12379)
                     /locus_tag="VYN16_000126"
     CDS             complement(11960..12379)
                     /locus_tag="VYN16_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1402719.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(12390..13796)
                     /gene="atpD"
                     /locus_tag="VYN16_000127"
     CDS             complement(12390..13796)
                     /gene="atpD"
                     /locus_tag="VYN16_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1402720.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(13882..14760)
                     /locus_tag="VYN16_000128"
     CDS             complement(13882..14760)
                     /locus_tag="VYN16_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1402721.1"
                     /translation="MAVSLNDIKTKIASTKNMSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(14776..16281)
                     /locus_tag="VYN16_000129"
     CDS             complement(14776..16281)
                     /locus_tag="VYN16_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1402722.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(16296..16832)
                     /locus_tag="VYN16_000130"
     CDS             complement(16296..16832)
                     /locus_tag="VYN16_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1402723.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(16832..17326)
                     /gene="atpF"
                     /locus_tag="VYN16_000131"
     CDS             complement(16832..17326)
                     /gene="atpF"
                     /locus_tag="VYN16_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1402724.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(17340..18056)
                     /gene="atpB"
                     /locus_tag="VYN16_000132"
     CDS             complement(17340..18056)
                     /gene="atpB"
                     /locus_tag="VYN16_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1402725.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLPYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(18091..18291)
                     /locus_tag="VYN16_000133"
     CDS             complement(18091..18291)
                     /locus_tag="VYN16_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1402726.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(18366..19373)
                     /locus_tag="VYN16_000134"
     CDS             complement(18366..19373)
                     /locus_tag="VYN16_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1402727.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSVTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYAFWERGTNENHNRFIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            complement(19831..20229)
                     /locus_tag="VYN16_000135"
     CDS             complement(19831..20229)
                     /locus_tag="VYN16_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1402728.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            20357..20434
                     /locus_tag="VYN16_000136"
                     /pseudo
     CDS             20357..20434
                     /locus_tag="VYN16_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20602..21084)
                     /gene="greA"
                     /locus_tag="VYN16_000137"
     CDS             complement(20602..21084)
                     /gene="greA"
                     /locus_tag="VYN16_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1402729.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(21155..22810)
                     /gene="mltG"
                     /locus_tag="VYN16_000138"
     CDS             complement(21155..22810)
                     /gene="mltG"
                     /locus_tag="VYN16_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1402730.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADESQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATEIVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(22891..23385)
                     /locus_tag="VYN16_000139"
     CDS             complement(22891..23385)
                     /locus_tag="VYN16_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1402731.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(23382..23846)
                     /locus_tag="VYN16_000140"
     CDS             complement(23382..23846)
                     /locus_tag="VYN16_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1402732.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(23856..25190)
                     /locus_tag="VYN16_000141"
     CDS             complement(23856..25190)
                     /locus_tag="VYN16_000141"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1402733.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(25201..25818)
                     /locus_tag="VYN16_000142"
     CDS             complement(25201..25818)
                     /locus_tag="VYN16_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1402734.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(25866..28964)
                     /locus_tag="VYN16_000143"
     CDS             complement(25866..28964)
                     /locus_tag="VYN16_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1402735.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(29057..30223)
                     /locus_tag="VYN16_000144"
     CDS             complement(29057..30223)
                     /locus_tag="VYN16_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1402736.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(30231..31325)
                     /locus_tag="VYN16_000145"
     CDS             complement(30231..31325)
                     /locus_tag="VYN16_000145"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1402737.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(31483..32925)
                     /locus_tag="VYN16_000146"
     CDS             complement(31483..32925)
                     /locus_tag="VYN16_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402738.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIART
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(32931..33164)
                     /locus_tag="VYN16_000147"
     CDS             complement(32931..33164)
                     /locus_tag="VYN16_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV1402739.1"
                     /translation="MKLKLLRVDTKVIMWSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            complement(33336..35297)
                     /locus_tag="VYN16_000148"
     CDS             complement(33336..35297)
                     /locus_tag="VYN16_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1402740.1"
                     /translation="MKKSKSKYLTLAGFVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQDALVRNFTDGAYSYARLYP
                     NSSSFEGIKEKYKDDIIYSMQDATSYYWNFNLDRQAYKFTSKTTDIEKKSTQEAVLNK
                     NFRQAINFAYDRTSYGAQTQGEDGATKILRNLVVPPTFVSIKGKDFGEVVASKMVNNY
                     GKEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDATVDQASKKGIQE
                     VSSMKQSIEAALGTENVVIDIQQLSTEDYDNSSYLAQTAIQKDYDLYNGGWSPDYLDP
                     STYLDIFSVKNGGVLQNLGLDPGEANDKAKAVGLDTYTQMLEEANKEQDPAKRYEKYA
                     DIQAWLIDSSLAIPNVSLGGTPSLRKTVPFSAPYSLAGNKGVESYKYLKVQDKIVTTD
                     EYAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(35498..37120)
                     /locus_tag="VYN16_000149"
     CDS             complement(35498..37120)
                     /locus_tag="VYN16_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1402741.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            37226..38671
                     /locus_tag="VYN16_000150"
     CDS             37226..38671
                     /locus_tag="VYN16_000150"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1402742.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKILAFTGTKGKTTAAYFAYNILSQRYPTALLSTMNTTLDGTTFFKSSFS
                     TPENIDLFDMMAQAVKNGRSHLVMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(38717..38911)
                     /locus_tag="VYN16_000151"
     CDS             complement(38717..38911)
                     /locus_tag="VYN16_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1402743.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(39012..39620)
                     /locus_tag="VYN16_000152"
     CDS             complement(39012..39620)
                     /locus_tag="VYN16_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1402744.1"
                     /translation="MFGELIRYLDQYEDVILREIKSQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            complement(39645..40157)
                     /locus_tag="VYN16_000153"
     CDS             complement(39645..40157)
                     /locus_tag="VYN16_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1402745.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            complement(40244..41179)
                     /locus_tag="VYN16_000154"
     CDS             complement(40244..41179)
                     /locus_tag="VYN16_000154"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1402746.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(41250..41549)
                     /locus_tag="VYN16_000155"
     CDS             complement(41250..41549)
                     /locus_tag="VYN16_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1402747.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(41539..42288)
                     /locus_tag="VYN16_000156"
     CDS             complement(41539..42288)
                     /locus_tag="VYN16_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1402748.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADVAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(42342..42701)
                     /locus_tag="VYN16_000157"
     CDS             complement(42342..42701)
                     /locus_tag="VYN16_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1402749.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            complement(42703..44103)
                     /locus_tag="VYN16_000158"
     CDS             complement(42703..44103)
                     /locus_tag="VYN16_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1402750.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIGKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(44398..44637)
                     /locus_tag="VYN16_000159"
     CDS             complement(44398..44637)
                     /locus_tag="VYN16_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1402751.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(44669..45139)
                     /gene="ssbA"
                     /locus_tag="VYN16_000160"
     CDS             complement(44669..45139)
                     /gene="ssbA"
                     /locus_tag="VYN16_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1402752.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(45151..45441)
                     /locus_tag="VYN16_000161"
     CDS             complement(45151..45441)
                     /locus_tag="VYN16_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1402753.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(45594..46940)
                     /gene="asnS"
                     /locus_tag="VYN16_000162"
     CDS             complement(45594..46940)
                     /gene="asnS"
                     /locus_tag="VYN16_000162"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1402754.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNRFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(47097..47321)
                     /locus_tag="VYN16_000163"
     CDS             complement(47097..47321)
                     /locus_tag="VYN16_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1402755.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            complement(47314..48501)
                     /locus_tag="VYN16_000164"
     CDS             complement(47314..48501)
                     /locus_tag="VYN16_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1402756.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEVA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEESEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(48498..48986)
                     /locus_tag="VYN16_000165"
     CDS             complement(48498..48986)
                     /locus_tag="VYN16_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1402757.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            49307..49855
                     /locus_tag="VYN16_000166"
     CDS             49307..49855
                     /locus_tag="VYN16_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1402758.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALASKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(50092..55692)
                     /locus_tag="VYN16_000167"
     CDS             complement(50092..55692)
                     /locus_tag="VYN16_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEV1402759.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKLAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDLDK
                     GYSGFRAAPAPTNKLVTNLGNNTVFTDISRGSHTFRGNGNSRGGNPIHFDVTTTRVGN
                     RVEFSIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSS
                     YSLTSAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKD
                     NKNDGTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNE
                     LDASDKKLKEVIIADGAKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPG
                     KDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSV
                     QNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPD
                     VTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEG
                     NIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEA
                     HQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDG
                     HSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENN
                     DGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPE
                     KPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAT
                     GQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSP
                     SRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDGGVDKVLNSKDIYNGIDGRDGSAPT
                     ITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGS
                     TKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTE
                     NPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTT
                     ETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTETIITDGKSPVATVRDNQDGTYTI
                     RVENGNGTVSETTVRDGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEV
                     IDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSII
                     KDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGD
                     GSHTITIINSDGTVTKTVIKDGKDGRDGRDGRDGKDGKDGKCGCQDKPVTPSNDKPVP
                     PTPNVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQ
                     SDYISVLLGSGILLSLYVGRRKED"
BASE COUNT        15901 a        12321 c        10221 g        17262 t
ORIGIN      
        1 aatattttta aaaaaatagg attcatagtt tcccagttca acgtacttgt aagcgttttt
       61 attcttcacg aaagacctcg ttctcaaaac caggaaaaaa gcccacatct gtgagctttt
      121 cctggttcct agattctaaa atctattatt tttcatcttc ttttttcttg aagaatccta
      181 atactagact agctaacaag gtcatgatac cagcactagc caaggtagca ttagcttctg
      241 tacctgtgtt tggcaattcc tctctcttac ctgtctcata agtcggaact tctgggtctg
      301 gattcactgg agtttcagtt tttggagtac ctggttctgg agttggctct ggtattggag
      361 tctcttgatt tggtagttct gggtttggtg tcggaacact tggatgctct ggatttggtt
      421 gtgggattgg tttttctgga gtcggttcac ctggagtacc tggttcctga ggacttggaa
      481 tttctggttt ttctggagtt ggtttatctg gtgttgataa acggtcatac cttaccgtta
      541 tttctttatc actagagtct gacgtaactt cttgtgattc aactgttgga atatctggat
      601 ctttgtactt gtcaatctta ccagatataa cctcgtccca gtttcctgtt gtccattcac
      661 cgtaggttac aactcccgtg accttgttct cagtttttgt acggcttaag gttacaggtt
      721 gaacaacatc ttcttttaca ttttggttcg taactttatc aacgtaatga atgatacgcg
      781 ttatagtctt cgtctcagta gaggttgctg ttttgggaac cactgtttcc tcaacattct
      841 cacggtagta atagtcaact gttgcaccgt cttctggtac gcatttgcag gagttgctac
      901 caaggtgtat gttgttttcc ttgtgataac tcggtcttct ttgtcctcag ttgttgtttt
      961 cccttcaata gtttttgatt ctgtggtata ctcagaacct atcgctaaat cagcttttat
     1021 aacagactct gccaacttct cttggctacc ttctttatag taattggctg ttactgtagc
     1081 agtggttggc gcttcgcttt ctctataaac taaggtcact gttctacctt cgcttacaat
     1141 attcccagtt aaacttgcag aatttgtatc tgcttcttta aaagtataat attttccgtc
     1201 agtagtagtc atgctactga gttttttatc tgtgacatta tagctggtac caatcagttg
     1261 ttttttattg gtaatgtaag ttccgtcact ttctttttct ccaattccag tatcattttt
     1321 caatgatagc aaacgccctt gttcatcaac atagcaaact ttcacatttt ctgagattag
     1381 ttctgccaat tctgaggttt ttttcttttt ctcttcttcg ggaatattta gttttggaat
     1441 gattttttca acaacggttc gtgatggttc cacaatatct tggatgactg aaaagtcagc
     1501 tagaattggg agattatact gaacacggtg actttgagtg tttactccta ctctttcatt
     1561 attctctgaa aatacttgta cggtataaga aacaacatct tttcctaata gaacatcccc
     1621 agtagagaaa tagccgcctt ttcctagttt gctatctcca gagtccactt ctttcctaat
     1681 cttatcagat agttttttac cagtcagtac attcgttcgc acaatccctt tgtctacccc
     1741 tacaaagtgg gagaactttt tgaactcttc agaaccagat ctagcccaac cattattaag
     1801 ggcatttgct tttgtatttg tattctctct caaaggtttg gcgattagaa ttatattcat
     1861 cggcacttag agttgctgct atatctgact cttgaatacc aacttcctta ctaccatttc
     1921 tagcggcagt atatgtgtat ttatctgttt ctacttctag ctgattatcc ttaacatgat
     1981 aggtaatttt ctcctggtat aagagtcttc agtagtttat atgctctgtc atcatcttta
     2041 gctttagaag cagctgagtt tagccaaatt ggggtattag gagtatcttt atcagcctgt
     2101 ttcaagacat tattcaagtc atttgctaac ctattggttg tcctcactgt gaaagttttc
     2161 ttccagtctg aaccactttt attagactta gatatgctca tgaaatcctg gtcaacattc
     2221 aacacaatat cgccttgttt tttagcatct tccgttgctt ctgcttcaaa ttgatcatgg
     2281 agtttttgtt gctctggtgt atagtttttt tatttacacg atttaatgta ctatttttta
     2341 ttttagtatt aagaactcca ttaatacgat ttttaaattc tgcttcaaaa aatcccttaa
     2401 tttgttggtg cgcaagttca acattcaaac ctttcttata caagtcttca ttctgaacga
     2461 tttcggctac acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct
     2521 tagttttaat gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa
     2581 aagcattcca aacattcaaa aatagttgga acttccctta ataaatccgt cactttcttt
     2641 ttcaagatag cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt
     2701 aagaaaaagt gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta
     2761 gcattgccca tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac
     2821 cactccacaa agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct
     2881 ccgtctactt gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca
     2941 tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct
     3001 gtttcgtcaa tatatacgat tggtaaattt tttaggttat ccaaaatatc aagaaactca
     3061 gcgactttct ctggctcttg ttccttaaag ctcgtcatct ttttttaaag tgaccttaat
     3121 ctgctttaaa gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg
     3181 taaaaaagca tctggatgag cctctacaaa ggctttcaat tcctctaaag gaatctttcg
     3241 gtttttgacg actcgctttt tcctctctat gtgtccttgt tcacatcttt cttttcccac
     3301 gtgaagagag ttctgacgcc aacaccaaaa aacttggctg cctcgacatg gctgtgcccc
     3361 tctttgatgt aatctaatgc tcgctgttta aaatcagtac tatatgccat agctttatta
     3421 taatatgctt gtttaaaact aagcaactat atcaatgttt tgttattaga agacttactt
     3481 tttttactga aatcgaattt ttcctaacct caacatcata taaccccaaa attcaatctg
     3541 acctggtttg aagagatgtt cgaagcagat aaggttttac taaaactaaa aaaaatgata
     3601 taatcaaact gattaaatgt tttaaaaata aaggagaatt tgaatgtctt accaagaaaa
     3661 ttaccagaaa tgggttgatt ttgtggagct tcctgactac cttcgtcaag atttggaaaa
     3721 tatggacgaa aaaactaagg aagatgcctt ctatacaaat cttgaatttg gtactgcagg
     3781 tatgcgtggc ttggttggtg ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc
     3841 tactgaagga ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt
     3901 agcaattgcc tacgatagcc gtcacttctc acctgagttt gcctttgaat ctgcggcagt
     3961 tcttgctaaa cacggcatca aatcttacgt atttgaaagc cttcgtccaa ctccagaact
     4021 atcatttgca gttcgtcatc tcaactgttt cgcaggtatc atggtcacag ccagccacaa
     4081 ccctgctcca tttaacggtt acaaggttta cggtgaagac ggtggacaaa tgcctccaca
     4141 cgatgcggac gctttgacta cttatatccg tgcaatcgaa aacccatttg cagttgaagt
     4201 tgctgatgtg gaaactgaaa aagcttctgg cttgattgaa gttatcggcg aagctgttga
     4261 catagaatac cttaaagagg ttaaggacat aaacatcaac ccagccttga ttgaagaatt
     4321 tggtaaagac atgaagattg tctacacacc acttcatggt actggtgaaa tgttggctcg
     4381 tcgtgctctt gcccaagcag gatttgactc tgttcaagtt gttgaagcgc aagcaactgc
     4441 tgacccagac ttctcaactg taacatctcc aaacccagaa agccaagcag cctttgccct
     4501 tgctgaagaa cttggtcgtc aagttggtgc agatgttctt gtcgcaactg acccagacgc
     4561 tgaccgtgtt ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa
     4621 ccaaatcggt gctatcatgg ctaaatacat cttggaagct cacaaaaacg ctggaactct
     4681 tcctgaaaat gccgctctct gcaaatctat cgtttcaact gacttggtaa cgaagattgc
     4741 tgaaagctac ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa
     4801 aatccaagaa ttcgaagaaa aacacaacca cacttacatg atgggatttg aagaaagctt
     4861 cggttacttg attaaaccat ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt
     4921 tgctgaactt gctgcctact accgttctcg tggtttgaca cttgctgacg gtatcgaaga
     4981 aatctataaa gaatatggct actacgcaga aaaaacaatc tctgttactc tttcaggtgt
     5041 cgatggtgct gaacaaatca aagcgattat ggctaaattc cgcaacaatg ctccaaaaga
     5101 atggaacgaa acagctatca ctgtcgtaga agacttcaag gcacaaactg ctactgttgc
     5161 tgacggtact gttacaaact tgacaactcc tccaagtgat gtgttgaaat acacacttgc
     5221 tgacggttca tggattgccg ttcgcccttc aggtacagaa ccaaaaatca agttctacat
     5281 tgcagttgta ggggaaacca acgaagaatc acaagctaag attgctaaca tcgaagcaga
     5341 aatcaatgca tttgtaaaat aaagacctat aaaagatgag accaaccaac ctcatctttt
     5401 ttcgtatcaa atctaagcaa ttttagaaac tttatggcat actagactta tcaatgaaag
     5461 cgaggtaatc tcatggaaca atttttagat aacatcaaag accttgaagt cactacagtt
     5521 gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa
     5581 acttgccctt actgccgtaa atttgcagat acattgtcag gtgtcgtagc tgaaaccaaa
     5641 gctcacattt acttcatcaa tagtgaagaa ccaagccaac tcaatgattt gcaagcattc
     5701 cgctcacgct atggaatccc aactgtacca ggttttgttc atattacaga tggacaaatc
     5761 aacgtccgtt gcgactcttc aatgtcagca caagaaatca aagatttcgc aggattgtaa
     5821 aaaatcagct aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg
     5881 atattatgcg ttttattgtt cctaaaacag ttacatcagc atttcattac agaacatttt
     5941 aattttggaa gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg gctattccat
     6001 attcaattat ccatattcaa tcgctattat cgttttccta gattacaaaa aaactacgtt
     6061 ttgctaatca aaacgcagtt tcagccacta ttttatctta ctgtccttct tttacttctt
     6121 tggttgctac atcttctcca aaccattttt ggctgatttc ttggaacttg ccgtccttgt
     6181 aaagactaga aaaagcttca tttatcttct taaccaaggt tgtatcttcc ttacgggctc
     6241 caaccgcaaa agcttctgtt tctagtccaa ctgtaaagac attataatcg tttaaaacac
     6301 cttctgcttc taaataatag tttgcataga cacggtcaat caatagacca tcaattcgat
     6361 cgtttttcaa atcaatcaag gcttcattaa aggtttggta ttgattcgct tccttattag
     6421 cgacaatatt cttcaaaatt tctggatttg cttcaaagtc cgcataacca gatgaaccag
     6481 cttgagctcc taatgtcttt ccagtcatat cctttgcagt cgtgatacca gatgatttct
     6541 tcgtaaccaa tacctgctca ttcttcatat atgagttact gaaagccacc ttttcacggc
     6601 gttcgtctgt agcggaatag ccattccaaa tcagatcaat cgttcctttt gtcaattcag
     6661 cttctttcaa atcccaatca atcggttgcc aatttaccgt gattccgtat ttttcaaaaa
     6721 cagctgtagc taaatcaata tcaaatcctg cataagaacc atctttctga gcaaatccca
     6781 ttggaacaaa agtactatca aatccaatag taatagactt gttagactgg tactttgacc
     6841 aattatctcc actagtttcg ctagaatttt tcccacaagc tactaagaac aaagcagtca
     6901 tcagactgac taatacaagc atccattttt tcatccattt ttcctcctat ttaggttcta
     6961 ctttcaataa tacatctgcg atattttcag caaactgcaa atcatgggta accacaatct
     7021 gggtcatccc aagttcccta ttttgcaaga ttagcttctc cacttccaaa cgtaattctg
     7081 gatccagggc agaagttggt tcatcgtagc caatgatttc tgggtcaatc atcatagcac
     7141 gcgccaaagc cacccgctgc ttttgcccac cagatagtga gaaaggatag gcctctgcgt
     7201 gtcctcctag tcctaactgt tccaagagtc cactcgcctt cttctcagcc tcttcctgct
     7261 tcattcccat ggtcttcaca ggcgataaag tcaaattttc cagaactgat agatgaggaa
     7321 atagttgaaa atcttggaag acaaatccca gtaggttgcg cttctgcaat tcatctagct
     7381 ctaaaggttg tccattataa aagatttgcc ctgaatcaat ggtttcaaga cctgcaagca
     7441 tacgtaaaag agttgtctta cctccaccag aaggtccaac gatagccagg atttgctttt
     7501 caggaatact tagactgaaa tcagacagga tttgtttgtc tccaaagact ttattgatat
     7561 ttcgtaattc taacatagct gccccctatc tataataact gtactttttc tcaacttttt
     7621 tggaaataat tgtcacaatc ccaattaaaa tcaagtaaat ggctcctgcc aagaacatag
     7681 gaaccagact agcatcgcgg ttagcagctg ttcgactagc caagataagg tctgaaattc
     7741 cgagagcata gaccaaagaa gtatccttga ccaaactcat aacttcatta aagacactag
     7801 gaagaacgat cttggtcact tggggcaaga taatatagcg cactctgtca aaagggctaa
     7861 acttcaagac cttggcagcc tcatactgtc ctcttggaat agtgtcaatt cccccacgga
     7921 aaatttctgc aaagtaagct gcatagttga gaacaaaggc aataatagct gcaggaaggc
     7981 ggtctaaacg aatcccaata cttgggagca cataatagat aaaaatcagt tgcaagagta
     8041 agggggttcc tcgcataacc cagatgtaaa tgttaatcag ataatggagg ggcttccaat
     8101 ggacttgcaa ggcaaaggca atcaaaacgc ccaagggaat cgaaaatagc aagaccagtg
     8161 caaagacctg tacagtcgtg ctcgcaccat tcagtaaact cggtaatatc tcaaataaat
     8221 aagacatact gcacctccta aaagtaattg ttcctattat agcatgaata aacaagatta
     8281 ccaagtattt ttgtaaaaag attctaccat atacaaataa aaaccgtagc tatcaaaaga
     8341 gataaactac agttctatat gtttaaacat tcttacaatc tctcaaacag ttcttgcatt
     8401 tgcacatcat ctggaaccag ttttaagtaa gtgtgagcat ggacttttgc ttcttcaaaa
     8461 tgtcccaatt cacgcaagag atagatatag tgttccagaa attctggatt gtccttcaaa
     8521 tctcctgtca actcttgata atgctcataa gcagtatcca aatcgtccat ttcttgataa
     8581 gaacgagcaa tcatccactt ggtcaaaaga ttttctggct cctcactctg caagtctaga
     8641 atatcctcat aacgctcctg ctccagataa atagtggcta aacgaagcaa gatttcttct
     8701 gtatcctcag cgtcttcttt tgcagtaagg agataatttt ctgcaccact agcatcatgc
     8761 aattcataag aaaattgtga agcagctagc aagaggcgag tttcaaaggg atttttctct
     8821 aatccttgct tagcgatacg cagggcttct tgaacttgat gttccttatg taaagcctga
     8881 ctgtacccat actcatagcc ttcaaagtca ggagaaatgg tatcaagctg cttaaagtag
     8941 agggtggctt tttgatattc ttcttgatca aaataaagac tggccaactc aaaagctgtt
     9001 aagtcatcgt attctaactc cagggctttt tctaaaaact cagtagccgt ttcaaatttc
     9061 cctaactgag cataggcaaa gccaattcgt tgataggtgg aaatgcccgt ttgctcataa
     9121 atcgagcgat tatctaactg ggcataggct tgaatagccg cttggtaatt ttccaactca
     9181 ctatccaact ctgccaaacc caatatcaag agagaatcct ctgagtaggt caaggcctcc
     9241 aataatttct cacgtgccac atctgtcaaa ccttccagct ggtaaaggtc tgccttcaga
     9301 gccaaagacg agacatacca gtcactgtca gcttggattt cctcaagata ggtaaaggct
     9361 tcttctattt gaccatcctc gctagcaatt gcagctagat taagatgaac ctctggaaaa
     9421 tcctctacaa ttttcaggta aatttccttg gcctgaggat agaaaccaat cccttcaaga
     9481 taagttgcca actcatacag aagatcactt gaatcatttt ctaaagcttt ggcgaaataa
     9541 tgctcagcct tagttaaatc ttgctcctcc aaagcctgta acatctgttg actattgttc
     9601 actcttgact tcctcaccct ctaattcata tagaccactg actaccttat accattcgaa
     9661 aatggctgaa atgacaacct tagcagaggc ataaaccgga ataccaagta aaactcccca
     9721 gataccaaac atagatcctg aagttaacaa aacaaagaga acattaatag ggtggatgtt
     9781 taattgactt cccaaaatca atggagagac aaaacggcct tcaatagttt gttctacgat
     9841 aaagacaatc actactttca aaagcatgac tggaccagca atcaaaccca atactagagc
     9901 aggaagcatg gctagaaagc taccaagata agggaccaga tttaaaatac cagcagtaac
     9961 ccccagcgta accgcatagc gtagaccaat aatcttgaag aagatgataa acattactgc
    10021 tacaataata gccactgtca cttgccctcg aacatagttg gacaactgtt gattcacatc
    10081 tgatagaact tgtccaacag gttccttcaa ttttcttgga atgaattggg tcaaatagtt
    10141 acgcaagcct ttcccatcac gcaagagata aaagagcatg aaaggaacga taatcaaggc
    10201 aacaatcact tgagaagccc cgctaataaa ggcactcacc cagttgactg cctgagatga
    10261 aaccttactt gccaaaactg tagcctggct agaaaaattg gtcaaaactt gctctaattg
    10321 aggtctgaaa tcatctggca gatgctgggc taccaatcca ttaacaatcc tatctatatc
    10381 ttctaagtaa acaggaacgt ttcttgcaaa ggtcaaaacc tgacgttgca gatttggaat
    10441 ggcgactgcc aagccccaaa tgataaagag agcgatgata acaaagacaa tagtgatagc
    10501 tataacacga ttaaccttat gcttctccat ccaatcaaca ataggattca acaaataata
    10561 taacaaacca gacaaaatga ctggcaacat cacaactgct aaaaagtcta aaacaggtga
    10621 aaatagaaaa ctaatcttac ttaaaataaa aagattcagt cccaataata aggttactaa
    10681 aaataccgta attgccttgt tatctaaaaa ccacttgaaa aaccaagata ggctaaaatg
    10741 tttctctttt tgctccataa atacttcctt tctattgttc tctcattata ccatttttga
    10801 aacaaaataa ctctttttta aagtgcttac atctaattgg atatctttaa tttacttgtt
    10861 tttgtgatat aatagtctta tctttacaca gaggtatagt aatgaaagaa actgtttatt
    10921 ttggaactta tacacgtcgt acttctcaag ggatttacaa ggcagacttt gatacagaaa
    10981 ctggtcagct ttcaaatcta gaactttttg cagctgagcc aagtccaacc taccttgcct
    11041 ttgaccagca ccaacattta tacactgttg gtagccaaga cgataagggg ggaattgcag
    11101 cctatcaaac tgacgggact gtgttaaatc atgttgttga agaaggagct ccccactgtt
    11161 atgttgctgt cgatgaaaag cgtgatttgg tttacgcagc taactatcac aagggacaag
    11221 tccttgttta taaacgccag gaagatggta gtcttctact tagtgatatg gatcaacaca
    11281 gtggccaagg tccacatgaa aatcaagctt ccccccatgt tcactataca gatttaacac
    11341 ctgaccacta tctagtgacc tgcgacttgg gaactgacca agtcatcacc tatgacctcg
    11401 atcaagaagg aaaattatct aagctctata cctatcacag caagccagga gcaggctcac
    11461 gccatatcat tttccataac cactataaaa tcgcttatct catttgtgaa ctcaatagta
    11521 ctatcgaagt tttaatctac gatggcgttg gcgaatttga acgtatgcag gtcatttcaa
    11581 ctttaccgga agcttacgaa ggctttaatg gaaccgctgc tattcatctc tctaaagacg
    11641 gtaaatacct ctacgcttct aaccgtggcc atgattctat cgcagtatat accatccttg
    11701 cggacggtag cttagagtta ttagaaatcg ttccaacgca tggtcagact ccacgtgatt
    11761 ttgatttgac acccgaccaa aaatttctca ttgttgtcca tcaagactct gacaatgcaa
    11821 ctgtctttaa acgtaattgt gacaatggtc gtctagcaga actctccaac gacttccatg
    11881 ttcccgaagc agtctgcatc cgttttgctc cttaaaacac cataaaaaat gaacttggta
    11941 ttttcaagtt cattttttct tatagtctat ttccgacatt aatacggtta atagcacgtt
    12001 gcaaagcaat cttagcacga cgttcttggt caatcaaatg tttgtcttgt gcttcttcaa
    12061 ttgcacgttc tgcacgaagt ttggcacgtt ctgcacgact gatatcgata tcacgagcac
    12121 gttctgcaga gtcagcgacg attgtgatca tatcattggc aatttcaata acgccaccgt
    12181 ttactgcaat ccagttcacg tgatctttat catcgatacg ttttaccttt acttcatcaa
    12241 ctgctaaaac cgcaatcata ttttcatgtc gtggcaagat ccccatctca ccatccagag
    12301 ttcgaaccga tacatagctg gcatggtgat catagacgag accatctggt gtcacgatct
    12361 ggacagttaa ctgagccata gatcacctct taaaatccca ttttttcagc ttttgcaatc
    12421 acatcttcga tagaacctac accacggaag gcatcttctg gcaagtggtc gtatttacca
    12481 tcaaggattt ccttaaagcc acgtacagtt tcagcaactg gaacataaga acctggctga
    12541 ccagtaaatt gttccgcaac gttgaagttt tgtgacaaga agaactggat acgacgggcg
    12601 cgagcaacca aggtcttttc ttcatcagaa agctcatcca taccaaggat agcaatgata
    12661 tcttgcaatt catggtaacg ttgaaggaca cgttttactt cagcagcaac tgcatagtgc
    12721 tcttctccaa cgatttcagg tgccaaggca cgtgagcttg aagcaagtgg gtcaacggct
    12781 gggtagatac ccaattgtac caacttacgt tccaagtttg ttgttgaatc caagtgagcg
    12841 aaggctgttg ctggcgctgg gtcagtatag tcatccgctg gcacatagat agcctggata
    12901 gaggttacag aacccttctt ggttgatgtg atacgttctt gcaattgacc catttccgta
    12961 gcaagtgttg gttggtaacc aacggctgat ggcatacgac ccaaaagggc agatacttct
    13021 gaaccagcct gagtgaaacg gaagatatta tcgataaaga gaagcacgtc ttggccttcc
    13081 acatcacgga agtattcagc gattgtcaaa ccagtaaggg caacacgcat acgtgctcct
    13141 ggtggctcat tcatctgacc aaagaccatg gctgttttct cgataacgcc tgattctttc
    13201 atttcccagt aaaggtcatt cccctcacga gtacgttccc caacaccagt aaatactgaa
    13261 ataccaccgt gctcttgggc aatgttgtga atcaattctt ggattaagac ggttttacca
    13321 actccggcac caccgaaaag tccaacttta ccacctttaa ggtaaggggc aagaaggtca
    13381 ataaccttga tccctgtttc aaggatttca gaagaggtag acaactcatc aaaagttgga
    13441 gcttttttat gaattggctg acgctctgcg tcttctgtaa aaggagcttc caaatcaatg
    13501 gtatctccca aaacgttgaa gacacgtccc aaagtttctt tacctactgg tacagagatt
    13561 ggacgacctg tgtccaatac ttccattcca cgagtcaacc catctgttga ttccatggcg
    13621 atagtacgaa ccataccatc tcctaactcc aaggctactt caaggacgat ttttgttttt
    13681 ctttcgtcat ttttgtagac gacaagtgca ttgttaatct caggaagttt ttcccctgct
    13741 gcaaacaaaa cgtctacaac gggaccgata acctgagcaa ttttacctga actcatctcc
    13801 ttctcctatt ctatataaga tactgtcggt tcctagctca actaggtcct aagttcattt
    13861 tcatacgagc tggactagag cctattctaa ggcactagca cctgctacga tttctgtaat
    13921 ttcttgtgta atcgccgcct gtctggcacg gttatactga attgtcaaat cattgatgac
    13981 tttcttagca ttatctgtcg ctgtttgcat ggctgtcata cccgcagcat tctcagctgt
    14041 cttggcatcg ataatggcac cgtaaatcat actttctgca aactgaggca acaactgctc
    14101 cagaatttct tctcggctgg tttccaattc aaaagtcaag ctgtactctt catccgcttc
    14161 atttggatcc aagtcaacaa tcggaagcat ttgttccaca cgcatttgac tggttagcgt
    14221 attgacatgg tggttgtagc aaacataaag ctcatcaaag agttcatttt ggtacatttc
    14281 aacagttttt gaaataatct tacgaacttg atcaaagcta ggttggtctg ccaagccacg
    14341 taattcataa agtggttgaa taccgcgagc cttaaagaaa tcagctccca tcccaccgat
    14401 acagatcatt tcaaaacctt taccgtctgg gtggtattct tctttcaact ccataacagc
    14461 tttcaaaata gaggaattat aacctccaac caaaccgcgg tctgaagtga taacgatata
    14521 gcctgtcttc ttcacagaac ggctaatcaa catcggatta gttgaagcac cagctccatt
    14581 accatgaagg atatctgtca aaagtttacg cactttctga gcgtaaactt ggaagttgcg
    14641 agcagcttct tcagaacgac ctagcttagc agccgatacc atttgcatgg cattagtgat
    14701 ttgactcata ttttttgttg aggcgatttt tgttttaata tcatttagag atactgccat
    14761 ctgacacctc tattcttatt ggaagctaga ttgattgaga aactctgtaa tcgcagcatc
    14821 caagactgct tcttctggca agtcttttgt atcacgaatg gtttccaaaa tctctggatg
    14881 ttgagcatca aagaaggtat ggaactcttc ctcgaaacga acaatatcat ctactggaac
    14941 agtatccaag aaaccatgtg tcaaagcata aagaatggtt acttgtttct caacaggtaa
    15001 tggtttgtga acaggttgtt tcaagacctc aacggtacga cgtccacggt tcaacttagc
    15061 ctgtgttgct gcgtccaagt cagaaccaaa cttagtaaag gcttccaact cacggtatga
    15121 agcaaggtcg atacgaagtg taccagcaac cttcttcatg gctttgattt gtgcagaacc
    15181 acctacacga gatacagatg aacccgcatc gatggctgga cgaatacctg cattgaagag
    15241 gccatcgcca aggaagattt gtccatcagt gatagaaatc acgttggttg cgatataggc
    15301 tgagatatct cctgcttgtg tctcgataaa tggtagggct gtaattgatc caccaccaag
    15361 ttcatcagaa actttagctg agcgctcaag caaacggctg tggagataga aaacatcccc
    15421 tgggaaggct tcacgacctg gaggacgacg aagcaagagc gatagttcac gataagctac
    15481 cgcttgtttt gatagatcat cgtatacaat caaaacatgc ttaccttgat acataaattc
    15541 ttccgccata gcaaccccag tataaggagc taggaagagc aatggagatg gttgtgaagc
    15601 agaggctgtc acaacgattg tgtagtccaa ggcaccgtac tgacgaagtg tttctacttg
    15661 cgtacgaact gttgattctt tttgtccaat cgcgacgtag atacagatca tatcttgatc
    15721 tttttggttc aagattgtat caatcgcaat ggttgttttc cctgtctgac ggtcaccgat
    15781 aatcaactca cgttgaccac gaccaatcgg tacaagggcg tcaatagctt tcaaaccagt
    15841 ttgcaatggt tctgaaacag acttacgttg cataacacca ggagctggtg cttctactgg
    15901 acgagtttta tcagtgtgga tttctccaag accgtcaact ggacgaccaa gcggatccac
    15961 aacacgacca atcagacttt cacctacagg gacttccatg attttccctg tacggcggat
    16021 tgtatcgcct tcacggatat ctgtaaagtc acctaggatg ataataccaa cgtctgttga
    16081 ctccaagttt tgagccatac cataagagcc gttttcaaaa ttcaacaact ctccactcat
    16141 gacattttca aggccatgag cacgcgcgat accgtccccg atataggtta caacacctgt
    16201 ttcagtcaca tcaaaattgg gtttgaaatt ttcaatttgt tgcttaatta aagcgctgat
    16261 ttcttgtgcg ttaattgcca aaagaacacc actttctatt tcaaattttc tttaacaact
    16321 ttaagttgtt gtttaatact cacatcaatt gtcttgtgat tggcaaaaat gacaaaacca
    16381 ccaatgagac tttcatcgat ttgttctttt acactcctta ctttcagaga catttttttc
    16441 tcaatcaaag ggagcaaacg agtcttctgt tcatcagtta gaggatgagc agacgtaatc
    16501 gtcacttcaa atcgatttgt ttctttttca agtcggttca agcaatctac aagcacatca
    16561 taaaaaagat ttgctctgtg attgtaggcc agaacctgga taaagttttg taataaaggc
    16621 gacacagaat cttggaaaaa agcaattgtt ttttccttat ccgactcgtc tactgccact
    16681 tgttttaaaa aagaaggcag acctgttttt tcaacaactt gcttgatttg agtcaagtct
    16741 gaaaagatac ggtcttcttc tcctttttca agtaccaatt ggacaaaagg catgctgtat
    16801 ttttcaatta cctttactgt tttcttgtcc attaagcttc tcctagctga tcgatatact
    16861 gatcaatgag tgctttatgg gcatgactgt caaggttttg tgagatgatt ttaccagcta
    16921 agctgatggt caaatctgcg acctcaccct taacactctg taaagcttct actttatttt
    16981 gagcaatttc ttgattggct ttttctttta agtgtcctgc ttctagttta gcatctgcta
    17041 agatattagc cttactttgc tcagctgttt cctttgcatt ttcaatgatt gtcttagctt
    17101 ctttacggct accagccaat tcatcttcgc gtttttgagc caatacttct gctttttgac
    17161 gggcttcttc agctctgtca atatctgaag caattttttc agctctttct tcgaaaatgc
    17221 ctgtaatatt agaccatgca aattttttaa tcaagactag caaaagaata aaagagccag
    17281 tgattaaaat aaaattacca attaattcac ctactgttac gtgcatcagt tactcctttc
    17341 tattcctctt caatattaat cttattccct aaatacacag atgtcaaaag agtaaaaaca
    17401 taagcttgga tgcaggaaat aaagacagaa aatgcagtcc aagccaaatt agctccaaag
    17461 gctactggat accaataaat agcttggtgg gaaagaagaa gtaacaaact cgtcatgacc
    17521 tctcctgcaa agatattccc aaaaatccgc aaagccaaag ataagaagtt tgtaaattct
    17581 tccaagatat tcatcggtat gacaaaaaca ggactcataa aagattttat acttttttta
    17641 aatccacgac gacgaacgct ttctatatgt gtcaacaaaa tgacaagaaa agataaggtt
    17701 aagtcatacg gtaaattagc ggttggcgaa ctccaccagt tagtcccatc gatcgtttga
    17761 agctttgtca ttaagccaag gttattggca atcaccatga aaaggaataa acaaaggaaa
    17821 aagagtgagt aatctttcat gtagcgcgaa ccaatgttag gttctgtaaa tccaataaca
    17881 aagtcataga catactcaag tacattttgc tttcctttgg gtttcaaggt catattgcgg
    17941 cttgcccaat aaataaagac aaaaataact cccacaatca acaaagtcat ggctaacata
    18001 gtcagattga agataacagg accaatagag atgattggat taatactttc ttccatctaa
    18061 aatcctccct tttcttctcg ttccatgtat ttatttgata ataaatgaga agacaagagt
    18121 tacaaagaaa gttccttcaa taaaggcaac acctaaaaac atcaaactac gaaactcaga
    18181 aagcatatct ggttggcgtg ctactgattt aaacagtcca ttcatcaata aaccttcacc
    18241 gacagatacg cccatacagg caatacataa gcctaaaaat gttaaattca taacaaattc
    18301 tcctttaaaa taaaatttac ttacttagtg tagtcctaaa atttaaattt gtcaactttt
    18361 aaccgctatt ggaagcgttt aataaaaatt atttccattt tgctaaattt caagttcaag
    18421 ttagccatcc agaagtcttc tctgggtgac ttgtagtcca agcatttttt aggatagttg
    18481 ttaatccact tttcgatgaa tgcgacttct ttgggagtca ttttcttggt tcccttaggt
    18541 aaccatctac gaatgaacct gttgtgattc tcattagttc ccctttccca aaaggcatag
    18601 gggtgcgcat aataaatgtg ctcctcagaa aatacatcaa acaagcgatt gaattccgtt
    18661 ccattatctg ccgtgatgga aagaatcttg tgttgtttta agatgagttt tagagcctga
    18721 ttgaccacct cagcactttt atttggaatc aatcggatga tctgatgtct actctttcga
    18781 tccgtcaaga caagcaagca gtagtttttc gctctcgtaa gtagaaccgt atcaatctca
    18841 taatgcccat tctccaagcg aagattgata gcttcagacc gctgttcgat ggattgacca
    18901 gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct agggtaaagc
    18961 agatcctgtt tgcttaaccc caattttcca tgatgaatcc aatagtaaat ggttgaaatt
    19021 cccacgttaa cccctttagc catcaccatc atttcaggcg aaaatttttg gttatgatag
    19081 tggagaatct tttcctttag ttccttggtc acgcttgatt tcttgaccga gcgcttgcga
    19141 ttgttttcat aagactgttg agcatagtcg gcagaataaa cctctttgaa gcgccctttt
    19201 ccaagacatt gtcggactgt cccacgcttg atttcagtgt ggatagtttg aggagctttt
    19261 ccaagtagag aggcaatttc tctatttgat ttcccttctt ttttccatct ttcgattaag
    19321 cgacggctat cgattgtcaa atgtttggct tttgtagtat aattgtcttg catttctgtg
    19381 cctttcttgt gtttgtggtt gaacaacaag tataacacag aggtgttttc ttatgcctac
    19441 aagagttttc attatttcca ttttctttcg gatttcactc tattctgaaa aacttgtgta
    19501 tatttactga agctagcaag tcttacctgt aaatttaatg aaagcaacac aaaatcctag
    19561 aggggaatct cggattaatg tcaatttaaa aagatagaga gtttttagtt taaataaatt
    19621 gttttaaaat atcaacaaca tcacttcttt tcttaacctg ataagccttg attcctaatt
    19681 ttggagctac gattatattg tcctcaatat cgtctagaaa gacacaattt ctaggttata
    19741 actggtattt atcgatagtt actcatatac atcagtacac ctccatactt atgtgcgagc
    19801 ctctctttgt attatacctc catactcacc ttacagattc ttttggtaat aatatctttg
    19861 cctaatgtag agatagtctt gcaaagaaaa aacttccttg tagccatgtt tctgataaaa
    19921 gtccggtgcc tggaactgat aagtattgac aaaggcaaaa caacaatttc gattcttagc
    19981 ttcactttct gcctgttgca atagttttga accgattcct tgccctcgca gttcctcttt
    20041 tacaaacaaa tactcgattt ctagccaatt tccaaaagtc tctgctatca aacccgcaag
    20101 gagattgccc ttttcatctt cgacataaag attaagtggc tcactttcag cctcttccct
    20161 ttttgaacgg ttataaacac gaatcagatt ccctatttct tgcgatttat gtgattcctt
    20221 attttccaat ctaaagtata atgaaatgaa ataaaacatg cgcaaatcga ttaaggaatt
    20281 taatctaatt tctaataatg tcttagaaat caaagtttac tattttaact tcaatgcact
    20341 atacatctaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctc
    20401 aaaacactgt tttgaggttg tagatagaac tgacgaagtc agctcaaaac atggttttga
    20461 ggttgtagat gaaactgaca aagtcagtaa ccatacatac ggcaaggcga cgctgacgtg
    20521 gtttgaagag attttcgaag aatagcaaaa tggaaaaagg agtgagtgaa gcacatcgcc
    20581 tccccactcc tttttctgtt tttaggctgt tttttcaacc ttcaagattt ttacatcata
    20641 gctaccaaca ggcgtttcaa tggttgctgt gtcacctgtt ttcttgccaa tcaaggcctg
    20701 cccaattggg ctttcatttg aaaccttacc tgcaaaggca tccgcaccag ctgaacctac
    20761 gataatataa acttcttctt cgtcctcacc aatttcttgg atggtgactg ttttaccaat
    20821 cgctacttcg tcctgggcaa ctgcgtcgct attgacgatt tcagcatagc ggatttttgt
    20881 ttctaagcta gagatttgtc cttcgacaaa ggcttgttca tccttagctg cttcgtactc
    20941 actgttttct gaaaggtcac cgtatgaacg ggcaatctta atgcgttcta ccacttctgg
    21001 tcgacgaacc aatttcaatt cttctaattc tttttcaagt ttttcctttt cctcaagggt
    21061 cataggatat gttttttctg ccatttttct caactttctt ctgataatat tttctaaaga
    21121 aaattatgtg acgtatcaca taattttagt ttgtttagtt taatttgctg ttgacatgtt
    21181 cagcgacatt gcggtcgtgg tcttcttgat tgttagcata gtagaccttg ccttctgtga
    21241 catctgctac aaagtagagg ttatcgctct tagtttgatt gatgcttgac tcaatcgcat
    21301 ccagacttgg actatcgact ggaccaggca tgagacctac atttttataa acattataag
    21361 gtgaatcaat gttggtatca atcgcaacat cctcagctag actgatattt tgccccagtt
    21421 ttccttgggc atacaagatt gcaatattac tttgaagtgg catatcacga tttaaacgat
    21481 tgtagaatac acctgcaatg agcttacgat cttctgtctt ggcaccttct ttttcgacca
    21541 aggaagcaat ggtcaacaac tcattgacag tcaaattttt agatttgata gtactatagt
    21601 aaggagatag gttcttatcc atagcagcta acatctcatc aatcaagctc tcaatagttg
    21661 tgctttcctt gatagagtat gtagctggga aaaggtatcc ttccaaacga taacgcgcac
    21721 cgctgtcttt tacaggcaaa ctttccagta aagtaggata tttcgctact gcttgactga
    21781 taaacgtctc atcttgaact ttagccaaga aagcctccgc tgtcaaagac tctttgaagt
    21841 caccttgcaa ttgacccaca gcttgagcaa tctgatccaa ggtataacct tctggaattg
    21901 tcaaagtcgc aagtacaggt tcttgcggtt catctgttcc acctttttgc aactctttga
    21961 gtaagtcttc tgtactcata ctcttttgca aattgtagta acctgctttc aagtcggtat
    22021 aatttttata cttggcataa aaactaaaaa tcagaccatg ctttaccaaa ccagcttttt
    22081 caagcgtcgt accgatttct tgaacgtttg aaccttctgg aattccaacc gtcacatatt
    22141 tcttagaatt agcatcgata ggtaacaaag aatctagcac gtactggtaa ccgaagtaac
    22201 cacctgctga gagcaaggca aggaatacaa ggaatgaaat cacaaaagct ttcacatgtg
    22261 acttcttctc tttcttaggc ttaacgggtt ttgctccttc tctacggcta cgacgtgatg
    22321 tatcactgat gatatccact atttctgtag caggcgtagg tgtttctgga cctgcttgtt
    22381 ctgctttttt ctctgccgtc gtataggaaa cagctaccct tgttggggtt tcattgtatt
    22441 ctctttcaag tttcttaggt ctaacaggac ctggacctgg tcttgatcca ctttcttccg
    22501 ctggagaaga aggtacatct tgacttggat gacttggaac accaggagtt tctctttgac
    22561 tctcatctgc tggagaagct ggtacacctt gacttgggtg agtaggcacg gtaggagctt
    22621 ttctcataat ctcctctacc gttgacaagg aatcagccat gagttcttca gctgaaggtt
    22681 catttgcagg agtgcgaact actgcctcat cttctttcag aacttcatca tagcctttta
    22741 ctttttctaa atctctcaga atctgctctt taaagcttaa tttctcttct tctcttgact
    22801 tttcactcaa aagtttttcc tccttgttga taatccataa tattatatct taaaagtcca
    22861 aaaaaagcaa tctatgatac ttttcctaac ttattttttc atttcccaga ccatatcata
    22921 ccatgtttcc cctgcaaagg ttgactggga ctggccttcg ttgacaaatc catgtttttc
    22981 ataataggcg atgagatagt catgacaggt tagattaatg ccatctctct catcttcaag
    23041 agcaacttct ttcaaggctg tcagcaattt ctgacccagt ccgagtccct gtgcctcctt
    23101 ggcaatagac agacaggtca cagagatata accaccaggc tcatgactat agtcttttat
    23161 ttcttctgta aaagactggt cttgcagatg gcggtgcagg ccaactggtc cttcgatata
    23221 acccatgatt cttccttctt tttcagcaac cagaaaagag gtctgaattt ctctcaaatg
    23281 tgcttcaaag acagaaggag gaatggcttc ttcgaccgaa aaattttcaa attcaagttc
    23341 aacaatccga tccaaatctt ctaatcttgc ttgtctgatt ttcattgttc ctccagataa
    23401 aagggattaa accaaatcat actatagccc tggctagttg catagagcaa agtttcttct
    23461 tcatcaacaa aaccgttcat ttcaaaatag gaaagcagct catcaggact ctccaaacga
    23521 atccctttgt aatccagctc aactgccacc tctttcaagg ctgcaagaag aagtgttccc
    23581 aggccctgtc tctgatggtc agactcgatg actaaagaat gtacttttag acattgcgga
    23641 ttgtctgact ggggacttga taaaatatag cctaaaagtt gattttcatc cctagctaga
    23701 agaaaggtat ccgcacactt acggatactt tcttctaaaa tatgggaaag ttgctgcttt
    23761 tcagctggaa aagacgaggt ctgaagtgcc cctatctcag gcaaatcaaa cttgcttgcc
    23821 tgaatgatct taattggaat ttccatggga acatcctatt gaacattgct tgtcaagtta
    23881 gacaagagac gctcaaatga gtattcatag gtttggatgt ctcctgctcc cataaagacg
    23941 taaacagcat tgtcatggtc taggagtgga gaaacatttt caacagtaat cacttggtgt
    24001 tttttgttga ttttattggc taggtcttct accttaacgt caccatgatc tacttcacga
    24061 gccgagccat aaatttgcgc tagataaaca gcatctgctt ggtttaaagc atgggcaaag
    24121 tcgtccaaca gggcaatggt tcttgtaaag gtatgcggtt gaaagactgc tacaatttcc
    24181 ttgcttgggt atttctgacg agccgcatcc aaggtcgcaa taatttctgt tggatgatgg
    24241 gcaaagtcat caataatcac tgtatcattg acaattttct cagtgaaacg acgtttaaca
    24301 ccggcaaatg ttttcaagtg ctcacgcacc aagttcaaat caaatcctgc tgtgtaaaga
    24361 agaccaataa cggctgtcgc attcatgata ttgtgacgac caaaggttgg aatgtggaat
    24421 tgccccaagt tttgtccacg gaaatgaacg gtgaaggttg aaccagttgt tgaacgaaga
    24481 agatcactag ctacaaagtc attgccttca gcttcaaaac cataataata aattggtgca
    24541 tcagacgtaa tcttacgcaa ttcagcatct tcaccataga caaaaagacc cttggtaatt
    24601 tgtttggcat agtcgttaaa ggcattgaaa acatcctcga gacttgtgaa ataatctgga
    24661 tggtcaaagt caatgttggt gataatagag tattctgggt ggtaaggcat gaagtgacgc
    24721 tcatattcgt cagattcaaa gacaaaatat ttggcattag ccgaaccacg acctgtccca
    24781 tctccaatca agaagctggt atctgtaatg tgagacaaga catgagacaa catacctgtc
    24841 gttgaagttt ttccatgtgc tcctgctact cccatgctaa caaagtcacg cataaagcta
    24901 cctagaaact catggtaacg tttgtagctg ataccatttt ggtccgcata ggcaatttcg
    24961 acgttgttat ctggacgaaa ggcatttcca gcgataattt ccatatcacc gtctagattc
    25021 ttttcatcaa aaggaagaat ggtaattcct gcctgctcaa gaccgcgttg ggtaaagtag
    25081 tacttttcaa catctgatcc ctgaaccttg tgccccatct ggtgcaacat caaggccaag
    25141 gcactcatcc ctgatccctt aattccgata aaatgatatg tctttgacat gttttctccc
    25201 ctattctgtc attctggtca gattcaactc ttgggcaacc cgacgttctt gttctgtttg
    25261 tttacttttt ttattgtaga tttggctctt ctttagaaaa tcataattgt ttttctttgg
    25321 agcaggtgct gacacttctt cattcttggt agggatagaa tgaacttctt ccgccaagat
    25381 ataatgagac tgggtcaatt tttggctata tttgacaaat tcaccaggat tttccttttg
    25441 gaaaggagtt gtcggttgat tgccctgtct aactagactg ggctgagaat gacgtctcgc
    25501 aaggctgaaa tcctgagtta ggtagttagc agagcgtttc tttttcaagt ccgcacgcgc
    25561 ttcttcacgc gccacctccg catagctctt tccttctttt ttaaccccta aaggagcctt
    25621 tttaggtttt tcgacttgct tttcaatcgg ttttactggt ttttcttcag taataggagc
    25681 ccattctaaa taatttttat ctcgatactc acccttgata ttactgatca gatcagactc
    25741 atcatagaga ttcatgactg gcatttcagt caacatgacc tcgtcatctg acaccaatgg
    25801 aaatcgttct tgtttcattt tctatttcct ttcaacactt cattatagcg tattgtcttg
    25861 atttttcaag tgctggcttc agaaattccc aaaatttctc taatttctgc tagggtcaga
    25921 ctaccacgtg actctgtgcc atccaatact tgtgacacca gatgtttctt ttgttcttgg
    25981 agttcctgaa ttttttcttc aatggttccc ttggtcacca agcgatagac ctcaaccgtt
    26041 tcttcctgac ccatccgatg ggcacggcca atggcttgcg cttccaccgc aggattccac
    26101 caaaggtcaa ccaagatcac tgtatctgca cctgtcaggt tcagaccgac cccaccagcc
    26161 ttgagggaaa tcagaaaggc atctctttct ccttggttaa aggccttggt catgtcttgt
    26221 ctttccttgg ctggggttga acccgtaatt ttaaaggatg tcaggcccaa gtctggcagt
    26281 tcttgttcaa ttttttccaa cattcccttg aactgagaga aaatcaagac acggtgtccg
    26341 ccgtctgcca cctgtaccag taggtctcgg agactatcta gtttgccgct ggctccctga
    26401 taatcttcca taaacagggc aggagtgtca catatttgac gcaagcgcat caaaccagat
    26461 aaaatttcca cacgacttcg ctgaaattcc tgttctgaca cttgagccag atggtctcgc
    26521 atctgttgta actgggcaag gtaaatagcc ttttgctggt cttccagttc atttttataa
    26581 accacctcaa tcaagtctgg caattcagtc agaacttctt ctttcttgcg tcgcatcacg
    26641 aaaggcttga taaactgagc cactcgctct gctggcaatt tcataaattc tttcttgctt
    26701 ggcaaaagtc cgggcatgac gatttggaaa atagaccaca actcacccag atggttttca
    26761 atcggagttc ctgacaaggc aaagaccgac ggcaccacaa attgtctcaa ggtctgggca
    26821 atcttggtct gggcattttt catgacctga gcctcatcta agaaaaggaa gtcaaaggcc
    26881 atcccctgat aaaactcact gtcctgacgg aaggtggcat agctagtcac atagatttga
    26941 tggctctcgg caagaatctc ctcacgactt gctttcaaac catgaacaac agccacatcc
    27001 aactgtgggg caaatttctg aaactcatct gcccagttgt aaatcaaacc tgacggagcg
    27061 agaatcaaaa cccgactttc ttttgtcact tgactagtca aaaaagcaat ggtctgaagg
    27121 gttttcccaa gtcccatatc atcagccaaa atcccaccaa aaccataatg atggagcatc
    27181 tgcaaccagc caattccctt ttcctgataa tctcgcaagt cagcctgaac ctgagttgcc
    27241 tgtcgaggaa agtcctctgg atgtgtcaaa tcctgggcca gattctggaa ttcttgtgaa
    27301 aaagaaacac ggtctcgccc ttcaaagaga tgagctaaac tgtaggccaa ggatttccga
    27361 gcctgcaagg tcccatcttt taattcaaat tgccccagtt cctgtagatt ttggcgaatt
    27421 ttcttggttt cttcatcgaa aaagtaaact tgattagacg aatcaatata aaaatcctga
    27481 ttggcaacca aggcctgcat ggcttggtcg atttcctcct gggcaatatc ttgaaaatca
    27541 aactggattt ccaagagacc tcccttggag gcaatctgca cctgaggact tgctaggcta
    27601 taaagctctt ctagtttatc tgataggtca acatgcccga gtttttcaaa gactggaatg
    27661 atatcatgaa aaaaatgata gacagactcc gcttttaagg cctgacgcca agattgaaaa
    27721 tcggcttcaa agcccgcagc caaacagact tggaaaattc tttcttctaa gtctgcgtca
    27781 cttgaaaatg gtaattcttc tagctcttgt cggctagata cctgtctatt tccataatca
    27841 aactgaattt ccaaacgaat ccgattatct tcttccctgt caaagtaaaa agagggcgca
    27901 aaagttttga tttgtagacg ttctggagct gaaacggtgc ccatctggat aaaaagagtc
    27961 agacaggagg ccaatttgtc tcgatcactg ctatcaaatt gcaggtattt ctttccttgt
    28021 tgacccacag gtaacgcttt aatttccttg agaagacgca tctgctggtc tgttaaaaaa
    28081 taaacctgac ctttatggaa aagtactgct ccctgataaa agacattgac cctaggactc
    28141 tcactgattt ccatttcaaa atagtccgag tattctgtta ctgtaaaggc aaatagattg
    28201 gcatcagcat gcatatcctg aaaaagcagg gtttggtagc tatccacttg atggtcaaat
    28261 tgaaaatggg gcaaggccat cagtaaattc acaccctgct caaaaaaggt cagagggaaa
    28321 aagaggtgcc gaccttggtt ttggaaaaag aggtctgaaa ccagcccttc ctccgttagt
    28381 ccgtgcaaga aagtcaaaag ttcttggctg gcatcatcaa aggcttccca agaaagagac
    28441 tcctcataaa tcttgccaat catatacgac tttctctgct cgacaatcct taaaaaaagt
    28501 ggaatatccc gaatgacata gtatttttgg ctattgattt ggccgattct cagagtccac
    28561 aagatatgat tggttcctgc ttccacctga cccacagctg ataactcata ggcgcattct
    28621 gattttggag ataaaattcg atccaaaaac ttgccaccca aggtcacctt ggtttcaacg
    28681 gcctcttttt cttcatgacc ttcttccaga ctccacaaga tttcctgacc acgctcatca
    28741 tttttcagaa aatgctctag cgctgccaaa tgcacacagt aacccctctt ttgaaaaaaa
    28801 tcacaggcac aaaaaaccaa atcatcctct aaactatagc gcagttcttc ttctgcaacg
    28861 cgagcgtaga gccgattgtt cttttccttg atgatatcaa ccttaccagt ttcataaagg
    28921 gcaacacctt cgatacgaac tttccccgga atcaatttag ccatattttc acctttacct
    28981 tatcttttta ttataccata ttttcgccta tgaaaatagc cttctaggaa gacttttctc
    29041 ctagaaggct ggatttttaa cgtttggcaa aagtagccac aatccgctga cagacttctt
    29101 gcaacagaga tttgggcata gctatattga tgcgggcatg gagacttcct tcctctccaa
    29161 aatccaaacc acggttgagg ataaccttgg cttcatttct caacaactct tgcaatgttt
    29221 catcagtcag gtcataagct gaaaagtcaa gccaaatcaa gtaggtacct tgcggtttca
    29281 tgaccttgat tttagtctct tttccaaata gatccatcac ataattgatg tggtcttcaa
    29341 agacttgctt gagttcctct agccaatctt taccgtatcg ataggcagct tctgtcgcca
    29401 aataacccaa gcctgaaatt tcatgctgat tattggccaa caggcgtttc tggaaagcca
    29461 gtctcaactt aggattttca atgactgcat aggaattttt tgttccagca atattaaatg
    29521 ttttagtggc actgctcaag acgatagcaa aatttttgaa ggcaggattg atggtattga
    29581 aagactggtg tttgtgacca aagagggtca aatcttggtg aatctcatcc gaaactaaca
    29641 aaacaccgtg tttttggcag agttggccaa tcttctccaa cacttctttt tcccaaacac
    29701 gtccaccagg attgtgaggg ttgcaaagaa catagagttt aacctcctct tccaccaaat
    29761 ccttttcaag ttggtcaaag tcaatctcaa acagactatc cttttccact aaggaattag
    29821 taatcaatct acgattattc aacttgacac tgcgagcaaa gggtgggtag acaggcgtgt
    29881 taattaaaac cgcctcgcct tcttttgtaa aggtttgaat agctgttgag atggctggta
    29941 ccacaccctc gataaagaca agagcctctt tgtcaaagtt gtaaccgtat tgtgtagctt
    30001 cccacttttg aacttcctta attaagtctt cactggcata ggtataacca taaaccagtt
    30061 ggtctgcgta agtttgcacg gcttggcgga tttcaggcaa gaccacaaag tccatatccg
    30121 ctatccaagc tggtagaact tcactatccg tttctgtttc tttccattta taggtatggt
    30181 gccctaaacg gttgggcagg cttgtaaaat catattttcc catctttgtc ttatccttct
    30241 aaggcttggc gcaaatctgc aatcaaatct ctagcatcct caatcccaat agacaaacgc
    30301 aagaggtcat ctgtcaaacc ataagaatgg cgtacttctg ctggaatatc agcatgagtt
    30361 tgagtcgttg gataagtaat aagactttcc actccgccca aactttccgc aaaagagaag
    30421 accttgagac tgttcaaaat atgaggaatg cgtgtttcat cggctacttt aaaggaaatc
    30481 atgcctccac gaccagtgta gagaacttcc ttaactgctg gagaatcctt caaaaaggca
    30541 accacttctt gggcgttagc tgttgagcgc tccatacgaa gagataaggt cttgagacca
    30601 cgaagcaact ggtagctgtc aaatggagac aagactgccc ctgttgtatt gagattgtaa
    30661 aaaagcttct cgtatagttc taaactattg gtcacaacca ctccagccaa gacatcattg
    30721 tggcctgcta gatacttggt tgctgaatgg agaacgatat ctgctccatc ttcaatcgga
    30781 cgttggtaga tagggctata gaaggtattg tccaccacca ctttggcacc cttagcatga
    30841 gccaattttg ctagtttttc gatatcaaat tccaacatca agggattggt tggggtttcg
    30901 atatagagaa catccacatc cttttctaac tcggcaatca actcttcttc tgtattggca
    30961 taggtaaaat ggaaatgacc ttcctgctcc acttggttaa accagcgaaa agaaccaccg
    31021 taaagatcac gcactgccaa gaccttactt cctactggaa agacgctaaa ggccagtaca
    31081 atagctgaca tccctgagct agtcgctagg gcatagtctg ctgactcaat agccgccaag
    31141 acttcctcag ccttactacg agttggattt ttagtgcgcg tatagtcaaa cccagtagat
    31201 cgaccaaact ctggatgctg ataggtcgtt gaaaaatgaa gtggtgtcac caaagcacct
    31261 gttgcctcat ctgacttaat acccgcctgg gccaaaattg tgttaatgtg taattccttg
    31321 ctcatacaat acctccaaat ctatagtaac tattgtacca cttattttca tcaactcatt
    31381 ttcttctttt caagagctag ttatagtttc aaactatata aaaagggagt ttttcctgct
    31441 ccctttaata gactataaaa tggtgaatct caaaagacac cttcactcta tcatttgctc
    31501 ctgcacaaaa cgagcataac gctcatgatt ttccagtagt tccttatgag ttcctgagcc
    31561 agtgattttc ccctcctcta agaagaaaat acaatccaca tcttttaccg tggacaaact
    31621 atgcgctata atcacaaccg tcttctcctt taatacagaa tagaggctac tgataatcgc
    31681 atactcagaa tccgcatcaa gattagcagt ggcttcatca aatataagaa tttcagcatc
    31741 ttttaagtag gttctagcta tttgaagtct ttggctttgc ccccctgaca agaatcgtcc
    31801 gcgttcacca acttcagtat ctagtccctc tttcatggag cgaatctcat cacctagtga
    31861 tactaagtct agcactttca tcaattcatc atcagttact aagcgattca aaccgagaca
    31921 aagattgtca cgaatactgc cagataagac tgcattattt tgtgaaaccc aagcgatttt
    31981 acttctccat tcttttaagt taaaatcata tatacttgat tgctccatta gaatatctcc
    32041 tgaaagcggt ttataaaacc gctctaacaa acgcacaatc gttgattttc ctgatccaga
    32101 tggtccaaca aaagcaattt tttgcccctt gaaaattgaa caagtaatat cctttaagac
    32161 aggtcgattt tcatcataac caaaatagac atggttaaaa ttcaaccctt gtcctgatac
    32221 cgattttcct ccctcaaatt tttctttagg aactgcaagc aagttctcca gtgcaactga
    32281 agatcccttg ctactagaat aaatagttac aaaattagct atattactaa taggattaag
    32341 taattgaaag aggtaaatca aaaacgaaac caaggttccc acagatatat atcctgcgct
    32401 gacccgataa cccccatagg ttagcatcac aactatagtc gcaaagataa ataagagagc
    32461 aaacggggtc tcaaaagaag taaccctatc tgatttcagt gaattgtttt gtaccctttc
    32521 aatacaatta tccaaaacat cctgtacact tttctctgct tggttagtct taattaattc
    32581 atgttcttga atcttttcag tcaattgccc tgttaaaaat ccgatttcct cctgtaaacg
    32641 acgactatac ttttcactga tattggaaag gggcaagata ataaacatca tacaaggaag
    32701 agtgatgaat aaaagtagag aaagattcca atcaagacta aataagacta caatggaacc
    32761 aagtaccata actaaactca gaataatatt tgggaaagtc gtaattaaaa actcacgaat
    32821 gacactcatg tcattgacaa tggcagaagt caactcccca ctttggctct tatcaaagaa
    32881 ggatttctct gcataaatca acccctctat cacttttttc ctgatttttg ctatcttttt
    32941 tcacccgatt gactaaacag atagtaacca atagaagaaa acaaggcttg accaataaaa
    33001 atcaaaaacg attgaaatac tttggaacct atattttcaa tagaactccc atctattaaa
    33061 ccctttaaga taaggggaag caacaaagca agtagactag acagaacaag taagaaactc
    33121 cacataatca ccttagtatc tactcttaat aattttaatt tcataaatac tccttataat
    33181 atttcaacgg ataaagtcgg gaataactca atttgaggat aaaatctaat aaatcttcct
    33241 ataacaaaac gcataacatc taggatttta tatacctgat attatgcgtt tttaagcaca
    33301 aagacttctt acacaaactt atctacaatt agattttatt tgacatgttt tgccaattct
    33361 tcttgggctt ttttattgga ttcttctttt tctttcaacc atttttctct ggcttttgca
    33421 tattcgtctg ttgtgacaat cttatcttgt actttgaggt atttatatga ttcaacccct
    33481 ttattaccag ccaatgaata aggtgctgag aaaggaactg tttttctcaa gctaggcgtt
    33541 ccacctaatg aaacatttgg aatcgctaga gaactatcta tcaaccatgc ttgaatatca
    33601 gcatatttct cataacgttt ggctgggtct tgctccttat tagcctcttc caacatttga
    33661 gtataagtgt caagtccaac tgccttagcc ttgtcattgg cttcaccagg gtcgagtcca
    33721 agattttgta gaaccccacc attcttgaca ctaaagatat caagataggt tgatggatct
    33781 aagtagtcag gactccaacc accattgtaa aggtcgtagt ctttctgaat agcagtctga
    33841 gcaaggtaac ttgagttatc atagtcctct gttgataact gttggatatc aataacgacg
    33901 ttctccgtac ctaaagctgc ttcaatagat tgcttcatgg agcttacttc ttgaatcccc
    33961 ttcttagaag cttggtcaac tgtcgcatcc aaatgaatag ggaattgaac tcccttagct
    34021 tctagctctt tcttggcctc agcaaattta gccttggctt tctcaggatt gtagtaaggg
    34081 tcttgaccat ccgcaaagtt gatgccttgc cattccttac cataattatt gaccatctta
    34141 gaggctacta cttcaccaaa atcttttcct ttgatactta caaaggttgg aggaacaacc
    34201 aagttacgca aaatcttagt cgctccgtct tctccctgag tttgagcccc ataggaggta
    34261 cgatcatagg caaagttaat agcttgacgg aagtttttat taagaactgc ttcctgagtt
    34321 gatttctttt caatgtcggt cgtcttagaa gtaaatttat aagcttgtct atctaggtta
    34381 aaattccagt aataagacgt agcatcctgc atactgtaga tgatatcatc tttgtatttt
    34441 tctttaatcc cttcaaaact tgaactattc ggatagagac gggcatagct ataggctccg
    34501 tctgtaaagt tacgaacaag agcatcttgg tcactaccat catagtaggt caatttcaca
    34561 tcgtctacaa agacattttt agcatcccag tagttaggat ttttcttgta ttcgatgaca
    34621 gattttgaaa caaaagcttt catcaagaaa ggtccactgt acaaaatatt agcagggtct
    34681 accttgccaa aatcatcccc ttttgatttc aagaaatctg cattgactgg gaaaagaatg
    34741 gttgaggttg tttttgagtt ccagtaaggt tctggtcgag ccaaggtgta ttgaacggtt
    34801 tgatcatcaa gagccttgat accgacagtt gaaaagtcgc ttgttttacc agtgatatag
    34861 tcatccaaac ctgcaacaga gtcctgcact agatacaagg cttctgattt tttatcagct
    34921 gcatattgca atcctgtcac aaaatcctgg gcagttacag gtgcatattc ttctccctca
    34981 gaagtaaacc acttggcatc cttacgaagt ttgtaggtat aggtcaaacc atcctgagaa
    35041 acagtccaat cttctgccaa ggatggcaca tagttcccat attggtcatt ttccatgaga
    35101 ccatctacca gattggtcac aatatcattg gttgttgcgc ggttttctgc tagatagttc
    35161 aaactagatg gatcacttga ataaacatag ttataggttt ttgatgcagt gctagaattt
    35221 ccacacgcgc tcaataaaac tcctgtaccc aggacaaaac ctgccaaggt tagatatttg
    35281 ctcttagact ttttcatttc cagaacctcc tgctttaaat atttgtttta ttatacaact
    35341 cttggtttgt ttagtcaatt gttttttcca aaaaatcaaa aaactcattt tctatttttg
    35401 aaagataatt ttagctattt tgacgctaaa aaaggaataa gtttcaagtt ctcctaaaag
    35461 attgttcaac ttattccttt tatttataaa tgctacctta cgaaagctta aattttgctc
    35521 gcaggcgatc tgcttgggct tttcctatta ccttatctaa taaataggta cgcagactca
    35581 taaccatata aagtccaccc cccatggcac cgacaagagc tacataaaag aagctccaca
    35641 aacgtccact tggttggaag aaaaatccta acagccactg gatggttcct attaacagaa
    35701 acatgactag ggtcagcaaa ctgattaaaa tggttcgctt caaaatcacc ttgcgcttga
    35761 caccagttac tttacaaata tcccgataca tcaagacgtt aggaatgatg agagcaatgg
    35821 ttgttgaaat caaaggacca taactgtgga agagggcgat ggtaggtagt tgcaagacta
    35881 gcttggcaat agaaccatag ataaaataga gaacggcctt gcggttgcgg aacatggcct
    35941 gaagcattgg agacaagacc atgtacaagc ctaaaataat agactgcaaa actgcaaaga
    36001 caaataagcc cagagccaaa ctatctggct taccatagaa gaccgtataa agaggttctc
    36061 ctaccataac cactccaacc gttgctggta gcaagaacat aaagagtagg gtgagactgt
    36121 cctgaacgag acgagaagcc gctttcaagt cccccttgac atagttttcc gtcaaaagtg
    36181 gcaaaccaac actcccaatc gaaaccccta cagaaatcaa aatcatcgtg attttattag
    36241 gattggctga gaaataagaa aacatgacaa ccaagtcctc attgctgtag ttggtaaacc
    36301 agctcatact attgataaag gtcagctgat ccaaaatctg gaagagctgg atggcagacc
    36361 ctgtcaggat aaaaggaatg gcttccttaa tggtatcaac caagagacgc ttactgttaa
    36421 tcttatctcc tgtttcaaag actcttttga gtgaaccttc ttgggcaagg aaataaatca
    36481 agactgcaaa actggctacc ataccgacaa aggcagcaaa ggttgattgg gtaacggctg
    36541 ctagataatc tcctgaaccg agcttcataa tgataaaggt tgctaggagc atccagataa
    36601 cacgaatgac ctgctcagca atttggctca tggcataagg tttgaggtta ttcatccctt
    36661 ggaaaaatcc tcggataaca ctcatagacg ggaaaatcaa gactccccaa gccaagcttt
    36721 gcatgattgg gatcaagtct ttgcccacgc cagacaagtc tgctagccaa ggagcaaaga
    36781 catacaagac taaagcaaaa accaggccta gtcctgtcat aaagcctaag aagctccgaa
    36841 tcagggcaaa gctatgctct tcttctcgca tggtattata cttggcaact tgcttggcca
    36901 ccgcaactgg aatccccgct gttgaaacca acaagaacca agcatagata ttgtaaccca
    36961 tggtaaagag accatttgcc ttagctgcat aagcccccat ccagatgtac caagggataa
    37021 tgtaaacagc cccgagtagg cgactgataa agttactagc cgttagccaa gcagtccccc
    37081 gtaacatctg ggcctgctgg tgattgtttt cgtgcgacat agattcctca ttcaattttg
    37141 ataactagga aaaactcctt atcttccatt ataaactttt ccttgttact tgtaaatttg
    37201 cgactttcgg tttacaatag aaagtatgat aaaaattgaa actgtattag atattttaaa
    37261 gaaagatggc ctttttcgcg aaattattga ccaaggtcat taccactaca actacagcaa
    37321 agttgttttt gatagcatca gctacgacag ccgaaaagta acagaagaca ctcttttttt
    37381 tgcaaaaggt gctgccttta aaaaagaata ccttctttct gctataacac aaggtttagc
    37441 ttggtatgta gctgaaaagg actacgaagt cggtatccct gtcatcattg tgaacgatat
    37501 aaagaaagcc atgagtttga ttgccatgga gttctatggt aatccacaag agaaactcaa
    37561 aattctcgcc ttcacaggaa ctaagggtaa gacaacagca gcttactttg cttacaacat
    37621 cctatctcaa cgttacccaa ccgccctctt atcaaccatg aatacaactt tggatggcac
    37681 aactttcttt aaatcttcat tctcaacacc tgaaaatatc gacctctttg acatgatggc
    37741 tcaagctgtt aagaatggaa gaagccatct cgtaatggaa gtctctagcc aagcctatct
    37801 agtccatcga gtctatggac tgacctttga tgtaggagtc tttcttaaca tcactcctga
    37861 ccatatcggc ccgattgaac accctagctt tgaagactat ttctaccaca agcgtctctt
    37921 gatggaaaat agccgagcag tcatcattaa cagtgacatg gaccacttct cagtcttgaa
    37981 agaacaggtt gaagatcaag accatgattt ctatggtagc caatttgata accaaatcga
    38041 gaattccaaa gcctttagct tttcagctac gggtaaactc gctggagatt atgatatcca
    38101 actcattggc aacttcaacc aagaaaatgc agttgctgct ggacttgctt gtctccgtct
    38161 cggagcaagt cttgaggaca tcaaaaaagg catcgctgca acccgcgttc ctggtcgtat
    38221 ggaagtcctc actcagaaaa atggagccaa ggtcttcatc gactatgccc acaatgggga
    38281 tagtctgaaa aaactcatca atgtggttga aactcatcaa accggaaaga ttgctctggt
    38341 tctgggatca acaggaaaca agggagaaag tcgtcgtaag gactttggcc tcctcctcaa
    38401 tcaacaccct gagattcaag tctttctgac tgctgatgac cctaactatg aagacccaat
    38461 ggccattgca gatgaaatta gtagctacat caatcatcct gttgaaaaga ttgcggatcg
    38521 ccaagaagcc atcaaggcgg caatggctat cacaaatcac gaattagatg cagttattat
    38581 tgcgggtaag ggagccgatt gttaccaaat catccagggc aagaaagaat cctacccagg
    38641 agatacagcc gtcgcagaaa attatttata agaatagaaa aaatcaaggg aaaatgaaac
    38701 ccttgatttt ttcctattat ttagtaaaaa cgtttttcaa accttcaacg gcaccttcta
    38761 cagcaccttt tgcgtcttct acaacgtctt ttgccttagc aactgttttt tcaacagctc
    38821 cttcaagttc tgtcttgctg tctccagtaa ctttaccaaa accttctttg acagcgcctg
    38881 ttgcttgttc caatttgttt tcaagtgaca tatgattgtc tcctttagtt tattccgata
    38941 tgtgttaccg gttacatata gtttataccg aatactaagg aaagtcaaac aatgtgctca
    39001 gaaacaagaa atcacgctaa gtagaaagtt aatctctcct tatcaaaatc gatagccaac
    39061 tcatactttc tatcttcatt ttgcacaata tagcctaaga caaccaaact gtccacaaag
    39121 atatcacggc atttctgcat aagctggtct tttttgagaa atttcaacaa aaaagccgtc
    39181 atatacttga gggcatactc aggattaaca tctcctaaaa tgtcatagag ctcctgctgt
    39241 ttttctgtca aaggatactg ctgtttgacc ttgtaaaaat aattggacag ggtcatttta
    39301 atgcgcgcaa agtccgtctt ttcaactaaa atagctgcat tgatttgatt gcgcaattcc
    39361 gtctcaaaac tctgctccaa caaggcttga tagaccggac tagcttctct gacaaaaatc
    39421 tcttgatcca gttcaagact atcaagtgat tcaagcgtag gaaaattaag gtaatagcgc
    39481 ttattttcac gtagaatcaa gcttgccttt atatactctt ccatgagttt atcaactgca
    39541 acatctggaa actgagactt aatttcccgt agaatcacat cctcatactg gtccagatag
    39601 cggatcaatt ctccaaaaat ggctgtctcg tcaaacgaga tggattaaaa atctgaatca
    39661 tgaagatccc tttctttaca ttgtaacaga ggcgatgctg ccaactgact aggattggtc
    39721 ccagcttggt tcagaggaac aggcagacct agttctatgt aatactccct ccaaaaatca
    39781 ctaatctcct gttccccatc tccaaatttc ataggaatag tttcaaaaat aggtaggatt
    39841 tctgctatat actgggagca gtagaaagca gctgcatctg gatagaaaga agcattgtag
    39901 ggtgctccaa gatgtctgca agctctttcc ttcaccgact ggatatccat ttctgggtaa
    39961 acatagaggt cgtataaatg attggactca aagaagtctg ccggttcttg acagacaaca
    40021 ccagcctgtc cactagcatg ataaatcatc ccatccaaat aaatggcaac atggctatag
    40081 ttacctgtgg aagtctggat ggcctgtccc atgtctgacc catctctcac aaaaatcaaa
    40141 tcgccatttt ctaacatact tcactcctag aaaaaaagga gccgaaactc ctttcgatat
    40201 aaggcaaact agactttccg attttgagct gacacccaaa atcttacgca ttaaagcttt
    40261 cagttaattg aggtaccact tgtttcttac gtgaaacggc accagcaagg aaggcatgat
    40321 tgttttcaag tttgaagttg aaagccgctt cgaccttgtc catgttggca ccaagagcca
    40381 agatttctga gtttgagttg acgatatctg taatcatcaa gacaaagtca gagtagccgt
    40441 ttgattcgtt ggcagcttgc attgcagctt caatttctgc ttggcgttcc aaaacttcag
    40501 cgatgtcaac tgtgttcact tgggcaacac ggacattatt tccgttgagt tcaaaagtct
    40561 tagcatcgat gtcaatcaat tcttcagcag atttgctagc caagttggta ccagctttca
    40621 acattgccaa accatattct tccaagttca caccagccaa ttcagccaat tcaggagcaa
    40681 tgattttatc tgttgggtgt gttgttggtg atttcaaaag aagggtatct gaaatcaaac
    40741 ctgaaagcat caaacctgca atctctttag gcacagctac accatgttct ttgaacatac
    40801 ggtaaacgat tgaagacgct gatccaactg gctccaaacg catgtaaagt gggcttgcag
    40861 tttcaaagtt agccacacgg tggtggtcta caacaccgta aacttctact tcagcgatat
    40921 ctgatacaga ttgttggaat tcattgtggt cagtcaagat aacttgctct gccccctctg
    40981 ctttggcaga agtgataaca cgtggtgctt ccacaccaaa atagttcaag acaaaggctg
    41041 tttcttcatt tggagttcca agggcaacag cttccgtatc caaaccgtaa gcttcttttg
    41101 caaggtaggc aaaagctaca gatgatccga tggcatctga gtctggattt tggtgaccaa
    41161 atactagaat cttggacatg ataatacctc attttgttta ttttctttat tgtagcattt
    41221 ttttcgcttt ttgacaaagg taagaggagt caaaggactc cccacgattc ttcaaaataa
    41281 ctgagtaaat ttctatcttg attttcagat aaaaattctt ccttctgtgg cctcttctta
    41341 cgcttgagaa gagcttcagc cgacatggct tcttccttac tggcaaaacc ttgagcatag
    41401 ataagtttga ctggcaagcg tgctcgtgta tatttggctc ccttcccact attgtggata
    41461 gcgaggcgtc ttctcacatc agtcgtatag cctatatagt aggatccatc acgacactcc
    41521 agaacgtaca tataagcctt atgatccata ataaatctct tcgatttcgg gcgtataaga
    41581 gccatcatca ttgtggacaa tcaaaggagg taagacctta aagccacttg ttgagccatc
    41641 cttgatcgcc tcaatcaaaa gcatattggc ttccttttct ctttttggat aaacaaactg
    41701 caggcgctta ggggctagat tatgtcgttt taacgtatcc aaaatatcca gaagtcgatc
    41761 aggacgatga accatggcca aacgcccatt agacttgaga atactctggg cactatgaca
    41821 gatttcttcc aaattagtcg tgatttcgtg tcgagccaag agataatgtt cactctcgtt
    41881 cagattagaa tgaggatcca ccttgaaata gggtggatta cacaaaatca tatccacctt
    41941 actcccctga atgtgagcag gcatattttt caaatcatcg cagatgacct gcatttgctc
    42001 ctctaatcca ttcaaacgga cagagcgttc agccacatcc gccaaacgct cctgaatctc
    42061 aacagacaat atctgtgctt gagtacgagt gctagcaaaa agccccactg ctccattccc
    42121 agcacagaaa tctacaatca accccttctt aggaaaacgt ggaaatcgtg ataagagaac
    42181 actatccacc gaatagctaa aaacctctct attttgaatg attttgatat ctgtcgaaaa
    42241 gagctggtta atgcgctctc ctgattttaa taattgttct tcttccatgg tcctattata
    42301 gcaaatttat attaacatta caaaaaatat aaaactctaa actacttctt cttttttaaa
    42361 tagtgcaggg cttctccagt ccagattggt agcattcgtc gaaagggagc aaaaccgtag
    42421 ttaaagcggt cgcttgaaaa gcgtctccgt ctaggaaact ggtacttttc ttcctccaaa
    42481 gtgcggatag aaagactggc tttccccgta aattcatcta aatccactac ctgaacttga
    42541 acctcttcat cgactttcaa ggtttcatga atattttcaa taaatcctgt ccgaatttct
    42601 gaaatgtgaa tcagccccgt atcacccgtc tctaactcaa caaaggcacc gtagggctga
    42661 atccctgtaa tacgcccctt tagcttatca ccgattttca tcttagtcct cgatttcaat
    42721 agtttcaatt acaacatctt caactggctt gtccatagct cctgtctcaa cagcagcaat
    42781 ggcatccaag acagcgtaag atgcttcatc agctaactga ccaaaaaccg tgtgacggcg
    42841 gtctaggtga ggtgtcccac cttgattggc atagatttct gcaatcggtt ctggccaacc
    42901 accacgagta atttctttct tagaataagg taggtgttgg ttttgcacga taaagaactg
    42961 gctgccgttg gtatttggac cagcatttgc catggaaaga gcaccacgga tattgtaaag
    43021 ctcttctgag aattcatcct caaaagattc gccgtagatt gactcgccac ccataccagt
    43081 tccagttggg tctccacctt ggatcataaa gtccttgata atacggtgga aaatgacacc
    43141 atcatagtag ccatcttttg aaagagatac aaagttagcc actgttttag gagcatgttc
    43201 agggaaaagc ttgatacgta agtctccgtg attggtctta atagtcgcaa gaggaccttc
    43261 tactgtttca atgtctactt gtggaaaatg caattctttt tctaccatac caaatacttc
    43321 taaggcatca aaaatgccat cttcttctaa tgtttttgta atataatctg ctttttcttt
    43381 gattttatca tgagaaattc ccatggcaac gctgattcca gcataatcaa agagttccaa
    43441 gtcgttgaga ccatctccaa aaaccatgac cttctctggt ttcaagccaa ggtgttccac
    43501 aaccttttcc acccccgtcg ctttggagcc tgaaatcggc acaatatcag aggaatgttg
    43561 atgccaacga accatgcgaa gtttgtctga gagactgtca ggcaagtgca agtcatctcc
    43621 cttatcttca aaagtccaca tctgatagat atcttctttt tcatggaaat cgggatctac
    43681 atctaagtcg ggataaattg gattgatagc ttcactcatc atatcggtgc gagtcgacaa
    43741 cttggcatca tgactcccaa ccaagccata ctcaattcct tcttgcttag cccaagagat
    43801 atactcctca acatctgact ttccaatctg atgctgataa atgacctgac cttttttatc
    43861 ttcgatataa gccccattca aagttacaaa aaagtcaggc ttgagatcac gaatctctgg
    43921 aacaacacca aaaatgccac gtccagaggc gattcctgtt aaaattcctt tttcacgcaa
    43981 ctgtttaaaa acagtgggaa ttgtagttgg aataaaccct gtctttgaat tccgcaatgt
    44041 atcatcaata tcaaaaaaga caatcttgat cttctttgcc ttgtatctta atttcgcgtc
    44101 catctcacta cctctttcaa tctaactctt tccattatat cataaagtag gcaaatcccc
    44161 tattttcaaa aagtttatca tttttatttt aatttcttgg atgagaaaag agacatattt
    44221 atgaaaaagc tccatcgtgc ttgtaatgtg ttctcttgtt ttcaaactcg taaaaaggga
    44281 gccactgagc ctaactcgct ctctcatttc aaagcttgtg aaaaaagacc cgttggggtc
    44341 ttaattcgct ttcttgtttt caagctcatg aaaaagagac ccaaccgggt cttttcttta
    44401 atcttcgttt acgaaaggca tcaaagccat tacgcgagcg cgtttgatag ctgttgttac
    44461 tttacgttgg tttttagctg aagttcctgt tacacgacga ggaaggattt tcccacgttc
    44521 tgaaacgaaa cggctaagaa gctcagtatc tttgtaatca acatattcaa ttttatttgc
    44581 tgcgatgtaa tcaacttttt tacggcgttt gaatccgcca cgacgttgtt gagccatgtt
    44641 ttttctcctt tataagttta gttgtccatt agaatggtaa atcatcatct gaaatatcca
    44701 atgggtttgt tgctccaaat ggattttcat tacgtgaaaa gtctggtact gaatttgtag
    44761 gtgctgaata gtttgcagtt ggtgcagagt aagctccacc tgtgtgaccc tcacgcacac
    44821 tacggctttc caacatttgg aaattctcag ccacgacctc tgtcacgtag acacgttgtc
    44881 cttgctggtt atcgtaacta cgagtctgga tacgacctgt caccccgata agtgagcctt
    44941 ttttagccca gttagcaaga ttttcagcct gttggcgcca cataacgaca ttgataaaat
    45001 cagcctcacg ttcaccattt tgactcttaa atgtacggtt tactgcaaga gtaaaagtcg
    45061 caactgctac atttgatggg gtataacgca actcagcgtc acgtgtcata cgccctacaa
    45121 gtacaacatt gttaatcata gtttaccttc ttacgcgtca attttgacga tcatgtgacg
    45181 aagaatgtca gcgttgattt ttgaaagacg gtcaaactct ttaagagctg catcgtcatt
    45241 tgcttcaacg ttaacgatgt ggtaaagtcc ttcacggaaa tcttggattt cgtatgcaag
    45301 acgacgtttt tcccaagttt ttgattcaac aacagttgca ccgttgtcag tcaaaataga
    45361 gtcaaaacgt gctaccaaag cgtttttagc ttcttcttca atgtttggac gaatgatata
    45421 aagaatttcg tatttagcca ttgatatgtt cctccttttg gtctaatgac cccaagactt
    45481 tgcaaggggt aagtgaggtt cgctcacaat aaactattat actagaaaaa atttttttac
    45541 gcaagtaaaa acactagaat tcgaaaaaac gccacatggg cgttttcctg ttcttatggt
    45601 ttgatacggt gcaacatacg tgggaatgga atagcttcac ggatatgttt tgttcctgct
    45661 gcgaaggtta ccatacgttc gataccgata ccaaatcctc cgtgtggaac tgtaccgtat
    45721 ttacgaaggt caaggtagaa ttcatattct gtacgatcca tgccaagttc atccatctta
    45781 gcgacaaggg catcgtaatc ttcctcacgc atagacccac cgataatttc tccatagcct
    45841 tctggagcaa gcaagtctgc acaaagcacg cgctctggat ttccaggaac tggtttcatg
    45901 tagaaggcct tgatggctgc tggatagttc atgacaaatg ttggcacacc aaagcggttt
    45961 gaaatccaag tttcgtgtgg tgacccaaag tcatcaccat gctcaagatg ctcgtagtca
    46021 gcatcttcat cattttcatg ctcttgcaag aggtcaatgg cttgatcgta agtgatacgt
    46081 ttgaatggct ctgcaatgta gcgtttcaag agttctgtat cacgttccaa ggtttccaag
    46141 gcttgaggcg cgcggtcaag aacaccttgt agaagagctt tcacataagc ttcttgcaag
    46201 tcaagcgact catcatgtgt caagtatgag tactcagcat ccatcatcca gaactcagtc
    46261 aagtgacggc gtgtttttga tttttcagca cggaaaactg gaccaaagtc aaagacacga
    46321 ccaagagcca tagcccctgc ttctaggtaa agttgacctg attggctcaa gtaggctggc
    46381 gttccgaagt agtcagtttc aaagagttct gtagaatctt ctgccgcatt tcctgaaaga
    46441 attgggctgt caaacttcat aaaaccgttc ttgtcaaaga actcataagt tgcatagata
    46501 atagcgttac ggatttgcaa cacagctact tgcttacgag agcgtagcca caagtgacgg
    46561 ttatccatca aaaagtctgt tccgtgttct tttggtgtga ttgggtagtc ttgagattca
    46621 ccgatcactt cgatgtctgt gatgtccaac tcatagccaa atttagaacg ttcgtcctct
    46681 ttgacaatac ctgtcacata aacagacgtt tcttggctca agcgtttgat aacatcaaac
    46741 ttctcaagtc ccacttcttc accaaatttt tcgacaaagt ttggtttaaa agccacacct
    46801 tgaaagaagg ctgttccatc acgcaattgt aagaaagcga ttttcccttt tcctgattta
    46861 ttggcaaccc aagcgccaat cgtcacttcc tgaccaacat agtcttttac gtcaataatc
    46921 gttacacgtt ttgtcattat ttttcctttt cttttttatt ctttatggca aaccacctct
    46981 atattgttcc catccaggtc aatcataaaa gcagcatagt aaatcggacg ctcacttcga
    47041 taaccaggag ccccattgtc tcgcccacct gcctctaagc cagcctcata acaagcctaa
    47101 acttcttcct tattttctgc taaaaaagca aaatgaacag gatcttgtgt tccctgagtc
    47161 agccaaaaat caccaccagg atgagggctg ttcggggata gaaaactaat tagagaacta
    47221 gtcttaaaag ccaatttata gtccaaagga gcgagaaaac tcctataaaa tccttctgaa
    47281 atttgtaaat cctttacctt aatctcaaaa tgatcaatca ttctcactac ccataaatgc
    47341 tttcaagcgt tcgactgctt ctttaagcgt gtctaggtct gtcgcatagc tgaggcggac
    47401 attttctggt gctccaaatc cagctcctgt taccaaggcc acttcggatt cttctaagat
    47461 agcagttgta aagtctgtca catccgtgta gcctttcatc tccatggcct ttttgacatt
    47521 tgggaagaga tagaaggccc cttgcggttt gaccacttca aatcctggca cctctgcaag
    47581 gaggggatag atggtattaa gacgttcctc aaaggcctga cgcatgcttt ctacagtatc
    47641 ttgctcacct gatagagcct caactgctgc atattgggct actgctgacg gattcgaagt
    47701 tgtttgacct gcaatcttgg acatggcagc gataatgtct gcttctccaa cggcataacc
    47761 aatccgccaa ccagtcatgg cataggtttt agacacacca ttgatgacca ctgtttgctt
    47821 gcgaatcgct tccgataggc tagaaatcgg tgtgaactca tgaccattat aaaccaagcg
    47881 gccatagata tcgtctgcta ggatgagaat atcattttct acagcccagt ttccaattgc
    47941 caagagttcc tcacgggtgt aaatcatacc tgtgggatta gatggcgaat tcagcaccaa
    48001 aaccttggtc ttgtcagtgc gagctacttc taactgctct acggtcacct taaagtgatt
    48061 gtcttcctta gcagaaacaa agacgggaac gccttctgcc atcttgacct gatctccata
    48121 gctaacccag tatggggttg ggatgatgac ttcatcacct ggattgacca cagccataaa
    48181 gaaggtatag agagaatatt tggctcccgc agcgactgtc acttgatttg acgctacaga
    48241 atagccgtaa aagcgctcaa agtagctatt gaccaccgcc ttaagctctg gcagacctga
    48301 ggttactgta taaaaagaag cacgcccatc tcgaatcgat gcaatggcgg catcttggat
    48361 atttttggga gtagtgaaat ctggctcacc caaggttaga gacaaaatat ctctaccctc
    48421 agccttcagt gctttggcac gggctccagc agccaaagtc acactttctt ccatttctaa
    48481 aacacggttg gataatttca taggccctcc ttgttgacca atgctcctgt ttcaaaatct
    48541 actagataaa aatcagatcc tgacttgact tcccagattg gcttatcttg ataacgacca
    48601 aagataatct tgtcaatttc gccagctccc ttttccttag aaaccgtttc tgctttttct
    48661 tgtgaaacac cctgatttag ctgataaacg taaatcttat gatcatcttt accaatcagg
    48721 acagcaagcg cttcttgctg tttattacga ccaagaacgc tgtaataaga ttccaagcca
    48781 ttgtataaat caacctgatc gacctgctct aatcctgcat actgctgagc taatttttct
    48841 ccttcacttt tagctgtttg atagggtttc atgctaagag aaaccatata cagaaaggaa
    48901 gcactgataa ccacaaacaa aatcgtcatc cctagaccat actgccacag tagattattt
    48961 tttgctttgt tttgtctttt tttcactcgt ctattttacc atctattaag ctttattaca
    49021 agtgaatata agaatactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc
    49081 tcaaagctgt gctttgagca accaattcta tttctccctt caaacaaaac cgatttttaa
    49141 agtgaaacag ttcttacttt ttcagtcaca aatgattaga gtttccgaaa ttttacaatt
    49201 ttcaaataaa ctctgagcaa atttcttgca agttcttttg ttttgttgta atatatttta
    49261 taacaacgag agagttctcg aaattttaag aaaaaggaga cacatcatgt ctaaaaaagt
    49321 attatttatc gtcggatcac tacgtcaagg ttctttcaac caccaaatgg cgctcgaagc
    49381 tgagaaagca cttgctagta aagcggaagt tagctacctt gattattcag cccttcctct
    49441 cttcagccaa gatttggaag tcccaacaca tccagctgta gctgccgctc gtgaagcagt
    49501 tctcgttgcg gatgctatct ggattttctc tccagtctac aacttctcta tccctggtac
    49561 agtgaaaaac ttgcttgact ggctatctcg tgcccttgac ttgtctgata cacgtggcgt
    49621 ttctgccctt caagacaagt ttgtcacagt atcatctgta gccaatgcag ggcacgatca
    49681 acttttcgct atctacaaag acctcttgcc atttatccgt acacaaggcg ttggtgattt
    49741 cactgctgca cgtgttaatg actctgcctg ggcagacgga aaattggttc ttgaagaaac
    49801 agtcctaaac tcacttgaaa aacaagctca agacttggtc gaagcgattc agtaaaaaat
    49861 agaaaatttt agaaagtcta aatcaattat agccacctaa tagggtggct ttttatttct
    49921 caatcaagat tttcagacaa acatcagcca tccaaaaaaa ccttgagcgt taccactcaa
    49981 ggtttttagc agtcttttcc atcataggaa aatcattcta agaatattct ttcattcgag
    50041 atctatcact tatcatcttc tttcgagaaa ttagtgactg cttttggctg attaatcttc
    50101 ttttcttcgt cctacatata ggctcaatag aataccgcta cctaaaagaa cagagatata
    50161 atcagattgg cttccagttt caggtaattt gtccccacgg ttttctttac ctgtagttgg
    50221 tgctactggg ttagcattta ctggagttgc tggttgagcc ggtgttggta ctactggttg
    50281 ttctggaacc gctggtactg gtttaaccgg tacttctggt gtcggcacat ttggtgttgg
    50341 aggaactggt ttgtcatttg atggtgttac tggtttgtct tggcatccac attttccatc
    50401 tttaccgtct ttgccgtctc gaccatcacg tccatctcta ccatctttgc catctttaat
    50461 aactgttttt gtcacagttc catcagaatt gatgattgtg attgtatgac taccatcacc
    50521 attatcttca acagaaactt ttggtgattt accatcttta ccgtctttaa tcaccatttc
    50581 tgtcttactt ccatctggat tagtgattgt aacaacgtga gttccatcgt tattattttt
    50641 aacagttaca gttggtgaaa cgctatcttt gccgtcttta ccatctttga taatagattt
    50701 gtattcacga ccatcagaat ctacgattgt taatgtatga gttccatctc cgttatcaac
    50761 gatgttcgct tttggtgatt taccatcaaa gattgtagtt gtcgttcctt taccatcagc
    50821 acctgtcact ttaatagtgt gtgaaccatc gttgttatca ataacttcaa gttttggttc
    50881 tctaccatca cgaactgtag ttgttgtagt tgttccgtct gagttcacaa ctgtgatagt
    50941 gtgagttcca tctccattat ccacaacctt agcagttggt gatttaccgt cacgaactgt
    51001 ggtttcagaa acagtaccat taccgttttc cacacgaata gtgtaagtac catcttggtt
    51061 atctctaact gttgctactg gtgatttacc atctgtaatg attgtttcag tagttgttcc
    51121 gtcaccattt agaacagaga tcttatgagt tccattttgt tcatcagtta tagtcacttt
    51181 tggtgattta ccatcacgaa ctgtggtttc agttgtcaca ccttctggat ttgtaataac
    51241 gattgtatga cttccgtctc cattatcacg aacagatgcg gttggagtac gtccgtcaac
    51301 accgtcacgt ccattcttaa caacaaactc attcttagtt ccgtctgggt tagtgattgt
    51361 tactgtgtgg cttccatctg gattttcagt agttgttgct gttgcagctt taccatcttt
    51421 accatctttg atgatagcgt tggtaacatt gccgtctcca tccgtcactt taactgtgtg
    51481 tgttccatcg ttgttatccg ttacttcaac tttaggagtc ttaccgtctt taccgttttt
    51541 aacaacagtt tctttagttg aaccatctgg atttgtcacc gtaattgtgt ggcttccatc
    51601 tgggttttct gttgtagtga tattcgcagt tttaccgtct ttaccatctt taacaacagt
    51661 tgttgattca gaaccatcag gattttgaac tgtgatagtg tgtgttccat ctccattatc
    51721 ttttgtagta atagttggag ctgaaccatc acgtccatct ataccgttat aaatatcttt
    51781 actatttaat actttatcga cacctccgtc gtatacccca ttgtcattaa catcatcata
    51841 aactgtaaca cggataccgt tacgcgttgg agttgcagca gcatctgtat cgcgtctaat
    51901 tcttgaaggt gaagtaatat cttctacttt gattttcggt gcacggccat ctttaccatt
    51961 cttaccatct tttacagtga aggttactgg atctttacct gatgttgtga tagttacaac
    52021 agtgctttgg ccatcttgtc cacgttgagt agtgattgta ggagtttgtc catcctgacc
    52081 ggttgcacca gtttctccct tagcaccatc ttgaacaaag actgtagttg gttgtgaacc
    52141 atcatggtta tcaacagtga tttctacacc tttatgccct gcttcatctt tacgaacagc
    52201 tgtaactgta ggtgattttc catctactcc atctctaatt accgcaagtg gtttttctgg
    52261 atttaatggt tgtcctgggt tagctggatc ttcttggtaa actttagttt ctttaccatc
    52321 cttgacagta agcaatgatt taccatctcg tccgtccgcg cctttagcac catctttaat
    52381 catttctgaa cctagaagtt catcagttcc tggtgtatat ttgccgtcat tgttttcatc
    52441 tgtatagaat gtcacaattg tatgagatgg atttccgttt tttccttgga cacgaagtaa
    52501 atcaactttt ggagttttac cgttcagacc attttctccg tccttaacat ttactgtaac
    52561 tggatcttta cctggaacag tgaatgtaat gtcagtacta tggccatctt tacctggagt
    52621 tacagttact tctggagtct taccatcttc tccgcgttca cctttgtcac ccttagggcc
    52681 tttgatgttg ccctctttat cccagttgcc gttattctta acgaagacgt cgcctgtttc
    52741 tgtattaacg tatttatcgc cgtctttacc ttgatgtgct tctgggttag ctttgccgtt
    52801 caacacgtct ttaccgtcac gtccatcacg acctactgcg ccatccgcac ctttagctcc
    52861 ttgcgctcca gttaggccgc gttcgccttg gtctcctttt tcgcctttag caccatctgc
    52921 acctttgtcc cctttagggc ctttgatgtt gccttcttta tcccagtttc cattgttttt
    52981 aacatagaca tcaccggttt cggtattaat atatttatct ccatttttac cttggtttgg
    53041 ttgtggattt acttttccgt ttaatacatc tttaccatca cgacctgctt gtcctgtagc
    53101 tccatcacgg atcgtagttg tcacatctgg acttcctgga ttatgaaccg ttaatgtatg
    53161 tgtgccgttt tgcccctcag ttattgttac tgttggggtt ttcccatcag cacctttttg
    53221 tccatcctct acaaatgttg tttgtggacg acctcctact ggtgtaattg ttactaatac
    53281 accattttta ccattttgaa cacttcgtgt cgctgtaatt gttggtgatt gtccattagt
    53341 accatttctt ccatcagtga tagttgctaa tggttggttt ggattaagtg gttgtcctgg
    53401 tctagccgga tcctctacat agacttttgt ttcagttcca tttttaacag caattagaga
    53461 ttttccatct ttacctggag ttcctggatt accttgttct ccttttggtc cttgtggtcc
    53521 cgctggtcct ctttgtccat ctgcgccttt tggtccttga gctccatcac gaccttgaag
    53581 accttgttca cctttggcac catctgcaat aataacttct tttaatttct tatcagaagc
    53641 atctaattca ttatttccat tttgatcata atagaagata acagttgttg tcttatctgc
    53701 ttcatttcgt tgagtcgcaa ctaatggact tgcgccatcg aaaccatctc ttacaacagt
    53761 acttgcaaca ttacctctac cgtcgttaat agtgatagta tgagtgccgt catttttatt
    53821 atctttaacg gttacggttg gagttacacc gtcacgacct gcctgacctt gcgctccagt
    53881 caggccgcgt tcgccttgga ctcctttttc acctttagca ccatctttaa tagcaatttt
    53941 ttgaacaagt tcgtctacgc cctcagtata aacaccgttt ccatcgttat caagatagaa
    54001 agtgaggtta tttgtatcac cgactttatt ttgatcaaca cgtggtttta ctgtatcgat
    54061 tagatcttta gccacaccaa tttgcataat acgtggctta acttctttaa taactgaatt
    54121 actaattggc aatcctagga ttgtttcgtt acccaattga tgaactttat agatgatttg
    54181 acgttgtcct ttttcacctt ctcgaactaa tctagtttga ccttctggta acttactagt
    54241 tggaagatag acagtttgga agtcaatgtc ttctgaaaca gtcttattat tagtaccacc
    54301 atttggattt gcatccaaga ggagtggttt ttctctgaag taatagttag agttactata
    54361 tggatcatta ctagttgcag atcctgattc cccttgacct acattaaaag taacaggtct
    54421 taatcgaaca gacagatcac cattagggtt ggctataggt aaactaaatc taatagtttg
    54481 attactgttt gtttcaacag cagatgttaa actgtagctt gaatgagata caaaatttgc
    54541 accctttctt atatctttag tttgatctct taatctgttt gttgaaatag ttgcacgaga
    54601 tggttctcca aatccattcc ctttgtctaa gagaaagtta ttattaacga actcacctgg
    54661 accagaatat gcaattgaga attctactct atttccaact ctagttgttg ttacatcaaa
    54721 atgaatcggg tttccaccac gtgaattccc attaccacgg aaagtatgag accctctgct
    54781 aatatcagtg aaaacagtat tatttcctag attagttact agtttgttcg ttggagcagg
    54841 tgctgctcta aaaccactat accccttatc caagtcttct ttacggatag ctggattttg
    54901 gcgagcatca gaaaggctca aacctggttt caagatttca ttaatagatg ctggagctga
    54961 agttgtttct actttggcag ctgtgctagt tggcaaaaca gcactatcaa cagctgaacg
    55021 atcagttgtt gcagctggat tagtctcttc cttcgcttca actatattag ctttcttcgc
    55081 tacaggagtt tctgatacag ttcctttgtt agagttagaa cgaagttcta gaccaataat
    55141 agatgattga atagatgact ctgctgctga taattcagct tcagatgcat ctgctttccc
    55201 aagcaagctt tgaccagctt ggatacttgt ttgcaagtta gcagcacttg cttctgtata
    55261 taacttgctt tctaactttt tagcacgcga aagagccgtt aacaattttg acttgtcaat
    55321 agcatcttga tgcgttactt cctctttttt gtcttttgct tcttcaactt tttctgctgg
    55381 tttagtaact tctccagttt taactggagt cgtttctact ggattagcta cagttggagc
    55441 tgcataggtt ttctctttat tagacgcaga aacctctgct agtttagtag ctgcttctcc
    55501 cctaccaaca gaaatactat tggtagtttc ttgtttgttt tcttccgctt gtactgtttg
    55561 gacactagct gctgcaccta ataccaaagc ggtaccgagc aagacactaa ctgcaccaaa
    55621 gtaatactta cgaatagaat aacgttgttg catgctatac cagtcaaatg attttttatg
    55681 tgaatgtttc attttaaact cctag
//
LOCUS       DAVLTM010000004        49804 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLTM010000004 DAVLTM010000000
VERSION     DAVLTM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.004"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            492..788
                     /locus_tag="VYN16_000168"
     CDS             492..788
                     /locus_tag="VYN16_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1402760.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPYNW
                     GAFSNYYHGSQYHWAYVGSNERDNQKTVYAGAHSAAYAFVNTNLGERITFDAGW"
     gene            891..2906
                     /locus_tag="VYN16_000169"
     CDS             891..2906
                     /locus_tag="VYN16_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1402761.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWHDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEDHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            2908..3540
                     /locus_tag="VYN16_000170"
     CDS             2908..3540
                     /locus_tag="VYN16_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402762.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            3681..3959
                     /locus_tag="VYN16_000171"
     CDS             3681..3959
                     /locus_tag="VYN16_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEV1402763.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(4469..4882)
                     /gene="ndk"
                     /locus_tag="VYN16_000172"
     CDS             complement(4469..4882)
                     /gene="ndk"
                     /locus_tag="VYN16_000172"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1402764.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4993..8670)
                     /gene="rpoC"
                     /locus_tag="VYN16_000173"
     CDS             complement(4993..8670)
                     /gene="rpoC"
                     /locus_tag="VYN16_000173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1402765.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPTVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8703..12314)
                     /gene="rpoB"
                     /locus_tag="VYN16_000174"
     CDS             complement(8703..12314)
                     /gene="rpoB"
                     /locus_tag="VYN16_000174"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1402766.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12730..12800
                     /locus_tag="VYN16_000175"
     tRNA            12730..12800
                     /locus_tag="VYN16_000175"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12762..12764,aa:Cys,seq:gca)
     gene            complement(12998..14329)
                     /locus_tag="VYN16_000176"
     CDS             complement(12998..14329)
                     /locus_tag="VYN16_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1402767.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVYQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(14620..15444)
                     /gene="endA"
                     /locus_tag="VYN16_000177"
     CDS             complement(14620..15444)
                     /gene="endA"
                     /locus_tag="VYN16_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1402768.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15483..15671)
                     /locus_tag="VYN16_000178"
     CDS             complement(15483..15671)
                     /locus_tag="VYN16_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1402769.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15664..16947)
                     /gene="murA"
                     /locus_tag="VYN16_000179"
     CDS             complement(15664..16947)
                     /gene="murA"
                     /locus_tag="VYN16_000179"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1402770.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(16971..17201)
                     /locus_tag="VYN16_000180"
     CDS             complement(16971..17201)
                     /locus_tag="VYN16_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1402771.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17259..18296)
                     /locus_tag="VYN16_000181"
     CDS             complement(17259..18296)
                     /locus_tag="VYN16_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1402772.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18280..18768)
                     /gene="coaD"
                     /locus_tag="VYN16_000182"
     CDS             complement(18280..18768)
                     /gene="coaD"
                     /locus_tag="VYN16_000182"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1402773.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(18758..19297)
                     /gene="rsmD"
                     /locus_tag="VYN16_000183"
     CDS             complement(18758..19297)
                     /gene="rsmD"
                     /locus_tag="VYN16_000183"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1402774.1"
                     /translation="MKIISGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19362..20354)
                     /locus_tag="VYN16_000184"
     CDS             complement(19362..20354)
                     /locus_tag="VYN16_000184"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1402775.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20467..21150)
                     /locus_tag="VYN16_000185"
     CDS             complement(20467..21150)
                     /locus_tag="VYN16_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1402776.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21206..21946)
                     /locus_tag="VYN16_000186"
     CDS             complement(21206..21946)
                     /locus_tag="VYN16_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1402777.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            21980..22258
                     /locus_tag="VYN16_000187"
     CDS             21980..22258
                     /locus_tag="VYN16_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1402778.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            22336..23262
                     /gene="yidC"
                     /locus_tag="VYN16_000188"
     CDS             22336..23262
                     /gene="yidC"
                     /locus_tag="VYN16_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1402779.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPLLLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23337..24131)
                     /gene="pflA"
                     /locus_tag="VYN16_000189"
     CDS             complement(23337..24131)
                     /gene="pflA"
                     /locus_tag="VYN16_000189"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1402780.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(24257..25507)
                     /locus_tag="VYN16_000190"
     CDS             complement(24257..25507)
                     /locus_tag="VYN16_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1402781.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25574..26401)
                     /gene="purR"
                     /locus_tag="VYN16_000191"
     CDS             complement(25574..26401)
                     /gene="purR"
                     /locus_tag="VYN16_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1402782.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26668..27609)
                     /locus_tag="VYN16_000192"
     CDS             complement(26668..27609)
                     /locus_tag="VYN16_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1402783.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(27599..28855)
                     /locus_tag="VYN16_000193"
     CDS             complement(27599..28855)
                     /locus_tag="VYN16_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1402784.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(28857..29519)
                     /locus_tag="VYN16_000194"
     CDS             complement(28857..29519)
                     /locus_tag="VYN16_000194"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1402785.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29482..30138)
                     /locus_tag="VYN16_000195"
     CDS             complement(29482..30138)
                     /locus_tag="VYN16_000195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1402786.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRLLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30149..31027)
                     /gene="rsgA"
                     /locus_tag="VYN16_000196"
     CDS             complement(30149..31027)
                     /gene="rsgA"
                     /locus_tag="VYN16_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1402787.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31029..31901)
                     /gene="rsmA"
                     /locus_tag="VYN16_000197"
     CDS             complement(31029..31901)
                     /gene="rsmA"
                     /locus_tag="VYN16_000197"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1402788.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32032..32340)
                     /locus_tag="VYN16_000198"
     CDS             complement(32032..32340)
                     /locus_tag="VYN16_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402789.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32333..32974)
                     /locus_tag="VYN16_000199"
     CDS             complement(32333..32974)
                     /locus_tag="VYN16_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402790.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(32976..35006)
                     /locus_tag="VYN16_000200"
     CDS             complement(32976..35006)
                     /locus_tag="VYN16_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1402791.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35083..35379)
                     /locus_tag="VYN16_000201"
     CDS             complement(35083..35379)
                     /locus_tag="VYN16_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1402792.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(35699..36598)
                     /locus_tag="VYN16_000202"
     CDS             complement(35699..36598)
                     /locus_tag="VYN16_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1402793.1"
                     /translation="MLIGQKIKEIRIEKGVSRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(36670..37230)
                     /gene="rnmV"
                     /locus_tag="VYN16_000203"
     CDS             complement(36670..37230)
                     /gene="rnmV"
                     /locus_tag="VYN16_000203"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1402794.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLVGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37230..38003)
                     /locus_tag="VYN16_000204"
     CDS             complement(37230..38003)
                     /locus_tag="VYN16_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1402795.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(38299..38973)
                     /locus_tag="VYN16_000205"
     CDS             complement(38299..38973)
                     /locus_tag="VYN16_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1402796.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKPETTLGVDKGGNTGSSD
                     ANANAGGGSQAGGPAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENTKTMEDVKEAETIGVQAIAMVTVLKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVAATSGFALLGRKTR
                     RRKWNS"
     gene            complement(39236..39370)
                     /gene="rpmH"
                     /locus_tag="VYN16_000206"
     CDS             complement(39236..39370)
                     /gene="rpmH"
                     /locus_tag="VYN16_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1402797.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39515..40729)
                     /locus_tag="VYN16_000207"
     CDS             complement(39515..40729)
                     /locus_tag="VYN16_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1402798.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40885..41178)
                     /locus_tag="VYN16_000208"
     CDS             complement(40885..41178)
                     /locus_tag="VYN16_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402799.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            41340..41792
                     /locus_tag="VYN16_000209"
     CDS             41340..41792
                     /locus_tag="VYN16_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1402800.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41827..43215)
                     /locus_tag="VYN16_000210"
     CDS             complement(41827..43215)
                     /locus_tag="VYN16_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1402801.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43281..44243
                     /locus_tag="VYN16_000211"
     CDS             43281..44243
                     /locus_tag="VYN16_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1402802.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(44449..45459)
                     /gene="ccpA"
                     /locus_tag="VYN16_000212"
     CDS             complement(44449..45459)
                     /gene="ccpA"
                     /locus_tag="VYN16_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1402803.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(45884..46483)
                     /locus_tag="VYN16_000213"
     CDS             complement(45884..46483)
                     /locus_tag="VYN16_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1402804.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(46485..47582)
                     /locus_tag="VYN16_000214"
     CDS             complement(46485..47582)
                     /locus_tag="VYN16_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1402805.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGELPAWHLLVDILFVV
                     TYLGVLITKNQRISWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(47582..48319)
                     /locus_tag="VYN16_000215"
     CDS             complement(47582..48319)
                     /locus_tag="VYN16_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1402806.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(48321..49205)
                     /locus_tag="VYN16_000216"
     CDS             complement(48321..49205)
                     /locus_tag="VYN16_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402807.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(49192..49386)
                     /locus_tag="VYN16_000217"
     CDS             complement(49192..49386)
                     /locus_tag="VYN16_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402808.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(49383..49589)
                     /locus_tag="VYN16_000218"
     CDS             complement(49383..49589)
                     /locus_tag="VYN16_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402809.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14327 a        11357 c         9531 g        14589 t
ORIGIN      
        1 ttcacgaatg aagaaaaatc gtggaattca aaaattagct atattagtat tactaggtgt
       61 ttttatgttt agtaacacaa ttccttacca acagttattc agaagaatag acaattggag
      121 attcgagtgc aatcacaaaa gaagtccaat ggtcttgatg ttgggaaggc tgattgattt
      181 cgtattactt taaaattcaa ctatttagag aaaggtattc tgatgagagc ttgtaacaat
      241 accagataga tataagaatg gagatgtttg ataaaatgct ttcatagcta accaactgtt
      301 gaaaattatg agttacaaaa tctaaagtat ttaagattgg tttataaaat taggtctctg
      361 cctgtagaaa ggaacgtgca gttagaaagg aggaaaaaat aggatactat aattcagact
      421 gagatttcta attaaaatat gtagttgcat ttcgctcatt ttcataatat ttcatttaga
      481 ggagtaataa tatgttaaaa tttagaaaag tagttgcatc atcccttgcc gttttggcat
      541 ttagtttggc tcttcctgtt ttggcagttt ctcaccgtgg tggcgagtgg acctatggag
      601 gccatcatga tccatataat tggggagcat tttcgaatta ttatcatggt tcacagtatc
      661 actgggccta tgttggaagt aatgaacgtg acaatcaaaa aacagtttat gcaggagcac
      721 atagtgcggc ctatgcgttt gttaatacaa atttggggga gcgaattaca tttgatgcgg
      781 gatggtaaag aataattgaa gaagattctt ttatcaatag tttagtaagc ttgttagttt
      841 atagagggca ggggtatttc tgccctctgt tttagaataa ggaatgcact atgaaaaaat
      901 tatttatact catatccaac ctgcttgcta gtttgttctt tgtttgggta tttaccattt
      961 ggactgatac atatgtctcc cattattacc ccaacgttgt tgtacatgat tcttcccctg
     1021 aaacaacttt tcagcatgtt gctacacgct tggaaaaact agcagaagaa acagatagct
     1081 ttattgcgat tcaacaccaa gatcctaact cagaaggtac tacagttttt tcttatacga
     1141 cctttggaga cggaaagttg cctgacgggc tacaggaaaa aaaactagaa gatgctcaaa
     1201 gtagtagtgt tgaaacaaac tattttgtat tcgatggaca cctagatatt cacttgctaa
     1261 gggaggagct aagtcaactt ggcttgacta atatgcacct gacaattcca tctaaactat
     1321 ctacgttaat ggctatcttt agtaacggat ttcagttaat cagtctattg attttcatct
     1381 taacctttgg agcactgact ttaattagtc aaattagcca attacggtcg tcgggcattc
     1441 gcctcatttc aggagagaaa cggtggtcca tttttcttag accagttggt gaggatttga
     1501 aagggatagc tgttggtttc agtttagctg gcgtgctcac cattcttatg caaaaaattc
     1561 tgtcgcttcc aacgcaatcc ctaatgacca taggagcagg cttgctcagt tataatctca
     1621 tcttgttgtc tatctccctg tttttcgctc aactcttcgc agttgggata aaaaagatac
     1681 acctgatgca gattataaaa gggcaagtgc ctgtcagagg aatcattagt ttgattttaa
     1741 tcggtcaact attagcgatt attattgtaa cactgggaat agggagtagc ttaaagtatt
     1801 cccaagcctg gcaacagcat cggattggac aagaggcttg gagccaggaa aggcaactga
     1861 ttaccctatc aatcagccgt gaaggaacga gtcctggttt tgatgaacaa gctcaaagga
     1921 aactcagaac ttggtatcaa ttgatggatc tggctgtttc agaacaaaag gctttcttat
     1981 ctagacacca gttaattgac cgtactttgc aaaatggcat ggcttcctcc aaaaacttta
     2041 taacctctac agaatggcac gattacagcc cgaatggcaa tgtccttatc gtcacaccgc
     2101 aatacttgga gcgtcaaaac attcctgtag atacaactat tgagcaaaag atgaatcacc
     2161 ttgatgtagg ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagatcatt
     2221 ataaatctgt ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc
     2281 aaatgactgc tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga
     2341 cccctatttc ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat
     2401 caactggtcc acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta
     2461 atcaattgtc tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag
     2521 aaatgcaaac aggttaccac aactacatca cattattgga taatatccag agggaacgtt
     2581 gggtaatgct agcaggagct gtgcttggga ttgcaacttc aatcttgctg tttaacacta
     2641 tgaataggct ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc
     2701 tcaggttctt agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg
     2761 gatttgttgc tagtgtattt cttcaggtag aaataggagt tgctttctta gtcttgttac
     2821 tctttactgg tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt
     2881 ccatgcttgt tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt
     2941 ggagaacgag agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc
     3001 ttaattggtt caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa
     3061 ccttatgatg ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcgagtgat
     3121 tttttccgtc acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt
     3181 attgaagaaa accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg
     3241 ttgaggcaga agcaggcttt agaacaggtc ggcctggctt atcttgacct agataagcgc
     3301 atctttgagt tatcgggcgg agaatcgcaa cgagttgcct tggcaaaaat tatcttaaag
     3361 aatccaccct ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag
     3421 ttgattatgg agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca
     3481 cataatccgg caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa
     3541 aaatccttgt ttttaattgc acgatgagtt actgaaatat tatcatgaat caagaattgg
     3601 agttaattta gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaggaa
     3661 atagtggaga ggaagttaga atgaaaaaat tcgacaatta tattattgag aagccttgcg
     3721 attctaattc agataaactg caaaaaatct taataattga aaatttggta gatgatattt
     3781 tgcaatttcc tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttc
     3841 aaaagaaaac tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg
     3901 atgataaagt tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt
     3961 aatctgtgtt gaaggctcaa aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc
     4021 caaagattta gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat
     4081 gttgtttcag tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa
     4141 aaagtataga aatatagtca attgaaacaa gaacaggaca aaagaacctt ttgtgccata
     4201 tttttctcct ttcgctttac aattggcttg aacaccttta ttgtatcgcg tttggagttt
     4261 ttttggtata accttcgacg cgcacccgca tagcgggtgt ttttttgtct cgcacctaac
     4321 ggagcgagac gaactaatag tcacttaatc aaaaaaacgc accatatcaa aaactaaaaa
     4381 gtttgatatc atgcgtcatg tcttaaacta attgactata ctttctattc aaatgagctt
     4441 ttaaccaatt gattgagcca atccactctt aaaaccaaag agcaatttct cgcttagctg
     4501 actcttctga atctgaacca tgtacaacat tttggataac ctcattttct ccagcagctt
     4561 ttgcaaagtc acctcgaata gtgcctggta aagcttcttc tggacgagtt gcacccatca
     4621 tggtccgcca agtttcgatt actttgggac cagaaatgac acccacaaga actggacctg
     4681 aagtcatgaa ttcacgaatc ggtaggtaaa aactctgacc aaccaagtcc tgatagtgct
     4741 ggtcaatcaa ctcttctgaa acctgtgaac gaaactccaa tttttcgatt gtaaatccac
     4801 gttgttcgat gcgctttaac acttcaccca ctagccctct ttttacacca tctggtttga
     4861 tgataaagaa tgtttgttcc atacccgtct cctttgtcag cttctttctt ttattttacc
     4921 acatttcgtg gaaaaatgga gaaagttttc agaagagaga atgagagaac ccgagggttc
     4981 tctcattctc tcttattcta ctgtttcttc cacagtttca acggcagtat ccacaactac
     5041 ttctgttgtt tcttcatttc cttcttcctc tactgtagga ttaaggtatt cttcttcgtt
     5101 gacagcatgt ggttcaaggt tacggtaacg ggccatacca gtaccagctg ggatgatctt
     5161 accgatgata acattttctt taagtccaag gagatggtct ttcttaccac ggatagctgc
     5221 gtcagtaagg acacgagttg tttcttggaa ggaagctgct gacaagaaac tgtttgtttc
     5281 aagtgaggct ttggtaattc ccataaggac tgggcgacct gtcgctggaa ctccacctgc
     5341 gataaggaca tctttgttgg catctgtaaa gtcattgata tccatgaggg tacccatgag
     5401 aagatctgta tcacctggat ccatgacacg gactttacgg atcatttgac gaaccattac
     5461 ctcgatgtgt ttgtcaccga tttctacccc ttggctacgg taaacttttt gtacttcacc
     5521 gagaaggtac gtttcaactg acaagacatc acgaactgca aggagacgtt ttggttggat
     5581 agaaccttct gtcagagcag caccacgcgc tacttggccc ccaacttcga cacgcatacg
     5641 agctgtaaat ggaacgacat attcaccttc gccagtttca cccttaacaa agactttctt
     5701 ggtacgagtt gatgcatctt cttcgatagc agtaacttgt cctttaacct ctgtgataac
     5761 cgcttcccct ttaggattac gggcttcaaa gatttcttgg acacgaggaa gaccctgagt
     5821 gatatcggta tttgaggcaa ccccacctgt gtggaaggta cgcattgtaa gctgtgtacc
     5881 aggttccccg atagattggg cagcaattgt accaactgct tcaccaactt caaccgcatc
     5941 accagtcgcc aagttgatac cgtaacagtg acggcagaca ccgtgacgag tgttacatgt
     6001 aaatacagaa cggatagtca cttcttccac accagcattg acaatttcac gcgccttgtc
     6061 ttctgtaatc aattcatttg gaccaataat cactgcacca gtttctggat gtttaacagt
     6121 tttcttagtg taacgaccgt tgagacgctc ttcgagagac tcgatcatct cttttccttc
     6181 tgcgatagaa cggatcaaga gaccacggtc agttccacag tcgtcctcac ggatgataac
     6241 gtcttgggca acgtcgacca aacgacgagt caagtaacct gagtcggctg tcttaagggc
     6301 cgtatcggtc atacctttac gagcaccgtg agttgagaag aacatttcca ataccgacaa
     6361 accttcgcgg aagtttgaaa ggattggcaa ttccatgata cgtccattcg gagcagccat
     6421 cagaccacgc ataccggcaa gctgtgagaa gtttgagatg ttaccacggg ctccagagtc
     6481 catcatcata acgattgggt tcttaggatc ttggttagca atcaagcgtt tctcaagttt
     6541 ttcacgggca gcacgccatt cagctgtaac agcattgtaa cgctcgtcgt ctgtgatcat
     6601 accacgacgg aattgtttgg tgatttgttc gacacgtttg tgtgattctt caatgatttc
     6661 agtcttgtca tcaacgactg ggatatcggc aatacccact gtcaatcctg caagagttga
     6721 gtggtggtaa ccgaggttct tcatgcggtc aagtagggca gaagtttctg tcgtacggaa
     6781 acgtttgaag atttcagcga tgatatttcc aaggtttttc ttcttgaatg gagggttgag
     6841 ctcaagattg ctgatagctt ccttgatatc tccaccaagt ggcaagaagt atttagctgg
     6901 aacaccttct gtcaagttgg cattgtttgg ttcttgcaag tatggtagcc cctctggcat
     6961 gatatcgttg aagagaattt taccaactgt tgtaagcaag accttatgtc tttgctcttc
     7021 tgtccaaggc ttgttgaggc tgtctgttgc gataccaaca cgtgagtgga ggtgaacata
     7081 accattgcgg taagccataa ccgcttcgtc acggtctttg aagaccattc cttcaccttc
     7141 gcgaccagct tcttccatgg tcaagtagta gttacccaaa accatgtcct gagatggagt
     7201 aactaccggt ttcccatctt tcgggttcaa gatgtgctca gcagctagca tgaggatacg
     7261 agcttctgct tgtgcttctt ctgaaagtgg tacgtggatg gccatttggt ccccgtcaaa
     7321 gtcagcattg taggcttcac agacaagtgg gtgcaagcga agagccttac catcaattaa
     7381 gactggctcg aaggcttgga tacccaaacg gtgaagggtc ggtgcgcggt tcaaaagcac
     7441 tgggtgttct ttaatcactt cttcaaggat atcccagata cgctcatctc cgcgttccac
     7501 caagcgttta gctgctttga cgttttgcac gatatcacgg gcaacgattt cacgcataac
     7561 aaatggttta aagagttcaa tcgccatttc acgtggcaca ccacattggt acatcttaag
     7621 agttggacca acggcgataa cggaacgtcc tgagaagtca acacgtttac cgagcaagtt
     7681 ttgacggaag cgtccttgtt tacctttaag catgtggctc aatgatttca atggacggct
     7741 acctggtcct gtgattggac gaccacgacg accattgtca atcaaagcgt caactgcttc
     7801 ttgaagcata cgcttctcat tttgaacgat gatacctggt gcatttaact caagcaaacg
     7861 agccaaacgg ttgttacggt tgataacacg gcggtaaagg tcattcaagt cagatgaggc
     7921 aaaacggcca ccatccaact gcaacattgg acgaagatct ggtgggataa ccggaaggat
     7981 gttaagaatc atccattcag gtttgtttcc agacttgtaa aaggcatcca aaacatccaa
     8041 acgacggatg gctttgacac gtttttgtcc agtagctgtt ttcaattctt ctttgagttc
     8101 agcaatttct ttttcaagat ctacttgctt caaaaggtct tggatggctt ccgcacccat
     8161 cttggcaaca aatgaaccat aaccatattc acgcaagcgc tctcggtatt cgcgctctgt
     8221 catgatagac ttgtgctcaa gtggtgtatc cttaggatca atcaccacat aagccgcaaa
     8281 gtagataact tcctcgaggg cacgagggct catatcaagg gtcaagccca tacggcttgg
     8341 aatccccttg aagtaccaga tgtgagatac aggagctttc aattcgatat gtcccatacg
     8401 ctcacgacga actttcgtac gcgttacttc aaccccacag cggtcacaaa caattcctct
     8461 gtaacgaatg cgtttgtact taccacaagc acattcccag tcttttgtag gaccaaagat
     8521 cacttcatca aagagtcctt cacgttctgg tttcaaggta cggtaattga ttgtttcagg
     8581 ttttttgact tctccataag accatgaacg gactttactt ggagaagcta gggtgatttg
     8641 catactttta aaacgattta catcaaccac tatttcttcc ctttctattc taagtgaact
     8701 gcttattctt gttcagcagc ttcttctgtt gcttccgctt ttgttgcttt ctcagcttct
     8761 tcagcttcaa aggctgcttt agcctcttgg gctgcttttt cgcgggcttt ttcaaggtca
     8821 tctacgtgga tgacatcttc gtccattcct tcatccaagt cgcgaagttc cacttcttgg
     8881 tcatcttcgt ctaggacacg catgtcaaga ccaagagatt gcaattcttt gacaagaact
     8941 cggaaggatt ctggaacacc tggttttgga attggtttgc cttttgtaat agcttcatag
     9001 gctttcaaac gtccgttgat atcgtcagac ttgtaagtca agatttcttg aaggacattt
     9061 gacgcaccgt aggcttcaag agcccaaacc tccatctcac cgaaacgttg tccaccaaac
     9121 tgagctttac ctccgagtgg ttgttgggta acagttgagt aaggtccgac tgaacgcgcg
     9181 tgcaatttat cgtcaaccat gtggtggagt ttgatcatgt acatgactcc aacagaaaca
     9241 cggttatcaa atggttcacc agtacgtcca tcgtaaagga tcgttttggc atcgctatcc
     9301 atacctgctt ctttaacagt tgaccaaaga tcttcagaac ttgctccatc aaagactggt
     9361 gtcgcaatgt gaataccaag agtacgagct gccataccaa ggtgaagctc cataacctga
     9421 ccgatattca tacgtgatgg caccccaagt gggttcaaca tgatgtcgac tggagttccg
     9481 tctggaaggt aaggcatgtc ttctacagga acgatacgag agacaacccc tttgtttccg
     9541 tgacgtccgg ccattttatc tccgacctta atcttacgtt tttgagcgat gtaaacacga
     9601 accaacatgt taacacctga ttgcaactca tctccattta cacgtgtaaa gatcttaaca
     9661 tcacgaacga caccatcggc accgtgtggt acacgaagag aagtatcacg cacttcacga
     9721 gacttgtctc caaagatagc gtgcaagaga cgttcttcag ctgaaagatc tttctcaccc
     9781 ttaggtgtta ctttacctac aagaatatca ccttctttaa cctcagcacc aatacggata
     9841 atccccattt cgtcaaggtc tttgagggca tcttcaccaa cgtttggaat ttcgcgagtg
     9901 atttcttcag gcccaagctt tgtatcgcgc gtttctgatt cgtattcttc aaggtgaaca
     9961 gatgtgtaga catcgtcctt caccaagcgt tcgctcatga taacggcatc ctcgaagttg
    10021 taaccttccc aagtcatgta ggcaacgatt gggttttgtc caagcgccat ttctccattt
    10081 tccatagaag gtccgtcagc gatgaaatcg cctttttcaa cgacatcacc aacttttacg
    10141 agagtgcgtt ggttgtaagc agtacctgag tttgaacgac ggaatttttg gatgtggtaa
    10201 acatccaatg aaccatcttc acgacgaact tctaccttgt cagcatctgc gtaagtaact
    10261 ttaccatcat actgagcaat cacagccgca ccagaatcgt gggctgcttg gtattccata
    10321 ccagtaccaa cgtaaggtgc ctgaggatta atcaatggca cagcctgacg ttgcatattg
    10381 gctcccatga gggcacggtt ggagtcatcg ttttccaaga aaggaataca tgctgtcgca
    10441 acggcaacta cctgttttgg tgaaacgtcc atgtagtcaa caatattagc tggatactct
    10501 tggttgaccc cttggtgacg tcccatgaca atcttctcag caaaggttcc atcttcattc
    10561 agacgagagt tagcctgagc tacagtatat tcatcttctt catcagctgt caaccaaaca
    10621 atttcgttcg tgacaacacc tgtttcacgg tcaaccttac ggtatggtgt ttgaacaaaa
    10681 ccatatttgt tcaagtgtcc gtaagatgac aagttattga tcaaaccgat gttaggtcct
    10741 tcaggtgtct cgattggaca catacgacca tagtgagtgt agtgcacgtc acgtacttca
    10801 tatccggcac ggtcacgagt caaaccacca ggtcctaagg ctgacaaacg gcgtttgtga
    10861 gacaactcag aaagcgggtt gtgttggtcc atgaactgtg acaactgtga tgaaccaaag
    10921 aattctttaa ctgcagctgt tacaggacgg atattgataa tttgttgtgg tgtcaagact
    10981 tcattgtcct gaacagacat acgttcacgg acattacgtt ccatacgaga aagtcccaaa
    11041 cgtacttggt tggcaagcaa ttcaccaacc gcacggatac gacgatttcc aaggtggtcg
    11101 atatcatcta cacggccaag tccttcagcc aagttgagga agtagctcat ctcggcaagg
    11161 atatctgcag gagtcaccgt acgaaccttg tcatctgggt tagcattacc aatgatcgtt
    11221 acgacgcgat ctggatcagt tggagcaaca accttgaatt tttgaagaac aacaggctca
    11281 gtcacaacgg ctgcatcgtt tgggatgtag acaatcttgt tcaagtcgcc atccaaatgg
    11341 ctttcaatgc tttcaatcac gctacgagtc ataatcgtac cagcttctac caagatttct
    11401 ccagtttcag ggtctaccaa tggctctgca atggtttggt tgagcaaacg tgttttaaca
    11461 ttgagttttt tattgatttt gtaacgacca actgctgcca agtcatagcg acgtgggtca
    11521 aagaagcgag ccacaagcaa gctacgtgag ctttcagctg tcttaggctc acctggacga
    11581 aggcgttcgt aaatttcttt caaggcttcg tctgtacgag agtccattgg attcttgtgg
    11641 atatcttttt caacagtgtt gcgaaccaat tcgctgtcac caaagatatc aaagatttca
    11701 tcatcacctg agaaaccaag agcacgaacc aaggttgtaa atggaatctt acgagtacgg
    11761 tcgatacgag tgtaggtgat atcttttgag tcgctttcaa gttccaacca agctccacgg
    11821 ttagggataa cagttgaacc atagcccacc ttaccatttt tgtctacttt gtcgttaaag
    11881 taaacacctg gtgagcggac caactgagaa acgataatac gttcaccacc attgatgatg
    11941 aaagtaccca tttctgtcat gattgggaaa tcaccaaaga aaacttcttg ggtcttgatt
    12001 tcgcctgttt ctttattgat caaacggaag gttacaaaaa ttggtgctga gtagctagca
    12061 tcgtggatac gagcttcttc tagcgtgtat tttggttcct tgatttcata tccaacaaat
    12121 tccaactcca ttgtgtctgt gaagtttgaa attggcaata catcttcaaa cacttcctta
    12181 agaccgtggt ctaggaaagc tttgaatgag tcagtttgaa tttcaatcaa atttggtaag
    12241 tcaagaactt ctttgattct tgaaaaacta cgacgggtac gatgtttccc gtattgaacg
    12301 tcatgtcctg ccaagatgat tctcctttgt aaataagttc caagccttgt caatcaggct
    12361 tttctaatcg ccatatggtt gtaaacccct tatcaccgtg tcctcttgac gaattttcag
    12421 aatctttaag cctctgttac aaatgctcaa aatcttgaaa aaaagcacaa aaagagcagc
    12481 taaatctgac tttttcagaa gatttaactg ctgtgagcct tgtctggaca atatttcaga
    12541 caaaacctac gacaaatgat tacccatatt ataccctatt tagctagatt tttcaagggg
    12601 tttcagtagg tttttggtaa attttttccc atagaaaact tggcatcaca ttcgaatcac
    12661 gctatggtac aaaaaactga aaaaactatt gactgaaaat cattttcaag gtataataat
    12721 aaacgttaag gcggtatagc caagtggtaa ggcacggctc tgcaaaagct tgatcgtcgg
    12781 ttcaaatccg tctaccgcct tctataactt gatttatcag gtttcaaatg aacagaaagc
    12841 ccaatttgaa gggcttttct tattttccct cgaataaata cgtataactt taaaaacttt
    12901 tggagcgagt ttgtggcaga gttctttcca tggcataatt cccttttgaa atcaattgaa
    12961 aaaagcttcg tcacgcctgg tgacaaaagc ctctagttta ttctgtttct tcttctattt
    13021 cgacttctgt gatttggatt tttaccttgg taacacgtcc attcttcact ttatcattgg
    13081 ttaagataaa ctgtttgttt tggctgatca attcatagct gagtttctca gtcgttggaa
    13141 tggttcccac tcctgtcaaa taataaccag cgatagtatc aacgtcatca ctttccagtt
    13201 caacattaaa gtaattattg aaatcattga gggtcatagt tccttgaaca atgtaggtat
    13261 cctcgccaat ctgataaaca tctattgctg ctttgtccgt ttcatcatca atttcaccaa
    13321 caatttcctc taagaggtct tccaaagtca ccaaaccagc cataccaccg tattcatcca
    13381 gcaagattgc catttgtctt tgggtatttc gcagttcttt tagcaagtca tccacaaaaa
    13441 tagtttcagg tacaaaaagt ggatcttgta aaattctctt ccaaacaata ttgtcaaaac
    13501 cgtccacaaa gcctgcctta aggagactct tggtgtgaat gattccaatt acattgtcct
    13561 tatccccatc ataaaccggg atacgagaat aattttgttt taaaatactt tggataatgg
    13621 cttgactatc atcctgaata tccaccataa aggcatccgt tcgaggaacc ataacctctc
    13681 gtgccatcag ttcatcgagc gaaaagacac cttgtagcat ctcaatctca tcagcatcca
    13741 atgtttcttc actatttgtc agcatatagg caatttcatc acgggtcatc ttttcatccg
    13801 catcatcgaa tgacatagga gtcaaatggc tcaagaaatt ggtcgaagca gctaaaagcc
    13861 aaacaaaagg actgactagt tttccgatcc caatgataac cggcgctgta cgaattgcca
    13921 aggcatcctt tagattaaga gcgattctct taggatataa ttccccaaaa acgatggaaa
    13981 tataggtcaa aaatgccaag gatagaaaag ttgccacggc ttgtgctgtt tcgccattcc
    14041 caagccaaga ggcaatcaca cgtcctagag tatcagttaa actcgcccct gataagattg
    14101 taatcagggt gattcctacc tgaatggttg ataaaaagtg gttaggattt tctagtacct
    14161 tcagcaggcg gatgtagcgt ctgtctcctt cttccgcctt ttgttcaact cgggcacgat
    14221 taagagaaac catggccatt tctgtggctg agaaaaaggc atttaatacc gtcaagataa
    14281 acaataaaac aaattgtagc aacaaattct gactacttgg gtcttccata gttcttctcc
    14341 taaaatagta tcttatcctt tattatatca caaaatttaa tccaatacat attatttttt
    14401 tcttagcgta acttccatct ctctcccaaa actggaagtt agtctaagac ggatttccac
    14461 tgatttcacg cgtttttctc atactaaaag ctaggttgtg ggaaactgga agttagtttt
    14521 agaagttgct attttttctt agtacctgtt ctagccctgc ttaaactagt aaaaagaact
    14581 cctacatcca taagtgacat aggagtttag gcttatcttt tactgagtta cagttacttc
    14641 tccagttcgg taatccagtt gaagtccctt ttgaacattg ggaactagaa cattgaattc
    14701 caattctcca tccgaagact tggcttcaat ctgtgaagct gagggaacta aatcctcgtt
    14761 tgaagcgtag taaagggtta cacggtaacg gacacgcttg ttttggtcca aggctttacg
    14821 caccttgctt tcatagtagt tttgaccagt cgaatactcg gcttgtgcct gatttgccca
    14881 ggctgtctga acagcaatgt ttttaggatt gcttgttgag gcatcaaaac catccaaacc
    14941 accgattaag gcatagccta acaaatgacc tctatcgact gcatgggtat aagagccctt
    15001 tagattcttg acctgatgcc aacctggagg agtccaagaa gttgaaccat tcccagtttc
    15061 tttacgattc ttgtactgac gagtggcatt agacaagagg gcattagcta cggttggaac
    15121 agtttccttg cccactgtct ttgttttatt gtcagcgtag ggcttacttg aaaccttggc
    15181 atctagattt gttttattac cattgacgat aaaagcacct gagccattcc actccagact
    15241 cccctttatt tgactcttga ctgcgtctgt taagacactc tctgccaatg cttgactagg
    15301 agcttcaggc gcttgttttt tctgactaag attggttttg ggactattag gtgccgactg
    15361 catctgcttg atataatagc tccctgtaga caaaagcaat aacactagca gtccgattag
    15421 tgtctgtctt gtttttttgt tcatatttct ccttatcttt agaaaaaggc tggttctcca
    15481 gcctaatttc ctgtaaattt atgaatcaat tcctgccatt ttgctggaga caggatagcc
    15541 catggatctt gacccttgcc caagattcca taacctacca ttagaccgat tcctagagcc
    15601 agagtaccta aaatcagtac tatgatgact aaaagtaaac gcttgactac gtagcttgat
    15661 ttcttattca tcttcatcac ttgcctcaat ccgctgaatc ttagcaccta gctgcgccaa
    15721 cttctcatgg aaaccgtagt aacctctatc caagtgaacc aatttaccga ccacagtttc
    15781 tccctgtgct accaaacctg tcaaaatcaa agctgcactg gcacgaaggt cagttgaaag
    15841 aacttctgct ccctgcaaag gctgtccacc aacaatacga gctgtatcac ggataatctc
    15901 agaatgcaag cccatgcggc gcatctcttc taggtgttgg aaacgatttt caaaaactgt
    15961 ctccaccatg gttgattcgc cttttgcaac tgtcatcaag gctgtaaatt gagcctgcat
    16021 atctgttgga aatcctgggt ggggcaaggt tttcacatga acagctttta gattttccag
    16081 ttgagaacga acacgaattc cttcgtcttc ttcaattact tcaacaccca tttcaagtaa
    16141 cttggcaatc aagggacggt tgtgctccca gacagcgtct cgaatcaaga catcaccacc
    16201 agtcatggca gcagctacca taaaggttcc tgcttcgata cggtcctgga ctacattgtg
    16261 agtcgtacca tgaagtttct caacaccagt aatggttata gtctctgtac cagcaccttt
    16321 gaccttggct cccatttcat taaggagaat ggctaagtca acaatctcag gctcacgcgc
    16381 agcattctca atcactgtca ccccatcagc cagagtcgct gccatcatca agttctgcgt
    16441 tgcaccaaca cttggaaagt ccatatagat atgagcacca tgcaagcgtt ctgccttggc
    16501 ttcgatgtaa ccagctgtct gactaatctt aacccccata gcttccagac ctttcaaatg
    16561 aagatcaata ggacggctac caatcgtaca accacctggc atggatacct tggcatgacc
    16621 cacacgggca aggattggcc ctaatacaac gatggaggcg cgcatcttac tgacatactt
    16681 gtaaggggct tcctcagtga tgtcgccagt agcatccacc ttgacaagat gagcttcctc
    16741 atcaaagtca accttggcat tcaaaccacc aactacctga ttcataataa agacatccga
    16801 caaaatcgga acattctgca agacggtctt tccttcactt gctagaatag tcgctgccaa
    16861 caagggtaag actgcatttt ttgctccctc gatcgtcacg cttcctacca gacgattatc
    16921 gccaccttga accacaattt tatccatact cgtttccttt actcttgatt ttataataat
    16981 cctctaagcg cttcttgcca caaatgatac aggctaataa agaaagaact caggatgtag
    17041 cctatgacaa tgctaaaaaa ggctactaat acacgaactt ttcttgtatt ctcagctgtc
    17101 acttttaaaa ccttttccca tctaacaaga tcctttaaaa ggtaaaaact cacataaata
    17161 aagagcatat gactacttag agtgaataat aattgaacca tttgactatt ataccatctt
    17221 ctctgtttgt gcgctagttt ggaaacttca ctcaaaaatt agggattgtt tttcaagtaa
    17281 tcaatcgcat cttgtagggt tttaacgggc acgattttca tatccgtctt gattgtttta
    17341 gccgcttcta gggctgtttg gtagttgttt ttcgcgtccg gatgcgcctt ttgttcttct
    17401 tcgctaacag ggttatcagg agcaaagaaa atagcagcac ctgccctagc cgaagctaca
    17461 accttcttat caatacctcc aatgtctccc acattaccat cgcggtcaat ggtacctgta
    17521 ccggcaacaa tacgaccatt acgaagatct gggtgagcta tttgagtata gatagctaga
    17581 ctaaacatga gaccagcact tgggccgcca ataccagctg ttgaaaagct aattgggaca
    17641 ttgctgatta cctctgtacg atcaatcaag ccgattccaa tcccattttt tccattttcc
    17701 aaggtgataa tctttccttc tgcagacttg gtttgcccat cctcttcata ggtgaccttg
    17761 acggagtccc ctaatttttg agaactgacg taatcaatca agtctttgga actatcaaag
    17821 gtctgatcat tgactgctgt gactgtatca gagatattga gaatcccttt aaaggttgaa
    17881 ttatccgtca cattcaaaac ataaactcca aagtacttga gttcgatatc cttaccagct
    17941 gtttttagtc cttgatactt ggccatattt tgcgatgttt gcatgtagaa ttgattgatt
    18001 cgcataaatt caacatcgga agaaccacct gtagtctcct gagcactacg aatatctgta
    18061 aaaggtgtca accaagcata aatcatatga gctaaagtag catgttgaac accaaccgta
    18121 acgaattgat aggcaccagc ttccttatct tctgtgtcat ttactttaag gacttggcga
    18181 atatcttccg aaccacctgg aacctctata tagtagggca aaggcaccac aaatgccaag
    18241 aaggtcacaa tcaaggccgc aatgacatac aagggccatc taatcttttt tttcatttct
    18301 tatttcctcc aaaatactct cgggaacatg gcaggcaata tcctgaccaa acttcaaaag
    18361 ctctcgaaca cctgatgaac tgatatagag atgttcaggc cggctatgta aataaatagt
    18421 ctctatatca gaagacagct gatgattgta gtaatcaaaa ctagcttcat attgcaaatc
    18481 cgccgcattc ctcaaccccc gtactaggaa agtagccccc agtctttttg caacatcgac
    18541 caccaattta tcatgagaag ccacgacttc aacattttcc aaatgtccca aagccttttc
    18601 tagcccccgt ttacgatttt caagagggag aaatccttgt ttgtggggat taaaaaaaat
    18661 acccacataa agcttatcaa aaagtctgct cgcccgttca atgatatcca gatgcccatt
    18721 tgtcatcgga tcaaatgagc ctgtgaataa gccaatctta tctgacatag actgtcacct
    18781 tactaattcc ataaatcttt tccttccaga tacccagaca ggcaatttct tctggaagtt
    18841 caacggcttt atccgtctcg cacacaacca taacatcttc agaaaaaagc tctctctcag
    18901 ccattttttc aatatctgct acgatttgtt ccttggcata gggagggtct aagaaaacga
    18961 ggtcaaattc cccagatacc tgttccaatg ccctttctgc atccattttg aggagttgaa
    19021 attttccaac ttccttggtc atctggatat tttcagccac gatggtctga gccttacggt
    19081 ctcgctccac caaaacagca ctggacatgc cacgcgatac tgcttcgata gataaaccac
    19141 cactacctgc ataaaggtcc aagactcgtc ccccttcaaa gtagggacca atcatgttaa
    19201 aaatggctcc cctaacctta tccgaagtag gtcttgttgt cttgccttct agtgtcttaa
    19261 ggggacgtcc cccatagatt cctgatatga ttttcatact gtttattata ccaaattata
    19321 gacaaaaaga gaaagaaaac cgaaccttgc ggttcgattc tctacaaaat attttcgtaa
    19381 gtatcgcgga cttcttgagg ccaaacactt gtttgcactt ctccgatgtg tctcttgcga
    19441 agtaggaaca tggccatacg agattgtcca attcctccac cgattgtcaa tgggaatagg
    19501 ccattcaaca aagacttgtg ccattccaat tccaagcggt cttcatcacc tgtaatttcc
    19561 acctgacgtc taagagtttc ttcatctaca cgaattccca tagaagacaa ctcaaaggct
    19621 ccacctaaag actcattcca gacaagaata tcaccattta gacccttgta gccattctca
    19681 gactcgcttg tccagtcatc atagtctggt gcacgtccat cgtgcggttt accatctggc
    19741 aactcgccac cgataccaat caaaaagacg gctccaaatt ctttacaaat cgcattttca
    19801 cgttctttcg gtgtcaagtc tgggtagcgt tctaccaatt cttctgtatg gataaaggtg
    19861 atttgttttg gcaagataga ctcgatgtca tagcgggctt caacagctag ctcagtcagg
    19921 cgaatagcct tataaatctt ctcaactgtt tcttttagat aaacgatgtt acgcttacca
    19981 tttgggataa ccttctccca gtcccactgg tcaacataaa cagagtgggt tgcatccaag
    20041 gaatcctcat ctggacgaag ggctttcatg tggacaaaga gaccctctcc ttcaccaaag
    20101 ccaaaacgag ccaaggtgtg gcgtttccat ttagcaagtg agtgcaccac ttcataagta
    20161 gcatcaggga tttggagaac cttgaccgat actggatttt ccacaccaga caggttgtcc
    20221 tgcattccgt caccgacctt actcaagata ggaccttgaa cttcgacaac ttctagctta
    20281 tctttcaaat actgggtaaa agtgtttttg acaaaggaaa tttcttcttg ttgatggata
    20341 aaacttttct tcatagacaa ctcctcaaaa atttaataaa cgtattatac acctatttcc
    20401 aagaaaagaa aagagaaaat ttaaaaaaaa atcagtgtca ctcaaaacac tgatttcata
    20461 gaccttttag tcattgcgac caaagatacg tagaatactt aggaaaagat tgataaaatc
    20521 aagatagatg ctgagagcca ttgacaccac ccagcctgtc gctacacgac cttgtgattg
    20581 ttcataggcg aggcgaatct tttggttatc ccaagcaatc aaccctgaaa aaaccaaaac
    20641 catggctacg ctaatcatat aatcaaagaa gccactagcc aagaaaatat tgactaccat
    20701 agcaatcagt aaaccaatga gagctgccat cattgctcga ccaatcccac tcaagtcttt
    20761 cttggtaaag ataccaactg ccgccatgac aaagaagaga agggcgctcg atacaaaggc
    20821 agataaaact gtacccggag tatagaaagc caccacaaaa ctaagggtaa agccgtttaa
    20881 aacggagtaa agtaaaaata ctggaagagc agctggacta tttcttgaag ccatactact
    20941 ggcaacgaaa accaaagcca actccgcaaa agtagcaatg gtcaaccaga gacgcccctg
    21001 catcaacaag taaaccaact gagactgaaa gaccgtcaac ataaggcctg ataccaaagc
    21061 agatagtccg attcccagac caacaaaggc ataaacctta gcgtaaaatt gattgagacc
    21121 tgcacggtca tgtataatag tgtgattcat ccttttctcc ttttctgtga acatctttcc
    21181 ttgattataa caaaaaaagt taaaattagc ttaaatggaa gattaaaata ccggctgcaa
    21241 ctgcaacatt caaactctcg gcctgcccct tcatgctaat atggaccaac tggtctgcac
    21301 tttcagccat aaggggacta attccttgac cctcatttcc cataactagt acaaaatttt
    21361 ctatagaagg cagttctctg taatcaacag aatctttaga tagggttgtt gctagcactg
    21421 ggatagctac ctttttagtt tcgtcaagaa gcgcttgact agtcatccgg taaatgggca
    21481 gatggaaatg actaccttgc atggaacgta gggttttgag actgtagatg tctgccgact
    21541 tatctgaaac aatcactcct gtaaaacctg ctgcatccgc agttcgaatg atagttccta
    21601 cattaccagg atcttgcaca tcttccaaaa acaagaactt gccctgactc aaatcagctt
    21661 gtcctacttc ttctttttga accaccgcca caattccctg tggagtctga gaatccgcca
    21721 aatctagcaa aatatcctct gaaatccaga cagtttgcgg aaaagcagct aactgatctc
    21781 gataattttc tagggcaaag attttctcaa tcgtcactcc agcttggaca gcctcttcaa
    21841 acaagtgcca gccttcaatc aaataggcag acttgcggta ttttttttgg tgtaatttct
    21901 tggcattttt taccacagaa ttggcttttg aggttataat agtcatagaa atattataac
    21961 acaatcaaag gggtttggta tgcaaaaggt tagaatgatt gcccaaggta gggtacaggg
    22021 agtcggcttt cgttggggtg tttacagctt ggcacttgaa attggtggca tcacaggtcg
    22081 agtatggaat aacgacgatg gcacagtgga aatcttagcc caagcagact catctgctat
    22141 catggcaaaa tttatccaag aaatccgaaa aggaccgaca cctttttcaa aagtaagcta
    22201 cttagatgtc aaactaagca actttcctcc ctactctgac tttaaaatcg caaattaggt
    22261 ctccagaact attgtatatt ttgtaaaaaa acagtagaat agaaaggtat aatttttaaa
    22321 gaaggaataa aaacagtgaa atctattaaa cgttttgcat tctcggctat gggagtggct
    22381 atgttgcttg tcttgactgg ctgtgtcaat gtcgataaaa ccacaggtca gccaacagga
    22441 tttatttgga ataccatcgg agcgcctatg gctgaagcca tcaagtactt cgctactgat
    22501 aaaggtctag gctttggtgt cgctatcatt atcgtaacca ttatcgtacg cttgattatc
    22561 ttaccacttg gtatctacca atcatggaag gcaacgcttc actctgaaaa gatgaacgcc
    22621 ctcaagcacg tccttgagcc acaccaaacg cgtctcaaag aagcgactac tcaagaagaa
    22681 aaactcgaag cccaacaagc tctctttgct gctcaaaaag agcacggtat cagcatgttt
    22741 ggcggtgtag gatgtttccc tctcctcctt caaatgcctt tcttctctgc tatctacttt
    22801 gctgcccaac atactgaagg ggttgctcaa gcaagctacc taggcattcc tctaggttct
    22861 ccaagtatga ttttggttgc ctgtgctggt gtcctttact atcttcaatc gctcctttca
    22921 cttcacggag tagaagatga aatgcaaaga gaacaaatca agaaaatgat ttacatgagc
    22981 ccactcatga tcgtcgtctt ctccctcttc tcaccagcta gtgtcacact ttactgggtt
    23041 gtcggtggtt tcatgatgat tctccaacag tttatcgtca actatatcgt tcgtccaaaa
    23101 cttcgcaaaa aagtccgtga agaactggca aagaacccac caaaagcaag tgctttctca
    23161 acaccaagtg gacgaaaaga cgttacccct gaacaaccaa ctgctattac aagcaagaaa
    23221 aaacacaaaa atcgcaacgc tggaaaacaa cgttcgagat aagaagaaaa aggcttagct
    23281 gatttgctaa gtctttttgc aagtcaaaag cccgatgttt ccatcaggct tcttttctat
    23341 ccatgtacac gtttcatata atcttgataa ctttcggtat ccatgagttg tttagcgttc
    23401 ttgacgcgat ctgctgttgg tggtttgact ccttcgaggg aatatggaat tccaagttca
    23461 cgccacttga actcacccat ggtgtgataa ggtagaattt caaacttatc aacatttttg
    23521 agggtcttga cgaacttacc aagttcaatc aagtcatcat ctctgtctgt caatcctgga
    23581 actagcacgt ggcgaatcca gacaggtttt ccaatatctg atagatactg ggcacaagcc
    23641 aagatatttt tattggtttg gctagtgaca atcttgtgct gttcttcgtt gatttccttg
    23701 atatccaaaa gaaccaagtc agtgacagcc atgagtttgt caaacttctc aaggtaacgt
    23761 ggtttattac ggaaaggaag agcacaagtg tccaaggtac agtggattcc ttgttcctta
    23821 gccttggtga agagggcaat caggaaatca atctgcaaga gagcttctcc tccactgact
    23881 gtaatcccac ccttatttcc ccagaaacca cggtagcgca aggcctctgt caagacatca
    23941 tctaccgtcc gttcacgtga cttattggac tccatagccc aagtgtctgg gttgtggcaa
    24001 tactggcaac gcatgtgaca gccctgcaaa aagacaataa agcgaatacc gggcccatct
    24061 actgacccaa agctttctgt cgaatgcacc attcctgtca cttgtccata atcaattgtt
    24121 tcttcagaca tatcgttacc ttccttgaaa acgttttata gttttattat atcacgactt
    24181 gaaggaaaaa caagattttt gcctattttt tagaaagcaa tctgtttttc aatttcattt
    24241 tcaatctatt atcagattat tcttcaaaat cgaagccata taaggttgca aaatagtcct
    24301 cagggcgctc tgcacggcgg atttgacggg ctttaccttc ttcggtatag aggatttccg
    24361 cagaacgtaa tttggcatta tactggtagc ccattgaaaa tccgtgggca cctgtatcat
    24421 gaattaccag caaatcaccg atttctgtat gaggcagttc gcgattgact gcaaatttat
    24481 cattgttttc acagagtgaa ccaaccacat ctaccgcttc agctggtcca tctggatggg
    24541 tcacgttgct aatatgatgg taagctccgt acatagctgg acgcatgagg ttgactgctg
    24601 aggcatctac acctagatag gtacggtagg tttccttctt atgagtgact cttgtgacta
    24661 gagcaccgtg aggtgccagc ataaaacgac ccaattcggt gaaaatcttg acctgaccaa
    24721 gacctgctga cgtaagaact tcttcataca ccttacgaac tccctcacca atcaaggcga
    24781 tatcgttcgg ctcctggtct ggacgataat taacaccaat accgccagaa agattgataa
    24841 agtctaacga aatgcccaac ttttccttga tttcaacagc cagttcaaag agctgacgag
    24901 ccaactctgg ataatagaga tgggtcacgg tattggacgc taggaaggag tgaagcccaa
    24961 aagtcttggc tcctttttcc ttcaagatag caaaggcttc aaagagctgg tccttggtca
    25021 tgccaaactt agcctcccca ggattgtcca taatgtctgt ccccagttca aaaacgcctc
    25081 caggattata acgacaagag atgatttctg gaatgcctgc tactctctcc agatgttcaa
    25141 tatcttcaaa ggcatccaag ttaatggtcg cacccaattc acgtgcatag gcgtattcct
    25201 tgtctggcgt gttgttggaa gagaacataa tctcagaacc cagaaagtcc agtttatggc
    25261 tcatcaaaag ctctacataa ctagagcagt ccacaccaca accttcttct tggagaattt
    25321 tcaaaatagc tggagttgga gtagccttaa ctgcaaaata ttccttaaag cccttgttcc
    25381 acgaaaaagc ttggttgaca gctcttgcct tctcacgaat ccccttctca tcatacaagt
    25441 gaaagggagt cgggaactcg gcaacaatcg cttctaactc ttctctattg ataaatggtg
    25501 ttttcataag cttccttcta ttctgtttgc aaagaaaggc tgggacaatc gttccaacct
    25561 ttagttctat ctcttattta tcttcgtcaa agatattgcc aacctcaaca tcgatggctt
    25621 ggttcttgac atcaatatta gttaccttca agagtgactt gtagtcaaac tgcttttcac
    25681 gttcttcttg ggcattgtcc gcaaagaccg ctacacctgc cagttctgag tcgaactcgc
    25741 gcaagagact aatcataccg ttgaccgttc cgccaccttt caagaagtca tccacaatca
    25801 agacacggct gcctgcctta agactacgtt ttgaaaggaa cattttctcg atacggtcac
    25861 cacttgaacc tgaaacatag ttgacgctaa cagttgaacc ttcggtaatt ttcaggtcac
    25921 ggcgcacaat gacaaaagag acattgagga cattggcaac tgcatttgca agtggcacac
    25981 ccttagttgc tacggtcata accgcgtcaa ttttttggtc cataaagctt ttggcaataa
    26041 tacgaccaat atttttcaag atggctggtg tgctaagcaa atcagacaga tagatataac
    26101 cacctggcaa gatacggtca ctttctgaca acttggtacg caagtcttca accatttcct
    26161 tagcatcctg actcgaaata gacggtgtga agatgacacc tccgccagcc ccagtcactg
    26221 tctggatatg accgatttca atttcctcaa aggcgcgttt gataatgacg atatcttctg
    26281 agatggatga tttagcagac tcatactttt cagcaaaagt attgagacta gttagtttat
    26341 aaggattatt aatcaaatag ttggaaatga caaccatccg atcacttctt cttaatttca
    26401 tttctatttc ctattccatt tttattcaaa aaatcaaaaa gcaaactaga aagctggtcg
    26461 ctggtggttc aaaacactgt tttgagattg tcaatagaac tgacaaaccc tgtaatatac
    26521 ctgcatatat acatacgaca aagcgatact accctagttt gaagagattt tcgaagagta
    26581 ttcatttttg tcttttactt attataccat attcacataa aaaaacgaac attcttatcc
    26641 taaaaaatgc tcatttttct taaattatca atctaaatct ggtttataga aggaacgatt
    26701 atccatagcg aagattttat tggacatctc tcctttatcc accaaagcca gagctgttga
    26761 catcatcatc atgcttgcat ccagattgtc aatcatatgg ataatctctg cctccataat
    26821 acgtggacgg actggacttc catattcaag caagccgtgg tgactgagga tgacatgacg
    26881 aagcaaaacg acttcttcct tggtatcatc gatgccgagt tccataactg tcttggtaat
    26941 ttcgctatca atgagagcga tatgtccaag aagattacct cgcactgtgt actctgtctg
    27001 gtctggcccc gtcaactcga taaccttagc taagtcatgc agcataatcc ccgcatagag
    27061 taagctctta ttgagctgag gataaacttc gctaatagcg tctgccaaac gtaccatggt
    27121 cgccgtatga taagccaagc ccgtttcaaa ggcatggtgg ttggtcttgg cagctggata
    27181 ggagtagaat tccttatcat acttggtgta gagatttcgg acaatccgtt gccagacagg
    27241 attttcaatt ttgaaaatca tttgcgacat gtagtcacga atttccttga catcaactgg
    27301 tgacttgacc ttgaaatcgg ctgggtcatt gggttcacca gcttgaggca ggcggagagt
    27361 aatttgattg acttgagggg tattgttata aacttctcgg cgtcctttca tgtggacaac
    27421 cttacctgcg gtaaaggcct caatgttatg aggttgggca tcccagagct tcccatcaat
    27481 ctcgccacta tcatcttgga aggtaaaggc taggtagttt ttcccagctc gagtttgcct
    27541 caggtcagct gatttgatta ggtaaaagcc ttcaaatagc tcatcttttt tcatgtgact
    27601 aatcttcata ttcttcctca ttttcttgaa aatggagtag atcaagcgca ggctcgcctt
    27661 ctgacaactc aatgtgacgg agcgtccgct cgatagctat ggtacgacgg tttaataatt
    27721 catcaatatt gccagaggca tgttggagat gtttttgtgc cttgaccaga ataccaccaa
    27781 acttgccaaa ctcggtcttg acactggcaa gagtcttgct gatatggtcg gcactctttt
    27841 ggatattaag ggtcttgaaa ccaactgata gggagttaag aagggctgat agggtacttg
    27901 gtcctgcaac gataatctgt tcttcccgtc tcaaatcatc aaagaagacc ggattgcgga
    27961 cgatttctga gtagagacct tctgtcggaa caaacaaaac tccaaaattg gtcgtccgag
    28021 gtggtgctat gtacttgttc ctaatatccc tagcaaaacg cttgacactt gctaggagtg
    28081 acttacgaca gcgttcgatt tcatccttgt cacctacttc ataggcttct tctaagcggt
    28141 aataatctgc cagtggaaac ttagagtcaa ttggtaggta gacgtattct tggtcgcctt
    28201 gtccgggtaa cttgatggca tactccactc gttcactaga gttttcaacc gttgcgtatt
    28261 ctcgttcgta ctgggcaggt gtcatgatgt cttcaataat ttgccccagt tgcaattctc
    28321 ccagaatccc tcgcgtcttg gttccagaga gaaccttgtt aagagctccg acatcacggg
    28381 caactgtctg catttctcca agaccacgat tgacagactc cagttgttta gaaactgtct
    28441 caaaggaagc ctgtaagcgt gtctgcaagg tcttttctag tttttcctcg accgtctggc
    28501 gcatttgttc caaacgttgc tcatttgatt cctgcaaggc ttggagacgt tggtctgtct
    28561 tgtctctagt ttggaggaga ttatctgtca tttcttgacg gacttgggtc aggccttggt
    28621 gcaattcaat ccgcacttct tgcaaacggt cgctgacagc cacttccaaa tctttttggt
    28681 ctaactggct ggcttgtctg gcttggtcaa agcggtaatc caactggtct gacaaatgat
    28741 ctgcctgatc ctccaagctc ttacttaagt gtttctcctg cctatcctgt ctttgccaaa
    28801 tcagaaagag accagctagg ttggcaatta ataatagaat aagtaaactt tccatcctac
    28861 ctcctgtcct tgctatgcag tacgaccaca taaccatctg ggcaagtcac cgacacttcc
    28921 ctatctatat attcgttaga agcgtacact tttttaaaga aaaaattctc ctctgtcaac
    28981 tcatacttag ctccaagaat agtcagctgg ctatcccgaa ctggcataaa ggctagatag
    29041 tcgtagtctg aacgaggttc tagctgactg gtcccttctg aacaataggc aatcaaattt
    29101 tgcccatcct caatttctat ttgacgcata tagggtgcca acttaggatt gctaggtaga
    29161 aagacattgg ccaacatatg gtcaatacgg ccacccaagg caccgaaaat agtgacctca
    29221 gcctgaggat tttgttcaaa gatggttaag agagccaatt ccagatctgt atcatccttt
    29281 tctggtcggg cttggacaaa atgctgggca cgtttttgaa tcacctgtcg ctcttcttcc
    29341 gtcacagaat caaaatctcc gactgctaga gcaagaggta agtcttcttc caagacccag
    29401 agcgagcctc gatccacccc aacaaaagca tcaaaatctg tccgataatg accgcggttt
    29461 ccgcctgcaa aaacggcaac cctagtccag ttgttttctg agagtttgta ctcgctcatt
    29521 gacttctccc ttaaagacat aggaacctgc tacaaaaaca gtcgcaccag cttctttggc
    29581 ttgagcaata gtttggtcat caatcccacc atccacttcg atttcaaagt tcaaaccttt
    29641 ttcctcacga agagcaacca actcacgaac cttatccatg gtttctggca gaaaggcttg
    29701 cccaccaaaa cctggattaa ccgtcatgac taaaacttgg tcaactagat gaaggacgtg
    29761 cttgatggct tcaactggtg tgccaggatt gataacgact gaaggcttaa ctccgagtaa
    29821 acgaattttt tggagggcgc catgaatatg aggtgttgct tctacatgga tactgatgat
    29881 gtctgcacct gcacgcgcaa aatcttccag atgatgctca gggtttgaca ccatcaagtg
    29941 gcaatcgaaa accatcttac tatgaggacg aaggctctcg accacacctg caccaaaact
    30001 gatttgcggt acaaaatgac tgtccatgat atcgatatgg gcatattctg ccccagttgc
    30061 ttctagacgt ttgatttcac gttcaaagtt ggcataatct gctgccagaa ttgacggagc
    30121 aatcttgtat tgagacatag gtttctcctt attttggaat ttttttgctg acttttttat
    30181 aggtttctct acgattttca atttcactaa ggaattgcag gtaattatca aaacggaagg
    30241 ttgcaataac accctcttca acagctggtt tgacggcaca agacggctca tgggtatggg
    30301 tacaagtacg gaacttacaa tctcggctaa cagtagcaat ctctgggaaa gcctgattga
    30361 ggtcttcagc ccttgatact tcatagtcca gagatgaaaa gcctggtgta tctgcgattt
    30421 tacccccgtt gagattgtaa aaactaacag ctcgagtggt atggcgaccg cgacctagac
    30481 tgtctgaaat ttctcccgtt tcaagattga ggtctggtac gagtttattg agaagagttg
    30541 acttcccaac acctgtctgc cccataaaga ccgtaacctt gcctgttaac aaagacagga
    30601 gttcctcttt actggtcaca aagtcatagc cgatgtcacc ataggtctgc cggtaaaaat
    30661 ccagttctcc cctatcttcc aacaaatcca ttttggaaat atagacaatg ggatggatgc
    30721 ccttgtgctc caaaagaacc aagaaacgat ccagcaaatt gctgttaaaa tcaggttcct
    30781 tgacggacat gattactaca gcttgatcga tattgacaat aggcggacgg accagactgt
    30841 ttttccgttc gtgaattttg aggatatagc cttctgaatt ttcctcggca gagaaatcta
    30901 cccagtcccc aacataaggg gtatggcctt ttttacggaa attcccacgc gcgcgtgttt
    30961 gataaacctg gccatcactc tccacatagt agaaacctgc caaggcttta atgatttgtc
    31021 cctgcatctt agagtccttg ccctttaagt gcgtctgcta gaccggcaaa ttctgccaag
    31081 ctgagagctt ccccacgcac acttggtgac aagcctgcct ggtccaaagc cttggtcagc
    31141 ttgtccttga cctcttcagt cttaccaaag taacctgtca agttattcca caaggtcttg
    31201 cggcgatggg taaaactagc cttggaaacc ttaaagaaaa agttctcatc ttctactgct
    31261 acggctggct ctggacgacg caccattttc aagatggctg aatccacatt tggcgctggc
    31321 acaaagaccg tacgaggcac gataaaggca accttggctg tcatgtaata ctgcacggcg
    31381 atagacaagc taccgtaagc cttggtgtta ggctgggctg aaatgcggtc cgctacttct
    31441 ttctgcatca tgaccacaaa ctcacaaaaa ggaatgccac tctcaatcaa gtgcatgaga
    31501 ataggcgtcg tgatgtagta aggcaaatta gccactacct tgattggcag gttaggattt
    31561 ttaaaatttt ggatatgttg cgccaaatca accttgagaa tatcttcgtt aactacagtc
    31621 acattatcaa aatcacgcag ggtatctacc aaaattggca ccaaacggtg gtcaatctca
    31681 aaagccatga cttgggctgc acgctcagcc agaaactccg tcaaggcacc aatacctggc
    31741 ccgatttcga tgacattgac ctggtcatca atttcagccg tatccacaat tttttgaagg
    31801 atattggtat ccgtcaggaa attttgcccg aaggactttt taaaggtaaa accgtgacgc
    31861 tccagcactg ccttggtcac gctataatct gcaattctca tctattctct tttctattat
    31921 ctgttactac tattgtaaca cattcccctt gtattttggg aatgaaaatc ataccaaaaa
    31981 actatctttc ctcatgaaga aagatagtct tggaaatctt taaaagtggc cttaacagtt
    32041 ctctcgaaac atgcgttcaa aaaaatcagc ttcttgataa cttcgatttt caggtagggt
    32101 caaatccaca tcctcctcca catcatccac tgtcagttca accgtctgag gtaaattttt
    32161 caaacgataa tgcttttgcc tttgcccttc aatactagca aaatcaagca aatagccatg
    32221 cactcggatt tgaaccaaaa ggagatgatt ggtcatattt tccatgatga ggatatcttc
    32281 taaaccacct ctggcactag gataatcaga taccctgcga atacggattt tttcacacat
    32341 gagcaagttt cctcatatca atgatttcat cagccttatt ccagactagg ggattatggg
    32401 tcgcaatgat gataattcga ttttcatctt tcaaatccac caagagattc ataacctcct
    32461 ctgaattttc aggatcaaga gctgctgttg gttcatctgc caaaatcaag ggtggatttt
    32521 tcaaaatagt cttagcaagg gcaactcgtt gggcctctcc cccagataaa gtatagattt
    32581 tttgttctaa atccaaatac cctagattaa ctttttctaa agcccccact tgcctttcca
    32641 aacgttctac ttttgagatt ttctgaccaa caaaacccaa atccaaattt tctttgattg
    32701 attggttttc taagaggccg aaattttgaa agagatagcc catctggtct cgaaaatact
    32761 cacgagtggg aatggttttt aaatctttcc cctgatagag aacccttcca ccatctatct
    32821 tttctagctt tcccaagata ttcagcagcg tcgtctttcc gcttccactc tttccgatta
    32881 aggcataaac cttgcccttc tccagcttga gattcaaacc agagaaaatc gcgcggtcat
    32941 taaatttctt ttccaatcct tgaatatcaa tcatcttatt ttcctttcaa tactgccatg
    33001 ctaccagctt cttctttttt gtcctgcctt actaaaatca agagggcgtt aagcgtaaac
    33061 aaagctacaa ctagagcgct aatcaaacca tctcttgtca aaatactaga taggactaag
    33121 ccaagcaaga gaactcctcc ttgcgccagt aaatacttgc catgaagctc atagattgtc
    33181 ataccagcaa gacgtttaat cataagctcc cgtctgaact gctcaaaata gagaagattc
    33241 atggaatcaa ataacaagat agccgtagac agggtcaaaa tcgtcccaat aaggagagag
    33301 taaaactcca cccgtttgtc atttagtacc ttggcaaaaa atagctggct ttttaccaag
    33361 taagaaacct tgtgatacag accttgctct tttagagctg tgatgctgtc tccatatcga
    33421 tctagtttca aggcactatt agccgtagtt ccccacaaca tccctgcaac aggtcttgtt
    33481 ccagtcgctt gcggcgttag aactacaata atcggatctt ttaggtactg gcgtgttgtc
    33541 ttgtacccat cattatagag gaaacgttcc tgtcctgttt ctgtaaaagc tagccttacc
    33601 tgtgggaggt agtgtttctg actcgtcact tctacacttt cagatgaaaa attttgcagg
    33661 taatctgtaa acaatccttg gtagtagaca gactgctctc gcaagctctc aggcaagatc
    33721 agcccaaact ctccctcaga caagttttgc atcttgtcca taaactctga agaaacggta
    33781 atcttttctt ctatcagata gcgtggtgag acatagataa cattccctga cggtgtgtag
    33841 tcactgagac gattgccatc taggtccact tctgctccat ctgagaaatt gtcaacattg
    33901 ctcataatat aaaaagagtc tgtattggct aaccgttctt cagtaaatgt ctgccactca
    33961 cgattatcct tacgcgttcc ttcttcatcg gcaaatgcac tagaccaacc aaaggataga
    34021 cggtaacggt ctgaggactg gctccattta ttgctagctc tctccatttc ctgcatttca
    34081 cggtagtggg gtaggagagc tgtcgcactc gatccgacca ccaatacagc taagagttgc
    34141 cccaccatca tcaatgtcat catacgtttg agagggagtt tccctttcaa tagatccacc
    34201 agactatttt cctgtaaacc aagtagatgg acgacactta gtaaggtaga aatccctgcc
    34261 aaggtcaatc catagagtag aagagcaatg atgaccagtt gcaccgttgc cataaacaag
    34321 gcaccttgat accagagaat ccccaatccc aaaagactgg ataccagcac tgagcagata
    34381 agctgtctca catcttctaa cactggtctg agagcaactc caaacaagct ctcaccagct
    34441 attaagcgaa tccctgcttg acgaagggat tttatccgat aaatcagggt cagactcata
    34501 aaggtcagca gaaaaatagc taaactcagt agcaccatag gggtggccgt caatagtaag
    34561 actatcgaaa atggatcttc tccattcgaa acaaagcctt gatagccaag ttctttcaag
    34621 gtggtctgta agctcactcc atccaaactt cctgacacaa tcaagtaaga ccctaataaa
    34681 tcactcgttt ctgcactctc cttggaggaa atggtcagac cttctggaag ctctccctca
    34741 ccataagttg cataggtaaa gcgtgtttct ccagcttcat ttggctcaac gattcgacgg
    34801 gcgatgagac tattatgctc taccgccaag tgactcaaag cctctgtcac ctcttcgcga
    34861 ctagctactg gttgatttgt catcattcct gtcacatcaa cagaaggata gttaccaaat
    34921 aaaatctctt ctcgatggtc aacggaagtt atcaccatat aaagcgatac caataccatt
    34981 gaaatcaaaa taaataaacg tttcatcatt tcctccaaaa agttacttgg tcacttagtg
    35041 agaaggtgac caagtaaagc taaacaatag ggctatacca ttctaaacat tatagtagaa
    35101 tgcaactttc tctccccaag aagtacgaat ccatgcacga gaagtccctc cagcaccagc
    35161 atatcccttg tcagatgaac cagtttttct gctaacaact gttgaactat ggtattttga
    35221 agggtgatag tagtttgagt aagcaccacc gttacctgaa ccataagtcc aaacaccacc
    35281 ttctgggtat tgaacggctg cagaggcaac tgctacactt ccacctaatg ttgctgcaat
    35341 gctcgctaca agagctaatt ttttaactgt ttgtttcata ttgatactcc tctatttttg
    35401 attacaagat tgttgagtta cttgctcctc tttccattat caacgttgat actattatat
    35461 taccaaatga tattctaaaa ttatttgttt ttctctaaat tgtcaaaata gcaccctgaa
    35521 atgaactttt atagtcatta tactccatct ttatcacact atcaaggaca tcaatgtcct
    35581 taaaatctaa ctatttcata ttatttttag cctatatcta aaaaaacagt tctaaagacg
    35641 aactttagaa ctgttttgat acgaggacaa ggacattttt gtcactgatt cactatgttt
    35701 ataaatattt tttgacatcc tcttgccatt cctctgtcaa tttctttact agaaattcat
    35761 ctccaattag ctgcgcaaat gttttagaag actgataaaa tgattcagct aaactaaaat
    35821 ctctttcttc tcttaatgct tgcttccaca aaaacataaa tacaagaggt ttcttttgat
    35881 aatcttgagt tttatccata atttcttgta aacacgatag ataatcatta aaacaatcag
    35941 ttactcctac cttttcaaga caacacattc ctgaaataag agtatctcta attataaaga
    36001 agttttctaa tttagttacc tttttctgtt ccaaaagaaa gagcattagc ttttcgattt
    36061 gatttataaa tttggcaaga tcttttctaa tgagcatttg ataaaaaaac aaacgaatca
    36121 acaacatatc atttgttgaa taaacttctt tttctatgat ctgagacata tgttcctcaa
    36181 gtattaactc tgcatattca ggccttctag ttctagaagt atccaaacac gcctgcaata
    36241 cttcacatgc taattgttct tctttaggaa gattatcaaa gtatgtttta taaatctctt
    36301 ccaaacacgc ttccttctta tcgtactcct cttctttacc atagattggt tcacgtaaaa
    36361 tctggtattt caattctaaa taagcaggag gaagagctga aaaatccggc ataaggctat
    36421 aaactggaac tcctagccgg cgagcaatat aggctaactt gggcaaactc ggttgcgaag
    36481 ctccactttc aattcgcgac agttgacgaa ctgtcagttc ttgctcatct ccacaaaaat
    36541 ctggacgact aactcctttt tctatccgaa tctctttaat tttttgacca attaacatca
    36601 gacgcctcct cttctacctg cttcgaaaaa tactgtgaaa cttaaaaaat tgtaacggtc
    36661 acatctttcc tactcataag atttcatagc ttcttccact tctgccaaag taaccccaaa
    36721 caactctagg cgtttgagga gttgcttgcc gttagaatag ccgattcgga gagtctctcc
    36781 gagatattcc ctacgcttac ggctgtctgc ccctactaga aaaccaaggc gaatcaagtc
    36841 gctacgacta atgtcaaact gactctcatg ttcaaattgt tctgtcacct gagctagagc
    36901 cgttttcagg tcttcatagc tggcatgctc aattcccaga gaacgcccct tggtcttgga
    36961 cttgggaaca gcttcatctc gcttgagaaa ggcatgctga actgttggaa tgaccgtcat
    37021 gatcatgcgc cgaatccgtt ccccattaaa atctgggtct gtaaagacaa tgactccatg
    37081 acgttggtgc aggcgctgaa tccgctctat atcctgatca ttgatggcag aacctcgagt
    37141 ctcataggtc tccacatcga aataacgttt gagattgacc gtatcatcac gcccttcaac
    37201 cacaattact tgggaaattt tctctttcat tacttgctgt ccagtccaaa aattcgttct
    37261 gcatttgcag tcgttgctac cgccagctct tctgtcgtca taccacgcaa gtcagcgata
    37321 aagtcgacca catagcgagt ataggctgtt ttattttcac gaccacgctt gggtacaggt
    37381 gctaagtaag gcgcatctgt ttccaccaac atcttgtcca aaggtaactc tttagctgct
    37441 tcttggaggt cagttgcctt cttaaaagtc accactcctg agaaggaaat ggtcatacca
    37501 agatccacaa acttctctgc ccactcaagc gtccctgaaa atgaatgcat gataccacca
    37561 cgaggaccaa cgccctcact cttgataatc tcataggtat cttccagcgc atcacgggta
    37621 tggacaacaa aaggcaaatc caagtcctta gatagctgaa tctgacggcg aaaaacctgc
    37681 tcctgcacct ctttgggcgc tgtcatccaa tggtaatcta agccaatttc acctaaagcc
    37741 acaaccttgg gatgttttaa cttatccaac aagtaagcct caacttcctc tgtataagta
    37801 ccagcttctg taggatgcca accaatagtc gcatagagct gctcatactc atctaccaac
    37861 tccaaggcac gctcaatcgt cggtttatca aaaccaacaa tattcatctg tgtcacaccc
    37921 atctcagcag ccaaggcaat ttcttctgcc tcacgacctg caaattcttc tacattcaag
    37981 tgtgtatgtg tatcaaaaat catctcttct aacctcgttt tctatcttct attataccaa
    38041 aaaagaggag gggcacctaa tttttcggtt tcccctcctc tcttcaatag agagctattc
    38101 tgctatcttt tctatccgat attgcccatc tcctattcca cagttagaga cagaagagat
    38161 tggctacatt gatagactat tcctattcag ctaaagtatg tgattatctt gggttctcac
    38221 ttttctcacc catcactagg attgatgact tctcaaaatc ccccttataa tctaacttta
    38281 agtagagaat agaattttct agctgttcca ttttctacgt ctagtctttc ttcctagcaa
    38341 agcaaaacca ctggttgccg ccactagacc aagactagca agtgctgcac ttgatgctga
    38401 acctgtgtta ggtaattgtt tgccagcagg tgactggtag gtaacatctt gcattgttcc
    38461 tgcagttgct tgcggtgcac ttgttgtctt aggagcagca ttaggagcca ctggtctctt
    38521 aagaactgta accatggcaa tggcttgcac tccaatcgtt tctgcttcct tcacatcttc
    38581 catagttttc gtattttcaa tctctttgag agctgcttgt ttttctgctt ccactcttgc
    38641 taaaagttct gctttttctt tatcagaaag cggtgcgcct ttgatttcat cctgcttgtc
    38701 cttggctgct ttttcaatgg catttttagc tgattctttt tcagtagcta attgcttaga
    38761 agcttgtgat tgagctgagt tttgtgaacc tgtgtgagct ggtccacctg cctggctacc
    38821 accgcctgca ttcgcattag catctgagct tccagtattg cctcccttat ctacacctag
    38881 tgtagtttct ggcttatact ctggtgtagc ttcttgagtt acagattcac ctttcgcaga
    38941 ttgttgaact gtatcttttc ctagaatcgt cactactgaa ccatctggga atgtgattgt
    39001 tgctgtacca tctccagcta cttcaacttt atagcaaaat gaaacaataa caggacaaat
    39061 cgatcaggac agtcaaatcg atttctaaca atgttttaga aacagaggtg tactattcta
    39121 gtttcaatct actataacat cgcttccgaa tccatttgca tctactgaac ttttttacaa
    39181 aaataaaaat cagtagactc gagttcctac tgatttttga tagaattcgt ttggattatg
    39241 cagccaaaac tttgcgtcct ttacgacgac gagctgccaa tacgcgacga ccatttttag
    39301 ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg aagtttactt ggttgataag
    39361 tacgtttcac gatgaatacc tcctcataga ttttgtattc gtttagccgg ctataaagtg
    39421 atcagttact aaacatactt tactattcta tctgattctt tcacttttgt caatagctct
    39481 aggcaaatgt ttccagcttt ttcgaatgca agccctatct acgatactgt ttcaagaaac
    39541 gagtcatctt ttcttggatt tgggctaact catcaacacg aggaaggtaa acgatacgga
    39601 agtggtctgg ttcctgccag ttaaagcctc gaccatgaac caagagaacc ttttcctgct
    39661 tcaagaaatc aaggacaaac tgctcatcat catcgatacg gtacatattg cggtcgattt
    39721 ttgggaagat atagagcccc gccttgggtt taacggcaga caaacctgga atatcttgaa
    39781 tggcattata gatgaaattt ctttgctcgt agattcgtcc accaggaaga agcaattcat
    39841 cgactgattg gtgacccccc aaggaagttt gtacgacttg ttgggccaaa acgttagagc
    39901 aaaggcgcat attggacagc atattgagcc cttcgatata gcccttaaca tgagtcttag
    39961 ggccagacaa gaccatccaa cccacacgga aacctgctat gcggtgggat tttgacagac
    40021 cattcatgct gacacagaag acatctggtg ccaagctcgc cacaggcgta tgcacatgtc
    40081 cgtccattac catgcggtca tagatttcat ccgcaaagat aatcaaatcg ttttgacggg
    40141 caatctcaat aatctccagc aagagttcct taggataaag ggctccagtt gggttatttg
    40201 gattgataag gacaattgcc ttggtattgg aagtgatttt tgacttgata tcgtcaatat
    40261 ctgggtacca ttccacagct tcatcacaga tatagtgaac ggcatttcct ccagctaggc
    40321 tgacagccgc cgtccataga ggatagtctg gcattggcac taagacctca tccccattgt
    40381 ccaaaagccc ctgcatggac atgacaatca actcactgac accatttcca aggtaaatat
    40441 catcaatatc tacgtttggg aatttcttca gctggcaata ctgcatgatg gccttacggg
    40501 ctgagaaaat ccctttggag tcagaatagc cttcactatc acgcgcattc ataatcaagt
    40561 catgaatgac ctcgtctggc gctgtaaagc caaattctgc tggatttcct gtattcagac
    40621 gtaaaatctt ttctccattc gctcgcatcc gcatggcttc ttccaataca ggaccacgga
    40681 tatcataagc aacatgctct aacttactag acttgttata ttctttcatc ttgtttcctc
    40741 aattcatcag gatagtaaca ttataccact agaaagagaa tgcgacaagt ttactgaaat
    40801 gtgttactag tttcgtatag aagaaaaacc ttgctaaagg cgaggttaag aacgtagaga
    40861 tagcaggtat tttgcaaaga cacatcacat accaaaagat aaacctagaa aaagcaagcc
    40921 tagaaaggag tcataaagaa gattaaacat gaggaaaagc ccccatatca tcaagtaatc
    40981 ccaacgccca cttcgttttg caactagaaa taagattaga tggtagagta catgaaaaac
    41041 taaaaatcca ataaaacttg gataaagaag cggtaccaga ctctctaatt gacagatcat
    41101 gatcacttct accaagactg aaactaggat aattccataa taaaggaaat ttgatttttg
    41161 aaaagagttt ttcatcattc tcccttcctt cacttctttc ctgctatcct ttcttttatt
    41221 ttatcatata tttatataga gtttaagccc taatattctt ttgaaaaata ctttttttgc
    41281 tttacttcta tagtgaaaag gtttacaatt atagtaagaa aatttcagga ggtttcatta
    41341 tggcacaacg ttaccaaaat atcatggtcg caatcgatgg ttctaaggaa gcggacttgg
    41401 cttttgtcaa gggagttcat tctgctctac gaaacgacgc taaactcacc atcgcacatg
    41461 tcattgacac acgcgctctc caaagcgtat ccacctttga tgctgaagtt tacgaagaac
    41521 tccaagtcga cgctgaaagt ctgatgaaag agtacgaaaa acgtgctaaa gatgctgggg
    41581 tagcagatgt tcatatcgtc attgaaatgg gaaatccaaa gaccctgcta gcacgtacta
    41641 ttccagatgc cgaggaagtg gacctcatcc tcgttggcgc aactggtctc aacgcctttg
    41701 aacgcctctt ggtcggctct tcatctgaat acatactccg ccatgctaag gtcgatttgc
    41761 tggttgtgag agaacaagaa aaaaccttat aatcacaaag aaaaggagcc cctagctcct
    41821 ttttgtttac gatttatttc tctctttatg gcgttcgtaa gccttgagct ggcgctgcag
    41881 ttccttttta atagcaggtt ctggagcata tttttcttcc caattatctg gttttaagat
    41941 tttatgggtc actggatcaa aatgagcctt gccatctgga aaaattttcc ccatattggc
    42001 ctgatggaca atatcaaaaa tacgttctgg gtccaccccc atcaagacaa aactgccgta
    42061 ggtgaagtaa agcgtgtcaa tcaaggcatc cacttgccct atcaaatctt gctgagcagg
    42121 tgtcttcttg gctactttat ctgctgcctt atcaagggcc tgatgaagtt gcgatacagc
    42181 ttgaccaaaa tcttcttcag aaggactggc tgctcgaaca aactccacca attcttctat
    42241 tttaaaacca gccctatggg ttgcaccctc taaatcccaa gctcgaggtt cttcttgggt
    42301 tcgttcatcc atcatgtggt ggaaggtctt gaccttattg aaatgatagt cacggctgac
    42361 aaagactttt tctgaagaca aaacaccaaa atgttccaag gccttgtgga tcccatcttg
    42421 ttgattgcta gtggtaatgt gcttggcagc ttccttgaca ctgctactac catttcccat
    42481 agcgaccgac ataccgacgc cagccagcat ttcaaggtca ttatctgagt caccaaaagc
    42541 catgacttga ttgaggtcaa agccatattc tttcccaact cggcgaatgc cttctaattt
    42601 agaatttccc tgattgatga catccgatgc aaaaggattg ctacgtgtca atttcaagtc
    42661 ttcaaaatca gctgccgcct tctcagattc ttctggtgtc atcagcatca aaacttggta
    42721 gataggctga ttcatcaggt gaagcaggtc ctcttccttt tggggaacaa ccttgctgac
    42781 catgcgatta aaagactgac tcaccgtccg agttaaaaca gagggaacga agcgactaat
    42841 tcgttgggaa aaagaaccca gaccaaagga catgatttta gaacccaaca tggcatcctt
    42901 ggtccctagg gcaatctccg tgccctcttt tttagcatag ctaattagat ggcgcaaatg
    42961 taacttggaa atagggctcg tgaacaagac tctgtcttta ctaaagatat actggccatt
    43021 ataggttacc gcaaaatcca gatccaaatc gtccatcaat tccttaacaa aaaaaggtcc
    43081 tcgccctgtc gctacgccaa ctagtacccc ttgttctttg acaatcttaa tcgcatcctt
    43141 agtggatttc aaaacactct tgcgattgtt gaccaaggtt ccatcgatat caaaaaaaac
    43201 agctttgact tccatcctat cccaatctcc ccttttgtga tacaatgatt ataccacatt
    43261 tcagaaagag ttagtaaatc atgcctaaga aaatccttgt tttacatacg ggtggaacta
    43321 tttccatgca ggccgatgct tctggcgctg ttgtgacgag ttcagataat cccatgaacc
    43381 atgtgtccaa cccacttgaa ggaatccaag tccacgcctt ggactttttt aaccttccaa
    43441 gtccccatat caaacccaaa catatgctgg tcctctacca gaaaattaaa gaggaagcag
    43501 ataactacga tggagtggtg atcacacacg gaaccgatac tttagaggaa acagcctatt
    43561 tccttgatac catggaagtt ccccatatgc ctatcgttct aacaggagcc atgcgtagct
    43621 ccaatgagct cggtagtgat ggtgtttata attacctaag tgctttacga gtggccagtg
    43681 atgacagggc tgctgacaaa ggagttttgg tcgttatgaa cgatgaaatc cacgctgcca
    43741 agtatgtcac caaaacacat acgactaatg tcaacacctt ccagactcca acacatggcc
    43801 ccctcggtct catcatgaaa caggaaatcc tctacttcaa aacagctgaa cctcgtgttc
    43861 gctttgacct tgatcacata caaggtttag tccctatcat ctcggcttat gctggtatga
    43921 cagatgagct gattgatatg ctggatttag aacacttgga cggtttgatt atccaagcct
    43981 tcggagctgg taatattccc aaagaaacgg ctcaaaaatt agaaagcctt ctgcaaaaag
    44041 gaatcccagt cgctctggta tcacgatgct ttaacggtat tgccgagcct gtttatgcct
    44101 accagggtgg gggcgtacag ttgcaaaaag caggcgtttt ctttgttaaa gaactcaacg
    44161 cccaaaaagc ccgcttgaaa ctcctcatcg ccctcaatgc cggactaaca ggacaggctt
    44221 tgaaagacta tatggaaggc taatactctt cgaaaatctc tgcaaaccac gtcacgtcgc
    44281 cttaccgtat gtatggttac tgacttcgtc agttctatct acaacctcaa aacagtgtgt
    44341 tgcgcagcct ttaaccagcc gcctagtccg ctctatggta ttcattaagt caacatctct
    44401 tgtttaagag cacaaaatca ggaaatcttc tcgattccct gattttttct atttacgttt
    44461 tcgtgttgag ctacgttctg tcaaaccatg aggtaagaga acttcacgtt cttccaactc
    44521 ttccttatgc ataatcttgg tcaacatacg catactaatg gcaccaaggt cataaagagg
    44581 ttgggcaatc gttgtcaagt ttggacgggt aaagcgtgag atttgtgaat catcactagt
    44641 aataatttca aaatcttctg gcacagaaac acccttatca gccaaaccgt tcaagactcc
    44701 tgctgccaac tcatcacctg tcacaactgc tgcagttgca tttgatgaaa tcaaacgctc
    44761 tgctaaggcg taaccatcat catagctata tttagattca aataccaaac cctcactata
    44821 agcgattcct gcttttttca aggtttcctt gtagccaact aaacgaacct taccattgat
    44881 gtcatccact agcggaccgc taacgaaagc aatacgctca ttttctttag caaggtaact
    44941 cactgcatca attgttgctt gcttatagtc aatattgaca cttggcaact ggtgctcaac
    45001 atcgacagtt cctgcgagaa caatcggagt acgtgaacgc gaaaattctg agcgaatttt
    45061 atctgtcaag tgataaccca tatagataat gccatctacc tgctttgaaa agagggtatt
    45121 gacaacagaa acttctttct cgttatcttc atcgctatta gctaggacaa tattgtactt
    45181 gtacatttct gcaatatcat caatcccctt agccaaactc gaaaaataac cattggtaat
    45241 atttggaatc acgacaccga cagtggttgt ctttttactt gcaagaccac gcgcaactgc
    45301 atttggacga taatccaagc gatcaattac ctctagcact tttttacggg tattctcttt
    45361 tacattttta ttgccattga ccacacggct gaccgtcgcc atggaaacac ctgcttcacg
    45421 agcgacatca taaatggtta ctgtatcatc tgcattcatt ccttttcctg tcctttctat
    45481 ctcacacatt cttttacaag tagaggtact gattgaagct ctatatctac ttacaaaagt
    45541 gaagatgtga aaatttcgtt ttcatatttc tacttattcc attctatcac taattgtaaa
    45601 cactttcaag tgttttttga agattgattg aaaaaatttc atagaaaacc taggtttagc
    45661 tccttgctac caccttagac taaacaaaaa ggaggaaact aagccctcct aaagttatag
    45721 taaaatgaaa taagaacagg ataaatcgat caggacagtc aaatcgattt ctaacaatgt
    45781 tttagaagta gaggtgtact attctagttt caatctacta taggtattgt tccattcact
    45841 accgtcaatt ttagcacata gtcttcatga aaatattata tcatcataac caaccagatt
    45901 cttttgcgat attagctgcc tctgttcgat taccagcatc caattttgaa agaatattgg
    45961 tgacatagtt tcgaatagtt ccgtttgaga ggtaaagctg atctgcgatt tcttggttgg
    46021 ataagccccg ggcgatcccc tttaagacag caatctcttg ttctgtcaac ggattgggac
    46081 gggtcatcac cacttccatc aattcaggcg aatactcctt gcgtccttcg aggacggtgt
    46141 gcaaggtttg catgaggtct gcaacgcttc gttcctttaa tacataagca tccactccag
    46201 ccttgaccgc acgttcaaaa tacccagcac gcttgaaggt cgtcaccaca accacctttg
    46261 tttcaagcct ttctgatcgt atccactcca agacttcaag acctgtctta acaggcattt
    46321 ctacgtcaag gatggcgata tctacagact ccttttctaa tagttggatt gcttcttgcc
    46381 cattcttggc ttgaaggaca gactccacat ctggttgaag cgtgagcaat tgacacatgg
    46441 catctcgcaa catactttga tcttctgcga ctaatacttt catcttagtt tctctcctta
    46501 taaggtagtc gaacttgcac ttccgttgga tgtttctgac tgattacact tacttctcct
    46561 gaaaatggaa aaacacgatc tcggattgta tggagctcat ctccttttag aaaagcaaag
    46621 ccgcagccat catcacttac tgttaaaatg agttccttct ctgtccgttc taattttaag
    46681 taagctttag acgctttggc atgtttgatg atattggtca ccaactcaag caaaatcata
    46741 gaagccattg actccaattc ttgagtaagg ctagcagtat ctagttggtt atccgtttcc
    46801 acctcaattc cagcaatttc taacatcttt ttcacagtct ctagttcgga tgtcaaagtt
    46861 ctagacttaa gattttccac aatggttcga acttcacgca ttgattcttt gctaatttgc
    46921 tgtatttctc ttaattcctt ttccacttgt ggataagcct gcatttgaaa tagctgcaag
    46981 gctagatctg tcttgacact aagcatagca aaggtatgtc ccagactatc atgcaaatcc
    47041 tgaccgatac gattgcgctc attttctgct agcaatagat ttatctgggt attttgcttg
    47101 acctgagctt ctttcaaatc ctcgacaagg cgaattcgaa ccgagcccaa agtcatcaaa
    47161 tcgacaaaag taagaattac aagcatatag actagaaact caacttcgat tctctgaaaa
    47221 atcaaaagtc gccccacaac aaggacttga gcaaggagaa atgtccatac atgtaaagac
    47281 tttaaactac gtacgccaaa atgataaatt aagagattgg aaaggaaaaa gaaaaaccag
    47341 atataattcc cagcaacaaa ggcggtattc ccagctacat agaccagcat gatcccccag
    47401 aaaagccatg atatgcgctg gttcttagtt attaaaacac ccaaatacgt cactacaaat
    47461 agaatatcaa ccaataaatg ccaagcagga agctccccag tcactacagg taggatgggg
    47521 aaaatcataa aaattaaact tgcccaaaac atatagtgta tgcttttcag tctttcaagc
    47581 attaagcatt ctccttatga ccttgaaggt aaatggtcaa accaaacaaa actgctgaaa
    47641 aaacaagtag ataaactgtg gctgatagat tgatgccacc ctcgtttaag aaggtcttga
    47701 gcaactccat caattgatag gtcgggagac acttaccaac tgcttgcatc cagtctggaa
    47761 ataaagaaat gggcatccag agtccgccta aaacagccaa tcctagataa agaagattgc
    47821 ccacaacaga catcagctga ctagttggta agagagtcaa ggtcaagcca agtgctacaa
    47881 aagcaatact ccccacaatc agcaaaatcg cagctccaac ccattttact agaggcatgt
    47941 ctacacctct tacaaaatgt ccaactgtaa aaacaaccag aattgagacc aaataatcaa
    48001 ccagcatact tgttatcttt gatagataat attctaccat atttacaggg ctatgacgca
    48061 atgttttctg ccagttgttc atcttatcgg tatgtaaaac agctggaaat gagaggatag
    48121 ttgtcgacat catggaaaag gcagtcatgg agatgaggta atcacgcata aaattcgcga
    48181 gttcacctgg tgtgtcctga tagataccag aaaaaaataa atagaaagcc gtcggcatcc
    48241 ctaccgacaa tagataatag attaattgtc gtttggtcaa taaaaattct atcttactaa
    48301 gtgctatcca tcgtttcatc ttagttatct cccttctgcg tctcttcaaa gattgtattc
    48361 aacaaactac gattattaac ttcgatttct tgcatcctac atcctgcttg ggctaatagt
    48421 tcccagaaag catctgcttc tcgagtgact acttgtaaag aatcttgctt tagggtccag
    48481 ttttcaacca aatttgactg ctctacgact tccttgtaag ctataggaag gataaagtgc
    48541 ttttctattt cctcgctacg catagctaga ggcgtcgtat ctcgaatcaa ctctccctta
    48601 tttaagagca aaatgcggtc tgcagtgtgt tccacttcct caatataatg ggacgaatag
    48661 agaatggtga ctccttgcgc ttttaactcc tggacaattt cccaaaaacg ttgacgagtt
    48721 gaggtatcca tggacgcagt aggctcatct aaaaaaacaa tctttggtcg cccaattaag
    48781 gtcaagacga aagaaaagag acgcttttga ccgcctgata atttttctgc caactgttct
    48841 ttttgttgct tgacaaactg caatagttga tcaatttcct gattgctcaa aggatttgga
    48901 tagatacgtt gaaagaaagc aatcaactct ttaactttta atttctgaac gattacattt
    48961 tcttgaggga ggtaagccct agtatagtct aacttagaac tcgtcactgg taaaccttga
    49021 atagatactt gaccgcttgt gaccagttta tctccaagca aacaatctaa gagtgttgtc
    49081 tttccggcac catttggacc aattaaggca acacattcac catcgttgat ttcaaaggag
    49141 atattccgca aaatttcctt gtcttttatt ttcttactca atttctcaac tttaaccacc
    49201 gtcatgagac tctcctttca accactccat tcccataagg aaaacgacga aaatcataaa
    49261 tccaaacccc aaagcaccac gaatgaattg gcgaagcaag gcttgatcaa accaatcagt
    49321 aaacatttcc actaaccata ccaagagtga caggccgata aagaaataga tgatccctct
    49381 cttcattcct caaactcctt tttcacatct ctgactaatt tcaaaccttc tctaacaagc
    49441 caagacatga ttccaaagcc agcaaagacc ttccaaggaa aatggtagaa atcctcatcc
    49501 aatcccgaaa acatgagata ggtcataact cctgctacta ctaaacttac tgcgataatc
    49561 attttatttc tcatctcttc ttcccccatt tcatactaca attatagtct tttaaaatca
    49621 gaggagacag aagcttctgt cactaaaaaa tatgacaaat gtcataaaaa attctgttca
    49681 aaacaagcaa gatacactat acaataaaac acaatcagaa aaatctaagg caacttcctc
    49741 aaaagagata tcaaacccaa ttcacaccat aatgtaaact aatacttatt taaaatcaaa
    49801 aaga
//
LOCUS       DAVLTM010000005        37589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLTM010000005 DAVLTM010000000
VERSION     DAVLTM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.005"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            341..1858
                     /gene="malQ"
                     /locus_tag="VYN16_000219"
     CDS             341..1858
                     /gene="malQ"
                     /locus_tag="VYN16_000219"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1402810.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            1884..4142
                     /gene="glgP"
                     /locus_tag="VYN16_000220"
     CDS             1884..4142
                     /gene="glgP"
                     /locus_tag="VYN16_000220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1402811.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(5187..5714)
                     /locus_tag="VYN16_000221"
     CDS             complement(5187..5714)
                     /locus_tag="VYN16_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402812.1"
                     /translation="MLIVSAIPSVSAVYASEQVSQIETNMELQPVTSLTEEQINTLAN
                     EIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGITVNQMGALLDTAIALA
                     LGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALEFAMNLLSPGTYLAQL
                     WDKNDAIPNNGRINF"
     gene            complement(6648..7496)
                     /locus_tag="VYN16_000222"
     CDS             complement(6648..7496)
                     /locus_tag="VYN16_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1402813.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            7648..7926
                     /locus_tag="VYN16_000223"
     CDS             7648..7926
                     /locus_tag="VYN16_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402814.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            7928..9991
                     /locus_tag="VYN16_000224"
     CDS             7928..9991
                     /locus_tag="VYN16_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1402815.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(10069..11325)
                     /locus_tag="VYN16_000225"
     CDS             complement(10069..11325)
                     /locus_tag="VYN16_000225"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1402816.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            11467..13929
                     /locus_tag="VYN16_000226"
     CDS             11467..13929
                     /locus_tag="VYN16_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1402817.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMTKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNEHFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSRARR"
     gene            13994..14905
                     /locus_tag="VYN16_000227"
     CDS             13994..14905
                     /locus_tag="VYN16_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1402818.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            15135..15833
                     /gene="dapD"
                     /locus_tag="VYN16_000228"
     CDS             15135..15833
                     /gene="dapD"
                     /locus_tag="VYN16_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1402819.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEHDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            15901..17031
                     /locus_tag="VYN16_000229"
     CDS             15901..17031
                     /locus_tag="VYN16_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1402820.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            17043..17582
                     /locus_tag="VYN16_000230"
     CDS             17043..17582
                     /locus_tag="VYN16_000230"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1402821.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            17566..18243
                     /locus_tag="VYN16_000231"
     CDS             17566..18243
                     /locus_tag="VYN16_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1402822.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            18257..18334
                     /locus_tag="VYN16_000232"
                     /pseudo
     CDS             18257..18334
                     /locus_tag="VYN16_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(18544..19479)
                     /locus_tag="VYN16_000233"
     CDS             complement(18544..19479)
                     /locus_tag="VYN16_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402823.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDEKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAKESSSQSNSSSGASNESYNGSSNSNVDY
                     SSSEQTNGYSNNYGSQSYSGSGDSSTNGGSSEQYSSSNSNSGVNNGYYKKYKNIGADG
                     HVSYTYDNESNGDVFDENWNYLGNTGGGIAEPPQR"
     gene            complement(19897..20796)
                     /gene="galU"
                     /locus_tag="VYN16_000234"
     CDS             complement(19897..20796)
                     /gene="galU"
                     /locus_tag="VYN16_000234"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1402824.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(20818..21834)
                     /locus_tag="VYN16_000235"
     CDS             complement(20818..21834)
                     /locus_tag="VYN16_000235"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1402825.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            22046..22426
                     /locus_tag="VYN16_000236"
     CDS             22046..22426
                     /locus_tag="VYN16_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1402826.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(22600..23025)
                     /locus_tag="VYN16_000237"
     CDS             complement(22600..23025)
                     /locus_tag="VYN16_000237"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1402827.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMIGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(23093..23743)
                     /gene="phoU"
                     /locus_tag="VYN16_000238"
     CDS             complement(23093..23743)
                     /gene="phoU"
                     /locus_tag="VYN16_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1402828.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(23758..24510)
                     /gene="pstB"
                     /locus_tag="VYN16_000239"
     CDS             complement(23758..24510)
                     /gene="pstB"
                     /locus_tag="VYN16_000239"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1402829.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(24512..25327)
                     /gene="pstA"
                     /locus_tag="VYN16_000240"
     CDS             complement(24512..25327)
                     /gene="pstA"
                     /locus_tag="VYN16_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1402830.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(25320..26183)
                     /gene="pstC"
                     /locus_tag="VYN16_000241"
     CDS             complement(25320..26183)
                     /gene="pstC"
                     /locus_tag="VYN16_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1402831.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(26301..27176)
                     /locus_tag="VYN16_000242"
     CDS             complement(26301..27176)
                     /locus_tag="VYN16_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1402832.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(27343..28674)
                     /locus_tag="VYN16_000243"
     CDS             complement(27343..28674)
                     /locus_tag="VYN16_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1402833.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(28675..29382)
                     /locus_tag="VYN16_000244"
     CDS             complement(28675..29382)
                     /locus_tag="VYN16_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1402834.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(29466..29942)
                     /locus_tag="VYN16_000245"
     CDS             complement(29466..29942)
                     /locus_tag="VYN16_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1402835.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKSTIWGSTVDKDYALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTRY"
     gene            29949..30495
                     /locus_tag="VYN16_000246"
                     /pseudo
     CDS             29949..30495
                     /locus_tag="VYN16_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30893..32584)
                     /locus_tag="VYN16_000247"
     CDS             complement(30893..32584)
                     /locus_tag="VYN16_000247"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1402836.1"
                     /translation="MNTKELIASELASIIDNLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            32720..33166
                     /gene="argR"
                     /locus_tag="VYN16_000248"
     CDS             32720..33166
                     /gene="argR"
                     /locus_tag="VYN16_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1402837.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            33217..35751
                     /gene="mutS"
                     /locus_tag="VYN16_000249"
     CDS             33217..35751
                     /gene="mutS"
                     /locus_tag="VYN16_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1402838.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            35898..>37589
                     /locus_tag="VYN16_000250"
     CDS             35898..>37589
                     /locus_tag="VYN16_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402839.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT        10917 a         7154 c         8033 g        11485 t
ORIGIN      
        1 aatatatgga atctgtctaa agagggaaat caggaggggc tacctccctg tttctaatct
       61 atatctattt tctatagacg tttgtgcttt attgcatata aattcaagct ccctaaggag
      121 gttttgtact ttgtagactg tctcgtgaga gtcaaacttt aggattagat agttttaagt
      181 tatctttgct aaaatgagtt agtctcctta aaaataatca aaaaagtttt ttaaaaacgc
      241 ttgcaattat gcttgaaaag gagtatactt ataagtaacg caaacgtttg cgtctgaaaa
      301 atacgcaacg ttccattatt ttaacacacg aggtgctatt atgaaaaaac gtcaaagtgg
      361 tgtgttgatg cacatctctt ctcttccagg agcttacgga atcggatcat ttggtcaaag
      421 tgcttacgac ttcgttgatt tcttggtccg tacaaaacaa cgttactggc aaatccttcc
      481 attaggagca actagttacg gggattctcc ttaccaatct ttctcagcct tcgcaggaaa
      541 cactcatttt atcgatttag atatcttggt ggagcaaggt ttgttggaag caagtgacct
      601 tgaaggagtt gactttggta gcgatgcgtc tgaagttgac tatgctaaaa tctactatgc
      661 acgtcgtcct cttttagaaa aagcggtgaa acgtttcttt gaagtcggag atgttaaaga
      721 ttttgagaaa tttgctcaag acaaccaatc atggcttgag ctctttgctg agtatatggc
      781 tatcaaagag tattttgaca atcttgcttg gactgaatgg ccagatgcag atgctcgtgc
      841 tcgtaaagct tcagcacttg aaagctatcg tgagcaattg gcagacaagt tggtttacca
      901 ccgtgtgact caatacttct tcttccaaca atggttgaaa ttgaaagctt acgctaacga
      961 caaccacatc gaaatcgttg gggacatgcc aatctacgta gcggaagatt caagtgatat
     1021 gtgggcaaat ccacatctct tcaaaacaga tgtcaatggt aaggctactt gtatcgcagg
     1081 atgcccacca gatgagtttt ctgtaactgg tcagctttgg ggtaacccaa tctatgactg
     1141 ggaagcaatg gacaaagacg gttacaaatg gtggattgaa cgcttgcgtg aaagcttcaa
     1201 aatctacgat atcgttcgta tcgaccactt ccgtggcttc gaatcttact gggaaatccc
     1261 tgctggttcc gatacagcag cacctggtga gtgggtgaaa ggtccaggct acaagctttt
     1321 tgcagccgtt aaggaagaac ttggtgagct aaacatcatc gcagaagacc ttggcttcat
     1381 gacagatgaa gtgatcgaat tgcgtgaacg tactggcttc ccaggaatga agattcttca
     1441 atttgccttc aacccagaag acgaaagcat tgatagccca cacttggcac ctgctaactc
     1501 agttatgtac acaggaacac acgataacaa tacggttctt ggttggtacc gtaatgagat
     1561 tgatgatgcg actcgtgagt acatggctcg ttacacgaac cgtaaagaat acgaaacagt
     1621 ggtacacgct atgcttcgta cagtattttc atcagttagc tttatggcaa ttgcaactat
     1681 gcaagattta ctagaattgg atgaggcagc tcgtatgaac ttcccatcta cccttggtgg
     1741 aaactggtct tggcgtatga ctgaagatca attgacacca gctgtcgagg aaggtttgct
     1801 tgacttgaca acaatttatc gccgaattaa tgaaaatttg gtagatttaa agaaataaga
     1861 caataatcag gagacaacta aacatgttat cactacaaga atttgtacaa aatcgttaca
     1921 ataaaaccat tgcagaatgt agcaatgaag agctttacct tgctcttctt aactacagca
     1981 agcttgcaag cagccaaaaa ccagtcaaca ctggtaagaa aaaagtttac tacatctcag
     2041 ctgagttctt gattggtaaa ctcttgtcaa acaacttgat taaccttggt ctttacgacg
     2101 atgttaaaaa agaacttgca gctgcaggta aagacttgat cgaagttgaa gaagttgaat
     2161 tggaaccatc tcttggtaat ggtggtttgg gacgtttggc tgcctgcttt atcgactcaa
     2221 ttgctactct tggtttgaat ggtgacggtg ttggtcttaa ctaccacttt ggtcttttcc
     2281 aacaagttct taaaaacaac caacaagaaa caattccaaa tgcatggttg acagagcaaa
     2341 actggttggt tcgctcaagc cgtagctacc aagtaccatt tgcagacttt actttgacat
     2401 caactcttta cgatattgat gttactggtt atgaaacagc gactaaaaac cgcttgcgtt
     2461 tgtttgactt ggattcagtt gattcttcta ttattaaaga tggtatcaac tttgacaaga
     2521 cagatatcgc tcgcaacttg actctcttcc tttacccaga tgatagtgac cgtcaaggtg
     2581 aattgctccg tatcttccaa caatacttca tggtttcaaa cggtgcgcaa ttgatcatcg
     2641 acgaagcaat cgaaaaagga agcaacttgc atgaccttgc tgactacgca gttgtccaaa
     2701 tcaacgatac tcacccatca atggtgattc ctgaattgat tcgtctttta actgcacgtg
     2761 gtatcgagct tgacgaagca atctcaattg ttcgtagcat gactgcctac actaaccaca
     2821 caatccttgc tgaagcgctt gaaaaatggc ctcttgaatt cttgcaagaa gtggttcctc
     2881 acttggtacc aatcatcgaa gaattggacc gtcgtgtgaa ggcagagtac aaagatccag
     2941 ctgttcaaat catcgatgag agcggacgtg ttcacatggc tcacatggat atccactacg
     3001 gatacagtgt taacggggtt gcagcactcc atactgaaat cttgaaaaat tctgagttga
     3061 aagccttcta cgacctttac ccagaaaagt tcaacaacaa aacaaacggt atcactttcc
     3121 gtcgttggct tatgcatgct aacccaagat tgtctcacta cttagatgag attcttggag
     3181 atggttggca ccatgaagca gatgagcttg aaaaactttt gtcttatgaa gacaaagcag
     3241 ctgtcaaaga aaaattggaa agcatcaagg ctcacaacaa acgtaaattg gctcgtcact
     3301 tgaaagaaca ccaaggtgtg gaaatcaatt caaattctat ctttgatatc caaatcaaac
     3361 gtcttcatga gtacaaacgc caacaaatga acgctttgta cgtgatccac aaataccttg
     3421 acatcaaagc tggtaacatc cctgctcgtc caatcacaat cttctttggt ggtaaagcag
     3481 ctccagccta cacaatcgct caagacatta tccatttaat cctttgcatg tcagaagtta
     3541 ttgctaacga tccagcagta gctccacact tgcaagtagt tatggttgaa aactacaacg
     3601 ttactgcagc aagtttcctt atcccagcat gtgatatctc agaacaaatc tcacttgctt
     3661 ctaaagaagc ttcaggtact ggtaacatga aattcatgtt gaacggagct ttgacacttg
     3721 gtactatgga cggtgctaac gtggaaatcg ctgagttggt tggagaagaa aacatctaca
     3781 tcttcggtga agattcagaa actgttatcg acctttacgc aaaagcagct tacaaatcaa
     3841 gcgaattcta cgctcgtgaa gctatcaaac cattggttga cttcatcgtt agtgatgcag
     3901 ttcttgcagc tggaaacaaa gagcgcttgg aacgtcttta caatgaattg atcaacaaag
     3961 actggttcat gactcttctt gatttggaag actacatcaa agtcaaagag caaatgcttg
     4021 ctgactacga agaccgtgac gcatggttgg ataaagtcat cgttaacatt tctaaagcag
     4081 gattcttctc atctgaccgt acaatcgctc agtataacga agacatctgg cacttgaact
     4141 aatactcttc gaaaatctct tcaaaccacg tcagctttat ctgcaacctc aaagcggtgc
     4201 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtataagata
     4261 caaatttata ctaatacatc ttgtaaaaaa gcgagtttcg attgaaattc gcttttttaa
     4321 atgctgtgga tttgggtcaa tcttgtctaa aaatagggaa atcctagata cagtgaaggc
     4381 tttaaatgct ggtttttact gtcttcagcc ttatattttt tcgtagttgg ttacctcata
     4441 tctcttgtat tcgcttacat aaagtattat aatataattg taggaaagaa ggtgttttta
     4501 tgatatacac acttaaattg gtgttgttta ttacctttct tgtaataagc ttgttacctg
     4561 ataagatttt tggaaaaaat aaaaaaattt ggaaaatagt ttttgcaata ttgacggcag
     4621 tggcagcatt gtcatttatg tactaagtta ttttaagaat gtagggaaat aaaccctaca
     4681 ttctttttag ttttttctgt tttctaaatt ctatttatcc aagcgcttca acatttcttg
     4741 cttcttcgct tcaagttctg cacgcttttc ttcgatttcg gcatgttttt tctcgagttc
     4801 agaacaactt gcatcattgc taaattcttt tcgccatcag gagatagggt gagtcgacat
     4861 gtctattact cacccaaagc agtcctacaa agcaggaatt ttctgttact tttttggaaa
     4921 tagtaacgtt tatacagctt tgacacttcg tatcaaagcg ccaaacacac tccgagggat
     4981 ttacagaaag cagaaaagga atgatctggt ataagatcat tccttttctc tctttttctt
     5041 taagtaatta tatacaatgt acgacgaagt cgtcattgca atgctgatcc accacctaaa
     5101 gggaacttta aacaacattg ataagataaa gaatataaac aacgaaaata cgttataccc
     5161 aattaatttt attgtatatc tcatgattaa aagttaatcc ttccgttgtt aggaatggca
     5221 tcatttttat cccataattg tgctaaataa gtccccggtg ataataaatt catagcgaat
     5281 tctaaagcaa catcatttac aaaccaacta cctagatatc tagaaattgc tgaacgaata
     5341 gcactttttg ctgcatgttt tccttttact ttaattagat ttgcaaggcc tgcagtagtt
     5401 cctcctaagg ctaaagctat tgcagtatct aatagagcac ccatttgatt aactgtaata
     5461 ccttgccaag ctgctctaaa tggagagtat gtaggtggga ttgtataatc gccttgtaat
     5521 tgtcggttaa ttacttcttt gatccattgt tgtgagacgt ctggatgaaa agattggatt
     5581 tcgtttgcaa gtgtattgat ttgttcttct gttagagaag tgacaggttg aagttccata
     5641 tttgtttcaa tttgcgatac ttgttcagaa gcgtatacag ctgaaacact tggaatcgct
     5701 gatacaatta acacaattga cgtcaaaaaa ccgaaataaa tttcattaat ttgttcatga
     5761 gcttttctcc tttttatttg tatctgctta cattttatta tatactgtta ttatagtcaa
     5821 gaaaaatatg ctattatgtt aaaaaaatat ttttcaaaat ataaatggac ggatttattt
     5881 tggattttat ttgttatttt gacctgcctc tatattggta accatgattt gtttactctc
     5941 aatcatcaag aattctcttt tcgtggtagc ttttggggtt tggtactgac cttatatcac
     6001 ttattgttca ttgataagtt tgtaatttcg aatcgaaaat aaagattaga gctatgcttg
     6061 actgtgtact tttaggattg attttggagg aagattttgt ctctattatt tattatttta
     6121 aatttattta ttttgtataa gatctattct ttgaggcggg aaaaatcgaa atacttgatt
     6181 tatacggcct atatcatatt tggggtaaat gtagtatatg gtattcaatg gttattaaaa
     6241 gaactgattt caactatttc cccttaatgc gtatggaaac tataacaaaa cgcataagat
     6301 taaggtttgt gtacctgttc ttatacgttt ttgtcatatt gaagattttc ggtaagtttt
     6361 ctacttttgc gaagtctagt ttatactcaa tgaaaatcaa agagtaaact agccgtaggt
     6421 tgctcaaagt acagctttga ggttgcagat aaaactgacg aagtcagctc aaaacatggt
     6481 tttgaggttg tagatgaaac tgacgaagtc agctcaaaac atggttttga ggttgtagat
     6541 gaaactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc
     6601 agtaaccata cgtacggcaa ggcgacgttg acgtggtttg aagagtatta gaatgctttc
     6661 ccaaccagaa tctctgcttc gatggtaatc tctgtcagtt ggctccagtc aatcttgctc
     6721 tggtcaacgt gaaagaggag aggagtcata ctgagtaggg cttggagttg ttctgctgtg
     6781 atagtcttag tcagagaggc agtttgacta gataggatgg taaagtgttc ctggaaatgc
     6841 tccttgatat cttggttaga atactccttg tttgtcagct ggtcctgtac tctttgacgg
     6901 atttctttga ggtgattttc agttgggata acctttatca agataccgtc tttggataaa
     6961 acgcgacgaa attctccata gttggcaggt gagaagatat caagcagaat atccatgtta
     7021 gcgtctttta taggaaggcg tgccaagtcg ccaacgaacc aattgactgc ccagttgggt
     7081 tcacttttag cagcgatttg gactgaatct ttggagatgt caaaggcata gaaagttttt
     7141 tcagagtgac tttcttgtag tttgcgagaa tagaatcctt caccacaacc gatatccaaa
     7201 attgttgtgg tagtttttga gcttgcaagc aagtcagata cagcatctaa gatagcttgg
     7261 taaaagccgg cttctaggat ttgttgacgg ttttgaaaat tttccttgtc atagttagca
     7321 gattgcttga tttgaggtgc tagattgaca tagccaaatt tcgccaagtc aaaagaatga
     7381 cggttgcagc acttgaaatt agtctctaac agagtcagat tttcttgaca gataggacag
     7441 gcaaaggcag tcgcagaagc aaaacgctga agtttgggct tgagatttgt attcatagtt
     7501 taagtgtatc aaaagaggca aatgaaagca actttaggaa taagtagatg ggagattcag
     7561 tagaaataaa actaattctc caatttatag aatgaaattg cttttatatc taaattgcta
     7621 tataataatg ataaggagga aataagtatg ttaaaagaag tattaaccgt tgcaaaagtt
     7681 gcgaaaaaat cttcattatt tttgggtggt gtcgcatttg gtacccttgg tttgaaaatc
     7741 ttagcaagta aggaagctaa aaaaggttat tctaaagctt tggctaaggc ttacaagttg
     7801 aaagacgagc tagatgcatc tgtttctgtt gtgaagcaac atggagacga tgtcttgcaa
     7861 gatgccaaat atttgtacga gcaagagaaa aaagaagagc aattagatag ccttataggt
     7921 gaataatatg tcttttaaag tgctacatag aggataccaa catatccgac tatcatcttc
     7981 tttttcactt accttggata ttcaagacta tcttcgttcc ttggcgagag atgaaaaggg
     8041 gattgagtct atccagtttt acatggatca acagcacttt actctacgca taaaagaagg
     8101 cttttctgta ttagataatg cagaagcctt tttaaaaaga attgataaag ggaaagtttc
     8161 tgagttgatg actcttccca ttcgtagaga agagagtgct tattctattg tttcaggtgc
     8221 agcggttaag cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct atatatggac
     8281 ttgttatcag gctttgggtt atattagaga agcctatcaa acactagcgc gtaaggaact
     8341 aacgatggaa gtcttggact gttcggctat tttattgtcc ttgtttatga accaatccaa
     8401 gacagctagc aatatcatgt ttatgcttga tttggggaat catttagatc agtggtcctt
     8461 gaaaaaaact gcaacagatt tagaacagag tcttcttgca aaagagagcg atgtattcct
     8521 agtacagggc gatacggttg ttagtataaa gagttccgat gttcaaatag gagatgtctt
     8581 gatcttatct caaggaaatg aaattctgtt tgatgggcaa gtagtttcag gtttaggtat
     8641 ggtcaacgaa agttccttga caggagagag ttttccagtt gaaaaaagag agtctgattt
     8701 ggtttgtgca aatacagtat tagaaactgg agagttacgc attcgtgtaa cagataatca
     8761 gatgaacagc cgtattttac agctgattga gttgatgaag aaatctgaag aaaacaagaa
     8821 aacgaaacaa cgctatttca tcaagatggc ggataaagtc gtcaaatata atttcttggg
     8881 ggctgggctg acttacctat tgacaggttc tttttctaag gctatttctt tcctattggt
     8941 cgatttctcc tgcgctttga aaatctctac tcctgtagct tatttgacag ttatcaagga
     9001 agggttgaac cgtgaaatgg tgattaagga tggagatgtt ctggagaaat atctggaagt
     9061 tgatactttc ttgtttgata agacaggaac aatcacaact agttatccta tagttgaaaa
     9121 ggtgttacct tttggagact atagtgagga agatattctc agaatcagtg cctgtcttga
     9181 ggaacacatt tatcatccta ttgctaatgc catcgtcaag caagctgaga tagagggaat
     9241 tgaacatgag gaaatgcatg ggaaactcca atatatcgca agcaagggga tcaaatctca
     9301 tatagatggc caaccagttc ttattgggaa ttatgtcttg atgcaggatg agcagattca
     9361 tatcagttca gaacaaaatg ctttaattga agagtacaag agtcactaca atctcttatt
     9421 cttggcttat caaaatgaat tgattggaat gttctgcatt catactcctt tgagaaaaga
     9481 agcaaaaaca gccttggata aacttaaggc acaggggaaa aaattgattc tggcaacagg
     9541 agacaccttg attaggacag aggaattagt caaagatttg ccctttgatc aggtctatac
     9601 agacttgaaa cctgatggga aatttgagtt agtagagaaa ctgcagaaag caggtcacac
     9661 tattttgatg gttggagatg gattgaatga ctcagcggct ttaactctat cagatatcgg
     9721 tgtggtgatg aatgagagtg cagatatttc taagcagatg agtgatatct tattgttaga
     9781 taatcgtttg gatttcttcc aagagttgga ttcgttatca tcatctttgc aaacactcat
     9841 caagaaaaat attcaagata ccgttgtcgt aaatagtagt ttgattggct tcggcttatt
     9901 taattggctc agtccttcaa atctctctat cttacataat ctaacaacct tgcgcattgt
     9961 cctgcgtagc ctgtctatta aaaatagata gatgagagtt attaacagca aaaaggagtt
    10021 acctttctcg aggttaactc ctttgtttat agataaatgt tgaatagttt agtaagtcaa
    10081 tacaaagtat tttttcttcc cacgacggat aacagtcagt tcattttcta acttatcagc
    10141 gtcactcaag acatagtcaa ggtcttggat gcggtcgccg tttacgtaga tggctccgtt
    10201 ttggacgtct tcacgggctt ggcgttttga gttaactata ccagatgaga cgagcagttc
    10261 cacgatattg ttgttttcgt ctgcctgtac ttggtagttt ggcacaccac gaagtccttg
    10321 tttgagctct ttgacagaaa ggtttttgat gtttcctgca aagagttgct cagtgatgtt
    10381 aagtgcttct ttgtaggctt cttctccgtg aacaagtgta acaacttcac gagccaagac
    10441 tttttgagcc aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat cttcaatctc
    10501 atcaagtgac aagaaagtaa agattttcaa gaagcgaaca gcgtcagcgt ccatcacgtt
    10561 catccagaat tggtacattt cgtatggaga agtcttttcg ggattgagcc agacggcatt
    10621 tccttctgat ttaccaaatt ttttaccagt tgcatctgtg attagtggaa cagtgataac
    10681 gtgaccagtc ttgtccgcct tacgacgaag caattcggta ccagctgtca tatttcccca
    10741 ctggtcagaa ccaccgattt gaagagtgac attatggtct tggttaagga cgaagaagtc
    10801 gtacccttgc atgatttggt aagcgaactc agtgtaagaa attcctgttt cgatccgttt
    10861 tttaacagat tccttactca tcatgtagtt gaccgtgaag tattttccaa tatcacggag
    10921 gaagtcaatg aagctgatgc tgccaaacca gtcgtagttg ttgaccatga cagccttgtt
    10981 ttcgccattt tcaaagtcaa gaaaacgaga aagttgtcct tggatagact tgacccagcc
    11041 atctactgtg tcttttgttt ggagactacg ttcagcatct ttgaaggacg gatctccgat
    11101 gagacctgta gcaccgccaa cgagcgcata aggtttgtga cctgctagtt gcaagcgacg
    11161 acttgtcaag attgcgacaa ggtggcctag gtgaaggctg tcagcagttg gatcgtagcc
    11221 agtataataa gaaacttgac cttcttctag ggctttacgc aaagcttctt catcagtcgt
    11281 ttgaaatatc aaaccacgct cttttagctc atcaaaaatg tgcatgtgtc ttttctcctt
    11341 tttaaaatta ttgtttctac ctattgtatc acaaacttgg gcaatagcct agcagaatag
    11401 ggaagaaagt ggctatttta ttgaaaattt cccttttgtg gtataataga taaagtgagg
    11461 aaatccatgc aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt tttccagcaa
    11521 aaacaaaaaa ataaaaaatc agctagacct ggcaagaaag gttcaagtac caaaaaatct
    11581 aaaaccttag ataagtcagc cattttccca gctattttac tgagtataaa agccttattt
    11641 aacttactct ttgtactcgg ttttctagga ggaatgttgg gagctgggat tgctttgggg
    11701 tacggagtgg ccttatttga caaggttcgg gtgcctcaga cagaagaatt ggtgaatcag
    11761 gtcaaggaca tctcttctat ttcagagatt acctattcgg acgggacggt gattgcttcc
    11821 atagagagtg atttgttgcg cacttctatc tcatctgagc aaatttcgga aaatctgaag
    11881 aaggctatca ttgcgacaga agatgaacac tttaaagaac ataagggtgt agtacccaag
    11941 gcggtgattc gtgcgacctt ggggaaattt gtaggtttgg gttcctctag tgggggttca
    12001 accttgaccc agcaactaat taaacagcag gtggttgggg atgcgccgac cttggctcgt
    12061 aaggcggcag agattgtgga tgctcttgcc ttggaacgcg ccatgactaa agatgagatt
    12121 ttaacgacct atctcaatgt ggctcccttt ggccgaaata ataagggaca gaatattgca
    12181 ggggctcggc aagcagctga gggaattttc ggtgtagatg ccagtcagtt gactgttcct
    12241 caagcagcat ttttagcagg acttccacag agtcctatta cttactctcc ttatgaaaat
    12301 actggggagt tgaagagtga tgaagaccta gaaattggct taagacgggc taaggcagtt
    12361 ctttacagta tgtatcgtac aggtgcatta agcaaagacg agtattctca gtacaaggat
    12421 tatgacctta aacaggactt tttaccatcg ggcacggtca caggaatttc acgagactat
    12481 ttatacttta caactttggc agaagctcaa gaacgtatgt atgactatct agctcagaga
    12541 gacaatgtct ccgctaagga gttgaaaaat gaggcaactc agaagtttta tcgagatttg
    12601 gcagccaagg aaattgaaaa tggtggttat aagattacta ctaccataga tcagaaaatt
    12661 cattctgcca tgcaaagtgc ggttgctgat tatggctatc ttttagacga tggaacaggt
    12721 cgtgtagaag tagggaatgt cttgatggac aaccaaacag gtgctattct aggctttgta
    12781 ggtggtcgta attatcaaga aaatcaaaat aatcatgcct ttgataccaa acgttcgcca
    12841 gcttctacta ccaagccctt gctggcctac ggtattgcta ttgaccaggg cttgatggga
    12901 agtgaaacga ttctatctaa ctatccaaca aactttgcta atggcaatcc gattatgtat
    12961 gctaatagca agggaacagg aatgatgacc ttgggagaag ctctgaacta ttcatggaat
    13021 atccctgctt actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt caagggttat
    13081 atggaaaaga tgggttacga gattcctgag tacggtattg agagcttgcc aatgggtggt
    13141 ggtattgaag tcacagttgc ccagcatacc aatggctatc agaccttagc taataatgga
    13201 gtttatcatc agaagcatgt gatttcaaag attgaagcag cagatggtag agtggtgtat
    13261 gagtatcagg ataaaccagt tcaagtctat tcaaaagcta ctgcgacgat tatgcaggga
    13321 ttgctacgag aagttctatc ctctcgtgtg acaacaacct tcaagtctaa cctgacttct
    13381 ttaaatccta ctctggctaa tgcagattgg attgggaaga ctggtacaac caaccaagac
    13441 gaaaatatgt ggctcatgct ttcgacacct agattaaccc taggtggctg gattgggcat
    13501 gatgataatc attcattgtc acgtagagca ggttattcta ataactctaa ttacatggct
    13561 catctagtaa atgcgattca gcaagcttcc ccaagcattt gggggaacga gcactttgct
    13621 ttagatccta gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa accagggaag
    13681 gtttctgttg aaggaaaaga agtagaggtc acaggttcga ctgttaccag ctattgggct
    13741 aataagtcag gagcgccagc gacaagttat cgctttgcta ttggtggaag tgatgcggat
    13801 tatcagaatg cttggtctag tattgtgggg agtctaccaa ctccatccag ctccagcagt
    13861 tcaagtagta gttctagcga tagcagtaac tcaagtacta cacgaccttc ttcaagggcg
    13921 agacgataat tctctaaatg aagtggccaa tcaatggatt gccacttttt tcctttcgtg
    13981 ttacaataag ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct
    14041 cctatccggc ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta
    14101 tcaggtgtct tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact
    14161 catttggtct gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa
    14221 ttacctgccc tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta
    14281 tctctgtgta gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt
    14341 ctttatcctc ttttataatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt
    14401 ctatgttttg gttgccatgc tgggtgtttg cttgatggcg acaaagggag atctctctca
    14461 gttatccatg acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt
    14521 taatgttatc ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg
    14581 ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt
    14641 tactcttgat atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt
    14701 tgcttttttc atttccatga aggctgtgtc cttggtttct cctttggtgg tttccgttat
    14761 cagtgccagt gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt
    14821 ggattggtcc ctccttctag ctatagcctt gattatttta cccatgatat ttttgtctat
    14881 agaagaagcg aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact
    14941 agaatagtca ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta
    15001 ggggcttgct tttaccgaca gcacgtttta tctcacaacc ctaaaaaatc atacctaaaa
    15061 acagacaaaa aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga
    15121 accaggaggc acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg
    15181 ccaatgctga aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg
    15241 tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg agactggaag gacgtggctc
    15301 cgcttcttga aggtttggta gaaaatcaag attatgttgt cgagcacgat gctcgtaatt
    15361 ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta
    15421 tccgtgacca ggtggaaatt ggtgacaatg ctgttatcat gatgggagct gttatcaata
    15481 tcggtgctga aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg
    15541 ctatcgttgg aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc
    15601 cagctagtgc tgaaccagtc cgtgtcggag acaatgttct tatcggtgct aatgcagtgg
    15661 ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc agcaggagct attgttaccc
    15721 aagatgtccc agaaaacgtg gtagtagcag gtgttccagc tcgtattatc aaagaaattg
    15781 atgcccaaac tcaacaaaaa acagcgctag aggatgcgct tcgtaccttg taattgtaaa
    15841 agtaaaaaag aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag
    15901 atgttagatt tgattcagac tagacgagat ttacaccaga taccagagat tggcttggag
    15961 gagttcaaga ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat
    16021 tttgttcaaa ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg
    16081 gagcgaacca ttggttggcg gacagatatt gatggcctgc ctatcgtcga acaaacaggt
    16141 ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct gtggtcatga ttttcatatg
    16201 accattgcct taggctgtct tgagcgcgcc cttgaggagc aacccaagaa taatctgctc
    16261 tttctatttc agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggacggt
    16321 gcttttggag attggttgcc aaaccaattc tatggtctcc atgttcgtcc agatttgaaa
    16381 gtcggccaga ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc
    16441 cgtttcaaag gcaaaggtgg ccacgcagct tttcctcatg aagccaatga cgccttggtg
    16501 gctgctagtt actttgtgac ccaagtgcag tcagttgtta gccgcaatgt caacccaatc
    16561 gagggagcag tggtgacctt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca
    16621 gacacagctt ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg
    16681 caaaagaggg taaaaacagt tgcagaaggg gttgcagctg cctttgatat ggaagtcgaa
    16741 gtggaactca aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa
    16801 ctgatggact tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg
    16861 acaggcgagg actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt
    16921 atcgatagtc cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct
    16981 attggggtgg ctgcggtgtc tagtttcctg aaaaagaagg cagcagaata gagggcttgt
    17041 atatgaaatc ggaattacgc aagcaagtct tgcatgaaat gaaggcttta tctcaagaac
    17101 aaaaacaggc tatagaccaa gctttaaccg agcgaatttt acaacacccc ttttatcaag
    17161 aagccaaggt catcgcaacc tacctctctt tttctcatga gtttcaaacg cgggaactga
    17221 ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc caaaacttat cccaaggggc
    17281 gcatggactt tgtagtctat gatccgcagc agttggtaaa aactgccttt gccttactgg
    17341 agccacaggg agatttggaa gtggtggatg tatctcagat tgatttgatt catgttcctg
    17401 gcctggcttt tacgacggat ggatatcgga ttggatatgg tggaggttat tatgaccgct
    17461 atctggaaca tttttctggt cgtactttga gtacggttca tccttgtcaa attcaggact
    17521 ttatacctga aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt
    17581 gatagacgtt accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta
    17641 atgttggtca ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc
    17701 atgtatgggc cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt
    17761 tttgttcata ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga
    17821 aggcaggtag aggagatttt cggctctaag cagttctttt ttctctatct cttatcagga
    17881 atgatgggca atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct
    17941 acctctcttt atgggctatt tgccgcgatt attgttcttc gctatgcaac tcgcaatcct
    18001 tatatccaac agctagggca atcctatctg acactttttg tggttaacat tattggaagt
    18061 gttctgattc caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt
    18121 ctagcagtta tctttccagt tagaggagaa aaacggatgt ataacaccag ccagagatta
    18181 ggagcggtag tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg
    18241 tgacgactaa tactcaatga aaatcaaaga gcaaactagg aagctagccg caggttgctc
    18301 aaagcactgc tttgaggttg tagatagaac tgacgaagtc agctcaaaac atggttttga
    18361 ggttgtagat gaaactgacg aagtcagtaa ccatatatac ggtaaggcga cgctgacgtg
    18421 gtttgaattt gattttcgaa gagtataata tgtaaacttg aataagaaaa aagctactta
    18481 aaaaatgagt agcttttaat gtgttttgga accattcgaa acaacacagc tctaaaatag
    18541 ttattagcgt tgtggaggtt ctgcaatgcc tcccccagtg tttccaagat aattccaatt
    18601 ttcgtcaaac acatctccat tgctctcatt gtcgtaagta taacttacat gtccatcagc
    18661 accaatattc ttatattttt tatagtagcc attatttact ccgctgttgg aattgctaga
    18721 tgaatattgt tctgatgatc caccatttgt tgaactatct ccagaaccag aataactttg
    18781 acttccataa ttattcgaat atccattagt ttgttcagat gaactataat ctacatttga
    18841 attggaagat ccattataag actcattaga agcaccacta gaagaattac tttgactaga
    18901 agattccttt gctttttcag cagctttttc ttcttctttc ttcaaaagtt cgatttgttt
    18961 ttgatattta tcaacaagat catttagaga tttaagagtt tcttccaaat tctcttgttt
    19021 aaaagtttca tcaacttttt tgataaaatc aatttgtttt gtaatttctt tcacatttgt
    19081 ttcaagagtt gctttatctt taaacttctc atcagaatct ttaacatcat aagccaaacc
    19141 tttttgcaaa tgaaaatcta caattgcttt agatgattct ttgactttgt tattaagata
    19201 gtctttacct gcatcggttt taagtgaagt ttcagatagt tcttttaatt tagaaataga
    19261 ttcatctgaa gatgcatcaa atgattcaaa tagtttatca acttgttctt tgtctgattt
    19321 gctatctttt tctaattgta caatttttgc ttgttgttct gcttgttgtt ggttatatga
    19381 ataccatcca aaccctaata ccaatacaac accaactgca actgatgaaa tgattacttt
    19441 tttattgttt tcgataaatt ctacaaattt gttctccata atcttttact ttgatagtgt
    19501 ccgcaattac tttaaaatta aaattacacc tatctttctc ccttattata tttttattaa
    19561 tagcttccac aaaattaaac aacttatgtt cggctcggtt gatttaattt aaccatgact
    19621 actccaacag ttaagtcaaa tttaaaatta tttattattt tggaaccatt agaaacaaca
    19681 tagcaaagtt aaaataaggc tttgtccgta cacaacttga aaaagtgcgc accgattcgg
    19741 tgcttttttg ttttactata ctaatttatt ctactcttat tttaatgaaa aaacaaatac
    19801 aagtatagcg ttttcattag tttttattaa ataggtagat tcaagtagag aatttactta
    19861 atttatgtgt ggctaatctt tatataaatg attttgttat tccttctcag tcaattcttt
    19921 tccaagttgg atgaggtaat tcttcaaatc atctttgact tgtggatgtt tgagggcgta
    19981 gtcgatggat gttttcatga agccaaactt gtctccgaca tcgtaacgag cccctttgaa
    20041 ctcacgagca aatacacgtt gtgttttatt gagggtgtcg attgcatctg tcagctgaat
    20101 ttcatttcct gcacctggag cttgcttttc gagaatctca aaaatttcag gcgtgaggag
    20161 gtagcgtccg ataatagcaa ggtcgctagg agcgtcctct ggagctggtt tttcaacaaa
    20221 ggtttcaaca ctgtaaagac catcttttcc ttcgccttgc ggagcaataa ccccgtaagc
    20281 agatacttcg tcatgaggga ctggcatgac agcgatagta gacgcgtggg tacgctcgta
    20341 gtcatccatg agttgtttgg taagtggaac agccttttcg tctgtgatat ccatcaagtc
    20401 atcaccaagc ataacgacaa aaggttcatt tccgacgaaa gccttggctt gcaaaacagc
    20461 atctccgaga ccgcgtggat gagtttggcg gataaaatgc agacgcatgc cagttgtttc
    20521 atcaactagc ttcaaaagat ctgttttccc tttttctttg aggttatatt ccaattcgaa
    20581 gtttgaatca aagtggtcct caatagaacg ttttgattta ccagtgacaa ctagaatatc
    20641 ttcaatacct gatttgagag cttcttccac gataaactgg atagttggtt tgtctacgat
    20701 tggcaacatt tctttggcaa gggccttggt tgctggtaaa aatcgagttc ctagtccagc
    20761 agcagggatg actgcctttc taacttttga tgtcataaaa atcctttcta tagagggtta
    20821 agaccactca ttttctgctt taaattcatt gttcatgatg tcataaatgg catctttgat
    20881 attggttccg tgataaataa cttggtaaat agcctgtgta atgggcatat agactccaag
    20941 ttcttgggct agttcatagg ctgctcgagt cgttgaaatt ccttcgatta ccatgcccat
    21001 attagcttct atatcagcta gggattctcc tcgtccgaga gcatctccag ctctccagtt
    21061 acgagagtgg atggaagttc ccgttacgat caaatctccc acaccagata agccgctata
    21121 ggtcaatgga ctggccccga gtgctacccc taggcgggtg atttctgcta aacctcgagc
    21181 gatgatggct gccttagcat tatcaccaaa tccaagacca tgtaaagctc cagcaccgac
    21241 agcaataata tttttaagag caccagcagt ttcaaccccg ataacatccg tattggtata
    21301 aagtcggaag tagtgattac taaatagctt ctgaacgtat tgagctgttt gtaaatcttt
    21361 agaagcagca gttattaaag ttaggtcacg cacaatggtc tcttctgcat gactaggccc
    21421 tgaaacaacg acgatatcac tacggagatg ttcaggaatt tcttcttcaa gaatggttga
    21481 taatcgttta tggctatcag gttctaatcc ctttgatgcg tgcatgatga taaccttatg
    21541 gtccaaggtt tgtgcaactt gctgggcaac aagtcgtgtc acttttgttg ggacaacaaa
    21601 caaaatcgca tccacatctt tcaatgtttc tgctaagtcg gtgtaggcaa tgatattttc
    21661 gtctagaacg acatctttaa agtagtgctt attagtatgg tgtgtattaa tttcattgat
    21721 ttgctcggga agatttcccc aaatacgtac ctcgtgtcca ttgtcattta agacttgtga
    21781 aagggcggtt ccccaagaac caggccccaa gacggcgacg gtttgttttt ccatgatttc
    21841 ctccttgata gagttctttc tcttattcta tcatattcta ggggattttg cacttgcatt
    21901 actggggtat ggtggaaaaa tacacaaaaa ccgagacgaa gggataaact cttagtcttt
    21961 agttaaagtg agaagaaatc tgatagatag tttgattcag tatatgctta taatagaggt
    22021 aaacaactca ggaggttctt gtaggttgcg agagtttggc gaaaaaatta aaagattacg
    22081 tttggctaaa aaaatcagtc gttcagaatt ttgtggtgat gagtctgaat taagtatccg
    22141 tcaattaatt agaattgaaa atggagaatc cagaccaaca ctaacaaagt taaaatatat
    22201 cgctgaacgt ttgggggttg aggattacaa gttgatgcca agttatatag agttggataa
    22261 ggaataccta gaattgaagt attttttgat gaggactcct acatacgaag atgaaactat
    22321 cgcccaaaag aaagagagtg ttttgataag atttttgaag agtattatga taggctacct
    22381 gaggaagaaa gatttatcat cccaaattat tcatatctgg cactaacgaa ctacacagtt
    22441 caaaaattac cagaaaagct agttgaaata ctgtccttct ggtgattttg cttaatttct
    22501 gatgttcgag acataattga ggagatttgt cttgattttt tcctaaaaaa gggcagactt
    22561 ctcccttggt ttgatgctcg atttttaaaa taaggtgact tattctgtta ggcatgtttt
    22621 cccctgccct ctagctctat atggaacagg gagtatcaga tttatctggc agattgtatc
    22681 aaataaggct tgaaactgtt ttgaataggc gtatagactt gagaatttga gaatatgtcg
    22741 tctagcagta gagacatatt ttccggcaat atgaaggaag agacgtcgaa aacgcttgat
    22801 agtcaaggac tttacttcat caccagctag ctgttttaaa aagaggtaga gattataagc
    22861 cagacagcct atcatcatac gtacttcatt cttaatcatg gtcgaactat ctgtcttatc
    22921 cccaaagaat cctgctttcc tttctttgat aaagttttca gcattccctc gttcacgata
    22981 gaattgaaac agctcctcac tatctacact gcttaaattg gtcaaatgaa cttttttaca
    23041 accataaaaa agagcactta atcgtactct tctcttttaa ggattagttg aattaattca
    23101 aatccactag ttctcctgtt tctaggtaga ctaggcgttc acaaatgtta gcaatgtaat
    23161 cagcgaagcg ttccagatgc cctatgatat aaaggtattg tgttccattg ggaattgagg
    23221 tttcttggtc tttcataagt ccaatgattt cctttgataa ggcataataa tattggtcaa
    23281 tctgttcatc tttttgagca atactaatag cttttgaggc ttggtgcaaa ggaaaggcaa
    23341 ccaataaatc ggatagcatg ctgagggata atttacccat ttggtgtaac tgttcttcgt
    23401 caggggctag ttgattttct tttagttgca aaacagcttt agcaatgcct gccatatggt
    23461 ctcccatacg ttcaaggtct gaacaagaag acatgatgct aatcacaaat cgaaggtcag
    23521 acacttgtgg ctgctgcaag gccaacaaac gggcacaggt caattcgata gcgctttgac
    23581 cttggttgat agcatgatcc ttattgataa ttagctctgc catctccttg tctttggagg
    23641 ctaaggccag taaggttttt gaagctgttt caaggacaag ttgccctagt cctaaaaagg
    23701 attgttctaa ttcatgcaat tctaagtcaa attgatttct cataggtttt tccttcctta
    23761 tccgaaccgt cctgaaatat agtcttctgt gcgctgatct tttggattgg taaacacgtc
    23821 aacggtgtct ccaaattcgc aaatttctcc tgttaagaaa aaagcagttt tatctgaaat
    23881 acgtgaagct tgttgcatgt tatgggtaac aatgataatc gtataatcct tttttagttg
    23941 ctgaatgagg tcttcaattt ttaaagtgga gatagggtct aaggctgaag tcggctcatc
    24001 cattaacaga acatcaggtt ctactgctaa agctcgcgca atgcaaaggc gttgctgctg
    24061 accgcctgat aaggacatgg cactcttttt aagattatct ttgacttcgt cccaaatggc
    24121 tgcccctttt aaagattttt ccactaaggc atctaattgt tttttgtctc gaataccatg
    24181 tgtccttggg ccataagcca cgttatcata gatagacatg gcaaagggat taggctgttg
    24241 aaaaaccatc cctacacgct tgcgtagctg attaaggttg aatttgctac tataaatatc
    24301 ttgctcatct aagaggactt ggccttcaat atggcaagaa ggaaccaaat cgttcatccg
    24361 attaagggtt tttagaaaag ttgatttgcc acaaccagat gggcctatca aggcagtaat
    24421 ctgtctttct ggtaattgaa tcgaaatatt ttttaaggct tgaaaatccc cgtaaaataa
    24481 gtctaggtgt ctgactgaaa atgttcccat actaggaagc tcctttcaca agtttccgag
    24541 ataataagct tgatagagta tttatcatta aaacagtaat aatcaaaatc acgccggtag
    24601 cataggcttc attgacatgt agcccctcac ttgacagcat atacatatgt agggctagag
    24661 aacggcctga agacatgaga ctacttggcg tattggtaga ggtacctaat gtatacatga
    24721 gggcagctgt ttcaccaacg atacggccaa tagctagtat cactccagct aaaatacctg
    24781 gcatggcaac tggtagaaca attctaaaaa cagtccgtaa cttacctgcc ccaagtccat
    24841 aacttgcttg acgcatacta tcactaacag ataaaagggc ttcttctgtt gagcgaataa
    24901 tgactggcaa caccatgata actgaggtta agattcctga taacagagag tattgaaaac
    24961 ctaagaagac tacaaagaag agcatgccaa acagaccaaa aacaatggaa ggaatcccag
    25021 ataaggtatc tgaggccaat cgcatgattt taacacaaag ggaatctttt tttgtatatt
    25081 ccacaagata aaaaccagca aaaatcccta tgggcaaggc taaaagaaga gcaccaaaga
    25141 ccagaataac ggtggaaata atcgctggca taagggaaat gttctcagaa gtataagtcc
    25201 aagaaaagag ggatagactt agatgaggta agcctttgat gaggataaaa ccaatgatta
    25261 aaaagagaga gccaaaggtt aaagctgaaa aacaataaac gagaagtttt agcaggtatt
    25321 tactcataag atgattttcc tttcaagtag gcaaagtagg cattaatcaa gagaataagg
    25381 aaaaagagaa ctgctgaggt tgcaataagg gcttccctat gctgacctga tgcgtaagcc
    25441 atttccagaa caatattggt tgttaaggtt ctggttcctg aaaagagtcc acttggaata
    25501 atcggctggt tgcctgccac caaaataact gccatggttt cacctactgc gcgaccgatt
    25561 cctaaaataa ctgctgataa aataccagat ctcgcagctg gcaagatgac actaaaaata
    25621 ctccgttcat gactagctcc tagagccaag ctaccagaat aatacgtttt gggaactgtt
    25681 cggatagcag attctgacaa actgataatg gttggcaaaa tcattattcc taatagtaac
    25741 gaagcggtta ggacactcat gccatttcct aaaaagcttc taatccaagg caccaataat
    25801 tgtaggccga aaaaaccata aacaatagat ggaatggctg ccatcaagtt gatagctgat
    25861 tttaagaagc catagacggg ctttggacaa taataaacca taaacaccga tgtcaagatg
    25921 cctgttggca ccccaatcac aatcgctcct aaggtaatta ataaggaacc aacgatcatt
    25981 ggtaaaatac catagcttgc cggaatgttc gttggcgacc aatcactgcc taataaaaaa
    26041 cgggcaaagc cgtagttagc tatgaaaggt aagccattac taaaaataaa gaaacagatt
    26101 agcaaaatag ctacaacagc tactgttgca ctcatgaaaa aaattgccct aaaaactgct
    26161 tctttgaagg cttgttttgt cacatcttgt cctttctagt gaagaaagta agggagatac
    26221 gacacctccc tacttgcctt ctttatctta ttgtacgatg aaacgtctgc atctctttag
    26281 agatttatgg agcaaacatt ttatttaatc ttgtcccagg tggttaattt gccactaaaa
    26341 acgtctgcaa gttcagccat actgacttgg cttgccttat tgtcattatt gaccacaaca
    26401 gcaataccgt ctaaagcaat agcatcatgg gtgagactct taccttcttc aggagttaat
    26461 tccctagaaa ccataccaat atcagcggtt ttctccttaa cagcggtaat acctgctgaa
    26521 gacccattag aggtaatatc aatcgtaact tctggatttt cttttttata agcttctgct
    26581 aatttttcca ttaaagaaga tactgaagtg gaacctacaa cagacaactt gcctgataag
    26641 tgttggcttg tatattctgt ggtttcggtt ttagcttcaa taaatttatt atcagtgacc
    26701 acttgttgac cttgtttgga gtggataaag ctgataaaat cttgacctag cttggaaaga
    26761 ttagaagacc aaacaatgtt gaagggacgt tgaagagggt attcaccatc taaaactgtg
    26821 tctcgactag ccttgacacc atcaatctct aaagccttga cagatttcgt taaagatccc
    26881 aaggagatgt agccgatagc attagcattc ccttgaactg ctgagagaac accttctgta
    26941 ctattttgaa tcacagctgt tttggcagtg ttgtcaattt ttttatcacc gtcttttttg
    27001 agaatccctg tgatttctgt gaaggcaccc cgtgttccag agccattttc tcgtgaaatc
    27061 acctcaatcg ttcctgaagc tgactgtttg gaagcagctg actgattgcc acaggcaaca
    27121 agcccaaatc ctgataagcc aatggctgca agagtaagca tttttttgaa tttcataata
    27181 atcaccttta tctctatgta tttttcttgt gtaggcttac tacatttata gtctaacaag
    27241 tctttgtaaa ggtttatccc tgattcatgt aaagattgtg taaagaatca aaaaaagcca
    27301 cttttgaaaa atggctgctc ctaaaaatag ctttaaaaat tattagtcct gtgcgaaaga
    27361 ttggttagga agaaaaatcg tgaagcaact gcctctgcca agctgactcg tcaccgtgac
    27421 ttggccacct aataattgac tgagttcttt gacaatggca aggccaagac cagtgccacc
    27481 agtttgtctg cttcgacctt tattaactcg gtaaaaacgt tcaaaaatac gatcctgctc
    27541 taattgacta ataccaatcc ctgtatctga tacagaaatc ttaatgcctt cgttcacctt
    27601 ttgggtcttg acctcaattt ttcccccttg ttcagtgtaa cggatggcat tggataaaag
    27661 attgagtaag atttgggaaa gtaattgact atctgatacg agggtgacat catctggcac
    27721 ctgcaccttt agctgtaaat ccttcttctt gagctgaggt tgcaagcttt gagtcaaatc
    27781 ctgtacaaat tctgccaaag aaagggtcgt ccattgtata ggcatttgtt gagccttaga
    27841 taaggtaaga agatgctcaa caatatgctc aagacgcaag ctttctttgt aaataatgtc
    27901 tagaaagtca tccttgagcg cttcttcttc agctgacatc cccttaatgg tttcagcaaa
    27961 gcccttaatc gaagtaactg gtgtcctcaa ttcatgggag gcatttgaga caaaggctaa
    28021 atttaacttt tcataagttc taatcgttgt taaatcatat agcaagacga gcacagcttc
    28081 cacagattgg gtggggctaa aaacgggaac tgctgtcact tctaaaatca agtcaccctc
    28141 atgaaaccca cttacttctt gttttaacct tgttttttga tcaaaggctt ggtgaactaa
    28201 atttcgaata tccatccgtt tgaggtcatc aagtgaactt atgtcgccgt ccacatcggg
    28261 aaaataatga ggcagagagc gactggataa taacatctga ccttgagcgg aaactaaaaa
    28321 cgtccccatg gttaggtgcg acagaagaac ctccattgtt tcggctaggt ccttgtattg
    28381 ctgatcctgt tgggagactt tggtttttag gccagacaca tactgagcca aagactttaa
    28441 gtcttcttgc cctttttcta aaaagtattc actactggtc aagagaggtt ggtgcaaggt
    28501 ctcaaaagca acttcccatt tccaaaggca aaagagccag tagccacctt gtcccaaaga
    28561 aagggctaga agaaagagac cgatgccttt actgatccaa gttaatgcca tccctgcaat
    28621 cagaatgagg ctaacactta gattgactag ccaaaattga aggtagcgtt tcatctataa
    28681 ctccttgaac ttataaccat aaccccgaat ggttcgaata aattgagggg ctttaggatt
    28741 gtcttcaatt ttttccctca acttaccaat atgaacgtcc accaaacgtg tttcctgccc
    28801 aaagtcatac ccccagatac gttccaaaag acgctctcta gttagtgtca tgttgggatg
    28861 tttcataaga tagagcaaga gttcaaattc ttttggggtc aaactcagta acttattcgc
    28921 cttgtagact tcatgacgct cagggtatac tttcaaggtc ccaaatagcc aagaatcgtc
    28981 agcgatatta tctgaatcat ctccttcttg ttctccttta gttcgcctga ggacagcctt
    29041 gacacgcgcc agcaattctc tagggctaaa aggcttggtc aggtagtcat cagcccctaa
    29101 ttccaaggcc aaaaccttat caaattcatc acttttcgca gaaaccatca taattggagt
    29161 tttgacgcct ttggctctca gtcgcttaca aacttccatg ccatctaatt gtggtaacat
    29221 gatatcaagc aagataaaat caaagggttc tgtttctgcc aaagctaagg ccttccgtcc
    29281 atttgttacc aattgagtag aaaagccttc cttacttaaa tggtagtcaa gcaatttcag
    29341 aatgtgttct tcatcatcca ctaataagac ttgttttgtc atctattatc tcctattggt
    29401 aacattataa cacaattatc agaaatccta acattgctaa attagattaa atttgcttat
    29461 caagactagt atctggtcaa atgctcaatc atctccttgt gctctggata ggtcgccagt
    29521 agatctgccc tttcaaataa ttcaaaatcc tcaaattcaa aaccaggagc aacaagacaa
    29581 gaaaccagag cataatcctt atcaactgtt gatccccaaa tagtgctctt aggaacacag
    29641 tagtgaagtt gttgcccttt ggatatgtcc aggcctaaag tgactgcttc gtagtgacca
    29701 tctgctgtaa tcatgtgaac agtaagtggg gatcctgcat gaaaatacca gatttcatct
    29761 gctgtcaatc ggtgaaaatg tgaaggattc gtttcttcta ataagaaata aatactggta
    29821 taaagcgccc ttcccttacc agcaaggttt atagtgtctg aagctttttt tgtttgtcta
    29881 aaatagccac cttcaacatg gggagctaac tctagagttc ttatcaagtc ttctttatcc
    29941 atcggggcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc
    30001 ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtacaaatc aatcgcccta
    30061 aaaaaagaat tagcgaatga ttttggtaaa aagaaatttc acgctatgaa ggctcaagcg
    30121 attgtcacaa gtcaagggag aattgtttct ttggatatcg ctgtgaacta ttgtcatgat
    30181 atgaagttgt tcaaaatgag tcgcagaaat atcggacaag ctggaaaaat cttggctgac
    30241 agtggttatc aagggctcat gaagatatat cctcaagcac aaactccacg taaatccagc
    30301 aaactcaagc cgctaacagc tgaagataaa gcctataacc atgcgctatc caaggagaga
    30361 agcaaggttg agaacatctt tgccaaagta aaaacgttta aaatattttc aacaacctat
    30421 cgaaatcatc gtaaacgctt cggattacga atgaatttga ttactggtat tatcaatcat
    30481 gaactaggat tctagttttg caggaagtct attatttggt taggtgaatt agtgaagcgt
    30541 ttaggcaagt gtctctggtt acgacgtaaa gaagcataaa tcgatcatat ttatgtttct
    30601 ttactagtga aacgcctagc caaattacca tagttacgac gtcatgacct ctaaatcgat
    30661 tatatttagg ggtcatgact agtgaagcag ttagctagtt cgctagttcg catataagcg
    30721 gctagcgtct aacaattagg aactttagtt ccaataactt taagattacg acgttttagg
    30781 acataaatcg atcatattta tgtcctaaaa ctagtgaagc gcctagccaa agtccgaata
    30841 ggatttggcg ttagttactt agattgcttt gcaatcaagt aactttggcg atttacatct
    30901 tctctggcgc ttctactcca agcaagcgaa gggcttcttt gagaacgact gcggttgcgt
    30961 agctgagggc tagacggctg tcgcgttctg ggctttcatc caagatacgt gtatgtgcat
    31021 agtatttgtt aaaggattga gctaggctaa ttgcaaattt agcaatgata gaaggttcaa
    31081 agttatctgc cgcacggttg ataatacgtg ggaagtcttg aatgagttta atgatttccc
    31141 agctttcagt atcattcaag ctatagttgc cagctgtttc tggtttgaaa tcggctttgc
    31201 gtaagataga ttggatacga gcgtaggcat attgaacgta aggtccagtt tcaccctcga
    31261 aggataccat agcctctagg tcgaagtcgt atccatttgt acggtcggtt ttgaggtcat
    31321 agaatttaat ggctccaacc ccaacagcat gtgctacttg gtctttgttt tctagttcag
    31381 gatttttagc ctcgatttgg accttggcac ggctaacagc ctctgcaaca gtaggctcta
    31441 gcaagatgac attcccttta cgagtagaga gtttcttccc ttcttttgta accaaaccaa
    31501 aaggaacgtg agtaatgtcg tcactccagt cgtagcccat ctcttgcaag acagctttga
    31561 gctgtttaaa gtgggcagat tgttcttgac caacgacata gatagattta gcaaattggt
    31621 attcgttttt acggtaaagg gctgcagcca agtcacgtgt gatatagaga gttgcaccat
    31681 cagatttctt gatgagagct ggatgttcaa ttccgtattt ctcaagattg acaacttggg
    31741 caccttctga ttcaagaagt agtccttttt cagaaagaat gtctacaact gcatccatct
    31801 tatcattgta gaaggcttct ccgttatagc tgtcaaattc aaccttcaat tcattgtaaa
    31861 ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg agagcttcct
    31921 catctccatt ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag ctagggtcat
    31981 tttcagcttc agcgttgatg cggacataga gtttaaggag ttcatcgatt ggatgagctt
    32041 ttacagcttc ttcgtcgccc cattttttgt aggcaacaat caacatccca aattgtttac
    32101 cccagtctcc caaatggttg accttgaccg tttgataacc gattttttgg aaaatatgtg
    32161 acaagctatc tccgataaca gttgaacgca ggtggccaat agaaaatggt ttagcgatat
    32221 tcggactaga catgtcgata acaacatttt cttgtttgcc aatattttgg tcagcatagt
    32281 gttctttttc agtggtaaca gcttgcaata cttgagcaga aatggcagat ttatcaagga
    32341 aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg ttcatttttt
    32401 cagccagttc agccgcaatc atttgtggtg ctttacgttc gactttggca agagaaaaag
    32461 cagggaaagc gatgtcaccc atttctgagt ttttaggggt ttccagtaac tttaaaatag
    32521 cctcttggtc caggttatca atgatgctag ccaattcgct agcaatcaat tcttttgtat
    32581 tcattaagag ctcctttttg gacttttcta ctattttaac acaattttaa agaaagaaga
    32641 aaaaattttt gaaatctcct gtttttttgg tataatatgg ttataaatat agttataaat
    32701 atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa aaaaaatgat
    32761 tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg cgcacaatgt
    32821 ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa
    32881 gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg atttggtgga
    32941 atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg tgcttcatac
    33001 caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaacaagg atgaatggat
    33061 tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc agcacgttgc
    33121 caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc ttgggagttg
    33181 ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc
    33241 ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt gctctttcgg
    33301 atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca gattctggaa
    33361 atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc gggtgttccc
    33421 tatcattctg cccaacagta tatcgatgtc ttgattgagc agggttataa ggtggctatc
    33481 gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga ggttgttcag
    33541 gtcattacgc cagggacagt ggtcgatagc agtaagccgg acagtcagaa taattttttg
    33601 gtttccatag actgcgaagg caatcaattt ggcctagctt atatggattt ggtgacgggt
    33661 gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat ccgtaacctc
    33721 aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca aatcctcagc
    33781 cgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct tcatttattg
    33841 gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca gtatgttcat
    33901 cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga aattaaggat
    33961 ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa tgctcgctca
    34021 ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc tatggggatg
    34081 cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat cgtccaacgt
    34141 caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt gacagacagt
    34201 ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat
    34261 ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt
    34321 ttagaaggga tggagcaacc tactctagcc tatctcatcg cacaactgga tgcaatccct
    34381 gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt gattacagat
    34441 gggggaatta tccggactgg atttgatgag actttagaca agtatcgttg cgttctcaga
    34501 gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc tggtatcagc
    34561 acgctcaaga ttgactacaa taaaaaggat ggctactatt ttcatgtgac caattcgcaa
    34621 ctaggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc agaacgcttt
    34681 ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc
    34741 aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta catccagcgt
    34801 ttacaagctc tagcccaagg aattgcgacg gttgatgtct tacagagtct ggcggttgtg
    34861 gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat tgatatccgg
    34921 aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat tccaaatacg
    34981 attcagatgg cagaagatac cagtattcaa ctggttacag ggccaaacat gagtgggaag
    35041 tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg ttcctatgtt
    35101 cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat cggagcagca
    35161 gatgacttgg tttcgggtca gtcaaccttt atggtggaga tgatggaggc caataatgcc
    35221 atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg tggaactgca
    35281 acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga gcacatcgga
    35341 gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc tagtttacaa
    35401 cacttggtca atgtccacgt ggcaactttg gagcaggatg ggcaggtcac cttccttcac
    35461 aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa gattgctggc
    35521 ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga gaatcaagga
    35581 acagagagtc ctcctcccat gagacaaact agtgctgtca ctgaacagat ttcactcttt
    35641 gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt gtataatatg
    35701 acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata aaaccaagac
    35761 tcactagtta atctagctgt atcaaggaga cttctttgac aattctccac ttttttgcta
    35821 gaataacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta
    35881 ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg caggccctgg
    35941 cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg cgttatctgc
    36001 aggaagtctt aggcgccctc cttactggga aatatgaagc tatttatagt atcggggctt
    36061 ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag
    36121 cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt aaaattcaaa
    36181 ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt cgaatgacaa
    36241 atgatatcaa ccagattcag aacgttgtca tgatgacctt ccaaattctt ttcagacttc
    36301 ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct ctgtggtggg
    36361 tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg
    36421 ggcctcgttt tgccaagttt caaacccttc ttgagcgcat caatgccatt gccaaggaaa
    36481 atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt
    36541 ttacagaggt ctcagacgag cttcttggtc aaaaccttta cattggttat gccttttcag
    36601 tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct atttggctgg
    36661 tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct tttgttaatt
    36721 acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat tctgtcagcc
    36781 gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct
    36841 tcaaggatat cccagatgaa gagttggttg gaagccttag ctttgaaaat gtgaccttta
    36901 cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc
    36961 aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct caattgattc
    37021 cacgtctctt tgatccacag gaaggggcta tcaaaatcgg aggtaaggat attcgagaag
    37081 tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta
    37141 gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta tttgaaatgg
    37201 agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg
    37261 aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga
    37321 ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg acctcagcct
    37381 tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg aaggggacga
    37441 caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc
    37501 taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg
    37561 tttaccgtga aatctatgaa acacagaaa
//
LOCUS       DAVLTM010000006        37320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLTM010000006 DAVLTM010000000
VERSION     DAVLTM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.006"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..1179
                     /locus_tag="VYN16_000251"
     CDS             <1..1179
                     /locus_tag="VYN16_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402840.1"
                     /translation="GVAGKAGADGRTPYVHFAYSENADGSGLTMTDNGQRYFGHYSDY
                     EKPDSSDKTKYKWADRWAKVDGGYVNIYALSKNRSIGKSYHVSEFNMDVLSGNITLKA
                     IGSDPYIGAVSSHPGIFIKQQGMKIPVIQGRSICITITNPLFRKNYISFFNSLGKTVK
                     TYKHYNTNKFLISSADLVGVEFIALRYGAGSSNIQIGTVLETKVKVEYGTVHSDWSPA
                     PEDIESNINSKADQGLTQEQLNALNEKSQILEAEMKAKASMEAFSELEKAYNAFVKLN
                     ADSQKKSESDLVEAGRRIDLLTTQFGGLAELKTFIDTYMKSTNEGLIIGKNDASSTIK
                     VSSDRISMFSAGKEVMYISQGVINIDNGIFTASIQIGRFRTEQYHLNKDVNVIRYIGG
                     "
     gene            1190..3268
                     /locus_tag="VYN16_000252"
     CDS             1190..3268
                     /locus_tag="VYN16_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural protein"
                     /protein_id="HEV1402841.1"
                     /translation="MTKFINSSGSLHLNIYIEQVSQDIANNSSRVSWKATVDRDGAYR
                     TYTYGNISNLSVWLNGSSVHSSHPNFDTSGQEFTLASGEVTIPHSGDGTKTFAVWASF
                     DPNNGVHGNITVSANYTLSSIPRSSSVSDNALSGNRRLGSPHTLTIDRKSSLFTHQVW
                     YRVFGSNWIDLGKNHATGVSFVPNIDLARYNTKAKSGTMDICVRTYNGTTQVGNDVYS
                     NGWYFEIPESVKPTFSGITLTDMNTVARQLLSGNNFLQIISDIQVNFNNPSGAYGSTI
                     TGYRAEIVNKNQVITVNGGRLGMMNFNGSATIRASVVDSRGRQSDTRDITINVIEYFA
                     PAFSFTAFRTREIPNIIQVVRNAKIAPITLSGSQKNVMTLSFKVARLGSTTFTADHGR
                     ASGIWTTQHTLNNSAANMAGNYVATKSFVVIGTLSDKFTSTEFTATVATESVVMSYDK
                     DGRVGIGKVAEQGGAGSLDVLGDIYARNKPIQQYQLTDNNGCGKLIKQDFNTMKETGT
                     WWINGSSQNNPFSGTWGMLEVFRPNPGSHERIQRFTTSTGYMAVRENGYDNNWRPWRY
                     LVQQSKSTNNSDYVALLKSESTPTPWQNAILQNGWNHHRDYGGVQFSKTFDGVVCFKG
                     TCKGGKIARESIILTLPEHFRPSTTLFKTALNNDYGSAVIGIYPNGNVVVKSNVDATW
                     LNFDNVFFKI"
     gene            3328..3591
                     /locus_tag="VYN16_000253"
     CDS             3328..3591
                     /locus_tag="VYN16_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402842.1"
                     /translation="MLKVTKTRQLVTEFFAQDGDQQKLVKTTVINTDNKAVSTISETL
                     HDPELYANNRISMRKHEQELREMRYKIEDAILAELEADAEHKE"
     gene            3601..4017
                     /locus_tag="VYN16_000254"
     CDS             3601..4017
                     /locus_tag="VYN16_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1402843.1"
                     /translation="MKIEFFNFLRSVVQTEDGLVLYALTLIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSICLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            4021..4353
                     /locus_tag="VYN16_000255"
     CDS             4021..4353
                     /locus_tag="VYN16_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1402844.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            4357..5313
                     /gene="lytA"
                     /locus_tag="VYN16_000256"
     CDS             4357..5313
                     /gene="lytA"
                     /locus_tag="VYN16_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1402845.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(5619..6761)
                     /locus_tag="VYN16_000257"
     CDS             complement(5619..6761)
                     /locus_tag="VYN16_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1402846.1"
                     /translation="MWMEELPNGKFKYFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKIKQKLGEKQHSVSNITFEKLYEEFEENWKHGVKNSTVYASKNVKKEILKQIE
                     GDYLVRNIDRRLLQKVIDQLLQDGRSHNYVSKIKFKLNQIMKFAIRMNYIDTNEMLFV
                     ETPRKVITSDELRKKNTKYLDQKEFKLFIQNLKDEALCDYRITKYIRIAKVLFLTGMR
                     YGELAALNYKEDIDFSKKTIHIKHTYDFRQKERTTPKTIKSDRVITAPQKVLDIIKEQ
                     IIENATNGFDTDFIFINTLGEPITNARVICALKRHGQKIGIEKNITTHTFRHSHISLL
                     AELGIPLTAIMDRVGHSDSKTTLEIYSHVTQKMVSDISSKLDKVKF"
     gene            complement(6880..7230)
                     /locus_tag="VYN16_000258"
     CDS             complement(6880..7230)
                     /locus_tag="VYN16_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402847.1"
                     /translation="MNIIAIIIIVIFVGGVIGAVIDNQKKSPEQRERELETFRANQEK
                     KKQEKKQNIITCPNCKSKDVTFLQQDKKAFSVGKAVGGAVLTGGVGALAGFAGKKGNK
                     QWHCQNCGNFFETK"
     gene            complement(7244..7624)
                     /locus_tag="VYN16_000259"
     CDS             complement(7244..7624)
                     /locus_tag="VYN16_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1402848.1"
                     /translation="MTEKEFSQNLGIDIEIFEDGLFPDEAFYIPALKTMFLSDAISDE
                     KRVQVALHEIGHRNHAPDTYQLFREKCELEANRNMIHHLMKAELDIAEDATTFNYLVF
                     MEKYNLKTIADEIMVKEEYLALLN"
     gene            complement(7646..8002)
                     /locus_tag="VYN16_000260"
     CDS             complement(7646..8002)
                     /locus_tag="VYN16_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1402849.1"
                     /translation="MTILDRIRSLANDRKVTLAELERNLNFSNGSLRKWETSTPSGDK
                     IEKIADYFNVSTDYLLGRTDNPTIANKKEQFFFEGKEVDVEELASTAMRFNGKPLTEE
                     DKKAIQNIIEIYLRKQ"
     gene            8278..8463
                     /locus_tag="VYN16_000261"
     CDS             8278..8463
                     /locus_tag="VYN16_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1402850.1"
                     /translation="MIYDKIKEIASEKGISIYRIEKDLDLGNGAISKWNNSSPSATTL
                     NSIANYLNVRLEQLLEE"
     gene            8465..9163
                     /locus_tag="VYN16_000262"
     CDS             8465..9163
                     /locus_tag="VYN16_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEV1402851.1"
                     /translation="MNELINVTLNDNQEPVVSGRQLHEALGVNSRYTTWFDRMKEYGF
                     TEGQDFLPNLGKSTGGRQATDHIIKLDMAKEIAMIQRTERGKQVRQYFIQVEKDFNSP
                     EKIMARALLMADQKVHKLEAQIEADKPKVLFADAVSASHTSILVGELAKLISQNGYKI
                     GANRLFSWMRENGYLIKRKGSDWNMPTQRSMDLKLFEIKETNVQHADGHITVNKTPKV
                     TGKGQQYFIDKFLN"
     gene            9349..9690
                     /locus_tag="VYN16_000263"
     CDS             9349..9690
                     /locus_tag="VYN16_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1402852.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVNRKTIDKYFKIFEEAGYLRK
                     IRKKPPGNGGSHIFRFFSDVKISDFQFDIMKQRLNLSIKRASMNYNSDIPKSEMSESE
                     MSESEMSDFGH"
     gene            9847..10113
                     /locus_tag="VYN16_000264"
     CDS             9847..10113
                     /locus_tag="VYN16_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402853.1"
                     /translation="METVQIVRIKDVIIEKISANDEELERIFGCSKRQAGDMRREMKK
                     LPSQQKYLRNDGQLVTIKGFDAYLQYRGSQSWKKEMAKTVKMTR"
     gene            10197..10649
                     /locus_tag="VYN16_000265"
     CDS             10197..10649
                     /locus_tag="VYN16_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402854.1"
                     /translation="MPNWAEGTLKLRGRRENVASALKEMLLGDEGATLEEEYDGTLLR
                     FKNEYDYFYINGTRRAFISSKDIEIWLDDDFVIIELEDFEQAWAALADNYTEISSKFD
                     VDIKIFTFEMGMEFTQEIEISKGEIIKNIVNENFTNYSWDVPFSRLGG"
     gene            10655..11356
                     /locus_tag="VYN16_000266"
     CDS             10655..11356
                     /locus_tag="VYN16_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEV1402855.1"
                     /translation="MADLTFAELQRKMQIEKQTKQGVKYPFRTAEDINNKFKSLDSGW
                     SVSFPEDDIIQKGDKLYYKAVAVAKRESDGTIEKAIGWAREEDVPIFHTQKGDVKQMQ
                     DPQWTGAVGSYARKYALQGLFAIGGEDVDEYPVEESQEQGQNNQQQKPNNQQAQGQNQ
                     VRYIDNIQYQEINDLINDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGDYEVLKN
                     YLTEQLAKAKAKRGN"
     gene            11359..12351
                     /locus_tag="VYN16_000267"
     CDS             11359..12351
                     /locus_tag="VYN16_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1402856.1"
                     /translation="MVKDVTNSLTEIKVDFQPAVINVDREAIEAQVAAAIAQYSGREV
                     TVDNYKEVYEERTRFNKLIGGLDTQRKDFNRQINEPAKDFDKWVKEKVIKPIEGVTDA
                     MSAGLNAIDEHERLMRVDVVRATFEDKCMVAGIEKSTFADKYDEYSLKKYFKTGKYEL
                     KKTTLDEMDGLVLSEFDALEEYKANKQAIQEQAQEYDLPADSYIRHLEDGKSLVDILK
                     MMKTDRDAEIARKEQKEAQEKAKAERLEEIAQSAKKNANANIKAYDAETGEILEQGTI
                     TPEPQNNAREVAKFEPSEPLVKLVRLELHGGLEQWENTQEYFEDNFIGFETLED"
     gene            12373..12549
                     /locus_tag="VYN16_000268"
     CDS             12373..12549
                     /locus_tag="VYN16_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402857.1"
                     /translation="MRCFYVSGKIADLDLGSEINAENSFMAAIEFVKRYTGLLKFGSN
                     EIKVSEVEEVQNDK"
     gene            12539..13084
                     /gene="ssb"
                     /locus_tag="VYN16_000269"
     CDS             12539..13084
                     /gene="ssb"
                     /locus_tag="VYN16_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1402858.1"
                     /translation="MINNVVLVGRLTRDAELRYTQSNIAVATFTLAVNRPFKNEAGER
                     EADFINCVIWRQSAENLVNWAKKGSLIGVTGVIQTRSYDNQQGQRVYVTEVVASNFQL
                     LESRNSQQNNQGHQDHHGGYQQQGYSNQGSSFQNGNSYGQQGSFLEGNTTNLVPDFTR
                     DNNPFGRPTNPLDISDDDLPF"
     gene            13110..14249
                     /locus_tag="VYN16_000270"
     CDS             13110..14249
                     /locus_tag="VYN16_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1402859.1"
                     /translation="MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDT
                     KGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRER
                     VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLV
                     GTLCARDYKEPKQVAIPVLTPDRVNKRQNGRRLKKNGEPMFTLTAQDRHGILIKEATK
                     KGYAEATVGDSVNLSHPNSKTRRGRVGNQIANTLLTGESQGVVEPDFRIRKLTPRECW
                     RLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVISAIAQGLGGN"
     gene            14284..14487
                     /locus_tag="VYN16_000271"
     CDS             14284..14487
                     /locus_tag="VYN16_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402860.1"
                     /translation="MTKIEIVMVLTTLMSITWAAIVTIHTMQAIKKHKAKVDYYQKPQ
                     VQCEIARHVLKNKWYSDGGEVFR"
     gene            14541..14975
                     /locus_tag="VYN16_000272"
     CDS             14541..14975
                     /locus_tag="VYN16_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402861.1"
                     /translation="MDKKLIGLDLTHIADGGLQEKLDKELEKVFDNILDLNTDAKAKR
                     KVTITLTMSANEERTVVDTTMEVKSKFAPQNGVATTILIGRDFDTGQVHANELKSTVP
                     GQMYFDENGEILTDIGQPVAEIEQQAETKPDIIDFNKKKVGN"
     gene            14977..15714
                     /locus_tag="VYN16_000273"
     CDS             14977..15714
                     /locus_tag="VYN16_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402862.1"
                     /translation="MTTENLKAALEYAVELNEHGLEILTAADGTEYYDANKFNLKELD
                     PKRYPKTLELSTLTSLVDYLKTDLNNLKNQRLIVAVEKNDEVCVWSENDELERRTLLV
                     DVKARIPELSFGRFLSSEQFNIMLQSNFIDDNDRGTLLEFASALKIENGAEIEDNGVS
                     QVATVKTGVASLAKGKAPNPVTLRPYRTFSEVEQPASLFVFRIDKQANMALFEADGKR
                     WVADAVGNIAAYLKEQLADQKLITVLA"
     gene            15731..15943
                     /locus_tag="VYN16_000274"
     CDS             15731..15943
                     /locus_tag="VYN16_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402863.1"
                     /translation="MSDYKQRMIEEYKQLKERTNKLSLMISNYYVGTLDFKLKCPIEL
                     LETQHYTMCAYLKILEQRAEIENIEF"
     gene            15954..16355
                     /locus_tag="VYN16_000275"
     CDS             15954..16355
                     /locus_tag="VYN16_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402864.1"
                     /translation="MKFEFSLPRNTKLKSLNMVINSNDRQHQTDKAKVTKRIRAFAYW
                     HTSMNKDKGRAAFSPSNPCEVTVTIYSPTKSKLDPPNLYPTVKAIIDGMTDAGIWTDD
                     NHKVIKKLSFVYGGLSEEKGHYRLEFDIEEV"
     gene            16357..16563
                     /locus_tag="VYN16_000276"
     CDS             16357..16563
                     /locus_tag="VYN16_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402865.1"
                     /translation="MIEVNIKFDNFEAHGFYQDDTKLGKIRDAIISQMNNGHVVILGE
                     DRSILLNPKVIKCVQFEVVEDDQI"
     gene            16613..16927
                     /locus_tag="VYN16_000277"
     CDS             16613..16927
                     /locus_tag="VYN16_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402866.1"
                     /translation="MEELKKKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWIGYAPSMAQVVIAVYLIYR
                     GN"
     gene            16929..17429
                     /locus_tag="VYN16_000278"
     CDS             16929..17429
                     /locus_tag="VYN16_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1402867.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPE
                     KVKVPQFMADWIDYFKKNGDWDLFQAMDYLFGKKEIREWLEYKNNQDLFARAWLDGYE
                     VEKEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDAKFGWVFDCPGI
                     EIEEVK"
     gene            17429..17722
                     /locus_tag="VYN16_000279"
     CDS             17429..17722
                     /locus_tag="VYN16_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402868.1"
                     /translation="MTQTLEEGMKNQSKCIKIPMEIRPFDIGYRIVNQHGNPLALKNG
                     ASIFDLPSLAEKAIKKEFSKDDPDFDIEKHFVEEVAIVNLSKFHSYFEEVIHD"
     gene            17722..18114
                     /locus_tag="VYN16_000280"
     CDS             17722..18114
                     /locus_tag="VYN16_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402869.1"
                     /translation="MTKKKIERLSVIHRREINWLKWYFLRDKKNPKRTILEQKIIVSH
                     IKTDRLEAKFLTNLKKSTEDFIDGSDPKYLRAIKEVYVYENMNVIGACQKILFYSPTQ
                     AYVLLNAWFNDYFRATYTELLENAILDK"
     gene            18180..19274
                     /locus_tag="VYN16_000281"
     CDS             18180..19274
                     /locus_tag="VYN16_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEV1402870.1"
                     /translation="MEMLQIEYVDIKSIKPYHKNARHNDGEATEKVAASIKAFGFQQP
                     ILVDDNNIIITGHTRLKAALSLGIDTIPIAHAVNLTDEQIKAYRLADNRVAEYSTWDS
                     ELLNIELSQFETIDMAQFGFELSVTGFNFGNEEEQQEETENEEEDAEDFHRDTTINQY
                     NLFNYDDTRVEGFYNMPKIEGVDHIPKDFQGFNYVLNKPDYSSCVHFFLDDYQFERIW
                     QRPDFYIEKLLEFDSALAPDFSLYLDMPIAMQVWNIYRSRLIGQIMQDYGLTVIPTVS
                     WASEESFDFCFDGLPKNSTLAISTIGVKQNKEQFEVWKNGVTEMIKRLTPKRIVVYGG
                     KVEYDYKDIEVVYFENATTERMKESGTKTN"
     gene            19255..20001
                     /locus_tag="VYN16_000282"
     CDS             19255..20001
                     /locus_tag="VYN16_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402871.1"
                     /translation="MAQKLTKLKDIFKHVSSIDLGKEILFEDLELYNKETETSKQYQS
                     IEEAENDLSLMEKVNKINFTLGGGRGANFEKGKDGKYPGFRGAGGARDSGSSKALHPA
                     SLNNQGRFSSVEGAIQGFIKKHGGSRTEYSTAVDSQGFAHNYVHGGKNSVQILPISGG
                     FTAIHNHPNGSNFSSTDLHSFAALKGMNTLVATNSSKAYRITKGANFDAKGFDKAVSK
                     SRFTTKDYNKGADLWLKKNAKKYGYTYSYE"
     gene            20205..20660
                     /locus_tag="VYN16_000283"
     CDS             20205..20660
                     /locus_tag="VYN16_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HEV1402872.1"
                     /translation="MPRDGTKNLKPVTERTKDEARAISSKGGKASGIARRKKADLKKA
                     FETLLSLDVTDSKIKKQLEEMGMAGNNEALLAFATFQQAVKGNQKATENIIKLTNTKD
                     KYDIQEQKERIKALKYENRERAEAEKGSSETIEIVDAWAEDVRGATDDL"
     gene            20650..21960
                     /locus_tag="VYN16_000284"
     CDS             20650..21960
                     /locus_tag="VYN16_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1402873.1"
                     /translation="MTFNVQKNINPHFKSVWISSLPYNVLKGGRNSFKSSVIVLKLAY
                     MMIRYIIAGEAANIVVIRKVANTIRDSVFNKVWWALNLFGIAEQFTKTVSPFKIVHKT
                     TGSTFYFYGQDDFQKLKSNDIGNIIAVWYEEAAEFNDQEDFDQSNVTFMRQKHPRAKF
                     VQFFWSYNPPRNPYSWINEWFESIKTNKNYLAHSSTYLDDELGFVTEQMLEDIERIKE
                     NDYDYYRYLYLGEAVGLGNNVYNMSMFHAIDALPSDDKLIGISFALDGGHQQSATACC
                     AFGITAKGKVILLDTWYYSPAGQVVKKAPSQLSKEIYTYIRSVIEKYRVQALQYTIDS
                     AEGALRNQMFLDFGLKWHPVAKLRKVTMIDSFQSLLAQGRFYYLNTENNKIFIEEHKM
                     YRWDEKTIKSDNPSVIKEDDHTCDTTQYFVLDNAKLLGLRVGNV"
     gene            21973..23541
                     /locus_tag="VYN16_000285"
     CDS             21973..23541
                     /locus_tag="VYN16_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1402874.1"
                     /translation="MSLFQKVKDFFSRGRYYMQTSNLNSILEHPKIAVTQEEYDRIKR
                     NLVYYQSKWDDVQYKNTDGDIKSRPMNHLPIARTASKKIASLVYNEQATITTKNEILQ
                     KFLDDMLTNDRFNKNFERYLESCLALGGLAMRPYIDGDKVRVAFIQAPVFFPLESNTQ
                     DVSSAAILTKTIKSEGRKNVYYTLVEFHEWVTADGQETGSTNDKKYYRITNELYRSDV
                     NDVLGQRVNLSELDKYKNLEPVTVFENLSRPLFTYLKTPGMNNKDINSPLGLSIFDNA
                     KTTIDFINRSYDEFMWEVRMGQRRVIVPEHLTQRQYQRPDGTIDFRPRFDVEQNVYMQ
                     IGGSSMDAGGITDLTSPIRANDYILAISEGLKLFEMQIGVSSGMFTFDGQGMKTATEI
                     VSENSDTYQMRSSIVALVEQSIKELCVSMCELGKAVGVYSGEIPELDDISVNLDDGVF
                     TDRHAELDYWAKMVAAGFSTKKRAIGKTLNISGVEAEKELNAINSELLPMNDAELAIY
                     GMHDQNEEEADDKG"
     gene            23528..23701
                     /locus_tag="VYN16_000286"
     CDS             23528..23701
                     /locus_tag="VYN16_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402875.1"
                     /translation="MTKVKFGVTSVDYSASIEDTPTIKLGLIIRGSGRLDASSVIKKL
                     IKDVSELEYELEE"
     gene            23744..24895
                     /locus_tag="VYN16_000287"
     CDS             23744..24895
                     /locus_tag="VYN16_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEV1402876.1"
                     /translation="MKKKRKQITFNDQQFPLQMQGVGDIYEKLQIDIFDRMIKRLKER
                     GSIDLMRNPYIWQLEKLNDMHMLNEQNLKLISERTGIAERLLRDVIENEGLKVYKDTK
                     QQLEEDLNKIPEGEISNGVTDSLEAYSRQAVSDLNLINTTLPKSLQVAYKSIVEETVA
                     QVVAGTKTSDVALHDTIMKWQKNAFTGFVDKGGRHWKADSYARAIIKSTTYKVYNEMR
                     TRPAEELGVDTFYYSMKAMARPACSPLQGQIVTKGAGRKIDGITIYSLLDYGYGTAAG
                     CLGIHCGHYLTPFIVGVHELPNLPDYLKNLTPEQAEENARIEAGQRGLERLIKTHKER
                     LHYAHTLQDDKMIQAERLKVRGYQTKIRNLINQHDFLTRDYRREKLYIS"
     gene            25034..25597
                     /locus_tag="VYN16_000288"
     CDS             25034..25597
                     /locus_tag="VYN16_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HEV1402877.1"
                     /translation="MAFTTEELLNLGLTEEQAKSVFALRGKELNEDKSALETIKQERD
                     SLKSQLQKAEEQVEHLKSLENISAEQKDAIDKLQAEYDKYKNEAAAELAQTKKVSAIS
                     LALKDTNAFNPDKLMKFIDVDAIQLDDNGKPQIDEVINGLKESDPYLFKAEESKPSPN
                     ILPQGNPAGEGTSDVDPFQAIIDGYGK"
     gene            25615..26496
                     /locus_tag="VYN16_000289"
     CDS             25615..26496
                     /locus_tag="VYN16_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEV1402878.1"
                     /translation="MPSNQNNAVRRYEKQYAGILETVFRVRAAFSNALASIQILDGVQ
                     ENSKAFSVKTNNTPVVIGEYKTGENDGDFGDNSGAQSRFGGLTEVKYENTDVNYDYTL
                     TIHEGLDRYTVNNDLNAAVADRLKLQSEAQTRTVNKRIGKYLSDNATNTEALADFTDD
                     KVKALFNKLSAFYTNNEVTAPITVYLRSEFYNAIVDMASVTSAKGATISLDENGLPKY
                     KGFTLEETPAQYFETGVIAIFSPNGIVIPFVGISTARVIEAENFDGVKLQAAAKGGTY
                     TLDDNKKAIYKVTGTIV"
     gene            26507..26740
                     /locus_tag="VYN16_000290"
     CDS             26507..26740
                     /locus_tag="VYN16_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402879.1"
                     /translation="MAIYKATKNLFFEQLNMDVIVNDIIELDEDYAKEVNKKLKNAFP
                     DVKNVLELVDKNGTLEPEDAPSVDDASQATVED"
     gene            26783..27175
                     /locus_tag="VYN16_000291"
     CDS             26783..27175
                     /locus_tag="VYN16_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402880.1"
                     /translation="MTYLTQEEFDELDFDEVTDFEKLAKRAKIAIDLYTNGIYQKDID
                     FEKEIAYRKSAVKLAMAFQIAYLDASGIMSADDKQLANSVSIGRTSISYSTSQSTLAG
                     QRFNLSMDAENALRQAGFSLVVGVAYDR"
     gene            27165..27536
                     /locus_tag="VYN16_000292"
     CDS             27165..27536
                     /locus_tag="VYN16_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1402881.1"
                     /translation="MIDKRLLKGIDKRLLKDVLTIKKVADKNDYGDEVYSEPLTIKNV
                     RFDRSVGGSGNRNSKTGTGNSKSRQKQGVIYLYPSLSFVTVDNSWMGAKVNDGIGDYT
                     INGFQTNYYDGEIFSQEIEVI"
     gene            27536..27880
                     /locus_tag="VYN16_000293"
     CDS             27536..27880
                     /locus_tag="VYN16_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1402882.1"
                     /translation="MNIAIKVDLQKAKQKLSNESMTRGKIAVASKILLDNEQYIPLRG
                     GELRASGRIVGQGDAVVYGTVYARAQFYGANGIVTFRRYTTPGTGKRWDQVATSKHAE
                     EWARAFVKGMGL"
     gene            27880..28287
                     /locus_tag="VYN16_000294"
     CDS             27880..28287
                     /locus_tag="VYN16_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1402883.1"
                     /translation="MRENDFQNVLLKHIKTLNLPVEPRFDYFEDDKDDLVINQIPGGK
                     VDREYMDGTQEVSLPFEIAVKAKKNSVANDTIWLVTSELAKIDLVLPSDNNSYEYMGM
                     EVSRPAMKGKDEQGYYYYTIEIVAKIVIERNKQ"
     gene            28284..28733
                     /locus_tag="VYN16_000295"
     CDS             28284..28733
                     /locus_tag="VYN16_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1402884.1"
                     /translation="MTRQKNALRGHFVAPYNGGTEPTTEDTWLELAKWISDVSDDTDE
                     KTDDQAYYDGDGVEETTVVSVKGAYTFEGTYDPDDKAQALIAGMKYKTGDDRKLWHKV
                     VSSDKKKQWVGAATATEIKAGSGAASDYEVFGCKLSYNSTPKETGIG"
     gene            28798..29286
                     /locus_tag="VYN16_000296"
     CDS             28798..29286
                     /locus_tag="VYN16_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402885.1"
                     /translation="MTDIQIELKRTGFPVKIGEVELWFDTSQESLMRFYDMEEELKRR
                     LVQYELDVVSANINNKIERDGVTKEVVAGAIELEKKQLEIQYDLIFGDGTFDKLYSVY
                     PDYNALNNALEQTAIMLHDKLEELAEQHKTVVKERASHYLNKGKVTPIKNNKKQKKNK
                     KK"
     gene            29299..29868
                     /locus_tag="VYN16_000297"
     CDS             29299..29868
                     /locus_tag="VYN16_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402886.1"
                     /translation="MSMKLNDALITNFSIADKEYDIDLSFNKVLDVFEILKEDEMTRL
                     EQAQLIVHLLTGQELYDIKEVVDCWIYIKEHFLGIEKETVQYDLLGNPMPKAKGEEEQ
                     EKLIDFEQDAEYIYASFLQAYGINLLKVQNELTWTEFKALLNALPDNTIMQQIIEIRA
                     WKPEYGGDKNKMRKLQAKYSLGKEGEDND"
     gene            29861..33163
                     /locus_tag="VYN16_000298"
     CDS             29861..33163
                     /locus_tag="VYN16_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEV1402887.1"
                     /translation="MTDGKVTIVVDVDGNKVKVLNDELDKTAQKGDRGSDSLKKFAIG
                     GAAFKLASKAVDLLTDSLGGAIQRFDTLESYPRVMQAMGHSTEDVTRSTKKLAAGIEG
                     LPTTLNEVVGTAQRLTSITGDINKSTDLTLALNNAFLASGSSSADASRGLQQFSQMLS
                     AGKVDMQSWKTLQETMPYALQKTAESFGFAGQSAQNDFYSALKEGRITFNQFSSKLVE
                     LNGGVGGFAELAKTNSKGIQTSFGNLKNAVVKGVANTIKALDDLTKAATGKTIAENFD
                     ALKVIINAAFGVIVNVIKASTPVFQTLFSILGIGASVISFLRPAIIGLVAALVTMRAI
                     NQAVKTTKDLISAWKIFKLTATRAIRIINLLTAAQATYGSITKAQLVAHLANNGALTA
                     SNLLYGVLTGSISLQTAATIAATAATTAFKAGLTAVKAALTALTGPIGLVVAGVGLAV
                     GALVGLWQWLTAESEETKRLKSEQEELVKSTDQLTDSVKQSAKERQKNLESVKGNTES
                     YQKLADEIVQLSQKTNKTAADKKNLKKKIDALNASVSGLNLAYDKNSDSLSHNSDEIK
                     ARISAMEAESTWETSQKNLLDIEQKRAEIGEQLKQIAEQRKKWNEESNVSDGVRKERL
                     QELNDKETELKNTQTELQTEYEKTSQVQQAASEAMAAAAENGSNRQVVAYENMSKSQQ
                     KAIDDMRTKYNELLETTTNMFEQIKYKSAISVDEMIANLQKNQEAVNNWATNLNTLAE
                     RGVNEGILAKLQAMGPQGGLYVQELVNASDEKLATLNEVFTQGGESAMNGLTAGMDTG
                     ALGITDKIKGIVQSQVSSLQEEIAAADFSSLGQEIPNGVSQGIEQGASTAGESSKNMA
                     NDIKESFTSEMDINSPSRVFNEYGGFITTGLAEGVDKGTNQPVSSVTNLANQIKKPFD
                     SLQSDFTYIGEMAMSGLNAGLWSGSGSVMATANSIAERVKATIKSALDIHSPSRAMRD
                     EVGRFIPQGIAVGIEADAGVVEKSMLRLKESMMIDTRPEIALGLNKKLGAQVTVKQSS
                     KQTIAEKIKVTMDKSSELLKKALDVAETAVRRPNEMYLNDGTLVARTGDKFAKYQSEQ
                     LRRDNRMKGVLS"
     gene            33160..34674
                     /locus_tag="VYN16_000299"
     CDS             33160..34674
                     /locus_tag="VYN16_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEV1402888.1"
                     /translation="MTKIMTFNGVDMSKFFRITDIIRPIGNKRSVSTDNAPLLGVNIQ
                     QVKIGEKEHIIKFDIKTTNAIEMEQLKHDLAGILNVLEPVKITYGDEPDKYYMGLPVD
                     EITPENLTRWFQRSELKIIIPDGVAHSTTLKNFDIDTNETSAPDRIVFNLTNTGTEPA
                     YPIIRIKHNSENGYIGVVNNRAAFELGNREEADTEKYRDSETLIDYRGTNILKGFQNG
                     TKGVAVTNDNKERLVGTLSTTSMWGRNHIELSNRGTVEKNRNNAQSLTWAIPVDSSGE
                     VGSLNDYLLWRQVFMAAVANQYGFIKVTVSDTDGNFLYGVETYKRYQTLDCEYSFFTT
                     DGKGGYKFIKWWYFTGTGAQVGKLDPFSAEKGWSELKRNDDRVQVFFDGSHYDFIIPE
                     IKDKKSAKIHITLGALRDWPLVSHMYVDEFMYRKDFVTKSRDIPNRYPIGSNVVINSE
                     DDSVYIDGISKVSEVVDGSHWPAIPPGKSQLELYFSRFVKKKPTVTIEFEERWI"
     gene            34676..>37320
                     /locus_tag="VYN16_000300"
     CDS             34676..>37320
                     /locus_tag="VYN16_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1402889.1"
                     /translation="MLLTIHDANLQKVAFIDNEKQGTLNYYDDTWTRSLATGSSTFEF
                     TVFKKAVKSDLPLAKAYHHLNEHAFVSFKYKGKSFVFNIIIVEENEQTIKCYCENLNL
                     ELINELANPYKSNKAMTFKEYCEAMDLLNYTHLSIGINEISDYKRTLEWEGQETKLAR
                     LLSLAKRFDAEIEFDTQLNADSTIKKFSVNVYHENDDNHQGVGRVRNDVIVKYGKNIH
                     SITRKVDKTGIFNTIRPTGKMPTVEEEPSGDKGSKSETVKNADGSTTKTTISTASDGT
                     KSKTIVHTKVTKLADKTRITTTTTTRSDGSIEQTVTTSKKGGASTSETKVLKKPNPKE
                     KTNTTEDVLTIEGLDEWEVKNEKGIVEFYQRGQALYAPISMQLYPSTFTHSTGELDQW
                     TRKDFHFETDEPNELRRLGYLKLKKYCYPAITYEVDGFVDADIGDTVKVHDDGFAPLL
                     MIQARVTDQKISFTNPVRNKTIFDNFKALENKLSADIQSAFERLFEAAKPYTIKLSTD
                     NGVIFKNQIGQSLVTPTLYKGGKPVVVGVTWRWALDGEVTTGMTYLVRGSNVTDTVTL
                     TVAAYIGNKEVAVDEISLVNVADGKLGTPGTPGRDGRTPYVHTAWANNATGTDGFSLD
                     SSINKLYIGIYTDFEPNDSTDPKKYKWAKVKGEKGEKGDKGEPGQRGLDGLQGARGEQ
                     GLPGRNGADGRTQYTHIAYSNSADGTKDFSVSASDRAYIGMYVDFNRADSNTPSDYNW
                     TLVKGSDGANGVAGKAGTDGRTPYLHIAYATSNNGSQGFSTTDSTNKTYIGTYTDYTQ
                     SDSTDYRVYKWTLIKGADGTGISNVTNYYLATTVSTGITRTSAGWTTTPQPITSDKRY
                     LWNYRVELYTNGTSKTTEPTVIGVHG"
BASE COUNT        12782 a         6212 c         8087 g        10239 t
ORIGIN      
        1 ggcgtggcag gtaaggctgg tgcagatggt aggacacctt atgttcactt tgcgtattct
       61 gaaaatgcag atggatcagg tttgacaatg acagataacg gacagcgtta ttttggtcat
      121 tattcagatt atgagaaacc tgatagttcg gataaaacta agtacaaatg ggctgatcgt
      181 tgggctaaag ttgatggagg ttatgtaaat atctatgcat tgtctaagaa tagaagtatt
      241 gggaaatctt atcatgtttc tgaatttaat atggatgtac tttcagggaa tatcactcta
      301 aaagcaattg ggtcagatcc atacattggc gccgtttctt ctcatccagg tatttttata
      361 aagcaacagg gaatgaaaat cccagttata caagggagat ctatttgtat aacaataaca
      421 aatcctttat ttagaaaaaa ttacatctcg ttttttaatt cattaggtaa aactgttaaa
      481 acatataagc attacaacac aaataaattt ttaatatctt cagctgattt ggtaggtgtt
      541 gaatttatcg ctttacgata tggtgcagga agctcgaata tacaaattgg aaccgtacta
      601 gagacaaaag taaaggtaga atatggaacc gtgcatagcg actggtcgcc cgctccagaa
      661 gatattgaaa gtaatattaa ctctaaagct gatcaggggc tgactcaaga gcagttaaat
      721 gccctcaatg agaagtcaca gattttagag gctgaaatga aagcgaaagc atcgatggag
      781 gcctttagtg aattagaaaa agcatataat gcctttgtga aattaaatgc agatagtcaa
      841 aaaaaatctg agtctgattt agttgaagca ggtagaagaa ttgatttgct gacgacacaa
      901 tttggaggat tagcagagct taaaacattc attgatactt acatgaaaag cacaaatgag
      961 ggcttgatta taggtaagaa tgatgcaagc tctactatta aggtatcaag tgatagaata
     1021 tccatgtttt ctgcaggtaa ggaagttatg tacatttcgc aaggtgtaat aaatattgat
     1081 aatggtattt ttactgcatc aattcaaatt ggacgtttta gaacagaaca gtatcatctt
     1141 aacaaagatg tgaatgtcat acgatatata ggaggttaaa aagagggaaa tgactaaatt
     1201 tatcaattct agcggttcac tacacttgaa tatttacatc gaacaagtta gtcaagatat
     1261 cgctaacaat tcctcaagag ttagttggaa agctactgtt gaccgtgatg gagcttaccg
     1321 cacatatact tatggtaata ttagtaactt gtctgtatgg ttaaatgggt caagtgtgca
     1381 tagtagtcat ccaaactttg acacatccgg gcaagagttt actttagcaa gtggggaagt
     1441 aaccatccca cacagtggtg acggaactaa gacttttgca gtatgggcat cgtttgaccc
     1501 aaacaacgga gtacatggaa acattaccgt atcagcaaac tatactcttt caagcattcc
     1561 tcgatctagt agtgtaagcg acaatgctct ttcaggaaat aggcggctcg gaagtcctca
     1621 cactctcact attgatcgca aatctagctt atttactcac caagtatggt atagagtgtt
     1681 tggtagcaac tggattgatt taggaaaaaa tcacgcaaca ggcgtttctt tcgtacctaa
     1741 tattgaccta gctagataca acacaaaagc aaagtctggc acgatggaca tatgtgttcg
     1801 aacatataat ggaactactc aagttggaaa tgacgtttac tcaaatgggt ggtattttga
     1861 aattccggaa agcgtgaaac ctacattttc tggaattaca ttgactgata tgaatactgt
     1921 tgctaggcaa ctattgagcg gaaataactt tttacagatt atttctgata ttcaggtcaa
     1981 ctttaataat ccatccgggg cttatggttc gactatcaca ggatatcgtg ctgaaatcgt
     2041 aaacaagaat caggttataa ctgtaaacgg tggtaggctt ggtatgatga atttcaatgg
     2101 ttcagcaacg attcgagcta gtgtggttga tagccgaggc aggcaatcag atactagaga
     2161 tattacaatc aatgttattg aatattttgc accagctttt agttttacag cttttaggac
     2221 gcgggaaata cctaacatta ttcaagttgt caggaatgct aaaatcgctc ctatcacttt
     2281 atcaggtagt caaaaaaatg tcatgacact atcattcaaa gtagctcgat taggtagtac
     2341 aacttttaca gctgatcatg gtagagcttc tggtatttgg acaactcaac acaccttaaa
     2401 taattcagct gctaacatgg caggtaatta tgttgcaacc aaatcatttg tggtcatagg
     2461 aactctatct gataagttta caagcacaga atttacagca acagttgcaa ctgaaagtgt
     2521 ggtaatgagt tatgacaaag atggccgtgt gggcattggt aaagttgcag agcaaggtgg
     2581 tgcagggtca ttggatgtct tgggagatat ctacgctaga aataaaccta ttcaacaata
     2641 tcaattgact gacaataatg gatgtggaaa acttatcaaa caggatttta atacgatgaa
     2701 agagactgga acgtggtgga taaatggtag ctctcaaaac aacccctttt ctggaacttg
     2761 ggggatgcta gaggtattca gacctaatcc aggctctcat gaacgtattc aacgcttcac
     2821 tacctcaaca ggatatatgg cagttaggga gaatggttat gataacaact ggagaccatg
     2881 gcgctatctg gtgcaacaat ccaagtctac taataactct gactatgtag ctttgctaaa
     2941 atcagaaagc actccgactc cttggcaaaa tgccatttta caaaatggct ggaatcatca
     3001 cagggattac ggaggtgtcc aattttcaaa aacattcgat ggtgttgttt gttttaaagg
     3061 aacatgtaag ggcggaaaga ttgcacgtga gtcaatcata cttactttac ctgaacattt
     3121 cagaccatct acaacattat tcaaaactgc tctgaataat gattacggtt cagccgttat
     3181 aggaatttat ccaaatggaa acgtagttgt caaatctaat gtagatgcta cttggcttaa
     3241 ttttgataat gtgtttttca aaatataaca accgtaaaaa atccctaatt attaacggat
     3301 aattaattta taaaggagga aatgacaatg ttaaaagtca ctaaaacacg tcagctagta
     3361 actgaatttt tcgcacaaga tggcgaccaa caaaaattgg tcaaaactac tgtaatcaac
     3421 acagacaata aagctgtttc aacaatatct gaaacgctgc atgacccgga actgtacgct
     3481 aacaatcgta tcagtatgcg taagcatgag caagagttac gagaaatgcg ctataagatt
     3541 gaagatgcca ttttggcaga gctggaagca gatgctgagc ataaggaata ggaggtgtgt
     3601 atgaaaattg aatttttcaa ttttctaaga agtgtcgtac agactgaaga tggtttggtc
     3661 ttgtacgctc taacactgat tgtctcaatg gaaatcattg attttgtcac agggacgatt
     3721 gcggcgatta tcaatcctga catcgagtac aagagcaaaa tcggcattaa cgggctcctt
     3781 cgtaagattt caggggttct cttactgatg atcctcattc cggcgtccgt tttgttgcct
     3841 gaaaagacag gttttgtatt cttgcattca atctgtctcg ggtacatcgc atttactttt
     3901 caatctctca ttgaaaatta ccgcaaatta aaaggaaatg ttactctttt tcagccgatt
     3961 gtaaaagtat ttcagcgatt acttgaaaaa gatgatgata cgaaaaaagg agaataacaa
     4021 atgcaacaaa ttactgaaat cattactaat ggagcaatca gcatcctagt cgttttggca
     4081 ggggttgtag ttagggcagt caaggactac ctggttcaaa aaggtggaga aaagaccatc
     4141 aagattgttg aaatcttggc caaaaatgca gtaaatgccg tggagcaggt agctgctgaa
     4201 actggctaca agggagatga aaaactggca caggctcgcg ctaaagtccg tgctgagctt
     4261 acaaaataca atattagtat gactgacaaa gacttagaca ccttcgtaga gtcagcagtg
     4321 aagcagatga atgacgcatg gaaaggacga tagggaatgg atatcgatag aaacagacta
     4381 cgtacaggct tgccccaggt tggggtgcag ccttatcgac aagtacatgc tcactcaaca
     4441 ggtaaccgca actcaaccgt acagaatgaa gcggattatc actggcggaa agacccagaa
     4501 ttaggttttt tctcgcacgt tgttgggaac ggtcgcatca tgcaggtcgg acctgtgaac
     4561 aacggaagtt gggatgttgg gggcggttgg aatgctgaga gttacgcagc ggttgaactg
     4621 attgaaagcc attcaactaa ggaagagttt atggctgact atcgcctcta tatcgaattg
     4681 ctacgcaatc tagcggacga agcaggcttg ccgaagactc ttgatacaga cgacttggca
     4741 ggtatcaaga cgcatgaata ctgtaccaat aaccaaccaa acaaccactc agaccatgtg
     4801 gatccatatc catatcttgc aagttggggc attagccgtg aacagtttaa gcaagacatc
     4861 gaaaacggct tgagcgctgc aacaggctgg cagaaaaatg gcactggcta ctggtacgta
     4921 cattcagacg gctcttatcc aaaagataag tttgagaaaa tcaacggtac ctggtattat
     4981 ttcgatggct caggctatat gctttcagac cgctggaaga agcacacaga cggtaattgg
     5041 tactactttg accaatcagg cgaaatggcc acaggctgga agaaaatcgc tgacaagtgg
     5101 tactattttg atgtagaagg tgccatgaag acaggctggg tcaagtacaa ggacacttgg
     5161 tactacttag acgctaaaga aggcgccatg gtatcaaatg cctttatcca gtcagcggac
     5221 ggaacaggct ggtactacct caaaccagac ggaacactgg cagacaagcc agagttcaca
     5281 gtagagccag atggcttgat tacagttaaa taaatagaaa ggaaactttc taaattgttc
     5341 tttcaccgca ggctcaggct tgcggttttt ttgttttaaa aaaggggcaa aaaaggggca
     5401 aaagtgtcgt aaacctctgt aaaacgatgt aaaaaatcaa ctttgccctc gctttaaagc
     5461 tctaaatttc aacgtattgt gaaacagtgt aaattatcgt atcgcctata attgttgtgt
     5521 gctctttttt cgtgcacttt aaaaaccctt taaaatcaac actttaaggg gtttttgttt
     5581 gtcttgtata agaaaaaggg gcagacgagg ggcacaattt aaaattttac cttgtctaac
     5641 ttgctagata tgtctgatac cattttttga gtaacgtgag aataaatctc tagtgtggtc
     5701 tttgagtcac tatgccctac tctgtccatg atggcagtca agggaatgcc cagctcagca
     5761 agtagggata tgtgagaatg tctaaatgta tgtgtagtta tgtttttttc tatgccgatt
     5821 ttttgaccat gtcttttcaa tgcacaaata accctggcat ttgttattgg ttctcctaga
     5881 gtattgatga aaataaaatc tgtatcaaat ccatttgtcg cattctctat tatttgctct
     5941 ttgataatat ctaacacttt ttgaggtgct gttataaccc tatcggactt gattgtcttt
     6001 ggtgtagttc tctctttttg tctgaaatcg tatgtatgct tgatgtgaat agtcttttta
     6061 gaaaaatcta tatcttcctt gtagtttaag gctgccagct ctccatacct catgccagta
     6121 agaaaaagaa ctttagctat tcggatatac tttgtaattc gataatcaca tagggcctcg
     6181 tcttttaaat tttggatgaa taacttaaac tctttttggt ctaagtattt tgtatttttc
     6241 tttctgagtt cgtcggatgt aattactttt ctaggcgttt caacaaatag catttcattt
     6301 gtatcaatat aattcattct gatagcgaat ttcattatct gattgagctt gaacttgatt
     6361 ttagaaacat agttatgaga tctcccatct tgtaatagtt gatctattac tttttgtaat
     6421 aaacgtctat caatatttct aactaggtag tcgccctcta tctgctttaa aatctctttt
     6481 tttacatttt ttgaagcata gactgttgaa tttttaacac catgtttcca attttcctca
     6541 aattcctcat atagtttttc aaaagttata ttagaaacag aatgttgttt ttcccctaac
     6601 ttttgtttta tcttttcctg caacaagatg gcggcttgat ttcttgcttg tggagttttc
     6661 ttctccatgg ttactgaaac tttttttaat ttctcagtat aagggtcttt gtatcgctca
     6721 aaatatttaa acttgccatt tggcaattct tccatccaca ttgattttac ctcacttttt
     6781 tgataaaatg ggtataagaa aacgaccttt tgaatggttg tttcctatac atgatttcct
     6841 cacactcaag atttggcgat gacgagtgtg gggatttttt tatttcgttt caaagaaatt
     6901 tccgcaattt tggcaatgcc actgtttatt tccttttttg ccagcaaatc cagctaaagc
     6961 accaactcca ccagtcaaaa cagctccacc aacggccttt ccaacagaaa aggctttttt
     7021 gtcttgttgt aagaaagtca catctttaga cttacaatta gggcaagtga tgatattctg
     7081 ctttttctct tgctttttct tctcttgatt tgctctaaat gtttcaagtt cacgttctct
     7141 ctgctctgga gatttttttt ggttatcaat tactgcacct atgacacctc caacaaaaat
     7201 aacaattatg ataatagcaa ttatattcat tttctctcct tttttaatta agtagtgcta
     7261 aatattcttc tttgaccatg atttcatcgg caatggtttt taaattatac ttttccataa
     7321 ataccaggta attaaatgta gtggcatctt cggctatatc caactcagct ttcataaggt
     7381 gatggatcat attcctatta gcctcaagct cacacttttc cctaaaaagt tgataagtat
     7441 ctggcgcgtg gtttctatgg cctatctcat gtaaagcgac ttgtaccctt ttttcatcag
     7501 atatagcatc actcaaaaac atagttttga gggctgggat gtaaaaggct tcatctggaa
     7561 atagaccatc ttcaaaaatc tctatatcta tacctagatt ttgagaaaat tctttttcag
     7621 tcataaacaa tccaaccttt tattattatt gctttctgag ataaatctct attatgtttt
     7681 gaattgcttt tttatcttct tcagttagtg gtttaccatt gaagcgcata gcggtagagg
     7741 caagttcctc aacatcgact tctttgcctt cgaaaaagaa ttgttctttt ttattagcga
     7801 tggtaggatt atctgtgcga ccaagtaagt agtctgtaga tacgttgaag tagtcagcga
     7861 ttttttcaat tttatcgccg ctaggagttg aagtttccca ttttctgaga ctgccattac
     7921 taaaatttaa attcctttcc aattcggcaa gagtaacctt tctatcattg gctaacgagc
     7981 gtattctatc taaaatagtc atgtgtaaaa acctccaaaa ataaggcttt acaaaataat
     8041 gtaaaatttt ctactaaaat gattgacaga tagaaaattt tccgttatac ttattttgta
     8101 agctagttga ccagctgaca taaatacaaa tagaataatc cgccaagatt ttcgttatat
     8161 ctgtttttat gatatagctg tattttttat acccttataa tagactattt tctattattt
     8221 gtcaatgaat aatgcttatt ttcttataaa attttctatc aaaaggaggt gccggaagtg
     8281 atttatgaca aaataaaaga aattgcttca gagaagggga tttcgattta tagaatcgaa
     8341 aaagatcttg atttaggtaa cggggcaatc agcaaatgga acaacagttc gccatctgcc
     8401 actactctaa attcaattgc aaattattta aatgttcgtc ttgaacaatt gctggaggaa
     8461 taacatgaac gaactcatca acgtaaccct gaatgacaat caggagccag tagtgtcagg
     8521 aagacaacta catgaggcgc tgggtgttaa ttcaagatat acaacatggt ttgaccgtat
     8581 gaaggaatac ggatttacag aaggtcagga ctttctccca aatttgggaa aaagtacagg
     8641 agggcgacaa gctactgacc acatcatcaa gctagacatg gccaaggaaa ttgctatgat
     8701 ccagcggacg gagagaggca agcaagtccg acaatacttt atccaagtag aaaaagactt
     8761 taatagccct gagaagatta tggcaagagc attgctcatg gctgatcaga aagtccacaa
     8821 gctggaggct cagattgaag cggacaaacc caaagtcctc tttgcagacg cagtaagtgc
     8881 aagccataca tctatcttgg ttggcgaact tgccaagctc attagccaaa acggctacaa
     8941 aatcggtgcc aatcgcctct tttcttggat gcgcgaaaat ggctacctga ttaagcgcaa
     9001 aggctcagat tggaacatgc caacccaacg tagcatggac ttgaaactct ttgaaatcaa
     9061 ggaaacaaac gtgcaacacg cagatggaca tatcactgtg aacaagacac caaaggtcac
     9121 aggcaaagga caacagtatt ttatcgataa gttccttaat taggaatacc tgacaggtta
     9181 gaaagtagaa agaatgattg aaaataagtg aagagaaagg agactgtatg acagacttta
     9241 aaaatttaga ttgtcaattt atctttcaag aatgcgactg aaaattatac tgctgttagt
     9301 aatagtttta tcaacgatcc tgcgctggat tttacagcgg ttggcatcat gatggtagtg
     9361 ctggctaatc acccaaattg gcaagtctat ccggatgaga tagctaaaag aaaaggtgtt
     9421 aaccgaaaga caatcgataa gtatttcaaa atctttgaag aggctggata tttacgaaaa
     9481 ataagaaaaa aacctcctgg aaatggaggg agtcatatat tcagattctt ttcagatgta
     9541 aaaatatctg atttccaatt cgatattatg aaacagagat tgaacctatc tatcaaaagg
     9601 gcgtctatga attataattc tgacattcca aaaagtgaga tgtcagaaag tgagatgtca
     9661 gaaagtgaga tgtcagattt tgggcactaa taaatactaa ctaacaacaa gtattaaata
     9721 acaataaata ctaactaaca acaagtacta ctcttaataa ataaaagaga gtatacaaaa
     9781 aagtatctga gaaactcaga cactttctaa aaaaatctaa cttaattata gcatgaaagg
     9841 ggaaaaatgg aaacagttca aatcgtgaga attaaagatg tgatcatcga gaagatttct
     9901 gcaaacgatg aagaactaga gcgcatcttt ggatgttcaa aacggcaagc gggagatatg
     9961 aggcgagaga tgaaaaaatt gcctagtcag caaaaatacc ttagaaacga tggtcagctt
    10021 gtcacaatca aaggttttga tgcttatctg caatatcgag gcagtcaatc atggaagaaa
    10081 gaaatggcta aaaccgttaa gatgacacga tagcagaata ataactacta acaaataaca
    10141 aactcatact tatagataat aagggaatta cagagttttt aaaggaggaa agaaatatgc
    10201 caaattgggc agagggaact cttaaattaa gaggcagacg cgaaaacgtt gcatcagctt
    10261 taaaagaaat gctattagga gatgaaggcg caacgcttga agaagaatac gatggcactc
    10321 tactaagatt taaaaacgag tatgattatt tttatataaa cggtacaaga cgtgcgttta
    10381 tttctagtaa agatattgaa atttggttgg atgatgattt tgtgattatc gaacttgaag
    10441 atttcgaaca agcatgggca gcattagctg acaattacac agaaatttct agtaagtttg
    10501 atgttgatat taaaattttc acttttgaaa tgggtatgga atttacacag gaaattgaaa
    10561 tttcaaaagg tgaaatcatc aagaatattg taaacgaaaa ctttactaac tattcatggg
    10621 atgtgccttt tagtagactt ggaggataga taatatggct gatttaacat ttgcagaatt
    10681 acagcgaaaa atgcaaatcg aaaaacaaac gaaacaggga gtgaaatatc cgtttagaac
    10741 cgcagaggac ataaataata aatttaagtc tttggatagc ggttggagtg tatcatttcc
    10801 agaagatgac atcattcaaa aaggtgacaa actgtattat aaagcggtag ctgttgctaa
    10861 aagagaaagt gatggcacga ttgaaaaagc tattggatgg gctagagaag aagatgtacc
    10921 aatttttcac acacaaaaag gggatgtgaa acagatgcaa gatccacaat ggacaggtgc
    10981 ggttggttct tatgctagaa aatatgcttt acaaggttta tttgccattg gaggtgagga
    11041 tgttgatgag tatccagtag aagaaagcca agagcaagga cagaataatc agcaacagaa
    11101 accaaacaac cagcaagccc aaggacaaaa tcaagtaagg tatattgaca acattcagta
    11161 tcaagaaatt aacgacctta taaatgatat tgcaaaaatt aaagggatgc cgttcgatac
    11221 gcttgctaac tatgtactat ctgaaaaatt aaaaggttta caagattttc atagagtaca
    11281 agttggtgac tacgaggtat tgaaaaacta tttaactgaa caactagcaa aagcaaaagc
    11341 aaagagaggt aattaaacat ggtaaaagat gtaactaata gcttgacaga aatcaaggtg
    11401 gattttcaac ctgcagtaat taatgttgac cgtgaggcga tcgaggcaca agtagccgca
    11461 gccattgcac agtatagcgg tcgcgaggtt actgttgata attacaaaga agtttatgaa
    11521 gagcgaaccc gctttaataa gctgattggg ggcttagaca ctcaacgcaa agattttaac
    11581 cgacaaatca atgagccggc aaaagacttt gataagtggg tcaaagaaaa agtcatcaag
    11641 cctattgagg gcgtaacaga tgctatgtca gcaggactta atgcgattga tgaacatgaa
    11701 cgattgatgc gcgtggatgt cgtgcgtgct acatttgagg ataagtgtat ggtcgcaggg
    11761 attgaaaaat ccacattcgc tgacaaatac gatgagtaca gcctcaagaa atattttaaa
    11821 acaggcaagt atgagctgaa aaagacaaca cttgatgaaa tggatggctt agtactttca
    11881 gaatttgatg ccctggaaga atacaaggct aacaagcaag ctatccaaga gcaagctcaa
    11941 gagtacgatt tgccagctga tagctatatc agacatcttg aagatggtaa gagtcttgtt
    12001 gatatactca agatgatgaa aactgaccga gatgctgaga ttgcacgcaa agagcagaaa
    12061 gaggctcaag aaaaagcaaa agctgaacga cttgaagaaa ttgctcaatc ggccaagaaa
    12121 aatgctaatg cgaatatcaa ggcttacgat gccgaaacag gcgagatttt ggaacagggt
    12181 acaattacac cagaacctca aaacaatgcg cgagaggtgg caaaatttga gcctagcgag
    12241 cctttggtca aattagtacg tcttgaattg cacggtggtt tagaacagtg ggaaaataca
    12301 caagaatatt ttgaggataa ctttatcggt tttgaaactt tggaggatta agtagaataa
    12361 ggagtcagac ctatgagatg tttttatgtc agcggtaaaa ttgcagatct tgatttgggg
    12421 tcagaaatca atgcagaaaa ttcatttatg gccgctattg agtttgtgaa acgatatacc
    12481 ggcttattaa agtttggttc aaatgaaatc aaggtatcag aagtagagga ggtgcaaaat
    12541 gataaataac gttgttttag tagggcgact tacaagagat gccgaactga gatacacgca
    12601 atctaatatt gcggttgcta cgtttactct tgctgtaaac cgtccattta agaacgaggc
    12661 tggagagcgt gaggctgatt ttatcaattg cgttatctgg agacagtcag ctgaaaatct
    12721 tgttaattgg gctaaaaaag gttctcttat cggagttaca ggagtaattc aaacacgtag
    12781 ctatgataac cagcaaggtc aacgtgttta tgtcacagaa gttgttgcca gtaattttca
    12841 actgttggaa agtcgtaaca gtcagcaaaa taatcaaggt catcaagacc atcatggcgg
    12901 ttatcagcag cagggctaca gtaatcaagg cagttctttc caaaatggaa atagttacgg
    12961 gcaacaaggc agtttccttg aggggaacac aacaaatcta gttcctgatt tcacccgtga
    13021 taacaatcca tttggcagac ccacaaatcc attggatatt agtgatgatg atttaccgtt
    13081 ttagcgaaag gggatattct agttaagtta tgaaattctt agacttattt gctggcattg
    13141 gaggttttag gatcggcatg gaatcagccg ggcatgaatg tataggattt tgtgaaatag
    13201 acaaattcgc tagagctagt tataaagcta tacacgatac gaaaggagaa attgaattac
    13261 atgacattac aacagtatca gatgacacta ttcgaggaat cggacatgtt gatgttatct
    13321 gtggaggatt tccgtgccag gctttcagca ttgcaggaaa cagacgagga tttgaagata
    13381 caagaggaac tctcttcttt gaaatcgcaa ggttcgcatc tattctcaaa cccagatatc
    13441 tattccttga aaacgtcaag ggactcctca accacgacag aggaaatacc tttgaggtca
    13501 tcctctcagc gttggatgaa ctggggtatg atgtggaatg gcaagtgctt aacagcaaag
    13561 attttggagt cccccaaaat cgggaacgtg tgttcattat cggacattct agaaaaagag
    13621 gcaccagaag agtttttcct atcggaagag caaatagaga atttgatttt gaaccaaaaa
    13681 taaagattgt tggtaacact aaaaatccga acgggacaag tcaagggact gggagcgttg
    13741 tttacgactc aaacggttta gtcggtacgc tttgcgctag agattataaa gagccaaaac
    13801 aagtagctat accagtattg acacctgata gagtaaataa acgacagaat ggtagacgtt
    13861 taaaaaaaaa cggtgagcct atgtttacgc tgacggcaca agaccgtcat gggattttaa
    13921 ttaaagaagc aacaaaaaaa ggttatgcag aggctacagt tggtgatagt gtaaacctat
    13981 ctcatccaaa ctctaaaaca aggcgaggta gagttggtaa tcagatagca aatactctct
    14041 taactggaga gagtcaaggt gtggttgagc ctgattttag gattaggaag ctaacaccta
    14101 gagaatgctg gaggttacaa ggttttcctg attgggcttt tgataaggcg caggaggtca
    14161 actctaacag tcaattatac aagcaagcag gcaatagcgt aacagtaaac gttatttctg
    14221 caatagcaca gggtttagga ggaaattaac cctatttaaa ccaatttgaa aaggaaacag
    14281 aaaatgacaa aaattgaaat cgttatggta cttacaactt tgatgtctat cacatgggca
    14341 gcgattgtta caattcacac tatgcaagct atcaaaaagc acaaggcaaa agtggattat
    14401 tatcagaaac cacaagtgca atgtgagatt gcacgtcatg tacttaaaaa caaatggtac
    14461 tcagatggtg gggaggtgtt tagatgaaag tatttgatgg cgctatttca aattagctta
    14521 atttaaagca ggaggacaat atggataaaa aacttattgg gttagatcta acccacattg
    14581 cagatggagg attacaggag aaactagaca aagagcttga aaaagtcttt gataacatcc
    14641 ttgacctaaa tacagatgcg aaagcaaaac gaaaagtgac tatcacactt acaatgtcag
    14701 caaatgaaga gcgtacagtt gttgatacta ccatggaggt gaaatcaaaa tttgcgcctc
    14761 aaaatggagt agctacaaca attcttattg ggcgtgattt tgatacaggg caagtacatg
    14821 ctaatgagct aaaaagtaca gtacctggtc aaatgtactt tgatgaaaac ggagaaattc
    14881 tgacggatat tgggcaacca gtggcagaaa ttgaacaaca agcagaaaca aaaccagata
    14941 ttattgattt caacaaaaag aaagtaggta actaatatga caacagaaaa tcttaaagca
    15001 gcattggaat acgcagtaga actaaatgag catggtttgg aaattttaac agctgcagat
    15061 ggaacagagt attatgatgc caacaaattc aacctcaaag aacttgaccc taaacgctat
    15121 cctaaaactc tggagctatc aaccttgaca agccttgttg actatctcaa aacagaccta
    15181 aacaatttga aaaaccaacg cttgattgta gcagttgaga aaaatgatga ggtttgtgtg
    15241 tggtctgaaa atgatgagtt agaacgtcgc acattacttg ttgatgttaa ggcacgcatc
    15301 ccagagttat cttttggccg tttcctatca tcggaacagt tcaatatcat gttgcaatca
    15361 aactttattg acgataacga tcgtggcaca ttgctagaat ttgctagcgc attgaaaatt
    15421 gagaatgggg ctgaaattga agataatgga gtatctcaag tagcaacagt taaaacaggg
    15481 gtggcaagcc ttgctaaagg caaagcacct aatccggtta cattgcgccc atatcgtaca
    15541 tttagcgagg ttgagcaacc ggcaagccta tttgtcttta ggattgataa gcaagccaat
    15601 atggctttat ttgaggcaga tggtaagcgt tgggtagctg atgcagtagg aaacattgca
    15661 gcctatctaa aagagcagct agcagaccaa aaacttatca cagtattagc ataagaaagg
    15721 ggaaattaaa atgagtgatt acaaacaacg gatgattgaa gaatacaaac aattaaaaga
    15781 gcgcaccaat aagttaagtt tgatgattag taactattac gtaggaacac ttgattttaa
    15841 actaaaatgt cctattgagt tacttgaaac tcaacactat acaatgtgtg catatctcaa
    15901 gatccttgaa cagcgtgcag aaattgaaaa cattgagttt taaggtaatc aaaatgaaat
    15961 ttgagttttc tttgcctcgg aatactaagc taaaatctct aaacatggtt atcaatagta
    16021 atgacagaca acatcaaaca gataaggcta aagttactaa gcgcattaga gcttttgctt
    16081 attggcatac atcaatgaac aaggataaag ggagggctgc ttttagcccc tctaaccctt
    16141 gtgaggttac agttacaatt tacagcccta ctaagtctaa attagatcca cctaacttgt
    16201 atccgacagt caaggctatc attgatggca tgactgatgc aggtatttgg acagatgata
    16261 atcataaggt tatcaaaaag ttatcctttg tttatggtgg cttaagtgag gaaaaagggc
    16321 attatagatt agagtttgat atagaggagg tttaaagtga ttgaagttaa tataaaattc
    16381 gataattttg aagcgcatgg cttttaccaa gatgatacta aactaggaaa aattagagat
    16441 gcaatcatat ctcaaatgaa taatgggcat gtggttattt taggggaaga tagaagtatt
    16501 ctattaaatc ctaaagttat taaatgtgta caatttgagg ttgtagaaga tgaccagatc
    16561 taatcgtttt ttgaccttta gagaatctga gttattttga ggaggtggag tgatggaaga
    16621 gttaaagaaa aaagttaatg aagtatacaa ctggacggta gaagacggga agccgcaacc
    16681 tcccaagcaa gatttaccac aagcggtgaa agaccgagcg gactattttt gggaaatggc
    16741 agaagatggt atgacgttta tgggagcgat ggaatgcatc ttcgctgatg aaaagcctac
    16801 agactatgat ttgggagcta ctaaggattg gttgccaaaa tctaaggagt ttgatgattg
    16861 gattggctat gcgccaagca tggctcaggt agttattgca gtttatttga tttatagagg
    16921 aaactaagat gaatattaag gcattgatta agaagtatga agaattgtgg aatgaacaca
    16981 gcccttttta tgaacctgta ccttatactt caatggttga actttttttg aaagagttga
    17041 aacaactaga cgaaccagaa aaagtcaaag ttccgcagtt tatggcggat tggattgatt
    17101 atttcaaaaa aaatggtgac tgggatttgt ttcaagcgat ggattatttg tttggaaaaa
    17161 aagaaataag ggaatggctt gagtataaaa acaaccaaga cctcttcgct cgtgcttggc
    17221 ttgacggcta cgaggtcgag aaagagaagc ggtatttggt gactttaaaa aataggcagc
    17281 ctttggtcaa atcgcaatca gggagtactc tttattttag tcaagatata acagctagga
    17341 attataaagg tactcaaaaa gaactagaag acgcaaagtt cggctgggta tttgattgcc
    17401 cagggattga gattgaggag gtgaagtaat gacacaaaca cttgaagaag ggatgaagaa
    17461 tcaaagtaaa tgcataaaaa tacctatgga aatcagaccg tttgatatag ggtatcgaat
    17521 agtgaatcaa catggaaatc cgcttgcttt aaaaaacgga gcaagtatat tcgatttacc
    17581 ttcgctagct gaaaaagcta ttaagaaaga gtttagtaaa gatgacccag attttgatat
    17641 tgaaaaacat ttcgttgaag aggttgctat tgtcaattta agtaaatttc atagttattt
    17701 tgaggaggtg atacatgact gatgacgaag aaaaaaatag agcgcttgtc agttatccat
    17761 cgcagggaaa tcaattggct aaagtggtat tttttgagag ataagaaaaa tccgaagaga
    17821 acaattctgg agcaaaaaat tatagtttct catatcaaaa ctgataggct tgaagctaag
    17881 tttttaacta acttaaaaaa atcaactgaa gattttatag atgggtctga tcctaaatat
    17941 ttgcgggcga taaaagaggt ttatgtttac gaaaacatga atgtcattgg agcttgtcaa
    18001 aaaatactat tttatagtcc gactcaagcc tatgtattac ttaatgcgtg gtttaacgat
    18061 tattttcgtg cgacttacac agaattacta gaaaacgcca tcttagataa ataaccgtaa
    18121 aaaatccaaa gcttatgtat ctataatcaa gatatataag ctttttcgaa aggagagata
    18181 tggagatgtt acagattgaa tatgtagata taaaatccat taaaccatat cacaaaaacg
    18241 ctaggcataa tgacggagag gcaacagaga aagttgctgc atccataaaa gcttttggtt
    18301 ttcagcaacc tatcttagta gatgataata acatcattat tacaggacat actaggctaa
    18361 aggctgctct ttctttaggt atagacacaa tacctatcgc tcacgctgta aacctcacag
    18421 atgagcagat aaaagcttat agactagcag ataatcgagt tgctgagtat tcaacgtggg
    18481 actcagaatt attaaatatt gagctttctc aatttgaaac aatagatatg gctcagtttg
    18541 gttttgagtt atcagttaca gggttcaact ttggtaacga ggaggagcag caagaggaaa
    18601 ctgaaaacga ggaggaagat gctgaggatt ttcatagaga taccacaata aaccaataca
    18661 acctttttaa ttatgacgat acaagggttg agggttttta taacatgcct aaaatcgagg
    18721 gcgtggatca tattcctaaa gattttcaag gctttaatta tgttttaaat aaaccagatt
    18781 acagctcatg cgtgcatttt ttcctagatg attatcaatt tgaaagaata tggcaaagac
    18841 cagactttta cattgaaaag ctgctagaat ttgatagcgc cttagctccg gattttagct
    18901 tatatcttga tatgcctatc gctatgcaag tatggaacat ttacaggtca aggctgatag
    18961 gtcagattat gcaagattac ggccttacag ttatccctac tgtatcgtgg gctagtgagg
    19021 aaagctttga tttttgtttt gacggcttgc ctaaaaactc aacgctagca atcagtacaa
    19081 taggcgtaaa gcaaaacaaa gagcagtttg aggtatggaa aaatggagtt actgagatga
    19141 taaaaaggtt gactccaaaa agaattgtag tatatggcgg aaaagtggaa tacgattata
    19201 aagatataga ggttgtatat tttgaaaatg caacaacgga aaggatgaaa gaaagtggca
    19261 caaaaactaa ctaaactaaa agatattttt aaacatgttt caagtattga tctaggtaaa
    19321 gagattttat ttgaagatct tgagctttac aataaagaaa cagaaacaag caaacaatac
    19381 caatctatcg aggaggcaga aaatgaccta tctttgatgg aaaaagtaaa taaaatcaat
    19441 ttcactctag gcggtggacg tggtgcaaat tttgagaaag ggaaagacgg taagtatccg
    19501 ggttttagag gtgctggtgg tgcaagagat agtgggagct caaaagcctt acatccagca
    19561 tctttaaaca atcaagggcg cttttcaagt gttgagggag ctattcaggg atttattaaa
    19621 aaacacggtg gctctagaac agaatacagt acagcagttg actctcaagg ctttgctcat
    19681 aattatgtac acggtgggaa aaacagcgta caaattttgc ctatctctgg tggatttaca
    19741 gcaatacata accatccgaa tggcagcaat ttctcaagta cagatttaca tagctttgca
    19801 gcattaaaag gtatgaatac actagttgca acgaatagct ctaaagcgta tcgaattaca
    19861 aaaggggcta actttgatgc taaaggcttt gataaagctg tgagcaagtc acgttttact
    19921 acaaaagatt acaataaagg agctgaccta tggctcaaga aaaacgctaa gaaatacggg
    19981 tacacttact catacgagta aaagaaaaga ggctaaggta tgggtggtag aggagcaaaa
    20041 ttaaacttgt caacaacgaa aatgacaact caaaatatag agacttgatt aacaaaatcg
    20101 aaaagttatt aggaatagcg taaaacatcc ccttttttaa catatacaat gaaatcataa
    20161 gtataaaatg cttgtgattt ttttgtttga aaggagggtg gaaattgcct agagatggaa
    20221 ctaaaaattt aaaaccagtt acagaacgaa ccaaagatga agcaagagct attagctcaa
    20281 aaggaggtaa agcatctggc atagcaagaa gaaaaaaagc tgatctcaaa aaagcatttg
    20341 aaaccctctt atctttggat gtgacggata gtaaaatcaa gaaacaactt gaggagatgg
    20401 gtatggctgg caataacgag gctttgctag cctttgcaac ctttcagcaa gctgtaaaag
    20461 gcaatcagaa agcgactgag aacataatca agctgacaaa tactaaagat aaatacgata
    20521 tacaagaaca gaaagagcgt attaaagcac tcaaatatga aaatagagag cgtgctgaag
    20581 ctgagaaagg ctcaagtgaa actatcgaga tagtggatgc atgggctgaa gatgtgaggg
    20641 gggcaacgga tgacctttaa tgtccagaag aacatcaacc ctcatttcaa atctgtttgg
    20701 atttctagct taccttataa tgttttgaaa ggtgggcgta actcttttaa atcatcggtt
    20761 attgtactta aactagcgta tatgatgata aggtatatta tcgctggaga ggcggccaat
    20821 atcgttgtta tccgtaaggt ggccaatact attagagaca gtgttttcaa taaagtttgg
    20881 tgggcattga acctttttgg tatagctgag cagttcacaa aaacagttag cccgtttaaa
    20941 atcgtacaca aaacgaccgg ctcaacattt tacttttacg gacaagatga ctttcaaaaa
    21001 ctcaaatcaa acgacattgg aaatatcata gcggtttggt atgaagaggc tgctgaattt
    21061 aatgaccaag aggactttga ccaatcaaac gtgacgttca tgaggcagaa acacccacgc
    21121 gccaagtttg tacaattctt ttggagttac aatccaccta gaaatccata tagttggatc
    21181 aatgaatggt ttgagagcat caaaacgaat aagaactatt tagctcactc aagcacctat
    21241 cttgatgatg aactcggatt cgttactgag cagatgctag aagatataga gcgtatcaaa
    21301 gagaatgact acgattatta cagatactta tatctaggtg aggcagtggg attaggtaac
    21361 aacgtgtata acatgagtat gtttcatgct attgatgctt tgcctagcga tgataagctg
    21421 attggcatat catttgcgct agatggcgga catcaacagt cagcaacagc ttgttgtgct
    21481 tttgggataa cagctaaagg aaaagtaatc ttacttgata cctggtacta ctcacctgct
    21541 ggccaagtgg tcaagaaagc acctagtcag ctatccaaag agatatatac ttatatacgc
    21601 tcagttattg agaagtacag agtacaagcc ttgcaataca caatagatag tgctgaggga
    21661 gcattgagaa accagatgtt tcttgacttt ggtttgaaat ggcatccagt cgctaaactt
    21721 agaaaagtga ctatgattga cagttttcaa tctttgcttg ctcaaggtcg cttttactat
    21781 ctcaacaccg aaaacaacaa gatatttatt gaagaacaca agatgtatcg ttgggatgaa
    21841 aagactatca aatctgataa tcctagcgtt atcaaagaag atgaccatac atgcgacaca
    21901 acacagtatt ttgtgttaga caatgcaaaa ttgctcggtt tgcgtgttgg taacgtttag
    21961 aggaggacga tcatgagcct atttcaaaaa gtaaaagact tttttagtcg agggaggtat
    22021 tacatgcaga catcaaacct taatagtatt ttggaacatc caaaaattgc agtgactcaa
    22081 gaggagtatg accggattaa gagaaatcta gtctactatc aatcaaaatg ggatgatgtt
    22141 cagtacaaga atacggatgg agatattaaa tcccgtccaa tgaatcactt gccaattgca
    22201 agaacagcat cgaagaagat tgctagctta gtttacaatg aacaggcaac tatcacaaca
    22261 aaaaacgaaa ttttacagaa atttttggat gacatgctaa ctaacgaccg attcaataag
    22321 aattttgagc ggtatctaga aagctgtttg gcacttggtg gcctagctat gcgcccttat
    22381 attgatgggg ataaggtcag agtggcattt attcaagcgc ctgtgttctt tccactagaa
    22441 agcaacacac aagatgtttc aagtgctgca atccttacta agactatcaa atctgagggg
    22501 cgtaagaacg tttactatac ccttgttgaa tttcacgaat gggtaacagc agacggacaa
    22561 gaaacaggta gcacaaacga taaaaagtat tatcgtatta caaatgaact ttataggtca
    22621 gatgtgaatg atgtgttagg tcaacgtgtg aacttgagtg aactagacaa gtacaaaaat
    22681 ttagagcctg taacagtctt tgaaaaccta tcaagacctc tatttactta tctaaaaact
    22741 ccaggtatga ataacaaaga catcaacagc cctcttggat tgtctatctt tgataacgca
    22801 aagacgacta ttgacttcat caatcgttct tacgatgaat ttatgtggga agtgaggatg
    22861 ggacaaagac gagttattgt gcccgaacat ctaacacaaa gacaatatca acgtccagat
    22921 ggaacaatag attttagacc acggtttgat gttgagcaga atgtttatat gcaaattggc
    22981 ggctctagta tggatgccgg gggcattaca gaccttacct caccaattcg agcaaatgat
    23041 tatattttgg cgatttcaga gggattgaaa ctctttgaaa tgcagattgg tgtatcaagt
    23101 ggcatgttta cattcgatgg tcaaggaatg aaaactgcaa cagaaattgt cagcgagaac
    23161 tcagacactt atcagatgcg aagtagtatt gtcgcacttg ttgaacaatc tatcaaggag
    23221 ctttgtgttt caatgtgtga gctcggtaag gcggtagggg tttatagcgg agaaattcca
    23281 gaacttgatg atatttcagt taatttggat gatggtgtat ttactgatag gcatgcagaa
    23341 cttgattatt gggctaaaat ggtagccgca ggattctcaa ccaaaaaacg ggcaattggt
    23401 aagacattaa atatttctgg tgttgaggca gaaaaagaac tcaatgctat caatagtgag
    23461 ttgctaccta tgaatgatgc cgaacttgct atttatggta tgcatgacca aaacgaggag
    23521 gaagcagatg acaaaggtta aattcggagt tactagtgtt gactactcag caagcattga
    23581 agatacgcca acaataaaac taggtttaat aattagaggg agcgggagac tagatgcctc
    23641 ttcagttatt aaaaaactaa tcaaggatgt ttctgaacta gaatacgaat tagaagaata
    23701 aactggtcaa ttggccaatt ttctttcaag agagggcttt tgaatgaaaa aaaagagaaa
    23761 acagatcacg tttaacgacc aacaatttcc tttgcaaatg caaggcgttg gggatattta
    23821 cgaaaaatta cagattgata tctttgaccg tatgataaaa cgcttaaaag agcgtgggtc
    23881 tattgattta atgagaaacc cttatatctg gcagttagag aaactaaatg atatgcacat
    23941 gctcaatgaa cagaatctaa agcttatttc agagcgtaca ggaattgctg aaagattgtt
    24001 gcgtgatgta attgagaatg aaggtttgaa agtctataag gacactaaac agcaacttga
    24061 agaagatttg aataaaatac ctgagggaga gatttcaaat ggcgtaacgg acagcttaga
    24121 ggcttattcg aggcaagcag ttagtgattt aaaccttatc aatacaacat tacctaagag
    24181 cttgcaagta gcttataaat cgattgtgga ggagacagtc gcacaggtag ttgcaggaac
    24241 taaaacaagc gatgttgctt tgcatgatac catcatgaaa tggcagaaga acgcttttac
    24301 gggctttgtc gataaaggtg ggaggcattg gaaagctgat agctatgcga gggctattat
    24361 caagagcaca acatacaaag tttataacga aatgcgtact agacctgctg aggagttagg
    24421 agtagatact ttttactatt cgatgaaagc aatggctaga ccagcttgta gtccgttaca
    24481 agggcagata gttacaaaag gggctggtag gaagatagac gggataacta tctattcatt
    24541 attggattat gggtatggaa cagcagcagg atgtttagga atccattgtg gtcattatct
    24601 gacaccgttt attgttggag ttcatgagtt accgaactta ccagactacc tgaagaatct
    24661 aacaccagaa caagctgaag aaaatgcacg cattgaagca ggtcaaagag gccttgagag
    24721 acttatcaag acacataaag agcggttgca ttacgctcat accttgcaag atgacaagat
    24781 gatacaagct gagcgtttga aagttagagg gtatcaaact aagatccgta acttgataaa
    24841 tcagcatgat ttcttaacaa gagattacag acgagagaaa ttatatattt cataaaggat
    24901 ttgtgtttca caagtccttt tttgtgttta aaaccgtaaa aaatccctat ccatcaaagg
    24961 tatattgaga gagtaaataa tattttgctt taggtgggag ttatccacct aaaaaagaac
    25021 taggagggta caaatggcat ttacaactga agaactactc aatcttgggt tgacagaaga
    25081 acaggctaag tcagtctttg ctttgcgagg aaaagagctg aatgaggaca aatcagcctt
    25141 agaaactatc aaacaagagc gagatagtct caaatcacag ttgcaaaaag cagaggagca
    25201 agttgaacac ttgaaatcgc ttgagaatat cagcgctgaa caaaaagatg cgattgataa
    25261 attgcaagct gaatatgaca agtataaaaa cgaagctgca gctgaacttg cacagacaaa
    25321 aaaggttagt gctatcagtc tagctctgaa agatacaaat gctttcaatc cagacaaatt
    25381 gatgaaattc attgatgttg atgctatcca gttagacgac aacgggaaac ctcagattga
    25441 tgaagtaatc aacggtttaa aagaaagtga tccatatctg ttcaaagctg aagaaagtaa
    25501 gcctagcccc aatattttac ctcaaggtaa tccggcagga gagggtacaa gtgatgttga
    25561 tccgttccaa gcgattattg acgggtatgg caaataacag aaaggagatt acaaatgcca
    25621 agtaatcaaa acaacgcagt gcgccgctat gagaaacaat atgcgggcat tcttgagaca
    25681 gtttttagag tgcgggcagc attttcaaac gctctagcat ctattcagat tttggatggg
    25741 gtacaagaaa actctaaggc tttctcagtt aaaacaaaca acacaccagt tgtaatcggt
    25801 gagtacaaaa ccggtgaaaa tgatggtgat tttggtgata actcaggggc tcagtcacgc
    25861 tttggtggtt tgacagaagt taaatatgaa aatacagatg tcaactatga ctataccctt
    25921 acaatccatg aggggcttga ccgttacaca gtaaacaatg atcttaacgc tgctgttgcc
    25981 gaccgtttga agttgcaatc cgaggcacaa actcgaacag tgaacaagcg aattggtaaa
    26041 tacttgtctg acaacgctac taatacggaa gcccttgctg attttacaga tgacaaagta
    26101 aaagctttat tcaataagtt gtcggctttt tacacaaaca acgaagttac agcgccgatt
    26161 actgtttact tgcgttctga attttacaac gccatcgtag atatggcatc agttacaagc
    26221 gccaaagggg caactatctc ccttgatgaa aacgggctac caaaatataa gggctttacc
    26281 ttggaagaaa cgccagcgca gtactttgag acaggagtta tcgctatctt ctcaccaaac
    26341 ggtattgtca ttccgtttgt tggtatctca acagcccgtg taattgaggc tgaaaacttt
    26401 gatggtgtga aattgcaagc tgctgctaag ggtggtactt acactcttga tgacaataag
    26461 aaagcaattt acaaagttac aggaactatt gtataggagg tagaacatgg caatttacaa
    26521 agcaacaaaa aatcttttct ttgagcaact caacatggat gtgattgtca atgacattat
    26581 tgaacttgat gaagattatg ctaaagaagt caacaagaaa ctaaaaaatg cttttccaga
    26641 tgtgaaaaat gttttagaac ttgttgacaa aaatggaacg cttgaaccag aagatgcccc
    26701 atctgtagat gatgcatctc aggcaactgt tgaagattaa ataaggggtg gcaacaccct
    26761 ttatttttaa gggaggttac acatgactta tttgacacaa gaggagttcg atgagttaga
    26821 ttttgatgaa gttacagact ttgaaaaatt ggcaaaacgg gcaaagatag cgattgacct
    26881 atacactaac ggtatttatc agaaagacat tgattttgaa aaagaaattg cctatcgcaa
    26941 atctgctgta aagcttgcta tggcattcca aatagcctat ctcgatgcct ctggtattat
    27001 gtcagccgat gacaaacagc tagccaatag tgtctctatt ggccgtacat caatctctta
    27061 tagcacctca caaagcacat tagcaggtca gcgatttaat ttgtctatgg atgctgaaaa
    27121 tgctttgaga caagccggct ttagcctagt tgttggagtt gcctatgatc gataagcggt
    27181 tattaaaagg gattgacaag cgtttgttaa aggatgtgct aaccataaaa aaagtagctg
    27241 ataaaaacga ttatggggat gaagtatatt cagaaccgtt gactattaaa aatgtacgtt
    27301 ttgatagatc agtgggggga tctggtaatc gtaattcaaa aactggtaca ggaaattcaa
    27361 aatcaaggca aaaacaaggg gttatatacc tctatccctc gctatctttt gtgacagttg
    27421 ataacagttg gatgggtgca aaagtaaacg atgggatagg agattacaca attaatggat
    27481 ttcaaactaa ctattatgat ggtgagatat tcagtcaaga aattgaggtg atctaatgaa
    27541 tatcgccatt aaagttgact tgcagaaagc taaacagaaa ctttcgaacg aatccatgac
    27601 aagaggaaag attgcagtcg ctagcaaaat cttgctagac aatgagcaat atatcccctt
    27661 gaggggtgga gagttgagag cttctggccg aatcgttgga caaggtgatg ctgttgtcta
    27721 tggaacagtt tatgctagag cgcaatttta cggagcaaac ggcattgtca cctttaggag
    27781 atataccact ccaggtacag gaaaacgatg ggatcaagtt gctactagta aacatgctga
    27841 agaatgggct agagcttttg tgaaaggaat ggggctttga tgcgagagaa tgactttcaa
    27901 aatgtacttt taaagcatat caagacttta aatttaccag ttgaaccacg ctttgattac
    27961 tttgaggatg acaaagatga tctggttatc aatcagatac caggcgggaa agtggataga
    28021 gagtatatgg atggcacaca agaagtttct ttgccgtttg aaatcgccgt aaaggcaaaa
    28081 aagaactcag tagccaatga cactatttgg ttagtcacct cagaactagc aaagatagac
    28141 ttagttttac caagtgacaa taattcttat gaatatatgg gaatggaagt cagcaggcct
    28201 gccatgaaag gcaaggatga gcaaggctat tattattaca caattgaaat tgtggcgaaa
    28261 atcgtaatag agaggaacaa acaatgacaa gacaaaaaaa cgccctacgt ggccattttg
    28321 tagctccata caatggagga actgaaccaa caacagaaga tacatggttg gaacttgcta
    28381 aatggatctc agacgtatca gatgatacag acgagaaaac agatgatcaa gcatactatg
    28441 acggtgatgg agttgaagaa acaaccgtgg tcagcgtaaa aggtgcttat acctttgagg
    28501 gtacttacga cccagacgat aaggcacagg ctcttattgc tgggatgaag tacaagacag
    28561 gggatgaccg taagctatgg cacaaggttg tttcttctga caagaagaaa caatgggtgg
    28621 gagctgcaac tgcaacagaa atcaaagcag gttctggtgc tgcctctgac tatgaggtgt
    28681 ttggatgtaa gctttcttac aactcaacgc caaaagagac tggtattggg taatagcttt
    28741 tgataagggc gggcattgag ccttgccctt tttaacaaca ggaaaaggag taaagacatg
    28801 acagatattc agattgaact aaaacgtaca ggatttccag taaaaatcgg agaagtagag
    28861 ctatggtttg atacaagtca agagagctta atgcgctttt atgacatgga agaagaacta
    28921 aaacgtcgcc ttgtccaata tgaattagat gtggtatctg caaatatcaa taacaaaatt
    28981 gagcgtgatg gagtaactaa agaagtagtt gctggggcta ttgaattgga gaagaaacag
    29041 cttgagattc aatatgatct tatttttggc gacggtacat ttgacaagtt atattctgta
    29101 tatccagatt ataacgccct aaataacgct ctagaacaga ccgcaatcat gttgcatgac
    29161 aagttggaag aacttgctga gcaacacaaa acggtggtga aagagcgtgc tagtcactat
    29221 ttaaacaagg gaaaagtcac tccaatcaag aacaacaaga aacaaaaaaa gaacaaaaag
    29281 aaatagcagg taaaaaatat gtctatgaaa ttaaatgatg ccttaatcac aaatttttct
    29341 attgctgata aagagtacga catagacctg tcttttaata aagttctaga tgtctttgaa
    29401 atcctaaaag aggatgaaat gacgcgtcta gaacaagctc agttgattgt ccatttgcta
    29461 actggccaag aattatacga catcaaagag gttgtagatt gttggattta cataaaagaa
    29521 cactttctag ggatcgaaaa agaaactgtt cagtatgatt tgctaggcaa tcccatgcca
    29581 aaggcaaaag gtgaagaaga acaagaaaaa ttgattgatt ttgaacaaga tgcagagtac
    29641 atttacgcta gttttttaca agcctacggc atcaatcttt tgaaagttca aaatgagttg
    29701 acatggacag aatttaaagc acttttgaac gctttgccag ataacacaat catgcaacag
    29761 attatagaaa tccgagcatg gaaaccagaa tatggtgggg ataagaataa aatgcgtaaa
    29821 ttacaagcta aatatagttt aggaaaggag ggagaagata atgactgatg gaaaagtgac
    29881 catcgttgtc gatgtggatg gtaataaagt caaggttcta aacgatgagt tagataaaac
    29941 ggcacagaaa ggtgacagag ggagcgattc tctaaagaag tttgcgattg gtggtgctgc
    30001 ttttaaactg gcatctaaag cggtagatct tctaacagat tccttgggcg gagcgattca
    30061 acgttttgat actcttgaaa gttatccaag agtgatgcaa gcgatggggc atagtacaga
    30121 agatgtcacg cgctcaacta agaaactagc ggcaggtatt gagggtttgc ctacgacttt
    30181 aaatgaagtg gtaggcacag ctcaacgcct tacctcgatt actggcgata taaacaaatc
    30241 aacagattta acacttgctc ttaataatgc ctttcttgcc tctggatctt ctagtgctga
    30301 tgcaagccgt ggtttacaac agttcagtca gatgttatca gctggtaagg ttgacatgca
    30361 aagttggaaa acgttacagg aaaccatgcc ttatgctttg caaaagactg ctgaatcatt
    30421 cggttttgct ggccaatctg ctcagaatga tttctattct gcattaaaag aagggcgtat
    30481 cactttcaac caattttcaa gcaaattggt tgaattgaat ggtggcgttg gtggttttgc
    30541 agaacttgct aaaactaaca gtaaagggat ccagacatct tttgggaact taaagaatgc
    30601 ggttgttaaa ggtgtagcta atactatcaa ggctcttgac gatttaacaa aggcagcaac
    30661 aggtaagacg attgctgaga acttcgatgc attgaaagta atcatcaatg cggcttttgg
    30721 tgttattgtc aacgtaatta aagctagcac acctgttttt cagactttgt ttagtatttt
    30781 gggtattgga gcttctgtaa tctcattttt gagaccggct attatcggtt tagttgctgc
    30841 tttggtaact atgcgtgcca tcaatcaagc ggtaaaaacg actaaggact tgataagcgc
    30901 gtggaaaata ttcaaactaa cagccacaag agcgattcgg atcatcaatc tactgacagc
    30961 tgcccaagct acttatggct caataacaaa ggctcaactg gttgctcact tggccaataa
    31021 cggagctttg acagcatcca atttgcttta tggggttcta actggctcta tcagcttaca
    31081 gactgctgct actattgctg cgactgctgc aactaccgca tttaaagcag gactgaccgc
    31141 agttaaagca gcgctgaccg ctttaactgg cccgattggt ttggttgttg ctggtgtagg
    31201 tcttgctgtt ggggcattgg tagggttgtg gcaatggcta actgccgaga gtgaggagac
    31261 caaacgcctc aaatcagaac aagaggagtt agtcaagagt acggatcaat taacggattc
    31321 tgttaaacaa agcgcaaaag aacgtcaaaa aaatcttgag tctgtaaaag gtaatacaga
    31381 atcttaccaa aaattggctg acgaaattgt ccagttatca caaaagacaa ataagacagc
    31441 agcagacaag aaaaatctca agaaaaagat tgatgcttta aatgcctctg ttagtggtct
    31501 aaatctagct tatgataaga actcagattc tttgtcacac aatagcgatg aaatcaaagc
    31561 tagaatctca gctatggagg cggaatcaac atgggagaca tcccagaaga acctgcttga
    31621 tatcgaacaa aagcgcgctg aaattggcga gcaactgaag cagatagctg aacaacgcaa
    31681 aaaatggaat gaagaatcca atgttagcga tggtgtccgc aaagaaagac tgcaagaact
    31741 caacgacaag gaaactgagc taaaaaatac tcagacagaa ttgcaaactg agtacgaaaa
    31801 aacgtctcaa gttcaacagg cggcatctga agcgatggct gctgctgccg aaaatggttc
    31861 aaatagacaa gttgtagcat acgaaaatat gtctaaatct caacaaaaag caatagacga
    31921 tatgcgtact aagtacaatg aattacttga gacaactacg aacatgtttg aacaaatcaa
    31981 gtataagtct gctattagtg tcgatgaaat gattgccaac ctccaaaaaa accaagaggc
    32041 ggttaataat tgggcaacaa acctcaatac attggccgaa cgtggggtaa acgaggggat
    32101 tttagctaaa ttacaagcga tggggcctca aggtgggttg tacgttcaag aactcgttaa
    32161 tgcatcagac gaaaaattgg caacattgaa cgaagtcttt actcaaggtg gtgagtcagc
    32221 tatgaatggc ttaactgctg gtatggatac gggtgctttg ggtatcacag acaaaatcaa
    32281 gggtattgta caaagtcaag tttcaagctt acaagaggaa attgcagctg ctgacttttc
    32341 tagtttgggg caagaaatcc ccaacggggt cagtcaaggg atagaacaag gagcttctac
    32401 tgccggagaa tcttctaaaa acatggctaa tgatataaaa gaatccttta cgagcgaaat
    32461 ggatattaat tccccatctc gtgttttcaa tgagtatggt ggttttatca ctactggttt
    32521 agctgagggg gtagataaag gtaccaatca acctgtatca tctgttacta atttagccaa
    32581 tcaaattaag aaaccatttg atagtctgca gagtgatttc acgtacattg gtgaaatggc
    32641 gatgtctggt cttaatgcag ggctttggag tggctctggt tctgttatgg caacagctaa
    32701 ttcaattgct gaaagggtaa aagcgaccat caagagcgca ttagatattc actcgccatc
    32761 tagagcaatg cgtgatgaag tcggacgttt cattcctcaa ggtatcgctg ttggtattga
    32821 agcagatgca ggggttgttg aaaaatcaat gttgcgatta aaagaaagca tgatgattga
    32881 tactagacca gaaattgcac ttggcttaaa caagaaacta ggtgctcaag tgactgttaa
    32941 acaaagtagt aagcagacaa tagctgaaaa aatcaaagtt actatggaca agtctagcga
    33001 actactcaaa aaagccctgg atgtagctga gacggccgtt agacgaccaa atgaaatgta
    33061 cttaaacgat ggtactttag tcgccagaac aggcgataaa tttgctaaat atcaatcgga
    33121 acaactaaga cgagataata ggatgaaagg ggtattgtca tgacaaagat aatgactttc
    33181 aacggagttg atatgtctaa attctttcgt ataacagata ttatccgccc tatcgggaac
    33241 aagaggagcg tatcaactga taacgctccc ttattgggcg tgaatatcca acaggttaag
    33301 attggagaaa aagagcatat cataaaattt gacatcaaaa ccacaaatgc aattgaaatg
    33361 gaacaattaa agcatgattt ggcaggcatt ctaaacgttt tagagccagt aaagattact
    33421 tatggcgatg agccagacaa atactatatg gggttgccgg tagatgagat tactccagaa
    33481 aatttgacaa gatggttcca gcgctcagag ttaaaaataa taattcctga tggcgtggct
    33541 cacagcacga ctttaaaaaa ttttgatatc gatacaaatg aaacaagcgc accggatagg
    33601 atagtattta atttaacaaa tacaggaaca gagccagctt atccaattat tagaattaaa
    33661 cacaactcag agaatggata tattggagtt gttaacaaca gagcggcgtt tgagttagga
    33721 aatcgtgaag aggctgatac tgaaaaatac agagactctg aaacattgat agattataga
    33781 gggactaata ttctaaaagg atttcaaaat ggcactaaag gagtagctgt aacaaatgat
    33841 aataaggagc gtcttgttgg cactttgagt acaacaagta tgtgggggcg taatcatatc
    33901 gaattatcaa accgtggtac agttgaaaaa aatcgaaata atgcacaaag tttgacatgg
    33961 gctattcctg ttgatagtag tggagaagtt ggttcattga atgactacct gctttggaga
    34021 caagttttta tggcagctgt cgctaatcaa tatggtttca taaaggttac tgtatcggat
    34081 acagacggca atttcttgta tggggtggaa acttacaaac gctatcagac acttgattgt
    34141 gagtatagtt ttttcaccac tgacggcaaa ggtggataca agtttatcaa atggtggtat
    34201 tttactggga caggggctca agtaggcaaa cttgatccat ttagcgcgga aaagggctgg
    34261 tcagagttaa aaagaaacga tgatagagtt caagtgtttt ttgatggctc tcattatgac
    34321 tttattattc cggagataaa agacaaaaaa tcagcaaaaa tccatatcac gcttggagca
    34381 ctcagagact ggcctcttgt atcacatatg tatgttgatg agttcatgta tagaaaagac
    34441 tttgtgacaa agagtagaga tattcctaat cgctatccaa taggttcaaa tgttgtaatc
    34501 aacagtgagg acgatagtgt gtatattgac gggatatcta aagtaagcga agttgtagac
    34561 ggttcacatt ggccagcaat tcctccagga aaatctcaac tagagttgta tttttcacgt
    34621 tttgttaaga aaaaaccaac tgtaacaatc gaatttgaag aaaggtggat ataagatgct
    34681 tttgacaatc catgatgcaa atttacaaaa ggtagcattt attgataacg aaaaacaagg
    34741 tacgttaaat tattacgatg atacttggac aagaagtctt gcaacaggtt cgtcaacgtt
    34801 tgagtttacg gtatttaaaa aggctgtaaa gtctgattta cctcttgcta aagcctatca
    34861 tcatttgaat gagcatgcat ttgtctcatt taagtacaag ggtaaaagct ttgtgtttaa
    34921 tatcattatt gttgaagaaa atgagcagac aatcaaatgt tattgtgaaa atctcaatct
    34981 tgagttaatc aatgagcttg cgaaccctta taaatctaac aaagcgatga ctttcaaaga
    35041 gtattgtgag gcgatggatc ttttaaatta tactcacctt tctattggta tcaatgaaat
    35101 atcagattat aagcgtactc tggaatggga ggggcaagaa accaaactag cccgtctatt
    35161 aagcctagcc aaacgatttg atgctgagat tgaatttgat acacagttaa atgctgatag
    35221 cactatcaaa aagtttagtg ttaatgttta tcatgaaaac gatgacaacc atcaaggggt
    35281 gggacgtgta agaaatgatg tcattgttaa atacggaaaa aacatccact ctattacaag
    35341 aaaagtggat aagactggta ttttcaatac aatcagaccg actggtaaaa tgccaacggt
    35401 tgaagaagaa ccgagcggag ataagggctc caaaagcgaa actgtaaaaa atgcagatgg
    35461 ttcaacgacg aaaaccacaa tctctacagc ctcagatggg actaagagca aaactattgt
    35521 ccacactaaa gttacaaagt tagcggacaa gacacggatc acaacgacca caacgactcg
    35581 ttctgatggt tccatagaac aaactgttac aaccagcaaa aaaggcggag catcaacgtc
    35641 tgaaacaaaa gtcttgaaaa aaccaaatcc aaaagaaaaa acaaatacaa ctgaggatgt
    35701 tttgacgatt gagggattgg atgaatggga agtaaagaac gagaaaggga tagttgaatt
    35761 ttatcaaaga gggcaagcac tgtatgcgcc tatttcaatg caactatatc cctcaacctt
    35821 tactcattca acaggggagc ttgaccagtg gacaagaaaa gattttcatt ttgaaacaga
    35881 tgagccaaac gagttaagac gtttaggtta tctcaaattg aaaaagtatt gttatccagc
    35941 tatcacttat gaagttgatg gctttgtcga tgctgatatt ggagatactg ttaaagtcca
    36001 tgatgacggt tttgcccctc tattgatgat tcaagcacgg gttactgatc aaaaaatcag
    36061 tttcacaaat ccagtgagaa ataagacaat atttgacaat ttcaaggcac ttgaaaacaa
    36121 actatcagct gatatccagt cagcctttga gagattgttt gaagctgcta aaccatatac
    36181 tatcaaattg tcaacggaca atggtgttat ctttaaaaat cagatcggcc agagtctagt
    36241 aaccccaacc ttatacaagg gaggaaaacc agtcgttgtt ggtgttactt ggcgatgggc
    36301 acttgatgga gaagtaacaa cagggatgac ttacttagtt agaggctcaa atgtaactga
    36361 tacagttact ctgacagttg cagcttacat tggaaataaa gaggttgctg ttgatgagat
    36421 atcgcttgtt aatgttgctg atggaaaact tggtacacct ggaactccag ggcgagatgg
    36481 ccgtactcct tatgtccata cagcatgggc taataatgca acaggaacag atggatttag
    36541 tcttgatagc tcaatcaata aactctatat tggtatttat acagactttg aaccaaacga
    36601 tagcacagac cctaaaaaat acaagtgggc taaagtaaaa ggagaaaagg gagaaaaagg
    36661 agataaagga gaaccgggac aacgtggttt agatggcttg caaggtgcaa gaggtgaaca
    36721 aggattacct ggtcgcaatg gtgcagatgg ccgtactcaa tacactcaca tagcttacag
    36781 caatagcgct gatggaacta aggatttttc tgtaagcgcc tctgatagag cttatatcgg
    36841 tatgtatgtt gattttaata gagctgatag caatactcca tctgattaca attggacact
    36901 tgtaaaagga tctgatggcg caaacggtgt ggcaggtaag gctggtacag atggtaggac
    36961 accatactta cacatagctt acgccacatc aaataatggt tcacaaggtt tttcaactac
    37021 tgacagtaca aataaaacgt atatcggaac atacacagat tacactcagt cagatagtac
    37081 agattacaga gtgtataagt ggacgttgat aaaaggggca gatggtactg gtatttctaa
    37141 tgtaactaat tattatttag ctactacagt ctcaacaggt atcacaagaa caagcgcagg
    37201 gtggacaact acgccacagc ctatcacatc agacaagcgt tatttatgga attatcgagt
    37261 tgagctatac acaaacggta caagtaagac aacagagcct actgttattg gtgtgcacgg
//
LOCUS       DAVLTM010000007        34302 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLTM010000007 DAVLTM010000000
VERSION     DAVLTM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.007"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            465..917
                     /locus_tag="VYN16_000301"
     CDS             465..917
                     /locus_tag="VYN16_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1402890.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            936..2273
                     /locus_tag="VYN16_000302"
     CDS             936..2273
                     /locus_tag="VYN16_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1402891.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIRGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2352..3236
                     /locus_tag="VYN16_000303"
     CDS             2352..3236
                     /locus_tag="VYN16_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1402892.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3236..4069
                     /locus_tag="VYN16_000304"
     CDS             3236..4069
                     /locus_tag="VYN16_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1402893.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4087..6180
                     /gene="nanB"
                     /locus_tag="VYN16_000305"
     CDS             4087..6180
                     /gene="nanB"
                     /locus_tag="VYN16_000305"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1402894.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6193..7296
                     /locus_tag="VYN16_000306"
     CDS             6193..7296
                     /locus_tag="VYN16_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1402895.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7470..8168
                     /locus_tag="VYN16_000307"
     CDS             7470..8168
                     /locus_tag="VYN16_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1402896.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8187..9704
                     /locus_tag="VYN16_000308"
     CDS             8187..9704
                     /locus_tag="VYN16_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1402897.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9829..11157
                     /locus_tag="VYN16_000309"
     CDS             9829..11157
                     /locus_tag="VYN16_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1402898.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11409..12275
                     /locus_tag="VYN16_000310"
     CDS             11409..12275
                     /locus_tag="VYN16_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1402899.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12291..13130
                     /locus_tag="VYN16_000311"
     CDS             12291..13130
                     /locus_tag="VYN16_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1402900.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13402..13854
                     /locus_tag="VYN16_000312"
     CDS             13402..13854
                     /locus_tag="VYN16_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1402901.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            13987..14295
                     /locus_tag="VYN16_000313"
     CDS             13987..14295
                     /locus_tag="VYN16_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402902.1"
                     /translation="MDGQGITVDEMAWIIRGIVNGLIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14305..14604
                     /locus_tag="VYN16_000314"
     CDS             14305..14604
                     /locus_tag="VYN16_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402903.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKKEKIDLFMVGTYESFTDKKENSLQRKMMEEQTWHKKE"
     gene            14586..15230
                     /locus_tag="VYN16_000315"
     CDS             14586..15230
                     /locus_tag="VYN16_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1402904.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15399..16316
                     /locus_tag="VYN16_000316"
     CDS             15399..16316
                     /locus_tag="VYN16_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1402905.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16334..17218
                     /locus_tag="VYN16_000317"
     CDS             16334..17218
                     /locus_tag="VYN16_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1402906.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17234..18085)
                     /locus_tag="VYN16_000318"
     CDS             complement(17234..18085)
                     /locus_tag="VYN16_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1402907.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18285..20342
                     /gene="pbp2b"
                     /locus_tag="VYN16_000319"
     CDS             18285..20342
                     /gene="pbp2b"
                     /locus_tag="VYN16_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1402908.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMAIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPINSWYTQAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVLTNNLESAMGKLRSTFAEYGLGASTGIDLPDESTGFI
                     PKEYNFANYITNAFGQFDNYTPMQLAQYVGTIANNGVRIAPHIVEGIYGNNEQGGLGN
                     LIQSVESKEMNKINISESDVSILQQGFYQVSHGGSALTTGRAFSNGASVSISGKTGTA
                     ESYVEGGQEANNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYNQHH
                     PMN"
     gene            20353..20949
                     /gene="recR"
                     /locus_tag="VYN16_000320"
     CDS             20353..20949
                     /gene="recR"
                     /locus_tag="VYN16_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1402909.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21125..22168
                     /locus_tag="VYN16_000321"
     CDS             21125..22168
                     /locus_tag="VYN16_000321"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1402910.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHDPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22252..23625
                     /locus_tag="VYN16_000322"
     CDS             22252..23625
                     /locus_tag="VYN16_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1402911.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGVHDQILLKGSNSMNLAKLVE
                     SLENETK"
     gene            23612..24223
                     /locus_tag="VYN16_000323"
     CDS             23612..24223
                     /locus_tag="VYN16_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1402912.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIAALQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24314..25018
                     /locus_tag="VYN16_000324"
     CDS             24314..25018
                     /locus_tag="VYN16_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1402913.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25237..26610
                     /gene="ftsA"
                     /locus_tag="VYN16_000325"
     CDS             25237..26610
                     /gene="ftsA"
                     /locus_tag="VYN16_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1402914.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26627..27886
                     /gene="ftsZ"
                     /locus_tag="VYN16_000326"
     CDS             26627..27886
                     /gene="ftsZ"
                     /locus_tag="VYN16_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1402915.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVSPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27891..28562
                     /locus_tag="VYN16_000327"
     CDS             27891..28562
                     /locus_tag="VYN16_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1402916.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28572..29111
                     /locus_tag="VYN16_000328"
     CDS             28572..29111
                     /locus_tag="VYN16_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1402917.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29111..29374
                     /locus_tag="VYN16_000329"
     CDS             29111..29374
                     /locus_tag="VYN16_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1402918.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29371..30156
                     /locus_tag="VYN16_000330"
     CDS             29371..30156
                     /locus_tag="VYN16_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1402919.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30165..30953
                     /gene="divIVA"
                     /locus_tag="VYN16_000331"
     CDS             30165..30953
                     /gene="divIVA"
                     /locus_tag="VYN16_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1402920.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPVPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31301..34093
                     /locus_tag="VYN16_000332"
     CDS             31301..34093
                     /locus_tag="VYN16_000332"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1402921.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10549 a         6072 c         7452 g        10229 t
ORIGIN      
        1 attagtgcta gaataataga ttagaattat tttagaaaaa cgaagtgagc agcttataaa
       61 ttcaagtccc caaatagatt catactagta tcttttgcaa aaaataaagg gcgacttcct
      121 tcatgaatat caatttcatc tataaggaag gtagctaatt gaactaactt atttattctg
      181 tttgtcgcta gaaaaatcag acctccttgt gaagattgag gagatactga atgaaaatca
      241 aagaagaaac tagcaagcta gtagcagatt gcccaaaaca ccgctttgag gttgtagata
      301 agactgacgt atataatcca aggtgaagcg actgtggttt gaagagattt tcaaagagta
      361 taggctagag agtagtgttt ttatgtcctt ctagtagaaa atgctagaca gaagaatggg
      421 gaacttggat aggagaaata gattgagaaa ggaggttaga agagatgatt attacaaaaa
      481 ttagccgttt aggaacttat gtgggagtaa atccacattt tgcaacatta atagattttc
      541 tagaaaaaac aggactagaa aatttaacag aaggttcgat tgctatcgat ggtaatcgat
      601 tgtttgggaa ttgctttact tatctagcag atggtcaagc aggggctttc tttgaaacac
      661 accaaaaata tttggatatt catttagttt tggaaaacga agaagccatg gctgttacat
      721 cgccggaaaa tgtaagcgtt acccaagaat atgatgaaga gaaagatatt gaattataca
      781 cagggaaagt ggaacagttg gttcatttga gagctggtga atgcctcatc acttttccag
      841 aagatttaca tcaacccaag gttcgtataa atgatgaacc tgtgaaaaaa gttgtcttta
      901 aagttgcgat ttcttaatgt agaaagagaa gaacgatgaa aaaaatgaga aagtttttat
      961 gtctagctgg aattgcgcta gcggctgttg ccttggtagc ttgttcagga aaaaaagaag
     1021 ctacaactag tactgaacca ccaacagaat tatctggtga gattacaatg tggcactcct
     1081 ttactcaagg accccgttta gaaagtattc aaaaatcagc agatgctttc atgcaaaagc
     1141 atccaaaaac gaaaatcaag attgaaacat tttcttggaa tgacttctat actaaatgga
     1201 ctacaggttt agcaaatgga aatgtgccag atatcagtac agctcttcct aaccaagtaa
     1261 tggaaatggt caactcagat gctttggttc cgctaaatga ttctatcaag cgtattggac
     1321 aagataaatt taacgaaact gccttaaatg aagcaaaaat cggagatgat tactactctg
     1381 ttcctcttta ttcacatgca caagtcatgt gggttagaac agatttgtta aaagaacata
     1441 atattgaggt tcctaaaact tgggatcaac tctatgaagc ttctaaaaaa ttgaaagaag
     1501 ctggagttta tggcttgtct gttccgtttg gaacaaatga cttaatggca acacgtttct
     1561 tgaacttcta cgtacgtagt ggtggaggaa gcctcttaac aaaagatctt aaagcagact
     1621 tgacaagcca acttgctcaa gatggtatta aatactgggt taaattgtat aaagaaatct
     1681 cacctcaaga ttctttgaac tttaatgtcc ttcaacaagc taccttgttc tatcaaggaa
     1741 aaacagcatt tgactttaac tctggcttcc atatcggagg aattaatgcc aacagtcctc
     1801 aattgattga ttcgattgat gcttatccta ttccaaaaat caaagagtct gataaagacc
     1861 aaggaattga aacctcaaac attccaatgg ttgtttggaa aaattcaaaa catccagaag
     1921 ttgctaaagc attcttagaa gcactttata atgaagaaga ctacgttaaa ttccttgatt
     1981 caactccagt aggtatgttg ccaactatta gggggattag cgattctgca gcctataaag
     2041 aaaatgaaac tcgtaagaaa tttaaacatg ctgaagaagt aattactgaa gctgttaaaa
     2101 aaggtactgc tattggttat gaaaatggac caagtgtaca agctggtatg ttgactaacc
     2161 aacacattat tgaacaaatg ttccaagata tcattacaaa tggaacagat cctatgaaag
     2221 cagcaaaaga agcagaaaaa caattaaatg atttatttga ggctgttcag tagatgtaaa
     2281 agactagaaa ataggtgggc tagtgagctg aaaagttcta gcccaatctt gtaaaagaag
     2341 ggagaaggag aatggttaaa gaacgtaatt taactcgctg gatatttgtt ttgccagcta
     2401 tgattatcgt aggattactc tttgtttatc cgtttttctc gagtattttt tatagcttta
     2461 ccaataagca tttgattatg cctaattata aatttgttgg tttggctaac tataaagctg
     2521 tgctatcaga tcccaacttc tttaatgcgt tctttaattc aattaagtgg accgttttct
     2581 cattagttgg tcaagtttta gtagggtttg tattggcttt agctcttcac agagtacgcc
     2641 acttcaagaa attatatagg accttattga ttgttccttg ggcatttcct accatcgtta
     2701 ttgccttctc ttggcagtgg attctaaacg gggtttatgg ctacttacct aatctaatcg
     2761 taaaattagg tttaatggaa catacacctg catttttgac agatagtaca tgggcattcc
     2821 tatgtttggt gtttatcaac atttggtttg gagcaccaat gattatggtt aatgtgcttt
     2881 cagctttgca aacagtacca gaagaacaat ttgaggctgc taagatagat ggtgcttcaa
     2941 gttggcaggt gttcaagttt atcgtctttc cacatattaa agtggttgta ggacttctag
     3001 ttgttttgag aactgtatgg atctttaata actttgacat tatctacctc attactggtg
     3061 gtggaccagc caatgctaca acgacgcttc caatttttgc ttacaacctg ggttggggaa
     3121 ctaaattgtt gggtcgtgct tcagcagtta cagtattgct ctttatcttc ttggtggcga
     3181 tttgctttat ctactttgct atcatcagta agtgggaaaa ggagggtaga aaataatgaa
     3241 gaagaaatcc agtatttatt tagatattct ctcacatgta cttttagttg gtgcgaccat
     3301 cgttgcaatt ttcccattgg tatggattat catatcttct gtcaaaggga aaggggaatt
     3361 aactcagtat ccaacacgat tttggcctga acagtttaca ttagattatt tcactcatgt
     3421 tatcaacgat ttgcacttca ttgataacat tcgaaacagt ttaatcattg ccttggctac
     3481 aaccctcatt gcgattatta tttctgctat ggcagcctat ggtattgttc gattctttcc
     3541 taaattggga gcaatcatgt cgagactact cgtcattacc tacattttcc caccaatttt
     3601 gttagcaatt ccctattcaa ttgccattgc taaagttggg ttaacaaata gtttatttgg
     3661 cttgatgatg gtttatctat cttttagtgt tccatatgca gtttggctct tagttggatt
     3721 tttccaaaca gttccaattg gaattgaaga agcggctaga attgatggtg caaataaatt
     3781 tgttacgttt tataaggttg tgctaccgat tgtagcacca ggtattgtag caacagctat
     3841 ttatacattt atcaatgctt ggaatgaatt cctgtatgcc ttgattttaa ttaacaatac
     3901 aggaaagatg acagtagcag tagcccttcg ttcacttaat ggttcagaaa tactagactg
     3961 gggagatatg atggcagcgt ctgttattgt agttcttcca tcaattattt tcttctctat
     4021 catccaaaat aagattgcaa gtggattatc agaaggatct gtgaagtaga cgaaagaagg
     4081 aaaaaaatga ataaaagagg tctttattca aaactaggaa tttctgttgt aggcattagt
     4141 cttttaatgg gagtccccac tttgattcat gcgaatgaat taaactatgg tcaactgtcc
     4201 atatctccta tttttcaagg aggttcatat caactgaaca ataagagtat agatatcagc
     4261 tctttgttat tagataaatt gtctggagag agtcagacag tagtaatgaa atttaaagca
     4321 gataaaccaa actctcttca agctttgttt ggcctatcta atagtaaagc aggctttaaa
     4381 aataattact tttcaatttt catgagagat tctggtgaga taggtgtaga aataagagac
     4441 gcccaaaagg gaataaatta tttattttct agaccagctt cattatgggg aaagcataaa
     4501 ggacaggcag ttgaaaatac actagtattt gtatctgatt ctaaagataa aacatacaca
     4561 atgtatgtta atggaataga agtgttctct gaaacagttg atacattttt gccaatttca
     4621 aatataaatg gtatagataa ggcaacacta ggagctgtta atcgtgaagg taaggaacat
     4681 tacctcgcaa aaggaagtat tgatgaaatc agtctattta acaaagcaat tagtgatcag
     4741 gaagtttcaa atattccctt gtcaaatcca tttcagttaa ttttccaatc aggagattct
     4801 actcaagcta actattttag aataccgaca ctatatacat taagtagtgg aagagttcta
     4861 tcaagtattg atgcacgtta tggtgggact catgattcta aaagtaagat taatattgcc
     4921 acttcttata gtgatgataa tgggaaaacg tggagtgagc caatttttgc tatgaagttt
     4981 aatgactatg aggagcagtt agtttactgg ccacgagata ataaattaaa gaatagtcaa
     5041 attagtggaa gtgcttcatt catagattca tccattgttg aagataaaaa atctgggaaa
     5101 acgatattac tagctgatgt tatgcctgcg ggtattggaa ataataatgc aaataaagcc
     5161 gactcaggtt ttaaagaaat aaatggtcat tattatttaa aactaaagaa gaatggagat
     5221 aacgatttcc gttatacagt tagagaaaat ggtgtcgttt atgatgaaac aactaataaa
     5281 cctacaaatt atactataaa tgataagtat gaagttttgg agggaggaaa gtctttaaca
     5341 gtcgaacaat attcggttga ttttgatagt ggctctttaa gagaaaaaca taatggaaaa
     5401 caggtcccta tgaatgtttt ctacaaagat tcgttattta aagtgactcc tactaattat
     5461 atagcaatga caactagtca gaatagagga gagagttggg aacaatttaa gttgttgcct
     5521 ccgttcttag gagaaaaaca taatggaact tacttatgtc ccggacaagg tttagcatta
     5581 aaatcaagta acagattgat ttttgcaaca tatactagtg gagaactaac ctatctcatt
     5641 tctgatgata gtggtcaaac atggaagaaa tcctcagctt caattccgtt tgaaaatgca
     5701 acagcagaag cacaaatggt tgaactgaga gatggtgtga ttagaacatt ctttagaacc
     5761 actacaggta agatagctta tatgactagt agagattctg gagaaacatg gtcggaagtt
     5821 tcgtatattg atggaatcca acaaacttca tatggcacac aagtatctgc aattaaatac
     5881 tctcaattaa ttgatggaaa agaagcagtc attttgagta caccaaattc tagaagtggc
     5941 cgtaagggag gtcaattagt tgtcggttta gtcaataaag aagatgatag tattgattgg
     6001 aaataccact atgatattga tttgccttcg tatggttatg cctattctgc gattacagaa
     6061 ttgccaaatc atcacatagg tgtactgttt gaaaaatatg attcgtggtc gagaaatgaa
     6121 ttgcatttaa gcaatgtagt tcagtatata gatttggaaa ttaatgattt aacaaaataa
     6181 aggagaaaaa acatggttaa atacggtgtt gttggagcag ggtattttgg agctgaattg
     6241 gctcgctaca tgcaaaagaa tgatggagca gagattactc ttctctatga tccagataat
     6301 gcagaggcga ttgcagaaga attgggagca aaagtagcaa gttccttaga tgagttggtt
     6361 tctagcgatg aagtagattg tgttatcgtc gcaactccaa ataatcttca taaggaaccg
     6421 gttattaagg ctgcacagca tggtaaaaat gttttctgtg aaaaaccaat tgcgctttct
     6481 tatcaagatt gtcgcgagat ggtagatgcg tgtaaagaaa acaatgtaac ctttatggca
     6541 ggacatatta tgaatttctt taatggtgtt catcatgcaa aagaactcat taatcaagga
     6601 gttatcggag acgttctata ttgtcataca gctcgtaatg gttgggaaga acaacaaccg
     6661 tcagtatcat ggaaaaaaat tcgtgaaaaa tcaggtggtc acttgtatca ccacatccat
     6721 gaattggatt gcgttcaatt ccttatgggg ggcatgcctg aaactgtaac catgacaggt
     6781 ggaaatgtgg cccatgaagg tgaacatttc ggtgatgaag atgatatgat ttttgtcaat
     6841 atggaatttt ctaataagcg ttttgctttg ttagaatggg gttcagctta tcgttggggt
     6901 gaacattatg tcttaatcca aggaagcaaa ggtgccatcc gcttagactt attcaactgt
     6961 aaaggaactc ttaagctaga tgggcaagaa agctatttct tgattcacga atcgcaagaa
     7021 gaagatgatg atcggactcg tatctatcat agtacagaga tggatggagc aattgcttat
     7081 ggtaaaccag gtaaacgtac tccattatgg ctatcatctg tcattgataa agaaatgcgc
     7141 tatctgcatg agattatgga aggagctcca gtatcagaag aatttgcaaa acttttgaca
     7201 ggtgaagctg ccctagaagc aattgctact gcagatgctt gtacccagtc tatgtttgaa
     7261 gatcgcaaag taaaattgtc agaaattgta aaataaattt tggtattctc ctatttatag
     7321 gtcgacttgc tcctctgaaa gtacttttag aggagctgtt tgactttgct agtttttgaa
     7381 actgaaatct attatactac aaactattga aagcgtttta attttaaggt ataataatct
     7441 catagaaata aagaaaagga ggaaagagga tgccacagat tagcaaagaa gccttgattg
     7501 agcaaatcaa agatggaatc atcgtttctt gtcaggctct tcctcatgaa ccgctttata
     7561 cagaagcggg aggggtgatt cccttgctgg tcaaagcggc tgagcaaggt ggagcagtcg
     7621 gtatccgagc aaacagtgtt cgcgatatca aggaaattaa ggaagtcact aaacttccaa
     7681 tcattgggat tatcaaacgt gattatccac ctcaggaacc cttcatcacg gctactatga
     7741 aagaagttga tgaattggca gaactggaca tcgaggtgat tgctctggat tgtaccaagc
     7801 gtgaacgcta cgatggtttg gaaattcaag agttcattcg tcaggttaag gagaaatatc
     7861 ctaatcagct tttgatggct gatactagta tcttcgaaga agggctagca gctgtagaag
     7921 caggaattga ctttgtcgga acaaccttat caggctacac atcctacagt ccaaaagtag
     7981 acggtccaga ttttgaattg attaagaaac tctgtgatgc cggtgtagat gtcattgcag
     8041 aaggaaaaat tcatacacca gaacaagcca aacaaatcct tgaatatgga gtgcgaggca
     8101 tcgttgttgg tggcgccatt actagaccaa aagagattac agaacgcttc gttgctagtc
     8161 ttaaataaga tgtgaggggg agttttatgt ttaaagtttt acaaaaagtt ggaaaagctt
     8221 ttatgttacc tatagctata cttcctgcag caggtctact tttggggatt ggtggtgcac
     8281 tttcaaaccc aaccacgata gcaacttatc caatactaga caatagtatt tttcaatcaa
     8341 tattccaagt aatgagctct gcaggagagg ttgtattcag taatttgtca ctacttctct
     8401 gtgtgggatt atgtattggc ttagcgaaac gagataaagg aaccgctgcg ttagcaggag
     8461 taactggtta cttagttatg actgcaacga tcaaagcttt ggtaaaactt tttatggcag
     8521 aaggatctgc aattgatact ggagttattg gagcattagt tgtcggaata gttgccgtat
     8581 atttgcacaa ccgatataac aatattcaat taccttccgc tttaggattc tttggaggtt
     8641 cacgcttcgt tcctattgtt acatcgttct cttctatctt gattggcttt gtcttctttg
     8701 ttatttggcc acctttccaa caacttcttg tttctacagg tggatatatt tctcaggcgg
     8761 gtccaattgg aacttttcta tatggatttt taatgagact ttctggagca gtaggcttac
     8821 atcatataat ttaccctatg ttttggtata ctgaacttgg tggtgttgaa actgttgcag
     8881 gacaaacagt ggttggagct caaaaaatat tttttgctca attagccgat ccggcccatt
     8941 ctggattatt tacagaagga acaaggtttt ttgcaggtcg tttctcaaca atgatgttcg
     9001 gtttaccggc tgcctgttta gcgatgtacc atagtgttcc taaaaatcgt cgtaaaaaat
     9061 acgcgggttt gttttttgga gttgctttaa catcttttat taccggtatt acagaaccaa
     9121 ttgaatttat gtttctattc gtcagtccgg ttctatatgt tgttcacgca ttccttgatg
     9181 gtgttagctt ctttattgca gacgtcttaa atatttcaat aggaaacaca ttttcaggag
     9241 gtgtaatcga tttcacttta tttggaattt tgcaggggaa cgctaagacg aattgggttc
     9301 ttcagattcc aattggactt atttggagtg ttttgtatta tattattttt agatggttca
     9361 ttactcaatt caacgttcta acgccaggac gaggagaaga agtagattct aaagaaattt
     9421 ctgaatccgc agattcaact tcaaatactg cagattattt aaaacaggat agcctacaaa
     9481 ttatcagagc cttgggtgga tcaaataata tagaagatgt agatgcttgt gtgacacgtt
     9541 tacgtgtagc tgtaaaagaa gttaatcaag ttgataaagc acttttaaaa caaattggtg
     9601 cagttgatgt cttagaagtg aagggtggca ttcaagcaat ctatggagca aaagcaatct
     9661 tatataaaaa tagtattaat gaaattttag gtgtagatga ttaagtactt actgacttaa
     9721 taaaaaacag aggagagtga tggatgagta ggatgaaatg aaatcgcata caagaaataa
     9781 agaactcatt atccaagttg gatacgctta ttacatagga gaatacaaat gaaatttaga
     9841 aaattagctt gtacagtact tgcgggtgct gcggttcttg gtcttgctgc ttgtggcaat
     9901 tctggcggaa gtaaagatgc tgccaaatca ggtggtgacg gtgccaaaac agaaatcact
     9961 tggtgggcat tcccagtatt tacccaagaa aaaactggtg acggtgttgg aacttatgaa
    10021 aaatcaatca tcgaagcgtt tgaaaaagca aacccagata taaaagtgaa attggaaacc
    10081 atcgacttca agtcaggtcc tgaaaaaatc acaacagcca tcgaagcagg aacagctcca
    10141 gacgtactct ttgatgcacc aggacgtatc atccaatacg gtaaaaacgg taaattggct
    10201 gagttgaatg acctcttcac agatgaattt gttaaagatg tcaacaatga aaacatcgta
    10261 caagcaagta aagctggaga caaggcttat atgtatccga ttagttctgc cccattctac
    10321 atggcaatga acaagaaaat gttagaagat gctggagtag caaaccttgt aaaagaaggt
    10381 tggacaactg atgattttga aaaagtattg aaagcactta aagacaaggg ttacacacca
    10441 ggttcattgt tcagttctgg tcaaggggga gaccaaggaa cacgtgcctt tatctctaac
    10501 ctttatagcg gttctgtaac agatgaaaaa gttagcaaat atacaactga tgatcctaaa
    10561 ttcgtcaaag gtcttgaaaa agcaactagc tggattaaag acaatttgat caataatggt
    10621 tcacaatttg acggtggggc agatatccaa aactttgcca atggtcaaac atcttacaca
    10681 atcctttggg caccagctca aaatggtatc caagctaaac ttttagaagc aagtaaggta
    10741 gaagtggtag aagtaccatt cccatcagac gaaggtaagc cagctcttga ataccttgta
    10801 aacgggtttg cagtattcaa caataaagac gacaagaaag tcgctgcatc taagaaattc
    10861 atccagttta tcgcagatga caaggagtgg ggacctaaag acgtagttcg tacaggtgct
    10921 ttcccagtcc gtacttcatt tggaaaactt tatgaagaca aacgcatgga aacaataagc
    10981 ggctggactc aatactactc accatactac aacactattg atggatttgc tgaaatgaga
    11041 acactttggt tcccaatgtt gcaatctgta tcaaatggtg acgaaaaacc agcagatgct
    11101 ttgaaagcct tcactgaaaa agcgaacgaa acaatcaaaa aagctatgaa acaatagtcc
    11161 ttagttattc tataaaaagt agttttttaa agaacctaag agtgtatacc cccttttccc
    11221 tctacacaga tagtgtaaga aaagggggct tttgtttaaa atgtaagaaa ctgtcacgaa
    11281 attaaaatga agttcttaca taagcgaatc ataaaaaatt tcattttgat tttaaaacag
    11341 ttcaagaaag tcaaaaaatt attctatttg aaagagaggt gccgactgtg aaagtcaata
    11401 aaatccgtat gcgggaaaca gtgatttcct acgctttcct agcaccagta ttattcttct
    11461 ttgtcatctt tgtgttggct ccgatggtga tgggcttcat tacaagtttc tttaactact
    11521 caatgactaa atttgagttt gtaggcttgg ataactatat ccgtatgttt aaagatcctg
    11581 tctttacaaa atctctgatt aacacagtta ttttggttat tggatctgta ccagttgttg
    11641 ttctattctc actctttgta gcatctcaga cctatcatca aaatgtcatt gccagatcct
    11701 tctaccgttt cgtcttcttc cttcctgttg taacgggtag tgttgccgtg acagttgttt
    11761 ggaaatggat ttatgaccca ctatcaggga ttctaaactt tgtccttaag tccagccaca
    11821 tcatcagcca aaacatttct tggttgggag ataaaaactg ggcattgatg gcgattatga
    11881 ttattctctt gaccacttca gttggtcagc ccatcatcct ttatatcgct gccatgggga
    11941 atattgacaa ttcactggtt gaagcggcgc gtgttgatgg tgcaactgag tttcaagttt
    12001 tttggaagat taaatggcca agccttcttc caacaactct ttatattgca atcatcacaa
    12061 caattaactc attccagtgt tttgccttga ttcagctttt gacatctggt ggtccaaact
    12121 actcaacaag taccttgatg tactaccttt acgaaaaagc cttccaattg acagaatacg
    12181 gctatgccaa cacaattggt gtcttcttgg cagtcatgat tgctatcgta agctttgttc
    12241 aatttaaagt acttggaaac gacgtagaat actaaagaaa ggagacagct atgcaatcta
    12301 cagaaaaaaa accattaaca gcctttactg ttatttcaac aatcattttg ctcttgttga
    12361 ctgtgctgtt catctttcca ttctactgga ttttgacagg ggcattcaaa tcacaacctg
    12421 atacaattgt tattcctcct cagtggttcc ctaaaatgcc aaccatggaa aacttccaac
    12481 aactcatggt gcagaaccct gccttgcaat ggatgtggaa ctcagtattt atctcattgg
    12541 taaccatgtt cttagtttgt gcaacctcat ctctagcagg ttatgtattg gctaaaaaac
    12601 gtttctatgg tcaacgcatt ctatttgcta tctttatcgc tgctatggcg cttccaaaac
    12661 aagttgtcct tgtaccattg gtacgtatcg tcaacttcat gggaatccac gatactctct
    12721 gggcagttat cttgcctttg attggatggc cattcggtgt cttcctcatg aaacaattta
    12781 gtgaaaatat tcctacagag ttgcttgaat cagctaaaat cgacggttgt ggtgagattc
    12841 gtaccttctg gagtgtagcc ttcccgattg tgaaaccagg gtttgcagcc cttgcaatct
    12901 ttaccttcat caatacttgg aatgactact tcatgcaatt ggtaatgttg acttcacgta
    12961 acaatttgac catctcactt ggggttgcga ccatgcaggc tgaaatggca accaactatg
    13021 gtttgattat ggcaggagct gcccttgctg ctgttccaat cgtcacagtc ttcctagtct
    13081 tccaaaaatc cttcacacag ggtattacta tgggagcggt caaaggataa tactctgaga
    13141 aaatctcttc aaactacgtc agcgtcacct tgccatactt aagtattgcc tgcggttagc
    13201 ttcctagttt gttcttcaat tttcattgag tataggaaaa tcaatctatc aagatacaga
    13261 agtatatttt atagatttag agaatataga ggttataagt gtctacaaaa tggagggtat
    13321 gcagttactt tatgaagttt tgtcagacac ttataaactt aagaatggtt ttagttaact
    13381 atcagaaacg aaggaaagag tatgattttt gacgatttga aaaacatcac cttttacaaa
    13441 gggattcatc ctaatttaga caaggctatc gactatctct accaacatcg taaggattct
    13501 ttcgaattag gaaagtatga tattgatgga gataaagtct ttctagttgt tcaggaaaat
    13561 gtcctcaatc aagctgaaaa tgatcaattt gagtatcata agaactatgc agatttgcat
    13621 ttgctggtag aaggacatga atattcgagc tacggttcac gtatcaaaga cgaggcagta
    13681 gcattcgacg aagcgagtga cattggcttt gttcattgtc atgaacacta cccactcttg
    13741 ttgggttatc acaattttgc gattttcttc ccaggtgagc cacatcagcc aaatggttat
    13801 gcaggcatgg aagaaaaggt tcggaaatat ctctttaaaa ttttgattga ttaaaaatag
    13861 gatgaattgt ttttttgtaa agctttgaca atactctacc atgaaattga tctttgtgag
    13921 gtagggaaat gagaataaaa tatttaaaaa ttggtatctt ctaagtatgc tgcaagagct
    13981 agtttcttgg atggacaggg aattacagtt gatgagatgg cttggataat taggggcatt
    14041 gtgaatggat tgattggtag atacataaaa ttaggtactt atgcagccaa gtatggtatt
    14101 agtatggcgc gttcgatctt aagtagggca gctgcaactg cagcagcaag agtaggatta
    14161 tcgaccaaga tttctggatg gattttacga gtagctgtga atgtagctga tgtatatggt
    14221 aattttgcca acaatattgc tgcagcttgg gatgcacatg ataaaattcc taacaatggt
    14281 cgtataaact tttaaaatgc gagaatgaaa gcactttgta tttttttatt gaatatgtta
    14341 gcttggacag tgcttgcaat gataattcgt ggagggctag atggatttga tgagcatact
    14401 tggagtacta ttttaattgc ttcgctgttc ggggtatatg attataagcc catagataaa
    14461 aatagaaaaa agtccaaaag aaaaaagaaa aaagaaaaaa tagatttgtt catggtaggg
    14521 acttatgaaa gctttactga caaaaaagaa aacagtttac aaagaaaaat gatggaggag
    14581 caaacatggc acaaaaagga gtaagcctta tcaaggcagc atttgataca gacaactttc
    14641 tcatgcgttt tagtgagaaa gtcttggaca tcgtgacagc caatcttctt tttgtcgtct
    14701 cttgtttgcc catcgtgacg attggagtgg ctaaaatcag cctctacgag accatgttcg
    14761 aagttaagaa gagcagacgg gtgcctgttt ttaaaatcta tctaagatct ttcaagcaaa
    14821 atctgaaact aggtcttcag ctgggtttaa tggagttagg aattgtgttt cttacccttt
    14881 cagatctcta tcttttctgg ggtcaaacag ctctgccctt ccaattgctg aaagccattt
    14941 gtttaggtat tctgattttt cttactatcg tgatgctggc tagttaccct atcgcggcac
    15001 gttatgacct atcttggaaa gaaattcttc aaaaaggatt gatgttggct agttttaact
    15061 ttccttggtt cttcctcatg ttagccattc ttgtcctcat tgtgatggtt ctttatctgt
    15121 ccgccttcag tctactctta ggtggctcag tcttcttact ttttgggttt ggactattgg
    15181 tctttatcca gactggattg atggagaaaa ttttcgcaaa ataccaatag gagccttatt
    15241 tctgaaacta ctttcaaacg ctccaaacgc tattctataa gcgagaaact aaaatctaag
    15301 tgtctataag atattgaaag ccttttcggt aaagtgtata ctaaagaagt aaagaaaaat
    15361 ctgctttagc agaaaataaa aataatagga gaaaatctat gtcagatttg aaaaaatacg
    15421 aaggtgtcat tccagccttc tacgcatgtt atgatgatca aggagaagta agcccagaac
    15481 gtacgcgtgc cttggttcaa tacttcattg ataaaggtgt tcaaggtctt tatgtcaatg
    15541 gttcttctgg tgaatgtatc taccaaagtg ttgaagaccg caagttaatc ttggaagaag
    15601 tcatggcggt agccaaaggt aaattgacca tcattgctca tgttgcctgc aacaatacta
    15661 aagatagtat ggaacttgct cgccatgctg aaagcttggg agtagatgct attgcaacga
    15721 ttccaccaat ttatttccgc ttgccagaat actcagttgc caaatactgg aacgatatca
    15781 gttctgcagc tccaaacaca gactacgtga tttacaacat tcctcaattg gcaggggttg
    15841 ctttgactcc aagcctttac acagaaatgt tgaaaaatcc tcgtgttatc ggtgttaaga
    15901 actcttctat gccagttcaa gatatccaaa cctttgtcag ccttggtggg gaagaccata
    15961 tcgtctttaa tggtcctgat gagcagttcc taggaggacg cctcatgggt gctagggctg
    16021 gtatcggtgg tacttatggt gctatgccag aactcttctt gaaactcaat cagttgattg
    16081 cggataagga cctagaaaca gcgcgtgaat tgcagtatgc tatcaacgca atcattggta
    16141 aactcacttc tgctcatgga aatatgtacg gtgtcattaa agaagtcttg aaaatcaatg
    16201 aaggcttgaa tattggatct gttcgttcac cattgacacc agtgactgaa gaagatcgtc
    16261 cagttgtaga agcggctgct gccttgattc gtgaaaccaa ggagcgcttc ctctaatata
    16321 aaaggaggta tttatgacat actacgttgc aattgatatc ggtggaacca acatcaagta
    16381 tggtttggtt gatcaagagg ggcaacttct tgaatcgcat gaaatgccaa ctgaggcgca
    16441 taagggtgga cctcatatct tacaaaagac caaagatatc gtagctagtt atttagaaaa
    16501 aggcccagta gcaggtgttg ccatatcttc tgctgggatg gtggatccgg ataagggtga
    16561 gattttctat gctgggcctc aaatccctaa ctacgcaggc acccagttca aaaaggaaat
    16621 cgaagaaagc tttactattc cttgtgagat tgaaaatgat gtcaactgtg caggtcttgc
    16681 tgaggcagta tctggttcag gcaagggagc aagtgtgaca ctttgcttga ccattggaac
    16741 cggtatcggt ggttgcttga ttatggatag gaaagtcttc catggtttta gcaattcagc
    16801 ctgtgaagtc gggtatatgc atatgcagga tggagctttt caggatctcg cttctacaac
    16861 agctttagtg aaatatgtag ctgaagccca tggagaagat gttgatcagt ggaatggccg
    16921 acgtatcttt aaggaagcta ctgaaggaaa caaaatctgc atggaaggta ttgaccgtat
    16981 ggttgactat ctaggaaaag gtctggcaaa tatttgttat gtggccaatc cagaagtggt
    17041 tattcttggt ggtggtatca tggggcaaga ggctatcctc aaacctaaga tccgcacagc
    17101 cttgaaagag gcattggtac caagtttagc agaaaaaaca cgattagaat ttgcccatca
    17161 ccaaaataca gcagggatgt tgggtgcata ttatcatttt aagacaaaac aatcctagtt
    17221 tggctcagcc aaactaggat tttcttacac gtttttgtct acgatagccg ttgagttttt
    17281 tattttccca gtagctatta aagatttttt ccttgctttc gcgattgatt tccaaaaagt
    17341 aggcataaat caaatcgata aagaagagca taggaagttg agcggatatt cgttggatgt
    17401 aggagggttg gctgtgggtg gctacaagaa cagtctctgt ataggtctgg ctatctttat
    17461 tgggaacact tgtaaagagt acagtctttg cccccatctc cttggcatct aatagactat
    17521 ctaaaataga aggagttgag cctgaaagtg agaaacccag tactagacaa ttttcatcca
    17581 tgatgctggt tgtccaggca aagccgtctt ggtctgtcaa agcttcgcag accacaccta
    17641 gtcgcataaa acgtaatttc atttcacggg cgacgaggcc agaactccct gttccaaaga
    17701 agtagatacg ctcagcatct tcgattagct gggcaattcg ctctagttgg atttcgtcaa
    17761 tcaagtcttg tgtttgttcc ctcatgttgc tataacttct gaggactcgt ttggtcagtg
    17821 gactgtgctt ggaaacttgg ttggcttgat tttctgcctg atgttggtat tggaaaataa
    17881 attctcggta gccagtaaag ccacactttt tagcaaagcg ggtcaaagca gcttgagaaa
    17941 tatgtaattt ttgggtgact tgttgagaag ataaatcatc tgtaatcgtt tcagcttgca
    18001 aaaaatagcg agcgatttct tgctctaggt ctgtcatttc ttcaaaatgt gaatcaatga
    18061 tagttgcgat atctggtttg tccataggga aagctccttt acatgagtca tactggaaga
    18121 ctagatcagg gaatagtcac acttcattat aacacataat ataaggatag ataaataaaa
    18181 acgcatctct gttttaaaaa cgaaaaaatc gaaaaagctt ctctcttttc cataattttc
    18241 tactcaaatt gtggtacaat taagagtaag attttaagtt agaaatgaga ctgatttgta
    18301 tgagaaaatt taacagccat tcgattccga ttcggcttaa tttattgttt tcaatcgtca
    18361 ttttactctt tatggccatt attggtcgtt tgttgtatat gcaggttttg aacaaggatt
    18421 tttacgaaaa aaagctagcc tcagctagtc agaccaagat tacaagcagt tcagcccgtg
    18481 gggaaattta tgatgctagt ggaaaacctt tggtagaaaa tacgttaaag caggttgttt
    18541 cctttacgcg tagcaataaa atgacggcta cagacttaaa agaaacagct aaaaagttac
    18601 tgacttatgt gagcatcagt tctccaaatt tgacagaacg ccagctggcg gattactatt
    18661 tggctgatcc tgaaatctat aaaaaaacag tggaagctct cccaagcgag aaacgcttgg
    18721 attcagatgg caatcgtcta tccgaatcag aactgtataa caatgcggtc gatagtgtac
    18781 aaacgagtca actaaactat acagaggatg aaaagaaaga aatctatctt tttagtcagt
    18841 taaatgctgt tggaaacttt gcgacaggaa ccattgcgac agatcctcta aatgattctc
    18901 aggtggctgt tattgcctct atttcaaagg agatgcctgg cattagtatt tctacttctt
    18961 gggatagaaa ggttttggaa acttcccttt cttctatagt tgggagtgta tccagtgaaa
    19021 aagctggtct cccagcggaa gaagcagaag cctatcttaa aaaaggctat tctctaaatg
    19081 atcgtgtagg aacctcctat ttggaaaagc aatatgaaga gaccttacaa ggaaaacgct
    19141 cggtaaaaga aatccatctg gataaatatg gcaatatgga aagcgtggat acaattgagg
    19201 aaggtagtaa gggaaacaat atcaaactga ccattgattt ggctttccaa gatagcgtgg
    19261 atgctttact gaaaagttat ttcaattccg agctagaaaa tggtggagcc aagtattctg
    19321 aaggtgtcta tgcagtcgcc cttaacccaa aaacaggtgc ggttttgtct atgtcaggga
    19381 ttaaacatga cttgaaaacg ggagagttga cgcctgattc cttgggaacg gtaaccaatg
    19441 tctttgtgcc gggttctgtt gtcaaggcgg cgaccatcag ctctggttgg gagaatggag
    19501 tcttatcagg gaatcagacc ttgacagacc aaccgattgt cttccaaggt tcagctccga
    19561 ttaattcttg gtacactcag gcttacggtt cattcccgat tacagctgtg gaagccttgg
    19621 agtattcttc taatgcctat atggttcaaa cggctttggg cattatgggt cagacctatc
    19681 aacccaatat gtttgttcta actaacaatt tagaatccgc catggggaaa cttcgctcga
    19741 catttgcgga atatggtctt ggagcctcaa caggcattga ccttccagat gagtcaactg
    19801 gttttatacc aaaagagtat aattttgcta attacattac caatgccttt ggtcagtttg
    19861 ataactacac tcctatgcaa ttggcccagt acgttggaac cattgccaac aacggtgttc
    19921 ggattgcacc tcacattgtc gaggggattt atggaaacaa tgaacaaggc ggcttaggga
    19981 acttaatcca atctgttgaa tccaaggaaa tgaataaaat taatatttct gagtctgatg
    20041 tttccatcct ccaacaaggc ttttatcaag tgtcacatgg tggtagtgct ttgacaaccg
    20101 gtcgtgcctt ttcaaatggc gcaagcgtat cgattagtgg taaaacaggt actgccgaaa
    20161 gttatgttga gggtggtcaa gaagctaaca atactaatgc tgtggcctat gcaccatcag
    20221 ataatcctca aatcgctgta gctgttgtct tccctcataa caccaacctt acaaatggtg
    20281 tcggaccttc cattgcgcgc gatattatca acctctataa ccaacatcat ccaatgaatt
    20341 agaaaggaac atatgcttta tccaacacct attgctaagc taattgacag ttattctaag
    20401 ttaccgggta tcgggattaa gacggctaca cgtctggcct tctatactat tggaatgtct
    20461 gatgacgatg tcaatgaatt tgctaaaaat ctcctttctg ctaagagaga gctgacctac
    20521 tgttccatct gcggtcgcct gactgatgac gatccttgct ctatctgtac cgatccgact
    20581 cgtgaccaga caacgatttt agttctcgaa gatagtcgcg atgtggcagc catggagaat
    20641 atccaagaat accatggact ctatcatgtc ttgcatggac tcatttctcc catgaatggt
    20701 attagtccag atgatatcaa tctcaagagc ctcatgactc gtcttatgga tagtgaagtt
    20761 tcagaggtaa tcgtagcaac aaatgctaca gcagatggtg aagcgacatc catgtatctt
    20821 tcacgtttgc tcaagccagc tggtatcaag gttacgcgtc tagcacgagg tctcgctgtc
    20881 ggagcggaca ttgagtatgc ggatgaagtg acgctcttac gagccattga aaatcgaaca
    20941 gagttgtaag tgtaggcaaa tttacgaact ccattcattt ataaaaaatc aaagaggctg
    21001 aaaatcgttc ctatcggcct ctttttgtat agtgtgatga gtaggatcag gttcaagttt
    21061 taaaaaacca agcaaatatg atatactaaa gagcgagtat tctagtagaa ttaggacgaa
    21121 taatatgaaa caaacgatta ttcttttata tggtggacgg agtgcggaac gcgaagtctc
    21181 tgtcctttca gctgagagtg tcatgcgtgc ggtcgattac gaccgtttca cagtcaagac
    21241 tttctttatc agtcagtcag gtgactttat caaaacacag gaatttagtc atgatccggg
    21301 gcaagaagac cgtctcatga ccaatgaaac cattgattgg gataagaaag ttgcaccaag
    21361 tgctatctac gaagaaggtg cagtggtctt tccagtcctt cacgggccaa tgggagaaga
    21421 tggctctgtt caaggattct tggaagtttt gaaaatgcct tacgttggtt gcaacatttt
    21481 gtcatcaagt cttgccatgg ataaaatcac gactaagcgt gttctggaat ctgctggtat
    21541 tgcccaagtt ccttatgtgg ctatcgttga aggcgatgat gtgactgcta aaatcgctga
    21601 agtggaagaa aaattggctt atccagtctt cactaagccg tcaaacatgg ggtctagtgt
    21661 cggtatttct aagtctgaaa accaagaaga actccgtcaa gccttagaac ttgccttccg
    21721 atatgatagc cgtgtcttgg ttgagcaagg agtgaatgcc cgtgaaattg aggttggcct
    21781 cttgggtaac tacgatgtca agagcacgct accaggagaa gttgtcaagg atgttgcctt
    21841 ttatgactac gatgccaagt atattgataa caagattact atggatattc ctgccaaaat
    21901 cagtgatgat gtggtggctg tcatgcgtca aaatgcagaa acagccttcc gtgccattgg
    21961 tggccttggt ctatctcgtt gcgatttctt ctatacagat aagggagaga tttttctcaa
    22021 cgagctcaat accatgccag gtttcaccca gtggtctatg tacccactac tttgggacaa
    22081 tatggggatc agctacccag aactaatcga gcgtttggtt gaccttgcca aggaaagttt
    22141 tgacaagcgc gaagcgcatt tgatataaaa tgaaagagag ggtagaagcc agaaccatca
    22201 ctgcacggtg actagagttc tcggacttca gcccttttta aaggagtaga aatgaaatta
    22261 acaatccatg aaattgccca agttgttgga gccaaaaatg atatcagtat ctttgaggac
    22321 acccagttag aaaaagctga gtttgatagt cgtttgattg gaactggaga tttatttgtg
    22381 ccacttaaag gtgcgcgtga tggccatgac tttattgaaa cagcctttga aaatggtgca
    22441 gcagtaacct tgtctgagaa agaggtctca aatcatcctt acattctagt agatgatgtt
    22501 ttgacagcct ttcaatccct agcatcctac tatcttgaaa aaacgactgt tgatgtcttt
    22561 gctgttacag gttcaaatgg caagacaacg actaaggata tgttggcgca tttactgtca
    22621 acaagataca agacctacaa aacacaaggc aattacaata acgagattgg ccttccttac
    22681 acagttcttc atatgcctga agggacagaa aagttggtct tggagatggg gcaggatcac
    22741 ttgggagata ttcatctctt gtctgaattg gctcgtccaa aaacagccat cgtgaccttg
    22801 gttggagaag cccatttggc ctttttcaaa gaccgttcag agattgctaa gggaaaaatg
    22861 caaattgcag acggaatggc ttcaggttcc ttgcttttag cgccggctga ccctatcgta
    22921 gaggactatt tgccaactga taaaaaggtg gttcgttttg gtcaaggagc agagctggaa
    22981 attactgact tggttgagcg caaagatagt ctgaccttca aggccaattt cttagagcaa
    23041 gtccttgatt tgccagtgac tggcaagtac aatgcgacaa atgctatgat tgcatcctat
    23101 gttgccttgc aagaaggagt ttcagaggag caaattcatc aggccttcca aggtcttgaa
    23161 ttgacgcgta atcgtactga gtggaagaaa gcagccaatg gagcagatat cctgtcagac
    23221 gtatataatg ccaatccaac tgctatgaag ctgattttag agactttctc tgccattcca
    23281 gccaatgaag gtggcaagaa aattgcagtg ttggcggata tgaaggagct tggtgaccag
    23341 tctattcaac tccataacca gatgattttg agcctttctc cagatgtgct tgataccgtt
    23401 attttctatg gagaaaatat tgctgaatta gcccaattgg ctagtcaaat gttcccaata
    23461 ggccacgttt actacttcaa gaaaacagaa gaccaagacc aatttgaaga cctagtcaag
    23521 caggtcaaag aaagccttgg agtccacgac caaatcctgc tcaaaggctc taactctatg
    23581 aatctagcca agttggtaga aagtttagaa aatgaaacca agtgattttg ttaagtatct
    23641 gcaaagaatg attgccctta cagatactgg cttaaccttt acaaaagatc cttttgaccg
    23701 tgagcgctac gaagacttgc gaagtctgtt atctgaaatg ttgaatcagg tatcagacct
    23761 ggatgcagaa gaagtagcag aagtcttgaa accaacttca gcttatgcga ctcccttaat
    23821 ggacgtccgt gcttggattg ttgaggatga gaagatttgt ctggttaggg gacaaggaga
    23881 ggatagttgg gctttgccag gtggctttgg tgaagtcggc tattctccaa ctgaaaatat
    23941 tctcaaggaa attgaagaag aaaccggttt taaagccaaa gttgaaagac tgctagctgt
    24001 ttttgatacc aatcgtttcc aactacagag caaacaatat gcaaagtttg tttttgaatg
    24061 taagcttctc gatggacaat tccaagaaaa tcaagaaatc gctgcccttc aattttttgc
    24121 aattgaccaa ctgccgaatt tatctgaaaa acgcattacc aaggagcaaa tcgagattct
    24181 ttggcaggtt tatcaaggtc agagggagca atatcttgac taagaagatg attatcgtat
    24241 ttctaaatcc atttttgaca actagcatgg tataataata tgcaggaaaa ttttgaatca
    24301 tgaggaagac tagatgaatt tatgggatat tttctttacg actcaggcaa ccgagccgcc
    24361 caaatttgac cttttttggt atgttagcct atttacgctc ttagccttaa ccttttatac
    24421 agcccatcgc tatcgtgaaa agaaggttta ccaacgattt ttccaaatct tgcagactgt
    24481 tcagttaatc cttctttatg gttggtactg ggtcaatcat atgccactgt cagaaagcct
    24541 acccttttac cattgccgta tggctatgtt tgtggtactc ttgcttcctg gtcagtccaa
    24601 atataaacaa tactttgcat tattgggaac atttgggaca ttagcagcct ttgtttatcc
    24661 agtgccagat gcttaccctt ttccacatat caccattcta tcctttatct ttggtcattt
    24721 agcactcttg gggaactctc tagtttatct attgagacag tataatgcgc gattgctgga
    24781 tgtgaaggga atttttctca tgacctttgc cctaaatgcc ttgatttttg tggtcaattt
    24841 ggtgacaggt ggcgattacg gatttttgac aaaaccgcca ttggttgggg atcacggtct
    24901 agtagctaat tatttacttg tttcaattgt gctggtagct actatcagtt tgactaagaa
    24961 aatcttagaa ttctttttag ctcaagaagc agaaaaaatg attgcaaagg aagcttaaca
    25021 cagagctttc ttttttgctc ttagagagtt tttacaagca gcttataaaa taagaatttc
    25081 tgaatagaca aactcaaaaa atggctggga aatttaggaa aaaagcaagc acgattaaat
    25141 tttttgtgtt ataatatttt gtgaatagct atgcctatgt ttagctatgg aataatacga
    25201 agtgcgaaac ttggaagata gagaggaagc gatgtaatgg ctagagaagg cttttttaca
    25261 ggtctagata ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag aaatggtgaa
    25321 ttaaatgtaa ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg aattattgtt
    25381 gatattgatg cagcagcaac tgctatcaag tcagccattt cccaagcgga agaaaaggca
    25441 ggcatttcga ttaaatcagt aaatgtcggc ttgcctggta atcttttgca ggtagaacca
    25501 actcagggga tgattccagt aacatctgat actaaggaaa ttacggatca agatgttgaa
    25561 aatgttgtca aatcagcttt gacaaagagt atgacacctg accgtgaagt cattaccttt
    25621 attcctgaag aatttattgt ggatggtttc caagggattc gtgacccacg tggcatgatg
    25681 ggggttcgcc ttgaaatgcg tggtttgctt tatacaggac ctcgtactat cttgcacaat
    25741 ttgcgtaaga cggttgagcg tgcaggtgtt caggttgaaa atgttatcat ttcaccacta
    25801 gcaatggttc agtctgtttt gaacgaaggg gaacgtgaat ttggtgctac agtgattgat
    25861 atgggggcag gtcaaacgac tgtcgctaca atccgtaatc aagaactcca gttcacacat
    25921 attctccaag aaggtggaga ttatgtaact aaagatatct ccaaggtttt gaaaacctct
    25981 cgcaaattag cggaaggctt gaaactgaat tacggggaag cctatccgcc tcttgcaagc
    26041 aaagaaacct tccaagtaga ggttattgga gaagtagaag cagtcgaagt gacggaagcc
    26101 tacttgtcag aaattatttc tgcacgaatc aagcacatcc ttgaacaaat caagcaagaa
    26161 ttagatagaa ggcgtctatt ggacctccct ggtggtattg tcttaatcgg tgggaatgcc
    26221 attttaccag gtatggttga gcttgctcag gaagtctttg gcgtccgtgt caagctttat
    26281 gttccaaatc aagttggtat ccgtaatcca gcctttgcgc atgtgattag tttatcagaa
    26341 tttgcgggtc aattaacaga agttaatctt ttggctcagg gagcgataaa aggtgagaat
    26401 gacttaagtc atcagccaat tagttttggt gggatgctgc aaaaaacagc tcagtttgta
    26461 caatcaacgc ctgttcaacc agctcctgct ccagaagtag agtcggtggc gcctacagaa
    26521 ccaatggcgg atttccaaca agcttcacaa aataaaccga aattagcaga tcgtttccgt
    26581 ggcttgatcg gaagcatgtt tgacgaataa agaggaaaaa taaattatga cattttcatt
    26641 tgatacagct gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg gaggtggtgg
    26701 caatgccatc aaccgtatgg tcgacgaagg tgttacaggc gtagaattta tcgcagcaaa
    26761 cacagatgta caagcattga gtagtacaaa agctgagact gttattcagt tgggacctaa
    26821 attgactcgt ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag ccgctgaaga
    26881 aagcgaagaa acactgacgg aagctattag tggtgccgat atggtcttca tcactgctgg
    26941 tatgggagga ggctctggaa ctggagctgc tcctgttatt gctcgtatcg ccaaagattt
    27001 aggtgcgctt acagttggtg ttgtaacacg tccctttggt tttgaaggaa gtaagcgtgg
    27061 acaatttgct gtagaaggaa tcaatcaact tcgtgagcat gtagacactc tattgattat
    27121 ctcaaacaac aatttgcttg aaattgttga taagaaaaca ccgcttttgg aggctcttag
    27181 cgaagcggat aacgttcttc gtcaaggtgt tcaagggatt accgatttga ttaccaatcc
    27241 aggattgatt aaccttgact ttgccgatgt gaaaacggta atggcaaaca aagggaatgc
    27301 tcttatgggt attggtatcg gtagtggaga agaacgtgtg gtagaagcgg cacgtaaggc
    27361 aatctattca ccacttcttg aaacaactat tgacggtgct gaggatgtta tcgtcaacgt
    27421 tactggtggt cttgacttaa ccttgattga ggcagaagag gcttcacaaa ttgtgaacca
    27481 ggcagcaggt caaggagtga acatctggct cggtacttca attgatgaaa gtatgcgtga
    27541 tgaaattcgt gtaacagttg ttgcaacggg tgttcgtcaa gaccgcgtag aaaaggttgt
    27601 gtctccacaa gctagaactg ctactaacta ccgtgagaca gtgaaaccag ctcattcaca
    27661 tggctttgat cgtcattttg atatggcaga aacagttgaa ttgccaaaac aaaatccacg
    27721 tcgtttggaa ccaactcagg catctgcttt tggtgattgg gatcttcgcc gtgaatcgat
    27781 tgttcgtaca acagattcag tcgtttctcc agtcgagcgc tttgaagccc caatttcaca
    27841 agatgaagat gaattggata cacctccatt tttcaaaaat cgttaagtaa atgaatgtaa
    27901 aagaaaatac agaacttgtt tttcgagaag ttgcagaggc tagtctgagt gctcatcgag
    27961 agagtggttc ggtctctgtc attgcagtta ccaagtatgt agatgtaccg acagcggaag
    28021 ccttgcttcc gctaggtgtt catcatatcg gtgaaaatcg tgtagataag tttctggaaa
    28081 aatatgaagc tttaaaagat cgagatgtga cttggcattt gattggtacc ttgcaaagac
    28141 gtaaggtgaa agatatcatt caatacgttg attatttcca tgcattggac tcagtaaagc
    28201 tagcagggga aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt caagtaaata
    28261 tttctaaaga agaaagcaaa cacggttttt cgagagagga actgctggaa atcttgccag
    28321 agttagccag actagataag attgaatatg ttggtttaat gacgatggca ccttttgagg
    28381 ctagcagtga gcagttgaaa gagattttca aggcggccca agatttacaa agagaaattc
    28441 aagagaaaca aattccaaat atgcctatga ccgagttaag tatgggaatg agtcgtgatt
    28501 ataaagaagc gattcaattc ggttccactt ttgttcgtat aggtacatca ttttttaagt
    28561 aggagagaac catgtcttta aaagatagat tcgatagatt tatagattat tttacggagg
    28621 atgaggattc aagtctccct tatgaaaaaa gagatgagcc tgtgtttact ccagtaaatt
    28681 cttcacagga accggctctc ccaatgaatc aaccttcaca gtcggctggt acaaaagaga
    28741 acaatatcac cagacttcat gcaagacaac aggaattggc aaatcagagt cagcgtgcaa
    28801 cggataaggt cattatagat gttcgttatc ctagaaaata tgaggatgca acagaaattg
    28861 ttgatttatt ggcaggaaac gaaagtatct tgattgattt tcagtatatg acagaggtgc
    28921 aggctcgtcg ttgtttggac tatttggatg gagcttgtca tgttttagct ggaaatttga
    28981 aaaaggtagc ttctaccatg tatttgttga caccagtgaa tgttattgta aatgttgaag
    29041 atatccgttt accagatgaa gatcaacagg gtgagttcgg ttttgatatg aagcgaaata
    29101 gagtacgata atgatttttt taattcgtat gatttataat gcagtggata tttactccct
    29161 gattttggta gccttcgctg tcatgtcttg gtttccaggt gcctacgaat ccagtttagg
    29221 tcgttggatt gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc tgcctttaca
    29281 gatagcgggt cttgatttat ctgtttgggt tgcgattgtt ttggttcgat ttttaggaga
    29341 aaacctagtg cgttttctgg cgatgatagg atgaataaag ggatttatca gcatttctcc
    29401 atagaagatc gtccatttct tgacaaggga atggaatgga taaagaaggt agaagatagc
    29461 tatgctcctt ttttaactcc ttttatcaat cctcatcagg agaagctatt aaagattttg
    29521 gccaaaacct atggtcttgc ttgtagcagt agtggggaat tcgtctcgag tgagtatgtt
    29581 cgagttttat tatacccaga ttatttccaa ccagagtttt cagattttga aatatctctc
    29641 caggaaattg tgtattccaa taaatttgaa tatttaacgc atgctaagat tttagggaca
    29701 gtcatcaatc aattagggat tgaacggaaa ctttttggag atatcctagt agatgaagaa
    29761 cgggcgcaga ttatgattaa tcagcagttt cttcttctct ttcaagatgg actaaagaaa
    29821 attggtcgta tacctgtttc gctggaggaa cgtcctttca ccgagaaaat agataagcta
    29881 gaacagtatc gagaactgga tttatctgtg tctagttttc gattagatgt tcttttatca
    29941 aatgttttga aactatctag gaatcaagca aaccagttga ttgaaaagaa acttgtccaa
    30001 gtaaattatc atgtggtaga caaatcagat tacactgttc aagttggaga cttgattagt
    30061 gtgagaaaat ttggtcgctt gagattactt caagataagg gacaaacgaa aaaagagaag
    30121 aaaaaaataa ccgtccagtt attattaagt aagtgaggaa tagaatgcca attacatcat
    30181 tagaaataaa ggacaagact tttggaactc gattcagagg ttttgatcca gaagaagtcg
    30241 atgaattttt agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg aatcatgata
    30301 aaaatttgcg tattaagagt ttagaagagc gtttgtctta ctttgatgaa ataaaagatt
    30361 cattgagcca gtctgtattg attgcccagg atacagctga gcgagtgaaa caggcggcgc
    30421 atgaacgttc aaacaatatc attcatcaag cagagcaaga tgcgcaacgc ttgttggaag
    30481 aagctaaata taaggcaaac gagattcttc gtcaagcaac tgataatgct aagaaagtcg
    30541 ctgttgaaac agaagaattg aagaacaaga gccgtgtctt ccaccaacgt ctcaaatcta
    30601 caattgagag tcagttggct attgttgaat cttcagattg ggaagatatt ctccgtccaa
    30661 cagctactta tcttcaaacc agtgatgaag cctttaaaga agtggttagc gaagtacttg
    30721 gagaaccgat tccagttcca attgaagaag aaccaattga tatgacacgt cagttctctc
    30781 aagcagaaat ggaagaatta caagctcgta ttgaggtagc cgataaagaa ttgtctgaat
    30841 ttgaagctca gattaaacag gaagtggaag ctccaactcc tgtagtgagt cctcaagttg
    30901 aagaagagcc tctgctcatc cagttggccc aatgtatgaa gaaccagaag tagctccagt
    30961 gcatccgaca ggtccaacac cagctacaga aactgttgat tcagcaccgg gatttgaagc
    31021 accgcaagaa tctgttacaa ttttataaga aatattctga gaacaatatc ttatccttat
    31081 attcccagcg agcaggagat ggtgtgagtc ctgcattccc tatcgataag attatcctct
    31141 caaaaactca agtctgaatc tagtaagatt tgacgttccc cacgttacgg gataagaggg
    31201 agaaagacta aaactttttc cgaataaagg tggtaccacg attttcgtcc tttttggaag
    31261 tcgtggtttt taatttgtta ttatttataa aggagatacc atgaaactca aagacaccct
    31321 taatcttggg aaaactgaat tcccaatgcg tgcaggcctt cctaccaaag agccagtttg
    31381 gcaaaaggaa tgggaagatg caaaacttta tcaacgtcgt caagaattga accaaggaaa
    31441 acctcatttc accttgcatg atggccctcc atacgctaac ggaaatatcc acgttggaca
    31501 tgctatgaac aagatttcaa aagatattat tgttcgttct aagtctatgt caggatttta
    31561 cgcaccattt attcctggtt gggatactca tggtctgcca atcgagcaag tattgtcaaa
    31621 acaaggtgtc aaacgtaaag aaatggactt ggttgagtac ttgaaacttt gccgtgagta
    31681 cgctctttct caagtagata aacaacgtga agattttaaa cgtttgggtg tttctggtga
    31741 ctgggaaaat ccatatgtga ccttgactcc tgactatgaa gcagctcaaa ttcgtgtatt
    31801 tggtgagatg gctaataagg gttatatcta ccgtggtgcc aagccagttt actggtcatg
    31861 gtcatctgag tcagcccttg ctgaagcaga gattgaatac catgacttgg tttcaacttc
    31921 cctttactat gccaacaagg taaaagatgg caaaggagtt ctagatacag atacttatat
    31981 cgttgtctgg acaacgactc catttaccat cacagcttct cgtggtttga cggttggtgc
    32041 agatattgat tacgttttgg ttcaacctgc tggtgaagct cgtaagtttg tcgttgctgc
    32101 tgaattattg actagcttgt ctgagaaatt tggctgggct gatgttcaag ttttggaaac
    32161 ttaccgtggc caagaactca accacatcgt aacagaacac ccatgggata cagctgtaga
    32221 agagttggta attcttggtg accacgttac gactgactct ggtacaggta ttgtccatac
    32281 agcccctggt tttggtgagg acgattacaa tgttggtatt gctaataatc ttgaagtcgc
    32341 agtgactgtt gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat ttgaaggtca
    32401 attctatgaa aaggtagttc caactgttat tgaaaaactt ggtaacctcc ttcttgccca
    32461 agaagaaatc tctcactcat atccatttga ctggcgtact aagaaaccaa tcatctggcg
    32521 tgcagttcca caatggtttg cctcagtttc taaattccgt caagaaatct tggacgaaat
    32581 tgaaaaagtg aaattccact cagaatgggg taaagtccgt ctttacaata tgatccgtga
    32641 ccgtggtgac tgggttatct ctcgtcaacg tgcttggggt gttccacttc ctatcttcta
    32701 cgctgaagat ggtacagcta tcatggtagc tgaaactatt gaacacgtag ctcaactttt
    32761 tgaagaacat ggttcaagca tttggtggga acgtgatgcc aaagacctct tgccagaagg
    32821 atttactcat ccaggttcac caaacggcga gttcaaaaaa gaaactgata tcatggacgt
    32881 ttggtttgac tcaggttcat catggaatgg agtggtggta aaccgtcctg aattgactta
    32941 cccagccgac ctttacctag aaggttctga ccaataccgt ggttggttta actcatcact
    33001 tatcacatct gttgccaacc atggcgtagc accttacaaa caaatcttgt cacaaggttt
    33061 tgcccttgat ggtaaaggtg agaagatgtc taaatctctt ggaaatacca ttgctccaag
    33121 cgatgttgaa aaacaattcg gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc
    33181 aagcaatgac gtgcgtatct ctatggatat tttaagccaa gtttctgaaa cttaccgtaa
    33241 gattcgtaac actcttcgtt tcttgattgc caatacatct gactttaacc cagctcaaga
    33301 tacagtcgct tacgatgagc ttcgttcagt tgataagtac atgacgattc gctttaacca
    33361 gcttgtcaag accattcgtg atgcctatgc agactttgaa ttcttgacga tctacaaggc
    33421 cttggtgaac tttatcaacg ttgacttgtc agccttctac cttgattttg ccaaagatgt
    33481 tgtttacatt gaaggtgcca aatcactgga acgccgtcaa atgcagactg tcttctatga
    33541 cattcttgtc aaaatcacca aactcttgac accaatcctt cctcacactg cggaagaaat
    33601 ttggtcatat cttgagtttg aaacagaaga cttcgtccaa ttgtcagaat taccagaagt
    33661 tcaaactttt gctaaccaag aagaaatctt ggatacatgg gcagccttca tggactttcg
    33721 tggacaagca caaaaagcct tggaagaagc tcgtaatgca aaagttatcg gtaaatcact
    33781 tgaagcacac ttgacagttt atccaaatga agtggtgaaa actctactcg aagcagtaaa
    33841 cagcaatgta gctcaacttt tgatcgtgtc tgagttgacc atcgcagaag gaccagctcc
    33901 ggaagctgcc cttagcttcg aagatgtagc cttcacagtt gaacgtgctg ctggtgaagt
    33961 atgtgaccgt tgccgtcgta ttgacccaac aacagcagaa cgcagctacc aggcagttat
    34021 ctgtgaccac tgtgcaagca tcgtagaaga aaactttgcg gaagcagtcg cagaaggatt
    34081 tgaagagaaa taagattgaa aagtctaggc aaaattcaat ttgagaagaa aagacaacta
    34141 attttatagt ctattaaacg cattgtatca cgtttttgaa cacctgatac gatgcgtttt
    34201 tatttatttt gaaaatttgt gaggtgtgac tttttatact caacaagaat caaagagaaa
    34261 cttagcaagc taacagtagt aagataaaat aggaatttga ta
//
LOCUS       DAVLTM010000008        32949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLTM010000008 DAVLTM010000000
VERSION     DAVLTM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.008"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(492..2219)
                     /gene="ezrA"
                     /locus_tag="VYN16_000333"
     CDS             complement(492..2219)
                     /gene="ezrA"
                     /locus_tag="VYN16_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1402922.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2301..4247)
                     /gene="gyrB"
                     /locus_tag="VYN16_000334"
     CDS             complement(2301..4247)
                     /gene="gyrB"
                     /locus_tag="VYN16_000334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1402923.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4262..4825)
                     /locus_tag="VYN16_000335"
     CDS             complement(4262..4825)
                     /locus_tag="VYN16_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1402924.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLVLYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4910..5464)
                     /locus_tag="VYN16_000336"
     CDS             complement(4910..5464)
                     /locus_tag="VYN16_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1402925.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5475..6698)
                     /locus_tag="VYN16_000337"
     CDS             complement(5475..6698)
                     /locus_tag="VYN16_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1402926.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6735..9185)
                     /locus_tag="VYN16_000338"
     CDS             complement(6735..9185)
                     /locus_tag="VYN16_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1402927.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9385..10314)
                     /locus_tag="VYN16_000339"
     CDS             complement(9385..10314)
                     /locus_tag="VYN16_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1402928.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDLLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10454..10741
                     /locus_tag="VYN16_000340"
     CDS             10454..10741
                     /locus_tag="VYN16_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1402929.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10775..12109)
                     /gene="ciaH"
                     /locus_tag="VYN16_000341"
     CDS             complement(10775..12109)
                     /gene="ciaH"
                     /locus_tag="VYN16_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1402930.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12099..12773)
                     /gene="ciaR"
                     /locus_tag="VYN16_000342"
     CDS             complement(12099..12773)
                     /gene="ciaR"
                     /locus_tag="VYN16_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1402931.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12882..15428)
                     /locus_tag="VYN16_000343"
     CDS             complement(12882..15428)
                     /locus_tag="VYN16_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1402932.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15551..15991)
                     /locus_tag="VYN16_000344"
     CDS             complement(15551..15991)
                     /locus_tag="VYN16_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1402933.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15966..16835)
                     /locus_tag="VYN16_000345"
     CDS             complement(15966..16835)
                     /locus_tag="VYN16_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1402934.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQEFVKDYLDTICSFYLGKEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLDRILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16813..17313)
                     /locus_tag="VYN16_000346"
     CDS             complement(16813..17313)
                     /locus_tag="VYN16_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1402935.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVISYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17324..18022)
                     /locus_tag="VYN16_000347"
     CDS             complement(17324..18022)
                     /locus_tag="VYN16_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1402936.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGFQVYGVDQGENPLLEGDF
                     HFLQRDLTLDLEPIFGWCPQVDVLCNTAGVLDDYKPLLEQTAQEIQEIFEINYMTPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18015..18251)
                     /locus_tag="VYN16_000348"
     CDS             complement(18015..18251)
                     /locus_tag="VYN16_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1402937.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18351..19277)
                     /locus_tag="VYN16_000349"
     CDS             complement(18351..19277)
                     /locus_tag="VYN16_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1402938.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19352..20167)
                     /locus_tag="VYN16_000350"
     CDS             complement(19352..20167)
                     /locus_tag="VYN16_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402939.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LHYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFRGFKKIKWSEV"
     gene            complement(20171..20491)
                     /locus_tag="VYN16_000351"
     CDS             complement(20171..20491)
                     /locus_tag="VYN16_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1402940.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20743..20823)
                     /locus_tag="VYN16_000352"
                     /pseudo
     CDS             complement(20743..20823)
                     /locus_tag="VYN16_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20867..22864)
                     /gene="metG"
                     /locus_tag="VYN16_000353"
     CDS             complement(20867..22864)
                     /gene="metG"
                     /locus_tag="VYN16_000353"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1402941.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22988..24247)
                     /locus_tag="VYN16_000354"
     CDS             complement(22988..24247)
                     /locus_tag="VYN16_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1402942.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKFQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24249..24950)
                     /locus_tag="VYN16_000355"
     CDS             complement(24249..24950)
                     /locus_tag="VYN16_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402943.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24934..26133)
                     /locus_tag="VYN16_000356"
     CDS             complement(24934..26133)
                     /locus_tag="VYN16_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1402944.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26398..27744
                     /gene="gor"
                     /locus_tag="VYN16_000357"
     CDS             26398..27744
                     /gene="gor"
                     /locus_tag="VYN16_000357"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1402945.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27941..28477
                     /locus_tag="VYN16_000358"
     CDS             27941..28477
                     /locus_tag="VYN16_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1402946.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28533..28613
                     /locus_tag="VYN16_000359"
                     /pseudo
     CDS             28533..28613
                     /locus_tag="VYN16_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28682..28897)
                     /locus_tag="VYN16_000360"
     CDS             complement(28682..28897)
                     /locus_tag="VYN16_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1402947.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PNHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28995..29315
                     /locus_tag="VYN16_000361"
     CDS             28995..29315
                     /locus_tag="VYN16_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402948.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29346..29684)
                     /locus_tag="VYN16_000362"
     CDS             complement(29346..29684)
                     /locus_tag="VYN16_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402949.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29696..30415)
                     /gene="trmD"
                     /locus_tag="VYN16_000363"
     CDS             complement(29696..30415)
                     /gene="trmD"
                     /locus_tag="VYN16_000363"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1402950.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30405..30923)
                     /gene="rimM"
                     /locus_tag="VYN16_000364"
     CDS             complement(30405..30923)
                     /gene="rimM"
                     /locus_tag="VYN16_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1402951.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30954..31742)
                     /locus_tag="VYN16_000365"
     CDS             complement(30954..31742)
                     /locus_tag="VYN16_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1402952.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDEYGPELYLKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32319..32558)
                     /locus_tag="VYN16_000366"
     CDS             complement(32319..32558)
                     /locus_tag="VYN16_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1402953.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32578..32850)
                     /gene="rpsP"
                     /locus_tag="VYN16_000367"
     CDS             complement(32578..32850)
                     /gene="rpsP"
                     /locus_tag="VYN16_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1402954.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
BASE COUNT         9743 a         7241 c         5895 g        10070 t
ORIGIN      
        1 cgagaaatat ctatcctatc ttctagaatg tcttccaaac gagaaaactc tcgtaaacaa
       61 agaggtttta gaggcctatt taccgtggga ctaaagttgt acaagaaaag tgcaaataag
      121 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatagactt
      181 cgttattgga cggttaccaa gttactcaaa gtacagattt gaagctgcag ataagactga
      241 cgaaatcact ttaatactaa atgaaaatca aaaagcaaac taagaagcta gcctcaggct
      301 gtacttgagt acggtaaggc gacgctgacg tgatttgaag agattttcga agagtataaa
      361 acactgtttt gaggtggtgg ataaatcggc tgggtaaaaa ctcaatctca ggagaaaatg
      421 aagtaaatct ccccacaact atagaaaaag atttgattct gctcctcaca caataaaatc
      481 tttttctttt attaaaaacg aatcgtttca cgtgttttct catatgaggt aacaaagcga
      541 ttggttacac caggctctgc cacttccaat gcttgagaaa tcttgtcaaa tgaagcgtga
      601 taatcaaatt ctttttcaaa aatatctaaa gcttcgttaa atgcttcttg aatgcgttca
      661 tcaaatgagc gatagcggtt agaatattgc aagagttgct ctgtcaaagt tgcatattgt
      721 acaatattat aagtttccgt ttctaaagct tccatatcat tcgttgcaat ttcaagaact
      781 cgggtaacag attcaatgtt aatcattttt tgttctaact caaccattaa atcctcggta
      841 ttattgcttg ccgtaaagaa taacttcaag aaagtttgtg gaatacctgg cagattgcgt
      901 ttttccatgt atcgcttgat agtatggaga cgattgacat aaacattggc cttttgacgt
      961 gcattaatat catctttctc aatttgtgtc aggcgctcac taactgaaat ttgctcatct
     1021 tcaatatctt ttagttgagt ttgtaaatcc tcaagatttt cttcaagaac tgaataagct
     1081 tgggttggtt cttcttgatt cgaagttacc tcaacaatag ctgcctcaaa actctctaat
     1141 tctgtctgaa tacgacgaac atggcttgca gctgtctcag gaagtaaata ggtcttgttc
     1201 aaacgtgcaa tatcttctcc caataaagta ttattctctt tcatatgttg aaggtaagtt
     1261 ggaagagttg caagtagatt ttccactact ttctgagcag caatttctcg agtaaaaata
     1321 tcatacaagg cattgatttc ctcttgtgcc tgtccattct catattcagc attatccaat
     1381 tccaactgac gaatattctc ttggtttttc ttgaatgctt cataaagcaa gtggaaacgc
     1441 gcttcaatat ccgtttcaac aaaatgataa ttagcatcaa ttagtttacg ataaccggct
     1501 tccaaatcct gtaattgatc tggcaattct gtagaaagcg tcgtaaccaa ggctggaaca
     1561 cgatccacaa tatgacttaa ggccaaaatg tgattttctg tattatccaa aatcactgcg
     1621 gcttccacag ggtcacccga tgaattcaag gttacaaatt gtgaaaattc agattggata
     1681 ttttctaatt gtttttcaat ttcatccaag gcttgaccat actgttctga attttcagca
     1741 actctatgct gaagttcctc aaataaatcc aaagcatgaa gaacacgacc actattttta
     1801 gattcttgct tctctaagtc tgccaaagca ttgcgaattg ccgcaatatc ttcttcaatc
     1861 aaagtaattt gactctcaat ttggtcaatt tgatgactgg ccttgagaaa acgaaatgaa
     1921 tggttatagc cttctgcttc aaagagatta ttttcaatat cggcaaaaga gttgagagat
     1981 aaatcgaccc atttttgatt ccattcacgg aaagctactt gactttgtcc aatcaagtgc
     2041 atatttttta cagcttctac ttcatcattt actggaagat tgtatagttc ttcttttctt
     2101 tcttctagcg cctctaatct cccctcgttt cgcttacgta gaaagattgc cactacataa
     2161 gccagaacta aaatgactgc aattgcaacc attaaataaa ttagttgtcc attagacata
     2221 tcaaactcct tttttacttg aaacaatcgt aacgattata tcatattttt tagaccaagg
     2281 gaactacttc tccctatttt ttagacatca agtgtactat agacagcatt ttcttcgata
     2341 aactcacgac gaggctctac tcgatccccc atcaacatat caaagatttt atctgcttct
     2401 gcagcatcat ctacagaaac tctagccatc aagcgatgtt cgggatccat ggttgtttcc
     2461 cacagctgat gatcgtccat ttcacctagc cccttataac gctgaatagt cggtttggta
     2521 cgaccttcac tataacgggc taaagcttct tggagtttga tttcttgatc tgcacccggc
     2581 tggatatatt ctttaatctc gcttccaacc ttgacaccat agattggtgg ttgggcaata
     2641 taaacatatc cagcttctag gattggtttc atataacgat aaatcaaggt taaaagaagg
     2701 gtacgaatgt gggctccatc gacatcggca tcggtcatca aaacgagttt ttggtaacgg
     2761 gctttcgaaa catcaaattc tgcgccaaat cctgttccca tggctgtgaa aagactacga
     2821 atttcttcgt tggctagaat cttatccata cttgcttttt caacattcaa aatcttaccg
     2881 cgaattggaa ggatagcctg aaactcacgg ttacgaccag atttggctga tccaccagct
     2941 gagtctcctt cgacgatgaa gagttctgtt tcagcagggt tattagaaga acagtctgct
     3001 agtttccctg gaaggttgga aatttccaaa ccagattttt tacgtgtgac ttcacgcgca
     3061 cgcttggcag ccacacgagc cttggcagcc aaaattcctt tttctacgat acgtttggca
     3121 atctgtggat tttccatgag gaaatcggag aaggcttcac tgaagaggcg attggtaatc
     3181 ttgaccactt cgctatttcc caatttggtc ttggtttgtc cttcaaactg tggatttggg
     3241 tgtttaactg agataactgc agttaagcct tcgcgaacat cttcccctgt taaattatct
     3301 tcattgtctt tcagtaactt attcttacga gcataatcat tgataacacg agtcagtgcc
     3361 gtacggaaac cttgttcatg cgttccacct tcatgggtat gaatattatt ggcgaaactc
     3421 atgacatttt catggtaacc tgttgtgtac tgcatggcta cctcaactgt aatatcatcc
     3481 atctcaccgt ctgtatagat tggtgtatca aagattacat ccttgttctc gttgatatat
     3541 tcaacgtaac tagcaatccc accttcataa tgataatgct tggtttgttc caaaccttgg
     3601 cgcttatcag tgatagaaat ttgaagaccg cgatttagaa aggccaactc ttgaatccgt
     3661 ttatttaatt tatcaaaatc aaagattgtt gtttcagtga aaatttctgg gtccggtgtg
     3721 aagtgaaccg ttgttccagt tctatctgta tccccaacca cctcaaggtc tgcaacaaca
     3781 tgaccacgac ggtattcttg gtaatgaatc ttaccgtttt tatggacatg aacgtctaat
     3841 tgagtggaaa gggcattaac tactgacgac cccaccccgt gaaggccacc tgaaaccttg
     3901 tatccaccac cgccgaactt tcctccagca tgaaggactg taaagacggt ctcaacagca
     3961 ggacggcctg ttttttcctg aatatcgact gggataccac gcccatcatc cacaacagta
     4021 atcgaatcat ctggctcaat aaaaacttga atatggctgg caaatcctgc caaggcctcg
     4081 tcaattgagt tatcaacaat ttcccagact agatggtgaa gaccttcttt tgaggttgat
     4141 ccaatgtaca tccctggacg catacgaaca gcctctaagc cctctaaaac ttgaatttga
     4201 ctggcatcat aatcctgtgc ctgcagattt ttgatttctt ctgtcatctt attccttttt
     4261 cttatatgtc taatacttgt cttaaattca cgatactggt aaacaagtgg gtatcaagtc
     4321 ctgcagcttg acctgcttcg atatcaatcg gccgatcacc aatgacaaga ccagagctaa
     4381 tctgatactt ttctcttaaa taaagcatgg attcgggatt tggctttctc ttaaagcctg
     4441 agctagaagt caccacttct gtaaaataag ctgctataga ggttttttct aaaatttcca
     4501 aaacctgatc atttcgatga gagaccaaaa aatgacggcc accttgattt gaaatgactt
     4561 ccaataggtc agaaactcct tcaaataaaa tcgggtgttc aagctctctg gcttcatttt
     4621 ccttgtactt ttctaaaaaa ttctctaaat tgggagcgaa tgtctcaatc gcaaaaggag
     4681 tagaaacctt taaagcttga tagacactgt catggtcttg tgtgatacca tacagtacca
     4741 atgtttcaac aaatgcagct gttgaagttt cataattatc cagtaaagtt ccacctaaat
     4801 cccagatgta atcgtgatat ttcataccct tcattatagc atttttttat gaaaaaagcg
     4861 atgatttccc tgagttttcc acataaaaaa cgagagaata actcccgttt tactgatttt
     4921 taccaaagac atctcgatag agcattcctg ttcgtaaact ctctgcgtcc cctcctagtt
     4981 gctccaccaa ctcgtaggca aaggcaaggg ctgttgaagg accccgactg gttgtcaact
     5041 gaccatctac cactactgtt tccttgacgt agtgaccatc aaggatttgc tcttgaacgc
     5101 cgtcataaca agtgtatcgc ttatttttca atatctctgc ttgattgagg gcaattggtg
     5161 ccgcacaaat ggctgctagt ttcttccctt cttgctcgaa gctttgcaat tcttgaatca
     5221 aggtctgatt atcacgtaaa tgtgcagaac caggcatacc tccaggaaga acaatcatat
     5281 catagtctga taaatttcca tcaaagacat gatctgctct tacttggatt gcatgcgaac
     5341 ccgttacttg ctcttcaaaa ccaaccatat cacatgtgat attggctcga cgcaagacat
     5401 ctacaactgt caaggcttca atttcttcaa agccctgagc taacataact gctactttta
     5461 ccatgatttt ctccttattt aatttgtttt aatacaacct ttttccgttt gaatgggact
     5521 tttccgctct tttcttcagc tagattagtc tggtaactca tcgataaaag caaaccaaca
     5581 ccaatcagat tactgataat agctgatccc ccttgcgaaa tgaaaggcaa gggaatcccc
     5641 gtcaaaggaa gtagtccagt cacagcaccg atattctcaa agatgtggaa gagcaacatc
     5701 ataatcaaac ctgtggaaat ataagtgtag aactggttat ttgatttaag agtaatcttc
     5761 aacatacggt aaatcaacat gagatagagg gcaataacca ggacagagcc aataaagcca
     5821 aaatcttctg caataaccgt aaaaatcata tctgactctc gaactgggat aagcagattc
     5881 gaagcattaa atccctgacc aaataagcca ccactcccaa tggcaatctg cccttgagcc
     5941 tgctggtaag tcgttgtttg ggcaaactca aagggattga gccaagccaa aatccgattg
     6001 atttggtagg tcggcattcc aatctggtga agaaaagctc gtccgtcctt gctaataaag
     6061 atagctaaga aaccagcaac tcctgttaca gcagtcacaa atactgggat aataattttc
     6121 caagaaaccc ctgataataa aacgattcct gagaaaatgg ctacaaaaac caaagccgtc
     6181 cccaagtcac tttgaagtgc taaaagaact aggactggaa tggtaaagag aatcatccag
     6241 aaaattaaca aaaagtccag cgaaaccgtg cgtctccatt ccttatgttt ctttgtaaat
     6301 tggacaatga cacgagccaa catgaggata taggatatct tcataaattc tgacggttga
     6361 aatagggtaa ttccatttat tgatacccag tttttggcac ccgttgatgc aactaagctt
     6421 ggattataaa atacaatcgg caagatcata agtcccaagc ctaaaatata tagaaagggg
     6481 gtcaccttcc aaagaaattc tgtattaaag agcatgacca caaaaccaat cacaagcccc
     6541 aaggcaatcc aggcgacctg ctgccctaaa atgggcagaa tattattggg ataatcatga
     6601 ctaacggcta tatagatagc caccacaccg atgaccagta gaaaaaatac tggcaagagc
     6661 aaactgtaat cgactcttga gtcgagagaa cgtttcatat atactaacct tatactttca
     6721 tacaatacta tttatcaaag ttcattaaaa aatctatcaa tagcctcgtc aacttcggat
     6781 cgagagatgg ttttaacagt cgcttcttct gctagagatg ctactatttg tttgccgtat
     6841 cgttttccga cgattctcct atccaaaata agagttaagg aacgttggta ttcacgtctc
     6901 atacttcttc ccaaagcctg ttttaaacga ataatggcca ttggcaattg ataatcataa
     6961 aaggcatttt tcccttcttg attcagttct tgattaatct ttttcgtcaa gggttcttga
     7021 ggattttgga aaggaagcct cggtacaact tgaatcacaa aaggatggct tgaaaaatca
     7081 actccctccc agaaacttgc tgcaccaagc aagatttgtt gttcaccttt ttcaaagcgt
     7141 ttctttagct gatgaacatc cccattttta tactgggcca agtggctaac tgtaagtaaa
     7201 tccgatactg ctagaagcat gtctttagcg gtaaagagaa ccaaaatcgg ttgctggaaa
     7261 gcttgaattt ccactagtaa agcagctacc tctctggcat agacttctaa ggaggtttct
     7321 gttaccagag ggaaatcttt gaccataacc acttcttgtt cctgttttcc ccgagattca
     7381 atcttgacaa atttggcttc aggatagcct aaaaggtctg ccaaagaaac cctctgacta
     7441 atctcaagag tagccgatac tcccaagact tggcaactct caggaagtaa agaggacaga
     7501 agagtacggc ctgattttgt agaagaaatc tgaattttct tttgactcgt ttcagttact
     7561 tcaagccagt aatcaccttc agctgtaaaa tagcgaacca gctcatcaaa gccttctact
     7621 tctaattctg aaaaatactg atggagattg tccagagaat ctaagatatt tttcctagat
     7681 ttaccagact gaaattgttc tatcaagtag agacactcaa agcgaatact ttctagtatc
     7741 cgttgttgaa ccctgttttc ttcccctact aaagccttat caattaaatc gataatagat
     7801 tgtatatcgt aggtctcttg aagcagattt tctagagcta acaaaatctt ttggacttca
     7861 tcaataatca gtaaacggtc actgacaaat tcaggattat cttcaagtct ggtcacgaga
     7921 taggcatgat tagtcactaa aagcttgcaa gtctctgccc tttcttgact acgtttccaa
     7981 aaatcttccg tcacaaataa gctctgggat gataaattcc catcatgacg aaggtctgct
     8041 agaaaatgtt ggtaacggta gagttgcccg atttcatcca aatctcctgt ctctgtctca
     8101 gtaagccaga ccaagacttg cattttaaag cgtctaaata agcgattttc atcattttcc
     8161 tgcaaggaat gataaaaggc atccaacttc agataatttt gtggtccctt taagctatga
     8221 atatctgtat ggaacacttc cttgagacgt ttaccttctt cttccatgat ttgattttga
     8281 agaatctttg tcggaacact aagaacaatt tgtcgctctt tggattgaga taaagcgggt
     8341 aagagatagc cataggtttt cccaatccct gtcggcgctt gaatcagaga gacaggttca
     8401 tctttcaata gcaagccaac ctctttagca aaactttctt gttcctccct cacttcaagg
     8461 ttcaacagag aaatattttt agaaaagtct tgagatagtt ttcgtagctc cagagaagct
     8521 gccgttttct taaaatacag accttgaact tggaccaagt ctggagaact caggatagat
     8581 tggttgcgat aagtttcctc aataaccagg taggactcat ataggagagc gtcagccatt
     8641 tccagcaagc gttccaagag acctttagga agctgggtca tcttttcccg taaaaaaaga
     8701 agtaattctg ctgtagcttg ggcatctgaa agggctgtgt gtgcgtgttt aagaggaatt
     8761 cctaattctc gacacaaaat cggcaagcta tatttttcca gttcagggaa aaagacctgg
     8821 gccaattcga ccgtatcaac acgagggttt cttagctcat agccttcaaa aaataaattt
     8881 tccgccaaga gattagcatc aaactgaaca ttatgggcta caaaaatccc atcctccacc
     8941 aagtcaaata tttttctggc aacttgcgaa aaatcaggtg cttgcgccag acgttggtct
     9001 gtcaatcctg tcagttcttt gatatgagca tccaagggtt catgtggatt gacatccgtc
     9061 gtatagtgat cgacgatttc tccgtcctca atcacgacaa ttcccacttg gataatttta
     9121 gccttactac ctgtgctagt tgcctctaaa tccaccacaa catagcgatt accaatcgtt
     9181 ttcattataa aaaattatac caaaaaaagg gccatttggc acctgcaaac ataggataat
     9241 tttcaaactc aagaacacta cttttcttaa aaatcccatc gaatagagca ttctagcctg
     9301 ctgatttttt taaaatagtg aagaaatacg aaaaattgag agaagaaatg ttttcatctt
     9361 ccttctctca acgaatcatg tattttattt aaccatgtca ggatcgtagt cataaaatcc
     9421 tgtatcaagg aaacggtttt tagggtgaag tttacgaact tcttcatcca gcaagaaggc
     9481 ttggtcatgg ctaaagttgc cctcttgaat caagctacgc gcttggataa agagttttgc
     9541 aatctcaaca aagttctgac caggagtgta gagcccttct agtttccagc gagtaaagcc
     9601 atgctccacc aattctgtta atttgatcat caaatcaagg tcattgttgg caaagatatg
     9661 ggtcccatga ttatcttcaa aaatggaata gtggctctct ggatcacttg gctcagccaa
     9721 gaagaggtca cgtttatgcg tcttttcatc atcgatatgt gtaaagttat agtagttttg
     9781 caagagcgga cgtttagaat gatggatgac actagcaccg taaaccaaaa cttcagcagg
     9841 aatttccaaa atctctggca ttttgaaaag ttcagctgat ggaatttcac gcgccaaaac
     9901 agcctcagat gcaccagcct tttgtcccca gaagttaatc tgacgactgc tagttaccat
     9961 ggttgaagca tcgtagatgg tcttaaatga ataaccatcg cggttgacta cgtaaaagac
    10021 gcctgcatcc ccaatcgtaa tatagtctgt cttgatttct tccaataagt ctaagaaagg
    10081 cttgatacgg tccatcatat cttggtgcat gagagcattg accgcaacga tcaattcctt
    10141 accagcatca tgaaccaact tagcgatttc acgtaattgg tcataactaa aggtcgttgg
    10201 cagacgaagg ccaaaatctt tctcaccgac atagatacgg tctacgccag cttcgagtag
    10261 ttgttcaact tgttcaatac tttcagcagt tgctgtaatg acaatctttt ccataagaaa
    10321 aattatacca catttctcca aaatcagcca tgctttgaaa atgaaaaggg actttattct
    10381 ttttgtaacc tttctgtaat aattctctgc cgaaaaaatg ataaaatagg tatgtactgt
    10441 taaggagaga atcatgcccg taagaaaatt acaatcctat gaggtagact atcaagaaga
    10501 attaaaccag cagcttcctc attaccaagc ttatacacct gaagcgcaat ctgatgccaa
    10561 tctcaaggaa atcctatttt ttattaatat cgctgttttt tgtatctgta ttgctatctt
    10621 tagttttatc tttttagcat taaaattatc aactgctctt gcctttgccg cagcaatcgg
    10681 attcagctta cttgttttaa aagttcaacg gtctattatc aaacgaaaac gtagaagata
    10741 aatccaaaat atcgccccaa ttggagcgat atttttattt tttcttttta gatggtgtct
    10801 gaatggcaat cttcacttca aagattgttc ccttgggttt attatctttg acagtaacag
    10861 ttccttttag agcatctaca atttgcttgg ctagggataa tcctaaacca aaaccacctt
    10921 tttgccgggt tctagccttg tctactcgat aaaaacggtc aaaaattttc tttttatctt
    10981 ctgtcgaaat accgattcca ttatcagaaa caagtaaata aagattgcga tcggtcgccg
    11041 agataagaaa atcaatttca ccatcctcct cagtatactt gacggcatta tcgaaaagaa
    11101 tggtcatcag ttgtttcaga agaagctgat ctgtgacaat tgttcgatgg atacgatttt
    11161 caaaacggaa gacacgatta ttttccgaag caatcatctc atagtttgtg aaagttgtat
    11221 taaaaaagct agttggaact tctgcaagct ccggcttaat cccatcatct ctccgagcta
    11281 agttcagcaa gcttgtcgtt aaaaaacgca tatttcggac ttcttccaaa ctcgatgcaa
    11341 tgctttcgct cacatccata atggtagctt ctggcttacg aaaaagggtc tctaagcgat
    11401 tttgcaaaac tgcgagtgga gttcgtaact catgactggc attttccaca aaagactgtt
    11461 gcttctgcat actctcaagc aggggcctaa cactgaccct agctagatag agactggcaa
    11521 gtaaagacaa aatccagaaa ctagccatca cgaccacaat caattgctca tgcttttgac
    11581 tagcctgctc caactgactg gtattaatca agacagcagc atacttgata ttggttgaaa
    11641 ccgaactaat attggtctcc atcaaaatca cacgatagat ttcttcctgc ccatagctat
    11701 taaaaacctg aatctggtag atatgtccta gttctttctt ttctaactta atcttatcca
    11761 agcccaaaaa tcgatttcca gaaagaagtt gagtaaagtc cttatcaaag agaataactt
    11821 ccgtattgga actgacatta ggttttattt ctactttact agcgtccgcc ctagcatttt
    11881 ctaaatcttt aatctcttct gttgccctat ttatagccag ctgaataact gcttgaggat
    11941 tttcactcag tccatgaagc ttatcgtcca ccgaagtata aagactcgaa tgcatgactt
    12001 gtaaaataat cagagtcatt gtagaaaaaa tcagggtgaa gacaccgaag ttgcggataa
    12061 aataactaaa gtcatccgca taccatgttt ttttaagttt actgaacatc ttttaaaaga
    12121 tacccaacac tacgcaaggt ttgcaaattc tctgcaaaag tggttccctt taatttctta
    12181 cggacttttg aaacatagac ttcgacaacc gaaatcgttg tatcactatc aaatccccat
    12241 agacggtcaa aaatctgcgt cttaggcaaa atcacatttt gattttgaag gaaataaact
    12301 agtaaatcga actctttccc cagcaattcg acaggagtat cttcaacttt aacggtattg
    12361 gttgataaat taaccacgat attcccataa gtcaaggtgt tttcattaaa cttccctgaa
    12421 cgtttgagaa gggcctgaat ccgcatttta agttcttcta ggtagaaagg tttggtcaga
    12481 taatcatccg ctcccagttc aaatccatgt cccttgtcat ccaaactttc cttggcagtc
    12541 ataatcagaa ctggtgtcgt aattcccttt tcacgcaatt cttttaagac ttggaaacca
    12601 tttttttctg gcaacatcaa atccagcaaa atcaagtcat agacaccact ctcagcttcg
    12661 tagagacctt cttctccatc aaatacctgc ataacatccg caaaatcgtc taaaaagtca
    12721 aatactgaat ttgacagacc taggtcatcc tcaaccaata agatttttat catgagaaac
    12781 tcctccttat taaaactatt ataccaaatt tgccttaaaa aaaactcaac tctctgcatt
    12841 ttacatgaga tagctgagtt ttctttttat tttaggctta tttatgcatt tccgtattga
    12901 agaacaactg cttcgactgc agctttttca cggctaatca agtcaacacg tgctgcaatt
    12961 tccttgattc ccataccgat gttacggcta agagcaaggt cagaaagttg cggctcaaag
    13021 aactccttgt attccgccaa gcgttgctga gtcttaaata catgagcagg aaggataaca
    13081 aagctatcaa agctcatatc tcctccaagg gctgccttaa tccaagccca gttttcacgc
    13141 gcccaagacc aagctgtttt ctgagttgct tgatgagcta ggaattgata ataccaagca
    13201 gacaagtcct gtggtttgac cacaaatttg tccttccaag aagtaatcaa gttttggata
    13261 ttatccgcat ctgtactgta tgcaagagct gctgccaact ggcgtttaaa gacagcatct
    13321 gttgcgtgag tataagtatc aagataaagt gctaacaagt ctttagtctc atgatgtttc
    13381 atctcattaa tcagaacttg tgaacgaata gctgctggga gtcctgcaag attctccttg
    13441 tgtgttgcga agatttggct agcgacttga ctagcttctg catcatttga gcgaatcatc
    13501 atcgaaacag ccagctgacg aaccaattca tcctcatctg attctccgtc tttagcttca
    13561 aaaccaagac ggtcatagtt atgacgagcc aatttagcaa ccagtccttt gaaggctgtt
    13621 tcagcatccg ttccttcatc aataaagcgc tcaagggctg aaatcacttg agaaacagct
    13681 gaaaccacca gataagactc ttccttagca agtttatcaa gaactggaag caagtctgca
    13741 taagaaatgt gccctgcctc agccaacaaa cgacgttctt gaacaatttg cagtttgctt
    13801 gtgttatcaa gtgtctctag ctcagcaaga acagcttcta acaagtctcc ttgatagtcg
    13861 gtaatatagt gggcagtatt ttcagtgttg agacgaagag ctccttcatt ttcagcaaga
    13921 agagctgcgt agccagggat ttcgatactt tcagtttcga gtgtatcagg caagcctttc
    13981 cagttgctat tgaggggcac cacccagaga cggttcttgt cttcgttctc accgatgaag
    14041 aattgttttt gtgaaatctt caagacatca ttttcaactt tgacagtaag aactgggtaa
    14101 ccaggctgtt ccaaccaaga atccatgaag gctgcgacat cacgtcctga cgcttgacca
    14161 agggcatccc aaaggtcact accaatggtg ttgctgtatt ggtgtttttc aaagtaggcg
    14221 tgcaaacctt tagcaaaatc agcatctcct agccaacggc gaagcatgtg catgagacgg
    14281 cttcctttgg catagacgat agcgccgtca aagagtgtat tgatttcatc tggatgttta
    14341 acttcgacgt ggacagactg aacgccatca gtagcgtcac gttcaagagc aagaggtact
    14401 ccacctgttt ggaaatcttc aaagatattc cagcttggtt cgatggtatc cacacagacg
    14461 tattccatca tattagcgaa actttcattg agccaaaggt catcccacca tttcatagtc
    14521 acgaggttcc caaaccattg gtgagccaat tcatgggcca caacaagggc aacttgttga
    14581 cggctagcaa atgtagagtt ctcatcgaca accaagtaaa cttcacggta ggtcacaaga
    14641 ccccagtttt ccatagcacc agctgagaag tcaggaaggg cgatgtggag agattgagga
    14701 attgggtact taactccata gtaatcttcg taaaactcga tagagcgaac agcgatatcc
    14761 agtgagaaat caagatttga aagtggatgt gctttggttg agtagacacc taccagggta
    14821 ccatttttag ttttagcggt caccccttgc aaatcaccag caacaaaggc caacaagtaa
    14881 gaagacatgc gaggtgttgt ctcaaacttc cagatacctg tttccttacg gttttcaaca
    14941 tcgatttctg gcatgtttga caaggccaat tcaccttctg cttggtcaaa gcgaagagag
    15001 aggtcaaaag ttgctttggc ttcaggctca tccacacacg ggaaagcttc acgcgcaaaa
    15061 tggctctcga actgagtaga caagacctcc ttcttgactc catcaactgt ataataagaa
    15121 gggtaaatcc ctgtcatgtt gtctgtaatt ttaccagaaa aggcaagaac caattcaact
    15181 tgaccagcct cagccaattc gatatgaagg gcttcattgt catggtcaac tgtaaatgga
    15241 cgagcttgac ctgcaacttc tacagaggtg atttccaagt ctttttggtg gagggagatg
    15301 cggtcactct gtgcttgacc agtgatggtc actttcccag aaaaagtctt ggtctcacga
    15361 ctcaaatcta aaaataaatc ataatgttca ggaacaaatt gcttaataaa atgttcaact
    15421 gcttgcatag ttttctccta ttctaagttt aagagctgct gctcttcttc aaacaaactt
    15481 attatatcat attttgcttg agaaaaaagg attggacagc gatttccaaa actttcttcc
    15541 ttctaatagt ctacagtggg tagaccttgc gaaattcttc taaaacaacc ttgctttcag
    15601 gtgtaaaagt cagccctact tcacccaaac agtctgtgaa aaagtcctca tgaacctggc
    15661 gccaataggc aagtgatttg tctccctcac cttccttaaa ggcatgttgc gcagaaactt
    15721 gattgaagag ttcaacagaa acctttgtaa tttcgacaat gcagacagcc tgattttgac
    15781 tatctaaaat gacatcgaag gtccctactt ggggaagtgg ttcgtcttct agtacataga
    15841 ggtcataggc tgatgctgtt gctgtctttt ctcctttaaa caccaaatcc gctaaaaggt
    15901 ctggttcaac tccaaaagcc caagcatcga tttcatctcc gatcaaagga ttgatttgct
    15961 tgtatttatt ccacatttct tgcggtgtca tgggtactcc tttgtaattt tttactttct
    16021 tcttttatgt gtttaagatg atctggatgg tcaatctcta aatcaaaaat ctctggaata
    16081 gaactgtagt ggataatgca cttgataccc aactgattca ttttttgtat gaaagaagta
    16141 ttcagatagc ctgctacagc aaaattaatc ttgttctttc ttgctttatc ctgcatatct
    16201 cttagcatat ctaacattat tggactttcc atatcatgcc attgactgtt tctcatagtc
    16261 gcaaaaacaa aggaagtcaa atcattcatt ccaactacaa tctttgaaat gcccgtttcc
    16321 agtatcctat ccaagtcaaa atacgctgac ggtaattcaa tcatcgttcc gactttccca
    16381 gtaaagccat actgacgcaa tactgtaata gcttgtttta attggtcggc atcattgaca
    16441 aaaggaaaga taagagatag attgggattg gtttgataaa cttctgtaac gacatgtgct
    16501 tcagcctgaa attcatccaa acacgccagt aaacgcctgg ttcctctata gccaaacaag
    16561 ggatgccctt cgtcaaaaaa ctctctagtc cccactaaac aattggcttc tgtattcgtt
    16621 aattcagaaa aacgatacca aacttcctta cctaagtaaa aggagcaaat agtatcaaga
    16681 taatctttca caaattcctg acaacttgct aatatgatgt tttgattaag ctctctcaac
    16741 aaatattccc cacgaatcat gccgacgtgg tgaaatagtt gaggataaat tttttcaaga
    16801 attttttcgc cactaagggc aagttgattt ctcatcattc accttccaat tcatgtaaga
    16861 agtcttgtcc agttctggaa atcctaataa ttcagactta accttcaaga ctaatggcga
    16921 tgcattttct tctgtaatct cttgaatatc catccaaata tatccaagtg aatcattcgc
    16981 accatcagac acagcttccg aagtcgtaac ttgaggtgca ctctcattca tttcaacatc
    17041 atacaaggcc atgacatggt gaaccataaa attggttaac tcttctctga cgaaaacatc
    17101 gtagattcga ggattagagt agcttataac agtaaatccc gtctcttcca taacttctct
    17161 agtcagcgtt tccgtcagtc cttcaccaag ttgctgactg cctccaggta gatcataccg
    17221 atgttgataa gggcctctcg ctttttcaat gcagagtaac ttcccatttt ctaagcaaac
    17281 agcgtagact ccaaagtgtt ttttgatttc catccaactc ctcctacttc aaagaccagc
    17341 caccatctat tgtcaagatt tgtccttgca tggcagatgc ctttccactg gccaaaaaga
    17401 gactgacttc tgccacttcc tctggttcaa tccagcgctt gattggtgtt tcactagcca
    17461 cccagtcagc taagccacct ggctcaaaat ccgcagcggt catagccgtc ttgactgctc
    17521 ctggagcaat cccaaagacc tgaatcccag cttcagcata gtctagagcc aactgcttgg
    17581 tgaagccagc caaggcatgc ttggatgaag tataggcgtg accacctcca cctgctaggc
    17641 tagaagcaat ggaacacata ttgatgatga ttcccttttt attttccagc atttgtgtca
    17701 aataataccg agtcaactca acaggagtca tgtagttaat ttcaaaaatc tcttgaattt
    17761 cctgcgcagt ctgttccaac agtggtttgt aatcatccaa aactccagca gtattacaca
    17821 aaacatccac ctgagggcac cagccaaaaa taggttccaa gtccaaggtc aaatctctct
    17881 gtaaaaaatg gaaatcaccc tctaagagtg gattttcacc ttggtcaact ccataaactt
    17941 gaaagccctt ctctaaaaag agtcgagcct gagccaatcc gatacctgaa ctcactcctg
    18001 taataagtac acgtttagtc atgcacttct acccaatccg ttgccaaaac atcacaagct
    18061 gtcgggctcc acatggaaaa accttctcct tcgccagaaa cgttgattag gaaataaggt
    18121 gtcacttcaa gtgcaagccc gttttgttca atggtgtcaa aaagttggac atagttttcc
    18181 gcacctcccc aaccagttcg tacatatttt ttcttagcct ttaagccagg tagaatttct
    18241 tcaaatgtca tgtttttctc ctttaatgtc aataattctc cccttcatta taacaaaaaa
    18301 accgctttaa aacggctttt tgactgtgat ttatttacat cttcttctac ttacggcaaa
    18361 ttattccctg cagcaagata aatttcatac cattcttttc ttgttaagct aaagtttgcc
    18421 gctcggctaa cttctctcaa gtgcttagga tttgttgtac ctacgactgc ctgcattttt
    18481 gctggataac gcaatatcca agaaatggca atagttgaag aggttactcc atatttaata
    18541 gctaaacgat caagtacttg atttaaagct tgaaatttct catttccaac aaagttccct
    18601 ttaaaatacc cgaattgcaa gacagaccat gcttgaatga ccacatcgtg taatttgcaa
    18661 tattcaaaaa tgctgccatc tcgcatagct gcttgactat cttccatatt aacatgaaaa
    18721 gctgattcaa atcctggagt aaaagccgca ctcaattgta gctgattaac agctaacggc
    18781 tgcttgacat cttttttaag caactccatc atcataggat tttgattaga aactccaaaa
    18841 tctcgaacct taccttgttt ataaaggaga tcaaaggctt ctgctacttg gtcagattcc
    18901 atcaaagcat ctggtcgatg aaggagcaag ctatctagat gatcaatctt caatctttgc
    18961 aaaataccgt ctactgattt tataatatag tccttagaaa aatcaaaata ggtaaattct
    19021 tcaatgcgaa tgccacattt ggactgaatc cacatctttt ctcttaaatc tggacgattt
    19081 tttaggacaa gacctaacag ttcttcacaa cgaccacgac cataaatatc agccaagtcg
    19141 aaggcattga ttccaacaga aagtgctgtt tctacaagct cttcaacttc ttttacagac
    19201 ttatctttta ttctcatcat tccgagaaca atttctgata attctttgtc atcttgacca
    19261 agagttatgt atctcatcaa atttttctcc tttaatttct aacattcttc ccttcattat
    19321 aacaaaaaac cgctttgcaa cggctttttg actatacttc actccatttt atcttcttaa
    19381 acccacggaa caagagaaaa atgccaataa agaggacagc taaaggaata acttttgtaa
    19441 ggaaaacatt tgaaattccc atccactcat aataacggag cagagaaccc accacaagat
    19501 gggcaataat catactgaca aatggacgaa agaccgcttc tttccaattc caaataccga
    19561 taactagcga aatcgtaaag acagacaaac tatcccaggg agccggaata taaaaggctc
    19621 cttcttgtat gaagcttgcc attcctacat atcctaaaac aactagaaga actatagtcc
    19681 caacaacaat gtaagtgcca attttcattt taggagaatc ttggactaaa cttcttcgta
    19741 aaattgtggc cacaagtcca aatccaatca gaaaaataag aagttgccct aaaaatgtga
    19801 gcaaattgac tgttaagaga ggacctttag aaaaatcact tagtagttga taataatgta
    19861 ataccgccag gacaagaatt ggcgtcaaaa gggactcttt gatagaactg cgaggtgctc
    19921 ccttgagaat ctctttcatt atttttttag gattcttacc tagataatcc tctgcactca
    19981 tgccatctcg ttctgcttct gagaaatcta gcatcatcaa atagatctgc tctctgagat
    20041 agtcttcatc atagagaaat ccagcaagat taaaactttc ccacaactcc tcaaaatact
    20101 tttgattctc ctcagaaaac tcatgtagca aagcgcttgt ttcttcgtaa tacttcattt
    20161 tcttcatggt ttaaccccca ttcttaatcc cttctacttt ttgactcaaa tcgtcccatt
    20221 gttgccaaaa gactgagaca cgctcttctc cttctttcat taatgaaaaa tacttccgat
    20281 ctggaccatc tggcgacggg cgcatgtcgc ctcttatcca ttgatttttt tctaactttt
    20341 gcaacaaagg ataaatagtt cctggaacga tagtatcaaa tccagcctct cgcaaagtct
    20401 gaaccaactc ataaccatac cgctcttttt gaccaatcat atccaagaca caaccttcaa
    20461 gaacaccttt taatagctga gtttctttca tcacttctcc cttctaatct attttgtaat
    20521 acctactagt aacttcacct atagtatatt acttctacac tagtttgtaa agcataatag
    20581 ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc gccctaccgt atgtatggtt
    20641 actgacttcg tcagtttcat ctacaacctc aaaacagtgt tttgagctga cttcgtcagt
    20701 ttcatctaca acctcaaaaa catgttttga gctgacttcg tcagttctat ctacaacctc
    20761 aaagcagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
    20821 gtataaataa aaaaacagaa ctagcctgaa ctagtcctgt ctacttttac ccaatcacac
    20881 ttccatttgg tacagctgga tcaactgtga gaagggttaa tttgccatca tgttcagctg
    20941 agagaatcat accctggctg acatattttt tcatcatttt acgtggtttg aggttagcaa
    21001 cgatttgaac tttcttgccg accaattctt gttcatttgg atagtatttt gcaattcctg
    21061 aaagaatctg acgatcttct ccatcaccag catccaagcg gaattgaagc aacttatctg
    21121 aaccttctac tttagacact tctttgactt ctgcgacacg gatttcaacc ttgtcaaagt
    21181 cttcaaactt gatttcatcc ttgtttagtt tgagctcaac ttcgtccgga ttccattctt
    21241 tttcgactgc tggtttattg ccttccattt gttccttgat ataggcgatt tcttcttcca
    21301 tatttagacg tggaaagata ggtgttcctt tggcaactac agtcacatct gctgggaagt
    21361 cagccaaact caagttttca agactagaaa cttcttccaa accaagttga gtcaaaactg
    21421 cacgactagt ttccatcata aatggttcaa tcaagtgagc aactacacga atgctggctg
    21481 ccaagtggct catgacactt gccaattggt cacgaagagc ttcatccttg gccaagaccc
    21541 atggtgcggt ctcatcgatg tatttattgg tacgagagat cagagtccag actgcttcaa
    21601 gcgcacgtgg atagtcaact gcttccatgt gtgtatggaa gtctgcgatt gattgttctg
    21661 caacctcagc aagaacatga tcaaattcag tcacaccttc tacataggca gggatttgtc
    21721 catcaaagta cttattaatc atggaaaccg tacggttaag gaggttccca aggtcattag
    21781 ccaattcata gttgatacgg ccgacatagt cttcaggagt aaaggttccg tctgaaccaa
    21841 ctggaaggtt acgcatgagg tagtaacgaa gtggatctag tccataacgc tctaccaaca
    21901 tttcagggta aacgacattc ccttttgact tagacatttt tccgtctttc atgacaaacc
    21961 aaccatgggc aatcaaacga tcaggtaatt taacatccaa catcataaga aggattggcc
    22021 agtagataga gtggaagcga aggatatctt ttcctaccat atggaagact gttccattcc
    22081 agaacttgtc aaagttacca tgttcgtctt gagcgtagcc aagagctgtc gcatagttaa
    22141 gaagggcatc aatccaaacg tagacaacgt gttttggatt tgatgggaca ggcactcccc
    22201 atgtaaaggt tgtacgagat accgccaaat cttccaagcc tggctcgatg aagttgcgta
    22261 gcatttcatt aaggcgacca tctggcgtga taaattcagg atgagctttg aaaaattcga
    22321 ccaaacggtc ttggtatttg ctaaggcgaa ggaagtatga ttcttcagaa acccattcaa
    22381 cctcatgacc tgatggagca ataccaccag tcacatttcc agcttcatca cggaaaactt
    22441 ctgccagctg gctttctgta aagaattctt cgtctgatac tgaataccaa ccagagtatt
    22501 cacccaagta gatatcatct tgagcaagta agcgttcaaa gacttgtgcg acaacttttt
    22561 catggtagtc atcagttgta cggataaatt tatcgtatga gatatctagt aattgccaga
    22621 gttctttaac tccaaccgcc attccatcaa cataggcttg aggtgtaata ccagcttctt
    22681 ccgctttctg ctggattttc tgaccatgtt catcaagacc tgtcagataa aatacatcgt
    22741 agcccatcag gcgtttgtaa cgtgctagga catcacatgc gatagttgtg taggcagaac
    22801 cgatatgaag tttcccagat ggatagtaaa tcggcgttgt aatataaaaa tttttttcag
    22861 acataatttt tcctttccag gcaaatgaaa cctgtttttc taacacttca ttatatcaca
    22921 tttttaatga atttcgatag ggaaatccat acaaaaacaa gatagacgag tgtccatctt
    22981 gttgatctca ttcataacga agggcttcaa ttggatcaag tttcgatgcc ttgttggctg
    23041 gcaagactcc aaaaatcata ccaacactag ccgaaactgc aagactaaat agggcaactg
    23101 ggattgatac tccaacttct ataccttcta ttaaaccttg cagtaacaaa cctgctaagg
    23161 cagttaaacc acttgcaatt gtcaagccaa ttaagccacc taacaaggtc aaaatcatgg
    23221 attcaatcaa aaactgaatt aaaatattgg cacgtgttgc acccaaagcc ttacgaagac
    23281 caatctcacg agtgcgctct gtcaccgaaa ccagcatgat gttcatgaca ccagttcctc
    23341 caacaaagag agaaatccct gcgatggaac taataatcgt cgtcataaaa ctaaacgatt
    23401 gttgaatttc tgcaaataca acggactcat ctgccacctg gtattctccc tgttgtaagc
    23461 ctgcaagctc tgtcattttt cgtgccagtt ctggacccag agttggggtt aaactggtat
    23521 cattcactcg aaagacaata ttagctattt catctacatt aaaattcgca gcaagggaga
    23581 tattggtagt aataggcaag ccaccaaacc catatatttt tgatctttta gcctccggac
    23641 tagtataaac cccaatgacc cggtaactaa atccattgac ttctacaacc ttgttaatag
    23701 cctcttgagg agattcaaat aaactaatgg acaattcctc atctagcaaa atgacacttg
    23761 caaactcttt gaaatcttgc tctctcagac tacgacctgc aataatttca ttcttaacag
    23821 cgtccatgta agttctgttt ccacctgtca aattagcatt ctcaaccttt ttatcttgat
    23881 aggtcaagat ggcattcgtt gaattggtta catagtaact atccactccc ttcagtttag
    23941 ctgcctcttg aacccaggat tcttgcggtt ttggcggttc aacaggaact tcctcttcct
    24001 ttccagaaac cgtaaaagct gattgtttct gagtaaaaga cccatcttta ctttttttag
    24061 gagagaaaaa gacgctaata tttttctgaa atttagtcat atctttattg acttgacgag
    24121 atagggaatc acccaaagcc ataatcacaa caactgatga aacaccgata ataatcccaa
    24181 tcatagtaag caaagaacgc atcttgtgag ccatgataga tgaaaaggca aatttcagat
    24241 tctgcatctt agttttcctc ctttcctaac tgagcactgt cagacgaaat gaccccatcc
    24301 cgaatgacaa tctgacgttt ggcataggca gcaatctcag gctcatgcgt taccatgata
    24361 atggtttttc cttctttatt caaatcaacc aataattgca taatttggtt acctgttttg
    24421 gtatccaagg ctcctgtcgg ttcatccgct aggataatag aaggattgtt taccaaggca
    24481 cgcgcaatgg ctacacgttg cttttgacca ccagataatt ctgaaggtaa atggtgacta
    24541 cgttctgtca attcaacctt gtctaaatat tcctcagcca acttgcgacg ttttgaagac
    24601 gaaactcctg cgtaaatcaa gggcaattct acattttgca gagcattgag cttcgataga
    24661 agaaagaact gctgaaagac aaaaccgatt tgttggttac ggaccttagc cagttgtttt
    24721 tcaccaagcc cagccacttc ttgaccttca agataatatt ctccactggt tggtgtatcc
    24781 aacatgccaa tcgtattcat cagagtggac ttaccagacc cagatggtcc catgatggct
    24841 acaaattcac cctcattcac ttctagattg atatttttga gaacctgcag ttcttggtca
    24901 ccattacggt agcttctgaa gatatttttt agactaatta gttgcttcat cagccttcac
    24961 ctcttttcct tcttccaagg aagatgttgg attactgatg accttagcac catttgtcaa
    25021 accagaagtg atttcttgat tttctgcgtc agcatttccc aatgaaacct caactttttt
    25081 agccttttgt tgttcatcca caatccagac ataattttta ctatcatcca ttactagact
    25141 gctaacagga acaagaatag ccttagtttt gcttttaacc tcaatgttga cagaaaaacc
    25201 ttgtttcaaa tcaccaacct cgcctgtcac atcaatagta taagggtatt tagaacctgt
    25261 attattcccg gctgctggac tagctgcttc accattgttt ttaggatagt cagaaatata
    25321 gcttaatttc ccagtccatt ttttatcagg atacacttta gaagtaaagc ttacttcttg
    25381 acctacagaa aggttggcta gattgtactc agacaattct cccttgactt gtaaattttc
    25441 attgctgaca atatgaacca taacttgact cgcccctgtt ggagatttag aaacattgct
    25501 attgacttcg accacagttc cctctagggt actgagaaca gttgttgcat ccaattgact
    25561 ttgagccttg cttaattgcg ccgcagcatc tgcacgcgca tcacgggcat cacccaattg
    25621 agcgtcaata gaagcaacag aatttccagc cactggagtt gggctttgca ccgttgcatc
    25681 ttctcctcct actggcgctg gtaactgtgg agccggagct gaagcggctt catttcgtgc
    25741 ttgattgagt tcattgatat gacgatctgc cctagctact gctcgactag ctgaatcata
    25801 ggccgcctgc gcttctgaac tactgtactt gactaaagcc tgcccttcgc tgaccttatc
    25861 gcccacagaa acaaggattt catctaaatc acccttacta gcatcaaaat aaacatattg
    25921 ttcatttttt gctgttactg tccctgacaa taaaacagag gaggccacgc ttccttcctt
    25981 ggcaacaaca agatgagtag gctcatcttt tagagcagtc tgagaaggtt gtctaaagag
    26041 taaaatcccc ccagcaccca atacaactac actcgcagca ccgattgctg catacagttg
    26101 ccacttttta gctttaccat tctttttctt cataatgaaa ctccttttct tttttacaat
    26161 actttgctat tataccaaat ttccctccag caaacaatac agttcaggat taaacaatcg
    26221 ttcggaattt tgcttttcta ttgtactaga tatataatac actatcttga cttatttggc
    26281 aagctttttt gcaatttttt gtgcgtagca gatttttgca atcaaatcaa aaaaagaata
    26341 aaatataact atcaaaaaag acgctctact atttaaaaga ttacaaagga gttttcaatg
    26401 agagaatatg atatcattgc tatcggtgga ggtagcggag gaatcgctac catgaaccgt
    26461 gctggtgaac atggagccaa agcagccgtt attgaggaaa agaaattagg tggaacctgt
    26521 gtcaacgtcg gttgtgttcc taaaaaaatc atgtggtacg gggcacaaat cgctgagact
    26581 ttccatcaat ttggagaaga ctacggcttt aagactactg atcttaactt tgactttgca
    26641 accctacgtc gcaatcgtga atcctacatt gatcgcgctc gttcttctta tgatggcagt
    26701 tttaaacgca acggtgtaga cttgattgaa ggtcatgctg aatttgtaga ttctcatact
    26761 gtaagcgtaa atggtgaact gattcgtgct aaacatatcg tgattgctac aggtgcccat
    26821 ccaagtattc ctaatattcc tggtgctgag ctaggtggct cttctgatga tgtatttgcc
    26881 tgggaagaac ttccagagtc agttgccatt ctaggcgctg gttatatcgc cgttgaatta
    26941 gctggcgtac tccacacttt tggtgtcaag acagatctct ttgttcgccg cgatcgtcct
    27001 ttacgtggtt ttgattccta catcgttgaa ggtttggtca aggaaatgga aagaacaaac
    27061 ttaccacttc acactcacaa agtccctgtc aagttagaaa aaactactga cggcattacc
    27121 attcatttcg aagatggtac tagtcacaca gctagccaag ttatctgggc tacaggtcgc
    27181 cgtccaaacg ttaagggctt gcaacttgaa aaagctggag tgactctgaa cgaacgtggc
    27241 tttatccaag tggatgaata ccaaaatact gttgttgagg gaatctatgc tctaggtgat
    27301 gtaacgggcg agaaagaact gactccagtt gcaatcaagg ccggacgtac cctatctgaa
    27361 cgtctcttta acggaaaaac tactgcaaaa atggattact caactattcc aactgttgtc
    27421 ttttcacacc ctgctatcgg aactgttggt ttgacagaag agcaagctat taaagaatac
    27481 ggtcaagacc aaatcaaggt ttataaatca agctttgcat ctatgtactc tgcttgcact
    27541 cgcaaccgtc aagaatcccg tttcaaactc ataacagctg gttcagaaga aaaagttgtc
    27601 ggacttcatg gaattggcta cggcgttgat gaaatgattc agggatttgc cgttgctatc
    27661 aaaatgggag caaccaaggc tgactttgat gcaactgtgg caattcaccc aactgcatct
    27721 gaagaatttg taaccatgcg ttaatcgaaa caaaagaagc tgatataaat atgtctgctt
    27781 cttttttaat gattttcgaa tcaacaaaaa aattatccaa cttgactagt cacgaaaatc
    27841 ggctgttaca gtttttgtta ccttgttttt aaaaaatggt tgacatcgac tcctatatga
    27901 gtataatagt tactatacat tagaaaagag gttaactatt ttgaaaaaag ctcacgttta
    27961 tgctatccct gctattgggg ctgctctcat tgctgttttg gcacaaatca gtcttccaat
    28021 tggacctgtt cccttcactc tgcaaaactt tgcaatcggc ttgattgcta ctgtctttag
    28081 accgagagag gctgtacttt ctgctggact ctatcttctt ctaggtgcta tcggtcttcc
    28141 tgtctttgca ggaggtggag ctggttttca ggctttagtt ggccctactg caggctatct
    28201 ttggttttat ctcgtttact ctggacttac ttcctctcta accaacagca agagtggtgt
    28261 tgttaagatt tttcttgcaa acctcttggg tgatgccctt gtctttgtcg gcgggattct
    28321 cagcttgcat ttcctagctg gaatggcatt tgaaaaagct cttgttgtgg gggttcttcc
    28381 ctttatcatt ccagaccttg gcaaacttct ggctattagt tttattagcc gtcccctact
    28441 tcaacgcctt aaaaatcagg cttactttac taactaaaaa aggataccga gttatcatga
    28501 ctcaatatcc ttttttttat tttgaaaact tatactcaat gaaaatcaaa gagcaaacta
    28561 ggaagctagc cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa actgacgaag
    28621 tcagtaacca tacatacggc aaggcaaagc tgatgcggtt tgaagagatt ttcgaagagt
    28681 attagttgcc tttaaaggca tccaccatcg tttgaaattc ttcatttgag agagtaatcc
    28741 ctttgcccat tttagtatgg tttggactcc aagcacgaat atcaaacttt gcaggggcac
    28801 cattaaagct cacacggtta atttccttgg tccaaccttt ttcgttttca gaaagagtca
    28861 acaagtgctc ttcgatttca aatgtaaatt ctgccatttt cttctccttt tttagtttca
    28921 ttagtttatt cgtaaaatct tgtagatttt aggaaaattt tatataatat tgatataaaa
    28981 taagggaggc caatatgaga cataaattcc agcaagttct agataaaata catgattttt
    29041 taaatggaca tgaccaacct gaccagactg aaaccaactc ccttacagcc actattgaag
    29101 aggctatcca gaaacaaacc gctgttcacc ttatcttgtc tgagacaagt tttacaggtg
    29161 atatcatcaa atatgatcag caaggccagc aaattatcgt gaaaaatttt gccaaaaatg
    29221 tgagccggat tatccgtata agcgatattc aacgcctgcg atttgtcccc tcaactgtcc
    29281 aaacagccca aaaaaataga tttaagaaag agtgagatgt agttgcttca tcccactctt
    29341 ttttcttagc gaatttgttc aaaatgtaaa tgaactgcaa tatgatctcc ataaccactt
    29401 ctttccaagt cacgttgtaa acgataggaa atgtagtgtt ctgcaatagt aatgtaacct
    29461 gcgcccaata aacgatgttc aaccatagat tgaatcatac tgatagtcgc acgttccacc
    29521 ttggcttctt gtaaatccaa aactaccttc ttagtgactt gagcaagatt ttgacgcaaa
    29581 tcatctgtca aaacataaac agtttgggct gccttcaaga tggcttggta aatcttatct
    29641 ggattaaatt cagcaatttc gccattacgt ttgattactt gcataggttt ctcctttatt
    29701 ctttgttttc tttgatttct gccagcattt tttcttcttc tactgtcagt tgataatgtt
    29761 caagtaaatc cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat cgccactgac
    29821 gaatcttttc atggtggcca ctcatcaata catctggcac gaccatgcct cgataatcat
    29881 agggacgtgt gtactgagga tattctaaaa gacctgaaga aaaactatca tcttggtggc
    29941 tagactcctt gccaatcact tctggaatca ggcgaactgt agcatcaatc atggtcatag
    30001 ctgccaattc tccaccagtg aggacatagt cgcctaggga aatctcatct gttaccaagg
    30061 tcttaatgcg ctcatcataa ccctcatagt gcccacagat aaagattagc tcttcctctt
    30121 gagccaaatc ttcagcataa gcctgatcaa actgctttcc agcaggatcg aggagaataa
    30181 cgcgcggatt tttcttttca atagcatcaa aggaatcgaa aataggttgt gctctgagca
    30241 acatgccctg accgcctccg tagggctcat catctacatg acgggccttt tcagcatttt
    30301 ctcgaaaatt atgatactgg atatccaaga gccctttttc tcgagccttt ccaacgattg
    30361 agtgctccag tggagaaaac atctctggaa agagggttaa aatatcaatc ttcatcgtct
    30421 aacccttcta agatttccac atcgacccgt ttatttggaa tatcaacatt gagaaccact
    30481 ggtgggatat aaggtaaaag caaatcacgt ttgccttttc gtttgaccac ccagacatca
    30541 ttagcacctg gttgcaggat ttccttgatg gttccaacca agctatcacc ctcatagact
    30601 tccaaaccga taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt caaatcttcc
    30661 tcagcgacct tgagactgta tcccttgtac ttttcgatag tattgatatg gtacatatct
    30721 ttgaatttaa taatgtcaaa gttcttctgt ttacggtggc tagcgatggt cactgtttgg
    30781 acaaactgat ctttttcatc aaacaaagcc agctcagctc cttttttaaa ccgttcttct
    30841 gcaaaatccg tcacagacaa gactcgcatc tcaccctgta atccctgcgt attaacgatt
    30901 ttcccaacat taaagtagtt catcttgtct cctgtaatct ccttttttcc atcttattct
    30961 gacaattctc gaataatagc cgcaattttt tccgattctg accattgtaa ataatggtga
    31021 ttccctccta aaatgagttt agtattggaa gtccaatatt ctgattctct gtactctttt
    31081 tctctataag gctgacaaaa aacaaataca ggaatatgag cttctataga tacatcctca
    31141 aaatcttcct cagtaatctc tccagatatc tgaaattctg gatcttgatt ttccaactct
    31201 aagccttttt cttgcattaa ttcccagatt tttttattcg tttcaggact aaatgttgct
    31261 tgagttaaat tcttcaaata aagttcaggt ccatactcgt caatcagcct catctgctct
    31321 tccatttctg gataaggatt ttctgaaaaa tcagcaaaca tgactttttt agttgtcggt
    31381 tcaattgcta ctaaagtctg acacttaatt ggtttctcga gtaatttgca agctaaaatt
    31441 ccactccaac tatgtgcaca aagtatatat tcagaaatcc ctaattcttc aagtacttca
    31501 taaaccacat ctgcaagatt atctagattt tttccagctt ggtcatgaat cggactccta
    31561 cctgtgttcg gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt ttcaagtata
    31621 agtgaaaaat tttcataact tggtagcaaa cctgctccgt ttagacaaac tagcactttc
    31681 ttttgctttt gataagtaac agagaggcta ccaatttctg tagatacttc aaacctcttc
    31741 ataaagaaat ccactcattc tatataatga attattaaaa atccttatcc tttattttat
    31801 cacgttccaa ggattttctc aagttggagg aaggggacaa catctctact ttcccttcaa
    31861 gaatccttcc aaatcatgtt tatgttggta attaacgact gcggttttgt ctttctcaaa
    31921 gacagtcttg gtaaggtcaa tatgcttgat agcagcaata gcgacggtgt agtaaatgat
    31981 atcagccagt tctctggcaa gttcctcgtt cgaatcctat cccttctttt cgaccagagc
    32041 gcctattcaa aacctcggct acttctccga cttcctccac taacttcata aagagacctt
    32101 catcagtccg agactgctgt taatgttcga gtaagtagtc ttggaattgc ctaaacgttc
    32161 aatcttttat agtatattga aactagaata gtacaccttt acttctaaaa cattgttaga
    32221 aatcgatttg actgtcctga tcgatttgtc ctgttcttgt ttcattttac tatatcttct
    32281 attccacaca aaaaagcgag acatccgtcc cgcccttctt atttttcgtc aataacgatt
    32341 cttacttttt tgtattcagt tgggacagag tagacaatcg ttcttatcgc agaaatagtg
    32401 cgacccttac gaccgattac acgacccaca tcgctttgat caagattcaa atgatattcc
    32461 aaaaattctg gtgtatcctc aatcttgata gttaaggcat ctggttgtga aattaagggt
    32521 ttcacaatcg caataatgag attttcaatc gtatccatct gtcaacctac tttaaactta
    32581 ttttgaaaat ttagaatcgt ggaatttttt caatacgcct tcttttgaaa ggatgttacg
    32641 tactgtgtct gaaggttgag ctccattagc caaccatgca agaacgcggt cttctttcaa
    32701 agttacttgg ttttcagcaa caagtgggtt gtaagttcca actgtttcga tgaaacgtcc
    32761 gtcacgtggt gaacgtgaat ctgctacgtt gatacggtag aaaggttttt tcttagaacc
    32821 catacgagtc aaacggattt taactgccat ttttaaagtc tcatttcttt aattttttat
    32881 ttcggtgaaa tagctgagct atttagcaca tgttctatta tagcagattt ctggcatgtg
    32941 tcaagaaaa
//
LOCUS       DAVLTM010000009        31044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLTM010000009 DAVLTM010000000
VERSION     DAVLTM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.009"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..228
                     /locus_tag="VYN16_000368"
     CDS             <1..228
                     /locus_tag="VYN16_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1402955.1"
                     /translation="YPQAQTPRKSSKLKPLTVEDKAYNHALSKERSKVENIFAKVKTF
                     KMFSTTYRNHRKRFGLRMNLVAGIINHELGF"
     gene            complement(225..470)
                     /locus_tag="VYN16_000369"
     CDS             complement(225..470)
                     /locus_tag="VYN16_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1402956.1"
                     /translation="MEQSPKKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(485..1657)
                     /locus_tag="VYN16_000370"
     CDS             complement(485..1657)
                     /locus_tag="VYN16_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1402957.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(1716..2561)
                     /locus_tag="VYN16_000371"
     CDS             complement(1716..2561)
                     /locus_tag="VYN16_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1402958.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            2686..3243
                     /locus_tag="VYN16_000372"
     CDS             2686..3243
                     /locus_tag="VYN16_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1402959.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3262..3729
                     /locus_tag="VYN16_000373"
     CDS             3262..3729
                     /locus_tag="VYN16_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1402960.1"
                     /translation="MTEKRLTWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            3818..4447
                     /gene="upp"
                     /locus_tag="VYN16_000374"
     CDS             3818..4447
                     /gene="upp"
                     /locus_tag="VYN16_000374"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1402961.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4621..5211
                     /gene="clpP"
                     /locus_tag="VYN16_000375"
     CDS             4621..5211
                     /gene="clpP"
                     /locus_tag="VYN16_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1402962.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5290..5538
                     /locus_tag="VYN16_000376"
     CDS             5290..5538
                     /locus_tag="VYN16_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1402963.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5640..6800
                     /locus_tag="VYN16_000377"
     CDS             5640..6800
                     /locus_tag="VYN16_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1402964.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            7068..7937
                     /locus_tag="VYN16_000378"
     CDS             7068..7937
                     /locus_tag="VYN16_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1402965.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            7941..8897
                     /locus_tag="VYN16_000379"
     CDS             7941..8897
                     /locus_tag="VYN16_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1402966.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            8897..9661
                     /locus_tag="VYN16_000380"
     CDS             8897..9661
                     /locus_tag="VYN16_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402967.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            9661..10371
                     /locus_tag="VYN16_000381"
     CDS             9661..10371
                     /locus_tag="VYN16_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1402968.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            10678..11334
                     /locus_tag="VYN16_000382"
     CDS             10678..11334
                     /locus_tag="VYN16_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1402969.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            11442..12537
                     /gene="prfB"
                     /locus_tag="VYN16_000383"
     CDS             join(11442..11513,11515..12537)
                     /gene="prfB"
                     /locus_tag="VYN16_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1402970.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            12555..13247
                     /gene="ftsE"
                     /locus_tag="VYN16_000384"
     CDS             12555..13247
                     /gene="ftsE"
                     /locus_tag="VYN16_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1402971.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            13240..14166
                     /locus_tag="VYN16_000385"
     CDS             13240..14166
                     /locus_tag="VYN16_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1402972.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLRI"
     gene            14452..16632
                     /locus_tag="VYN16_000386"
     CDS             14452..16632
                     /locus_tag="VYN16_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1402973.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            16684..17499
                     /locus_tag="VYN16_000387"
     CDS             16684..17499
                     /locus_tag="VYN16_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1402974.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAVLNWK"
     gene            17705..19300
                     /locus_tag="VYN16_000388"
     CDS             17705..19300
                     /locus_tag="VYN16_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465985.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1402975.1"
                     /translation="MFFQDRKIKYSIRKLSVATVSLAIGFLFAPAALGVAQVAQADEL
                     STTEQVENKAENQVSVVSFATEQEESETTYVDTTNKVTWTFAGYDKEKIVVGKGRQTF
                     LGSWVPTPNPEYVFKSSKAGVPLPQSILGMLPKDEASYKVGDTIVAKQPAVESVVEEE
                     KDYVWTFKGYDQKNATYNGKRVTFTGIWEVTPRPHHVSYTFVSVTSENGNSVSAKQPA
                     QATYIDSVNDGTWTFKGYDAASAVVNKANVEFVGKWEFKANPTNAETYTPQVTEETIK
                     VGQTPDLTDNVTNLPNLPAGTKVVDITPAGQIDTTKPGTYTGKVRVDYPDGSSTEVSV
                     SVNVLPAPETQTYKVTYRFESATADNNLPAEVLSLLPQSGTYTTSSSVAIAFVPEAPM
                     PVEVAVANGTWTFLGYQEVEEGSNLTFVGKWVFEAKQDPSPQPQPAPTPQPAPQPNPV
                     PPVNPGGEQGTDNNQADRHQSEQPRKPEENPPAQGPAGEKEKQATLPNAGSTAPLSLT
                     GLVTSSLLAGLAVLLLGRKRKDD"
     gene            19680..21023
                     /locus_tag="VYN16_000389"
     CDS             19680..21023
                     /locus_tag="VYN16_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1402976.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            21238..22428
                     /locus_tag="VYN16_000390"
     CDS             21238..22428
                     /locus_tag="VYN16_000390"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1402977.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            22981..23916
                     /locus_tag="VYN16_000391"
     CDS             22981..23916
                     /locus_tag="VYN16_000391"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1402978.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            23950..24987
                     /locus_tag="VYN16_000392"
     CDS             23950..24987
                     /locus_tag="VYN16_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1402979.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            25160..25765
                     /locus_tag="VYN16_000393"
     CDS             25160..25765
                     /locus_tag="VYN16_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1402980.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            25922..26452
                     /locus_tag="VYN16_000394"
     CDS             25922..26452
                     /locus_tag="VYN16_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1402981.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            26473..27558
                     /gene="rlmN"
                     /locus_tag="VYN16_000395"
     CDS             26473..27558
                     /gene="rlmN"
                     /locus_tag="VYN16_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1402982.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHIMVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            27558..28040
                     /locus_tag="VYN16_000396"
     CDS             27558..28040
                     /locus_tag="VYN16_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1402983.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            28042..29583
                     /locus_tag="VYN16_000397"
     CDS             28042..29583
                     /locus_tag="VYN16_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1402984.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(29639..30442)
                     /locus_tag="VYN16_000398"
     CDS             complement(29639..30442)
                     /locus_tag="VYN16_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1402985.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(30525..30737)
                     /locus_tag="VYN16_000399"
     CDS             complement(30525..30737)
                     /locus_tag="VYN16_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1402986.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
BASE COUNT         9389 a         5757 c         6760 g         9138 t
ORIGIN      
        1 tatcctcaag cacaaactcc acgtaaatcc agcaaactca agccgctaac agttgaagat
       61 aaagcctata accatgcgct atccaaggag agaagcaagg ttgagaacat ctttgccaaa
      121 gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
      181 cgaatgaatt tggttgctgg cattatcaat catgaactag gattttagtt ttgcaggaag
      241 tctaatagag tatgaacctg ttctcgtatt tcttcgagag ctaagttact ttcaagaata
      301 atatttccac tttgaatata agttcgaact tgttgaaaat cagcttctgt caagatatct
      361 actagataag acattttagg gatagcattt tttccattag gagtgactcc ccttagcaag
      421 ataatatgtt ccaaagtagt tcctttcttt tttttggggg attgttccat ttagtaatgt
      481 atatttagat tggatttgat cccgccaccc agccggtaca gagagcagaa gtgatgttaa
      541 agccacccgt gtgggcattg atatccataa cttcgcctgc aaagtggagg ccaggtacca
      601 gcttactttc aagggtttta ggattgattt ccttgagact gactccaccc ttggtaacaa
      661 aggactttgc aagggacatt tttccagtta caggaatttt aagttcttta atggactgga
      721 caagttgttc tcgttccttt tcagtcagct gtttgacttt ttcaggatat ccttgtacaa
      781 aaaattcggc caagcgttct ggtaacaagg tttttaaagc gtttttcaag gatttttccc
      841 gattttcttc tagaaatgta accaagtcct tctcagaaag ttgaggcaaa acatcgagtg
      901 agagaacctc cccacctttg acaaagctag acatgcgtag ggcagcagga cctgacaaac
      961 caaagtgggt aaagagtaaa tcatgagtga tgacatgctt accataactt agggtcacat
     1021 cgtccaaaga aatcccttgt aaggctttat gtggaaaatc tgttaataaa ggactttcag
     1081 cagcctcaag ctcggtgatg gtatgcttaa aatggcgagc aatctcgtga ccaaaaccag
     1141 tcgaaccagt cgaaggataa gacttaccac ctgttgtgac aatgagtttc tcacaagtga
     1201 aggtttgatc cgctgactta aggacaaact ggtcatctac ttttttaaca gaaacgattt
     1261 ctatttgagt agcaacttga ccacctagtt cagtgatttt cttttccaaa gcttcgataa
     1321 tagtccgaga cttgtcactg gctggaaaga cgcgtccgtg gtcttcgacc ttaagtttaa
     1381 caccattttc tgtaaaaaag ttgatgatgt catgattatc gaactgggag aaaacactgt
     1441 aaagaaagcg tccgtttcca ggaattccag ctagcaggtt gtctaagcta ccattgttgg
     1501 tcacattgca acgtccccca ccagtcccag ctaatttttt tccaagtttc cgattttttt
     1561 cgatgaggag ggttttctgt ccataaaagc tactggaaat cgtagccatc ataccagcag
     1621 gtcccccacc gatgacaata gtatcaaaat gtttcatagc tctattgtac cacaaaaaaa
     1681 caagagatga tggtcacctc ttgtcaagaa tgcaattaat caatttcata gcccatcagc
     1741 aaaccgccct cttctgcata gaaactgcag agaccagagg ttggtagaat tttaatatcc
     1801 gcttgtggga aggtttcacg gattcgctct gagagctgtt gacaacattt ttcgttattg
     1861 cgttgggcca tgacaatacg gccaccagca tatccagctt ttactaactc atcataggca
     1921 gcttgaactg atttctttga tccccttgct ttttgtagca attcgagagt tccagtttca
     1981 ctagcttttc cgaccatacg aatgttgaga aggccaacga ccgtaccgat aagcttgctc
     2041 aaacggccgt tcttcaccaa gttatcgact ttggctagga caaagagcaa cttagttttt
     2101 tcttgatagg cggtgatagc ttcaaccact tcttcaaaag acaagccctg gtcaatcaag
     2161 tcattcaatt tttctacgag taggtcaact tcaccaccag cagataaact atcaatcaca
     2221 tgaatcttag tgtcaggatg gtcttccaga taaatattct ttgctagttg agcactattg
     2281 tgactgccag aaagggtacc tgtgatggtt actaggaaaa tgtttttggc accttcaaat
     2341 gctcgcaaat agtcatctgg gcttggacaa gccgattttg aagcttctgc agttgcatac
     2401 atggtttcca tcatttggtc aatatcgaga ctggcgtcat caacaaagac ctgatcagct
     2461 acttgaatgg ttaaggggac acttacaaag gttgtgttaa tagctggtgt tggcagttga
     2521 cgataatcac aaccagagtc agcaataatc ttccaagtca tagaaattct ccatctttgt
     2581 cagttataca ttgacaaagg ttctgtcttt ttttacaatt atatcatgaa aacccttgaa
     2641 acaaaagcat catccgtctg ttgtgacaag tagaaagaaa atgttatgtc tgaacgtaga
     2701 atctctgaaa agtctcttga aaatctcaga aaatcaaacc aagaatccaa tttattaacc
     2761 agagaagcca ttgaaacagc cctcttgcaa ctcttggaaa aaaaggaact gaccaagatt
     2821 agtatttctg aattggtcaa acgtgcaggt gtttcgcgtg cggcctttta tcgcaattat
     2881 gactccaaag aggaaatttt agagagcgtc tttaaacgaa ctgtccacaa tattatggaa
     2941 cagctgcatc attacgattt aaagacagac ctttatttgg tttgggttca ccttttccgg
     3001 gaggccagaa aggaagctag agtgattcaa ttggccttgg attaccatct ggaaaaaatc
     3061 tttgtccaag ccatgcagga atttctagaa aaataccatg ggaaatcaaa aggtgtcagc
     3121 tcttatcttc attccttctg gagctcagcc atcgtctctg tccttctaaa atggatcaag
     3181 gatggcatga aggtaccagc tgaaaagatt gcagatttag gtttaccatt ttttaaaaaa
     3241 tagagaaaaa ggagaaaaga gatgactgaa aaaagactga cctgggatga gtattttgca
     3301 gcccaagccc tactaattgc gaatcgttcc acttgtaaac gtgctaaagt gggcgcgatt
     3361 ctggtaaaag ataataaggt tatttccact ggttacaatg gttcggtgtc agggactgag
     3421 cattgtattg atcacgaatg tttggtcatt gaaggccact gtgttcgcac ccttcacgct
     3481 gaggtcaatg ctatccttca aggtgctgag cgtggtgttc ccaaaggctt tacagcctat
     3541 gtaacccatt ttccttgtct caactgtaca aaacaattgc ttcaggtcgg ctgcaagcgt
     3601 gtggtttata tcaaccagta ccgaatggac gactacgccc aataccttta tcaagaaaaa
     3661 gggacagaat tgacccattt accacttgag acagtacaga cagctcttaa agaggctgat
     3721 ctaatgtaaa aattatcaaa aataaatggt ttagaaagat ttttaaaccg ttttttggta
     3781 taataagaag aataaattga aagaaggaat tccaaaaatg ggaaaaattg aagttattaa
     3841 tcacccactg attcaacaca aattgtcaat cttgcgtcgt acagatactt ctacaaaagc
     3901 ttttcgtgag ctagtagatg agattgcaat gttgatgggg tatgaagtac ttcgtgatct
     3961 tccactagaa gatgtggaaa tcgaaacacc aattacaaaa acagttcaaa aacaattggc
     4021 aggtaagaaa ttggccatcg tcccaatctt gcgtgcaggt atcgggatgg ttgatggtct
     4081 cttgagcttg gttccagctg ctaaagttgg ccacatcggt atgtaccgtg atgaagaaac
     4141 acttcaacca gttgaatact tggtgaaatt gcctgaggac attgaccaac gtcaaatttt
     4201 tgtagtagac ccaatgttgg caacaggtgg ctcagcaatc ttggctgttg attctcttaa
     4261 aaaacgtggc gcatcaaata tcaaatttgt ctgccttgta tctgctccag agggtgtaaa
     4321 agcccttcaa gaagctcatc cagatgtaga aatctttaca gcagccttgg atgaacgttt
     4381 gaacgaacac ggttatatcg ttccaggtct tggagatgct ggagaccgct tgttcggtac
     4441 aaaataagat cgaaaagaag aatgacttgg aaaagtattt ccagtcacga aaggaggttg
     4501 ggtttttgtt tctgtctaat gaaagcagag caaaaatttg accttttttg accaagatat
     4561 tataatagtc ttatcttcag tcatttgacc aacaaaatta aaactcaaaa ggagaaatga
     4621 atgattcctg tagttattga acaaacaagc cgtggagaac gttcttacga tatttactca
     4681 cgtcttctca aagaccgcat cattatgctg acaggtccgg ttgaagacaa tatggctaac
     4741 tctgttattg cccaattgct tttcttggat gcccaagata gtacaaaaga tatttacctt
     4801 tatgtcaata caccaggtgg ttctgtttca gctggtttgg caatcgtaga taccatgaac
     4861 tttatcaagg cagatgtcca aaccattgtt atgggaatgg ctgcatctat ggggactgtc
     4921 atcgcatcaa gtggagcaaa aggcaaacgt ttcatgcttc caaatgctga atacatgatt
     4981 caccaaccaa tgggcggtac aggtggtggt acccaacaaa ctgatatggc tatcgctgca
     5041 gaacacttgc tcaaaactcg taataccttg gaaaaaatct tggctgaaaa ttcaggtcag
     5101 tcaatggaaa aagtccatgc agatgcagaa cgtgataact ggatgagcgc ccaggaaaca
     5161 cttgaatatg gctttattga tgaaattatg gccaacaatt cattgaacta atgatgatag
     5221 aaggcaaact cgactgggtt tgcttttttt ggtataatag ggagagattt cttagaaaga
     5281 ggatttatca tgtttgaaaa agtcaatcgc tctggcttga ttatctatct ttactataat
     5341 cgtgatgcca aaaaactgca ggattatgga gatattacct atcattccaa gaaacatcgt
     5401 tacttacaac tctatgttcc aactcaagaa gtggagcaat tggtcggacg cttgagcaag
     5461 gaaaaattta ttaaaaaagt tagggtttgt catatccaag agttggaaac accctttgtg
     5521 ggcaatcttt atcgatagga aaacgttatc atcgaaaaaa gctcaaggaa aacattgaca
     5581 attttctgat aattcggtat attcttaaca gactatttaa gaaataagga gacaaaaaga
     5641 tgaagaaaaa atttgcccta tcgtttgtgg cgcttgcaag tgtagcactt cttgcagcct
     5701 gtggagaagt gaagtctgga gcagtcaaca ctgctggtaa ctcagtagag gaaaagacaa
     5761 ttaaaatcgg gtttaacttt gaagaatcag gttctttagc tgcatacgga acagctgaac
     5821 aaaaaggtgc ccaattggct gttgatgaaa tcaatgccgc aggtggtatc gatggaaaac
     5881 aaatcgaagt agtcgataaa gataataagt ctgaaacagc tgaggctgct tcagttacaa
     5941 ctaaccttgt aacccaatct aaagtatcag cagtcgtagg acctgcgaca tctggtgcga
     6001 ctgcagctgc ggtagcgaac gctacaaaag caggtgttcc attgatctca ccaagtgcga
     6061 ctcaagatgg attgactaaa ggtcaagatt acctctttat tggaactttc caagatagct
     6121 tccaaggaaa aattatctca aactatgttt ctgaaaaatt aaatgctaag aaagttgttc
     6181 tttacactga caatgccagt gactatgcta aagggattgc caaatctttc cgcgagtcat
     6241 acaagggtga aatcgttgca gatgaaactt tcgtagcagg tgacacagac ttccaagcag
     6301 cccttacaaa aatgaaaggg aaagactttg atgctatcgt tgttcctggt tactataatg
     6361 aggctggtaa gattgtaaac caagcgcgtg gcatgggaat tgacaaacca atcgttggtg
     6421 gtgatggatt caacggtgag gagtttgtac aacaagcaac tgctgaaaaa gcatcaaaca
     6481 tctactttat ctcaggcttc tcaactactg tagaagtttc agctaaagct aaagccttcc
     6541 ttgacgctta ccgtgctaag tacaatgaag agccttcaac atttgcagcc ttggcttatg
     6601 attcagttca ccttgtagca aacgcagcaa aaggtgctaa aaattcaggt gaaatcaagg
     6661 ataaccttgc taaaacaaaa gattttgaag gtgtaactgg tcaaacaagc ttcgatgcag
     6721 accacaacac agtcaaaact gcttacatga tgaccatgaa caatggtaaa gttgaagcag
     6781 cagaagttgt aaaaccataa tagaaaaatg ttgaaatagg gaatgagcct ttgactcact
     6841 ccctgtttcg atatttaata ctcttcgaaa atctcttcaa actgcgtcaa cgtcgccttg
     6901 gattatatat gtgactgact tcgtcagtct tatctacaac ctcaaagcag tgctttgagc
     6961 aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtataag aacctatcaa
     7021 aaagtgaggg aaaaccttcg gaattataaa tagaaagagt gaatcttatg ctccaacaac
     7081 tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt agccctagga tataccatgg
     7141 tttacggaat tatcaagctc atcaacttcg cccatggtga tatttatatg atgggagcct
     7201 ttatcggtta tttcttgatc aattctttcc aaatgaattt ctttgtagcg cttattgtag
     7261 ctatgctagc gacagctatt cttggtgtcg tgattgagtt tcttgcttac cgacctttgc
     7321 gccactctac tcgtattgct gttttgatta cggctattgg ggtttctttc ctattggagt
     7381 atggaatggt ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg attcaaacag
     7441 ttcgatatga tttgggacca attagcttaa caaatgtgca gttaatgatt ttggctattt
     7501 ccttgatttt gatgattttg ttacaagtca ttgtccaaaa gactaagatg gggaaagcca
     7561 tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat ggggatcaat gtaaaccgta
     7621 cgattagctt taccttcgct ttgggttctg ctcttgcggg tgcggctggt gttctgattg
     7681 ctctttatta taactctctt gagcctttga tgggggttac tccaggtctt aaatctttcg
     7741 ttgccgcagt acttggtggt atcggaatta ttcctggtgc ggctcttggt ggctttgtga
     7801 ttggtctatt ggaaaccttt gcgacagcct ttgggatgtc agatttccgt gatgccattg
     7861 tttatggaat cttgttgttg atcttgattg tccgcccagc tggtatcctt ggtaagaatg
     7921 tgaaagagaa ggtgtaagcg atgaaggaaa atttaaaagt taatattcta tggttactcc
     7981 ttttgttagc tggctatagc ttgattagtg tactggtttc agtcggagta cttaatctat
     8041 tctatgtaca gattttacaa caaattggaa ttaatattat tttggctgtt ggtctcaact
     8101 taatcgttgg tttttcagga caattttcac ttggtcatgc tggtttcatg gcgattggtg
     8161 cctatgcagc agctattatt ggttctaaat caccaaccta cggtgccttc tttggagcta
     8221 tgcttgtagg agctttgctt tcaggagcag ttgccttact tgtcggcatt ccaaccttgc
     8281 gcttgaaggg ggactatctt gcggtagcaa ctctaggtgt ttctgaaatt atccgtatct
     8341 ttatcatcaa tggtggaagc cttacaaatg gtgcggcagg tattttaggg attcctaact
     8401 ttacaacttg gcaaatggtt tacttctttg tcgtgattac aaccattgca accttgaact
     8461 tcttgcgtag cccaattggt cgttcaaccc tctctgttcg tgaagatgaa atcgctgctg
     8521 agtcagttgg ggttaatacg actaaaatta aaatcatcgc ttttgtcttt ggtgccatta
     8581 ctgcaagtat tgctgggtca cttcaggcag gatttatcgg gtctgttgta ccgaaagatt
     8641 acaccttcat caactcaatc aacgttttga ttattgttgt atttggtgga ctcggttcca
     8701 ttacaggtgc gattgtttcg gctattgttc tgggaatttt gaatatgctt ctccaagatg
     8761 ttgctagtgt gcgtatgatt atttacgctt tggccttggt attggtaatg attttcagac
     8821 caggtggact ccttggaaca tgggaactga gcctatcacg tttctttaaa aaatctaaga
     8881 aggaggaaca aaactaatgg cattacttga agtaaaacag ttaaccaaac attttggtgg
     8941 tctaacagct gttggagatg tgactcttga attgaacgaa ggggaactgg ttggattaat
     9001 cggtccaaac ggagctggga aaaccaccct tttcaacctt ttgaccggtg tttatgaacc
     9061 aagcgaggga acagtaaccc tagatggtca ccttttgaat gggaaatcac cttataagat
     9121 tgcctctttg ggacttggac gtactttcca aaatatccgt ctctttaaag atttaacagt
     9181 tttagataat gttttgattg cttttggaaa ccatcacaaa cagcatgttt ttactagttt
     9241 cttacgctta ccagcttttt acaagagtga aaaagaatta aaggataaag ctttggaatt
     9301 gttgaaaatc tttgatttag atggtgatgc agagactctt gctaaaaatc tttcctacgg
     9361 acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa ttctcttctt
     9421 agatgaacca gcagcaggta tgaacccaca ggaaacagcc gaattgactg agttaattcg
     9481 tcgtatcaaa gatgaattta agattacaat catgttgatt gaacacgata tgaatctggt
     9541 catggaagta acagaacgta tctacgtact tgaatatggc cgtttaattg ctcaaggaac
     9601 tccagacgaa attaagacca ataaacgcgt tatcgaagct tatctaggag gtgaagccta
     9661 atgtctatgt taaaagttga aaatctttct gtgcattacg gtatgatcca agcagtccgt
     9721 gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc caacggtgca
     9781 ggtaagacaa ctattcttcg caccttgtca ggtttggttc gaccaagttc aggaaagatt
     9841 gaatttttag gtcaagaaat ccaaaaaatg ccagctcaga aaatcgtggc aggtggtctt
     9901 tcacaagttc cagaaggacg ccacgtcttt cctggcttga ctgttatgga aaatcttgaa
     9961 atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag caaacttgaa gaaggttttc
    10021 tcacgctttc ctcgtcttga agaacgtaag aaccaagatg cagctactct ttcaggaggg
    10081 gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa caccaaaact tcttctttta
    10141 gatgaaccat caatgggact tgccccaatc tttatccaag agatttttga tatcattcaa
    10201 gatattcaga agcaaggaac aaccgtcctc ttgattgaac aaaatgccaa taaagcactc
    10261 gcaatctctg accgaggata tgtactggaa acagggaaaa tcgtcctatc aggaacagga
    10321 aaagaactcg cctcatcaga agaagtcaga aaagcatatc taggtggcta aaacaatcca
    10381 gtggattgtt ttagtcggca gatgaagatt acgaagtaat catcaatata gtccggggga
    10441 cctttttagt cggtagattg agattgcaaa caaatctgca tctacattga aagctgaatt
    10501 tctaataatt gaaaaaatcg aatgaaaagt ttcttacctt cattcacaga actcgatttc
    10561 agagctcttt tgctagctta ttcatacttt tctgaatttc gaaaaagaaa tgtaagcgtt
    10621 tgatagattt gcaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg
    10681 gcagttaaag attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct
    10741 catgcagcag atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat
    10801 caattagttg gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca
    10861 agtctttcta tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg
    10921 attcgcgatg ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg
    10981 ttgaaaaata agattagtat tctccctgtc gtagataacc atcaagtata cggagttatt
    11041 actgaccgtg acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt
    11101 cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt
    11161 gtagaagaaa atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg
    11221 attatcgaag tgcaaatcga tggatcaatt gatttaccag ccttgaaaga aaaatttgaa
    11281 gcaaatggta ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg
    11341 aagcccaaag gcttcttttt tcatgaaaag gggattagag aaaaagatgg aaagaaatga
    11401 taaaatatgc tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc
    11461 gtcagaaaat tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc
    11521 gaagggctag aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg
    11581 aacgataata ttgcggccca aaaaacgtcg caagaattaa atgaattaaa aaacacttac
    11641 aataccttcc ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg
    11701 gctgaagacg agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata
    11761 atgaccagct acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc
    11821 ttggaaatcc atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt
    11881 cgtatgtata ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa
    11941 gcaggtgatg aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat
    12001 ggtctcctca agtcagaaat gggtgttcat cgcttagtgc gaatctcacc atttgactct
    12061 gccaaacgtc gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact
    12121 attgaagtgg aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc
    12181 ggtggacaaa acgtcaataa agtttcaaca ggtgtacgtt taacccacat tccaactgga
    12241 attgttgtcc aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag
    12301 atgttgcagg ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct
    12361 ctcaaaggtg agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg
    12421 ccttatacta tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt
    12481 atggatgggg acctagatgg ttttatcgat gcttatctca agtggcgaat tagctaagat
    12541 agaaaggaac tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg
    12601 gaacgactgc tctacgcggt gtttcggtca gcgttcaacc gggggaattt gcttacatcg
    12661 taggaccttc aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa
    12721 tcgataaagg aagtctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg
    12781 tcccgcttct acgtcgtagt gttggggttg tcttccaaga ttataaattg ttaccaaaga
    12841 aaactgtcta tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata
    12901 tcaaaagacg agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt
    12961 tcccaaatga actctcaggt ggggagcaac agcgaattgc gattgcgcgt gcaattgtaa
    13021 ataatcccaa agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat
    13081 gggaaattat gaatctcttg gaacggatta acctacaagg gacaactatt ttgatggcga
    13141 ctcataatag ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc
    13201 gtgtcgttcg tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc
    13261 catttatttg aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc
    13321 agttcagtca tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca
    13381 gcgaaactag ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat
    13441 gtggaagata atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac
    13501 cacaaggtat atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt
    13561 aaagaagaac aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa
    13621 ggagatgcca atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta
    13681 aaaactatag ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt
    13741 gccaatacag aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt
    13801 gctgctttgt taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt
    13861 atttcccgca gtcgcgaaat tcaaatcatg cgcttggtcg gagctaaaaa cagttatatc
    13921 cgtggaccgt tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct
    13981 gttttggtct ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg
    14041 caaaatctat ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt
    14101 gtgattgggg ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgagg
    14161 atttaggtaa aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga
    14221 gtttttgaaa agagatgcgt agaagaaaag agcttccaaa gaagtccccc agagaagact
    14281 tcttgatggc taatttgaac ttgaaatttg gcgcactaga aattttttag aaaaaagtgt
    14341 aattttttta taaaagcctt tacaaaaaaa attaaagtgg tataattaaa tcatagaagg
    14401 tgcaaacgtt ttcgtaaaac gtttgcctaa ataaaaataa aggagatttg aatgatgaaa
    14461 gatacattca aaaatgtctt gtctttcgaa ttttggcaaa aattcggtaa ggctttgatg
    14521 gtagttatcg cggttatgcc ggctgctggt ttgatgattt caatcggtaa gtctatcgtg
    14581 atgattaacc caacctttac accacttgtc atcacaggtg gaattcttga gcaaatcggt
    14641 tggggggtta tcggtaacct tcacattttg tttgccctag ccattggagg aagctgggct
    14701 aaagaacgtg ctggtggtgc tttcgccgct ggtcttgcct tcatcttgat taaccgtatc
    14761 actggtacaa tttttggtgt atcaggcgat atgttgaaaa atccagatgc tatggtaact
    14821 actttctttg gtggttcaat caaagttgct gattacttta tcagtgttct tgaagctcca
    14881 gccttgaaca tgggggtatt cgtagggatt atctcaggtt ttgtaggggc aactgcttac
    14941 aacaaatact acaacttccg taaacttcct gatgcacttt cattcttcaa cgggaaacgt
    15001 ttcgtaccat ttgtagttat tcttcgttca gcaatcgctg caattctact tgctgctttc
    15061 tggccagtag ttcaaacagg tatcaataac ttcggtatct ggattgccaa ctcacaagaa
    15121 actgctccaa ttcttgcacc attcttgtat ggtactttgg aacgtttgct cttgccattt
    15181 ggtcttcacc acatgttgac tatcccaatg aactacacag ctcttggtgg tacttatgac
    15241 attttaactg gtgcagctaa aggtactcaa gtattcggtc aagacccact atggcttgca
    15301 tgggtaacag accttgtaaa ccttaaaggt actgatgcta gtcaatacca acacttgtta
    15361 gatacagtac atccagctcg tttcaaagtt ggacaaatga tcggttcatt cggtatcttg
    15421 atgggtgtga ttgttgctat ctaccgtaat gttgatgctg acaagaaaca taaatacaaa
    15481 ggtatgatga ttgcaacagc tcttgcaaca ttcttgacag gggttactga accaatcgaa
    15541 tacatgttca tgttcatcgc aacacctatg tatcttgttt actcacttgt tcaaggtgct
    15601 gccttcgcta tggctgacgt cgtaaaccta cgtatgcact cattcggttc aatcgagttc
    15661 ttgactcgta cacctattgc aatcagtgct ggtattggta tggatatcgt taacttcgtt
    15721 tgggtaactg ttctctttgc tgtaatcatg tactttatcg caaacttcat gattcaaaaa
    15781 ttcaactacg caactccagg gcgcaacgga aactacgaaa ctgctgaagg ttcagaagaa
    15841 accagcagcg aagtgaaagt tgcagcaggc tctcaagctg taaacattat caaccttctt
    15901 ggtggacgtg taaacatcgt tgatgttgat gcatgtatga ctcgtcttcg tgtaactgtt
    15961 aaagatgcag ataaagtagg aaatgcagag caatggaaag cagaaggagc tatgggtctt
    16021 gtgatgaaag gacaaggggt tcaagctatc tacggtccaa aagctgacat tttgaaatct
    16081 gatatccaag atatccttga ttcaggtgaa atcattcctg aaactcttcc aagccaaatg
    16141 actgaagcac aacaaaacac tgttcacttc aaagatctta ctgaggaagt ttactcagta
    16201 gcagacggtc aagttgttgc tttggaacaa gtaaaggatc cagtatttgc tcaaaaaatg
    16261 atgggtgatg gatttgcagt agaacctgca aatggaaaca ttgtatctcc agtttcaggt
    16321 actgtatcaa gcatcttccc aacaaaacat gcttttggta ttgtgacgga agcaggtctt
    16381 gaagtattgg ttcacattgg tttggacaca gtaagtcttg aaggtaaacc atttacagtt
    16441 catgttgctg aaggacaaaa agttgcagca ggagatctcc ttgtcacagc tgacttgaat
    16501 gctatccgtg cagcaggacg tgaaacttca acagtagttg tcttcacaaa tggtgatgca
    16561 attaaatcag ttaaattaga aaaaacaggt tctcttgcag ctaaaacagc agttgctaaa
    16621 gtagaattgt aatatacttg aggttggaag ctgtattcca acctcttatt ttgggagaaa
    16681 agaatgaaat ttttaacact caatactcac agttggatgg agaaagaagc agaggaaaaa
    16741 ttccagattt tgcttgaaga tattcttgaa aaggactatg atttgatttg ttttcaagaa
    16801 atcaatcagg agatgacctc gtcagaggtg gaggttaatg acctttatca agctttgcca
    16861 gcagctgagc ctattcacca agaccattat gttagactct tggttgaaaa gttgttagag
    16921 caagggaaaa attactactg gacctgggct tataaccata tcggctatga ccgctaccac
    16981 gaaggtgtgg ctatcttgtc taaaacacct attgaagcca gagaaatttt ggtttcagat
    17041 gtggatgatc caacagacta tcatactcgc cgtgttgccc tagctgaaac tgtagtcgat
    17101 ggcaaggagc tagcagttgc cagtgttcat ctctcttggt gggataaagg tttccaagaa
    17161 gaatgggcac gatttgaggc tgtcttgaaa aaattgaaca agccactttt actagctgga
    17221 gattttaaca acccagcagg tcaggaaggt taccaagcta ttttagctag tccattaggc
    17281 ttacaagacg catttgaagt tgctcaagag aaaagtggta gctatactgt tccgcctgaa
    17341 attgatggct ggaaagggaa cactgaaccc cttcgaatcg attatgtctt tactaccaaa
    17401 gagttagcgg tggaaaattt acatgtcgta tttgatggta acaagagtcc acaagtgagt
    17461 gatcactatg gcttgaatgc ggtattaaat tggaaataaa atcaaaaaat gaaagggagc
    17521 gagtcctttc atttttttac aaaaatccct ataattacta ttttggagaa ggagtttttt
    17581 gtatatttgt atattatgag gaaatatcca caaataagtt tttaaaactt tacggtcaaa
    17641 aaaaaatcgc taatattaac tatttatgat agaatgaaaa ttatgaaata aaaggaggac
    17701 tggaatgttt tttcaggata gaaagataaa atattcaatc cgcaaattgt cagttgctac
    17761 tgtgtccctt gcgattggat ttttgtttgc gcctgcggcg cttggagtcg ctcaagtagc
    17821 tcaagctgat gagctttcaa ctactgaaca agtggagaat aaagcagaga atcaggtctc
    17881 ggtggtctct tttgcaacag agcaagaaga aagtgaaacg acttatgttg atacgacgaa
    17941 taaagtgact tggacatttg caggatacga taaagaaaaa attgttgtag gaaaaggcag
    18001 acagactttc cttggaagct gggtacctac tccaaatcca gaatatgtat tcaaatcatc
    18061 taaagcaggt gttccattac cacaaagtat tttgggaatg cttccaaagg atgaagcttc
    18121 ttataaggtt ggtgacacca ttgtagccaa acaacctgct gtagaatcag tagtcgaaga
    18181 agaaaaagac tatgtctgga ccttcaaagg ttatgatcag aagaatgcga cgtacaatgg
    18241 caagcgtgtg acatttacag gtatctggga agtaacgcct agaccgcatc atgtaagcta
    18301 tacttttgta agtgttacat ctgagaatgg taactctgtt tccgctaaac aaccagcgca
    18361 agcgacctac atagatagtg tgaacgacgg cacatggacc ttcaaaggtt atgacgcagc
    18421 tagcgccgtt gttaacaagg ctaatgtaga gtttgtaggt aagtgggaat tcaaggcgaa
    18481 tccgacaaat gcggaaactt atacgcctca ggtaacggaa gaaactatca aggttggaca
    18541 aacacctgac ttgaccgata atgtgacgaa cttgccaaac cttccagctg gcactaaggt
    18601 agttgatatt acacctgcag gtcaaattga cacaacaaaa ccaggtactt acacaggtaa
    18661 ggtgcgagtt gactatccag atggttcttc tactgaggtg tctgtatcag ttaatgtttt
    18721 gcctgcacca gaaactcaaa cctataaggt gacttaccgc ttcgaaagtg caaccgcaga
    18781 caacaacttg ccagcagaag tattgagctt actcccacaa agtggaacat atactacaag
    18841 ttcttctgta gcgattgctt tcgttccaga agcaccgatg cctgtagaag ttgctgttgc
    18901 aaatggtaca tggacattct taggttacca agaagtggaa gaggggtcaa atctaacctt
    18961 cgtcggtaag tgggtctttg aagctaagca agatcctagt ccacaaccac agccggcacc
    19021 aacgccacaa ccagcacctc aaccaaatcc agttccacca gtgaatccag ggggagagca
    19081 gggaactgat aataatcagg cagatagaca tcaatcagaa caaccaagaa aacctgaaga
    19141 gaatccacca gctcaaggtc cagcaggaga aaaggaaaaa caagctacat tgccaaatgc
    19201 gggtagtact gcaccacttt cactcactgg actagtgact agctctcttc ttgcaggtct
    19261 agcagtgttg cttcttggtc gcaagcggaa agatgattaa gtactaattt ttctagaagc
    19321 tttcgacttt tacaagattc ttaagcaact gtttgactgt ttgacaaatc tatctgagtc
    19381 gcaagcttta aggctgagag aagaccaatt tggtcttctc tttttgcatt ttggaaagaa
    19441 agcaaatgta tgatttttag tataagagag gtatagtaga ttgaaactag aatagtacac
    19501 ctctgcttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt catcctattc
    19561 ttatttcatt ttactatatt tagagaaatt gagagccttg tgataggtca gatgagtcgg
    19621 actactgaaa atgttcaaaa tatggtataa ttgataagat agatagaatc gaggatgtta
    19681 tgtcatttac gaaatttcaa tttaaaaact atattagaga agccttgaag gagttaaaat
    19741 ttacaactcc aacagaggtg caagacaagt tgattcctat tgttttggca ggtcgtgacc
    19801 tagtaggaga atcaaaaaca ggttcaggta agactcacac tttcttgtta ccgattttcc
    19861 agcaattaga tgaagctagc gatagtgtac aagcagtgat tactgcaccg agtcgtgagt
    19921 tggctactca aatttaccaa gtagcgcgtc agatttcagc tcactcagat gtcgaagttc
    19981 gtgtggttaa ttatgtgggt ggtacggata aggctcgcca gattgagaaa ttggcaagca
    20041 atcagcctca tattgttatt ggaacaccag gccgtatcta cgacttggtt aaatctggtg
    20101 atttagctat tcataaagcc aagacatttg ttgttgatga agcagatatg accttggata
    20161 tgggattctt ggaaactgtt gataagattg ctggcagtct tccaaaagac ttgcaattca
    20221 tggtcttctc agcgactatc ccacaaaaac tgcaaccatt cttgaaaaaa tacttatcaa
    20281 atcctgttat ggagaaaatt aagactaaaa cggttatttc tgacaccatt gataattggt
    20341 tgatttcgac caagggacat gataagaatg ctcaaattta ccagttgact cagttgatgc
    20401 agccgtattt ggcaatgatt tttgttaaca ctaaaacgcg tgctgatgaa ttgcattcat
    20461 atctgactgc tcaaggcttg aaggttgcaa aaatccatgg cgatattgcc cctcgtgaac
    20521 gcaagcgaat catgaatcag gtgcaaaatc tggattttga gtatattgtc gcaacagatt
    20581 tggcagcgcg tgggattgac attgaaggtg tcagccatgt catcaatgat gccattccgc
    20641 aagacttatc tttttttgtt caccgtgttg gccgtactgg acgaaatggc ctaccaggta
    20701 cagctattac cctttatcag ccaagtgatg actcggatat ccgtgagttg gagaaattgg
    20761 gaatcaagtt tagtcctaag atggtcaaag acggggaatt tcaagatacc tatgaccgtg
    20821 atcgtcgtgc taaccgtgag aaaaaacaag ataaacttga tatcgaaatg attggtttgg
    20881 ttaaaaagaa aaagaaaaaa gtcaaaccgg gttataagaa gaaaattcaa tgggcggttg
    20941 atgaaaagcg ccgtaaaacc aagcgtgctg aaaatcgcgc tcgcggtcgt gcagagcgta
    21001 aagctaaacg ccaaacattt taatagaaat tgttggagta ttgagctcca acttttttat
    21061 ttatgagaac gaactatcta aaccgaaaca ctacattaaa gactgcaaat tgcgattaaa
    21121 aatggtataa tgataaagtt atatagtccc gataagatgg taggtattta ttacaaagag
    21181 ttttcctatc agtactttgt aactctataa caatattttt taagggggga catttttatg
    21241 tcagaacgta aattattcac gtctgaatct gtatctgagg ggcatccgga taagattgca
    21301 gaccaaattt cagatgcgat tttggatgct attttagcaa aggatccaga ggcgcacgtt
    21361 gctgctgaaa cagctgtata tactggttct gtccacgttt ttggtgaaat ttctacaaat
    21421 gcctatgtgg atattaaccg tgtggttcgt gataccattg cagagattgg ttataccaat
    21481 acagaatatg gattttctgc tgagacggtg ggagtacacc catctttggt ggaacaatct
    21541 cctgacatcg ctcaaggtgt taacgaagcc ttggaggttc gtggaaatgc tgatcaagat
    21601 ccactggact tgattggagc aggtgaccaa gggctcatgt ttggatttgc agtagatgaa
    21661 acagaagagc tcatgccatt gccaattgca ctcagtcata aattggttcg tcgtctggca
    21721 gaacttcgta agtctggaga aattagctat ctccgtccag atgcaaaatc acaagttaca
    21781 gttgagtacg atgaaaatga tcgtccggta cgtgtagata cagtcgttat ttctactcag
    21841 catgatccag aggccactaa tgaacaaatc catcaagatg tgattgacaa ggtcatcaaa
    21901 gaagttattc catcttctta tcttgatgat aagacaaaat tctttatcaa tccgacaggt
    21961 cgttttgtaa tcggtggtcc tcaaggggac tcaggtttga ctggtcgtaa gattattgta
    22021 gatacttatg gtggctactc tcgtcatggt ggtggtgcct tctctggtaa agatgcgact
    22081 aaggtggatc gttcagcctc ttatgcggct cgctatattg ccaagaatat cgttgcagca
    22141 ggccttgcta agaaggcaga agtgcagttg gcctatgcta tcggtgttgc gcaacctgtt
    22201 tctgttcgta tcgatacttt cggtacagga acagtagctg aaagtcaact tgaaaaagcg
    22261 gctcgtcaaa tctttgacct tcgccctgca gggattatcc aaatgctgga cctcaagcgt
    22321 ccaatttacc gtcaaacatc ggcttacggt cacatgggac gtacagatat tgatcttcca
    22381 tgggaacgtt tggataaggt agatgctttg aaagaagcag taaaataaga ttttaagagg
    22441 ggaacgtcct ctctttttta tagtttttaa ctatattggg atactgttct gaaaatccat
    22501 tttgcgaaag tagagattta catgtatagt agattgaaac tagaatagta cacttcaact
    22561 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttctcctg ttcttgtttc
    22621 attttactat atttctttaa aaatgataaa ggttgagatt tctcctcgta atagataatc
    22681 atggagatat ttcaatccaa agttttattc gttatcactt gactattgca aggttttcta
    22741 gagcaacaga gtcatggaat ggactcatgg ttgagatttc tccttgttgc ttggacttca
    22801 ttcaaaagtc tgttacccaa gccttgttca aacttccaat atactagctg tttccatagc
    22861 atgacttctg tactagactt tcttttccga ataaatagat agaaccacag aatctagtaa
    22921 acctagaatt aaaattatgg tataatatta gcaataaaag aaatctggag gattagaatc
    22981 atggtatcaa cgaaaacaca aattgctggt tttgagtttg acaattgctt gatgaatgca
    23041 gcaggtgtgg cttgtatgac gatagaggag ttagaagagg tcaaaaactc agcggcagga
    23101 acctttgtta ctaagacagc gaccttggac ttccgtcagg ggaatcctga gccacgctac
    23161 caagatgttc cacttggttc catcaactct atgggcttgc caaataatgg cttagactat
    23221 tatttggatt atcttttaga tttgcaggaa aaagagtcga accgaacttt cttcttatct
    23281 ctggtcggca tgtctccaga ggaaacccat actattttga aaaaagtcca agagagtgat
    23341 tttcgtggtc tgactgagct aaatctttcc tgtccaaatg ttccaggtaa acctcagatt
    23401 gcctatgatt ttgagacaac agaccggatt ttggcagaag tgtttgctta cttcaccaaa
    23461 cctcttggaa ttaaattgcc accttatttt gatattgttc actttgatca agcggcagct
    23521 attttcaaca aatatccgct caagtttgtc aactgcgtta actctatcgg aaacggcctc
    23581 tatatagaag acgaatctgt cgttattcgg cctaagaatg gttttggtgg aattggtgga
    23641 gaatacatca aaccgactgc tctagccaat gttcacgcct tttatcaacg tttaaatcct
    23701 caaatccaaa ttatcggaac aggtggcgtt ctgactggtc gagatgcctt tgaacacatc
    23761 ctctgtggag caagtatggt gcaggtggga acgacccttc acaaagaagg cgtcagtgct
    23821 tttgaccgca ttaccaatga actgaaagca atcatggtgg aaaaaggcta cgagagctta
    23881 gaagatttcc gtgggaaatt gcgctatatt gactaaatta aatcgaaaaa tctgaagaaa
    23941 ggagagacga tgctagccat tgaagaaagt cagaagttga ctttatcaaa tttaccgagc
    24001 ctgagcctat ttacagggac agatcagggg cagtttgaag tgatgaagag tcaagtgttg
    24061 aaacagattg ggtatgattc tgctgacctc aactttgcct actttgatat gaaagaagta
    24121 gtttacaagg atgtggaact ggagttggtc agccttcctt tctttgcgga tgagaaaatc
    24181 gtgatattag attattttat ggatatcacg actgctaaga aacgcttttt gacagatgat
    24241 gagcttaagt catttgagga ataccttgac aatccttctc caacaaccaa gttgataatc
    24301 tttgcagaag gaaagctgga tagcaaaaga cggttagtca aattacttaa gcgtgatgcc
    24361 aaggccttcg atgcagtaga agtaaaagaa caagaattgc gccagtactt ccaaaagtgg
    24421 agtcagaaac aaggtctgca gtttaccaat cattcttttg aaaatctcct catcaagtcg
    24481 gggtttcaat ttagcgaaat ccagaaaaat cttctctttt tacagtccta taaggcggat
    24541 tctgttatcg aggaagagga tattgttaac gcaattccca agaccttgca ggacaatatt
    24601 tttgatttaa ctcagtttat tctgactaaa aagatggatc aggcgcgcga tttggtgaga
    24661 gacttgacct tgcaagggga agatgaaatc aaactgattg cagtcatgct gggacaattt
    24721 cggactttta ctcaggtgaa gattttggcg gagtctggcc aaacagaatc gcagattgca
    24781 agtagtttag gtagttatct gggacgtaac ccaaatcctt atcaaatcaa gtttgcatta
    24841 agagattcga gaggactttc tttgagcttt ctgaagcaag ctatttccta tttgattgag
    24901 acagactatc agattaagac aggtctttat gaaaaaggtt tcctttttga aaaggcactc
    24961 ttacagattg ctagtcaggt caattgacat ttgttgaaac tacaacccgc atcaggatta
    25021 tcttgtcgtg ggtttcttgc tgatttgctt attttattag aaagtatgtc agtgatttcc
    25081 ttatctataa gcaaaaaact tgaaaaaagt gaatgtttgc gttatcattt tcatatagaa
    25141 agaaaaagag gtattacaga tggctattat cttaccagaa cttccatatg catacgacgc
    25201 tttggaacca tacatcgatg cggaaacaat gcatttgcac catgacaaac accatcaaac
    25261 ttatgtcaac aatgccaatg cagctttaga aaaacaccct gaaatcggtg aagaccttga
    25321 agccttgctt gctgatgtag aatctatccc agctgatatc cgtcaagcac ttatcaacaa
    25381 tggtggcgga cacttgaacc acgctctttt ctgggaattg atgactcccg agaaaacagc
    25441 tccttcagca gaactggcag cagcaatcga tgcaacattt ggttcatttg aagaattcca
    25501 agcagccttc actgcagcag caacaactcg ttttggttca ggttgggcat ggttggttgt
    25561 caacaaagaa ggaaaacttg aagtgacttc aacagcaaac caagacacac caatctcaga
    25621 aggtaaaaaa ccaatcttgg gcttggacgt ttgggaacat gcttactacg tgaaataccg
    25681 caacgtgcgt cctgactaca tcaaagcttt cttttcagta atcaactgga ataaagtaga
    25741 tgaattgtac gcagctgcta aataatgata gttggaggga agaattgttc ttctcttttt
    25801 taggttataa gattctggtc tgacaaaatc gttagacttt tttcattttt atgagaaacg
    25861 tgctaactgc tagaaattat ggtagaataa agtattaagt aatcgtgaaa aaaggatatc
    25921 tatgcgaaaa gaaattgcac ctgaattata caactataac aagtttcctg gtccggagtt
    25981 ccatttacac ggggacaagg tcgaaacgga agggatagct ttttccttgg ttgaaaatat
    26041 caaggatgcc tttgatgtga cgacttttaa tcagcgtttt tcagaagtat taaccaagtt
    26101 tgattatatc gtgggggact ggagcaacga acagcttcgc ctacgaggtt tttacaagga
    26161 tgaccgaaca gaagaaaaac ttgaaaaaat cagtcgttta caagactacc ttttagagta
    26221 ttgtagttat ggttgtgcct attttgtctt agaaaatgaa gcccctaagc gagcatcatt
    26281 tgacaagaaa atgcgtaaga cggaagaaga aacaccttct agaaaaggaa agaaaccgac
    26341 tcaaactaaa cgaaagtcga atgcagataa gaagaataga cgtcgtcaga aagaccagca
    26401 ttctcagaaa gaggacaagg gacaacgtca ttttgtcatt cgtcagaagt gagtagagaa
    26461 taaggagaag aaatgaaacc ctcaattcat agtttggcac atcaaaccat gcaggaatgg
    26521 gtattggagc agggagaaaa gaaattccgt gcagatcaaa tctgggaatg gctctaccgt
    26581 aaacgtgtgc agtcatttga agaaatgacc aacctttcca aggatttgat tgctaagctc
    26641 aatgaccagt ttgtggtcaa tcccttgaaa caacgtattg tgcaagagtc tgctgatggt
    26701 acagtcaaat atctctttga attgcccgac ggtatgttaa ttgagactgt actcatgcgt
    26761 caacactatg gtttatctgt ctgtgtgacc actcaggtcg gctgtaatat cggttgtacc
    26821 ttctgtgcct ctggtttgat taagaagcaa cgtgacctca ataacggtga aatcgtagcg
    26881 caaatcatgc tggttcagaa atactttgat gagcgtggtc aggatgaacg cgtcagccat
    26941 atcgttgtca tgggaatcgg tgagcccttt gataattaca acaatgtctt gaatttcttt
    27001 cgtactatca atgatgataa aggaatggct atcggtgctc gtcacatcat ggtttcaacc
    27061 tcaggtttgg ctcataaaat tcgtgacttt gctgatgaag gggttcaggt caatcttgcc
    27121 gtgtctcttc acgcacccaa caatgaactt cgttcaagta tcatgaagat taaccgtgcc
    27181 tttccgattg aaaaactctt tgctgctatt gaatactaca ttgagacaac aaaccgtcgt
    27241 gtaacctttg aatatatcat gcttaatgaa gtcaacgacg gtgtagaaca agccttggaa
    27301 ttggctgaat tgctcaagaa catcaagaaa ttgtcttatg taaacttgat tccttataac
    27361 ccagttagtg agcatgacca atatagccgt agtcccaaag agcgcgtgtt ggctttctat
    27421 gatacgctta agaaaaaagg ggtcaactgt gtggttcgtc aagagcatgg tacagatatt
    27481 gacgcagctt gtggacaatt gcgctctaac acaatgaaac gtgaccgcca gaaggcagtc
    27541 gcagcggtaa atccttaatg atgaagaaga cttataatca tattttggtc tggggagtca
    27601 ttttctatag catttgcatt gtctgttttt gctttactcc tcaagaacaa tctaccgtgg
    27661 gagtgggaac tccaggtatt cagcatcttg gacgcctggt ttttcttttg actcctttca
    27721 attctctctg gaaactgggc gaagtgagtg acattggaca attatgttgg atttttttac
    27781 aaaatgtcct caatgtcttc ttgttttttc ctctgatttt ccaactcctt tatctatttc
    27841 caaatttgcg gaaaacaaaa aaggtccttc tttttagttt tcttgtgagt cttggaatcg
    27901 agtgtacgca attaatcttg gactttttct ttgatttcaa tcgcgtcttt gagattgatg
    27961 atttgtggac caatactttg ggtggctatc tggcttggct cctttataaa cgattacata
    28021 aaaacaagat aaggaattaa aatgagtatt ttagaagtta aaaatctgag tcacggtttt
    28081 ggtgaccgtg caatttttga agatgtgtcc ttccgtctcc tcaagggaga acatatcggc
    28141 ctggtcggtg ccaatggtga aggaaaatca acctttatga gtatcgtgac tggtaaaatg
    28201 ctgccagatg aaggaaaggt tgagtggtcc aaatatgtga cggctggtta cttggatcag
    28261 cactctgtcc ttgctgaaag acagtcggtg cgtgatgttc tccgtacggc ttttgatgag
    28321 cttttcaaag ctgaagctcg tatcaatgac ctctatatga aaatggctga agacggcgcg
    28381 gatgttgatg ctctcatgga agaagtagga gaacttcaag accgtctgga gagtcgtgat
    28441 ttctatacct tggatgctaa gattgacgaa gtagcgcgtg ctcttggtgt tatggacttt
    28501 ggcatggata cggatgtaac ttctttgtca ggtgggcaaa gaaccaaggt gcttttggca
    28561 aaacttctcc ttgaaaagcc tgatatcttg ctgttggacg agccgaccaa ctacttggat
    28621 gctgagcata ttgattggct caagcgctat ctccaaaact atgagaatgc ctttgttctc
    28681 atttcgcacg atattccatt cctcaatgac gttattaata ttgtctatca tgtggaaaat
    28741 caacagctga cgcgttactc tggtgactac taccagttcc aagaagttta tgctatgaag
    28801 aaatctcagc tagaggcagc ctacgaacgc cagcagaaag agattgcaga cctcaaggac
    28861 tttgtggctc gtaataaagc ccgtgttgca actcgtaata tggctatgtc tcgtcaaaag
    28921 aaattggata agatggatat tatcgaactc caaagtgaga aaccaaaacc atcctttgat
    28981 ttcaaaccag ctcgtacacc agggcgcttt atcttccaag ccaagaactt gcaaattggt
    29041 tacgaccgtc ctcttactaa gcctttaaat cttaccttcg aacgcaatca aaaggttgcg
    29101 attattggtg ctaatggtat tggaaaaaca actctcttga agagtctctt aggcattatc
    29161 tcgccaatcg ctggggaagt ggagcgtgga gattatttag aacttggtta ttttgagcag
    29221 gaagtagaag gtggtaatcg ccaaactcct cttgaagctg tctggaatgc ctttcctgcc
    29281 cttaatcaag cagaagtccg tgcagccctt gcccgttgtg gtttgacaac caaacatatt
    29341 gaaagccaga ttcaagtatt atcaggggga gagcaagcca aggttcgttt ctgtctcttg
    29401 atgaatcgtg aaaacaacgt tttagtgctg gacgagccga ccaaccattt ggatgtggat
    29461 gcaaaggatg agctcaaacg cgctctcaaa gaatataggg gatctatcct tatggtctgc
    29521 cacgagccag acttttatga aggctggata gaccaaatat gggattttaa taatttaact
    29581 taagaatgag agtaaaagaa atacagtacc gaatgtggat actgtatttt tttggttttt
    29641 aagatttaaa atcgtagtct ttcaccactt cgatgctgtc gattgtgata gcagtagttg
    29701 gcttgtcttt ttcatctttt tcggccttag caatcttatc cacaacatcc ataccgtcaa
    29761 tcacttgacc aaagactggg tgtttgccat ctagactagg gtttccacct tctttgtagg
    29821 cttcgataat tttctgtgga tacttgcttg tagggagttt agaagaggta tctgtagagt
    29881 tttggttgat gaagaactgg ctgccattgg tgtttggttg accagtatta gccatagcaa
    29941 gagcaccgcg gatgttatac aaataaggag taatctcgtt cttgaaacca gttcctttgt
    30001 ctttagtctt atccttgtca tgccagatgg actgaccacc tgtaccgtcc ccttttggat
    30061 ctccagtttg gaccataaag ccatcgatga cacggtggaa ggtaataccg ttatagtagc
    30121 cttctttggc gtgagtgagg aaattttcaa ccgctagagg agcgagttta gggaagagtt
    30181 taatgcgaat atcaccttgg cttgtgtgga gaatcacttc ggcttcatct tcagcaactt
    30241 ccttagatag ttgtgggaaa ttggcgtttt cgtttgttaa agcatcgttt aactccttgg
    30301 cagattgggc agctactttg gaactttctt cagcagcaag actggaatca acataatcat
    30361 caccacgcag actgcgttgg acgctgctac acccagctag ggctacagta gacagtaaaa
    30421 gaagggttgc tagttttttc attctgatcc tctcaaaaat tcatttatac cattgtaccc
    30481 taaaaggaag tggatttcaa atattagtag tttacagaac tggcttagat aatagttggg
    30541 atttcatcat catcttgaaa atcttcaact tcttccccag taaatggttt ctgaaaagaa
    30601 cttagtataa aacgggcaat tggttcaaca attaggaaat ttagaggaag agctgcaata
    30661 aagtttacga accaggcgct tagataggta ttgggtgtga tagcaatgcc attgtaaaaa
    30721 agtccataga aggacatgaa agtgaccagc taattcttct tctgaaaaat gctcatgaag
    30781 ggcttgatcc aggtcgcaag atttcatttt agaaagtcca gtaactccct tcttcttcag
    30841 gatattcttt ttcatggtat tatttagatg gtcaagtgag tcaaaagaag tttcgacaag
    30901 agcataccct ttttctacca gagtttttag atgtttaggg gcatcaatac cataagtgta
    30961 ctcaaagtat tttggaaacc agatcttaag aaagctcgta aagctagcca gttctctaaa
    31021 cgctaagaac tagctacaac acag
//
LOCUS       DAVLTM010000010        31015 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLTM010000010 DAVLTM010000000
VERSION     DAVLTM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.010"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VYN16_000400"
     rRNA            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VYN16_000400"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(173..3074)
                     /locus_tag="VYN16_000401"
     rRNA            complement(173..3074)
                     /locus_tag="VYN16_000401"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3199..3271)
                     /locus_tag="VYN16_000402"
     tRNA            complement(3199..3271)
                     /locus_tag="VYN16_000402"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3236..3238),aa:Ala,seq:tgc)
     gene            complement(3323..4868)
                     /locus_tag="VYN16_000403"
     rRNA            complement(3323..4868)
                     /locus_tag="VYN16_000403"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(5120..5191)
                     /locus_tag="VYN16_000404"
     tRNA            complement(5120..5191)
                     /locus_tag="VYN16_000404"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5156..5158),aa:Glu,seq:ttc)
     gene            complement(5285..5764)
                     /locus_tag="VYN16_000405"
     CDS             complement(5285..5764)
                     /locus_tag="VYN16_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1402987.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            complement(5886..7844)
                     /gene="ftsH"
                     /locus_tag="VYN16_000406"
     CDS             complement(5886..7844)
                     /gene="ftsH"
                     /locus_tag="VYN16_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1402988.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(7860..8402)
                     /gene="hpt"
                     /locus_tag="VYN16_000407"
     CDS             complement(7860..8402)
                     /gene="hpt"
                     /locus_tag="VYN16_000407"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1402989.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(8406..9683)
                     /gene="tilS"
                     /locus_tag="VYN16_000408"
     CDS             complement(8406..9683)
                     /gene="tilS"
                     /locus_tag="VYN16_000408"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1402990.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(9680..10948)
                     /locus_tag="VYN16_000409"
     CDS             complement(9680..10948)
                     /locus_tag="VYN16_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1402991.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGELTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(11068..11436)
                     /locus_tag="VYN16_000410"
     CDS             complement(11068..11436)
                     /locus_tag="VYN16_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1402992.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(11429..11695)
                     /locus_tag="VYN16_000411"
     CDS             complement(11429..11695)
                     /locus_tag="VYN16_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1402993.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(11753..15262)
                     /gene="mfd"
                     /locus_tag="VYN16_000412"
     CDS             complement(11753..15262)
                     /gene="mfd"
                     /locus_tag="VYN16_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1402994.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLMSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(15263..15832)
                     /locus_tag="VYN16_000413"
     CDS             complement(15263..15832)
                     /locus_tag="VYN16_000413"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1402995.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDEYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(15903..17018)
                     /gene="ychF"
                     /locus_tag="VYN16_000414"
     CDS             complement(15903..17018)
                     /gene="ychF"
                     /locus_tag="VYN16_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1402996.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(17138..18304)
                     /locus_tag="VYN16_000415"
     CDS             complement(17138..18304)
                     /locus_tag="VYN16_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1402997.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGATVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITVPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(19010..19678)
                     /locus_tag="VYN16_000416"
     CDS             complement(19010..19678)
                     /locus_tag="VYN16_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV1402998.1"
                     /translation="MKFCPECGNSVVGCKFCPNCGYSINASLKTTPQKVATPSSRPST
                     VRSKRTDKVGPLEIDKNHRTYRIHGARKAKGSSGLIGGTAKVVGKTTLALGTGGLSLI
                     PSLIKKDKNDTDWYSFDDLVSYELVINDQSVVSGGVGQALVAGAVFGVIGAVAGGIVA
                     KRKATTKILNMTIRITSNDFTKPVVFIDLIKKPVKNTSKEYKEAIENAQKIIGALDVI
                     THNS"
     gene            complement(19697..20413)
                     /locus_tag="VYN16_000417"
     CDS             complement(19697..20413)
                     /locus_tag="VYN16_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1402999.1"
                     /translation="MADNIKRLLSAKGLNPRQLAIALDFKYTTVNDWVNAKTYPRIDK
                     IEMLANFFNVSKSDLVENKNAETPTTSPIQSIYDQLTPPRQEKALTYLKKQLLEQKNE
                     NIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNIEKCFYTDEIPDDYDAIAYV
                     VGNSMEPKIKNGDYLFIKNTPQVDYNTIGIFQVDGANYVKKLRQGYLESLNPECADIQ
                     LDESNDIRTIGEVVGIYREK"
     gene            20617..20808
                     /locus_tag="VYN16_000418"
     CDS             20617..20808
                     /locus_tag="VYN16_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403000.1"
                     /translation="MRLTLKALRANSNMKQSEVAQKLGISTTTWSKWENGKSFPDVAQ
                     VKEIEKLFGVAYDDIIFLS"
     gene            20883..21260
                     /locus_tag="VYN16_000419"
     CDS             20883..21260
                     /locus_tag="VYN16_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403001.1"
                     /translation="MLYHWGLTFSEEISDLEHKGENNMNNKQVYQAQTTDHGIARALG
                     EEIRMITDVEETTYNGHGEIIVEYTSDVALTKLFDRIKALYEKGESYETKPFLHFKVL
                     EDDIEPSPNGVFTAKEVAEFLGI"
     gene            21282..21908
                     /locus_tag="VYN16_000420"
     CDS             21282..21908
                     /locus_tag="VYN16_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV1403002.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVRKFKMNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIENWEQAPKHAHSTMNNLLLKAVTDRNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            22178..22351
                     /locus_tag="VYN16_000421"
     CDS             22178..22351
                     /locus_tag="VYN16_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1403003.1"
                     /translation="MTDKIKKVGRPKGENSNTVKLTVRVSEQLNSKLEEYGKKNGLTK
                     PETLRKAFENLTK"
     gene            22505..22912
                     /locus_tag="VYN16_000422"
     CDS             22505..22912
                     /locus_tag="VYN16_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403004.1"
                     /translation="MGKQVFTQEILRNIQEENGIITVDLILDALPTWSEKAIKGRLSN
                     WRYRKVIDYRVEDGEFSEIFLLRSKQEIKEEVSAGQRLKMDLYFRQVLALTGIIESNT
                     SKDNDKTKAIELQQKAMRAIPDDIYKELSEIYE"
     gene            23406..23906
                     /locus_tag="VYN16_000423"
     CDS             23406..23906
                     /locus_tag="VYN16_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403005.1"
                     /translation="MIADQLKAISTNLEEIQEILVGDGTALAKREISRVLNDLDDVYN
                     ELTSTEYREQQETLKEQTERMKQRVVAEVIGGLEEREESYYRNYWGDTKFIKLLSNFD
                     GFLFLNTYLAEITKENTYPMEQSQLLNYVWEVIAVDIAKKKRGKKNVLDSWTDSSLYT
                     RGMMID"
     gene            23918..24058
                     /locus_tag="VYN16_000424"
     CDS             23918..24058
                     /locus_tag="VYN16_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403006.1"
                     /translation="MTLDLGKMTQAEFDEVMADIKARNPNLLQFITDFLDRKVTPGEV
                     DD"
     gene            24120..24335
                     /locus_tag="VYN16_000425"
     CDS             24120..24335
                     /locus_tag="VYN16_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403007.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            24332..24628
                     /locus_tag="VYN16_000426"
     CDS             24332..24628
                     /locus_tag="VYN16_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403008.1"
                     /translation="MSYIVKIYLDSEKIPDEPYFTKEIFLATCMGNATKLAVTMGLLT
                     KQVLDLETKTAILKALDTIIETQKAILQGVASGQITFVNKSKREEELDEPIRER"
     gene            24606..24947
                     /locus_tag="VYN16_000427"
     CDS             24606..24947
                     /locus_tag="VYN16_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1403009.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            24940..25213
                     /locus_tag="VYN16_000428"
                     /pseudo
     CDS             24940..25213
                     /locus_tag="VYN16_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25210..26070
                     /locus_tag="VYN16_000429"
     CDS             25210..26070
                     /locus_tag="VYN16_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV1403010.1"
                     /translation="MTIYEARGFQSNLVYPFDKIEPFQYIERFKPLVVPESADTEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYLTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            26150..27604
                     /locus_tag="VYN16_000430"
     CDS             26150..27604
                     /locus_tag="VYN16_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV1403011.1"
                     /translation="MWELYSRIWELGDQWRKENSYIVNEGKKNERVEIPRPSVAIVAK
                     ALQEICHFTFIGEGVISDISKLYLYHLDLGHYVSSNDIFRKLLLKYDSRLTSNKFFLE
                     LISYIRTETKMKPPLDDYRYIPVANGVYNIKTHKLEEFSPNFVITSKIQTEYKPCARK
                     PILDGWFDFDRWLEALAVNDKEVVALLWQVINEAINPNRTRKKMVLMVGDGNNGKGTF
                     QSLLENLIGRSNISNLKPDQFGKEFYLGALEGKVCNIGDDISNKYLDEVSDLMSVISG
                     DPVQVNKKSLQPVEARFRLLCIFSGNDLPRARNKTMGWYRRLCIIPFRADFNGQKERH
                     EIKDRFIKNKELLEWVLFKVLNMPDFDSFIEPEAVQKMLSKYKNDNDYIKVWVENFYI
                     PNGWHEINHVPMFIARNKLKEFAEDIGIDKPKLGQFSNAVMAELEKTTSYKYSAKNGT
                     VSSKFYDVLDPQGFQRKRFVKGIWGIHLEDEDLG"
     gene            28205..28729
                     /locus_tag="VYN16_000431"
     CDS             28205..28729
                     /locus_tag="VYN16_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403012.1"
                     /translation="MTLKTFSDKAKTFNFTYEFKDQDTAQVAGSALMGYMIGTYVVPS
                     ISITYKNKGTLVAEYVEDHKLNKTFKRICDGFKDYYKQPEGDEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELKLLDYAERLLSDKPILMNSMTAFGTLEVLGNESINLFQKLD
                     VEGEYKGLADYSGQ"
     gene            28792..29058
                     /locus_tag="VYN16_000432"
     CDS             28792..29058
                     /locus_tag="VYN16_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403013.1"
                     /translation="MVRSLKARLEKLEEYAGKTEYATTDECISLYVDCLRFAFQQDEI
                     KIDMDDFIKTPFKDIKLADYKKSKKRMDELREMTGISEFSELEQ"
     gene            29109..29450
                     /locus_tag="VYN16_000433"
     CDS             29109..29450
                     /locus_tag="VYN16_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1403014.1"
                     /translation="MANYLMTGNVKKASELSEITRKTAYNYLNDATFKRIYRERRSEQ
                     LKESTTLLQNASVEAVNVLREIMLDKTVSPYARQQSAQSILNMAYKAVEMVEVVEQLE
                     ILEAKILDEGD"
     gene            29437..29688
                     /locus_tag="VYN16_000434"
     CDS             29437..29688
                     /locus_tag="VYN16_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403015.1"
                     /translation="MKAIKNRLNKLNKLAVVDEVDTVFLTQIDTGHFEVRYRIGGKNP
                     KDIIEVYPTKEEAEKAISEIKNTKGLRIFWDNLALLEDN"
     gene            complement(30127..30468)
                     /locus_tag="VYN16_000435"
                     /pseudo
     CDS             complement(30127..30468)
                     /locus_tag="VYN16_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
BASE COUNT         9458 a         6353 c         5691 g         9513 t
ORIGIN      
        1 cacagggggc aacccccaac tacttccggc gttctagggc ttaacttctg tgttcggcat
       61 gggtacaggt gtatctccta ggctatcgtc acttaactct gagtaatacc tactcaaaat
      121 tgaatatcta ttcaacttaa gaaaaccgtt cgctttcata ttctcagtta ctttggataa
      181 gtcctcgagc tattagtatt agtccgctac atgtgtcgcc acacttccac ttctaaccta
      241 tctacctgat catctctcag ggctcttact gatatataat catgggaaat ctcatcttga
      301 ggtgggtttc acacttagat gctttcagcg tttatccctt ccctacatag ctacccagcg
      361 atgcctttgg caagacaact ggtacaccag cggtaagtcc actctggtcc tctcgtacta
      421 ggagcagatc ctctcaaatt tcctacgccc gcgacggata gggaccgaac tgtctcacga
      481 cgttctgaac ccagctcgcg tgccgcttta atgggcgaac agcccaaccc ttgggaccga
      541 ctacagcccc aggatgcgac gagccgacat cgaggtgcca aacctccccg tcgatgtgaa
      601 ctcttggggg agataagcct gttatcccca gggtagcttt tatccgttga gcgatggccc
      661 ttccatacgg aaccaccgga tcactaagcc cgactttcgt ccctgctcga gttgtagctc
      721 tcgcagtcaa gctcccttat acctttacac tctgcgaatg atttccaacc attctgaggg
      781 aacctttggg cgcctccgtt accttttagg aggcgaccgc cccagtcaaa ctgcccgtca
      841 gacactgtct ccgataggga tcacctatct gggttagagt ggccataaca caagggtagt
      901 atcccaacag cgtctccttc gaaactggcg tcccgatctc ttagactcct acctatcctg
      961 tacatgtggt acagacactc aatatcaaac tgcagtaaag ctccatgggg tctttccgtc
     1021 ctgtcgcggg taacctgcat cttcacaggt actaaaattt caccgagtct ctcgttgaga
     1081 cagtgcccaa atcattacgc ctttcgtgcg ggtcggaact tacccgacaa ggaatttcgc
     1141 taccttagga ccgttatagt tacggccgcc gtttactggg gcttcaattc ataccttcgc
     1201 ttacgctaag cactcctctt aaccttccag caccgggcag gcgtcacccc ctatacatca
     1261 tcttacgatt tagcagagag ctgtgttttt gataaacagt tgcttgggcc tattcactgc
     1321 ggctgactta aagtcagcac cccttctccc gaagttacgg ggtcattttg ccgagttcct
     1381 taacgagagt tctctcgctc acctgaggct actcgcctcg actacctgtg tcggtttgcg
     1441 gtacgggtag agtatgttta aacgctagaa gcttttcttg gcagtgtgac gtcactaact
     1501 tcgctactaa acttcgctcc ccatcacagc tcaatgttac agatataagc atttgactca
     1561 tatcacacct cactgcttag acagactctt ccaatcgtct gctttagtta gcctactgcg
     1621 tccctccatc actacatact ctagtacagg aatatcaacc tgttgtccat cggatacacc
     1681 tttcggtctc tccttaggtc ccgactaacc cagggcggac gagccttccc ctggaaacct
     1741 tagtcttacg gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact
     1801 tctatgcgtt ccagcactcc tcacggtata ccttcatcac acatagaacg ctctcctacc
     1861 atacctataa aggtatccac agcttcggta aattgtttta gccccggtac attttcggcg
     1921 cagggtcact cgactagtga gctattacgc actctttgaa tgaatagctg cttctaagct
     1981 aacatcctag ttgtctgtgc aaccccacat ccttttccac ttaacaatta ttttgggacc
     2041 ttagctggtg gtctgggctg tttccctttc gactacggat cttagcactc gcagtctgac
     2101 tgccgaccat aattcattgg cattcggagt ttatctgaga ttggtaatcc gggatggacc
     2161 cctcacccaa acagtgctct acctccaaga atctctaatg tcgacgctag ccctaaagct
     2221 atttcggaga gaaccagcta tctccaagtt cgtttggaat ttctccgcta cccacaagtc
     2281 atccaagcac ttttcaacgt gccctggttc ggtcctccag tgcgtcttac cgcaccttca
     2341 acctgctcat gggtaggtca catggtttcg ggtctacgtc atgatactaa ggcgccctat
     2401 tcagactcgg tttccctacg gctccgtctc ttcaacttaa cctcgcatca taacgtaact
     2461 cgccggttca ttctacaaaa ggcacgctct cacccattaa cgggctcgaa cttgttgtag
     2521 gcacacggtt tcaggttcta tttcactccc ctcccggggt gcttttcacc tttccctcac
     2581 ggtactggtt cactatcggt cactagggag tatttagggt tgggagatgg tcctcccaga
     2641 ttccgacggg atttcacgtg tcccgccgta ctcaggatac tgctaggtac aaagactatt
     2701 ttaaatacga ggctattact ctctttggct gatcttccca aatcattctt ctataatctt
     2761 tgagtccaca ttgcagtcct acaaccccga agagtaaact cttcggtttg cccttctgcc
     2821 gtttcgctcg ccgctactaa ggcaatcgct tttgctttct cttcctgcag ctacttagat
     2881 gtttcagttc actgcgtctt cctcctcaca tccttaacag atgtgggtaa caggtattac
     2941 ctgttgggtt cccccattcg gaaatccctg gatcatcgct tacttacagc tacccaaggc
     3001 atatcgtcgt ttgtcacgtc cttcgtcggc tcctagtgcc aaggcatcca ccgtgcgccc
     3061 ttattaactt aaccttattt tctgaccttt cagtcataaa ctcttattaa tactacagcg
     3121 ttttcggttt attttcttgt tactatttga tatagatatt caattttcaa tgtgcattac
     3181 ttggtgatct ctcaccaatg gagcctagcg ggatcgaacc gctgacctcc tgcgtgcaaa
     3241 gcaggcgctc tcccagctga gctaaggccc cacaagacct ctcaagacta aacaagacca
     3301 atgcgcagtt ccttatcctt agaaaggagg tgatccagcc gcaccttccg atacggctac
     3361 cttgttacga cttcacccca atcatctatc ccaccttagg cggctggctc cttacggtta
     3421 cctcaccgac ttcgggtgtt acaaactctc gtggtgtgac gggcggtgtg tacaaggccc
     3481 gggaacgtat tcaccgcggc gtgctgatcc gcgattacta gcgattccga cttcatgtag
     3541 gcgagttgca gcctacaatc cgaactgaga ctggctttaa gagattagct tgccgtcacc
     3601 ggcttgcgac tcgttgtacc agccattgta gcacgtgtgt agcccaggtc ataaggggca
     3661 tgatgatttg acgtcatccc caccttcctc cggtttatta ccggcagtct cgctagagtg
     3721 cccaactaaa tgatggcaac taacaatagg ggttgcgctc gttgcgggac ttaacccaac
     3781 atctcacgac acgagctgac gacaaccatg caccacctgt cacctctgtc ccgaaggaaa
     3841 gctctatctc tagagcggtc agagggatgt caagacctgg taaggttctt cgcgttgctt
     3901 cgaattaaac cacatgctcc accgcttgtg cgggcccccg tcaattcctt tgagtttcaa
     3961 ccttgcggtc gtactcccca ggcggagtgc ttaatgcgtt agctacggca ctaaaccccg
     4021 gaaagggtct aacacctagc actcatcgtt tacagcgtgg actaccaggg tatctaatcc
     4081 tgtttgctcc ccacgctttc gagcctcagc gtcagttaca agccagagag ccgctttcgc
     4141 caccggtgtt cctccatata tctacgcatt tcaccgctac acatggaatt ccactctccc
     4201 ctcttgcact caagttaaac agtttccaaa gcctactatg gttaagccac agcctttaac
     4261 ttcagactta tctaaccgcc tgcgctcgct ttacgcccaa taaatccgga caacgctcgg
     4321 gacctacgta ttaccgcggc tgctggcacg tagttagccg tccctttctg gtaagatacc
     4381 gtcacagtgt gaactttcca ctctcacact cgttcttctc ttacaacaga gctttacgat
     4441 ccgaaaacct tcttcactca cgcggcgttg ctcggtcaga cttccgtcca ttgccgaaga
     4501 ttccctactg ctgcctcccg taggagtctg ggccgtgtct cagtcccagt gtggccgatc
     4561 accctctcag gtcggctatg tatcgtcgcc ttggtgagcc gttaccccac caactagcta
     4621 atacaacgca ggtccatctg gtagtgatgc aagtgcacct tttaagcaaa tgtcatgcaa
     4681 catccactct tatgcggtat tagctatcgt ttccaatagt tatcccccgc taccaggcag
     4741 gttacctacg cgttactcac ccgttcgcaa ctcatccaga gaagcaagct cctccttcag
     4801 cgttctactt gcatgtatta ggcacgccgc cagcgttcgt cctgagccag gatcaaactc
     4861 tcattaaaag tttgagttct cactcatttc tgtcactgac agatttattg ttttttcatt
     4921 gttcagtact acaacttatg ttgtagcgcc ctgcacattg gttcgtcttg ttcagttttc
     4981 aaaggtcttt gtcacttgct tctctcaagc gacaactata ttagtatatc acagccactt
     5041 tcacttgtca acactttttt gaagattttt aagctttttt aaactttttt tcatcaagtg
     5101 ttccaaccgc aacataccat agtccgtacg ggattcgaac ccgtgttacc gccgtgaaaa
     5161 ggcggtgtct taaccccttg accaacggac ctgagttgtt attttcaact cttactatta
     5221 tacagtcttt tcaaactttg tcaactactt tttctaattt ttttcatttt ttttgcatga
     5281 cttactagtg ggtacggata gtaaactcct taaacacaat gcgtaagtct cttaatactc
     5341 tttgacgccc tcgaaatcgt tcattgctta agacgcgttc tagttcttct tgtttctctt
     5401 tactttgttt gtttctataa tctcttagtg tttcatgaaa gagataataa tcatctagcc
     5461 agagaccccc ctcacttata cgatgactga tctcacccac ttcttcatag ggttctttat
     5521 cgtatctacg cttctgactt tcctgcttac ggatatagtc taaaattcga tttcgaaact
     5581 tggttttgaa atatttcctt aaacgtggaa tatcgtctac cagtccttct tctctactaa
     5641 tcaattcatg taagcagagc atgccttctt ggtcccaatc cgacaattcc cataaatgaa
     5701 ggtaatattc atttctacac ttataaacag tcccttggac ttcttcatac aattctttaa
     5761 tcataatttt cccctttcta gatacagtct aacagattag aaaacacatt tgttccattt
     5821 tctccattct gttcccttta gctcctaaac tgcacaaaaa agagagggtc cagcctctct
     5881 cagggttatt tttcgtcatt catttttgac tttacttcat cataggatag tgcatgagat
     5941 tcctcttcta ctgcttcagg catctttcct gtttcgtaaa gagctttaat ttgtgtacta
     6001 tccaatgttt cgtatttcaa taatgcttct gcaattaact tgtgagtttc acgatttgac
     6061 tgaataattt cagcagcttt atttcgtgcc tcatttaata atgaacgaac ctcttcatca
     6121 atttcataag ctgtttgttc tgaaattgat ttttgaggac tctgtgcacc aagcatagca
     6181 tggtttcctt catattgtac tgggccaagt ttttcactca taccgtactc tgtaaccatt
     6241 gcacgtgcca tttgtgtcgc ttgttcaaag tcgtttgaag ctcctgtagt ttggacatta
     6301 aagataattt cttcagctac acgtccaccc attaagccag ccaattgctc tttcatatct
     6361 tctttagata gaagcatttg atcctcttta ggaagtgcaa tcatgtatcc gcctgcacgg
     6421 ccgcgtggta caattgtaac cttatggaca acgcgagcat tcgacaagac tagaccaaca
     6481 atggtatgtc ctgcctcatg gtaagcaacc aattctcgtt ctttttgtga aactgtctta
     6541 tctttcttag aaggtccagc aataactcta tcttctgctt catcaatatc tgaagcatca
     6601 attatcgatt tattgcgacg agcagcaact aaagctgctt cattcaagac attctctaaa
     6661 tcagcaccaa caaagcctgg agtttgttga gccactaatt tcaaatcaac atcttctgct
     6721 aaaggcttgt tcttagcgtg aactttcaag attgcttcac gacctttaac atcaggacgg
     6781 ccaaccaata cttttctatc aaaacgtcct ggacgcaaaa gggcaggatc aagtacatct
     6841 gaacggtttg tcgcagcgat gacgataatc ccttcatttc cctcaaaacc atccatctca
     6901 atcaaaagtt ggttcaaggt ttgttcacgt tcgtcattac ctccgccgag accgactcca
     6961 cgttgacgtc caacagcatc aatttcatcg ataaagatga tagctggtgc tgcttttttg
     7021 gcatcctcaa aaagagagcg aacacgacta gctccaactc cgacaaacat ttctacaaag
     7081 tcagaacctg agatactaaa gaatggaaca cctgcttctc cagcgactgc cttagcaagc
     7141 aaagtcttac ctgtccccgg aggtccctcc aaaagaacac ctgctggaat acgggctcca
     7201 agttttgtga atcgttttgg atcttttaag aactcaacaa cttcaactag ttcttgtttt
     7261 tcttcctcag ctccagcaac atctgaaaat cttactttaa tatcttcttt atttgctgct
     7321 ttagccttac tacgtccaaa actcattgga ttacggccat tgcctcctcc catatttccc
     7381 atcatagaga ataggaagaa gaatagaatt ccaaatggca caatggatac gagtagatta
     7441 atccatatac cacttgaact ttcatgctta acagttactt ctgctttatg gtcagtagca
     7501 agtttttgca attctgatac ggtagtatct gcaggaagaa tagtgctggt aaatttctct
     7561 accttagtaa cagatggcgt gaaaaactga atacctgttc cttctttact tgttttagga
     7621 tttttataga caccagaaac ttcgataaca ctaccatttg gttggtaagt taattctttt
     7681 acattaccat cggtaatttc ttgtaccaac tcagtatagt tgatttgctg acttcctcct
     7741 gagttattcc cagaatagaa atactggaat cctgtcacaa ggaaaaagat aaataataac
     7801 catagaaaag gatttttaat taaaccatta ttttgttttt tcattaaaga ttattctttc
     7861 taatttgaat acacttcctc tttcaatact ccaatataag gaagattacg ataattttct
     7921 ttgtagtcta aaccataacc tactacaaac tcatttggga tagtaaagca ggtatagtct
     7981 gcctcaattt ctacaacacg tccttctggt ttatccaaca aggttgcaat tttaacagaa
     8041 gctgcttctc ttgctttaaa catatctcgc aaattcttca aagtttgacc tgtatcaatg
     8101 atatcttcta caaatagaac atgtcttcct ttgatatctt gagtcacatc ttgtttaata
     8161 ttgataacac cactacttgc tgttccacca tggtagctag aaaccatcat gaagtccatt
     8221 tcaatatgtg tatcaatatg tttgaccaat tcagccataa aaggaataga tccttttaaa
     8281 atcccaacta agattggatt ttttcctgca taatctttag ttaattgagc acctagtttt
     8341 ttagctgctt ctgtaatttc atcgtgtgaa acgaggactt ttttaatatc gttttctaac
     8401 attttttacc tatctatttt ttctatataa agtacagtgt tcattatatc atttttcgtt
     8461 tttttactca aattattggt cgcaattccc aaaattgaga caatttcacc aaattgctca
     8521 ataataagag cagagtttct cttttccata gggattttca aatcaataaa taaacgtctg
     8581 agtttttttc tatgcccatt ttgaatcaaa acatctcctg tttttcgatg acgaatgtgt
     8641 atggatgttt cacgtgaaac aggtatttgt tgaattgatt caccttctaa tggaagtcca
     8701 aaggaaaata aatatccttg ataagctacc tgattttgat agtgtaacac aagttcatct
     8761 tccttttcat cagcctgcgg actgatttta caaatctgaa actgttggta ctcttttatc
     8821 aattcatagc catttttaat cggatgacga tactggcttt tagattttaa aatctgctga
     8881 acttcagcaa actgagcttt tgtaagattc aaatctggaa aacgattcag ataagtttga
     8941 agtaaaactc tttgtgtaga ctcagagtaa gaaaataact gctgtaaatc ttccacatta
     9001 atattgttag ataattcagc tattgccaaa tcataatcta aaatttcatt gccaatgcct
     9061 aagattgcat ccctaaatcg aggattttct ttttccaatt ctggtaagta agagtttcga
     9121 atacgatttc gaaaataatg attctcctga tttgatgtat cttcaaagtg aaaaattgat
     9181 ggaaagtctt ttttctgaaa atgcaagaag ggacgaatga tttctatctc tccgactact
     9241 tgcttctcct taattcctga tagatagcgc aagcgagttc ctcgaatcaa gcgcatcaaa
     9301 atcgtttcca cctgatcatc agcatggtgg gcagtgacta aagctgtcgc acctgtcttt
     9361 ttcataacct cttgaaaaaa atcataacga aaatttcgtg cacgcgcttc tgaaaattct
     9421 cctgaaaaat tgctgatata aataggaagc tctgcttcag cagccaactt ccttaattcc
     9481 ttttcttccc aatctgattc aattctctgc ttatgattca catgagccag aatcaattca
     9541 atctctaact ctttttgata agtagacaat accttaaata gaaacataga atctaatcca
     9601 ccagaaagag ctagaaccgc cttagcatgc tttttgaaat atcccttctt gagaaaatga
     9661 tttaaaaaat ctggttccct cattttagaa cctcataaac atccttggct atcttagaaa
     9721 tcgtatcata atcagaattc ttagtgaaaa tagaaagaat aaatggagaa tctgcataga
     9781 caacacccgt atcatgctta aattcatccg catctccaat tttatgagct actttaacag
     9841 aaacaccttt ggcaattcgc tgactatcaa aatctgtttt agtcaaagac tctagcacaa
     9901 atccattttg attataaata gcttccataa acttcccggc catcttagaa gaaatcaatt
     9961 tttcttttgg atcccaatca tctcccataa tggcagacat tttggatttg aatgtggcat
    10021 cagattggtt tgaaatgtaa tatcccaata gattatgagc tacattatca gattcttttg
    10081 atactttcgt aattaaatcc tttaaagaat attctttatt atcttctttt ttaggaagac
    10141 taccacttcc ctctggttta taagaacctg gaaaatcatt gactgcagat acgtatttta
    10201 cagtcgtatc taactgataa agaccctcat ttattttttc ttgcgtataa tagagatagg
    10261 agagtttcaa aacgctggct gcatacatct tttcatcttg attgatacca gcttcttttc
    10321 cagtagtcag ttgcttaaca taaatagaga aagaatcttt ctgatatttt tcagataaca
    10381 tttcttgaac tttactcatc cgattatctt cttcagaagt tgattcttta gctacccatc
    10441 cagcctgatc aatatgtaga aattctcttc cttctacaaa catggtcttg tcgattgata
    10501 cttgcgaata agctgataag gatgatttca cttcttttaa atcataagga ctattgtaca
    10561 gtttaaagtc agattctaac catacttttt ttattgttgg agttacctct gattgatcat
    10621 ataaaaatcg tttgtccgca gctataaatt gatgatttga tagcttaaat actggaatcc
    10681 cttgtttatt taagcgccac tcggttattt gaaaacttgt tttgggagtc aattccccag
    10741 attccactac taaatcttca ttcgcataaa caggaacctc tccataaacc atgggagaac
    10801 ttaatttttc tctaaaataa ataccaaagt cagattgtga aaggtaataa atttctttcg
    10861 aagtatagac gacttctttt tctgtgctaa cgacttttga aatggtcaaa aaacttggta
    10921 gcaacaaaat aattaagaat ttacgcattc ttcctttcct tttcttcttg taaacgcaat
    10981 aattgatttt tatcagccaa ttcttccaac ttttcatctg acaaacccaa aaattgctct
    11041 attacgtcta ataaattttc cattttatca cctttgaagc aagtcaggaa tcgtataaac
    11101 tttttccctc gacttagaat aatagtactt cgctcgtgta tatttagcag cataatcttc
    11161 atctttcaac ttggtagcaa atgctgtctc cttatccttt tcatcactca aagtttgata
    11221 ctgagtttgc aagtctgcta attgctgacg tctttggagt aattgctgat aactctgcgc
    11281 taaattaaaa gttggcaaga taaataatag cataatcaaa atcaataccc ctcccataaa
    11341 acgattccgt ttttgtcgtt ctttcatcag gtagcgacga cgttggtatt cattttgaat
    11401 aaaagaatta ttcaattgta caatattttt agacattttc ttctacccgt gtttcactga
    11461 taatttcata cattcctgct gcatcttctt tttttgtact atctttcatc tctagtactt
    11521 ttacaagcag caacttattg ccaaagcgaa tttcaacttg gtcattaact ttcaagtccg
    11581 ttgaactttt ggccaagatt ccattaacct tgattctacc tttatctgct acttcctttg
    11641 cgactgtacg acgcttgata attcgcgata cttttaaata tttatctaat ctcatcctta
    11701 ttacctcaat ttattattgt accattttta tctattttat agaagaaaaa tatcaaatgg
    11761 aattttcttc cttagactct tttatctcta ataaactttc tccaaaaatc agcagacctt
    11821 ctaaaatttc ataatctttc ttattttgaa catcaaatac aagctccatt aattccttat
    11881 tctcagcgat gcctgctttt aagttcgtta cggataaagc tttaaaataa tcttgagcta
    11941 aaaacagtcg ttgagtgact ttttcaaatt gaattgtaat tttattatct tttctttcca
    12001 cacgttgaac aaagaccttg tccaagtatg atttgaccaa accaatctct aaaagatagg
    12061 ctactacatc tgggtattct ccaaaacggt ctatcaactc ctcttgtaac tcttcataat
    12121 tgacacggtt gtcaatttga cgaattttct tgtaaatttc aatcttatgt cgttgatcag
    12181 aaatataagt atcaggaaga taggcatcaa tttgcaaaat caactcagca ttccctttgg
    12241 ttcttgtgtt agcgttagca ttaccgtttc gtttagcaat agcttcctct aataactgcg
    12301 aatacaattc aaaaccaaca gaatcaatga aaccagactg ggattttcct aaaagatttc
    12361 ctgctccacg aatcgaaaga tctcgcattg caatcttaaa gccagagccc aattctgtaa
    12421 atcctttaat cgcttctaat ctcttttcag agacttcact gattgatttt tctggacgat
    12481 acatgagata agcataagca atacgattac tacgaccgac tcttcctctt aactgatata
    12541 aggttgacaa gcccatatgg tccgcatttt caataaataa agtattagca tttggaatgt
    12601 ccacccctgt ctcaataata gtagtcgtca ccaagatatc gtattgtccc tcaataaagt
    12661 ctaatagagt attttccaac tggacttcac tcattcgacc atgaacatat ccaatcgaag
    12721 cctccggaat caactcctgt aattctgaaa ccttctgaac aattgtgtca actttgttgt
    12781 aaagataata aacttgacct ccacgctcca tttcacgcaa gacagcatca cgaatgacac
    12841 tatcattctt ttccaaaaca taggtctgaa caggatagcg attagtcggc ggagtttcaa
    12901 taacagataa atctctgatt cccagcatag acatatggag ggtacgaggg attggcgtag
    12961 cggtcaaggt taggacatcc acttgtttct tcagttcttt caaagtttcc ttatgcttga
    13021 caccaaatcg ctgttcctca tcaataatca tcaagcccaa atcagcaaac acaacatctt
    13081 ttgacaaaac acgatgtgtt ccaatcaaaa tatcgacttg accgtttttc aatttttcaa
    13141 gtgttgcagt ctgctctttt ttacttctaa agcgactcaa cacatcaata ttaactgcaa
    13201 aattttggaa tcgttcctta aaattcgtat agtgctgttg cgctaaaacc gtcgtcggaa
    13261 ctagaatgac aacctgtttg tgatcattga ctgctttaaa ggctgcacgc atagcaactt
    13321 cagtctttcc aaaaccaaca tccccaacta aaagtcgatc cattggctga gaagcctgca
    13381 tatccctctt gatttcctca atactacgaa gttgatcatc cgtttcaaca taagggaaag
    13441 catcatcaaa ggcatcttga tcatcatcat cagctgagaa agcaaaaccc ttcaactgac
    13501 tacgttcaga gtagagtttg attaaatcat cagctatatc ctctacctgg ttcttaacct
    13561 tttgcttggc ctttttaaaa tgaccgtcat ttaatttatt gagttttgga gctttaccat
    13621 cacttgaaat atatttggac agtagatgaa tctgttccac gggaatagaa atttgatcac
    13681 cattttggta ttggacactg acataatcac gatgaattcc cttgatttca atggtttcaa
    13741 ttcctagata ttgaccaatc ccatggatat gatggacaac atagtcccct ttttcaagtt
    13801 cattgtaatc ttttaatctc tctgcatttg aaacatgttg tcttcgaaaa cgacgcttta
    13861 atttcttttg aaaaatctca tgttcagtta tcaataaaat cttttcatct acaaaatgaa
    13921 aaccatgtct gagattaccc tctattaagt ttacagattc tttacagata tttgtcttat
    13981 ctctagaatc caatttaatc tgatattcct ctaacatatc ctccaatgtt ttacttccca
    14041 ttgaattgct agactgcaga ataatggtgt aatccatttt tttatatcgt tcaatttctt
    14101 cttttagaaa agaaaactga ttgaaaaatt cctgcatagg atattgattg aattgataaa
    14161 ttttgtcaaa tttgagattt cctaaaccct tttgaagatt agaaaaaaag gtcactggac
    14221 tttgtttttt atagatttgt tcaatatcag aaaaatactg catatcagaa aatgctttac
    14281 tattctgtaa ttcttctgta aagtactgcg ctaattctct ttcaaagact tcatactgat
    14341 tcatcaattt ttgataatca tcaaagaata ttggagtatc tttttcaata taatcaaaga
    14401 cagtccatgt cttatcatag cacaaagata aaaacttccg agagtctgca tgactttgtt
    14461 tttggtgaaa acttgaaaga atttcttcta ggtatgattt caaaataggt gataaagttt
    14521 ttgaaatttg tttttctaaa gctgactgtc ctcgttgata atccttttct ctcaaaagca
    14581 tatcactagc tggaaagata gtgagttctg tcttattttc tttcgataat tgtgtttcta
    14641 cttcaaatga cctgatacca tcaatttcat caccaaaaaa ctcaattcga caaggttcta
    14701 actgggatat ttcaaaaata tctaaaatat ctcctcgaag actaaattcg ccctgagttt
    14761 gtacttgagt aacttttcga tagccatttt cctttaactg atggataaac gcgtgttgat
    14821 catattcttc accaactgag atttttacaa tactatcttt gaatgcattg ggagacggta
    14881 aaatcaatcg acttgctgcg atattacaaa ctaaaatccc tttcttagat gaatcagtca
    14941 aaaaacgcaa ggcttcaacc cgtgaaataa ttttttcctg tgaagacatc aaaaactcca
    15001 ccataggagc atcatctacc aaaaatggat agacgagttc ctcacccaag atagatataa
    15061 gatcactaac aagtccttct gcttctccat aagttgacat caataacaca atcctatctt
    15121 ctttttctaa actgcttgca attgcaagag ccttagtaga tgttgataaa ccaagtatta
    15181 gttgtctttt cttatctgtt aaattttgat gccatttttt aatctgatca ttttctgaga
    15241 ataaatctaa taaggtcacc atttatccgt tatacctctg cattgttttc tcaaaatttt
    15301 tctcttgtaa atagtagttt acagaatcgt caactttgtc aacagactgt aaaataccga
    15361 tatactcatc cctgtcaaac ttactcaaaa catgatgaac aactgacata ccatttttag
    15421 gtcttccaat tccaatctta acacggttaa agacctgagt tcctatatgt tgaataatag
    15481 acttgatacc attatgacca cctgctgagc cttttgctct taaacgaatt ttcccaactt
    15541 ccatgtcaag atcatcgtaa atgataagta aatcgtcaat atccaaacca tagtaagtta
    15601 ataaagcatg aactgctttt ccactttcat tcataaaggt cgttggttta accagataaa
    15661 ttttttctcc atttaggaaa aaggatgcta ggtcagcttg aaatatctta tcgtgtgtaa
    15721 aagtgacatt ctgtttcttc gctagttgat caatcaacat aaaaccaaca ttgtgttttg
    15781 tttcaaaata tttatcccct ggatttccca agcctacaag taatttggtc atttattttt
    15841 cctttcaaaa gccaaaaggg ttggaatttt tttccaacct aattgacacc atttattaat
    15901 ttttagacat taaagcggaa ttccatgata tcgccatctt gaacgatata ttcttttcct
    15961 tcttcacgca agcgtccagc ttcttttacg gccttttcag atccgtattt cactagatct
    16021 tcatatgaca tggttactgc acgaataaag cctttttcaa agtctgagtg gataatacca
    16081 gctgcttgag gagccttcat accacgtttg aaagtccaag cgcgaacttc tttttcacca
    16141 gctgtaaagt aagttcccaa tccaagcaag tggtaagctg cacgcgtcaa cttatctaca
    16201 cctgattctg tcaaaccaat ggcttcaaga aactcttttt tatcttcatc atccaattca
    16261 gaaatttctt cctcagcacg cgcagaaata acgactactt cagcattttc tgtcgctgca
    16321 aattcacgaa tttgtttgac atagtcgata gagtcaggtt ctgaaaccac atcctcgtcc
    16381 acattagcta cataaagaac tggtttagtc gtcaaaagga aaagaccttt gacaaccttt
    16441 tgttcctcat ctgtaaattc aatggtacga gctgatttcc cgtcttctag gactggttta
    16501 atcttttgaa gaacattaaa ttctgctact gattctttat ctttttgcgt acgtgccatc
    16561 ttttctacac gcgcatatcg tttgttcact gattctaagt cagcaagaat taattccaga
    16621 ttaattgtat caatatctgc aagtggatct acaaaggcgt cttcacgtcc ttgctcgcgc
    16681 atcacatttt catcatcaaa agcacgaact acgtgaacaa tcgcatctac ttcacgaata
    16741 ttggccaaga atttattccc tagcccttct ccttttgaag ctccttttac aatccctgca
    16801 atatccgtaa attcaaatgt tgtgggaact gtctttttag gagttatcat ttcagttagt
    16861 ttttgtaggc gttcatctgg aacttccacc attccaacat ttggatcaat agtcgcaaat
    16921 gggtagtttg ctgcctctgc tcctgctttt gtaattgcat taaatagtgt tgatttacca
    16981 acgtttggca aaccaacgat acctgctgtc aaagccatat ttctcattct ccgttttcat
    17041 ttcaatccct aatattataa cacaaaaagg gaaaacttgc taaaattcct tgatatagct
    17101 atttcccata actgaaataa taattttatg gactttttta ggaacttttt aatttttcaa
    17161 tggctttttc ataaaatgaa gtagcatttt tctgattgtc tttggatagg tggctataaa
    17221 tatccatggt catagaaagt tttgcatgac cgagccgtgt ttgtatctct ttgtaaggta
    17281 gtccagcatt gagcaagata ctagcatgag tgtggcggaa ggcgtggaac gtaaaacgag
    17341 ggcatccagc acgttccaaa tgttttacta acctgaaact aagcattcta gcgtctatat
    17401 gcttatcaaa gctattagag aatactttct cataggtcaa ccccatttct ttccctattt
    17461 gggattgtct tgctttatag agccgtagca tgagcaccgt cttattatcc aagtcaatta
    17521 ccctaatact tgatttagtc ttagggacag tgatttcttt gataatattt aacgttttat
    17581 ttacgtctag tgtgccgttt tttaggtcaa tatcagacca tttgagggct aaacactcac
    17641 gaatacgcaa accagtcgct aaaagcgtct tatatagcac cgtatcgtag aaatttttgt
    17701 aagtgtttgg cagctgctcc aggtaagtca agaatttttt taagttatca tcatccagat
    17761 atttcagttt ttgcccttct ttttccttac gacgtggcac gataacatca cgggcagggt
    17821 taaaggggat agcttgcaaa gatacggcat atttaagtat gcgtttgttt agagagtgaa
    17881 ggagcggata ttcttggaat cctttgccta gttgattata gtcttttgcc cattgattga
    17941 cttgcttttg aatgattggg ggcgtcagtt tatccagcct ataatttccg aaagctggaa
    18001 gtagatattt atctattttg aatttggtac ccattaaggt cgatttttta accgtatggc
    18061 agtacgtttc tagccaattc tccaccagtt cggcgtaagt ctttatctca actgctttat
    18121 atactgtagc ccctcctttt tcaaagtcta tttgagcctg tagtgctttg gctttgagtt
    18181 ctctcttggt tcgccctgat atagtcgtct tgactttctt acctgttacg gtatcgatac
    18241 caaggtagac gctagagcga tagactgtag aaccgtcttt tttggtgtat tctgttattt
    18301 tcatggtttt actccttttt ccatcagcag gcaagcaatt agaaaaggtt ttgagtttat
    18361 accatgcgag ggactgcgag aatgccccta ttttcgattt taattagcca ggcggtaaat
    18421 aataccagaa taggaaataa ggcggatatg ggatttgtat gggcgttttt acatatcatc
    18481 aaagagtgaa agttggttat tatccggttg ctttttaagt ttcttttgtt tgttttttag
    18541 gtcttggcaa ccacactttt ttcggagtct tcacataaat gttggaaaaa gtcggatttt
    18601 caagaatact ggaaaaaaac aattttcttc attttctgag tccctaaaaa gcgatggggt
    18661 aaacaattcc aatcccgttt ctattagttc ttaatggttg gaaaactaac atttctaaca
    18721 ccctaaaaag gagaagggtt tagacaaggt cagaggactt gctgaagccc gagaaagcac
    18781 gcgcttcagg attgttgaac attgtggcgc gtggtaggct ataggtcgca ttttcggcac
    18841 tttcggcagc cctaaaagaa aaatagtagc gatgcgcttc atgtttgttg caattttgcc
    18901 caattttacg ctttagtctg taaatcggct ttctattctt caattttccc cgtttttgca
    18961 caatagacca tgaaaaaagg gggtattcga tgaggtggta tatttttgtt tatgaattat
    19021 gggtaataac atctaaagct ccaataatct tttgagcatt ttcaatagct tctttgtatt
    19081 ctttagatgt gtttttaaca ggttttttaa ttaaatctat aaaaacaact ggtttagtaa
    19141 aatcgttaga tgttattcgg attgtcatat tcaaaatctt ggtagttgct tttcttttag
    19201 cgacgattcc gccagctacg gctcctatta caccaaatac cgccccagct actaaagctt
    19261 gtccaacacc gccggaaaca acagactgat cgtttataac taattcgtaa gatactaaat
    19321 catcgaagga gtaccagtcg gtatcatttt tatctttttt aattaaagat ggaatcaaag
    19381 acaaaccgcc tgtcccaagc gctaaggtag tttttccaac aactttagca gttccgccaa
    19441 tcagacctga tgaacctttt gcttttcttg ctccgtgtat tctatatgta cgatgatttt
    19501 tgtctatttc aagaggtccg actttatcag tacgtttact tcttacagta gaaggtctag
    19561 acgatggagt tgctactttt tggggagttg ttttaagcga ggcattgatt gagtatccgc
    19621 aattcggaca aaacttgcaa ccgacaacag aatttccgca ttcaggacaa aatttcataa
    19681 tttttttact ccttttttac ttttctctat aaatgcccac gacttcgccg atggtgcgta
    19741 tatcgttgct ttcgtctagt tgaatatcag cacattcagg gttaaggctt tctaaatacc
    19801 cttgacgcag tttcttgacg tagttagcgc cgtctacttg gaagataccg atagtgttat
    19861 agtcaacttg aggtgtattc ttgataaata ggtagtcgcc attctttatc tttggctcca
    19921 tggagttgcc gacgacataa gcgatagcgt catagtcgtc tgggatttca tcagtataaa
    19981 agcatttttc tatgtttagg ttatcgtctt gggttatccc gtttccggcg gttacaactc
    20041 cgataacagg gagcgtcttg ctttctctgt agtcgtccag actgataatg ttttctgata
    20101 ctatgttttc gtttttctgc tccagcagct gctttttaag ataagtcaag gctttttctt
    20161 gtcttggtgg agtgagttgg tcatagatgg attggatggg ggaagtggta ggtgtttctg
    20221 cgtttttatt ttctactaag tcagactttg aaacgttgaa aaaattagcc agcatttcga
    20281 ttttatctat acgtgggtaa gttttagcat ttacccaatc gttgacggtt gtatacttaa
    20341 aatctaatgc aatagctaat tgacgagggt ttaaaccttt tgcacttaaa agtcttttga
    20401 tattgtccgc cattatttgc ttatttccta atgacatggt gcaaccgcct ttctataatt
    20461 cataatttaa ttatacggtt aaaacgtaga aaaagcaagg gaaacaaaaa aaaagaaaat
    20521 aaaatcaaaa aaagtgttga caagttatcg gttttaccgt atactgtcat tgtatccgat
    20581 ttaaacggat atccaaaaga aaggagtgag ggctatttgc gtcttacatt gaaagcgttg
    20641 cgggctaata gcaacatgaa acaatcagag gtagcacaaa aattaggaat ttctacaact
    20701 acttggagta agtgggaaaa tggaaaaagt tttcctgatg ttgcccaagt aaaagagatt
    20761 gaaaaacttt tcggtgttgc ttatgacgac attatttttt taagctaata tccgatttaa
    20821 acggatatag aaaggagcga cccaatcgca atactatatt acatctacaa gatactccac
    20881 tggtgcttta ccactgggga ttgaccttct cggaagaaat atccgaccta gaacataaag
    20941 gagaaaacaa catgaacaat aaacaagtat atcaagcaca aacaactgac cacggtatcg
    21001 ctagagcatt aggcgaagaa attcgtatga tcacagacgt tgaagaaaca acttacaatg
    21061 gacacggtga aatcattgtt gagtacacca gtgacgttgc attgacaaaa ctatttgacc
    21121 gtattaaagc actttacgaa aaaggcgaaa gctacgaaac aaaaccattt ttacatttta
    21181 aagtattgga agatgatatt gaaccaagcc caaatggagt ttttactgct aaagaggtgg
    21241 cagagttttt aggaatttga ttaacaataa ggagcaaacc aatggaacta gtctacatgg
    21301 acggcaagaa agagccgtat acactgagca gtattgtggc agagtgtgca gaagttaagc
    21361 acagacattt aaagattttg ctgaataagc acagagagga ctttgagagc ttcggaaagg
    21421 tgcaatttaa aatttcacct tcagagagtg ggcaaaatgt acgagattat attttgaacg
    21481 agcaacaagc tactttgtta atcacttact tacgaaatac agaaccagtt cgtaaattca
    21541 aaatgaacct ggtcaaagct ttctttgaaa tgcgtgagga gctttctaag tttcgtatgc
    21601 agagggcgct agaaaagcca aaaagaaaaa ccttgcatga cagtattgag aactgggaac
    21661 aagcacccaa gcacgcgcat agcactatga acaacctgct actaaaagca gtaaccgaca
    21721 ggaacgctaa gcagttaagg gaagaacgtg gaggctataa tggcatcgat agcttgacca
    21781 gtgacgagct ggagcaatat caagcctttg aagacatggt aatagccatg attggcttga
    21841 atatgagtta tcaggaaatc aaggctatgg cattcagaaa taaaaaaaca cgccaaagaa
    21901 gcgcgtgatt gcaacaaaaa aggcttagca ataaccacac cgcaaagcct ttcacactaa
    21961 gactaaaacg aatttaacaa aaagcaggca agctattatt aaagaggttt tagtaagatt
    22021 tatacctaga ttatagcata atacagcggt ttccgctagt acattgaaaa taaaataagg
    22081 tacgctggga agattttaat ttttttcggt ttaggggttg acatttaaga cgacaagaat
    22141 tataataatt tatgtcgtca agaaaggagg caatccgatg actgacaaaa taaagaaagt
    22201 aggacgtccg aagggagaaa actctaatac cgtaaaatta acagtcagag ttagcgaaca
    22261 gctaaatagt aagctggaag agtacggtaa aaagaatggt ttgacaaaac ctgaaacctt
    22321 gagaaaagcc tttgaaaatc ttactaaata aaaaatacct tgcaacttgt caaacgccaa
    22381 tccgagaagt tgcaaagtat ccgcccccac gaatgaggta cgtaaatatc ttatcatgcg
    22441 tactcttatt tgtcaaacat agaaatagag tgcgcttttt gcgtacccaa aaatgaggta
    22501 aagaatggga aaacaagtat ttacacaaga aatactaaga aacatacaag aagaaaatgg
    22561 aatcatcaca gtagacttaa tactagacgc tttaccaact tggtcagaaa aagccatcaa
    22621 gggacgttta tcaaattggc gttaccgtaa agttattgac tacagggtag aagatgggga
    22681 atttagtgag attttcctac taagaagcaa gcaggagata aaagaagagg tcagtgccgg
    22741 tcaacgtttg aaaatggatt tatattttag acaggtttta gccttaactg gaatcattga
    22801 atcaaacacc agcaaggaca acgacaagac caaggcgatt gaattgcaac agaaagcaat
    22861 gagagcaata ccagacgaca tttacaaaga gttgtcagag atttatgagt aaataacaaa
    22921 actaagaaaa actaagaaaa ccgaagtgca ggcaagcaat tagaaaaggt tttgaaaagc
    22981 atagagcgcc aactctttaa actggtactt tctcacgctt tcaatttggc gagtctgagc
    23041 gtgaggctag tacttacaag aaaaaaactg tataagtaaa aagccccacg ctcagaagtt
    23101 tggcgaccga gagcgtgagg catgtggcaa gaaaaactgt ataagaaaca accattcaaa
    23161 atccccacac tcgccatcgc tcaactttga gtgtgaggat atcctggata gtaaaaggca
    23221 ttaaaaagcc cttagggcaa ctacacaaca tagaaacaga ggtacaagta tggaagaaac
    23281 aacatacgat attatcgcta aaagcttgga tagaattagt atggaattac accaagctga
    23341 cgaaaataac gattttctag aatatgggtt ttatcaggac aattaaaagg ccaacggagg
    23401 aaaatatgat agcagatcaa ttaaaagcta tttcaacaaa tttagaagaa atacaagaaa
    23461 ttcttgtagg agatggaact gcattagcta aaagagaaat cagcagggta ttgaacgact
    23521 tggacgatgt ttataatgaa ctaactagca cagaataccg tgaacaacag gaaacactta
    23581 aggaacagac ggaaaggatg aaacagagag tagttgctga agtaatagga ggactagaag
    23641 aacgagaaga gtcttattac agaaattatt ggggtgatac taagtttata aagctacttt
    23701 ccaactttga tggatttctt ttcttaaaca cttacctagc tgaaataact aaagaaaata
    23761 cctacccaat ggaacaaagt caattattga attatgtttg ggaagtcata gcggttgata
    23821 tcgcaaagaa aaaacgagga aaaaagaacg ttttggattc atggacagat tcatccttat
    23881 atactcgtgg aatgatgatt gattaggagg caatgcaatg acactagacc taggcaaaat
    23941 gacacaagca gaatttgatg aagtaatggc tgacatcaag gcaagaaatc caaacctatt
    24001 acagttcatc actgattttt tagatagaaa ggtaacaccc ggagaggtgg acgactagtt
    24061 gaatatggag cgagctgaac aagtggaata tattaagaat tatcaagcga gggcataaca
    24121 tgaatgaact agatataagt aacacacagg ctgctgtctt aatcgtgatt ttgattggtt
    24181 tactgcttta tttaaaccac cgagaccgca aaaaaagcgc ccaaattgag cgagaaaacc
    24241 aacagacgat aaaaacacct agcgaggatt taaaccctga atatgggcga tatatccagc
    24301 ttgcaggagt caacgtatgg ggagggatgg aatgagttac atagtgaaga tataccttga
    24361 tagtgaaaaa ataccagatg aaccttattt tactaaagag atttttctag ctacgtgtat
    24421 gggcaatgca acaaagttag ctgtaactat gggcttgttg acaaaacaag tgttggattt
    24481 agaaactaag acagctatct tgaaagccct tgatacaatc atagagactc aaaaagctat
    24541 tttgcaaggg gtagcgagcg gacaaataac ctttgtaaat aaaagtaaaa gagaggagga
    24601 actggatgag cctatcagag aacgataaac gagtattaag attaatcaag gtaggggctg
    24661 agaactccat aacagggtca gaaattagcc tgacaaccaa gctaacagaa agaactgtac
    24721 gtgacattat caagcgtcta gtagttaagc acaacatccc tattgtaggg gttagatgtg
    24781 gcgtttttag tgggtacttt attccagcga ataaaggcga gttactagat ggcgctaagg
    24841 ctttttacaa ccaagtacag gaggagagca agcgcctagc ggtgttgatg aatagcgacc
    24901 tagaaagcta caaagaaacc ttgaaggagg tgggcggata tgtttagcct aagccgagaa
    24961 agcgagcaag acctagcgca tggcattctg gaggtagtgg aaagatacct ggaagcgcgt
    25021 gagaaagtac cgccaagatt gaccaagtta ataaatagta gttgaactca aagaggaatt
    25081 gaaaattagt gacaacacgc tgaataagtg ggaaagtcag ggtttaagac gctatcaacc
    25141 gccggacgat ggttcaagaa tgatttacta tttagtaact gacatctgga agtttctggg
    25201 ggtggatgaa tgacaattta cgaggctaga ggtttccaga gtaatcttgt ctatccattt
    25261 gataagattg agccgttcca gtacattgaa cgctttaagc ctttggtagt ccctgaaagt
    25321 gctgatacgg aggaatacaa aagaacccag gcgccatatt gtttaagtgg taaggttatg
    25381 ccggagaaaa acgggagtta caaacgaaac aactccagtc taatctatcg ggatttgatt
    25441 ttccttgatt atgacgatat acaggggacg actgaggact tcatagaagc cgtttctagc
    25501 gctttgtttg gctactctta tatcttatac ctgactatca aacatagcat agaaaagccc
    25561 cgttttcgcc ttgtggtgaa atctaacaac gtgatgaacg aggcaaccta taagcaggta
    25621 gtaaaagaga ttgctgacaa gattgggcta ccctttgaca tggccagttt aacctggtca
    25681 caactccaag ggctaccagt aacaacaggc gacccagcaa cttatcaaaa gattgtagag
    25741 catggacttg attatccagt ccccaaggtt gaaccaagag cgaagcaagg aactactgag
    25801 cgttataaac ctagagtaag tggccagagg tcaatgacta tgaggattat tgacacgctt
    25861 ttcaacggat ttggagacga aggagggcgc aacgtagcgc tgacacgctt tgtgggatta
    25921 ctcttcaata agtgggttga ttgtgaccta gagacagctt atgaactgac aaagatagcc
    25981 aatagcgtga cggttgaacc attgcctatt gaggagctag accgtacttt tagcagtatt
    26041 gcacgggctg aatatagaaa gagaggttag aaccatagaa ctagaagaat tagcaaacct
    26101 aaaaagtgaa atcttggagg tcagagagca agaacaacct cctagaacga tgtgggaact
    26161 ttatagtagg atttgggagc ttggggatca atggcgtaag gaaaactcct atatcgtcaa
    26221 tgaaggaaag aaaaacgaaa gggtagaaat cccacgacca agtgttgcaa tcgtagccaa
    26281 agcgttacag gaaatttgcc attttacctt tataggtgag ggggttataa gtgatattag
    26341 taagttatat ctttaccatt tggatttagg ccattatgta tcaagtaacg acattttccg
    26401 aaaattgctt ttaaaatatg actcacgttt gacttctaac aagttctttt tagaacttat
    26461 ctcctatatc cgtacagaaa ctaagatgaa accaccgctg gacgattatc gatatattcc
    26521 agtagctaat ggagtttata atattaaaac tcataaacta gaggaattta gtcctaattt
    26581 tgtcattacc agtaagatac aaacggaata caaaccatgc gccagaaagc ctattttaga
    26641 tggttggttt gattttgata gatggttaga ggctttagca gttaacgata aagaagttgt
    26701 agctttattg tggcaagtca taaatgaggc tatcaatccc aaccgaacac gtaagaaaat
    26761 ggtgctaatg gttggagatg gtaataatgg aaagggtact ttccaatcct tgctggaaaa
    26821 tttaatagga cggtcaaata ttagtaattt gaaacctgac cagtttggga aagagttcta
    26881 tttaggcgct ttggaaggaa aggtttgtaa tattggagat gatatttcta acaagtacct
    26941 ggatgaagtt tctgacctta tgagtgtaat tagtggagat ccagtgcagg tcaataaaaa
    27001 aagcttgcaa cccgtggaag cacgtttcag actattgtgt atcttttcgg gtaatgattt
    27061 gcctagagct agaaataaga ccatggggtg gtatagacgg ctttgtatca ttcctttccg
    27121 tgcagatttt aacggtcaga aagaacgtca tgaaatcaaa gaccgattta taaagaacaa
    27181 agagctgcta gagtgggttt tgtttaaagt tttaaatatg ccagattttg atagctttat
    27241 agaacctgaa gcggtacaaa aaatgcttag caagtacaaa aacgataacg attatatcaa
    27301 agtttgggta gagaatttct acataccaaa cgggtggcat gaaataaacc atgttccaat
    27361 gtttattgca agaaataaat taaaagagtt tgctgaggat ataggtatag ataaacctaa
    27421 acttggacaa tttagcaatg cagtaatggc cgaattggaa aaaacaacgt catataagta
    27481 ctcagccaaa aacggaacgg ttagctctaa gttttatgac gtacttgacc cacaaggttt
    27541 tcaacgtaaa agatttgtta aaggtatatg gggaatacac ttagaagatg aagatttagg
    27601 ttaggaaatt taatactaaa aaaccaatta caccaagggt tatagcaatt taggttatgc
    27661 aaaaaataga aacataacct aaacactaac ctttgtaaac gttgatataa aagtctttgc
    27721 gagtatttag gttagtaggt tagtttttat ttctaatatc tctatataaa aaataatata
    27781 tatacgtgtt atataggcgc gaacttaaaa agtaaaacta accctaatct aacacccttg
    27841 aaacgttgat atagtaggct tttgaagggt tattttttaa aactaatcaa aacctaatct
    27901 aagagaataa aggagcaaaa cagatggcga tgaacatttt gtcaaagagt ggctaaccgt
    27961 tgagcggatc agtgaggcgc tggatggctt tattgagaat gcgaaccata acgctatagt
    28021 aagcaataaa aaacatcgaa cggattcagg aaggttttga aatagtcttt aaagagcttg
    28081 atgaactaaa agcagtacct gacaaacact aaaacattta aacaaaatgg ggtagatagt
    28141 accgaccccc ttgcaatggt gtaactacta gtgacaccct aaaaacagaa agaggaaaaa
    28201 caacatgaca ttaaaaacat tttcagataa agcaaaaaca tttaatttca cttacgaatt
    28261 taaagaccaa gacaccgccc aagtggcagg gagtgcattg atgggctaca tgattggaac
    28321 ttatgtagta ccgtctattt caatcactta caagaataaa gggacgctag tagctgagta
    28381 cgtggaggat cacaagctaa acaagacctt caaacgtatc tgtgacggtt tcaaagacta
    28441 ttacaagcaa ccagagggag atgaagcatt tgaagaacgt tataaacggg agcgcgtgct
    28501 tcaactcaaa gagtcagagg actttgagag cttgctgaat aaggtcacag actacgagct
    28561 gaaactatta gactatgctg aacgcttgct ttctgataag cctatcctta tgaactctat
    28621 gactgctttt ggtacgttgg aagtgctggg taatgaaagt attaacctat tccaaaaatt
    28681 ggacgtagag ggcgaatata aaggccttgc tgattattct ggtcaataaa cacaggggag
    28741 caatcccctt tttttacata aatcagacag aattacacac gaggtagggg gatggttaga
    28801 agtttaaaag caagactaga aaagttagaa gagtatgctg gaaagacgga atatgcaaca
    28861 actgatgaat gtatatcttt atatgtggat tgtttaagat ttgcttttca gcaagatgaa
    28921 ataaagatag atatggatga ttttattaaa acaccattta aagatattaa gctagcagac
    28981 tataagaaat caaaaaaacg tatggatgaa cttagggaaa tgactgggat aagtgagttt
    29041 tcagaattag agcaataaag cgaggtgaac agtatttgac agaattaagc agaaaaaaag
    29101 aaatatttat ggccaattat ctcatgactg ggaatgtaaa aaaagctagt gaattaagtg
    29161 agatcaccag aaagacggct tacaattact tgaacgatgc aacctttaag agaatttacc
    29221 gtgaaagacg tagcgaacaa cttaaggagt ctactacgct gttacagaat gcaagcgttg
    29281 aggcagtcaa tgtattaagg gaaatcatgc ttgataagac agtgagcccg tatgcgagac
    29341 aacaaagcgc ccaaagcatt ctaaacatgg cttataaggc ggttgaaatg gttgaggtag
    29401 tggagcaatt agaaatatta gaggcgaaga tactagatga aggcgattaa aaaccgttta
    29461 aataagttga ataagttagc ggtggttgat gaagttgata ccgtgttttt gacgcagatt
    29521 gatactggac attttgaggt aagatatagg ataggtggaa aaaatcccaa agatattata
    29581 gaagtctatc cgacaaaaga agaagcagaa aaagctattt cagaaattaa aaatacaaaa
    29641 ggattgcgga tattctggga caatttggct ttattggagg ataattagag cttatcattt
    29701 atcaagcaga taagagaaat aataaccctc cttaactcct tgagaaacga caagcagata
    29761 agcgaagttt ttagccctct tatttcggtt ctactccttg cagaatgaca aggaaataag
    29821 agagataaaa acatccctta attgcttatc aaaccacaag gagatacggg ggctatctcc
    29881 ctccccacgc acttctatga gcttcacacc gtcattgtac attttttctc acggggaagc
    29941 atttgaagct agagcgaaca tagagaaaac gaaaaaagcc aagacactct ctgtctcagc
    30001 aataatctca ataatattat tatatcacaa aggaggccga gacatgacac cagagcaggt
    30061 aaaagaaaaa ctagagggcg ttaagtggat aaacaaattt aaaagtgtgt agattacaat
    30121 ctaacacaag aaaagaaaac gcccttttgg ttgttcttta attgcttcat caactatctg
    30181 agatgtttga taagaatatt tcattgaccg tctccaatta cacataggac acaatttatt
    30241 cttacaaaaa taggcttgat ataaacgcaa actcccatct tctcgacgaa taaatcgaag
    30301 agtatctcca caagtctcaa cgcgttctat caaggtttcc ctataaccta actctttaaa
    30361 aatatctgcc aatttcaaac ttaataattt tcgtcctcgc cagtttctat ccttgcctct
    30421 tttattttta tctaaaagaa ttttatcttg attttcccac ttctccatgt tataatttac
    30481 ctgtaattac aaagaaatac aaaaatacct ttgccttttt tctcttttta taattttttc
    30541 tgaacaacta aattataaaa gatccgttga aaaaagtcaa gggcattttt ttacctaaaa
    30601 agtcagtagt atcaagggtt tagaccctgt cgaaacccta aaaatagcat gagagttatt
    30661 tcctactagt atcaagataa gaaaaaacct actcttcgag taggtttttt acggagcttt
    30721 cagctccttt tatatatcta attaccgttt ttaccgtttt cctctatatg gaaatacaat
    30781 gaataaaaac aaaactaatg ctattgttaa cagtattttc atcggaatag ggagattatc
    30841 tagccatatt tgaatagaac caaaaagctt tatatctaaa actttatcaa aaaactttat
    30901 aaaaaaatat aagactatga cactccaaaa taaccaagct ttattcataa caaactcctt
    30961 gttatttaaa ggcagaatta aaatcgttta ctgatcaact tacactcctc tctaa
//
LOCUS       DAVLTM010000011        29168 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLTM010000011 DAVLTM010000000
VERSION     DAVLTM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.011"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..509
                     /locus_tag="VYN16_000436"
     CDS             <1..509
                     /locus_tag="VYN16_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403016.1"
                     /translation="HYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIYFIWLLIE
                     GLKYIRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLN
                     FLQNSDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEI
                     DKKLEISR"
     gene            565..1260
                     /locus_tag="VYN16_000437"
     CDS             565..1260
                     /locus_tag="VYN16_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403017.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1272..1688
                     /locus_tag="VYN16_000438"
     CDS             1272..1688
                     /locus_tag="VYN16_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403018.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
     gene            complement(2315..2407)
                     /locus_tag="VYN16_000439"
     CDS             complement(2315..2407)
                     /locus_tag="VYN16_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1403019.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            2568..3539
                     /gene="ruvB"
                     /locus_tag="VYN16_000440"
     CDS             2568..3539
                     /gene="ruvB"
                     /locus_tag="VYN16_000440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1403020.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            3628..4092
                     /locus_tag="VYN16_000441"
     CDS             3628..4092
                     /locus_tag="VYN16_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1403021.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            4323..5081
                     /locus_tag="VYN16_000442"
     CDS             4323..5081
                     /locus_tag="VYN16_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1403022.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            5090..5893
                     /locus_tag="VYN16_000443"
     CDS             5090..5893
                     /locus_tag="VYN16_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1403023.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLVMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            5915..7174
                     /gene="rseP"
                     /locus_tag="VYN16_000444"
     CDS             5915..7174
                     /gene="rseP"
                     /locus_tag="VYN16_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1403024.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            7187..9040
                     /locus_tag="VYN16_000445"
     CDS             7187..9040
                     /locus_tag="VYN16_000445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1403025.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            9140..10519
                     /gene="bglA"
                     /locus_tag="VYN16_000446"
     CDS             9140..10519
                     /gene="bglA"
                     /locus_tag="VYN16_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1403026.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGICLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            10711..12519
                     /gene="glmS"
                     /locus_tag="VYN16_000447"
     CDS             10711..12519
                     /gene="glmS"
                     /locus_tag="VYN16_000447"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1403027.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            12638..13687
                     /locus_tag="VYN16_000448"
     CDS             12638..13687
                     /locus_tag="VYN16_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1403028.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIDGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLDQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(13787..17584)
                     /gene="spuA"
                     /locus_tag="VYN16_000449"
     CDS             complement(13787..17584)
                     /gene="spuA"
                     /locus_tag="VYN16_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1403029.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVEIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSIPSK
                     PEHQNEASHPAYQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVQNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            18001..18414
                     /gene="rpsL"
                     /locus_tag="VYN16_000450"
     CDS             18001..18414
                     /gene="rpsL"
                     /locus_tag="VYN16_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1403030.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            18434..18904
                     /gene="rpsG"
                     /locus_tag="VYN16_000451"
     CDS             18434..18904
                     /gene="rpsG"
                     /locus_tag="VYN16_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1403031.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            19329..21410
                     /gene="fusA"
                     /locus_tag="VYN16_000452"
     CDS             19329..21410
                     /gene="fusA"
                     /locus_tag="VYN16_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1403032.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            21514..25905
                     /locus_tag="VYN16_000453"
     CDS             21514..25905
                     /locus_tag="VYN16_000453"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1403033.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKFLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEAKIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            26007..26270
                     /locus_tag="VYN16_000454"
     CDS             26007..26270
                     /locus_tag="VYN16_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1403034.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            26263..26541
                     /locus_tag="VYN16_000455"
     CDS             26263..26541
                     /locus_tag="VYN16_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1403035.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            26562..26714
                     /locus_tag="VYN16_000456"
     CDS             26562..26714
                     /locus_tag="VYN16_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403036.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            26736..27977
                     /locus_tag="VYN16_000457"
     CDS             26736..27977
                     /locus_tag="VYN16_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1403037.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     IFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            27987..28217
                     /locus_tag="VYN16_000458"
     CDS             27987..28217
                     /locus_tag="VYN16_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1403038.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            28440..29162
                     /locus_tag="VYN16_000459"
     CDS             28440..29162
                     /locus_tag="VYN16_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1403039.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         8267 a         5477 c         6886 g         8538 t
ORIGIN      
        1 ttcattattt tggagccaaa aatttaatag tttcttttaa tgaaacttat cctcgtattc
       61 aatccagtgt tggtatcatg tggctaccac tgtttggtat tctgataatc tattttattt
      121 ggctcttaat agagggatta aaatatatca gaaaaataaa ttatattatt caaaataggg
      181 aaataatcta tttttattcg caggtaaaag ttgtagataa acttactgat tctgaatcga
      241 aaaaatacgg tgaatatatt gatttgtacg aatcgtgtcg tcagaacaat ttaagtgttc
      301 tacttaactt tcttcaaaat tcggatgata aacgaggagt tattgctagt aactcagtga
      361 gaattatgtt tgaatctttg gataaagcag accaaattgc ggccctgaat atttgtagaa
      421 actatgtagc acgagaaaat gttttaaata aagaagatgc agaaaaattg ttaaatgaaa
      481 ttgataaaaa acttgaaatt agcagatagg acaattcaaa tagagttgag taaaaagttt
      541 gaaaagtgaa acaaggagaa agctatgcca gtaaatgaat atggtcagat gattggtgag
      601 tcaatggaag gttatacacc cggtgcactg ccttctattg atttcttaga agggcgttat
      661 gctaggatag aggctctctc ggtagaaaaa catgcggagg atttgctagc tgtttatggc
      721 ccggatactc ctcgggagat gtggacctat ctttttcagg agccagtagc agatatgggg
      781 gagctggtta gccttttaaa tcagatgttg gctcgtaagg accgttttta ctatgcaatc
      841 atagacaagg cgactggtaa ggctttggga actttttctc tcatgcgcat tgaccagaat
      901 aaccgagtaa tagaagtcgg agcagtcact ttttctccag agctaagggg tacacggata
      961 ggaactgagg ctcagtatct cctagctcgc tatatttttg aggagctaaa ctatcgtcgc
     1021 tacgagtgga aatgcgatgc cttaaatctg ccatccagac gagttgcgga gcgtttggga
     1081 tttgtctatg aaggaacctt ccgccaggcg gtggtttata aggggcgtac gagagatacg
     1141 gattggttgt ctatgattga tatggactgg cctcaagtca aagatcgttt ggaaacatgg
     1201 ctgcgtcctg aaaattttga taaaaatgga cagcagtaca agagcttgag agaactttaa
     1261 gaggtgttga gatgattact attaaaaagc aagaaattgt caagctagag gatgttttgc
     1321 atctctatca ggctgtcggt tggacaaatt atacccatca accagagatg ctggagcatg
     1381 ccttatcgca ttcattagta atttatctgg cacttgatgg tgatgctgtg gtgggcttga
     1441 ttcgtttggt tggagatgga ttctcatcag tttttgtaca ggatttgatt gttttaccaa
     1501 tctatcagcg tcaagggatt ggtagtgccc taatgaaaga ggccttaaaa gattacaaag
     1561 atgcttatca agtccagctg gtgacagaag agacagaaaa aaacgtggga ttttatcgtt
     1621 ctatgggctt tgaaatctta tccacctata attgtatagg gatgacttgg gcgaatcgag
     1681 aaaaataaca aaacttgttt tttcttaagc aaaatttaag gatggtctag tattatatag
     1741 tcattaaata aagacctcct aactttattt aataaaatcc taaacttttc tttttcataa
     1801 taatctccct taactccacc caatcaggtg gagtttttta gctctatttc aggcttttgg
     1861 ggactattct aaaaataatt tttcgatatt tttcggtatt tttcggattt tggtcgggga
     1921 attggcgggg acttttttag cgaatataac taagaaatag gtctgtggtc gcttcagcta
     1981 gttcaaactc aatttgattg taagtgactg ttaattgaag aactggaact actgtaataa
     2041 tgattttgct tgtctagtag tggcgtatta aatgagaagt gacatgtaag attgtctctt
     2101 cgtttacgaa atcaaaattt ctatgaaatt gatagaatcc ctaacgttct cttaatctta
     2161 accgtcttgt cgtacagcgg ttgaagaaaa tgtaaacctt tgacaaaatt cgtgaactat
     2221 gggataataa aaaggaattg agtactagtt ctatatcata ggctagaaaa gaccccaagc
     2281 tcacgaccaa ataagcttgg ggtctttttt gacactattt gtccttgttt agccatttat
     2341 cgactatacg aagaacgaca ccgaccacaa ttggtccgat gatagttttg agtactaatt
     2401 ccatcatggg ctatctcacc tcctttcgta ggcggtgtag cagtgtcgta gaatattata
     2461 ccacataagt gctaaactta ggagtcaccc aatcaggtgg cttttttgtt tgtggcttgg
     2521 ttttttgata taatagagcc atgagtagaa ttttagataa tgagataatg ggggatgagg
     2581 agttagtaga acgcacgctc cgtcctcagt atttacgtga atatattggg caggataagg
     2641 ttaaggacca gcttcaaatc tttatagaag ctgccaaaat gcgagatgaa gcgctggatc
     2701 atgtcctctt atttgggcct ccaggtttgg ggaaaacaac catggctttt gtcattgcca
     2761 atgaactggg agtcaatctc aagcaaacgt ctggtcctgt tatcgaaaaa gcgggtgatt
     2821 tggtagcgat tttgaatgag ttagagcctg gagatgtcct ctttattgac gagattcatc
     2881 gcttacccat gtcagtggaa gaagtgcttt atagtgccat ggaggacttc tacatcgata
     2941 ttatgattgg ggctggtgaa ggcagtcgca gtgttcattt ggagttgcct ccttttacct
     3001 tgattggtgc gacgactcgg gctggtatgc tctcaaatcc actacgggca cgttttggga
     3061 ttacaggtca tatggagtat tatgcccatg ctgacttgac agaaattgtc gagcggacgg
     3121 cagatatttt tgagatggaa atcactcatg aggcagcatc tgagttggcc ctacgtagtc
     3181 gtggaacccc tcgtattgcc aatcgtctcc tcaagcgcgt gcgcgatttt gcccagataa
     3241 tggggaatgg ggtaatcgat gatattatta ccgataaggc tttgactatg ctggatgttg
     3301 accatgaagg tttggactat gtggatcaaa aaatccttcg taccatgatt gagatgtaca
     3361 gtggaggacc tgttggtcta ggaactcttt ctgtcaatat cgccgaagag cgtgagacag
     3421 ttgaagacat gtatgagcct tacttgattc aaaaaggttt tatcatgcgg acacggtctg
     3481 gacgggtggc gactgctaag gcatatgagc acttaggtta tgaatacagt gaaaaataag
     3541 caagaaatct tagaggcttt tagagaaaat ccagatatga tggccattct gacgatcatc
     3601 cgagaccttg gtttgaaaga ctcgtagttg gcagcaggtt ctgtcagaaa tttcatctgg
     3661 aatcttttgt cagacaaatc cccttttgat catgaaacag atatagatgt gattttcttt
     3721 gatccagatt tttcttatga ggaaacctta ttactggaga aaaagctgag agaggatttt
     3781 cctcagtacc agtgggaatt gaaaaatcag gtctatatgc atcagcacag tcctcacact
     3841 gcttcctata ccagttctcg tgatgctatg agtaagtatc cagaacggtg tacggcagtt
     3901 ggactgcgct tgaatgaaga atcagatttt gaactctatg caccttatgg tttggaggat
     3961 attttgaatt ttcaagttcg tccaactcct catttcttag aaaatgaaga ccgaatggaa
     4021 ctctatcaaa cacgtttatc caagaaaaat tggcaggaga aatggaaaaa tttgattttt
     4081 aaaaatactt aaggaaactt taagctaggg agtgtacact aagttcataa gttaagaaga
     4141 ccttaactta aactcctaaa actttttcat aataatctcc ctataaaaat aaagtcgccc
     4201 aatcaggcgg cttatttttt tgaaaaatgg gcatggtgcc tgagaataaa tagcttagtg
     4261 atagaagaaa atggggaaat atggtataat gaaacgatag atttttgaat aggaataaga
     4321 tcatgtttgg attttttaag aaagataagg ctgtggaagt agaggttccg acacaggttc
     4381 ctgctcatat cggcatcatc atggatggca atggccgttg ggctaaaaaa cgtatgcaac
     4441 cgcgagtttt tggacataag gcgggcatgg aagcattgca aaccgtgacc aaggcagcca
     4501 acaaactggg cgtcaaggtt attacggtct atgctttttc tacggaaaac tggacccgtc
     4561 cagatcagga agtcaagttt atcatgaact tgccagtaga gttttatgat aattatgtcc
     4621 cggaactaca tgcgaataat gttaagattc aaatgattgg ggagacagac cgcctgccta
     4681 agcaaacctt tgaagcttta accaaggctg aggaattgac taagaacaac acaggattga
     4741 ttcttaattt tgctcttaac tatggtggac gtgctgagat tacacaggcg cttaagttga
     4801 tttcccagga tgttttagat gccaaaatca acccaggtga catcacagag gaattgattg
     4861 gtaactatct ctttactcag catttgccta aggacttacg agacccagac ttgattatcc
     4921 gtactagtgg agaattacgt ttgagcaatt tccttccatg gcagggagcc tatagtgagc
     4981 tttattttac ggacacctta tggcctgatt ttgacgaagc ggccttgcag gaagctattc
     5041 ttgcctataa tcgtcgtcat cgccgatttg gaggagttta ggaggaaata tgacccagga
     5101 tttacagaaa agaaccttgt ttgcagggat tgccctggct attttcctac caattttaat
     5161 gattgggggc ctcttgcttc agatagcaat cggaatcata gccatgctag tcatgcatga
     5221 acttttgaag atgagaggtc tagagaccat gacgatggag ggcctcttga ccctctttgc
     5281 aacctttgca ttgaccattc ccttggagaa ttacctgact tttttgccag ttgatgggaa
     5341 tgtggttgcc tatagtgttt tgatttcaat catgttagga acgaccgttt ttagcaagtc
     5401 ttatacgatt gaggatgcgg ttttccctct tgctatgagc ttctacgtgg gctttggatt
     5461 taatgcttta ctagatgctc gtgttgcagg tttggacaag gctctcttag ccttgtgtat
     5521 cgtctgggcg acagacagtg gtgcctatct tgttgggatg aactatggga aacgaaagtt
     5581 agcaccaagg gtatcgccta ataaaaccct tgagggtgcc ttgggtggta ttttaggagc
     5641 aattttagta accattatct ttatgatagt tgacagtaca gttgctcttc catatggaat
     5701 ttacaagatg tcagtctttg ctattttctt tagcattgct ggacaatttg gtgatttact
     5761 agaaagttcg atcaaacgtc attttggtgt taaggattct gggaaattta tccctggaca
     5821 tggtggtgtt ttggatcgtt tcgatagtat gttgcttgta tttccaatca tgcacttatt
     5881 tggactcttt taatcaaaag acggaggaaa cgctatgctc ggaattttaa cctttattct
     5941 ggttttcggg attattgtag tggtgcacga gttcgggcac ttctactttg ccaagaaatc
     6001 agggatttta gtacgtgaat ttgccatcgg tatgggacct aaaatttttg ctcacattgg
     6061 taaggatgga acggcctata ccattcgaat tttgcctctg ggtggctatg tccgcatggc
     6121 cggttggggt gatgatacaa ctgaaatcaa gacaggaacg cctgttagtt tgacacttgc
     6181 tgatgatggt aaggttaaac gcatcaatct ctcaggtaaa aaattggatc aaacagccct
     6241 ccctatgcag gtgacccagt ttgattttga agacaagctc tttatcaaag gattggttct
     6301 ggaagaagaa aaaacatttg cagtggatca cgatgcaacg gttgtggaag cagatggtac
     6361 tgaggttcgg attgcacctt tagatgttca atatcaaaat gcgactatct ggggcaaact
     6421 gattaccaat tttgcaggtc ctatgaacaa ttttatctta ggtgtcgttg ttttttgggt
     6481 tttaatcttt atgcagggtg gtgtcagaga tgttgatacc aatcagttcc atatcatgcc
     6541 ccaaggtgcc ttggccaagg taggagtacc agaaacggca caaattacca agattggctc
     6601 acatgaggtt agcaactggg aaagcttgat ccaagctgtg gaaacagaaa ccaaagataa
     6661 gacggcaccg actttggatg tgactatttc tgaaaagggg agtgacaaac aagtcactgt
     6721 tacacccgaa gatagtcaag gtcgttacct tctaggtgtt caaccggggg ttaagtcaga
     6781 ttttctatcc atgtttgtag gtggttttac aactgctgct gactcagctc tccgaattct
     6841 ctcagctctg aaaaatctga ttttccaacc ggatttgaac aagttgggtg gacctgttgc
     6901 tatctttaag gcaagtagtg atgctgctaa aaatggaatt gaaaatatct tgtacttctt
     6961 ggcaatgatt tccatcaata ttgggatttt taatcttatt ccgattccag ccttggatgg
     7021 tggtaagatt gtgctcaata tcctagaagc catccgccgc aaaccattga aacaagaaat
     7081 tgaaacctat gtcaccttgg ccggagtggt catcatggtt gtcttgatga ttgctgtgac
     7141 ttggaatgac attatgcgac tcttttttag ataatcgagg aatattatga aacaaagtaa
     7201 aatgcctatc ccaacgcttc gcgaaatgcc aagcgatgct caagttatca gccatgctct
     7261 tatgttgcgt gctggttatg ttcgccaagt ttcagcaggt gtttattctt atctaccact
     7321 tgccaaccgt gtgattgaaa aagctaaaaa catcatgcgc caagaattcg aaaagattgg
     7381 tgctgttgag atgttggctc cagcccttct tagtgcagaa ttgtggcgtg aatcaggtcg
     7441 ttacgaaacc tatggtgaag acctttacaa actgaaaaac cgtgaaaaat cagactttat
     7501 cttaggtcca actcacgaag aaacctttac agctattgtc cgtgattctg ttaaatctta
     7561 caagcaattg ccactcaacc tttatcaaat tcagcccaag tatcgtgatg aaaaacgccc
     7621 acgtaatgga cttcttcgta cacgtgagtt tatcatgaag gatgcttata gtttccacgc
     7681 taactatgat agtttggata gtgtttatga tgagtacaaa gcagcctatg agcgtatttt
     7741 cactcgtagt ggtttagact tcaaggctat tattggtgac ggtggagcca tgggtggtaa
     7801 ggatagccaa gaatttatgg ccattacatc tgctcgtaca gaccttgacc gctgggttgt
     7861 cttggacaag tcagttgcct catttgacga aattcctgca gaagtgcaag aagaaatcaa
     7921 ggcagaattg ctcaaatgga tagtctctgg tgaagatacc attgcttact caagtgagtc
     7981 tagctatgca gctaacttag aaatggcaac aaacgagtac aaaccaagca accgtgttgt
     8041 cgctgaagaa gaagttactc gtgttgcaac gccagatgtt aaatcaattg atgaagttgc
     8101 agctttcctc aatgttccag aagaacaaac gattaaaacc ctcttctaca ttgcagatgg
     8161 tgagcttgtt gcagcccttc tagttggaaa tgaccaactc aacgaagtca agttgaaaaa
     8221 ccacttggga gcagatttct ttgacgttgc tagcgaagaa gaagtggcga atgttgttca
     8281 agcaggattt ggttcacttg gaccagttgg tttgccagag aatattaaaa ttattgcaga
     8341 tcgtaaggtg caagatgttc gcaatgcagt tgtcggtgct aacgaagatg gctaccactt
     8401 gactggtgtg aacccaggcc gtgattttac tgcagaatat gtggatatcc gtgaagttcg
     8461 tgagggtgaa atttccccag atggacaagg tgtccttaac tttgcgcgtg gtattgagat
     8521 cggtcatatt ttcaaactcg gaactcgcta ctcagcaagc atgggagcag atgtcttgga
     8581 tgaaaatggt cgtgctgtgc caatcatcat gggatgttac ggtatcggtg tcagccgtct
     8641 tctttcagca gtgatggagc aacacgctcg cctctttgtt aacaaaacgc caaaaggtga
     8701 ataccgttac gcttggggaa tcaatttccc taaagaattg gcaccatttg atgtgcattt
     8761 gattactgtc aatgtcaagg atgaagaagc gcaagccttg acagaaaaac ttgaagcaag
     8821 cttgatggga gctggttatg aagtcttgac agatgaccgt aacgaacgtg tcggtgttaa
     8881 atttagcgat agtgacttga ttggtcttcc aatccgtatc actgttggta aaaaagcggc
     8941 cgatggcatt gtagaagtta agatcaaggc aactggtgac accattgagg ttcatgcaga
     9001 taacgtgctt gaaacgcttg aaatcctaag caagaaataa aaactataat cagaagaaaa
     9061 acaaggaaaa aatgtaacta gtttttacct tgttttttct gtataatggg agaaatgagt
     9121 agataaagag gtaaatgtta tgctaagatt tccaaaggat tttgtctggg gatcctctac
     9181 ttctggaccg cagacagaag gacgtgtagc tggtgacggt aagggagaca atctctggga
     9241 ttactggttc caagtggagc caaatcgtta ctataatggg attggttcag ataagacatc
     9301 gactttttat gaaaattggg agcgggatat tgagcttttg ttagagactg gtcacacagt
     9361 ctttcggact tctattcagt ggtcacggat ttttccacaa ggctgtggaa aagtcaaccc
     9421 tcaaggtgtg gatttttatc gtaaggtctt tgaggctatt aaggctaaag gaatttgtct
     9481 gttagtcaat ctctatcatt ttgatttacc ttttgccctt caagaggatg gtgatggttg
     9541 ggaaaataag gcgacagtct cagcctatga agactatgct cgtttttgtt ttgagactta
     9601 tggagattta gtggatcaat ggattacctt taacgagccc atcgttcctg tagaatttgg
     9661 ttatttttat gatgcccatt atccacataa ggtggatgca gaggcagctg ttaaggtagc
     9721 ctatcataca caattggcca gcagccgggc tgttaaggct tgccatgaac ttttgcctga
     9781 ttccaagatt gggattgtcc tcaacttgac accggcttat ccacgtagcc agcatcctgc
     9841 tgatgtcaag gcagctcgta ttgcggccct ttttcaggcc caatctttct tagatccatc
     9901 tgtcttgggg acttatccac aggagttggt agaaatcttg catgaacacg gtcttttacc
     9961 tgatgctaca gaggaagagt tggaactcat tcgtgataat acggtggact tccttggtgt
    10021 gaactactat caacctttgc gtgttatggc tcctcgattt gctaagcatc cagagagtcc
    10081 actcttacca gaacattttt acgagcctta tgtgatgcct ggacgtaaaa tcaattctca
    10141 tcgtggttgg gagatttatg agcaagggat ttatgacatc gcccaaaata tcaaggaaaa
    10201 ttatggcaat attgagtgga tgttgactga gaatggtatg ggtgttgaag gggaagaaaa
    10261 attccgtcaa gatggaatga ttcaagatga ttaccgtatt gactttgtaa aaggtcatct
    10321 tcgtgaactt caccgtgcca ttgaagatgg tgccaactgt aagggctact tgatttggac
    10381 ctttattgac tgctggtcat ggctcaacag ctataaaaat cgctatggtt tggtcgaatt
    10441 agacttggaa acgcaagaac gtcgtctgaa gaaatcaggc cactggttca agaaactaag
    10501 cgacaataat ggattttaaa aaggactctg actgattatc ccaatcggtc agagtttttt
    10561 gcttacagga gcttaatttg aactatacca atttttactc ttgacaagtg aaagaaacaa
    10621 gtatatactg tttttgctga ttctataaaa gatgaattag actataccaa ttttaaggag
    10681 aaagcacagc ttgcctgtgt cgtatatact atgtgtggaa ttgttggtgt tgttggaaac
    10741 acaaatgcaa ctgatatttt gattcaaggg cttgaaaagc ttgaataccg tggctatgat
    10801 tctgcgggaa tttttgtcct agatggtgct gataaccatt tggtgaaggc ggttggtcgt
    10861 attgcagaat tgtctgccaa gacagctggt gttgagggaa caactggtat cggacatact
    10921 cgttgggcta ctcacggaaa accaactgag gacaatgctc acccacaccg ctctgagaca
    10981 gaacgttttg tcttggtgca taatggggtg attgagaact atcttgaaat caaggaagaa
    11041 taccttgcag gtcaccactt caaggggcag acagatactg aaatagccgt tcatttgatt
    11101 ggaaaatttg cggaagaaga agggctctca gttcttgaag ccttcaaaaa agcccttcac
    11161 atcatccgtg gttcttatgc ctttgccttg gttgactcac aagatcctga agtcatctac
    11221 gtagctaaaa ataaatcacc acttttgatt ggtcttggag aaggctataa catggtctgc
    11281 tcagacgcca tggctatgat tcgtgaaact aaccaataca tggaaatcca tgaccaagag
    11341 ttggtaatcg tcaaggctga tagtgtggaa gttcaagact atgatggaaa tcgtcgtgag
    11401 cgtgctagct acactgctga acttgacttg tcagatatcg gtaagggaac ttacccttac
    11461 tacatgctca aggaaattga cgagcaacca actgttatgc gtaaactcat tcaagcctac
    11521 acggatgatg ctggtcaagt agtggttgat cctgctatca ttaaggctgt tcaagacgca
    11581 gaccgcatct acatccttgc agctggaaca tcttaccatg caggatttgc ttctaagaaa
    11641 atgttggaag aattgacaga tacaccagtt gaacttggca tctcatctga gtggggctac
    11701 ggtatgccac ttctcagcaa gaaaccactc ttcatcttta tcagccaatc tggtgaaaca
    11761 gcggatagtc gtcaagtttt ggtcaaggct aatgaaatgg gaattccaag cttaacagtg
    11821 acaaatgttc caggctcaac cctctcacgt gaagccaact ataccatgct ccttcacgca
    11881 ggtcctgaaa ttgccgtggc atcaactaaa gcctatacag cgcaaatcgc agcccttgcc
    11941 ttccttgcaa aagcagtcgg agaagcaaat ggtaatgcta aagcgcaagc ctttgacctg
    12001 attcatgaat tgtcaatcgt agctcagtct atcgaatcaa ctctttcaga gaaagaaacc
    12061 attgaagcca aggttcgtga acttcttgaa acaactcgta acgcctttta catcggacgt
    12121 ggtcaagatt actacgtagc catggaagca agtctcaaac tcaaagagat ttcttatatc
    12181 cagtgtgaag gctttgctgc aggagaactc aagcacggaa ccattgcctt gattgaagaa
    12241 ggaacgcctg ttttggctct cttgtcagat ccagttcttg ccaatcatac tcgtggaaat
    12301 atccaagagg tcgcagcccg tggtgccaaa gtcctcacta tcgcagaaga gaatgtagcc
    12361 aaagataccg acgatatcgt ccttacgacc gtacatccat acctctcacc aatttcaatg
    12421 gtcgtaccaa cgcaattagt cgcttacttt gcaaccctcc accgtggcct cgatgtggat
    12481 aaaccacgta accttgctaa atcagtaacg gtagaataag ctaaaaaagt ctagttatct
    12541 aggctttttc taatgagcaa attggttgct tgtacttaag attcgtgtta gaataatttt
    12601 tcaaattgaa ggacagagat agacaaggag aatcacaatg gtagaattgg gaatttcaac
    12661 atttggggaa ataacggagc ttgaagggac tggacaaact tacagtcatg ctgaacgcat
    12721 tcgccagttg gtggcagaga ttgagctggc tgacaaggtt ggtttggacg tatatgggat
    12781 tggcgagcac catcgggcgg attttgcagt atcagcccca gagattgtcc tggccgctgg
    12841 ggcagtcaat accaagaaaa tccgtttgac cagcgcagtc agcattctgt caagcatgga
    12901 cccgattcgt ttgttccaac agtatgccac tatcgatgct ttgtcaaatg gacgagcgga
    12961 gattatggct ggaaggggct ctttcacgga atctttccct ctgtttggtt atgacttgaa
    13021 agactacgac tctctttttg atgagaaatt agacttgctt cagttagtca atgaaaagac
    13081 caagttagac tggcaaggtc gattgactca aacgattgct ggtaaagaag tttatcctcg
    13141 tccagttcag gacaaattgc ccttgtggat agctacaggt ggtcatgtcg aatcaacagt
    13201 gaagattgct caggctggtc taccgattgt atatgctatt attgatggta atccacgtta
    13261 ttttaaaaag ttgattcagg cttatcgtga gattggaagc gaagcgggcc atgctgacaa
    13321 agacttgaaa gtgggagccc attcttgggg gtggattgcg gaagatggtg agcaggcggt
    13381 gaaagattat ttccatccga ctaagcaagt ggtggatgct atttccaaag accgtccgca
    13441 ctggcaggaa ttgcgttatg agcaatattt ggatcaggta gggtcaaatg gcgccatgtt
    13501 tgttggcaat ccagatcagg tggccgaaaa attgattcgc atgatagagg atttaggatt
    13561 ggaccgcttc atgcttcatc taccgcttgg ttctatgcct catgaccagg ttctgagagc
    13621 tattgaactc ttcggaacac aagttgcacc caaagtacga gcttattttg ctatgaaaga
    13681 ggcttaataa aaaatcctaa tcaagtttat taggacaaca aatattgtac gaaaattaat
    13741 cccccgagct ataatgcttg ggggattttt ctataggagg gctagtttag ttctcttttt
    13801 tattttttag taagaaaccg agaccaagga gcgcaaggag gctgattcct gcaaatagga
    13861 gtttgttttc gtttttgatt cctgtatttg gaagttcagc ttgtctatct ggggttgcag
    13921 gtatattttt cttgctagag ttttctaccg attcgttttg aaccgcttct tttacagatg
    13981 atgcttgtgc ttcttgtgct ggttgttcag cttgtgaatc agctgtagct tggctaggtt
    14041 tattttccgc atcagctact ttggcatctg gtttagtaga atcaggtcta gcttctggag
    14101 ctgggtcttg atatgcaggg tgagaagctt cattttgatg ttcaggcttg gaagggattg
    14161 agtctggttt ctcttctgca gttgactcat ggctagttcc attttgagag actcgaagaa
    14221 cagtagctgt aagggcattc aatttcaagc ctttttcagt ccattcaagt cctttcgggt
    14281 tggcaatttc gactgatcct gcttggtttt catctgccaa aacttccgca tttcttagat
    14341 gtgcaaaggc agttcccaaa ttaaattcgc gagctttttc atccgcattg acaaagactg
    14401 cgtagatatc gccgtttgga gcagtgattt ggtagccaat cactacatcc tctttttcca
    14461 caccattttg gcctgggaca gtgatgaggt ggacacggtc tttgatatct tgaagactct
    14521 taagtcggaa ggcatctgta gattgacgaa gggcaatcaa acctttcata tagtcacggc
    14581 tcttgacatt ttcaggataa gctttaccat ctgtagcctt agtccagtca aacttgttga
    14641 ctgcatcgct agaatcgtaa gaatcatgga tgaagtaagg atagtcaaat gggttgccgt
    14701 ccttatcacg caacaagtga gatttgtttg gaaccttatc ctctgctact ggagtcttgt
    14761 aggctgggtc acggaattgt ttagtacgtc catattcctg accggagtgg ataaatggag
    14821 ttccttgagc tgtcaagacc atgagatttc caagtcgtaa acgacggtgg atttcagcat
    14881 agttctcagc cttgcttggg tcttttttga tagactgggc aatgatgtca aagagggtca
    14941 agttatcatg ggctgcgatg tattggatga catctccagg gctgtcagct tcaaagttag
    15001 ttggttgagc aatgagattt ttaaagatgg tgttgacatc acgcttgcca cctgtgataa
    15061 aggcaggttg accttcgttt ggataaccag atttgaggtt gttacggatg tcatctgaaa
    15121 agacagcgac agtatcggta tgtttcatcc aatcttggtc agcagcttta gtaggcatgt
    15181 tttcatcacc ggcataggtt ctccaacctt caccaagcat gatgaggttt ggattgaggg
    15241 cgcgtgcagc cttgtaagct tcttcgatag aagcggcgtc atggtctccc atcatatcga
    15301 agcggaagcc atccactttg taggtatcaa ctaggtattt gatagagtca actaggagcc
    15361 gtttggtcat atggtgggtt gtccccaagc gtccaccacc aaagctagtt cgaggtgtgc
    15421 catctgcatc cataaagtgg tagtagtttg gctctaggtc ttcaaagata tcgactttgg
    15481 ctgtgtggtt ataaacgaca tctaggatag ctcccatacc acgtttgtgg atttcgttga
    15541 tgaggttttt aaattctgcg attcgttttt ctggattctt aggatcgctt gagtacatac
    15601 cagtcaagga gaagtagttt tgagggtcat atccccagtt gtagttgctg ttgcttgaag
    15661 cgtagtcaga caagcgttca tggtttttca attcattgac aaagtagtaa gacaagactg
    15721 gaaggagctg gatatgggtt acacccaagt ctttgagata gtctagtttt tcaatgaagg
    15781 cttcaaaagt cccaaatggt ttggtcaagt cttttgcaat ggcaggatct gaagtgaagt
    15841 cacgcacatg agcttcgtag ataacggcgt cttcacgagt cttgaaattg tgaatcttac
    15901 cataagtcaa gtcttgaggt ccgagtttag ctggatctac aaaggcggct ttagccactt
    15961 tatgggcatc gtcaatcttg gcatcgtcgc tattccaagc agcaagagat ttagcgtaag
    16021 gatcgagtgc aagaacagtt ttaccttgac gctcgatttg gtattgataa tagtatccag
    16081 tgaaatctgt gattccgagt ttgtttgtgc tgtctagagt ttgtttccaa gttcctcttt
    16141 cccctttttc aagagcgaca gttccaacta ctttgtcagg gtcattcttg tcgtagacaa
    16201 caacagaaac cttatcagca cttggtgacc aaagggtcaa atcaacttgt tttccttctt
    16261 cttttaggtc agctcccagt ttgccatcat agctgtatgt ctcatctttc aggcgccagc
    16321 ttgttttggt agtgaattgg tcggaattgt agctaacagt ataaggatgt tttgtgtcag
    16381 agaaatctcc gctgtaggtc actttcttac cagcttcatc gattgcaaca tcggtaatag
    16441 ttaccttgtt tcctaggtga ttagtgatgt tggagtgttt gaggatatct tcttttttag
    16501 caccgacaag tgttgaaaag ctactttcaa tgctagaagt gcctacgtgt tgggctcctg
    16561 tcatacggat atcatggaca tagtatggat ttgtgtaaat cgattcatca tcgtctttta
    16621 ggaaaatttg gctatgattt ttcaaatctg tgaacttata attttcttta cggattttca
    16681 cgtcgtctcc ttgtttgctc tcatctagta ataaaaatcc aaattctctt gcggcttcat
    16741 taagaggaat gtcgatatag cggccatatt tgcctgtagc cgtaaagtct gttccgtcag
    16801 gccattgagc gctacttgga ttttttacat ctccccagta ccagagagat ttcttgtcat
    16861 agttgccatc tgtgcggtag tagttgacgc gaacagttcc tgcaggctgt ggctcgtaag
    16921 agaaaacctt gtaatcttgg tctaaccaag cctcgttcat ttttggaact agtttttcta
    16981 cagatttatc gccggttaga ttttttccag ctgtattgtt gatgaggaag ctaattttct
    17041 tggcttgttc tccctttaat ttgacatcta ggtaatagcc gtagtcatct ttcttggcat
    17101 ccttgaagga caaagctccg tttggccagt tttcagatgg tttttcaaca tcgtcccaag
    17161 tccatagtcc ttgagcatcc ttgttttctt caggaagttt tttgacatgg atacggaagt
    17221 agttatcttc gatgggctct tctgccttag tttcagttgt tactggacta gtagaagtag
    17281 ttggctctgt tgcaggtttt tcttctgttt tctctaggga agcgtttgca tttctaggtg
    17341 aagaaatatc actttcgttc ttaccagagc cagctgctgt atcagtaaga ggttgggcaa
    17401 gggtggttgt atttctgttg ttgggttcgt tagtggttgg tgtactttcg tcggatgaga
    17461 tagttggtgt agccatggca agcaagacaa ggcttgcgcc gataaggaca gaaccagttc
    17521 catttttgag ggaacggatg ctgtagacca tttttttctc agtgtgagat ggtgtttttc
    17581 tcataatgat tctccaatca ataaatttct atatcagtat agcatgtagt aaaataatat
    17641 gcaagcgttt tctttgattt gtgaaaaaaa ttcttacttt tgagaagttt tctgtactat
    17701 tttatatatc agtataaatg acggagataa aaatgtcaca taagccaagc cgtattatta
    17761 ggaagatgat gtgttcccag ctatttttat tcagaaattg atctctaaca ttaccaataa
    17821 aaatgaaaaa tatcttgaca agcaagggaa aattttgtta caatgataga cggtactttt
    17881 tacttttggt ctctcaagag tgtacaggga cgtgctgaca aatgttgcaa aagtacacac
    17941 agatgatatc tgtcaccaag tgtatcatca ccaaaaataa aaaaacacag gagaatgtag
    18001 atgcctacaa ttaaccaatt ggttcgcaaa ccgcgtaaat caaaagtaga aaaatctaaa
    18061 tcaccagctt tgaacgttgg ttacaatagt cataaaaaag ttcaaacaaa cgtttcttca
    18121 ccacaaaaac gtggtgttgc aactcgtgtt ggaacaatga cacctaaaaa acctaactca
    18181 gcccttcgta aattcgctcg tgtacgtttg agcaacctta tcgaagttac tgcctacatc
    18241 ccaggtatcg gacacaactt gcaagagcac agcgtggtgc ttcttcgcgg tggacgtgta
    18301 aaagaccttc caggggtacg ttaccatatc gtccgtggtg cacttgatac tgcaggtgtt
    18361 aacgatcgta aacaaggccg ttctaaatac ggtactaaac gtccaaaagc ataaggaaag
    18421 gggataaaga gaaatgagtc gtaaaaatag agctccaaaa cgtgacgtat tgccagatcc
    18481 gctatacaat tcacaactag ttactcgtct tatcaaccgc gttatgcttg atggtaaacg
    18541 tggtactgct gcttcaatcg tttacggtgc ttttgagcaa atcaaagaag ctactggcaa
    18601 cgatgcactt gaagtatttg aaacagctat ggaaaacatc atgcctgtac ttgaagtacg
    18661 tgcacgtcgt gttggtggtt ctaactacca agtcccagtt gaagttcgtc cagaacgtcg
    18721 tacaacactt ggacttcgtt ggttggtaac aatcgctcgt cttcgtggtg aacacacaat
    18781 gcaagaccgt cttgcaaaag aaatcttgga tgctgctaac aacactggtg cagctgttaa
    18841 gaaacgtgaa gatactcacc gtatggctga agctaaccgt gcattcgcac acttccgttg
    18901 gtaagatagg atgcgaaagc gttaagaaag tcccagagaa aatagggaat cgaagcaggt
    18961 tgcggttgca accaatgaga ttcatctttt tctccagact tttagcttga gctcaactaa
    19021 atcatgatgc taggcacggt aaggatgcaa ggtgaaaata ggaaactgac gcagtattca
    19081 acgaatacaa ggagttttat ctttttcacg cagcatcccg ttccagctca catcggctaa
    19141 ctaactttag cccgggttca aattagctaa atcgattagt attagctata actcagctta
    19201 ccatctagta agttgaaacc aacaatagca tgaaaacatt gagaacgggt aggtcctgcc
    19261 tatccgtttt tattaaaatc gtgttataat agaatagaaa tcaaaaataa ataggagaaa
    19321 caaacctcat ggcacgcgaa ttttcacttg aaaaaactcg taatatcggt atcatggctc
    19381 acgtcgatgc cggtaaaaca acaactactg agcgtattct ttactacact ggtaaaatcc
    19441 acaaaatcgg tgaaactcac gaaggtgcgt cacaaatgga ctggatggag caagagcaag
    19501 aacgtggtat cacgatcaca tctgctgcga caacagctca atggaacaac caccgcgtaa
    19561 acatcatcga cacaccagga cacgtggact tcacaatcga agtacaacgt tctcttcgtg
    19621 tattggatgg tgcggttacc gttcttgact cacaatcagg tgttgaacct caaactgaaa
    19681 cagtttggcg tcaagcaact gagtacggag ttccacgtat cgtatttgcc aacaaaatgg
    19741 acaaaatcgg tgctgacttc ctttactctg taagcacact tcacgatcgt cttcaagcaa
    19801 atgcacaccc aatccaattg ccaatcggtt ctgaagatga cttccgtggt atcattgact
    19861 tgatcaagat gaaagctgaa atctatacta acgaccttgg tacggatatc cttgaagaag
    19921 acatcccagc tgaatacctt gaccaagctc aagaataccg tgaaaaattg attgaagcag
    19981 ttgctgaaac tgacgaagaa ttgatgatga aatacctcga aggtgaagaa atcactaacg
    20041 aagaattgaa agctggtatc cgtaaagcga ctatcaacgt tgaattcttt ccagtattgt
    20101 gtggttcagc cttcaaaaac aaaggtgttc aattgatgct tgatgcggtt atcgactacc
    20161 ttccaagccc acttgacatc ccagcaatca aaggtattaa cccagataca gacgctgaag
    20221 aaactcgtcc agcatctgac gaagagccat ttgcagctct tgccttcaag atcatgactg
    20281 acccattcgt aggtcgtttg acattcttcc gtgtttactc aggtgttctt caatcaggtt
    20341 catacgtatt gaatacttct aaaggtaaac gtgaacgtat cggacgtatc cttcaaatgc
    20401 acgctaacag ccgtcaagaa atcgacactg tttactcagg tgatatcgct gctgccgttg
    20461 gtttgaaaga tactacaact ggtgactcat tgacagatga aaaatctaaa atcatccttg
    20521 agtcaatcaa cgttccagaa ccagttatcc aattgatggt tgagccaaaa tctaaagctg
    20581 accaagacaa gatgggtatc gcccttcaaa aattggctga agaagatcca acattccgcg
    20641 ttgaaacaaa cgttgaaact ggtgaaacag ttatctcagg tatgggtgaa cttcaccttg
    20701 acgtccttgt tgatcgtatg cgtcgtgagt tcaaagttga agcgaacgta ggtgctcctc
    20761 aagtatctta ccgtgaaaca ttccgcgctt ctacccaagc acgtggattc ttcaaacgtc
    20821 agtctggtgg taaaggtcaa ttcggtgatg tgtggattga atttactcca aacgaagaag
    20881 gtaaaggatt cgaattcgaa aacgcaatcg tcggtggtgt ggttcctcgt gaatttatcc
    20941 cagcggttga aaaaggtttg gtagaatcta tggctaacgg tgttcttgca ggttacccaa
    21001 tggttgacgt taaagctaag ctttatgatg gttcatatca cgatgtcgac tcatctgaaa
    21061 ctgccttcaa gattgcggct tcactttccc ttaaagaagc tgctaaatca gcacaaccag
    21121 ctatccttga accaatgatg cttgtaacaa tcactgttcc agaagaaaac cttggtgatg
    21181 ttatgggtca cgtaactgct cgtcgtggac gtgtagatgg tatggaagca cacggtaaca
    21241 gccaaatcgt tcgtgcttac gttccacttg ctgaaatgtt cggttacgca acagttcttc
    21301 gttctgcatc tcaaggacgt ggtacattca tgatggtatt tgaccactac gaagatgtac
    21361 ctaagtcagt acaagaagaa attattaaga aaaataaagg tgaagactaa tccgtcctca
    21421 ctctagaagg aagtcactta gtggcttcct tttgtcttta gaaaatacct ctaaatatgg
    21481 taaaatagta gaagaataat gtgaggaaaa tgaatgtcaa atagttttga aattttgatg
    21541 aatcaattgg ggatgcctgc tgaaatgaga caggctcctg ctttagcaca ggccaatatt
    21601 gagcgagttg tggttcataa aattagtaag gtatgggagt ttcattttgt attttctaat
    21661 attttaccga ttgaaatctt tttagaatta aagaaaggtt tgagcgaaga attttctaag
    21721 acaggcaata aagctgtttt tgaaattaag gctcggtctc aagaattttc aaatcagctc
    21781 ttgcagtcct actataggga ggctttctct gaaggtccat gtgctagtca aggttttaag
    21841 ttcctttatc aaaatttgca agttcgtgct gagggtaatc agctatttat tgaaggatct
    21901 gaagcgattg ataaggaaca ttttaagaag aatcatcttc ctaatttagc taaacaactt
    21961 gaaaagtttg gttttccaac ttttaactgt caagtcgaga agaatgatgt cctgacccaa
    22021 gagcaggaag aggcctttca tgctgaaaat gagcagattg ttcaagctgc caatgaggaa
    22081 gcgctccgtg ctatggaaca actggagcag atggcacctc ctccagcgga agagaaacca
    22141 gtctttgatt ttcaagcgaa aaaagctgca gctaaaccca agctggataa ggcggagatt
    22201 actcctatga tcgaagtgac gacagaggaa aatcgtctgg tatttgaagg ggttgttttt
    22261 gatgtggagc aaaaagtgac tagaacaggt cgtgttttaa tcaactttaa aatgacggac
    22321 tatacttcaa gtttttctat gcaaaagtgg gttaaaaacg aggaagaggc ccagaagttt
    22381 gacctcatca agaagaattc ttggctccga gttcgaggga atgtggagat gaataacttc
    22441 acacgcgatt tgactatgaa cgtacaggat ctgcaggaag ttgttcacta tgagcggaag
    22501 gatttgatgc cagaaggtga gcgtcgggtt gagtttcatg ctcatactaa catgtcgact
    22561 atggatgctt tgccagaggt cgaagagatt gttgcaacag ctgctaagtg gggacacaag
    22621 gcggttgcta tcacagacca tgggaatgtc cagtcctttc cacatggcta taaggcggct
    22681 aagaaagcgg gaatccagct gatctatggg atggaagcca agatcgtgga ggaccgtgtc
    22741 cctatcgtct ataacgaagt ggagatggac ttatcagaag caacctacgt ggtctttgac
    22801 gtggaaacga cgggactttc agctatctat aatgacttga ttcaggttgc ggcctctaag
    22861 atgtacaagg ggaatgttat tgctgaattt gatgaattta tcaatcctgg gcatcccttg
    22921 tcagctttta ctacagagtt aactggaatt acagatgatc atgtcaaaaa tgccaaacca
    22981 ctagaacaag ttttgcaaga attccaagaa ttttgcaagg atacggtcct agttgcccac
    23041 aatgctacct ttgacgttgg ctttatgaat gctaattatg agcgtcatga tcttccaaag
    23101 attagtcagc cagttattga tacgctggag tttgctagaa acctctatcc tgagtataaa
    23161 cgccatggtt tggggccttt gaccaagcgt tttggtgtgg ccttggaaca tcaccacatg
    23221 gccaactacg atgcggaagc gactggtcgt ctgcttttca tctttatcaa agaggtagca
    23281 gaaaaacatg gtgtgaccga tttagctaga ctcaacattg atctaatcag tccagattct
    23341 tacaaaaaag ctcggatcaa gcatgcgacc atctatgtca agaatcaggt aggtctaaaa
    23401 aatatcttta agctggtttc cttgtctaat accaagtatt ttgaaggagt gccacggatt
    23461 ccgagaacgg ttctagatgc ccatcgagag ggcttgattt taggttcagc ctgttcagag
    23521 ggtgaagttt ttgacgtggt cgtttctcaa ggtgtggatg cggcggttga ggtggccaag
    23581 tattatgact ttatcgaggt catgccaccg gctatctatg cgcccttgat tgccaaagag
    23641 caggtcaagg atatggagga actccagacc attatcaaga gtttgataga ggttggagat
    23701 cgccttggca agcctgttct ggctacggga aatgttcact atatcgaacc ggaagaagag
    23761 atttatcgtg aaattatcgt ccgtagtttg ggacagggtg cgatgattaa ccgaactatc
    23821 ggtcatggtg aacatgccca accagcgcca cttccaaagg ctcattttcg aacgactaat
    23881 gagatgttgg atgaatttgc ctttttggga gaggaactgg ctcgtaaact ggttattgaa
    23941 aacaccaatg ccttggcaga aatatttgaa cccgttgaag tcgttaaggg tgacttgtat
    24001 acgcctttca tcgacaaggc tgaagaaaca gttgctgagt tgacctataa gaaagctttt
    24061 gagatttatg gaaatccgct gccagatatt gttgatttgc ggattgaaaa agaattaaca
    24121 tccatactgg ggaatggatt tgctgtgatt tatctggcat cgcagatgct ggtgcaacgt
    24181 tctaatgaac ggggttattt ggttggttct cgtgggtctg tcggatctag tttcgttgcg
    24241 accatgattg ggattacgga ggtcaatcct ctctctcctc actatgtctg tggtcagtgt
    24301 cagtacagtg agtttatcac agatggttcg tacggttcag gatttgatat gccccataag
    24361 gactgtccaa actgtggtca caaactcagt aaaaacggac aggatattcc gtttgagacc
    24421 ttccttggtt ttgatgggga taaggttcct gatattgact tgaacttctc gggagaagat
    24481 cagcctagcg cccacttgga tgtgcgtgat atctttggtg aagaatatgc cttccgtgcg
    24541 ggaacggttg gtacggtagc tgccaagact gcctatggat ttgtcaaggg ttacgagcga
    24601 gattatggca agttttatcg tgatgcagaa gtagaacgcc tcgctcaagg agcggcgggt
    24661 gtcaagcgga caacaggcca acacccgggg ggaatcgttg ttattccgaa ctacatggat
    24721 gtctacgatt ttacgcctgt ccagtatcca gcagatgatg tcacggctga atggcagacc
    24781 actcacttta acttccacga tatcgatgag aacgtcctca aactcgatgt actgggacat
    24841 gatgatccga ccatgattcg aaaacttcag gatttgtctg gtattgaccc taataaaatt
    24901 cctatggatg acgaaggcgt gatggcactc ttttctggga ctgatgtact aggggtaaca
    24961 cctgaacaaa ttggaacgcc tacgggtatg ttggggattc cagagtttgg aacaaatttc
    25021 gtacgtggaa tggtagacga aacccatccg acaacctttg cggaattgct tcagctgtct
    25081 ggtctgtccc acggtactga cgtttggttg gggaatgctc aggatctgat taagcaagga
    25141 atagcagacc tatcgactgt tatcggttgt cgggacgaca tcatggttta cctcatgcat
    25201 gcgggtctgg aacctaagat ggcttttacc attatggaac gggtacgtaa gggtttgtgg
    25261 ctaaagattt cagaagagga gagaaatggc tatatcgaag ccatgaaggc taataaggtg
    25321 ccagagtggt atatcgaatc ctgtgggaaa attaagtaca tgttccctaa ggcccatgcg
    25381 gcagcctacg ttatgatggc cttgcgtgta gcttacttca aggttcacca tcctatttat
    25441 tactactgtg cttacttctc cattcgtgct aaggcttttg atatcaagac catgggtgcg
    25501 ggcttggagg tcatcaagcg cagaatggaa gaaatctctg aaaaacggaa gaacaatgaa
    25561 gcctctaatg tggaaatcga tctctataca actcttgaga ttgtcaatga gatgtgggaa
    25621 cgaggtttca agtttggtaa attagatctc taccgtagtc aggcgacaga gttcctcatc
    25681 gacggggata cccttatccc accatttgta gcaatggatg gtctgggaga gaacgttgcc
    25741 aagcaactgg tgcgggcgcg tgaagaggga gaattcctct ctaaaacaga actacgcaag
    25801 cgtggtggac tctcatcaac cttggttgaa aagatggatg agatgggtat tcttggaaat
    25861 atgccagagg ataaccagtt gagtttgttt gatgagttgt tttaaaaaat tgcttaataa
    25921 tctattaaaa gaggctaacg tatatccaat agatttacat tagctttctt ttttgttaaa
    25981 atagtctatg gaaagagggt gagagtatgt caaagatgag tataagcatc cgtctggata
    26041 gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct gggaatggat atgacaacag
    26101 ctattaatat tttccttcgt caggcaattc aatatcaggg attacctttt gatgttagac
    26161 tagacgaaaa tcggaagttg ctccaagcgt taacggattt agaccaaaat cgtaatatga
    26221 gccagtcttt tgaatcagtc tcagatttga tggaggactt acgtgcttaa gattcgttat
    26281 cataaacagt ttaaaaaaga ttttaagttg gctatgaagc gtggtttgaa ggcagaatta
    26341 ttagaagaag ttttgaattt tctggttcaa gaaaaagaac atcctgccag atatcgtgat
    26401 cattcattga cggcatccaa gcattttcaa ggagttcgtg aatgccatac ccagccagat
    26461 tggcttttgg tttataaagt agacaagtcg gaattgattt taaatttgct gaggacaggc
    26521 agtcacagtg atttatttta atctatttta agggggttct catgaaacta agaatatttg
    26581 cggaagataa gccggctaag aaggtatttg aatatcaatt agaacttgct gatcgtacaa
    26641 ttcttctatc gacagcactc ttgtcaggtg ctattgcttt agcaggaatc ttttctgctt
    26701 tgaaagaaaa ataaaaatag aaaagagaaa acagaatggt tttaccaaat tttaaagaaa
    26761 atctagaaaa atatgcgaaa ttgttggttg cgaacggaat taacgtgcaa cctggtcaca
    26821 ctttggctct ctctattgat gtggagcaac gtgaattggc acatctaatc gtgaaagaag
    26881 cttatgcctt gggtgcgcat gaggtcatcg ttcagtggac agatgatgtg attaaccgtg
    26941 agaaattcct ccatgccccg atggagcgtt tggacaatgt gccagaatac aagattgctg
    27001 agatgaacta tcttttggag aacaaggcta gccgtcttgg ggttcgttca tctgatccag
    27061 gtgccttgaa cggagtggac gctgacaagc tttcagcttc tgctaaagct atgggacttg
    27121 ccatgaagcc aatgcgaatc gcaactcaat ctaacaaggt tagctggact gtagcagccg
    27181 ctgctggact tgagtgggct aagaaagtct tcccaaatgc tgcgagcgac gaagaagcag
    27241 ttgatttcct ttgggatcaa atcttcaaaa cttgtcgtgt ctacgaagca gatcctgtta
    27301 aggcttggga ggaacatgca gccattctca agagcaaggc cgatatgctt aataaggagc
    27361 aattttcagc ccttcactac acagcgccag gaacagattt aacacttggt ttgccaaaga
    27421 accacgtttg ggaatcagct ggtgctgtca atgcacaggg cgaagaattc ttgccaaata
    27481 tgccgacaga agaggtcttc acagcgcctg acttccgtcg tgcagatggt tatgtcactt
    27541 ctacaaaacc gcttagctac aacggaaata tcattgaagg cattaaggtg acctttaagg
    27601 atggacaaat cgtagatatc actgctgaga agggtgatca ggttatgaaa gaccttgtct
    27661 ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt ggtaccagat ccaagtccaa
    27721 tttctcagtc aggcattatc ttctttaaca cccttttcga tgaaaatgcg tcaaaccact
    27781 tggctatcgg tgcagcctat gcgactagcg ttgttgatgg agcggagatg agcgaagagg
    27841 agcttgaagc tgcagggctt aaccgttcag atgttcacgt agactttatg attggttcta
    27901 accaaatgga tatcgatggt attcgtgagg atggaacgcg ggtacctctt ttccgtaatg
    27961 ggaattgggc aaattaagga gataatatgt taggaagtat gttcgttggt ctcctagtgg
    28021 gatttttagc aggtgctatg accaatcgtg gagagcgaat gggatgtttt ggaaaaatgt
    28081 ttctcggttg gatcggagcc tttctaggtc acttgctctt tggaacttgg gggccagttt
    28141 tatcaggaac agctattatc ccagcgattt taggagccat gattgtttta gctatttttt
    28201 ggagacgagg aagttaattt cctaaatctg atactcaatg aaaatcaaag agcaaactag
    28261 gaaactagcc gcaggttgct caaagcactg ctttgaggtt gcagatagaa ctgacgaagt
    28321 cagtaacata tatacggcaa ggcgacgttg acgcggtttg aagagatttt tgaagagtat
    28381 gaaacgaaaa ggaggttggt cattgtacca gcctcctttt gatatgatat aatagttcta
    28441 tgagattaga taaattttta gttgcctgtg cggtgggaag tcggactgag gtcaaaaact
    28501 tgctcaaggc tgggcgcgtg actgtaaatg gtaaaaaaga aaaatcagct aaattgcaga
    28561 ttgatgaaaa aatagatgag attcgctttg atgggcaagt gttggagtat gaagagtttg
    28621 tctactacat gatgaacaag cccaaaggag ttatctcagc gactgaggat cccaagcaca
    28681 gaaccgttct ggacttgttg gatgacttgg cgcggagtaa ggaagttttc ccagtaggac
    28741 gcttggatat tgacacgcat ggtcttttgc tcttgaccaa tgatggtcag ctggctcatg
    28801 ttcttctttc gcccaagcgt catgtggata agacttatct ggcacaagtc aagggaatca
    28861 tgacccaaga agatgtggag acatttgctg agggtattcc tctcaaagac tttacctgtc
    28921 aacccgctat actggagctt gtatccatag atacagaaaa gaatcagagc caaatccgtg
    28981 tgaccattgc agaagggaag tttcatcaga tcaagcgtat ggtgggctac tgtggcaagg
    29041 aagtggtgga cttgcaacga ttgactatgg gaactttagt attagatgag aacctggaac
    29101 gaggggaatg gcgtcgcttg accaaggaag aattagaaat tctccgcgct aatattattt
    29161 aatttagt
//
LOCUS       DAVLTM010000012        29046 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLTM010000012 DAVLTM010000000
VERSION     DAVLTM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.012"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            89..523
                     /locus_tag="VYN16_000460"
     CDS             89..523
                     /locus_tag="VYN16_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403040.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVVAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            535..1725
                     /locus_tag="VYN16_000461"
     CDS             535..1725
                     /locus_tag="VYN16_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1403041.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1736..2227
                     /locus_tag="VYN16_000462"
     CDS             1736..2227
                     /locus_tag="VYN16_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1403042.1"
                     /translation="MTMPNIVMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2242..3021
                     /locus_tag="VYN16_000463"
     CDS             2242..3021
                     /locus_tag="VYN16_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1403043.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFVFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3008..3826
                     /locus_tag="VYN16_000464"
     CDS             3008..3826
                     /locus_tag="VYN16_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1403044.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3826..4119
                     /gene="yajC"
                     /locus_tag="VYN16_000465"
     CDS             3826..4119
                     /gene="yajC"
                     /locus_tag="VYN16_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1403045.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            4141..6042
                     /locus_tag="VYN16_000466"
     CDS             4141..6042
                     /locus_tag="VYN16_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1403046.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6102..7103
                     /locus_tag="VYN16_000467"
     CDS             6102..7103
                     /locus_tag="VYN16_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1403047.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7213..7548)
                     /locus_tag="VYN16_000468"
     CDS             complement(7213..7548)
                     /locus_tag="VYN16_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403048.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7559..7801)
                     /locus_tag="VYN16_000469"
     CDS             complement(7559..7801)
                     /locus_tag="VYN16_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403049.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTIIFFIIPPIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7811..8353)
                     /locus_tag="VYN16_000470"
     CDS             complement(7811..8353)
                     /locus_tag="VYN16_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1403050.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKAGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLGILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKDPDED"
     gene            complement(8369..8563)
                     /locus_tag="VYN16_000471"
     CDS             complement(8369..8563)
                     /locus_tag="VYN16_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403051.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8729..9679
                     /gene="rsmH"
                     /locus_tag="VYN16_000472"
     CDS             8729..9679
                     /gene="rsmH"
                     /locus_tag="VYN16_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1403052.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQQRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQEASLTAYEVVNHYDYHDLIRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMEMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9691..10008
                     /gene="ftsL"
                     /locus_tag="VYN16_000473"
     CDS             9691..10008
                     /gene="ftsL"
                     /locus_tag="VYN16_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1403053.1"
                     /translation="MAERMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            10012..12264
                     /locus_tag="VYN16_000474"
     CDS             10012..12264
                     /locus_tag="VYN16_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV1403054.1"
                     /translation="MKWTKKVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGSKGNGITY
                     ANMMAIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGKDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSTLQSFMETQ
                     MNAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGLTKDFVWRDILYQSNYE
                     PGSTMKVMTLAAAIDNNTFPGGEVFNSSELKVADATIRDWDVNEGLTGGGMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGIPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFITALYDPNNQSVRKSQKEIVGNPVSKDAA
                     SQTRTHMVLVGTDPRYGTMYNHSTGKATVNVPGQNVALKSGTAEIADEKNGGYLVGST
                     NNIFSVVAMNPAENPDFILYVTVQQPEHYSGIQLGEFANPILERAVAMKDSLNLQSTA
                     KTLDQVTNQSAYAMPSIKDISPGDLAEALRRNIVQPIVVGTGTKIKESSVEEGSNLAP
                     NQQVLLLSDKAEEVPDMYGWTKATAEAFSKWLNIELVFEGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12266..13246
                     /locus_tag="VYN16_000475"
     CDS             12266..13246
                     /locus_tag="VYN16_000475"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1403055.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIKFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIASVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVVFYLFYERGGDMLSVFGYQVHLGIFYILFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13855..15960
                     /locus_tag="VYN16_000476"
     CDS             13855..15960
                     /locus_tag="VYN16_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1403056.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(16256..16738)
                     /locus_tag="VYN16_000477"
     CDS             complement(16256..16738)
                     /locus_tag="VYN16_000477"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1403057.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16833..18317)
                     /locus_tag="VYN16_000478"
     CDS             complement(16833..18317)
                     /locus_tag="VYN16_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1403058.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            18470..20080
                     /locus_tag="VYN16_000479"
     CDS             18470..20080
                     /locus_tag="VYN16_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1403059.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDFLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLAQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEEINVQEALANSDSIFYTYQKL
                     IQIRKENSWLIRADFKLLDTADKVFAYIRKDGDRRFLVVANLSNDKQNFSVDGKVRSV
                     LIENTAAKEVLEKQVLTPWDAFCVEMTD"
     gene            complement(20158..20992)
                     /locus_tag="VYN16_000480"
                     /pseudo
     CDS             complement(20158..20992)
                     /locus_tag="VYN16_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20940..22325)
                     /locus_tag="VYN16_000481"
                     /pseudo
     CDS             complement(20940..22325)
                     /locus_tag="VYN16_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            22502..23947
                     /locus_tag="VYN16_000482"
     CDS             22502..23947
                     /locus_tag="VYN16_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1403060.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYFNLVVTALVLLVALVGLLLIIHKKAAKFTIFLLVLSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNIDLT
                     VNQSSSYLATYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDIHYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSMIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGRGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            23949..24680
                     /locus_tag="VYN16_000483"
     CDS             23949..24680
                     /locus_tag="VYN16_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1403061.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLGKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALIKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGEPYKFMKKRAQYFLERDLVHVIASDMHNVDSRPPHMAEAYDLVS
                     QKYGETKAQDLFIDNPRKIVMDQLI"
     gene            24689..25381
                     /locus_tag="VYN16_000484"
     CDS             24689..25381
                     /locus_tag="VYN16_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEV1403062.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKNKLIILLVALVTGAGAFAYSIFIV
                     KPEYTSTTRIYVVNRNQENKPGLTNQDLQAGTYLVKDYHEIILSQDVLEKVATNLKLD
                     IPVKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSIREVAAEKIIAVTRVSDVT
                     TLEEARPATTPSSPNVRRNTLVGFLGAAAVTVITVLLIELFDTRVKRPEEVEDVLQMP
                     LLGVVPDFNKMK"
     gene            25391..26080
                     /locus_tag="VYN16_000485"
     CDS             25391..26080
                     /locus_tag="VYN16_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1403063.1"
                     /translation="MPTLEISQAKLELAKKTEEYYNALCTNLQLSGDDLKVFSISSVK
                     AGEGKTTTSTNIAWAFAHAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNVENLFVIQAGSVSPNPIALLQSKNFSTMLGTLRKYFDYIVVDTAPIGIVI
                     DAAIIMQKCDASILVTKAGETKRRELQKAKEQLEQTGKSCLGVVLNKFDTSVDKYGFY
                     GSYGSYRKQKK"
     gene            26095..27456
                     /locus_tag="VYN16_000486"
     CDS             26095..27456
                     /locus_tag="VYN16_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1403064.1"
                     /translation="MNEKLAKSSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRRYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYMLNLFIKWYWKRAYPNFKGSKKVFLLTATSHVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATREVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIIGSLVGLILCG
                     LVSIVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMCVDAEDKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVVYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            27488..28666
                     /locus_tag="VYN16_000487"
     CDS             27488..28666
                     /locus_tag="VYN16_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1403065.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTEYQKDGNIQYYVACMR
                     ENSAKSGFTADTFEYNDAICYNIDVPNIGPARAIAYDIAAVNKAIEIAKKNKDEAPIF
                     YILACRIGPFIARLKKKIQAIGGTLFVNPDGHEWLRAKWSLPVRKYWKFSEQLMVKYA
                     DLLVCDSKNIEKYIQNDYKQYQPKTTYIAYGTDTSPSILKSEDLKIRSWYQEKGLSEN
                     GYYLVVGRFVPENNYETMIREFIKSKSKKDFVLITNVEQNKFYDQLLQETGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     DGAIYWRKDNLHKVIEESEQKTIEEIKEIDILSTEQVEKRFTWDFIVNEYENLFLLGK
                     "
BASE COUNT         8728 a         5151 c         6508 g         8659 t
ORIGIN      
        1 ttaaaaaaac tattgacaag ataaaaataa aggattacaa tggggaatat aaagtaaacc
       61 ggtaaaccta aacaagaaag gagaaaagat gaaaattgta cttgtagggc atggacattt
      121 tgctacaggg atttatagtt ctttacaatt gattgcaggt aatcaagaaa atgtggaggc
      181 gattgacttt gtggaaggaa tgtcagcaga tgaactcaag caaaaaatct tacttgcaat
      241 ttcaaatgaa gaagaagttt taatcctaag tgatctcttg ggaggatcgc cattcaaggt
      301 ttcttctacc ataatgggag aaaatccagc caaaacaatg aatgttctct cgggtttgaa
      361 cttagccatg ttaatggaag cagtctttgc tagaatggct catagctttg atgaggttgt
      421 taataaatca gtagtggtgg cccagggcgg agtcgtaaat ggtaaagaat tgttttcaac
      481 ggatgcagag gaagaggaag aagatttcga atcgggtatt taaagggtaa aagaatgata
      541 aaaaaggtta cgattgaaaa aataaaatcg cctgagcgct tcttagaagt accacttctg
      601 acgaaagaag aagtcggcca ggcaatcgat aaggttattc ggcagttaga actcaacctt
      661 gactatttca aggaagattt cccgacacca gctacctttg ataatgtcta tccaatcatg
      721 gataacacgg aatggaccaa tggtttctgg acaggagaac tgtggttggc ttatgaatac
      781 agtcaacagg atgcatttaa aaacatcgct cataaaaatg ttctttcttt cctggatcgt
      841 gtcaataaga gagtagaatt ggatcaccat gatctcggct tcttgtacac accgtcttgt
      901 atggctgaat ataagataaa tggagatgga gaggctagag aagcaacctt gaaagctgca
      961 gataagttga ttgaacgcta tcaagaaaaa ggtggtttta ttcaagcttg gggagacttg
     1021 ggcaagaaag agcattaccg tttgattatc gactgcttgc tcaatatcca actcttattc
     1081 tttgcttatc aagaaacagg cgatcaaaaa tactacgata ttgcagaaag tcatttctat
     1141 gcttcagcta ataatgtaat ccgtgatgac gcttcgtcct tccacacctt ctattttgat
     1201 cctgagacag gtcaaccctt taaaggtgta acgagacaag ggtatagtga tgattcatgc
     1261 tgggcacgtg gtcaatcatg gggagtctat ggtattcctt tgacttatcg tcacttaaaa
     1321 gacgagtcct gctttgactt gtttaagggt gtgaccaatt atttcttgaa tcgtctgcca
     1381 aaagatcatg tgtcctattg ggatttgatt tttaatgatg gtagtgctca atcacgagat
     1441 tcttcagcaa cagctatcgc cgtctgtggg attcatgaaa tgctaaaaca tctcccagag
     1501 gtggatgctg acaaagatat ttataaacat gctatgcatg ccatgcttcg ttccttgatc
     1561 gaacattatg caaatgatca atttacccct ggtgggacaa gtctcctcca cggtgtgtac
     1621 tcatggcatt caggtaaagg agtggatgaa ggcaatatct ggggtgacta ctattaccta
     1681 gaagccctta tccgtttcta caaagactgg aacctatatt ggtaggagga gaaatatgac
     1741 aatgccaaat attgttatga cccgtatcga tgaacggttg attcatggac aaggacaact
     1801 ttgggtaaaa tacctaggtt gtaatacggt cattgttgcc aatgacgaag taagcacgga
     1861 caagatgcaa caaactctga tgaaaacagt tgtgccagac tcagttgcca tgcgtttctt
     1921 ccctttgcaa aaggtgattg atatcattca caaggctaat cctgctcaaa cgatctttat
     1981 cgttgtaaag aatgtgaagg atgctttaac cttggtagaa ggtggtgtcc ctatcaaaga
     2041 aatcaatatt gggaacattc acaatgctac tggtaaagag caagtgacac gctccatctt
     2101 cctgggtgaa gaggacaagg tggccctcaa ggaattgagc caaactcatc aagtaacatt
     2161 taatacgaaa acaactccaa caggaaatga tggagctgtt caagtcaaca ttatggacta
     2221 tatttaaaag aggagatcgt tatgtcgatt aatgtatttc aagcgatttt aattggatta
     2281 tggacagctt tctgttttag tggaatgctg ttaggaattt acaccaatag atgtattgtt
     2341 ctgtcatttg gtgtcggaat tattctaggt gatctgccta ctgctcttgc aatgggagct
     2401 attggtgaat tggcttatat gggattcggt gttggtgctg gaggtactgt tccaccaaac
     2461 ccaatcggac ctggtatctt tggtaccttg atggctatca ctagtgctgg taaagtcagt
     2521 ccagaagcgg ctcttgccct ctctactccg attgctgtgg cgattcaatt cttacaaact
     2581 ttcgcctaca ctgtacgtgc tggtgcgcct gaaacggcga tgaagcactt gaaaaaccat
     2641 aatttgaaga aattcaagtt cactctaaat gcaacaattt ggttgtttgt ctttattgga
     2701 tttaccttgg gttgcttggg cgccctttca atggatacct tgttgaaact cgtagactac
     2761 attccaccgg tattgcttac aggtttgaca gttgctggta aaatgctccc agctatcggt
     2821 tttgcgatga tcttgtcagt gatggctaag aaagagttga ttccctttgt cttgttggga
     2881 tatgtttgtg cagcttatct aaacatccca acaattggta ttgcaattgt aggtactatc
     2941 tttgctttga ttgaatttta taacaagcca aaaacagcgg atcatgtggt agaggaggaa
     3001 gcacacgatg actggatcta acaaattaac aaaacgtgat tatcttaaaa cgtctttgcg
     3061 ggcattcttt tgtcaaaatg gatttaacta tagtaactat caagggttgg gatatgccaa
     3121 tgtgatgtat cctgctttga aaaaacacta tggagaggat caggaaggtt tctaccaagc
     3181 cttggaagaa aactgtgaat tctataatac caatccacac ttcctgcctt ttattaccag
     3241 cttgcacctt gtaatgttgg aaaatggccg accaacaaaa gaaacacgta gcatcaagat
     3301 ggccttgatg ggaccattag caggtattgg ggattctctc tctcaattct gtttagctcc
     3361 attgttctca accatcgcag cttcgtttgc tcaagaaggc ttggttgtcg gtccaatctt
     3421 gttcttcctt gcgatgaata cgattttaac agcgattaaa ttgtcaactg gtctgtatgg
     3481 atacaaacta ggaacaactg tgattgataa actaagcgaa cagatggcaa cgatttctcg
     3541 tattgccaat attatcggtg taaccgtaat tgctggtttg gcagcgacat ctgttaagat
     3601 tatggtgccg attacctttg ctgcagggga agttaaagca gacgctaaac aaagtatcgt
     3661 aagtattcag ggaatgcttg ataaggttgc tccagctctt ctaccagccc tatttacact
     3721 tttagtttac tacttgatca aagaaaagaa atggacaaca tataaactcg ttattttaac
     3781 agttatcatc ggaattatcg gaagctggct taagattata gcttaatgga tacaacattg
     3841 ttttatggaa tagtgattgt cttggcagtg agtccccttt tactgtcaag ctttcattct
     3901 attcgtcagc aaaagttgct tcgcaaacag atggagcaac gacaagagta tttagcttct
     3961 ttaacatctg gtgatgaagt gttgttattg tctggaattc atggaaaaat catttctatc
     4021 caagatgact tggtctcctt gcagattgca aaaggtgtgg tcatccatgt agaaaaggaa
     4081 agtgtaatgg gaaagacaaa agaactgctt tttaagtagt tctttttctg gtaatagata
     4141 atgaaagaag aaagaagaca attttttgaa agaatcgatg gaaaccaatg tcgtgattat
     4201 atcttgtcgc actgttcaaa agactatgag aaagtcaaga gttcccttga acgcttgatg
     4261 gacaatcgtt ttatgtttga tagtccttgg gatatggagc cttgttcaaa aatccatcaa
     4321 atccaaccga tggtatggga tcaagtattt gaagatgatc cagaatgggc ttatatgctc
     4381 aatcgacaag aatatctctt gcagtttatg atagggtatg tggtagaagg agataaggac
     4441 tatattcaaa agtgcaagtt ctttctattt gattggattg agcaggtgag agaattttct
     4501 cctcaatcct tgatgactag aaccttggat acgggtattc gttcctttac ttggttgaaa
     4561 ctactcttgc tcctcttgaa atttgacttg ctagaggaga aagaactaga gaaaattttg
     4621 gtcagtctag aaaagcagat tgactttatg aaaagccact atcgcgccaa gtacaccctt
     4681 agtaactggg gaattttaca aacaattccg atgcttgcta tctatcattt ctttgcagat
     4741 aagatggacc tagaagaagc ttaccatttt gcttcagagg agttgaaaca gcaaattgag
     4801 acacagattt taggagatgg aagccagttt gaacagtcga ttctctatca tgtagaggtt
     4861 tataaagcct tgctggattt gtgtctcttg cttccagact tgcaagatag ttaccaagag
     4921 ttgctggaaa agatggcgac ctatattcaa atgatgacag gcttagatgg acgaactttg
     4981 gcttttggtg atagcgattc tacagaaacg acagaaatgt tgagcctgtc tgctgtggtt
     5041 ttgaacaagg aagaccttct taacggtctg gatgttaaag tcgatttgct tagcctcttg
     5101 ttcctggggc gagaaaaggt caagcgactg caggaatttg aaaagagagc ttggcagcct
     5161 aggtccatga tctttgaaga ctctggacat gtctgcatta aggatgaaca tcgttatcta
     5221 tttttcaaaa atggtccgct aggaagtgcc catagccata gtgacgagaa tagtttttgc
     5281 ttacagtatc aaggccaacc tattttcata gatgctgggc gttattctta tcgggagata
     5341 tatgaacgtt atctcttaaa gagtgcttgg agtcattcga cctgcattgt agatgggaaa
     5401 gctccggaaa gaatcacggg atcctgggaa tatgaatact atcctcactc cctgttttgt
     5461 caccataaag aaagggaggg agtgcattat attgaagggg cttattggtc agcagaacct
     5521 gatttgcctt atcttcacaa gagaaaaatc ctcatgttgg tagaggatgt ctggctcttg
     5581 gtagatgaca tcaggtgtca aggtcagcat gaggcgttga ctcagtttat ccttgacaag
     5641 gatgtgacct atcaagatgg gaaaatcaat cagttgagac tatggagtga agttgatttt
     5701 gatttggaag ataccatcat ttctcctaaa tacaatgagc ttgaaagaag taacaaactc
     5761 accaagcgcc aattctttga gaatcagatg ctggattata ccatcattgc gcatgagagt
     5821 tttgaaatca tccgtcattc tgtctaccag acagatgatc gtgaagtgga aaatgctctg
     5881 gcttttgaag tgaaaaatga cgaaacagac aagctgattc tgttattaag cgaggatatt
     5941 tgtgtaggtg aaaaattgtg cctcgttgac ggaacaaaaa tgcgtggaaa atgtctagta
     6001 tatgataaaa taaatgagag aatgattcgc ttgcagtgct agaaataggc attttgaata
     6061 gtgaatatgt tataataagt attagtagga ggtgttttag attggagaag aaactgacca
     6121 taaaagacat tgcggaaatg gctcagacct cgaaaacaac cgtgtcattt tacctaaacg
     6181 ggaaatatga aaaaatgtcc caagagacac gtgaaaagat tgaaaaagtt attcatgaaa
     6241 caaattacaa accgagcatt gttgcgcgta gcttaaactc caaacgaaca aaattaatcg
     6301 gtgttttgat cggtgatatt accaacagtt tctcaaacca aattgttaag ggaattgagg
     6361 atatcgccag ccagaatggc taccaggtaa tgataggaaa tagtaattac agccaagaga
     6421 gtgaggaccg gtatattgaa agcatgcttc tcttgggagt agacggcttt attattcagc
     6481 cgacctctaa tttccgaaaa tattctcgta tcatcgatga gaaaaagaag aaaatggtct
     6541 tttttgatag tcagctctat gaacaccgga ctagctgggt taaaaccaat aactatgatg
     6601 ccgtttatga catgacccag tcctgtatcg aaaaaggtta tgagcatttt ctcttgatta
     6661 cagcggatac gagtcgtttg agtactcgga ttgagcgggc aagtggtttt gtggatgctt
     6721 taacagatgc taatatgcgt cacgccagtc taaccattga agataagcat acgaatttgg
     6781 aacaaattaa agaattttta caaaaagaaa tcgatcccga tgaaaaaact ctggtattta
     6841 tccctaactg ttgggcccta cctctagtct ttaccgttat caaagagttg aattataact
     6901 tgccgcaagt tgggttgatt ggttttgaca atacggagtg gacttgcttt tcttctccaa
     6961 gtgtttcgac gctggttcag ccctcctttg aggaaggaca acaggctaca aagattttga
     7021 ttgatcagat tgaaggccgc aatcaagaag aaaggcaaca agtcttggat tgtagtgtga
     7081 attggaaaga gtcgactttc taaaatgaag gaaaatgact tgcaatttct gttaagaaat
     7141 aaaataatcc cacctagaac aagctaggtg ggattatttg cctatgaaat gagaaattat
     7201 gggagcaagc tcctaaatca actgtttttg atctacttct ttaactactt gataaaagtt
     7261 atagaagtag gccaaacttg aaatgatggt tacgactagg aatattgaaa atttccattg
     7321 gacagggttg gttaaaagtt gtggaaagga tatgaggaga aagaagaggg ctgcgttgag
     7381 gacaggtatc cgttttgatt gtattttctc aagtccttta ttgagcgcag gaagaaagag
     7441 gagtaggagt agtaaaactg tatgagaaac agctcctgaa ataagggcaa agaaaaggaa
     7501 aatactgata aaaacatgaa tgatcagtag tctagctagt gatttcataa ggtacctcct
     7561 aatcctggtc ttttttagct cttgcaatac gaagtgagtc gacaatatgt atcatcactc
     7621 cgaaaaagaa agctcccagt atagttttaa aaatatgttt tgtatttaga agagaactga
     7681 taaaatttgg attttcactt gttagggtat caatgggtgg aattataaaa aatattattg
     7741 ttccataaat cgaacctgct ttcagaccag gataacgtaa ctgtttcttt tcttttttca
     7801 tagtttcctc ctaatcctca tccggatctt tcttagtttt tgcaatgcga cgggagatga
     7861 ggaactgtat gctcgctccg aagaagatag aaccgagaat gcttgataca ccatttctta
     7921 tagtgagaag agaatgaaaa tagtcctgac cttcacctat gagtataccg agaagaggag
     7981 ttataaaaaa catccataga ccaaagaaca aacctgcttt cagacctggg taatgtagtt
     8041 gcttactttc tttctcactc agcatatctg gatcaatagc tgtaatccct gcttttttca
     8101 tttggtaggt gacatagcca gcagcgatga gggcaatcac taaaatcaga ggaggataga
     8161 ttagagccac ttcttgaggg tatttatagg ccagaaggag tggaataaga tttccgaaaa
     8221 tcatcagata aaagaggacg ataaagactt ggttcccaat actatcggcc tcacgccgtt
     8281 tgtattcgtc aaggggacca gaaataccgt atgtgcgttt gatcagtttt tcagtaaagg
     8341 tttctttttt cataagtttg ctcctttttt aaaaatcttc ctcccaaaag agactgttga
     8401 ggtcagtttg gaggctgcgg gcgagattga gacagagttc cagagttgga ttgtacttgt
     8461 cgttttcaat catattgatg gtctgtctcg agacaccgat atccttggcg agttcgagct
     8521 gggaaatacc caattccttg cgaaattctt tcacacgatt catctggtct cctttctgaa
     8581 gtgtgtcgta tatatttgac tatattatat tcttttaaac ataaagtgtc aagtattttt
     8641 gacatatttt ttgaagaaat attagcgctc ttgtctgatt tgtctgacta gtgcaagctg
     8701 gtcagatttg tggtaaaata gataagatat gacaaaagaa tttcatcatg taacggtctt
     8761 actccacgaa acgattgata tgctcgacgt aaagcctgac ggtatctacg ttgatgcgac
     8821 tttgggtgga gcaggccata gcgagtattt attaagtaaa ttaagtgaaa aaggccatct
     8881 ctatgccttt gaccaggatc agaatgccat tgacaatgcg caacaacgct tggcacccta
     8941 cattgaaaag ggaatggtga cttttatcaa ggataacttc cgtcatttgc aggcacgttt
     9001 gcgcgaagct ggtgttcagg aaattgatgg catttgttat gacttgggag tgtctagtcc
     9061 tcaattggac cagcgtgaga gaggtttttc ttataagaag gatgcgccac tggacatgcg
     9121 catgaatcag gaagctagtc taacagccta tgaagtggtg aaccactatg actatcatga
     9181 cttgattcgt attttcttca agtatggaga ggacaaattc tctaaacaga ttgcgcgtaa
     9241 gattgagcaa gctcgagaag tcaaacctat cgagactacg actgaattgg cagaaattat
     9301 caagtcggcc aaacctgcca aggaacttaa gaagaagggt catcctgcca agcagatttt
     9361 ccaggctatt cgaattgaag tcaatgatga actgggagcg gcagatgagt ccatccagca
     9421 ggctatggag atgttggctc tggatggtag aatttcagtg attacctttc attccttgga
     9481 agaccgcttg accaagcaat tgttcaagga agcttcaacg gttgaagttc ccaaaggttt
     9541 gcctttcatc ccagatgatc tcaagcctaa gatggaattg gtgtcccgta agccaatctt
     9601 gccaagtgca gaagaattag aagccaataa ccgctcgcac tcagccaagt tgcgcgtggc
     9661 cagaaaaatt cacaagtaag aggaaaaaag atggcagaaa gaatggaaaa aacaggtcaa
     9721 atactacaga tgcaacttaa acggttttcg cgtgtggaaa aagcttttta cttttccatt
     9781 gctgtaacca ctcttattgt agccattagt attattttta tgcagaccaa gctcttgcaa
     9841 gtgcagaatg atttgacaaa aatcaatgcg cagatagaag aaaagaagac cgaattggac
     9901 gatgccaagc aagaggtcaa tgaactatta cgtgcagaac gtttgaaaga aattgccaat
     9961 tcacacgatt tgcaattaaa caatgaaaat attcgaatag cggagtaaga tatgaagtgg
    10021 acaaaaaaag taatccgtta tgcgaccaaa aatcggaaat cgccggctga aaacagacgc
    10081 agagttggaa aaagtctgag tttattatct gtctttgttt ttgccgtttt tttagtcaat
    10141 tttgcggtca ttattgggac gggcactcgc tttggaacgg atttagcgaa ggaagctaag
    10201 aaggttcatc aaaccacccg tacagttcct gccaaacgtg ggactattta tgaccgaaat
    10261 ggagtcccga ttgctgagga tgcgacatct tataatgtct atgcggtcat tgatgagaac
    10321 tataagtcag caacgggtaa gattctttac gtagaaaaaa cacaatttaa caaggttgca
    10381 gaggtctttc ataagtatct ggacatggaa gaatcctatg taagagagca actctcgcaa
    10441 cctaatctca agcaagtttc ctttggttcg aagggcaatg ggattaccta tgccaatatg
    10501 atggctatca aaaaagagtt ggaaactgca gaggtcaagg ggattgattt tacaaccagt
    10561 cccaatcgta gttacccaaa cggacaattt gcttctagtt ttatcggact agctcagctc
    10621 catgaaaatg aagatgggag caagagtttg ctgggaactt ctgggatgga gagttccttg
    10681 aacagtattc ttgcagggaa agacggtatt attacctatg aaaaagatcg tctgggtaat
    10741 attgtccccg gaacggaaca agtttcccaa caaacggtag atggcaagga tgtttataca
    10801 actatttcca gcacccttca gtccttcatg gagacccaga tgaatgcctt tcaagaaaaa
    10861 gtaaaaggca agtatatgac ggctaccttg gtcagtgcta aaacagggga aattcttgca
    10921 acaacgcaac ggccgacctt cgatgctgat actaaggagg ggctgactaa ggactttgtt
    10981 tggcgtgata tcctctatca aagtaactat gaaccagggt caaccatgaa ggtcatgact
    11041 ctcgctgctg ctattgataa taacactttc ccaggaggag aagttttcaa tagtagtgag
    11101 ttaaaagtag cagatgctac tatccgagat tgggacgtca atgaaggatt aactggcgga
    11161 ggaatgatga ccttttctca aggtttcgcg cactcaagta acgttgggat gacgcttctt
    11221 gagcaaaaga tgggagatgc tacctggctt gattatctca atcgctttaa atttggtatt
    11281 ccgactcgtt ttggcttgac ggatgaatat gcaggtcaac ttccagctga caatattgtc
    11341 aatatcgctc agagttcatt tggacaaggg atttcagtaa ctcaaacaca aatgattcgt
    11401 gcctttacag ccattgccaa tgatggcgtc atgttggagc ctaaatttat tacggccttg
    11461 tatgacccaa ataaccaatc tgttcgtaaa tctcaaaaag aaattgtagg gaatcctgtt
    11521 tctaaggatg cggcaagtca aactcggact catatggtct tagtaggaac ggatcctaga
    11581 tatggaacta tgtataatca tagtacagga aaagcaactg tcaatgttcc aggtcaaaat
    11641 gtagccctca agtctggtac agccgaaatc gctgacgaga aaaatggagg ctacttggtt
    11701 ggttctacca acaacatttt ctcagttgtg gctatgaacc ctgccgaaaa tcctgatttt
    11761 atcttgtatg tgacggtcca acaacctgaa cattattcag gtatccagtt gggagaattt
    11821 gccaatccta tcttggaaag agcagtggcc atgaaagatt cccttaacct ccaatctacc
    11881 gctaaaacgt tagatcaggt aaccaatcaa agcgcttatg ccatgcctag catcaaggat
    11941 atttcacccg gtgatttggc ggaagcctta cgtcgcaata ttgtgcaacc aatcgttgta
    12001 ggaacaggaa caaagattaa agaatcatct gtagaagaag gaagcaatct tgctcctaac
    12061 cagcaagttc ttctcttatc tgataaagca gaggaagttc cagatatgta tggttggaca
    12121 aaagcaaccg cagaagcttt ttctaagtgg ttaaatatag aacttgtatt tgaaggttcg
    12181 ggctctactg ttcagaagca agatgttcgt gctaatacag caatcaagga cattaaaaaa
    12241 attacattaa ctttaggaga ctaatatgtt tatttccatc agtgctggaa ttgtgacatt
    12301 tttactaact ttagtaggaa ttccggcctt tatcaaattt tatagaaagg cgcaaattac
    12361 aggccagcag atgcatgagg atgtcaaaca gcatcaggca aaagctggga ctcctacaat
    12421 gggaggtttg gttttcttaa ttgcttctgt tttggttgcc ttcttttttg ccctatttag
    12481 tagccaattc agcaataatg taggaatgat tttgttcatc ttggtccttt atggacttgt
    12541 cggattttta gatgactttc tcaaggtctt tcgtaaaatc aatgaggggc tgaatcctaa
    12601 gcaaaaattg gctcttcagc ttttaggtgg ggtcgttttc taccttttct atgagcgtgg
    12661 tggcgatatg ctttcagtct ttggttacca agtgcatcta gggattttct atattctttt
    12721 tgcccttttc tggctagtcg gtttttcaaa tgcagtcaac ttgacagacg gaattgatgg
    12781 tctggctagt atttctgttg tgattagttt gtctgcctat ggagttattg cctatgtgca
    12841 aggtcagatg gatatccttc tagtgattct tgccatgatt ggtggtctgc ttggtttctt
    12901 cgtctttaac cataagcctg ctaaggtctt tatgggtgat gtgggaagtt tggccctagg
    12961 tgggatgctg gcagctatct ctatggctct ccaccaagaa tggactctct tgattatcgg
    13021 aattgtgtat gtttttgaaa caacttctgt tatgatgcaa gtcagttatt tcaaactgac
    13081 aggtggtaaa cgtattttcc gtatgacgcc tgtacatcac cattttgagc ttgggggatt
    13141 gtctggtaaa ggaaatcctt ggagcgagtg gaaggttgac ttcttctttt ggggagtggg
    13201 acttctagca agtctcctga ccctcgcaat tttatatttg atgtaagaat ggcaccctga
    13261 tgtttcaggg tgtttttgtg tttaaataca caatgaaaat caaagaacaa actagaaagc
    13321 taactttagg ctgctcaaaa tatagtagat tgaaactaga atagtacaca tctacttcta
    13381 aaatattgtt agaaatcgat ttgactgtcc tgattgattt gtcctgttct tatttcgttt
    13441 taatatacag ttttgagatg gttgatagaa ctgacgaagt cagctcaaaa cacggttttg
    13501 aggttgtaga taaggagaag ctgatgtggt ttgaagaggt tttcgaagag tgtaaaaaaa
    13561 tattggtcag ttaaagtcaa aactcttgac cttttctgac caatgtagta tattatgaac
    13621 gtaaggtaaa tgaaagcctt actagaacac ttatttaaag taaaatagaa agaggtgggt
    13681 ctatgtttca tctagaaatc ttcagaagta aagatagtct actcctgctt gaaaaagaaa
    13741 aaccagaaat agtacgtagg gtagcgatct agctagtcta aattttttaa aacaatggtc
    13801 aaagatagtc aatatcagta atcataacta agtaaacaaa aagaggtaaa gaatatgaat
    13861 aacaacttta ataattttaa taacatggat gatttattta accaattgat gggtggtatg
    13921 cgaggataca gttctgaaaa tcgccgttac ttaattaatg gacgcgaagt cacacctgag
    13981 gaatttgctc actatcgtgc gactggtcaa ttaccaggaa atgcagaaac tgatgtgcaa
    14041 atgccacaac aggcatcagg tatgaaacaa gacggtgtcc ttgcaaaact aggtcgaaac
    14101 ttaacagcag aagcgcgtga gggcaagttg gatcctgtta tcggacgaaa caaggaaatt
    14161 caagaaacat ctgaaatcct ctcacgccgc accaagaaca atcctgtttt ggtcggagat
    14221 gcaggtgttg gtaagacagc agttgtcgaa ggtctagcgc aagccattgt gaacggagat
    14281 gttcctgctg ctatcaagaa caaggaaatt atttctattg atatctcagg tcttgaggct
    14341 ggtactcaat accgtggtag ctttgaagaa aatgtccaaa acttagtcaa tgaagtgaaa
    14401 gaagcaggga atattatcct cttctttgat gaaattcacc aaattcttgg tgctggtagc
    14461 actggtggag acagtggttc taaaggactt gcggatattc tcaagccagc tctctctcgt
    14521 ggagaattga cagtgattgg ggcaacaact caagacgaat accgtaacac catcttgaag
    14581 aatgctgctc ttgctcgtcg tttcaacgaa gtgaaggtca atgctccttc ggcagagaat
    14641 acttttaaaa ttcttcaagg aattcgtgac ctctatcaac aacaccacaa tgtcatcttg
    14701 ccagacgaag tcttgaaagc agcggtggat tattctgttc aatacattcc tcaacgtagc
    14761 ttgccagata aggctattga ccttgtcgat gtaacggctg ctcacttggc ggctcaacat
    14821 ccagtaacag atgtgcatgc tgttgaacga gaaatcgaaa cggaaaaaga caagcaagaa
    14881 aaagcagttg aagcagaaga ttttgaagca gctctaaact ataaaacacg cattgcagaa
    14941 ttggaaaaga aaatcgaaaa ccacacagaa gatatgaaag tgactgcaag tgtcaacgat
    15001 gtggctgaat ctgtggaacg aatgacaggt atcccagtat cgcaaatggg agcttcagat
    15061 atcgaacgtt tgaaagatat ggcccatcgc ttgcaagaca aggtgattgg tcaagataag
    15121 gccgtagaag ctgtagctcg tgctatccgt cgtaaccgtg ctggttttga tgaaggaaat
    15181 cgcccaatcg gcagcttcct ctttgtaggg tctactgggg ttggtaagac ggagcttgct
    15241 aagcaattgg cactcgatat gtttggaacc caggatgcga ttatccgttt ggatatgtct
    15301 gaatacagtg accgtacagc tgtttctaag ctaattggta caacagcagg ctatgtgggt
    15361 tatgatgaca atagcaatac cttaacagaa cgtgttcgtc gcaatccata ctctatcatt
    15421 ctcttggatg aaattgaaaa ggctgatcct caagttatta cccttctcct ccaagttcta
    15481 gatgatggtc gtttgacaga tggtcaagga aatacagtaa acttcaagaa cactgtcatt
    15541 attgcgacct caaatgctgg atttggctat gaagccaact tgacagaaga tgcggataaa
    15601 ccagaattga tggaccgttt gaaacccttc ttccgtccag aattcctcaa ccgctttaat
    15661 gcagtcatcg agttctcgca cttgactaag gaagaccttt ctaagattgt agatttgatg
    15721 ttggctgaag ttaaccaaac cttggctaag aaagacattg acttggtagt tagtcaagcg
    15781 gctaaagatt atatcacaga agaaggttac gacgaagtca tgggggttcg tcctctccgt
    15841 cgcgtggttg aacaagaaat tcgtgataag gtgacagact tccacttaga tcatttagat
    15901 gctaaacatc tggaagcaga tatggaagat ggcgttttgg ttattcgtga gaaagcctaa
    15961 gatagaattt tgaggataaa aaagaaggag ccagctgaaa aaaactggtt cctttttagg
    16021 tacgacaggc atgtcgtata gtagaagtgt attattccag tttcaatata ctatagtagc
    16081 tcagaagtcg gtacttaaac gtgctatatc aaaaccagtc cttgaaaaac gtggactggt
    16141 ttcgtgtttg gattattacc ttgaacgaca tgcgttaaaa gttagttgaa ccgccgtatg
    16201 ccgaacggca cgtacggtgg tgtgagaggg gctagagatt atcccctact cgattttaaa
    16261 tcacatgacg ttcaaaggca tcatctgaaa tcccttgttc caagatgagt tttgcccatt
    16321 ctttagcaga gaagaggctg tggtccttgt agtttccgca agattcgatg gttgtccctg
    16381 ggacatcttc ccaagtagta gtttcagcga tttccttgag cgaatccttg ataacagctg
    16441 caattttagc actggtgtga cgtccccaca taatcatgtg gaagcctgtg cggcaaccaa
    16501 atggcgaaca gtcaatcatg ccgtcaatgc gggtacggat gagtttggct aagaggtgct
    16561 cgatagtgtg aaggccggcg gtagggatgg agtcttcgtt tggttgcacc aagcgaatat
    16621 cataattgga gatgatgtct ccttttggtc ctgtttcttc cccaatcaag cgaacatagg
    16681 gtgctttgac aatggtgtgg tcaagttcaa aactttcgac aataacttct tttgacatgg
    16741 taaatccttt cagttttctt ctctcattat atcataaagg ttgctcctga gacagagaga
    16801 aaacctctcc gaggctggag aggttgaaat ctttacttac gatataagcg gtcgtattgg
    16861 tagtatgggt caaaggttac gttgataccc agtttacgaa ggacattctt gtcttcatca
    16921 gtcaagatga tggttgagtg ggcttcgctt cctttgaggt tgccgagttc ttccatagcg
    16981 cgggcagcat caggattttc tgtagctgtg atagcaagtg caatcaggat ttcatttgaa
    17041 tgaaggcgtg gattgcggct accgagatga tcgattttaa gaccttggat tggcttaaca
    17101 acttccggct cgattaattt tacttcttta gcgatgtcag ctgatttttt gatggcgttg
    17161 atcaaggcag cggctgtagg cccaaagagt tctgagttct taccagtgac gatttcccca
    17221 tttggcaatt caaaggctag ggctggtcca ccagtttctt ctgctttttg gcgcgcaacg
    17281 acagcaacct tacggtctgc aggtgtgata ccgaggtcgt tcatgagcaa ctcaattttc
    17341 ttgacggcag cttcgccaac tttttcagct ttgaagtcaa gaactgtttg atagtaacgg
    17401 cggatgattt cttgtttaga agcttcgaca gcagcctcgt catctgtaat agcgaaacca
    17461 accatgttga cacccatatc tgtcggtgaa gcgtatggtg attttccgag aatacgttcc
    17521 aacatgcgct tgagcactgg gaagatttcg atatcacggt tgtagttgac agtggtttct
    17581 ccataggttt gaagatggaa ggggtcaatc atgttgacat catcaaggtc agctgtggca
    17641 gcttcataag ccaagttaac tggatgatga aggggaagat tccaaacagg gaaggtttca
    17701 aatttagcgt agccagattt gatgccattg atttggtcgt gatacatatt ggacatacac
    17761 gttgccaatt ttccagaacc aggtccagga gcggttacga caatcaagtt gcgactggtt
    17821 ttgatgtagt cgtttttgcc catgccttct ggggaaatga tgtgatccat atccgtcgga
    17881 tatcctttga ttggataatg aagataagaa tcaattccgt ttttctcaag ttggttgcgg
    17941 aaggcatctg cagcgggttg gccagcgtat tgtgtaatga caacggaacc aacaaaaatc
    18001 cctaattcat tgaatttgtc aatcaaacga agaacttctt ggtcataaga aatgcctaag
    18061 tcgccacgtg ctttggaatg ttcaatgttg ctagcattaa tggcaatcac aacctcaacc
    18121 tgctctttca attcttgcaa gagcttgatt ttgttgtcag gttcataacc aggaaggaca
    18181 cgagcagcgt ggaaatcttc taacatttta ccgccaaact ctaagtagag cttgccgtca
    18241 aattggttaa tgcgctccaa aatatggtcg cgttgtaaat tcaaatattg ttcagaacta
    18301 aaagcttgtt ttttcatttt tttacctctg gactctatta taataaaaaa ttggaagtta
    18361 ggaaactacg gagctaaaaa agaaattaaa aagattaagc aaacgcttgc acaaaatttt
    18421 aaaaagtgct atcatagact atagattatg aaaataatga ggtaaacaga tgcaagaaaa
    18481 atggtggcat aatgccgtag tctatcaagt ctatccaaag agttttatgg atagtaatgg
    18541 agatggaatt ggcgatttgc caggaattac cagtaagttg gactatctag ccaagctagg
    18601 aatcacagca atttggcttt ctcccgttta tgacagccct atggatgata atggctacga
    18661 tattgctgat tatcaagcga ttgcaacgat ttttggaacc atggaggata tggatcaact
    18721 gattgcagaa gctaagaagc gtgatatccg tatcatcatg gacttggtgg tcaatcatac
    18781 ctcggatgag catgcctggt ttgtagaggc ctgtgaaaat cctaatagcc ctgagcgaga
    18841 ctactatatc tggcgcgatg aacccaatga cctagattct atctttagtg ggtctgcttg
    18901 ggaatacgat gaaaagtcag gtcaatacta tctccacttt ttcagcaaga aacagccgga
    18961 tctcaactgg gaaaatgaaa aacttcgcca gaaaatttat gagatgatga acttctggat
    19021 tgataaaggt attggtggtt tccgtatgga tgttattgac atgattggca aaattcctga
    19081 cgagaaggta gtcaataatg gtcctatgct ccatccctat ctcaaggaaa tgaatcaggc
    19141 gacctttgga gataaggatt tcttgacagt aggggagact tggggagcaa cgccagagat
    19201 tgccaagctc tactctgatc caaaggggca agaattgtct atggtcttcc agtttgaaca
    19261 tatcggtctt cagtatcagg aaggtcagcc taaatggcac tatcaaaaag agctgaatat
    19321 cgctaagtta aaagaaatct tcaacaaatg gcagacagag ttaggagttg aggacggctg
    19381 gaattccctc ttctggaaca accatgacct ccctcgtatt gtctcaatct ggggaaatga
    19441 ccaagaatac cgcgaaaaat ctgccaaagc ctttgcaatc ttacttcatc tcatgagagg
    19501 aactccttat atctaccaag gtgaggagat tgggatgacc aactatccgt ttgaaacact
    19561 ggctcaagta gaagatattg aatctctcaa ctatgcgcgt gaggctcttg aaaaaggtgt
    19621 tccgattgaa gaaatcatgg acagtatccg tgttattgga cgtgacaatg cccgtacccc
    19681 tatgcaatgg gacgagagca aaaacgctgg tttctcaaca ggtcaacctt ggttggcggt
    19741 taatccaaac tacgaagaaa tcaacgttca agaagcactg gcaaactcag attctatttt
    19801 ctatacctat cagaaactca tccagattcg taaggaaaac agctggttga ttcgagctga
    19861 ttttaaatta ctcgatacgg ctgataaggt ctttgcttat atccgtaagg acggtgaccg
    19921 tcgcttccta gtcgtggcta atttatccaa tgataaacaa aacttttcag tagatggaaa
    19981 agttagatct gtcttgattg aaaacactgc ggctaaagaa gtacttgaaa aacaggtctt
    20041 gactccatgg gatgctttct gtgtggaaat gactgactag aatgagcaag agcctgggac
    20101 aaaatagttc tcgaccacaa aaaagctaga gatttccaat tgtggaactc tagcttttta
    20161 attttgagtc attctcttat tgtattttag gaggttattg gccataagta ccaatcccat
    20221 gtcaattctc acttgacgct tgcccctcag attacatctc ttgtaaccca aacaagcctt
    20281 tatctgccca aagacaggtt ccacatcaat cttgcgttga gcgaaaatct gtctaccttg
    20341 gggagataaa agcgcctgac attctttagc tttcaagttt tgatagcctt cgttcatata
    20401 cagtcccctt ttgaggggct gattcaggtt cgtcgacgta gtaaaccttg atttcttgtt
    20461 gaaagtctgt ctgtgttttc tgatgtttgg tatggtgaaa acgataatac tagccatcag
    20521 gatgtgtata gctatcctcc ttgtcattat agtgccaatt cgctaagttt ctagctgact
    20581 gtttatagcc tctcttctgt tccttatcaa acatggcata tttaatcaga tggtttacct
    20641 ccttttcatc taaacgaagg aggttctctt cacttccata tccagcatct gcgacaactg
    20701 tcttcaagtc atgcggatag gtttcaagga atggcagaag agtcttggta tctgtcggat
    20761 ttgaaaagac atcatagtga agaacaaatt ggttttccgt agcgatttga agattataag
    20821 cagccttggg ttgactattt ttcatatgtt cttccttcat ccgcataaaa gtggcatctg
    20881 gatcggtttt ggaaaaactg ttaggccctt gaaatgtctc ctgatagttc tcatattttt
    20941 cagcacgtac tgaaaaatcc tcctttaaac tcttcataat cctttttatt tttatgaaga
    21001 tattgtttga aagatgtgag tttccacgga tgggtttgtg gagggatata cttgcgtctt
    21061 tctttttttg ttctggttct tgttcaaggt ttttcgaata gagttcatga tttagtagct
    21121 cctttgtgtg atagattttg tcagcgatat tgaggtagat gtcaccatca aatgctttta
    21181 taactaatgc ttttgtcttt ctgatgaaat agacttcttt tccttgctcg gtagggatat
    21241 agcaacgatt ttggaatcgg atatggtgtc cactatcgac gactctctcc cgccagttta
    21301 gctagaatga gatttcgttc agagggctta ggagcctcct caaagacaga gagttttgtc
    21361 ttgtttccaa actgttcatt aaaggtttgg atataggaag gcagaaaggt attggcttct
    21421 tccaaggtat gaatcttgtt tcgttccagt tcgataggca ggagagattg tagtatctga
    21481 ttgagtcttt cgactctccc tttagcttga gggatagagg ttgtctcaag gagaattccc
    21541 agttggtgac aggcgtatcc aaattgtgta tgggtgtcgt cctccatttt cttagagttg
    21601 gaggcttgat aggtaaaaac agttctctta tctgttttga tttgaacagg aatgccgtgg
    21661 ttggctaaga tttgttcgag gacatgatag taagcattca aggtctcttg tttgtcaaaa
    21721 taagcgccta ggatattgcc agaagcatca tcaatggcta ggtgtaagtt agaggtttct
    21781 gctccaaacc aggcatgagg gctggcatcc atttggatga gttctccagc aaatttcttt
    21841 ctgggtctac taggatgtac ctttttaggg tcttccagga agttttcagc cgtcggtaag
    21901 attggattgt ctaggggttg cttggggttc agtttagctt gctttcttac tctcttcttt
    21961 gtctttctat gggacttagg cgacaggata tttttcttat agagtatttt tctaacagtt
    22021 gtatcagaga gctgaattcc ttcttcttca gctagcaatt cacagaaatg acggacattt
    22081 ggtttatatg tttcatagga gaggtatttc tttaggatac gttctttgat ttcatcaggg
    22141 attgcatgtt ttggttttcg atttctgttt ccgtgtctga aggcttcttt tcctttctgt
    22201 tgataggcta gtagcagacg attgatttgc ctttcagaaa gattgagctc gacacaggct
    22261 cgtttctttg ttttctttcc ttgggctata gcttttatca caagatattt tttcgtttca
    22321 ttcatattca gttggatcct ttacatatga ctattctacc aaatgggaca tttttacgtt
    22381 cgatttacta aagacattat cacattcgaa ttacacaaga tgcagatagt gaaaaaaagg
    22441 tgtagacatt accgtaaaaa agtgatataa ttgtaagatg ttcaatgtat aggtgttaat
    22501 catgagtaga cgttttaaaa aatcaggttc acagaaagtg aagcgaagtg ttaatatagt
    22561 tttgttgact atttatttat tgttagtttg ttttttattg ttcttaatct ttaagtacaa
    22621 tatccttgct tttagatatt ttaatctagt ggtaactgcg ttagtcctac tagttgcctt
    22681 ggtagggcta ctcttgatta tccataaaaa agctgcaaag tttactattt ttctgttggt
    22741 gctctctatt cttgtcagct ctgtgtcgct ctttgcagta cagcagtttg tgggactgac
    22801 caatcgttta aatgcgactt ctaattactc agaatattcg atcagtgtcg ctgttttagc
    22861 agatagtgag attgagaatg ttacgcaact gacgagtgtg acagcaccga ctgggactga
    22921 taatgaaaac attcaaaaac tactagctga tatcaaatca agtcagaata tcgatttgac
    22981 ggtcaatcaa agttcgtctt acttggcaac ttacaagagt ttgattgcag gagagactaa
    23041 ggccattgtc ttaaatagtg tctttgaaaa tatcatcgaa tcggagtatc cagattacgc
    23101 atcgaagata aaaaagattt ataccaaggg attcactaaa aaagtagaag ctcctaagac
    23161 gtctaagaat cagtctttca atatctatgt tagtggaatt gatacttatg gtccgattag
    23221 ttcggtgtcg cgttcagatg tcaatatcct gatgactgtc aatcgagata ccaagaaaat
    23281 tctcttgacc acaacgccac gtgatgctta tgtaccaatc gcagatggtg ggaataatca
    23341 aaaagataaa ttgacccatg cgggcatcta tggagttgat tcgtccattc acaccttaga
    23401 aaatctctat ggagtggata tccattacta tgtccgtttg aacttcactt ctttcttgaa
    23461 attgatcgat ttgttgggtg gggtagatgt ttataatgac caagatttca catctctaca
    23521 cgggaagttc catttcccag ttggaaatgt ccacttagat tctgagcagg ctctgggatt
    23581 tgttcgtgag cgctattccc tagcggatgg tgatcatgac cgcgggcgca atcaacaaaa
    23641 ggtgattgcg gctatccttc aaaaattaac ttcgagcgaa gcgctgaaaa attatagtat
    23701 gatcattgat agcttgcaag attctatcca aacaaacatg ccccttgaga ccatgataaa
    23761 cttggtcaat gctcagttag aaagtggtgg aacgtacaaa gtaaattcgc aagacttgaa
    23821 gggtagggga cggacggatc ttccttccta tgcgatgcca gatagtaacc tctatatgat
    23881 ggaaattaac gacagtagcc ttgcatctgt caaaactgct attcaggatg tgttggaggg
    23941 cagatgaaat gattgatatt cattcgcaca ttgtctttga tgtagatgat ggtcccaagt
    24001 caagagagga aagtaaggct ctcttgacag aagcctacag gcagggggtg cgaaccattg
    24061 tctctacctc tcaccgtcgc aagggcatgt ttgaaactcc agaagagaag atagcagaaa
    24121 actttcttca ggttcgggaa atagctaagg aagtcgcgag tgacttggtc attgcttatg
    24181 gggccgaaat ttactacaca ccagatgttt tgggtaagct ggaaaaaaat cggattccga
    24241 ccctcaataa tagtcgttat gccttgatag agtttagtat gaacacccct tatcgcgata
    24301 ttcatagtgc cttgattaaa atattgatgt tgggaattac tccagtcatt gcccacatcg
    24361 agcgctatga tgctcttgaa aataatgaaa aacgcgttcg agagctgatt aatatgggct
    24421 gttacacgca agtaaatagt tcacatgtcc tcaaatccaa actttttgga gaaccttata
    24481 aattcatgaa aaaaagagct cagtatttct tggagcgtga tttggttcat gtgattgcaa
    24541 gtgatatgca taatgtggac agcagacctc cccatatggc agaagcatat gaccttgttt
    24601 cccaaaaata cggagaaacg aaggctcagg acctttttat agacaatcct cgaaaaattg
    24661 taatggatca actaatttag gagaaataat gaaagaacaa aacacgatag aaatcgatgt
    24721 atttcaatta cttaaaacct tgtggaaaaa caagctaatc attttattag tggcacttgt
    24781 gacaggggcg ggagcttttg catatagcat ttttattgtt aagccagaat atacaagcac
    24841 cacgcgtatt tacgtagtca atcgtaatca agaaaataag ccgggactga cgaatcagga
    24901 cttgcaggca ggaacttatc tggtaaaaga ctaccacgaa attatccttt cgcaagatgt
    24961 attggaaaag gtagcgacaa atttgaaatt ggatattcca gtaaaaacgt taactagcaa
    25021 agtgcaagtg actgtaccag ccgacactcg tatcgtctca atctctgtca aggataaaca
    25081 accagaggaa gccagtcgca ttgctaattc tatacgagaa gttgctgcag aaaagatcat
    25141 cgctgtaacg cgagtatctg atgtaacgac gcttgaagaa gcgcgaccag ctacgactcc
    25201 ctcttctcca aatgttcgac gcaacacctt agttggtttt cttggagccg ccgccgtaac
    25261 agtaattact gttcttttga ttgagttgtt cgatacccgt gtaaaacgtc ctgaagaagt
    25321 tgaagatgta ctgcaaatgc cacttctagg agtcgttcca gattttaata aaatgaaata
    25381 ggaggaagtt atgccaacgt tagaaatctc acaggcgaaa ttagaacttg cgaaaaagac
    25441 agaggaatat tataatgctt tgtgcacgaa cctacagtta agtggagatg atttgaaagt
    25501 attttctatc agttctgtga aagcaggaga aggaaaaaca acgacttcca ccaatatcgc
    25561 ttgggctttt gcgcatgcag gttacaaaac gctgctgatt gatgcagaca tgcgtaactc
    25621 cgttatgtca ggtgtcttta aatcaaggga aaggattact gggctaacag aatttctatc
    25681 agggacaaca gacctgtcac aggggctttg cgataccaat gttgagaatc tctttgtgat
    25741 tcaggctggc tctgtgtcac caaatccgat agcccttctt caaagtaaga atttcagtac
    25801 aatgcttgga accttgcgta aatattttga ctatatcgtc gtagacactg ctcctatcgg
    25861 tatcgtgatt gatgcggcta tcattatgca gaaatgtgat gcttctattt tagtgacgaa
    25921 ggcaggtgaa acaaaacgac gggaacttca aaaagcgaaa gaacagttgg aacaaactgg
    25981 gaagtcgtgt ttaggagttg tgttgaataa attcgatact tcagtagaca aatacggttt
    26041 ttatgggagt tatgggagtt acaggaaaca gaaaaaatag gttgagggat agagatgaat
    26101 gaaaaactag caaagtcttc agtagctata gtccagagtt ttcttgttat tttattgact
    26161 tatctactta gcgctgtgag agaaacagag attgtttcaa caacagctat tgtactttat
    26221 atcctccact attttgtctt ttatatcagt gattatggac ggaatttctt taaaaggaga
    26281 tatttgattg aacttgtcca gacattgaaa tatatcctat tctttgcgct agcgattagt
    26341 atttctaatt ttttcttaga ggatcgattt agtatttcca gacgaggcat gatttacttc
    26401 ctcctattac atgttttctt agtctatatg ctaaacctat ttatcaagtg gtattggaag
    26461 cgggcttatc ccaactttaa aggaagtaag aaggttttcc tacttacagc aacttctcat
    26521 gtcgaaaagg tactggatag actaatagaa tcagatgatg ttgttgggga gttggtagcc
    26581 gtcagtgttt tagataaacc agattttcag catgatgatt taaaggttgt agcagagggg
    26641 gagatcgtaa actttgcgac tcgtgaggtg gtcgatgaag tctttatcaa tcttccaagt
    26701 gaaaaataca atattggaga gcttgtctct cagtttgaaa cgatgggaat tgatgtaaca
    26761 gtcaatctaa atgctttcga ttgggcacgt aacaagcaaa tttgtgagat ggcaggacta
    26821 aatgttgtga ctttttctac aacattttat aagactagcc atgtgattgc taagcggatt
    26881 attgatatta tcggttccct ggtaggtttg atactatgtg gtctagtcag tattgtgctg
    26941 gttcctttga ttcgaaagga tgggggctct gctatttttg ctcagacgcg tataggaaaa
    27001 aatggccgcc atttcacttt ttacaagttt cgctctatgt gtgtagatgc tgaggataaa
    27061 aagagagaac tcatggaaca aaataccatg cagggtggaa tgtttaaggt ggacgatgat
    27121 cctcgtatca cgaaaattgg tcattttata cggaagacga gcttggacga gctaccacag
    27181 ttttataatg ttctaaaggg agatatgagt ttggtaggta cacgaccacc aacagtggac
    27241 gagtatgaac actatacccc agaacaaaaa cgtcggctaa gttttaaacc tggtataaca
    27301 ggcttatggc aggtcagtgg acgaagtgaa atcaaaaatt tcgatgaagt tgtcaaatta
    27361 gatgtggtct atattgatgg ttggacaatc tggaaagata ttgaaatttt attgaagaca
    27421 gttaaagttg tattgatgaa ggatggagcg aaatagtttg cttttgtttc agactattag
    27481 gagaaaaatg aagaagtcag tttatatcat tggttcaaaa gggattcccg ctaagtatgg
    27541 gggctttgaa acctttgttg agaaattaac agaatatcaa aaagatggta acatccaata
    27601 ctatgttgcc tgcatgcgcg aaaattctgc aaaatcagga tttacagcag atacatttga
    27661 gtacaatgat gctatttgtt acaacattga tgtgcctaat attggtcctg ctagagccat
    27721 tgcttatgat attgctgctg tgaataaagc gattgaaata gctaagaaaa acaaagatga
    27781 agctcctatt ttttacattc tagcttgtcg tatcgggcct tttatagcta gacttaagaa
    27841 aaagattcaa gctatcggag gtactctgtt tgtcaatcca gatggacatg agtggcttcg
    27901 ggctaaatgg agcctgccgg ttcgaaagta ttggaaattt tcggaacagt tgatggtcaa
    27961 atatgcagat ttattggtct gtgatagcaa aaatattgaa aaatatatcc agaatgacta
    28021 taagcaatat caaccaaaga cgacttatat tgcttacggg acggatacaa gtccgtctat
    28081 cctgaaatcc gaagatctaa aaattcgtag ttggtatcag gaaaaaggac ttagcgaaaa
    28141 tggctattat ctagtggtgg gacgattcgt ccctgaaaac aactacgaaa ccatgattcg
    28201 tgaatttatc aaatctaagt ccaagaagga ctttgtcctc atcacaaatg tggagcagaa
    28261 taaattttac gaccagttgt tacaggagac tggttttgac aaagatccta gggttaaatt
    28321 tgtaggtact gtctatgacc aagaattgct taagtacatt cgtgaaaatg cttttgccta
    28381 tttccatggg catgaggttg gagggaccaa cccttctcta ctagaagctc tagcatccac
    28441 aaaactgaat ttgttgctag atgttggttt taaccgtgag gttggtgaag acggggctat
    28501 ttattggaga aaagataatc ttcataaggt tattgaagag agtgaacaaa aaacaataga
    28561 agaaataaaa gaaatagata ttctatctac agagcaggta gaaaaaagat ttacttggga
    28621 ttttattgtt aatgaatatg aaaatttatt tttactaggg aaataattat ctaagttaca
    28681 aggattttat ttgaggaagc tttgatggaa gttcatcact ttcttagaca attatctaaa
    28741 taacttatgt tattcatgaa gtttttaaaa tgatagatat tctccctttt tgcaaagata
    28801 ctaaaattat agagaggaaa tatgcccagt agttgatagt gtttttgggt agggttaaac
    28861 gacatagttt taagacaaac tggtggagat ttaagaaata agtagttctt attaaatcaa
    28921 aaacgtgttc ttattctata ttgtcattta gtcaaaggta tcaagtatga ctattaaaat
    28981 taattatatg ttttttgttt gtctctcttt tttcggtata gttttaagta gttcacaggt
    29041 aatagt
//
LOCUS       DAVLTM010000013        28148 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLTM010000013 DAVLTM010000000
VERSION     DAVLTM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.013"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..191
                     /locus_tag="VYN16_000488"
     CDS             <1..191
                     /locus_tag="VYN16_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1403066.1"
                     /translation="WGLLKLGVPKWIADKVSGWGDHYQLVAQKSVLKRAISKPVLEKR
                     GLVSCLDYYLERHALKVS"
     gene            648..1826
                     /locus_tag="VYN16_000489"
     CDS             648..1826
                     /locus_tag="VYN16_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1403067.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2140..2271
                     /locus_tag="VYN16_000490"
     CDS             2140..2271
                     /locus_tag="VYN16_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1403068.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2292..3842
                     /gene="dltA"
                     /locus_tag="VYN16_000491"
     CDS             2292..3842
                     /gene="dltA"
                     /locus_tag="VYN16_000491"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1403069.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMSGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3839..5083
                     /gene="dltB"
                     /locus_tag="VYN16_000492"
     CDS             3839..5083
                     /gene="dltB"
                     /locus_tag="VYN16_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1403070.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5097..5336
                     /gene="dltC"
                     /locus_tag="VYN16_000493"
     CDS             5097..5336
                     /gene="dltC"
                     /locus_tag="VYN16_000493"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1403071.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5329..6597
                     /gene="dltD"
                     /locus_tag="VYN16_000494"
     CDS             5329..6597
                     /gene="dltD"
                     /locus_tag="VYN16_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1403072.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6752..7192
                     /gene="adcR"
                     /locus_tag="VYN16_000495"
     CDS             6752..7192
                     /gene="adcR"
                     /locus_tag="VYN16_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1403073.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7192..7896
                     /locus_tag="VYN16_000496"
     CDS             7192..7896
                     /locus_tag="VYN16_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1403074.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7889..8695
                     /locus_tag="VYN16_000497"
     CDS             7889..8695
                     /locus_tag="VYN16_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1403075.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8705..10210
                     /gene="adcA"
                     /locus_tag="VYN16_000498"
     CDS             8705..10210
                     /gene="adcA"
                     /locus_tag="VYN16_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1403076.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11337..12110)
                     /locus_tag="VYN16_000499"
     CDS             complement(11337..12110)
                     /locus_tag="VYN16_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1403077.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            12286..13689
                     /locus_tag="VYN16_000500"
     CDS             12286..13689
                     /locus_tag="VYN16_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1403078.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSTLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLVYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13804..14442
                     /locus_tag="VYN16_000501"
     CDS             13804..14442
                     /locus_tag="VYN16_000501"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1403079.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14454..14894
                     /locus_tag="VYN16_000502"
     CDS             14454..14894
                     /locus_tag="VYN16_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1403080.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14887..15318
                     /locus_tag="VYN16_000503"
     CDS             14887..15318
                     /locus_tag="VYN16_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403081.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15309..15779
                     /locus_tag="VYN16_000504"
     CDS             15309..15779
                     /locus_tag="VYN16_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1403082.1"
                     /translation="MPIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15804..16577
                     /locus_tag="VYN16_000505"
     CDS             15804..16577
                     /locus_tag="VYN16_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1403083.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16574..17374
                     /locus_tag="VYN16_000506"
     CDS             16574..17374
                     /locus_tag="VYN16_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1403084.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17433..19727
                     /locus_tag="VYN16_000507"
     CDS             17433..19727
                     /locus_tag="VYN16_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1403085.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19737..22853
                     /locus_tag="VYN16_000508"
     CDS             19737..22853
                     /locus_tag="VYN16_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1403086.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            22910..24676
                     /locus_tag="VYN16_000509"
     CDS             22910..24676
                     /locus_tag="VYN16_000509"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1403087.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            24822..25973
                     /locus_tag="VYN16_000510"
     CDS             24822..25973
                     /locus_tag="VYN16_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1403088.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMVTNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            26117..27001
                     /locus_tag="VYN16_000511"
     CDS             26117..27001
                     /locus_tag="VYN16_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1403089.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(27119..27196)
                     /locus_tag="VYN16_000512"
                     /pseudo
     CDS             complement(27119..27196)
                     /locus_tag="VYN16_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            27400..27652
                     /locus_tag="VYN16_000513"
                     /pseudo
     CDS             27400..27652
                     /locus_tag="VYN16_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            27640..27930
                     /locus_tag="VYN16_000514"
     CDS             27640..27930
                     /locus_tag="VYN16_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403090.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT         8861 a         4600 c         5982 g         8705 t
ORIGIN      
        1 tatggggatt gcttaagtta ggagttccta aatggatagc agataaggta tctggctggg
       61 gcgaccatta tcaattagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag
      121 tccttgaaaa acgtggactg gtttcgtgtt tggattatta ccttgaacga catgcgttaa
      181 aagttagttg aaccgccgta tgccgaacgg catgtacggt ggtgtgagag gggctagaga
      241 ttatccccta ctcgattaac tcccctgaaa tttattttaa ttatgcaaat ttcacgtatt
      301 tttgatgctg agacgacgat ctgggaactt ttcagatatt tttttgacta tctaaaatct
      361 atcattagaa aagcttagag cgccaaagga tttgagcgtt tttctgattt ttaagacttt
      421 ttcccagtct ctttttcgat tgaagatgta attattctac taactaacta acttcttagt
      481 actagccaac aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt
      541 tttgtttttt catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta
      601 aaaatcaaat ttaattccaa agtttgtaac taaaggggga gcgctacatg tctaattcat
      661 ttgtcaagtt gttagtctct caattatttg caaatttagc agatattttc tttagagtaa
      721 caatcattgc taacatatac attatttcaa aatcagtaat tgccacatca ctagttccta
      781 tcttaatagg aatatcctct tttgttgcga gtcttttagt tccgttggtt actaaaaggt
      841 tagcgctaaa tagggtttta tctttatctc aatttggaaa gactatatta ttggcgatac
      901 tggtaggaat gtttaccgta atgcaatccg tagcgccttt ggtgacctat ctatttgttg
      961 ttgcaatttc catactagat ggttttgcag cacccgtttc ctatgctatt gtgccacgct
     1021 atgcgaccga tttgggtaag gctaattcag ccttatcaat gactggtgaa gctgttcaat
     1081 tgataggttg gggattaggt ggactcttat ttgcaacaat tggtctgtta cctaccacgt
     1141 ttatcatttt agtcttgtat atcatttcta gctttctgat gttatttctt cctaacgctg
     1201 aagtggaggt gttagagtca gaaactaatc ttgaaatttt gctcaaaggt tggaagttag
     1261 ttgctagaaa tcctagatta agactttttg tatcagcaaa tttattggaa attttttcaa
     1321 atacgatttg ggtttcttcc attatacttg tttttgtaac ggagttatta aataaaacgg
     1381 aaagttactg gggatattct aatacagcat actctattgg tattataatt agtggcttaa
     1441 ttgcttttag gctatctgaa aagttccttg ctgctaaatg ggaaagtatt ctttttcctc
     1501 ttgtagctat ggcaatagtg acactgacta ttctatattt tccaaacgca cagatgtttt
     1561 tattattttc agctttagtt ggtatgttat cgcaactaaa agaagttcct gaaagtgtat
     1621 ttcttcagga aacagtagag gaaaatcatt tagttaatgt ctattccgtt cttgaagtga
     1681 tttctacatt agcattttca gtatttgttt tgctaatgag ctatattact gagagttttg
     1741 gtattagcat cagtttttgg ctatcagcca tttgtctgat gatagaagct attttaattt
     1801 atatcagacg agattatttt aaatgagaga gtggttaaaa agtctttaaa aatcagaaaa
     1861 gcgcatagta tcaggtgttg aaaaaacttg atattgtgtg ttttattatg ggaagattta
     1921 cttcattgtc tcctgaaatg gagttttgaa gcaggatcac tctatttgaa aatatacata
     1981 gaaaaactgt ataatcctta aaataagctg aaatctattg gaaacatacg gtaaacaatt
     2041 gaaaagatac tatataaagt ggtacaatgg tagaaaaata agctagtaag aatcactctt
     2101 attttagcga aaaattacag aaatgaatgg tttttcttga tgaaacagag aaaagaattg
     2161 tacctctttc ttggtcggac agccttgtat tttcttatct ttctagggct gctttacttc
     2221 tttagctatc ttggtcaggg tcaaggaagc tttatctata atgaatttta acttgaagga
     2281 gaatccctat tgtgtcaaat aaaccaatag cagatatgat tgaaaccatt gagcattttg
     2341 ctcagacaca gcctagctat cctgtctata atgttttggg gcaggaacac acttatggcg
     2401 atttaaaggc tgattcggat agtttggctg cagtcattga ccaactaggc ttgcctgaga
     2461 agtctcctgt ggttgttttt ggtggccaag aatatgaaat gttggcaacc tttgtagcgc
     2521 tgactaagtc aggtcatgcc tacattccaa ttgatagcca ttcggccttg gagcgagttt
     2581 cagctatttt agaagtagca gagccaagct tgattattgc catttcagcc tttcccttgg
     2641 agcaggtttc tacaccaatg ataaatctag ctcaggttca agaagccttt gcccaaggga
     2701 acaactatga aatcacgcat ccagtcaagg gagatgataa ttactacatt atctttactt
     2761 ctggtacgac tggtaagcct aagggagtgc agatttcaca tgataatctc ctcagcttta
     2821 caaactggat gattacggat aaggaatttg cgacaccgag tcgtccgcaa atgctggcac
     2881 agccacctta ttcttttgac ttgtctgtca tgtattgggc accgaccttg gcacttggtg
     2941 gtacgctttt cactcttcct tcagtcatca ctcaggactt taagcaactc tttgcggcta
     3001 tcttttcatt gccaatcgct atctggacat caacaccatc ctttgcagat atggccatgt
     3061 tgtctgaata cttcaacagt gagaaaatgt ctggaatcac gcatttctac tttgatggtg
     3121 aagaattgac ggtcaaaaca gctcaaaaac tgcgcgagcg tttcccaaat gcccgtatca
     3181 tcaatgctta cggcccaaca gaagcgacag tagctctgtc agcagttgcc gtgacagacg
     3241 agatgttagc gactctcaaa cgcctaccaa tcggctatac caaggctgat tctccaacct
     3301 ttatcattga cgaggaagga aataaactgc caaatggtga gcagggagaa atcattgttt
     3361 ctgggccagc tgtttcaaaa ggttatatga aaaatcctga aaaaacagca gaagccttct
     3421 ttgagtttga aggtctgcca gcctatcaca caggcgatgt gggaaccatg acagatgagg
     3481 gcttgcttct ctacggcgga cgcatggact tccagattaa gtttaacggt taccgcattg
     3541 agttagaaga tgtctctcaa aacctcaaca agtctcgctt tatcgaatct gctgtcgcag
     3601 taccgcgcta taacaaggac cacaaggtac aaaatctatt ggcttatgtc atcttaaaag
     3661 acggtgttcg tgagcagttt gagcgagata tcgatattac caaggccatc aaggaagacc
     3721 tgacagacat catgatgtcc tacatgatgc catctaaatt cctttaccga gacagtttgc
     3781 cactaactcc aaatggaaag attgacatca aaggattgat taacgaggtg aataagagat
     3841 gatggagttt tttcaacagc ttcctcattt agagccatat ggcaatcctc agtattttgt
     3901 ttatgtgatt gctgcaacct tgcccatctt tataggcctc tttttcaaga aacgctttgc
     3961 ctggtatgaa gtgttggtaa gtctcttctt tattgtcacc atgttggtgg gtggaaagac
     4021 caatcaacta gctgccttgg gtatttacct ttgctgggaa atattgctcc tgcttttcta
     4081 caagcattat cgaaaaagca aggatggcaa gtgggtcttc tacttagtta gttttctgtc
     4141 cctacttccg attatctttg tcaaggtgca accagctatc aatggaacgc agtctttgct
     4201 tgggttcttg ggaatttctt acctgacctt tcgttcggtt gggattatca tcgagctgag
     4261 agatggagtg attaaggatt ttaccctctg ggaattcctc cgtttccttc tcttcatgcc
     4321 aactttctcg agtggtccaa tcgatcgctt taagcgattt aatgaaaatt atcaggctat
     4381 tcctgagcga gatgagttga tggatatgct ggatgaatct gtccgctata tcatgtgggg
     4441 ctttttgtat aagtttatcc tagctcatgt tttaggagag accttactac ctcctctgaa
     4501 gaatttagcc ttgcagtcag gtggcttctt taatctctat gccttggcag ttatgtatac
     4561 ttttggtctg gagctcttct ttgactttgc aggttattct atgtttgctt tggccatctc
     4621 aaacttgatg ggaatccgta gccctatcaa ctttaacaag ccctttttat caagggattt
     4681 aaaggagttt tggaatcgct ggcatatgag tctgtccttc tggttccgtg actttgtctt
     4741 tatgcgaatg gtgatggtgt taaccagaaa gaaagtcttt aaaaatcgta atgtaacctc
     4801 aagcatggcc tacattgtaa atatgctgat tatgggattt tggcatggtg tgacctggta
     4861 ctatatcgcc tatggactct ttcatggact aggcttggtc atcaatgatg cctgggttcg
     4921 caagaaaaaa acgctcaata aggaacggaa aaaagcaggg aaggctgccc tacctgagaa
     4981 tcgctggatt cagttgcttg gcatggttgt cactttccat gttgtcatgt tgtcattctt
     5041 aatcttttct ggattcttga ataatctatg gtttaaaaaa taaaaggaaa taaaatatgg
     5101 atatcaaatc agaagttatt gaaattattg atgagttgtt tatggaagat gtttctgaca
     5161 tgatggatga agatcttttt gatgcaggtg tcttggatag tatgggaacg gttgagttga
     5221 ttgtggagat tgagaaccgt tttgacattc gtgtccctgt aacagagttt ggtcgtgacg
     5281 actggaatac agctaataaa atcatagctg gtattgtgga gctacaaaat gcttaaacgc
     5341 ttatggatga tcttcggacc ggtcttgatc gctggtttgt tggtttttct gctcattttc
     5401 ttttatccta ctgagatgca tcataatcta ggagctgaaa agcgttcagc agtggctact
     5461 actatcgata gttttaagga gcgaagtcaa aaagtcagag cactatctga tccaaatgtg
     5521 cgttttgttc ccttctttgg ctctagtgaa tggcttcgtt ttgacggtgc tcatcctgcg
     5581 gtattagctg agaaatacaa tcgttcctac cgtccttatc ttttaggaca ggggggagct
     5641 gcatcgctta accaatattt tggaatgcaa cagatgttac cacagctgga gaataaacaa
     5701 gttgtgtatg ttatctcacc tcagtggttc agtaaaaatg gctatgatcc agcagccttc
     5761 cagcagtatt ttaatggaga ccagttgact agttttctga aacatcaatc tggggatcag
     5821 gctagtcaat atgcagcgac tcgcttactg caacagttcc caaacgtagc tatgaaggac
     5881 ctggttcaga agttggcaag taaagaagaa ttgtcgacag cagacaatga aatgattgaa
     5941 ttattggctc gttttaatga acgccaagcc tccttttttg gtcagttttc ggttagaggc
     6001 tatgttaact acgataagca tgtagctaag tatttaaaaa tcttgccaga ccagttttct
     6061 tatcaagcaa tagaagatgt tgtcaaagca gatgctgaaa aaaatacttc caataatgag
     6121 atgggaatgg aaaattattt ctataatgag cagatcaaga aggatttgaa gaaattaaag
     6181 gattctcaga aaagctttac ctatctcaag tcgccagagt ataatgactt gcagttggtt
     6241 ttaacacagt tttctaaatc taaggtaaac ccgattttta tcattccacc tgttaataaa
     6301 aaatggatgg actatgctgg tctacgagag gatatgtacc aacaaacggt gcagaagatt
     6361 cgctaccagt tagaaagtca aggttttacc aatatagcag atttttctaa ggacggtggg
     6421 gagcctttct ttatgaagga caccattcac cttggttggt tgggttggtt ggcttttgac
     6481 aaggcagttg atcctttcct atccaatccc acaccagctc cgacttacca tctgaatgag
     6541 cgctttttca gcaaagattg ggcgacttat gatggagatg tcaaagaatt tcaatagata
     6601 atataatata gaagagttca agaatgaaac tagttttcac gttttttctt gactcttttt
     6661 ttgcttgtga tataattaac tggtaaacaa aatgtcaaag aatagtattt ttctcttaaa
     6721 aaagagaagt ctaaaaggaa aggagtcaga tatgagacag ctagcaaagg atatcaatgc
     6781 ttttttgaat gaggtgattt tgcaggcgga aaatcagcat gaaatcctaa taggtcattg
     6841 cactagcgag gtggccctga ccaatactca ggagcatatc cttatgctct tgtcagagga
     6901 atctttaaca aattcagaat tggcccgtcg tctcaatgtc agtcaggcgg cagttaccaa
     6961 ggccattaag tctttggtca aggaagggat gttggaaaca tctaaagatt ctaaagatgc
     7021 gcgtgtgatt ttttatcagt tgactgactt ggctcgtcca atcgctgagg agcaccacca
     7081 tcaccatgag catacacttt taacctatga acaagtggcg actcagttta ctccaaatga
     7141 acaaaaagtg attcagcggt ttttgactgc tttagtagga gaaatcaaat aatgagatat
     7201 attacggtag aggatttgtc cttctattat gataaggagc ctgttcttga acatatcaat
     7261 tatagtgttg atagtgggga atttgttacc ttgactgggg aaaatggagc ggctaagacg
     7321 acgctcatca aggccagtct tggaattctg caaccacgca ttggaaaggt ggctatttca
     7381 aagacaaata cgcaaggtaa gaaattgaga atagcctatc ttcctcaaca aattgccagt
     7441 tttaatgctg gttttccaag tacggtctat gaatttgtca agtcgggtcg ctatccgaga
     7501 aaaggctggt tccgtcgttt gaatgctcat gatgaggagc atatcaaggc cagtctggac
     7561 tcagttggca tgtgggaaca tcgagacaaa cgcttggggt ctctatctgg gggacaaaag
     7621 cagcgagcgg tgattgcgcg tatgtttgct tctgaccctg atgtgtttat cctagacgag
     7681 ccgacaacgg ggatggatgc aggaagtaaa aatgaatttt acgaactcat gcaccacagc
     7741 gcccatcatc atggcaaggc tgttttgatg attacccatg accctgaaga agttaaggat
     7801 tatgcggatc gcaatattca tctagtccgt aaccaagact cgccatggcg ttgtttcaac
     7861 gttcatgaga atgggcagga ggtgggccat gcttagttta ttatcttatg actttataca
     7921 acgcgccttt ttggcggtta ttgctatgag tcttttctca ccggtattgg gaaccttcct
     7981 catcttgcgt cgtcagagtt tgatgagtga tacccttagc cacgtctcac tttcaggtgt
     8041 agcctttggt ctggttttgg ggatttctcc aactgtttct actattgcca ttgtcttgat
     8101 tgcggcggtc tttctggagt atctccgtac ggtttacaag agctttatgg aaatcgggac
     8161 agctatcctc atgtcaacag gtttggctgt ttctctgatt gtcatgagca agggtaaaag
     8221 ctcgagttca atgagtttgg accaatatct ctttggttcg atcgtgacta tcagtgaaga
     8281 acaggtcatt tccctctttg tcattgcggc ggttgttttg attttgacct ttctctttct
     8341 tcgtcctatg tatatcttaa cttttgacga agatacggcc tttgtggatg gcttgccagt
     8401 tcgtaccatg tccattcttt ttaacatggt gacaggggtg gctattgccc ttatgattcc
     8461 tgcagcagga gctcttctgg tatcgaccat tatggtcttg ccagctagta ttgccctgcg
     8521 tctggggaaa aactttaaat cggttatgct gcttgccagt gcgattggct ttttgggaat
     8581 ggtagcagga ctttacattt cctactatgc agaaacacct gcaagtgcaa gtattaccat
     8641 tatttttgta actgtcttta tactaatcag tttagtaaga cgttttatca aataggagac
     8701 gaacgtgaaa aaaattagct tattactagc cagtctatgt gccttgtttt tagtggcttg
     8761 ttccaatcaa aaacaggcag atggtaaact caatatcgtg acaacctttt accctgtcta
     8821 tgaatttacc aagcaagtcg caggagatac ggctaatgta gaactcctaa tcggtgctgg
     8881 gacagaacct catgaatacg aaccatctgc caaggcagtt gccaaaatcc aagatgcaga
     8941 taccttcgtt tatgaaaatg aaaacatgga aacatgggta cctaaattgc tagatacctt
     9001 ggataagaaa aaagtgaaaa ccatcaaggc gacaggcgat atgttgctct tgccaggtgg
     9061 cgaggaagaa gagggagacc atgaccatgg agaagaaggt catcaccatg agtttgaccc
     9121 ccatgtttgg ttatcaccag ttcgtgccat taaactagta gagcacatcc gcgacagctt
     9181 gtcagcagat tatcctgata aaaaagagac ctttgagaag aatgcagctg cctatatcga
     9241 aaaattgcaa gccttggata aggcttacgc agaaggcttg tctcaagcaa aacaaaagag
     9301 ctttgtgact caacacgcag cctttaacta tcttgccttg gactatggac tcaaacaagt
     9361 cgcaatctca ggactttctc cagatgcaga gccatcagct gctcgcttgg cagaattgac
     9421 agagtatgtc aagaaaaata aaatcgccta tatctatttt gaagaaaatg cctcacaagc
     9481 ccttgctaac acactttcaa aagaagcagg tgtcaaaact gatgtcctca atcctttaga
     9541 aagtctgaca gaagaggaca ccaaggctgg agaaaattac atctctatca tggagaaaaa
     9601 cctcaaggct ttgaaacaaa caacagacca agaaggccca gcgatcgagc ctgaaaaggc
     9661 agaggatacc aagacagtcc aaaatggtta cttcgaggat gcagctgtca aggaccgcac
     9721 cttgagtgac tatgcaggta actggcaatc agtttatcct ttccttgaag acggcacgtt
     9781 tgaccaagtc tttgactaca aggctaagtt gactggtaag atgacccagg ctgagtacaa
     9841 ggcttactat acaaaaggct atcagacaga tgtgactaag attaacatta ctgataatac
     9901 tatggaattt gttcaaggtg gacaaagcaa gaaatatact tacaagtatg tcggtaagaa
     9961 aattttgact tacaagaaag gcaatcgtgg cgtgcgtttc ctctttgaag ccacagatgc
    10021 tgacgctgga caattcaagt atgttcagtt tagtgaccac aatatcgccc cagttaaggc
    10081 agaacatttc catatcttct ttggaggcac aagccaagaa gccctctttg aagaaatgga
    10141 caactggcca acctactacc cagataacct atctggccaa gaaatcgctc aagaaatgtt
    10201 ggcgcattga tgagaaatcg actagttcat aagagctagt cggttttttg atactagatg
    10261 gctaacttca ttatagacct ttcagaacta agggaacctg aaaaaataaa taaaactctt
    10321 gaaatatctg ggcttatatg ctagaatgta gttagtgtat aaggttcatt agaacaccaa
    10381 agcccctaac tagtgccata gttaagggct tttttgacgt atcttagcga tttaacgggt
    10441 gtttataggc tagtcagtcg gtctacttct taccatctag ccatttgcag acgaaataca
    10501 agataattcc agctataaca tctgctataa tagtaagagc aagctctggt ataaggctca
    10561 ttagtttcac ctcctctcac gaaccgtaag gaacgtaatc ggtaaccgat gacaaaaata
    10621 gtatatcaca ttaaatttag atcatcaaca ttgcttaatg cttgaaatat taggacttat
    10681 atgctaaaat gtagttaatt gataaggttc attagaacac caaagcccct aactatggcc
    10741 gtagttaagg gcttttttga cgtatcttag cgatttaacg ggtgtttata ggctagtcag
    10801 tcggtctact ccttactatc tagccatctg cagacgaaat acaagataat tccagctata
    10861 acatccgcta taatagtaag agcgagctct atgataaggc tcattagttt cacctcctct
    10921 cacgaaccca taggaacgta atcggtaacc gatgacaaaa atagtatacc acaatacatt
    10981 tagatcatca aggtcactta attcttgaaa tatagggtct aggcgacaaa gtctttaaaa
    11041 tcagaaaaac gcataatatc aggtgtgcaa aaacttgata ctatgcgttt tattgtggga
    11101 atatttgccc catttctcct gaaattgagt ttttgtccag cctctgtttt tagggttgct
    11161 aagaaaataa tgtcatgtgg tgaatatttg taaatcagtc agcagacaga acgatactct
    11221 tcgaaaatct cttcacatca tgtcagcttc gtcttgccgt gtccatgggg gccttgactt
    11281 catcagtttt caaatctgta ctgtgagcaa gctgagacta gcttcctatt tgatttttat
    11341 tgaatatcag aaacccattc tccatcaaat aattcgactg cgtctaataa tttttggtct
    11401 ggcaaggtgt cagaaataaa ggttgtgtat ttggataggg gattaatttt aaaaaatcca
    11461 gtcttgtaaa atttagaact atcaatcagt aagatggttt catgggcttt gtcaataata
    11521 ttcttttttg aaatagcttg gctgagagaa gcttcataaa catattggtc atcaatacct
    11581 cttgctgaac aaaatgctaa atcgatatta aaatgatcta ataaagaatt ttccttatca
    11641 tagttgacca cggaacagga ttgatgtttg acctcgccag atgtgataaa gattttggag
    11701 ctatctttaa cagtttcaga tagggtttgt gcagtatgta aaccatttgt aaaaataatc
    11761 aaattatcaa gttcagaaag atagggacaa agttcgtaga cagtagtact agaatctaga
    11821 tagatacaca taccagaccg aataaagtct ttagcgagac tagcgattag tcttttttgc
    11881 ctagtacttt ctccttcccg tatttgatga gaaagttcaa ttgtgttcat agaggacagg
    11941 gtcacgtatc cgtgctttct tttgataaga ccttgatttt ctaagaaaat taaatcacga
    12001 cgtaaggtac ttgtgctgga gaaagtgatt tctgccagct cttttacggc aattcttttt
    12061 ttctttttga tgatttcaat caattcaagt acacgttcat cttttatcat aagctcctcc
    12121 taatttatca tttcaactat attatagcac aaattggagg aatgtgaatt atttttatga
    12181 atattgggtt aacatttgaa cattattcaa gtaagcgttc acatattgaa aaaataaaac
    12241 gtggggatta taataaagtt aatcaaggac gaagagagaa gaaaaatgga agcggtttta
    12301 gcaatagatt taggtgcgac ttctggaaga gcaatcgttg gttacctttc tgaaaataaa
    12361 ctagtaatgg aagaaataaa tcgcttttct aatctaccta ttagagtaaa agggcattta
    12421 tcttgggatg ttgactttct actagctaaa attcttgaaa gtatccgctt ggctaatact
    12481 agttacaaga ttttatctat cggtattgac acatggggag ttgattttgg actgattgat
    12541 aatgaaggta agctgttatc acaacctgtt cattatcgtg atgaaagaac aaagggagtg
    12601 ttaaaggaaa tatctgaaat gactgaatta gaaaaactgt attcagagac aggaaatcag
    12661 attatggaga taaatacctt gtttcaactc tttaaggcac gtcaagaatc tcctgactct
    12721 ttctataaga ccaataagat tcttttaatg ccagatttgt ttaattatct cttgacaggt
    12781 aagtttgcta cagaaaaaag cattgcttca acaactcaat tatttgatcc taggagtcaa
    12841 aattggaatc agaatatctt aaaactattt gaattggatt caactttact tcctgaaatt
    12901 gtttcagagg gaaatgttct tggaaggata aaagaggagt atggtttagg cgatattcct
    12961 gttgtgaatg tttgtagtca tgatacagca agcgcgattg tctcagtacc taagacagaa
    13021 ggtagtttat ttatttcatc aggtacttgg tctttggttg gagtggaact tacttcaccg
    13081 attcttacta ccgaatcctt cagttatgga tttacaaatg aagtcggtaa agatggagtg
    13141 attacatttc tgaagaattg tacagggttg tggatcatag aggaactaag acgttcattt
    13201 gaacgaagag ggaaagccta ttcttttgat gatattagga caatggtgga gaaagaaaaa
    13261 gaaaatcttc ctctgattga tactgaatca actgaatttg caacagaatc tgatatgcac
    13321 aagactttga cagaatatct agtttatcat catgaaacta gagagtggac agatggacaa
    13381 ctatttaaga ttgtttatga aagcctagct gaaacgtata ggaaagcgat agagttacta
    13441 gaagaactaa ctcataaggt ttataagagg atatatgtga ttggaggagg tgctagagcc
    13501 agttacttta accaaatgat tgctgataga actggtaaag aggttcttac aggtttgact
    13561 gagggtacag ctgtggggaa tattgttgtg cagctcatag ctatgggaca attaaaaggg
    13621 atggaagagg ctcaccatgt tattgaggag tttctacaat tagagagtta ttactcccaa
    13681 aagaattaaa aagattgaga gtttgtaaat ttgcctccct cccccttctt agcttttgtg
    13741 caggaagggg ggataattgg tgaattgaaa aatatttagt gttttgatat gaggaggaca
    13801 aggatgtcag atgtaaaaca agaattaatt aaatatggta agaagctagt agaaacagat
    13861 ttgacgaaag gaacaggtgg gaatctcagc gttttcgatc gtgaaaaaca attgatggca
    13921 attaccccgt cgggtattga tttctttgaa atcaaagaat ccgatattgt agtgatggat
    13981 attaatggaa atgttgtaga gggagaacgc ttgccatcta gcgaatggta tatgcatttg
    14041 attcaatatc aaactcgtga tgatatcgat gcaattatcc atgctcatac aacttatgca
    14101 acagtattag cttgtctcag agaaccactt ccagcgagtc attatatgat tgcagtggca
    14161 gggaaagatg ttcgggtagc tgagtatgca acatatggca cgaaagaatt ggctgtgaat
    14221 gcagctaaag caatggaagg tcgtagagca gttttactag cgaatcatgg aattttagca
    14281 ggtgcacaaa atttattgaa tgcatttaat attgttgaag aagttgaata ttgtgcaaaa
    14341 atttattgtt tagctaagaa ttttggagag ccagtagttc ttcctgatga ggagatggaa
    14401 ttgatggcag aaaaatttaa aacatacggt cagagaaaat agggaggata ttaatgttaa
    14461 aacatatacc gaaaaatatt tctccagatt tattgaagac tttaatggaa atgggacatg
    14521 gagatgaaat agtattagct gacgcgaatt atccttctgc ctcatgtgca aataagctaa
    14581 ttcgttgtga tggtgtaaat attccagaat tattagattc cattctgtat ttaatgccat
    14641 tagatagtta cgtcgatagt tcaattcagt ttatgaacgt tgtttcgggt gatgatattc
    14701 ctaagatatg gggtacctat agacagatga ttgaaggtca tggtacagat cttaaaacga
    14761 ttacttatct tagaagagaa gacttttatg aacgtagtaa gaaagcttat gctattgttg
    14821 ctacaggaga aacttcactt tatgctaata ttatccttaa gaaaggagta gttgttgaaa
    14881 gagaaaatgt ttaatagagg aattttagtt gccagtcatg gtaattttgc tagcggagct
    14941 ctcatgaccg cagaaatgtt tgttggtgag acaacaaatg atagagttag gacattaggt
    15001 ttgatgcctg gagagaatat tgtagagttt gaacattatt ttaaaaatca agtaaatgaa
    15061 ctgttagact caaatcaaga ggttatcgtt ttgactgact tgattggagg aagtcctaat
    15121 aatgtggctt tgtcacggtt tttaaatttg gattcagttg atattgtaac agggtttaat
    15181 atccctctcc tagtggaatt aatatcaagt tatgattcaa aaatcaattt agaagaaatt
    15241 gttcaaaatg ctcaaaatag tttgtttaat gttaaacaac aacttaacgt agaggaggaa
    15301 gaggatttat gcctatagag tttgttcgta ttgatgaccg tctggtacat ggtcaagttg
    15361 tcactacgtg gctaaaaaag tatgatattg agcaagttat cattgttaat gatcgcatct
    15421 cagaagataa aacacgacaa tctattttaa agatttctgc accggtaggt ttaaaaattg
    15481 ttttctttag tgtaaaacgg tttgtggaag ttttaaactc tgtgccaata aaaaagagaa
    15541 caatgctgat atatacaaat ccaaaagatg tgtatgattc tattgaagga aatttaaaat
    15601 tggagtacct caatgtagga cagatgagta aaacggagga aaatgaaaag gtaacgggag
    15661 gtgtagctct aggtgaagaa gacaaatatt attttaagaa aatagttgat aagggaacga
    15721 gagttgaaat tcaaatggtt cctaatgata aagttacaat gttggaaaaa tttttataaa
    15781 aataatttaa ggaggtacag tatatgctat tcacacaagc attactggtg acattagttg
    15841 ggattattgc cactattgac tataatggac cgttatttat gattcaccgt ccgttagtta
    15901 caagtgcaat ggttggctta gtcttaggag atttcaccca aggtgttctt attggttcag
    15961 ctcttgaatt aacttggctc ggtgtaacag gtattggagg ttacactcca ccagatacta
    16021 tttcaggtgc gattattggt actgcatttg gtattttgtc tggtcaagga gaaactgctg
    16081 gtatcgctat agcagttcca attgcagttg ctactcaaca gttggatgtt cttgcaaaaa
    16141 ctttagatgt ttattttgtg aaaaaagctg ataatgatgc taaaaacgga gattattcaa
    16201 agatcggttt ttatcattat tcaagtttgg ttttaatcac tttatttaaa attgtaccaa
    16261 ttttcctagc tattatgctt ggaggggaat atgtggcaga cttgtttgct aaggttccac
    16321 caatcgttat gcagggactt aactctgcag gtgctttact accttcaatt ggttttggta
    16381 tgcttttaaa tatgatgctc aagaaaaata tgtgggtatt cttgttgatt ggattcattt
    16441 gttctgtgta tggaggaatg tcaaccattg ggatctcact agttggtatt gcggtagcat
    16501 acttctacga tatgattgga agtaaaccac aagaaacaac ttcaagtagt gatgttgagg
    16561 aggatcttga tctatgatga ataataaagt aactaaagtt gaacttaaaa aagttttcaa
    16621 acgaagtttt atgtatggtt cttcatggaa ctatgagaga atgcagaacc taggttttct
    16681 atatacaatt cttccagtat tgaaaaaact atacccagac aaagattcag cttctcctgc
    16741 aatgaaacgt caccttgagt ttttcaatac tcatcaaaca gcggcaccat ttattcttgg
    16801 agttacttcc gctatggaag aacaagaagg aaatgaaggt gcagcttcaa ttactggtat
    16861 taaagttggc ttgatggggc cactggctgg tctaggagat agtttgttct ggctgacact
    16921 agttcctatc tgttttagta ttggtgcgtc ttattctaaa gacggcggtg ctttaggtat
    16981 ctttatcgcc ttaatattgt ttaatattat taatattcct gttaaatatt tcggtttgaa
    17041 atatgggtat actaagggtt ctagtcttat ccaagaaaat aatacaaaag gaacattgaa
    17101 tcgcgttacg agtatggcga cagcattagg gctagtacta gtgggtggtt tgattccatc
    17161 aatggttggt attaattttg gattagaatt taagcagggg gaacttgtta tttctgttca
    17221 agaaatgatt acaaaattaa ttccaggatt tatccctatg gctttgactt tattaatgtg
    17281 taaattaatt agaaaaggaa agaatccggt tgtactaatc tttagtgtta tggctattgg
    17341 agttatccta gttgttttag gaattttgaa gtagtagaaa gtgtggaggt ggtatttggg
    17401 atatcacctc cattttggaa gagaggtaaa gagtgaaatt atggtataag aaagctgcct
    17461 caaattggaa tgaagccttg ccgattggga acggtcattt aggtggtatg atttatggtt
    17521 cagctacaaa agaatgtatt caactaaacg atgagactat ttggtataga ggaaagtcag
    17581 atagaaataa tccagactca ctattgcatc ttaaaaaaat tcgggaatat cttttagatg
    17641 gagaaattca gaaagccgaa gaattgataa agttaacaat gtttgctacc ccaagagatc
    17701 aaagccacta tgaattactt ggggaacttt acattgagca tatagatatt cagtcttgtg
    17761 ctctttcatt gtatgaaaga gagctagatt tagatacagc tatttctaat gttgtgtttg
    17821 agcctaatag ttgtaattta caaataaaaa gagaatattt tacgagtttt aataagaata
    17881 ttttatgttg ccgtatagtg tcatcagttc aaaacacatt aaatttaaac attaatttgg
    17941 gtagaaataa acggtttaat gacgaagtat ctaaactgga ttcaagtaca attttaatgt
    18001 cggcctctgc tggaggtaga aaaggtgttc agtttaaagt agtatgtcat tctaaagtta
    18061 cggatggtga agtaagtgta ttgggagaga caatagttat tcggaatgct acagaggtat
    18121 ttctttatct caaatcaatg acggattatt ggggaaatat agatatttct tctcttcagg
    18181 gagaatttag tagtattgat tactttacag aaaaagatga acatgtaaaa aaatatcagg
    18241 agcaatttaa tagagttgat tttaaactag actatagtaa agattgtctt agcattccaa
    18301 cgaatctact tcttgaaaac actaaaaagt atagtaacta cttgactaac ttgttatttc
    18361 attatggaag atatctgtta atatcgtcta gtcaaccgaa tggtttacct gtcaatcttc
    18421 aaggaatatg gtgtgatgaa ttaaatccaa tttggggttc taaatatacg attaatatta
    18481 atactcaaat gaattattgg atggtaggtc catgtgattt accagaagta gaatatccat
    18541 tatttgatat gctcgaaaga atgagagaac cgggaagact aaccgctaag aaaatgtatg
    18601 gagctagagg ttttacagca catcataata cggatggttt tggcgatacg gctccccaat
    18661 ctcatgccat gggggctgca atttgggtat taactattcc atggttatgt actcatattt
    18721 gggaacacta tttatatttc caagatgagc gtattcttac ggaacatttt gaaatgataa
    18781 aagaagcatt tcttttcttt gaagattatt tatttgaggt ggatggctac ttgatgacag
    18841 gtccaagtgt ctcaccggaa aataaatatc gcttaaaaaa tggtattgaa ggaaatgctt
    18901 gtctatcatc tacaattgat aatcaaattc taagatattt ttgtgattca tgcattggca
    18961 ttgcaaaaca attaggagac aattcggatt ttattagtcg tgtgaaggag ttaaaaaaga
    19021 aactacctaa aacaaaaata ggtagtaatg ggcaaatcca agaatggtta gaagattatg
    19081 aagaagtaga gcctgggcat agacacattt cacctctatt tgggctttat ccttataatg
    19141 agattgatat tcataaaact ccggaattag cagaagcagc taaaatcact atcaatagga
    19201 gattatcaaa cgctaacttt ttatcttcac aggagaggga gcaagcgatt aataattggt
    19261 tagtaagtgg tttgcatgct agtacacaaa caggttggag tgctgcatgg ctgattcatt
    19321 tttttgcgag actatatcaa ggtgaacctg cttataacca gattaatggt ttgttaaata
    19381 atgcgactct tggcaattta tttcttgacc atccaccatt tcaaattgat ggtaatttag
    19441 gtttggtgag tggaatttgt gaattattag tacagagcca tcataattgg ttatcactaa
    19501 ttccagcttt accttctgct tggtcagaag gagaagtgaa aggtttcaga gtaagaggag
    19561 gatataaggt atcgtttgct tggaaaaatg gggatataac attcctaaaa ttggaaggag
    19621 gaaacaaaga tcaaaaagta agagtaagaa tatatggcaa aaatactgat gtacaaaata
    19681 ttgaattggt atttaattca gaaaaaatta ttgagttaaa tttttaggta taagtcatga
    19741 ataaagaaaa aataaaaaga aaattaatca caatactgtt tgtatgtatt gggatgttat
    19801 gttttggatt gttagcagga gttaaggctg ataatcgtgt tcaaatgaga acgacgatta
    19861 ataatgaatc gccattgttg ctttctccgt tgtatggcaa tgataatggt aacggattat
    19921 ggtgggggaa cacattgaag ggagcatggg aagctattcc tgaagatgta aaaccatatg
    19981 cagcgattga acttcatcct gcaaaagtct gtaaaccaac aagttgtatt ccacgagata
    20041 cgaaagaatt gagagaatgg tatgtcaaga tgttggagga agctcaaagt ctaaacattc
    20101 cagttttctt ggttattatg tcggctggag agcgtaatac agttcctcca gagtggttag
    20161 atgaacaatt ccaaaagtat agtgtgttaa aaggtgtttt aaatattgag aattattgga
    20221 tttacaataa ccagttagct ccgcatagtg ctaaatattt ggaagtttgt gccaaatatg
    20281 gagcgcattt tatctggcat gatcatgaaa aatggttctg ggaaactatt atgaatgatc
    20341 cgacattctt tgaagcgagt caaaaatatc ataaaaattt ggtgttggca actaaaaata
    20401 cgccaataag agatgatgcg ggtacagatt ctatcgttag tggattttgg ttgagtggct
    20461 tatgtgataa ctggggctca tcaacagata catggaaatg gtgggaaaaa cattatacaa
    20521 acacatttga aactggaaga gctagggata tgagatccta tgcatcggaa ccagaatcaa
    20581 tgattgctat ggaaatgatg aatgtatata ctgggggagg cacagtttat aattttgaat
    20641 gtgccgcgta tacatttatg acaaatgatg taccaactcc agcatttact aaaggtatta
    20701 ttcctttctt tagacatgct atacaaaatc cagctccaag taaggaagaa gttgtaaata
    20761 gaacaaaagc tgtattttgg aatggagaag gtaggattag ttcattaaac ggattttatc
    20821 aaggacttta ttcgaatgat gaaacaatgc ctttatataa taatgggaga tatcatattc
    20881 ttcctgtaat acatgagaaa attgataagg aaaagatttc atctatattc cctaatgcaa
    20941 aaattttgac taaaaatagt gaggaattgt ctagtaaagt caactattta aactcgcttt
    21001 atccaaaact ttatgaagga gatgggtatg ctcagcgtgt aggtaattcc tggtatattt
    21061 ataatagtaa tgctaatatc aataaaaatc agcaagtaat gttgcctatg tatactaata
    21121 atacaaagtc gttatcgtta gatttgacgc cacatactta cgctgttgtt aaagaaaatc
    21181 caaataattt acatatttta ttgaacaatt acaggacaga taagacagct atgtgggcat
    21241 tatcaggaaa ttttgatgca tcaaaaagtt ggaagaaaga agaattagag ttagcgaact
    21301 ggataagcaa aaattattcc atcaatcctg tagataatga ctttaggaca acaacactta
    21361 cattaaaagg gcatactggt cataaacctc agataaatat aagtggcgat aaaaatcatt
    21421 atacttatac agaaaattgg gatgagaata cccatgttta taccattacg gttaatcata
    21481 atggaatggt agagatgtct ataaatactg aagggacagg cccagtccct ttcccaacac
    21541 cagataaatt taatgatggt aatttgaata tagcatatgc aaaaccaaca acacaaagtt
    21601 ctgtagatta caatggagac cctaatagag ctgtggatgg taacagaaat ggtaatttta
    21661 actctggttc ggtaacacac actagggcag ataatccttc ttggtgggaa gtcgatttga
    21721 aaaaaatgga taaagttggg cttgttaaaa tttataatcg cacagatgct gagactcaac
    21781 gtctatctaa ttttgatgtg attctatatg acaataatag aaacgaagtt gctaagaaac
    21841 atgttaataa tttgtcgggt gaatctgtta gtctagattt caaagaaaaa ggagcaaggt
    21901 atattaaagt taaattacta acgagtggag tgcctttgag tttagcagaa gtagaggttt
    21961 ttagagaatc agatggtaag caatctgaag aggatataga taaaataaca gaagataaag
    22021 tagtctctac aaataaggta gctactcaaa gttcaaccaa ttatgagggt gtagctgctt
    22081 tagcagttga tggtaataaa gatggagatt acggacatca ttcggtgact catactaagg
    22141 cagattctaa cgcttggtgg caggtcgatc tgggagaaga gtttacggtt tctaaagttg
    22201 atatttataa tagaacagat gccgaacctc agcgtttatc taattttgat gttatttttc
    22261 tatcttcatc aggagaagaa gtttttagaa gacattttga taaagtagtt gatggtttgt
    22321 tatctttaaa agtaccttct gtaggggcta agctagtcaa aatagaatta aaatcagcag
    22381 ctattccgtt aagtttagcg gaagttgaag tctatggttc aaagagaact ccgaagaaac
    22441 tttctaatat tgcattaacc aaagaaactc gacagagttc aacggattac aatggttttt
    22501 ctcgtctagc agttgatgga aataaaaacg gagattatgg tcatcattca gtgactcata
    22561 ccaaaggaga ttctccttca tggtgggaga tagatttagc acaaaccgaa gaattagaaa
    22621 agttaattat ttataataga acagatgctg aaattcagag attatcaaat tttgatatta
    22681 ttatatatga ttcaaataat catgaagttt ttaaacaaca tattgacagt ttagaaagca
    22741 ataatctatc catagactta aaagggctga agggaaaaaa ggttagaatt tctttgagaa
    22801 acgcaggaat tcctttaagt ttggcagagg tagaggttta tacttataag taattttaaa
    22861 aattatcatc ggtagtatga aagaaacaga aaaataagag gaaaatagta tgattcaaca
    22921 tccacgtatt gggattcgtc cgactattga tggtcgtcgt caaggtgtac gcgaatcact
    22981 tgaagtacaa acaatgaaca tggctaaaag tgtggcagat ttgatttcaa gcacattgaa
    23041 atatccagat ggggaacctg tggaatgtgt gatttctcca tctaccattg gtcgtgttcc
    23101 agaggctgca gcttcccatg agttgtttaa aaaatcaaat gtttgcgcaa caattacagt
    23161 tacaccatgc tggtgttatg gtagtgaaac tatggatatg tctccagata ttcctcatgc
    23221 tatttgggga tttaatggga cagaacgccc aggagctgtc tatcttgcag ctgtactagc
    23281 ttcacatgct caaaaaggga ttccagcctt tgggatttat ggaagagatg ttcaggaagc
    23341 tagtgacaca gctattccag aagatgtcaa agaaaaactt ttacgttatg cgcgtgcagc
    23401 tcttgcaact ggcttgatga gagacactgc ttacctatca atgggtagtg tttcgatggg
    23461 gattggtggt tctattgtaa atccagattt cttccaagaa tacttaggaa tgcgaaatga
    23521 atcggtagat atgacggagt tcacgcgccg tatggaccgt ggtatttatg acccagaaga
    23581 gttcgaacgt gctatggttt gggtaaaaga acatatcaaa gaaggggttg accgaaatcg
    23641 tgaggatttg attctttcta aagaagagaa agaaaaacaa tgggaatttg ttattaagat
    23701 gttcatgatt ggacgtgact taatggttgg taacccaaga cttgctgaac ttggttttga
    23761 agaagaagca gttggtcacc atgctttagt agctggtttc caaggtcaac gccagtggac
    23821 agatcatttc ccaaatgggg actttatgga aactttcctc aatactcagt ttgactggaa
    23881 tggtattcga aaaccatttg tatttgcgac agagaatgat tcactaaatg gtgtgtctat
    23941 gctctttaat tatctattaa caaatactcc acaaatattt gctgatgtgc gtacttattg
    24001 gagtccagag gctgttaaac gtgtaacggg acatacttta gagggtcgtg ctgcagctgg
    24061 cttcttacat ctaatcaact ctggttcttg tacattggat ggtacaggtc aagctactcg
    24121 agatggcaaa cctgttatga aaccattctg ggagttggaa gaaagtgaag tgcaggctat
    24181 gcttgaaaat acagacttcc caccagcaaa ccgcgaatac ttccgtggag gaggattctc
    24241 aactcgtttc ttgacgaagg gggatatgcc agtaacaatg gtacgtctca atcttctaaa
    24301 aggggttggt ccagtgttac aaattgcaga aggttacaca cttgaacttc ctgaagatgt
    24361 tcaccatact ttagataatc gtacagatcc aggatggcca actacttggt ttgctccacg
    24421 tttgacagga aaaggtgctt tcaagtctgt ctatgacgtc atgaataatt ggggagctaa
    24481 tcacggagcc ataacatatg gacacattgg agcagacttg attaccttgg cttctatgtt
    24541 gagaattcct gtcaatatgc ataatgtacc tgaggaagat atctttagac ctaaaaattg
    24601 gtccttattt ggaacagaag atctagaatc agcagactat cgtgcatgtc agttgttggg
    24661 accactacat aaataaaact tgtttatata ggaggtgaac ttacgtccct cctatccttt
    24721 taaaaagatt tgttaaacaa ttcacaaata attaaaaacg aatacaaaaa gtaatataat
    24781 gatgttaaat agatagcgca aaggcgcagg aggaaaatta tatggctaca ttttatgttc
    24841 cggcagtcaa ccttattgga aaaggtgttg taaatgaagt gggtccttat atcaaggaac
    24901 ttggctataa aaaggcactt ttggtgacag ataagtacat cgaaggcagt gacattttac
    24961 ctaaggtttt aaaaccactg gatacagaag gaatcgaata tgtcatcttt agcgatgtag
    25021 agccaaaccc tacttgtaag aatgtcacag atggggtagc tgctttgcaa gaacatggat
    25081 gtgattttat catcagtctt ggcgggggtt ctccacagga tgcagctagt tgtatttcta
    25141 tcatggttac aaatggtgga aaaccacagg attatgaagg gcttcataag tctgctaaaa
    25201 aaggccttcc tgttgtagct atcaatacta cggcaggtac atcagcagaa attaccatta
    25261 actatgtgat tactgatgaa gaacgcaagg ttaagatggt aatggttgac aagaatagcc
    25321 ttgccttgat ttctgtcaat gaccctgaac tcatgctttc caaacctcaa gccttgactg
    25381 ctgctactgg tatggatgct ctgactcacg ctgttgaagc tttggtaaca cctggtgctt
    25441 atgatgtaac caagaaactg tctattggtg ctattgagct tatcaaggaa tatcttcctc
    25501 gtgctgtagc aaacggacat gatattgaag cgcgtgaagg tatggtcaat gccatcttcc
    25561 ttggtggtat gagctttaat aatgctggtc ttggctatgt tcactcaatg gctcaccaac
    25621 tcggtgcagt atataatttg ccacatggtg tttgctgtgc catgttgcta ccagttatag
    25681 aacgtgaaaa tgctaaacgt gtaccagaag ctttccgcaa tgttgccaaa gccttgggac
    25741 ttcatgtaga aggtaaatca gatcaagaat gtgccgatta tgcgattgct gagattgaga
    25801 agctttctga gacagtaggt attcctaaga aactgactga acttggtatt gaagaaaaag
    25861 atttcgactt tgaatacctt tctaagaatg ccttgattga tgcctgcgca ccaggaaatc
    25921 catttatgcc aaccttagaa gaaacgattg ccttttataa agagttattt tagcaaggat
    25981 tggacgacaa taggacaatt cacctctaat tattgacatt tcagaaaaaa tcgcagtaaa
    26041 aacggcacag ccttctttaa atgtgctata atactagaaa aatacttgtg gaggttccat
    26101 tatggcaata tttttcatga tttttctgat tgtttgtgtg ctcctattgg tgatagtcac
    26161 actgagtaca gtttatgtgg ttcgtcagca gtcggtggcg attattgaac gctttgggaa
    26221 ataccaaaag gttgctaata gcggtattca tattcgcttg ccttttggga ttgactcgat
    26281 tgcagcacgg attcagttgc gcttgttgca aagtgatatt gtggttgaga ctaagaccaa
    26341 ggacaatgtg ttcgttatga tgaatgtagc gactcagtac cgtgtcaacg agcagagcgt
    26401 gacagatgct tactataaac tcatacgtcc agaatctcag attaaatctt atatcgaaga
    26461 tgctcttcgc tcttctgttc caaaattaac cttggatgaa ttgtttgaga aaaaagatga
    26521 gattgccctt gaggttcaac accaagtagc agaagaaatg accacttacg gctacattat
    26581 cgtgaaaacc ttgattacca aggtcgaacc ggatgcagaa gttaagcaat ccatgaatga
    26641 aatcaatgcg gcgcaacgta agcgggtcgc agcacaagaa ttggcggaag ctgacaagat
    26701 taaaattgtc actgcagctg aagcagaagc agaaaaagac cgccttcatg gtgtggggat
    26761 tgcccaacaa cgtaaggcga ttgtggatgg attggcagag tctatcaccg aactcaagga
    26821 agccaatgtt ggcatgacag aagaacaaat catgtctatc ctcttgacca accagtattt
    26881 ggataccttg aatacctttg cctctaaagg aaatcaaacc atctttttac caaatactcc
    26941 aaatggtgtg gatgatatcc gtacacaaat cttgtcagcc cttcgcgctg agaagaaata
    27001 atagactaat actcttcgaa aatctcttca aaccacgtca gcgtcgtctt gccgtataga
    27061 tgttactgac ttcgtcagtt ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt
    27121 cagtcttatc tactacctca aaacggtgtt ttgagcaacc tgtagctagt ttcctagttt
    27181 gctctttgat tttcattgag tataaagagc ttggcaacag gctctttttg tattgcgttt
    27241 attaagaaaa acaaaaaaca tcgatataca tttaactaaa aattattgta tgttatctcc
    27301 cttaattagg aatgataagg gaataactag aaagatttgt gaatacaaac tatttctgat
    27361 atacaaaata tacagtaata atgaatgatg ggagatggga tgaaagaatt tcaatttgag
    27421 agaaagcagc gtttttcttt gaggaaatat gcaataggag cttgttcggt cttgctagga
    27481 acgagtttat ttttttgctg gtatgggtgc tcagcctgta caggatacag aaacgagttc
    27541 agcactaatt tcaagtcatt atttggatga gcaggattta tctgaaaagc tgaaatctga
    27601 gttgcaatgg tttgaattag aaaacaagct tttgaactta tgggagcatt aggttactat
    27661 gaaggatttg ttccttatgt ttcaaatcaa tacaaaaacc aagctgaaga agaaggcaaa
    27721 ccgctatctg ataaatatat tttcgaaaaa atcttaggaa aaacatatgc agccttcaaa
    27781 aaagatcaaa ttaatgagcg tgtcgagaaa ttaggtaagt taaaacctat tacaataaat
    27841 tacaacggaa aatcagaagt aattgatagt aaagaaaaat tacaagagct tatgaataaa
    27901 gccgttaaag acgaagtggc tcaaatatag taaaatgaaa taagaacagg acaaatcaat
    27961 caggacagtc aaattgattt ctaaaaatgt tttagaagta gaggtgtact attctagttt
    28021 caacatacta taaaatcagg caatacaacg gctaaaaagt ttgagtttat cgaaatacca
    28081 gtgcaaaaac tgaaaaaagc gatttataaa gctcatctca aagattcaga tgactttaga
    28141 ccagaaac
//
LOCUS       DAVLTM010000014        28120 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLTM010000014 DAVLTM010000000
VERSION     DAVLTM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.014"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(17..694)
                     /locus_tag="VYN16_000515"
     CDS             complement(17..694)
                     /locus_tag="VYN16_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403091.1"
                     /translation="MMSNKNKGILIFVILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(691..1254)
                     /locus_tag="VYN16_000516"
     CDS             complement(691..1254)
                     /locus_tag="VYN16_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1403092.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQSPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKCLK"
     gene            complement(1264..1857)
                     /gene="ruvA"
                     /locus_tag="VYN16_000517"
     CDS             complement(1264..1857)
                     /gene="ruvA"
                     /locus_tag="VYN16_000517"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1403093.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     regulatory      2014..2128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2262..3362
                     /gene="ribD"
                     /locus_tag="VYN16_000518"
     CDS             2262..3362
                     /gene="ribD"
                     /locus_tag="VYN16_000518"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1403094.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     gene            3347..3982
                     /gene="ribE"
                     /locus_tag="VYN16_000519"
     CDS             3347..3982
                     /gene="ribE"
                     /locus_tag="VYN16_000519"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1403095.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            4002..5207
                     /locus_tag="VYN16_000520"
     CDS             4002..5207
                     /locus_tag="VYN16_000520"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1403096.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            5208..5675
                     /locus_tag="VYN16_000521"
     CDS             5208..5675
                     /locus_tag="VYN16_000521"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1403097.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(6000..7949)
                     /gene="mutL"
                     /locus_tag="VYN16_000522"
     CDS             complement(6000..7949)
                     /gene="mutL"
                     /locus_tag="VYN16_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1403098.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            8305..8745
                     /locus_tag="VYN16_000523"
     CDS             8305..8745
                     /locus_tag="VYN16_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1403099.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            8742..9200
                     /locus_tag="VYN16_000524"
     CDS             8742..9200
                     /locus_tag="VYN16_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1403100.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(9418..10371)
                     /locus_tag="VYN16_000525"
     CDS             complement(9418..10371)
                     /locus_tag="VYN16_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1403101.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNKNKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            10755..12017
                     /locus_tag="VYN16_000526"
     CDS             10755..12017
                     /locus_tag="VYN16_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1403102.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(12310..12780)
                     /gene="nrdI"
                     /locus_tag="VYN16_000527"
     CDS             complement(12310..12780)
                     /gene="nrdI"
                     /locus_tag="VYN16_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1403103.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12858..13391)
                     /locus_tag="VYN16_000528"
     CDS             complement(12858..13391)
                     /locus_tag="VYN16_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403104.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            complement(13443..14729)
                     /locus_tag="VYN16_000529"
     CDS             complement(13443..14729)
                     /locus_tag="VYN16_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1403105.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFDQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(14741..16387)
                     /locus_tag="VYN16_000530"
     CDS             complement(14741..16387)
                     /locus_tag="VYN16_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1403106.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(16389..17003)
                     /locus_tag="VYN16_000531"
     CDS             complement(16389..17003)
                     /locus_tag="VYN16_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1403107.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            17138..17707
                     /locus_tag="VYN16_000532"
     CDS             17138..17707
                     /locus_tag="VYN16_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1403108.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPHFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(17736..18428)
                     /locus_tag="VYN16_000533"
     CDS             complement(17736..18428)
                     /locus_tag="VYN16_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403109.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(18430..19491)
                     /locus_tag="VYN16_000534"
     CDS             complement(18430..19491)
                     /locus_tag="VYN16_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1403110.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            complement(19484..20857)
                     /locus_tag="VYN16_000535"
     CDS             complement(19484..20857)
                     /locus_tag="VYN16_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1403111.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(20957..21811)
                     /locus_tag="VYN16_000536"
     CDS             complement(20957..21811)
                     /locus_tag="VYN16_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1403112.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSTNKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            complement(21965..22795)
                     /locus_tag="VYN16_000537"
     CDS             complement(21965..22795)
                     /locus_tag="VYN16_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1403113.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            complement(22898..23221)
                     /locus_tag="VYN16_000538"
     CDS             complement(22898..23221)
                     /locus_tag="VYN16_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1403114.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            complement(23211..23867)
                     /locus_tag="VYN16_000539"
     CDS             complement(23211..23867)
                     /locus_tag="VYN16_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1403115.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTDLGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            23938..24015
                     /locus_tag="VYN16_000540"
                     /pseudo
     CDS             23938..24015
                     /locus_tag="VYN16_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(24321..25547)
                     /locus_tag="VYN16_000541"
     CDS             complement(24321..25547)
                     /locus_tag="VYN16_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1403116.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLKHTISIKTALYASLVTQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(25619..26284)
                     /locus_tag="VYN16_000542"
     CDS             complement(25619..26284)
                     /locus_tag="VYN16_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403117.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(26352..26552)
                     /locus_tag="VYN16_000543"
     CDS             complement(26352..26552)
                     /locus_tag="VYN16_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1403118.1"
                     /translation="MLNLQFAETMELTEAELETVYGGELGYNAVIPGGSWGGLGTSWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            complement(26838..27701)
                     /locus_tag="VYN16_000544"
     CDS             complement(26838..27701)
                     /locus_tag="VYN16_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1403119.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKRYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLTSLFE
                     LRFQQYKELID"
BASE COUNT         8668 a         5984 c         5307 g         8161 t
ORIGIN      
        1 ttgtcctgtt ctcatttcat tttactatac tgatagctaa aatgattaag gagaggctgt
       61 tgcttaacat gtgaacaagt aggggatagt agatattctc tttttctttc acataaataa
      121 tagaaaaggc aaggcctcca cctaagtaac caactgcacc aatccactct gatagagcca
      181 aactgtgcat atgagtcaaa gcaaaaacaa gtcctaccag aataatgctt agtaaacctg
      241 acaaccgttc ctgcaagtaa tgcaataaga cctgacggaa aaataattct tctaccagag
      301 gtccaatgac acaagcaaaa acagctatca gtagtggttg ttcttgaaag gtactttgaa
      361 tattagactg atttagacct tgtccatcta gtcccacaaa ctgcttcaac atctctgata
      421 caaattcaaa gacaacagtc atcagaatga gaaagagcca tcctgttaag actccaaaga
      481 agaattttct tttagtcttt ctaatctcct tccattgttg gatcaatcta tccttgaaca
      541 agaaggagcc atataaagct aaaactacat aaacaagata atttgcaatg gcagatggca
      601 ttaaagaagc cagcaattta acgccatcaa acacaaagag gactgtatag agaatcacaa
      661 aaatcagaat tcccttattt ttgttagaca tcatttaaga catttccact cacaatcatt
      721 ctcgtggtca tcaactagcc ctgcagcctg tagaaaagac aatacggcga ctgggcctgt
      781 gaacttgaag cctcgttttt tgagatcttt ggctaatttc tcagataagg gtgttttagc
      841 tggggattgg cgataatcag gaacatcgtt aacgacagtt ttcccctcaa caaaagacca
      901 aagataggca tcaaaagagc cgtactctgc ctgtaactgt agaaaggctt gggcgttagc
      961 gcgtgtagca aaaatcttgg ctctatttcg aatgatagct ggattctcca gcatggcttc
     1021 caattcagtg tcagtcatct ctgcgactga gtgaatttga tagctatgaa aggcttctcg
     1081 gaaagcttgg cgtttgttga gtaccgtttc ccaagacagg cctgcctgat aggtttccat
     1141 acacaacaac tcaaacaacg cttggtcatc atggaggggc tggccccact cctcatcatg
     1201 ataggcgatg tagagtgggt tggtcatctt gacccacgaa caacgttttg tcattctctg
     1261 ctcctatttg accaacattt taagggccga cttgatatag ttctcagctg tatctgtcgt
     1321 tccttcaaag aatttcttga ttttcttgag ctctgttgcc ttgtagccca gagccaacat
     1381 ggcttccata gcttcttcca attcttggtt ttcagcactt gcttgcactg cgaccttggc
     1441 aggaaggtca tctcctgcaa ctactacctt gccttccaag tccagcacca tctgctgggc
     1501 tgttttcttg ccaattttag ggaacttggt caagtaggtg atgttcttgg tttcaatggc
     1561 ttgaaccaag ccagcattgt catcagcagc gataatagca agagctgata caggaccaat
     1621 cccagagacc gaaatcagac taagaaagag ctttttctca tcctctgagc gaaatccata
     1681 aagcaaatgg gcgtcctcac gcacaacctg atgcacataa atctgagcct cctgattaac
     1741 ctgacctgaa taggcataag gattggccac atgcaggata taaccaatac cattggtttc
     1801 aagaacaatg tatttggcag taattttggt aatgattcct tttaaatatg cgtacatagt
     1861 gttccttctc tcttatttcg tttatctagt atatcatatt ttctttatcc gaggagcgag
     1921 aatcgattat cacaaataca gtatatcaaa aacaatcggt atttatctgt tgacatattc
     1981 aaaattaagt actataatca tctgtaattg aataactatc ttcagggcag ggtgcaattc
     2041 cctaccggtg gtatagccca cgagccgaaa ggcatgattt ggtgaaattc caaagccgac
     2101 agtatagtct ggatgaaaga agataaaagt atatttgtgc tttttgcgtg ctctgaaatg
     2161 attacttgtc atttcagagc atttttgtta atcgcataag ttttcttttg tatgccttga
     2221 gtagtcccct attcaaggta tatttttttg gaggtagtga gatgagcgat tcaaaatata
     2281 tgaaattagc aataaaactg gcacaaaaag gggctggtta cgtcaatccc aatcctatgg
     2341 tcggcgcagt tattgtaaaa gataatcaca tcatcggaca aggttatcat gagttttttg
     2401 gtggcccaca tgctgagaga aatgctctta aaaactgtag agaatcccct gtcggagcga
     2461 cgctttatgt aacacttgaa ccctgttgtc acttcgggaa aacacctccc tgtatagatg
     2521 ctataatcga tagtggtatt acaagagtag tcattggaag cctagactgt aatcctattg
     2581 tatctggaaa aggagtaaag atacttgagg aaaataatct tcaagttact gttggaattt
     2641 tagaaaatga gtgtcttaac ttaataaaaa gttttagaaa gtatattacc cagcatgtac
     2701 cctatgtttt tatgaaatat gcaatgtcaa tggatggaaa aatagccact aaaacaaatc
     2761 aatccaaatg gattactgaa gaagaagcaa gaaagcatgt gcatcagtta cgacactatg
     2821 ttagtgcaat tatggtggga gtcaatactg ttattcaaga cgatcctttg ctgacatgta
     2881 gattggagga agggaaaaat cctatccgta tcatatgcga tacacattta cgaactcctc
     2941 ttacctctaa aatcgtaaaa acagcaaatg atattaaaac ttacattgcc acttcctctg
     3001 aagacaaaaa taaaatgaag ctatatcaaa atcatggctg tgaaatactt tccataaaga
     3061 aaaaaggcaa tcatatagac ttatcgagtt taatgcaaca tctaggaaac atgcagattg
     3121 atagcctagt tctagaaggg ggcagtctaa tgaattggag tgctttggaa caacaaattg
     3181 ttgatgagct gaaaatatat attgcaccaa aaatttttgg aggcagtgcc aagtttcctg
     3241 tcggaggtga aggcatttct ttgccaaatg acgctattag attgaaacct tatgcttttt
     3301 ctcaaatagg aaatgactat ctcatagaaa gtgaagtgat ttatccatgt tcacaggaat
     3361 aattgaagaa atcggaaaag ttgaaagaat acagaaagac tctcgtaatt gtaaactatc
     3421 aattaaagcc tcaaaaatat taacggatat ccatttaggc gatagtatag cagtaaatgg
     3481 tatctgtctt acagttactc atttcaatca tcaatccttt acagttgatg taatgaatga
     3541 aacatggagt cgaacagctc ttactctatt aaaacatgga agtgaggtga atctagaaag
     3601 agccttatct gtcaacggtc gacttggggg tcacgtcgtt acaggacaca ttgatggtac
     3661 aggaaaaatc tcgtcaataa aaaaagatga taatgctgta tggtatcaaa tcaacacaca
     3721 aaaagaaatt ttagatttaa tagttgaaaa aggatctatt acaattgacg gcattagtct
     3781 gactgtcgct aaagtctcca aagtaaactt ttcagtatct gttatccctc ataccttgaa
     3841 acaaaccatt cttaagagta aacaagtcgg gagtacagta aatcttgaaa atgatatctt
     3901 aggtaaatat gtgcaaaaac tgatggataa ctctccaaaa tcagaaatat ctaaggaact
     3961 attatatcaa aatggatttt agcagaaagg ataatcagtc aatggaatat cgaaaaatac
     4021 aagaagcatt agaagcattg cagaagggac gacttgttct tgttatagac gacaaggata
     4081 gagaaaatga aggagactta atttgttctg cacaagcagc tacaacagaa aatgttaatt
     4141 ttatggctac ttatgccaaa ggattaattt gtatgcctat gagcgaaagt ttagctaatc
     4201 aattaatgct ttcacctatg gttgaaaaca atacagataa tcataagact gcttttacag
     4261 tttcaattga ttataaagaa acgaccacag gtatttctgc cgaggaaaga ggactgaccg
     4321 cacgtatgtg tgtagctgaa gatataacac cctctgattt tcgcaggcca ggacatatgt
     4381 ttcctttaat tgcaaaaaaa ggtggtgttc tagaaagaag tggacacaca gaagcaactg
     4441 ttgatttatt aaaattagct ggactaaaag agtgtggcct atgttgtgaa ataatgaatc
     4501 atgatggcaa aatgatgaga acagatgatt taattcagtt ctcgaagaaa cacaacattc
     4561 cactaattac catcaaagaa ttacaagaat atagaaaagt atatgatcag ctggtagaac
     4621 gagtttcaac tgtcaatatg cctactagat acggtaattt caaagcaatt agctatatag
     4681 ataaactaaa tggggaacat catcttgctc ttattatggg aaacatagag gatgaagcca
     4741 atgtattatg tcgggtccac tccgaatgtt taacaggaga tgttttaggc tctttacgtt
     4801 gcgattgtgg acagcaattc gataaagcta tgaaaatgat tgttgagaat ggttcgggtg
     4861 tcttacttta cttgcgacag gagggacgag gaattggact tatcaataaa ttaaaagcct
     4921 atcatttaca agatcaaggc atggatacgc ttgatgccaa tcttgcatta ggctttgaag
     4981 gtgatttaag agaatatcat attggagcac aaatgcttaa agatctggga cttcagtcac
     5041 ttcatttact gacaaataat cctgacaagg ttgaacagtt agaaaaatat ggaattacca
     5101 tttccagtag aatatcaatc gaaatagaag ccaatcctta cgatagtttt tatttagaaa
     5161 caaagaaaaa tcgaatgggt cacattttaa atatggagga aaaataaatg aacacttatg
     5221 aaggtaattt agtagcaaac aatattaaaa taggtattgt tgtagcgaga tttaatgaat
     5281 ttataacttc aaaattatta tctggagcac tagataatct caaaagagag aatgtaaacg
     5341 agaaagatat agaggtagcc tgggttccag gagcttttga aataccactg attgcatcaa
     5401 aaatggcaaa aagtaaaaaa tatgatgcaa ttatctgctt gggagctgtc attagaggga
     5461 atacaagtca ttatgattat gtatgtagcg aggtatctaa gggaatcgcc caaatcagtt
     5521 taaatagcga aattcctgtt atgtttggtg tgctaacgac agatacaatt gaacaagcca
     5581 tagaacgagc tggcactaaa gcaggaaata agggttctga gtgtgcacaa ggagctattg
     5641 aaatggtcaa cctaattcgt acattagacg catagaacag actctacttt tagacgaata
     5701 aagaaacttg gcaaaatcgc aatttaatac tctgtgaaaa tcatagagca aactaggaaa
     5761 ccagtcgcag agtggtcaaa cacaacttcg atgttacaga caaaactggc aaaattagct
     5821 caaaacactg ttttgagatt actgataaaa ctgacgtggt ttaaaaagag tttcgaaaag
     5881 aattaggaaa acatgaagta aatatttcta tgataaaacg cataaaatca aggtttttga
     5941 acacctgatt ttatgcgttt ttctgatttt gaaaattttt cccagacttt tttatacttt
     6001 taatatttcc ccaactcacg gagactggtg tgattttcct gaatacgtcg gaacatcttt
     6061 tccatatccg acttggtaaa atgcaccaaa acaggacgtc cgtgaggaca gttataggga
     6121 ttgtcacatt gagaaagctg atagaggagt tgtctagctg aatgatcatc aatacgatga
     6181 ttggccttga tagatcgctt gcaagacatc atgatagcca gctctgctcg gtatttcttg
     6241 atagaaactt ccttggtcaa aaggagcatg tcgcacatct catagatgcc tgattcaatc
     6301 tcttcttctg ccatccaaat aggatgttca cgtagaataa attgattttc tccgtactct
     6361 gctagaaaga cgcccacttc ctctaagaga ggcattcttt ccttgagacg cagggcatca
     6421 tccgcaggaa attcaaagat atagggcact aggagttgct gctggctctg gtcaacattg
     6481 ccaatgcttt cacggtactc ctcgtacttg acccgttcct gagcagcgtg ctgatctatg
     6541 atgtaaagtc catctcgccc ttgggcaaag agataagtcc cgtgcatttg tccgaaaaac
     6601 tccaactctg ggaagctgga tgcttcttct cgctccagtt tgtcataagc cttatcgatg
     6661 ctagcaagat ctaactctgg atggtctagc tggtcgtagt tagcaggctt tctctctgca
     6721 aaatgcagtt ttgctggctt ggtcaatcgg tccaaggttt ccttggcaaa cagggtcaaa
     6781 tcctgccctt catcagtcaa ttctacctga taatcagcta cttcagtttg actaggtctt
     6841 gacggctcag ttttctcata gtagagcgta ttttctttga gtgggagaat agtttgctcc
     6901 accttctcac gattgcgcac ggtcgatttg gcaagatttt ccaaggcatc tggaatcaag
     6961 gtttgttcct tgagactatt tgcaatagct tctgaaacca gggtcatcag ttctttttcc
     7021 ttggaaatcc gcacctcttg cttagttgga tgcacattga catccgctag ataagggtcg
     7081 atatggatgt gaatgacagc cagtggaaaa cgtccaacca taagcttgct tccgaaacca
     7141 tccaaaatag cacgattgag caggaagttc ttgatataac ggccattgat gaagaggctg
     7201 atataattgc ggttagcccg agtcaactca ggtaaagaca caaaacctga aatttcgaaa
     7261 tctaggtcag agttctcaat ttcaatcatc ttcttggcac tgaccaaacc gtaaatccct
     7321 gcgattgctt ggcgcaattg accagtccct gctgtccgcg tcatttcctt gccatcacta
     7381 atcaagctaa aagaaatctc aggatgggcc aagcccagac ggttgacaat atcaatgata
     7441 tgagacaact ccgcttgctg gctcttcata tacttgagac gggcaggcgt gttgaaaaag
     7501 agatcctcca cacaaacctt ggttcccaca ggactagtcg ctgggatgac ttcctcaact
     7561 tcacccccac gcgcgactaa cttggttcca tgactagcac catccaccgc cgttaacaga
     7621 gtcaagacac taacagacgc aatagaaggc aaggcttcac cacgaaaacc aagcgtccga
     7681 atccgaaaga gatctgcttg attttttatc ttactggtcg catggcgacg cagggccaac
     7741 tccacctcat cgtgggcaat tccatgaccg ttatccgtga tttgaacctt cttgagacca
     7801 gcttcctcaa tctcaatgat aatctgacta gagcccgcgt caatggcatt ttctaccaac
     7861 tctttaacca cactggcagg acgttcaatg acctccccag ctgcgatttg gtttgccagc
     7921 atctctggca attcaataat atgagacatc tttcagcccc tttctgtatc tattttccta
     7981 gaaattaatt agtgctatta taccatattt cagaaagaac agaaaaactc caccacatag
     8041 gagcccaatc cctccacata gacaaagtcc cttgccagag ggagtaaaat agtggtatac
     8101 tcaataaaaa tcaaagagca aactagaaag ctagacgcag acagctcaaa acactgtttt
     8161 gaggttgtag ataaaactga caaagtcaac aactatccta cggcaagacg acgctgacgt
     8221 ggtttgaaga gatttttgaa gagtattaat aaaggtgtac aagaagtgag attaattttg
     8281 tatgaaaaac aaggagagaa atttatgaaa gtagaactac agattagtga gacttacgag
     8341 gaggaaaagc tgattgtcca agcacctcaa gaaaccgaaa aagtccagaa agtcatcgag
     8401 ttcgcagaaa atctggacca aagagaaaaa atcaaaggaa aaatcgatga tcaggtctat
     8461 ctagttgaga ttggcaagat tcagcgcttc tatatcgaga atcggaaggt tctagcagaa
     8521 accgcgagtc agacctacag cattgatttg cgactctatc aggttcttaa actcttgcca
     8581 agcaatttta tccaaatttc ccaatcagaa atcatcaata tcgattccat ctctcacctc
     8641 aagctcacgc caaacggtct ggtagaaatt ttcttgaaaa acgaaagctt cacctactct
     8701 tcacgccgtt atctaaaaac catcaaggag aaattagaac tatgaaaaaa caagtatttc
     8761 acgatgcagc tgccggtgtt cttatcggcc tcatcctctc catcctcttt tcactcattt
     8821 atgcaccaaa tacctacgca ccactaaatc cctactctct catcggccaa gtgatggatc
     8881 agcatcaggt tcacggtgcc ctggtcttgc tctactgcac acttatctgg gcagccatcg
     8941 gtatgctctt caactttggc aaccgcttat tcagccgtga ctggagcatg cttcgtgcca
     9001 ctctgactca tttcttcctt atgctggctg gctttgtccc actagcaact cttgctggtt
     9061 ggttcccttt ccactggatt ttctacctcc agctcattat cgagtttgcg attgtctatc
     9121 tcatcatctg ggctattctc tataaaagag aggctaaaaa agtagatcac atcaatcaac
     9181 tcttggagca tagaaaatag gaacctcata ataagcaaat ccgagaaaga aacttctcgg
     9241 tttttcttta tcctgctgca ccttactata gtgaaatgaa ataagaacag gataaatcga
     9301 tcaggacagt caaatcgatt tctaacaatg ttttagaagc agaggtgtac tattctagtt
     9361 tcaatccgct atatcataac agttatttca gatcttctcc caaatttcaa gttgaagtta
     9421 gccacccaaa agaaattcac ttggcgactt gtagtccaag cattttttag ggtagttgtt
     9481 aatccaattt tcgataaaag ctacttcttt cggagtcgtt ttcttggttc ctttaggtaa
     9541 ccatctccga attaatcgat tatgattttc atttgaacct ctctcccatg aagagtaagc
     9601 atgtgcgtag tagatatgtt cctcaggaaa tacctcagac aatcgtttga actccgaacc
     9661 attatctgca gtaatggaca gaatacgatg ctcccctaat agtaacgtaa gcgcctgatt
     9721 gacagattca gcagttttat ttggaattaa ccttataatt tggtgtctgc tccgccggtc
     9781 ggttaagact aacaggcaat aattttttat cttagtcagt aggacggtat caatttcata
     9841 atgaccattt tccaagcgaa gattaataac gtcaggacgt tcttcgatag atttacctgc
     9901 cggcttaaag ttcggactag cttgcttctt gacacctttc ctttttctgg gataaagcat
     9961 gtcggccttc gtcaatccta aatgaccatt atgaaaccag tagtagatgg ttgaaatacc
    10021 aactttcact tgcttcttgt taaccatcat ttcaggcgaa aatttttgct tatgatagtg
    10081 taagatcttc tctctgattt cctttgttaa aattaacttt ttcaccgacc gttttcgatt
    10141 gaattggtaa acagtttgtg cgtaatcggc agaatagacc tttttgtata gcccttttct
    10201 cacttgttgt aaagttgtac ctcttttgac ttcagtatga atcgtttgag gcgcctttcc
    10261 taacaagcct gcaatttcac gattggactt atttttattc ttccaccgct caatcaagcg
    10321 acggttatct attgtcaaat gtttcccttt tggggtataa tgttcttgca tctctgtgcc
    10381 tttccttgtg tttgtggtga acaacaagta tagcacagag gtgttttctt ataccttaaa
    10441 tcacatttca tttgacaaca ggaacccttg aacctgtttt ttcagctaaa ccaaggctat
    10501 gattagcctt ggccacctag cctaacagtt tcaaacctgt tatcaaatga aatcatcaac
    10561 ttactcaaac atagtgaaac atctaattct tgactaaggt aaggtggcta acttcattat
    10621 agaactttca tttcagattt tctttgtaaa ttttctatct tttttcaaaa tattcttgca
    10681 tttttccaaa attttttgta gaatatagat tacatataca gattattctg aaaattcaaa
    10741 aaggagtatt tatcatgtcg caagctattt ccttaaatca atcaacctgg gcaagcaagc
    10801 taaaagcaat gggacctgga atcctaatgg caactgccgc tgttggaggt tcccacattg
    10861 tatcctcaac tcaagctggc ggttcttacg gttggtctct acttctcttg gtcatcttag
    10921 ccaatgtctt taaatatcca tttttccgtt ttggtgctga atacacagct gatactggaa
    10981 agactttggt tgaaggttat gccgaaaaag gaaaactcta tctctggatt ttctttatcc
    11041 tcaatgtctt ttcggctatg gtcaacacgg ctggtgttgc cattctgtgc tcagctatca
    11101 tcgccagtgc tttcccaatg attggactta gcattactca gtggtccctc attctcgttg
    11161 caatcatttg ggctatgcta ctctttggag gctacaaact tttagacggc atggtcaaat
    11221 ggattatgtc tgccttaacc attgcgactg ttcttgcagt tatcattgcg gcggtcaagc
    11281 atccagaata cagttctgat tttgtcgaga agacaccttg gcaaatggca gctctgccct
    11341 tcatcgtctc cctcctagga tggatgccgg ctcctattga aatttcagcc atcaattcac
    11401 tttggtcagc tgaaaagaga aagaccgtca actttaacac agaagacgct ctgtttgact
    11461 ttaacactgg ttatattgga acagctatcc tagccgtctt ctttgtggca ctgggagcac
    11521 tgattcagta tcctacaggg caggcggttg aagctgcttc agccaaatac atctctcaat
    11581 tcgtgggcat gtatgcctct gttcttggcg aatggtcccg ttacttgatt acctttattg
    11641 ccttcctctg tatctttgga acagttataa ctgttatcga tggctattct cgcgttaatc
    11701 aggaatctct ccgactgcta atcagtcaaa aagaggacaa tcgtaaatct ttgaacatct
    11761 ggatgaccat cactgctatc atcggtatcg tcattatcaa gttcttcgct ggtcaggttt
    11821 caaccatgct ccgctttgcc atgattggct ctttcctgac aacacctttc tttgctcttt
    11881 tgaattacgc cttggtaacg cgtgaaaaca aaaatcttcc ttcttggctc aaacaccttg
    11941 ccattgcggg attgattttc ctctttggct tcgccatctt ctttatctac gcactcgcaa
    12001 tcggaaaagc agggtaaggg acaagcgcga gatgaagata aggtttcatt tcaagagaaa
    12061 attcagcaaa tatttctatg ataaaaagca taagaacaag gttttgaaga cctgaactta
    12121 tgctttttta cgttcttaaa gactgtttat actcaaaaaa cagttgaaca acttcaacca
    12181 cctcttataa gaactttata ctattcgaga atctcttcaa accacgtcag ctctatctgc
    12241 aacctcaaag ctgtgctttg agcaacctgc gactagcttc ctagtttgct ctttgatttt
    12301 cattgagtat taattctcct tttccaactc atacaaatct gcgataatag ctgcgacatg
    12361 tttgatatct tccagcatgc ctcgcatttc aaagtcagcc aatacaggga agccaaagcg
    12421 ttgactgtat tgcttggctg ttaggcagta ttggttatta aagttacgat ttcctgaccc
    12481 aaccacacca aaacacttac tagcattgtt accataggca ataaaatctc ccaccggtgt
    12541 cgtcaaaatc tcaacatctc cgttatccac gccattccca ccttcgagat aggtcggcaa
    12601 aaaagcgaca tagggatggt ccatttcata gaaatttttg ccttccttga ccaaatcctt
    12661 gatatgaatc ttttgaacct caatcccttt gtactgggac aagagatagt ctttcaagcg
    12721 cgtcacaaaa ctttcagtgt tgccactcaa actaatataa actaaagaaa ttgtcttcat
    12781 atgtacctcc atgattttat ctataatcaa aaaacaacta ccaagattgt atcttgatag
    12841 ttcttttttg tcaatactta ggtaagctct tctgtttcct cttcttctgc caaggctttc
    12901 tgttgtcgcc acatcttata gaagacaagg gctaggaaga gaacattgat gacgatatag
    12961 aaaatgctaa cagcttttga accaatactc actgtcaaac gcatgctctc atcttgaacc
    13021 aactgcacag cgtcttgtaa accaacatag ccataaatcg aaccaataat agctaacaaa
    13081 acatagccaa cgtaagtata gtttgcatat tgcaaatttt tacgaacaag gaagacaatc
    13141 gctacgacca ctaaaatagt agatagcaca atcaaagcaa cattaaaaat agaatgagat
    13201 gcttctgcta ctctataggt ataattaagc tggtcttcta attgctgagc attgacccct
    13261 gcaacttgtt tctgagcagc acgaagggct tctttactag gcaagggact cacaattcca
    13321 aacagagacg gaactgaaat aagggcagac aagattagca aaacccatag ataaattggt
    13381 ttctttttca tgataggacc tcctgataat attatagcac cttttgtaaa ggaatacaaa
    13441 tcttatagta tagtttctac ctgttttcgg taggcttttg gcgattttcc gcacaatttt
    13501 cggaaaactt tatagaaata tccaaggtta ctgtaaccaa cagcataaca aatatcttcg
    13561 atgctgtcac tagttgaaag caagagctgc tgggcagcct taatacgttg tttatttagt
    13621 aactctgcaa aggtcgaatc ggtttcacgc ttaatcaact gccctagata gacaggattg
    13681 ataaagaggg ccttactgat atccttgagg gaaagctctt tttggtaatc acgaccaatg
    13741 acttccagca cactgaccac attttcattc atacggtatt gatcgaaaaa gctgatcaga
    13801 gtttccttga tataagaaac caattcatcg aaggattgaa tagcatgaat ggttttgaca
    13861 atgtccgtca tatcatcagc tttgagatgt tcaaataaat ggaaaacatc catgacaaac
    13921 tggataaaaa gctgtttagt aatcgaaaca cgaggtgtat tttcaagaac taccttctcc
    13981 aagagattta actcttctac gatttgattg agatttccct gaatgataac cctataaatc
    14041 ggttcgtaat aggcaaagag actctcagac tgctgcagat ttacagaacc gtaaaagagg
    14101 gttttctcag cattcagctt ccaacgttca aagtgttctt gataaggagc ttcaaaaggt
    14161 gtaacgacta aaccatctag gggttggtca gaaatgaaaa tctgccagtc ttggcccaag
    14221 acatagtagg gaatggcgaa ggaaccttgt ttttccttgg atagacctat ccaccaattc
    14281 tccttatccc ctaaataact aacaaatcca gcctcgtcta attcttgact aagagtctga
    14341 cttttcttcc ctctctcgcc gagctgacct gcaatcttct ccagcagatt tcccagctct
    14401 accttatcaa caggcttgac caaatagtcc accacactaa ggttcattgc tctttttaca
    14461 taatcaaact cctgataacc tgagagcagg atataggcag catctggtaa gatctctttc
    14521 atctcccgaa tcatatcaag ccctgttttg tctggcatat tgacatcgga aatgatgaca
    14581 tcgacaggat tttcctgaac atattctaga gcttcatcgg catgactggc tgttgcgacg
    14641 acctccatat cccacttatc aaagggaatc aaacgcttca gaccttctgt caccatgtac
    14701 tcatcatcta ctaataatac tttatacatt ttctcccttt ctactcatct tgaattgtaa
    14761 tacgatactg aacaccggct tgctctgcag actctatcgt aatggcatag cggtctccaa
    14821 aatagagcac aaaacgctcg tgtacattga caatcccgat agactgcctt tgatcactgt
    14881 agctggcttg gtgttcaaaa tatctctgac ttaatttttc tcggatattt gccaactttt
    14941 cagccgacat tcctctacca ttatcgacca ccaaaatttc cacaaaacca tcctgtttaa
    15001 gagccttgat gctaatcaca ttatctgtcc gcctgtggtc aacaccatgc gcgaaatagt
    15061 tttctaccag cggttgcaag gtaaacttgg gaatcttcat attctctaac tctggatcta
    15121 tcttgaaacc ataggcaatg gacttgggat agcgaaccat gcagagatag ctgtatttac
    15181 ggcaaaattc taattcctgt ttgaggaggg tctctctttc gtcggaaata ttgttacgca
    15241 agagactact gaattcataa atgatatctg ccaactcatc ttgactctgc atgactgcgt
    15301 acatgcgcaa gaactccagc gtattataca tgaagtgagg gttgatttgc gcctgcaagg
    15361 cccgcatatt ggcatctttt tgactgagtt ctaactggta aatatcatgg atattctttt
    15421 ccaatcgatc caacatatca ttggtcgtct ccgcgattag gagcaattcc tgatcctttt
    15481 caagcgtatc aatgcgaaga ccttcttgtc cttgggcaat agcttggatg gaatccacca
    15541 aatccactac ctgcttttga taattagcaa aggtctgcct caaagtcaga taaagaatga
    15601 caataaagag aaggctcaag cccacaatca aagcagagct ggtcaccgtt ccttgtaaaa
    15661 caaagttttt gggaactgcc acctgaacct gataaccatg agaagttaag ccaactagcc
    15721 aattttcact ctccttatca actgtctcac caatatgaaa aatctccgta tcaaaaggtg
    15781 aggtcactgt tactgccatc ggagtatgac ctctagtatt atcaatggca tggtataaaa
    15841 cagcaggatc caaggagatg taaatcactc ctaagtcttg atctgacact ggatctgaca
    15901 ctggaatggc aaaactattt cctgctggtt tgaaatcctc agccctgatt ttttctccac
    15961 tacgtttgtc tttagtagaa acatagactt ccttgtaatc ttgaaaagca atggccactc
    16021 cagttacaaa atcatttcga acatagaggt catcaacatt ttcatacaga gagactgata
    16081 tataaggcga agctttccgc tctaattgcc agtaaaaata gtctggcatg ctaaggctaa
    16141 agtatttata gattccctca atacgagctt gattttcaac taaagcctga gctatctggg
    16201 ttgactctct atggtaatat tcaatttcac tcactgttct agcagttaca cgctgggcta
    16261 ctctctgggc ttccttttca cgtgaatccc agtcagcgta agagagtaag attgcaaaac
    16321 tcgcaatgat aatgatcatc actgaactat aggttcttag aagcgtatgt agccaaaagt
    16381 gcttcattct attttgtcgc caatttttca tggatgattt catagacagg ttccaacata
    16441 tcactgataa agaaatgcca catcccaatt cctacaaaaa atagcagggc aggcatatag
    16501 taaccaattg ctacaagtag cacagtccca aggagaacct tggccacagc tgctagactc
    16561 ataaagatgc caatcaagga taatttgaga ctatttcgat aggacaaggc aaaataaact
    16621 tgtaatttta aagatactgt ataagccaaa aagatcaggg caactactag gacattcaaa
    16681 agggtcgcaa tcaaatgaac aatggtctga tgaggcaatt gcaccaagag atacaaacca
    16741 taggtcaaaa ctagacccac acctaaaaag ctatagaaaa tcaggttgct tgagacaaaa
    16801 ttttgcttgt aaagagacca agcctccttc aaactgtatt cccgaaagct ataaccatgt
    16861 tctgcatata agctcatcaa ggtggcacta gccggcgcta gaccaaggat aatccctcct
    16921 gctaaagtta atagccaaaa gaaggctgtc gcaatcatcc ctaaaaagaa acgattaaag
    16981 acaaagtcta aaaatctacc catgatattc ccctaaaaat aactatgaaa ccattatatc
    17041 atatttcaag cgttttcaaa aacttctagt atccatttac aatcccacca aaccatggta
    17101 aaatgagaat tgtgaaaaaa ttatcaggag acaattcatg aagaaatcta tcttaactac
    17161 actgcttttt gcagttcttt acttcctctg catggggatt ggtgtccttt tgggcaatct
    17221 ctttgaccaa actggaaaca tgttttatgc gcctgccttt actgcccttg tcggcggtag
    17281 cgtctatatg atcctagtcg caaaagttcc gcactttgga gccattacca ctatcggcct
    17341 tgtcattgcc ctctttttct tgggaactaa acacggtgct ggttccttcc ttcctggaat
    17401 tatctgtggc ctcctagcag atggagtagc tcatttagga aaatacaagg acaaaacaaa
    17461 gaacttcctt tctttcatta ttttcgcctt tagtacaaca ggaccaatct tgcttatgtg
    17521 gattgcgccc aaagcctata tggctactct tctggcaaga ggaaaatccc aagaatatat
    17581 cgaccgtatc atggtcgctc caaaccctgg aactgtcctt ctatttatcg caagtattgt
    17641 catcggagcc ctagtgggtg ccttgattgg acaagccttg agtaaaaaat ttgcccagaa
    17701 aatctgatca gttaaaaaga gccacgcggc tctttttatt tatgactcaa tttcttagtc
    17761 aagaaatctc ccaagaattg gattgcaaag ataatcaaaa tgataataat ggttgccaag
    17821 atggtcacat cgtgattgta gcggttaaat ccataagcga tggctacgtt accgatacca
    17881 ccagctccaa ccgcaccggc catagctgtt tccccaacaa gggaaatcaa ggtcacagtc
    17941 gtcacacgga tcaaatctgg aagaccttct gataggtaaa cacccacgat gtcccagaaa
    18001 gtcgctccgc tcgcttgagc cgcctcaatg acaccgccat ccaattcagc caagacaacc
    18061 tgcacctgac gggcaaagaa ggcaaagact gcaaaagaaa gtgggacaag ggctgcattt
    18121 ggcccgatac ttgttttaac aatcaagtga gaaagtggtg acaagattgc caagaggatg
    18181 ataaagggaa ccgcacgaaa aattgaggta attttgtcta aaatccagaa tacgacttta
    18241 ttctccaaga caccacctgg cgctgtcaag acaaggaaaa gacctgccac tagccccaag
    18301 aagcctccga tgataaagga aagaactgtc atataaagag ttaagtagat agccgttccc
    18361 cagcctgcct gaccagccca acccatttta tagacatttg gtaaataggt ttgaatcaat
    18421 gattccatct tactgtactc ccttcaatac ttttagttgt acacctgctt gacgaatggc
    18481 ttcttgggca cctgccaacg ctgctttttc acctgacaag accaccacca attctccaac
    18541 aggagtacca tcgagaattt cgatattccc atagagaata ttagccatta cttggtaatg
    18601 cttgtacaat tcattcaaaa gtggctcgtc tgttgaagct ccagcgtact tgagttgcac
    18661 caagagactg ttttcagaca agtgttccac gatttcttgc ttctcgattt tgaccatggc
    18721 ttcgtcaata cctgtagctg ttgagataaa gtcttgagtc aaaggttgtt tagggtctga
    18781 gaagatttca agcacactac cctcttcaat caaatgccca tcctgcataa ccgccacacg
    18841 gttggcaatg tctttgacaa tctgcatttc atgcgtaatc aagacaacag tcaagcctaa
    18901 tttttggttc aaatcttgca acaaggccaa aatctgcttg gttgtcttcg gatcaagggc
    18961 agaagttgac tcgtctgaaa tcaagatttt tggatcattg gccaaggcac gcgcaattgc
    19021 cacacgctgt ttttgccctc cagatagttg tgaagggtag ttttcagcac gatctgccaa
    19081 accaaccaag tccaacaact tagctacttt agccttcttt tcttccttgc tgagtccaga
    19141 gtgtttaagg gcaaaggcta cattctcctc tgctgtcttt tgactcatca ggttaaaatg
    19201 ctggaaaatc atcccgatat cttgacgttt acgacgcaac tgctctgccg tcaaggtgac
    19261 cttaccatca aaaatcacat catcgtcaat ggtaattttc cctgcagatg gtttttgcaa
    19321 gaggttaatc acccgtacaa gggttgattt ccctgctcca gaatatccaa cgattccgta
    19381 gatatcccct tcttggatgt gaatggtcac atccttaacc gctgtgatgg ttctcttctt
    19441 ttggtgaaaa gtcacatcga tctgatctaa cttgataata tctctactca tagcttctaa
    19501 tcagctcctc tactaattcg atatgggtgt aataatcagc gattcgcaca ttttcatctc
    19561 caccgtggtc tcggctattg gcatttccta gaccgaatgc aaccattggt acctctaggg
    19621 catcaaagac cgtatgcata ggtcctgtcc ccgctgtcgt cggcaagact gaaacgccct
    19681 gtggatagaa tttcttggcc aactcgatca cattgagaat ggctggtgcg ctcatatcgc
    19741 ttcgatagct catctctccc aaggtatagt ataattctac cttatcaaag ccatttttgt
    19801 ctagctgttt ccgaattttt tccagaacat catgcggttc taggcccgga accagacgaa
    19861 cctctagctt ggcactggct tctgcaggta aaatagtctt aacaccctga ccttgataac
    19921 cagactggat tccttcgata ttaagcgctg gctcgaaaaa gaaacgtttt agaaaggcca
    19981 tccgatcctc ctgtaagaga ggcaactcca atccataaat ccgactaact tcctctgggt
    20041 ttcgttgacc ataagtttct agcaaggcca tttctcgttc attaggctct tgtacttctt
    20101 cgtacaagcc ttcaaccaag atacggccat ccgcagcacg aagagactgt aaggcttgga
    20161 ggagatacca aggagctgat tccacaacac caccataact cgagtggata tccacatcag
    20221 cgctttttac cttggcatca aaggtcacaa tccccttatt gccaccagaa atttccagct
    20281 gttccaaggc atttttggtc ccttgttccc agaccaacaa atccgcccca cggagtttgt
    20341 ctgcatgctt ttccaaatac ttatctaggt ctgttgaagc cgattcctcc gctccctcca
    20401 tgataaagct gatattgaca ggtaaatcat catggtgctg catatatttt ctcaaagcac
    20461 tcaagcgagc tgtgatatga cccttgtcgt catcaacccc acgcccatac atgaagccat
    20521 tgcggaccga aagcgtaaaa ggatcctctg tccagacctg atccccatcc gctggcacag
    20581 tgtcatagtg gttatagaaa atcaaggtct tggcatctgg acgcgaactc ttgaaatgtg
    20641 ccatgacaaa gggcgctgta tagctctcat caatctccac ttcagctcca acacgcttga
    20701 aaatctcacc cagataattt gcgacttcct tgagtccaac ctgctgggca aagactgatt
    20761 tcttagaaat caaggtacgc aaaacctcaa aataatgttg gactacatga tccttttcaa
    20821 atttttcaat ctgttcttgt tcgctaggaa aaaccatatt ctctctacct ttctatgaac
    20881 gaatagtctt ctttacaatc tatactctat acaaaagcaa gaggtggaat tttctctccc
    20941 acctccctgt ttcttattac caaactggtt gatccaaacc atctgatgat tcttcgataa
    21001 cttttttcac gtcatctgtg tggtaagctg cgattacttt cttgatagca tcagccttag
    21061 gtgatgtttc ccaatctttt tttgcaacaa tgatgttgta ccattgtttt gagttttcat
    21121 cagcttgttc tttgaaaagt gctttcttgt agtccaattt tgcttctgta acgaaggtat
    21181 tgtttacaac ggcagcgtca actgatgaca atgaacgagc tgtttggcta gcgtccaatt
    21241 cagtgatttt caagttcttt ggattttctt tgatgttggc aactgttgca agagcagttc
    21301 cagaaacatc caatttaatc aagccagctg attgaagcaa gtaaagcgca cggctttcgt
    21361 ttgtagcgtc attcggtaca gcgatttctc cgtttgctgg gatgtcttct actttagtgt
    21421 acttgttggt acttccattc aaacctgagt aaaggcggat tggagagatg taagtatctg
    21481 caatcgctac aaggtctttt ccgttttctt tgttccagtt gttcaagaag ttatagtgtt
    21541 ggaaagcgtt caaatctact tcgccatcag cagttgcctt gtttggttgt gagtagtctg
    21601 tgaactctgt aaattccaag gtaattccgt cttttttaac caattcttgg attttgtccc
    21661 aacgtttttc ttcagaaccg ctacggttaa cagttgcgat tttgatagtt gttgcattgt
    21721 ctgctttctt ttctgagttt ccgcaagctg caagagccaa acctgcgact gtagcaaggg
    21781 ctgctaggcc aagccatttt ttgattttca tgattctttc tccttaaaaa taataccgta
    21841 ctagtatctc acaatttgtt tgatttcaca aattgttatt agataatcag atatatagtt
    21901 taaaactata tccctaaaaa ctgagaaacc acccacctta ctcagaatgg atgatttcaa
    21961 gaaattattt aatatcagct tctgccggta gataagtgtc tccgaagaat tgtttagaca
    22021 atttttcaag agttccatct ttataaagtt ctttgatgcg tttgtctaca aatgatttca
    22081 actcatcttg accttgagca agaagtgggt aaacgtacgg ttgttggtcg cttggaagtt
    22141 cgataacttt caagttgtcc aaaccttggt tcttgatcac tgtttcaaca ccgattttat
    22201 caaaaatctt gtagtcaaat tgtccatcgc tcaaacgtac catgatttgt tggaagtctg
    22261 ccttagtata gttaaggata gttgggttgt ccgtgtgttc agcattgtat gcttctaact
    22321 gcttagctga tgtagtggct tgaacgactt ccgtcgattt tccaccgata tcatcgagag
    22381 acttgatact agagtcatct ttcttcacga caaggacatt aggattttgg gcaattggtg
    22441 cggcatagag gtatttctcc gcacgttctt tagtgtagct aagattgttg acagccatat
    22501 tgtaacggtc agcgtcaaga ccagcaaaga cacctgacca ttctgtcttt tcaaacttga
    22561 catcatattt gtcagaatct ttaaagatag cgcgaacgac ttcaatctcg taaccagtca
    22621 attcgccatt ttcttcatag ataaatggcc ttggtgatcc attggttgca acgatgattt
    22681 ctttcttgct agctgcttct ccttctttct tagcaccccc tgagcaagcc gcaagcacac
    22741 ctgcagcaac aagtcctagg gcagcaagag atgagtattt aacgattttt ttcatgtcat
    22801 ttcctccaaa atagaatacc ttataatctt aacagaaaaa gagcatttac gccattatat
    22861 gatatctatc tctgtgataa gtttttttat gggtaattta aaagaccaaa cgcaagatgg
    22921 caatcaagac cactccaaag agaactgttc cgactagatt gcggtagcga aaggctaccc
    22981 aagctgttgg aaagacggct aagaagtcta gccatttaat ttgaggaagg ctcccaacct
    23041 tacctgtcac tacgcttgaa agaatcaagg caaagataat ggaaacgggc aagaacttca
    23101 aaaaacgctc aacgattgca ggcaagccct tatacttgac taagatgaag ggaatcatac
    23161 ggagaatcca agtcaccaag ccagagaaaa taactgctaa taaaagatac ttactgacca
    23221 tctaaaacca cccccatgct acaaccaagt agcgtcgcaa acagaacagc tagtgactga
    23281 gacaccactg tcaagagcaa aaagaaggac accgcaacaa ctgctaggat aatgagcaga
    23341 ttgcggacag gaatccgtct ttgcatcatc tgaaattgcg aagcaaaaat accaataaac
    23401 atcccaacca gggcaaaatc caagccaaag atttctggat ttggtagcag gccacccaga
    23461 tccgttccga ctactgtccc cacaaaccaa gccacatagc tgttaagatt gtttccgtgc
    23521 atccacatag gatttacctt gtctgtatgg gccaattcac ccatcaaaac gccataggtc
    23581 tcatctgtca agatactaga cataccgata ttgtaccaaa gactggtatg acggaaataa
    23641 gtcgatgcgt gtaaactcaa caaaaagaga cgcaagttga ttagaaaaac cgtcatagca
    23701 atagctgcca caggagcttg aaccacaatc agtgccaaca tggcaaactg ggcactccca
    23761 gcataaacaa agagactcat caagcccatc tcaacaggtg tcacataggg cgcaccgata
    23821 gtcccacagg ccaggccgat actgacatag ccaagagccg ttggcatggc tgcctgcgcc
    23881 ccctcctaaa atcctttttc tttcatcttt ctcctcatat tgtcttaata atactcaatg
    23941 aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaaacaccg ttttgaggtt
    24001 gtggatagaa ctgacgaagt cagtaaccat acctacggca aggtgaagct gacgtggttt
    24061 gaagagagtt tcgaagagta taagtaggct gaaaagaatc cagtcacagc atggactatt
    24121 atatagcaga ttgaaataag atgagaacaa atcgattggg aaagtaaaat taatttctat
    24181 aaatgtttta gcaattgttt catactattt tagattcagt ttactataac acattcagaa
    24241 aagagaaaaa gtctgttgat tttgaccatc ataaaaagac tggcaatcca gtctcaaaca
    24301 tatattatag aaattctcca ctaaatactt tcacgaatat tcagaagcat aacaaaggca
    24361 actagaagaa atagcaataa aacaaagcta actgccagag ttccaaagct agtagcaatg
    24421 gttaccaaag ctattgtaaa taagctaggt aaaacaaccg taatggcacc gatagaggat
    24481 tgaactgctc ccattgactc ctcaggtatt tgtttaaaaa cgagttcttg caatctagga
    24541 gagagaacac ctgcgaaaaa ggcatccaag gtactaaaga tgagaatcca gtcaaaacga
    24601 actgtggcaa atcctactag aagaagcaac tgggtgacaa gtgaggcata gagagctgtt
    24661 tttatagaaa tcgtatgttt cagatagcca cttacaaggc ttccgacaat cagggctgac
    24721 agttcaaaag tggatagcaa ggcaagagat tgacctgttt gtaaattcaa aaagggttga
    24781 ttcctcaaaa atagagtaga gacaggaact gtaacattta tcactgcttg actagtagag
    24841 ataataaaca aaaccaagag caccttattc atattccata tcaatttcga tgattggagc
    24901 aaatgctggc aaaaggattt tacagagagt ccttcttgat agttaatcgt tttttctact
    24961 ttcaagaggt cagtttttat gaagaggata cctaaaaatg cgattaaaaa ggtaagagcg
    25021 ttcagcaagg aaataaactg gatggataga atacctaata agactcctcc tagaatatta
    25081 ctgattgttt tcactaaact aacagttgac tgtttaaatc caatagcttc tgccagatgg
    25141 tcttgcccaa taattctaat gaaaatcgga gtgagcatgg cgcctgaaaa ataactcaac
    25201 gtgtcagaca agaggttaat cagacaaata aatgctacta ataacaagga aaaggactgc
    25261 cctgaaagtg ataaagacac tatagagtaa agcaaaaatt ttgcaaaact aatgactgtg
    25321 tatttcaaga cacgatgatg ttgaaaatcc gccaaaactc ccagaaagat ttgtagaact
    25381 tggggcaggg tttctgaaat cgtgatgagt aaaatcgcca aaggggcaaa agatgcatct
    25441 gccacataat tcaggaaggc cagataaaaa atcgtatccc caagcgttga aatccactgg
    25501 ttgatagtta attgcctaaa atctctattt tgaagaaata ctttcatcac aactccttct
    25561 taagttcaaa tgggaatctt tccccaagga tagaccgcga tactactaac aaccaaaatt
    25621 acagtaacat caaaagctga ccaatgccat tgtagactat atgcagtcca ataggccaat
    25681 aaattgactt tgtcattcta aataagactg caaatataag acctccaccc atatagaaga
    25741 caaagtctgt caagacccaa ccgtgattac taatgtgcga gaccccaaat aaaacagcgg
    25801 aaccaagtac atctagcccc catttctttc ctttttccag agcagtcatc actaatccac
    25861 gataaatcat gtcttcaaaa atgggacctg caatcacagg ataaaaaaaa tacatcaaaa
    25921 atgccgtagc tcctgtaaaa gtaggagcag catgttgata agaaatttca tttcgagtag
    25981 gtgggaaaag aaaaaaggtg acaaaattcc aagcaacaaa agcaagcaga gctaggaagg
    26041 aatagaaaag ataggatcct ttaaactttc tactattgat tttctgccat ttccccgacc
    26101 aaatcatagc aataagagca aataaaacca caagaaaatt caacatcata tccgacagat
    26161 aataggcaaa gtcagatagc ccagcaacaa ggtcgctgcg taaaactaaa acactgaact
    26221 tctggtcagc aataactagt agaaaaacta taataaagta gcggtgtgag attatctttt
    26281 tcattctatt tcctcctata catatgaaat aaagacagcc cctacccaag aaaaggcaag
    26341 ggctttggat attaataacg acggcggtca gaatcaaagt caggtctatc gttaaaattt
    26401 ttcttccaga aattagtgat tgaccaagac gttcctaaac ctccccaact accacctggg
    26461 ataacggcat tatacccaag ttcccctcca taaactgtct ccaactcagc ttctgtcaat
    26521 tccattgttt ctgcaaattg taaatttaac atcttttaca ctccttcaat tatcttcatt
    26581 tgtaaaccac ttctgcgacc taggatttgc ttcaaatgtt ttacaaggac agtataacac
    26641 ggaaattggc ttattttaga aaatcgcata tttgatattt tttcttatag aaatttcaga
    26701 tttgcgattt tggtagattt gattatttcc ctggtataat aaagttatta ctaatgagga
    26761 gggaagtaat atgaagaaac aagttttaac attattgacg atcgttgctg atattattat
    26821 ctttttccca tttctaacta atctataagt tctttatatt gctgaaaacg caattcaaaa
    26881 agggatgtta attgtggatt ttctaatacc tgcagagatt gggtaaagcg ttcaatctct
    26941 ttttgattgc ttccctttgt ttgaagaaag acactcatct tctttaaaaa ttgccacgat
    27001 actttttcaa aaacatcgta cggacgcaac atgctctcca actcggcttc gaagattggg
    27061 atgtataaga aaagttttcg ctccatgagt tctgataaga tatttaagag tccttgcttc
    27121 atatacaatc gattgtgtac taactcttta aattccttgg atttttcgag taaggaggtt
    27181 gataaaaaga tcagatcttg attgctcaag aagggcatag tattgcaaaa aagatagagt
    27241 tcaaaccagg tccaagactc aatagcgtag aggtaggtag tcaaaaactc gctatcctcc
    27301 tctgttagcg ggtatctttt atctaaggaa tggagtgcat ttttaatcac aatggcattc
    27361 aaacgacgat aggtctgcgc catctgttct tgatcgactt cctccaatag ctgctctaaa
    27421 gcagctatat cctgatgggc aaagcgatcc acaacctttc gaccgattcg catatgtgga
    27481 gattcttgat agttgttgag cttgtgccca aactcatcaa aattcatatt gatcccttgg
    27541 atggctagga tcagcttgtc tgcagacagc atagactgtc ctagttcaaa cttggacaac
    27601 tgagaggctg ttagtccagc acaagccaca tctgactgct tgagctttct agccaagcgc
    27661 aattccttgt aaaattcccc cagttccatt ttttcaatca tcctaccacc tcctagcttt
    27721 tgcatattat atcattattt gtgttcattt gtcaaaacat tctgacactt tggtgattga
    27781 aaaagccagc cttaagctga ctctaaaagg accgagcctt gtacaataca gaactcaatc
    27841 ctcgttctaa atagaatgtc taactttttt gggcagtaca cgagcttggg gaacagccac
    27901 tggaacgatg aggtgtgagc tcaaaatatc ctccagttat gtttttccta atagtatacc
    27961 ggaagagtga aaggatttta taatggagca gttacaaaga acctactttc tattaaacag
    28021 tatactatga aaatgtgaaa atttaacatt tttttgtaca aattttataa attattgcct
    28081 ttttaatatc aatagttaat ctcttatcca gatccccctt
//
LOCUS       DAVLTM010000015        27992 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLTM010000015 DAVLTM010000000
VERSION     DAVLTM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.015"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..264
                     /locus_tag="VYN16_000545"
     CDS             <1..264
                     /locus_tag="VYN16_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1403120.1"
                     /translation="SQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFH
                     QFGENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            597..980
                     /locus_tag="VYN16_000546"
     CDS             597..980
                     /locus_tag="VYN16_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1403121.1"
                     /translation="MIHKIRFEHFSYDIVCPETVDNQPIPKLFLLPLVENAIKHGMQY
                     RIDLHIDIHITLEADSLTFLVKDNAGGLTKQERFAILDSLESPHTQHGIMNSYKQLSN
                     FFSDVQLDLGVNRQGETWVKFVTKD"
     gene            984..1211
                     /locus_tag="VYN16_000547"
     CDS             984..1211
                     /locus_tag="VYN16_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1403122.1"
                     /translation="MFKLIIVEDEHLIRKWLEIAVDYSALGIQVVGTASHGQEGMKLI
                     QEKETHICLNRHHNANYGCFYDVQSHSYSFL"
     gene            1260..1493
                     /locus_tag="VYN16_000548"
     CDS             1260..1493
                     /locus_tag="VYN16_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV1403123.1"
                     /translation="MQYGAVDFLSKPIDTKELTECLQKIVLRLQISTSQETPFLEEYQ
                     TLLTSIQQVDTQNQIIQQIISFVHKHYAEHYYC"
     gene            1592..1735
                     /locus_tag="VYN16_000549"
     CDS             1592..1735
                     /locus_tag="VYN16_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1403124.1"
                     /translation="MAESPNSPLSAISDQVGFSDYKYFAKVFKKHLHISPKELKLLGR
                     IVK"
     gene            1952..2548
                     /locus_tag="VYN16_000550"
                     /pseudo
     CDS             1952..2548
                     /locus_tag="VYN16_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            2705..3073
                     /locus_tag="VYN16_000551"
     CDS             2705..3073
                     /locus_tag="VYN16_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1403125.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            3086..3472
                     /locus_tag="VYN16_000552"
     CDS             3086..3472
                     /locus_tag="VYN16_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403126.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYAD"
     gene            3479..3568
                     /locus_tag="VYN16_000553"
                     /pseudo
     CDS             3479..3568
                     /locus_tag="VYN16_000553"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3554..4177
                     /locus_tag="VYN16_000554"
     CDS             3554..4177
                     /locus_tag="VYN16_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403127.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIRAIQHEVGITTVYVTHDQEEAMAI
                     SDQIAAMKDGVIQQIGRPKELYHKPANEFVATFIGCTNIIPTNLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            4167..5802
                     /locus_tag="VYN16_000555"
                     /pseudo
     CDS             4167..5802
                     /locus_tag="VYN16_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(5905..6597)
                     /locus_tag="VYN16_000556"
     CDS             complement(5905..6597)
                     /locus_tag="VYN16_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1403128.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(6841..8178)
                     /locus_tag="VYN16_000557"
     CDS             complement(6841..8178)
                     /locus_tag="VYN16_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1403129.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(8188..8454)
                     /locus_tag="VYN16_000558"
     CDS             complement(8188..8454)
                     /locus_tag="VYN16_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1403130.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(8719..9105)
                     /gene="rplQ"
                     /locus_tag="VYN16_000559"
     CDS             complement(8719..9105)
                     /gene="rplQ"
                     /locus_tag="VYN16_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1403131.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(9117..10052)
                     /locus_tag="VYN16_000560"
     CDS             complement(9117..10052)
                     /locus_tag="VYN16_000560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1403132.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(10095..10478)
                     /gene="rpsK"
                     /locus_tag="VYN16_000561"
     CDS             complement(10095..10478)
                     /gene="rpsK"
                     /locus_tag="VYN16_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1403133.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(10496..10861)
                     /gene="rpsM"
                     /locus_tag="VYN16_000562"
     CDS             complement(10496..10861)
                     /gene="rpsM"
                     /locus_tag="VYN16_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1403134.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(10879..10995)
                     /gene="rpmJ"
                     /locus_tag="VYN16_000563"
     CDS             complement(10879..10995)
                     /gene="rpmJ"
                     /locus_tag="VYN16_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1403135.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(11020..11238)
                     /gene="infA"
                     /locus_tag="VYN16_000564"
     CDS             complement(11020..11238)
                     /gene="infA"
                     /locus_tag="VYN16_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1403136.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(11355..11993)
                     /locus_tag="VYN16_000565"
     CDS             complement(11355..11993)
                     /locus_tag="VYN16_000565"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1403137.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHCVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(12144..13454)
                     /gene="secY"
                     /locus_tag="VYN16_000566"
     CDS             complement(12144..13454)
                     /gene="secY"
                     /locus_tag="VYN16_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1403138.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(13467..13907)
                     /gene="rplO"
                     /locus_tag="VYN16_000567"
     CDS             complement(13467..13907)
                     /gene="rplO"
                     /locus_tag="VYN16_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1403139.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(14052..14276)
                     /gene="rpmD"
                     /locus_tag="VYN16_000568"
     CDS             complement(14052..14276)
                     /gene="rpmD"
                     /locus_tag="VYN16_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1403140.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(14248..14742)
                     /gene="rpsE"
                     /locus_tag="VYN16_000569"
     CDS             complement(14248..14742)
                     /gene="rpsE"
                     /locus_tag="VYN16_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1403141.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(14760..15116)
                     /gene="rplR"
                     /locus_tag="VYN16_000570"
     CDS             complement(14760..15116)
                     /gene="rplR"
                     /locus_tag="VYN16_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1403142.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(15200..15736)
                     /gene="rplF"
                     /locus_tag="VYN16_000571"
     CDS             complement(15200..15736)
                     /gene="rplF"
                     /locus_tag="VYN16_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1403143.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(15928..16326)
                     /gene="rpsH"
                     /locus_tag="VYN16_000572"
     CDS             complement(15928..16326)
                     /gene="rpsH"
                     /locus_tag="VYN16_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1403144.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(16611..16880)
                     /gene="rpsN"
                     /locus_tag="VYN16_000573"
     CDS             complement(16611..16880)
                     /gene="rpsN"
                     /locus_tag="VYN16_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1403145.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(16898..17440)
                     /gene="rplE"
                     /locus_tag="VYN16_000574"
     CDS             complement(16898..17440)
                     /gene="rplE"
                     /locus_tag="VYN16_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1403146.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(17464..17769)
                     /gene="rplX"
                     /locus_tag="VYN16_000575"
     CDS             complement(17464..17769)
                     /gene="rplX"
                     /locus_tag="VYN16_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1403147.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(17847..18215)
                     /gene="rplN"
                     /locus_tag="VYN16_000576"
     CDS             complement(17847..18215)
                     /gene="rplN"
                     /locus_tag="VYN16_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1403148.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(18241..18501)
                     /gene="rpsQ"
                     /locus_tag="VYN16_000577"
     CDS             complement(18241..18501)
                     /gene="rpsQ"
                     /locus_tag="VYN16_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1403149.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(18526..18732)
                     /locus_tag="VYN16_000578"
     CDS             complement(18526..18732)
                     /locus_tag="VYN16_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1403150.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(18742..19155)
                     /gene="rplP"
                     /locus_tag="VYN16_000579"
     CDS             complement(18742..19155)
                     /gene="rplP"
                     /locus_tag="VYN16_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1403151.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(19159..19812)
                     /gene="rpsC"
                     /locus_tag="VYN16_000580"
     CDS             complement(19159..19812)
                     /gene="rpsC"
                     /locus_tag="VYN16_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1403152.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(19825..20169)
                     /gene="rplV"
                     /locus_tag="VYN16_000581"
     CDS             complement(19825..20169)
                     /gene="rplV"
                     /locus_tag="VYN16_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1403153.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(20181..20462)
                     /gene="rpsS"
                     /locus_tag="VYN16_000582"
     CDS             complement(20181..20462)
                     /gene="rpsS"
                     /locus_tag="VYN16_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1403154.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(20566..21399)
                     /gene="rplB"
                     /locus_tag="VYN16_000583"
     CDS             complement(20566..21399)
                     /gene="rplB"
                     /locus_tag="VYN16_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1403155.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(21417..21713)
                     /locus_tag="VYN16_000584"
     CDS             complement(21417..21713)
                     /locus_tag="VYN16_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1403156.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(21713..22336)
                     /gene="rplD"
                     /locus_tag="VYN16_000585"
     CDS             complement(21713..22336)
                     /gene="rplD"
                     /locus_tag="VYN16_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1403157.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(22361..22987)
                     /gene="rplC"
                     /locus_tag="VYN16_000586"
     CDS             complement(22361..22987)
                     /gene="rplC"
                     /locus_tag="VYN16_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1403158.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(23203..23511)
                     /gene="rpsJ"
                     /locus_tag="VYN16_000587"
     CDS             complement(23203..23511)
                     /gene="rpsJ"
                     /locus_tag="VYN16_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1403159.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(23777..24394)
                     /locus_tag="VYN16_000588"
     CDS             complement(23777..24394)
                     /locus_tag="VYN16_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1403160.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(24391..24981)
                     /gene="nrdG"
                     /locus_tag="VYN16_000589"
     CDS             complement(24391..24981)
                     /gene="nrdG"
                     /locus_tag="VYN16_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1403161.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(24974..25480)
                     /locus_tag="VYN16_000590"
     CDS             complement(24974..25480)
                     /locus_tag="VYN16_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403162.1"
                     /translation="MELRRPRLADKETVLEMMTEFEKFQSPHDGGFWNTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVVFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(25524..25664)
                     /locus_tag="VYN16_000591"
     CDS             complement(25524..25664)
                     /locus_tag="VYN16_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403163.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(25676..27883)
                     /gene="nrdD"
                     /locus_tag="VYN16_000592"
     CDS             complement(25676..27883)
                     /gene="nrdD"
                     /locus_tag="VYN16_000592"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1403164.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
BASE COUNT         8027 a         6140 c         5121 g         8704 t
ORIGIN      
        1 tctcaaaata aaactatcgt tttaagaatc ccagttattc ctaattttaa caatagttta
       61 gaggatgcag aaaaattcgc tactctattt aactcattaa atatcgacca agttcaacta
      121 ctcccttttc atcaatttgg tgaaaacaaa tatcgtttat taaatcggaa atatgaaatg
      181 gatggaatca acgcacttca tcctgaagat cttattgatt atcaaaaggt atttctgaac
      241 caccatatta attgttattt ctagttcatt tccttgaaat gctctagcta tttgcagata
      301 acaagcatct ataatacata cttaactttt caaaaggttt agctaaaaaa ttttagccaa
      361 accttttcta ttttaccttg ctctagaatt tttaaactgc tatacttatc acaaaaaaac
      421 cttccaatga ggtagcggat gtctcattcc tttaaaaaat ctctccaaaa agaaattctc
      481 catagaagct ctatcgcagc ctttgtgacc tcacgcgcat tctccgatac agtatcaccg
      541 atttagaaaa agaaacaacc atttgtcagg atttaaagat tatagaggat taccttatga
      601 ttcataagat tcgtttcgag cacttttcct atgatattgt ctgcccagaa actgtcgata
      661 atcagcctat ccctaaactt ttcttgcttc ctctagtaga aaatgctata aaacatggga
      721 tgcaataccg tattgatctt catattgata tccacattac cttggaagca gattctctaa
      781 cttttttagt caaggataat gctggaggac tcaccaaaca agagcgattc gctattttag
      841 actctttaga aagccctcat acccagcatg gcatcatgaa cagctacaaa caattgagca
      901 atttcttttc tgatgtccag ctagatttag gagtcaatcg ccaaggagaa acttgggtca
      961 aatttgtaac gaaagactaa ctcatgttta agctcattat tgtagaagat gaacacctca
     1021 tccgaaaatg gctagagatt gccgtagact actctgcctt aggcatccaa gtcgtaggaa
     1081 ctgccagtca cggtcaagaa ggaatgaaac tcatccaaga aaaagaaact catatttgtc
     1141 ttaacagaca tcacaatgcc aattatggat gcttttatga tgttcaaagc cactcgtact
     1201 ctttcttatg aaaaagttat cttatcaggt tataacgatt ttcaaaatgc caaaaagcca
     1261 tgcaatatgg agcagttgat ttcttatcca agccaattga taccaaggaa ttgacagaat
     1321 gtcttcaaaa aattgttttg cgattacaaa ttagcacatc tcaagaaact ccttttttag
     1381 aagaatatca aactttactc acctctatcc aacaggttga tacacaaaac caaatcattc
     1441 aacaaattat ctcctttgtc cacaagcact atgcagagca ttactattgc tgaagcttta
     1501 aactataacg aaagctatct atataaggtt atcaaagatg actctaaatg aatatatctt
     1561 acaatatcgc ctgaaacaag ctatagataa gatggctgaa tctcccaact cccctttaag
     1621 cgctatctct gaccaagttg gattttcaga ctataaatat tttgccaaag tatttaaaaa
     1681 gcatctccat atttccccaa aggaattgaa attactcgga agaatagtaa aataggctat
     1741 tcttcttttt gtagagaatt ttactctaat atagtgaatt gaaataagat gtgaacatct
     1801 ctatcaggaa agtcaaatta atttatagaa atattttagc agtcaaagtg tactgttata
     1861 gattcaatac actatagaat agaattgtag gttttaaaaa cgcttacatt gttttaaaat
     1921 aaacttaaat aacacaacgg aggtatccat catgaaaaag aaatggatgt attatgctgc
     1981 ttgttcttct aatgaatctg ccgatgacag ttcatctgat aaaggagacg gtggttcgct
     2041 agtcgtttat tgaccaaact cagaaggctt aattggagca actattcctg cctttgaaga
     2101 aaaatatggt atcaaaatag aactgattca agctggtact ggagaacttt tcaaaaaact
     2161 agagtcagaa aaagaagttc ctgtagctga tgttatcttt ggtggttctt atacacaata
     2221 tgctacccac ggagaactct ttgaaaacta tatttcaaaa gaaaatgata atgttatcaa
     2281 agaatatcaa aacacaactg gcttctctac tccttataca ctagatggta gtgttttaat
     2341 cgtccaccct gatttaacta aaggcatgaa catcgaagga tatagcgacc ttctcaaacc
     2401 tgaactaaaa ggaaaaatcg caactgctga cccagcaaac tcttctagcg cctttgctca
     2461 attaacaaat atgctacaag ctcaaggtgg ttacaaagat gataaggctt ggtcttatgt
     2521 aaaagatctt ttcacactta ttgatggtat agtaaaatga aataagaaca ggacaaatta
     2581 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
     2641 ttcaatacac tataaaatcg gtttaagttc atctggtgtc tataaagcag tcgttgatgg
     2701 agaaatggct gttggtctct cttatgagga tccagcagtt aaactcttaa atgacggagc
     2761 taacattaaa gtagtctatc caaaagaagg aaccgtcttc ctacctgcta gtgctgctat
     2821 cgttaaaaaa gctaaaaata tggaaaatgc caagaaattt atcgatttta ttatctctca
     2881 agaagtacaa gatacacttg gtacaaccac tactaaccgt cctgttcgta aaaatgctaa
     2941 aacaagcgaa aacatgaaac caattgacaa aatcaaaaca ctcactgaag attatgatta
     3001 tgtcatcaag aataaatcag atatcgttaa gaaatacaac gaagtcttta cagatatcca
     3061 atctaaacag taaaagaggt tcactatgag tgagatcgaa attattaacg ccaaaaaaat
     3121 ctaccacgat gtccctgtta ttgagaattt gaacattaca attccaaaag gaagtctctt
     3181 tacccttctt ggagcttcag gatgtgggaa aacgaccctt cttcgtatga ttgcaggttt
     3241 caacagtatc aaagatggag aattttactt cgatgataca aaaatcaata atatggaacc
     3301 cagcaaacgc aatatcgggc tggttttcca aaactacgct attttcccac atttgactgt
     3361 ccgagacaac gttgcttttg gtcttatgca aaagaaggtt ccaaaagaag aattgattca
     3421 acagaccaac aagtatcttg aactcatgca aattgctcaa tatgcggatt gaaagcccga
     3481 taaactcagt ggtggacaac aacaacgtgt caccttggca tgcgccttag cggttaatcc
     3541 aagtgttctc ctcatggacg agccacttag taatctggag gccaaacttc gcttggatat
     3601 gcgtcaagcc atccgagcaa tccaacacga agtgggaatt acaactgttt atgtaaccca
     3661 cgaccaagaa gaagccatgg ctatttcaga ccaaattgct gctatgaaag atggggtgat
     3721 ccaacaaatc ggccgaccaa aagaactcta tcataaacca gctaatgagt ttgtggcaac
     3781 ctttatcgga tgcacaaata ttatccctac taatcttgaa aaacggagcg acggcgctta
     3841 tatcgtcttt tcagatggct atgcccttcg aatgccagct cttgatcagg ttgaggagca
     3901 agctattcat gtaagcattc gtcccgaaga gtttatcaaa gatgaatctg gagatattga
     3961 aggaactatt agcgatagcg tctatcttgg actaaatacg gagtatttca ttgagacagg
     4021 ttttgcctca aaaattcaag ttagtgaaga atcaactttt gaagaagaac tacaaaaagg
     4081 caatcgtatt cgtctacgaa tcaatacgca aaaattaaac atcttttctg cagatggttc
     4141 ccaaaacctg ataaaaggag tcaaccatgg aatgtaaaaa actaaatatt tggacaacct
     4201 cctctttctt catctttctt acctatcttg tctttctcgt ttatcctatc gttaccgtgc
     4261 tcaagcaagc acttatacat gaaggacaat tctcactagc taattttgtc actttcttta
     4321 gtaaagtcta ctactctgag acactagtca acagtttcaa ggtttccatt accgctactg
     4381 tcacttcctt agttgtagga accctattag cttatctctt ctctatgtat gacttcaagg
     4441 ggaagaaatt tctacaaata ttgattatca ttgcttccat gtcagctcct ttcgtaggag
     4501 cctactcctg ggttctcttg ctaggacgaa atggggtcat cactaaattt ttgacaaatg
     4561 cccttcatct tccagctatc gatatttatg gattcaaagg aattgtactt gtctttacac
     4621 tgcaactatt cccactggta tttctatatg ttgctggaac aatgaacagt attgacaatt
     4681 ctctacttga agcagctgaa agcatggggt ccttcggatt taaacctatc gtaacggttg
     4741 ttttacctct cctagttcca accttactag cagctccttg cttgtattta tgagagcatt
     4801 ctcagacttt ggaacgccta tgttgattgg cgaaggatat cggactttcc ctgtcctgat
     4861 ttatacccaa tttattagcg aggttggagg aaattctgct tttgcaatta tggcgattat
     4921 cattgccttg gcaattttcc ttatccaaaa acacattgca aaccgctaca gtttcagcat
     4981 gaatctgctc catccaattg agcctaaaaa aactacaaaa ggaaaaatgg ctgccattta
     5041 tgcaacagtc tacggaatta tctttatctc tgttttacct caaatctact taatttatac
     5101 ctctttccta aaaacatcag gtatggtatt tgttaaaggt tattctccaa acagttacaa
     5161 ggtagctttc aatcgtatgg gatctgctat tttcaatacc attcgtatcc ctttgattgc
     5221 cttagttcta gttgttctat ttgcgacatt tatctcctac ctagccgtta gaaaacggaa
     5281 tttgtttaca aacttaattg acagcctcag tatggtacct tatattgtac caggaaccgt
     5341 tctagggatt gccttcattt ctctggtcta tttggaagtg gatttcttat gattacaggg
     5401 actgctttca tcttgattat gtctctatct gtcagaagat taccgtatac tattcgctca
     5461 tctgttgcta gcttacaaca aatagcacca agtattgaag aagctgctga aagcttagga
     5521 agtagtcgtc tcaatacctt tgctaagatt acaactccaa tgatgctatc tggtatcatt
     5581 tctggagcca tcttatcttg ggtcacaatg atttcaaaac tctctacttc tatcctcctc
     5641 tacaatgtca aaacaagaac aatgactgta gctatttata cagaggttct cagaggaaat
     5701 tacggtgtag ccgcagcctt gtcaactatc ctgactgttc taacagtagg ttccttgctc
     5761 ttgtttatga aaatctctaa aagcaatagc attacacttt agttttccac atcaaaaaca
     5821 gccgaaagga gtaccctcgg ctgttttttc ttatcttgat atccttatca aatccctagc
     5881 tcatggcaag caagtctaga ctgattaaat agagccttct tccatcttct cacgtcctag
     5941 ctctcggtaa ctacggtcac cgacaacttc aaccctaaac tgaccgtcct catattcaag
     6001 aatcgtcacg ctaccattat ctagaccatg cggatgcatg ccattaatca gataaacaat
     6061 ggttccaata gtcattccat ggctgacaac aagggcgtta cctccacctt gatcttccat
     6121 ttcttttgca attatttcaa agccttcctt gattcggcca ctgagttttt cccagccttc
     6181 agcccaacca gctgtatcga cctctaccaa gccctcagcc agttcagcat aagacaattg
     6241 gtgaacgtgg tccacattaa agatacgagg aataatgccc atgaaaagat cgccatcata
     6301 ggctccatca aaactaccga aacaccattc tctgatacgc ttgtccatgc gataagggat
     6361 tttcccctgc aagccaagct cttcaaggat aattcccatg gtctgaatgg tacgaccaga
     6421 atcactcgaa taagcgcgct caaactgtag atcagattct cgcaaaccga ttcctaactc
     6481 ttgaatccct cgttcacctt cagcagttaa gggagtatcg ctccaacctt gcgcgcgacc
     6541 aatcgtgtta aacatggtct tgccatgacg taccaaatac aatcgtactt ttaccatttt
     6601 ccgtcctccg tttgcttcta ttatatcatg aatgataaaa taaaagccac ccatacaggc
     6661 gacttttgaa ggagattatt atgaaaaagt ttaggagttc attaaataaa gatattagat
     6721 gaaaatcaaa ttcaaactaa ttcagtgtta tctgtttcaa ataatattag gaggtcttta
     6781 tttaatgatt atagtataca catctaatct taaatagaac ttaaaatttc tcctattttc
     6841 ttaattttta aaactatgaa ttggtgctgg gatttgtcca ccgcgagaga tgaagtcgac
     6901 agacgaagcc ccattaacct tcataacggg tgcagttcct aatagaccac caaactcaat
     6961 catatcgcct tctcttcctt tgggaatgat acgaacagct gttgttttca tgttgataac
     7021 accgattgct gcttcatccg caatcatagc cgcaatagtt tcagcaggcg tatcttctgg
     7081 gatggcaatc atatccaatc caacagaaca gatagccgtc atagcttcta gtttttctaa
     7141 attaagagag ccattttgca ctgcagcaat cattccttca tcctcagaaa cagggataaa
     7201 ggcaccagat agaccaccga cctggttaca ggccatcact ccaccctttt taacttggtc
     7261 gttcaagagg gccaaggcag ctgtcgttcc atgcgtgcca actgtttcta gccccatttc
     7321 ctcaaggaca cgtgccacag agtctccaac cgcaggggtt ggtgccaaac tcaagtccac
     7381 aataccaaac tccacaccca gtctctcact ggccatttga ccaaccaatt gaccgatacg
     7441 agtgatttta aaggcagttt tcttaactgt ttcggctact acatcaaagc tctgtccacg
     7501 aactttttcc aaagcacgtt tcacaacacc aggaccagaa actccgacat tgatgataac
     7561 atctgcttcc ccaacaccat gaaaggcacc cgccataaat ggattgtcct caacagcatt
     7621 agcgaataca accaacttgg ccactcccat atctgaaaga tttgctgttt ccttgataat
     7681 tcgtcccata tctgccacag ccgtcatatt aataccagac ttggttgagc cgatattgac
     7741 tgacgagcag accttatccg tctcagccaa agcgcgagga atggaattga tgagaatctc
     7801 atctcccttt tgataacctt tttgtactaa ggcagaaaaa ccaccaataa agtccacacc
     7861 aatctctttc gcagccttat caagcgcttt tgccagaacc acgtagtccg tcgcatctgt
     7921 cgctgcccca atcagagaaa taggtgtcac cgatacacgc ttattaacga taggaattcc
     7981 caactcagcc gcaatttcat caccaacagc tactaaatta gccgcctttg tcgtaatttt
     8041 ttgatagatt ttctccgcag cacgattgat atttggatcg atacagtcca aaagagaaat
     8101 ccccatggta atggttctaa tatcgaagtt ttgctcctca atcatggcga tggtttcagt
     8161 aacttgtcta atatccatga tgacctccta gatattatac atagcttcga aaatcgctgc
     8221 actctgaata ttgattttta cattcaaagt ttgcccaaaa gcttcaaatt cattacgaag
     8281 ataggtaaaa tcttgctttt catcactaga tacaacagcc atcatcgtaa aatattcatc
     8341 caatacagtt tgagagatat cgtcaatatt caatcctaat tctgcaattt taccagaaac
     8401 acctgcaaca attccagatt tatctttacc aacaacagtt ataatcgctt tcataagtaa
     8461 actccaattc ttcttaaaat aagaaacctt aacattaaac aggattcttt tcaaatagta
     8521 tagcataatt ctaacgaaaa tactcaaaaa cgcctgctta cgaagttgtt cttaagaaaa
     8581 aaacaatttc gtaaacaagc gtctactatc ccaacttatg atgagtgttc ccgctaggac
     8641 cgaaatccta gcggtagacc agagctagac taagagcaca agactccatc atcataacac
     8701 tcaacaaaat tgatgatttt atactaattc gatgatcgcc attggcgctg catcaccacg
     8761 acgtggttca gttttaagga tacgagtgta tccaccgtta cgttcagcat aacgaggtgc
     8821 gatttctgag aacaattttt gaagtgctgt agtagaagtg tacttatcag ttgcttcatc
     8881 atagttttca gatgcaattt cattacgtac gaaagcagct gcttgacgac gtgcatgcaa
     8941 atcaccacgt ttacctagag taatcatttt ttcaacagtt ttacggattt ctttagcacg
     9001 agcttcagtt gtcacgattg attcgttgat caaaaggtca gttgtcaaat cgcgaagcat
     9061 tgctttacgt tgtgagctag tgcgtcctag tttacggtaa gccatgtatt cctcctttat
     9121 ttatctttta atccaagacc caaatcaatg agtttgagtt tcacttcttc caaactcttg
     9181 cgtccaagat ttcgtacttt catcatctct gcttcagatt tttctgtcaa atcatgcaca
     9241 gtattgatac cggcacgttt taaacagttg tatgaacgca cagacaagtc cagttcctca
     9301 atcgtacgat ctaaaatacg gtcgtcagat tcagtatcag cttctttcat cacttcagtt
     9361 gacttagcaa tctcagtaag atttgtaaac aaatcaagat gttctgtcaa aatacgtgct
     9421 gaaagcccta aagcatcttc tggaataatt gttccatttg tcaagatttc aagggttaat
     9481 ttgtcgaaac catcattgct acctacacga gcaggttcca cttgatagtt gacttttgta
     9541 actggtgtat aaatagaatc tacagcaagt gttccaactg gtgcattatc ctttttattt
     9601 tcatcagcag gtacatatcc acgaccactg ttaacagtca tagtcgcttt tagagaagaa
     9661 ccttcaccga ttgtaaagag ataatgatct ggatttacaa tttcaatatc gctatctgtc
     9721 aaaatgtcac cagctgttac ttcagcagga ccttcaacat ccagttcgat gattttttcg
     9781 tcttcaacgt acgatttcac tgcaattcct ttaatgttca gaatgatttg catcacgtct
     9841 tcacgaacac ctggaactgt gtcaaactca tgtaacacac catcaatgtt gatagatgtc
     9901 acagctgctc ctggtagaga agctagaagt acacgacgaa gagagttacc aagagttgta
     9961 ccgtagccac gttcaagtgg ttcgattaca aacttgccat aatctttatt ttcatcaatt
    10021 tttgttatat ttggtttttc aaactcgatc atttagttac tccctcttaa acgaaaagca
    10081 gtgtaatgcg atgattatac acggcgacgt tttggaggac gagcaccatt gtgtggcact
    10141 ggagtcacat cacgaattgc tgttacttca agaccagcgg cagcaagcgc acgaatagct
    10201 gactcacgac cagaacctgg accttttaca gtaacttcaa ctgatttaag accgtgttct
    10261 tgtgcagatt tagcagcagc ttcagaagcc atttgagcag cgaatggtgt agatttacga
    10321 gaacctttga aaccaagagc accagctgat gaccaagcaa ttgcattacc atgcacatca
    10381 gtaatcataa caatagtgtt attaaatgta gcgtgaatat gagcaatacc agattcgata
    10441 ttctttttca cacgacgttt acgtgttggt ttagccaaga cttttacctc ctatattatt
    10501 ttttcttacc agcaatcgca acagctttac ctttacgagt gcgagcgttg tttttagtat
    10561 tttgtccacg gacaggaagt ccacgacggt gacggatacc acggtatgaa ccgatttcca
    10621 tcaaacgttt gatgttcaag tttacttcac gacgaaggtc accttcaact ttgattgcat
    10681 ccacttcacg acggatagca tcttcttgat ctgatgtaag atcacgtaca cgaacatctt
    10741 ctgagattcc agcagcagcc aaaattttct tagatgttgc aagtccgata ccataaacat
    10801 aagtcaatga gattactacg cgtttgtcat ttggaatatc aactccagca atacgagcca
    10861 tgttttctcc tttctatctt atccttgacg ttgtttgtgt tttggatttg ctgggcaaat
    10921 taccataaca cgaccattac gacgaataac tttacagtat tcgcaaattg gtttgaccga
    10981 tggtcttact ttcatttctt atccctccaa gtttttcgat tatttaaagc ggtaagtgat
    11041 acgtccacgt gtcaagtcat atggactcat ttcgacagta acacgatctc ccgctaaaat
    11101 acgaatatag tttttacgaa ttttaccaga aactgttgct aaaatctgat gtccattttc
    11161 aagttcaacc gtaaacattg cattcggcat tgtatcaact actttgcctt caacttcaat
    11221 cacatcgtct tttgccacgc aaaagcacct ccataaattt cgattcgatg cctctagaca
    11281 cagagacaac aattataagt cagactatct cagtataaca tttgtagcgg atttttgcaa
    11341 gtgtgaaaaa cgctttattt caaatttgtc aatacttttt caatatctga gaagacatca
    11401 ttgatatctt gattaccttc gatgtcatga accaaacctt tggcacggta gtgagcaatg
    11461 attggttctc cttgagcaat attaacatcc aaacgacgtt ttactgtctc aggcttatca
    11521 tcttcacgtt ggtagtaatc ttcttcttta tagtcaactg gtgggttaaa gaccttgtgg
    11581 aaagtttctc cagttacgca gtggatgata cggccactca aacgttccaa gaggctgtca
    11641 gggttcactt caatattgat aataccttct agttcaatgc caagttcagc caatgttttg
    11701 tccaaggcat gagcttgttc aattgtacgt gggtaaccat ccaataagaa tcctgtttct
    11761 ttaatatcat cttgtgaaag gcgttctttt acgattccat ttgtaacttc gtcaggaacc
    11821 aattcaccct tgtcaatata tgacttagca agaacaccca tttcagtttg atttgccatt
    11881 gcagcgcgga acatatcacc tgttgagata tgtgcaacat ggaattgttc tacgattttt
    11941 gctgcttgag ttcccttacc tgcaccaggt aagcccataa tcaaaagatt catgatttga
    12001 tctccttatt ttatttttaa atagaagcaa aaagtctttt cacccctatt taaaaacaaa
    12061 atagaagagg ggagatccaa ctcttacaaa tgtcagagtt taaacctcca ctccctcagt
    12121 atttactgat tcagtaaata cttttattct gttctgtcca tgaaaccaac atacttacgt
    12181 ttcaataggt aaccttccaa ttgcttgatt ccttcgatac ctgtagagat aatgatcaag
    12241 agacttgttc caccaaaggc aacaacatca gaaagaccaa atacatcttt agctgcaatc
    12301 ggtaaaatgg aaatcacacc aaggaagagg gaaccaacag ttgcaagacg acgaagaagt
    12361 ttagacatat attcttctgt acctttacca ggacgaactc catggatata ggcaccactc
    12421 ttttgtaggc tctctgctgc tttttcagga ttaatctgta caaacgtata gaagaatgta
    12481 aagagaataa tcaacaaagc atacatggca ataccagttg gagaagtagt tgccaacatc
    12541 tcttgtgcta cccttaccca agcccaatca tgacctgtgg cactcaaaaa ctgaagaata
    12601 gccgcaggcg ctgcagtaat cgaactggca aagataacag ggataactcc agcaggattt
    12661 acctttaacg gaaggtaaga gctagatgga gcaccttgtg caaccttagt atattggatt
    12721 ggaattttgt attctgcttg ttgaacataa gttgtaaagt aaataatcaa caatacagta
    12781 ataatcaaaa tgattacgaa aatgatagat gaagtgatac ggctacttgg gacgttcaca
    12841 aagtagtcca catagatgcc ctgaatcatc tctggaattg aagaaacaat cccggcaaag
    12901 ataatcatgg aaacaccgtt tccgtatccc ttatctgtaa tttgctcacc caaccaagtg
    12961 acaatcatac taccagctgt taagatgata ccaatcgtca gaaaaacttg tggagttaaa
    13021 gcagttttaa tcaattgagc tccagccaag gtattaaaac cagctgtaat cccgatagat
    13081 tgcacaaaag cgagaactag agcaatataa cgagtagctt gattcaattt tcttcgacct
    13141 acttcccctt gtttacccca ctctacaaac ttgggtaaaa tatccatttg caagagttgg
    13201 acaacaatag aagcggtgat atagggacta actcctaagg caaaaatcga aaagtttttt
    13261 agggcattcc ccgacaccaa gctcaacatg tttaagaagg ataatccact taaagcattc
    13321 aagctattgg cattcacacc aggaactgta atgctagttc cgatacgaaa gaccaaaacg
    13381 ataaaaattg taaataaaat ttttgatcga acctgcttga ctttaagagc ttctcttaat
    13441 aatttaaaaa acataggtca cctctcttag atgacttcta ctgaaccacc tttagcagtg
    13501 atagcttctt cagctgattt agagaattta gctgctttca cagtcaattt cttagtcaac
    13561 tcaccgttac caagaatttt aatacctgac ttttcagctt taacaattcc tgcttcgata
    13621 agaacaactg gagttacttc agcaccatct tcaaagacgt tcaattggtc aaggttcaca
    13681 attgcgtatt ctttagcgtt gatgttagtg aatccacgtt ttggaagacg acggaacaat
    13741 ggagtttgtc caccttcaaa accaaggcga actccgccac cgctacgagc tttttgacct
    13801 ttttgaccac gaccagatgt tttaccgtta cctgatgaag taccacgacc aacgcggtta
    13861 cgtactttac gagaaccttc tgcaggtttc aattcatgaa gtttcatttt tattttctcc
    13921 tcttttgtaa aatgctagcg ccgataaggg agaaaaggtt gtctccccca tcaactcgcc
    13981 tatacgatgt catcatagat gactatatct agttttaggg gatggtacag tgcacatccc
    14041 ctaaaacttc attagtttac ttcttcaact gttactaagt gagatactgc tgtgatcata
    14101 ccacggatag cagcgttatc ttctttaata acagagctgt tcaatttgcc aagtccaagt
    14161 gctacaacag ttttacgttg tgatggaatg cgtccgattg gagacttagt caaagtaatt
    14221 ttaatttgag ccattttatc ccctttctta tgccaaatca gaaactgaaa taccacgaag
    14281 ggcagcaatt tcttcagcgc gtttcaattg tttcaaacct tcaacagttg cacgaacaat
    14341 gttgattgga gtgttagaac caagtgattt agatgtaata tctgccacac ctgccaattc
    14401 cacaacggca cgaactgcac caccagcggc aactccagaa ccttctacag caggtttcaa
    14461 caatacttta gctccaccga attctgaaag aacttcgtgt gggattgttg ttccaaccat
    14521 aggaacttcg atcaagtttt tcttagcatc atctactgct ttacggattg cttctggaac
    14581 ttcttgagct ttaccagtac caaatcctac gcgaccattg tggtcaccaa caacaacaag
    14641 agctgcgaaa cgaagacgac gtccaccttt aacaactttt gtaacacggt tgacagcaac
    14701 tacgcgttct tctaattcaa ctgcattgtc tttaaatgcc attttctagt gtcctcctat
    14761 tagaatttca atccgttttc acgagctgca tcagccaaag ctttcacacg tccgtgatat
    14821 agatatccac cgcggtcgaa caccacttct gaaatacctt tagcgtttgc acgttctgca
    14881 acgagtttac cgacagcaac ggcttgttca gttttagttc cttttgaaac ttctttatca
    14941 agagttgaag cacttgcgag cgttacaccc gctacgtcat caatcacttg agcgtagatg
    15001 cctgtattag aacggaatac gttcaaacgt gggcgatcag cagttccaga gagttttccg
    15061 cgaacgcgac ggtggcgttt ttggcggagt ttgtttttat ctggttttga aatcacagtt
    15121 ttcacctctt tagttttaaa tcgtgtgcta tgcacaaagt tggaaaatag gttggtggtt
    15181 gatgatcaac cactcaacat tatttacctg ttttaccttc tttacggcga acgaattcac
    15241 caacgtaacg gataccttta cctttatatg gttctggtga acgaaggcta cgtacgtaag
    15301 cagctgtttg accaactact tcttttgaaa ttccgctaac aacgattgtt gttgggtttg
    15361 gaagttcaaa agtaattcct tctggagctt caacttcgtc tggatgagat ttaccaacag
    15421 ccaaaacaag tttagatcct tgaagctgtg cacggtaacc aaccccacgc atttcaagtt
    15481 ctttcttgaa tccttctgat acaccaacaa ccatgttgtt caaaagggca cgagtagttc
    15541 cgtggatagt tttcatttct tttgaatcgt ttggacggtg aagagttatt tcagtacctt
    15601 ccacacggat ttcaatatct tttgagaact cacgagtaag ttctccttta ggtcctttta
    15661 cagttacaac gttgtcattg ttagcgagtt caacaccagc aggcaacacg ataactttat
    15721 taccaatacg tgacatgttt attttctcct gttaaattgt caggccagaa cggccagttt
    15781 tcacggggtt cagatactta tttagttcaa gagctgaact gaacacgctc taagaactgt
    15841 gcaaaataga taagttggct tgtttgtaaa caaacttctc caacttccta attttgcttt
    15901 gttctttacg agctttgtat cttgatttta ccaaacgtaa gcgataacct caccaccaac
    15961 attcttttgg cgtgcttctt tatcagtaag caaaccttca gaagttgaaa ggatggcaat
    16021 tccaagtccg ttaagaactt ttggaaggtc ttcacgtttt ttgtagacac gaagtcctgg
    16081 tttagaaaca cgtttcaagt tagtgataac tttctcacca tttggtccgt atttaagaaa
    16141 tacacggatg acgccttgtt tgtcatcttc aatgatttca acgtttttta caaaaccttc
    16201 gcgtttaagg atttcagcaa tccctttttt gatgtttgat gcaggtactt caagtacttc
    16261 gtgtttagct tggttagcat tacgaatacg agttaggaag tctgcgattg ggtcagtcat
    16321 aaccattttg tttttctcct cttactagta gtttgcaagt tgcacttgct agttaataca
    16381 tgatacaaag agcgtaacag tacgagcaaa aatagacaat ttaatgcaga agcaatgctc
    16441 caaagcaaat tggtcttttt tgccaaagct gtagctcgtg ttcaaattgg caagcccaat
    16501 tgaacccggg ctaaactctg tgtgaaaaag ataaactttc ctagaaactt tagtttcttc
    16561 gtcaagtttc ctatttttac ttggagtttt gacgctcttg atatcttaaa ttaccaagat
    16621 gcttttgtta caccaggaat ttgaccttta tgcgcaagtt cgcgaaaagc gatacgactc
    16681 agaccaaatt tgcggtaaac tgaatgtggg cgccccgtaa ctctacaacg attatgtaaa
    16741 cgagtcggtg aggcgttgcg aggtaattta gataaacctt cgtagtcccc tgccgccttt
    16801 aatgcagcac gtttttcagc ataacggtca acaatttttt ggcgtttagc ctctctagct
    16861 accattgatt ttttagccat tagatttacc tcctatatta ttttgcaaaa ggcattccaa
    16921 ggcctgtaag caatgcacgt gactcttcgt cagtgttagc agttgttacg ataacgatgt
    16981 caagaccacg agttttgtca acgtcatcga agttgatttc tgggaagatt aattgttctt
    17041 tcacaccaag tgtgtagttc ccgcgtccat caaatgattt tgttggaaca ccgtggaagt
    17101 cacgtacacg tggaagtgaa actgatacca atttatccaa gaattcgtac atacgttcac
    17161 cacgaagggt aacttttgca ccgatcgcaa caccttcacg aagacggaag ccggcgattg
    17221 attttttagc tttagtgata agtggttttt gacctgagat aagtgccaat tcttcagcag
    17281 ctttttcaag gcttttagcg tttgatacag cttcaccaac acccatgttc aaaacaatct
    17341 tatctacttt aggcacagcc atcactgatg agtagttgaa ttgttctgtc aaagcaggaa
    17401 ctacttcatt aagatatttt tcttttaaac gatttgccat tatacttctc ctttccttcg
    17461 tgattaatca agcacttcgc ctgatttttt gttgtagcga acttttttac cgtctacaaa
    17521 tttgtaacca acacgaccag ctacaccatt tttgtccaaa acttgaacat ttgatacgtg
    17581 gatagctgct tctttctcga tgataccacc ttgaggaagc tcgttagttg gacgttggtg
    17641 tttcttaaca atgttaacac cttcaacgat aactttgttt acttttggaa gggcagtaag
    17701 gacaacagct tctgttccct tatctttacc agcgattacg cgaactttgt cgcctttttt
    17761 tacaaacatt aggtttctcc ttgatttttc ttacgcccat aagggcaccc tagcttgaag
    17821 ctaggggact agtttgttcc taaaaattaa agtacttctg gagcaagtga cacgatcttc
    17881 atgaagccac cttcacgcaa ttcacgtgca actgggccaa agatacgtgt tccgcgagga
    17941 gttttgtctt cacggatgat aactgctgcg ttttcgtcaa atttgatgta tgaaccatca
    18001 gcacgacgag cacctgattt agtacgaacg ataactgctt taacaacgtc acctttttta
    18061 accgcaccac caggagtagc ttgttttaca gatgccacga taacatcacc gatgtttgca
    18121 aatttacgtc ctgaaccacc aagaactttg atagtcaaga tttcgcgagc accgctgttg
    18181 tctgcgactt tcaaacgagt ttctgtttga atcatttcag ttttctcctt tcaggtttga
    18241 ttagatgatg accgcttctt caacaacttc tacaagacgg aaacgttttg tagctgaaag
    18301 cgggcgagtt tccatgatac gtacgatatc gccttctttg gcaacattgt tttcatcatg
    18361 agctttgtat tttttagagt agttaatacg tttaccatag actgggtggt tacgttttgt
    18421 ttcaactaca actgtgattg tcttgtccat tttgtcagat acaacacgtc caacaagaac
    18481 tttacgatta ttgcgttcca ttgaaatttc tccttcccta gtctattatt tcgcttcaga
    18541 ttgaactgtt ttgatacgag cgatttgttt tttaacttct ttcaagcgag ctgtttgttc
    18601 caattgacca gtagcagctt ggaaacgaag ttcaaacaat tcttttttca attcgttttc
    18661 gcgcttcgcg agttcttctt gagaaagacc acgaagttct ttaacaaatt cttttacttc
    18721 attaagtttc atgccttctc cttattctgc ttcacgtttt acgaatttac atttaactgg
    18781 caatttgtgg ctagcaagtc gaagcgcttc acgtgcaatc tcttcagata caccagcgat
    18841 ttcgaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg cccctttacc
    18901 agatcccata cgcacaccga tagctttagc agtgtatgat ttgtgtggga agattttaat
    18961 ccaaacttta ccaccacgtt tcatgtaacg agtcatggcg atacgagcag cttcgatttg
    19021 gcggttagtg atccagtggc tagttgtagc ttgaagaccg tattcaccga atgctacttc
    19081 ttttccacct tttgcttcac cgcgcatttt tccacggaac tcacgacggt gtttaacacg
    19141 tttaggtact aacattggtt atttacctcc tttagtgttt ttacgagctg gaagaacttc
    19201 accacggtag atccatactt taacaccaag tttaccgtat gtagtatctg cttcttccca
    19261 agcgtaatcg atatctgcac gaagtgtgtg aagcggaaca gttccttcag agtatccttc
    19321 agcacgggcg atatctgcac cgttcaaacg acctgatact tgagttttga ttcctttagc
    19381 tccagcacgc attgcacgtt ggattgcttg tttttgtgca cgacggaaag caacacgttg
    19441 ctccaattga cgagcaattc cttcacctac aaggtgagca tccaaatcag gttgtttgat
    19501 ttcgatgatg ttgatgtgta cttgttttcc agtcaatttg ttaagttttg cacggagtgc
    19561 atcaacgtta gcaccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac
    19621 gttaactttg tttactgcgc gttcgatttc aatagttgaa actgctgcgt cagcaagttc
    19681 tttttgaacg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc
    19741 agcataccat ttggcatccc aatcacggat gatgccgaca cgcataccaa ttggatgtac
    19801 tttttgaccc acgattttac ctccttattt ttctgcaaca gctacagtga tgtgagctgt
    19861 acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcatagttgg
    19921 tccttcgttt gcgaatgctt cagatactac caagttagct ttatccaaac caaagttgtt
    19981 ttcagcgtta gctacagctg agttcaaaac tttcaagatg atttcagcag ctttgtttgg
    20041 agtgaatgtc aagattgcga ttgcatcggc tacgctttta ccacggatgt tatcaagaac
    20101 aagacgtgat ttacgaggtg aaacacgtac tgtacgagcc attgctttag ctgaagtaat
    20161 ttctgccatt tatgttctcc ttattttcta cgtgttttct tgtcgtctgc agcgtgacct
    20221 ttgtaagtac gagttggtgc aaattcacca agtttgtggc ctaccatgtc ttcttggatg
    20281 taaacaggta cgtgtttacg tccgtcataa actgcaatag tgtaaccaat gaaacttggg
    20341 aagatcgttg aacgacgtga ccaagtttta ataacttttt tcttttcgtc gttagcttga
    20401 gcttcaactt ttttcatcaa atgctcatcg acgaaaggtc cttttttaag actgcgtccc
    20461 atttttatat tttctccttt aaatgttgta ccacagcggc ttgcactcct atggagcgct
    20521 accgagctgg cggatttact agttgcttaa gcgactagtt taatattatt tctcgttgcg
    20581 acgacgaacg ataagtttgt cagatttcgc tttcttgtta cgagttttaa gaccaagagc
    20641 aggtttgccc catggagtag atggtgcttt acgaccaact ggtgctttac cttcaccacc
    20701 accgtgtggg tgatcgttag ggttcattac agaaccacga actgttgggc ggataccttt
    20761 ccaacggcta cgtcctgctt taccaaggtt tacaagtcca tgttgttcgt ttccgacaac
    20821 accaactgta gcacggcaag ttccaagaat catacgaact tcacctgatt gaagacgaac
    20881 aagaacgtat ttaccttcag aacccaatac ttgagcagat gcaccagcag cacgtaccaa
    20941 ttcaccacca cgacctggtt tcaactcgat gttgtgaatc aaagtaccaa ctgggatgtt
    21001 agcaagtgga agagcgtttc cgactttgat atctgcttct ggacctgaaa cgatacgttg
    21061 acctacttca agaccttttg gagcgatgat gtatgctttc acaccatcag tgtagtgtac
    21121 aagagcgatg tttgcagaac ggtttggatc gtactcgatt gttttaacaa ctgcttcaac
    21181 gttgtcttta ttacgtttga agtcaaccaa acggtagaaa cgtttgtgtc caccaccttg
    21241 gtgacgaact gtgatacgac cgttgttgtt acgaccagcc ttgctcttca atgcaacaag
    21301 caatgatttt tcaggagtgc ttgttgtgat ttcagcgaaa tccaaagaag tcatattacg
    21361 gcgaccgttt gttgttggtt tataaacacg aattcccacg atatttcctc cttagattat
    21421 tcagcttcag cagcaaacaa ctcgattgct ttagaatcag ctgtaagtgt gatgatagct
    21481 tttttagttt tgttagtaaa accagtgtaa cgtccaacac gtttagcttt tggttttacg
    21541 ttgattgtgt taacattggc aactttaaca ccttcgaaag cagcttcaac agcttgcttg
    21601 atcaaaagtt tgtgtgcacg agtgtcaact tcaaatacat atttccctgc ttcaagttga
    21661 gccattgagc tttcagtgat gacaggtttt ttgataacat catacaaatt cattatgcaa
    21721 gaacctcctc gattttagag atagctgctt gtgtgacaag aagtttgtcg ctatttgcga
    21781 tgtcaagaac acttgcagtt gtagcagttg caactttcac gtttggaagg ttacgagctg
    21841 aaagagctgc gaattcattt ccttcttcaa ggataacaag aactttagaa tcgatgctca
    21901 atgctgcaag aacttttgca aattcagcag tttttggagc tgtaaatgaa agagcgtcta
    21961 cagctacgaa tttgttttca gcaacttttt cagagtaaac tgatttaaga gctaggcgac
    22021 gaactttttg tggaagtttg tagccgtatg aacgtggagt tggtccgaag acaacaccac
    22081 caccacgcca ttgtggtgag cggatagaac cttgacgagc acgtccagtt cctttttgac
    22141 gccatggttt gcgtccacca cctgatactg cagagcggtt tttaacagcg tgtgttcctt
    22201 gacgaaggct tgcgcgttgg ctgatgatta catcaaacac aactgattca tttggttcga
    22261 taccaaatac tgcatcgcta agaacaactt ggccagcttc tttaccagtt tggtcaaata
    22321 atgttacgtt tgccattgtg actgatttcc cctttcttta ttatttacca gctttaactg
    22381 ctgatttgat agtgataaga gatttcttag cacctggtac gttacctttg ataaggataa
    22441 cgttcttttc tggaacaact tgtacaactt caaggttttg aattgttacg cggtcgccac
    22501 ccatacgtcc tgcaaggttt ttacctttga atacgcggtt aggtgcaaca ggtcccatag
    22561 aacctggacg acggtggtaa cgagaaccgt gagccattgg tccacgtgat tgtccgtggc
    22621 gtttgataac accttggaaa cctttacctt tagaagtacc agttacgtca acaacgtccc
    22681 cagctgcgaa tgtttcaact gtaatttcag caccaacttc caagccttca acgtttttga
    22741 attcacgaat gaagcgctta ggagccgtgt tagctttcgc tacatgtcct ttagcaggtt
    22801 tgttgctcaa tacttcgcgt ttgtcatcga aaccaacttg gatagcgttg tatccgtctg
    22861 tttcaacagt tttaacttga agaacaacgt ttggagttgc ttcaataact gttacaggga
    22921 tcaattcgcc agcttcagtg aagatttgag tcattcccac ttttttccct aagattcctt
    22981 ttgtcatgag aaaatagttc cttttctata tttttattca aaaagttttt aacgagcgtt
    23041 ttttatgctc aagtctaaga tacaaagggc gtcaaactca acgtgaaaat aggaaactga
    23101 cacagtgtct agcagacgcg aggaagttta tctttttcac accaagttta gcccgtgttc
    23161 aattagattg aaacaccagc ctctgctctc ttatatttta gattaaagtt tgatttctac
    23221 gtttacacca cttggaagat ccaatttcat caaggcatca actgtttttt gagttgggtt
    23281 aacgatatcg atcaaacgtt tgtgtgtacg catttcaaat tgttcgcgag agtctttgta
    23341 tttgtgagtc gcacgaatga ttgtgtagag gctacgttca gttggaagtg ggattggacc
    23401 cgcaacttgt gcacctgtac gagtagctga ttctacgatt tttgcagccg ctgtgtcaag
    23461 cgtacggtgt tcgtaagctt tcaaacggat acggattttt ttgtttgcca tctttttctc
    23521 cttttcgtct atttaagata ataggctagc tccacaagaa aaccgacgcg gtgttgcgtg
    23581 gcaatgcaac cgagcgtgtc gcaacctctt gcatcaaaac taaggctgta atttacagca
    23641 ccataataga ataacacaaa tcccctgcga ttgcaaggga tttgttaggt tttttcgttt
    23701 ttttgggatg aaactgtttt cctacttttt ctttctaaaa ttagggattt tcttctatat
    23761 ttcttctata cataagttaa gtcctcttga catcaaattt atcttctgat tggtccacta
    23821 agatatccgc cttagactca gtttctttat agtatcgcag atactgctcc cgtctcatct
    23881 gatggctagc caatataaag gatgcatcgc gatttctcac agtcgtatct ctagcaaggc
    23941 gtcgctttaa ttcggtctcc tcatccgtgt agaaacagat ggttttttca aagagttcct
    24001 tgggtagaaa gccaacagac atcccttcga caatcaaaat tggtttggct ccagacaaga
    24061 cctcactagc cttccaaggt tcttcaattg tcaagacatc catacccgcc tgcaaggcaa
    24121 ggatatctct ctgcaaactc tccagttcat gcgccactgg cagactggct gtcacctttt
    24181 gattcggcgc gtccttgggt actaccaaat agcgtccgga ggtgatatag ggatctgttt
    24241 ctagcaaatt tactgtagta gaatctaaag cttggtacaa ttcctgtgca aaggttgatt
    24301 tacctgaagc tccatgaccg tatattccca gtgtcctgac tttccccgtc tcgatctctg
    24361 agactagttg gtctactaag tcttttttct tcattctctc ttcacctgtt catagctttc
    24421 ttttccgtca ttgagcttgt cccaaatcac tacttgccca cttttgagcg atttttgcac
    24481 atcgataatt cgttggttgg acgaacctcg aaactggagc atgagatttc tcttagttcg
    24541 atcgtatcgt ccatcgacaa gaatgtcaat caatgataag agttccagtt tatctggagt
    24601 ttccaacatc atttcttccc aagtgtagcc cgtccaggac caaatgtcct tgtctggcaa
    24661 ttcctttcgg atgcgtttaa ctagaggcaa gagaatgcca gtattgagaa aaggctcccc
    24721 tcccagcaaa gtcaagcctt gaacataggg ttgggcaagg tctgccataa tctgctcttc
    24781 taattctgct gtatagggaa tgccagcatt aaaagaccaa gtcgcaacat tataacatcc
    24841 ctcgcagtga aacatacagc ctgatacata gagagagttg cgcacgcctt cgccgtccac
    24901 aaagttaaag gccttgtagt caatgatacg accttgacta agttcctcgc ttttccattc
    24961 ttgtggtttt ggattattca ttcgctacct ctatccaata acgctcgact ccattgcgag
    25021 catcctcaaa tattccacca tttgctagaa tgactgctct gctagcagga ttattcacgc
    25081 tacaggtcac cagagctttc ttgatgttct tttccttagc aacttgcaag ccctgacgga
    25141 gagtctcttt tgcataaccc ttgcctcttt cagatggacg aatggagtag ccaatgtggc
    25201 caccttcttc tagtagaaag ttactgaggc gcaaccggag attaagaaat ccaactgctt
    25261 gacctttctc agaaaaaacc actaactgaa ttgcaggaac ccatccttca ggcagattaa
    25321 tccccatttc ctgttcctga ttgctttcta accagtcttc atacacaaag ttctctgtat
    25381 tccagaaacc gccgtcgtga ggcgactgaa atttttcaaa ctctgtcatc atctctaaaa
    25441 ctgtttcttt atctgctaat cttggtctgc gtaattccat ccctacctcc cactatgaga
    25501 aaagcgagag ctggctctca cttttatttt ttcttgttct tgtttaaaaa ttgttctctg
    25561 aagctaagca agcgttcttt cttaccagct ccacctttag agtgtttctc gtgataacgg
    25621 gtcacttgtg cgcgcccctt atcgtctagt tgatattttc ccatttcatt tctttctaat
    25681 ttgttacttg atgcccagct attttaatcg ttgaaccatt catatgtttg acacgcgcag
    25741 cgatttcctt gtgacgcccg ttaaccattg gtctcgcttg aggattgcca agatagccac
    25801 aagtccgttt aacaacgtct actgttttag gatcgctatt gccacagttt ggacaagcaa
    25861 accctctctc agttggttca aaatcccctt caaagtcaca cttgtagcaa cggtcaatcg
    25921 gagtattggt gcctagatag cctacacggt cataagcata atcccagaca gcttccaagg
    25981 cctttggatt ttgctgaagg actggatact cacaataatg gatgaaacca cctgacgcac
    26041 ctgcttctgg atagactttc tcaaagtcca atttttcaaa cggtgttgga tttttacgaa
    26101 catcgtagtg gaaagagttg gtgtagtatt ccttgtctgt gatatcagga atagagccaa
    26161 acttgtctat atctagtcgg cagaaacggt ctgtcagact ttcggatggt gttgagtaga
    26221 tagagaaatg gtagccatat tggtctgacc actcttctac acggcgtttc atatcgtgaa
    26281 tgatgtctag cgtgaattcc ttagcatctg gattactttc ccagctgtta ccaaagaaaa
    26341 ctgtcgctac ttcatacaag ccgatatagc ccagcgaaac ggtcgcacga cgattcttaa
    26401 agagctggtc aacactttct tctttaccta gacgatggcc aaaagcaccg tactgataaa
    26461 gaataggagc attcgctggt gtcgcctctt tagtgcgttc gacacggtaa acaagagcat
    26521 cttctgcgat attcattcgc tcgttgaaga tttcccagaa cttattcata tcaccttcag
    26581 actcaagagc aatacgaggc agattaaccg tcacaacacc cagattcatg cgacctgaat
    26641 tgacttctac accattttca tccttccacc cttgaaggaa agaacggcag cccataggca
    26701 ccttgaaaga acctgtcaaa tcaacaatct tatcataaga caagacgtct ggatacatcc
    26761 gcttggttgc acactctaga gccaactgct tgatgtcata gttgggagtt ccttcctcta
    26821 agttgaggcc tcttttaagc gtaaagataa gtttaggaaa gatagccgta cggtgttctg
    26881 aaccaagacc cttgatgcga atgtttaaaa tagctttttg aatttctcgt tcaaaacgac
    26941 tggttcccag accaaaacct aacgaagtaa aaggtgtttg tccatttgaa gtgaagagag
    27001 tattgatttc atactcaaga gattgcatgg catcgtagat gtccttttgc gctttcttcc
    27061 aagcgtaatc ttcctgtttt tcaggcaata cccactcttc tgcatctttg agatgttttt
    27121 gataattctt ctctgcataa ggcgccaaaa tttcatcgat acggtcagct gaacagccac
    27181 cgtactggct agaagcaacg ttggcaatga tttgagaaat ctgtgctgtc gcagtctgga
    27241 tagacttggg actctctacc tctgcatttc caatcttaaa accattttcc aacataccct
    27301 taaaatcaat caaacagcag ttggtcatag gggtataggg actgtagtcc aaatcgtgat
    27361 agtggatatc ccccttttgg tgggcattgg ctacgtgctt aggaagcatt tgcagtccga
    27421 ttgatttccc aacaatccct gctgtcaaat cacgctgagt gttaaagaca tcactgtctt
    27481 tattagcgtt ttcattgaca actgtctggt ctttgttgag aagtttatga atactaaagt
    27541 tgatatccgt cgcttttgag cgctcaaaat ccctctgtgt ccgataagta atatactcct
    27601 cagccagcgc atattctttg gcttcaagga gttcatgttc tacgatattt tgaatttcgt
    27661 aaatcttaat tccctgtgga aagcgactat gaatttctgt aataattcgc tcagtcagat
    27721 cattgaggcg tttttcaacc aggggtgtca catccataac cttgtcagcc gccttgtgga
    27781 gagccttgtc aatcttgtcc acatcaaata caacacgtct cccatctcgt ttctcgacga
    27841 agagagttgg caaaattgta attttttctt ctagtgcaat catatgcatg ctcttttcta
    27901 aaatgttctt tctaaaaagt attataccac tctaaaaaga aaaatcaata tcttgtgtta
    27961 aaaatcctaa aatttttaaa aatacacaag at
//
LOCUS       DAVLTM010000016        24605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLTM010000016 DAVLTM010000000
VERSION     DAVLTM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.016"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            142..507
                     /locus_tag="VYN16_000593"
     CDS             142..507
                     /locus_tag="VYN16_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1403165.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            583..1524
                     /locus_tag="VYN16_000594"
     CDS             583..1524
                     /locus_tag="VYN16_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1403166.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHGLSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            1472..2488
                     /locus_tag="VYN16_000595"
     CDS             1472..2488
                     /locus_tag="VYN16_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1403167.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            2490..2816
                     /gene="comGC"
                     /locus_tag="VYN16_000596"
     CDS             2490..2816
                     /gene="comGC"
                     /locus_tag="VYN16_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1403168.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQAKAYKEYHDKNGEA
                     NRKVND"
     gene            2809..3213
                     /locus_tag="VYN16_000597"
     CDS             2809..3213
                     /locus_tag="VYN16_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1403169.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            3218..3478
                     /locus_tag="VYN16_000598"
     CDS             3218..3478
                     /locus_tag="VYN16_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403170.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            3441..3902
                     /locus_tag="VYN16_000599"
     CDS             3441..3902
                     /locus_tag="VYN16_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1403171.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            3880..4293
                     /locus_tag="VYN16_000600"
     CDS             3880..4293
                     /locus_tag="VYN16_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1403172.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEIATDSSEKVEKKKSEEKPEKKENS"
     gene            4430..5024
                     /locus_tag="VYN16_000601"
                     /pseudo
     CDS             4430..5024
                     /locus_tag="VYN16_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            5085..6038
                     /locus_tag="VYN16_000602"
     CDS             5085..6038
                     /locus_tag="VYN16_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1403173.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            6089..7279
                     /locus_tag="VYN16_000603"
     CDS             6089..7279
                     /locus_tag="VYN16_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1403174.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGCSEQQILDIENHAQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            7428..7799
                     /gene="rnpA"
                     /locus_tag="VYN16_000604"
     CDS             7428..7799
                     /gene="rnpA"
                     /locus_tag="VYN16_000604"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1403175.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYQEGNGSEKETKVD"
     gene            7774..8598
                     /locus_tag="VYN16_000605"
     CDS             7774..8598
                     /locus_tag="VYN16_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1403176.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTILIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            8617..9603
                     /locus_tag="VYN16_000606"
     CDS             8617..9603
                     /locus_tag="VYN16_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1403177.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIETISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            9654..10277
                     /locus_tag="VYN16_000607"
     CDS             9654..10277
                     /locus_tag="VYN16_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1403178.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLEKQAKILDIPTVKGIYYLAYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            10408..11865
                     /locus_tag="VYN16_000608"
     CDS             10408..11865
                     /locus_tag="VYN16_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1403179.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            11888..12169
                     /locus_tag="VYN16_000609"
     CDS             11888..12169
                     /locus_tag="VYN16_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1403180.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            12246..12731
                     /locus_tag="VYN16_000610"
     CDS             12246..12731
                     /locus_tag="VYN16_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403181.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            12748..13413
                     /locus_tag="VYN16_000611"
     CDS             12748..13413
                     /locus_tag="VYN16_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1403182.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            13417..14280
                     /locus_tag="VYN16_000612"
     CDS             13417..14280
                     /locus_tag="VYN16_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1403183.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            14282..14986
                     /locus_tag="VYN16_000613"
     CDS             14282..14986
                     /locus_tag="VYN16_000613"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1403184.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            15163..16836
                     /locus_tag="VYN16_000614"
     CDS             15163..16836
                     /locus_tag="VYN16_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1403185.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            16948..18039
                     /gene="ulaG"
                     /locus_tag="VYN16_000615"
     CDS             16948..18039
                     /gene="ulaG"
                     /locus_tag="VYN16_000615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1403186.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            18153..20129
                     /gene="tkt"
                     /locus_tag="VYN16_000616"
     CDS             18153..20129
                     /gene="tkt"
                     /locus_tag="VYN16_000616"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1403187.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            20246..20545
                     /gene="yajC"
                     /locus_tag="VYN16_000617"
     CDS             20246..20545
                     /gene="yajC"
                     /locus_tag="VYN16_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1403188.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            20592..21020
                     /locus_tag="VYN16_000618"
     CDS             20592..21020
                     /locus_tag="VYN16_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1403189.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            21022..21432
                     /locus_tag="VYN16_000619"
     CDS             21022..21432
                     /locus_tag="VYN16_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403190.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            21733..24384
                     /gene="adhE"
                     /locus_tag="VYN16_000620"
                     /gene_synonym="adhC"
     CDS             21733..24384
                     /gene="adhE"
                     /locus_tag="VYN16_000620"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1403191.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTSFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         7697 a         4418 c         5426 g         7064 t
ORIGIN      
        1 atatttttgt acataagtaa atagaaattc agtcatccat cagatggctg gattttttat
       61 caaaaaatta agaaatgagc atatttcttt ccttgtctgg cggaattggt tataatatac
      121 ggtacaaagg aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga ttttgaaccg
      181 tggtggttct tagaaggttg ggaagaagat attgtagcaa gtagaaaatt tgaccagtat
      241 tatgatgctc tcaaatacta caaaacttgc tggtttagat tggaacaaga atcccctctt
      301 tataaaagta gaagcgactt gatgaccatt ttttgggacc cggaagacca acgctggtgt
      361 gatgaatgtg atgagtattt acaacaatac cattctttgg ctcttttgca ggatgagcag
      421 gttatcccag acgaaaaact acgctcaggc tatgaaaaac aaaccagtca ggaaaggaat
      481 cgttcttgcc gtatgaaatt aaaatagaga aaagtaactt ttttggagtt gcttttttta
      541 tttttctaac gctttgcgaa tagtataggt gaggaggtaa gtatggttca agaaattgca
      601 caagaaatca ttcgctcggc tcggaaaaaa ggggcgcaag acatttattt tgtccccaag
      661 ttagacgcct atgagcttca tatgagggta ggagacgagc gctgtaaaat tggtagctat
      721 gattttgaaa agtttgcagc cgttatcagt cactttaagt ttgtggcggg tatgaatgtg
      781 ggagaaaaaa gacgtagtca actgggttcc tgtgattatg cctatgacca gaagatggcg
      841 tctctacgtt tatctactgt aggcgattat cgggggcatg agagtttggt tatccgtttg
      901 ttgcacgatg aggagcagga cttgcatttt tggtttcagg atattgaaga attaggcaag
      961 cagtacaggc aacggggact ctatcttttt gctggtccgg ttggaagtgg taagacgacc
     1021 ttgatgcatg gattgtccaa gtcactcttt aaaggacagc aagttatgtc catcgaagat
     1081 cctgtcgaaa tcaagcagga cgatatgctt cagttgcagt tgaacgaagc aatcggccta
     1141 acctatgaaa atctaatcaa actttccttg cgtcatcgac cagatctctt gattatcgga
     1201 gaaattcgtg acagcgagac ggcgcgtgca gtggtcagag ctagtttgac aggtgcgaca
     1261 gtcttttcaa ccattcacgc caagagtatc cgaggtgttt atgagcgtct gctggagttg
     1321 ggtgtgagtg aagaagaatt ggcagttgtt ctgcaaggag tctgctacca gagattaatc
     1381 gggggaggag gaatcgttga ctttgcaaac agagattatc aagaacacca agcagccaag
     1441 tggaatgagc aaattgacca gcttcttaaa gatggacata tcacaagtct tcaggctgag
     1501 acggaaaaaa ttagctacag ctaagcaaaa aaatatcatc accctattta acaatctctt
     1561 ttctagcggt tttcatctgg tggagactat ctccttttta gataggagtg ctttgttgga
     1621 caagcagtgt gtgatccaga tgcgtgcggg cttgtctcaa gggaaatcat tctcagaaat
     1681 gatggaaagt ttgggatgtt caagtaccat tgtcactcag ttatccctag ccgaagttca
     1741 tggaaatctc cacctgagtt tgggaaagat agaagaatat ctggacaatc tggctaaggt
     1801 caagaaaaaa ttaattgaag tagcgaccta tcctttgatt ttgctgggtt ttcttctctt
     1861 aattatgctg gggctacgga attacctgct cccacaactg gatagtagca atattgccac
     1921 ccaaattatc ggtaatctgc cccaaatttt tctaggcatg gtagggcttg tttccgtgct
     1981 tgccctttta gcactaactt tttataaaag aagttctaag atgagtgtct tttctatctt
     2041 agcacgcctt ccctttattg gaatctttgt gcagacctac ttgacagcct attatgcacg
     2101 tgaatggggg aatatgattt cacagggaat ggagctgacg cagatttttc aaatgatgca
     2161 ggaacaaggt tcccagctct ttaaagaaat cggtcaagat ctggctcaaa ccctgaaaaa
     2221 tggccgtgaa ttttctcaga cgataggaac ctatcctttc tttaggaagg aattgagtct
     2281 catcatagag tatggggaag ttaagtccaa gctgggtagt gagttggaaa tctatgctga
     2341 aaaaacttgg gaagcctttt ttacccgagt caaccgcacc atgaatttgg tgcagccact
     2401 ggtttttatc tttgtggcac tgattatcgt tttactttat gcggcaatgc tcatgcccat
     2461 gtatcaaaat atggaggtaa atttttaaaa tgaaaaaaat gatgacattc ttgaaaaaag
     2521 ctaaggttaa agcttttaca ttggtggaaa tgttggtggt cttgctgatt atcagcgtgc
     2581 ttttcttgct ctttgtacct aatctgacca agcaaaaaga agcagtcaat gacaaaggaa
     2641 aagcagctgt tgttaaggtg gtggaaagcc aggcagaact ttatagcttg gaaaagaatg
     2701 aagatgctag cctaagcaag ttaaaagacg atgggcgaat cacggaagaa caggctaaag
     2761 cttataaaga ataccatgat aaaaatggag aagcaaatcg taaagtcaat gattaaggcc
     2821 tttaccatgc tggaaagtct cttggttttg ggtcttgtga gtatccttgc cttgggcttg
     2881 tccggctctg ttcagtccac ttttgcggcg gtagaggaac agattttctt tatggagttt
     2941 gaagaactct atcgggaaac ccaaaaacgc agtgtagcca gtcagcaaaa gactagtctg
     3001 aacttagatg ggcagacgct tagcaatggc agtcaaaagt tgacagttcc taaaggaatt
     3061 caggcaccat caggccaaag tattacattt gaccgagctg ggggcaattc gtccctggct
     3121 aaggttgaat ttcagaccag taaaggagcg attcgctatc aattatatct aggaaatgga
     3181 aaaattaaac gcattaagga aacaaaaaat tagggcagtg attttactgg aagcagtagt
     3241 cgctctagct atctttgcca gcattgcgac cctccttttg ggacaaattc aaaaaaatag
     3301 gcaagaggaa gcaaaaatct tgcaaaagga agaagtcttg agggtagcta agatggccct
     3361 gcagacgggg caaaatcagg taagcatcaa cggagttgag attcaggtat tttctagtga
     3421 aaaaggattg gaggtctacc atggttcaga acagttgttg gcaatcaaag agccataagg
     3481 tcaaggcttt taccttgtta gaatccctac ttgccctcat tgtcatcagt gggggattac
     3541 tcctttttca aactatgagt cagctcctca tttcagaagt tcgctaccag caacaaagcg
     3601 agcaaaagga gtggctcttg tttgtggacc aacttgaggc agaattagac cgttcgcagt
     3661 tcgaaaaagt agaaggcaat cgcctataca tgaagcaaga tggcaaggac atcgccatcg
     3721 gtaagtcaaa gtcagacgat tttcgtaaaa ccgatgctag tggacggggg tatcagccta
     3781 tggtttatgg cctcaaatca gctcagatta cagaggacaa tcaactggtt cattttcgtt
     3841 tccagtttca aaaaggctta gaaagggagt tcatctatcg tgtggaaaaa gaaaaaagtt
     3901 aaggcaggtg ttctcctcta cgcagtcacc atagcagcca tctttagtct tttgttgcaa
     3961 ttttacttga accgacaagt cgcccactat caagactatg ctttgaataa agaaaaattg
     4021 gttgcttttg caatggctaa acgaaccaag gataaagccg agcaagaaag tggggaacag
     4081 gtctttaatc taggtcaggt aagctatcaa aacaagaaaa caagcttggt gacgactgtt
     4141 cgtacgtcta agagccaata tgagtttctg tttccttcag tcaaaatcaa agaagagaaa
     4201 agagataaaa aggaagagat agcgaccgat tcaagcgaaa aagtggagaa gaaaaaatca
     4261 gaagagaagc ctgaaaagaa agagaattcc tagtcaattc aactataatg cgttgaatcc
     4321 agaatagtcc actatagttt ctataaaatt gctggaaatg gatattaagc tccaattcat
     4381 ttgtttatat cttatttcag tttactatac tttgtgctaa actaaagata tgaaacatga
     4441 ttttaaccac aaagcagaaa ctttcgattc ccctaaaaat atcttcctcg caaacttggt
     4501 atgtcaagca gccgagaaac agattgatct tctatcagac aaagaaattt tagatttcgg
     4561 tggtggcacg ggtctattag ccttgcccct agcccctagc caagcaggct aagtcagtca
     4621 ctcttgtaga catttctgag aaaatgttgg agcaagctcg tttgaaagtg gagcagcaag
     4681 caatcaagaa tatccagttt ttggagcaag atttaccgaa aaatcccttg gagaaagagt
     4741 ttgattgcct tgctgttagt cgggttcttc atcatatgcc tgatttggat gcggctctct
     4801 cactgtttca tcaacatttg aaggaagatg ggaaactcat cattgctgat tttaccaaga
     4861 cagaagctaa tcatcatgga tttgatttag ctgaactgga aaacaagcta attgagcatg
     4921 gtttttcatc tgtgcatagt cagattctct atagcgctga agaccggttt caaggaaatc
     4981 actcagaatt ctttttaata gtagcccaaa aatcactcgc ctagtcaggg agtgattttt
     5041 ctataaggat ggaaaaaaga agggaaattt gataagatag gaatatggat tttgaaaaaa
     5101 ttgaacaagc ttatacctat ttactagaga atgtccaagt catccaaagt gatttggcga
     5161 ccaactttta tgacgccttg gtggagcaaa acagcatcta tctggatggt gaaactgagc
     5221 taaaccaggt caaagacaac aatcaggccc ttaagcgttt agcactacgc aaagaagaat
     5281 ggctcaagac ctaccagttt ctcttgatga aggctgggca aacagaaccc ttgcaggcca
     5341 atcaccagtt tacaccggat gctattgctt tgcttttggt gtttattgtg gaagagttgt
     5401 ttaaagagga ggaaattact atcctcgaaa tgggttctgg gatgggaatt ctaggcgcta
     5461 ctttcttgac ctcgcttgat aaaaaggtgg attacttggg aatggaagtg gatgatttac
     5521 tgattgatct ggcagctagc atggcagatg taattggttt gcaggctggc tttgtccaag
     5581 gagatgccgt tcgtccacaa atgctcaaag aaagcgatgt ggtcatcagt gacttgcctg
     5641 ttggctatta tcctgatgat gccgttgcgt cgcgccatca agttgcttct agccaagaac
     5701 atacttacgc ccatcacttg ctcatggaac aagggcttaa gtacctcaag tcagacggat
     5761 acgctatttt tctagctccg agtgatttgt tgaccagtcc tcaaagtgat ttgttaaaag
     5821 aatggctgaa agaagaggcg agtctggttg ctatgattag tctgcctgaa aacctctttg
     5881 ctaatgccaa acaatctaag actattttta tcttacagaa gaaaaatgaa atagcagtag
     5941 agccttttgt ttatccactt gctagcttgc aagatgcaag tgttttaatg aaatttaaag
     6001 aaaattttca aaaatggact caaggtactg aaatataaaa tagattttgt tataatagtt
     6061 gaaaacgctt aaaaaggggt atcatgttat gacaaaaaca attgcaatca atgcaggaag
     6121 ttcaagtttg aaatggcaat tatacttaat gccagaagaa aaagtattgg cgaaaggttt
     6181 gattgaacgt atcggtttga aagattcaat ttcaactgta aaatttgacg gctgttctga
     6241 acaacaaatt ttggatattg aaaatcatgc acaagccgtt aaaattttat tggatgactt
     6301 gattcgtttc gatattatca aggcttatga cgagattaca ggtgttggac atcgtgttgt
     6361 tgctggtgga gaatatttca aagaatcaac agttgttgag ggagatgttt tagaaaaagt
     6421 tgaagagttg agtttgttgg ctcctctaca caacccggcc aatgcagcag gtgttcgtgc
     6481 cttcaaggaa ttgttgccag acattaccag tgtagttgtt tttgatactt ccttccacac
     6541 aagtatgcca gagaaagctt atcgctaccc tctaccaaca aaatattaca cagaaaacaa
     6601 ggttcgtaaa tacggtgctc atggtacaag tcaccagttt gtagcaggag aggctgcaaa
     6661 actcttggga cgtccattag aagacttgaa gttaattacc tgtcatattg gtaacggagg
     6721 ctcaattaca gctgtgaaag ccggcaaatc tgtagacact tctatggggt tcactcctct
     6781 tggtggtatt atgatgggaa cgcgtacagg ggatattgat ccagctatca ttccttattt
     6841 aatgcaatat acagaggatt ttaacacacc agaagatatc agtcgtgttc ttaaccgtga
     6901 atcaggtctt ttgggagttt ctgctaattc tagcgatatg cgcgatatag aagcagctgt
     6961 agcagaaggg aatcacgagg ctagcttggc ttatgaaatg tatgttgacc gtatccaaaa
     7021 atatatcggt cagtaccttg cagtgctaaa tggagcagat gccattgttt tcacagcagg
     7081 tgtcggtgaa aatgcagaga atttccgtcg tgatgtaatc tcagggattt cgtggtttgg
     7141 ttgtgatgtt gatgatgaaa agaatgtctt tggcgttaca ggagacatct caacagaggc
     7201 agctaaaatc cgtgtcttgg ttattccaac agatgaagaa ttagtcattg cccgtgacgt
     7261 tgaacgcttg aaaaaataag tgaaactaaa aaaatattca atacaaggag ttgggaaagt
     7321 tatttttcca gcttcttttt ctgatgaaat tgtccaaaac cttgctatga ttggcttttt
     7381 tgaaaaatat ggtataatag tagtaattta atagatggag ttgagttttg aagaaaaact
     7441 ttcgtgtaaa aagagagaaa gattttaagg cgattttcaa ggaggggaca agttttgcta
     7501 atcgcaaatt tgtggtctac caattagaaa accagaaaaa ccattttcga gtaggtctat
     7561 cagttagcaa aaaactgggg aatgccgtca ctagaaatca aattaagcga cggattcggc
     7621 atattatcca gaatgcaaaa gggagtctgg tagaagatgt cgactttgtt gtcattgctc
     7681 gaaaaggagt cgaaaccttg ggatacgcag agatggagaa aaatctactc cacgtattaa
     7741 aattatcaaa gatttaccag gaaggaaatg ggagtgaaaa agaaactaaa gttgactagt
     7801 ttgctaggac tgtctctgtt aatcatgaca gcctgtgcga ctaatggggt aactagcgat
     7861 attacagccg aatcggctga tttttggagt aaattggttt acttctttgc ggaaatcatt
     7921 cgctttttat cgtttgatat tagtatcgga gtggggatta ttctctttac gatcttgatt
     7981 cgtacagtcc tcttgccagt ctttcaggtg caaatggtgg cttctaggaa aatgcaggaa
     8041 gctcagccac gcattaaggc gcttcgagaa caatatccag gtcgagatat ggaaagcaga
     8101 accaaactag agcaggaaat gcgtaaagta tttaaagaaa tgggtgtcag acagtcagac
     8161 tctctttggc cgattttgat tcagatgccg gttattttgg ccctgttcca agccctatca
     8221 agagttgact ttttaaagac aggtcatttc ttatggatta accttggtag tgtggataca
     8281 acccttgttc ttccgatttt agcagcagta ttcacctttt taagtacttg gttgtccaac
     8341 aaagctttgt ctgagcgaaa tggcgctacg actgcgatga tgtatgggat tccagtcttg
     8401 atttttatct ttgcagttta tgcgccaggt ggagtcgccc tatactggac agtgtctaat
     8461 gcttatcaag tcttgcaaac ctatttcttg aataatccat tcaagattat cgcagagcgc
     8521 gaggccgtag tacaggcaca aaaagatttg gaaaatagaa aaagaaaagc caagaaaaag
     8581 gctcagaaaa cgaaataaat aaggaggaat ctggtaatgg tagtatttac aggttcaact
     8641 gttgaagaag caatccagaa aggattgaaa gaattagata ttccaagaat gaaggctcat
     8701 atcaaagtca tttctaggga gaagaaaggc tttcttggtc tatttggtaa aaaaccagcc
     8761 caagtggata ttgaaacgat tagtgaaacg actgttgtca aagcaaatca acaggtagta
     8821 aaaggcgttc cgaaaaaaat caatgatttg aacgagcctg tgaagacggt tagtgaagaa
     8881 accgttgacc ttggtcatgt ggttgatgct attaaaaaaa tagaggaaga aggtcaaggc
     8941 atttctgatg aagtcaaggc tgaaatctta aaacatgaaa gacatgccag cactatctta
     9001 gaagaaactg gtcacattga gattttaaat gaacttcaaa tcgaggaagc gatgagggaa
     9061 gaagcaggcg ctgatgacct tgaaactgag caagaccaag ctgaaagtca agaactagaa
     9121 gacttgggct tgaaagttga aacgaacttt gatattgaac aagtagctac ggaagtaatg
     9181 gcttatgttc aaacgattat tgatgacatg gatgttgagg ctacactttc aaatgattat
     9241 aaccgtcgta gcatcaattt gcaaattgat actaacgaac caggtcgtat tatcggctac
     9301 catggtaaag tcttgaaggc cttgcaactg ttggctcaaa attatcttta caactgctat
     9361 tcaagaacct tctatatcac tattaatgtc aatgattatg ttgaacaccg tgcagaagtc
     9421 ttgcagacct atgcgcaaaa attggcgatt cgtgttttag aagaaggacg tagtcatcaa
     9481 acagatccaa tgtcgaatag tgaacgcaag attatccacc gtattatttc acgtatggat
     9541 ggcgtgacca gttactctga aggtgatgag ccaaatcgct atgtcgttgt agatacagaa
     9601 taagtaaaat cagggttgac ctgatttttt gctagctaga ggaggttaaa ctgatgttga
     9661 ataagataag agactatcta gacttttctg gtttgcagta ccgtagtcct gataaagcgg
     9721 gggcagagcg agagaagatg ctggcgttcc gccacaaagg acaagaggcc cgaaaggctt
     9781 ttacagaact ggccaaagcc tttcaagcaa gccatccaga atggcaactc caacagacta
     9841 gtcagtggat gaatcaggcc cagcgtttga gaccacattt ttgggtttat ctacagagag
     9901 acggacaagt gacagaacct atgatggcct tacgtttgta tgggacatct actgactttg
     9961 gaatttcttt ggaagtcagt ttcatcgaac gtaagaagga tgagcaaact ttagagaaac
    10021 aggccaagat tttagacata ccaaccgtta aaggaattta ttatctagcc tactctgatg
    10081 gccaaagtca acggtgggag gcgaacgaag aaaagcgtcg tactttacgc gagaaggtga
    10141 gaagtcaaga agttcgaaaa gttttagtga aggtagatgt tcctatgaca gaaaattcgt
    10201 ctgaagaaga aatcgtagaa ggcttattga agtcttattc taaaattctt ccctattatc
    10261 tagctacgag aaaataagat aatttgtaaa acatcataaa tcatacagtc caagagtgaa
    10321 cagtccgctg tgtaattctt ggtctttttg tttgcgcttt cgcattatat aataaactta
    10381 caaaaacaat tcaaaaggag aacaattatg gaagtcgttt caagtgttct aaattggttt
    10441 tctagcaata ttttgcagaa tcccgcattt ttcgtaggtt tattggtgtt gataggatat
    10501 gcacttttga aaaaacctgc ccatgacgtt ttttcagggt ttgttaaagc aacagtaggg
    10561 tatatgttgc ttaacgtggg tgctggtggt ttggttacaa cctttcgtcc aatcttagca
    10621 gctcttaact acaaattcca aattggtgca gcggttatcg acccttactt tggacttgct
    10681 gcagcaaaca acaaaattgc agcagagttt ccagattttg ttggaactgc aactacagct
    10741 ctattgattg gttttggaat aaatatcttg ctcgtagctc ttcgaaagat tacgaaggta
    10801 agaaccctct ttattactgg tcacatcatg gtacaacaag ctgcaacagt atctcttatg
    10861 gttctattct tagtaccaca attgcgcaat gcttacggta cagcagcgat tggtatcatc
    10921 tgtggacttt actgggcagt tagttcaaat atgactgttg aggcaactca acgcttgact
    10981 ggtggtggcg gatttgcgat tggtcaccaa cagcaatttg caatctggtt tgtagataaa
    11041 gtagcaggac gctttggtaa gaaagaagaa agtttagaca atcttaaatt acctaagttc
    11101 ctctcaatct tccacgatac agttgttgca tctgctacct tgatgctcgt attcttcggg
    11161 gccattcttt taatcttggg tccagacatt atgtctaata aagaagtcat cacttcagga
    11221 actctattca atcctgctaa acaagatttc tttatgtaca ttatccaaac agcctttacc
    11281 ttctcagttt acttgttcgt tttgatgcaa ggtgtccgaa tgttcgtatc tgagttaaca
    11341 aacgctttcc aaggtatttc aaacaaattg ttgccaggtt cattcccagc ggttgacgtt
    11401 gcagcttctt atggatttgg ttctccaaat gctgtcttgt caggatttac ctttggtttg
    11461 attggtcaat tgattacaat tgtcttgctc atcgtcttta aaaatccgat tcttattatt
    11521 acaggatttg taccagtgtt ctttgacaat gcagccattg cggtctacgc tgataaacgc
    11581 ggcggatgga aagcggctgt tatcctttcc tttatatcag gtgtccttca agttgctcta
    11641 ggagctcttt gtgtggccct tctcgatttg gcatcttatg gtggctacca tggaaatatc
    11701 gactttgaat tcccatggct tggatttgga tatatcttca aataccttgg tattgttggt
    11761 tatgtacttg tgtgtctctt cttgcttgtt attcctcaac ttcaatttgc caaagcaaaa
    11821 gataaagaga aatattacaa cggtgaagtt caagaagaag cttagtatct agaaaaggag
    11881 aaataaaatg gttaaagtat tagcagcgtg cggaaatgga atgggttcat caatggttat
    11941 caagatgaag gttgaaaatg ctctccgtaa gcttaatcaa acagatttta cagtcaattc
    12001 atgcagtgtc ggtgaagcta aaggtttagc agtaggatat gacatcgtaa tcgcttctct
    12061 tcatttgatt caagaattgg aagggcgaac taatgggaag ttaattggac ttgataactt
    12121 gatggatgat aaagaaatca ccgaaaaact cagtcaagca ctacagtaaa aggttggagg
    12181 gggctggaca gaaactgaga gttatcgttt ctgtccttct ccctctttaa ataaaggagg
    12241 cagatatgaa tttaaaacaa gctttaattg acaatgactc gatccgacta ggtttagagg
    12301 ctaacgattg gaaagaagca gtcaaggtag cagtagatcc cttgattgaa agtggggcaa
    12361 ttttgccaga gtattacgat gctatcattg aatcgactga agagtatggg ccttactata
    12421 tcttgatgcc aggtatggct atgccccacg ctagacctga agctggtgtg caaagtgatg
    12481 ccttttcatt aattacctta caaaatcctg ttgtattttc agatgggaaa gaggtatctg
    12541 ttttgttggc actagcagca acaagttcaa aaattcacac aagtgtagcc attccacaaa
    12601 ttattgccct gtttgaatta gaagattcta ttgcacgttt acaggcttgc cagactaaag
    12661 aagatgtctt ggctatgatt gaagaatcta aggatagccc ttatctcgaa ggattggatt
    12721 tggaaagtta gaaagaggaa taaagaaatg acaaaaagaa tacctaattt acaagttgca
    12781 ttagaccatt cagacttgca aggagcgatt aaagcagctg tttctgttgg tcaggaagta
    12841 gatattatcg aagctggaac tgtttgcttg cttcaagttg gaagtgaact ggctgaagtc
    12901 ttgcgtagcc ttttcccaga taagattatt gtggcagaca caaaatgtgc tgatgctggt
    12961 ggaacagttg ctaaaaataa tgcggttcgt ggagcagact ggatgacttg tatctgttgt
    13021 gcaaccatcc ctactatgga agcagctcta aaggctatca agactgaacg aggagaacga
    13081 ggcgaaatcc agatcgagct ttatggcgat tggacttttg aacaagctca gctttggcta
    13141 gatgcaggta tctcacaagc tatttatcac caatctcgtg atgctcttct tgctggtgaa
    13201 acttggggtg aaaaagacct taataaggtt aaaaaactca ttgacatggg cttccgtgta
    13261 tctgtaacag gtggtctaga tgtagatact ctcaaactct ttgaaggtgt tgatgtcttt
    13321 acctttatcg caggtcgtgg aattacagag gctgtggatc cagcaggagc agcgcgtgcc
    13381 ttcaaggatg aaatcaaacg aatttggggg taaatcatgg tacgtccaat tggaatttat
    13441 gaaaaggcaa ccccaacaca ctttacttgg ctagaacgtt taaattttgc caaggagtta
    13501 ggctttgatt ttatcgagat gtctattgac gaacgtgacg agcgtttagc aagacttgac
    13561 tggagtaagg aagaacgctt ggaagttgtc aaagaaatct atgaaactgg tgttcgtatt
    13621 ccttctatct gtttttcagg ccatcgtcgc tacccattgg gatcaaaaga ttcagttcta
    13681 gaggaaaaat ctctagaact catgaaaaaa tgtatcgaat tagctcaaga cttgggagtt
    13741 cgtacgattc aattagctgg ttacgatgtt tactatgagg aaaagtcacc ccagacacgc
    13801 caacgtttta tcaaaaattt gagaaaagcc tgtgactggg ctgaagaagc tcaggtggta
    13861 cttgctattg aaattatgga tgatcctttc atcaatagca tcgaaaaata tttggctata
    13921 gaaaaagaga ttgactctcc cttcctcttt gtatatccag atattggtaa tgtgtctgca
    13981 tggcataatg atatctatag tgagttttat cttggtcatc atgccatcgc agctctccat
    14041 ctcaaggata cttatgcagt gacagaaagt tcaaagggcc agttccgaga tgtacctttt
    14101 gggcaaggtt gtgtcaaatg ggaagaagct ttcgatattt taaaggaaac caattataat
    14161 ggacctttcc taatcgaaat gtggtctgaa aattgtgaaa cagtagaaga aacacgcgca
    14221 gccattcaag aggcgcaagc ttttctctat ccactcatta agaaagcagg tttgatgtaa
    14281 gatgaatcaa gtaatcaatg ctatgcgtaa acgagtctgt gatgccaatc aatcattgcc
    14341 aaaacatgga cttgtcaaat ttacctgggg gaatgtatct gaagtcaatc gcgaactcgg
    14401 tgtcattgtt atcaaaccat caggcgtgga ttatgacgaa ttgacacctg aaaacatggt
    14461 agtgactgat ctagatggta agatcctaga aggggattta agaccatctt ccgacctccc
    14521 aactcatgtg caattatata aggcttggtc agaaattggt agtgtggttc acacccattc
    14581 gacagaagct gttgcttggg ctcaggcagg tcgtgatatt cctttctacg gaacaaccca
    14641 tgcagattat ttctacggtt caatcccttg cgcccgtagt ttgaccaagg acgaagtaga
    14701 agtggcctat gaaaaagata ctggcctggt tatcgtagaa gagtttgaac atcgcggact
    14761 taacccggtt gaagtaccag gaattgttgt acgcaatcac ggtccattca cctggggcaa
    14821 aaatccagag aatgctgttt atcactctgt cgtactagag gaagtatcaa agatgaatcg
    14881 ctttacagaa caaatcaatc caagagttga acctgctccc cagtacatac tagaaaaaca
    14941 ctaccaacgt aaacatggac caaatgctta ttatggtcaa aagtaagaac gatgaaggag
    15001 gagaaaaaga taaatttagc tcctcttttt acatttgatt tttattgaga gtaaagttgg
    15061 agttgaagta attttgaaag attttttaga aatagcgctt gatatatatg gtaaaataaa
    15121 aagaattgct gtgatatcaa tagatttggg ggatttttta atatggtact ggataaggca
    15181 agttgtgatt tgcttcaata tttgatggat caagaaacgt ccaaaacgat tatggcgatt
    15241 tcgaaagatt tgaaagagtc aagaaggaaa atttattatc acattgacaa aatcaatgct
    15301 gctctggttg acgaggcgct tcacatcatt agtattccac gaattggtat tcacttaacg
    15361 gaagagcaga gagatgcttg ttgtaaacta ttatcggaag tagattcgta cgattatatc
    15421 atgagtgcgc atgaacgtat gatgataatg ttactatgga taggtatttc taaagaacgt
    15481 attactattg aaaaattgat agagttaaca gaggtatcta ggaatactgt tctcaatgat
    15541 ttgaatagta ttcgttatca actaactttg gaacaatatc aggtgacctt gcaagtgagc
    15601 aagtcacagg gataccacct tcatgcccac cctcttaata aaattcagta tcttcaatcg
    15661 cttctatatc atatttttat ggaagaaaat gccacttttg tatctatttt agaagataag
    15721 atgaaagaga ggttagatga tgagtgtttg ctttctgttg aaatgaacca attttttaag
    15781 gaacaggttc ctttagttga acaagattta gggaagaaaa taaaccatca tgaaataact
    15841 tttatgttgc aggttctacc ttatttgctg ttaagctgtc ataatgttga acagtatcaa
    15901 gaaagacatc aggatataga gaaagaattt tctttgataa gaaaaagaat agagtatcag
    15961 gtgtctaaga aattaggaga acggttgttt caaaagtttg aaatttcttt gtcaggactt
    16021 gaagtttctc ttgtagctgt tctcctcctc tcctatcgta aagatttgga tattcatgca
    16081 gaaagtgatg attttcggca attaaaactt gctttagaag aatttatctg gtattttgaa
    16141 tcacaaatcc gaatggagat tgagaacaag gatgatttgt tacgaaattt gatgatccac
    16201 tgtaaagcct tgttatttag aaagacttac ggtatttttt ctaaaaatcc tctaacaaaa
    16261 caaattcgat ccaagtatgg agaattattt ttagtcacta gaaaatctgc ggaaatttta
    16321 gaaggagcat ggtttattcg gctaacagac gatgatattg cctatttgac gattcatatt
    16381 ggaggatttt taaagtatac accatcgtct caaaaaaata tgaaaaaagt ttatctcgtt
    16441 tgtgatgaag gtgttgcggt ttcgagactt ttgctgaaac aatgcaaact ttattttcca
    16501 aatgagcaaa ttgacactgt atttacaaca gaacaattta agagtgtgga agatattgca
    16561 caagttgatg tagtgattac tactaatgat gatttggata gcagatttcc gattttaagg
    16621 gttaatccta tccttgaagc agaagatatt ttgaaaatgc tagactatct taaacacaat
    16681 atatttcgta ataagagcaa aagtttcagt gaaaatcttt ctagtcttat ttcgtcttat
    16741 attgtagaca gcaagttggc tagtaagttc caagaagagg ttcaaacact tataaataaa
    16801 gaaatagtag ttcaagcttt tttggaagat atttgaagga cagtccaatg atgaacacaa
    16861 acctgtgttt ttcttggtct tttttagtgt tttgaagggt ggtatactaa tctcaaagat
    16921 aacaattata tccaaaggag gcaacatatg ccaaacgtca aagaaattac aagagagtca
    16981 tggattttag ccactttccc agagtgggga acatggttga acgaagaaat cgaagaagaa
    17041 gtcgtacctg aaggcaactt tgccatgtgg tggctaggca actgtggtac ttggattaag
    17101 acaccagctg gtgctaacgt tgtcatggac ctttggtcaa accgtggaaa atcaaccaaa
    17161 aaagtgaaag atatggttcg tgggcaccaa atggcaaata tggcaggtgt tcgtaagttg
    17221 caaccaaact tgcgtgttca gccaatggtt atcgatccat ttgctatcaa cgaactagac
    17281 tattacttag tttcacactt ccacagtgat catatcgacc catacacagc tgcagcaatt
    17341 ctcaacaatc ctaagttaga gcatgttaag tttatcggtc cttaccactg tggacgaatc
    17401 tgggaaggat ggggtgttcc aaaagaacgt atcatcgttg ttaaaccagg tgacactatc
    17461 gaattaaaag atatgaagat tcatgcagta gaatcatttg accgtacttg cttggtaact
    17521 ctcccagtga acggtgctga tgagacaggc ggtgaacttg ctggcttggc tgttacagat
    17581 gaagaaatgg ctcaaaaggc tgttaactat atctttgaaa caccaggtgg aaccatctat
    17641 catggtgcag attctcactt ctcaaactat tttgcaaaac atggtaaaga ctttaaaatt
    17701 gatgttgctt tgaataacta tggtgaaaat ccggtaggta tccaagacaa aatgacatct
    17761 atcgaccttc ttcgtatggc agaaaatctg cgtaccaaag tcattatccc agttcactat
    17821 gatatctggt ctaacttcat ggcttctact aatgagattc tagaactttg gaaaatgcga
    17881 aaagatcgct tgcaatacga tttccatcca tttatctggg aagttggcgg taagtacact
    17941 tatcctcaag atcaacactt agtagaatac catcatccac gtggttttga tgattgtttt
    18001 gaacaagact ctaacattca atttaaagct ttgctataat ataaaaatat tctttggagg
    18061 ttatccgtat gttgcatgat acggataatt ttcatataat agtaaaatag aatgtgtgat
    18121 tcaataatca cctcaaatag aaaggaaatt ctatgtcaaa tctatctgtt aatgcaattc
    18181 gttttctagg tattgacgcc attaataaag ccaactcagg tcatccaggt gtggttatgg
    18241 gagcggctcc gatggcttac agcctcttta caaaacaact tcatatcaat ccagctcaac
    18301 caaactggat taaccgcgac cgctttattc tttcagcagg tcatggctca atgctccttt
    18361 atgctcttct tcacctttct ggttttgaag atgtcagcat ggatgagatt aagagcttcc
    18421 gtcaatgggg ttcaaaaaca ccaggtcacc cagaatttgg tcatacggca gggattgatg
    18481 ctacgacagg tcctctaggg caagggattt caactgctac tggttttgcc caagcagaac
    18541 gtttcttggc agccaaatat aaccgtgaag gctacaatat ctttgaccac tatacttacg
    18601 ttatctgtgg agacggagac ttgatggaag gtgtctcaag cgaggcagct tcatacgcag
    18661 gcttgcaaaa acttgataag ttggttgttc tttatgattc aaatgatatc aacttggatg
    18721 gtgagacaaa ggattccttt acagaaagtg ttcgtgaccg ttacaatgcc tacggttggc
    18781 atactgcctt ggttgaaaat ggaacagact tggaagccat ccatgctgct atcgaaacag
    18841 caaaagcttc aggcaagcca tctttgattg aagtgaagac ggttattgga tacggttctc
    18901 caaacaaaca aggaactaat gctgtacacg gcgcccctct tggagcagat gaaactgcat
    18961 caactcgcca agccctcggt tgggactacg aaccatttga aattccagaa caagtatatg
    19021 ctgatttcaa agaacatgtt gcagaccgtg gcgcatcagc ttatcaagct tggactaaat
    19081 tagttgcaga ttataaagaa gctcatccag aactggctgc agaagtagag gccatcatcg
    19141 acggacgtga tccagtcgaa gtgactccag cagacttccc agctttagaa aatggttttt
    19201 ctcaagcaac tcgtaattca agtcaagatg ccttgaatgt tgtggcagct aaattaccaa
    19261 ccttcctagg tggatcagct gaccttgctc actcaaacat gacttatatc aagacggatg
    19321 gacttcaaga cgataccaat cgcttgaatc gtaacattca gtttggtgtt cgtgaatttg
    19381 caatgggaac aatcttgaac gggatggccc ttcatggtgg acttcgtgta tacggtggaa
    19441 ctttcttcgt cttctctgac tatgtgaagg cagctgtccg cttgtcagcc ttacaaggac
    19501 ttcctgtgac ttatgtcttt acccatgatt caatcgcagt tggggaagat ggtccgactc
    19561 acgaaccagt tgagcattta gcaggtcttc gtgctatgcc aaatctaaat gttttccgtc
    19621 cagcagatgc gcgtgaaact caagcagctt ggtaccttgc agtgacaagt gagaaaacac
    19681 caactgccct tgtcttgaca cgtcaaaact tgactgttga agatggaaca gacttcgaca
    19741 aggttgctaa aggtgcttat gttgtatatg aaaatgcagc cgactttgat accatcttga
    19801 ttgcgacagg ttcagaggtt aatcttgctg tctcagctgc taaagaattg gctagtcaag
    19861 gcgaaaaaat ccgcgtagtc agcatgccat ctacagatgt ctttgataaa caagatgcag
    19921 cttacaagga agaaattctt ccaaatgcag tccgccgtcg tgttgcagtc gaaatgggtg
    19981 caagtcaaaa ctggtacaaa tatgttggtc tcgatggtgc cgttctaggt attgatacct
    20041 tcggagcctc tgccccagca ccaaaagtat tggcagaata tggctttact gtagaaaatc
    20101 ttgtaaaaat tgttcgaaac ttgaaataat cctaaaaatc agggcgtaag ctctggtttt
    20161 tcttaccaga aaagtaaggt acaatcttgt aaaagtagct gaaatttgat atagtagtcc
    20221 tatgtaaaag acaaaggaga aaacaatgaa tccaaatatt acttttttaa tcatgcttgt
    20281 aggtatgatg gccttgatgt tctttatgca acgttctcaa aagaaacaag ctcaaaagcg
    20341 tatggaaagc ttaaataaac tacaaaaagg ctatgaagtg attacaatcg gtggacttta
    20401 tggaacagtc gatgaagtag atacggagaa gggaacaata gttcttgacg tagatggagt
    20461 ttacttgact tttgaactag ctgctatcaa gacagtatta ccgctgaaag aaacagcttc
    20521 actagaaggc gcaattgaaa aataagacgg gatcacgaac tcccgttttt ctataaaaga
    20581 aaggaaatgg gatgaaaaaa ttagtctttg tctgtctggg aaatatttgc cgtagcccta
    20641 tggccgagtt tgttatgaaa tcaatgacag ataactacga aatccaaagt cgagcaactt
    20701 cctcttggga acatggcaat ccgattcata aggggactca gggaattttt caacagtatg
    20761 agattcctta tgacaagaac aagacatcgc ttcagattag taaggaagat tttgaagcct
    20821 ttgattatat tatcggaatg gacgcttcaa atatttctga cttacgtcag atgtgtccag
    20881 tagactgtca agataagatt tactcatttt catctgaaag tgttccagac ccttggtata
    20941 caggagattt tgaagaaacc tatcgacgtg ttcaagaggg ctgtcaagct tggttagaac
    21001 gtttagaaaa ggagagttaa ggtggaaaat cttacgaatt tttacgaaaa gtatcgtgtc
    21061 tatctgactc gtccacgttt agagcttttg gcagtagtta ccattgtttt ctgtgctgta
    21121 ctcgtctttt ttctaaatat tccaggaaaa ggtgtcttaa aactcgataa tggaacgatt
    21181 gtttatgatg gcagtcttgt ccgtggtaaa atgaatggtc aaggtaccat taccttccaa
    21241 aatggagacc aatatacagg tggcttcaac aatggagcct tcaacggaaa aggtaccttt
    21301 caatctaaag aaggctggac ctacgaaggt gattttgtaa atggtcaggc tgaaggaaaa
    21361 gggaaactaa caacagaaca agaagtcgtt tatgaaggaa cttttaaaca aggcgttttt
    21421 caacaaaaat aaagcctcct tatccaaagg aggtattatt agaaccacaa ggtaagcgct
    21481 tacctgtaaa tccctttctt tccaaatccc tcttccaagc aagtttgtga aataaaaaat
    21541 atttgaaata aatttcacaa acttcaaaga taaaacctga taagaaaaga aaatgagaaa
    21601 agtttcacaa gagtttaaaa attctttgtg aaatgtttat gaaactgttt acaaagttga
    21661 aataatgcgt tataataaac ttgtgaaaaa attaacaaag gatatcgttc cttgaaagct
    21721 atggaggaaa atatggctga taaaaaaact gtgacaccag aggaaaagaa actcgttgct
    21781 gaaaaacacg tagatgagtt ggttcaaaaa gctctagttg cccttgaaga aatgcgtaaa
    21841 ttggatcaag agcaagttga ctacatcgtt gccaaagcat cagtagcagc tttggatgcc
    21901 cacggagaat tggctttaca tgcctttgaa gaaacaggac gtggtgtatt tgaagacaaa
    21961 gcaactaaga acttgtttgc ctgtgaacac gtagtaaaca acatgcgcca cactaagaca
    22021 gttggcgtta tcgaagaaga cgatgtaaca ggattgactc ttattgctga accagttggt
    22081 gttgtttgtg gtattactcc aacaacaaac ccaacatcaa cagcaatttt caaatcattg
    22141 atttcattga agacacgtaa cccaatcgtc tttgccttcc atccatcagc acaagaatca
    22201 tctgctcatg cagctcgtat cgtccgcgat gcagctatcg cagctggtgc tcctgaaaac
    22261 tgtgtgcaat ggattactca accatctatg gaagcaacaa gtgcccttat gaaccacgaa
    22321 ggggttgcga caatccttgc aacaggtggt aatgccatgg ttaaggcggc ttattcatgt
    22381 ggtaaaccag ctcttggggt aggtgccgga aacgttccag cttatgttga aaaatcagca
    22441 aacattcgtc aagcagcaca cgatatcgtc atgtctaaat catttgataa cggtatggtc
    22501 tgtgcatctg aacaagcagt tatcattgat aaagaaattt acgatgaatt tgtagcagag
    22561 ttcaaatctt accacactta ctttgtaaac aaaaaagaaa aagctcttct tgaagagttc
    22621 tgcttcggcg tcaaagcaaa cagcaaaaac tgtgctggtg caaaattgaa cgctgacatc
    22681 gttggtaaac cagcaacttg gattgcagaa caagcaggat ttacagttcc agaaggaaca
    22741 aacattcttg ctgcagaatg taaagaagtt ggcgaaaatg agccattgac tcgtgaaaaa
    22801 ttgtcaccag ttattgcagt tttgaaatct gaaagccgtg aagatggtat tactaaggct
    22861 cgtcaaatgg ttgaatttaa cggtcttgga cactcagcag ctatccacac agctgacgaa
    22921 gaattgacta aagaatttgg taaagctgtt aaagctattc gtgttatctg taactcacct
    22981 tctacttttg gtggtatcgg ggacgtttac aatgccttct tgccatcatt gacacttgga
    23041 tgtggttctt acggacgcaa ctcagttggg gataacgtta gtgccattaa cctcttgaat
    23101 atcaaaaaag tcggaagacg gagaaataac atgcaatgga tgaaacttcc ttcaaaaaca
    23161 tactttgaac gtgattcaat tcaatacctt caaaaatgtc gtgacgttga acgtgtcatg
    23221 atcgttactg accatgccat ggtagagctt ggtttccttg atcgtatcat cgaacaactg
    23281 gaccttcgtc gcaataaggt tgtttaccaa atctttgcgg atgtagaacc ggatccagat
    23341 atcacaactg taaaccgtgg tactgagatt atgcgtgcct tcaaaccaga taccatcatc
    23401 gcactcggtg gtgggtctcc aatggatgct gccaaagtaa tgtggctctt ctacgagcaa
    23461 ccagaagtgg acttccgtga ccttgtccaa aaattcatgg atatccgtaa acgtgccttc
    23521 aagttcccat tgcttggtaa gaagactaaa ttcatcgcga ttccaactac atctggtaca
    23581 ggatctgaag taacatcatt tgccgttatc tctgataaag caaacaaccg taaataccca
    23641 atcgctgact actcattgac accaactgtg gcaatcgtag atcctgcttt ggtattgaca
    23701 gttccaggat ttgttgctgc tgatactggt atggacgtat tgactcacgc gacagaagca
    23761 tacgtatcac aaatggctag tgactacact gatggtttag cacttcaagc cattaaattg
    23821 gtctttgaaa atctcgaaag ctcagttaag aatgcagact tccactcacg tgagaaaatg
    23881 cataacgctt caacaatcgc tggtatggcc tttgccaatg ccttcctagg tatttctcac
    23941 tcaatggccc ataagattgg tgcgcaattc cacacaatcc acggtcgtac aaatgctatc
    24001 ttgcttccat acgttatccg ttacaacggt acacgtccag ctaagacagc aacatggcct
    24061 aagtacaact actaccgtgc agatgaaaaa taccaagata tcgcacgcat gcttggactt
    24121 ccagcttcta ctccagaaga aggggttgaa tcttacgcaa aagctgtcta cgaactcggt
    24181 gaacgtattg ggatccaaat gaattttaga gaccaaggaa ttgacgaaaa agaatggaaa
    24241 gaacattctc gtgaattagc cttcctggct tatgaagacc aatgttcacc agctaaccca
    24301 cgtcttccaa tggtagacca tatgcaagaa atcatcgaag atgcatacta tggctacaaa
    24361 gaaagaccag gacgccgtaa ataattgttt atcagtctag aagcaagaca aaaactcaat
    24421 ttgagggaaa gatccagtaa tttttctatg ataaaaggca tcctatcaag gtttttgaac
    24481 acctgatagg atgccttttt atgatattga ggcctttttg ccctttttga aaaactagaa
    24541 tagaaacaaa atatataata gattgaaact agagtagtac acatctgctt ctaaaacatt
    24601 gttag
//
LOCUS       DAVLTM010000017        24158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLTM010000017 DAVLTM010000000
VERSION     DAVLTM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.017"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            592..2745
                     /locus_tag="VYN16_000621"
     CDS             592..2745
                     /locus_tag="VYN16_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1403192.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAALSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2758..4107
                     /locus_tag="VYN16_000622"
     CDS             2758..4107
                     /locus_tag="VYN16_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1403193.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTVKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAEINLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4277..4984
                     /locus_tag="VYN16_000623"
     CDS             4277..4984
                     /locus_tag="VYN16_000623"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1403194.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5186..8911
                     /locus_tag="VYN16_000624"
     CDS             5186..8911
                     /locus_tag="VYN16_000624"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1403195.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLA
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNRVKLDGHKLDSAFQGTLEEVYPTEFTRAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9004..10446
                     /locus_tag="VYN16_000625"
     CDS             9004..10446
                     /locus_tag="VYN16_000625"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1403196.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10483..11505
                     /locus_tag="VYN16_000626"
     CDS             10483..11505
                     /locus_tag="VYN16_000626"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1403197.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11502..12047
                     /gene="purN"
                     /locus_tag="VYN16_000627"
     CDS             11502..12047
                     /gene="purN"
                     /locus_tag="VYN16_000627"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1403198.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12131..12640
                     /locus_tag="VYN16_000628"
     CDS             12131..12640
                     /locus_tag="VYN16_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1403199.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITNLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12665..14212
                     /gene="purH"
                     /locus_tag="VYN16_000629"
     CDS             12665..14212
                     /gene="purH"
                     /locus_tag="VYN16_000629"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1403200.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14334..15596
                     /gene="purD"
                     /locus_tag="VYN16_000630"
     CDS             14334..15596
                     /gene="purD"
                     /locus_tag="VYN16_000630"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1403201.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQLILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLDYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15999..16487
                     /gene="purE"
                     /locus_tag="VYN16_000631"
     CDS             15999..16487
                     /gene="purE"
                     /locus_tag="VYN16_000631"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1403202.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16474..17565
                     /gene="purK"
                     /locus_tag="VYN16_000632"
     CDS             16474..17565
                     /gene="purK"
                     /locus_tag="VYN16_000632"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1403203.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIETC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            17575..17802
                     /locus_tag="VYN16_000633"
     CDS             17575..17802
                     /locus_tag="VYN16_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1403204.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17865..19163
                     /gene="purB"
                     /locus_tag="VYN16_000634"
     CDS             17865..19163
                     /gene="purB"
                     /locus_tag="VYN16_000634"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1403205.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19218..23156)
                     /gene="strH"
                     /locus_tag="VYN16_000635"
     CDS             complement(19218..23156)
                     /gene="strH"
                     /locus_tag="VYN16_000635"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1403206.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMDFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQEEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23472..>24158)
                     /locus_tag="VYN16_000636"
     CDS             complement(23472..>24158)
                     /locus_tag="VYN16_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1403207.1"
                     /translation="DELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAK
                     DGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLR
                     GDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMND
                     EHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYK
                     IKFISCDRDH"
BASE COUNT         6577 a         4802 c         5750 g         7029 t
ORIGIN      
        1 atcaaaacta taaatgatga atctgaatca aaattatttt gcgcatgtaa agaggagtct
       61 tgcagtaacg aatcaaaaaa aaggagtaac tatgaatcgt aatttagaat ggtgttatct
      121 attatgacta taaatagatc ataccagagg tggcttagaa atagcaggga cattagaaat
      181 tgaagtaact ttaaatagga tgtcgtaaat gttgctatca atgatttatt tgtcccaagc
      241 ttgcctaggg tgtcagtaaa aaatcatttt cctttcatac catattttta gtaggtagaa
      301 cgtttgttct gcctattttt ttatccaaaa agtgcagttg ggagggagat aggctcattt
      361 gggaaggaag tccagttttt gtttagtgat tggggtaaga tagttgttat cagatgagtt
      421 tatactcttc gaaaatcaaa ttcaaaccac gtcaacgtcg ccttgccgta tatatgtgac
      481 tgacttcgtc agtcctatct acaacctcaa aacagtgttt tgagcagcct gcggctagtt
      541 tcctagtttg ctctttgatt ttcattgagt attagggaaa aggagatgaa tatgaaattt
      601 gggaaacgtc actatcgtcc gcaagtggat cagatggact gcggtgtagc ttcattagcc
      661 atggtttttg gctactatgg tagttattat tttttggctc acttgcgaga attggctaag
      721 acgaccatgg atgggacgac ggctttgggc ttggtcaagg tggcagagga gattggcttt
      781 gagacacgag ctatcaaggc ggatatgacg ctttttgatt tgcccgattt gacttttcct
      841 tttgttgccc atgtgcttaa ggaagggaaa ttgctccact actatgtggt gactgggcag
      901 gataaggaca gcattcatat tgccgatcca gatcctgggg tgaaattgac caaactgcca
      961 cgtgagcgtt ttgaggaaga atggacagga gtgactctgt ttatggcacc tagtccagac
     1021 tacaagcctc ataaggatca aaagaatggt ctgctctctt ttatccctat attagtgaag
     1081 cagcgtggct tgattgccaa tatcgttttg gcaacactct tggtaacctt gattaacatt
     1141 gtgggttctt attatctgca gtctatcatt gatacctatg tgccagatca gatgcgttcg
     1201 acgttgggga ttatttctat tgggctagtc atcgtctaca tcctccagca aatcttgtct
     1261 tacgctcagg agtatctctt gcttgttttg gggcaacgct tgtcgattga cgtgattttg
     1321 tcctatatca agcatgtttt tcacctccct atgtccttct ttgcgacacg caggacaggg
     1381 gagatcgtgt ctcgttttac agatgctaac agtatcatcg atgcgctggc ttcgaccatc
     1441 ctttcgattt tcctagatgt gtcaacggtt gtcattattt cccttgtttt attttcacaa
     1501 aataccaatc tctttttcat gactttattg gcgcttccta tctacacagt gattatcttt
     1561 gcctttatga agccatttga aaagatgaat cgggacacca tggaagccaa tgcggctctg
     1621 tcttcttcta tcattgagga catcaatggt attgagacta tcaagtcctt gaccagtgaa
     1681 agtcagcgtt accaaaaaat tgacaaggaa tttgtggatt atctgaagaa atcctttacc
     1741 tatagtcgag cagagagtca gcaaaaggct ctgaaaaagg ttgcccatct cttgcttaat
     1801 gtcggcattc tctggatggg ggctgttctg gtcatggatg gcaagatgag tttggggcag
     1861 ttgattacct ataatacctt gctggtttac tttaccaatc ctttggaaaa tatcatcaat
     1921 ctgcaaacca agcttcagac agcgcaggtt gccaataacc gtctaaatga agtgtatcta
     1981 gtagcttctg agtttgagga gaagaaaaca gttgaggatt tgagcttgat gaagggagag
     2041 atgactttca agcaggttca ttacaagtat ggctatggtc gagacgtctt gtcggatatc
     2101 aatttaaccg ttccccaagg gtctaaggtg gcttttgtgg ggatttcagg gtcaggtaag
     2161 acgactttgg ccaagatgat ggttaatttt tacgacccaa gtcaagggga gattagtctg
     2221 ggtggtgtca atctcaatca gattgataaa aaagccctgc gccagtacat caactatctg
     2281 cctcaacagc cctatgtctt taacggaacg attttggaga atcttctttt gggagccaag
     2341 gaggggacga cacaggaaga tatcttacgg gcggtcgaat tggcagagat tcgagaggat
     2401 atcgagcgca tgccactgaa ttatcagaca gaattgactt cggatggggc agggatttca
     2461 ggtggtcaac gtcagagaat cgctttggcg cgtgctctct tgacagatgc gccggtcttg
     2521 attttggatg aggcgactag cagtttggat attttgacag agaagcggat tgtcgataat
     2581 ctcatggctt tggacaagac cttgattttc attgctcacc gcttgactat tgctgagcgg
     2641 acagagaagg tagttgtctt ggatcagggc aagattgtcg aagaaggaaa gcatgctgat
     2701 ttgcttgcac agggtggctt ttacgcccat ttggtcaata gctagaaaga ggagaggatg
     2761 aaaccagaat ttttagaaag tgcggagttt tataatcgtc gttaccataa tttttccagt
     2821 agtgtgattg tacccatggc ccttctgctc gtgtttttac ttggctttgc aactgttgca
     2881 gaaaaggaga tgagtttgtc cactagagct actgtcgaac ctagtcgtat ccttgcaaat
     2941 atccagtcaa ctagcaacaa tcgtattctt gtcaatcatt tggaagaaaa taagctggtt
     3001 aagaaggggg atcttttggt tcaataccaa gaaggggcag agggtgtcca agcggagtcc
     3061 tatgccagtc agttggacat gctaaaggat caaaaaaagc aattggagta tctgcaaaag
     3121 agcctgcaag aaggggagaa ccactttcca gaggaggata agtttggcta ccaagccacc
     3181 tttcgcgact acatcagtca agcaggcagt cttagggcta gtacatcgca acaaaatgag
     3241 accatcgcgt cccagaatgc agcagctagc caaacccaag ccgaaatcgg caacctcatc
     3301 agtcaaacag aggctaaaat tcgcgattac cagacagtta agtcagctat tgaaacaggt
     3361 gcttccttgg ccggtcagaa tctagcctac tctctttacc agtcctacaa gtctcagggc
     3421 gaggaaaatc cccaaactaa ggttcaggca gttgcacagg ttgaagcaca gatttctcag
     3481 ttagaatcta gtcttgctac ttaccgtgtc cagtatgcag gttcaggtac ccagcaagcc
     3541 tatgcgtcag ggttaagcag tcaattggaa tcccttaaat cccaacactt ggcaaaggtt
     3601 ggtcaggaat tgacccttct agcccagaaa atcttggagg cagagtcagg taagaaggta
     3661 cagggaaatc ttttagacaa ggggaaaatt acggcaagtg aggatggggt gcttcatctt
     3721 aatcctgaga ccagtgattc tagcatggtt gcagaaggtg ccctactagc ccaactttat
     3781 ccatctttgg aaagagaagg gaaagccaaa ctcacagcct atctaagttc aaaagatgta
     3841 gcaagaatca aggtcggtga ttctgttcgc tatactacga ctcatgatgc cgggaatcaa
     3901 ctttttctag attctactat tacaagtatt gatgcgacag ctactaagac tgagaaaggg
     3961 aatttcttta aaatcgaggc ggagattaat ctaacttcgg agcaggctga aaaacttagg
     4021 tacggggtgg aaggccgtct acagatgatt acgggcaaga aaagttatct acgttattat
     4081 ttggatcaat ttttgaacaa agagtaatgt tcgtgttttt agagttaaat aatttttaaa
     4141 ctgtgagaaa gattcttctt gcagtttttt ctttacaatt tttgaaaaac atctgctatt
     4201 tattcggtta aattcttgtg ttttttggtt ttttgtggta aaatgtgctc aagtaatacg
     4261 aaaggcgaac tttaaaatgt caaaacaatt gatctattcg ggaaaagcta aagatatcta
     4321 tacaactgag gatgaaaatc ttattatttc aacttacaag gaccaggcga ctgctttcaa
     4381 cggtgtcaag aaggagcaga ttgcaggtaa gggagtcttg aataatcaga tctcatcttt
     4441 tatttttgag aaattaaatg cggctggtgt ggcgactcac tttgtggaga aactttcaga
     4501 cacggaacaa ctcaataaaa aggttaagat tattcctttg gaagtcgtgc tccgcaacta
     4561 tactgctggt tccttttcaa aacgttttgg tgtggatgag ggaatcgcct tggagactcc
     4621 gattgtcgaa ttttactaca aaaatgatga tttggatgat ccatttatca atgatgagca
     4681 tgtgaaattc ctacagattg cggatgacca gcagattgcc tacttgaagg aagaaacgcg
     4741 tcgtatcaat gaactattga aagtctggtt tgctgagatt gggcttaaat tgattgactt
     4801 taagctagag ttcggttttg acaaggatgg caagattatc ttggcagacg aattttcacc
     4861 agataactgc cgcttgtggg atgctgatgg caaccacatg gataaggatg ttttccgtag
     4921 aggattggga gaactaaccg acgtttatga gattgtttgg gaaaagttgc aggaattgaa
     4981 ataatctgtt tgcaatggaa aaccttcgtc tctcaactaa aaggactcag gctgaaaagg
     5041 tcccccagac cttttcactc tgtagagaac taggtgaacc aaccgatgtt tacgaaattg
     5101 tctgggaaaa gttgcagggt ttaaaataac aacctcaagg ctgtttggga atattgcaag
     5161 agctgaaata aaggaataag aattgatgga taaacgtatt tttgttgaaa aaaaggctga
     5221 ttttcaggtc aagtcagaga gtttggttag agagctccag cacaacttgg gactgtcaag
     5281 cttgaaaagt attcgtattg tgcaagtata tgatgtattt gacttggctg aggacttgtt
     5341 tgcacctgca gagaagcaca ttttctctga gcaggtaacc gaccatgttt tagatgaagt
     5401 atctgtgcag gcggatcttg ctaactatgc tttctttgcc attgaaagtc tgccagggca
     5461 gtttgaccag cgtgcagctt cgtcacagga agccttgctt ttgttgggaa gttcgagtga
     5521 cgtgacagtc aacacagccc aactttactt ggtgaataaa gatattgatg cgactgagtt
     5581 ggaagctgtc aaaaactacc tgctcaatcc agttgattct cgtttcaagg atatcacgac
     5641 agggattgcc aagcaggagt tttcagagtc agacaagacc attccaaaat tgactttctt
     5701 tgaaagctat acagcagaag actttgctcg ctacaaggcc gaacaaggga tggccatgga
     5761 agtggatgat ttgctcttta tccaagacta ctttaagtca atcgggcgcg tgccaactga
     5821 gactgaactt aaggttttgg acacttactg gtctgaccac tgccgtcata cgacttttga
     5881 gacagagttg aaacacatcg acttttcagc ttctaaattt caaaagcaat tgcagtcaac
     5941 ctatgacaag tatattgcca tgcgcgagga attaggtcgg tctgaaaaac cacaaacctt
     6001 gatggatatg gcgactattt tcggtcgtta tgagcgtgct aatggacgat tggatgatat
     6061 ggaagtctct gacgaaatca atgcctgctc agttgaaatt gaagtggacg ttgatggtgt
     6121 caaggaacct tggctcctca tgtttaaaaa cgaaacccac aaccatccaa cagaaattga
     6181 gccatttggt ggagcggcta cctgtattgg tggagctatt cgtgatccgt tgtcaggccg
     6241 ttcctatgtt taccaagcca tgcgtatttc aggtgctggt gatattacag caccgatttc
     6301 ggaaactcgc gctgggaaat tgccacaaca agtcatttct aaaacagcag ctcatggtta
     6361 ttcttcatat ggtaaccaga ttgggcttgc aacaacctac gttcgtgaat acttccaccc
     6421 aggctttgta gctaaacgta tggaacttgg tgccgttgtt ggtgcggctc ccaagggcaa
     6481 tgttgtccgt gaaaaacctg aagcaggtga tgtgatcatc cttctcggag gcaaaacagg
     6541 tcgtgatggt gtcggtggtg cgacgggctc ttctaaggtt caaacagttg agtctgtaga
     6601 gactgctggt gctgaggttc aaaaaggaaa tgccatcgaa gaacgcaaga ttcagcgcct
     6661 cttccgtaat ggcaatgtca ctcgtctgat caagaagtcc aatgactttg gggcaggcgg
     6721 tgtctgtgtg gctatcggtg aattggcaga cggtcttgaa atcgacctca acaaggtgcc
     6781 tcttaaatac cagggcttga atggtacaga aattgccatc tctgaatcac aagaacggat
     6841 ggcggtcgtg gttcgtcctg aagatgtgga taccttcgtt gccgaatgta acaaagaaaa
     6901 tattgatgct gttgtggtgg cgacagtaac tgaaaaacca aatcttgtca tgcactggaa
     6961 tggtgagaca atcgttgact tggagcgtcg tttccttgac accaatggtg tgcgcgtggt
     7021 tgtcgatgcc aaagttgtgg acaaggatgt caaactccca gaagagcgtc aaacatctgc
     7081 tgaaacactg gaatcagata cccttacggt tctatctgac ctcaaccatg caagtcaaaa
     7141 aggattacag actatctttg actgctctgt tggacgctca acggttaatc acccacttgg
     7201 tggtcgttac caactcacac caactgaggc atctgtgcag aaattgccag ttcaacacgg
     7261 tgtgactcat actgcgtcgg tcattgctca aggtttcaac ccatatgtag ctgaatggtc
     7321 tccataccac ggtgctgctt atgcggttat cgaagcaact gctcgtttgg tggctgctgg
     7381 tgccaactgg tccaaggctc gtttctctta ccaagagtat ttcgagcgga tggacaaaca
     7441 agcagagcgt ttcggtcagc cagtagccgc ccttctaggt tctattgaag cacaaattca
     7501 gcttggcttg ccatctatcg gtggtaagga ctccatgtct ggtacctttg aagaattgac
     7561 cgttccgcca accttggctg cctttggggt gacgacggca gatagccgta aggtgctctc
     7621 tccagaattt aaagctgttg gggaaaatat ctactacatc ccaggtcaag ccctctctgc
     7681 agagattgat tttgacttga ttaagaaaaa ttttactcag tttgaagcca tccaagctga
     7741 ccataaagtg acatctgcat cagctgtcaa atacggtggt gtagttgaaa gtttggctct
     7801 tgctaccttt ggaaactata ttggtgcaga ggtgaccttg cctgaactta aaacagcttt
     7861 gacagctcaa ttaggcggct ttgtcttcac atctcctgaa gaaattgctg gagtagagaa
     7921 ggttggacaa acgaaagcag actttacact gactgtcaac cgtgtgaaac tagatggaca
     7981 caagcttgac agtgcatttc aagggacatt ggaagaagtt tacccaacag aatttacccg
     8041 agcgaaagaa ctagaagaag taccagctgt ggcatcagat gttgtgatta aagccaaaga
     8101 aaaggttgaa aaacctgtgg tttacatccc agtctttcca ggaaccaact cagaatatga
     8161 ttcagctaag gccttcgaaa aagaaggtgc agaggtcaat ttggtgccat tcgtgacctt
     8221 gaatgaagaa gctattgtca agtcagttga aactatggtt gacaatatcg acaagactaa
     8281 tattctcttc tttgctggtg gattctcggc tgcggatgaa ccagatggtt cagctaagtt
     8341 tatcgtcaat atcctgctta atgaaaaagt gcgtgtggct attgatagct ttatcgcccg
     8401 tggtggtttg attatcggta tttgtaatgg attccaagcc ttagtcaaat cgggtctcct
     8461 accctacgga aactttgaag ctgctaacag tactagccca accctcttct acaatgatgc
     8521 caaccaacac gtggccaaga tggtggaaac tcgtattgcc aataccaact caccatggtt
     8581 ggctggtgtg caagtgggcg atatccacgc tattcctgtt tcgcacggtg aagggaagtt
     8641 tgtcgtgacg gctgaggaat ttgcagagct ccgtgacaat ggacaaattt tcagccaata
     8701 cgttgacttt aacggtaaac caagtatgga ttctaagtac aatccgaatg gttctgtcca
     8761 tgccatcgaa ggaattacca gcaagaatgg tcaaatcatc ggtaagatgg gccactcaga
     8821 acgttatgag gatggtcttt tccaaaatat cccaggcaat aaagaccaac acctgttcgc
     8881 atcagcggtt aaacatttca ctggaaaata agacttacag attttctaat agatagtatc
     8941 agtaatgtaa aagtcatgta aatctagctc ttgatgatta caaatgaaaa ttaggtataa
     9001 aaaatgacat acgaagtaaa atctcttaat gaagaatgtg gtgttttcgg tatttgggga
     9061 catccagatg ctgctaagtt gacctatttt ggactccaca gtcttcaaca ccgtggtcag
     9121 gagggggcag gaatcctctc caatgatcaa ggacaactga agcgccatcg tgacatgggg
     9181 cttttatcag aagttttcag aaatccagcc aatttggata aattgacagg agctggtgcg
     9241 attgggcatg tgcgttatgc gactgctggc gaagcttctg ttgataacat ccaacccttc
     9301 ctcttccgtt ttcacgatat gcagtttggt ttggctcata atggaaatct gaccaatgca
     9361 gcctctctca agaaagaact ggaacaaaga ggagcaattt tcagcgcgac ttcggactct
     9421 gaaatcttgg ctcacctcat tcgtcgcagt cataatccta gcctgatggg caaaatcaag
     9481 gaagcgctca gtcttgtcaa aggtggtttt gcctatatct tgctgtttga ggacaagttg
     9541 attgcggctc ttgaccctaa tggctttcgt ccgctttcta tcgggaaaat ggccaacgga
     9601 gcggtggttg tttcctctga aacctgtgct tttgaggtca ttggtgccga gtggatttgt
     9661 gatttgaagc caggtgagat tgtgatcatt gatgacgagg gcattcagta tgacagctat
     9721 acagatgata cccagttggc gatttgttct atggagtata tctactttgc ccgccctgat
     9781 tctaatatcc acggtgtcaa tgtccatacg gcacgtaaga gaatgggagc gcaattggcg
     9841 cgagaattta agcatgaggc agatattgta gttggtgtgc ccaattcttc cctaagcgcg
     9901 gctatgggat ttgcggaaga atcaggctta ccaaatgaaa tgggtctgat caaaaaccaa
     9961 tacacccagc gaacttttat ccaaccgact caagaattgc gggagcaagg agtgcggatg
    10021 aaactgtctg ctgtttcggg tgttgtcaaa ggcaaacgtg tggtcatggt ggatgattcc
    10081 attgtacgtg gaacaacctc tcgtcgtatc gttcagctct tgaaagaagc gggtgcgact
    10141 gaggttcacg ttgccattgg aagtcctgca ctagcgtatc catgtttcta cgggattgat
    10201 atccagaccc gtcaggagct gattgcagcc aatcatacgg tcgaagaaac tcgccaaatc
    10261 attggtgcgg acagtctgac ttatctttca attgatggct tgattgagtc gattggtatc
    10321 gaaacagatg cgccgaacgg tggtctctgt gtcgcttact ttgacggtga ctacccaacg
    10381 cctctctatg actacgaaga agactatcgt agaagtttgg aagaaaagac cagtttttac
    10441 aagtaggcga cagattctcc attaaagaaa aggaaaaaac aaatgacaaa taaaaatgca
    10501 tatgcccagt cgggtgtgga tgttgaagcg ggttatgaag ttgttgaacg gattaaaaag
    10561 cacgtggccc gtacggagcg tgcaggtgtc atgggagctc ttggtggctt tggtggtatg
    10621 tttgaccttt ccaagactgg ggttaaagaa cccgtcttga tttcagggac tgacggtgtc
    10681 ggaaccaagc tcatgttggc tatcaagtac gacaagcacg ataccatcgg gcaggactgt
    10741 gtggccatgt gtgtcaacga catcattgct gcaggtgcgg aacccctcta ttttctcgac
    10801 tacgtagcga cagggaagaa tgaaccagct aagctagaac aagtggttgc tggtgtggca
    10861 gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg aaacggctga aatgccgggc
    10921 atgtacggcg aagacgacta tgacttggct ggttttgcgg tcggtgtggc tgaaaaatct
    10981 caaatcattg acggttcaaa ggtggtagag ggagatgttc ttctcggact tgcttcaagt
    11041 gggattcact caaatggtta ctctttggtt cgtcgtgtct ttgcggatta cacaggtgag
    11101 gaagtcctac cagaattgga aggcaagaaa cttaaggaag ttctacttga gccgactcgt
    11161 atctatgtca aggctgtctt gccgctcatc aaagaagagt tggtcaacgg cattgcccac
    11221 atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt ttgcagatga cctagctgct
    11281 gaaattgatg aaagcaaagt tccagtgctt ccaattttca aagcccttga aaaatatggt
    11341 cagattaaac acgaagaaat gtttgaaatc ttcaatatgg gtgtgggact tatgttggcg
    11401 gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg atgaagcagt ctatgaaatt
    11461 ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca aatgaaaaaa atagcggttt
    11521 ttgcctctgg taatggctca aattttcagg tgattgccga agaatttcca gtggagtttg
    11581 tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc aaagcagctc ggcgttctgt
    11641 cctatgcttt tgaactcaag gagtttgaga gcaaggcaga ctacgaagca gcccttgtcg
    11701 aactcttgga agaacaccag attgacttgg tttgcctagc aggctacatg aaaatcgttg
    11761 gaccaacctt attgtcggct tatgaaggtc ggattgtcaa cattcatcca gcctacttgc
    11821 cagaatttcc aggagctcat gggattgagg atgcttggaa tgctggcgtg ggtcagtctg
    11881 gtgtgaccat tcactgggtg gattcgggtg tggatacagg ccaggtcatc aaacaggttc
    11941 gtgtgccacg actagctgat gataccattg acagatttga agctcgcatc catgaagcag
    12001 agtacaggtt gtatccggaa gtagtgaagg ctctatttac agattgactt tttgatgatt
    12061 catatgatat ctttgatttt aaattggagt cagtgtttgt tgaagacggc ttcaaacgga
    12121 ggtatttgta atgttagaat ctaaaaaaac aactcgatat gtattttatg tctatctgat
    12181 gttattaact tggggaatct tatttaagtt tgaaacaaat cctgaattta tagcattttt
    12241 cttagctcca aggtatatca attggattcc attttcagaa ccactaatag tcgatggaaa
    12301 aattgttttt gctgaaatgt tatttaatct gattttcttt attccattag gtgtttgttt
    12361 ccctttgata aaaactaatt tatctagttt aagaatagtc gggacaggtt tcttgattag
    12421 tttattgttt gagtgcttac agtatatttt agcaataggt ataacagata taacgaattt
    12481 gactttaaat acgctaggtg tctgtgtagg cttactgatt tatcaaattt ttataagagt
    12541 gttcaaatca cagactagaa aatggatcaa tatcttaggt atgcttagcc ttggttttgc
    12601 ttatcttgtt ttactgttac tgcatttaat tggtgtttaa ctaatgatta aaaaggagaa
    12661 tataatgact aaacgcgtct taatcagcgt ctcagacaaa gcgggcattg ttgaatttgc
    12721 ccaagaactc aaaaaacttg gttgggagat tatctcaaca ggtggaacta aggttgccct
    12781 tgataatgct ggggtggata ccattgctat cgatgatgtg actggtttcc cagaaatgat
    12841 ggacggtcgt gtgaagaccc tccacccaaa tatccacgga gggcttctcg ctcgtcgtga
    12901 cttggatagc cacttggaag cggctaagga caacaagatt gagctcattg accttgtggt
    12961 ggtcaacctt tacccattta aggaaactat ccttaaacca gatgtgactt atgctgatgc
    13021 agttgaaaat atcgatattg gtgggccatc tatgcttcgt tcagcagcga aaaatcatgc
    13081 cagtgttaca gttgtggtag atcctgctga ctacgctgtg gttttggatg aattggcagc
    13141 aaacggcgaa acctcttatg aaactcgcca acgtttagca gccaaagtat ttcgtcacac
    13201 agcggcttat gacgccttga ttgcagaata cttcacagct caagtgggtg aaagcaagcc
    13261 tgaaaaactc actttgactt atgacctcaa gcaaccaatg cgttacggtg agaatcctca
    13321 acaagacgcg gacttttacc agaaagcttt gcctacagac tactccattg cttcagccaa
    13381 acagctcaac gggaaagaat tgtcatttaa taatatccgt gatgcagatg ctgctatccg
    13441 tatcatccgt gacttcaaag atagtccaac cgttgtggct ctcaaacaca tgaatccatg
    13501 tggaattggt caagctgatg acatcgagac tgcttgggac tacgcttatg agtctgaccc
    13561 agtgtctatc tttggtggga ttgtcgtcct caaccgtgag gtggatgctg cgacagctga
    13621 gaagatgcac ggcgttttcc tcgaaatcat cattgcacca agctatacgg atgaagcgct
    13681 agccattttg atcaataaaa agaaaaactt gcgtatcctt gccttgccat ttaatgctca
    13741 agaggctagc gaagtggaag cagaatacac aggtgtagtc ggtggacttc tcgtgcaaaa
    13801 tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg gtgactaaac gtcagccaac
    13861 tgagacagaa gcgactgctc ttgagttcgc ttggaaggct atcaagtacg tcaaatcaaa
    13921 tggtattatc gtgaccaacg accacatgac acttggtgtt ggtccaggtc aaaccaaccg
    13981 tgtggcttct gttcgccttg ccattgacca agccaaagat cgtctggacg gggcggtcct
    14041 tgcttcagat gccttcttcc catttgcgga taacgtggaa gaaatcgcca aagcaggaat
    14101 taaggccatc atccagcccg gtggctctgt ccgtgaccaa gaatccatcg aagcagcgga
    14161 taaatacggc ttgactatgg tctttacagg tgtgagacat tttagacatt aagaagataa
    14221 aagggaagaa aacagtttct ttcctttttt ggcttaaaat gctaactgaa acaagattaa
    14281 aacgaacttt tttgatataa tgttggtaaa taattcgcaa aagaggttga ggaatgaaac
    14341 tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc taaaaagtta cttgaatcaa
    14401 aagacgtgga aaaagtcttt gtagctcctg ggaatgatgg gatgactctg gatggtttgg
    14461 aattggtaaa tatctctatt tccgaacatt ctaaattgat tgacttcgca aagaccaatg
    14521 atgttgcttg gacctttatc ggtccagatg atgcccttgc tgctggcatc gtggatgatt
    14581 ttaaccaagc tggacttaag gcctttggtc cgactagggc tgcagcggag ctggagtggt
    14641 ccaaggattt cgccaaggaa atcatggtca aatacggcgt tccgacagca atatatggca
    14701 cattttcaga tttcgaggaa gccaaggcct atatcgaaaa gcatggtgcg cctatcgtag
    14761 tcaaggcgga tggcttggca cttgggaagg gtgtcgtcgt tgctgagacg gttgagcaag
    14821 cggtcgaagc cgctcatgag atgcttttgg acaataaatt tggtgactca ggtgcgcgcg
    14881 tggttattga ggaattcctt gaaggagagg aattttcact ctttgccttt gtcaatggtg
    14941 ataagttcta catcatgcca acggctcagg accacaaacg tgcctatgat ggcgacaaag
    15001 ggcctaacac gggtggtatg ggtgcctatg cgccagttcc acacttacca cagagtgtag
    15061 ttgatacagc ggttgacacc attgtcaagc cagttctaga aggggtgatt aaagaaggtc
    15121 gtccttatct gggagttctt tacgcagggc ttatcctgac agctgatgga ccgaaggtca
    15181 tcgagttcaa cgctcggttc ggagatccag aaactcagct tatcttgcct cgcttaacct
    15241 ctgactttgc tcaaaatatc acagatatcc tggatagcaa ggagccaaat atcacgtgga
    15301 cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa gggctacccg ctagattatt
    15361 caaagggtgt tgagttgcca gtcaaaaccg atggcgacat cattacctac tatgcagggg
    15421 ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg cggacgagtt tatatgctcg
    15481 ttaccacagc agataccgtc aaagaagccc aagccagcat ctaccaagaa ctatcccaac
    15541 aaaaaataga aggactcttc taccgaacag atatcggaag caaggcaatt aagtaaagat
    15601 ataagaataa cgcgccgtag tcgccaaaca cgataatggt cgtcgtggtg aaaagaccag
    15661 aacagtgaat gttctggtca gggggaaact tggagaccgt aggctcaaag tttaggaatg
    15721 aaaccgaagg tttgcttccg cctccatcac ctaagaccat tatcaaaaag aaaaataaaa
    15781 attcacaaaa tacgttaatg gtcgtatggt ttgcgagcgt tagcgagcta atatagaata
    15841 atcaccgccg ttgtgaaaga acgattggat gacaatccaa tcgttcaggg aaattggaag
    15901 accttgggtt tccaatttag gcatgagaca cctttggtgg ctgctgccgt ccctcacaag
    15961 ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat gaaaccagta atttccatca
    16021 tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac agcagaagtc ctagaccgct
    16081 tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg tacaccagac ctcatgttca
    16141 aacatgcaga agaagcccgt agtcgtggca tcaagatcat catcgcaggt gctggtggcg
    16201 cagcgcattt gccaggcatg gtagctgcca aaacaaccct tccagtcatt ggtgtgccag
    16261 tcaagtctcg tgctcttagt ggagtggatt cactctattc tatcgttcag atgccgggtg
    16321 gggtgcctgt tgcgaccatg gctatcggtg aagctggagc gactaacgca gctctctttg
    16381 ccctccgtct cctctctgta gaagataagt ccattgcgga tgcacttgcc aactttgctg
    16441 aagaacaagg aaaaatcgca gaggagtcgt caaatgagct catctaaaac aatcggaatt
    16501 atcggtggcg gtcaactggg tcagatgatg gccatttctg ctatctacat gggccacaag
    16561 gttatcgcgc tggatcctgc ggcggattgc ccggcctctc gcgtggcgga aatcattgtg
    16621 gcaccttata acgatgtgga cgccctccgt cagttggcag accgttgcga tgtcctcact
    16681 tatgagtttg aaaatgtcga cgctgacggt ttggatgccg ttatcaagga tggacaactc
    16741 cctcaaggaa cagatctgct ccgcatttcg caaaatcgta tttttgaaaa ggactttttg
    16801 tcaaacaagg ctcaagtcac tgtggcaccc tacaaggtcg tgacttctag cctagacttg
    16861 gcagatatcg acttgtcgaa aaactatgtc ctcaagactg cgactggtgg ctacgatggt
    16921 catggacaaa aggttattcg ttcagaagca gacttggaag cagcctatgc gctagcagac
    16981 tcagcagact gcgtcttgga agaatttgtc aactttgacc ttgagatttc tgtcatcgtg
    17041 tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg aaaatatcca ccgcaacaat
    17101 atcctgtcta agaccatcgt accagcccgc atttctgaaa gtctagcaga caaggctaaa
    17161 gctatggcag tgcgaatcgc agaacaactc aacttgtctg gaactctctg tgtggaaatg
    17221 tttgcgactg atgatgacgt tattgtcaat gaaatcgccc cacgaccaca taactctggg
    17281 cactattcta ttgaaacctg tgatttctct cagtttgaca cccatattct gggtgttctc
    17341 ggagcaccat taccagtcat caaactccat gcgccagccg ttatgcttaa tgtcctcggt
    17401 cagcatgtcg aggctgctga gcaatatgtg acagaaaatt caagcgccca cctccacatg
    17461 tatggtaaaa tagaagcaaa gcataatcgt aagatgggac atgtgacttt gtttagtgat
    17521 gtgccggata gtgtggaaga gtttggggaa gggattgatt tttaggacaa gtctatgata
    17581 caaattatcg ttaatacatt tattgaaaag gataagactg gagcagttgt tgaagtgttg
    17641 tatgccagtg ctgaccaaga taaggtacaa gctaaatatg aagaactagc tgcacaatac
    17701 cccgaaaatt atttagctat ctatgatgta ccgctggata cggatttgaa tacactagat
    17761 cattacccgt ctgtgtttat tgaaaaagag gagtttgagt agaaatcttg gtttacctag
    17821 atagcttatt cccaacagct taagaagaaa ggaaaaatta acacatgatc aaccgttact
    17881 ctcgccctga gatggcgaat atttggagtg aagaaaataa ataccgtgct tggcttgagg
    17941 tggaaatcct ctctgacgag gcatgggctg agttggggga aatccctaag gaagatgtgg
    18001 ctttgattcg caagaaggcg gactttgaca tcgaccgtat tttggaaatt gagcaggaga
    18061 cgcgccacga tgtggtggct ttcacgcgtg cggtttctga gactcttggt gaagagcgca
    18121 agtgggttca ctatgggtta acttctaccg acgtggtgga tactgcttat ggttacctct
    18181 acaagcaggc caacgacatc atccgtcgtg accttgaaaa cttcactaat atcatcgctg
    18241 acaaggccaa ggagcacaag ttcaccatca tgatggggcg tactcatggt gtgcacgctg
    18301 agccgacaac ctttggtctt aaattggcaa cttggtacag cgaaatgaaa cgcaatatcg
    18361 agcgtttcga gcatgcagct gctggtgtag aagctggtaa gatttctggt gcggttggga
    18421 actttgccaa tatcccacca tttgtagagg agtatgtctg cgataaactt ggcatccgtg
    18481 cccaagaaat ctctacacaa gtccttcctc gtgaccttca cgctgagtac tttgcggttc
    18541 ttgccagcat tgcgacttca atcgaacgta tggcgactga gattcgtggt ctacaaaaat
    18601 ctgagcaacg cgaagtagaa gagttctttg ctaaagggca aaaagggtct tcagcaatgc
    18661 ctcacaaacg caacccaatc ggttctgaaa atatgactgg tctggcgcgt gtcattcgtg
    18721 gtcacatgat tacggcttat gaaaacgtcg ctctctggca cgaacgcgat atttctcact
    18781 catcagctga gcgtatcatc acaccagata cgaccatttt gattgactac atgctcaacc
    18841 gttttggaaa tatcgtcaag aacttgacag tcttcccaga aaatatgatc cgaaacatga
    18901 actcgacttt tggtcttatc tttagccaac gggctatgtt gacattgatt gaaaaaggca
    18961 tgacccgtga gcaagcctat gacttggtgc aaccaaaaac agcctactct tgggacaacc
    19021 aagtagactt taaaccactt cttgaggcag attcagaagt aacatcacgt ctcactcaag
    19081 aagaaatcga tgaaatcttc aacccagttt attacaccaa acgagtggat gatatctttg
    19141 aacgtcttgg actaggtgat taattaaaaa ataaacagcg agcttcaatc tcgctgttta
    19201 ttttttatcg aaaagactta gtcttctttt cttttagtga gtccataggc tgctagtgtg
    19261 gacatgagtc ctgcgactac tagtcctgca gaatcgtgag ttcctgtttc aggaagtttt
    19321 ttctctgtta ccacaggagc tggatcttga ggaagagctt tgctttcctc agcaggagca
    19381 gttgatggag ctggttggct tgggatttct agttttggtt tttcttcagc aatagcggct
    19441 tgtccgtttt catcgcctac atgtgttacc atagttccga cttcgactat ttgagtaacg
    19501 gcttcctgtg ctacgacact atttacaagt gttttcactt ccttaccatc ggcagaagtg
    19561 ctcacagagt agaagttgct acgatgtcca ttgacgccct tagtaatgac ttgtgttttt
    19621 cctttgagta agagtggatt ttcacgagtc actgtggtaa atggaatttc ttctttttgg
    19681 atatccagtc taggttttac ctcagtagtt ggtgcaagac cactttcatc acccttgtga
    19741 gttacaggag cgccaacttc aaccacttgg tttataactt ctttggttac ctggctatca
    19801 aggactgttt ctgttgtttt tccattttca gtgagtacag agatgtaatg agttcgttca
    19861 cctttgactc ctgctgtgat aatattttct tgaccagctg ggaggttagg attttctttc
    19921 ttgataactt caaatggaat ttcttcagtt cttgtgatga gttctggtct ggtttcaaca
    19981 ttggcagcca cttcattttc atctaggctt cctgaatgag ttgcagctgg tttgaggcct
    20041 tgaagagcgg cttttaggtt ggctacaagt gtgtcaagct cagcttgttt attacggttg
    20101 aggttgtaat ttagagctgt tttagctgcg tcaagggcct caagactttc tttactatat
    20161 ccttctaagt ttgtaggaat tttagctaat tcttcgcgga gagcattata attagcacgg
    20221 aagtagtctt tgttgtggtc tgcaaaggca gtcatgagtt caaagatttc ctcttccttg
    20281 tattcagcgc ttggtctatc tgcccagatt gaaagcatac ttccgactgt tggaagatct
    20341 acttcaggat atttggtaga agctagttga ttgaatggaa ctttaccaga attttcaact
    20401 gcttttgaaa gagggtaagc ttcctcttgt ttgtgattgc ctataacata ataccagtca
    20461 ccgttggtat tcaagaattt atagcctttg cttgctaggt attgaggtga tgcgaggtta
    20521 tatccccacc agcctttaga ccagtaagaa atcaagacat ctttgtcaaa ctgaacatcg
    20581 tccttgtctt catagtagaa gccatcgttg aaggccattg gttgaagccc tctttctttg
    20641 gccatagctg cgagggtgtt ggcatattcg gcaaatttgc catagagttg ataccacttg
    20701 aggtagtacc agccttgggc actagtcgca tcgttggcgt attcgtcagt accaaagttg
    20761 aaaatctttg ttttacctgc aaagaagtcc atgtatttac cgatgagggc ttttacaaag
    20821 tccatcgctt cttcgttttt caagtccata gttgtttttg aaactttatc aaagtgggct
    20881 tgaggatttt taatacctaa tttttccatg gcaaccagca tagcatccat gtgacctgga
    20941 ctgttaatag ctgggatgag accgatgtcc ttagatttag cgtattcaat tagctctgtc
    21001 acttctgcct gtgttagtgc agtaccgttt ggatcgtcgt agtaagcttt agttccttcg
    21061 ataatagctt ttttaacgtc atcactagca taggtttttc cgttggcagt aatggtcata
    21121 tcattgagta gaaagcgaag tccgtcattt cctagaagga gatggacatc agaatatccg
    21181 agctcactgg ccttgtctac gatgcgtttg agctggttca gagtaaagta tttgcgtcca
    21241 gcatcgattg agattacctt gtttttggca agtttttcaa cctcacgttt agcttcttct
    21301 tctttttgag cttcaggcgt gagggtcaag ttgttgacag tttcttgaag tttagcaatg
    21361 gcttgatcaa tcgtatcttg ttgggcacgg ctaaggttgc tatcgagaga gcgaatagct
    21421 ttttcagctt cttttacggc cgcgacgctt tctgcagtat aacggttcag gtcttttggt
    21481 acctcgttaa gtgcttgctc tgcagattca taatcagctg cgaagtattc agcgttggca
    21541 tttgcaaaat gacgcatgag tttgaagagg cgtgatggtg aataacgtgc agatggagtg
    21601 tcagcccaag cagctaccat accaccgatg attgggatat cagctccttc tgtttttggt
    21661 acagaagtga ttggtgtgtt tttaatacca ttgagcccct gatcgagatt gtaccagcct
    21721 tggccatcag cgtttcgtcc aagaacgtag taccaagcat cattggtatt aaggatttgg
    21781 tgaccttttt cagctagtag tttagaagaa gcgacatcgt agcctcccca accaccagtc
    21841 cacatagaaa cgatgatgtc tttgtcaaaa ctaccaaagc ttgtgtcgct attgtagtag
    21901 ataccgtcgt taaaggccat tggtttgaga ccgtgcgatt ttacaatacg agcgaggtca
    21961 ttggcgtagg caataaattt ttcatagcct tttacagggt agccttcgtt tggatagtat
    22021 ttatcagctt gaagcacact ccaaccttta gcatctgtcg catcattggc atattcatca
    22081 agtccgatgt tgaagatttc agtctttttc gcgaaataag cagcatactt gtcgataagg
    22141 gcttttgtaa aagcgacagc ttgttcgttg tcaagatcga cagtacgggc tgatttcttc
    22201 ccaaaatagc taaagttagg gttttggatt cccaattctt tcatggcatt gagaatcgca
    22261 tccatgtgtc caggactatt tactgtcgga atgagaccga tacctttatc tttggcatag
    22321 ttaatcagat ctgtcatttg actttctgtt aagtgattgc cgtttggatc gttgtaataa
    22381 tcatttgtac ctttttcaat ggcgcgtttg acatcgtcac tggcataggt cttgccgtta
    22441 gctgtgatgc tcatatcgtc caacatgaaa cggagtccat catttccgac taataggtgt
    22501 aaatcagtgt agccataatg tttcgcttta tcgatgattt ccttgagctg ttctggtgag
    22561 aaatatttac gtccagcatc aatagaaaca attttctttt tcgctagttt ttcatttaca
    22621 gttgcagcac gttcctttcc tgcctctgtt tccggtttgt cagcctctgc tttcgcttca
    22681 tcttttttag ctggtttatc cttgtcagtc ttgtctgtat ttgactcttt agaatcaacc
    22741 tctttcgctt cttccttttt agggctagct tcttctgcct ttttattagc agtttctttt
    22801 tcagcagaag ttggagttac cacctctgct ttatcactag gagttgaact aacttcctct
    22861 tgtggttttt cttctgtttt tggaagacta gctaccttat cagtagctgg agtttctgtt
    22921 tctacagttt ttggagcttc tggttgaagc tctgctttag gtgtttcctc agtccgattt
    22981 tcggatgatt gaggggaatc agaaactgta tggatggtcg gttggttttc tgtagtagta
    23041 gtagtaactc catcggctgc aacagtctgt gcttggaagg caaatccaat tagaacagaa
    23101 gctgctccta cagcgtattt acgaatagaa aaacgctgtt gtttttcatg tttcattgca
    23161 aaacctcctg attgcattgt tatattgata gcgattatat aaatcaacgc ccttatttta
    23221 tttcttatat taacgagagt caagaggaga tgacaaaaaa ctataataag tataaaaaaa
    23281 tataaaattt aaacttaaga tttcagattg gtcggaaaaa atgcatatat atatctaata
    23341 taatttttgg ttctatttct ataaaatatt ccacaaatta tagaatcttc caaaaatagg
    23401 taagcgctac ctttttggtg tggtataata agcatagaaa aagcccgagc gattaggctc
    23461 aggctttctt cttagtgatc acggtcacat gagataaatt taatcttgta gtaatcagat
    23521 cgtttgtaag tttcactgta ttctaaaact tggccagttg attcgagttt ggtgatttta
    23581 gtttgtagga cagtagggaa ttgttcatcg actccgagga ctgaagccgc atgttctgga
    23641 gttggaaaga ctatttcgtt gatttcttca aagtgttcat cattcatgtg aatgtggtag
    23701 tctaacttga aacgattata gatagaacta tagtattcaa ggtttggata atttgcgttg
    23761 atatattgtt ctgggatgta ggatgtatgg tagatataaa cgacaccgtt tgattcgcgg
    23821 atacgttcaa tcttgtagta gaattgatcg ccgcgtagac ccaatttttc caagtaaaca
    23881 agcttgtttc cgcgttcaat tgaaagaaca gttaccttat catctttagc attgaagagt
    23941 tcaatatctg aaaactctac aagcttgtgt ttgcgtgcac gtgaaacgaa ggttcctttt
    24001 ccttgttggc ggacaatata gccatctttg gcaaggtcgt ttaaggcgcg aacaactgtg
    24061 atagagctga catcgtacat tgaaatgagt tctgcttcag tgtaaaattt atctccactg
    24121 ctaaactgcc cagagatgat tttatttttt aattcgtc
//
LOCUS       DAVLTM010000018        23545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLTM010000018 DAVLTM010000000
VERSION     DAVLTM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.018"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..635)
                     /locus_tag="VYN16_000637"
     CDS             complement(<1..635)
                     /locus_tag="VYN16_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403208.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQDL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(637..1464)
                     /locus_tag="VYN16_000638"
     CDS             complement(637..1464)
                     /locus_tag="VYN16_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1403209.1"
                     /translation="MMIKIIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1486..1821)
                     /locus_tag="VYN16_000639"
     CDS             complement(1486..1821)
                     /locus_tag="VYN16_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV1403210.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(1822..2538)
                     /locus_tag="VYN16_000640"
     CDS             complement(1822..2538)
                     /locus_tag="VYN16_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1403211.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEQRDQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLLD
                     LNEDKMKAEVERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            3051..3662
                     /locus_tag="VYN16_000641"
     CDS             3051..3662
                     /locus_tag="VYN16_000641"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1403212.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3707..4435
                     /gene="rlmB"
                     /locus_tag="VYN16_000642"
     CDS             3707..4435
                     /gene="rlmB"
                     /locus_tag="VYN16_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1403213.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKNEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4474..5385)
                     /gene="trxB"
                     /locus_tag="VYN16_000643"
     CDS             complement(4474..5385)
                     /gene="trxB"
                     /locus_tag="VYN16_000643"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1403214.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLGDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5451..5675)
                     /locus_tag="VYN16_000644"
     CDS             complement(5451..5675)
                     /locus_tag="VYN16_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1403215.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5753..6496)
                     /locus_tag="VYN16_000645"
     CDS             complement(5753..6496)
                     /locus_tag="VYN16_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1403216.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6496..7296)
                     /locus_tag="VYN16_000646"
     CDS             complement(6496..7296)
                     /locus_tag="VYN16_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1403217.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7454..7810)
                     /locus_tag="VYN16_000647"
     CDS             complement(7454..7810)
                     /locus_tag="VYN16_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1403218.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7804..8334)
                     /locus_tag="VYN16_000648"
     CDS             complement(7804..8334)
                     /locus_tag="VYN16_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1403219.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(8334..8828)
                     /locus_tag="VYN16_000649"
     CDS             complement(8334..8828)
                     /locus_tag="VYN16_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403220.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            8963..9409
                     /locus_tag="VYN16_000650"
     CDS             8963..9409
                     /locus_tag="VYN16_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1403221.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9406..10053
                     /locus_tag="VYN16_000651"
     CDS             9406..10053
                     /locus_tag="VYN16_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1403222.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10074..10655)
                     /gene="pdxT"
                     /locus_tag="VYN16_000652"
     CDS             complement(10074..10655)
                     /gene="pdxT"
                     /locus_tag="VYN16_000652"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1403223.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10656..11531)
                     /gene="pdxS"
                     /locus_tag="VYN16_000653"
     CDS             complement(10656..11531)
                     /gene="pdxS"
                     /locus_tag="VYN16_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1403224.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFCNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11683..13062)
                     /locus_tag="VYN16_000654"
     CDS             complement(11683..13062)
                     /locus_tag="VYN16_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1403225.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAER"
     gene            13356..14279
                     /locus_tag="VYN16_000655"
     CDS             13356..14279
                     /locus_tag="VYN16_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1403226.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14339..14944
                     /locus_tag="VYN16_000656"
     CDS             14339..14944
                     /locus_tag="VYN16_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1403227.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEETDGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            14963..16207
                     /locus_tag="VYN16_000657"
     CDS             14963..16207
                     /locus_tag="VYN16_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1403228.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISVSLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGNADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16323..16580)
                     /locus_tag="VYN16_000658"
     CDS             complement(16323..16580)
                     /locus_tag="VYN16_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1403229.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEKGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16622..18658)
                     /locus_tag="VYN16_000659"
     CDS             complement(16622..18658)
                     /locus_tag="VYN16_000659"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1403230.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(18882..19799)
                     /gene="glyQ"
                     /locus_tag="VYN16_000660"
     CDS             complement(18882..19799)
                     /gene="glyQ"
                     /locus_tag="VYN16_000660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1403231.1"
                     /translation="MSKKLTFQEIILTLQRFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(19993..20754)
                     /locus_tag="VYN16_000661"
     CDS             complement(19993..20754)
                     /locus_tag="VYN16_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1403232.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(21027..21869)
                     /locus_tag="VYN16_000662"
     CDS             complement(21027..21869)
                     /locus_tag="VYN16_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1403233.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSITTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(21983..23374)
                     /locus_tag="VYN16_000663"
     CDS             complement(21983..23374)
                     /locus_tag="VYN16_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1403234.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
BASE COUNT         6863 a         5359 c         4192 g         7131 t
ORIGIN      
        1 tagtaaagat agtctttcaa ggcttttgac aaagcctgat aaccagcaat actgaggtgc
       61 agtccatcag ttgtatattc ttttttgagt tggcctgctt ggtctgtcaa acaatcaaat
      121 actggcacaa attccacctg catataggca gatgcaagat cttgataggc ttgattccag
      181 ttctgaattt tttcattcga gcggatatag actgcctgct gatactcctc tccctcattg
      241 acaggtaaaa tggaaagcaa tttaatctct gtcaatggat aatcgcgagc aacggattga
      301 atgatagctt cgagattatt gagagcctca ttcacaggaa catcctttcc gatatcattt
      361 gtcccaatca gaagaaaaat tttatctact gctccaccat atagatgagc atcaaggttc
      421 tctagtaaca gtcctgtctg atagccacga attcctcgat tgacaatcat ttttgaagtc
      481 ccaaatagct cctgtagagg ataatactcg acaatggaat ccccaataaa aagaatgttt
      541 ggttctacaa cagaaatgtg atttaggtga cgatacttgg tttgaatttt ttcttgttcc
      601 tttaggagcc aattttctaa taactgtact gccacttcat tctccttttt ctaaccagtt
      661 ttccaagact tgataaactc ctccttggct attggctggt gctagagcag tcgccacagc
      721 tttggctttc tcatcagcat tttccatcgc ataggcaatt ccagccattt caagcatttc
      781 aacatcattt tcactatcac caaaagccat gatttcttgg gatttcaagt cccagcgctt
      841 gagtaattcc tccaagcccc atgctttatg aatcccagct tggaggatat caatgcaacc
      901 atagccactg gatacagctc ggacacggcc atcaaagaga gcattgattt cttccaaaac
      961 cgaactcaaa cgttcctcac caacaaccat gctcatcttg agcacaccac caaagaggtc
     1021 agatgttaat tcatccacaa attgcatccg ttggtagaat ttttcaatca tttctggagt
     1081 cataaaactt tcaagatctg taaaaatcgt accttccttg acaaaatcac ccttcatccc
     1141 cgttacaaca aactggtcct gacacgctcg acccttgaaa tgagccaaag ccttgttgac
     1201 aatggcgtca tcccatgtct gagcctgaat caattcattg ttttcaaaaa tacgagcgcc
     1261 attagcaaca accagaacca ctcgatccac caagggactc agtagttgtc tcatgcggtg
     1321 aatttcattg cccgtcgcaa tgacaaaacg aatgcccctt tgatccaact gatctaaaat
     1381 cttttccaat cgtgggagat caagctgacc tctagcatcc agcaaggtcc catccatatc
     1441 tgtcgcaatt atcttaatca tcatttactt tcctctggat tcaagctaat accacttcaa
     1501 tcccacatgt tcattcattt ctttataggc tgtatcaatt cgttccttag tcgcttcatc
     1561 taactggtca cgatgactgc caccctctat aaaatcttcc aaaatataag tttggtaatg
     1621 atacaaggga tatccttctg gtgaaaaggt tccctttggt gttcgattga cccaagtagg
     1681 atgagcttta gctgttccga tagttgtttt tccatccttc ttcttgatgg tgacatccat
     1741 gagaacacca cgttcagtcc acttagcatt ctcttcatct cccatagatt caatccgttg
     1801 attggaaatg aagttcccca tttaatagat aatgagtttc ttatctccat ctttttcaac
     1861 cgtttcagat ggttcaacaa cgtgaggatg ccctccaaag ataatatccg ctccccaatc
     1921 gatcatcttg tgataaagag ctttttgttc ttcagttggt tccaatcgat actcaacacc
     1981 catctgaagc atgataatgg tgatatctgc ttccttctct gcccgttcaa cttcagcctt
     2041 catcttatct tcgtttaaat ctaaaagata acgattatag tcttcctgag aaatatactg
     2101 ctcaattcca ttgaaaccat aggaataggc taacaatgca accttgatac cattcacttc
     2161 cttaatgacc agcggagcct gatcacgttg ttcgtgcgta taaactccga ttggagtgat
     2221 tccagctttc tcaataatat cggccgttga aataactccc tcaatttgcg aatccaaaat
     2281 atgattatga gctaaatcca gcacatgata acctgcttcc ttaatagcat ccataacttc
     2341 agcaggagca ttaaagagaa gataacccgc taaataatga tccttattaa tggttccttc
     2401 gaaatcacca atagctaaat ctgcttgctt gagccaagga gtcacatact caaaattttc
     2461 atgaaagtca taggtaccgt cttctttttt agctgaaaag aaaagtccat cgtggtagag
     2521 caaatccccg tgggccataa ttctggcagt tttttcctct tcctactcct gagacttttg
     2581 cttagtcccc tcttgagaaa tagtatctcg tttttgacta gtcaagggat tccttggaag
     2641 ctttcaaaca acaagaccaa gcccattgat aaaccaactg ctagcaagag tatcgccaca
     2701 aatcccttat tgctccactt gcgataactc ctaaaaagtt taccaagccc ttcataaaac
     2761 gaaaagctaa accaccctga tttcgatttt gtcttctttg tatcttcgtt ctccctactt
     2821 tcttatgcaa gccttttctt tttattatat cacagataag tatttctttc acaattgaat
     2881 tgaacttcct atctattttc tataaatcct aaatgccata atgctttcaa ttcttgtcat
     2941 tttgtgatat catgtagagg aaatgaacta atccacagtg gcttattcca agtataccac
     3001 ttgggctttg gcagtagcta actgcactaa acataatata aggagagaaa atgtctgcaa
     3061 tagaacgtat tacaaaagct gctcacttaa ttgatatgaa cgatattatc cgtgaaggga
     3121 atcctactct acgcgcgatt gctgaggaag tcactttccc cctatctgac caggaaatca
     3181 tcctaggcga aaagatgatg caattcctta aacattccca agatcctgtc atggctgaaa
     3241 aaatgggact ccgcggtggt gttggactgg ctgctcccca gttagatatc tcaaaacgca
     3301 ttatcgctgt tttggtacct aatattgttg aagaaggcga aactccacag gaagcctacg
     3361 atttggaagc cattatgtac aatccaaaaa tcgtctctca ctctgttcaa gatgctgctc
     3421 ttggcgaagg agaaggttgc ctgtctgttg accgtaacgt gcctggctat gttgttcgcc
     3481 atgcccgcgt tactgttgac tactttgaca aagatggaga aaaacaccgt atcaaactca
     3541 aaggctacaa ctccattgtt gttcagcatg aaattgacca cattaacggt atcatgtttt
     3601 acgatcgcat caatgaaaaa gacccatttg cagttaaaga tggtttactg attcttgaat
     3661 aaagaaaatc ccgttgcaag acggggtttt gtgttataat agaggcatga aaacaaatga
     3721 tattgtctat ggcgtccacg ccgttaccga agccctcctt gcaaatacag gaaacaaact
     3781 ctacctccaa gaagatctcc gaggtaagaa tgttgagaaa gtcaaggaac tagctacaga
     3841 aaagaaggtg tccatttctt ggacatcaaa aaaatctctc tctgagatta ctgaaggtgc
     3901 tgttcatcaa ggttttgttc tacgagtgtc tgaatttgcc tatagcgagc tagattacat
     3961 ccttgcaaaa acacgccaag aagaaaatcc acttctattg attctagatg gtctaaccga
     4021 tccccataat ctaggttcta tcttgcgaac agccgatgcg accaatgttt caggtgtcat
     4081 cattcccaag caccgtgctg tcggagtaac tcctgtcgtt gccaaaacag ccacaggtgc
     4141 tattgaacac gttccaattg cccgagtgac caacctcagt caaaccttag ataaacttaa
     4201 gaatgaaggt ttctggacct ttggaacgga tatgaacggt actccttgcc acaagtggaa
     4261 tacaaaaggg aaaatcgccc tcatcattgg aaatgaagga aaaggtatct ctagcaacat
     4321 aaaaaaacag gtcgatgaaa tgattaccat tccgatgaat ggacatgttc aaagccttaa
     4381 tgccagtgtt gctgcggcca ttctcatgta cgaagttttc cgaaatagac tataaaaaag
     4441 tttccagtca tctgattgga aactttttta tgattaacta tgttctgtaa tgaatttata
     4501 ggcttcttga ccagcgatag ctccatctcc aaccgctgtt gttacttggc gaaggtcttt
     4561 caagcgaaca tctccaactg caaagatacc gtcgactgca gttttcatgt ggttatctgt
     4621 cacaatccat cctgcctgat cttggatatt caattcttta acaaaatcgc caagagggtc
     4681 caatccaaca tagataaaga caccaccgaa gacttgttct gtcacttgac ctgttttcac
     4741 attttcaaat acgactgatt ctactcggtt ttcacccttg atttccctta ctacagaatc
     4801 ccagataaag ctgatttttt cattcgcaaa ggcgcgatct tgtaaaacct tttgggcacg
     4861 aagttggtca cgacggtgaa caatggtaac agtcttagca aaacgagtca agaagagggc
     4921 ttcttcaaca gctgaatctc caccaccaac taccaataaa tcttggtcac ggaagaaagc
     4981 accatcacac acagcacagt aagaaacacc acgactgttc agttcttctt ctccaggtac
     5041 tcccaaagga cggtgtttag aaccagttgc tacgataact gtacgtgttt catatgtttg
     5101 gtcatcagtc atcactttct taaaatcacc atggtcttcg acattttcaa cataaccata
     5161 aatgtgctcc acaccaagat tttcaagtgg ttcaaacatc ttttcagcca attcaggtcc
     5221 actaatatta gcgtatcctg ggtaattttc gatatcagat gtattattca tctgaccacc
     5281 tggcagacca ccttcaatca aagctacttt tagattgctt cgagcagcat acaaggccgc
     5341 agtcatccct gcaggtccag caccgataat aatagtatcg tacatataga ttccttcttt
     5401 cttgttgtaa ctatctttat tctaactctt tcttgtcaat cacgcaagga ttatgccttg
     5461 aaaacaagaa ttaaactcat aaccgcaaag gataatactg ggacaaccaa gactccaatc
     5521 ataatcatat catagagatg ggcttggcga gccctggctg tcttatcaac tcccgacatg
     5581 gctctcagac caatccaaat ccctaaaaaa atcagaacaa ggatggtggt caagatcaaa
     5641 ctctcgaaat ataaagaaaa tagttgcagt agcatgattt ctctcatttc tatctttttt
     5701 aaagagtaaa ctcagctagt ccaactaact gagttttcct ttatctatta tatcaaatat
     5761 aagtccgttt gtaactagcg aagaattctt ttgtccgctc ttctttaggg gtgtggataa
     5821 tctcatccgg tgttccagac tcgatgattt tccccttatc taagaagaga atcttatcag
     5881 ccacttgggc tacaaaggac atgtcatgac taaccaaaat catggtttga cctgacttag
     5941 cagcatctgc aatagacttt tctacttcac caaccaattc tgggtcaagg gctgaagttg
     6001 gttcgtctaa gagcaaaaca tctggtttca tagcaagcgc acgcgctagg gcaacccgtt
     6061 gcttctgtcc acctgataaa tggcgaggat aatggttttc acggtccgaa agcccaacct
     6121 tagccaactc ttccttggca atcttagtcg cttcttggtc agataatttc ttgacaacaa
     6181 ccaagccttc tttcacatta tcaagtgctg ttcggcgttc aaacaaatta aactgttgga
     6241 aaaccataga caacttacga cgtagggcaa ggatttcttc ttgagtgatt ttagaaaaat
     6301 caactgaaaa accatcaatc tgaatagagc cactgtcagg tgtttcaaga taattgagac
     6361 tgcgaagaaa ggttgatttt ccagctcctg aagaaccaat caaggctaca acttcccctt
     6421 tttgaatatc caagtccaga tgatccaaga cagtctgtcc tgaaaaggat ttgcttaaat
     6481 ttgaaatctt aatcattaac gaaggtctcc tttcacatct gtttgcactg tatcaggtgc
     6541 agaaatagcc atttttctct cgatgaaacg accgaggctt tcaattccga tattgaccac
     6601 ccaataaaca agggcaacgg agatgaagcg ttcaaaatag cgataatcag ctccacctag
     6661 aatctgagct tgggcaaaga cttccacaac acccgcacta aaagctagag atgttccctt
     6721 ggtcaaaccg atgagggaat taatcaaggt tggagtagct accaccgctg cattaggaat
     6781 aatcactcgt cgataaactt gcgctcgggt catacccaga ctgcgtgccg cctcaatctc
     6841 accaggatta actgagagaa tggctgcacg aatggtttca ctagcataag ctgcctcatt
     6901 aaaggcaaag gcgacaatcg caaaagctgc agctggaatc gcattgatat tgagaccagt
     6961 tccccattgc tgattgaggg ctttcaaagc caaagggatt ccgtagtagg tcaacatgag
     7021 ttgcaccaaa atcggtgtcc cttttaagaa actaacaaag aaggcctgca agggatataa
     7081 aatcttgaca cgattgatct tcacaatggc aaaaagaagc gccaaaacca agccaaaaag
     7141 ggcaccacca attgtcaaca taattgttgt tggaagttgt tggacaattc tagggattcc
     7201 atcaaagacc gaacgtaggc taaacagctt gccatccgga atcaattgca tcaagttttg
     7261 gtaccaatct gatgctaaaa tcgttgtaac attcataaaa acatcctctt ctgataatga
     7321 ttcattctac catgcttctg ccgtcaatga aattatttgc tacggtcaga tacagacttt
     7381 gtctacctac tagtttatca tactttgaaa cagggaagtt atatatttta tctatcaaag
     7441 agatagataa aaactatttc agtcccagtt cttcataaga ttttcgataa ccgatttgct
     7501 taacagttcc attttctact aaaataggcc gttttaacaa cataccgtca cttgctagca
     7561 actcagccgc ttcttggttt gacaaacttc ctaccttatc ttttagccct aattcacggt
     7621 atttgatacc actggtgttg aaaaattgct tcaattcaaa tcctgaggtt tctagccaat
     7681 tcaaaatgac ttcttggcta ggtgtttctt ccacgatatg gacggcttta tagtccacac
     7741 ctaattgatt taattcttgt tttgcttttt tacaagttga acatttgggg tattcgataa
     7801 attctaacat gtgcttctcc ttctattgtt tctatcttta aaatctactc cttcatgctc
     7861 caagagccaa gctttctttt ccactcctgc agcataacct gtcagacgct tgcctgctcc
     7921 caacacacga tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat
     7981 tgcttgagca gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc
     8041 ataaggaatg ccctgtaaat agtcccaaac tctcttttca aaattcgttc cgattggcgc
     8101 caagagcaag ttggataaat cctgaggctt gcctttaaag taatcatcta agcaagcaat
     8161 aactgggtct aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct
     8221 ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag
     8281 tgatagaata ccaattggtg aggagtagag gatttttaca tacttctttt gcattatttc
     8341 tttaattttt gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa
     8401 ccaagttttt caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa
     8461 tcaagataat caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc
     8521 tgcacatctg ctgccacagc aatacggtga aagactagat actctgactc tcccgcttgc
     8581 cagtttccct cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag
     8641 gctagcagtt ctccctcttc caaggctacg taggcttgcc ctgagatgat atcatcgata
     8701 ataacatctg catttggata gccattttgc cactggtcac taccagcatc tgctaaacat
     8761 tttttggcat cctccatcac ctgcatgatg gcatctactt catttggaaa agctaaacga
     8821 atttccatct tttttcctca tttctgacta gtttctccca tcatagcata atttaagtat
     8881 ttctacaagc agttaaaact tgactttgtg tttttgttat tttataatct agttatcaaa
     8941 actatataaa aaggagttga aaatgaaaac taccttttcc tacccaaaat gggcagaaat
     9001 tccaaacatt gacctctatc tggaccaggt tttgctctat gtcaatcagg tctgcgcccc
     9061 tatctctcct aataaagaca agggcctaac agcatctatg gtcaacaatt atgtgaaaaa
     9121 tggttacctg acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat
     9181 tgctatcaca accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac
     9241 tctacaaact caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca
     9301 aggaattgac ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca
     9361 tcaaactcta gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt
     9421 aaactcagca aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg
     9481 ggtgcagtca tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac
     9541 ggatttttat cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta
     9601 tcatccacca tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc
     9661 attgaccatt ctatgattta cgttgccatt gccggctcat acacgcccgt tgtcttgacc
     9721 ttgatgaata actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt
     9781 ggtattctct ataaaatctt tgctaaaaag gtcaatgaga aatttagcct tgctctttat
     9841 ctgattatgg gctggttggt tctggctatc attcctgcca ttatcagtca aacgacaccc
     9901 gttttctgga gtctcatggt aactggcgga ctctgttata cagttggagc tggattttat
     9961 gccaagaaaa aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgca
    10021 cttcaataca tcgctattgt ttattacatg taaaaaagtt gagaaattca atctcaactt
    10081 ttttctttac acatattgat aaagtactgg tgcaagcgca catcatcagt caattctgga
    10141 tgaaaagaac ttaccaacat atttttttct tgggctgcaa caatttgatt gttcactgtt
    10201 gctagaattt ctacaccctc accaacacta ctgataatcg gaccacggat aaaggtcatt
    10261 ggaatcttgc caactccctt acattctgct tccgtgtaga aacttcctaa ttggcgccca
    10321 taagcattac gctcgaccac catatccata gttcctagat gactctcttt ctgagaagtg
    10381 atttccttag ccagcaaaat taagcccgca caggtcccaa acactggtaa gccagataga
    10441 atggcttctc ggatgggaag tagcatgttc tggtcacgta agagcttgcc catggttgta
    10501 gactcaccac caggcaaaat caaacccgac aagtcactct gatcttgctg aaaatcatct
    10561 agatttctga gttctacact ctcgacacct aattgatcta gcacttttgc atgttctgca
    10621 aaggcccctt gcaaggccaa tattccgatt ttcatctatt ttcctcgttc agccatgaga
    10681 atttggattt cattttcatt aataccaacc atggcttctc ctaaatcttc agagatttga
    10741 gctaggattt gaggattaca gaagttagtc acagccttaa caatggcact cgctcgttta
    10801 acaggatctc ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc ccctaattgc
    10861 atcattaacg cagcatctgc tggcgttgca acacctccag cagcgaaatt tacaactggc
    10921 aattttccat gttcatgaac atattggacc aattctacag ggacttgcaa atccttggca
    10981 gcaacataaa gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg attcatcata
    11041 cgcatatgac gaacagcttg gacgatatcc cctgtccctg gttctccttt ggtacgaatc
    11101 atggaagcac cttcagcgat acgacgcaag gcttcaccca aatccttagc accacagaca
    11161 aaaggaactt ggaattcttt cttgtccaca tggaaacggt catcagctgg agatagaact
    11221 tcactctcgt cgatataatc aatttcaata gcctctaaaa tctgagcttc aacaaaatgc
    11281 ccgattctga ccttagccat tactggaata ctaaccgctt cttggatttc cttaatcatc
    11341 tttgggtcgc tcatgcggga aactcctcca gctgcacgaa tatcagccgg aattcgttcc
    11401 aaggccatca cagctgccgc accagcagct tctgcgatac gagcctgttc aggattctgc
    11461 acatccataa taacaccacc cttgagcatc tgtgccaagt ttttatttag ttcataacga
    11521 ttttcagtca tttgttttat cctcatcatt tgtattggta gctacctttt cattttaagt
    11581 cagaacagaa tgaagcacaa gataaaaaaa ctataattgt atggggacag attggctaaa
    11641 aactatctga ccttaactta aggccagata gctcattcat ttttatcttt cagccgtaag
    11701 ggcagccatt gtgatgtagt tgtatggttt attgaagtgt ggcaagaaga agaggtctgt
    11761 caatgccaat ttatcaattg tcacatgctc ttggatagca agtgagaaca tgtggattcc
    11821 catgctaatt gcaatatcat gtgaaaccat ttgggcacca agaatttcac ggctatcttt
    11881 gtcaaagaca atcttaattg ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg
    11941 aagatcgtta aagcctgttt cagttgcgtt gtaaccagca gctttcgctt tttcaagagt
    12001 caaaccagtt gaaaccatgt gaagaccgta gattgagata ccatttgaac cttgaacacc
    12061 gattccttcc aattcatgtc cacaagcgtt gtaggcacca acgataccag tgcgaacagc
    12121 atttgaagca agagcgatat agcttgtatc tttacgagca ttgtcataaa cagtcgcaca
    12181 gtcaccaaca gcgtaaacgc ctgggataga tgtttcttgt ttcttgtcta caaggaaggc
    12241 accgttgcgg aagagttcga tcttaccatc tgcaagggct gtgtttggac ggaaaccaac
    12301 tgcaaggata accatatcca cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt
    12361 accgtcacct tcgattgctt taacagtttg acctagagcc aagcggatgt tgtgatcttc
    12421 caagttcttc gccatcattt gtgtgaagtc tttgtcatag taaccgttca agacagtatc
    12481 aacgatatca acaaggacaa cttcttttcc aagacgttca aaggcttcag caagttcaac
    12541 accgatgtaa ccaccaccaa caacggcgat acggtcgagg tgttggctct tgtcagaaag
    12601 tttattgata acttcttcag cattttggta caatttcacg aattgtacgt tttcaagagt
    12661 tgctttaaat tcgcggtttc ctttaacaat ttcaacacct tcgattggtg gcaagattgg
    12721 tgtagaacct gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt ttccttcaac
    12781 ttccgctgta actactttgt tatcatagtc gattgaaaga acaggtgagt tcatgtaaac
    12841 tttagcacct ttagcttcca atttttcttt atcagaatag aacaagcctt cagcaccgtc
    12901 aatttgttca ccaatccaaa gagccattcc acatcctagg aaagagatgt tagagttttg
    12961 gtcaaataca acaatttcgt tctcatttcc aaaattatcc aacatggtat tgatacatgc
    13021 tgtaccagcg tggttagcac cgactacaac gattttactc ataaaaattt cctgccttca
    13081 ttctaaattt accttactag tataccattt accgttagcg tttacaaggg gggtactatt
    13141 atttcagcat tttttatcaa aaataattcc ccatttatcc tgttttcaag cctcatgaat
    13201 tttatgagca tatttcttga ctttttccaa attttgtttg tgaaaattct aactttatgt
    13261 tattataata acatgaaaat gaaatgtaag ggcttcaatt tagatgatta tttgatatta
    13321 caacttattt tctataagag aaaggagttg gtagcttgac tcttcgttca cattctgaac
    13381 ggctaatggg gactactatc actatttcat tagtggatga gcaagccgat atctttctcc
    13441 aaaaatcctt tgacttgctc aaagaacttg aataccgctt caacgccaat agtcaagaat
    13501 ctgagttgat ggaaatcaat tatcaagcag gaatagcccc cgtcacggtt catccagacc
    13561 tgtttgagct gatttcactt gggttagagc atagcctagc gctctctagc cacctcaata
    13621 tcagcattgg tcccttaatt caaacctggc gtatcggttt ttcagatgcc aaggtcgccc
    13681 agcctcaaga aattgaatcg gtgctgcctc taatcaatcc tcatggtatc gagttagatt
    13741 cttctacttc tactgtgttt ttaaaacaga aaggaatgaa gatcgatcta ggttgtttag
    13801 ccaaaggata cagtgcggat aaggttgccc aatttcttag gaaagagggg gtgacttctg
    13861 ccttgatcaa tctgggaggg aatatcctga ccattggaaa aaatcaggca agaggggata
    13921 acccatggca aatcgggatt caagacccag ccaatcctag gggaaatcat ttaatgacca
    13981 tccctgttgt caataaatct gtcgtgactt caggcattta tgaacgtcac ctgaccgtcg
    14041 atggacaaga ttaccatcac atttttgaca gtcaaacagg atatcctgtt gaaacggaac
    14101 tagcgagtct aacaatcatc tctgataaat cagtcgatgg cgaaatctgg acgactcgac
    14161 tctttggaga aagacctgct tctatcctct ggcaagtcga aagtttggag ggcatcgaag
    14221 tcatcctcat cgataaagaa ggccacctaa gctgttcttc aggaattcct actctataga
    14281 gacaactgtt tcaatggaat tttaaaattg tattatgtaa aaagagaaag gaaatctcat
    14341 gttaaaactt attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtcaattgct
    14401 tcaatacatg caaaaacact ttgctgacaa agctgaaatt gagcttgttg aaatcaaggc
    14461 cattcctgtc ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc
    14521 tgctaaaatc gaagagacag atggcgttat tatcggtact cctgagtatg atcactctat
    14581 tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa
    14641 caaaccaatc atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt
    14701 gcaacttcgt caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt
    14761 cttgctctca cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt
    14821 tatcaagaaa ttggatgcca tctttgatga cttccgtatc tttgtaaaaa tcacagaaaa
    14881 attacgtaat gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt
    14941 gtaagatagg agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca
    15001 atcatataac cgtaagctct tggaatttat ccgtcgctac ttcaaactca aatttgaatt
    15061 agaagttctt gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag
    15121 tttccaatta cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac
    15181 tcctgagcat aaccacacta tctcagtttc actcaaatct gtacttgaat ggctttcata
    15241 cgaagttcat ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca
    15301 aggaacatca cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtgttaatgc
    15361 ctacacgctt ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa
    15421 cggaaatatc accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt
    15481 taaatacgta ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt
    15541 ggattgtgga aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg
    15601 ggtagaaaaa gttgcagcaa tcactggtgc tgtttctggt gatacctatg tcaaattgga
    15661 ccacggtatc ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt
    15721 gacatacgct gacgacaaca accaattcct ctactacaac aacgctcacc aagatccaga
    15781 cactatgttc gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg
    15841 ttctcttcca ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg
    15901 aaaccaagaa tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca
    15961 caactaccaa gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt
    16021 taacttccaa ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg
    16081 tagcggacca tttgctcctg ctgccggagg tcatggaaac gccgatgcta cttctggcgc
    16141 ttctgattca ggggatgctg gaggccacgg tggtgacgca gatgctacag ccggtgcaag
    16201 ttactaatga tagtttttag gaaccattat ggttcctttt ttagcgaaaa aagactagca
    16261 aacacaaact gctagtctct ttgttatcgt ttcatctatc tttgttaaaa gaaaaataca
    16321 tattatgaat ttgggtcatc aagactacgg ccatgtaatc ctttttcacg ttgtacttgg
    16381 cgtagttttt ctggtgtaac atcgtttcct ttttcgtcca caattttgat tccttcaatg
    16441 tgatgacgaa cagcgcgacg ataaccttcg atgtactcct cacgtagttt ggcttgttcc
    16501 actttttctt ctggtgttaa gccttctgtt tttttctttt tagcaagctc attgatacga
    16561 gcaatttttt tcggatccat ttcttctcct ctgtaataag ataaagtccg tttatcaaat
    16621 tttatttagt gttaatttgg ttaaaacaag caaacttagc tgctttcttg gttagttgtg
    16681 acaagattgc caaacgattt tgacggacag cctgatcttc agccattacc atagtatttt
    16741 caaagaaagc atcaatgact gggctaagcg caaaaagttg tttcaattgc tgacttgcag
    16801 gtcctgataa aatgagtgtt tctactgctt ctgccaaagc tttttcttgg tcattctcaa
    16861 atagtgctga atcaaccgta gcaacccctt ctgccttctc agccaggtta aaggcacgag
    16921 aaagtgattc aacagatggt ttaaaatctt cttccttgct tacttctacg agagcacttg
    16981 ctgcttccaa catatctgcc acaacaaagt ttgaacctgc aagaactgct tccttgatat
    17041 cttttggagt agagcccatc atcttatcaa cacgagcctt gataaagtcc ataacctctg
    17101 ctttattttc ataagtcaaa ctgtcaaatt tcaatgcata aaggctatca atcagctcat
    17161 ccatagcaat gtgccaacca aaggcatcca agatacgaac cacaccttga gttgcacgac
    17221 gaagggcata agggtcatta gaacctgatg gaatcaatcc tactgagaag aaactcaaaa
    17281 tcgtatccaa tttgtctgca atggctagaa ctgcaccgac cttgctctct ggaagttctc
    17341 cttcagctga tgtaggcatg tagtgttcac gaatagcagc tgccaccgct ggagtttcac
    17401 cagcaagaag ggtgtatttt tcacccataa ttccttggag ttcgtcaaat tcaccaacca
    17461 tacctgtcaa caagtcaaac ttgtaaatgg ctgctgcacg agcaaggtca actgtttcat
    17521 ccactgacaa accagctttt tctgccaaaa gtacagtgat ttgacccgta cgaatcatgt
    17581 gttcacgaag ggaaccaatc ttctcatgga aggtaacatt gtttaatttt tcaacaagat
    17641 ctgaaatcac caatttttgg tcttcacgcc agaagaattc tccgtcttcc aagcgggcta
    17701 ccaagacttt ttcatttcct ttaatgacat ttttcaaacg ctctgcgttt ccgttacgaa
    17761 cagaaatgaa gtttggcaag agttttccat cttgatcacg aacaacaaag taacgctggt
    17821 gttccttcat agaagtcacc aagacttctt ctggaacttc aaggtattta gcatcaaaac
    17881 ttcccatgaa ggcagttggg tattcaacca aattcaagac ttcattcagc aaatccgcat
    17941 caatttcgat acgtacacca tgttttgcct caatttcctt gatttggtca acaatcattt
    18001 gctcacgttc acatggatct gcgattacaa actgcttacg aaggtcttct tcatagctca
    18061 atgctgactg aattttggtt tctttcccca agaaacgatg gccacggctc acacgacttc
    18121 ccttgatatc aaggaaatcc aagtcaaact cttgctcatc caagagaaca gttaaagtgt
    18181 gaacagggcg gatgtattca aagctatttc cagcccagtg catgctgaca gggaaagtca
    18241 atgacttcaa gacatctaca acaccaggaa caatggcttc aactgattgg ccaacttctt
    18301 ccttagtgac atagacatat tcttcaccct taatttcacg gaattcgata tcttcaacag
    18361 tcaaaccttt cccacggaca aatccttgag ctgctttggt gaagtttcca tcactatcca
    18421 aggcaatttt ctttgctggt cccttgaaat cttctgtcaa atcagactgt ttgtctgata
    18481 aaccagtcac acgaacagcc aaacgacgtg gtgttgagaa ggtttgaatg gcttcaaaag
    18541 acaggcggtt ttccttgagg aaggctgcca ttttttcgcc tagttgtttt tcacttggtg
    18601 tgacaacata ggctggtaat tcttcaagac cgagttctac taataagttt tttgtcatgt
    18661 tttttgtcat gttttttctc cgaaaagtat ctttttattt tccagtttgc cttatgtgat
    18721 tggcacgcaa gcaaccaact tcgttgtttc attgctaaaa ttggagccaa aagtctccaa
    18781 ttttgcctag tatccttagt ttcactaagg ataccttcat cactgcggca actggctcag
    18841 ggctcactct gttcgcccat tttcgtaatt tgtcactctc tttattctgc gtcttctgct
    18901 aggagtttag ctcttgtttc ttcatccaaa agtgggtagc ctaggcgttt gcgttctgcg
    18961 acaaaggttt tggctacgac acgggccaag ttacggatac gagcgatata gcctgcacgc
    19021 tctgttacag atacggcacc acgcgcgtca agcagattaa aggtatgtga acatttgaga
    19081 acatagtcat aggcagggtg taccaagcct tcttctaatg cacgaccagc ttctttttca
    19141 aacttatcaa aattttcaag caacatttct tggtccgaaa tttcaaatga atattttgag
    19201 tgctcatact caggctggat aaagatttct ccgtatttta caccatcagc ccactcgata
    19261 tcatagacag agtctacttc ttgaatgtaa gaagccaagc gctccaaacc ataggtaact
    19321 tccgcagtca cagggctagt tgccaatcca ccgacttgtt ggaaataagt gaactgagtg
    19381 atttccattc cgtcaagcca aacttcccaa ccaagaccag ctgaaccagt tgatgggttt
    19441 tcccagttgt cctcaacaaa acgaatatcg tgctccaaag gattgattcc caatttttcc
    19501 aaagactcaa ggtaaagttc ttggatattt gatggagaag gcttcatgac cacctggaat
    19561 tggtggtgtt ggtagagacg gttagggttt tccccataac gaccgtcagc aggacgacgt
    19621 gatggctcta cataagctgc attccatggc tcaggtccga tagcacgaag gaaagtgtaa
    19681 ggactcattg ttcccgcacc tttttcattg tcataagcct gcataagcat acaaccttgg
    19741 tcattccaaa atcgttgcaa agtcaaaata atttcttgaa atgttaattt cttagacatt
    19801 gaatactcct taataaaaaa taatttcttg cgacacgagt ctacctcgtc gattatacga
    19861 ggcaagacga gttagtactg cgtctagctc agcaccctag tgctgagcgt ggggcagtcc
    19921 gactgtaagg aggtctctgc cactgatgca gtgaaatgtc aatttcttag acattgttta
    19981 ctcctttagt ttttatattt ctttttcaat taatttctgc tgaacaaacc aatctaacaa
    20041 gggaaaagtt ttttctgtat ttgcccataa atgataagaa tctaagaccg cttgtacact
    20101 acctaaattt tctaccaact tatctactgt caaaaagctg cgccagtatt cataaaaaat
    20161 acttgcatag ttcttatcat aagtcgaaga accaaaatca ttcaatgaat gccaaccata
    20221 cttcttctga aaaagttcta cgagagattg attacaaatt ttttccgctt gaaattcctc
    20281 ttctgtcaag aaatacttgc gactaatata ttcaaccatc ccctcttcga accagatata
    20341 agagtcataa ccatcaaaat catctaaaaa atgctccgac caatgagcta actcatgtcc
    20401 tacaatctgt aagaggaaat tttcagataa agaatgatag tgactttcta ttgcttgagt
    20461 ttgctgagaa cactcgtagt ccatcaattg atacaaatac aactctttcc aaacagctaa
    20521 atcaggcgcc ataaccattc gtctattatt tgtataggct ggaacagcgc tttccctaat
    20581 aatctgtgta gcagcattaa aatctgacca aataacggct tgcggtaaat cacagactgc
    20641 aagttcattc tttaaaaaag attgataatc tttcaacttt tcagtatttc ttactacaaa
    20701 atcacgaaat gtagctagtt gactatcgtc ttttacaaga taaaggttgt ccacggctct
    20761 cctttctttc acacaataga ctgaataagc aaaagaacca catctgacaa tcctaggttg
    20821 atttaaccta ggggcgtcag aaacgcggtt ccaccctaat ttattacttc attgtttttg
    20881 aaaatactac agaaagcgcc agccctttat tttgccctac ttggctccca ctatcccaag
    20941 ctcgcttgaa gaacgccata agactttctt tcctgctgac tattatatca aaaattagaa
    21001 taatgtacaa ttctttttat gattttctag aaatccacat catcaactcg tggagcaccc
    21061 gattgaacag caatcgtttt taaggtttct ctctcctcat gactcagttc aattccaaag
    21121 caatcaagat tagcttgaat acgagaggtt gtgatagatt ttggaagtgg taaaaatcct
    21181 tctgccaagc tccaggccaa ggctatctga gcaatcgatt ttccgtgatt tgctgcgatt
    21241 tcttgcactt gcttgctatc aaatagttct ccctgaccaa aaggccccca agcttccaat
    21301 aaaattccct tttcacgaca gtaagctacg acttgatctt gatacacacc tggcgccaag
    21361 cgaacttgat tgaccgcagg aacgatagtt gcagtttcaa gcaaggcatc caaatgatgg
    21421 ggaagaaaat tgctaacgcc gatagcacgg attttccctt cttgatagag gtcttccatc
    21481 gctctccaaa cttccgcatt gcgagttttc catgcgtcat tttctctgag cggttttggg
    21541 ttcggccaat gaatcaaata caaatccaaa taatccaagc ccagtttttc tatagatttt
    21601 tccaaagctt gacgagtttg ctcataggtt tgctgactat tccaaagctt ggtagttacg
    21661 aacatttctt cacgtggaac tccgctatct ttgattgctt gaccaacact ttcttcattc
    21721 tgataaatcg ccgccgtatc aatatgacga taaccagcct tcaaggcttc taacactgca
    21781 cgataggctt cttctccatc cttagcctta aaagttccaa atcccaatac tggaatttct
    21841 actccattat ttaattgata ggtattcata tgcttctcct tctattttat tcatcaatta
    21901 tagcataaaa cattttttaa gtagtactat cttctgtaaa aaataagtct gtcaaatatc
    21961 taacagacct attttttctt gcttattcat cacgactata gtacagctca tgagctttta
    22021 ggcgagtatt gagcatctct ggtatggtca caaaggtata accttgattt ttcaaatact
    22081 caatgaccct tggcaaggca ttgactgtcg gactgtgaat atcatgcatc aaaacgatag
    22141 agccgttagc tacttgatgc tgaatttctg tcaaaataga tgcttcattt ttactcttcc
    22201 agtccagact atccacatcc cacatgataa agctcaaatc caagctattg cgaatatcat
    22261 ctgtaatagc accataaggt ggacgcatga gtttagaact agaacccagc actttagtta
    22321 gcacatcctc agtatcagta atctgctttt tagcttcatc aagagagagt tgcgagagaa
    22381 tcggatggct ccaactatgg tttccaacaa catgaccttc agattttatc ctcttcacca
    22441 agtcctcatt cccagaaaca tttttcccaa gcacaaagaa agtcgcttta atatcatatt
    22501 tagctagggt ctctaatacc tgcggggtcg ttgctggatt tggaccatca tcaaaggtta
    22561 gagcgacaac tttttgatat ttcttatcaa agtaagattg gtacaaggcc gcatcttttt
    22621 cgagtaagta cgaagattgg ataacatcaa agaaagcaga tactggcaag gctatctctt
    22681 ctaaattttc aaccacagga cttggataaa ggataatctg actatccttg taatcaaaat
    22741 tccatgcaga caagtcttgg tcagagaagt tttttacaat ctgctcactc tggtcttgct
    22801 ctattttttt atcctctatg aaggaggtca actcttttat cagctgttcc ttagccttac
    22861 tagcatctga aaacagttgg tcaagtgtaa aaggttgccc atcttctgtt aagtgaacct
    22921 ttcctagact ggtcttttca gtctcttcaa tcttaaaagc aactaaatca tagatttgtt
    22981 tggtcacatt acgattaacg actcccttta aacctgactc ttcttgctct gtatagtaga
    23041 aaacaagatt gtccttactt tctagcttgt ccttaacatc ttgatttatc agctccctaa
    23101 cagaggaaat cactttctcc ccttggagag gataataggc aatcacttcg gcttgcccct
    23161 gacgaaaatg ctccttctga tttccctcac tcaattgatc atctttctct tttttgagcg
    23221 attcaatctt ttgctcaaaa cttttttgct ggtagatctt aaaagcaata aatccgccta
    23281 ataggcaaat agaaattgct aagataccaa tcaaagccaa taatacatgt ctcgttttcc
    23341 cgtgtctgcc acgtcctagt ctacttttat tcatatttat atcataacaa atttgagcaa
    23401 taatttcaat gatacaattt ggcaatccac aattcctcta gcaagccaaa gcatcttaca
    23461 tcaactccaa catccgtatc tgtagagatt atgtagtttt ctatctatgg aatttctagt
    23521 agattgaagc tagaatagta gacct
//
LOCUS       DAVLTM010000019        22686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLTM010000019 DAVLTM010000000
VERSION     DAVLTM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.019"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            86..274
                     /locus_tag="VYN16_000664"
     CDS             86..274
                     /locus_tag="VYN16_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1403235.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            336..1094
                     /locus_tag="VYN16_000665"
     CDS             336..1094
                     /locus_tag="VYN16_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1403236.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            1152..1613
                     /locus_tag="VYN16_000666"
     CDS             1152..1613
                     /locus_tag="VYN16_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1403237.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRF"
     gene            1647..2006
                     /locus_tag="VYN16_000667"
     CDS             1647..2006
                     /locus_tag="VYN16_000667"
                     /inference="COORDINATES: protein motif:HMM:NF015692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1403238.1"
                     /translation="MNDNPIECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQET
                     VPQAIIAFLESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLD
                     EPLKDVVRRWENRIKVY"
     gene            2118..3002
                     /locus_tag="VYN16_000668"
     CDS             2118..3002
                     /locus_tag="VYN16_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1403239.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKQGILA
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3165..4904
                     /locus_tag="VYN16_000669"
     CDS             3165..4904
                     /locus_tag="VYN16_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1403240.1"
                     /translation="MELSAIYHRTESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            5179..5481
                     /locus_tag="VYN16_000670"
     CDS             5179..5481
                     /locus_tag="VYN16_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403241.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            5537..5965
                     /locus_tag="VYN16_000671"
     CDS             5537..5965
                     /locus_tag="VYN16_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403242.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
     gene            6023..6632
                     /locus_tag="VYN16_000672"
                     /pseudo
     CDS             6023..6632
                     /locus_tag="VYN16_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            6914..7161
                     /locus_tag="VYN16_000673"
                     /pseudo
     CDS             6914..7161
                     /locus_tag="VYN16_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            7266..7568
                     /locus_tag="VYN16_000674"
                     /pseudo
     CDS             7266..7568
                     /locus_tag="VYN16_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            7724..8953
                     /locus_tag="VYN16_000675"
     CDS             7724..8953
                     /locus_tag="VYN16_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1403243.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            8973..11669
                     /gene="ppc"
                     /locus_tag="VYN16_000676"
     CDS             8973..11669
                     /gene="ppc"
                     /locus_tag="VYN16_000676"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1403244.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    11752..11969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12082..13086
                     /locus_tag="VYN16_000677"
     CDS             12082..13086
                     /locus_tag="VYN16_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1403245.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            13155..14021
                     /locus_tag="VYN16_000678"
     CDS             13155..14021
                     /locus_tag="VYN16_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403246.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            14018..14776
                     /locus_tag="VYN16_000679"
     CDS             14018..14776
                     /locus_tag="VYN16_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403247.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            14866..16626
                     /gene="dnaG"
                     /locus_tag="VYN16_000680"
     CDS             14866..16626
                     /gene="dnaG"
                     /locus_tag="VYN16_000680"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1403248.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYQAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEEFERLISQKRRME"
     gene            16629..17738
                     /gene="rpoD"
                     /locus_tag="VYN16_000681"
     CDS             16629..17738
                     /gene="rpoD"
                     /locus_tag="VYN16_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1403249.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            17752..18081
                     /locus_tag="VYN16_000682"
     CDS             17752..18081
                     /locus_tag="VYN16_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1403250.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            18196..19239
                     /gene="cpoA"
                     /locus_tag="VYN16_000683"
     CDS             18196..19239
                     /gene="cpoA"
                     /locus_tag="VYN16_000683"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1403251.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            19255..20580
                     /locus_tag="VYN16_000684"
     CDS             19255..20580
                     /locus_tag="VYN16_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1403252.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRIIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            20611..20787
                     /locus_tag="VYN16_000685"
     CDS             20611..20787
                     /locus_tag="VYN16_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1403253.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            20846..20980
                     /locus_tag="VYN16_000686"
     CDS             20846..20980
                     /locus_tag="VYN16_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1403254.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            21044..22354
                     /gene="obgE"
                     /locus_tag="VYN16_000687"
     CDS             21044..22354
                     /gene="obgE"
                     /locus_tag="VYN16_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1403255.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYSRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            22364..22522
                     /locus_tag="VYN16_000688"
     CDS             22364..22522
                     /locus_tag="VYN16_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403256.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         7206 a         3748 c         4982 g         6750 t
ORIGIN      
        1 cctctccact tttaaactaa tgtattataa aagccagttg acacaagtgt tacacctgtg
       61 atagaattaa cttgaggtga tgaaaatggc aaccgataaa aatagaataa tgattagctt
      121 ggatgataaa aatctagaga aacttgaaaa tttagtagaa gatgctagag atagaagggg
      181 aatgcgacta acaaagtccc aggttattga attactccta aataccgttg attatttcga
      241 tgatattatg ggggctattt actcaaaaaa ataagagcaa aattgctatt attttttaca
      301 aattggtgta acactagtga tataggagtg atgaaatggt tgtttatatt agacaaagta
      361 aattaccaag tgaggtttca attaataaat acaatgctca agtaggtgcc tacttacaag
      421 gagaagaagc tgttttatat caatcttttt ctgaaataaa agagttaaca agtgaagata
      481 tagttgtaga ttatataatg gagactagag cattactaaa aatgatgggc ttaaacgttc
      541 cggttcatga ctatcctatt gagcttaaag agttttatgg tcgaaagatt tatgctggta
      601 ttttaggaga gattgtgaat atacctgata attggggaaa atttattaag cctaaagctg
      661 gttcaaaagt ctttactgga agagttgtta atggaaccca tgatttaata ggtattggtc
      721 tacctttcga ctatcctata tggattagtg aggttgtaga attcatagct gaatggcgtt
      781 gttttgtgtt agatggtcgc gtattagatg ttcgacccta tacaggtgat tatcatgcac
      841 aatttgatgc aagtgtaatt gatgaagcga tatcatgttg gaaagatgcg ccaatagctt
      901 atggactaga tatcggtgtt actcgcgatg gcagaacact tgttgttgaa gtaaatgatg
      961 gttatgcatt gggaaattat ggcctatctc ctttaaaatc aatcaatttt catagagcta
     1021 ggtggaaaga aatggtaaaa ccctattttg aaaaaaatga aatttttaaa atccaacagg
     1081 atgttgtttt ctaaaataaa tatggtatat aaccaatata aaaagcatca aaatgaacaa
     1141 ggaggaatct gatgcttgga gcaattattg gagatattgt cggttctgtt tacgaatgga
     1201 acaatatcaa aacaaaggac tttcctttat ttcggaagga ctgctttttt acagatgaca
     1261 cggttatgac ctgtgctgtt gcagaagcaa ttatgaatgg tggacaaaaa gacgacttca
     1321 ttgacgcgat gaagaaatat ggcagaatgt atccgaatgc tgattacggt gctcggttta
     1381 atgcatggct aaacagcgat aaccgtgagc cttataatag ctttgggaat ggatcagcta
     1441 tgcgtatttc tccatgtgct tggatcatgg actgtggttt ttatgcgaaa actggtatat
     1501 ggccatcatc tagaggactt acgagtcttt ctgcagaggt aactcataat catccagaag
     1561 gtgtcaaagg cgctatggct acagctgatg ctatctttct atgtcgtttt taatttggtg
     1621 gttattgtag agagtacgag taatcaatta acgacaatcc tatagagtgt aaaagacgaa
     1681 ttaaggatta tatagaaaaa gaatacgatt acaatctatc tcaaacttta gatgaaatcc
     1741 gtcctaacta tcgttttaac gaaacatgtc aggaaactgt ccctcaagcc attatcgcct
     1801 ttcttgaaag tagagacttc gaagatgcga taagaaatgc catctcactt ggtggcgaca
     1861 gtgatactct cgcagcaatc acttgtagca tagcagaggc agcttacggt attcctgatt
     1921 ggatcaagga taaggcctat tcttacttgg atgaaccctt aaaggatgta gttaggcgat
     1981 gggaaaatag aataaaagtg tattaatctc cagtacgtca gatttctctt ttctgtattt
     2041 tcattgtata ataggattac atttacttgc atacgacgaa atgtaaacat ttctatttaa
     2101 aattaaggat aaaatcaatg aaaaaagcaa tggtaattat caaccctact tctggtggcg
     2161 agaaggcttt ggattacaaa gaaaagctgg agaataaagc aaaagaatac tttgaatatg
     2221 ttgaaaccaa aattaccgaa aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc
     2281 gtgagcagta tgatgcagtg gttgtgtttg gtggagatgg gactgtcaat gaagtcattt
     2341 caggtattga tgagagagac tacattccta agttagggat tatcccaggc ggtacgggta
     2401 acctcattac aaaacttttg gaaatcaatc aagacatcga tggcgcaatt gacgaactgg
     2461 attttgattt aaccaataag attgatatcg gtaaagcaaa tgacaactat tttggttata
     2521 tctttagtat cggttctctg cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa
     2581 aacaaggtat tttagcctat gctgtaaata ctatgaagtc tgtcatgaca gatcaggtct
     2641 ttaacattaa ggttgagaca gaaaatggaa attatgttgg tgaagctagc catgttttgg
     2701 tccttttgac aaattacttc gctgataaga aaatctttga agaaaacaag gacggctatg
     2761 ccaacatttt gattctgaaa gatgcctcta tattctccaa attatccgtc attcctgatt
     2821 tattaaaagg ggatgttatc gcaaatgata atatcgagta tatcaaagcg cgtaatatta
     2881 aaatctcttc agatagtgaa ttggagtcag atgttgacgg agataaatca gataacctac
     2941 ctgtagaaat caaagtccta gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt
     3001 agtatataga gaaagccttt tttaaggctt tttgtatact ttaaaagata gttcctttaa
     3061 caacggacat tccttgcaaa tagttcacaa aaatagtata ctggattcat tgagtttgaa
     3121 aacgtttgcg taaaatttga atgaatactt taggagataa attgatggaa ttgagtgcta
     3181 tttaccatag gactgagtcg gagtatgcct atctttataa ggataagaaa ctccatattc
     3241 gaattcgaac taagaaaggg gacattgaaa gcatcaactt gcactatggg gaccctttta
     3301 tctttatgga ggagttttat caggatacaa aagaaatggt caagataact tctggtacct
     3361 tatttgacca ttggcaggtt gaagtgtcag ttgactttgc acgtatccag tatctttttg
     3421 agctcagaga tacagaaggt caaaatattt tgtatggcga taaagggtgt gtggaaaatt
     3481 ctctagaaaa tcttcatgca attgggaatg gatttaagtt gccttatctt catgagattg
     3541 atgcctgcaa ggttcctgac tgggtttcaa atacggtatg gtatcagata tttcctgaaa
     3601 gatttgccaa tggcaatgct ctattaaacc cagaagggac tttagactgg gattcatctg
     3661 tcacacctaa gagcgatgat ttctttggtg gtgatttaca ggggattatt gatcatatgg
     3721 attacttgca agacttgggt attactggac tatatctttg tcccatcttt gaatctacaa
     3781 gcaatcacaa gtacaatacg acagattact ttgaaattga ccgtcatttt ggagacaagg
     3841 agacctttcg ggaactggtg gatcaagcgc atcatcgtgg catgaaagtc atgctggatg
     3901 cggtatttaa tcatattggt tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggtg
     3961 aacagtctgc ttataaggat tggttccata ttcaacaatt cccagtgaca actgaaaagc
     4021 tagttaataa gagagactta ccctatcatg tttttggttt cgaggactat atgcctaagc
     4081 taaatacagc caatccagag gtcaagaatt atcttttaaa ggttgcgact tattggattg
     4141 aagagtttaa tatcgatgct tggcgtttgg atgtggctaa tgagattgac catcagttct
     4201 ggaaggattt tcgtaaggca gttttagcta aaaatcctga tctttatatc ctaggagaag
     4261 tctggcatac atctcagcct tggctaaatg gagatgagtt ccatgccgtc atgaattatc
     4321 ctttatctga tagtatcaag gactatttct tacgaggaat taagaagaca gaccagttca
     4381 tcgatgaaat caatggagag tctatgtatt acaagcagca gatttcagag gtcatgttta
     4441 atctcttgga ttcacatgat acagagcgaa tcctgtggac ggccaatgaa gatgttcaac
     4501 tggttaaatc agccttagcc tttctctttt tacaaaaagg aacaccgtgc atttattacg
     4561 gaaccgagct agccttgact ggaggaccag atccagattg tcgtcgttgt atgccttggg
     4621 aacgtgtatc aagtgacaat gatatgctga actttatgaa gaggctgatt aagattcgga
     4681 aatacgcgtc agtaatcatt tcgcatggca agtatagcct tcaagaaatc aaatctgatc
     4741 tagtagctct ggaatggaaa tacgaaggac ggatcctcaa agcaatattc aaccaatcaa
     4801 cagaagatta tcttttagag aaagaagcag tagcactagc aagcaattgc caagaattgg
     4861 agaatcagct tgtcatctct ccagatggat ttgtgatttt ctaaaaacta gttgatgaag
     4921 attatggtac atttcatatc ttatatagta taataaggct agttactaaa cttgtaaagg
     4981 agaacttaaa tgaattgtag aggacacgaa acaagacaaa gaattgttag agattttgaa
     5041 gtttagccta aagcacatat taagctgtta gcaaatcaac aaaaacatag tgatgcagga
     5101 gcaactattg aagatgaata ttatgtattt atcgctgaga gtaaaattga tggcaagaag
     5161 gaagttattc agtgttgcat gggtgcggca agggattttt tagaactaat taatcacaaa
     5221 gggctacctc tttttaatcc gcttgtaggt gattctcatg taaataatag acaagaatat
     5281 gacaatacag ggagtggaaa tttacaacct gaaaagtgga atgaaactgc aaagcagctt
     5341 tataatgtta taatgtggtt gattatttta tggaatgcta agccggatac acctttattt
     5401 aattttaaag acgaagtaat taagtataaa acatatgagc cttttgaaag cagtataaaa
     5461 ttggtatcaa tcaatgagta acaaagtatc agcatgattc gaaacattta cttgtaattt
     5521 gcggaggaac atcaatatgg atggaataac aaaagatttt ataaaaacgg ctaaactaat
     5581 gaaacaatta tggccccaat tgaccgataa agaagctatt gatgaagtaa aaaaatatac
     5641 gaatggcaaa aatactgcaa tctttactga agttgaaggt gacacaattg taggtctagc
     5701 actatgttca ctcagatttg attatgttga aggttgtaaa tatagtcctg ttggattctt
     5761 agaagggatt attgttgacg aggaatatcg tttaaaggat attgctaaaa atctctgtac
     5821 aaaatgtgag gaatgggcga aaaataaagg atgtaaggaa tttgcaagtg actgtacttt
     5881 aacgaatacg gattctataa gatttcatct caatattgga tttcaggagg caaatagaat
     5941 tattcatttt aagaaaaaat tataatttag aaacgtgaat aaacttgttt aaactttatg
     6001 tatctttaga aagtgtgtta acttgaaaga acaaattttt ttgatgggtg ggaatccccc
     6061 aatgaagaaa tatagtattg ttgataaaat tgtattatca accaagatta aaagaattat
     6121 catttttaca gtttttcgag aaaattggga accgtacatg aaaaagtaca cggaagtttt
     6181 tcaaagtcaa tttcctaatc taaacattga ttacttgctc ctggacactg agcagattga
     6241 tcttgatagc tatctagatg ctgacataat tatcatcggt ggtggaaata cggaaaaata
     6301 tattgctaac ttatgttaat caggagttca aaagttatat cgatcatatg cttaataaag
     6361 aggcaaaaat tataggattt tctgcaggag ccctactatt aggagaaaaa gtctatgtct
     6421 cacctaatga taattcagat catcagataa agataaaaaa tggattagga ctctttagtc
     6481 agtttttaat tagtgtccat tatgattcct ggaatgataa agcaaataag gatagagctg
     6541 aagaactcgt taatgttccc ataattccac taaatgatca ttcttgtctt gtattggata
     6601 aacttggaaa catcatagag aaaattgact aactttcata atgtaaaaat ggatctagga
     6661 aataaaagtg tagacgaact ggataggtta ctagtgaaaa ctgcaaaaag tgttgtaaag
     6721 tgaatcggag tgtgcttgat ggattttagt agtaagatag ccataaataa gggctggtca
     6781 gatgataaaa aatattgtgt gacagatcaa aatcagtaaa aatatttctt gcgtgtttct
     6841 gataagtcga agttagatta taaaaaaatt tgaatttgat atgatggaga aagtagcttc
     6901 tcttggagtt ccgatgtgta aaccaattag cattgaactc tgcgatgacg aagtacattc
     6961 tttacacgaa tggatagacg gaagagatgc aatagatagc attttagctt attcagaaaa
     7021 ccaacaatac acatatggtg tagaagcagg aaagatactt agaaagattc atacaattcc
     7081 tgctacagaa gtttgtgaag attgggaaat ttttttaatc taaaaattga tgataaaatc
     7141 tccaacgaaa tgatatggta agtccgaaac gctcagtgtg taagcttgtt gctaaaagcg
     7201 taaaaacctt ggaacgaaag gaataataga cttcctgcga aacaaaaata caatacaata
     7261 aaactatgga tgatgaagca agtaaacaat taagcgatag ccgtttcaag atccttgtag
     7321 gtgttcagcg cacgactttt gaagagatgt tagctgtgtt aaaaacagct tatcaacgta
     7381 aacgcgcaaa aggtggacga aaaagcaaat taagcctaga cgatctcctt atggtaacta
     7441 ttcaatacat gcgagaatag agcacttatg aacaaattgc ggctgatttt ggcattcacg
     7501 aaagcaactt aatccgtcgg agtcaatggg ttgaagcaac tcttattcaa aatggtttta
     7561 cgatttcaaa ttctgcctta attctgtaaa aacagtaaaa ttcgaaggat tgtaaggtaa
     7621 gagttttttt ctttctgaaa aaatggtata atagcaatca aaactagaaa ataaaacgga
     7681 atttggaaca gatttgtctg tatcctagta gagtggtgat actatgaaga ttagtaagag
     7741 gcacttatta aattattcca tcttgattcc ctacttgctt ttatctattt tgggcttgat
     7801 tgtggtctat tcgaccacca gtgctatttt aattgaagaa ggcaagagcg ccttgcagtt
     7861 ggttcgaaac caaggaatct tttggattgt tagtttgata ctgattgcct taatttataa
     7921 attgagacta gattttttga gaaatgagcg actaatcatt ttagttatat taatagaaat
     7981 gcttttattg ttcttggctc gttttattgg tatttcagta aacggggcat acggttggat
     8041 ttcggttgca ggagtaacta ttcagccagc tgagtactta aaaatcatta ttatttggta
     8101 tttagctcac cgattctcca aacagcaaga agaaatagct acttatgatt ttcaagtttt
     8161 gactcaaaat caatggcttc cccgtgcttt taatgattgg cgattcgttc tcctagttct
     8221 gattggaagt ttgggaattt tccctgattt aggaaatgcg actattttag tcttggtttc
     8281 cttgattatg tatacagtta gtggaatcgc ttatcgctgg ttttcaacca ttctggcgct
     8341 cgtatctgcc acttctgtct ttgtcttgac cactatcagc ctaatcggtg ttgagacctt
     8401 ttcaaaaatt ccagtatttg gctatgtagc caagcgcttt agtgcctttt ttaatccttt
     8461 tgccgatcgt gctgatgcag gtcaccagtt agctaattct tattttgcca tggtcaatgg
     8521 tggttggttt ggtctaggtc ttggaaactc gattgaaaaa cgaggttatt tgccagaagc
     8581 tcatacagac tttgtctttt ctatcgtgat tgaagaattt ggctttgttg gtgccagtct
     8641 tattttagct ctcttgtttt tcatgatttt gcggattatc ttggtcggta tccgagcgga
     8701 gaatcctttc aatgccatgg ttgcactcgg tgtcggaggg atgatgttgg ttcaggtatt
     8761 tgtcaatatc ggagggattt cgggcttgat tccatctaca ggagtgactt tccctttctt
     8821 atcccagggt ggaaatagtc ttctagtctt atcagtggca gtagcctttg tcttaaatat
     8881 tgatgccagt gaaaaacgcg ctaagttgta ccgagaattg gaaaatcaac caatgaacct
     8941 tctgttgaag taggataaag aaaggatagt ttatgtctct tcaaaaatta gaaaattata
     9001 gtaataaaag tgttgtgcaa gaagaagtct tgattctaac agaattactg gaagatatta
     9061 ctaaaaatat gcttgcccca gagacctttg aaaaaataat acagttgaaa gaattatcaa
     9121 cgcaggaaga ttatcaaggt ctaaaccgtc tagtgactag cttatcaaat gatgaaatgg
     9181 tctatatttc acgctatttc tctatcttac ctcttttgat taatatttca gaggatgtgg
     9241 atttagctta tgaaatcaat catcaaaata atattgatca ggactattta ggtaaattat
     9301 ctacaacgat taaattggta gcagaaaagg aaaatgccgt tgagatccta gaacacttga
     9361 atgttgtccc tgttttgaca gcccatccaa cacaagtgca acgcaaaagt atgttggatt
     9421 taacaaatca tattcatagt cttttgcgta aataccgtga tgttaagttg gggttgatca
     9481 ataaagataa atggtacaat gatttgcgtc gttacatcga aattatcatg cagacagaca
     9541 tgattcgtga gaaaaaatta aaagtgacta acgaaatcac gaatgctatg gaatattata
     9601 acagctcctt tttgaaagct gtacctcatt tgacgacgga gtataagcgc ttagcgcaag
     9661 cgcatggtct gaatttaaaa caggctaaac caatcaccat gggtatgtgg ataggtggtg
     9721 accgtgatgg aaatccattt gttacagcaa agaccttgaa gcagtctgca ctcactcagt
     9781 gtgaagtcat catgaactac tatgataaaa agatttacca actttatcgt gaattttctc
     9841 tttcaactag cattgtcaac gtcagcaagc aagtcagaga aatggctcgt caatccaagg
     9901 ataactcgat ttaccgcgaa aaagagcttt accgtcgtgc cttgtttgat attcaatcaa
     9961 aaattcaggc aactaaaacc tatctgattg aggatgaaga agttgggact cgttatgaaa
    10021 ccgccaatga tttctacaag gatttgattg ccattcgaga ttctctacta gaaaataagg
    10081 gcgagtcctt gatttcaggt gattttgtgg aattattgca ggcagtagag atatttggtt
    10141 tttacttagc atcaattgat atgcgacaag actctagcgt ctatgaagcc tgtgtggcag
    10201 aactcttgaa atcagcagga attcattctc gttatagcga gttgagcgaa gaagaaaagt
    10261 gtgaccttct cttgaaagaa ttagaagaag atccccgaat tctttctgcg actcacgcag
    10321 aaaaatcaga attattagca aaagaattag ctatttttaa gacggctcgt gttttgaaag
    10381 ataagttggg agatgatgtc atccgtcaga ccatcatttc acatgcaacc agcctttctg
    10441 atatgctaga attagctatt ctgttaaaag aagtaggact ggtggatacg gaaagggcgc
    10501 gtgttcagat tgttcccctt tttgaaacaa ttgaagactt ggatcattca gaggaaacaa
    10561 tgagaaaata tctttctctt agccttgcca aaaaatggat tgactcacga aataactacc
    10621 aagaaatcat gcttggctac tctgacagta ataaagatgg cggttacttg tcatcatgtt
    10681 ggaccctcta caaggctcaa caacaattga ctgctattgg agatgaattt ggcgttaagg
    10741 ttaccttctt ccatggtcgt ggtggtactg tcggtcgtgg tggtgggcca acctatgaag
    10801 ccattacatc tcaaccgctc aagtctatca aggatcgtat ccgcttgacg gagcagggtg
    10861 aagtaattgg gaataaatac ggtaacaaag acgccgctta ctataacctt gaaatgctag
    10921 tatcggcagc tattaaccgt atgattactc agaagaagag cgataccaat accccaaatc
    10981 gttatgaagc cattatggat caagtagtgg accgtagtta cgatatctac cgtgatttgg
    11041 tctttggtaa tgagcatttc tatgattatt tcttcgagtc aagtccaatc aaggctattt
    11101 caagttttaa tattggttct cgtccagccg ctcgtaagac tattactgaa atcggtggtt
    11161 tgcgtgccat cccttgggta ttctcatggt cacagagtcg tgttatgttc cctggatggt
    11221 acggggttgg ttcaagcttc aaggaattta tcaataaaaa tccagagaat attgctatct
    11281 tacgagatat gtaccaaaat tggcctttct tccaatcgct tctttcaaat gttgatatgg
    11341 ttttgtcaaa atcaaatatg aatattgctt ttgaatatgc taaactttgt gaagacgagc
    11401 aagttaaggc catctatgag actattttaa atgaatggca agttactaag aacgttatct
    11461 tggctattga aggacatgac gaactcttag ctgacaatcc atatctaaaa gctagtctgg
    11521 attaccgtat gccttacttt aatattctca actatattca gttagagttg attaaacgcc
    11581 aacgtcgtgg agaattgtcc agtgatcaag aacgattgat tcatatcacc atcaacggaa
    11641 ttgcgacagg attgcgtaat tcaggttgat aattttcaag agtgaatact aaaagtgaat
    11701 atcaaaaaaa ttctaataga ctattgacaa gtaatttaaa aatgatataa tttaaccatt
    11761 cagaaaagta atcatacaaa ctttttagag agtctgtggt agctgaaaac agataagtgg
    11821 cgatgatgaa aattgggctg aatgctattt agaatttgaa attataaaaa ttcggtaagc
    11881 acaccttaca gtgcatctcg ttattgcgag actgagcgat agggaaattc cctataattg
    11941 aggtggtacc gcgcatcgac gtcctcacac aagttttttg tgtgaggatt tttttgatgg
    12001 aggttagtat ggaaagaaaa cgatggcgtc gcttgtttag ataagtgaaa tatgttaaag
    12061 gaaataaaaa ggagaaacag aatgaaaaat aaacgtttaa ttggaattat tgctgcatta
    12121 gcagtcttag tagcaggaag cttgatttat tcttcaatga ataaatcaga agctcagaat
    12181 aataaggatg agaagaaaat aaccaagatt ggtgtgcttc aatttgtgag ccatccatcc
    12241 cttgatttga tttataaagg gatccaagat ggacttgcag aagaaggata taaagatgat
    12301 caagttaaaa ttgattttat gaactcagaa ggtgaccaaa gtaaggttgc gacaatgagt
    12361 aaacaattgg ttgcaaatgg gaatgacctt gtggttggta tcgcaacacc agcagcccaa
    12421 gggttggcta gtgcaacaaa agacttaccg gttatcatgg ccgctattac agacccaatt
    12481 ggtgctaact tggttaaaga tttgaaaaaa ccaggtggca acgttacagg ggtatctgac
    12541 cacaatccag ctcaacaaca agttgaactc atcaaggctc tgacaccgaa tgtgaaaaca
    12601 atcggagctc tttactcaag tagcgaagac aattcaaaaa cacaggtcga agaatttaag
    12661 gcttatgctg aaaaagcagg tctgacagtg gaaacatttg cagttccttc aacaaatgaa
    12721 attgcctcaa ctgtcactgt tatgactagc aaggtagatg ctatttgggt tccaattgat
    12781 aacaccattg catcaggatt tccaacggtt gtctctagca atcaaagttc taagaaacca
    12841 atttatccca gtgcgacagc tatggtagaa gtaggtggtt tggcatcagt tgtaattgac
    12901 caacatgacc ttggtgtggc aacaggtaaa atgattgtgc aagtcttgaa aggtgcaaaa
    12961 ccagccgata ccccagtcaa tgtcttttca actggtaagt cagtcatcaa taaaaaaata
    13021 gcacaagaac taggtattac tattcctgag tctgttctca aagaagcagg acaagtcatc
    13081 gaataaataa ggtagggagg aggagactct tcccattttt taaaaagatg aatatagaaa
    13141 ggattaaata aaatatgatt gtttccatta tttctcaagg atttgtctgg gctattctag
    13201 gtctgggaat ctttatgaca tttaggattt taaactttcc agatatgacg acagaaggtt
    13261 ccttccctct tgggggagct gttgctgtca ctttgataac caaaggcgtg aacccatttt
    13321 tagcgacact tgttgctgta ggagcaggtt gtttggctgg aatggcagca ggccttcttt
    13381 atacaaaagg gaagatccca accttgctct cagggatttt ggtgatgact tcttgtcact
    13441 caatcatgct cttgattatg ggacgtgcga atttaggcct gcttggaacc aagcaaattc
    13501 aggatgtttt gccttttgat tcggatttga atcaactctt gacaggtctc atctttgtga
    13561 gtattgttat tgctctcatg ctctttttct tggacactaa actcggacaa gcctatattg
    13621 ctacagggga taatcctgat atggctagaa gtttcgggat tcatactgga cgcatggagc
    13681 tcatgggctt ggtcttatca aatggtgtga ttgcccttgc aggtgccctc attgctcagc
    13741 aagaaggcta tgccgatgtg tctcgaggga tcggggttat cgttgtgggg cttgcaagtt
    13801 tgattattgg agaagttatt ttcaagagtt tgagcttggc agagcgtttg gttactatcg
    13861 ttgtaggttc tatcgcttat caatttttag tgtgggcagt tatcgcactt ggctttaata
    13921 caagttacct tcgtttatac agtgccttga ttttagcagt ctgcctcatg attccaacat
    13981 ttaagcaaac aatcttgaaa ggagccaagt taagcaaatg acagcaattg tagaattaaa
    14041 aaatgcaacc aaaatcgtta aaaatggctt tgatgaagaa aagattattt taaatgatgt
    14101 ttccttagaa atttttgaac gggactttat cacgattttg ggcggaaatg gtgctggaaa
    14161 atcaactctc tttaacacta tagcagggac cttatcacta actagtggaa ctatccgtat
    14221 tttaggtgaa gatctcacta agttttcacc cgagaagcgt gccaagtacc tgtctcgtgt
    14281 cttccaagat ccaaagatgg ggacagctcc ccgtatgacg gtcgctgaaa atcttttaat
    14341 cgccaagttt cgtggtgaaa agcgtggatt gttaccaaga cgcttgacta gctataagga
    14401 tgaatttcag gcaaccattg aaaaagtagg aaatggtctt gagaaacact tgaatacacc
    14461 gattgagttc ttatcaggtg gacaaagaca ggctttgagt ctcttgatgg caaccttgaa
    14521 gcgacctgaa ttactcctgt tagatgagca tactgctgcc ctggatccaa agactagtgt
    14581 tgctttgatg gaattgacag atgaatttgt taagaaagat cagctaacag cccttatgat
    14641 tactcatcat atggaagatg ctctcaaata cggcaatcgc ttgattgtca tgaaagaagg
    14701 acggattatc caagatttaa accaagaaga aaaagcaaaa atgaaaatct ctgattatta
    14761 tcaactcttt gaataaagta gggaaaatga gtgaaatggt ttactttttt tctgaaataa
    14821 agtatactat ataaagtaaa ctatgataac atggaggtat tgtgtatggt tgacaaacaa
    14881 gtcattgaag aaatcaaaaa caatgccaac attgtggaag tcataggaga tgtgatttct
    14941 ttacaaaagg caggacggaa ctatctaggg ctctgtcctt ttcatggtga aaaaacacct
    15001 tctttcaacg ttgtagagga caagcagttt taccactgtt ttggttgtgg tcgctcaggt
    15061 gatgtcttta aattcatcga ggagtaccaa ggggttacct ttatggaggc tgtccaaatc
    15121 ttaggtcagc gtgtcgggat tgaggttgaa aaaccgcttt atagtgaaca gaagccagcc
    15181 tcgcctcacc aagctcttta tgatatgcac gaagatgcgg ctaaatttta ccatgctatt
    15241 ctcatgacaa cgactatggg cgaagaggcc agaaattacc tttatcagcg gggtttgaca
    15301 gatgaagtgc ttaaacattt ttggattggt ttagcacctc cagaacgaaa ctatctctat
    15361 caacgtttgt ctgatcagta tcgtgaagaa gatttactgg attcaggcct gttttatctc
    15421 tcggatgcca atcaatttgt agacaccttt cacaatcgca ttatgtttcc cctgacaaat
    15481 gaccaaggaa aggtcattgc cttctcaggt cgtatctggc aaaaaacaga ttcacaaact
    15541 tctaagtata aaaacagccg atcgactgca atttttaaca aaagttacga attatatcat
    15601 atggataggg caaaaaaatc ttctggaaaa actagtgaga tttacctgat ggaaggattc
    15661 atggatgtta ttgcagccta tcaggctgga atcgaaaatg ctgtggcgtc gatgggaacg
    15721 gccttgagtc gagagcatgt tgagcatctg aaaaggttaa ccaagaaatt ggttcttgtt
    15781 tacgatggag ataaggctgg gcaagccgcg atattgaaag cattggatga aattggtgat
    15841 atgcctgtgc aaatcgttag catgcctgat aacttggatc ctgatgagta tctacaaaaa
    15901 aatggtccag aagacttggc ctatctatta acgaaaactc gtattagtcc gattgagttc
    15961 tacattcatc agtacaaacc tgaaaacagt gaaaatctgc aggctcagat tgagtttatt
    16021 gaaaaaatag ctcccttgat tgttcaagaa aagtccatcg ctgctcaaaa cagctatatt
    16081 catattttag ctgacagtct ggcgtccttt gattatgccc agattgagca gattgttaat
    16141 gagagtcgtc aggtgcaaag gcagaatcgc atggaaggaa tttccagacc gacgccaatc
    16201 accatgccta tcaccaagca gttatcggct attatgaggg cagaagccca tctactctat
    16261 cggatgatgg aatcccctct tgttttgaac gattaccgtt tgcgagaaga ctttgcattt
    16321 gctacacctg aatttcaggt cttatatgac ttgcttggcc agtatggaaa tcttcctcca
    16381 gaagttttag cagagcagac agaggaagtt gaaagagctt ggtaccaagt tttagctcag
    16441 gatttgcctg ctgagatatc gccgcaggaa cttagtgaag tagagatgac tcgaaacaag
    16501 gctctcttga atcaggacaa tatgagaatc aaaaagaagg tgcaggaagc tagccatgta
    16561 ggagatacag atacagccct agaagaattt gaacgtttaa tttcccaaaa gagaagaatg
    16621 gagtaataat ggcaacaaaa caaaaagaag taacaacatt tgacgtacag gtagcagaat
    16681 ttatccgtaa tcataagcaa aaagggacag caacagatga tgaaatcaat gcaagtctgg
    16741 ttattccttt taccttggac gctgatggga ttgaagatct cttgcaacgg attcaggatg
    16801 caggcatttc tatcacagat aacgaaggaa atccaagtgc gcgtgttctt agcaatgaag
    16861 aagaaccaga actcagcgat gaggacttga ttgggtcaac ttctgctaag gtcaatgacc
    16921 ctgtccgtat gtacttgaaa gaaatagggg tcgttcctct cttgaccaat gaagaggaga
    16981 aagagttggc actggctgtt gaagctggcg atatcgaagc caaacaacgt cttgcggaag
    17041 ccaatcttcg tttggttgtt tctattgcca aacgctatgt cggtcgtggc atgcagttcc
    17101 ttgacttgat tcaagaagga aatatgggct tgatgaaggc ggttgacaag tttgactatt
    17161 ctaaagggtt caagttttca acttatgcaa cttggtggat tcgtcaggct atcactcgtg
    17221 ctattgcgga tcaagctcgt accatccgta tcccagttca catggttgaa actatcaata
    17281 aattggttcg tgaacagcgg aatctccttc aagaattggg gcaagatccg acaccagaac
    17341 agattgctga acgaatggat atgacacctg ataaggttcg tgaaatcttg aagattgccc
    17401 aagaaccagt atctcttgaa actcctatcg gtgaagagga cgatagccac cttggagact
    17461 ttatcgaaga tgaagtgatt gaaaatccag tggattatac gactcgtatc gtcttgcgtg
    17521 agcaattgga tgaaatctta gatactctta cagaccgtga agaaaatgtt ctgcgtctac
    17581 gttttggact agatgatgga aaaatgcgca cacttgaaga tgtggggaaa gtctttaacg
    17641 taactcgtga gcgtatccgt cagattgaag caaaggcttt gagaaaacta cgccaaccaa
    17701 gccgcagcaa accgcttcgt gattttattg aagactaaga gtgaggaaaa tatggcttat
    17761 acagaagagc aaattgaaaa catcaaaaca cggattttaa cagccttgga agaagtcatc
    17821 gaccctgagt tgggaatcga tattgtcaat cttggtttga tctatgagat tcgttttgac
    17881 ggtgacacag ggcaaacaga gattgacatg actttgacaa ctatgggttg tcccttagca
    17941 gaccttttga cagaccagat ttatgatgct atgatagagg ttccagaagt aacggatact
    18001 gaggttaaat tagtctggta tccagcctgg acagttgaaa aaatgagtcg ctatgcccgc
    18061 attgccctag gcattaagta aacatataaa agcatgtgag agactgttgg aaaagcgagg
    18121 aaatttcccc tcttttcctc tagtctctcc tttcttttgc tgattttatt caaagaaaat
    18181 gatataatag tagttatgga gaaaaagaaa ttacgcatca atatgttgag ttcaagtgag
    18241 aaagtagcag gacagggagt ttcaggtgct taccgtgaat tagttcgtct tcttcaccgt
    18301 gctgccaagg accaattgat tgttacagaa aatcttccaa tcgaggcaga tgtgactcac
    18361 tttcatacga ttgattttcc ctattattta tcaaccttcc aaaagaaacg ctcagggaga
    18421 aagattggct atgtgcattt cttgccagct acacttgaag gaagtttgaa aattccattt
    18481 ttcttaaagg gaattgtgaa acgctatgta ttttcttttt acaaccggat ggagcacttg
    18541 gttgtggtca atcctatgtt tattgaggat ttggtagcag ctggtattcc acgtgaaaaa
    18601 gtgacctata ttcctaactt tgtcaacaag gaaaaatggc atcctctacc acaagaagag
    18661 gtagtcagac tgcgcacaga tcttggtctt agtgacaatc agtttatcgt agtaggtgct
    18721 gggcaagttc agaaacgtaa agggattgat gactttatcc gtctggctga ggaattgcct
    18781 cagattacct ttatctgggc tggtggcttc tcttttggtg gtatgacaga tggttatgaa
    18841 cactataaga aaattatgga aaatccccct aaaaatttga tttttccagg cattgtatcg
    18901 ccagagcgga tgcgcgaatt gtatgctcta gcggatcttt tcttgttgcc tagttacaat
    18961 gagctctttc ctatgactat tttagaagct gcgagttgtg aggctcctat tatgttgcgt
    19021 gatttagatc tctataaggt gattttggag ggaaattatc gggcgacagc gggtagagaa
    19081 gagatgaaag aggctatttt ggaatatcaa gcaaatcctg ctgtcttaaa agatctcaaa
    19141 gaaaaggcta agaatatttc cagagagtat tctgaagagc atctgttaca aatctggttg
    19201 gacttttatg agaaacaagc cgctttaggg agaaagtaaa aagtgaggta atctatgcga
    19261 attggtttat ttacagatac ctattttcct caggtttctg gtgttgcgac cagtattcga
    19321 accttgaaaa cagaacttga aaagcaggga catgctgttt ttatctttac gacgacagat
    19381 aaggatgtca atcgctacga agattggcaa attatccgca ttccaagtgt tcctttcttt
    19441 gcttttaagg atcgtcgctt tgcctaccga ggttttagca aggcacttga aattgctaaa
    19501 cagtatcagc tagatattat ccatactcag acagaatttt ctcttggcct gttggggatt
    19561 tggattgcgc gtgaattgaa aattccagtc atccatacct atcacaccca gtatgaagac
    19621 tatgtccatt atattgctaa ggggatgttg atccggccga gtatggtcaa gtatctggtt
    19681 agaggtttcc tgcatgatgt ggatggggtt atttgcccta gtgagattgt ccgtgacttg
    19741 ctatctgatt ataaggtcaa ggttgaaaaa cggatcattc ctactgggat tgaattagcc
    19801 aagtttgagc gtccggaaat caagcaggaa aatttgaaag aactgcgtag taaactaggg
    19861 attcaagatg gtgaaaagac gttgcttagt ctttcgagaa tctcctatga aaaaaatatt
    19921 caagcagttt tagcagcctt tgctgatgtt ctgaaagagg aagacaaggt taaactggta
    19981 gtagctgggg atggccctta tctgaatgac ctcaaagagc aagcccagaa cctagagatt
    20041 caagactcag tcatctttac agggatgatt gctcctagtg agacggctct ttactataaa
    20101 gcggcggatt tcttcatttc ggcatcgaca agcgaaacgc aaggtttgac ctacttggaa
    20161 agcttagcca gtggaacacc tgtcattgct cacggaaatc cttatttgaa caacctcatc
    20221 agtgataaaa tgtttggaac cttgtactat ggagaacatg atttggctgg tgctattttg
    20281 gaagccctga ttgcaacacc agacatgaac gagcatacct tatcagagaa attgtatgag
    20341 atttcagctg agaactttgg gaaacgagtg catgagtttt atctggatgc cattatttca
    20401 aataacttcc agaaagattt ggctaaagat gatacggtca gtcagcgtat ctttaagaca
    20461 gttttgtatc ttcagcaaca ggtggttgct gtacctgtaa aaggatctag acgcatgttg
    20521 aaggcttcaa aaacacagtt gatcagtatg agagactatt ggaaagacca tgaagaatag
    20581 aaagaggaac agctatgaaa aaaacaatta atgagaagcg gtcgtgataa aaagattgcg
    20641 ggtgtttgtg ctggggtggc ccattatctg gatatggatc cgactatcgt tcaagtcatt
    20701 tggggtgttc ttacttgctg ttacggagct ggaattgtag cttacattat tttatggatt
    20761 atcgcaccag tagcaactga ctattgaaaa ctagctcaat tcatgctaag ataatagaaa
    20821 atagaatgta acgaaggaga gaaaaatggc atttggagat aatggaaatc gtaaaaaaac
    20881 tatgtttgag aaaataacct tgtttatcgt gattatcatg ctagtagcaa gtttattggg
    20941 aatttttgca actgcaattg gtgccctcag taatctataa aatagattca agaaaattta
    21001 gtgactggga tttcccagcc cttttttaaa gtgagaagaa ataatgagta tgtttttaga
    21061 tacagctaag attaaggtca aggctggtaa tggtggcgat ggtatggttg cctttcgtcg
    21121 tgaaaaatat gtccctaatg gaggcccttg gggtggtgat ggtggtcgtg gaggcaatgt
    21181 ggtcttcgtt gtagacgaag gactacgtac cttgatggat ttccgctaca gtcgtcattt
    21241 caaggctgat tctggtgaaa aagggatgac caaagggatg catggtcgtg gtgctgagga
    21301 ccttagagtt cgagtaccac aaggtacgac tgttcgtgat gcggagactg gcaaggtttt
    21361 aacagatttg attgaacatg ggcaagaatt tatcgttgcc cacggtggtc gtggtggacg
    21421 tggaaatatt cgtttcgcga caccaaaaaa tcctgcaccg gaaatctctg aaaatggaga
    21481 accaggtcag gaacgtgagt tacaattgga actaaaaatc ttggcagatg tcggtttagt
    21541 aggattccca tctgtaggga agtcaacact tttaagtgtt attacctcag ctaagcctaa
    21601 aattggtgcc taccacttta ccactattgt accaaattta ggtatggttc gcacccaatc
    21661 aggtgaatcc tttgcagtag ccgacttgcc aggtttgatt gaaggggcta gtcaaggtgt
    21721 tggtttggga actcagttcc tccgtcacat cgagcgtaca cgtgttatcc ttcacatcat
    21781 tgatatgtca gctagcgagg gccgtgatcc atatgaggac tacctagcta tcaataaaga
    21841 gctggagtct tacaatcttc gcctcatgga gcgtccacag attattgtag ctaataagat
    21901 ggacatgcct gagagtcagg aaaatcttga agactttaag aaaaaattgt ctgaaaatta
    21961 tgatgaattt gaagagttac cggctatctt cccaatttct ggattgacca agcaaggtct
    22021 ggcaacactt ttagatgcta cagctgaatt gttagacaag acaccagaat ttttgctcta
    22081 cgacgagtcc gatatggaag aagaagctta ctatggattt gacgaagaag aaaaagcctt
    22141 tgaaattagt cgtgatgacg atgcgacatg ggtactttct ggtgaaaaac tcatgaaact
    22201 ctttaatatg accaactttg atcgtgatga atctgtcatg aaatttgccc gtcagcttcg
    22261 tggtatgggg gttgatgaag cccttcgtgc gcgtggagct aaagatgggg atttggtccg
    22321 cattggtaaa tttgagtttg aatttgtaga ctaggagact ggtatgggag ataaaccgat
    22381 atctttccga gatgcggatg gtaattttgt ttccgccgca gacgtttgga atgaaaagaa
    22441 attggaagaa ctatttaatc gtctcaatcc aaatcgtgcc ttgagattgg cacgaactaa
    22501 aaaggaaaat ccatctcagt aaagaagcta aaaaatcccg tgcctcatca gacacgggat
    22561 tttgtggtac gacgggcatg tatagcgaac tgaatctgga atagcacagc atatcttcta
    22621 aaatatagta aaatgaaatg agaacaggac aaatcgattt ctaacaatgt tttagaagta
    22681 gaggtg
//
LOCUS       DAVLTM010000020        22338 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLTM010000020 DAVLTM010000000
VERSION     DAVLTM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.020"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..204)
                     /locus_tag="VYN16_000689"
     CDS             complement(<1..204)
                     /locus_tag="VYN16_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403257.1"
                     /translation="MLIVLISVIVALLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIEISGANLLLSFLKKLQ"
     gene            complement(212..583)
                     /locus_tag="VYN16_000690"
     CDS             complement(212..583)
                     /locus_tag="VYN16_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV1403258.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            1220..2083
                     /locus_tag="VYN16_000691"
     CDS             1220..2083
                     /locus_tag="VYN16_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403259.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKIHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(2269..2739)
                     /locus_tag="VYN16_000692"
                     /pseudo
     CDS             complement(2269..2739)
                     /locus_tag="VYN16_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(2705..3511)
                     /locus_tag="VYN16_000693"
     CDS             complement(2705..3511)
                     /locus_tag="VYN16_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1403260.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3718..4359)
                     /locus_tag="VYN16_000694"
     CDS             complement(3718..4359)
                     /locus_tag="VYN16_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403261.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(4356..6464)
                     /locus_tag="VYN16_000695"
     CDS             complement(4356..6464)
                     /locus_tag="VYN16_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1403262.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATVLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6516..6803)
                     /locus_tag="VYN16_000696"
     CDS             complement(6516..6803)
                     /locus_tag="VYN16_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1403263.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTVMEYFYGFE"
     gene            7234..7554
                     /locus_tag="VYN16_000697"
     CDS             7234..7554
                     /locus_tag="VYN16_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1403264.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVCIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(7607..8179)
                     /locus_tag="VYN16_000698"
     CDS             complement(7607..8179)
                     /locus_tag="VYN16_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1403265.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            8421..9092
                     /gene="sdaAB"
                     /locus_tag="VYN16_000699"
     CDS             8421..9092
                     /gene="sdaAB"
                     /locus_tag="VYN16_000699"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1403266.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            9101..9973
                     /gene="sdaAA"
                     /locus_tag="VYN16_000700"
     CDS             9101..9973
                     /gene="sdaAA"
                     /locus_tag="VYN16_000700"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1403267.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9995..10627
                     /locus_tag="VYN16_000701"
     CDS             9995..10627
                     /locus_tag="VYN16_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1403268.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            10677..10754
                     /locus_tag="VYN16_000702"
                     /pseudo
     CDS             10677..10754
                     /locus_tag="VYN16_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10975..12825)
                     /locus_tag="VYN16_000703"
     CDS             complement(10975..12825)
                     /locus_tag="VYN16_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1403269.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(12867..14024)
                     /locus_tag="VYN16_000704"
     CDS             complement(12867..14024)
                     /locus_tag="VYN16_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1403270.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            14082..15248
                     /locus_tag="VYN16_000705"
     CDS             14082..15248
                     /locus_tag="VYN16_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1403271.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            15387..15713
                     /locus_tag="VYN16_000706"
     CDS             15387..15713
                     /locus_tag="VYN16_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1403272.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            15700..16293
                     /locus_tag="VYN16_000707"
     CDS             15700..16293
                     /locus_tag="VYN16_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1403273.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            16286..17197
                     /locus_tag="VYN16_000708"
     CDS             16286..17197
                     /locus_tag="VYN16_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1403274.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKENQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            17259..18323
                     /locus_tag="VYN16_000709"
     CDS             17259..18323
                     /locus_tag="VYN16_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403275.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(18394..19240)
                     /locus_tag="VYN16_000710"
                     /pseudo
     CDS             complement(18394..19240)
                     /locus_tag="VYN16_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
     gene            complement(19515..20501)
                     /locus_tag="VYN16_000711"
     CDS             complement(19515..20501)
                     /locus_tag="VYN16_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1403276.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(20773..22248)
                     /locus_tag="VYN16_000712"
     CDS             complement(20773..22248)
                     /locus_tag="VYN16_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1403277.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
BASE COUNT         6757 a         4868 c         3914 g         6799 t
ORIGIN      
        1 ctgtaatttt tttaaaaaac ttaatagtaa atttgctcca ctgatttcta tcaatatttc
       61 aattggtaaa aaatagtaat tccaaataaa atgagaaatc agcaatgata gaaaaataac
      121 aatatcaatt ttcctgttgt tgacatactc tgtaatttta tgatacagta atgcaacaat
      181 aactgaaatt aaaacaatca acataagatt attaacctat tctcaattga ttatcacgtc
      241 gcccatccca gccatcaata atattggtaa aaatccaatc agctccagca ccaattccag
      301 cagatgcaat acttctaata acagtatttg caaaagcatt tccaatatga attcctaata
      361 ctcctaaacc tttaacaatc ccagctacca gtgcggagcg taatccataa gatgcaagaa
      421 ctagttgaag gcctccacct cctgctgctg ctaatgctgc atatgctgca ccagtaatgg
      481 ctcctgtaat tgcccacctg ggtatatata tagccccccc atcaaggtac atcatctctt
      541 cttcatcaat cacaacataa ttatttggta atacaagttc catttatttt ctcctttttt
      601 attaagcatg cttaggatgg tttcctaaca agtgatcgca ggctttgttt agttacctaa
      661 attagacttc cttcctttat cttattttaa atatttaaaa ccagttactg cttgaagctt
      721 aacagtttac ttaattcaaa taacagctaa tcactgattc cctgatttta tcaaatatac
      781 taataacaac aattgtgata cgatcatcat tataagaaca acatttaacg aatcaaagtt
      841 tttccgagat aataataaag caacagcaag cagtattatt actaaagatt gaataactac
      901 tattactctt aacattttat cctccactaa caactcaagc tcccttacat tatatcgacc
      961 agccccatgg aaagattata gctagactaa tcaatgatat aatacatact agaatacaaa
     1021 atattttttt attcatactt tttctcctat ctttattttc tacctacata gtacctctta
     1081 ttaagctcat ttcaaaatta cgttaacttt tttcacaaat ttatttcgac tttctttttt
     1141 actttttaca ttgctatgat acaatatttt taacaagatt ttattatttg ttaacttttt
     1201 tcacagaaag gagaaaatca tgcgttatga tttcggaaag gtctataaag aaatacgtga
     1261 gtcaaagggg ctgacccaag aagaggtctg tgggggtgtt ctctcaagaa ccagcttatc
     1321 aaaaatcgaa agtggcaaga caaccccaaa atatgaaaat atggaatttc ttctccgtca
     1381 aatcaatatg agttttgaag agtttgaata tatctgtcaa ctttatcaac caagtcaacg
     1441 cacagagatt atgcaaacct atctcaatat gcgttccatt atcgggacca gcgatcttgt
     1501 caatttattt caaaaatgcc aagactacct caagatccac cacgatctcc ctatagaaga
     1561 aatcagagat atgcttgaag ttgtcattta tatccgtcaa cacggggcag gggaactatc
     1621 agatcacgca gaacaggttg tcaaaaaact ttggagaaaa attgaaaaac aagatacatg
     1681 gtatgagagt gatctaaaaa tcctcaatac cattcttttc agttttccca ttgaatacct
     1741 ccatctcatc actggaaaaa tattgcaacg cctagaagtc tataaaaact atcaacattt
     1801 atatgacttg cgaatagcaa tcctgctcaa cctttccaca ctctatctat acaatcaaga
     1861 taaaaacatg tgtaagcaaa tctgctacac tttactggag gacgccaaga ataagaaaag
     1921 ctacgatagg ctggctatct gctatgtccg cattgggatt tgtacagacg attctaaact
     1981 tatccaaaaa ggtttctcac tcctagagct gaccgaggaa acttctatgc tgtctcatct
     2041 caaaaaagaa gtagagatct attaccaagc gaaggaaaga taaaaaagcc gagggagctc
     2101 ctcgaccttt tcatagaatc gccgaacgat ttaacgagaa agtatgactt ttacgtttat
     2161 cccaactcaa ttatgacatt tttttcaaaa gtcaatatat ctcacttttt caacgacaag
     2221 aaagaggctg ccaatcgacc aacctcttat tctgaaccca tcactccatc actttttagc
     2281 ttcattcgct ttcttagcga ctgcaatctg gtattcgact tggtcaaacc ccttaccggt
     2341 acaaccatga gcaattgtag tcgctcctat ctgatgcgct atttcaacca attttttaga
     2401 aatcagaggg cggctcaagg cagataccaa gggatacttt tgttcataat aggcatgtga
     2461 ctaatgagcc actagcacat aatctgtagc aaattcgtcc ttaacatcaa tgacataaga
     2521 ttctactgcc caaaccttaa gagccttatc atggatgaaa tctaagtctt tcccttcacc
     2581 cacatccata caaactgaaa caacatcata gtctttcttt aaccaggtaa tagcaactga
     2641 tgtgtccaaa ccgccagaat aggccaaaat tactttttct ttactcatgt tcttttcctt
     2701 ctttctattt ttcaatggat gctttaaagg catcctcaat gagtttgtct aattccccag
     2761 actccttcaa cttttgaatg gttttatcga ctgcctcttt caattccttg ctatcttttt
     2821 tcatggctac cgcataggaa tcatcttgct ctttttcaaa attgaggtct gcgattgcta
     2881 aatcaggatt attttccaca aatcccttgg caacgggttc ttcaaaaata acggcatcca
     2941 cttgccctga ttttaaatct gtgattaaat tcccattttt aggcagagat acgagggaag
     3001 aattttgtag caaatctttc gccatcgtct cttgaatcga acctttctgc gctccgacct
     3061 ttttctgcgc caaatcgttg acagactgat aagtggctaa atcagatttt ttgacaatga
     3121 gtttattttt tgcagtatag tagggagttg aaaaatcaaa caccttgctc cgttcatctg
     3181 tcttagaaac acctgatatg gcaaggtcag cttttcctga ttgaacgcta gccagtacat
     3241 tgtcaaaact cataggagat agttccaatt cgacacctag ttctgttgcg atagccttgg
     3301 ctaattcgat atctgaaccc acaatctgat ttttcccatc aaccactttt tgatattcaa
     3361 atggagcaaa atctggattg agggctacaa ccaattttcc tttggacttg atggcttcaa
     3421 ttccagccga ttgattgtta ctacaagcaa atagtagggg gagcatcact aaaccaaaca
     3481 tcgtcatcaa caccttcttc atcttattca taacaaagcc tcctttatgt ttattctttt
     3541 ttattgtata aacaatactc ctatactcat cctttgtcaa gcctaaattg aaaaaatttt
     3601 caatttgaaa caaaaaaaac ctagaaatac tacataaaat gtcattccta ggcgtattta
     3661 tgctattttt ctctggaaaa catgagtatt cagccagtca aaaggagctg aatgaactca
     3721 ttttccctcg cccaattcaa tgacacgatg acattgttgg gctacataag catcgtgggt
     3781 cacaataatg actgttttcc cttctcgatt catctctaag agaaacttca agaccaaatc
     3841 tctattttca ggatccagcg aacctgtagg ttcatcggct aaaatcagct ggctgggttt
     3901 taagatggct ctagcaactg caattcgttg ttgttcgccc ccagacaact cggagaccct
     3961 ttgatgcaaa gtagctgaca aacctactct ctctaaaatc tcttccacct ttttgagctt
     4021 gtctttctta ggcaatttca catatttcag cgccagcatg agattgtact cgaccgtttc
     4081 atcatcaatc agggcaaaat tttgaaacag ataagagata tgttcacgga ttattgtttg
     4141 cgacttagca gaattaaccg ctagatttgt ctgaccaaaa atctcatacc gtccgctata
     4201 atcaccatct atcaaaccca ataaatttaa caaggtcgac ttcccactac cactcttacc
     4261 aacaatagct accaaatccc cctgatcaat cctgagagat aagttatcca aaatcacttt
     4321 tcccccaatg gttttggtaa tatttttcaa ctcaatcata agatgccccc tttcaataac
     4381 tctactagac ttcttttctc catcctagaa gctaagccta gcacaaatag tatatccaga
     4441 catgtaaaac ctgcaaacag tagaagtggt aagaacgcat gggcaaagaa aatcaagact
     4501 agaagaggga aactatagcc cagcaagagc agaacgagga gaggacggta gcgatcgacc
     4561 agtttccacc ccataaactt cttggtaatg atatccctgc gcttcaataa gaaagttgtt
     4621 actagtaaga agtaggaaat catcatgcta aggagaccaa acaaagcaaa gagtaggtta
     4681 aaattccgaa cagcatctct ataagaatcc actttctctt gttgaatggc ttgaatagat
     4741 gaaaatttta aataatttcc atctgacaat ttctcaacta actctgtaat ctctttttga
     4801 tgttgaaccg tattttcaat tttaatcgga ttatttaagc cagttgttga cagggaggct
     4861 ttctcatccc acatcatatc agaatcattg accaagctaa taattggatt ggagagattt
     4921 tcctttcgct tatcactata tgggaaaaat gaccaatctc cttcataata ggcaatctcg
     4981 acatccatct cctctatcgt tcgtttttgc tgctcttcat acttcatcga atgaaaggca
     5041 attaacttcc ccaagagctg atttttatct tcttcacctt tcgtacttgc tggcatcaaa
     5101 ataacttttt taataccggt atctggtagc ttgaatccct tgctctttag aaaattgcga
     5161 ttggcatagt aaacatccac cgtatctgtt aactgatatt gctgaatctg ttctgattgg
     5221 acaaaatttt ttacaggaag actgctactc tgcacatagc ccgcctgcgt tttttctacc
     5281 aaatcctgat aaaatcgata gaaataatct gtagatttcc ctgaccctgc tagctcttct
     5341 tgccacagat tatcattgag tttgaaggtt tctaaggtca ggtaattacc ttgacttacc
     5401 cactgttgct gataagcaag ttctttgttt tcttgttcta aacttctgcc caccccaatc
     5461 agtaaggccg tcagtaaaat agttgtccct attttcatca cataattgaa gataagacca
     5521 aatttgaaag atgaaaaacc tttcagcaga gagctgattg tcattttttg gattaagagg
     5581 taagtcaacc aactgataaa gagataaagc tgcaacagca aaaaatgaga caaccacagc
     5641 ataggaaaca aatcttttgg cttataatca agcaagaaaa acacgcctag attgatcaca
     5701 agagccccac ctaggaggag gtaaaggttg ccttttacaa catcagctaa aacagcccta
     5761 tcttgaaaac caagtaattt ttgtacccca actcttttca tctccatcat cggttgatat
     5821 actgtcacta acacaagaag caaaatagcc aagacaaaaa caatggcaga taaaagcaaa
     5881 tctcgattta tgacttccac tgcacttttg taggtcggct ctagcacggt agcctggtct
     5941 atcttgaaaa aatcgctcca tttctgtaca atcctatcct tgtccatctc ttgtgtagaa
     6001 gttatcgtat agcgaccatt taaactacga gatgtatcct tgatataggt ttgaaaagtc
     6061 ataagctgaa taggtttggc ttttagaaag gtcggaatcg taccaagttt attggaaatt
     6121 tctttattac tatagactcc ttcaccatct gtggtaaaat caagagaaga aatcccaaac
     6181 tcttggtagg ggaaggtatc tttatcaaaa acaccagact tgaccacctc atcaccactg
     6241 tctgttttga tgatggagac tttatactcc tttgatacat cctcaaaaaa tcgaagaaca
     6301 gacgctgcag gttcgttaat atctttcaaa tacaaatcca aagaatctac agtctttccc
     6361 atttcttgaa tccgaaccac ttctcgtgta tagtttacat taaaaacaaa aaaagtggta
     6421 cacagaagca ggagtaacat acagaaatta ctgatttttt tcataaaagt gcctccacta
     6481 cagttttccc tctccaaata ggagagggaa gaattttact caaatccata gaaatattcc
     6541 attactgttg gcggaatctt ggtgagggaa gctctagccc atgcctcagg ttttgcataa
     6601 tccttagagg ttctaccccc atgtctaaca gttgctgtat gatatcgagt agaatgtaag
     6661 taatcagaat atccaaaagt tcctgtccaa cctactccat agttccattg accaccctca
     6721 acccatactg ctaaagcatt tgtagtagca agtataccac ataccattaa agatgcaaca
     6781 ccaattttaa ctaattttct catgatttat ttctcctatt atcgagagct gttccgtaaa
     6841 aacagctata gtaatttatc aacaagtatc gtagtccatt ggactcacct tccttctatc
     6901 tcatttcatt ttttaacaac gaacttactc ttaaccggga gaatggtgcg cttctgtatc
     6961 aggtccttga acaaccagag tagtattaag cacactctaa ctattactca agaatcagtc
     7021 caataaacaa tatggatagc gctttcaaat ttttaaactc ctatcccgtc cttttatcta
     7081 tataataagc ataagcgaaa atataataac tgtcaagtat ctgaagtgaa ttttataaaa
     7141 aaattacaag ccaaatttgt aaagtttaca ctaagccgct agggcaatcg tctatcagaa
     7201 tgtccgttta tttgtcaata atccgagaaa atcttgcaac gcttagaagt ctataaaaac
     7261 tatcaacatt tatatgactt gcgaatagca atcctgctaa acctttccac actctatcta
     7321 tacaatcaag ataaaaacat gtgtaagcaa atctgctaca ctttactgga ggacgccaag
     7381 aataagaaaa gctacgatag gcttgctatc tgctatgtct gtattgggat ttgtacagat
     7441 aatgctaaac ttatccaaaa aggtttctca cttctggagc tgaccgagga aacttctatg
     7501 ctgtctcatc tcaaaaaaga agtagagacc cattatcaac caaagaaatt ataaaaaaag
     7561 tcgagggagc tcctcgacct tttacatatt atttgttcgt tagttcttaa taccagccat
     7621 tgttaagcca gaagttttta gcagcagtcc atgaaccgta acgtcctgca acgtaggcat
     7681 ctgctacacg ttcttggttt tcagctgagt agtcaccgtt caggtatgaa tctgttaatt
     7741 ggtaacgtcc gatataacgt ccatttgtag ctgtatagct accacctgat tctttttgag
     7801 cgatccattc tttggcttct gcttcagatc cgcttacagt tgaaacaact gtttcactta
     7861 ctactggagc tgaaacagtt tcatcttgag cggctggtgc cgcataagta gctggcgctg
     7921 gtgttgcaac aggcgctaca gggccatcga taaccaactc ttgatcaaca taaatcaaat
     7981 gaatgttatc aatgtggttg ttttctgcca atttttcaac tgttgtgttg tgagtttcag
     8041 cgatttctga aagtgtatca ccttctttaa cagtgtaagt tgatgattct tgagcagata
     8101 caaatgatgg agcaaatact gcaaacaagg cagctactcc tgcaagagtt gctttaatct
     8161 ttttagttat tgatttcata ttcgaaaatt ctccttcttt ctatagttct atcataccct
     8221 ttaaatatta ccgtttcttg acacttttat gtagaaatat tacaaaatta ttttttattt
     8281 ccctaaataa ggtgtttttt gtcataaaag atactatttt tatgctattt gaggtattag
     8341 aaaggttttc atccacaatt aaagccaagc ccctcattct ttgatataat agagataaga
     8401 atttttataa ggggaaactg atgaaatcac ttcgttttca atctgtcttt gatatcatcg
     8461 gaccagttat gattggccca tctagtagcc ataccgctgg tgctgttcgt attgggaaga
     8521 ttgtctcttc catttttgat gatactccga cagaagttga attccaactt tttaactcat
     8581 ttgccaagac ctatcgtggt cacggaacag acctagccct tgttgcaggt attttaggaa
     8641 tggatacaga tgatcctgaa attccaaata gtctggaaat tgcccacaag cgtggtatca
     8701 agattgtctg gaccattcag aaagacagca atgcccctca ccctaacacc actaaaatta
     8761 ctgttaaaaa tgcccacaag accatcagcg tgactggtat ttctatcggt ggaggaaata
     8821 ttcaggtaac cgaactcaat ggttttgccg tctctctcaa tatgaataca ccgactatca
     8881 tcatcgttca tcaagatatt ccaggtatga ttgcccttgt tacagaggcg ctttcccgct
     8941 atggtatcaa tatcgcccag atgaatgtca ctcgtgaaaa agctggtgaa aaagccatta
     9001 tgattatcga agttgacagt cgcaactgtg atgaggctat cgaagaaatt cgaaaaatcc
     9061 ctcatctcca caatgtcaat ttctttaaat aggaggaagc atgttttatt ctatcaaaga
     9121 attggtcgag caagcagatc tagactttca aggaaatgtc gcagaactca tgattacaac
     9181 agagtttgaa ttgaccggtc gcgaacgtga agaagtcttc cttctcatgg aacgcaatct
     9241 ggaagttatg aaagcctctg tccaacttgg cctcaatgaa aataaatctc gtagtggcct
     9301 gacaggtgga gatgctgcca aattggatca ctacatcgaa aacggaaaaa ctctgtcaga
     9361 ttatacgatt ctctcggctg cccgaaatgc catcgcagtc aatgaacaca atgccaaaat
     9421 gggcttggtc tgtgccactc caaccgctgg aagtgctggc tgtctgccat ccgttctcac
     9481 tgctgctatt gaaaaattag acctcagcca cgagcaacaa ttggatttcc tctttgctgc
     9541 tggtgccttt ggactagtca tcgcaaacaa tgcctccatc tcaggtgctg agggtgggtg
     9601 tcaagctgaa gttggttcag cctctgctat gagtgctgcc gccttgactc tggctgcagg
     9661 tggaacacct tatcaggcca gtcaagctat tgcctttgtc attaaaaata tgctaggcct
     9721 catctgtgac cctgttgcag gtttggtcga agttccctgt gtcaaacgta atgccatggg
     9781 agctagcttt gctttcatcg cagcagacat ggccttggca ggtatcgaat ctaaaatccc
     9841 tgtggatgaa gtgatcgatg ccatgtacca agtaggagca agcatgccaa ctgcctttcg
     9901 tgaaacagct gaaggtggac tcgctaccac ccctactggt cgtcgcctcc aaaaagaaat
     9961 tttcggagaa taagcttatc aaataggaga aaccatgacc tctatcacag cgattttttt
    10021 cgatttggat ggaaccctcg ttgatagttc tatcgggatt cacaatgcct ttacctatac
    10081 ctttaaagag ctgggggtgc ctagccctga tgccaaaact attcgtggtt ttatgggacc
    10141 acctctcgaa agtagttttg cgacctgcct gtccaaagac caaatttctg aagccgtgca
    10201 gatatatcgt tcttactata aggcaaaagg catctatgaa gctcaactct ttcctcagat
    10261 tatagacttg cttgaggagt tatcgagcag ttatccactc tacatcacca cgacaaagga
    10321 tacatctact gctcaagaca tggcaaaaaa cttggaaatc catcatttct ttgatggcat
    10381 ttatggttct agccctgaag caccccataa ggcagatgtc attcaccaag ccttgcagac
    10441 acatcaacta gcaccagaac aagccatcat catcggagat accaagtttg atatgctggg
    10501 agctcgagaa acaggtattc agaaattggc catcacttgg ggatttggag agcaagcaga
    10561 tctactaaac tatcaacctg attatatcgc tcacaaaccc ttagaagttt tggcgtattt
    10621 tcaatagcat agactgggta acaattccat ttcagaagaa aatgaagtaa tactcaatga
    10681 aaatcaaaga gcaaactagg aaactagctg cgggctgctc aaaacactgt tttgaggttg
    10741 cagatagaac tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg
    10801 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata
    10861 tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtataa gtctctacat
    10921 aataaaacgc atattaccaa ggttgtgtaa cttgatacta tgcgtttttc ttttttattc
    10981 aatgtgggtt gtttgattag caaaggcgat aatagcttgc ttcaactcat ctccactgag
    11041 agtgcggaac tctccaatct tttgattgat ggattctaaa ggcatgacat taaccttacc
    11101 aacgaaaatc ttatccataa cttgattatc aacgagttcg gttgatacca agagttcttc
    11161 gtagagtttt tcacctgggc ggattccaac ttcaacgatt ggaatttcac tttcagtgtg
    11221 gccacttaga agcaccatct tcttggccaa gtcataaatc ttgactggtt tgcccatatc
    11281 aaggataaag acttccccat ctttggcata agcaccagca tggataacca gacggctagc
    11341 ttctggaatg gtcataaaat aacgggtcat gcggaagtct gtcaccgtta caggcccacc
    11401 ttcagcaatc tgacgttcaa agactggaat gacactacca cggctaccaa gaacattccc
    11461 aaaacgaact gcacagtagg ttgattggct acgttggtta aagccagtga caatcaactc
    11521 cgccacgcgc ttggttgctc ccataacatt tggtggattg actgccttat ctgtcgaaat
    11581 cataaccatc ttagacactt tagcttcatc aacagcctta gcaacattgt aagttccacg
    11641 gatattgttt ttgaaggctt cttttggatt gcgctccatc ataggaacat gcttgtgggc
    11701 tgccgcatga taaacaatag caggtttgta ctgctcaaag acttgcaaca aacgatcata
    11761 gtcttgaatg tccgcaatca caggtacata atcaatccct tggaacttac gaatcaattc
    11821 atgataaaca aggtagattg agttttcccc atgaccgagc aagacaatgc gttcaggatt
    11881 gaagcgacta acttgacgac agatttcaga accgattgaa cctccagctc ctgtgactaa
    11941 gatggtctta cctgtcagtt ctgcacccag acgcgattcg tcaagacgga tttcctgacg
    12001 acccaaaagg tccgtaatat caattttttg gaagccagta cctgcttggt gaaggccctg
    12061 aacaacagtt tcaaccttag gcatcttgta acatttgaca cccagcttat tacacatctg
    12121 caagatacgc tcatattctg acggatccag cgacggaatc gcaacgatga cacgctcgat
    12181 ttgatggcgt ttggctaatt caggcagatt gtcataagag cccaaaacag gaataccacc
    12241 aagtttttga ccctttttct tagaatcctt atccaaaata ccgaccagtt ctaattcact
    12301 ggttggatgt tggtaactat ccataaaaag agccccacca tcaccggcac caatcaagaa
    12361 ggtccgacgg tgttctccat caccactacc ttttttgcgt ctggagtaga ttaactgcca
    12421 agtaatccgt ggcaataaaa tcaagaaggt actcaacaag ataaagagaa tgatgaaacg
    12481 gatggagaag agtggcaaga aggcataaca gatactatat gacaagacac tgctagcagt
    12541 cacaccaaaa aagattttca tgaaatccgt aatcttgctg taacgactaa tgctcgcgtt
    12601 caacccccaa aaaccaatca tcaattgata gaacaggaag gccaaactcg tatagataat
    12661 gtagtcaaca ggtgctggat taatcagccc ataaaataaa atataagata caatgatgga
    12721 aaccaccata ctgaaaatat caaatattcc ccagaaaacc tgcttttgtt gtttatttaa
    12781 aatttccacc agatcaatca cataatctgt tagtttttta ttcatagaaa tctactcctc
    12841 cttaaaagag ttttactata ttgttattat agcatatttt tgtttatttg gcttatttta
    12901 tccaaataaa ttattgttct cttgtactcc ttcttccatt taaatcctct aattaaacct
    12961 acaatcgtcc caaagccata agcaaagtga atggaaaata aaagaaaggg catcacaatt
    13021 agaaatccat ttttatgttt taataaagtc agcaaagtga gtagtgacaa aagtagaaaa
    13081 taggcaccta atagtaaagt tatgaatacg aatgtgatcg gtaacaatgc tagactaaac
    13141 acaagactca aaacaaataa acaaggaaca tagtgaaata atgataaaca cttaaactga
    13201 acatgacttg tcaagccaat ccacaaacca tttaaatact tttgatgcag cattttcttg
    13261 aatgttggtc gaatatactg ataagataga atacttgggc tatagcggat tttataacca
    13321 tattctcgaa ttctataatg aatatcatta tcttcagttc ggccaagttg ctcatttact
    13381 aaaccaacct tctggaaaac ctctcgttta tacattccat gaaaaataga agaaacatat
    13441 ctatcctcag aactatttcg ataattggca atgctactgc caaacatatt ttcctcaaca
    13501 agatgcaagg tctctgccca ttttcctttt ccttcgacaa tcgtcggtct aggcccccca
    13561 cagacaaatt caccttgttg aataatagcc acattgttca ttacaaaact ctcagtaact
    13621 tttgaatgag catcaatttt taaaataagg tcccctacag aatgtttaac tcccaggtta
    13681 aaaccactag cttgattttt cttaggattg ttatacaatc taattgagtt aaactctgta
    13741 tcttccttta taaattgctg aatgatagct gtggtcccat ctgtggacat agcatttata
    13801 aatagaattt caatattctc tttaggataa gtttgatttt ttaagtcttc aattagacca
    13861 ggcagatatt tttcttcatt gtatgcactg attacaatgg atacgagaag actttcaaca
    13921 ttttgagtca ctactactct cctgaatttt tccttccacc attctatcat aattttcaag
    13981 actttcccat ttttatcttg aaagccagaa accttacttg acattatagt gaaaaagcct
    14041 taaaataagc tgttattaaa atcagctaga agaggtatta tatgaaaaag caatcactct
    14101 tttttgttcc aggtattatc ctgattggtg tttccttgcg aactcctttt actgttttac
    14161 ccattatttt gggaaatatt tcgcaaggtc tggaggtaga agttagttcg cttggtgtct
    14221 tgaccagcct gcctctcctt atgtttaccc tcttttcacc attttctacc cgactggctc
    14281 agaaaatcgg cttggagcat ctcttcacct acagcctctt cttcttgacc atcggctcac
    14341 ttattcgact aatcaatctg cccctgctct atctaggaac cttgatggtt ggggcaagtg
    14401 tcgcagtcat caatgtcctg cttcctagtc ttatccaagc caatcaacca aagaaaattg
    14461 gttttctgac caccttatat gtaacgtcta tggggattgc aacggctctg gcttcctatc
    14521 tagctgtgcc cattacacaa gccagttctt ggaaaggact tatccttctc ctcacgttac
    14581 tctgtctagc aacttttttg gtctggctcc caaatcaccg ctataatcat agactagctc
    14641 cacaaaccaa acaaaaaagt caaataaagg tcatgcgtaa taaacaggtt tgggcaatta
    14701 ttatcttttc aggttttcaa tccttgatct tttacaccgt catgacctgg ttacctacca
    14761 tgtctatcca tgcaggtcta tccagtcacg aagctggctt gctgacttct atcttatctc
    14821 tgattagcat tcctttttca atgaccatcc caagcctgac aaccagttta tctactcgca
    14881 accgtcagct catgctcact ctggtttcac tagctggtgt ggtcggcatt tccatgctct
    14941 ttttcccaat caataatttc atttactggc ttgccatcca tctcctcatc ggaaccgcaa
    15001 ccagcgccct cttcccttat ctcatggtca acttttcact caagacaagc gcccctgaaa
    15061 agacagccca attgtccggc ctatctcaaa caggaggcta tatcctagca gcctttgggc
    15121 caaccctctt tggttacagt tttgacctgt tccactcttg ggtaccatct gtagctgccc
    15181 tcttgctcat cgatatcctg atgactgtgg ccctctttac agtggacaga gcggataaga
    15241 tcctttaaac ttctagtttt agctagaagt tttttatata taaaaatttt acacatttct
    15301 cttgacaggg ctttctttta gatgtacaat gtatgtgtag aaaaattata tataaaaatc
    15361 ttacacatta gaaaaggagg tttcccatgt actttccaac atcctctgcc ttgattgaat
    15421 ttctcatctt ggccgtactg gagcagggtg attcttatgg ttatgagatt agccaaacca
    15481 ttaagctgat cgctaatatc aaagaatcca cactctatcc cattctcaaa aaattggaag
    15541 gcaatagctt tctgacaacc tattctagag agttccaagg tcgcatgcgc aaatactact
    15601 ccttgacaaa cggtggtata gagcagctct tgaccctaaa agatgaatgg gcactctata
    15661 cagacaccat caatggcatc atagaaggga gtatccgcca tgacaagaac tgaatacctg
    15721 actcagctag aactctatct caagaaacta cctgaagctg accgtatcga agccatggac
    15781 tatttcagag agctctttga cgatgctgga gtcgaaggag aagaagaact catcgctagt
    15841 ttgggaactc ccaaagaagc ggcccacgaa gttctatcca atcttctcga taaaaaaatc
    15901 aatgaagcac ccgctcaaaa aaataaccga caaattttac atatcgcctt gttagccctc
    15961 cttgcagcac ctatcggcat tcctctggga atcgccatcc tcgtgaccct gttcgcaatc
    16021 cttgtagccg ctttgactgt cattctggct ttctttgcag tttccatact gggtatcatc
    16081 ggcggattcc tatttttagt tgaaagtttc actatcctcg cccaagccaa atcagccttt
    16141 atcttgattt ttggttctgg tttactggct atcggtgctt cttcgctagt tttacttggc
    16201 atttcctatg tagctcgctt cttcggtcta ctcattgttc gtctggtaca atttgttctt
    16261 aaaaaaggaa agagaggtaa tcagcatgcg taaatggaca aaaggatttc tcatctttgg
    16321 tgtggtgact accgttatcg gctttatcct gctttttgta ggtatccaat ctgacgggat
    16381 taagagccta ctttccatgt ccaaagaacc tgtctatgat agccgtacgg aaaagctaac
    16441 ctttggcaag gaagtcgaaa acctagaaat tactctccac caacacacgc tcaccatcac
    16501 agactctttc gatgatcaaa tccacatttc ttaccatcca tctctttctg ctcaccatga
    16561 tcttatcacc aatcagaacg atagaactct gagtctcact gataagaaac tgtctgaaac
    16621 tccgtttctc tcttctggaa ttggtgggat tcttcatatc gcaagtagct actctagtcg
    16681 ttttgaagaa gttattctcc gactaccaaa agggagaact ctaaaaggga tcaacatctc
    16741 agccaatcgc ggacaaacca ccatcataaa tgctagcctt gaaaatgcga ccctcaatac
    16801 aaacagctat atcctccgaa ttgaaggaag tcgtatcaaa aacagtaaac tcacaacgcc
    16861 caatatcgtt aatatctttg atacagttct tacagatagt cagctagagt caacagataa
    16921 tcacttccac gctgaaaata tccaagtcca tggtaaggtt gaactgactg ccaaagatta
    16981 tctcagaatc atcctagacc agaaagaaaa ccaacgaatt aactgggaca tctcaagcaa
    17041 ctatggttct atcttccaat tcacaagaga aaagcctgaa tcaagaggta cggaattaag
    17101 caacccttac aaaactgaaa aaaccgatgt caaggatcaa ctcattgcga gatctgatga
    17161 taatattgat ctaatatcca caccaagcag acgttgacaa aaacgcttac atctgctact
    17221 atggaagcat atttatctaa ccaaaaggag gttctaccat gaaacaagaa tggtttgaaa
    17281 gtaatgattt tgtaaaaaca acaagcaaga acaagcctga agagcaagct caagaggttg
    17341 cagacaaggc tgaagaaacg atagccgatc tcgatacacc aattggaaaa aatactcaag
    17401 tagaggagga agtctctcaa gctgaagtcg aactggaaag ccagcaagaa gagaaaattg
    17461 aaactcctga agacagtgaa gcgagaacag aaatagaaga aaagaaggca tctaattcta
    17521 ctgaagaaga gccagacctt tctaaagaaa cagaaaaagt cactatagct gaagagagcc
    17581 aagaagctct tcctcagcaa aaagcaacca cgaaagagcc acttcttatc agtaaatctt
    17641 tagaaagtcc ttatatcccc gaccaagctc caaaatctag ggataaatgg aaagagcaag
    17701 tgcttgattt ttggtcttgg ctagtggaag cgatcaaatc tcctacaagt aagttggaaa
    17761 caagtatcac acacagttac acagcctttc tcttgctcat tctgttttct gcatcttcct
    17821 ttttctttag tatctatcac atcaaacatg cttactatgg acatatagca agcattaaca
    17881 gtcgcttccc tgagcagcta gctcctttaa ctcttttttc tatcatctct atcctagtag
    17941 cgacaacact cttcttcttt tcattcctct tgggtagttt cgttgtgaga cgatttatcc
    18001 accaggaaaa ggactggacg ctagacaagg ttctccaaca atatagtcaa ctcttggcaa
    18061 ttccaatctt cctcactgct attgctagtt tctttgcttt ctttgatagc ctacgattta
    18121 cagccctctt gtgtgtgatt agcattggaa tcattctgct tgccagtctc catatcatta
    18181 caagaccaag tcaagcaagt gaaaccgact ccttctatca attattcttg tctgtccttg
    18241 tgaacggagt tattatcctc ctcttctttg tagctgaagt ggcactgatt ggagattatc
    18301 ttcgtatctt ggcctttctt taaaattcaa tcctgtcatc cttggcagga tttttctatt
    18361 tcaaaaaagc aagtcgattg acctgcttct gctttacttt tcttgtgcta atgttttaaa
    18421 atcttgtcct aggatgggtt gtacttctaa ttgattggcg tctagttggt ggtaaaatgc
    18481 tgatggtagt gactctagga attttgcata gtccaagcgg gcgatggctt catagtcttc
    18541 tggtttagag ccatctcctg tgatttgaac caagtcgatc tcccactgca cttccttatc
    18601 caccaattgc tccatatagg cagcaggaac aaatggttct ctcggtaaaa cggtcttgac
    18661 gccttgagcc aaatgataaa caccgtgcac ttgcccttct ccatcctgat agaaggaaac
    18721 tcttgcaaag taggcatctg tctcttgaac cgcacgcaca cgcgcttcaa actgggccaa
    18781 gccatctgcc tctaaaaagc ttttcaagtt ctcacgacta aagaaaatag gattaaaaat
    18841 tccttggcaa atcgtaaaac gagctacagg gtctgctaga taggcttgaa tacttgcttg
    18901 gaaataagct tcaaaatcaa aaccatctag cccttcaata tcagtccctg tataagcaag
    18961 caaagcatct aaaaatgact tgataatctg ccagtctgtc tttgttagta ggtcaggaag
    19021 atcgacacgg taagcctttt gaccatcagc ataactgacc ttaaaaagaa attgtgattg
    19081 ccccattatc gcacagacga ttaagaggct gacggagata gactacatca tagctatgtg
    19141 actccaaacc gtctagcaag gccaagatcg acttggcatt caaaatctct tgttctccta
    19201 aaatgctctg tttatttgga ataaaaaatg tttttgccat aactggactc ctttgaaatc
    19261 gtttacatac agtatacctt atttttctga aagatacaaa aacaaacttt atattcttcg
    19321 aaaatctctt caaaccgcgt cagcttcgcc ttgccgtaga tatatgtaac tgacttcgtc
    19381 agtcttatct acaacctcaa agcagtgctt tgagcagcct gcagctagct tcctagtttg
    19441 ctctttgatt ttcattgagt attagatttt tgagtacaaa gcatagaaaa acagcccctg
    19501 aggactgttt gatcttatac agtagctgcc tgatccaagc tttcaccgat agcggctagg
    19561 cgctcaatca cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga
    19621 acacggcact cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag
    19681 atacgacggt tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca
    19741 aaccagtctt tccctacgat ggttgggttg acatggttga catcaacggc aatacgctcg
    19801 tcaaagacgt acattttccc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa
    19861 gttgcgattc ctccgtgcaa ttggccaaca tctttgtaac cttcacgaac catccagcct
    19921 gagaatttct cacagcgaac accacctgta cagtaaacca cgacacgctt gtccatgaat
    19981 ttttctttgt tgtcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga
    20041 atagccccac ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg
    20101 gtatctttat caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt
    20161 tcaagtgggt tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct
    20221 ttcttgtagc gaacaaacat cttcttgaag gcttgttcac tttcttcgtc aatcttgaac
    20281 cagagatctt ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca
    20341 tagtcacctg aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaagg
    20401 ccgattgatt tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga
    20461 gtataaaggt agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct
    20521 attcttaaat tttctaaatt ttttatttat ctacactagt atacctgctt gagaaaacga
    20581 atgcaattta tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat
    20641 aaggacaaga gaaaacaggg atatctatat ccttgtgccc aaaaaaccac tgccctcccc
    20701 agaccaatct gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat
    20761 caatttagct gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt
    20821 caatactttt tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt
    20881 atctgggtct acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat
    20941 agctgagatt tcagatttca cattgtcagt attaaagata aatccaagcg ctggagattc
    21001 tttagcttct gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt
    21061 gatgtaaagg atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta
    21121 gccatcaaga acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc
    21181 tggaccgtaa acaagaccgt tcaagagttc tgggttcgta ttcaagaggt tcaagatttc
    21241 cattgatttt tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt
    21301 ttggtttttc ttgatgaagt tagtaattgg tttgatttgg atatctttgt tggcaacacg
    21361 tgaaagcaag ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt
    21421 atcttgttga aggtcaaagg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc
    21481 ttcatagaat ttgtgaagag tcttcaagtg ttctttgaaa cgaggtactt cgtaacggtt
    21541 tacaacttta gtagtatcgc cttcaaggtc aataacgaat ggaagaccgt ttgctactgg
    21601 gtagtcaaag ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg
    21661 agctttttct ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga
    21721 aatatcgata ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc
    21781 tacgttggct gcaactggta cagcgtaaat cttaccattt acagtattac ccttgatata
    21841 agctggatca agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc
    21901 agcataagca cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt
    21961 ttcaccagat gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg
    22021 gatatccaat ttagcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc
    22081 atccaagtta tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga
    22141 atcagcagct tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccag
    22201 agcgactaca ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc
    22261 tttttttatt tttaaactta taaacaatgt aatgatctta tactcaatga aaatcaaaga
    22321 gcaaactaga aaactagt
//
LOCUS       DAVLTM010000021        22116 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLTM010000021 DAVLTM010000000
VERSION     DAVLTM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.021"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..221)
                     /locus_tag="VYN16_000713"
     CDS             complement(<1..221)
                     /locus_tag="VYN16_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1403278.1"
                     /translation="MRKYDIPKLLLSGENQGVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVSENTKNKDFYISGVNDPDKVLDE"
     gene            complement(382..1095)
                     /locus_tag="VYN16_000714"
     CDS             complement(382..1095)
                     /locus_tag="VYN16_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1403279.1"
                     /translation="MAKNSPQDLKNREYFSNNLNRVMKEKGIRQIDLHNALEIPKSTI
                     TGYVKGRSMPTSGNLQKLADYLGVKKSDLDPRFSDKDVYKEFLYMLDKQLDEEKYLVK
                     NVSKDKIFQKVKEREKRDKEIIELAKSKNFPISPEEFLTTITESLNEYNPKDNYEVLN
                     ALALKTSNILFWLDIVFQKTSSEFYIVFEDKEFNQEYYNKLYKKFENLFEEIKSLALQ
                     EAKKDKENLDDFALLWGID"
     gene            1248..1477
                     /locus_tag="VYN16_000715"
                     /pseudo
     CDS             1248..1477
                     /locus_tag="VYN16_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1493..2119
                     /locus_tag="VYN16_000716"
     CDS             1493..2119
                     /locus_tag="VYN16_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV1403280.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIETWEAKPKHAHSTMNNLLLKAVTDMNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            2491..2943
                     /locus_tag="VYN16_000717"
     CDS             2491..2943
                     /locus_tag="VYN16_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403281.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            2940..3140
                     /locus_tag="VYN16_000718"
     CDS             2940..3140
                     /locus_tag="VYN16_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403282.1"
                     /translation="MTLNLDNMTQAEFDEVMAEIKEKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKNYQARA"
     gene            3142..3357
                     /locus_tag="VYN16_000719"
     CDS             3142..3357
                     /locus_tag="VYN16_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403283.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSAQFERENQQRI
                     ETPSEDLSPDYGRYIQLAGVRVYGGTE"
     gene            3354..3695
                     /locus_tag="VYN16_000720"
     CDS             3354..3695
                     /locus_tag="VYN16_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1403284.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRHSVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTVLLNADLESY
                     KETLKEVGGYV"
     gene            3688..3969
                     /locus_tag="VYN16_000721"
     CDS             3688..3969
                     /locus_tag="VYN16_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1403285.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEARDTVKPRLTGLISAQEVM
                     DELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYKISDIWKFLGVENGKV"
     gene            4104..4970
                     /locus_tag="VYN16_000722"
     CDS             4104..4970
                     /locus_tag="VYN16_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV1403286.1"
                     /translation="MTIYEAKGFQSNLVYPFDKIEPFQYIERFKPLVVPEGADIEEYK
                     RTQAPYCISGKVTPEKHGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKAISSALFGY
                     SYILYPTIKHSLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPASYQKIVEHGLDYPVPQTTAEPVKQNAASLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            5023..6492
                     /locus_tag="VYN16_000723"
     CDS             5023..6492
                     /locus_tag="VYN16_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV1403287.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNENTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVSPEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            6855..7022
                     /locus_tag="VYN16_000724"
     CDS             6855..7022
                     /locus_tag="VYN16_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403288.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEIDDFMATVEVLDVKFSGTPSSESF
                     NSVATVLVLYR"
     gene            7106..7636
                     /locus_tag="VYN16_000725"
     CDS             7106..7636
                     /locus_tag="VYN16_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403289.1"
                     /translation="MTLKTFSSKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPV
                     ISLTYKDKGTLVAEYVEDHKLNKTFKRICDSFKDYHKQPGEAEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFATLEMLGDESISLLQKLD
                     VEGEYKGLAGYTEHLK"
     gene            7707..8216
                     /locus_tag="VYN16_000726"
     CDS             7707..8216
                     /locus_tag="VYN16_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403290.1"
                     /translation="MKQLAIEAITKPMKLRGISKGIAELDGQRLEIDLDSLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKHTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKQKRYYKHYQKFINYTRKGDSF
                     WLNGLSSLR"
     gene            9423..9842
                     /locus_tag="VYN16_000727"
     CDS             9423..9842
                     /locus_tag="VYN16_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1403291.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELETLEGGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMKISELIDKLANPLERSVLRLFYL
                     NDLVVSEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
     gene            10331..13162
                     /gene="uvrA"
                     /locus_tag="VYN16_000728"
     CDS             10331..13162
                     /gene="uvrA"
                     /locus_tag="VYN16_000728"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1403292.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            13155..14216
                     /locus_tag="VYN16_000729"
     CDS             13155..14216
                     /locus_tag="VYN16_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1403293.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            14348..14746
                     /gene="spx"
                     /locus_tag="VYN16_000730"
     CDS             14348..14746
                     /gene="spx"
                     /locus_tag="VYN16_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1403294.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            14812..15381
                     /locus_tag="VYN16_000731"
     CDS             14812..15381
                     /locus_tag="VYN16_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1403295.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            15468..15734
                     /locus_tag="VYN16_000732"
     CDS             15468..15734
                     /locus_tag="VYN16_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1403296.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            15738..16157
                     /gene="ruvX"
                     /locus_tag="VYN16_000733"
     CDS             15738..16157
                     /gene="ruvX"
                     /locus_tag="VYN16_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1403297.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            16173..16478
                     /locus_tag="VYN16_000734"
     CDS             16173..16478
                     /locus_tag="VYN16_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1403298.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            16891..18141
                     /locus_tag="VYN16_000735"
     CDS             16891..18141
                     /locus_tag="VYN16_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1403299.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KTLEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            18138..18680
                     /locus_tag="VYN16_000736"
     CDS             18138..18680
                     /locus_tag="VYN16_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1403300.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            18826..20358
                     /gene="cls"
                     /locus_tag="VYN16_000737"
     CDS             18826..20358
                     /gene="cls"
                     /locus_tag="VYN16_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1403301.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            20616..>22116
                     /locus_tag="VYN16_000738"
     CDS             20616..>22116
                     /locus_tag="VYN16_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1403302.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVK"
BASE COUNT         7072 a         4052 c         4887 g         6105 t
ORIGIN      
        1 aattcgtcca gtactttatc gggatcatta actccagaaa tataaaaatc tttattcttt
       61 gtattctctg agacacctag aattaacaga ccattatttg tattagcgaa ggaacaaatt
      121 gttttcatac cgtctttagg gaaagaattt ttagcttctt tgaattcaac accttggttc
      181 tcccctgata ataacaattt tggtatatcg tattttctca ttttttctcc aatcaatttg
      241 cacacgcatt cgcacacgcg cgggaaaaat agtttatttt tagttagcta acagtgctat
      301 taaaggtttt tttgaaagta gattattcgc acacgcattc gcacacgcgc ttcaggattg
      361 ttgaacattg tggcgcgtgg gttagtctat accccaaagt aacgcaaaat catctagatt
      421 ttctttatct ttcttagctt cttgaagagc taaagactta atttcttcaa atagattttc
      481 aaacttttta tataatttgt tgtaatattc ttggttgaat tccttatctt caaaaactat
      541 gtagaattca ctacttgttt tttgaaatac aatatctagc cagaatagga tattacttgt
      601 ctttagcgca agggcattta aaacttcata gttgtctttg ggattgtatt catttaatga
      661 ttcagtgata gtagtgagaa actcttcagg actgattggg aaatttttac tctttgctag
      721 ttcaattatt tccttgtctc ttttctctct ctcttttact ttctgaaaaa ttttatcttt
      781 tgaaacattt tttacgaggt atttttcttc gtctaattgt ttatctaaca tatataaaaa
      841 ttctttgtaa acatctttgt cagaaaagcg agggtcaaga tcagattttt tcactcccaa
      901 ataatcagca agtttttgaa gattgccaga tgttggcatt gatctacctt ttacataacc
      961 agttattgta cttttaggaa tttctaaagc attatgcagg tctatctgtc tgataccttt
     1021 ttctttcata acacggttaa gattattaga gaaatattct ctattcttta aatcctgagg
     1081 gctatttttt gccattgtta aaaactccct tactttaatt ctacaacctg attataacac
     1141 aaaaaagaca aagtacgaaa aaaataaaat ttttcaacaa cagcgttgac aaagtacgaa
     1201 aaaaatagta ccatgatttt atcaatcttg aaaggagcta taagaatatg gcacaatgga
     1261 cgctaagagc gtgtcgagta aatgcaggtt ttactttgcg ccaagtggct aagaaggtca
     1321 acaagaattt tcagactatc tcaaagtacg aaaaagatag tacgctaatc ccttttgagc
     1381 tacttaaaga gttatcggaa ctgtatcagg taaaactgga cgatattttt taggagatag
     1441 tacgaaaaaa atagaactaa atcaaaagga aatctaagaa aggagcaaac caatggaatt
     1501 agtctacatg gacggtaaga aagagccgta tacactgagc agtattgttg cggagtgtgc
     1561 agaagttaag cacagacatt tgaagatttt gctgaataaa catcgagagg acttcgagag
     1621 tttcggaaag gtgcaattta aaatttcacc ttcagaaagt gggcaaaatg tacgggacta
     1681 tattttgaat gagcaacaag caaccttgct gatcacttac ttacgaaata cagaacccgt
     1741 aaaagagttt aagaagaacc tggtcaaagc attctttgaa atgcgtgagg agctttctaa
     1801 gttccgtatg cagagggcgc tagaaaagcc aaaaagaaaa accttgcatg acagtataga
     1861 gacatgggag gcaaaaccca agcacgcgca tagcaccatg aacaacctgc tactaaaagc
     1921 agtaactgac atgaacgcta agcagttaag ggaagaacgt gggggctata acggtattga
     1981 tagcttgacc agtgacgagc tggagcaata ccaggcattt gaggatatgg taatagccat
     2041 gatcggcttg aatatgagtt accaagaaat caaggctatg gcattcagaa ataaaaaaac
     2101 acgccaaaga agcgcgtgag agcaacaaaa aaggcttagc aataaccaca ccgcagagcc
     2161 tttcacacta acactaaaac gaatttaaca aagcaggcaa gctattatca aagaggtttt
     2221 agtaaagatt tatacctaga ttatagcata tttagggcaa tttgaccata cggagagcgc
     2281 caactcttta aactggtact ttctcatgct ctcaatttgg cgactcagag cgtgaggcta
     2341 tgaattgcaa gaaaaactgt ataagaaaca accattaaaa acccccacac tctcggtcgg
     2401 caaacttctg agcgtgagga tatccagtat agtaaaaggc attaaaagct cttagagcaa
     2461 ttacacaaca tagaaacaaa ggtaaaaaac atgacagaaa caacatacga gaatttaact
     2521 agacatatag acagaattag cgcagaaatg cgagaaattg gcgaaaaaaa tgggcttagt
     2581 agactttcat tattggcaaa tcagacaaaa ttcatcaaag aaaatttaca ccgtttactt
     2641 tggattgaac ttcctgaatt gaatgacagc cataaaatcg aggcagtttc taaaagcact
     2701 acgggaatgt ttttccacgc tggtattttt gaaatggacg ctatgcgaca agcattcttt
     2761 aaacgccaag ctatgcactt ttttgacaac gaagcagagc aacaggcgta tatagaacat
     2821 gctgaaaagg agtatttaga ggctactata accttaaaag atattctttt taactctaaa
     2881 aatggaactc agagagtaaa taaaagttgt cttatagaga agtttgagga ggcaatgcaa
     2941 tgacactaaa cctagacaac atgacacaag cagagtttga tgaagtaatg gctgaaatta
     3001 aggaaaaacg ccccaacctc ttccagttta ttgctgattt tgtagatcga aaagtaagca
     3061 ctgaggaggt agacgaatac ctgaagatga aacatgaaaa acaagtggaa tttatcaaga
     3121 attatcaagc gagggcataa catgaatgag ttagatttga ccaatacaca atcggtaatc
     3181 tttatggtgg tattgattgg cttactgctt tacctaaacc accgagaccg caaaaaaagc
     3241 gcccaatttg agcgagaaaa ccaacagagg atagaaacac ctagcgagga tttaagccct
     3301 gattatgggc gttatatcca gcttgcaggg gtaagagttt acggaggaac tgaatgagtc
     3361 tatcagagaa tgacaaacga gtattaagac taatcaaggt aggggctgag aactccataa
     3421 caggggcaga aatcagcctt ataactaagc tggcggaaag aaccgtacaa gatattatca
     3481 gccgtctaat cacgcgtcat agtgttccta ttgttggggt tcgccatggg acatttagag
     3541 gatattttat cccagctaac aaagaggagc tactggacgg agcaaaggca ttctataacc
     3601 aagtacagga agaacagaaa cgcctaactg tccttttgaa tgctgattta gagagttaca
     3661 aagaaacctt gaaggaggtg ggcggatatg tttagcctaa gccgagaaag tgagcaagac
     3721 ctagcgcatg gcattctgga ggtagtagaa agatacctgg aagcgcgtga tacagtcaag
     3781 ccgcgactga caggtttaat atcagcccag gaagtcatgg acgagttaga tataaaatac
     3841 atgacactcc aaaagtggga aaaagcaggg ctaagacggt atcagccacc gctagaagat
     3901 actaggaaaa tctattataa aatatctgac atctggaagt ttttgggggt ggaaaatggc
     3961 aaggtatagt attcatccgg cagataataa agggcattat cacaatatta aattttacaa
     4021 agagcgcagg ccaaccctag accagctaaa ggaacagcaa aggcttaaaa agctgaagaa
     4081 aaaacgcaaa aaggggcgat agtatgacaa tttatgaggc taaaggcttt cagagtaatc
     4141 ttgtctatcc atttgataaa attgagcctt tccagtatat tgaacgattc aagcccttag
     4201 tagttcctga aggggcagat attgaggaat acaagcgaac tcaagcgcct tactgtataa
     4261 gtggcaaagt gacgccagaa aagcatggca gttacaagcg aaacaactcc agtctaatct
     4321 atcgggattt gattttcctt gattatgacg atatacaggg gacgtctgag ggctttataa
     4381 aagccatttc tagcgcttta tttggctact cttacatctt atacccgact atcaaacata
     4441 gcttagaaaa gccccgtttt cgccttgtag tgaagcctga caacgtgatg aatgaggcga
     4501 cctataagca ggtagtgaaa gagattgctg acaagattgg actccccttt gatatggcca
     4561 gtttaacatg gtcacaactc caagggctac cagtcacaac aggcgaccca gcaagttatc
     4621 aaaagattgt agagcatgga cttgattatc cagtaccaca gacaacggct gaaccagtca
     4681 aacagaatgc ggcaagcctt ccatacactc caaggccaag cgggcagaaa tctatgacca
     4741 tgaggatcat tgacacgctt ttcaacggat ttggagacga gggagggcgc aacgtagcac
     4801 tgacacgatt tgtaggctta ctctttaata agtgggttga ttgtgaccta gagacagcct
     4861 atgaacttac aaagatagcc aatagcgtga cagttgaacc attgccgatt gaggagctag
     4921 accgcacttt tagcagtata gcacgggctg aatatagaaa gagaggttag aaccatagaa
     4981 ctagaagaat tagcaaacct aaaaagtgaa atcttggagg taatggagca agaacaccct
     5041 cctagaacga tgaaggagct tgaaaatcgt atctttaaag ctggagaaga atggcgggca
     5101 gaacatactg aaactaaggt aaatgagaac acaggggacg ttaccgaaaa ggttgccatg
     5161 ccccaaacct tcacagtagc taaaatactg agcgagattg tcacatttac ttttatcagc
     5221 aagagcaata tacctgatta tagcctactc tatatctatg atttagacga gggtatctat
     5281 accgctagta atgatctatt caatcttttg tgtaagacct ttgacgttag aattaaaccc
     5341 agagagtggc cacagattaa gttaatggtt agaacattgg caaagatacg aaaaccttta
     5401 gaaagtgcta acctcattcc cgtacagaac ggcattatca acttagaaac taaggaacta
     5461 ctccccttta gtcctaagta tgtaattaca agtaagataa gcacggccta ccacgcgcct
     5521 aaacgagtcc caacagatag agagggaaaa acttttgatg attggttgaa ctcaatcgct
     5581 tgtaatgata gtgaactggt aacactgttt tggcagatta tccttgaagc tattaaccct
     5641 aaccatactc gaaataagtt tgctatcttc tacggggacg gtaacaatgg taaagggaca
     5701 tttcagcgct tccttatcaa cctgataggg gaaagcaata tatcagcctt aaagcctgca
     5761 cagtttgctg aaaagcataa cttggagacc ttggtaggta aggtttgtaa tattggggat
     5821 gaagcaccta acgaatactt aaaaaatccg tctgatttaa tgagtatcac tagtggcgac
     5881 actgttctag tcaatccaaa aggacgccca gcctttgaag ctactttcaa gttattcaat
     5941 atcttttcag ggaattatat ccccaatggt ggcaacaaga caaaaggctg gtatcgtaga
     6001 attatgattg tcccttttaa tgctgacttt aacggggaga aggaaaaacc ttggataaaa
     6061 aatgagtttt tagcaaacaa ggaagtccta gaatatgccc tctacaaagc tatcaatcaa
     6121 gagccattta ctcactttat cgaacctaaa gcagttaaag gactcctaga agaataccag
     6181 gaagataatg actacttact ttcatgggtt aagcatgaat acatggaaaa aggttggcat
     6241 gagctggacg tagtacctgt ttttatcgtt acaagatcac taaagcacta tgccgaagat
     6301 atgggaatac ccaagcccaa tgtttacggg gctggtaaag aaactatcaa gcacttacaa
     6361 cagttgacac ctaatcggta ttctctaaga aagaaaagag tttctccaga agattttgaa
     6421 aaactagagg catgggacgt tgacaagata aaaatgcgga cacctcaaca ctctatcact
     6481 aaacaagaat aagtgtgacg ttcttttttt gcttaaaccc ttgataatac tgattttctt
     6541 gaaaaaagtg tgacgttgtg tgacgttctc aaaaaagaag gtcacacccc ttgaagcctt
     6601 gatatatcta actttagggg aataggtgtg acgttgtgac attctttcca actcttatat
     6661 atagagaaaa atacagtatt tatatatata ggaacgataa aacaaaagaa cgtcacaggg
     6721 tcacaggggt ggtgaaacac ctagagcctc aagggtttga acgtgtgacg ttctttgtca
     6781 caaaaggtca cagaaggtca cagaaaacga aaaatattac ataatttacc ttaaaacaaa
     6841 gaaatgacaa caacatgaaa gttaaactat ttgaatatac tagacgatgg gaaaaagaag
     6901 actttgagaa agagatcgat gacttcatgg caactgttga agtgctggac gttaaatttt
     6961 ctggtacacc atctagtgaa tcctttaatt cggtggctac cgtattggta ttatacagat
     7021 aacaaaatgg ggtagatagt accgaccccc ttgcaatggt gtaactacta gtgacaccct
     7081 taaaaacaga aagaggaaaa acaacatgac attaaaaaca ttttcatcta aagccaaaac
     7141 atttactttc acttacgaat ttaaagacct agatacagct ctggtagcag gtcacgcgtt
     7201 actgggatat atgactggaa catattgcca gcctgtaatc tcattgacct acaaggataa
     7261 aggcacgcta gtagctgagt atgtggaaga tcacaagcta aacaagacct ttaagcgtat
     7321 ttgtgacagt tttaaagact accacaaaca accaggagag gctgaagcat ttgaagaacg
     7381 ttacaaacgg gagcgcgtgc tccaactcaa agagtcagag gactttgaga gtttactgaa
     7441 taaggtcaca gactacgagc tggaactatt agactatgct gaccgcctgc tttctgataa
     7501 gcctatccct atggactcta tgactgcttt tgctacgttg gaaatgctgg gcgatgaaag
     7561 tattagccta ctccaaaagt tggacgtaga gggcgaatat aaaggccttg ctggttatac
     7621 agagcatttg aagtagaata caggggagca atcccctttt tgctatcaag tcaatagttt
     7681 tgggtttttc catatagggg gaaataatga agcagttagc aatagaagca atcactaaac
     7741 caatgaagct gagaggaata tctaagggta tagccgagtt ggatggtcaa cgtttagaga
     7801 ttgatttgga tagtttaatg attgattttg gtggagagtc atttgaatta gacagaatag
     7861 caggaactaa gggaggtaat cgatattttt ttctttgccc tgattgtggt agacggtgca
     7921 ggttgcttta taaacggtat ttgtatttta gttgcggtac ttgtctagat atccacaagc
     7981 acaccctgaa ccgcagtaaa acggattgcc aatactactg ggagcttgca ctaaaagaag
     8041 ctagaaaggt agaaccaggg tggagtccta gacgtggcgg atatatgttt gatagttttc
     8101 ctgaaagacc aaaatacatg aaacagaaac gatattacaa gcattatcaa aagtttatta
     8161 actacaccag aaaaggggat agcttttggc tgaatggttt gagttctcta aggtaatttg
     8221 tagtagtgtg caatttacaa aacaaacaaa tactacatat tagacggttt gaatgttaaa
     8281 aaagctagat atatcaagga tttaattaag tatagcgttc caaaaaaagg gtgcagtaag
     8341 cgttccaaaa aagggtgagg taagggtgag gttgaatatt taaaaaaggc taagattagg
     8401 ctaaggtcac atacttcaaa atagcaaggt tttagcaagg tacagggctt ttttctagtg
     8461 atagaacccc aaggttacca caaggtatag ctactttttt agaaccatag aacggtaagg
     8521 ttacggtaag gtatcgaagt tttccaatgg taaggttttg gtaaggtagc atatataaaa
     8581 agtacaaggt tagtacaagg ttacatactt aaaaatacta aggttttaca aaggtagaga
     8641 ggtttaccga gggttagaaa aggtgagatt ttgcttaggt agtggcagta aatatgcctg
     8701 acaaatggca gggttatgtg tgttgaactc cgagcgaacg ccgagaaagt ggctatatcc
     8761 cccctatcat ttatcaagta gataagagca ataaaaaccc tccttaaccc cctgagaaac
     8821 gacaagcaga taagagaagt ttttagccct cttatttcgg atctactcct tgcagaatga
     8881 caagcagata aggacgacaa taagccttct taactgctta caaaacgata gggagttaag
     8941 acgaatttaa aagcccctta ttttcgtttt tctccttgtc aaacgggaag gaaataagac
     9001 aggaaataac atcgcttatt ttgattttgc tccctgaaaa actgtaagga gataaggaga
     9061 cttattagct tccttaattt gaattttctc cttacaaaat gacagggaaa taagaactaa
     9121 aataactccc cttattttcc taccaaatga taaggagata agagcagttt aaaagtctgt
     9181 tattttggat ttgttgctta caaaatgata agcagataag caaagaaata acttgcctta
     9241 attgcttgca gaatgacaag gaaataagag agataaaaac atcccttaat tgcttatcaa
     9301 atggtatgga gaatggactt gaacctagag cgaagctaga gaaaatgaaa aaaagccagc
     9361 acagggctga ctcctttatg aattattcca taaaactatt atatcacaaa ggaggccgag
     9421 gcatgacacc agagcaagta aaagaaaaac tagagggcgt taagtggata aacaaggaga
     9481 tcaaaggctt atatttggaa ttggaaaccc tggaaggtgg cattatcaaa aaaccaacac
     9541 taagccatag cagggtgcag acaagtagag agaataagac agagaacaat cttataagtg
     9601 ttctgaagct aaaagaggac actttacaga gaattgagcg acttactgaa gagagaatga
     9661 aaatatctga gctaatcgat aaactggcca atccgcttga gcgttctgtt ctaagacttt
     9721 tttacttgaa tgatcttgta gtttcggagg tggctgaaga aatagataga tcaaatacta
     9781 cagtatacat tataaggcaa aaagctatag aacacttaac aggtattgta aacggggatt
     9841 gatttaacct taccacggaa ttaggagagt atgggggagt cctaagccac atattcgccc
     9901 gtatttcgct ttgtttcgta ctggacaact aaaacaccat ggataaacta caaaccctca
     9961 gaaacgaatc tgggggcttt tgtgaggtca taatcttata aaccacgcgc ttcatggtat
    10021 aatatatcta tcagcaacca gcaataaaaa agcacgtttg accgtgctag tttcttgcct
    10081 gctgaactcg tcaaatctta gcccttttgt ggggcttttt tgctcccctt tttgcgtact
    10141 ttgggggagc aagtagtaaa aagtaatgtt agtaattttt attaaaaagc gcgtgatata
    10201 agggttagga agccctaagg aaacataaaa taagcgattg ttgattatac tacaaaatcg
    10261 gccttttggg taatagaatc tcactttttt tggtataatg gtaagcaata atggactaga
    10321 aagaacaaag atgcaagata aaattgtcat tcatggggcg cgtgcccata atttaaaaaa
    10381 tattgatgtg gagattccgc gagacaagtt ggttgtcgtg actggcttgt caggttcagg
    10441 gaagtccagt ctggcttttg atactctcta tgcggaggga caacgtcgct atgtagagag
    10501 tttgtctgcc tacgctcgtc agttcttggg aaatatggaa aaaccagatg tagatgccat
    10561 tgatggtctc agcccagcta tttccatcga ccagaaaacg actagtaaaa atcctcgctc
    10621 gacggtggga acgacgactg aaatcaatga ctatctgcgt ctcctctacg cacgtgtggg
    10681 gacgccttac tgtatcaacg gacatggagc tatcaatgct tcttctgtgg agcaaatcgt
    10741 tgataaggtt ttggagttac ctgaacgcca gcgcttgcag atcttggctc ctgtcatccg
    10801 caagaaaaaa ggccaacata agagtgttat cgagaaggtt cagaaagacg ggtatgttcg
    10861 tgttcgtgtg gatggggaag tctatgatgt gaccgaagtg ccagagttgt ctaagagcaa
    10921 gcaacacaat atcgatgtcg tggttgaccg tattgttatc aaggagggca ttcgtagccg
    10981 tctctttgat tccattgaag ctgcccttcg tatcgcagaa ggttatgtga ttatcgacac
    11041 catggacgac tctgagttgt tgttctctga gcattatgcc tgtccagttt gtggatttac
    11101 tgtcccagag ttagaacctc gtctcttctc tttcaatgct ccctttggct cttgtagtga
    11161 gtgtgacggc ttggggatta agctggaggt ggatactgat ttggtagtgc cagatgccag
    11221 caaaacctta catgagggag cgctggcacc ttggaatcct atctcatcca actactatcc
    11281 aaacatgtta gagcaggcca tgaaagtctt tggagtggct atggataagc cttttgagga
    11341 cctgtcagaa gaagataaga acttgattct ctatgggtca gatggtaagg aattccattt
    11401 ccactatgag aatgaatttg gtggcgtgcg cgatatcgac attccgtttg agggagttat
    11461 caataatatc aaacgtcgtt accatgaaac caatagcgat tacactcgca ctcagatgcg
    11521 tctctacatg aatgagctga cctgcggaac ctgtcaaggc tatcgtctca atgaccaggc
    11581 cttgtctgtc cgtgtgggcg gccagcaagg gccacatatt ggagaaatct cagacctgtc
    11641 tatcgctgac cacttggact tggtgagcca gttaactttg tctgaaaatg aagccatcat
    11701 tgctcgtccc attctcaagg aaatcaagga tcgtttgacc ttccttaata atgtgggtct
    11761 taactatcta accctgtcac gttcagcagg aaccctttca ggtggggaaa gtcagcgcat
    11821 tcgtttggca acccagattg gttccaacct atcaggcgtc ctctatatcc tagacgagcc
    11881 gtcaatcggt cttcaccaga gggacaatga ccgcctgatt gccagtctga aaaagatgcg
    11941 tgacttgggc aatactctta tcgtggtgga acacgacgaa gataccatgc gtgaagctga
    12001 ttatctgatt gacgttggtc ctggtgccgg tgtttttggt ggtgagattg ttgcagcagg
    12061 tacacctaag caagtggctc gcaacagcaa gtctatcaca ggtcagtact tgtcaggcaa
    12121 acgtgtcatc ccagtaccag aagagcgccg tgtcggaaat ggtcgtttta ttgaagtgat
    12181 aggagcgcgt gagaacaact tgcaaaatgt cacggctcgc tttccactag gaaaatttat
    12241 cgcagtgaca ggtgtgtcgg gttcagggaa atcgactcta atcaacagca ttctcaaaaa
    12301 agccattgcc cagaagctca accgtaattc agacaaacct ggtaagttta agactattac
    12361 agggattgag catgtcgacc gtttgattga tattgaccag agccctatcg gacggacgcc
    12421 gaggtctaac ccagctacct atacaggagt ttttgacgat atacgtgacc tttttgctca
    12481 gacaaatgaa gccaagattc gaggctacaa gaaggggcgc ttcagtttca acgtcaaggg
    12541 aggtcgctgt gaagcttgct caggtgacgg gattatcaag attgagatgc acttcttgcc
    12601 agatgtttat gtggcttgtg aagtttgcca cggaacccgc tataacagtg aaaccctaga
    12661 agttcactac aaggaaaaga atatttcgca ggtcttggat atgacggtca acgatgcggt
    12721 agaatttttc caacacattc cgaaaattca acgcaaactc cagaccatca aggatgtagg
    12781 tctaggctat gtgaccttgg gacagccagc taccaccctt tctgggggag aagcccaacg
    12841 tatgaagtta gctagcgaac tccacaaacg ctcgacagga aaatctttct acattctgga
    12901 tgagccgacg acagggcttc atacagagga cattgctcgc ctgctcaagg ttttagctcg
    12961 ctttgtagac gacggcaata cagtcctcgt catcgagcac aatctggatg ttatcaagac
    13021 ggcagaccat atcattgact tgggacctga gggcggtgtc ggtggtggaa ccatcatcgt
    13081 aacaggaact ccagaagaag tagctgccaa cgaagccagc tatacaggac actatttgaa
    13141 aggaaagtta catcatgaat aaacgcgtac aagcatttct agctaaaatg caagaaaaag
    13201 aactagatgg tatcatcatc aacaatctta aaaacgtcta ttatttgact ggtttttggg
    13261 gctcaaacgg aacagtcttt atcagccgtg accgtcaggt cttagtgaca gactctcgct
    13321 atatcatcgc agctaagcaa gaaaccagtg gttttgagat tgtggctgat cgtgatgaat
    13381 tggctgtcat tgcaggaatt gttaaggaca tgggcttgac tcgtatcggt tttgaagatg
    13441 agatttcagt gtcttattac caccgtatgc aggcagcttt tgcaggtttg aacttgcttc
    13501 cacaaactca gtttgtggaa ggtcttcgta tgattaagga tgaggcagag attgcagcga
    13561 ttcgcaaggc ttgttctatc tcagaccaag ctttccgcga tgcgcttgac tttatcaaac
    13621 caggaaaaac tgaaattgag attgccaact tccttgactt ccgcatgcgt gagttgggag
    13681 catctggctt atcttttgat acgatcctag ctagcggtat caattcttct aaaccccatg
    13741 cccatccaat gcacaaacca gtggagttgg gagaagccat taccatggac ttcggctgtc
    13801 tctatgacca ctatgtcagt gatatgaccc ggactatcta tctagggcat gttagcgatg
    13861 agcaggcaga gatttacaat acggttctaa aagctaacca agccttgatt gaccaagcta
    13921 aggcaggctt aggtttccgt gactttgaca aaatccctcg tgatattatc attgaggcag
    13981 gttatggcga ctactttact cacggcattg gccacggtat tggtctggat atccatgagg
    14041 aaccctactt tagccagact tctacagaaa ctattaagac aggtatggcc ttgaccgatg
    14101 aaccaggtat ctatatcgaa ggcaaatatg gcgttcgtat cgaggatgat atcctgatta
    14161 cagagacagg ttgtgaatta ttgaccctag ctccaaaaga gttgatagtc atttagttgt
    14221 ttgtcaagaa aaaagtacga aaatgacgaa aaaagtcaaa aaaaattaaa aataggtcgc
    14281 aagtcggatg ttttttatgg tataatagac taaactgata gtaacatgta gcgaaagggg
    14341 taggtacatg attaaaattt atacagtctc aagttgtact agctgtaaaa aagcaaaaac
    14401 ctggctcaat gcccaccagt taagttataa agaacaaaac cttggtaaag aaggaattac
    14461 gagagaagaa ttactggata ttctaaccaa aacagataac ggaatagcca gcatcgtttc
    14521 atctaaaaat cgctatgcca aagcccttgg agtggatatt gaagatttga gtgtcaatga
    14581 agttctcaat ctgattatgg aaacaccgag aattttaaag agcccaatcc ttgtagatga
    14641 aaaacgcctg caagttggct acaaggaaga cgatattcgt gccttcctac cacgctctgt
    14701 ccgtaatgta gaaaatgcag aagcacgttt gcgtgcagct ctataaacat caaggctggg
    14761 agcacttccc agtcttattc tattttaatt tcaaaaagaa agaagaaaga aatgaaaaaa
    14821 atagttcttg ttagtctagc tttccttttt gtcctggttg gttgcggaca gaaaaaagaa
    14881 actggaccag ctacaaaaac agaaaaagat acgcttcagt cggcattgcc agttattgaa
    14941 aatgctgaga agaatacagt tgtaactaag actttggtct tgcccaagtc agatgatggt
    15001 agccagcaga cacaaacaat tacttataaa gacaagactt ttttgagtct aactatccaa
    15061 caaaaacgtc cagtctctga tgagttgaag acttatattg accaacatgg agtggaggaa
    15121 actcaaaaag ctcttcttga agcggaggag aaggataagt ctatcattga agctcgtaaa
    15181 ttggcaggtt tcaaacttga aacaaaacta ttgagcgcaa cggaacttca aacaacgact
    15241 agttttgatt ttcaagttct ggatgtcaag aaggcttccc agttggaaca tctgaagaat
    15301 attggtttgg aaaatctttt gaaaaatgaa ccaagcaaat atatttcaga tagattggca
    15361 aatggcgcga cagaacaata gaaaatccaa ataaatagac tgcctgaatt gtagaaggta
    15421 aggaattatg ctataatggt actattctaa ggaaagaggg tgttagaatg ggatttactg
    15481 aagaaacagt acgttttaaa ttggacgatt ccaataaaaa agaaattagc gaaactttga
    15541 cagatgttta tgcttcgttg aatgataagg gttacaaccc aattaaccaa atcgtaggtt
    15601 acgtattgag tggagaccct gcctacgttc ctcgttataa taatgcacga aatcaaatcc
    15661 gtaagtatga gcgtgatgaa atcgttgagg aattggttcg ctactacctc aaaggacaag
    15721 gagtcgatct ataacctatg agaattatgg gattggacgt cggttcaaaa acggtagggg
    15781 tggcgattag cgatccgctt ggttttacag ctcaagggct tgaaatcatc cagataaatg
    15841 aagaacaagg ccaatttggt tttgaccgcg ttaaggaatt ggttgatact tacaaggtgg
    15901 aacgatttgt agtgggcttg cctaaaaaca tgaacaatac aagtggaccg cgcgtagaag
    15961 ctagtcaagc ctacggagca aagctagaag agttttttgg tttaccagta gactatcagg
    16021 atgaacgctt gacaacagtg gctgctgagc gcatgttgat tgaacaagca gatatcagtc
    16081 gcaataagcg caagaaagtc attgataagt tagcagctca gctgatttta caaaattatt
    16141 tagatagaaa attttaatat aaaggagagg ctatgtcaca cgatcataac cacgaccacg
    16201 aagaacgtga actaatcaca ctagtagatg agcaaggaaa tgaaaccttg tttgaaatcc
    16261 ttttgacaat tgatggaaaa gaagaatttg gtaaaaacta tgttcttcta gtaccagtta
    16321 acgcagaaga agacgaagac ggacaagttg aaatccaagc ttactcattc atcgaaaacg
    16381 aagatggaac agaaggcgaa ttgcaaccaa tcccagaaga ctcagaagac gaatggaaca
    16441 tgattgaaga agtcttcaac agctttatgg aggagtgata cagtctggga gactgtatca
    16501 gcccaactcc cagaaatggg gaagagttgg aacgtccagt ggatgttttt agcccagttc
    16561 ctttaaataa gagagaactg gtaggttcgg ggggacgttt ttagcccagc tcctttaaat
    16621 aagatagagc tggtaagtct gggagactgt atcagcccaa ctcccagaaa tggggaagag
    16681 ttggaacgtc cagtgaacat ttttacccca actcttagaa attgagaaga gttggaacat
    16741 ctagtggatg tttttagccc atattcacat tcatagtggc tagttattag ctaaccaggt
    16801 aagaggtcgg gacgaaaatc ctggccttgt ttttattagt aaccatactt taatgcgata
    16861 attgattgga cttttgtcaa ggagatgtgt atgtttgaag tagaagaatg gctccatagt
    16921 cggattggtt taaattttcg atcaggtttg ggccgaatgc agcaagcggt ggatttgtta
    16981 ggaaatcctg agcagtctta ccctattatc cacgtaacag ggactaatgg gaaaggatct
    17041 accattgctt ttatgaggga attatttatg gggcatggca aaaaagttgc gacctttacc
    17101 tcccctcata tcgtatccat caatgaccga atttgcatta atgggcaacc tatagcagac
    17161 gcagacttta tccgtttggc tgatcaggtc aaggagatgg agaaaacgct tctgcaaact
    17221 cctgaccagt tgtccttttt tgaattgctg accttggttg cttttcttta ttttagggag
    17281 caagaggtgg atttggtttt attagaagtg ggaattggtg gcttacttga cacgaccaat
    17341 gtggtaactg gagagcttgc tgtcatcacc tccattgggc ttgaccatca agaaaccttg
    17401 ggtgatagtc tagaagcaat tgcagagcag aaagctggta ttttcaaggc tggtaagaag
    17461 gcagtgattg cgaaattgcc tccagaagct aggcttatct gtcagaaaaa agccgaatct
    17521 ttagctgttg acctttatca ggcaggtcaa gatttttcag tgctgaatgg tgatttttca
    17581 agctctttac taaatctttc acagctgaac ataggcttag aaggagccta tcagcaggag
    17641 aatgcggcct tggcgttgca aacttttctt ctttttatgg gagaaagaaa ggaagctatt
    17701 gatgaacagg ctgtaagaaa gaccttggaa caaacccatt gggctggtcg cttggagcgt
    17761 attcgcccac agatttactt ggatggtgct cataacctcc ctgccttgac tcgcttggtt
    17821 gagtttatca aagaaaaaga gcaggaaggc tatcgacctc aaatcctctt tggatccttg
    17881 aaacgtaagg attatcaagg gatgttgggt tatctgactg aaaatttacc tcaagtggaa
    17941 ctcaaaatga ccggctttga ctatcaaggg tctttggacg agacagatgt aacaggttac
    18001 catgtaattc cctcttaccg agaatttatc agcagtttcg aagaaagggc agacgctcaa
    18061 gacttgctgt tcgttacagg gtctctctat tttatctcag aagtacgggg ctacctgctg
    18121 gaccgtgagc agataaattg accccctttt ttgaacttgt tatactaggg gaattgctgg
    18181 ttagagaaaa tttctctaaa ttggtagcag aaaggaaatt catcatgaaa ttaaaaagat
    18241 tcacactttc tcttgcttct ctagcaagtt ttagtctctt agtagcttgt tcacaaagag
    18301 ctcaacaggt tcaacagcct gttgctcagc aggtccaaca gcctgctcaa cagaatacca
    18361 atactgcaaa tgcaggaggt aaccaaaatc aagcggctcc agtacaaaac caacctgttg
    18421 ctcaaccgac cgatattgat gggacttata ctggtcagga tgacggagac cgtatcactt
    18481 tagtggtaac tggaacgact ggtacatgga ctgagctcga atctgacggg gatcagaaag
    18541 tcaaacaggt tacatttgat tcagcaaatc aacgcatgat tattggcgat gatgtcaaaa
    18601 tttacactgt aaacggtaat caaatcgtcg tagatgatat ggatagagac ccatcggacc
    18661 aaatcgtttt aactaaataa gactatgcaa gtagaaaggg ctggattttt cagccttttt
    18721 acttttacct agaaaataat cataaatact tgccttctac cgaatacctg cgttatacta
    18781 gtatcaatta cttgttttta gcattcaaac tatcaaggag gggatatgaa atataggaaa
    18841 tttcaattat tgatgtccaa gtatggcttt agtctttcga ttatgctact tgaactttgt
    18901 cttgtttttg gtctctttct ttatttagga cgcatggctc ccattttatg gattactgtc
    18961 ctcattctac tgagtatcat cacaatcatt tcgatagtca accgtaatac gactcctgag
    19021 aataaggtaa cctggttgtt agtagccttt gtgccagtat ttggtccctt gctctatctg
    19081 atgtttggtg aaaggcgatt gtccaaaaaa gaaatcaaac aactgaagaa gctaggctct
    19141 atgcatttcc aagaagcaaa tagccagcta ctaaaagaga aattaaaaga aagtgacaag
    19201 gcagcttatg gagtcatcaa gtccttattg agtatggata ccaatgctga catctatgat
    19261 caaactgcct ctacattttt tcctaacgga gaagctatgt ggaaaaagat ggtagaagat
    19321 cttaaaaagg ctgagaaatt tattttcttg gaatattaca ttatagaaga aggtttgatg
    19381 tggaatcgca tactagatat actagagcaa aaggtagctc agggtgtaga ggttaagatg
    19441 ctctatgatg atatcggctg tatggctact ttaacaggag attatgcaca tcgacttcgt
    19501 cagctgggca tcgaggccca taaattcaat aaagttattc ctcgtttgac agtggcttat
    19561 aataacagag atcatagaaa aatattgatt gttgatggtc agatagccta tactggtggg
    19621 gtcaatctgg cagatgagta cattaaccac gtcgagagat ttggttattg gaaggatagt
    19681 ggaattcgct tagacggact agcagtaaaa gccctgacac gcttattttt gaccacttgg
    19741 tacattaatc gaggagaaat tagtgatttt gatcaatatc atttagaaaa tcattctatc
    19801 ccgagtgacg gtttaaccat tccatacgga agtggaccca agccaatttt tcgagcgcag
    19861 gtagggaaaa aagtttatca gagtttaatc aatcaagcaa cggaatcggt ctatattacg
    19921 acaccttatt tgattataga ttatgattta acagagacaa tcaaaaatgc agctatgaga
    19981 ggggtcgatg ttcgaattat caccccttac ataccagata agaagttcat tcagttagtc
    20041 acgagaggag cttatcccga ccttctttct gctggtgttc ggatttatga gtatagtcca
    20101 ggttttattc atagtaagca gatgttggta gacgaagatt ttgcggtggt ggggacaatc
    20161 aatctcgact accggagctt ggtacaccat tatgaaaatg cagtcttact ctataaaact
    20221 ccttctataa gggaaatcgc ccgagatttt cgaaatatat ttgcagattc tcaggaagtc
    20281 tatcctcatt ctatcaaaac gagctggtat caaaagcttg taaaagaaat cgcccagcta
    20341 ttcgccccta tcttataaga aatctagaac ttaggataca aaccagacct atttttcttg
    20401 tcttttacga atagaaagaa agaggagggg aattcatgct aactaaggaa gaagtgaatg
    20461 atatgattga gttcaaacag acacatttac accgctttcg ggaaattgaa acctttgtga
    20521 aactatgcaa aaaatcactc aaacagccat cgcaagctga caatcctagg actttttgat
    20581 atactaagac agataaattg aacaggagaa acgatatgag tgtgtatggt agagtagaag
    20641 aagttcataa ggagaatcgt gaacctctag aataccaaat cgaacaagaa tcgcatcatc
    20701 gtgaatcaag tcgtcttcct ttagtgaaaa ttttactatg gagtacgctt gtaacgggga
    20761 taactctagg agtaccgcta ttactcgact taatgagtgc acaagaagtg caggattttt
    20821 atgcaggttg ggcgcttcat cagacaggga agatttacag cgactattat ggaagtcaag
    20881 gtttgcttta ttatttgctg acttacgtga gtcagggcgg atttttcttt gccatctttg
    20941 agtggttagc cttggtagca ggcggatttt tcctttttag atcagcggac accttgacag
    21001 agcaaggaga ccaagctgga catctggtga ctatttttta catgctagtt acaggtcttg
    21061 cttttggtgg aggctatgcg actcttttag cgcttccttt cttattcgca gcctttagtt
    21121 tagttgcggc ttacctaagc aatccaagcc atgataaggg atttgtacgg attgggctag
    21181 ctttggcagg cggatttttc tttgctccct tatcatcgct cctgtttatt gctgtagtga
    21241 gtttaggctt gttggtcttt aaccttgggc atagacgctt tgcgcatggg ttttatcagt
    21301 ttcttgcagt ggctttaggt ttttcacttg tcttttatcc aactgcctac tatagtgctg
    21361 caacaggaag ttttggggat gcgattagtg gtattcgtta tcctattgac agtattcgct
    21421 ttgattttac ttctaaaatt ttagagaata tgttttttta tggcttgttg tcccttggtt
    21481 tgggatttgt ggtttgtatc tttttaggtc tctttcaatc caaacccttt aaactatacg
    21541 tcatttcggt tcctgcaagt cttgtggtaa ttttaggttt gattttactt ttcttttcac
    21601 aagagcctct gcacgcttct tatttgatgg tcgtcttccc tgttttccta cttttattgg
    21661 taaccaatat taagagtcaa cagagggggc gtagtgctag aagaagccga agagaaacgc
    21721 cagttagcct atggagtcgt ttcttcaaag gaaatctata tctgctagtt tttgggtttg
    21781 tctatctttt gtctgttcct tttttgatga agtttgtcct ttatccagta ccttatcaag
    21841 aacgtaatcg tcttgctgat ttggtaaaag aggagacaaa tacggaagat gctatctatg
    21901 catgggatga tactgcgact ctttatcgta agagtgagcg cttgtcgcca tcggcgattt
    21961 tgtccccgtt gcactataca gcaactgagg aaaatcgtaa taagttactt aatgacttga
    22021 aagaaaaaca acctaaggtg attgtggtaa atgataaggt ggtagtctgg tctgaagtgg
    22081 aaacactctt aaaagaaaat taccaacaag taaaga
//
LOCUS       DAVLTM010000022        22040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLTM010000022 DAVLTM010000000
VERSION     DAVLTM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.022"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            93..2315
                     /gene="recJ"
                     /locus_tag="VYN16_000739"
     CDS             93..2315
                     /gene="recJ"
                     /locus_tag="VYN16_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1403303.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIGANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYRFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2490..4151
                     /locus_tag="VYN16_000740"
     CDS             2490..4151
                     /locus_tag="VYN16_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1403304.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMVVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4220..4999
                     /locus_tag="VYN16_000741"
     CDS             4220..4999
                     /locus_tag="VYN16_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1403305.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQDFSPESQNLG
                     TPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5111..6331
                     /gene="murM"
                     /locus_tag="VYN16_000742"
     CDS             5111..6331
                     /gene="murM"
                     /locus_tag="VYN16_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1403306.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6336..7568
                     /gene="murN"
                     /locus_tag="VYN16_000743"
     CDS             6336..7568
                     /gene="murN"
                     /locus_tag="VYN16_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1403307.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7647..8348
                     /locus_tag="VYN16_000744"
     CDS             7647..8348
                     /locus_tag="VYN16_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1403308.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQVSARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8388..10232
                     /gene="uvrC"
                     /locus_tag="VYN16_000745"
     CDS             8388..10232
                     /gene="uvrC"
                     /locus_tag="VYN16_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1403309.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNKP"
     gene            10222..11055
                     /locus_tag="VYN16_000746"
     CDS             10222..11055
                     /locus_tag="VYN16_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1403310.1"
                     /translation="MNHKIAILSDIHGNVTALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELEFAKSKGLPFIEMYEELRREDNYQRHNLELLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11237..12037)
                     /locus_tag="VYN16_000747"
     CDS             complement(11237..12037)
                     /locus_tag="VYN16_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1403311.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12208..12813
                     /locus_tag="VYN16_000748"
     CDS             12208..12813
                     /locus_tag="VYN16_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1403312.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12826..14226
                     /gene="pepV"
                     /locus_tag="VYN16_000749"
     CDS             12826..14226
                     /gene="pepV"
                     /locus_tag="VYN16_000749"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1403313.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14280..14858
                     /locus_tag="VYN16_000750"
     CDS             14280..14858
                     /locus_tag="VYN16_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1403314.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15018..15170
                     /locus_tag="VYN16_000751"
     CDS             15018..15170
                     /locus_tag="VYN16_000751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1403315.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15213..15305
                     /locus_tag="VYN16_000752"
     CDS             15213..15305
                     /locus_tag="VYN16_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1403316.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15477..16802
                     /gene="brnQ"
                     /locus_tag="VYN16_000753"
     CDS             15477..16802
                     /gene="brnQ"
                     /locus_tag="VYN16_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1403317.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16900..17937
                     /locus_tag="VYN16_000754"
     CDS             16900..17937
                     /locus_tag="VYN16_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1403318.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQKNEEHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVTSTGKTVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDEKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIDLPMTTHFAFSVFEE
                     VGHGANSNIPEEVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSEAVS"
     gene            17939..18400
                     /locus_tag="VYN16_000755"
     CDS             17939..18400
                     /locus_tag="VYN16_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1403319.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18418..19134
                     /locus_tag="VYN16_000756"
     CDS             18418..19134
                     /locus_tag="VYN16_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1403320.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19257..19682
                     /gene="rplK"
                     /locus_tag="VYN16_000757"
     CDS             19257..19682
                     /gene="rplK"
                     /locus_tag="VYN16_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1403321.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVASVTR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVVD"
     gene            19890..20579
                     /gene="rplA"
                     /locus_tag="VYN16_000758"
     CDS             19890..20579
                     /gene="rplA"
                     /locus_tag="VYN16_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1403322.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            20957..21094
                     /locus_tag="VYN16_000759"
                     /pseudo
     CDS             20957..21094
                     /locus_tag="VYN16_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(21342..21416)
                     /locus_tag="VYN16_000760"
                     /pseudo
     CDS             complement(21342..21416)
                     /locus_tag="VYN16_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21430..21792
                     /locus_tag="VYN16_000761"
     CDS             21430..21792
                     /locus_tag="VYN16_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1403323.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6620 a         4056 c         5079 g         6285 t
ORIGIN      
        1 taaaatgtta taaaaaagca atctggatag agaaaacgtc taaatcatgt tataatgaag
       61 caatagaatt cttagaaaga gtggatgtct ttttgataac acctacttat gaatggcagt
      121 ttgccctgca ggtagaagat gcggatttta caaagatagc caagaaggct ggactgggtc
      181 ctgaggtggc tcggttattg tttgagagag ggattcagga ccaagaaagt ctgaagaagt
      241 ttttagaacc ttccttggag gacttacatg atgcttatct gctccatgat atggacaagg
      301 cagtggagcg gattcgtcag gctattgaag aaggggaaaa tattctcgtt tatggagact
      361 atgatgcgga tggcatgact tcggcttcta ttgtgaagga aagtttggaa caacttggtg
      421 ctgagtgccg agtttacctg ccaaatcgtt ttaccgatgg ctatggccct aatgctagtg
      481 tttataaata ctttatcgag caagaaggga tttccttgat tgtgacggtg gacaatgggg
      541 ttgctggtca tgaggctatt gcattggctc agtctatggg agtagatgtc attgtcacag
      601 accatcattc catgcctgaa accctgccag atgcttatgc tattgtccat cctgaacatc
      661 cagatgcgga ttatcctttt aaatatttgg ctggttgtgg agttgctttc aagttggctt
      721 gtgccctgtt agaagaagtg caagtggaat tgcttgattt ggtcgctatt ggaactattg
      781 cagatatggt gagtctgacg gatgaaaatc gtatcttagt tcaatatggt ctggaaatgt
      841 tgggtcatac ccagcgcatt ggtctgcaag aaatgctgga catggctggg attggtgcca
      901 acgaagtaac agaagaaacg gttggtttcc agattgctcc tcgtttgaat gccttgggtc
      961 gcttggatga tcccaatcct gccattgatt tgttgactgg atttgatgat gaggaagcgc
     1021 atgagattgc ccttatgatt caccagaaaa acgaagagcg caaggaaatc gttcagtcta
     1081 tctatgaaga agccaagacc atggtggatc ctgagaagaa ggttcaggtc ttggccaagg
     1141 aaggctggaa tcctggggtt ctaggaatcg tggctggtcg tttattggaa gaattgggac
     1201 agacagtcat tgttcttaat atagaagacg gtcgtgccaa gggcagtgct cgtagtgtgg
     1261 aagcggtcga tatttttgaa gctctggatc cccatcgaga cctcttcatc gcctttggag
     1321 gtcatgcagg tgcagcgggt atgacgttgg aagttgagca actctcagat ttatctcagg
     1381 ttttggaaga ttatgttcgt gaaaaaggtg cagatgctgg tggcaagaat aagttaaacc
     1441 tagatgaaga gttggatttg gaggcactta gcttggaaac ggtcaaaagt tttgaacgtt
     1501 tagctccttt tggaatggat aatcagaaac ctatttttta tatcaagaat tttcaggtcg
     1561 aaagtgctcg tactatgggg gcaggtaatg cccatctaaa gctgaaaatt tccaagggtg
     1621 aggcgagttt tgaagtggta gcctttggtc aaggcagatg ggcgacagag ttttctcaaa
     1681 ccaagaatct agagttagcg gttaaattgt ctgtcaacca atggaatggc caaactgccc
     1741 tccagttgat gatggtggat gcgcgagtgg aaggtgttca actttttaac attcgtggaa
     1801 aaaatgcagt cttgccagaa ggtgttccag tcttggattt tcctggagaa ctgccaaatc
     1861 ttgcggctag tgaagctgtt gtcgtaaaaa acattccaga ggatattact cagctgaaga
     1921 ccatttttca ggaacagcat ttctctgctg tctatttcaa aaatgatatt gacaaggctt
     1981 attatctgac aggttatggg actagagatc agtttgccaa attgtacaag actatttacc
     2041 ggttcccaga gtttgatatt cgctacaagc tgaaagattt ggctgcatat cttaatattc
     2101 aacaaatctt gctggtcaag atgattcaag tatttgaaga actaggcttt gtgacgataa
     2161 aagatggtgt gatgacagtc aataaagagg cgccaaagcg ggagatagga gaaagtcaaa
     2221 tttaccaaaa tctcaaacaa accgttaaag accaagaaat gatggcgctg ggtacggtgc
     2281 aagaaattta tgattttttg atggaaaaag agtagaagtt aggaaagagt tgggaaatca
     2341 actctttttt gaaaacagac cttcattttg aaaatcatca aaaaaatggt ataatggtag
     2401 gaaaagattc ggctgaaagt atcagaactt ttagaataag agggtagaat tgccctataa
     2461 tcaagataaa ctaagatttt ggaggaaaaa tgagtaatat cagtttaaca acacttggtg
     2521 gtgtgcgtga gaatggaaaa aatatgtaca ttgctgaaat tggagagtcc atttttgttt
     2581 tgaatgtagg gttaaaatat cctgaaaatg aacaattagg ggtcgatgtg gtgattccaa
     2641 acatggatta cctttttgaa aatagcgacc gtattgctgg ggttttcttg acccacgggc
     2701 atgcggatgc cattggtgct ctaccgtatc tcttggcaga ggctaaagtt cctgtatttg
     2761 ggtctgagtt gaccattgag ttggcaaagc tctttgtcaa aggaaatgat gccgttaaga
     2821 aatttaatga tttccatgtc attgatgaga atacggagat tgattttggt gggacagtgg
     2881 tttccttctt ccctacgact tactccgttc cagagagtct gggaattgtc ttgaagacat
     2941 cggaaggaag catcgtttat acaggtgact tcaaatttga ccaaacggct agtgaatctt
     3001 atgcaactga ttttgctcgt ttggcagaga ttggtcgtga cggcgtcctg gctctcctca
     3061 gtgattcggc caatgcagac agcaatattc aggtggctag tgaaagtgaa gttagggatg
     3121 aaattaccca aactattgct gactgggaag gtcgtatcat cgttgcagct gtttccagta
     3181 atctttctcg tattcagcag atttttgacg ctgcggataa aacaggtcga cgtatcgtct
     3241 tgacaggatt tgatattgaa aatatcgtcc gcacagcgat tcgtcttaag aagttgtctt
     3301 tagccaacga aattctcttg attaagccta aagatatgtc tcgatttgaa gaccatgagt
     3361 tgattattct tgagacaggt cgtatgggtg aacctatcaa tggacttcgt aagatgtcga
     3421 ttggtcgcca tcgttatgta gaaatcaagg atggggacct ggtctatatt gctacggctc
     3481 cgtctattgc taaagaagcc tttgttgcgc gtgtggaaaa tatgatttat caggcaggtg
     3541 gggttgtcaa attgattacc caaagtttac atgtatcagg gcacggaaat gtgcgtgatt
     3601 tgcagctgat gatcaatctt ttgcaaccta agtacctctt ccctgtccaa ggggagtatc
     3661 gtgagttgga tgctcacgct aaggctgcca tggtagttgg gatgttgcca gaacgcatct
     3721 tcattcctaa aaaggggacg accatggctt acgagaatgg agactttgtt ccagctggat
     3781 cggtttcagc aggagatatc ttgattgatg ggaatgccat tggtgatgtt ggaaatgttg
     3841 ttcttcgtga ccgtaaggtc ttgtcagagg atggaatttt catcgtggct attacagtca
     3901 accgtcgtga gaagaaaatt gtggctaggg ctcgtgttca cacgcgtgga tttgtttatc
     3961 tcaagaagag tcgcgatatt ctccgtgaaa gttcagaatt gattaaccaa acggtagaag
     4021 agtatcttca aggagatgac tttgactggg cagatcttaa agggaaggtt cgagataatt
     4081 tgaccaagta tctctttgac caaaccaagc gtcgcccagc cattttacca gtagtcatgg
     4141 aagcaaaata atcgttaaaa taaacagaga gaaagtcgag tttcggcttt ttcttataga
     4201 aaaatagaag gagaaaatca tggcagtaat gaaaatcgag tattactcac aagtattgga
     4261 tatggagtgg ggggtgaatg tcctctaccc tgatgccaat cgagtggaag aaccagagtg
     4321 tgaagatatt cccgtcttgt accttttgca cgggatgtct ggcaatcata atagttggct
     4381 taagcggacc aatgtagaac gcttgcttcg aggaactaat ctcatcgttg ttatgcccaa
     4441 taccagcaat ggttggtaca ccgataccca gtatggtttt gactactaca cggctctagc
     4501 agaggaattg ccacaggttc tgaaacgctt cttccctaat atgactagca agcgtgaaaa
     4561 gacctttatc gctggtcttt ctatgggagg ctacggctgt ttcaaactgg ctcttgcgac
     4621 aaatcgtttt tctcatgcag ctagcttttc aggtgcattg agctttcaag atttttctcc
     4681 tgagagtcaa aatctgggaa cgccagccta ctggagaggt gtttttggag agattaaaga
     4741 ctggacaact agtccctatt ctcttgaaag tctggctaaa aaatcggata aaaagaccaa
     4801 actttgggcg tggtgtggcg aacaggattt cttgtacgaa gccaataatc ttgcagtgaa
     4861 aaatctcaaa aaactaggtt ttgatgtgac ctatagccat agcgctggaa ctcacgagtg
     4921 gtactactgg gaaaaacaat tggaagtttt tttaacaacc ctaccaattg atttcaaatt
     4981 agaagagaga ctgacttagt ttgaacttca gcatagggga agtagaacta aaataaaata
     5041 tgttttcact agacttttca aacgaaagta gtagaatagt aataaaatac tggaggaaag
     5101 agagtaggaa atgtaccgtt atcaaattgg cattcccaca ttagaatatg atcagtttgt
     5161 caaagaacat gaattagcca atgtattaca aagtagtgct tgggaggaag ttaagtctaa
     5221 ttggcaacat gagaagtttg gtgtttacag ggaagaaaaa ttactggcga cagctagtat
     5281 tttgattaga actcttccgc taggctataa aatgttttac atcccaagag gacctatatt
     5341 ggattatggg gataaagaac tcttgaattt tgccattcag tctattaagt cctatgctcg
     5401 cagtaagaga gcggtttttg tgacttttga cccaagtatt tgcctatctc aaagtttaat
     5461 caatcaggaa aagacagaat ttcctgaaaa tctggctatt attgatagtt tgcaacaaat
     5521 gggagtaagg tggtcaggaa aaacggagga aatgggagac accattcaac ctcgtattca
     5581 ggcgaaaata tacaaggaaa attttgaaga agataaactt tccaagtcaa caaaacaggc
     5641 tattcgaaca gcacgaaaca aagggcttga gattcaatat ggtggactgg aactattaga
     5701 ttcattttcg gagttgatga aaaaaactga gaagcgaaaa gagattcatt tgaggaatga
     5761 agcctattat aaaaaattgt tagataattt taaggacaag gcctatatca ccttggccac
     5821 cttggatgtt tctaaacgtt cgcaagagtt agaagaacag ttagcgaaaa atagagcctt
     5881 ggaagagacc tttactgagt cgactcgaat ttcaaaagta gaagcgcaga agaaggaaaa
     5941 agaacgtttg ttagaggaat tgaccttctt gcaggaatat atagatgtag gtcaagcgag
     6001 agttccttta gcggctactt tgagtttgga atttggtact acctctgtca atatatatgc
     6061 tggtatggat gatgatttta aacgttacaa tgcaccaatt ttaacatggt atgaaacggc
     6121 tcgctatgcc tttgagcgag gtatggtctg gcaaaattta ggtggtgttg aaaactctct
     6181 caatggtgga ctttatcatt ttaaggaaaa atttaatcca acgattgaag aatacttggg
     6241 tgaatttaca atgcccactc atcctctcta tcctctgtta agacttgctc ttgatttccg
     6301 taaaacatta agaaaaaaac atagaaagta agtatatggc actaacaaca ctcacgaaag
     6361 aagagtttca gacttattct gatcaggttt cttctcgttc ctttatgcaa tctgtccaga
     6421 tgggggattt gctagaaaaa agaggggctc gaattgttta tcttgctttg aaacaagaag
     6481 gagaaattca agttgcagct ctggtttata gcttgcccat gctgggtggt ctgcatatgg
     6541 aactcaattc ggggccgatt tatacccaac aagatgctct tccagttttt tatgcagagt
     6601 taaaagaata tgccaagcaa aatggtgtat tagagttgct tgtaaaacct tatgaaactt
     6661 atcaaacttt tgatagccaa ggtaatccaa tagatgctga gaaaaaaagt attattcaag
     6721 gtttgactga tttaggttat caatttgatg gcttaacaac aggttaccca ggtggagaac
     6781 cagattggtt atactataaa gatttaactg aattaactga aaagagtttg cttaaaagtt
     6841 ttagcaaaaa gggtaaaccc ttggtgaaaa aggctgaaac ctttggcatt cggttgaaaa
     6901 agttaaaacg tgaagaacta tcgattttta agaatataac aaaagaaacc tctgaacgta
     6961 gagaatatag tgataaaagt ttagaatatt atgagcattt ttatgatact tttggagaac
     7021 aagcggagtt tctcatagca agcttgaatt tttcggacta tatgagcaaa ttgcaaggtg
     7081 aacaaagtaa actagaagaa aacttggaca agttgcgact tgatttgagt aaaaatcctc
     7141 attctgagaa aaaacaaaat caactgagag aatattctag tcaatttgaa acgtttgaag
     7201 ttcgaaaagc agaagcgcga gacttgattg aaaaatatgg agaagaagat attgttttag
     7261 ctgggagttt atttgtttat atgcctcagg aaacgactta tctctttagt ggttcctaca
     7321 ctgagtttaa taagttctat gcccctgcac tgcttcaaaa atatgttatg ttggaaagca
     7381 taaaacgtgg aatacctaaa tacaacttcc taggcattca agggattttt gatggaagtg
     7441 atggtgtttt gcgttttaaa cagaatttta atggctatat tgtacgcaaa gcaggtactt
     7501 tccgttacca tccatcgcct ttaaaataca aagctatcca gttactcaaa aaaatagtag
     7561 gacgttaaga tgaaaaagtc agtatttaga tttcttttag cttcttttag taaaataatt
     7621 tttatttgct agaaaggtgg agagacatgc gctggctttt tcgtttgata ggggctttct
     7681 tttcttttgt gtggcgtttg ttttggcgtc tggtttggat agttgtgctc ttatgtgtgc
     7741 ttgctttcgg acttctctgg tatctgaacg gggattttca aggagcgcta aagcaagcag
     7801 aacggtcagt aaaaattggt caacaaagta ttgaccaatg ggagaaaaca gggcaactgc
     7861 ctaagttaag ccagacagat agtcaccagc attctgaagg aaggtggcca caggtctctg
     7921 ctcgtattta cctggatccg cagatggatt cacgctttca agaggcttat ttagaagcaa
     7981 tccagaactg gaatcaaact ggtgctttta actttgaact cgtgactgag tctagtaagg
     8041 cggatattac ggctacggag atgaacgacg gaggcactcc tgtggcagga gaggcggaaa
     8101 gtcaaactaa tctcttaaca gggcaattct tgtccgtaac ggtgcggttg aatcattatt
     8161 atttgtccaa tccatactat ggctactcct atgaacgcct tgtccatacg gcagaacatg
     8221 agttaggtca tgcgattggc ttggaccata cagatgagaa gtctgtcatg caaccagcag
     8281 gttcctttta tggtatccag gaagaggatg ttgcaaacct ccgaaaaata tatgagacta
     8341 gtgagtaggg tactatcttt ccctactttt tttgctataa tggaactatg aacaacttga
     8401 ttaaatcaaa actagagctc ttgccgacca gccctggttg ctacattcat aaggataaaa
     8461 atggcaccat tatctatgta ggaaaggcta aaaatctgcg taatcgagta cggtcctatt
     8521 ttcgtggaag tcatgatacc aagacagagg ctctggtgtc tgaaattgtg gattttgaat
     8581 ttattgttac ggagtctaat attgaggcac ttctcctaga aatcaacctg atcaaggaaa
     8641 acaagcccaa gtacaatatc atgctcaagg atgacaagtc ctatcctttc atcaaaatca
     8701 ccaatgagcg ctatccacgc ttgattatca ctcgtcaggt caaaaaggac ggaggtcttt
     8761 attttggacc ttatcccgat gtgggggcag ccaatgaaat caagcggttg ctggatcgga
     8821 tattcccttt tcgtaagtgt accaacccgc cctctaaggt ctgtttttat taccatatcg
     8881 gccagtgtat ggctcacacc atctgtaaga aggatgaggc ttatttcaag tctatggccc
     8941 aggaggtttc tgattttctg aaagggcagg ataacaaaat cattgatgag ctaaagggca
     9001 aaatggcagc ggcagcccag actatggagt ttgaacgtgc ggcggaatac cgtgacctga
     9061 ttcaggctat tggaacgctt cgaaccaagc aacgggtcat ggctaaggat ctccaaaatc
     9121 gcgatgtctt tggctactat gtggataagg gctggatgtg tgtgcaggtt ttctttgttc
     9181 gtcagggcaa gctcatcgag cgcgatgtca atctcttccc ctacttcaat gatccagatg
     9241 aggatttctt gacctatgtg ggacaattct atcaagaaaa atctcaccta gttcccaatg
     9301 aggtactgat tccgcaggat attgacgaag aggctgtcaa ggccttggtg gattccaaga
     9361 ttcttaagcc tcaacgtgga gagaaaaagc agctggtcaa tttagctata aaaaatgccc
     9421 gtgttagcct agagcagaag ttcaacctgc tagagaaatc tgtcgagaag acccaaggag
     9481 ctattgaaaa tttaggacgc ttgcttcaaa tcccaacccc agttcgcatc gagtccttcg
     9541 ataactccaa catcatggga actagtcctg tttcagccat ggtggtcttt gtcaacggca
     9601 agcctagtaa gaaggattac cgtaagtata aaatcaagac cgtagtcggg ccagacgact
     9661 atgccagtat gcgagaggtc attcgcagac gctatggtcg agtacagcgt gaggctttga
     9721 ctcctccaga tttgattgtg attgatgggg ggcaaggtca agtcaatatt gctaagcaag
     9781 tcatccaaga agaactgggc ttggatattc caattgcagg tctgcaaaag aatgataagc
     9841 accaaaccca tgaattgctc tttggagatc cgctggaagt ggtggacttg tctcgcaatt
     9901 ctcaggaatt tttcctcctc caacgcatcc aagatgaggt gcaccgcttt gccatcacct
     9961 tccaccgcca actgcgctcc aaaaattctt tctcatctca attggatggg attgatggtc
    10021 tgggacctaa acgcaagcaa aatcttatga agcatttcaa gtctttgacc aaaatcaagg
    10081 aagccagtgt ggatgagatt gtcgaagttg gggtacctag agtcgttgca gaggctgtgc
    10141 aaagaaagtt gaacccgcag ggagaagcct tgcctcaagt agcagaagaa agagtagatt
    10201 accaaacgga aggaaaccac aataaaccat aaaatcgcaa ttttatcaga tattcatggc
    10261 aatgtgacgg cgctagaagc agtgattgca gatgctaaaa atcaaggagt cagtgaatac
    10321 tggcttctgg gagatatttt tcttcctggt ccaggcgcaa atgacttagt cgccctgcta
    10381 aaggaccttc ctatcacagc aagtgttcga ggcaattggg atgatcgtgt ccttgaggct
    10441 ttagatgggc aatatggctt agaagaccca caggaagttc agctcttgcg tatgacacag
    10501 tatttgatgg agcgaatgga tcctgcaacg attgtctggc tacgaagctt gcctttgctg
    10561 gaaaagaaag aaattgacgg attgcgcttt tctatctctc ataatttacc tgacaaaaac
    10621 tatggtggtg acttgctagt tgagaatgat acagagaaat ttgaccaact gctagatgcg
    10681 gaaacggacg tggcagttta tggtcatgtt cacaagcagt tgcttcgtta tggtagtcaa
    10741 gggcaacaaa tcatcaatcc agggtcgatt ggcatgccct attttaattg ggaggcgtta
    10801 aaaaatcacc gtgcccagta tgccgtgata gaggttgaag atggggaatt ggtaaatatc
    10861 ctatttcgta aagtcgctta tgattacgag gctgagttag aatttgccaa atccaagggg
    10921 cttcccttta tcgaaatgta tgaagaacta cgtcgtgaag ataactatca gaggcacaat
    10981 ctggaactat tagcaagctt aatagaaaag cataggtatg tagaggatgt gaagaatttt
    11041 tttgattttt tgtaagagtt tcctaaaata accaatgcaa actaaaaaag cgattggctg
    11101 atcccatcgc ttttagtata tcttatactc aatgaaaatc aaagagcaaa ctaggaagct
    11161 agccgtaggt tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag
    11221 agattttcga agagtgttat tgtaactgag attgatctgg gaggtaagaa ccacctagat
    11281 aggtattgct gagtttttca agggttccgt cttgatagag ttctttgagc gctttatcaa
    11341 attgctcttt aaactctttt tggtcgcttg agaaaatgat ataattgctg gggctatctg
    11401 cagaaggtaa atcaacgact gagaggtcta aaccacggtc cttgataatc ttttgaacgg
    11461 ataccttgtc aaaaactagg aaatcaaact ctccgttagc aaggtctagg attcgtttac
    11521 caatatcctc accagaaaaa ttaattgtag cgggattatc agtgtgtttc tgattccagt
    11581 tattgatgaa ttgagcgtta gaagttccgg tatcctcttg tgttgtttta ccagcgatct
    11641 ggtcaagaga agtcaaagga tttttcttgt tgctgacaag gacgagggga ttgttggaaa
    11701 ttggaagcga gtaaaggtat ttttcagcac gctcttttgt gtaactcaag ttattggccg
    11761 cagcctgata gtgaccagaa tcaagtcctg ggaagatact ttcccaggcg gttctttgaa
    11821 attgaatctc gtagtcgctg agtttttcat ctactgcctt tagaacttcg atatcgaagc
    11881 ctgtcagatt gcctttatct tcgtagtcaa atggtggcac gtcgccagct gtagcaagga
    11941 cgattgtctt ttgagcgcta gtctctttgg gtgtagcttg attcccacag gcaaccaaaa
    12001 atggtaggat agctagtaat aggctaaatt ttttcatact gtctccattc aaatgtaaag
    12061 tacttgctag tttatcagaa actttcctga taaaccaata tatatctttt atgtctgtga
    12121 tagaaaaact taatcatgaa aaatccctgc aagctctttc aaattatggt agaatggaag
    12181 cagtagaatt agaaaaggaa atcgattatg aaatttcttg aattaaataa aaaacgtcat
    12241 gcgaccaagc atttcactga taagcctgtt gatccaaaag atgtgcgtac ggctatcgaa
    12301 attgcaacct tggcgccaag cgcccacaac agccagcctt ggaaatttgt agttgttcgt
    12361 gagaaaaatg ctgaactggc aaagttggct tatggttcaa actttgagca ggtatcatca
    12421 gcacctgtaa ccattgccct atttacagat acagaccttg ccaaacgcgc tcgtaagatt
    12481 gcccgagttg gtggtgctaa caatttctca gaagaacaac ttcaatactt catgaaaaat
    12541 ttgccagctg aatttgcacg ctacaatgaa caacaagtca gcgactacct agcactcaat
    12601 gcaggtttgg ttgctatgaa cttggttctc gctcttacag accaaggaat tggttccaat
    12661 attatccttg gttttgacaa atcaaaagtc aatgaggttt tggaaattga agaccgtttc
    12721 cgcccagaac tcttgatcac agtgggttac acagacgaca aattggaacc aagctaccgc
    12781 ttgccagtag atgaaatcat cgaaaaaaga taggaagaag aaaaaatgat agcaattgat
    12841 tttacagcag aagtagaaaa acgcaaagaa gacctcttgg ctgacttgtt tagccttttg
    12901 gaaatcaact cagaacgtga tgatagcaag gctgatgccc aacatccatt tggacctggt
    12961 ccagtaaaag ccttggaaaa attccttgaa atcgcagacc gtgatggtta tccaactaaa
    13021 aatgttgata actatgcagg acattttgag tttggtgatg gagaagaagt tctcggaatc
    13081 tttgcccata tggacgtggt tccagctggt agcggttggg acacagaccc ttacacacca
    13141 actatcaaag atggtcgcct ttatgcgcgt ggtgcttcag atgacaaggg acctacaaca
    13201 gcttgctact atggtttgaa aatcatcaaa gaattgggcc ttccaacttc taagaaagtt
    13261 cgcttcatcg ttggaacaga cgaagaatca ggctgggcag atatggacta ctacttcgag
    13321 cacgtaggac tcgccaagcc agactttggt ttctcaccag atgctgaatt tccaatcatc
    13381 aatggtgaaa aaggaaacat cacggaatac ctccactttg caggtgaaaa tacaggtgca
    13441 gcccgtcttc acagctttac aggtggttta cgtgaaaata tggtaccaga atcagcaaca
    13501 gcagtcgttt caggtgactt gacagacttg caagctaaac tagatgcctt tgttgcagaa
    13561 cacaaactta gaggagaact ccaagaagaa gctggccaat acaaggtgac gatcattggt
    13621 aaatcagccc acggtgctat gcctgcttca ggtgtcaatg gcgcaactta ccttgccctc
    13681 ttcctcagcc agtttggctt tgctggtcca gccaaagact accttgacat cgcaggtaaa
    13741 attctcttga acgatcatga gggtgaaaat ctcaagattg ctcatgtgga tgaaaagatg
    13801 ggtgctcttt ctatgaatgc cggcgtcttc cgcttcgatg aaacaagtgc tgataatacc
    13861 attgccctta acatccgcta tccaaaagga acaagtccag aacaaatcaa gtcaatccta
    13921 gaaaacttgc cagttgcttc tgtcagtcta tcagaacatg gtcatacccc tcactatgtg
    13981 ccaatggaag atccacttgt gcaaaccttg ttgaatgtct atgaaaaaca aacaggccta
    14041 caaggtcatg aacaagtcat cggtggtgga acctttggtc gcttgttaga gcgcggagtt
    14101 gcctatggtg ctatgttccc agactcaatt gataccatgc accaagccaa tgaatttatt
    14161 gccttggacg atctcttccg agcagcagca atttatgccg aagctattta cgaattgatc
    14221 aaataaaacg atagaagtct gagatcttat gcttggactt ctttttggag ggaaagtaga
    14281 tgtctcaaat cgaaagaatc aaacaggcta tcatggcgga ttcacagaat gccagctata
    14341 cagagcgtgg cattgagcct ctctttgcag cgccaaaaac tgctcgcatc aatatcatcg
    14401 gtcaggctcc gggacttaaa actcaagaag caggccttta ctggaaagat aaaagtggtg
    14461 accgcttgcg ggactggcta ggtgtggatg aagatacctt ttacaattca ggttattttg
    14521 ctgttttgcc tatggatttc tactttccag gacatggcaa gtcgggtgat cttccgcctc
    14581 gtacaggttt tgcagaaaaa tggcatccgc agctcttaca ggaattgcct gatattcagt
    14641 taaccctctt gattgggcaa tatgcccaag cctactattt acaggagaaa atcagtggga
    14701 aggtaacgga gagggtgaaa cactataaag actatctgcc agcctatttt ccgctagttc
    14761 acccatcacc acgaaatcaa atctggatgg ccaaaaatcc ttggtttgag gcagaagtag
    14821 tgccagattt gaaaaaaaga attaaaacca ttttatagtc aatgaaaatc aaagagcaaa
    14881 ctaggaagct agtcgtaggc tgctcaaagt acagctttga ggttgcagat aaaactgacg
    14941 aagtcggtaa catacgcacg gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga
    15001 gtattagaag aaaaagaatg aaagaaatag cctttgacgc attttaccag ctttaccaaa
    15061 acgaccagct ttctctagtg gatgtgagag aagtggatga gtttgcagct cttcatttag
    15121 aaggtgccca caacctaccg cttagtcaat tggctgatag ttatgattaa ttggacaaag
    15181 atcgcttgca ttatattatt tgcaaatctg gaatgagatc ggcgcgtgct tgccaattcc
    15241 tgttagaaca aggttataat gttatcaatg tccagggtgg catgttagcc tttgaagaac
    15301 tttaaaattt tgcatttctc ctacttggtg tggactgggt aggagagttt ttatttttag
    15361 ataattctta tttttaagaa aattgaaaac atttaatatt tgcctcgtga tgcttttttc
    15421 agactcctaa tcgtggtata ctaggtcagt attttataaa tatgaaggag atttttatgg
    15481 ctaaaaaagg taccctaaca ggtttgctcc tgtttggaat attttttggt gcggggaact
    15541 tgatttttcc gccttctcta ggtgctctat ctggagaaca ttttcttcct gccatcgcag
    15601 gttttgtctt ttcaggcgtt ggtatcgccg tcttgaccct tattattgga acgctaaatc
    15661 ctaaaggata tatctacgag atttcaacga agatagcgcc ttggtttgcg actctttacc
    15721 tctcagttct ttacttgtca atcggtccat tctttgctac cccacgtact gctacaacag
    15781 cttacgaagt agggattagc ccccttttgt cggatgcaaa taaaggactt ggcttgattg
    15841 tatttacggt tctgtatttt gcggcagcct atttgatttc gcttaatcca tcaaaaatct
    15901 tagaccgcat tggacgtatt ttaacgccag tctttgcaat tttgattgtt atcttggtcg
    15961 ttctgggagc tatcaaatat ggtggaacaa gtcctcaagc tgcttcagct gcttatcaag
    16021 cttctgcctt tggtacaggt ttcctagaag gttacaatac cttggacgcc cttgcctcag
    16081 tggcctttag cgtaatcgca gttcaaacct tgaaacaact tggattttca agtaagaaag
    16141 aatacatttc aactatttgg gttgttggta tcgttgttgc ccttgccttc agcgctcttt
    16201 acatcggttt aggttttctt ggaaatcatt tcccagtacc agctgaagcg atgaagggtg
    16261 gaacaccagg tgtttacatc ttgtcacaag ccactcaaga aatctttggc tcaacagctc
    16321 aactcttcct tgcagctatg gttaccgtaa cctgcttcac aacgactgtt ggtttgattg
    16381 tgtcaacagc tgagttcttt aatgagcgct tcccacaaat cagctacaag gtttatgcga
    16441 cagcctttac cttgattgga tttgctattg ccaatttggg tcttgatgcg attatcaagt
    16501 actcaattcc agtactggtt atcttgtacc caatcacgat tgctatcgtt atgattgtca
    16561 ttgtcaacaa atttgttgcc ctttcaaaac caggtatgca gttgacaatt gctgtggtta
    16621 cagttattgc cattgcaagc gtactaggaa gctcgtttaa ggttgagttt cttgcaaatc
    16681 ttgttagcgt tcttcctttt gccaaggcat ctctcccatg gttggtgcca gccattgttg
    16741 gaatcttgct ctcattggtt ctaccaaaca agcaagaaag cgatgttttt gaaatggaat
    16801 aatcacttaa atcacttttg tagccaagtc tacaggagtg attttctttt tttatccgat
    16861 gataaatgtg ttataatagg tagcgaaaga ggtgaagaaa tgaatcaaac agtagaatat
    16921 atcaaagaac tgacagccat cgcatcgcca acgggcttca ctcgtgaaat ttcgaattat
    16981 ttagtcaaga ctctagaagg ttttggttac cagccagttc gcacagccaa gggtggtgtc
    17041 aatgtgacca tcaaaggtca aaaaaacgaa gaacatcgct atgtaactgc ccatgtggac
    17101 acactcggag ccattgttcg tgctgtcaaa cctgacggtc gtctcaaatt ggaccggatc
    17161 ggtggttttc cttggaacat gattgagggt gaaaactgta ccgttcatgt gactagtaca
    17221 ggtaaaacgg tatctggaac tatcctcatc caccagactt cttgccatgt ctacaaagat
    17281 gcaggaactg ctgaacgcac tcaggacaat atggaagttc gtttagacga aaaagtgacc
    17341 aatgaaaaag aaacacgtgc cttgggtatt gaggttggtg acttcatcag ctttgacccg
    17401 cgaactgtcg tgacagagac tggttttatc aagtctcgac atttggacga caaggtcagc
    17461 gcagcaattt tgctaaatct ccttcgagtt tataaagaag aaggaattga cttgccgatg
    17521 acgacacact ttgctttttc agtctttgaa gaggtgggcc atggtgctaa ctccaatatt
    17581 ccagaagagg tagtagaata tctggctgtg gatatgggag ctatgggcga tgaccaacag
    17641 acggatgagt acacagtatc tatctgtgta aaggatgctt ccggtcctta tcactatgac
    17701 ttccgtcaac acttagtagc tttggcgaaa gagcaagata ttccatttaa gctcgacatc
    17761 tatccatttt acggatcgga cgcttcagca gcgatgtcag caggtgcaga ggtcaagcat
    17821 gcacttcttg gagctggtat tgagtccagt cactcttatg agcgcaccca catcgattca
    17881 gtagtagcga ctgagcgtat ggttgatgct tatcttaaga gcgaggcggt atcatagaat
    17941 gtgcttaatc tgtcagagaa tcgaactcat taaagctggg caaaatccct actttgtaaa
    18001 agaactagaa acaggctatg ttgttattgg tgaccaccaa tactttaagg gctatacctt
    18061 atttttagca aaagatcatg tcacagaact ccatcatatg gaaacttctg taaaacttcg
    18121 ctttttagag gaaatgagtt tagtccaaga agcagtcgcc aaagcatttg aggctgaaaa
    18181 gatgaatatt gaactcctag gaaatggcga tgcccacgct cattggcatc tttttccccg
    18241 aagagcaggt gatatgaaga gtcacggttt gaatggccgt ggcccagtct ggtgggtgcc
    18301 ctgggaagaa atggcggcag aagattgcca agtacaatcg ccagagctgg aagagatgat
    18361 taaaatctta tctcatgaat tggagaagta tttggcctaa gagagaggaa gaaagaaatg
    18421 aaaaaaagat atgtcatttt atctggttta ctagcattga cccttgcagc ttgttcgcaa
    18481 gaaaaaacca aagttgaaga aaacactcaa aaaacggagc aaagcagtca accggaagga
    18541 actgtaggta gcaaatctca agcttctagt cagaaaaagg cagaagttag caataaaggg
    18601 agctattata gtatccaagg gaaatacgat gagattatcc tagctaataa acgttatcct
    18661 ttgtcaaaag attacaatcc aggagaaaat ccaacagcca aggcagagtt gctcaaacta
    18721 atcgcagcta tgcaggctga gggctatcca atcagtgacc aatacagtgg gtttaggagt
    18781 tatgaaactc aggctaagct ctaccaagac tatgtcaatc aagatggtaa ggaagcagca
    18841 gatcgctact cagcacgccc aggttacagt gaacaccaga cgggtcttgc ttttgatttg
    18901 attgggacag atggggattt agttactgaa gaaaaggcag cccagtggct cttggaccat
    18961 gcggctgact atggctttgt tgttcgctat cttaaaggca aggaaaagga gactggctac
    19021 atggcagaag aatggcatct ccgctacgtt ggaaaagaag ccaaagaaat tgctgcaagt
    19081 ggtctcagtt tggaagagta ctatggcttt gaaggcggcg attatgtcga ttaaaaatga
    19141 aagttttctc ttgcattttt agataaatag tgtataatgg aaaggtatgt gtaaagcata
    19201 cttgtgggag gtaaaaatct tacattaccg ccaaaaccac aaaggaggat ttagaaatgg
    19261 ctaaaaaagt cgaaaaactt gtaaaattgc aaatccctgc tggtaaagct acaccagctc
    19321 caccggttgg acctgctctt ggtcaagctg gtatcaacat catgggattc acaaaagagt
    19381 tcaacgctcg tacagctgat caagctggta tgatcattcc agttgttatc tcagtttacg
    19441 aagataaatc atttactttc atcactaaga caccaccagc tgctgttctt ttgaaaaaag
    19501 ctgcaggtgt tgaaaaagga tcaggtacac ctaacaaaac taaagttgct tcagttactc
    19561 gtgcgcaagt acaagaaatt gcagaaacta agatgccaga tttgaacgct gcaaaccttg
    19621 agtctgcaat gcgtatgatc gaaggtactg ctcgttctat gggattcact gttgttgact
    19681 aatcaataac acagtagaaa atctcacagc agtctattag ttgcttttca tactaggcaa
    19741 gtgactgagc cttgtacttg ggtacagcaa gggagcttaa ggccgtagga agagaaaaat
    19801 agtagactga aaacccgcaa gacttcatca tttcgagaag tgacgtggga gatgaaaatc
    19861 gattgaacca cttacaagga gaatagaaaa tggctaaaaa aagcaaacaa cttcgtgctg
    19921 ctcttgagaa aatcgacagc acaaaagcat acagtgtaga agaagctgta gcacttgcaa
    19981 aagaaactaa ctttgcaaaa tttgacgcaa ctgtagaagt tgcttacaac ttgaacatcg
    20041 acgttaaaaa agctgaccaa caaatccgtg gagcaatggt attgccaaac ggtactggta
    20101 aaacttcacg tgttcttgtt ttcgcacgtg gtgcaaaagc tgaagaagca aaagctgctg
    20161 gtgcagactt tgttggtgaa gatgaccttg ttgctaaaat caacgacggt tggttggact
    20221 tcgacgtagt tatcgctaca cctgatatga tggctcttgt tggacgtctt ggacgtgtcc
    20281 ttggaccacg taacttgatg ccaaacccta aaactggtac tgtaacaatg gatgttgcta
    20341 aagcagttga agagtctaaa ggtggtaaaa tcacttaccg tgctgaccgt gcaggtaacg
    20401 ttcaagcaat catcggtaaa gtatcatttg aagctgaaaa attggttgaa aactttaaag
    20461 ctttcaacga aacaatccaa aaagcaaaac cagctacagc taaaggaact tacgtaacaa
    20521 acttgactat cacaactact caaggtgttg gtatcaaagt tgacgtaaac tcactttaat
    20581 cagtagttaa agtaatgtaa aaaagttgaa gacgctatgt ctcaactttt tttgatgtac
    20641 gacgggcatg ttgtatagta gattgaaact agaatagtac acatctactt ctaaaacatt
    20701 gttagaaatt gatttgactg tcctgaacga ttcgtcctat tcttatttca ttttattata
    20761 tatttgaggt gtaagtcctc ggtgggcacc tactaccggt gaacccaata gcgattccca
    20821 agtctgacta tcgtgaggta gcggattaaa acggtctggg gatagaccgt tttaagtctg
    20881 acgctggaaa taagaatcgt cagaggaagg gatagcgaaa tcgtggctct acgaacagga
    20941 acgtgataat aaggcctata tagcggataa ggtatctggc tggggcgacc actatcaatt
    21001 agtatctcag aagtcggtac ttaaacgtgc tatatcaaaa ccagtccttg aaaaacgtgg
    21061 attggtttcg tgtttggatt attaccttga atgacatgcg ttaacagtta gttgaaccgc
    21121 cgtatgccga acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat
    21181 ttcgaaatct agtggaatga atctggaata gtccatcgag ctttctaata ctcttcgaaa
    21241 atctcttcaa accacgtcaa cgtcgccttg ccgtgcgtat ggttactgac ttcgtcagtt
    21301 ctatccacaa cctcaaaaca gtgttttgag ctgactacgt cagttccatc tacaacctca
    21361 aaacagtgtt ttgagcaacc tgaggctagt ttcctagttt gctctttggt tttcattgag
    21421 tataacacat tgttagaagt tggtttaaat ttcctaatca gtttgttcac atttaccttc
    21481 gatatattat atcccatagt taaggttggt catacagatg attatagtca tggagccgta
    21541 aaacttagtg tttctttagt tgacaaagat gccatgaaaa aaatatctgt aactgtaata
    21601 ggatattttg aaataaatat agatgaaaat atcaccgata ttctatacgt aaatggtact
    21661 gctattcttt atcattattt acgttctatt gtttcaatag tttcggcaat tgatagcagt
    21721 gaagcaatgt tgctacctac cattaatgtt ttagagttac tagataaatc tcaacctttt
    21781 gaagaagaat aatttattag ctcactaaat tgagggtaag gaaaagtaaa atcagtaaga
    21841 aaaatgtctt gcattataca gcaacctttt gggaatgagt agatggattg aataaaattt
    21901 tattaagagt ggatgattta tctgtagatt attattggac agttagtctt gaagtagtct
    21961 aagaattagg ttataatcag tagaatcctt gctaataatg aggaggttag tttatgtata
    22021 gtagactgaa tctaaaatag
//
LOCUS       DAVLTM010000023        19783 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLTM010000023 DAVLTM010000000
VERSION     DAVLTM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.023"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..1426
                     /locus_tag="VYN16_000762"
     CDS             <1..1426
                     /locus_tag="VYN16_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1403324.1"
                     /translation="FEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTN
                     GIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRII
                     SQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQ
                     LLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEG
                     IEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLS
                     DFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPK
                     FVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNF
                     DQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSSYDIIISNFIIPPIENKRLI
                     YSNNINTVSLIYLLNAMMFIRLDE"
     gene            1788..2789
                     /locus_tag="VYN16_000763"
     CDS             1788..2789
                     /locus_tag="VYN16_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1403325.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            2938..3855
                     /locus_tag="VYN16_000764"
     CDS             2938..3855
                     /locus_tag="VYN16_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1403326.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3866..4756
                     /locus_tag="VYN16_000765"
     CDS             3866..4756
                     /locus_tag="VYN16_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1403327.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4785..6401
                     /locus_tag="VYN16_000766"
     CDS             4785..6401
                     /locus_tag="VYN16_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1403328.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     SDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6411..7730
                     /locus_tag="VYN16_000767"
     CDS             6411..7730
                     /locus_tag="VYN16_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1403329.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7938..8010)
                     /locus_tag="VYN16_000768"
     tRNA            complement(7938..8010)
                     /locus_tag="VYN16_000768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7975..7977),aa:Lys,seq:ctt)
     gene            complement(8275..9546)
                     /locus_tag="VYN16_000769"
     CDS             complement(8275..9546)
                     /locus_tag="VYN16_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1403330.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            9844..10059
                     /locus_tag="VYN16_000770"
     CDS             9844..10059
                     /locus_tag="VYN16_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403331.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFWEDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10224..10403
                     /locus_tag="VYN16_000771"
     CDS             10224..10403
                     /locus_tag="VYN16_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403332.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10540..10719
                     /locus_tag="VYN16_000772"
     CDS             10540..10719
                     /locus_tag="VYN16_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1403333.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGK
                     LNKYTERGIRKQAGL"
     gene            10756..11208
                     /locus_tag="VYN16_000773"
     CDS             10756..11208
                     /locus_tag="VYN16_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1403334.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11499..11969
                     /locus_tag="VYN16_000774"
     CDS             11499..11969
                     /locus_tag="VYN16_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1403335.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11988..13091
                     /locus_tag="VYN16_000775"
     CDS             11988..13091
                     /locus_tag="VYN16_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1403336.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERATTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNFWFPRL"
     gene            13057..13485
                     /locus_tag="VYN16_000776"
     CDS             13057..13485
                     /locus_tag="VYN16_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1403337.1"
                     /translation="MELEISGFPGCKIALFCEDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13623..14573
                     /locus_tag="VYN16_000777"
     CDS             13623..14573
                     /locus_tag="VYN16_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1403338.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            14575..15318
                     /locus_tag="VYN16_000778"
     CDS             14575..15318
                     /locus_tag="VYN16_000778"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1403339.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15329..17125
                     /gene="pepF"
                     /locus_tag="VYN16_000779"
     CDS             15329..17125
                     /gene="pepF"
                     /locus_tag="VYN16_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1403340.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            17372..18163
                     /locus_tag="VYN16_000780"
     CDS             17372..18163
                     /locus_tag="VYN16_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1403341.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            18274..18942
                     /locus_tag="VYN16_000781"
     CDS             18274..18942
                     /locus_tag="VYN16_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1403342.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(19111..19200)
                     /locus_tag="VYN16_000782"
                     /pseudo
     CDS             complement(19111..19200)
                     /locus_tag="VYN16_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(19197..19523)
                     /gene="queF"
                     /locus_tag="VYN16_000783"
                     /pseudo
     CDS             complement(19197..19523)
                     /gene="queF"
                     /locus_tag="VYN16_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
     gene            complement(19518..19667)
                     /locus_tag="VYN16_000784"
                     /pseudo
     CDS             complement(19518..19667)
                     /locus_tag="VYN16_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
BASE COUNT         6021 a         3429 c         4326 g         6007 t
ORIGIN      
        1 atttgaacat aaacgttggt ttcatcgttc tgaactagca gagttattaa attgtacaga
       61 acgtgcagtc aaagatgatc tatcccatgt taaatctgct tttcctgact tgatttttca
      121 ttcttctact aatggtatac gcattattaa taccgatgat agtgatattg aaatggttta
      181 ccatcatttc tttaaacatt caactcattt ttcgatttta gaattcatct tctttaatga
      241 aggttgtcaa gctgagagta tttgtaaaga attttatatc agttcatctt cgctctatcg
      301 tattattagc caaatcaata aagtgattaa aaggcaattt caatttgaag tcagtctgac
      361 ccctgttcaa atcattggaa atgagagaga cattcgttac ttttttgcac aatatttttc
      421 agaaaaatat tatttcctag aatggccatt tgaaaatttt tcatcagagc cactatctca
      481 attgttagaa ttggtttata aggaaacaag ctttccaatg aatttgtcaa cgcatagaat
      541 gctaaagttg ctcctagtta cgaacctata tagaataaag tttggtcatt ttatggaagt
      601 agataaagat tcttttaacg accaaagttt ggatttttta atgcaggcag aaggaataga
      661 aggtgttgct cagagttttg aatcagaata caatatctct ttagatgaag aagttgtttg
      721 ccaattattt gtgtcttact ttcaaaaaat gtttttcata gatgaaagtc tctttatgaa
      781 atgcgtaaaa aaggatagtt atgttgaaaa atcttaccat ctattgagtg attttattga
      841 tcagatttca gtcaagtatc agattgaaat ggagaataag gataatctga tttggcatct
      901 gcataatacc gcacatctgt atcgtcaaga gttgtttact gagtttattt tatttgatca
      961 aaaagggaat acaatcagga actttcaaaa tatttttcca aaatttgttt cagatataaa
     1021 aaaagagctt tctcactatt tagagactct tgaggtttgt tctagttcaa tgatggtaaa
     1081 tcatctatct tataccttca tcactcatac caaacatttg gtgattaatt tgctacaaaa
     1141 tcagcctaaa ctaaaggttt tagttatgag taattttgat cagtatcatg caaaattcgt
     1201 tgcagagaca ctttcttatt actgtagcaa taattttgaa ctggaagttt ggactgaatt
     1261 agaattatca aaggaatctt tagaagattc atcttatgat atcattattt ctaattttat
     1321 tattcctcct attgaaaata agagactcat ctattcaaat aatataaaca cggtctcact
     1381 catatatttg ttaaatgcca tgatgtttat tcgattagat gagtaaccaa aaataacaaa
     1441 aaaacgctat ttcgcgtttg aactgtacct caaaagttag acagaaaaag tctaattttt
     1501 ggggtgtttt tgttaagaaa ataccttcaa tcatttaagc aaaatgtaca tctagattta
     1561 tagccatcta aaactcttgc tttttttgta gaagatacga aaaaagccat gaaaccaagg
     1621 cttttcctgt tttatgtaga tgtttacaca tgaaaattga agtattttca aaagctaatt
     1681 ggagtgattt tattcatgat gggataaatg atctggccaa aaatgattga gaaatagttg
     1741 cttaaaaagc atttgcatga tactatatat tgaaaggagg tgataatatg gtagaacaaa
     1801 gaaaatcaat taccatgaaa gatgttgctt tagaagcagg agttagtgtt ggaactgttt
     1861 cacgtgtaat taataaagaa aaaggcatta aagaagtaac tttgaaaaaa gtggaacaag
     1921 cgattaaaac tttgaattac attccagatt actacgctag aggaatgaaa aaaaatcgaa
     1981 cagaaacgat tgcaatcatt gtaccaagta tctggcatcc cttcttttca gaatttgcta
     2041 tgcatgtgga aaatgaagtc tataagagaa ataacaaatt actcttatgt tctatcaatg
     2101 gtacaaatag agagcaagac tatctggaga tgttgcgtca taataaagtt gatggagtgg
     2161 ttgccattac ctatagtcca attgaacatt acttgacgtc aggaattccc tttgttagta
     2221 ttgaccgcac atactcagat attgccattc cttgtgtttc atcagataat gatgcaggtg
     2281 gaagagaagc agctaaacaa ttaattagta aaggatgtca gcatttagcc tttgtgggcg
     2341 gacataatac aaccattaat gaaaccaaac gtcgtagaat ttcatttgaa aagtatgtcc
     2401 aagagcataa aatacctctt agtatttttg atttggatga gacagttact gactattatg
     2461 gaaagctaga gagtttcttg gaagaaaatt cttctataga tggaattttt acaatcaacg
     2521 attttacagc tttggatgtg attgaaatac taaaaaaaaa tggcaaacgt attcctcatg
     2581 atgttcaaat tattggatac gatggaatta aaatggctgg agatagagat tatctacttt
     2641 caactattga acaaccattg gaagaaatgg caaaagaagc ggttcgtatt ttgtttgata
     2701 taattgatgg aaagactgtt aatttgcaga caatcctacc agtaaaattt gttgaaggaa
     2761 aaacaacaaa aaatgaaaat aagtcttgac agaaagcgct atcaatgata gaatgaattc
     2821 agataaaaag atttattttt aaaacaaaaa tgaaacgttt caaaaaaaga aataaagaga
     2881 cagcgtcaag cgctatcttt tctagaaaaa atgaaacgtt tcaaaaagga ggttgctatg
     2941 aatagcaaag cgaagcaagt ttctctttgg gaaagaatca agaaacaaaa actcttgtta
     3001 ttgatgactg tccccggttt agttttaacc tttatcttta aatacatccc tatgtatggg
     3061 gttttaatcg catttaaaga ttacaatcct ttaaaaggaa ttttagggag tgattggatt
     3121 ggtttttctg agtttacaaa attcatatcc tctcccaact ttggtatctt gttagccaac
     3181 acattaaaat taagtatcta tggtttattg cttggctttt taccaccaat cattcttgct
     3241 attatgctca atcaactctt gagtgaaaaa gtcaaaaaac gaattcagct cattttatac
     3301 gcaccaaact ttatctcagt cgttgttatt gtcggtatga ttttcctctt cttttcagtg
     3361 ggaggaccaa tcaacaattt tctttctatg tttggaatga aggctgactt cttgacaaat
     3421 ccagacttct ttagaccttt atacatcttt agtggtatct ggcaaggaat gggctgggct
     3481 tcaacgctct acacggcaac attggtaaat gtagatccag ccttagtaga agcagctcga
     3541 ctggatggag ccaatatctt ccaacgaatc tggcacattg atattccagc tcttaagcct
     3601 attatggtta tccaatttgt tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa
     3661 gcattcttga tgcagacatc gttaaatttg ccaacttctg aaattatctc gacatatgtc
     3721 tataaagttg gtcttgtatc aggagactat tcttactcaa cagcggttgg tttgtttaat
     3781 gcagtgatta acgtagtatt gcttgttgca gttaaccaaa tcgttaaacg catgaataat
     3841 ggtgaaggaa tttaaggagg aaagtatgaa aaattcgatt atggatacaa aatttgatag
     3901 acgtatctta ctcttaaata aaatcattat tgtctttatc gttttgatga ctttgcttcc
     3961 tttactttat atcgtcgtag catcctttat ggatcctaag gttctggtta gtagagggat
     4021 tagctttaat ccagccgatt ggactgtaga aggttaccag cgtgtattca gtgaccaatc
     4081 tattctaaga ggttttatca attctctact atactctttt ggatttgcag ctttaacagt
     4141 cttgctatct gtgtttacag cttatcctct ttctaagaaa gacttggttg gacgtcgttg
     4201 gattaactac ttcttgattg taactatgtt ctttggtggt ggtttagtcc caacttactt
     4261 gctcgtaaaa gaattgggaa tgctcaatac tccatgggct atcattgttc caggtgctgt
     4321 caatgtttgg aatattattc ttgctagggc ctatttccaa ggattgcctg aagaattagt
     4381 tgaagctgct gtcattgatg gtgcaaatga tttacagatt ttctttaaaa tcatgcttcc
     4441 tcttgcaaaa ccaattatgt ttgttctctt cctctatgct tttgtaggac agtggaactc
     4501 atactttgat gcaatgattt atatcaagga tccaaacttg gaaccattgc aacttgtact
     4561 tcgtaaaatt ctcattcaga gccaaccagg tcaagacatg attggagcac aagcggctat
     4621 gaatgaaatg aaacgtttag ctgaattgat taaatacgca actattgtca tttccagctt
     4681 gccattgatt gttatgtatc cattcttcca aaaatacttt gataaaggaa ttatggctgg
     4741 ttcacttaaa ggataaaaaa agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat
     4801 tctcaaaatc agcagttttg ttgacagcta gtttagcagt acttgcagcc tgtggctcaa
     4861 aaaatacagc ttcaagtcca gattataagt tggaaggtgt aacattcccg cttcaagaaa
     4921 agaaaacatt gaagtttatg acagccagtt caccgttatc tcctaaagac ccaaatgaaa
     4981 agttaatttt gcaacgtttg gagaaggaaa ctggcgttca tattgactgg accaactacc
     5041 aatccgactt tgcagaaaaa cgtaacttgg atatttctag tggtgattta tcagatgcta
     5101 tccacaacga cggagcttca gatgtggact tgatgaactg ggctaaaaaa ggtgttatta
     5161 ttccagttga agatttgatt gataaataca tgccaaatct taagaaaatt ttggatgaga
     5221 aaccagagta caaggccttg atgacagcac ctgatgggca catttactca ttcccatgga
     5281 ttgaagagct tggagatggt aaagagtcta ttcacagcgt caacgatatg gcttggatta
     5341 acaaagattg gcttaagaaa cttggtcttg aaatgccaaa aactactgat gatttgatta
     5401 aagtcctaga agctttcaaa aacggggatc caaatggaaa tggagaggct gatgaaattc
     5461 cattttcatt tattagtggt aacggaaacg aagattttaa attcctattt gctgcatttg
     5521 gtatagggga taacgatgat catttagtag taggaaatga tggcaaagtt gacttcacag
     5581 cagataacga taactataaa gaaggtgtca aatttatccg tcaattgcaa gaaaaaggcc
     5641 tgattgataa agaagctttc gaacatgatt ggaatagtta cattgctaaa ggtcatgatc
     5701 agaaatttgg tgtttacttt acatgggata agaataatgt tactggaagt aacgaaagtt
     5761 atgatgtttt accagtactt gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg
     5821 gtatgggatt tgcacgtgac aagatggtta ttaccagtgt aaacaaaaac ctagaattga
     5881 cagctaaatg gattgatgca caatacgctc cactccaatc tgtgcaaaat aactggggaa
     5941 cttacggaga tgacaaacaa caaaacatct ttgaattgga tcaagcgtca aatagtctaa
     6001 aacacttacc actaaacgga actgcaccag cagaacttcg tcaaaagact gaagtaggag
     6061 gaccactagc tatcctagat tcatactatg gtaaagtaac aaccatgcct gatgatgcca
     6121 aatggcgttt ggatcttatc aaagaatatt atgttcctta catgagcaat gacaataact
     6181 atccaagagt ttttatgaca caggaagatt tggacaaaat tgcccatatc gaagcagata
     6241 tgaatgacta tatctaccgt aaacgtgctg aatggattgt aaatggcaat attgatactg
     6301 agtgggatga ttacaagaaa gaacttgaaa aatacggact ttctgattac ctcgctatta
     6361 aacaaaaata ctacgaccaa taccaagcaa acaaaaacta gaggttgatt atgggagata
     6421 agaaatacac agtagaaaaa gccaatcgtt ttatagcaga aaataaacat ctcgttaata
     6481 ctcaatataa gcctgaagaa catttttcag ctgagattgg ttggatcaat gatccaaatg
     6541 gatttgtcta ttttcgtgga gaataccatc tcttttatca attctatcca tatgatagtg
     6601 tttgggggcc tatgcactgg ggacatgcta aaagtaagga cttggtgact tgggagcact
     6661 tgccagtggc acttgctcct gaccaagatt atgaccgaaa tggttgtttc tcaggctctg
     6721 ccattgtcaa ggatgatcgc ctctggctca tgtacactgg acatatcgaa gaagaaaccg
     6781 gtgtccgcca agtgcaaaat atggcatttt cagatgacgg gattcacttt gaaaagattt
     6841 cccaaaatcc agttgcaact ggatcagact tgccagatga gttgattgct gctgatttcc
     6901 gtgatccaaa actctttgaa aaagatggac gctattactc cgtagtagct gccaaacaca
     6961 aggataatgt gggctgtatc gttctactag ggtccgataa cctagtagaa tggcagttcg
     7021 aatccatctt tttaaaaggg gtagaacacc aaggctttat gtgggaatgc ccagattact
     7081 tcgagttaga tgggaaagat tgccttatta tgtcacccat gcgttatcag cgtgagggag
     7141 actcatatca taacatcaac tcatcgcttt tgttcacggg taaggtagat tggggagaaa
     7201 aatgttttat cccagaatca gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc
     7261 aaacattgtt ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag acatgggggc
     7321 gtacccttcc aacccatgac caagaacaca aatgggcatg tgccatgact ttacctagaa
     7381 ttctaagatt ggaagatggc aaactaagac aattccctgt taaaaaaggc caatatcaaa
     7441 tccaaataga taaagattgt cattaccact taggaaatga tatagattat cttgaatttg
     7501 gttatgatag taatgcgcag caagtttaca ttgatcgtag ccatcttatt caaaaaattc
     7561 taggtgaaga agaacaggac actagtcgac ggtatgtaga tattgaagct aaagaattgg
     7621 aagttgttct agataaaaat tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga
     7681 ctgcaactta ttacttaacg gtgccagctg agctatcacg aattgattaa aaattaagtt
     7741 atttctccta aagaaaaagt tctctttcta aaatagtgga aagaggactt tttgtgtttt
     7801 gggtatataa gcttagttta tggtatttgt aaaattggtg ttggattatg atttaagcta
     7861 gttttctaaa gaatttgaaa aaaattttat ttaagcaaaa aaaccttggt tccaaggctt
     7921 ttcctgttgt atttagatgc cccctacagg gatcgaacct gtgacccacg gattaagagt
     7981 ccgctgctct gccagctgag ctaaggaggc aaagaaaaaa gctgtattgg tgccgaaact
     8041 tcacggtttg tattgaaccc gcgcaattaa gcaggtgggc aactcgctct aactgaagct
     8101 gtttccgtgt gagacggcct acatgctgtt agaagacttt tgtttcccta ataatacaaa
     8161 aaatagtcgg tcaacactta agtgtgaagt cgtacaccac agcgtttcta tatttatatg
     8221 ataccacttt ttcaaaaaaa atcaagagga aagtgcagtt ttttgaaaag gatttcagat
     8281 ttttcgcaaa tggtcgatag cttcctttct ttgagccaaa gcccgttcat atttaccagt
     8341 atcttctgct tggaaatagt gatgattacg aattttttct ggcagatagt cttgcttgac
     8401 ccaatttcca ggatagttgt gtggatagag atagtcttgg gcattcccca gttccttgct
     8461 tccactgtag tgcccatcac gcaggtgtcg cggaataggc aagtgccctg atgttttgag
     8521 gtcagcaagt gccttatcca tagctacata ggctgagttg gattttggag aaagggccaa
     8581 atcaatcacg acattggcaa tgagaatgcg ggcttctggg aacccaatct tctgggcagc
     8641 atccagagca gtcacggtat gaatctgggc ctcaggattg gctaagccga tatcttcata
     8701 ggcgataaca gtcaagcgac gagcgagact aggcagatcc ccagcctcaa tcaagcgggc
     8761 agtatagtgg agactggcat ccacatctga gccacgaata gacttttgca gggcagatag
     8821 aacatcatag tgaccgtctc catccttgtc catagtgatg tagcttctct gaagactatt
     8881 ttccatgatg tctaaggtga tgtggcgaat gccctcgtca ttctcagggg tagagagaac
     8941 agctaagtcc agtgagttaa aggcagatcg aaggtctccg tttgtagagg ttgcgatgaa
     9001 atccagcgca tcctcatcta gttctattgg aaaatcaaaa ccacgttcag ggttacttag
     9061 agctatctgc agggcctctt tgacgtcttg gttagacaga ggttccaact cgaaaatttg
     9121 aactcggcta cgaatggcag gagtgacaga gaagaaagga ttttcagtcg ttgctccaat
     9181 catgatgacc agtccacttt ccaagagagg caagaggaag tcttgcttgg tcttatctag
     9241 tcgatgaatt tcgtctagca ataggacgag accaccagaa aatttagctt cttccgagat
     9301 ttcttgcagt cgctttttac tatcaactgt cgcattgaaa gttcgaaagg catacttggt
     9361 cgttccagcg atggcagagg caatactggt tttgccgatt ccaggagggc catatagaat
     9421 catggaggac aggcggttgg cttccaccat gcggcggatg atttttccag gtccgaccag
     9481 atgctcctga ccgatgacct ggtcgatggt tttagggcgc atgcgaagcg cgagattgtc
     9541 tggcatagca gtcctttcta acgtagattt gagtagaatg actcatctac tatatcttac
     9601 tatgtccatt ctagcctatt agttgtgata tttcaatggt tttgtaataa tcagggagtg
     9661 agatagaatc ttttttacta tttaatttaa tgaggagagg tatcagcatg agagatttga
     9721 ttttccttaa agctcaaaat aggatagttg agcctaaaga agtggaagtg ctgacttgag
     9781 aaataatatt gctaaatgtc attgtatgaa ctataatgaa aagagaggtg ataagcaatg
     9841 gaaatgatgg aactaccaag tcaggaaatt ttgattttta caaaacaaat tcgtcattgg
     9901 attcttagtg atcaagttat ttcagggaaa agaaaacttt tcttctggga agatactcca
     9961 aaagagattt tagatttgta cgaaaacatt aaatctaaac ttgattttgc ttaccaagaa
    10021 gttcattcta ataatgggtt gaaaaaatat gaaaaataga ataattgatg tttttgaagt
    10081 agtaaatcgc ctccttgtaa taactgttga aaatcctgat ttcgaagact tgagagttaa
    10141 tcatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg tacttgagta
    10201 cggtaaggcg acgttgacgt ggtttgaatt tgattttcga agagtatcag tttgtaaaaa
    10261 ttggagataa aaaatataga gtacgcagtg gtcctatgat tcattcaact ccgcctcaat
    10321 ctgtcttaga tcgagatact tttacaattg actatacaga tgatgaattg ctcgataaag
    10381 aagcagtttt tactactcat tagaaaaata ggtatgaata aattggcttc ccttagaacc
    10441 acatctaggg gcttttatgt ataatagaat aataagtatc actcctttac aatacgtgta
    10501 acacgtgtta taatggtgtt gtcaggaggt aaaagcgcta tgcctatgac acaaaaagag
    10561 atggttaaac tccttacggc ccatggttgg ataaagacta gaggcggtaa aggctcccat
    10621 attaaaatgg aaaagcaagg ggaaagacct atcacaatcc ttcatggtaa gctgaataag
    10681 tacactgaaa gagggataag aaagcaagct gggttgtaaa attcagcccc tgctttttga
    10741 tatagaggta gtgaaatgtt agttacgtat ccagctttat tttattatga cgacacagac
    10801 ggaacagaag cgacttattt tgtccatttc ccagattttg aatactcagc tacacaagga
    10861 gaggggattt ctgaggcttt ggctatgggg tcggagtggc tagggataac tgttgcagat
    10921 ttgatagaga gtgatggaga gttacctcag ccatcagata ttaatagctt gtctttaatt
    10981 gataatgatc cttttaaaga tgatgaagat ttcgtgtcaa cctatgacct tgataaatct
    11041 tttatttcta tggtatctgt tgatgtatca gaatacctag gaagtcagga acccattaaa
    11101 aagactttga ctataccaaa atgggcagat aagttgggac gagaaatggg acttaacttt
    11161 tctcagactt tgacagacgc tattgcagat aagaaagttc aagcctaaga ttaagcgtgg
    11221 tatcgtggta atagtcaata aaaaagcacg tccacttgtg ctagttactc gcctattgat
    11281 tttatagatt tattagccct ttcgcgggct ttttatattg atttttatga gaaatgaaga
    11341 aatcagactt ttaagaaaat cattgatacc aagggtttaa atgaggtaat atggtataat
    11401 taggacataa aataattttg tggtaagatg gtagtatcta ttttagcata tttccgagca
    11461 atcggggcga ttaaagagtc gcatagaaag aggacaaaat ggcaacatac ggatttttag
    11521 atattttaga ggaagagttg gacaagaact ttccctttga ctttgagatt agttgggaca
    11581 agcgtaacca tgcggtagaa gtgagttttc tattggaagc gcaaaatgct gcaggtgtgg
    11641 agatggtgga tgaagacgga gaggttttat cagatgacat tctttttgag gaagcggtcc
    11701 ttttctacaa tcctgccaag tcaacagtca atgaggaaga ctatttgact gttattcctt
    11761 acctgcctaa aaaaggattt tcccgtgaat ttttagctta ttttgcgctt ttccttaaag
    11821 atactgctga ggttgggcta gatgtcctca tggacttttt ggaagaccca gaagcagagg
    11881 aatttgtcat ggaatggaac caagaagtct ttgaagaagg aaaagttggc ttggaagagg
    11941 gagaatttta tccttatcca agatactagg agttggttgg aggtgttatg aagaaaattg
    12001 ggacttattt ggtttatgtg ctagctgttg tctttattat gctagctttt gcttgtggaa
    12061 caatcgcatt tgcagagttg gggtattccg cagttttagc ctttactttt ggttatgcct
    12121 tcgctcttct aagcatgtat ttaattttta ttcttcatga gctgggtcat gctttttgtg
    12181 gctacttgac aggctatcgg ctggtggctt ttggattagg acattttatt ttgaccaaaa
    12241 agtcaggcag gtttcatctt agtagaacag ccattctgaa aaatgttggt gctcaataca
    12301 ttggtttaaa agaggatgaa agcgatcaaa gaatcatcct gatgctttca ggaggcttga
    12361 tggttcatct cggcttgata ttattggcga tagtgtttgg atttttgaca agaagctggt
    12421 attttgcagg gacttggatt tttcttaatt tatctttctt cctaaataac attttgccag
    12481 tcgacatcac cgatggagca aaaatttggg aattactaca acaccctgaa aataccaaat
    12541 acgcctacct ggtattgaga cattctgctc aaactttgct ggctcctcaa gaatatgatt
    12601 tgaaagactt tgttcaagca gttcctgagg atgcacgagg gagttttgcg gatggtgtat
    12661 taggtatgca aggtgaagtt tccattttgg aagggaagga agaagtagct aaacagcagt
    12721 ttcaagcatt acttgagagg gcaactactc cgatgatgca gacagttgcc caactatccc
    12781 tgcttcatat cgccttgttg gaaggagatt ttgagcaagc ggagcaatat gctagtattc
    12841 gacaggtcaa atcgtttctg tctctcaaac tgtccaatct tcaagtgata caagcctggt
    12901 atcaattaaa ggtgaagaaa gatgtagatc gaactcgtaa ggctctcaag attgctagac
    12961 agaaaatgga tagtagtcga atgttgaggg acgagaaacg ctattatgaa aacttgttag
    13021 ccgagctgga aaaggaaatg ttagaaggaa ctgaagatgg aactagaaat ttctggtttc
    13081 ccaggctgta agattgcttt gttttgtgag gataagctct tgactatctt acgtgatgat
    13141 aaggcaagta ttccctgggc caatatgtgg gaactgccag gtggtggacg tgaaggtgat
    13201 gaaagtcctt ttgagtgtgt agcgcgtgaa gtttatgaag aactgggcat tcatctgact
    13261 gaagattgtc tgctttggag taaggtttac ccaagtatgc tttttgcaga caaacagtcc
    13321 gtttttctag ttggtcagtt gacacagaac cagtttgaca gtatcgtatt tggagatgaa
    13381 ggacagggct atcagctgat gaatgttgag gaatttctta gttccagtca agttgtccct
    13441 cagttgcaag agagattaaa agattattta aaagtaagtg attagaaagg atggttcagt
    13501 taaatttcta aactgaaccc gccctaaaca ctgtgccaaa aagataaact tctcttagac
    13561 acaagcgtct tcagagaatt tcctattttg gttttgtgtt ttacgggctt ggtatcttaa
    13621 taatggaaac atggcaagag ttaaaagtta cagtgaagcg tgagggagag gagttggttt
    13681 ccaatctctt gattgagctg ggagcgcaag gagtcgcaat cgaagacagt atggactatg
    13741 tgggaaatgt tgaccgcttt ggcgagattt tcccagaggt ggagcagcaa gaagaaatcg
    13801 ttgtgacagc ctactaccct gacacggtgg atgtgacagt ggttgaggcg gacttgcagg
    13861 ctcgcatatc agaattgacg gattttatgg atttgggaga gttaaaaata ggtacgactg
    13921 ccttggctga ggaagactgg gcagacaact ggaagaaata ctatgagcca gctcgtatca
    13981 ctcatgattt gaccatcgtt ccctcttgga cagactatga ggcgactgcg ggagaaaaaa
    14041 ttatcaagct ggatcctggc atggcttttg gtactggaac ccacccaact accaagatga
    14101 gcctttttgc cttggaacag gttcttcgtg gtggcgaaac ggtgctagat gtggggactg
    14161 gttcaggggt tctctctatt gccagctcgc ttctgggggc taaagaaatt ttcgcctatg
    14221 acctggatga tgtggcggtt cgtgtggctc aggaaaatat tgagctcaac cctggcatgg
    14281 aaaatatcca tgtagcggct ggagatttgc ttaagggtgt ggaaattgag gcagatgtga
    14341 ttgtggctaa tatcttggcg gatatcctca ttcatctaat agacgatgcc tatcgtttgg
    14401 ttaaggacga aggctacctg atcatgagtg gcattatcaa ggacaagtgg gacatggtgc
    14461 gccagtcggc tgagtcagct ggatttttcc tcgaaactca catggttcaa ggggaatgga
    14521 atacctgtgt ctttaagaaa accaaggata tctctggtgt gattggaggc tagcatgcag
    14581 cagtattttg taaaaggcag tgctatctct cctgtcacta tcgaggacaa ggaaaccagt
    14641 aagcatatgt ttcaggttat gcgcttgaaa gaagaggatg aggttacctt agtctttgat
    14701 gatggcatca agcgcttggc gcgcgtgctg gatatggaaa atcgtcagtt tgagttggtc
    14761 caagaattaa atgacaatgt ggaactacca gtccaagtga ccatcgcatc tggatttccc
    14821 aagggagaca agctggagtt catcgctcaa aaagtaaccg aactgggtgc tagccaaatc
    14881 tgggcctttc ctgcagactg gtcagttgcc aagtgggatg gcaagaaatt gggtaaaaaa
    14941 gttgaaaaac tagaaaaaat tgcccttgga gcagccgagc aaagcaagcg taatattgta
    15001 ccaagtatta aacttttcga gaaaaaagca gattttctag ctcagctgga ccagtttgac
    15061 tttatcatag tagcctatga agaatcagct aaagaaggag aagccgctgc gcttctgcaa
    15121 gcagtcactg gtcttgaaac aggagccaaa ctgctcttta tctttggtcc agaaggcggt
    15181 ctctcacctg cagaaatcga aagttttgaa gctaagggag ccgttttggt aggcctaggt
    15241 cctcgtattt tgcgagcaga aacagcaccg ctttacgcct tatcagccct tagtgtttta
    15301 gtagaattag agaaataaga ggaagaaaat ggaacaaaaa caccgttcag aatttccaga
    15361 gaaggaactc tgggacttaa cagccctata ccaagaccgt gaggatttct tgcgtgcaat
    15421 cgagaaagct cgtgaagaca tcaaccagtt tagccgtgat tacaagggca atcttcacac
    15481 ttttgaggat ttcgagaagg cctttgcgga attagaacag atctacattc agatgagcca
    15541 tattggcaac tatgctttta tgcctcagac gacggactat agcaatgacg aatttgccaa
    15601 tattgcccaa gctgggatgg aatttgaaac agatgccagc gtagccttga ccttctttga
    15661 cgatgccttg gtggtagcag acgaggaagt cttggaccgt ttgggtgaat tgcctcactt
    15721 gacggcagct attcgtcagg ccaaaatcaa aaaagcccac tatctagggg ctgatgtgga
    15781 gaaggccttg accaatctcg gtgaagtttt ctatagtccg caggacattt atactaagat
    15841 gcgagctggg gattttgaaa tggctgactt tgaagcccat ggcaagacct ataaaaacag
    15901 ctttgtcacc tatgagaatt tctaccaaaa tcacgaggat gctgaggttc gtgagaaatc
    15961 cttccgttcc ttctcagagg gacttcgtaa gcaccaaaat acggctgcag cagcctatct
    16021 ggctcaggtc aagtctgaaa aactcttggc tgatatgaag ggatacgact ctgtatttga
    16081 ttatcttcta gctgaacaag aagtggaccg tgtcatgttt gaccgccaga ttgacctcat
    16141 catgaaggac tttgcaccag tcgctcagag atacctcaag catgttgcca aggtaaatgg
    16201 tcttgaaaag atgacctttg cagactggaa attggacttg gacagcgccc tgaatcctga
    16261 agtgactatt gacgatgcct atgatttggt catgaagtcg gtagaacctt tggggcaaga
    16321 atattgtcag gaagttgctc gttaccaaga agagcgctgg gtggactttg ctgctaacag
    16381 tggcaaggat tccggtggtt atgcggcgga cccatatcgc gtacaccctt atgtactcat
    16441 gagctggaca ggccgtttga gcgatgtcta taccttgatt catgaaatcg ggcattctgg
    16501 tcaattcatc ttttcagaca atcaccaaag ctatttcaac gcccacatgt cgacctatta
    16561 tgttgaagca ccgtcaacct tcaatgaatt gctactcagt gattacttag agaaccagtc
    16621 taatgaccca cgtcaaaaac gcttcgctct ggctcatcgc ttgacagaca cctacttcca
    16681 taatttcatc acccacctct tggaagccgc cttccagcgt aaggtgtata cattgattga
    16741 agaaggggag acctttggag caagcaagct caacagcatt atgaaggaag ttttgacaga
    16801 tttttggggc gatgccattg aaatcgacga cgatgcagct ttgacttgga tgcgccaagc
    16861 tcactactat atgggcttgt atagttacac ttactctgct ggtcttgtca tctcgacagc
    16921 tggttacctt catcttaaaa actcagaaac tggagctgaa gactggctca atctcctcaa
    16981 atcaggtggt agcaagacac cacttgagtc agccatgatt atcggtgctg atatttcaac
    17041 agacaaacca ctccgtgata ccatccaatt cttgtctgac acagttgacc agattatcgc
    17101 ctacagtgct gagttggggg agtaagggga atagaaaaat tttttagaat gatgtttatg
    17161 ggcttgttaa gcgtcattct atttgtctta gttgtagcta ttttcaaaaa tgtcgttgaa
    17221 ggtcgtgatg ttaaatttga acttactgta gaactggtat tgatagttat ttacgtcatt
    17281 ttatatcaga ttgtatttaa ttgagaattc aatattgagt catttaagac tgcctttttg
    17341 aacagttgct agaaatgtag aggtattgac catgtatcaa gatttactaa gaaaaatagc
    17401 agaagaaaaa ccaaattata accaggagga aatccagtgg ttgcttgatc atttgggaga
    17461 tccttctcca gaaattcgcg atgaccttgt ttttacaagc tttgctagag ggattcagga
    17521 agagctattt acacaggagc aatttcattt cattgctgaa ggggtttcat ctgatgtagg
    17581 actagataaa gagattgata aggtaggtct gccaacactt gaacgttctt ttagggcact
    17641 tgtttatgca aatctcttgt atgcagatgc caaccagcaa tcggtttttt atcagggatt
    17701 acaattagaa attcgtaatg tcttttcaaa tcaaggcttg cactatcttt caaaagaaaa
    17761 ggatacaaca ggtttctcaa gtcagtatgg ttgggttcat gcttttgcac atggagccga
    17821 tttactgaca gaggtggtct gtcatccaga ctttcctata aacagaattc atgaagtatt
    17881 tgacatactt ggccaactat ttaaaagaat gtcgattcgc tttacagatg atgaggattg
    17941 gcgtttagca agagtgatct atgaacctat tttacaaggg aagttggagc aagaacaagt
    18001 agcttcttgg ataaaaactg ttgattttcc gatagaagaa agggaggatt tttccaaatt
    18061 ttccaacttt agatcctgtc tggtggaagt ctatgtccaa cttgaccaga gaaatagttt
    18121 acaagatgag ttgaaagaag ctatccagtc ttttcaatac tagagattag ctaatagttc
    18181 tatgattttc aaaaaatttc caatcaattt tcttgaaacc ctttcctact tttgatagac
    18241 tagtatctag tttttgaaaa aaggagaaag aaaatgaaaa aatttgttgc tgagttaatc
    18301 ggtacgttca tgcttgtgtt cgttgggaca ggagctgttg tttttggaaa tggttttgat
    18361 ggccttggtc accttggaat cgcctttgcc tttggtttgg caatcgtggt ggcagcctac
    18421 tcaatcggaa ctgtttcagg tgctcacttg aacccggctg tttcgattgc tatgtttgta
    18481 aacaaacgtt tgtcatcttc agaacttgta aactacatcc ttggtcaggt tgttggagct
    18541 ttcatcgctt ctggcgctgt cttcttcctc ttggctaact caggtatgtc aactgctagt
    18601 cttggtgaaa atgccttggc aaacggtgtc actgtctttg gtggtttctt gtttgaagtc
    18661 atcgcaactt tcttgtttgt attggttatc atgactgtga cttcagaaag caagggcaat
    18721 ggcccgattg ctggtttggt aatcggtttg tcattgatgg cgatgattct tgtcggattg
    18781 aagattactg gactttcagt aaacccagct cgtagcttgg caccagctgt cttggtaggc
    18841 ggcgcagccc ttcaacaagt ttggattttc atccttgcac caatcgctgg tggagttctt
    18901 gcagcccttg ttgcaaaaaa tttccttgga acagaagaat aattgaaact caaaaagcct
    18961 tgctcctcat cttgaggaac agggcttttt cgtatgatac tcttcgaaaa tctcttcaaa
    19021 ccacgtcagc ttcaccttgt cgtaggtatg gttactgact tcgtcagttc tatctgcaac
    19081 ctcaaaacag tgttttaagc tgacttcgtc agttctatct gcaacctcaa aacagtgttt
    19141 tgagtaacct gcggctaact tcctagtttg ctctttgatt ttcattgagt atgactttag
    19201 cggttgtcaa ttttctctgg ataaaggtcg tgttggaaga ggcgttgttc tgccaagccc
    19261 tcatacttag ttccttgctt accgtagttg tagtaggggt tgattgaaat gccaccgcgc
    19321 ggagtgaatt tcccccagac ttctaaatag cgagggtcta gcaagttgac caagtctttc
    19381 ccgatggtgt tgatacagtt ttcgtggaaa tctccgtggt ttcggtagct aaatagatat
    19441 agtttgaggg attttgactc gacacagagt ttgtcaggaa tgtaggaaat atgaatcgtc
    19501 gcaaagtctg gctgagcagt gatttgtccc agcagagaca tatcgaggat atggtgacga
    19561 atgccctgtt ccttagcgat ttctctatta atttgaattt cgaggtgatg acgttggccg
    19621 taggcaaagg tgacagcttc gactgtttca tagtactcct tccaaaatgg gaaatgttca
    19681 gagcatgcaa aaagctccca ttagggagct aaaaaatacc aaatcgagtt tttttagcga
    19741 tggcatatcc caaacatcgt aatattctac ttatatagta aaa
//
LOCUS       DAVLTM010000024        19570 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLTM010000024 DAVLTM010000000
VERSION     DAVLTM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.024"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(151..1119)
                     /locus_tag="VYN16_000785"
     CDS             complement(151..1119)
                     /locus_tag="VYN16_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1403343.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLNLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            1255..2112
                     /locus_tag="VYN16_000786"
     CDS             1255..2112
                     /locus_tag="VYN16_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1403344.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            2099..2878
                     /locus_tag="VYN16_000787"
     CDS             2099..2878
                     /locus_tag="VYN16_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1403345.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            2902..4254
                     /locus_tag="VYN16_000788"
     CDS             2902..4254
                     /locus_tag="VYN16_000788"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1403346.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            4328..4702
                     /locus_tag="VYN16_000789"
     CDS             4328..4702
                     /locus_tag="VYN16_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1403347.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            4704..5552
                     /locus_tag="VYN16_000790"
     CDS             4704..5552
                     /locus_tag="VYN16_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1403348.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            5829..6596
                     /locus_tag="VYN16_000791"
     CDS             5829..6596
                     /locus_tag="VYN16_000791"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1403349.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            6608..7792
                     /locus_tag="VYN16_000792"
     CDS             6608..7792
                     /locus_tag="VYN16_000792"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1403350.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSLLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            7789..9660
                     /locus_tag="VYN16_000793"
     CDS             7789..9660
                     /locus_tag="VYN16_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1403351.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(9803..10987)
                     /gene="mntE"
                     /locus_tag="VYN16_000794"
     CDS             complement(9803..10987)
                     /gene="mntE"
                     /locus_tag="VYN16_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1403352.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            11291..13987
                     /locus_tag="VYN16_000795"
     CDS             11291..13987
                     /locus_tag="VYN16_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1403353.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            14000..14791
                     /gene="yghU"
                     /locus_tag="VYN16_000796"
     CDS             14000..14791
                     /gene="yghU"
                     /locus_tag="VYN16_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1403354.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            14907..15317
                     /locus_tag="VYN16_000797"
     CDS             14907..15317
                     /locus_tag="VYN16_000797"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1403355.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            15329..16924
                     /locus_tag="VYN16_000798"
     CDS             15329..16924
                     /locus_tag="VYN16_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403356.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            16990..17154
                     /locus_tag="VYN16_000799"
     CDS             16990..17154
                     /locus_tag="VYN16_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1403357.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            17233..17961
                     /gene="yaaA"
                     /locus_tag="VYN16_000800"
     CDS             17233..17961
                     /gene="yaaA"
                     /locus_tag="VYN16_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1403358.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(18012..19496)
                     /locus_tag="VYN16_000801"
     CDS             complement(18012..19496)
                     /locus_tag="VYN16_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1403359.1"
                     /translation="MRNLLSTKVQRQLRLMENLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIINLVNHLNTLSNEIHLSNTL"
BASE COUNT         5721 a         3672 c         4474 g         5703 t
ORIGIN      
        1 ctctgtaaaa tgatgtaaaa acatttattt aaaagctcaa aatatagctg ttttaaaagg
       61 tattgtaaga tatagtaaaa cgatgtaaag gtatttttaa atgcggatga attataaagc
      121 cacgaatcct atgtgactag tggttctttt ttataaactg gtagagtgtt ttggttgtac
      181 tttttgttca gggtcaatgt agttgatagc gttgttgaca gcagttggag cttctccgag
      241 gcctgtcgca atcagatcaa tttttccgtc atagtagcag cagtcaccga tagcatagat
      301 acctgcttgg ctggattcct gtttgctgtt gacgataatc ttgtgacggt tgaggttgag
      361 cccccagttt ttaaggttac cgacagaaga tttgaaacca tagttgacaa agaggtggtc
      421 taggtcaatg gtttcagttt catcagattt gacttttgtg atttcaagtt tatcaagtgt
      481 ttttccattt ccaaggagtt ggctaggggc gaatggtgtc ttgatggtta cagatgattc
      541 ttgcaaggct tgaacactgt gttccaaggc acggaaatta tctctgcggt gaacaagggt
      601 agttggtgcg attttttcaa aagccaaagc ccaatccaca gccgagtctc ccccaccaag
      661 aatcgtcact ttcttaccag cgtattgctg aatgttagaa acgtggtagt ggatattttc
      721 atagccctca acaccttcaa gttccagcgg acgtggtttg aaggcaccgc cacccatagc
      781 gataatgact gttttagtca ggtgacttcc tttagaagtt gtgatggcaa attcttcttc
      841 ttgtttgtca atctcaagaa ccgtttcatt gagatgaata ggggtatcaa atccatttag
      901 ctgttcaatc aagcggttag tcaactcttc tccagtcagg tttgggaagc ctggtacgtc
      961 taggatttcc ttttcagggt agagaatagc aggttgtcca cctagctggg gaagagagtc
     1021 gatgatttga accttggctt ggcgtaggtg ggcataaaag gctgcaaaaa gcccgacagg
     1081 accaccaccc acaatggtaa tatcatagag ttgagacata gtttctcctt tgttttttct
     1141 agtcagttta ttttatcata tttttgttca atgtaaaatg gactaggata gggaaaaaaa
     1201 tgccagaaaa tggtataata ggacgaaagt gttttgatag ggaggatatc agggatgaat
     1261 tttcaacaat tatccaatct gcaatattgg accagtttgt ttgcaagtcc atggacgata
     1321 gctatcaatc tgattgatat tttgattgtt gcttatattt tataccattt tacaaaagct
     1381 attgcaggaa ccaagattat gattttggta cgtggagttt tggtgtttat tttagctcaa
     1441 atccttgcaa atatgattgg tttgactacg atttcttggt taatcaatca aattattact
     1501 tatggggtta ttgcggcggt tgttatcttc tctccagaga ttcggactgg tttggaacgt
     1561 ttgggaagag cgacagattt cttttccaat gcccctatta gtgctgagga acagatgatt
     1621 cgtgcctttg ttaagtctgt tgaatacatg agtcctcgta aaatcggggc cttggttgct
     1681 attcagcgtg tacgtacctt gcaggagtat atttcgacag gaattccctt ggatgctaag
     1741 atttctgcag aacttctcat taacattttt attcccaaca ctcccctaca tgatggtgcg
     1801 gtgattatca aagaagaacg tatcgctgtg acgtctgcct atctgccctt gacaaaaaac
     1861 acagggattt ccaaggaatt tgggaccaga caccgggcgg ctatcggttt atcagaagtc
     1921 tcagatgcct tgacttttgt cgtatcagag gaaacgggag gaatttcgat aacctataat
     1981 ggaaggttta agcacaacct aacacttgat gaatttgaaa cagaattacg tgaaatctta
     2041 cttccaaaag aggaagtggg tcttagtttt aaagaacgat tgctaggagg atggaaacat
     2101 gaaaaaaaat agtttatata tcatatcctc actctttttt gcttgtgtct tatttgtcta
     2161 tgctacggcg acgaattttc aaaacagtac cagtgctagg caggtaaaaa cggaaaccta
     2221 tactaataca gtaacaaatg tccctattga catacgctat aatagtgata agtattttat
     2281 tagcggtttt gcttcagaag tatcagtggt cttgactggt gcaaatcgcc tatcgctagc
     2341 tagtgaaatg caagaaagta cacgtaaatt caaggttact gctgacctaa cagatgccgg
     2401 tgttggaacg attgaagttc ctttgagcat tgaagattta cccaatgggc tgaccgctgt
     2461 ggcgactccg caaaaaatta cagtcaagat tggtaagaag gctcagaagg ataaggtaaa
     2521 gattgtacca gagattgacc ctagtcaaat tgatagtcgg gtacaaattg aaaatgtcat
     2581 ggtgtcagat aaagaagtgt ctattacgag tgaccaagag acattggata gaattgataa
     2641 gattatcgct gtcttgccaa ctagcgaacg cataacaggt aattacagtg gttcagtacc
     2701 tttgcaggca atcgatcgca atggtgttgt cttaccggca gttatcactc cgtttgatac
     2761 aataatgaag gtgactacaa aaccagtagc accaagttca agcacatcaa attcaagtac
     2821 aagcagttca tcggagacat cttcgtcaac gaaagcaact agttcaaaaa cgaattaaaa
     2881 atagaaaagg attttataac aatgggtaaa tattttggga ctgatggagt ccgtggagaa
     2941 gctaacctag aactaacacc agaattagcc tttaaactag gacgttttgg aggctatgtt
     3001 cttagtcaac atgaaacgga agcgccgaaa gtctttgtag gacgtgacac acgtatttca
     3061 ggggaaatgc tggaatcggc cttggtggca ggtctccttt cagtagggat tcacgtatac
     3121 aaacttggtg tccttgcaac accagcagta gcttacttgg ttgaaactga aggagcaagt
     3181 gccggtgtca tgatttctgc tagccacaac ccagcccttg ataacggaat caagttcttt
     3241 ggcggtgatg gcttcaaact agatgatgaa aaagaagcag aaattgaagc cttgctagat
     3301 gctgaggaag acactcttcc tcgtccaagt gcagaaggct taggaatttt ggtagattat
     3361 ccagaaggct tgcgtaagta tgaaggatac cttgtttcaa ctggaactcc tcttgatgga
     3421 atgaaggttg ccttggatac agctaatgga gcagcttcta ccagtgcccg tcaaatcttt
     3481 gcagaccttg gtgcccaatt gacggttatc ggggaaacac cagacggtct taacatcaac
     3541 cttaatgttg gttcaacaca tccagaagcc cttcaagaag tggtcaaaga aagtgggtca
     3601 gctattggtt tggcctttga tggagacagt gaccgcttga ttgctgttga tgagaatggt
     3661 gacatcgtcg atggtgacaa gattatgtac atcatcggaa aatacctttc tgaaaaagga
     3721 caattggctc aaaatacaat tgtgacaact gttatgtcta accttggttt ccacaaggcc
     3781 ttgaatcgcg aaggtattaa caaggcagtt actgcagttg gtgaccgcta cgttgttgaa
     3841 gaaatgagaa aatcaggcta caaccttggt ggtgaacagt ctggtcacgt tatcttgatg
     3901 gattacaata ccacaggtga tggtcaatta tcagcagttc aattgactaa aattatgaag
     3961 gaaactggta agagcttatc agagttggcg gcagaagtaa cgatttatcc acaaaaatta
     4021 gttaatatcc gagtggaaaa cgtcatgaag gaaaaggcca tggaagtgcc agctatcaag
     4081 gccatcatcg agaagatgga agaagaaatg gcggggaacg gccgtatcct tgttcgtcca
     4141 agtggaacag agcccctctt gcgtgttatg gcagaagcgc ctacaacaga agaagtggac
     4201 tactatgttg ataccatcac agatgtagtt cgtgctgaaa ttgggattga ctaaatccta
     4261 gaaaactaga tgaaaataaa aaattatggt acaatagctt ataatattgt agccgatgga
     4321 gaaagacatg aatcttaaac gtgaacaaga atttgttagt cagtatcatt ttgatgcacg
     4381 taattttgaa tgggaaaatg aaaatggagc tcctgaaacc aaggtggatg tgaatttcca
     4441 attgctccaa catgaccaag aaaatcaagt gacttccttg attgtcatct tgagctttat
     4501 gattgtattt gataagtttg tcattagtgg aacaatctca caagtaaacc atatcgatgg
     4561 ccgtattgtc aatgaaccaa gtgaattgaa ccaagaagag gtggaaacct tggctcgtcc
     4621 atgtttgaac atgctcaatc gtttgactta cgaagtgact gaaattgcat tagacttacc
     4681 aggaattaat ttggagttct aatatgaaat tagctgtttt cacagattcc tctgcttatc
     4741 tcagtgaaga gaccttgcaa agagaagact tgtttgtctt ggatattcct gtcaatattg
     4801 atggtgagga atatgtcgaa ggcatcaatc tgtctgctga ggaattttac caaaaaatgg
     4861 ctcaggcttc tgaattgcct aagaccagtc aaccaagtat tgccaagtta gatgagatct
     4921 taacttcgct caaagaacaa ggctatacac atgccttggg gcttttccta tcttctggaa
     4981 tttcaggttt ttaccaaaat atccagtata tggtcgatga ctatgagggc ttaaccattg
     5041 ctttcccaga cactttgatt acaagtgctc ccctaggcat catggttgaa agcgtcttta
     5101 attggcgtga tcagggcgat gattttgcca gcattcagga taagctagcc attcaaatca
     5161 gccgtacgtc agcctttatc atggtagatg acttggatca tttggtaaaa ggtggacgcc
     5221 tttcaaatgg ggctgccatt ttgggcaatt tgcttagcat taagccaatc ctttatttta
     5281 acgatcaagg tgtgattgaa gtttacgaaa aagttcgtac tgaaaagaag gccaccaagc
     5341 gcttaattga aattatcaag gaaacaacgg cttcaggtca ataccgggtc attgtcattc
     5401 atgggaatgc tcctgaaaag gctgaagaat tgcgtcagca cttgcttgat tttggcttgg
     5461 gttcggatgt ttcacttgct acatttggta gtgtcattgg aacgcaccta ggagcaggaa
     5521 gtattgctct gggttatatt ccagtgattt agttggcgct tctaggctag ttaagatgcc
     5581 ttgtatctta gaaattgaac acggactacc tgcctcttga aaaaagatgc ctgtcttgcc
     5641 tttcgtggaa agtcagtgcc attccctatt tttcagaggc agctaaggtc ctttgtatct
     5701 tgataattga acacgcctgg aaccctgtgt gaaaaagata gttcttccaa gaagtagaca
     5761 cttcttgatt ggaattccta ttttcacttt gtgttcttac ggactttgta tcttagacag
     5821 gagtagagat gagtattcga gtaattattg ccggttttaa gggaaagatg ggccaggctg
     5881 cttgtcagat ggtattgact gatccagact tggacttggt ggcagttttg gatccttttg
     5941 agtctgagtc agaatggcag ggtattcctg ttttcaagga taaggctgat ttagctggtt
     6001 ttgaagcgga tgtctgggta gattttacta ctccagctgt tgcctacgaa aatacacgtt
     6061 ttgctcttga aaatggcttt gctccagtag ttggaacgac tggtttcacg agtgaagaaa
     6121 ttgcagagct aaaagaattt tctcgtgccc aagacttggg tggcctgatt gcccctaact
     6181 ttgccttggg tgctgtctta ctcatgcaat ttgcgacgca ggctgccaaa tatttcccaa
     6241 atgtggagat tattgagctc catcatgaca agaaaaagga tgctccgagt ggaacagcca
     6301 ttaaaacagc tgagttgatg gcagaggttc gagagtccat tcagcaaggc gcagcagatg
     6361 aggaagagct gattgctggt gctcgtggtg ctgactttga tggtatgcgc atccactcag
     6421 ttcgtttgcc aggcttggta gctcatcagg aagtcatctt tggcaatcag ggagaagggc
     6481 tgaccctccg tcatgactcc tatgatcgca tctccttcat gacaggagtc aatttgggaa
     6541 ttaaagaagt tgtcaagcgt catgagcttg tctatggatt agaacactta ttatgagatt
     6601 aacgcaaatg ccttctgaat ttcagaaggc tttaccagta ttagaaaaaa ttaaagaagc
     6661 aggctttgag gcttattttg ttgggggctc tgttcgagat gcccttctcc atagccctat
     6721 ccacgatgtg gatattgcga cgtcttctta tccagaagag accaagcaga tttttccgcg
     6781 aacagccgat atcggaatcg agcatggaac cgtcttggtc ttagatgggg atgaggagta
     6841 tgaggtaaca acctttcgga cagaggatgt ctatgtggac tatcgcagac ccagtgcggt
     6901 ttcctttgta cgatcgctag aagaagacct caaacgccgt gatttcacag tcaacgcctt
     6961 tgccttggat gagacaggag aaatcgttga cttgttccat ggtttagaag atttggaaaa
     7021 gcaagtcttg cgagcagttg gagtggctag tgagcgtttc aacgaagatg ctttacggat
     7081 tatgcgtggt ttccgttttc aggctagtct tggttttgca cttgagccag aaacatttaa
     7141 agctatgaag accttgacgc cgcttttgga gaaaatttct gtagagcgta ccttcgtcga
     7201 gtttgataaa ctcttgctgg ctccattttg gagaaggggc ttggcttcca tgattgagag
     7261 tcaagcttat gactatctcc ctgatatggc atctagccag gacaagctca acagactgtt
     7321 tgatttggag actgatttta cttttgaatc ctctgaacaa gcctgggcgg ctttactatg
     7381 ggctttggag attgaaaatg cgcagtcact tttgaaatct tggaagacct cacgccagtt
     7441 tgccaagcaa gttcaggatt tgctgattat tttggctctg cgtgaaaatg gagaattgag
     7501 caagcgagat tgttatcgct ttgacataga tttgctttta caggctgaaa atcttcgtca
     7561 agctcaagga aaagaagtca acccacaagc catcacagaa aaataccaaa gcttgactat
     7621 ccatgacaag aaagagattc agattaatgg cggtattttg atcaaggaat atggctatca
     7681 gccaggccca gacttgggag agattttaac agagattgag tttgccattg tcgatggaga
     7741 attggagaat aatcgtgaag ccatccatgc ttacctgagg gagaaaaaat gagtgatttt
     7801 atcgttgaaa aactaagtaa atccgttggt gacaagaccg tttttaggga tatttccttt
     7861 attatccatg acttagacag aattggttta atcggtgtca atgggactgg caagaccacc
     7921 cttttggacg tcctttctgg tgtttctgga tttgatgggg atgtcagtcc tttttcagct
     7981 aaaaatgatt accagattgg ttacttgact caggatcctg attttgatga tagaaagaca
     8041 gttttggata cggttctatc tagtgaactc aaggaaatcc agctcattcg tgagtatgaa
     8101 ttgattatgc tcgactatag tgaggacaag caggcgcgtt tggaacgtgt catggcagag
     8161 atggactctc tccaagcttg ggaaatcgaa agtcaggtca agaccgttct tagcaaattg
     8221 ggcattcaag acttatctac tcctgttggg gaattgtcag gtggtctgag aagacgggta
     8281 cagttggcac aagtcttact tggcaaccac gacctcttgc ttttggatga gccgaccaac
     8341 catctggata ttgcgattat tgagtggctg accctctttt tgaaaaattc taagaagacc
     8401 gtccttttta tcactcacga tcgttatttc ttagacgctt tgtcaacacg gattttcgag
     8461 ttggatcgtg caggcttgac cgagtaccag ggaaattacc aggactatgt tcgcctaaag
     8521 gcggaacagg atgagcgcga cgcggctctt cttcacaaaa aagaacaact ctacaaacaa
     8581 gaattggcct ggatgcgcag acaaccgcag gcgcgtgcga ccaagcagca ggcccgtatc
     8641 aatcgattcc atgatctgaa aaaggaagtt tcaggcagta gtgctgagac agacttgacc
     8701 atgaactttg aaaccagtcg gattgggaag aaagtcatcg agtttcaggc tgtttccttt
     8761 gcctatgaaa acaagcctat tttgcaagat tttaatctct tggtgcaggc caaagaccgt
     8821 atcggaatcg ttggggacaa tggtgttgga aaatcaaccc tgctcaactt gattgcagga
     8881 agtcttgagc caactaaagg acaagttgtg attggggaaa ctgttcgcat cgcctatttc
     8941 tctcagcaaa ttgagggttt ggatgaaagc aagagagtta tcaattacct gcaggaagtg
     9001 gcagaagagg tcaagaccag tggcggttct acgacttcga ttgcagagtt gctggagcag
     9061 ttccttttcc cacgttcgac gcatggaacc ttgattgaga aattgtcagg gggtgagaaa
     9121 aaacgtcttt atctcctcaa actgcttttg gaaaaaccaa atgttcttct tttagacgag
     9181 ccaaccaatg acctagatat tgcaactttg acagtcttag agaatttctt gcaaggtttt
     9241 gcaggtcccg ttttaacagt cagtcacgac cgctatttct tggataaggt agcgaccaag
     9301 attctcgctt ttgaggatgg caagattcgt cctttctttg gtcattacac cgactatctt
     9361 gatgaaaaag cttttgaaac agatatggcc aatcaagtgc aaaaggtcga aaaggaaaaa
     9421 gtggtcaagg ttcgagaaga caagaaacgc atgacctacc aagaaaagca ggagtgggca
     9481 agtattgaag gtgatattga aaccttggaa aaacgtatcg ctgctattga agaggaaatg
     9541 caggctaacg gctctgactt tggtaagctg gctactctcc aaaaagaatt ggatgagaaa
     9601 aatgaagcac tccttgaaaa atacgaacgc tatgagtatc tcagtgaatt tgatagttaa
     9661 gactattcga aaatcaaagt gcaaactatg tcggcttcac attgccgact caagtacagc
     9721 ctgtgaccgg cttcctaatt tgcactttga tttttattga gtataaaaga atagaaaaat
     9781 cccgtctcat gaaaggattt ctctattctt ttttggtttc ttgatgaaag atattttgcc
     9841 aggtttcgtg gcgacgccag atactggtat ggatgatacc atctaactca tagcagatga
     9901 gtttggtttt ttgactgatg gaagtaattt gaatgtcctt tatagcagaa tttaactctt
     9961 ttttggtctt ataagcctct ttatccatct gctctccatc ttggcgaata tagacaaaat
    10021 catcagtcaa gagttcttct agttggtttc cttggtcaat caattgttca cgcataagca
    10081 attttttata gacattgtct aaaatttcat cctcagggat aggtgctggt tcgatatgga
    10141 catcggtatc aaagacgcca aaacgctcct ccagcataga ctcgacctga tccgcgattt
    10201 catggctttc aaaaacagac aagtcaggat tcatctctag tgtaatatcc aggtagatgt
    10261 tgctaccgta ggtgcgacct ctttgcgatt tgaccttgct gattttggga atttccatga
    10321 tagccttttg gtagtcctcg agcaggcggt cgtcaaagcc atctgaaaga ctaaaggaag
    10381 actcgatgaa gatatcatag gcagtcttca agataaagaa agtgatgatg atagcaacca
    10441 gtttatccac aatcggataa ttgaaactac tagctaggat ggcaatggcg gttccaagtg
    10501 aggtaacagc gtcagaaaga ttgtccttag cagctgcctt cagcgccttg gagttggatt
    10561 tcttactgag gcgagtattg tagagataga ccacaaacat aatcgctgca gaaatgattc
    10621 ctagagttgc accaagagga tcaatgaccg tttcttcccg actgagaatc ttttgaatgg
    10681 tatctcttag aacatcgaaa ccgacataga acatgatgat agaagtgatc aagcttgcca
    10741 aatcttcaat cttccaatga ccaaaacggt ggtcacggtc tgcaggctgg cgcgccatcc
    10801 gaatcccgat taagagggcc acatttccaa tgatgtccga tacgttatta aaaccatcgg
    10861 ccaccaaact ggatgaatga aggagatgac cagctgctaa tttagctgca gacaagatca
    10921 aataggtcga aatactgata atggctccac gctcagctaa cttgagattt gagatagatt
    10981 gcttcattca acttcctttc tagtcacata gcattctacc attatactgg ttttcaagga
    11041 aaaattcaaa taaggcagtt tttactcttt gaaaactccc caaaatttgg tataatggta
    11101 ctactcaagg gagtagctgg cagaaacctg tgatagtgtc gtcattccga attttatgct
    11161 gaaaagtatg ctttccggcc ctatcttaaa cagcgagact tgttatgatt aacaggtctc
    11221 tttttgtttg tcgtgaaaag atatgacaga aagagagtct gttttgcaca caatgtcaag
    11281 gaggagacac atgtcaaaag aacaaaaacg ccaagcgttt tatactcaga gccctgaaga
    11341 ggtcttgcag gctgtggatg cgaccgagca aggtttgtca tcaagtgagg cggaaaagcg
    11401 ccttgccgaa tttgggcaca atgaactcga agaaggcgag aaacgatcaa tcttggtcaa
    11461 attcatcgag caatttaagg atttgatgat tatcatccta gttgcggcag caatcttgtc
    11521 agtcgtgact tctggtgggg aagatatcgc agatgccatt atcatcctag ctgtggttat
    11581 catcaacgct gcctttggtg tttaccaaga aggaaaagct gaagaagcta ttgaagccct
    11641 caaatccatg tctagtccag ttgcccgcgt tcttcgtgat ggacacatgg cagagattga
    11701 ctctaaagaa ttggtaccag gcgatatcgt tgcccttgaa gcaggtgacg tggtaccagc
    11761 ggacctacgt ttgatagaag ccaactctct taaaattgaa gaagcagcct tgacaggtga
    11821 atctgtacca gtcgaaaaag acttgtcagt cgagcttgcg acagatgctg gtattggtga
    11881 ccgtgtcaac atggccttcc aaaactcaaa cgtaacctat ggtcgtggga tgggtgttgt
    11941 tgtcaataca ggtatgtaca ctgaagttgg tcatattgct ggtatgcttc aagatgcgga
    12001 tgagactgat acaccactca aacaaaactt gaacaacctt tctaaggtct tgacctatgc
    12061 tatcttggtc attgcccttg ttacttttgt agtgggtgtc ttcattcaag ggaaaaatcc
    12121 acttggtgag ttgttgactt ctgttgccct tgccgttgca gccattccag aaggacttcc
    12181 tgctatcgtt accatcgttc tttctcttgg tactcaagtt ttggccaaac gacattccat
    12241 cgttcgtaag ttgccagcag ttgaaacact tggttcaact gaaatcatcg cttctgataa
    12301 gactggtacg ctgactatga acaagatgac agtcgaaaaa gtcttctacg atgcggttct
    12361 acatgactca gctgatgata ttgaactagg tcttgaaatg ccactacttc gttcagttgt
    12421 cttggccaat gatacgaaaa tcgatgtgga aggtaacttg attggtgacc caaccgaaac
    12481 agcctttatc caatatgcct tggacaaggg ctatgatgtc aaaggtttct tagagaaata
    12541 tcctcgtgta gctgaattgc catttgactc tgaccgtaag ctcatgtcaa cagttcaccc
    12601 attgccagat ggtcgtttcc ttgtagcagt caagggtgcg ccagaccaac tcttaaaacg
    12661 ttgtcttctt cgtgataagg ctggggatat tgctccgatt gatgagaagg ttacaaatct
    12721 cattcataca aacaattctg aaatggctca tcaagccttg cgtgtccttg caggtgctta
    12781 taagattatc gatagtattc cagaaaatct cacttctgaa gagcttgaaa atgatttaat
    12841 ttttactggt ttgattggga tgattgaccc tgaacgtcct gaagccgctg aggctgttcg
    12901 tgtggctaag gaagcgggaa tccgtccaat tatgattaca ggtgaccacc aagacacagc
    12961 ggaagccatt gccaaacgtt tgggaatcat tgacgcaaac gatacagaag gtcacgtttt
    13021 aactggtgct gaactcaatg aactgtcaga tgaagaattt gaaaaagtcg ttggtcaata
    13081 ctctgtttat gcccgtgtgt ctccagaaca caaggttcgt atcgtcaagg cttggcaaaa
    13141 acaaggtaaa gtcgttgcca tgacaggtga cggtgtcaat gacgcgccag ctctgaaaac
    13201 agccgatatc ggtatcggta tgggaatcac tggtacagag gtttctaagg gggcttctga
    13261 tatgattctt gcagatgata actttgcgac tattatcgtc gcagtggaag aaggacgtaa
    13321 ggtcttctca aacattcaaa agactattca gtacctactt tctgctaata ctgctgaagt
    13381 attaaccatc ttcctatcaa ccttgtttgg ttgggatgtc ttacagccgg ttcatctttt
    13441 gtggatcaac ttggtaacgg ataccttccc agctatcgct cttggtgttg aacctgcaga
    13501 acctggtgtc atgaatcata aaccacgtgg acgcaaggca agcttcttct caggtggtgt
    13561 tttgagttct atcatttatc aaggtgtact ccaagcagct cttgttatga gtgtttatgg
    13621 ccttgcgatt gcttacccag ttcatgtggg tgacaatcat gctattcatg cagatgccct
    13681 aacgatggcc tttgcaaccc ttggtttgat tcagctcttc catgcctaca atgtcaagtc
    13741 tgtctaccaa tccatcttga cagttggccc attcaagtct aagaccttta actggtccat
    13801 cttggtatcc tttatccttc tcatggcaac aatcgttgta gaaccgcttg aaggaatctt
    13861 ccacgtaacc aaactagact tgtcacaatg gggaattgtt atggctggaa gcttctcaat
    13921 gattatcatc gtcgaaatcg ttaagtttat tcaacgcaaa ctcggttttg acaagaatgc
    13981 gatttaaggg aggtttcgga tgtcagcata tcaattaccg accgtatggc aggatgaagc
    14041 tagtaatcaa ggagctttta cggggctaaa cagaccaaca gcaggtgccc gtttcgaaca
    14101 aaacttgcca aaaggagaac aagcttttca gctttattca ctgggaacac caaatggtgt
    14161 gaaggttact atcttattgg aagaattact agaagctggt tttaaggaag cggcttacga
    14221 cttgtataag attgctatca tggatgggga tcaattcggc tcagactttg tgaagctcaa
    14281 tccaaattcc aagattccag ccttattgga ccagtcaggt actgaaaacg taagagtctt
    14341 tgagtctgct catattcttc tttaccttgc tgagaaattt ggagcctttt taccaagtaa
    14401 tcctgtggaa aaggtagaag ttttgaattg gctattctgg caagcaggtg cagcaccttt
    14461 tctaggtggg ggatttggac atttcttcaa ttatgctcct gaaaaattgg aatatcctat
    14521 taaccgtttt acgatggaag tgaaacgcca gttggattta ttggataagg aattggctca
    14581 gaaaccttat attgcaggca atgactatac gattgcagat attgctatct ggtcttggta
    14641 tggacagtta gttcaaggaa atctttacca aggttctgca aaattcttgg atgcctcaag
    14701 ttatcaaaat ctagtaaaat gggcagaaaa aattgccaat cgtccagctg ttaagcgtgg
    14761 attggaagta acttatacag aaattaaata gcaaaaaagt catcttcgga tggctttttg
    14821 ctacagttga gggaaagggt ttgcagttat cggcttttgt gctataattg ctataatata
    14881 gtaaaaagca agaggagtgt gaggaagtgg aaaagaaaat tgtgaaggat atcttatttt
    14941 tatctcaagt gtctcagccg gcaagtcagg aggaccttta tcttgccaga gatttgcagg
    15001 atacactctt agcaaatcgt gatacctgtg ttggtctagc tgccaatatg attggggtgc
    15061 agaagcgcgt gattatcttt aatcttggct tggttcccgt ggtcatgttt aacccagtgc
    15121 ttctgtcctt tgaaggatct tatgaggcag aagaaggctg tttgtccttg gtaggtgtga
    15181 gatcaactaa gcgttatgaa accataaggc ttgcctatcg tgacagcaag tggcaggaac
    15241 agaccattac cttgacaggc ttcccagctc agatttgcca gcatgagctg gatcacttgg
    15301 aaggacgaat catttaggag gaaagcaaat gaaacgaata gtctttgaac ttatttttat
    15361 cgcaacgacc tggtatatct ttttaccgcc ccttaacctg accagctggg aatttctctt
    15421 cttcctctgt gggcatttgt tagttgtggc aatcttattt ggctttggca aggggataaa
    15481 ccttgtcaaa acggttcatg tgcgccacgg taaggcggaa gctgccttaa atcttgaggg
    15541 tttcaaaatc aatcggttag ggaaaattct gttagcttcg attggaggaa ttcttctctt
    15601 ggcagctttg gtttccttgg taacttccag catgtttcag gctaaaaatt atgccaatgt
    15661 agtcacagtt acggaaaaag actttactga atttcctaag agtgacacca gtaaggttcc
    15721 tatcctagat agaagtactg ctgaaaaaat tggagaccgc tacttgggtt ccctaaccga
    15781 taaggtgtcg caatacgtag cggcagatac ctatacccaa ttgacaattg atgggaaacc
    15841 ttatcgggtc acaccactag aatatgcaga ccctatcaaa tggtttaaca atcaagccaa
    15901 gggaatcggt gagtatatta aggtggacat ggtaactgga aatgcggatt tggtggactt
    15961 gaagacacca atcaagtatt cagactcgga gtattttaac cgtgatgtca aacgtcacct
    16021 gcgcttgaag tacccgacca aaatctttaa aactccatct tttgaggtgg acgatgaggg
    16081 caatcctttc tatgtagcaa cggtttacca aaagcaattt ggacttgctg ttcctcgtcc
    16141 tgcttcagtc attatcttgg atgctacaaa tggagaaacc aaggaataca gcttatcaga
    16201 tgttccagaa tgggtggaca ggatctatcc agcagaggaa accattgagc aaatcaacta
    16261 caacggcaag tacaaggacg gtttcttgaa tgccatgatt tccaagaaaa acgtgaccca
    16321 gactaccaat ggctataatt acttgtctat cggtaatgac atctatctct acacaggtgt
    16381 gacgtcggct aatgcggatg agagtaatct tggtttcatc cttgaaaata tgcgaacagg
    16441 agaaatcact aagtatagct tggcttctgc gacagaagaa tcagcccgtg aatcagcaga
    16501 aggtgctgtt caggagaaat cctacaaagc aaccttccca atcctcatca acctcaatga
    16561 caagcctctc tacatcatgg gcttgaagga caatgctggc ttggtcaaag agtacgccct
    16621 ggtagacgca gtcgagtacc aaaatgttat cgttgctact acagtggaag agatgctcag
    16681 caagtatgcc aataaaaacg accttgaaat tgacaatgca acgacagaaa gcatcaaggg
    16741 agtagtagca gacctcaaat cagctgttat caagggagac actgtctact tctttaaagt
    16801 tgatggcaag atatacaagg tcaaggcttc agtatccgat gaccttcctt accttgaaaa
    16861 tggtaaaacc ttcgaaggtc aagtaggaaa agacaattat ctcaagacct ttaagctacg
    16921 gtaaaaatag gttttttcag aaagtatatg ttataataga gtaaattaag actaatggag
    16981 gacaaagaaa tgggttttta tttgatggtt gcttcaatgc tactcggact gctcgctttg
    17041 aagataggtt tttctcagtt caaggagaaa aaagataaat tcttatctat cttaacaagt
    17101 ttagctggca cagcccttgt tctcgtggct gtctggttag gatggcccaa ataaatagaa
    17161 aaacctcggt caaaccgagg tttttcaaat gaatttcttg gttgtggcta aaaaatatgc
    17221 tacactatca atatgaaaat tttaatccca acagcaaaag aaatgaacac agacttgcca
    17281 agtatcgagg caattccttt aaaaccagaa agtcagaccg tgcttgatgc cttggctctc
    17341 tattctgcca gtcaattgga gagtttctac aaggtatcag ctgagaaagc ggcggaagaa
    17401 tttcaaaata tccaagcttt gaaaaggcaa actgctcaac actatccagc cttgaaactt
    17461 tttgatgggc ttatgtaccg caacattaag agagataagc tgaccgaggc ggaacaagat
    17521 tatcttgaaa atcatgtctt cattacctcg gctttgtacg gtgttgttcc agccttgtca
    17581 cccatggctc ctcaccgttt ggattttttg atgaaattaa aagtcgctgg taagactttg
    17641 aagagccatt ggaaggcagt ctatgatgaa gctctgaaga aggaagaagt gattttctct
    17701 ctcttgtcat cagagtttga gactgtattt tctaaggaaa tcagagcaaa gatggtgacc
    17761 ttcaaattca tggaggatag aggcggtcag ctgaagattc actcaactat ctccaagaaa
    17821 gcgcgcgggg cctttctaac agctttaata gaaaatcaag tacaaactgt gggggaagca
    17881 cgtcgcttga actttgctgg atttgtttac cgagaagatt tgtcacaacc acagggattg
    17941 gtttttgtaa aggaagtata gaaatcagat aagagaaaag tcagcacgat tatgctgact
    18001 tttttgataa cttataaagt attggacaaa tgaatctcat ttgataaggt gttaagatga
    18061 ttaaccaaat ttatgattga cagatgattt cggcaaataa acttcttggt aatccctgga
    18121 atatagaaat tagacacgat gatatcgtaa tctgtctgat ttaaaatctc aggacttgtt
    18181 tttaattcat cccaaatatc gaaggtaaag cggttattac agtaatagga aagcatatca
    18241 acaaaggtaa gggatatagc atggtcaaag ttactgataa tcaaaacctt gataggtggt
    18301 cgattttcta acaactgagt agagaggttt tcagcatggg ttaagatggt gtacatcaag
    18361 tgttccaact gttcaggatg gtcatgctgt ccaattgcct gacggtattg agccaattct
    18421 tgacgcgcac ttcccataaa gtctgggaag taaacttcaa attttttaat cgtcaatgct
    18481 ttttgttcaa ataagattgg ggtagaaaag atttcctgtc tttcaaagaa agcagtgttg
    18541 tgtaaatgcc aaatcagctc atcgtggtta gtaaaagtaa tcttatattg ttttgatagg
    18601 cgttctaata tttgactaag taattggtaa gagtatcttg cttgactgtt atcttctaaa
    18661 gagttgaaga attcttgagg atctaggaaa atatcattct gaagaaaaga aataaaaatt
    18721 tgagcgattg tatctggagt catttcaagc ccaaaatgtt tagagaaata agcaagtgtc
    18781 tcctggaaat ttggaatgct ctttaaaagc ggataatatt ctttgtaaaa gtggttgggg
    18841 aggtcaatga agtgaccatt tttaaccctg tggatactga tagctatcat cagtttgtac
    18901 attctataaa ttgaaaatcg cattggataa ttcgtgattt tatagaagaa gtcagcaaac
    18961 tctgttaaat cttcctcagg aaggtcagga aaaggccaat ctaggaaata atagcgctca
    19021 gaaaagtatt gggcaaaaaa gtaacgaata tcgatctcat ttccacgtat ctcggatgga
    19081 gtaaaagata attcaatttg aaactgagat gaaagaacct ttgtaatatt tctgcccagt
    19141 cgatagaggt tggcactact aaagtaaaga ttttctattg ttcgataaat aggtagcccc
    19201 tcattgaaga acatatactc tagtaattga aaagattgag aattagcaag aaaatattga
    19261 taaatatctt ctacactagt gttaacttct aaatctatca tgattccgtt aacagaagat
    19321 tggatgttaa tagatggaaa ggcaatacgt aattcattta aatcactttt taagattcgt
    19381 tctgtgcatc ctaatttttc ggctagttca tgtaatttca tccaattacg attctgaatt
    19441 aggttttcca ttaagcgtaa ttgtctttga acttttgtgg ataaaaggtt tctcatacgg
    19501 gtattttctc cataattcat tatatctttt tttgtctaag tagtataaaa aaattagagc
    19561 aagaaaattg
//
LOCUS       DAVLTM010000025        19278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLTM010000025 DAVLTM010000000
VERSION     DAVLTM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.025"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            414..797
                     /locus_tag="VYN16_000802"
     CDS             414..797
                     /locus_tag="VYN16_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1403360.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVFYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            849..1538
                     /locus_tag="VYN16_000803"
     CDS             849..1538
                     /locus_tag="VYN16_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403361.1"
                     /translation="MKKYQLLFKTSAILSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1580..1813
                     /locus_tag="VYN16_000804"
     CDS             1580..1813
                     /locus_tag="VYN16_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403362.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1964..2575
                     /locus_tag="VYN16_000805"
     CDS             1964..2575
                     /locus_tag="VYN16_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403363.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2736..3530
                     /locus_tag="VYN16_000806"
     CDS             2736..3530
                     /locus_tag="VYN16_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1403364.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3527..4162
                     /gene="trmB"
                     /locus_tag="VYN16_000807"
     CDS             3527..4162
                     /gene="trmB"
                     /locus_tag="VYN16_000807"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1403365.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4289..4768
                     /gene="rimP"
                     /locus_tag="VYN16_000808"
     CDS             4289..4768
                     /gene="rimP"
                     /locus_tag="VYN16_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1403366.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4812..5948
                     /gene="nusA"
                     /locus_tag="VYN16_000809"
     CDS             4812..5948
                     /gene="nusA"
                     /locus_tag="VYN16_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1403367.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5970..6263
                     /locus_tag="VYN16_000810"
     CDS             5970..6263
                     /locus_tag="VYN16_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1403368.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6256..6555
                     /locus_tag="VYN16_000811"
     CDS             6256..6555
                     /locus_tag="VYN16_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1403369.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6572..9364
                     /gene="infB"
                     /locus_tag="VYN16_000812"
     CDS             6572..9364
                     /gene="infB"
                     /locus_tag="VYN16_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1403370.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9615..9965
                     /gene="rbfA"
                     /locus_tag="VYN16_000813"
     CDS             9615..9965
                     /gene="rbfA"
                     /locus_tag="VYN16_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1403371.1"
                     /translation="MVNHFRIDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10244..11023
                     /locus_tag="VYN16_000814"
     CDS             10244..11023
                     /locus_tag="VYN16_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403372.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            11025..11207
                     /locus_tag="VYN16_000815"
     CDS             11025..11207
                     /locus_tag="VYN16_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403373.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            11204..11344
                     /locus_tag="VYN16_000816"
     CDS             11204..11344
                     /locus_tag="VYN16_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403374.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(11550..11627)
                     /locus_tag="VYN16_000817"
                     /pseudo
     CDS             complement(11550..11627)
                     /locus_tag="VYN16_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11658..11891
                     /locus_tag="VYN16_000818"
     CDS             11658..11891
                     /locus_tag="VYN16_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1403375.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11891..13225
                     /locus_tag="VYN16_000819"
     CDS             11891..13225
                     /locus_tag="VYN16_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1403376.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13235..13489
                     /locus_tag="VYN16_000820"
     CDS             13235..13489
                     /locus_tag="VYN16_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1403377.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13585..13980
                     /locus_tag="VYN16_000821"
     CDS             13585..13980
                     /locus_tag="VYN16_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1403378.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLTLKGIGPATIKKLKENGIKFK"
     gene            14392..14841
                     /locus_tag="VYN16_000822"
     CDS             14392..14841
                     /locus_tag="VYN16_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1403379.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            14828..15388
                     /locus_tag="VYN16_000823"
     CDS             14828..15388
                     /locus_tag="VYN16_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403380.1"
                     /translation="MARTELPDKIETECLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYAFLKSEWEVI"
     gene            15388..15966
                     /locus_tag="VYN16_000824"
     CDS             15388..15966
                     /locus_tag="VYN16_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1403381.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            15959..18610
                     /locus_tag="VYN16_000825"
     CDS             15959..18610
                     /locus_tag="VYN16_000825"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1403382.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVVHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            18691..18855
                     /locus_tag="VYN16_000826"
     CDS             18691..18855
                     /locus_tag="VYN16_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1403383.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            18906..>19278
                     /locus_tag="VYN16_000827"
     CDS             18906..>19278
                     /locus_tag="VYN16_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1403384.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRS"
BASE COUNT         5803 a         3605 c         4385 g         5485 t
ORIGIN      
        1 agtatttctt gtagtgtcag tagataattt ttttagagac ttaaccccgt tactgttcat
       61 tttaaatatt attggcctgt cttgcttttc agtgctaaca tacattaaca taaaagagat
      121 actcttaaat ataagtaaat gatgatttgg ttagtcctag aaccaatcaa cttatgttta
      181 tcggcttggc agattttatg tttgtcattt gtttgtatag aggtatttca gaaacagaat
      241 tctatcaaca actaatcgct tatataggtg tattctctgc ttgtttatca cgcttctgct
      301 catgtggggc ttgaagtatt ataaaaagta gctttctatc cttttctgac aggatatttg
      361 agtagaaggc tctattaaat tgttagtgaa ataaatgaat ggaggtattt aatatgaaat
      421 acagattatt ttttgttatt ttcttgagta gtgtgttgga tattctttta gggacatttt
      481 tacaaatctc tatcgtatct attgggtggc ttgtttttta cagtggtttg tttgaagcag
      541 gagttttcct tcttgctaat aaaggggtgg cggtaaaaat caaggaagta gatattcgaa
      601 atcgctttaa atttattttt ggaaaaacct tatggtttca aattcttttg ctcatctttt
      661 tgataatcaa actttatctt ggtttggatg cgaggttgat tttattctat ggacatattt
      721 tcattgtctt taatgcctta atgtatctat tatctagtag tcaggttagc cttaaaaaaa
      781 acaaactgtc ttcttaatct tacagcagta gagcctgaca aggtgaggtc atagtaggga
      841 ggatttctat gaaaaagtat cagcttctat tcaaaacaag tgcaatttta tcctacctat
      901 ttttcgtatt tggtctttct cagctgacgc ttatcgtcca aaactattgg caattttctt
      961 ctcagatagg caatttattc tggattcaaa atatcttgag tttacttttt attggagtca
     1021 tgattgtggt tcttgttaag acaggccatg gttatctctt ccgcattcca agaaaaaaat
     1081 ggctttggta ttcgattttg acagtattag tggtagtgtt ccagatctct tttaacgttc
     1141 agacagctaa acatgttcag tcaactgcgg aaggttgggc tgtattgatt ggttatagtg
     1201 ggactaactt tgcagagcta ggtatttata tagccctgtt ctttctggtt ccactgatgg
     1261 aagaattgat ttatagagga ctactgcaac atgctttctt taagcattcg cgatttggtc
     1321 ttgatttgct tcttccttct attttatttg ctctccctca tttttcaagc ctgcctagtc
     1381 tgttagatat cttcgtcttt gcaacagttg gaatcatctt tgctggtttg acccgctata
     1441 ccaagagcat ttatccatcc tatgcggtgc atgtgatcaa taatattgta gcgaccttcc
     1501 cgtttttgct cacttttcta catagggtct tggggtaaaa aaacaaaagg cttgcttttc
     1561 agccatagag gaggtcatca tgtataaaca cttatttttc ctagattcca aaactttaga
     1621 tcggttgaca ccctatattc tagtcttggc ttctgacacc attgctttta atgtttttgt
     1681 gctaaccttt gtatctgcgg tggtttttaa tttcctaaat tccatgctag ctttaatggc
     1741 tatattcata ggggctggct atgtggtcgg attttggtta ctaatactca atgaaaatca
     1801 aagagcaaac taggaaacta gccgcaggct gtacttgagt acggcaaggc gacgttgacg
     1861 tggtttgaat ttgattttcg aagagtataa aatggtttgt tttggaaagg ctagaactaa
     1921 agaatgacgt gtaggaagtc tgtttgttga ggaggataat tttatggagt tttttgataa
     1981 atttcatgcc ttgtgttttg gatttttagt actaataatt gtcattacag ttccttatac
     2041 gattaaccat gggggttttt ttcaaaatga atctgcattg attcttgtaa gtcttcttgt
     2101 aacctcgctg agtgttgctt atgctagaaa gtttgaaatg atttcttttg ggatgttaag
     2161 caagaaacaa cttttgcttt tcattgcaat ctttcttcta agtgtacttg agacgctggt
     2221 ttatattcat ttcttcgctg tttcttctgg ctcaggggtc caacacttgg cggaagtcag
     2281 cagaggaatt tccctgtctt tgattttaac tacctcagtt tttggtccca tccaggagga
     2341 actcattttc agaggacttc ttcaaggtgc ggtttttgac aattcttggt tagggcttgt
     2401 gctaacttcc tctctctttt ctttcatgca tggaccttct aatgtccctt cgtttatttt
     2461 ttatctactt gggggtttgt tgctgggctt tgcttataaa aagagtcaaa acctatgggt
     2521 ttctactcta gtccatatgc tttacaacag ttggccactc ttatattatt tataaaaatt
     2581 atgaaaaggt aagcagaaga cggtgcttac ctttttcaag ctataatgat acccaagcca
     2641 atccagaggc ttttctttga gaatcgggtt agagtggttg acgaataggc caaaaactag
     2701 tagaatagta aggaaacttt atacggagga aagaaatgga tttgggtgat aatgagctaa
     2761 cactgactcc catacctggg aaaagtggca aggcttatat gggtagctat cctgatggga
     2821 agcgcatctt tgtaaaaatg aacacctctc caatcctacc tggtctagct agagaacaaa
     2881 ttgctccaca attattatgg agtcgtcgtt tggcagatgg gcgtgatatg tgtgctcaag
     2941 aatggttgac aggcaagata ttgaccccct atgatatgaa tcgtaagcaa atcgtcaata
     3001 ttttaacccg tcttcatcgc tcacgtccgt tgatgacaca attgagtcgt ttgggctatg
     3061 ccatggaaac acctgtagat ttactacagt cttggcagga aacggctcca gatgctttgc
     3121 gtaaaaatca ttttatcagt gaagtgatgg ctgatttacg tcagactatt ccaggattta
     3181 gagaggacca tgcgaccatt gtccatggag atgtacgaca tagtaattgg attgagacag
     3241 atagtggctt gatttattta gtagattggg attcggttcg cttgaccgat cgcatgtttg
     3301 atgtggccca tatgctctgc cattatattt cagaacatca gtggaaggaa tggttgacct
     3361 actacggtta caagtacaat caaacggtat taagtaaatt gtattggtat ggtcaattgt
     3421 cttatttgag tcagatttcc aagtattata tgaaccaaga tttagaaaat gtcaatcggg
     3481 agattcatgg tttgcgtcat ttccgagaca agtatggaaa gagaagatga gagttagaaa
     3541 tcgtaaaggg gcaacagaat tactagaggc aaatccccag tatgtggtcc tcaatccctt
     3601 ggaagccaag gcaaaatggc gggacttgtt tggcaatgat aatcccattc atgtggaagt
     3661 tggaagtgga aagggtgcct ttgtttcagg tatggccaag caaaaccctg acatcaacta
     3721 tatcgggatt gatattcaaa agtctgtttt gagctacgct ttggacaagg tgcttgaagc
     3781 tggagtgtct aacatcaagc tcttgtgggt agatggttct gacttgactg actattttga
     3841 agacggtgag attgatcgct tgtatctgaa cttttcagat ccttggccta aaaaacgcca
     3901 tgaaaagcgt cgtttgactt acaagacctt cttggatacc ttcaagcgta tcttgcctga
     3961 aaatggagaa attcatttca agacggataa ccgtggcttg tttgagtata gcctagtgag
     4021 cttttctcaa tatggcatga aactcaatgg tgtctggcta gatttgcatg ccagtgattt
     4081 tgaaggcaat gtcatgacag aatacgagca aaaattctcc aacaaggggc aagttatcta
     4141 ccgagttgag gcagaatttt aagaaatagc ctgaaatcag gctgtataag tgcttttgct
     4201 ttacataagt tagcaaatgt gctatactaa gggtaagaat atgagaaaga gaggcgggga
     4261 aatatcttcg cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg tagaattagt
     4321 cagagaagtt gtagaacctg tcatagaagc tcctttcgaa ctcgtggata tcgagtatgg
     4381 aaagattggc agtgacatga ttctcagtat ttttgtagat aaacctgaag gaattacctt
     4441 gaacgacacg gcagacttga cagaaatgat cagtcctgtc ctagacacca tcaagccaga
     4501 tcccttccca gaacaatatt tcctagaaat taccagtcca ggcttggaac gtcctttgaa
     4561 aaccaaggat gccgtcgctg gagcggttgg gaaatacatc catgtcgggc tctaccaagc
     4621 catcgataag caaaaggtct ttgaaggaac cttgttggcc ttcgaagagg acgagttgac
     4681 tatggaatat atggacaaga cacgtaagaa aactgtccaa attccatata gtttagtatc
     4741 aaaagcacgt ttagcagtta aattatagaa aaagaaagga tagcttttga ggattcaaaa
     4801 gtgaagaaaa catgagtaaa gaaatgctag aggccttccg cattttggaa gaagacaagg
     4861 gaatcaaaaa agaagacatc atcgacgcag tagtagagtc gcttcgttcc gcttatcgca
     4921 gacgctatgg tcagtcagac agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta
     4981 cagtttatac tgtccgtgaa gttgttgatg aagtatttga tagccgtttg gaaatcagct
     5041 tgaaagatgc tcttgccatt aattcagctt atgaacttgg agacaaaatc aagtttgaag
     5101 aagcaccagc tgagtttggt cgtgtagcag cccaatctgc caaacaaacc atcatggaaa
     5161 aaatgcgcaa gcaaacacgt gccatcactt acaatactta caaagaacat gagcaagaaa
     5221 tcatgtctgg tacagtagaa cgctttgaca accgctttat ctatgtcaac cttggtagca
     5281 tcgaagccca attgtcaaaa caagaccaaa ttcctggaga agtttttgct tctcatgatc
     5341 gtatcgaagt ttatgtttac aaggttgaag acaaccctcg tggtgtgaac gtctttgtta
     5401 gccgtagcca tccagaaatg atcaaacgtt tgatggagca agaaattcca gaagtttatg
     5461 atggaactgt tgaaatcatg agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg
     5521 ttcgtagcca caatccaaac gtggatgcta tcggtacaat cgttggacgt ggtggtgcta
     5581 atatcaagaa gattactagc aaattccacc cagctcgtta cgatgctaaa aatgatcgca
     5641 tggtaccaat cgaagaaaat atcgatgtta tcgagtgggt agcagatcca gctgaattta
     5701 tctacaatgc catcgctcct gctgaggttg accaagttat ctttgatgaa aacgacagca
     5761 aacgtgcctt ggtggttgtt ccagataaca agctttctct tgccattggt cgtcgtggac
     5821 aaaacgtgcg cttggcggct cacttgactg gttaccgtat cgatatcaag tctgctagcg
     5881 aatttgaagc catggaagac gctgcttcag tagagttgga agtagaaaac gatactgtag
     5941 aagaataaaa gctgctagag gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt
     6001 ctgttgtgtc taacgaagtg attgataagc gtgatttgct ccgcattgtc aagaacaagg
     6061 aaggacaagt ctttattgat cctacgggca aggccaatgg ccgcggcgct tatatcaaac
     6121 tagacaatgc agaagcccta gaggcgaaaa agaagaaggt ctttaaccgc agttttagca
     6181 tggaagtgga agaaagcttt tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa
     6241 gaagagagtt gggacttgaa taagcaaaag ataagtaatc tcttggggct tgctcagcga
     6301 gcagggcgca tcatatcggg tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc
     6361 aagttggtct ttctagctca tgatgctgga cccaatctga ccaagaagat tcaagataaa
     6421 agtcattatt atcaagtaga aattgtaacc gtgttttcaa cactggaatt aagcatagca
     6481 gtcgggaaat cgagaaaggt tttggctgta acagatgctg gatttacaaa gaaaatgagg
     6541 tctcttatgg aatagaagag gaggacatga tttgtctaag aaaagattgt acgaaatcgc
     6601 aaaagaactt ggaaaagaaa gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga
     6661 tgtgaaaagc cactcatcaa gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt
     6721 taagcctgca gctgctccga aagtagaagc aaaacctgca gcaccaaaag taagtgcaga
     6781 aaagaaagcc gaaaaatctg agccagctaa accagctgta gctaaggaag aggcaaaacc
     6841 ggctgagcca gttgctccga aaacagaaaa agtagcagcg aaaccgcaaa gccgtaattt
     6901 caaggctgag cgtgaagccc gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg
     6961 caataaccgt gaccaacaac aaaacggaaa ccgtcagaaa aacgacggcc gtaatggtgg
     7021 aaaacaaggt caaagcaacc gcgacaatcg tcgctttaat gaccaagcta agaagcagca
     7081 aggtcagcaa aaacgtagaa atgagcgccg tcagcaagag gacaaacgtt caaatcaagt
     7141 ggctccacgt attgacttta aagcccgtgc agcagcccta aaagcagagc aaaatgcaga
     7201 gtacgctcgt tcaagtgagg aacgcttcaa gcagtatcag gctgctaaag aagccttggc
     7261 tcaagctaac aaacgcaagg aaccagagga aatctttgaa gaagcggcta agttagctga
     7321 acaagcacag caagttcaag cagtggttga agtcgtccct gagaaaaaag aacctgcagt
     7381 ggatacacgt cgtaaaaaac aagctcgacc agacaaaaat cgtgacgatt atgatcatga
     7441 agaagatggt cctagaaaac aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca
     7501 aaagaatagt aactggaata acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg
     7561 taatcagact ccaaaacctg ttacggagcg taaattccat gaattgccaa cagaatttga
     7621 atatacagat ggtatgaccg ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga
     7681 aatcgttaag aaacttttca tgatgggtgt catggccaca caaaaccaat ccttagatgg
     7741 ggaaacaatt gaactcctca tggtggatta cggtatcgaa gccaaacaaa aggttgaagt
     7801 ggataatgct gacatcgaac gtttctttgt cgaagatggt tatctcaatg aagatgaatt
     7861 ggttgagcgt ccaccagttg ttactatcat gggacatgtt gaccacggta aaacaaccct
     7921 tttggatact cttcgtaact cacgtgttgc gacaggtgaa gcaggtggta ttactcagca
     7981 tatcggtgcc taccaaatcg tggaaaatgg taagaagatt accttccttg atacaccagg
     8041 acacgcggcc tttacatcaa tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt
     8101 ggtcgtagcg gcagatgacg gggttatgcc tcagactatt gaagccatca accactcaaa
     8161 agcagctaac gttccaatca tcgtagccat taacaagatt gataaaccag gtgctaaccc
     8221 agaacgcgtt atcggtgaat tggcagagca tggtgtgatg tcaaccgctt ggggtggaga
     8281 ttctgaattt gttgaaatct cggctaaatt caaccaaaat atcgaagaat tgttggaaac
     8341 agtccttctt gtggctgaaa tccaagaact caaggcagac ccaacagttc gtgcgatcgg
     8401 tacggttatc gaagcgcgct tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca
     8461 acaaggtacc ttgaatgttc aagacccaat cgttgtcgga aataccttcg gtcgtgtccg
     8521 tgctatgacc aacgaccttg gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc
     8581 tatcacaggt ttgaacgaag caccgatggc gggtgaccac tttgccgttt acgaggatga
     8641 aaaatctgcg cgtgcagcag gtgaagaacg tgccaaacgt gccctcatga aacaacgtca
     8701 agctacccaa cgtgttagcc ttgaaaacct ctttgatacc cttaaagctg gggaactcaa
     8761 atctgttaat gttatcatca aggctgatgt acaaggttct gttgaagccc tttctgcctc
     8821 acttcaaaag attgacgtgg aaggtgtcaa agtgactatc gtccactcag cggtcggtgc
     8881 tatcaacgaa tcagacgtga cccttgccga agcttcaaat gcctttatcg ttggtttcaa
     8941 cgtacgccct acaccacaag ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct
     9001 tcacagcatt atctacaagg ttatcgaaga gatggaagaa gctatgaaag ggatgcttga
     9061 tccagaattt gaagaaaaag ttattggtga agcggttatc cgtgaaacct tcaaggtgtc
     9121 taaagtggga actatcggtg gatttatggt tatcaacggt aaggttgccc gtgactctaa
     9181 agtccgtgtt atccgtgatg gtgtcgttat ctatgatggt gaactcgcaa gcttgaaaca
     9241 ctataaagac gacgttaagg aagttacaaa cggccgtgaa ggtggattga tgatcgacgg
     9301 ctacaatgat attaagatgg atgatgtgat tgaggcgtat gtcatggaag aaatcaagag
     9361 ataagatttt ttgctccttt cttaggtggt gagggacgca agcaaaccga tggtttcatt
     9421 gcttattttt gagcctaggg tctcaaaaat cccctgtgat gggactgata aatcagttcc
     9481 atcactttca ccacggcgaa agaagcagat gacttcaaat tgaacttcgt ttcaatttaa
     9541 actgaaaatc aagaagttta aaatagctag gtctgctggc ctagcttttg gttcaaagta
     9601 gagaaaggaa tatcatggta aatcatttcc gtatagatcg tgtgggcatg gaaatcaagc
     9661 gtgaagtcaa tgagattttg caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca
     9721 tcacagatgt tcagatgctg ggtgacttgt ctgttgccaa ggtttattac accattttga
     9781 gtaaccttgc ttcggataac caaaaagccc aaatcgggct tgaaaaagca actggtacca
     9841 tcaaacgtga acttggtcgc aatttgaaat tgtacaaaat cccagatttg accttcgtca
     9901 aagacgagtc catcgagtat ggaaacaaga ttgacgagat gctacgcaat ctggataaga
     9961 actaaagaag aggggttgcc cctctttttt ggtggaggaa aataggttga atttgaaatg
    10021 gaaaaatatt cttttataat agattgaaac tagaatagta cgcctctact tctaaaacat
    10081 tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttgtttc gttttaatat
    10141 aaaaaaggga ttctgtattt tttaatgtta tctaattaga aaatgctttt tttgtaggaa
    10201 atataatatg ataaggtgca aaaaagaaat aaggagtttg tatatggctg aacaagactt
    10261 agctatgcaa gtattgcaac aagtggtgaa actacctgtt gttaaggttg atcgttcgaa
    10321 atttttagtg gataagtttt ccaaagaatt gggtccacag gacattccta ccttattgga
    10381 acaaggtcca acgtctctct tatctcaaga aatcttagat cgagtggcta atgcctgtat
    10441 tagggataat gttttattag cgagtgggac ttctgttttg gcaggattac ctggagggct
    10501 tgctatggca attaccattc cagctgatgt ggctcaattt tatggtttct ctctgaaatt
    10561 ggcccaagaa ttaggttata tttatggtta tgaggatctt tgggcttcac gcgatgagtt
    10621 gagtgaagat gctcaaaata cccttttgct ttatctaggc gtcatgttgg gggtgaatgg
    10681 aactgctgct ttgctacgtg ctggtggtat aacaattgcc aaacaggtaa tgaaaacagt
    10741 acctaataaa gctttaacaa agacgctttg gtatcctatt ttgaaaaaag tcttaagaat
    10801 atttggtgtg aatcttacca agggagggct ggccaaagga atggggaaat ttattcctat
    10861 cttgggtggt atcatttcag gtggtttaac ctttgcaact atgaaaccga tgggtgaaag
    10921 cttgcagaaa gaattgtcca agctagttaa ctatagtgaa gttcaatatc aagaagatgt
    10981 tgaaacaatc cgaaaagagg ctgaaatcat cgaaggagag taatatggga ctcataaaaa
    11041 ctctagctaa aatttatggt aattactttt tgaccgtgca aggtgtaaaa gtaatgaaaa
    11101 cgataaagaa agatgataat gccgttgtcg gtctggggaa actttttatt gccgacaagt
    11161 taatggatac ggctcggtgg ctcattaagc cagaggataa aaaatgaaat ttttctgggg
    11221 acttcttgcc attattttta tcaaaccgat tattgggatt gtgaaattct tttggatgat
    11281 catctctttt gcagtccaat tgctgtttta caagatattg gattggttct ttaaacttat
    11341 ctagatggta atccaagtcg cagagaacta gcaggaactc cactgctagt tttttattct
    11401 ctttccatat ggtataatat aagcagtaaa atcattttat actcttcgaa aatctcttca
    11461 aaccacgtca gcttcacctt gctgtaggta tggttactga cttcgtcagt tctatccaca
    11521 acctcaaaac ggtgttttga gctgacttcg tcagttctat ctacaacctc aaaacactgt
    11581 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtattagaac
    11641 atacaatgga ggtcgtcatg gacaatatca tcgatgtgtc aattcctgtt gcagaagtgg
    11701 tggacaagca tccagaagtc ttggaaattc tagtggagtt gggttttaaa ccccttgcca
    11761 atcccttaat gcgcaataca gttggtcgta aagtatcact taaacagggt tctaagctag
    11821 caggaactcc tatggacaag attgtacgca cactggaagc gaatggctac gaagtgattg
    11881 gattagacta atgacagatg aacggattca tatcctacgg gatattttgt tagaattgca
    11941 caatggcgcc tctcctgagt cggttcaaga tcgctttgat gcgaccttta cgggcgtgtc
    12001 agccatcgag atttccctta tggagcacga gctgatgaac tcggattcgg gcgtcacttt
    12061 tgaagatgtt atggaactct gtgatgtcca tgccaatctt tttaaaaatg ctatcaaagg
    12121 tgtcgaagtt ttagataccg aacaccctgg gcacccagtt cgtgtcttta aggaagaaaa
    12181 tttggccctc cgtgcagctt tgattcgcat tcgtagattg ttagatacct atgagttgat
    12241 ggaagacgag gaaatgcttg cggagatgcg caagggcttg gtgcgtcaga tgggacttgt
    12301 ggggcaattt gacatccatt accaacgtaa agaagaactc ttctttccta tcatggagcg
    12361 ctatggacac gattcacctc ccaaagttat gtggggagtg gatgatcaga ttagggaact
    12421 ctttcaaaca gctctaacga cagccaagtc actaccagaa gtgtcaatta gcagtgtaaa
    12481 ggaagctttt gaagcttttg cgacagagtt tgaaagtatg attttcaagg aagagtccat
    12541 cctcctcatg attctccttg agtcttttac tcaggatgac tggcttcaga ttgcggagga
    12601 gagcgatgcc tatggctatg ccatcatccg tccgtcagag aaatgggtgc cagaacgaca
    12661 gagctttatt gaggaaaaga ttgcagagga gcctgtacag ctagatacgg cagaaggtca
    12721 agttcaacaa gtcatagata cgccagaagg ccattttacc attaccttta cccctaagga
    12781 aaaggaagct gtgctggacc gccatagtca acaggctttt ggtaatggct atctttcagt
    12841 cgagcaggcc aatctcatcc tcaatcacct ccctatggag attacctttg tcaataaaga
    12901 tgatattttc cagtattaca atgacaatac gccagctgat gagatgattt tcaaacggac
    12961 gccgtctcaa gtcgggcgca atgtcgaact ctgccatccg cctaagtact tggacaaggt
    13021 caaaactatc atgaaggggc ttcgtgaggg aagcaaagac aagtatgaaa tgtggttcaa
    13081 gtctgagtcg cgaggtaagt ttgtccacat cacctatgct gcagtacacg atgaagacgg
    13141 agaattccaa ggagtgttgg agtatgttca ggatatccag ccctaccgtg agattgatac
    13201 ggactatttt cgtggattag aataaggaga aaaaatgagt tacgaacaag aatttatgaa
    13261 ggaatttgaa gcttgggtca atacccaaat catgattaac gacatggcgc acaaggaaag
    13321 tcaaaaagtt tacgaagaag accaggacga gcgtgccaaa gatgccatga ttcgctacga
    13381 gagtcgcttg gatgcttatc agttcttgct tggtaagttt gaaaacttca aagcaggcaa
    13441 gggattccat gatttgccag aaggcttgtt tggtgagcga aattattaaa cgagaaagat
    13501 tcttgatttt tcactaaaat cttgatagaa tgtttatgtt aaatccttgt cagagcaggg
    13561 attttttatt gaaaggattt tatcatgtca aagaaactca atcgtaaaaa acaattacga
    13621 aatggcctcc gtcgcgcagg tgccttttca agtactgtga ctaaggttgt agatgagaca
    13681 aaaaaagtcg tgaagcgtgc agaacagtca gcaagcgcag ctggtaaggc tgtttctaaa
    13741 aaagttgaac aagcagtaga agctaccaaa gagcaagctc aaaaagtagc taattctgta
    13801 gaagattttg cagcaaactt gggtggactt ccacttgatc gtgccaagac tttctatgat
    13861 gaaggaatca agtctgcttc agatttcaaa aactggactg aaaaagaact ccttaccttg
    13921 aaaggaatcg gcccagctac cattaagaaa ttgaaagaaa atggcatcaa gttcaagtaa
    13981 ttttttttga gccttgcatt tccgagaaaa acttgctaca atagagccat tagaggtgtt
    14041 ttgaatccca cattttacag aaagtggcgg cgctgagaag tccacaaatg tgtcaaaact
    14101 ggttgctaat gactgaaaaa atgaaatatt tctgtctttt tattttaaag actaaagatg
    14161 cgggcttggc ccgaaattgg gtggtaccgc ggataaacac attcgtccct gtcatgtaga
    14221 tggcagggct tttcttttac gtcttagtca aagggggttg ttatgaagca atcttttaac
    14281 acaagtaaac tatattatgg ttttcctgtt tttattttag gatatcaaga ttagaatttt
    14341 ggctataaca tcacgacctg tagttcctct tatagtctag gagattgggt tgtgattgga
    14401 gtcgttgcga gagagaatgc cgcagagcag atcaaacagt atcaaaaatt tactgtgaat
    14461 atttctgatg aaacttctat gcttgcgatg gagcaggctg gttttatcag tcatcaggag
    14521 aaattggaac gtttgggagt gcattatgaa atttctgaac gaactcagac tcctatttta
    14581 gacgcctgtc cacttgtttt agattgtcgg gtagatagga ttgttgagga agacggtatt
    14641 tgccacatct ttgccaagat tcttgagcga cttgttgttc cagaactcct ggatgaaaag
    14701 ggacatttta aaaatcaact gtttgcccca acctatttca tgggagatgg atatcagcgc
    14761 gtttatcgct atctggacaa gcgtgtagat atgaagggca gtttcatcaa aaaagcgagg
    14821 aagaaggatg gcaagaactg agctaccaga taaaatcgaa acagaatgtc tcgttttacg
    14881 agtccgtact gtggcggatg ctgaggatat ctttgactat gctagtttgc cagaggtcgc
    14941 ctatccagca ggttttcctc cagtcaagac cttggaagat gagatttatt atctggagca
    15001 cattcttccg gagcgtaatc aaaaggaaaa tctcccagca ggctacggga ttgtcgtcaa
    15061 aggaacggat aagatcgttg gctctgtcga tttcaaccat cgccatgaag atgatgtgct
    15121 ggaaatcggc tataccttac acccagacta ttgggggcga ggttatgtgc cagaagctgc
    15181 gcgtgccttg attgacttag cctttaaaga tttgggtctt cacaagattg aactaacttg
    15241 ctttggatat aaccttcaaa gtcaacgagt cgcggaaaaa cttggcttta ccctcgaagc
    15301 tcgcataaga gaccgcaaag atgctcaagg caactgctgt gatgatttga gatatgcctt
    15361 tctgaagagt gagtgggagg tgatttgatg catcttttca tcattggtgc tccagcatca
    15421 ggaaaaatga ccattggtca agaattatct cgactgacgg atgctaccct cttttataac
    15481 catcaagcca ttgattttgc actagaaatc tatcaggact atacagagga aatgtgggaa
    15541 tttgttcgtg gaatcacttt ttcattcctt ggagcgagtg caagaaatca gcgatcagta
    15601 attttaacag atgtaattga tttttcaaat cagtatcatc tgatatattt aaagcaaatt
    15661 cagagtttgt tggatgatta tcatcaagag attctttttg ttgagttgga aacaagtctt
    15721 gaagagtgca tacgtcgaaa tcgaacggag aatcgattaa agtacaaacc cttaaaacga
    15781 catattgagg tatctgaaag agaaattata gagaccgctg aaacacttca attaaattcc
    15841 caacatcaac cgaatgagtt gaaccactac tttaaaataa ataatacgaa tttgtctgca
    15901 gaagaagctg ctaagcaaat tcaaaataaa ataaacaaaa tagagaaagg acacacacat
    15961 gtctaaagaa ctttcaccta aatacaatcc agccgaggtt gaggctggtc gttatcaaaa
    16021 atggcttgat gctgatgttt tcaagccttc aggcgatcaa aaggctaagc cttattcaat
    16081 cgttattcca ccaccaaacg ttacaggtaa acttcacctt ggtcacgctt gggatacaac
    16141 tttgcaagat attatcatcc gtcaaaaacg catgcaaggt tttgataccc tttggcttcc
    16201 aggaatggac cacgctggta ttgcgactca ggctaaggtt gaggagcgct tgcgtggtga
    16261 gggcatttcc cgctatgacc ttggtcgtga gtctttcttg acgaaagtct gggaatggaa
    16321 agacgaatat gccactacca tcaaggaaca atggggcaag atggggctct ctgtagacta
    16381 ttctcgtgag cgtttcactc ttgacgaagg tttgtcaaaa gctgttcgta aggtctttgt
    16441 ggacctttac aagaaaggct ggatctaccg tggtgagttt atcatcaact gggacccagc
    16501 agctcgcaca gccctttctg atattgaggt gattcacaag gatgtggaag gtgccttcta
    16561 ccacatgaat tacatgctgg aagatggttc acgcgccctt gaagttgcta caactcgtcc
    16621 tgagactatg tttggggacg ttgcggttgc ggttaatcca gaagacccgc gctacaagga
    16681 cttgattggt aaaaacgtca tccttccaat cgctaataaa ctcatcccaa tcgttggaga
    16741 tgagcacgca gatcctgagt ttggtactgg tgtcgtgaaa atcacacctg cccacgatcc
    16801 aaatgacttc ttggttggcc aacgtcataa cttgccacaa gtcaacgtca tgaacgacga
    16861 cggaactatg aatgagcttg cctttgaatt ttcaggcatg gatcgttttg aagctcgtaa
    16921 ggcagtcgtt gctaagttgg aagaaatcgg tgcccttgtc aaaatcgaaa aacgtgtcca
    16981 ttcagtcggt cactcagaac ggacaggtgt cgtagttgag ccacgcttgt ctactcaatg
    17041 gttcgtcaag atggaccaat tggctaagaa cgccattgcc aaccaagaca cagaggacaa
    17101 ggtcgaattc tacccacctc gtttcaacga taccttcctt caatggatgg aaaatgtcca
    17161 cgactgggtt atctctcgtc agctttggtg gggtcaccaa atccctgcct ggtacaatgc
    17221 tgatggtgaa atgtatgtcg gcgaagaagc tccagaaggt gacggatgga ctcaggacga
    17281 agacgtcttg gatacttggt tcagttctgc cctctggcca ttttcaacca tgggctggcc
    17341 tgaagtcgac tcagaagact ttaaacgtta tttcccaact tcaaccttgg taacaggtta
    17401 cgacatcatc ttcttctggg tgtctcgtat gatcttccag tcattggaat tcacaggccg
    17461 tcaaccattc caaaacgtcc ttatccacgg tcttattcgt gacgagcaag gacgcaagat
    17521 gtctaagtct ctcggtaacg ggattgaccc aatggatgtt atcgagaaat acggtgccga
    17581 tgcccttcga tggttccttt caaacggttc tgcgccagga caagacgtgc gtttctctta
    17641 tgagaaaatg gatgcttcat ggaacttcat taacaagatc tggaacatct ctcgctacat
    17701 ccttatgaac aatgaaggtt tgacgctgga tgtggtgcat gacaatgtca caaaagttgc
    17761 aacaggtgag gctggtaatg tgacggaccg ctggattctc cacaacctca atgaaacgat
    17821 tggaaaagca actgctaact ttgataagtt tgagtttggt gttgctggcc acatcctcta
    17881 caacttcatt tgggaagaat ttgccaactg gtatgttgag ttgaccaagg aagttcttta
    17941 cagcgacaat gaagacgata aggtcattac tcgatctgtt ctcctctata cgctggataa
    18001 aatcctccgt cttcttcacc caatcatgcc attcgtgaca gaagaaatct ttggtcagta
    18061 cgctgaaggc tctatcgtga cagcagcata cccaactgtc aacccagcct ttgaggacct
    18121 tgctgcccac actggtgtgg aaagtctcaa agacttgatc cgtgccgttc gtaatgcgcg
    18181 tgcggaagta aatgtagcac caagcaaacc tatcaccatc cttgttaaga caagcgatag
    18241 cgacttggaa gccttcttta acagcaatgt caactacatc aaacgcttta caaatccaga
    18301 acatttggaa atcgcatcaa ccatccctgc acctgaactg gctatgtcaa gtgtcatcac
    18361 aggagcagaa atctacctgc cactggccga tctcctcaat gttgaagaag aactggcccg
    18421 tctcgacaag gaacttgcta aatggcaaaa agaactggac atggttggta agaaactctc
    18481 taacgaacgc ttcgtagcca acgccaaacc agaagtcgtc caaaaagaat gcgacaaaca
    18541 agctgactac caagctaaat atgacgcgac cgtcgcacgt attgatgaga tgaagaagtt
    18601 ggtgaaatga tgttaaaaac ctaaccctct cgggttgggt tttacatttg ataatgttat
    18661 aataaaaagg agaaacttta ggagaatact atggggaaaa ttgttgctat tgatttattt
    18721 agcggtgcag gtggcactac aagtggcctt aaaaaatcag gcatagatgt tcaagttgct
    18781 gttgaaattg attccgtagc tgttaaaact tataagttaa ataatcctga ggtgtctgta
    18841 atagatatgg agtaaataaa acttcaattt tacaagcttt atatggttct ccagccaata
    18901 aaagtgtgtc tgaatattgg tttagtacaa atgttgatca aatagatgaa gtggatggga
    18961 aacaatgttt aatctattca tattataacg ttaaagcttc tagaaatgta gaagttctta
    19021 aaggccgttc tggaacaaaa aaaggattag attattggga accatatgct cctcaaaaac
    19081 aatatgagat ggaaagatta cccaaaaata aatatattgg aagttcttcg acagacagat
    19141 gggatggcat tgaaaaaaat gttgtctttt gtgattgtaa agaatatgtt tctgcttttg
    19201 atttattttt ctatcactat aattttaaaa aaatttctac tcaacgtagt aaacaagatt
    19261 ttataagatt acgatcta
//
LOCUS       DAVLTM010000026        18666 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLTM010000026 DAVLTM010000000
VERSION     DAVLTM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.026"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(235..1041)
                     /gene="yidA"
                     /locus_tag="VYN16_000828"
     CDS             complement(235..1041)
                     /gene="yidA"
                     /locus_tag="VYN16_000828"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1403385.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1054..2358)
                     /locus_tag="VYN16_000829"
     CDS             complement(1054..2358)
                     /locus_tag="VYN16_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1403386.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSISFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2430..2807
                     /locus_tag="VYN16_000830"
     CDS             2430..2807
                     /locus_tag="VYN16_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1403387.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWV"
     gene            2898..3230
                     /locus_tag="VYN16_000831"
     CDS             2898..3230
                     /locus_tag="VYN16_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1403388.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3242..4813
                     /gene="ffh"
                     /locus_tag="VYN16_000832"
     CDS             3242..4813
                     /gene="ffh"
                     /locus_tag="VYN16_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1403389.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5008..6291)
                     /locus_tag="VYN16_000833"
     CDS             complement(5008..6291)
                     /locus_tag="VYN16_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1403390.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6594..7307)
                     /gene="rsmG"
                     /locus_tag="VYN16_000834"
     CDS             complement(6594..7307)
                     /gene="rsmG"
                     /locus_tag="VYN16_000834"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1403391.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7401..7961
                     /locus_tag="VYN16_000835"
     CDS             7401..7961
                     /locus_tag="VYN16_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1403392.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7963..8862
                     /gene="htpX"
                     /locus_tag="VYN16_000836"
     CDS             7963..8862
                     /gene="htpX"
                     /locus_tag="VYN16_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1403393.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGACEVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGVQKLFYTHPPISERIERLKQM"
     gene            complement(8906..10465)
                     /locus_tag="VYN16_000837"
     CDS             complement(8906..10465)
                     /locus_tag="VYN16_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1403394.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYESYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTE
                     HGLELVNLEDV"
     gene            complement(10704..11021)
                     /locus_tag="VYN16_000838"
     CDS             complement(10704..11021)
                     /locus_tag="VYN16_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1403395.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            11191..11733
                     /locus_tag="VYN16_000839"
     CDS             11191..11733
                     /locus_tag="VYN16_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1403396.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11733..12362
                     /gene="nth"
                     /locus_tag="VYN16_000840"
     CDS             11733..12362
                     /gene="nth"
                     /locus_tag="VYN16_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1403397.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12573..13094
                     /gene="pyrR"
                     /locus_tag="VYN16_000841"
     CDS             12573..13094
                     /gene="pyrR"
                     /locus_tag="VYN16_000841"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1403398.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13113..14036
                     /locus_tag="VYN16_000842"
     CDS             13113..14036
                     /locus_tag="VYN16_000842"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1403399.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14086..15165
                     /gene="carA"
                     /locus_tag="VYN16_000843"
     CDS             14086..15165
                     /gene="carA"
                     /locus_tag="VYN16_000843"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1403400.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRDLTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15478..18654
                     /gene="carB"
                     /locus_tag="VYN16_000844"
     CDS             15478..18654
                     /gene="carB"
                     /locus_tag="VYN16_000844"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1403401.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTRLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAIAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5413 a         3597 c         4195 g         5461 t
ORIGIN      
        1 ttgaagagag tatttaaaat atcttgggaa attataattt ctccctgttt tttgagtcca
       61 atctatgagt atgagttgcc caggagtaag gccacattct aattcgttta ggtaaaaatt
      121 gttacaatca gaatttgtgt ctctattatg attattttgt tttaaattaa atagtttgct
      181 aaaaaaattc attggatttt attgctaatc aattcttatt gaaaaatgat acttttacag
      241 tacccatgtt cggatggcgt gggcaacgcc ggattcgtca tttgttttag tgatgtattt
      301 ggcgattttt ttgatttctg gatttccatt ttccatgaca acggggtttc caacgacttc
      361 cagcatggca cggtcatttt cttcgtcacc gattgccatg gtttcatctt tggtcaatcc
      421 tagtttttca gctaagtgag taatagctga acccttgtct acattctttt taaggagttc
      481 gaggtagaaa ggagcagatt tgttgataga gtagtgctcg taaaattcgg ctggtatttt
      541 ttcaatcgca gcatcgagga tttctggttc atcgatgaac atacacttga caatttcctt
      601 gccagccatt tcttcagggg tacggtagaa gataggcatg ctgacgaggg ttgattcgtg
      661 tacagtgtat tttccgatat tgcgatttgc agtatagata ccgtccttgg taatggcatg
      721 catgtggaca ccgagcttgc gactgaggaa ttccatatcc agataatcct cataggtcaa
      781 ggattcgctg ataatctcat ggccagtagc agtttcttgg acaagggcac cattgaaggt
      841 caccacatag tcaccttggt ctctcaactg caagtcgtcc agaagtttgg caacacctgc
      901 gatggggcgg ccagttgcaa tcacgacttt gacaccagct tctttggcat cttggatggc
      961 agaaaagact tcaggagtga tttccttttg gctgttgact agggttccgt caatatcgac
     1021 ggcgattagt ttaatactca taaatttcct cttttagttt ttatttgggg taaaatgatc
     1081 gttctcaatc aagtgtaaaa attgctgggt gatgcttgcg aagatgctgt tttggtctaa
     1141 catttctttc gggaaataaa agcgattatc tccgtggcga cttccagcaa gggattggac
     1201 gataggggac aggctagaga gttctgccag ttctccattt ttttgtaaaa tctcaatctg
     1261 tgtccgtggg ttttcagatt cgggacgata gatatcataa gggaggtcaa agttcttatg
     1321 aatggcagtg tagtagtcgg gatcaaagcc gatatcctca gctaattttc tcatgctggc
     1381 gagttggtct tgatcctctt gcgaaaagct aatggattta aagaccttgc ggttgacaaa
     1441 gcgttgcgat aaatctgcaa gaatcttgtc aggactggtc atccaaagct ggaagtaggt
     1501 attcatcacg ccatcatcca gagccagata gtcagtcaag gtcacatttt tttcgaagaa
     1561 aggcaggagg tgtggagaag ttcgggcaaa gaaatccttg tcctcaggat agagttcctt
     1621 ggcgcgtttg agaagattct gtaggagaac ttccatggcg cgtgttgcgg ggtggaaata
     1681 aacctgcatg tacatctggt agcgactgag gacgtagtct tcgatggcgt gcatgccatt
     1741 gcgctgaaag gcgataccat tttcgatagg acgaatgact cggaggattc gagtcaggtc
     1801 aaattcccca taggatgctc ctgtaaaata ggagtcgcgc aagagatagt ccatgcgatc
     1861 tgcgtcaatc tgactagaaa tgagctgcac gacctgctta ttaggatagg tatggtcaat
     1921 gacactggcc accttttctg ggaaatcagg tgccacttgt agcaggactt ggtgaatctc
     1981 tgtctcagga ttttgaataa tctcctgagt aatggcttca tggtctgtat caaagagatt
     2041 ttcaaaagta tgggagtagg caccatgccc aaggtcgtgt aggagagcag cggtcatggt
     2101 caagagagac tcggcaggat tccattcctc aggatatttt tcttcgaaaa tctctgtgat
     2161 gcgtcgtgca atttcataga ctcctagaca gtgagagaag cgactgtgtt ctccaccgtg
     2221 gaaggtataa ctggaagttc ccagttgttt gatccggcgc aaacgctgaa attcttttgt
     2281 attaatcaag tcatagatga tttgattatt gacatggatg tagttgtgaa cagggtcacg
     2341 gaatactttt tcgttcatat gaccattata gcaaaatcct gctctttttg gtaaaatagt
     2401 aggacaagag acaagaaaga gggcctgatg tgaagattcg gatgcgaaat acaattcagt
     2461 ttgatgagca gttggaagtg attgaccagc tttatgatgt ggaagtgcat gaaaaaggag
     2521 attatagcta cctgcttttc tataatgagg aaaaggaaaa agtagtgatt aaatttcatg
     2581 gtcaagaact tgtgatgagt cgattttcca atcccaagac cattatgcgc tttctaaagg
     2641 atagtgatag tttagcctat attcccacac ctatgggtat gcaggagttt accatccaaa
     2701 cgagccatta tcaagttgat aggcaaaaga ttgaactaga ttatcaacta caaaatcaag
     2761 agggacatcc ctttgccagc tatcaattgg aaattacttg ggtctaggct catgtctttt
     2821 tcaaatttag ctatctatct tcttggatcg gcttcctagt gtggtagatt aggctaattt
     2881 ttggtataat aaaagttatg gaaatcgaaa aaaccaatcg tatgaatgcg ctctttgaat
     2941 tttatgcggc gcttttgaca gataagcaaa tgaattatat cgagctctac tacgctgatg
     3001 attacagcct tgctgaaatt gccgaggagt tcggtgtcag tcgtcaggct gtctatgaca
     3061 atatcaagcg aacagaaaag attctggaag attatgagat gaaattgcac atgtactcgg
     3121 actatattgt ccgcagtcag atttttgatc agattttgga gcgctatccc aaggataact
     3181 ttctgcagga gcagatagaa attttaacaa gcattgataa tagagaataa gaggaagaaa
     3241 aatggcattt gaaagtttaa cagaacgttt gcagaacgtc tttaaaaatc tacgtaaaaa
     3301 aggaaaaatc tctgaatctg atgtccaaga ggcaaccaaa gaaattcgct tggccttgct
     3361 cgaggccgac gttgccttgc ctgttgtaaa ggactttatc aagaaagttc gtgagcgtgc
     3421 agtcgggcat gaggtcattg atacacttaa tcctgcgcaa cagattatta aaatcgttga
     3481 tgaggaactg acagccgttt taggttctga tacggcagaa attatcaagt cacctaagat
     3541 tccaaccatc atcatgatgg ttggtttaca aggggctggt aaaacaacct ttgctggtaa
     3601 attggccaac aaactcaaga aagaagaaaa tgctcgtcct ttgatgattg cggcggatat
     3661 ttatcgtcca gctgccattg accagcttaa gaccttggga caacagattg atgtgcctgt
     3721 ctttgcactt ggaacagaag taccagctgt tgagattgta cgtcaaggtt tggagcaagc
     3781 ccaaactaat cataacgact atgtcttgat tgatactgcg ggtcgtttgc agattgatga
     3841 gctcctcatg aatgagcttc gtgatgtgaa agcattggct caaccaaatg aaatcttgct
     3901 tgtcgttgat gctatgattg gtcaggaagc agccaatgtt gcgcgtgagt ttaatgctca
     3961 gttggaagtg actggggtca tccttaccaa gattgatggc gatactcgtg gtggtgctgc
     4021 tctgtctgtt cgtcacatta ctggaaaacc aatcaagttc actggtacag gtgaaaagat
     4081 tacggacatt gaaaccttcc acccagaccg catgtctagc cgtatccttg gcatggggga
     4141 tatgctcact ttgattgaga aagcttctca ggaatacgat gaacaaaaag cccttgaaat
     4201 ggctgagaag atgcgcgaaa acacctttga ttttaatgat ttcatcgatc aattagatca
     4261 ggtgcaaaat atggggccga tggaagactt gctcaagatg attccaggta tggccaacaa
     4321 tccagcactt caaaacatga aggtggatga acgccagatt gctcgtaaac gtgccattgt
     4381 gtcttcgatg acacctgaag aacgtgaaaa cccagatttg ttaaatccaa gccgtcgccg
     4441 tcgtattgct gctggttctg gaaatacatt cgtcgaagtc aataaattca tcaaggactt
     4501 taaccaggct aaacagctca tgcagggtgt tatgtctggg gatatgaata aaatgatgaa
     4561 gcaaatgggg attaatccaa ataaccttcc taaaaatatg ccaaatatgg gaggaatgga
     4621 tatgtctgcc cttgaaggaa tgatgggaca aggcggtatg cctgacttat cagctctcgg
     4681 aggagcagga atgccagata tgagccagat gtttggtggc ggtttgaaag gtaaaattgg
     4741 tgaatttgcc atgaaacagt ccatgaaacg tatggctaac aaaatgaaga aagcgaagaa
     4801 gaaacgcaag taagacaaga gattgatgca aatctcattt gatgagaaaa taaagtaaaa
     4861 atttctatga taaaacgtac agtatcaagg ttttagaata tctgatatta tacgtttttt
     4921 gattttaata attttgttaa gacactttta ttataatata cgaaaaatcc actcagctgt
     4981 ctgagtggat taggtgtatt tagactctta gtctttattt tcgtatggca agatcaagtt
     5041 caagatgatt cctgtcatgg ctgaaagggc agtacctgaa agtgtaactg gaccaagttt
     5101 aaggatagct cctccaagtc caagaaccaa catagcactt gcgatgatga ggtttcgcat
     5161 ttgagcgaaa tcaacacgtt ctttaatcaa gactttcaaa ccattgctgg cgataacccc
     5221 atagagaagg attgacatac caccaagtac agcgtttgga atagttgaaa tcaaggcagt
     5281 gaatttacca aggaagctga gggcaatcgc gatgaaggca gcgttacgga taactgagac
     5341 agaagcgata cgagtcatac cgataacccc tgtattttct ccgtaagttg tattggctgg
     5401 tccaccaagg aaggcagaaa cagaagttgc gataccgtca ccaagaagag tacggtgaag
     5461 acctggttct tttaagaatt gacgaccaca gatttgaccc aaaacagtat ggtctccgat
     5521 atgttcagaa attgttacga tagcgattgg caagatagcg atggcttctg gaccaaagta
     5581 aagattgtac tctttaaagg caccacctgt gctaaatggc aagtagaaac caggaatttc
     5641 gaaccagttg gctttaagaa ctggtgtaaa gtcaaccaag ccaagagtta gtgcgaaaag
     5701 gtaaccaccg ataatggcaa agaggaatgg aatgattcgt aggaagcctt ttccttttgt
     5761 attgataaag gcagcaatta ggaaagtaac aacggctacc agagcatttt tccaatttcc
     5821 gtctgctaca agacctgcat tggtaacagc tgaacctgca agtccaagac cgataacgat
     5881 gatcatagga ccgataatga ttggtggcaa gagtttatca atccattttg ttcctacaaa
     5941 tcggatgctg gtagcaacaa ggacatagac caaaccagtc aagataaccc ctgtttgggc
     6001 agcagataca tcccccccca tttctttcat agccagtgac atagctgtga taaaggcaaa
     6061 tgaagaacct agataaactg gaactttaaa accagtagca atcatgtaga tgagtgttcc
     6121 aacacctgaa gcaaaaaggg caacagatac aggcattccc aaaatcaatg gtaccaagat
     6181 ggtcgcacca aacatggcga aaacgtgttg gaaactaagg agaattcctt ttccatccga
     6241 aggacgttgg tcaacgtcta gtaacaaatc aacagttgat tcttgtttca tataaacctc
     6301 acttttgggc atcaaaaaag agcccattat tttacagcaa taggccctca gagtaagact
     6361 tctttaaaat ttagatttta aagagtttta aatatttctg cgtcttcgtc acctcacggg
     6421 atgacattaa aaacctttgc ttaggatagt atagcagaaa aaaatgattt tgtaaatcat
     6481 tttttcccga gcctagaaat aaaagagcga ggctgatttt gtaaatcatt ttttcccgag
     6541 cctaaaaata agagagcgag gttgattttg taaatatttt tttacttaaa aatttaaagc
     6601 gggcgtttat ttggcatacc agccttacgt ggatatttat ttggtgtttc ttttttcttt
     6661 tctaccactg tgatgtaacg cggatctcta tttggtaggg cgtagctgag attgtcttcg
     6721 actttactaa aaaggagatt gagggcattc ttagcttcta acaactcctc aggcgcattg
     6781 ctggccttga gtgccaatag ttttccacca accttaaggt agggaatagt caattcagat
     6841 aggacctgca tacgggcaac cgcacgagct gttacaaaat catattgagc acggaagttc
     6901 ttgtcttggg caaaatcttc ggcacgtccg tggtagaaat gaactccgtt caaatccagt
     6961 tcttgagcca agagttgtag gaagttgatg cgcttattga gtgaatcaat aatggtcaca
     7021 tctaactccg gatagagaat tttcattggt agactaggaa atcctgcccc agccccgata
     7081 tcaagaagtt tgatagtttc attgggaatc aaaccttgaa gaatgggtgc aatcgaatcg
     7141 taaaaatgtt tgagataaac ttcttccttg tccgtaatcg ccgtcaaatt aatcttctca
     7201 ttccactcga ccaagagctc aaaataacgt tcaaattgtt ctttttgctg gtccgaaagt
     7261 ggaagattct gctcggcaag caagttgtaa aatgtttttg gtttcatagt tatttccttc
     7321 tttagtatca tcttatttta ccacaatcat agaaaatttg atatactggt actaatctaa
     7381 aagcagaaag gacttatatt atgacttgga ttattcttgg agttttagct cttgttgtta
     7441 tttttgtgat tgttagctat aacggtttgg ttaaaaatcg tatgcaaacc aaggaggctt
     7501 ggagtcagat tgatgttcag ttgaaacgtc gcaatgatct cttgccaaac ttgattgaga
     7561 ctgtaaaagg ttatgccaaa tatgaaggtt ctacccttga aaaggtggca gaactacgta
     7621 accaagtggc ggcagcgact tcaccagcag aagctatgaa agccagtgat gccctcactc
     7681 gtcaggtttc aggtattttt gcagttgcag aaagctatcc agatttgaaa gctagtgcta
     7741 actttgttaa attgcaagag gagttgacaa acacagaaaa taaaatttct tactctcgtc
     7801 aactctataa cagtgttgtc agcaactaca atgtaaaatt agaaactttc ccgagcaata
     7861 ttatcgctgg aatgtttgga tttaaagcgg cagatttcct tcaaacacct gaagaggaaa
     7921 agtcggttcc taaagttgat tttagcggtt taggtgacta agatgttgtt tgatcaaatt
     7981 gcaagcaata aacgaaaaac ctggattttg ttgctggtat ttttcctact cttagctctt
     8041 gttggttatg cggttggtta tctctttata agatctggac ttggtggttt ggttatcgca
     8101 ctgattatcg gctttatcta cgctttgtct atgatttttc aatcgacaga gattgtcatg
     8161 tccatgaatg gagcgtgtga ggtggatgag caaacggcac cagacctcta ccatgtagtg
     8221 gaagatatgg ctctggtcgc tcagattcct atgccccgtg ttttcatcat tgatgatcca
     8281 gccttaaatg cctttgcgac aggttctaat cctcaaaatg cggctgttgc tgcgacttca
     8341 ggtctactag ctatcatgaa tcgtgaagaa ctagaagctg ttatgggaca tgaagtcagt
     8401 catattcgta attatgatat ccgtatttcg actattgcag ttgcccttgt tagtgctatc
     8461 accatgcttt ctggtatggc aggtcgtatg atgtggtggg gtggagcagg tcgcagacga
     8521 agtgatgatg accgagatgg aaatggtctt gaaatcatta tgctagtggt ttccctacta
     8581 gctattgtac tggcacctct cgctgcaacc ttggttcagc tcgctatttc tcgtcagagg
     8641 gaatttctgg cagatgcatc tagtgtcgag ctgactcgca atcctcaggg aatgattaat
     8701 gccctagata agttggacaa tagcaaacct atgagtcgcc acgtcgatga tgctagcagt
     8761 gctctttata tcaatgatcc caagaaaggt gggggggtcc aaaaactctt ttatacccac
     8821 ccacctatct cagaacggat tgaacgttta aaacagatgt aaaagaatcc agaggtcgtc
     8881 tctggatttt tgctatgttg caaatttata catcttctaa attaactaac tccaaaccgt
     8941 gttctgtcaa gcggtagatg cttgaacaga cctcttttaa gaaacgacgg tcatgcgaaa
     9001 cagtgatgag accgcctgga taggttgcaa agagttttct gatttggggt tgagaagtgg
     9061 gagaaaagtt tcgtgtgggt tcatccagca ggagaaagtt tggtttgcgc aggactaaat
     9121 ccaaaagcag gagttttccc tgttgtccgc cagataagga gcgaatttga tgctgcattt
     9181 ctggataact gaaattgaga ctagctaggt gagattggat tttctgtagt tcctcttttt
     9241 ccccagtttt gctgaggtag gctattggtg ataaatccaa ttgcagtttt ttgtggtaat
     9301 cttgtggcat aaaaccaagg gaaatctctc ttttatcatt cagaagttgc tttaatttgg
     9361 ctaacagagt tgatttccca acaccatttg gcccgataat accgattttt tcttgcccac
     9421 ggacagttag ttgtagtttt tgaaccaaaa ctcggtcgtc aatggacaaa ttttcttttt
     9481 ccagttggac taagacttta gaagctggta atggttggat gtctgaaaag aaaagttgga
     9541 tttgttcctc ttcaagtggc ttttgagtca tggactgagc tgccttttcg tagcgttttt
     9601 cttgtgagag gacagttttc atctttttag ccaataggcg accggcagta ctgtctttgg
     9661 tagctcgaag cgcagtttct acattttgtt tgactctccg atgtttttcc atggttttat
     9721 cgtaggctct ttggttgtta gcagcttgct gactttgttt ggcaaaatta gcctttctct
     9781 gctcactata gctttcatag tctaaatgct ctactagcgt ttccgcttct ttacggtgtt
     9841 tgaccagtcg caagtgaaca atagtgtctg ccgtttcaga aagaaagtct tcatcatggg
     9901 aaatgaaaat aacggtttgc ctggtctttt gaatctggcc ttttagccaa tcaactgtct
     9961 caaggtctag gtcatttgaa ggttcatcta aaaatagaat ctcaaagggt ttggctaact
    10021 catggataag ctgaattttc aaagcttctc cccctgatag attgccaatc ttttggtcac
    10081 ttgcgaaacg attgctatca aaatgcaatt cctccgccaa acgatagagg atactgtagt
    10141 ctaaatcaat agaatctaaa aagaagtagt cgtgtaaagt tttctttttc agctcctcgg
    10201 gaagtttttg aggaatgtag gccagtgact gatagtcaga ttggatgttt cccttgatag
    10261 tgaaatcaga caaagcttcc cccattaaaa ttttaagtaa ggttgatttg ccatttcctt
    10321 cttcaccaat aatagcaacc ttttccccgt cttggatggt catgcttagg tcagatacaa
    10381 gatctcgtaa atctttgttt tgtgtgatgg ttagatgatt gatttttatc atatgatttc
    10441 cctttctaga atgttcatag tgcataacaa aaagctctac tttacctagt agagcccaaa
    10501 gtctatgtca aaaaaactct attaacatgc cagaaaaaga gcataaaaga gcacacaaaa
    10561 agagacaaaa acaagatcta ctaaataaag gttctttatt tgatagactt agttgttcat
    10621 gtctccctta cctccgagta ttagtatctt tatattatac ctttaaggaa attttgtcaa
    10681 cagaaaactg gaaattctat gctctaaaat ccaaaaacag gtccatagag acttagtacg
    10741 tgtttgacat gctcgtaatg gaatttatca tagtttcgcc aagcagtgcg tgcttgatcg
    10801 tttagtttta ggctacccag tccaatcaga agactggtgg gttggtaaaa tttctcaaga
    10861 tcaattaaga tactgttgtt caacttttcc gtttttttga gctcttgcat agtagtgttt
    10921 agcagctctt gtagtcgaat atcatctgcc gtaccctgtt tacagttttg atagctttta
    10981 tttaacttgg ataggagttg aatgagtttc ttgtcttcca tgtgagcatc ctccttgatc
    11041 tgatgtactc tttccctata gtatagcact aaaaaacaaa actgtcatag taaatatgtt
    11101 aaaattttga attttaatta caaagtttgg tgtgctgatt tgtagccttt tcatggtata
    11161 atcaagagag taaatcttta tggaggaagt atgaaattaa atattcaaga aattcgtaag
    11221 cagtctgaag gtttgaactt tgaacaaacg ttagatttag ttgatgacct gcgtgcacgt
    11281 aatcaagaaa ttttagatgt aaaagatatc cttgcagttg ggaaagtaca atatgaagac
    11341 cgtatgtatt tcttagatta tcaactatct tataccattg ttcttgcttc gagtcgcagt
    11401 atggagccag ttgagttagt tgaatcttat ccagtcacgg aagttttcat ggaaggtgca
    11461 actaaccatc taaatcaaga aattttagat gatgacttgg tcttgcccat cgaaaatgag
    11521 gcgcttgacc ttgctgagag tgtatcagac aatatcctgc taaacattcc tatcaaggtc
    11581 ttgacggctg aagaagaagc tggtcaagga tttatctcag gaaatgactg gcaaatcatg
    11641 acagaggaag aataccaagc tcaaaaagca gtaaagaaag aagaaaacag tccttttgct
    11701 ggcttacaag gactatttga cggagatgaa taatggtctt gtcaaaaaaa cgagcacgaa
    11761 aggtgctaga agaaatcatt gccctcttcc cagatgccaa gcctagtctt gattttacca
    11821 atcattttga actcctggtt gcggtcatgt tatcagccca gacgacagat gcagcggtaa
    11881 ataaggccac accaggtctc tttgttgcct ttccaacacc ccaagctatg tctgtagcga
    11941 cagagagtga gattgcttca catatttctc gcttgggatt gtatcggaat aaagctaaat
    12001 tccttaaaaa atgtgcccaa cagttactag acgattttga tggtcaagtc cctcagacac
    12061 gtgaagaatt ggagagtttg gcaggtgttg gtcgcaagac agccaatgtt gtcatgagtg
    12121 taggatttgg gattccagcc tttgcagtgg atactcatgt ggagcgtatt tgcaaacacc
    12181 acgatattgt caaaaaatca gcgacgccac ttgaggtgga aaagcgggtc atggatatct
    12241 tgccgcctga gcagtggtta gctgcccatc aggccatgat ttattttgga agagccattt
    12301 gccatccaaa aaatccagag tgtgaccagt acccacaatt atatgatttt agcaatttgt
    12361 aagatgctac attgaaactt tttgcttgat tttaagcatg ctttgtgcta tagtaaatga
    12421 taactaaata ttcctttaaa cctgtcccgt gaggcaggca aggagcgcga taaagtagaa
    12481 gggtgaagtc tatactgtgt gcagtagggc tcgcttggct ataaatttca agtctccttg
    12541 tttaaggaga cttttctttt tggaggtttg tcatgaaaac aaaagaagtt gtagacgaat
    12601 tgaccgtcaa acgagcgatt acgcgtatta cttatgagat tatcgaacgc aacaaagatt
    12661 tgaataagat cgtcttggct ggtattaaaa ctcgtggtgt ctttattgcc caccgaatcc
    12721 aagaacgttt gaagcagtta gaaaatcttt cagttcctgt tgtggaattg gatactaaac
    12781 ctttccgtga tgatgttaaa agtggagaag atacttcttt ggtttctgtc gatgtgacag
    12841 accgcgaagt tatcttggtg gatgatgtgc tctatacagg tcgtaccatc cgtgctgcta
    12901 ttgataatat tgtcggtcat ggtcgtcctg cgcgtgtgag tttagcagtt ctagtcgatc
    12961 gtggacatag agaattgcca atccgtccag attacgttgg aaaaaatatc ccaaccagtc
    13021 gttctgaaga aatcatcgta gagatgacag aacttgatga ccaagacaga gttctgatta
    13081 ctgaagaagc ttagaaagtt aaaggagtag ccatgtcaga aaatcaacaa gcattgaacc
    13141 atgtggtgtc catggaagac ctcactgtcg atcaagtaat gaaattgatc aagcgaggaa
    13201 ttgagtttaa aaatggagcc cagcttccct atgaagacca tccgattgtt tccaatctct
    13261 tctttgagga ttctacacgg acacataagt cctttgaagt cgcagagatt aaacttggat
    13321 tggaacgact tgactttgat gtgaagacta gttcggttaa taagggtgag acactttatg
    13381 acaccatttt gactctgtct gctttaggag tggatgtctg tgtgattcgc catccagagg
    13441 tcgactacta tagagaattg attgctagtc caacgattac gacttccatc atcaatggtg
    13501 gagatggttc gggccaacac cctagccaga gcttgcttga tttgatgacc atttatgagg
    13561 aatttggcca ctttgagggt cttaaagttg ctattgcagg tgacttggac cactcacgcg
    13621 ttgccaaatc caatatgcag attttgaaac gcttgggatc tgaactcttt ttcgctggac
    13681 ctgaggaatg gagaagtcaa gagtttgcag actatggaca gtttgtaacc attgatgaaa
    13741 tcattgatca ggttgatgtc atgatgtttc tccgtgtgca acacgaacgc catgatagtg
    13801 gagctgtatt ttcaaaagaa gactaccatg cccaacatgg cttgactcaa gaacgttacg
    13861 atcgtttgaa agaaacagca atcctcatgc acccagctcc aatcaatcgt gatgtagaaa
    13921 tagcagacca cttggttgaa gcaccaaaat cacggattgt ccaacaaatg accaatggtg
    13981 tctttgttcg aatggcaatc ttagaatccg tactagcgag tagaaacgcc aactaaaaca
    14041 caagacgtta aaagacgcca gtgagcgtcc acgagaggtg aaaatatgac aaaaagaatt
    14101 ctagtattag aagatggcac agtttttgaa ggcaaggcct tcggagcaga tattgatgta
    14161 acaggcgaaa tcgtctttaa tacagggatg accggctacc aagaatccat tacagaccag
    14221 tcttataatg gacaaatctt gacctttact tatcctttgg taggaaatta tggtattaat
    14281 cgtgatgatt atgaatccat tattccaact tgtaagggag tcgttgtttt tgaagaagcg
    14341 cgtagagcta gcaactggcg caaccaaatg accttggatg aatttttgaa agccaagaaa
    14401 attccaggga tttcaggaat tgatacgcgt gatcttacca agattatccg taagcatggt
    14461 actatgcgtg caaccttgac ccatgttggg gacagtatgg accatgtgac ggaccagctc
    14521 caagcaacag ttttaccgac agacaatatc aaacaggttt ctactaaaac gtcttatcca
    14581 gctccaggag ttggtttgag cgtggtgcta gtggactttg gtctcaagca ctcaatccta
    14641 cgtgaacttt ctaaacgcaa ctgtaacgtg acagttgttc cttattcgac aacggctgaa
    14701 gaaattctcc atctcaatcc tgatggagtt atgttgtcaa atggtccagg taacccagaa
    14761 gacgttccac aagcactcga catgattcgc ggtgtacaag gaaaaattcc aatctttggt
    14821 atttgtatgg gacaccaact ctttgcaatg gcaaacgggg ctaagaccta taagatgaag
    14881 tttggccacc gtggattcaa ccatgcggta cgtgaaatcg caacaggacg agtagacttc
    14941 accagccaga accatggtta tgcagtcagc cgcgaggatt tgccagagca cttgattatt
    15001 acccacgaag aaatcaatga caagtcagtg gaaggtgtgc gccacagata ccaacctgct
    15061 ttctctgttc aataccaccc agatgcagct cctgggccac acgacgctag ctacctattt
    15121 gacgagttta tcgagatgat ggaagttttt aaacaatcaa actaagaacg agtaaaagct
    15181 cgtcggagaa tttatgtaac tgaacacgga cggaaagctc agaatgtaga gaaaccaact
    15241 tggattgtca atccttcctt gattttctaa aattcaatcg ctttctattc gtcctttgta
    15301 tcttatttaa tgtcgccctg agaggcgacg aatagaaagg agaagggtgc tccgtatttg
    15361 ctgaactgaa tacgggctac ggatggtgct aaaaagatag ttattcctag aactgacagt
    15421 tcttcgtcat aactcctatt ttagctttgt ccgcttgacg cccttaatat cttatatatg
    15481 cctaaacgta ctgatattca aaaaattatg gtgattggtt ctggtccgat tattattggt
    15541 caggctgctg agtttgacta cgctgggacc caggcttgct tgtcgttgaa agaggaaggt
    15601 tatgaggttg ttttggttaa ctcaaatcct gcaaccatca tgacggacaa ggaaattgct
    15661 gataaggttt acattgaacc gattacactt gagtttgtga cacgtattct tcgtaaggaa
    15721 cgtccagatg ccttgctacc aacactcggt ggtcagacag gtctcaacat ggccatggaa
    15781 ttgtctaaaa atggcatctt agatgagctt ggtgtagagc ttctcggtac tagattgtct
    15841 gccattgacc aagcggagga ccgtgacctc tttaaacaat tgatggaaga gcttgagcaa
    15901 cccattccag aatctgaaat tgttaacaca gtagaagaag caattgcctt tgcagcgaca
    15961 attggctacc cagtcatcgt tcgtccagcc tttacccttg gtggtactgg tggtggtatg
    16021 tgtgccaacg agaaagaatt gcgtgaaatc actgaaaatg gtttgaaatt gtcacctgtt
    16081 acccaatgtt tgattgagcg ttcgattgcc ggtttcaagg aaattgaata cgaagtgatg
    16141 cgtgactcag ctgacaatgc cttggttgtt tgtaacatgg aaaactttga cccagttggg
    16201 attcacacag gggattccat tgtatttgcc cctgcgcaaa ccatgtcaga ctatgaaaac
    16261 caaatgcttc gtgatgcgag cttgagcatt attcgtgccc tcaagattga aggtggatgt
    16321 aatgttcagc tagctcttga tcctaacagc ttcaagtatt atgttatcga agtaaaccct
    16381 cgtgtatcac gttcgtcagc ccttgcttct aaagcgacag gatatccgat tgctaagttg
    16441 gctgccaaga ttgcagtagg tttgaccttg gatgaggtta taaacccagt tacaggttca
    16501 acctatgcca tgtttgaacc agcccttgac tacgtagttg ctaagattcc acgtttccca
    16561 tttgacaagt ttgaaaaggg tgaacgccgt ctcggtacac aaatgaaggc gactggagaa
    16621 gtcatggcaa tcggtcgtaa catcgaggaa tctcttctca aggcctgccg ctcccttgaa
    16681 attggggtgc accacaatga aatacctgaa cttgcagcag tttctgatga tgccttgatt
    16741 gaaaaggttg tcaaagccca agatgaccgt ctcttctatg tgtcagaagc cattcgccgt
    16801 ggttacacac ctgaagaaat tgcagagctt acaaaaattg atatcttcta tcttgataaa
    16861 ctcttgcata tctttgaaat tgagcaagaa ttgggtgctc atccacaaga tctagaagtt
    16921 ttgaaaacag ccaaacttaa tggtttttca gaccgtaaga ttgctgaact ctggggaacg
    16981 actgatgatc aagttcgcca acttcgtttg gaaaacaaga ttgtcccagt ttataagatg
    17041 gtagacactt gtgcggcaga gttcgactct gaaacgccat atttctattc aacctatggt
    17101 tgggaaaatg aatctatcag atctgataaa gaatctgtac ttgtcctagg ttcaggtcca
    17161 atccgtatcg gtcaaggggt tgagtttgac tacgcaaccg ttcactctgt taaggctatc
    17221 caggcagctg gttatgaagc tattatcatg aactcaaacc cagagaccgt ttcaacagac
    17281 ttctctgtat cagataagct ttactttgag ccattgacat tcgaagatgt tatgaacgtc
    17341 attgacttgg aacaaccaaa gggagttatc gttcagttcg gtggtcaaac agccatcaac
    17401 cttgcggagc cattggcaaa agcaggtgtg accatccttg gtacacaggt tgctgaccta
    17461 gaccgtgccg aagaccgtga cctcttcgag caagctctca aagaattgga tattccacag
    17521 ccaccaggac aaacggctac caatgaagaa gaagcagctc ttgcagctcg taagattggc
    17581 ttcccagttc ttgtccgccc atcttatgta cttggtggac gtgccatgga aatcgttgaa
    17641 aacgaagaag accttcgttc ttacatgcga actgctgtta aggctagtcc agaccaccca
    17701 gttcttgtcg actcttacat cgttgggcaa gagtgcgaag ttgatgccat ttcagatggt
    17761 aaaaatgtac tcattcctgg tattatggag catatcgaac gtgctggtgt ccactcaggt
    17821 gactcaatgg ctgtttaccc accacaaacc ttgtcgcaaa aggttcagga gaccatcgca
    17881 gactacacca aacgcttagc aatcggtctt cactgccttg gaatgatgaa catccagttt
    17941 gttatcaagg atgaaaaagt ctacgttatt gaggtcaatc cacgtgccag ccgtacagtt
    18001 ccattcctat ctaaggtaac caatattcct atggctcaag tagcgaccaa gctcattctt
    18061 ggtcaaagtt tatcagaact tggctaccaa aatggacttt accctgaaag cacccgtgtt
    18121 catatcaagg cacctgtctt ctcctttacc aaactagcta aggtagacag cttgctcggt
    18181 cctgaaatga agtcaacagg tgaagttatg ggttctgatg ctactttgga aaaagctctc
    18241 tataaagcct ttgaagcttc ttatctacac ttgccaactt ttggtaatgt cgtctttacc
    18301 atcgctgatg atgccaaaga agaagccttg aacttggctc gtcgtttcca aaacattggt
    18361 tatggaatcc ttgcgacaga agggacagca gccttctttg ctagtcatgg cttgcaagcc
    18421 caacctgttg gtaagattgg tgatgatgat aaggatatcc caagttttgt acgcaaagga
    18481 agaattcaag ctatcattaa cacagtagga acaaaacgaa ctgctgacga agatggtgag
    18541 caaattcgtc gttcagccat tgaacacggg gtgcccctct tcacagcgct agatacagct
    18601 aatgccatgc ttaaggtact tgaaagtcgt agctttgtta cagaagcgat ttaaatagaa
    18661 aaataa
//
LOCUS       DAVLTM010000027        18536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLTM010000027 DAVLTM010000000
VERSION     DAVLTM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.027"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            435..1145
                     /gene="deoD"
                     /locus_tag="VYN16_000845"
     CDS             435..1145
                     /gene="deoD"
                     /locus_tag="VYN16_000845"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1403402.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYACELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1168..1692)
                     /locus_tag="VYN16_000846"
     CDS             complement(1168..1692)
                     /locus_tag="VYN16_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1403403.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1745..1981)
                     /locus_tag="VYN16_000847"
     CDS             complement(1745..1981)
                     /locus_tag="VYN16_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1403404.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2049..2969)
                     /locus_tag="VYN16_000848"
     CDS             complement(2049..2969)
                     /locus_tag="VYN16_000848"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1403405.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3159..3526
                     /locus_tag="VYN16_000849"
                     /pseudo
     CDS             3159..3526
                     /locus_tag="VYN16_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3742..4323
                     /locus_tag="VYN16_000850"
     CDS             3742..4323
                     /locus_tag="VYN16_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1403406.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLYVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4320..5597
                     /locus_tag="VYN16_000851"
     CDS             4320..5597
                     /locus_tag="VYN16_000851"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1403407.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5615..6277
                     /gene="deoC"
                     /locus_tag="VYN16_000852"
     CDS             5615..6277
                     /gene="deoC"
                     /locus_tag="VYN16_000852"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1403408.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGSDFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6264..6653
                     /locus_tag="VYN16_000853"
     CDS             6264..6653
                     /locus_tag="VYN16_000853"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1403409.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDRSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6743..7795
                     /locus_tag="VYN16_000854"
     CDS             6743..7795
                     /locus_tag="VYN16_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1403410.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7939..9474
                     /locus_tag="VYN16_000855"
     CDS             7939..9474
                     /locus_tag="VYN16_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403411.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9467..10525
                     /locus_tag="VYN16_000856"
     CDS             9467..10525
                     /locus_tag="VYN16_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403412.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10528..11484
                     /locus_tag="VYN16_000857"
     CDS             10528..11484
                     /locus_tag="VYN16_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403413.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            11569..12876
                     /locus_tag="VYN16_000858"
                     /pseudo
     CDS             11569..12876
                     /locus_tag="VYN16_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12919..13560)
                     /gene="plsY"
                     /locus_tag="VYN16_000859"
     CDS             complement(12919..13560)
                     /gene="plsY"
                     /locus_tag="VYN16_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1403414.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            13696..15639
                     /gene="parE"
                     /locus_tag="VYN16_000860"
     CDS             13696..15639
                     /gene="parE"
                     /locus_tag="VYN16_000860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1403415.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            16059..18530
                     /gene="parC"
                     /locus_tag="VYN16_000861"
     CDS             16059..18530
                     /gene="parC"
                     /locus_tag="VYN16_000861"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1403416.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQTEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
BASE COUNT         5516 a         3411 c         4211 g         5398 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaaccaccc
      181 taaaagcagt gggaaaaaga tagttggtct agcgagcatc gctcactgcg cccaactcct
      241 attttccctt cgctttttga cgggtttggt atctttctca atataaaata taaaataaag
      301 aaaggtagag cgtgtgtttt gatttgaaca cgagcggaaa actcggaaaa tagataatct
      361 gactgaaaaa tcaggatttc tcgtcaggtt cctaattttc agtcgttttc ttctcgctct
      421 ttgtatcata aattatgtct atccatattg ctgctcagca gggtgaaatt gctgataaaa
      481 ttcttcttcc tggggatcct cttcgtgcta agtttattgc ggagaatttc cttggtgatg
      541 ctgtttgttt taacgaagtg cgtaacatgt ttggttacac tggtacttac aagggtcacc
      601 gtgtatctgt catgggaact gggatgggaa tgccatctat ttcgatttat gcgtgtgagt
      661 taatcgtaga ctacggtgtg aagaaattga ttcgtgtggg aactgcaggt tctttgaatg
      721 aagaggttca tgttcgtgaa ttagttttgg cgcaggcggc tgcaaccaac tcaaacatcg
      781 ttcgtaatga ctggccacag tacgattttc cacaaattgc tagctttgat ttgcttgata
      841 aagcctacca tatcgccaaa aaacttggta tgactactca cgttgggaac gttttgtcat
      901 ctgatgtctt ttactcaaat tactttgaaa agaatatcga gcttggtaaa tggggagtca
      961 aggctgtgga aatggaagca gcagctcttt actatcttgc tgcccaatac catgttgatg
     1021 cgctagctat catgaccatc tctgatagct tggtcaatcc agacgaagac acaactgcag
     1081 aagaacgtca aaataccttc actgatatga tgaaggttgg tttggaaacc ttgattgcag
     1141 aataattata gccaaaaagg aagcccttta tgaattgtag gacttccttt tcttatccag
     1201 aaattgatct agctctctct gatttcgaag aatagtgact ttatgtgaat attcttggca
     1261 aagtttttgg taattttctt tttgagtttt gctacgccca tcccaaagaa tccatctgat
     1321 aaactcccac tcaaagcgtt cagggcaatc tgccgccata ctttctctga cttttccacg
     1381 gtatttaaga taacgcttaa aggctctaaa gagacaggtc aatggcgaaa aattgagaaa
     1441 gatgatttgg tcagcttctt gcattcgttc ttggtagtag caccaagaat aattaccatc
     1501 gatgacccaa gctttatgct tggtgagaaa gttttttatc tcggttaaca tccattcgca
     1561 gtcactgtct tgccaaccag gttgaaattg gagtgtgtcc atgtgcagtt ttggaatgga
     1621 gtagtagtta gataactttt ctgctagagt tgacttacca gaaccagaat atccgataat
     1681 tgcgattttc attttctacc ttttcctatt tggagacaaa aaaacagcct ctatggactg
     1741 tttcttattt agcaagttta gctgaaagac gagctttatc gcggcttgct ttgtttttgt
     1801 gaatcaaacc tttagtttct gctttatcga tagctgagct agcagcacgg aaaagttctt
     1861 cagatgggtt tgcttcgaaa gcttttatag cagtacgcat agctgatttt tgagctgagt
     1921 tcttttcgtt ttgtttaacg ttcaattcag cgcgtttgat agctgattta atgtttgcca
     1981 atgttcttac ctccatattt actaactata ccattatata tgaaaactta cgttttgaca
     2041 agggaaaatt acttttttaa gtaaatttca tcgatttcat ggttcttgct tttatgaaga
     2101 atcacttctg cacgatttct ggttggttca atataatttt gcagatttgt gagattgata
     2161 ctggtccaga cctgatgggc aaaggactcc acttccccaa tcggcatctg agtaaaacga
     2221 taatagtagc tatcagggtc gttttgggct aggctcagca ttttcaagaa acggtccaga
     2281 taccaacttt caatatcatc cactccagca tctacataga tggaaaagtc aaagaagtca
     2341 gtgatataga gacgatcgtt ttgtggattt tgaaagacat taattccctc aacaattaca
     2401 aaatcagcag ctttgacact ttgtttcttt tcgggtacga tgtcgtaaac ttcatgagaa
     2461 tagacaggaa tatctacatc ttgtccattt ttgatgcggt ccaagaagtt gagaagagct
     2521 tccatatcat agctttcagg aaatccttta cgatttaaaa tcccctgctc aatcaaggtt
     2581 tgattgggat agagaaaacc atcagttgta accaactcaa ccgtagcatc tgtaaacgta
     2641 cgggacagta ggatttgaag taggcgactg gttgtggatt ttccaacggc aacactccca
     2701 gaaaccccaa taataaaagg ttgagattta ctttcacgtt ggaggaaaat tccttttgaa
     2761 aaggctaaat cttccttagt tcgcttgtaa atctgaatca gatgagccaa ggggagatag
     2821 atatctgtaa cgtcttggag actgatttgg tcattaaaac tcttgataga ttccaattct
     2881 tcttctgtca aaggaggtgt tgtctttcga tgtaaagatt gccaagtctg gcggctgatt
     2941 ttttcaaaat gtaaaaattc gttggtcatt tgttttcccc taggtatttt atctatcata
     3001 ccataaaaag ctaaaaaaat aaagcggttt cttgaatgtt gtaagtcaat gatatataat
     3061 ataagcgatc aatttattga tgaaatagga gatagattct actgatttat aagcttataa
     3121 agggggcttt ttagttcaca acttatagga gatgtactat gaaaatctta aaacgttaca
     3181 tattggaact ctgttttatt ttaagttttg ctttaccttt tataaaagga gccaatgcag
     3241 ataatggtag atgctttgtg gaaacctatt acggttttac ttttttgatg gaacatgcta
     3301 ttgtaacagc tgtctttatc tgttcgttct taattgctgt cttactaaaa aacgatgggc
     3361 gaaatggatt gcgacagagg gttatttctt ccgcatgtca ctagaagatt tgatacgact
     3421 ttggacaagt ttggaaatcc tgacacaaac gtatcagttg ggcttttatc taaacatttt
     3481 gttaggaatt ttgttgataa taaagtattt taaggttaag caatagaaat aataatctgc
     3541 ctgattatag atgataaact ttttgttttt atctggactg tgatatttat agttctaaat
     3601 ggtttattat ttataagtgt tgaaagagat tgaagaaaat ggcttcgtga cttgctttct
     3661 tcggtctttt tcgttttatt atcaatttgt aaacgattta caatttgagt caaattatgg
     3721 taaaatagtg tcatgagtaa aatgtattat gcagaaaatc ctgacgctgc tcacgacatt
     3781 catgagttga gagtggactt gttgggacaa aaaatgactt ttttgacaga tgcgggtgtt
     3841 tttagcaaga aaatggttga ctttggaagt caactcttgc tcaagtgtct gtatgtcaac
     3901 caaggagaga cagtcctaga tgtaggctgt ggttatggtc cattaggttt gtccttggtc
     3961 aaggtttacg gagttcaggc gaccatggtt gatattaaca ctcgtgcctt ggatttagct
     4021 cggagaaatg ctgaaaaaaa taatgcaaaa gcgacgattt tccagtccaa catctatgaa
     4081 caagttgaag gacattttga tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa
     4141 gtcgttcatg aaatcattga gaaaagtaaa gatttcttgg aaactggtgg ggatttgaca
     4201 atcgtcatcc agaaaaaaca aggggctcca agtgctaagt ccaagatgga ggatgtgttt
     4261 ggtaattgtg aaatccttaa aaaagacaaa ggatactata tccttagaag tgtgaaagaa
     4321 tgagagcagt tgatttaata caaaaaaaac gagacggtca agaactgact tcaagtgaaa
     4381 ttgaatggct ggtagaaggc tatgtgtcag gaactgttcc tgattatcag atgtctgcct
     4441 ttgctatggc tgtttatttt aaaggaatga cgactcgtga aatttctgat ttaacgatga
     4501 atatggttaa gactgggcaa gagtttgacc tgtcagccat tgatggtgtg aaagttgaca
     4561 agcattctac tggtggtgtc ggcgataagg taaccttgat tttagctcct cttgttgcta
     4621 gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct tggccataca ggcggaacaa
     4681 ttgataaatt ggagtccatt aagggctatc aagtagaacg tagtcaagag gattttattc
     4741 gtcaggttca ggatattgga gtatctgtca ttgggcaatc agaccagctg gtcaaagcag
     4801 ataagcttct ctatgccctt cgtgatgtga ccgcaactgt cgacacgatt cctttgattg
     4861 cgagttcggt gatgagcaag aaaattgcgg caggggcaga tgctattttg ctagacgtga
     4921 ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta
     4981 tggtagatct tggtaaggtt gttggacgga agacagtagc agtgattacg gatatgagcc
     5041 agcctttggg acgagcgatt ggaaatcgtc tggaaatcct tgaagcattg gagattttgc
     5101 aaggtcaagg acgtcaggat attacccact ttatttgtga attggctcaa attatgcttg
     5161 gtttggcaaa tgttaacaaa actgttgaag aagttcgcca acatcttgag aatggtcaag
     5221 cactggctaa gtttgaggaa atggtgcaag cccaaggtgg agacttggaa gacctctatc
     5281 gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca ggaaacgggt gtcatttcag
     5341 ctcttccagc tatggacttt ggtctttatg ccatgaggct gggtgctggt cgtgcagtta
     5401 agtctgatgc cttggactat gaaacaggaa ttgtttttga aaagaagatt ggagactccg
     5461 ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga aaaaattcca cctcaactag
     5521 ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg
     5581 tagaaatcat ctcctaaatg gagggaaaac cgaaatgaaa ttaaataaat atatagatca
     5641 tacgctttta aaacaagatg caaagaaaaa acaaattgat agtttgttgt ctgaggctag
     5701 agagtatggc tttgccagtg tttgcgttaa tccgacctgg gttgaacatg ctaaaaaagg
     5761 acttgaaggc acagatgtta aggtttgcac agtagtaggt ttccccttgg gagcaacaac
     5821 ttcaactgtg aaagcttttg aaacaaaaga agctatccaa aatggggctg atgagattga
     5881 catggtaatc aatgttgggg ctctcaagtc aggtgatttg gccttggttg agtcggatat
     5941 tcgtgcagta gtggaagcaa gtggggacaa gttagtgaaa gtcattattg aagcttgcct
     6001 tctgacagac caagaaaaag ttttggcttg ccaattagcc caaaaagcag gatctgactt
     6061 tgtcaaaaca tctactggct tttcaactgg tggtgctacg atagcagatg ttagattaat
     6121 gcgtgaaaca gttggacctg atatgggtgt caaggccgcc ggtggagctc gttcttatgc
     6181 agatgctctt acctttgtcg aagcaggtgc gacccgtatc ggaacgtcag ctggggtagc
     6241 tattttaaaa ggagaattgg cagatggcga ctactgagtt gattgaactg gcaattgaaa
     6301 ccagcaaaca tgcctatgtc ccctattctc actttcctat cggagcagtt ttagtagcca
     6361 aagaccgaag tgtttacacg ggagtgaata tcgaaaatgc tagctatcct ttgaccaatt
     6421 gcggtgagag aacagctatt tttaaggcta tatctgaagg ccaaagagaa ttttcagaat
     6481 tgattgtcta tggtcaaact gaaaaaccga tttcaccatg tggtgcttgt cgccaagtaa
     6541 tggtcgaatt ttttgaacaa gatttaaaag tgaccttagt cgcaaaagat aaatcgacgg
     6601 tcgagatgac ggtcggggaa ttacttccat actcttttac agacttaaac tagtctgagt
     6661 cgctctctga gtggcacggt ccttgtggcc aaccaatcca tacttgcaac atcgttgcac
     6721 atcttattta ggaggttcag taatgaacaa gaaacaatgg ctaggccttg gtctagttgc
     6781 agtggcagca gttggacttg ctgcatgtgg taaccgctct tctcgtaacg cagcttcatc
     6841 ttctgatgtg aagacaaaag cagcaatcgt cactgatact ggtggtgttg atgacaaatc
     6901 attcaaccaa tcagcttggg aaggtttgca ggcttggggt aaagaacaca atctttcaaa
     6961 agataacggt ttcacttact tccaatcaac aagtgaagct gactacgcta acaacttgca
     7021 acaagcggct ggaagttaca acctaatctt tggtgttggt tttgcccttc acaatgcagt
     7081 tgaagaagca gctaaagaac acactgactt gaactatgtc ttgattgatg atgtgattaa
     7141 agaccaaaag aatgttgcga gcgtaacttt cgctgataat gagtcaggtt accttgcagg
     7201 tgtggctgca gcaaaaacaa ctaagacaaa acaagttggt tttgtaggtg gtatcgaatc
     7261 tgaagttatc tctcgttttg aagcaggatt caaggctggt gttgcgtcag tagacccatc
     7321 tatcaaagtc caagttgact acgctggttc atttggtgat gcggctaaag gtaaaacaat
     7381 tgcagccgca caatacgcag ccggtgcaga tattgtttac caagtagctg gtggtacagg
     7441 tgcaggtgtc tttgcagagg caaaatctct caacgaaagc cgtcctgaaa atgaaaaagt
     7501 ttgggttatc ggtgttgatc gtgaccaaga agcagaaggt aaatacactt ctaaagatgg
     7561 caaagaatca aactttgttc ttgtatctac tttgaaacaa gttggtacaa ctgtaaaaga
     7621 tatttctaac aaggcagaaa aaggagaatt ccctggcggt caagtgatcg tttactcatt
     7681 gaaggataaa ggggttgact tggcagtaac aaacctttca gaagaaggta aaaaagctgt
     7741 cgaagatgca aaagctaaaa tccttgatgg aagcgtaaaa gttcctgaaa aataatggat
     7801 ggaagtcatt tcttaggaag cggccaaggg ccgctttttt aagagttgag acaaagtcat
     7861 tttgtctcag taaagcttgc tactagacaa actagcaagt tttattgaaa taaaataaga
     7921 ctctgaaagg aagagcacat ggcacacgaa aatgtcattg agatgcgtga tattaccaag
     7981 gtgtttggtg gatttgttgc caacgacaaa atcaacttgc acctacgaaa aggtgaaatt
     8041 catgcacttt taggagaaaa tggggctggt aagtccacgc taatgaacat gttagcaggc
     8101 cttcttgaac caactagtgg tgaaatcgcg gtcaacggtc aagttgtcaa tctcgactcc
     8161 ccatctaaag cagctagctt gggaatcggg atggttcacc agcactttat gttggttgaa
     8221 gccttcacag tggctgaaaa catcatttta ggtagtgaat tgactaaaaa tggtgtgcta
     8281 gatatcgctg gagctagcaa agaaatcaag gctctttctg aacgttatgg cttagctgtt
     8341 gacccttctg ccaaggtagc agatatctca gttggagccc aacaacgtgt agaaatttta
     8401 aaaacacttt atcggggggc tgatatcctt atctttgacg aaccaacggc tgttttgact
     8461 ccatcagaaa ttgatgagtt gatggctatt atgaaaaatc ttgtcaaaga aggaaaatca
     8521 attatcttga ttacccacaa attggatgaa attcgcgcag tttctgaccg tgttacagtt
     8581 atccgtcgtg ggaaatcaat tgaaaccgtt gaaattgcag gggctaccaa tgctgatttg
     8641 gcggaaatga tggtaggacg ttctgtttcc tttaaaacag agaagcaagc ctctaaacca
     8701 aaagaagtgg ttttgtctat caaagatttg gtggtcaatg aaaaccgtgg tgttccagct
     8761 gttaaaaatc tgtccttgga tgttcgtgct ggagagattg ttggtattgc ggggattgat
     8821 ggaaatggtc agtctgaact gattcaagcc attacaggtc ttcgtaaggt tgaatctggt
     8881 agcattgagc taaaaggaga ttcaattgta ggcttgcacc cacgtcagat tacagaacta
     8941 agtgttgggc acgttccaga agaccgtcac cgtgatggct tgattttgga aatgatgata
     9001 tctgaaaata ttgcccttca aacctactat aaagaaccac atagtaaaaa tggaattttg
     9061 aattattcaa atattacttc ttatgctaaa aagctgatgg aagagtttga tgttcgcgct
     9121 gccagtgaat tagttcctgc agctgcactc tcaggaggaa atcaacaaaa agcaattatt
     9181 gctcgtgaaa ttgatcgaga tcctgatctc cttatcgtta gccagccaac tcgtgggttg
     9241 gatgtcggtg ccattgagta tatccacaaa cgcttgattg aagagcgtga taatggcaag
     9301 gctgtccttg ttgtcagctt tgaattggat gagattttaa acgtctcaga ccgtattgcc
     9361 gttatccacg atggtaagat tcaaggtatt gtatcaccag aaacaaccaa taaacaagaa
     9421 cttggtgtct tgatggctgg tggaaacttg ggaaaggaga agagtgatgt ctaaaaaatt
     9481 acaacaaatt tcggttccct tgatttctgt atttctagga attttactcg gagccattgt
     9541 catgtggatc ttcggttatg atgctatttg gggctacgaa gaattgttct atacagcctt
     9601 tggcagtctg cgtgggattg gagaaatctt ccgtgctatg ggtcctctgg tcttgattgg
     9661 tcttggtttt gcggttgcca gtcgagctgg tttctttaac gtcggacttc ctggtcaggc
     9721 tttggcaggt tggattctca gtggttggtt tgcgctgtcg catccagata tgccccgtcc
     9781 cttgatgatt ctagcaacca tcgtgattgc cttgattgct ggtgggattg tcggagcgat
     9841 tccaggtatt cttagggcct atctagggac gtcagaggtt attgtaacca tcatgatgaa
     9901 ctacattgtc ttgtatgtag ggaatgcctt tatccatgct ttccctaaag acttcatgca
     9961 aagtacagat tcgaccattc gtgttggggc taatgcaacc tatcagacac cttggttggc
    10021 tgagttgact ggtaactcac ggatgaatat tggtattttc tttgccatca ttgccgttgc
    10081 agttatttgg ttcatgctca agaaaacaac tcttggtttt gaaatccgtg cagttggtct
    10141 taatccacat gcttcagaat atgctggtat ttctgccaag cggactatta tcctatctat
    10201 gattatttca ggtgccttgg caggtcttgg tggagctgtt gaaggtttgg gaaccttcca
    10261 gaacgtctat gttcaaggtt cgtcattagc tatcggattt aacggaatgg cggttagttt
    10321 gcttgcggcc aactcaccaa ttggtatact ttttgcagcc ttcctatttg gcgttctcca
    10381 agttggggct cctggtatga atgcggcgca ggtaccatct gagcttgtca gcattgtaac
    10441 agcgtctatt atcttctttg tcagtgttca ttaccttatc gaacgctttg tcaaaccgaa
    10501 aaaacaagtt aaaggaggta agtaaagatg tctattataa ccttgctccc cctcttggtg
    10561 tcttctatgc tgatttactc agcacctctc atctttacaa gtatcggtgg tgttttctct
    10621 gaacgtggtg gtgtggtaaa cgttggcctt gaaggaatta tggttatggg tgccttttct
    10681 ggagttgtct ttaaccttga atttgcagaa caatttggag cagcaactcc atggctatcc
    10741 ttgcttgtag caggattggt tggtagtgtt ttctctatca tccacgcagc agcgacggtt
    10801 catttccgtg cagaccatgt tgtcagcggt acggtattga acttgatggc gcctgccttg
    10861 gctgttttct tagttaaagt tctctataac aaaggacaaa ccgacaacct aagtcaaact
    10921 tttggacgct ttgatttccc agtcttggca aatatcccag tgattggtga tatcttcttc
    10981 aagtcaacta gtctacttgg ttatctggcg attgccttct cattccttgc ttggtttatt
    11041 ctctttaaga ctcaatttgg tcttcgtctc cgctctgtcg gtgaacatcc tcaagcagcg
    11101 gataccctgg gaatcaacgt ctacaagatg agatatttag gggttattat ttcaggtttt
    11161 ctaggtggaa ttggcggagc gatttatgcg caatcaatct cagttaactt ctcagtgaca
    11221 actattgttg gacctggatt tatcgccctt gctgcgatga tctttgggaa atggaatcca
    11281 atcggtgcta tgctttctag tctcttcttt ggactttcac aaagtttggc tgttatcggt
    11341 tctcaattgc cgttcctaca aggagtgcca gcggtttatc ttcaaattgc accttatgtt
    11401 ttgacaattc ttgtcttagc agccttcttt ggaaaagcag tcgcaccaaa agcagatggt
    11461 atcaactaca tcaaatcaaa ataagcatac aaaaaaacgt cagtccaatg actgaactgc
    11521 accccaaaag ttagacagaa aaaatctaac ttttggggtg tttttattat gaaattgagt
    11581 tgtgatgata aagttcagat ctatgaactt agaaaacaag gatatagctt agagaagctt
    11641 tcaaataaat ttgggataaa caattctaat cttaggtaca tgattaaatt gattgatcgt
    11701 tacggaatag agttcgtcaa aaaagggaaa aatcgttact attctcctga tttaaaacaa
    11761 gaaatgattc ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac
    11821 tgtctcccaa gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat
    11881 actattgttg agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa
    11941 gttaagagaa ctccgattga aggaggaaaa agagaaataa gaaagacaga aattgttcaa
    12001 gaattaatga ctgagttttc gttagatctt cttctaaaat ccattaaact agctcgttcg
    12061 acctactact atcacttgaa acagctagac aaaacagata aggaccaaga gcttaaagct
    12121 gaaattcaat ccatctttat cgaacacaag ggaaattatg cttatcgtcg gatttattta
    12181 gaactaagaa atcgtggtta tctggtaaat cataaaagag ttcaacactt gatgaagtac
    12241 tcagtttaca agctaaaacg cgacagaaac gaaaatattc ttctcataaa ggagacgttg
    12301 gcaagaaggc agagaatctc attcaaggcc aatttgaagg ctctaaaaca atggaacagt
    12361 gctacacaga tgtgactgaa tttgccattc cagtaagtac tcaaaagctt tacttatcac
    12421 cagttttaga tggctttaac agcgaaatta tcgcctataa tctttcttgt tcgcctaatt
    12481 tagaacaagt acaaacaatg ttggaacagg cattcaaaga gaagcactat gagaatacga
    12541 ttctccatag tgaccaaggc tgtcaatacc aacacgattc tccatagtga ccaaggctgt
    12601 caataccaac acgattctta tcatcggttc ctagagagta agggaattca agcatccatg
    12661 tcacgcaagg gaaacagcca agacaacggt atgatggaat ctttctttgg cattttaaaa
    12721 tccgaaatgt tttatggcta tgagaaaaca tttaaatcac ttaatcaatt ggaacaagcc
    12781 attatagact atattgatta ttacaacaac aagagaatta aggtaaaact aaaaggactt
    12841 agccctgtgc aatacagaac taaatccttc ggataaatta attgtctaac tgtttggggg
    12901 cagtacagaa ctggcgtttt attttttagg atcttgatga gttaggttca atccccaagg
    12961 gaccaaattt tcagttttat ttttgatacg agctatattg tccttatgac gaatgataat
    13021 caaactagca agtgctaaga taatagcgat gaagagagag tcatagttac tcaggataaa
    13081 accaaaaagt ggaaagagca gaaccccgat aacagccgcg atcgatgctg tgacactaga
    13141 cagtgaaatc atactgccaa gatagagagc tccaaagaag ataatcgcaa ggtagagaca
    13201 gaagataggc gcaaatccga aaatcactcc agcactggtt gcgacagcct taccaccttt
    13261 aaatcctgca aagataggga aggtatggcc gataacagcc aaaagtccaa agatgagagg
    13321 agaaacgcct tgtagatgaa aaataatcgg aagcagcgtt gctagggttc ctttgaaaaa
    13381 gtcaatcaca aaggttgcca taccagcttt cttacctaaa atgcggaagg tattggtcgt
    13441 tccagtgtta ccagaaccat gctcgcgtag attgatttga aagaatactt gtccaatcca
    13501 gagaccagat ggaatcgaac ccagcagata ggctaggatt aataaaacta ttgtaatcat
    13561 actcctatta tatcatgaat tgggaaagaa aagcagagaa tctctgctga aattgtcaca
    13621 tcggagaaag aaaaattttg caaaatcctt ggaaaacctg tagaatagta aagatgaacg
    13681 aataggaggt tccttgtgtc aaaaaaggaa atcaatatta acaattataa tgatgatgct
    13741 attcaggtgc tagaagggtt ggatgcggtc cgaaaacgtc cggggatgta tattggatcg
    13801 accgatggcg ctggtcttca tcacctagtt tgggaaatcg ttgataatgc agtcgatgaa
    13861 gccttgtctg ggtttggtga tcgtatcgat gtaactatca ataaagacgg tagtctaacg
    13921 gttcaagacc atggacgtgg gatgccgaca ggtatgcacg ctatgggaat tccaactgtt
    13981 gaggttatct ttaccattct tcatgccgga gggaaattcg gtcaaggtgg ctataagaca
    14041 tcaggtggac ttcacggagt gggttcttcc gttgttaacg ccctttctag ctggttagaa
    14101 gttgaaatta cccgtgatgg cgcagtttac aagcaacgtt tcgaaaatgg tggaaaacct
    14161 gtcacgactt tgaagaaaat cggtacagca cccaagtcta aaacaggcac caaagttact
    14221 tttatgcctg acgcgactat cttttctacg acagatttca agtacaatac catttcagag
    14281 cgccttaatg aatcagcctt tctcttgaaa aatgtgacct tgtctttaac ggacaagcga
    14341 acagatgaag cgattgagtt ccactatgag aatggagtac aagattttgt ttcttatctc
    14401 aacgaagata aggaaatctt gacgccagtt ctttactttg aaggggaaga caatggtttt
    14461 caagtggaag tagccctcca gtacaatgac ggattctcag ataacattct atcctttgtc
    14521 aataacgttc gcaccaagga cggtggaacg cacgagacag gactcaagtc tgccattacc
    14581 aaggtcatga atgactatgc acgtaaaaca ggtcttctca aggaaaaaga taaaaacctt
    14641 gaaggttcag actatcgtga gggactagcg gccgttcttt ctatcttagt tcctgaagaa
    14701 cacttgcagt ttgaaggaca gaccaaggat aaactaggaa gccccctagc tcgcccagtt
    14761 gtggatggaa tagtggctga taagttgacc tttttcctta tggaaaatgg ggaattagct
    14821 tctaacctca tccgcaaggc tatcaaggcc cgtgatgctc gtgaagcagc acgtaaggcg
    14881 cgtgatgaga gccgaaatgg gaagaaaaac aagaaagata agggcttgtt gtctgggaaa
    14941 ttgaccccag cccaatctaa gaatcctgct aagaatgaac tctatctagt tgagggggac
    15001 tctgccggtg gttctgccaa acaaggtcgt gaccgcaagt tccaggctat tctacctctt
    15061 cgtggtaagg ttgtcaatac agccaaggcc aagatggcgg atatcctcaa aaatgaagag
    15121 atcaatacca tgatttatac cattggtgcg ggtgttggag cagacttctc tattgaagat
    15181 gccaactatg ataagatcat tatcatgacc gatgcggata ccgacggtgc ccatatccag
    15241 accttgctct tgacattttt ctaccgttac atgcgtccgc tagtcgaggc aggtcatgtc
    15301 tatattgccc tcccacctct ttacaagatg tccaaaggta aaggcaagaa agaagaagtg
    15361 gcctacgctt ggacggacgg agaactagaa gaactccgta aacagttcgg taaaggcgct
    15421 accctccaac gatacaaagg acttggtgag atgaatgcgg accagctctg ggaaacaacc
    15481 atgaacccag aaacacgtac cctcatccgt gtcacaattg aagatttagc gcgcgccgaa
    15541 cgccgcgtca atgttctcat gggagataag gtagaaccac gccgtaaatg gattgaagat
    15601 aatgtcaagt ttacgctaga agaagcgaca gtgttttaat gaaagaaaat aatatgttag
    15661 taaactggaa attagagtcg gatgtgaatg actatgtaaa aaagcagttt gaaaatcttg
    15721 gtttgaaaaa attgcaggat tattaatgaa gaatcttcta tgagtgctta tctcaaagag
    15781 gcgtttaagg gagcggctaa gacacaaact aagactaatt ttggaaaacc agattttcat
    15841 attgaaaaat acaaacagga tagtttggtt acttatcgta actgaacacg ggacgaattc
    15901 ccgtaaaata tcttttactg cataaaaacc tactctacat tctttgaaag gagttgaaca
    15961 cgccctagat actgtgtgaa aaagataaat tctcttgtga gtttgcttac ttcaagaatt
    16021 ttctattttc acttggtatt ttatgggctt tgtatcttat gtctaacatt caaaacatgt
    16081 ccctggagga catcatggga gagcgctttg gtcgctactc caagtacatt attcaagacc
    16141 gggctttgcc agatattcgt gatgggttga agccggttca gcgccgtatt ctttattcta
    16201 tgaataagga tagcaatact tttgacaaga gctaccgtaa gtcggccaag tcagtcggga
    16261 acatcatggg gaatttccac ccacacgggg attcttctat ctatgatgcc atggttcgta
    16321 tgtcacagaa ctggaaaaat cgtgagattc tagttgaaat gcacggtaat aacggttcta
    16381 tggacggaga tcctcctgcg gctatgcgtt atactgaggc acgtttgtct gaaattgcag
    16441 gctaccttct tcaggatatc gagaaaaaga cagttccttt tgcatggaac tttgacgata
    16501 cggagaaaga accaacggtc ttgccagcag cctttccaaa cctcttggtc aatggttcga
    16561 ctgggatttc ggctggttat gccacagaca ttcctcccca taatttagct gaggtcatag
    16621 atgctgcagt ttacatgatt gaccacccaa ctgcaaagat tgataaactc atggaattct
    16681 tgcctggacc agacttccct acaggggcta ttattcaggg tcgtgatgaa atcaagaaag
    16741 cttatgagac tgggaaaggg cgcgtggttg ttcgttccaa gactgaaatt gaaaagctaa
    16801 aaggtggtaa ggaacaaatc gttattactg agattcctta tgaaatcaat aaggccaatc
    16861 tagtcaagaa aatcgatgat gttcgtgtta ataacaaggt agctgggatt gctgaggttc
    16921 gtgatgagtc tgaccgtgat ggtcttcgta tcgctatcga acttaagaaa gacgctaata
    16981 ctgagcttgt tctcaactac ttatttaagt acaccgacct acaaatcaac tacaacttta
    17041 atatggtggc gattgacaat ttcacacctc gtcaggttgg gattgttcca atcctgtcta
    17101 gctacatcgc tcaccgtcga gaagtgattt tggcgcgttc acgctttgac aaagaaaagg
    17161 ctgagaaacg tctccatatc gtcgaaggtt tgattcgtgt gatttcgatt ttggatgaag
    17221 tcattgctct tatccgtgct tctgagaata aggcggacgc caaggaaaac ctcaaagtta
    17281 gctatgattt tacggaagaa cagactgagg ctatcgtaac tttgcaactg taccgtttga
    17341 ccaataccga tgtggttgtc ttgcaggaag aagaagcaga gcttcgtgag aagattgcta
    17401 tgctggcggc tattatcggt gatgaaagga ctatgtacaa tctcatgaag aaagaacttc
    17461 gtgaggtcaa gaagaaattt gcaactcctc gtttgagttc tttagaagac actgcgaaag
    17521 caattgagat tgatacagct agtcttatcg ctgaggaaga tacctacgtc agcgtgacca
    17581 aggcaggtta catcaagcgt accagtccac gttcctttgc ggcttccacc ttggaagaaa
    17641 ttggcaagcg tgatgatgac cgtttgattt ttgttcaatc tgccaagaca acccagcacc
    17701 tcttgatgtt cacaagtctt ggaaatgtca tctacagacc aatccatgag ttggcagata
    17761 ttcgttggaa ggacatcgga gagcatctga gccaaaccat cacaaacttt gaaacgaatg
    17821 aagaaatcct ttatgtggaa gtactggatc agtttgacga tgcgacaacc tactttgcag
    17881 tgactcgcct tggtcaaatc aaacgggtag agcgaaaaga attcactcca tggcggacct
    17941 atagatctaa gtctgtcaag tatgctaagc tcaaagacga tacagatcag attgtagcag
    18001 tggctccgat taaactagat gatgttgtct tggttagtca aaatggttat gccctgcgtt
    18061 tcaatatcga agaggttccg gttgtcggtg ctaaggcagc aggtgtcaag gctatgaatt
    18121 tgaaagaaga tgatgtcctc caatctggct ttatctgtaa tacttcgtcc ttctacctct
    18181 tgacccagcg tggaagcttg aaacgtgttt ccattgagga aattctagca accagccgtg
    18241 ccaaacgagg attacaagtc ttgcgtgagt tgaaaaacaa accgcatcgt gtcttcttgg
    18301 caggagcagt tgcagagcaa ggatttgttg gcgatttctt cagtacggaa gtggatgtga
    18361 acgaccaaac tctacttgtc caatccaata aaggaacaat ctatgaaagc cgattgcaag
    18421 acttgaactt gtcagaacgc actagcaatg gaagcttcat ttctgacacg atttcagatg
    18481 aagaagtttt tgacgcttat cttcaggaag tagttactga agataaataa gtgaaa
//
LOCUS       DAVLTM010000028        18492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLTM010000028 DAVLTM010000000
VERSION     DAVLTM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.028"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            58..270
                     /locus_tag="VYN16_000862"
     CDS             58..270
                     /locus_tag="VYN16_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403417.1"
                     /translation="MTKFVKIQSCYRGHTEDELINIDDIGRICLGPNILFLRTPYNLG
                     EHHISITQNSVDKLLKVLDIIGEDGK"
     gene            267..638
                     /locus_tag="VYN16_000863"
     CDS             267..638
                     /locus_tag="VYN16_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403418.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFKEVPIVEEVKVEFEVE
                     ASTGRIYPETIIHLDISGYGNRVHSVHRFPGVFLTVGESIQLKILFYKRLRNFTADRF
                     LTFRESDWKFFIRDLVNEFVR"
     gene            709..1113
                     /locus_tag="VYN16_000864"
     CDS             709..1113
                     /locus_tag="VYN16_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1403419.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWTDVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            1194..1276
                     /locus_tag="VYN16_000865"
     tRNA            1194..1276
                     /locus_tag="VYN16_000865"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1225..1227,aa:Tyr,seq:gta)
     gene            1565..2005
                     /locus_tag="VYN16_000866"
     CDS             1565..2005
                     /locus_tag="VYN16_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1403420.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLSYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            1983..3281
                     /locus_tag="VYN16_000867"
     CDS             1983..3281
                     /locus_tag="VYN16_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1403421.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWHSRSLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYSWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAELRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            3293..4762
                     /locus_tag="VYN16_000868"
     CDS             3293..4762
                     /locus_tag="VYN16_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1403422.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSHLKKLKSYYM
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            4671..6308
                     /locus_tag="VYN16_000869"
     CDS             4671..6308
                     /locus_tag="VYN16_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1403423.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEI
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPENEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKITTDSQAENISLSKATSKINDALRYTTIFDPDTFAKEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            6310..6519
                     /locus_tag="VYN16_000870"
     CDS             6310..6519
                     /locus_tag="VYN16_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403424.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            6516..6929
                     /locus_tag="VYN16_000871"
     CDS             6516..6929
                     /locus_tag="VYN16_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1403425.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIHYLST"
     gene            6972..7217
                     /locus_tag="VYN16_000872"
     CDS             6972..7217
                     /locus_tag="VYN16_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403426.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKLDIFAETYEKTEE"
     gene            7219..7470
                     /locus_tag="VYN16_000873"
     CDS             7219..7470
                     /locus_tag="VYN16_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403427.1"
                     /translation="MLEKAKQLASQEFSRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGDYAEITHNGDKNETYVDVYAKVSNRAIKD"
     gene            7673..8242
                     /locus_tag="VYN16_000874"
     CDS             7673..8242
                     /locus_tag="VYN16_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1403428.1"
                     /translation="MNEETQTVETVEEQKVPAEPAQQPQDEKKYTDADVDAIIDKKFA
                     KWKSEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAKAMLSE
                     RDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPVDSDGI
                     TKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            8249..9094
                     /locus_tag="VYN16_000875"
     CDS             8249..9094
                     /locus_tag="VYN16_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEV1403429.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTDVAKEWTKGSELGADMVVSGTFGEVAGVQIVRSKKVDEGKGFIV
                     KVSPSQTQTDDANKYGAFVIMLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGEG"
     gene            9099..9281
                     /locus_tag="VYN16_000876"
     CDS             9099..9281
                     /locus_tag="VYN16_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV1403430.1"
                     /translation="MSLLLRRHYIQEEQVSQYSDLENKTLEELKNLAKEAGIAGAYKL
                     SKAEIVEVLEDLKSEV"
     gene            9271..9459
                     /locus_tag="VYN16_000877"
     CDS             9271..9459
                     /locus_tag="VYN16_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403431.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGGELEITEERYVELADNIASNGVA
                     ISDVLEKILPEPEFLEED"
     gene            9461..9772
                     /locus_tag="VYN16_000878"
     CDS             9461..9772
                     /locus_tag="VYN16_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1403432.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGERSRTEGGIAVVYGEKGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            9753..10091
                     /locus_tag="VYN16_000879"
     CDS             9753..10091
                     /locus_tag="VYN16_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403433.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHKVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            10066..10581
                     /locus_tag="VYN16_000880"
     CDS             10066..10581
                     /locus_tag="VYN16_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1403434.1"
                     /translation="MSELLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            10578..10946
                     /locus_tag="VYN16_000881"
     CDS             10578..10946
                     /locus_tag="VYN16_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403435.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            10949..11362
                     /locus_tag="VYN16_000882"
     CDS             10949..11362
                     /locus_tag="VYN16_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1403436.1"
                     /translation="MLANGITLSYGEAKGTYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFADGVGG"
     gene            11367..11747
                     /locus_tag="VYN16_000883"
     CDS             11367..11747
                     /locus_tag="VYN16_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403437.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVK"
     gene            11786..12136
                     /locus_tag="VYN16_000884"
     CDS             11786..12136
                     /locus_tag="VYN16_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403438.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEINGNS"
     gene            12123..15776
                     /locus_tag="VYN16_000885"
     CDS             12123..15776
                     /locus_tag="VYN16_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403439.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKRFSNGQSWDQLDFQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWNDVTSALSKVASWFGD
                     IFGKAFDAVKNAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            15785..16135
                     /locus_tag="VYN16_000886"
     CDS             15785..16135
                     /locus_tag="VYN16_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403440.1"
                     /translation="MAQLTINGVAVKPPKYFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            16140..>18492
                     /locus_tag="VYN16_000887"
     CDS             16140..>18492
                     /locus_tag="VYN16_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1403441.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQVIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGIEAKIQAQIEAADAAFDAEFDKREKAITDAIELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQAEALSRTKNELAGASTLIAQE
                     AKRIGLDSVARLEAFKLQTTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQVEV
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELSSKIASVQV
                     GGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVLDSPATGFDKAIRLTSSNARD
                     QIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNSGISPIFTLKDENWTKL
                     SFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLATSS"
BASE COUNT         5879 a         3296 c         4491 g         4826 t
ORIGIN      
        1 gcaaagttcg gctgggtgtt tgattgtgaa ggaattgata tagaggaggt ggaataaatg
       61 acaaaatttg ttaaaataca atcttgttat agaggacata ctgaagatga gcttatcaac
      121 atagatgata ttgggcgcat ctgtctaggc cctaatatct tgtttttaag aacaccttac
      181 aatttaggag aacatcatat ttctatcact caaaattcag tagataaact tttgaaagta
      241 ttggatatta ttggggagga cggcaaatga gaccttgtaa atatccatat tcaggaagaa
      301 gaaaaaagca agaaacaccg tcgccaatat tttctgcacg accaattttt aaagaagttc
      361 caattgtaga agaagttaag gttgagttcg aagttgaagc tagtacaggg cgcatatatc
      421 cagaaacgat aatacattta gatatttctg ggtatggaaa tagagtgcat tcggtacatc
      481 gtttccctgg tgtcttcctg actgtaggcg agtcaatcca gctaaagata ctcttttata
      541 aaaggcttag aaattttact gcggatcgtt tcttgacgtt tagagaatcc gactggaagt
      601 tctttatccg tgacctggtc aacgaatttg tgcgataaaa aagccaagac actctctgtc
      661 tcagctataa tctcaataat attattatac cacaaaagga gatagagagt gaacaaggct
      721 aaagagctct tgaaagagct acaggatctg gacatggaca ttcaaagccg tatagatgaa
      781 atcaatgagc ttgaggcagg tttactctca agccccaagt ggactgacgt caaagtccaa
      841 ggcggacaga ctagaaaagt tgatgacgtc tatactcagt tggtagtgat gaaagaggct
      901 atagagcagg atactaaaga ggtcattaac agaaaacttg aattaggtag aatgatcaat
      961 aggcttaaaa atccaaagta cagggcagta ttaagaatga cttacatcaa caaaggcacc
     1021 gctgatagcg tttgttatga tttgaatatg agtcgtacaa cctactacag gttaaaaaat
     1081 gaggcggtct tagctttgga agaagtcatc taacctcata gtgagcttat gggacttttt
     1141 ggaacagcac ggttctaaaa atctgttaga atggtagtat caagaattga aaagagaggt
     1201 ctcagaattg gtagatggtt acctgtaatg tcagggggct gtaatggcct tggaggttca
     1261 agtcctcccc tctcctttga gtgtttgtgt cccagaatga gttaaatctt ctgggtgggg
     1321 attcacatat cactcattaa cttaaaaatg gttgcggaag cgactggacc tcgcatgatt
     1381 gcgtagctaa ttatattccg gataagttat aagctagagg gtttgattcc ctcagaggtt
     1441 ttaaagacta caaaaaataa aaaaagaagt caaaatttaa tacgcacgca aggttgtagt
     1501 cgccttgcaa gaaggtcgca catcgtgtgg cttttttgat tgtttgaaag gtggtgatgg
     1561 aaaattgagt ggattgagaa taaaacaaaa gagatttgca gatgagtaca tcatctcagg
     1621 taatgcgacg gaagcctata agaaagcagg ttatcgtgtt tctagtgata gagtggcagg
     1681 cgttgaagga cataagttac taaagaatcc taagattaaa agttatatag atgaacggtt
     1741 gaaacagctt gattctgaaa aaatcgcaga ccagcaagaa gtccttagtt atctaacctc
     1801 ggtaatgcga ggagagacgc aagaacagac tttgataagc atcggagaat tgggtcaaac
     1861 gattacggat attgatgttg gagcaaaaga cagaatcaag gcagccgaac tattaggaaa
     1921 acgtcatagg ctttggacag acaaagtaga ggcagacgtt tctggaacgg tggtgtttgc
     1981 aaatgagtca gacataccag attaagcaaa acgatattgt cgttgactta cctaagacag
     2041 taggagctgg gtacggacag ttctggcact caagaagtct ttatcgtgta gtcaaagggt
     2101 cccgtggttc gaagaagtcc aagacaaccg ctttaaacta tgttgtccgt cttttgaaat
     2161 attcctgggc caacttgctt gttattcgta ggtattcgaa taccaacaag caatcaactt
     2221 atacggattt taaatgggcg tgtaatgtgt tgggggtgac tcatttgttt aaattcaatg
     2281 agtctttgcc tgaaataacc ataaaagcga ctggtcaaaa aatcctattc cgtggtttgg
     2341 atgatgaact caaaatcaca tctatcacgg tcgatgtcgg cagtctttgt tgggcatggt
     2401 tcgaggaagc atatcaaatt gagactgaag acaagttcag cacggttgtt gagtctatcc
     2461 gtggtagctt agatgtacct gatttcttta aacaaatcac agtcacattt aacccgtgga
     2521 atgagaggca ttggctcaag cgtgtattct tcgatgaaga gacgagacgg gctgacacat
     2581 tcgctactac aaccacttac aaatgcaatg agtggcttga tgaagtcgat atcaaacgct
     2641 atgaggattt gtatcatacg aaccccagac gtgctagaat cgtttgtgat ggcgaatggg
     2701 gagttgctga aggtttaatc tacgaaaacg tgactgtcaa ggatttcaat aaagatgaat
     2761 tactacaaga ttcagctaat aagttatgta tcggtcttga ctttggtttt actcatgatc
     2821 caactgcttt gtgttgttcg ttgataaatg acacgacgaa agagatttat gtctttgatg
     2881 aggcgtataa agtcggattg ataaccaaag aagttgcgaa gatgataaag gacaaaggtt
     2941 atcatcgctc acaaatcatt gctgatagcg ctgaattacg actgattgaa gagctcaggt
     3001 cagaacatgg catatctaga ataaaagaga gtcggaaagg taaggatagt attatggcag
     3061 gtgtatccaa attgcaagga tacgctattt atgtgcatcc agattgtaaa aacatcatgg
     3121 atgaatttta tagttactgc taccagcgag ataaagaagg caactggttg aataaaccag
     3181 aggataaaaa caaccacttg atggacgctt tgcgttacag ccttcaatgt atcgaaggtg
     3241 ggaaagcaac cgtccgcaga cgttctgatt atggtctata gagaggaaag acatgtacca
     3301 atatttaacc tatccacggg atggatatga tgagggttct ttgaagaaag acctgattta
     3361 caaattgata acgatacata acactgaaag ttcacatttg aagaagctta aaagctacta
     3421 catgggcgag catgctatct taaaacacac gagacgcaac gtgaacgcac ccaattacaa
     3481 gacggtagct aatcatgcca aggatatcgc agacacggct acgggctatt ttatgggcaa
     3541 tcctatcaag tataacaata ctgctgacgg tgatattgat gaactactta cagcctttga
     3601 tggtgctgag attgaccaag tagatgctca gaatgctttg aacatggcta tctatggtcg
     3661 tgcttacgag tacatctatg ctaaagaggg tatgactgag ttggattcaa ctagtattga
     3721 tccggagaat actttcatgg tctacgatga tagtattgag cggaagcctt tgtttgcggt
     3781 ctattactat gaagtaaaag acgatacgaa agacactacc aagcaccagg ctgaggtctt
     3841 taccgaaaat ctgcactatc acatggtgct gagaagtaca gattcaggaa caactcagag
     3901 cgaggaggca acacctcaca accttggtca aatcccaatt atcgagtatc gcaataatca
     3961 ctttgcgatt ggcgactacg agcaacaaat tagcttgata gacgcttata attccttgat
     4021 ggggaatcgt gtcaatgata aggaacaggc tgtagagtct atacttgtct tgtatggcac
     4081 gcagttagca gacactccag aagacgctaa ggtagcaatg aagattcttt ctgaagaagg
     4141 tcttttggaa ttgccgggcg atagtgcaag ggctgagttc ttgaagaata cgctggacga
     4201 aagtgctact gaaatcttgc gtacagctct taaagaggac atctacacat ttagccatgt
     4261 gcctaatttg actgatgaga atttcgcagg gaatacatca ggcgtagcca tggaatttaa
     4321 gctgatgggc cttgagatga ttactaagac caaggaagcg aactataagc gaggattgcg
     4381 tcagcgtatt gcgatttttg ctcattactt aggcatgaag cagattgctt tagagtctca
     4441 ttcaatcgtt ccacaattca gtcgtggttt gcctaagaac ttgttagaaa tctctcagat
     4501 tgtgaacaat ttggaaggca aagtgaccaa tagacagctt atttctctct tgccgtttgt
     4561 ggaagaccct gacgctgagc tggaagcctt ggaagaagag aaaaagaaga acatggaaga
     4621 catgccgatg ttcaacaaag acaacacgaa acccgaagac gaggtagagg atgaagaatc
     4681 aggagtattg ggcgaagagg aaagccaatc tgatttacca gcagatggac aaggccgaaa
     4741 agcaggcaga ccagttcgat aaggtctatc aggaagctaa gacttacttg gataaggaaa
     4801 tcaataagat tttcgataaa ttccaacgtg attatggtct aagtcaggta gaagctagac
     4861 aagtcttgaa gaacatgaaa gacaagaaaa atctgaatga acttcgtaaa gtacttgaag
     4921 cgagaccgaa tgacccgaac atccaaagat tactagctga cttagacagc ccagcttatt
     4981 ctttccgcat gaagcgccta gagcgtttga gtgatgattt agaccgtatg cgtgaatcta
     5041 tctatcattc agaaaagaca ggatcagatg ccttttatag cgacctgatg aaggatagtt
     5101 actacaaggc tacctttgac ctacaacagc agacaggact agcatatggc ttttctgggc
     5161 ttcctgagaa cgagattaaa catctacagt ctttcagttg ggtaggtgac ggaagtacct
     5221 actctacaga catctggaag aatacgggga agcttacttc tagcataaaa gatgaactac
     5281 ttatgagcct catgacaggc cgagatacac gagaaactgc acaagcaatt gctgagaggt
     5341 tcaatgtagg tcagaacgat gcaagacgtt tggttcggac agaatcagcc ttttttcata
     5401 accaaatgga actactcagc tatgaagaag cagacataga aaagtatatc tttgtggccg
     5461 tcttagacaa gcgtacatca cgcatttgtc aggagcatga caatcaggtc tatgataggg
     5521 ataaggctgt ccctggtgtc aattgtccgc ctatgcaccc ttggtgtagg tctactactg
     5581 tcggatacga tgaggacgca gactacagca agttgaagcg cagagcaagg aatccagaga
     5641 caggtaaagt tgagtacgtg cctgccgata tgacttataa agagtggtat agcaagtatg
     5701 ttgcgaaaga cggggaaaag gtgtataatc aagatacaag agaagccaag gcgaaatttt
     5761 atagcgaaca actattgtcc aaaatttcag gagttgagcc aaaaattaca agtgatatgc
     5821 aacgtatcgc aggagaaaac aaattggcag gtcttgaatt taggaagaaa acagttgagt
     5881 cattatcacg taaaattaca acagatagcc aagctgaaaa tataagttta tcaaaggcta
     5941 caagtaaaat taacgatgct ttgcggtata caactatttt cgatcccgat actttcgcaa
     6001 aagagtattt gaagatgaaa cagaagctta tcgcagaagg ttataaaatt gtaaaagtaa
     6061 aaaacacttg gctagtagat ggaccataca aaggtgtgaa tacagtcgtt gaaaaagatg
     6121 gtatcaactt tgaaatgcag tatcatactc aggaaagttt cgacttaaaa aatggttcat
     6181 tacatgaact ctatgagaag tatcgtgata cgaatacatc tgatctagaa cgcatgaaat
     6241 tatttaagga aatgcttgat ttaagcaatg ggcttgagat tcctaaaaat atagagaggg
     6301 tgaagtgata tgaaagatat taaatactac cgcacaacga cgaacaatgc tcaagtactt
     6361 cgtttgattg atggtgtcat gcaagttttt gacattgaaa aaaagtgggt taatagcatg
     6421 gattggttta ataaaatctt ttttaatgac tttacggatt ttgaagaaat ttcagaaaat
     6481 gatgcattta cttatattga caggatggta gcggcatgat tgatattgcc ttggctatcg
     6541 ctaaaaaagc acatgcaggg caggtagata aagcgggtgt tgattacata cagcatcctc
     6601 tctatgtggc cagtcaagtc aacactgaac aagaaaaagc tgtcgctctt ttacatgatg
     6661 tgattgagga tagcgatata actgctgccg atttattcgc gtctggcttg tcaaatgaag
     6721 ttgttacagc ggtacaaatt ttgacaaaga aaaaaggtca aagttatcaa gaatatcttg
     6781 ggaaagtaaa atcaaataat ttagcaagag ttgtaaaact tgcagatttg aaacataact
     6841 cagatttatc acgtttgaaa tctgttacca atacagacta cgagcgtgtt aaaaaatata
     6901 aaaatgcaat tcactactta agcacctaga gagatctaag tgcttttttc gtacccagaa
     6961 aggagtgagg aatgaaatac cgtaaaaaac cagtagtggt cgaggctgtg cagtggaacg
     7021 gcaataacca taaagaggta attgactttg cagaaaataa gatttggttt gatgcacttg
     7081 ggaatatatg gattgctaca cttgaaggtg atatggtagc taaaaaaggg gattatatta
     7141 tcaaaggcgt gcaaggagaa tattatccat gcaagctgga tatttttgca gaaacatatg
     7201 aaaaaacgga ggaataaaat gttagaaaaa gcaaaacaat tggcatcgca agaattttca
     7261 cgcttatcag gtcgtgaaat caaagcagaa gactgctttg tagtttggtt tagcaagacc
     7321 ctgcaaaact ggaaagctct tgttagtacg aacgcaatta catcaagcga accttgtgga
     7381 gattatgcag aaattacgca taacggagat aagaatgaga cttatgtgga tgtttacgcc
     7441 aaggtttcaa atcgtgccat taaagattag gaggtgatcc aacatcttga cttgcaggaa
     7501 tagactgcta taaattactg taaattgcta taaaccgtgt catatttgat gcggttttcc
     7561 tatgccctaa ccgtatggaa tcccgtacgg tttttatatt gtccaagcat tgacgacact
     7621 aaaagctatg gaaattacag tcggggacga ctttaaaaat aggaggttcg caatgaacga
     7681 agaaacacaa acagtcgaaa cggttgaaga acaaaaggta cctgcagaac ctgcacaaca
     7741 accgcaagac gagaagaagt acacggacgc agacgtcgac gctatcatcg ataagaagtt
     7801 tgccaagtgg aaatcagagc aagaagctaa agaaaacgaa gcgaagaagc ttgctaagat
     7861 gaacgctgat gagaagcaga aatatcagtt ggatcagcgt gagcaagaac tagccgaccg
     7921 tgaaaaggcc attgctcgca aggaattgac cgcagaagct aaagcaatgc taagtgaacg
     7981 tgacttacct gttgagttag taaatgtagt tgatttgaca agcgcagaga cggtatcgca
     8041 gtctgtcgct gtattgcaga aatcatggga gcaagccgtg caaaaaggcg ttcaagaaaa
     8101 actaaaaggc ggagctccaa tgaaacaagc gccagtcgat agtgacggta tcacaaaaga
     8161 agaatttgct cgtatgggtt atcagagtcg aaatgagctc tatcaaaaga acccagagct
     8221 ttataagaaa ttgaaaggat aatagaaaat gacagcagga caaactaaat tagccactat
     8281 ggttaatcca gaagtaatgg cggatatggt agccgctaaa ttacctaaat tgattaaatt
     8341 tacaccgcta gcgtatgtag agacaaagct tgaaggtcaa ccaggtagca ctttaacagt
     8401 gccagcatgg gagtatgcag gagacgctac tgaaattgaa gaaggccaag caattacgcc
     8461 agaccaattg actactaaaa agactactat gaccatcaaa aaagcaggta aaggttatga
     8521 aattaccgat gagtctcttt tgtcaggtct tggtgaccca ctaggtcaag cgacttacca
     8581 gcttggttta gctattgcca acaagatcga taatgatttg gtagcggtag ctaaaactgc
     8641 aacacaacat attacagaaa ctccaacaac tcttgaggca atcgataaag ctctagatat
     8701 ctttgaggac gaagaagatg cacagtatgt tgctatcatc aaccctaaag atgctactaa
     8761 gctaaaaact gacgtagcaa aagaatggac taaaggttca gagcttggtg cagatatggt
     8821 tgtatccgga acgttcggtg aagttgccgg tgtgcaaatc gtccgttcta aaaaagttga
     8881 tgaaggtaaa ggatttatcg tcaaagtctc tcctagccaa actcagacag acgatgccaa
     8941 caaatatggt gcgtttgtta tcatgctaaa acgtgatgtg gctatcgaaa cagaccgtga
     9001 catccttaaa aagacaacgg ttatcactgg tgatgaacac tatggtgttt acctttacga
     9061 ccctacacga gttgtaaaat tcggtgaggg gtgacggcat gagcttattg ctacgacgtc
     9121 attatatcca agaggagcag gttagccagt attctgattt agagaataag actctagaag
     9181 agttgaagaa tctagccaaa gaagctggca tagctggcgc ctataagtta tcaaaagccg
     9241 aaattgtaga ggtgctggag gatttaaaaa gtgaagttta aaattaaaca agatttctat
     9301 gattgggaat caaatgtgaa acgactggca ggaggggagc ttgagattac tgaggagcgc
     9361 tatgttgagc tggctgacaa tattgccagc aacggtgtcg ctatctcaga tgttcttgag
     9421 aaaatcctcc ctgaacctga gttcttagaa gaggattgat atgtctatag agttgctgaa
     9481 gaaattaaca ggcgaagaag atactcagct tctcatgttg ctccaaacaa gggctacaaa
     9541 tcttatcttg tcagagacta atcgcacatc tttgacacct gctttaagtc ttttgatacc
     9601 tgaggttgct atcgagctcc acaaccgctc aggagcggaa ggagagcgtt ctagaaccga
     9661 aggtggtata gcagtagtct acggagaaaa aggcctgtct acgggtcttt tacagcgtat
     9721 acgcatgcac agattagcaa gggtggcagg ccatgttttt gaagcagagt agactgaaac
     9781 cttatccgat gcgacggttt gaaaagactg tcacagagga aggtgtcgca aaagaagggt
     9841 atgccaagga agctgagaca gtccgtcttg aattgtggcc agctagtagc aagttgcaat
     9901 ctgaattgta tggtgagcgt gtcaatgata ttttgaatgc caatgccaac aaatcagcta
     9961 ctatcaaagt gaaagatggt gtgtgtatcg atagccagac agaagtgact cataaggtca
    10021 tttctaaaaa ggtctacaca catcatcaag ttttggagtt agagcgtgtc agagctacta
    10081 ggggcagata ggcttatagc taaatgtaga cgattggcta gtaaaaaaac tggcgaggat
    10141 atcgtcttac gtgcggtaca caatgctact ataaaggttg tccaagcaga tgcaagaaga
    10201 ctcgcaccag cgagagatgg agagcttata actagtatca aaactagggc aaaaatggac
    10261 ggagataagg ctataggcga ggtttacacc aacctaaaat acgctcctta cgttgagttt
    10321 ggaacgggac caaaaggaca agctagccat tctggtatct ctccagaggt cagcgtaact
    10381 tacaagtcta gtccgtggta tgtgcatgaa gaccaaatca atgtaggacc ttaccacttt
    10441 caaaagattg gggagttcta caagatgtat ggtcaacctg cccagcctta tctttatcca
    10501 gctttgagag acaatcaaga gcgtgtgtct aagaatattt cgaattatgt ccgtagaaag
    10561 ataagagaac aaataaaatg attaatatca agcctgttat ttataaagaa ttgcaaaagg
    10621 tcgcagataa tgtgactgat acttatccta gcgattggga gactttccca gtcgttattt
    10681 ttttagaaga acaaaacaag ccgggtgatt ggtttgatga ccaggaacaa aaatcctcta
    10741 tccgctacaa ggtggatatc tttgatgata ccagcactag tgagttagct gttaaaatca
    10801 atcagatttt tgagtcttta ggtttgcgaa gaaccgactg ccaagacgtg ccagacccgt
    10861 ctcatttgag acataaggtc atgcgttttg aaggtgtcgt tgatttagac tcagagcttg
    10921 tttttcaatt tagaatggag aattaaacat gttagcaaat ggaattacgc tatcttatgg
    10981 cgaagctaaa ggaacttata ctaaacttgt tggattgaaa gaagtgccag agtttggtat
    11041 tgaactcgaa aaagtagaaa atactactct tgaagataca gtgaagaagt acgagtttgg
    11101 tattggggac ataggagaac ttgagtacaa gttctcttat aataattcaa gcgcaactgc
    11161 tccttatcgt gtattgcgta aggcagcaga cgacaaggaa aaactctact tcgaacaagc
    11221 ttatccagac ggtactaagg tcacatttga aggtcaagta tccgtaaaac ttggcggtgg
    11281 cggtgtcaat gccgttatcg atttcaccct taaaattgct ttacagtcag agttggaatt
    11341 tgcagatggt gttggaggtt aattaaatgg cgttaaaata cacaacttgg aaagttactg
    11401 acgaaaaaga gttgaagcta cgtttgacat ctcatcaagc tgcaactgtg gaagaaaaaa
    11461 tcggcatgaa cttgctgaag attttcatgc ctgaagcagg cgaagagttc actttgccac
    11521 ctttgaaagt tatgttgttg ttagttcatg gagccttgca gcagtatgaa catgggtatt
    11581 cctttgaaga tgtctacgac ctatacgatg aatacgtgga caatggggga gaccaaacaa
    11641 cattcatgac agaggtttta atgccactct ttgaagtatc gggttttact ccacgaggaa
    11701 gcaaggacaa gaaaacttcc aagaagaaaa tgacagtagt caagtaatct taacggtaac
    11761 tcagattatt gagaggcttt atcctatgtt tttggacatc gggggcaagc ctcttgattt
    11821 ttgggatttg acggtgcttg aaatcaggga aatgatagaa agctacaacc gtgtcaaaat
    11881 ccaagagcgt aaagaaaaga ttattgactc atacagactt tcgcagatga tatccaacca
    11941 cgtttcttta ttgttatcca aagatgccaa ggcctttgag ttctgggaat atgcgcctga
    12001 gttgtttgta gaagaacaac aagcagtaga acaggaacga cagagacaag cgcttttgtt
    12061 gcataaggaa cggatgcgtg attttgcaga gagacacaat cgaaaaagga aggaggaaat
    12121 aaatggcaac tcttgatgaa ttgaaggtca tgattgacgc tgagatagcg cctttcagga
    12181 agaagatgaa agaagtcgag aatcaggtca aaggaacatc tgaccaagtg aaaaatgcca
    12241 ctgccaaagt tcgtgaacag tcgaactcaa tcggtagtgc gtttggtaag ctggctaagt
    12301 ttgctggttt tgcaatcctt ggtaagaaat tacttgatgt tgggatgtat tcaacgcaga
    12361 cagctcttga agtgtcagcg tctatgaacc aaatcaagcg acagatgggc gagagttcgc
    12421 aatctttctt aaaatgggtt aacgataacg ccaacgctat gaatatgggt gtgggtgagg
    12481 ctactaacta cggtgcagtc tactcaaacc tattttctgg atttattaag gacaccaaca
    12541 agctaagtgc ttatactgct aagatgttgc agacatcggc agtggttgct gaaggctcag
    12601 ggcgcacgat tacagacgtt atggagcgga ttcgctcagg tttgctaggg aacaccgaag
    12661 caattgagga cctaggaatc aacgtcaatg tggctatgat taagtccact gaagctttta
    12721 aacgtttctc aaatggtcaa agttgggacc aactcgactt tcaaacccag caacaaatcc
    12781 gtcttatggc tatcctggag caagctacag ccaagtatgg agataccttg tctaattctg
    12841 taaatggtcg tatcagcctg tttaagtcgc taatgaagga cgcagcattg aaccttggta
    12901 actctatgtt accgattatc aatgccatta tgcccgtctt aaactctttt gcgatggtct
    12961 tgaagaacgt gactgctaaa ctcgctgagt ttatcgcttt gatgttcaac aagaaagcta
    13021 cagttaaaga tggtgtcggt ggagcagttg gagacatggg gaacgccatg aaagacgctg
    13081 caggcggagc aggagacctt gctgacgcag tagacgacgc tggagattca gcaggaggac
    13141 ttgctgacaa tcttggagac tccgccaaaa acgctaagaa agctgctaaa gagttgctag
    13201 gtcttttggg atttgatgag attaacatct tgcaaaaacc aaaagatgac gacgcaggcg
    13261 gttctggagg cggtggcaaa ggtggtaaag gaaagggagg cggtggcgga cctttcaaag
    13321 acatcttgcc agaagtcgag ttgaccgaca tggacaacaa attcaagagc atttttgatg
    13381 gtcttggaga taagctcaaa gggttgtttg acctcttcaa gaaaggtttt gatgcagcat
    13441 ttagaccaga aggtataaaa cgcattaaga ctgccttaga ccaaatagct aagacaatgg
    13501 gagaaatcgc cactgaccca agggttgtga atgcctttaa ccgaatggct gagaaaattg
    13561 cttatgcttt agggcaagtg acaggctcaa taaccactat cgggctaggt atcggtgttt
    13621 tccttgccga aagtattgca aatggccttg gaaggcaaaa agaacgcatt accagggcgc
    13681 tagtcgcttt gtttgataat gttggtaacc tttccgaggc agtaggaaac atagctcagg
    13741 acttttctag tgctttctac gacgtcatta cctcaactgg tgcggttcgt atcggtagcg
    13801 ctattgtgtc aactctgttg agtttgacat ctaccattgt tgaagttggt agtaaattag
    13861 caggaagttt gtttaaaggt tttgaaaaag tcgttgtgac aagcgctcct aaaatttcat
    13921 cagtcttcca aagtttatta gatactgttg cgcctgtatt tgagagcatt gaaaggtctg
    13981 ttaacaaatt tggcgatggc ttaagtcgtg tttatgatga acatgtagtc cctgctatta
    14041 actctattgc taatgctttt aatgggctaa ttgacattat tcagattctc tgggagaatt
    14101 cctggcaacc ttttgctgag tttttatcag gagtattcgg tgttagtatt gaaggaattt
    14161 cagatttatt aggaggtggc cttttagcca ctttgggact attggcggat gctattaagt
    14221 tagtggcaga tggtttcacc gttttttctg actggtgtaa agaaaacaaa gaacctatcg
    14281 tagctttgat aacaacttgg caaacgatta atttcttatc atgggcagaa caagctggag
    14341 gacttgcagg agcattcagc ttgttaggta gtaaggtctc tttgattgtt ggagggatta
    14401 agaatctagg tcttgctatt aaagcattga catttgataa gttggtcagt tttggtgaaa
    14461 caatctattt gaacacctta tatgcaaaag attttgtggt caattcaggt aaaacaattg
    14521 cacagctagg aaaaactgct ttagaacttg gtaaatcagc tctagcatgg actgctcatg
    14581 cagcgaaaat gggattagca accgcggcgg aatttgcaca ttctgttgca gcaggagtcg
    14641 ctacagctgc aacatgggct tttaatgcag cgttagcagt tttgacaagt ccaataacat
    14701 ggattattgc agcaatcgca gctttgattg ctattggtgt tttgctctat caaaactggg
    14761 acactgttgt tgagtttgct aaaactgcat ggcaaggact atgtgatttt atcagtggta
    14821 tttgtcaagc gattggcgaa tttttcagcg gtctatggac gaaactacaa gaaatctttg
    14881 agccgatagg tcaatggttt ggcgagaaat tccagcaagc atgggacgcc attgtaaaca
    14941 tattctctgg catcggagag tggttctctg gtgtattcca aggtgcatgg gacgctatcg
    15001 ttaatatctt cacaccaatc ggctcatggt tcggacaacg ttgggcagat gtgactagtg
    15061 cgttggctaa tatcggggca tggtttactg acatgttcca aaaagcatgg actggcttaa
    15121 caaacatctt tagcaaacta ggttcttggt ttggcgagag atggaacgat gttacaagtg
    15181 cactttccaa agtagcaagc tggtttggcg atatattcgg aaaagctttt gacgctgtta
    15241 aaaatgcctt tagctctatc ggagacttct ttaaaggcgt ttgggatact gtcaaaagta
    15301 tcttcgttaa tgctggtcag atggtcggcg aggcagtagg tggagcgttt aagagtgcgg
    15361 tcaatgcggt tcttggaacg attgaaaatg tagtcaatgg cttcatcgga atgattaatg
    15421 gagttttagg cgttgtcaga aacttacctg gtctaggatg ggttggtagt gtaagtacag
    15481 ttagcctccc tcgtcttgcc cgtggtggta tcgtcgatag tccaacaatc gccatgattg
    15541 gtgaagctgg taaagaggcg gtcgtaccac ttgaaaatac aggatttatc caaacacttg
    15601 gacgagtagt cagcagtgcg gtagtaaatg ccatggctgg tgttagtcca caaggtggat
    15661 tctctggcga cggcgacatc gttatccaaa tcgcaggcca tgagttcgga cgggtagcca
    15721 tccaagaaat caacaaggag catgaacgag caggtcaaac cttgctcaag atttaggagg
    15781 ttaaatggca caattgacaa tcaatggggt ggctgtgaag cctcccaaat attttcaagt
    15841 cggtattcaa gatatcgatg gagagacagg gcgtaatgcc aatggcgaca tggtgcgtga
    15901 ccgtatcacg accaaacgca aactagactg tgaatggggt atgatgactc agggagaaat
    15961 aagtcagctt ttacatgctg tatcatctaa attttttgag gtatcttatc cagaccccat
    16021 ggatggccaa gtcacaaaga ctttctatgt cggtgatagg accgctccta gctatacctt
    16081 tactgagaag tttaaacctt ggtctggcgc taaatttaat ctggtagaga ggtaagaaaa
    16141 tggacgcttt aactagacga caatttgaca gagccatgtt tgccaaggaa aggacgctgg
    16201 ctattcgtgt tggtgaatat gcttcacggg atatcaaaga ggctagtttt gagtatggct
    16261 acatcaaggg cgatacttat aagcctggtg gaacctgcgc tggtagcggt aaaattacct
    16321 ttaccagtat cattaccacg ttcaataagc tggataccct gcaccctgag attggtctac
    16381 tggttgggga tacctaccag tgggtcaaga tgggggaata cttcatcaac gatattgaga
    16441 ttgaccgaaa ccgcaacaca accacacttg aacttatgga cggtatgttt aagctcaatc
    16501 gtgagtacgt gacggatttg catttcccag ctgaagtacg agaggttatt caggaaatct
    16561 gcctgaaaac aggcattgag ttagcgaatg actatttcgg aatcagcgcg atgcgttatc
    16621 atattgagca agttcctgag ggtaagaaac tttcctttag ggatatgctg agcgctatga
    16681 ctcaggtgat tgggatgtct tgtttcttca acagagaagg caagatggaa atccgtgatt
    16741 tgactgagtc caatatcacg atcaacgctg acagttattt cttgcatggc ttgaccaaga
    16801 gtgagattga gtatcagata gctggtatca cttgtaagac ggacaagaag tctctgacgg
    16861 tcggtatgaa gacaggccgg tctttggaac tggacaatgt cttcatgacc cagagcgctt
    16921 taaatgacct gtattacaaa ctgaaaaacc taacttacta tccgtataat ctcaactacc
    16981 aagggcattt actgcttgag gttgggcagt gggtaaccat tcagaccaac aagaaagaaa
    17041 cctttaaagt tcctgtctta agtcagagct ttactttcaa aggtggtcta agaggtcgta
    17101 tcagcgcaga tagtaaagct ggaaatgata cgcagtattc ttacgagggt acgattacca
    17161 agcagattaa gcaacaagat ggcattgaag ccaaaatcca agcgcagata gaagcagcag
    17221 acgcagcctt tgacgctgaa tttgacaaac gtgagaaagc tatcacggat gccatcgaac
    17281 ttgccaaagc cagagctgaa gaagtcaaga gagaactgtc tgacactatc aatcagcgct
    17341 ttaatagctt tgacaacggg ccattgaaag aaactaagcg caaggctgag gaagctttgc
    17401 gaaatgctgg cgcaagtacc ctgcttgcac aggaagctaa gcggattggg ctggattctg
    17461 tcgctagact tgaagcgttt aagtcgcaga ctacgagcgc acaaacggct ctgtcgggtg
    17521 acttggacgc tctgaaacgg actatcgcga atgatattcg accgaagcaa gcacaggctg
    17581 aagctgagat tgccaagcaa gctgaagcac ttagccggac taaaaatgaa ctggctggcg
    17641 caagtaccct aattgcacag gaagctaagc ggattggact ggattctgtt gctagacttg
    17701 aagcgtttaa gttgcagact acgagcgctc agacggcttt gtcaggtgac ttggatgccc
    17761 tgaaacgaac tatcgcaaac gatattcgac cgaagcaagc acaggctgaa gctgagattg
    17821 ccaagcaagt tgaagtactt agtcggacta agaatgaact ggctggcgtg aagtcagcgc
    17881 aagcgacgta tgaggagacg acgactcgta gactgtcaga actgaccaac ttggccaatg
    17941 gtaaagccag caagtcagaa ctcacgcaga cagctgagga gctctctagt aagatagcga
    18001 gtgtgcaggt cgggggtagg aattatatcc ggggtacaaa gcgcatgatg ttagccagag
    18061 gattgtgggc atcaggtact tttagaccgt caggcgctgg gacggcaaag acgattgatg
    18121 tattagacag tccagcaact ggctttgata aagcgatacg attgacctca agcaatgcta
    18181 gagaccaaat aggtattgct caagatggat tttatatctc gcaaggcaca tacacgatgt
    18241 cttgttgggt caaaggcaga agaggtcaaa aggtcaagct acaaacttat tggcaagtca
    18301 atgataattc gggtatttcg cccatcttta cattaaagga tgaaaattgg acaaagctat
    18361 cgtttactag cgctagaaat agggctggag tcgcatcaat tggctatgtg tatctcgtaa
    18421 atgctgaagt cggagaatat ttagatgttc ttgcgcccca gctggaagac ggaagtttgg
    18481 caacaagctc aa
//
LOCUS       DAVLTM010000029        18309 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLTM010000029 DAVLTM010000000
VERSION     DAVLTM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.029"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(125..739)
                     /locus_tag="VYN16_000888"
     CDS             complement(125..739)
                     /locus_tag="VYN16_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1403442.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAILGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1554..1823)
                     /gene="rpsO"
                     /locus_tag="VYN16_000889"
     CDS             complement(1554..1823)
                     /gene="rpsO"
                     /locus_tag="VYN16_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1403443.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1964..2592)
                     /locus_tag="VYN16_000890"
                     /pseudo
     CDS             complement(1964..2592)
                     /locus_tag="VYN16_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            3451..3774
                     /locus_tag="VYN16_000891"
     CDS             3451..3774
                     /locus_tag="VYN16_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1403444.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3832..5775
                     /gene="thrS"
                     /locus_tag="VYN16_000892"
     CDS             3832..5775
                     /gene="thrS"
                     /locus_tag="VYN16_000892"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1403445.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5824..6798)
                     /locus_tag="VYN16_000893"
     CDS             complement(5824..6798)
                     /locus_tag="VYN16_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1403446.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LIQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6791..7468)
                     /locus_tag="VYN16_000894"
     CDS             complement(6791..7468)
                     /locus_tag="VYN16_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1403447.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7624..8697
                     /locus_tag="VYN16_000895"
     CDS             7624..8697
                     /locus_tag="VYN16_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1403448.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            complement(8788..9090)
                     /locus_tag="VYN16_000896"
     CDS             complement(8788..9090)
                     /locus_tag="VYN16_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403449.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9159..9596)
                     /locus_tag="VYN16_000897"
     CDS             complement(9159..9596)
                     /locus_tag="VYN16_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1403450.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9672..10514)
                     /locus_tag="VYN16_000898"
     CDS             complement(9672..10514)
                     /locus_tag="VYN16_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1403451.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10626..11276
                     /locus_tag="VYN16_000899"
     CDS             10626..11276
                     /locus_tag="VYN16_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1403452.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11440..11626
                     /locus_tag="VYN16_000900"
                     /pseudo
     CDS             11440..11626
                     /locus_tag="VYN16_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11893..12336)
                     /locus_tag="VYN16_000901"
     CDS             complement(11893..12336)
                     /locus_tag="VYN16_000901"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1403453.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(12366..14588)
                     /locus_tag="VYN16_000902"
     CDS             complement(12366..14588)
                     /locus_tag="VYN16_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1403454.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            15604..16233
                     /locus_tag="VYN16_000903"
     CDS             15604..16233
                     /locus_tag="VYN16_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1403455.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(16356..18248)
                     /gene="pepO"
                     /locus_tag="VYN16_000904"
     CDS             complement(16356..18248)
                     /gene="pepO"
                     /locus_tag="VYN16_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1403456.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5384 a         4170 c         3413 g         5342 t
ORIGIN      
        1 aaatcaaaag ccactctaca atagagtaac tttaagattg tccatttata ctcaatgaaa
       61 aagccctctg aaaatcagag agctgatttg agctcgggct aaaatcctag tgaaacaaaa
      121 aaatctacac gatcagaaaa gtctctatcg tgccattttc atacatgatg tatagtccaa
      181 gtagaatgaa tactaatggc acaatgattc gctcgtattt ttcaattgtc tcgaatatta
      241 acggaataga ggataacacc cgactaatct cgcaaaagat aattatgccg attacaaaca
      301 caagcaaggc cacgagggtc tgtgaccaat ctaacgaagc aaaataaggt atatagatac
      361 ctaaattatc tccgccagac gcaattgtca gcaatgtaac tgtccaaaac agttgatttg
      421 ccttgctttg ttccaatctt tcaataattt cttcctcttc ttcttccgca tcttctccaa
      481 gaattgcaaa gcgaatccct aaatagatag ggattaaacc aagcaatcca accatccatt
      541 cttcaggcac gaaattaacg acataagcag caactaaact cgcccctaca agtaagcctg
      601 tgcctagata ttgccccgca taaatatgcc atttctgttt attctgtgat agttgtgcaa
      661 ataaaataat taaaataatt aaataatcga tactggtgga aatataaaca ccaatagcag
      721 atatgattgt ctgtcccata aaaaaacctc ctgtattagt cagtaataaa tcagcagatt
      781 ttaggagagc acagacacat taatttagct atcgctagtg ggtaatgtcg aacgtaggaa
      841 agcattttac tcctcctcaa aacgtagtta caaagtaatt tctaactgga attcagtgac
      901 tacttccatg taaagtataa catactatac tttactttgt aaagagtggg ctcttctagg
      961 gggcttgcat acagctactc ggcttgaaaa gccgagtaac tgcgcacctt tatggtgtaa
     1021 ttagctgata ccatttgagg tttttgtagt ctccaaaatt gtgaccgata aaacctcaat
     1081 aaaaaagccc tctgaagtca gagagctgat ttgagcccgg actaaaatcc tagtgaaaaa
     1141 gatgaaactc cttgcgttca tcgaacactg tgccatttcc ctattttcat acggattttt
     1201 gacgccctta gcatcatgat tcttgctgga taaatcgttt atagactagg cgacgaggcg
     1261 aagatcgtac gaaactttca gtgaaacaaa aaaaatctcc ccgaagggag aaaagctggt
     1321 ttattttacc ttacagtgct aggaaccgta accaaagatt atacttgagt atatcgaggt
     1381 aaggtgacaa cgcaaaaaag ctacccaatt tagagtagct tcattatcaa gatatgctca
     1441 attgaacacg gcctaagcac ttggcaaaaa agataaaatc tcctagaaac tgaagtttct
     1501 tcgtcaattt tcctattttt gctttgtgct tttgacagca tttgtatctt gaattagcga
     1561 cgaagtccta gagagttgat caactcacgg taacggttaa cgtcgttttt acgcaagtat
     1621 gcaagcaagt tacgacggcg accgattttt ttcatcaatc cacggtaagt agcgtggtct
     1681 tttttgtgtt gtttgatgtg ttcgttaagg tggttgattt cccaagtaag gacagcaact
     1741 tgaacctcta ctgaacctgt atcaccttcg tgacgtgcat attgtgcaat gatttcattt
     1801 tttttctctt ttgagattgc catgatgttt ctccttttta tttggcttca tccgagtgac
     1861 aggttggcat gcctgtaacc aagaagaagt tatttgtctt tacgacaatc cctattttac
     1921 caagatttaa attttctgtc aaacattaat actctcatag ttattatttt tcaagtaaag
     1981 agatacgttc ttttatatga tgaccttttt caagttcatc taccaatcca atagcataat
     2041 ctgcataact aatctggctt attccttttt cattagtagt aaaaatttct cctgctagaa
     2101 tgtagtttcc tgttttttca ccatcgggaa taaaatctac agcaggactg acaaaagtcc
     2161 agttgagatt attttttgta cgaagaagat ccaattcatc cgcttgagct tttgctagtg
     2221 acttaaattc ttcaggaaaa tcagacgtat ctaaaagtcg tgtcgttttt gtttcatcta
     2281 tataaagact ttctgctccc ctacaactag aaaacgtgtc tgtgttccag caagaagctg
     2341 attaaatagt tcgattgatt tgctgtggag cggtagcgta tctggtgtat aagcaccaaa
     2401 cgctgaaata acagcatcaa atccagtaag atcatctttt gtcaactcaa ataaatcttt
     2461 tttaataata gactcagctt gacttttgtt ttcagaacga acaatagccg ttacttcatg
     2521 tcctcgtttg actgcttctt caacaattgc tttccccgct tgtccatttg ctgcaataac
     2581 tgctagtttc attttttata cctctattgt tgtaattatt ttagttacaa caaatgtgac
     2641 actcttaata atcaatatca atagtcttgc ttaattatta tcaaaatatt tctaccaaga
     2701 aaactaacta tgattctagt gaaaagaaaa atcttctttg tcaacaaatt tactttcttg
     2761 ttttaaacat gctataataa tcatagcaag agatctaagt tgtctgtttt tttaaaacga
     2821 ggtgattatc atgcgtagat tctattccca tctcccctac tatctggtca tattattctt
     2881 ttattagcca ctttatgagt tgttcttgct agttgtttct gaccccttta cactatataa
     2941 acaatcttct cttctttaca cctctggtaa tcttgattgt atcgttactc tatagctacc
     3001 gtttccgttt ctcactttga tggttagttg gtaacggact gctcttttac tttactatca
     3061 taacctttgg tgagtttata ctaatttact tgctaatcta tgaaacagtt gctctggtgg
     3121 gcatggattc tggtattagc atcaagcata ttctacaaaa tatgaaaaac aaaaaacttt
     3181 cacaaaatcc ttgaaaaatc tcacaatcat gctataataa tccatagaga caagtcactt
     3241 agtccctttc tactagagag tgcgtggttg ctggaaacgc ataggaagtc taaactgata
     3301 ctactcttga gttttttatg aaaacataaa acggtggcca cgttagagcc gatcagaggt
     3361 gtccctctct tttgaggtac ataaatgaag gtggaaccac gttgcgacgt cctttcgagg
     3421 atgtcgcatt tttttattag gatactaatt atggagttgc aagaattagt ggagcgcagt
     3481 tgggcaatcc gacaagctta tcacgaactg gaagttaagc atcatgattt caagtggacg
     3541 gtagaagaag acctcttggc tttatctaat gatattggaa atttccaacg actggtgatg
     3601 acaaagcaag gacgctacta tgatgaaaca ccctacacac tggaacaaaa actttcagaa
     3661 aatatctggt ggctattaga actttctcaa cgtttggata tagacattct gacggaaatg
     3721 gaaaacttcc tctctgataa agaaaagcaa ttgaacgtta ggacttggaa gtagtctgct
     3781 gataaaaaat caatgcttag aaactatgaa ataataaaaa aggagaacat catgattaac
     3841 attactttcc cagatggcgc tgttcgtgaa ttcgaatctg gcgtaacaac ttttgaaatt
     3901 gcccaatcta tcagcaattc cctagctaaa aaagccttgg ctggtaaatt caacggcaaa
     3961 ctcatcgaca ctactcgcgc tatcactgaa gatggaagca tcgaaattgt gacacctgat
     4021 cacgaagatg cccttccaat cttgcgtcac tcagcagctc acttgttcgc ccaagcagct
     4081 cgtcgtcttt tcccagacat tcacttggga gttggtccag ccatcgaaga tggtttctac
     4141 tacgatactg acaacacagc tggtcaaatc tctaacgaag accttcctcg tatcgaagaa
     4201 gaaatgcaaa aaatcgtcaa agaaaacttc ccatctattc gtgaagaagt gactaaagac
     4261 gaggcacgtg aaatcttcaa aaatgaccct tacaagttgg aattgattga agaacactca
     4321 gaagacgaag gtggtttgac tatctatcgt cagggtgaat atgtagacct ctgccgtgga
     4381 cctcacgttc catcaacagg tcgtatccaa atcttccacc ttctccatgt agctggtgcg
     4441 tactggcgtg gaaacagcga caacgctatg atgcaacgta tctacggtac agcttggttt
     4501 gacaagaaag acttgaaaaa ctaccttcaa atgcgtgaag aagctaagga acgtgaccac
     4561 cgtaaacttg gtaaagagct tgacctcttt atgatttcac aagaagtggg acaaggtttg
     4621 ccattctggt tgccaaatgg tgcgactatc cgtcgtgaat tggaacgcta catcgtaaac
     4681 aaagagttgg cttctggcta ccaacacgtc tacactccac cacttgcttc tgttgagctt
     4741 tacaagactt ctggtcactg ggatcattac caagaagaca tgttcccaac catggacatg
     4801 ggtgacgggg aagaatttgt ccttcgtcca atgaactgtc cgcaccacat ccaagttttc
     4861 aaacaccatg ttcactctta ccgtgaattg ccaatccgta tcgctgaaat cggtatgatg
     4921 caccgttacg aaaaatctgg tgccctcact ggccttcaac gtgtacgtga aatgtcactc
     4981 aacgacggtc acctattcgt tactccagaa caaatccaag aagaattcca acgtgccctt
     5041 cagttgatta tcgatgttta tgaagacttc aacttgactg actaccgctt ccgcctctct
     5101 cttcgtgacc ctcaagatac tcataagtac tttgataacg atgagatgtg ggaaaatgcc
     5161 caaaccatgc ttcgtgcagc tcttgatgaa atgggcgtgg actactttga agccgaaggt
     5221 gaagcagcct tctacggacc aaaattggat atccagatta aaactgccct tggaaaagaa
     5281 gaaacccttt ctactatcca acttgatttc ttgttgccag aacgcttcga cctcaaatac
     5341 atcggagctg atggcgaaga tcaccgtcca gtcatgatcc accgtggggt tatctcaact
     5401 atggaacgct tcacagctat cttgattgag aactacaagg gggccttccc aacatggctg
     5461 gcaccacacc aagtaaccct catcccagta tctaacgaaa aacacgtgga ctacgcttgg
     5521 gaagtggcca agaaactccg tgaccgcggt gtccgtgcag acgtagatga gcgcaatgaa
     5581 aaaatgcagt tcaagattcg tgcttcacaa accagcaaga ttccttacca attaattgtt
     5641 ggagacaaag aaatggaaga cgaaacagtc aacgttcgtc gctacggcca aaaagaaaca
     5701 caaactgtct cagttgataa ttttgttcaa gctatcctag ctgatatcgc caacaaatca
     5761 cgcgttgaga aataagagtc tagcataaaa gcctccaatc tggaggcttt ttctcatcta
     5821 tttttactca aggactaagt tcacttgagc aaactgaatc cgcactgtcg ttccttttcc
     5881 gacctcagac tcgatacgaa tctggtgccc cagttcttca gaaattttct tagatagata
     5941 aaggccaagt ccagaggact gctggatcaa acggccattg tatcctgaaa agccacgttc
     6001 aaatactcgg aggacatcac tgttttttat cccgattccc gtatctttga tacaaagctc
     6061 ttggtcatcc atataaatct ccagaccacc ttccttggtg tacttgagac tgtttgagat
     6121 gatttgctca ataaccacta gcagccactt tttatccgtc acgatttctt tatcaaggtc
     6181 atgtagattg acatttaagc ctttttgaat aaagaaaaga gcatatttac gaattatttc
     6241 cttgaccaag tcctcaattt gaacctgctt taagaccaaa tcatcatgga aactttctaa
     6301 acgcaggtac tgtaaaacta ggttggtata ggagtcgatt ttgaaaattt cctgttctag
     6361 ttgctgcttc agttggcggt cgaccacttc tgcaactaag agttgactgg ctgcaatggg
     6421 ggtctttatc tgatggaccc acaaggtata gtaatccagc aaatccgtca gttttctttc
     6481 tgcttttgac ctctgctgat agagttccat ctcacgcgct tctaattttt ctgctaaagc
     6541 tatttccaaa ggagacttgg cttccctctc tccatagaga agttcctggc gatagacctg
     6601 cgtttccacc aatatgtccc aagtgaaaaa taatatggtt acaaagcaac acaagaagaa
     6661 aaagtagagg aagtaaattc ctagactggc aaataaaaac tgaaagagta agacaagaaa
     6721 tgccaaagaa agcagataga taaaaagacg actacgggag cgcagatagg ctagaaaaaa
     6781 ttgtttccaa tcaagcatgc ttcaatccgt accctattcc tttcttggtc tcgataaatc
     6841 ctaccaatcc ctgctcctcc aactttttac gcaaacgagc cacattgaca gagagggtat
     6901 tatcatcaat gaaaaagtca ctgttccaaa gttcccgcat caggtcgtca cgtgctacga
     6961 tgttgcctgc atgctcaaat aacacgcgta aaatctggaa ttcattcttg gtcaaattca
     7021 agacttgccc ttgataatgt aaatccatgg atttggtatt gaggataaca ccagcatatt
     7081 ccagcaaact ctcatcacgc ccaaactcat aggaacgacg caacaagccc tgaaccttag
     7141 ctaaaagaac ctgctggtca aaaggcttgg tcacaaagtc atccgccccc atattgattg
     7201 ccatgacaat atccatagcc tggtctctcg aagaaagaaa catgataggt accttggaaa
     7261 tcttgcggat ttcctgacac cagtgataac cattaaacaa gggcaaacca atatccatga
     7321 ggaccagatg aggttccgac tgaacaaata gactcaaaac ttccataaag tcttctacca
     7381 ggaccacttc aaatccccat tcagagagca ttttcccaat ctgttgacga atgacctgat
     7441 catcttctat taataaaatc ttgtgcatgc gcttctcctt ttccattatt ataacagatt
     7501 tttccatgct agatggtctg aaactgaatt tgaaatagcc tgtttttagc cagtacaaac
     7561 aggctatgct actagctaat ttgagggaaa tttgctaaga taaataaaaa gaaaggagct
     7621 cttatggcca atatttttga ctatctgaaa gatgtcgcat atgattctta ttacgacctt
     7681 cccttgaatg agttagacat tctaacctta atagaaatca cctacctctc ctttgataat
     7741 ctggtctcca cacttcctca acgtctttta gatctagcac ctcaggttcc aagagagccc
     7801 accatgctta ctagcaaaaa tcgccttcaa ttattagatg aattggctca acacaagcgc
     7861 ttcaaaaatt gcaaactctc ccattttatc aacgacatcg accctgaact gcaaaagcaa
     7921 tttgcggcta tgacttatcg tgtcagcctc gatacctatc tgattgtctt tcgtgggaca
     7981 gatgacagta tcattggctg gaaggaagat ttccacctga cctatatgaa ggaaattcct
     8041 gctcaaaagc acgcccttcg ctatttaaag aacttttttg cccatcatcc taagcaaaag
     8101 gttattctgg ctgggcattc caagggagga aatctcgcta tctatgctgc tagccaaatt
     8161 gagcaaagtt tgcaaaatca gatcacagca gtttatacat ttgatgcacc tggtctccat
     8221 caagaattga cacagactgc gggttatcaa aggataatgg atagaagcaa gatattcatt
     8281 ccacaaggtt ccattatcgg tatgatgctg gaaattcctg ctcaccaaat catcgttcag
     8341 agtactgccc tgggtggcat cgcccagcac gataccttta gttggcagat tgaggacaag
     8401 cacttcgttc aactggataa gaccaacagt gatagccggc aagtagacac aacctttaaa
     8461 gaatgggtgg ccacagtccc tgacgaagaa cttcagctct acttcgacct cttctttggc
     8521 actattcttg atgctggtat tagctctatc aatgacttgg cttccttaaa ggcgcttgaa
     8581 tacattcatc atctctttgt ccaagctcaa tccctcactc cagaagaaag agaaaccttg
     8641 ggtcgcctta cccagttatt gattgatact cgttaccatg catggaaaaa tagataatac
     8701 tcttgaaaat taaatgtata caaaacaaaa gacctagaat acatactttc atgtgcattc
     8761 taagtctttt taaatagaat ctaatagtca ataaaaatca aagagcattg agagataatg
     8821 gggcttggaa cgtccctctc gtttcaacaa aatgacccca ttatagatta aaaagatgcc
     8881 acttagaaaa agcaaaaaag gaagtaagac aaaggcaaat acataaaaag ctaactgaac
     8941 attctcgtat ccatttttat aaaaaaggta ggatagataa aaataacttg aaatgaggga
     9001 taataaaaat aatactggat tccacaaact tctattatcc ttccaaaatg acactataaa
     9061 ggctaataca atttctataa cgagatacat ttcttactcc tttaatagct acattttatc
     9121 ataattatcc aaagaaaaaa agagggcatt tatccctctt aatccttcat ctgactctct
     9181 gcatcggcca cgactttttc tagactggtc tgaccaagtt ctgcctccat agtcaactga
     9241 attctctcca atttttgatc caaaacatca tgaatatgag ctcctacagg gcaatttgga
     9301 ttcggattgt catggaaact gaagagttga cctgtcttac caagacattc gaccgcctga
     9361 taaacatcta aaagactaat atccttaagg tccttgacaa tctctgttcc gcccgttcca
     9421 cgcgctactg aaatcagctc tgccttcttc aactgggaca agatctttct gataatgaca
     9481 ggattgaccc caacactagc agccagaaaa tcactggtca ccttgctttc cttcccctcg
     9541 agggcaatga ttatcagcat atgagttgca atggtaaatc tacttggaat ttgcatcctc
     9601 ttctcctttt tacgaggcta ccctgcctct actcttcttt ttctattatt ataccctttt
     9661 tagttgtaat gtcaatcgtt accacttttc aaccagtcgt ctaactcccg atcgcaaccc
     9721 tctttctgag ccaattctct caaaaattcc tgatgatgag tatggtggat cccattgacc
     9781 agactttcat agtaaacctc aaaataggga agtctcaggt ctttagccag ctgcaattca
     9841 gctgccacat cgtaatctac ccgtcggaag tccatatcta ccaggccctt gtcatcaaac
     9901 tccaaaatca tatactgggc ccgcaagtcc ttccgtagct gagcgtccaa aaagaaaggt
     9961 tggccaatcg aacccggatt gacaatcaat tgcccaccag tcccgtaacg aagcaactgc
    10021 tggtgaatat gtccataaac agcaatatca cagggaggat gagtcaccaa gcggtcaaac
    10081 tcctcttgtt tgccagtatg aatcaactct cgcccccagt tcttatcagg cagatgatgg
    10141 ctaattccca ccgtcaaatc cccaaactga cgatgaattt gaagaggttg attgtggagc
    10201 acttcaattt cttctaggga aatttcctct aaaacatact ggcactggcg caagagatag
    10261 cgttgactag ggcgagtact atccaattcc ttacggacac catgccaaag actgtcttcc
    10321 cagtttccca aaactctagc cgtaatcggt agttgatcca acaagtccaa aatccttcta
    10381 cgtcctgtcc ctggcatgag aatatctccc aaaagccagt attcatccac tcctagctgc
    10441 tgagcatctg ccaaaacagc ctccaaggcg gtggtatttc catgaatatc tgaaagaaga
    10501 gctattttcg tcatatccat ctcctcgttt tttctcttgc aataagtata acataaaaag
    10561 tcacagctag agaaatctag ctttttttga tatactagat aaagatatta gacaagagga
    10621 aacgaatgac cccaaacaaa gaagactatc taaaatgtat ttatgaaatt ggcatagacc
    10681 tgcataagat taccaacaag gaaattgcgg ctcgcatgca agtctctccc cctgccgtaa
    10741 ctgaaatgat caaacgaatg aaaagtgaaa atctcatcct aaaggacaag gaatgtggct
    10801 atctactgac tgacctcggt ctcaaactgg tctctgagct ctatcgtaag caccgcttga
    10861 ttgaagtttt tctagttcat catttagact atacaagtga ccagattcac gaggaagctg
    10921 aggtcttgga acacactgtc tctgacctgt tcgtggaaag actagataaa ctgctaggtt
    10981 tccctaaaac ctgcccccac ggaggaacta ttcctgccaa gggagaactc ctcgttgaaa
    11041 tcaataacct cccactagct gatatcaagg aagctggcgc ctaccgcctg actcgggtgc
    11101 acgatagttt tgacattctc cattatctgg acaagcactc acttcacatc ggtgaccagc
    11161 tccaagtcaa gcagtttgat ggcttcagca ataccttcac tatcctcagt aacgacgagg
    11221 atttacaagt gaatatggac attgcaaaac aactctatgt cgagaaaatc aactaatttc
    11281 tcaagtcccc taccaaccct gaaagtttta ttttcagggt tttcttatct attttttata
    11341 tcaaaaacga tagtaaacgt tttcatatcc cgactgaata ctgtataata attatataaa
    11401 gcattacgtc actaacagat gaaaaaagga ggttttagca tgaaaaaaat catcttcatc
    11461 aaaaccattc aactccttgt cattgatgga atcatgctgg catttttgac atttaaaagg
    11521 ggggcttact tgggactgga ttttgattta tagtggttgg ctcattttct ttcatcctgt
    11581 gctattgacc tatctttcaa accaactttg tgaccacttt agttaactct attcccagat
    11641 tagaccgaga ttctggcgtt ttgctttaca aattctccta tgggatagcc tgatgattct
    11701 ctccttggtg tctttaagtg atattccact tttccttcag ggaactctcc tcatcctagg
    11761 atatctcatc ccttcctatc gcatctgcca aagcctgaaa agagacttcc cccaagcata
    11821 tcaagaaccg atttcttttt ggagtatttt atgatcgatg agaaagacca agccgactgg
    11881 gcttggtctt tcttatctct ttttagtatc taggataatg gtaacaggtc cgttattaac
    11941 cagttcaacc tgcatatctg ctccaaagat gcctgtctga acgggcactt cttgcgctaa
    12001 tttttgattg aaagcatcat agaagtctga tgccatatca ggtttagctg cccctgtaaa
    12061 ggctggacga ttgcctttct tagtatccgc aaagagggta aactgagaaa tagagaggat
    12121 ttctccttca atatctttga cagacaggtt catcttgcct tctgcgtctg aaaaaatccg
    12181 catattgacc agttttctca cagcatagtc caaatcttcc tcttggtcct ctggtccaac
    12241 accaaccagc aataaaagtc cctgattgat ttttccctga atctggcctt ctatactcac
    12301 ttgggctttt ttaacccgtt ggataatgat tttcataata gcctttctag taagagctag
    12361 gacaactagc cgttggtccg tttgacagag taaacttctg gcacactctt aattttatcg
    12421 acaaccgtgg tcagtgtaga gaggttggca ataccgaagg acacatggat attagcaaac
    12481 ttcatatcct tggttggttg ggcattgacc gttgaaatat tcttggttgt atttgaaaga
    12541 acttgcagta catcgttcaa cagtcctgta cggttgagac cgtagatatc gatatgggcc
    12601 atatactcct tatttgagct agagtactgg tcttcccatt ccacatcaag gagacgttgc
    12661 tcgtagtttt cttgggcacg caggttcata cagtccacac ggtgaatagc cacaccacga
    12721 cccttggtaa tgtagccaac aatatcgtca ccaggcacgg ggttacaaca cttagcaatc
    12781 cgcactagga gaccagaagc accttcaata accactcccc cctcatgctt gaccttgaga
    12841 gtttctttat tttcaacctt gacctcgcca cctttgacaa gctcctctgc ctcagccttg
    12901 gccttggcac gctcttcctc acggcgttcc ttttcagtca gacgattaaa gacggtaatc
    12961 gcaccgattt ccccaaaacc aatggccgca aagagggagt cttctgtctt gtaactggtc
    13021 ttttgcagaa cttgatccat gtggcgcttg tccataaatt tatttgccac atagccattt
    13081 tcttggaact gagccatcag catctcacga cccttgttga cagacaattc cttatcttgg
    13141 tttttaaaga actggcgaat cttattgcgc gccttgctag tcttgaccat attgagccag
    13201 tcacggctag gtccaaagga gttcgggttg gcgataattt caacctgatc ccctgtcttt
    13261 aacttggttg tcagtggaac catgcggcca ttgaccttgg caccagttgc tttttcaccg
    13321 accttggtat ggatttcgta ggcaaaatca atcggtcctg aatctttggg aagggaacgg
    13381 acagctccat ctggggtaaa aacgtaaatc tcctcagcca aatagttttc cttaacagag
    13441 tccacaaatt ccttagcatc atcagcctgg tcttggagct ccatcatctc cttgatccag
    13501 ttcattccaa tagctgattc cttgctgtta acttgcccct ttataccttt cttataagcc
    13561 cagtgagccg caaccccgta ctcagccacc tcgtgcattt ccttggttcg aatctggaat
    13621 tcaatcggcc cttttggtcc ataaacagtc gtatggatag actgataacc attggccttg
    13681 cggttggcaa tatagtcttt gaagcgacct ggcatcggtt tccaaaattc atgcacgtaa
    13741 ccaagcatgg cataaacatc actttgggta tctaaaatac aacgaatagc aatcagatca
    13801 tagatttcct caaaccgttt tctcttgtcc tgcattttgc ggaaaattga gtaaatatgc
    13861 ttgggacgac cataaatctt ccctttcaag tgacgttctg tcgtatactc ctctaatttt
    13921 gtgactacct catccaccaa ggcctcacgc tccctgcgct tttccttcat catatgggta
    13981 atcttgtaaa actccgttgg attgagataa cggaaagaca agtcttctaa ttcccatttg
    14041 acactggaaa tccccaaacg atgggcaagt ggggcataga tttccatggt ttctttggaa
    14101 atacgctcct gcttgtcttt tcgaagatgt ttcagggtcc gcatattgtg caagcggtca
    14161 gacagtttga ccaaaataac gcggatgtcc tcagacatgg ccatgagcat cttgcgatga
    14221 ttttccgcta attgctcctc gatcgatttg tactcgacct tgccaagctt ggtaactccg
    14281 tcaacaatca cccgcacatc aggaccaaac tctctttcca aatcgtccaa ggtcgcatct
    14341 gtatcttcca ccacatcatg caagaatcca caagctactg ttacagcatc cagctttagc
    14401 ttagctaaaa tacctgccac ttggataggg tgaatgatat aaggctcgcc tgatttgcga
    14461 tattgaccac tgtggcattc aacagcatag accaaggcct tatggacaaa atgaacatcc
    14521 tcttccgtta aatattcttt ggttaaagcg acaacttctt cgcctgttaa attcacttct
    14581 ttcggcatct ctactctcca attcttccta ccattttatc acttttttaa gaatatgaaa
    14641 actagattgg aacagaataa gaaaaaaata attcaaaatt gcttgataat tctgaattat
    14701 tggcccataa tatactacga agttagattt taaacttagg tgatagaagg agagatagaa
    14761 gaacggaaac catattgtaa cccaaagact ttctgatttc cccaattcca ttgaagatac
    14821 gaaagataaa cggtggaact cgtatcacat acactggtac cttgactgga ttttggaatt
    14881 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc tcaaagcact
    14941 gctttgaggt tgtagataag actgacaaag tcagtcacat atatacggca aggcgacgtt
    15001 gacgcggttt gaagagattg ttgacgcggt ttgaagagat ttttgaagag tataaaaatc
    15061 ctcaagatac tttcttctat cctttagttt ataaggagaa tacctatgaa aaaaactgct
    15121 atttctatct ttgctctcct aatgttagga gtttgctgcc tgttcctatt cagccagcaa
    15181 agctataaaa aactcgttca atactatgct aacgaccaga acctgcccag taggataact
    15241 tatagtgaat atagcgacaa atgataagcc aactacggta gcactctaaa catcacgtct
    15301 atcaaacaag ctaatgacgg agtttatgca acctatgaag ggcaattgac acctttccaa
    15361 tattgataaa ttgataacca gcctgtcttc atctagtcat gctggttttt aagttcattt
    15421 taaatcctta cctattctcc ctaactgtgc tatacttaat ttatactcaa tgaaaatcaa
    15481 agagcaaact agaaagctag ccgcaggctg ttcaaagcac tgctttgagg ttgcagataa
    15541 agttgacgcg gtttgaagag attttcgaag agtattagta cattctttga gattggagct
    15601 agtatgaaaa tccataaaac cgtgaatcct gttgcctatg aaaataccta ttatctagaa
    15661 ggcgaaaagc acctcatcgt cgtcgatcct ggtagtcatt gggaagccat tcgtcagaca
    15721 atcgagaaga tcaacaaacc gatctgtgct attctcttga cccacgccca ttatgaccat
    15781 atcatgagtc tggacttggt tcgcgagacg tttggcaatc ctcctgtcta tatcgcagag
    15841 agcgaagcca gctggctcta cactcctgtc gataatctct ccggtctccc tcgccacgat
    15901 gatatggcag atgtggtcac aaaacctgca gaacacacct ttgtctttca cgaagaatac
    15961 caactagagg aatttcgttt taaggttcta ccgaccccag ggcactctat cggtggtgtt
    16021 tccctagtct ttcctgatgc tcacctagtc ttgacgggag atgctctatt ccgcgaaact
    16081 atcggacgga ccgaccttcc gactggtagc atggagcaac tccttcatag tatccagacc
    16141 caactcttca ccctaccaaa ctacgatgtc tatccaggac atggtccagc tactactatc
    16201 gctcacgaaa aggccttcaa tccctttttc tagcaagatg atgacaatcg aaatttaagt
    16261 aaactatcca gcaaatcttt ctattacaaa aggcatccta tcaaggtttt cacacgtgat
    16321 tggatgcctt ttttctgatg actagatttt ttgtattacc aaataatcac gcgctcctct
    16381 ggtgaacgcc acattccgtc tccttctttg acatcatagg ttgtaaagaa atcgtcgaag
    16441 tttggtactt gcacattgac acggagtttg gctggtgcgt gcacatcgac gctagccaaa
    16501 agtttcataa attctggtcg acctttcatg cgccagatgc gaccgaagtt gtagaagaac
    16561 tcttctgctg agaagtctgc ttctctctta gctgcttcaa gcgctgctgc gattcctccc
    16621 aagtcagcca cgttttctga tacagtcaat ttaccgttaa tggttgctcc ataagaatcc
    16681 tgtccatcaa attggtcaat gactttttgt gttttctcct tgaaggcagc atagtcgctc
    16741 tctgtccacc aatccttgag gctaccattt tcgtcaaagg aagccccgtt agtatcaaag
    16801 gcgtgggaaa tttcatgggc aatcactgcc ccaataccac cgtagttagc agaagatgac
    16861 tgatgcaagt catagaaagg cgcctgtaaa atggccgctg gaaagacaat caggttcttc
    16921 tgaggattgt agtaggcatt gaccatatga gcaggcatgc cccattcctt atagtctaca
    16981 ggctggttcc acttactcca actgtgcttg atttccacac gcgcaaaggc tagagcattc
    17041 tcaaaaagac tggcagtttc attcactacc ttatccttgt aacgtgcagg caattcttct
    17101 ggatagccaa tataaggttt gatcacattg agcttcacga tagcctgttt acaggtttct
    17161 ggagtgagcc agtcattctt aagcagacgc tccttataga catcaatcat ggttgccact
    17221 tttttctcca catccgcctt ggcttctgga gagaacttct cacgggcgta ccaaagaccc
    17281 agggcttgct tgaaaggttc ttgtgctaga tgataagctg ctttgacctt atcttttgcc
    17341 tctggaactc cagaaagggc acggctgtag gcaccagaca aaacacggat atcctctgtt
    17401 aaatagctgg ttgaaagatt gacaacactc aaaatcaagg ttgctttaag gagagaccag
    17461 gcttcctcac tgtagaattg ctctgctgct tgccagaaac gttcctcgtc tacaataacc
    17521 ttgtctggta attgcccaat aactgctttg aagaagtcat ccaaaggtag ggcaggcgcg
    17581 aatttcttaa aatcttcgta agaatatgga tgatagagtt tagcatattc tgaactttct
    17641 tcattagaga gcaccactgc cgcaactcgg cggtccaatt caagtctttt ttctagcaag
    17701 tcttcaattt cttcatcaga gaaatcataa gccttgagga gatttgcgct gctttctttc
    17761 caaagagtca agagctcttc gcgctgagga tgttcttctg catagtaggt cgtatctggc
    17821 aagattgtgc ttggagcgct agcccataga acattgattc tagcatccat aaagtctggc
    17881 gatacaccaa aaggaaggaa gtttggtttt cctgcaagct caaactctgc tagtttagct
    17941 gtaaaatccg caaaagtttc caattcttgg aattctttaa ggagtggtaa gacaggtgtg
    18001 ataccgtcag cttctctctt gtcaaaatca cgaactaggc ggtggtattt gacaaagttt
    18061 tccaagatag catcctcagg cacttcttca cctgctaacc acttgtctgt tgtcgccagc
    18121 atcaggtctt caatttcctg gtctaaatca acaaaacctc cagtttgaga cttatctgct
    18181 gggatttcag ctgtctgttg ccattctcca ttgatagcat cataaaaatc atcttgataa
    18241 cgtgtcatct tgttctcgct ttcatttgta tttgcattta tcttaacaaa aatcactagg
    18301 gaatgcaat
//
LOCUS       DAVLTM010000030        18280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLTM010000030 DAVLTM010000000
VERSION     DAVLTM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.030"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            24..593
                     /locus_tag="VYN16_000905"
     CDS             24..593
                     /locus_tag="VYN16_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403457.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            661..1311
                     /locus_tag="VYN16_000906"
     CDS             661..1311
                     /locus_tag="VYN16_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1403458.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            1295..3535
                     /locus_tag="VYN16_000907"
     CDS             1295..3535
                     /locus_tag="VYN16_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1403459.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLIKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            3714..3902
                     /locus_tag="VYN16_000908"
     CDS             3714..3902
                     /locus_tag="VYN16_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403460.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            3934..4521
                     /locus_tag="VYN16_000909"
     CDS             3934..4521
                     /locus_tag="VYN16_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403461.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            4525..5706
                     /locus_tag="VYN16_000910"
     CDS             4525..5706
                     /locus_tag="VYN16_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403462.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    5866..5997
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6013..6543
                     /gene="infC"
                     /locus_tag="VYN16_000911"
     CDS             6013..6543
                     /gene="infC"
                     /locus_tag="VYN16_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1403463.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            6576..6776
                     /gene="rpmI"
                     /locus_tag="VYN16_000912"
     CDS             6576..6776
                     /gene="rpmI"
                     /locus_tag="VYN16_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1403464.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            6828..7187
                     /gene="rplT"
                     /locus_tag="VYN16_000913"
     CDS             6828..7187
                     /gene="rplT"
                     /locus_tag="VYN16_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1403465.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            7245..7625
                     /locus_tag="VYN16_000914"
     CDS             7245..7625
                     /locus_tag="VYN16_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1403466.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            7875..8675
                     /locus_tag="VYN16_000915"
     CDS             7875..8675
                     /locus_tag="VYN16_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1403467.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            8638..9624
                     /locus_tag="VYN16_000916"
     CDS             8638..9624
                     /locus_tag="VYN16_000916"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1403468.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            9666..11831
                     /gene="lytB"
                     /locus_tag="VYN16_000917"
     CDS             9666..11831
                     /gene="lytB"
                     /locus_tag="VYN16_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1403469.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWIFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWIYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNKNAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLYFDGFKLE
                     NPFLFKDLTEDTNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(11892..13547)
                     /gene="pavA"
                     /locus_tag="VYN16_000918"
     CDS             complement(11892..13547)
                     /gene="pavA"
                     /locus_tag="VYN16_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1403470.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            13652..14149
                     /gene="ybeY"
                     /locus_tag="VYN16_000919"
     CDS             13652..14149
                     /gene="ybeY"
                     /locus_tag="VYN16_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1403471.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            14130..14525
                     /locus_tag="VYN16_000920"
     CDS             14130..14525
                     /locus_tag="VYN16_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1403472.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            14542..15441
                     /gene="era"
                     /locus_tag="VYN16_000921"
     CDS             14542..15441
                     /gene="era"
                     /locus_tag="VYN16_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1403473.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            15489..16313
                     /gene="mutM"
                     /locus_tag="VYN16_000922"
     CDS             15489..16313
                     /gene="mutM"
                     /locus_tag="VYN16_000922"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1403474.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            16313..16918
                     /locus_tag="VYN16_000923"
     CDS             16313..16918
                     /locus_tag="VYN16_000923"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1403475.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            16905..18104
                     /locus_tag="VYN16_000924"
     CDS             16905..18104
                     /locus_tag="VYN16_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1403476.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            18104..18253
                     /gene="rpmG"
                     /locus_tag="VYN16_000925"
     CDS             18104..18253
                     /gene="rpmG"
                     /locus_tag="VYN16_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1403477.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         5376 a         3347 c         4150 g         5407 t
ORIGIN      
        1 aacgtttcaa gcggaggaaa cgtatggaat caatatttgt gaaatttgcc cagtatccgt
       61 ctatagaaac ggagcgttta ttgcttagac ctgtaacttt ggatgatgcg gaagcaatgt
      121 ttgactatgc ctcggacaag ggtaatacac gttacacttt tccaaccaat caaagcttgg
      181 aagaaaccaa gaataacatt gctcagttct atttggctaa tcccttggga cgttggggaa
      241 tagaactaaa aagcaatggt cagtttattg gaaccattga cttgcacaag attgattctg
      301 ttcttaagaa ggcagctatt ggctacatta tcaataaaaa gtattggaat caaggattaa
      361 cgacagaagc caatcgtgct gtgattgagc tagcttttga gaagataggg atgaataagt
      421 tgactgccct tcacgataag gacaatcctg cgtcaggaaa ggtcatggag aaatcaggca
      481 tgcgtttttc ccatgcagaa ccatatgctt gtatggacca gcatgaaaaa gatcgaatcg
      541 ttacaagagt tcattatgtc ttgaccaagg aagactattt tgcaaataaa taagcagttg
      601 aaaagaaatt tttcgactgt tttttcttcc tcttacgaat aatctaagag aggagaaaat
      661 atggaagcaa ttatcgagaa aatcaaagag tataaaatca tcgtcatctg tactggtctg
      721 ggcttgcttg taggcggatt tttcctgcta aagccagctc cacaaacacc tgtaaaggaa
      781 acgaatttgc aggctgaagt tgcagctgtt tccaaggatt tggtatccga aaaggaagtg
      841 aacaaggaag agaaggaaga accagttgaa caagatctaa tcacagtaga tgtcaaaggt
      901 gctgtcaaat cgccagggat ttatgacttg cctgtaggta gtcgagtcaa tgatgctgtt
      961 cagaaggctg gtggcttgac agagcaagca gacagcaagt cgctcaatct agctcagaaa
     1021 gttagtgatg aggctctggt ttacgttcct actaagggag aagaagcagt tagtcaacag
     1081 actggttcgg ggacagcttc ttcaataagc aaggaaaaga aggtcaatct caacaaggcc
     1141 agtctggaag aactcaagca ggtcaaggga ctgggaggaa aacgagctca ggacattatc
     1201 gaccatcgtg aggcaaatgg caagttcaag tcagtagacg agctcaagaa ggtctctggc
     1261 attggtggca aaacaataga aaagcttaaa gactatgtta cagtggatta agaatttctc
     1321 tattccccta atttacctga gttttctatt actttggctt tattacgcta ttttctcagc
     1381 atcttatctt gctttgttgg gctttgtttt tctgctagtc tgtctcttta tccaatttcc
     1441 gtggaaatct gctggtaaag ttctaataat ttgcggaatc tttggatttt ggtttgtttt
     1501 tcaaaattgg caacagagtc aagcgagtca aaatctggcg gattctgttg aaagggtacg
     1561 gattttgcct gatactatta aggttaatgg tgatagtcta tcctttcgtg gcaagtctaa
     1621 cggtcgtgct ttccaagtct attataaact ccagtccgag gaggagaaag aagcctttca
     1681 agctttaacc gacctgcatg agataggact agaagggaag ctttcggagc cagaagggca
     1741 gagaaatttt ggtggcttta attaccaagc ctatctgaag actcagggaa tttaccagac
     1801 tctcaatatc aaaacaatcc agtcacttca aaagattggc agttgggata taggagaaaa
     1861 cttgtccagt ttacgtcgaa aggctgtggt ttggattaag acgcactttc cagaccctat
     1921 gcgcaattac atgacaggac tcttgctggg acatctggac accgactttg aggagatgaa
     1981 tgagctttat tccagtctag gaattatcca cctctttgcc ctatctggta tgcaggtagg
     2041 ttttttcatg aatggattta agaaacttct cttgcgattg ggcttgaccc aagaaaagtt
     2101 gaaatggctg acttatccct tttcccttat ctatgcggga ctaactggat tttcagcatc
     2161 ggttattcgc agtctcttgc aaaagctact ggctcaacat ggggttaagg gcttggataa
     2221 ttttgccttg acggtgcttg tcctctttat tgtcatgcca aactttttct tgacagcagg
     2281 aggagtcttg tcctgcgctt atgcttttat cctgaccatg accagcaaag aaggggaggg
     2341 gctcaaggct gttactagtg aaagtctagt catctccttg ggcatattgc ccattctatc
     2401 cttctatttt gcggaatttc aaccttggtc tatccttttg acctttgtct tttcctttct
     2461 atttgacttg accttcttac cgctcttgtc tattttattt gtcctttcct ttctctatcc
     2521 agtcattcag ctgaacttta tctttgaatg gttggagggc attattcgct tggtgtcaca
     2581 ggtgacaagt agacctctgg tctttggaca acccaatgca tggtttttaa tcctattgtt
     2641 aatttccttg gctttggtct atgatttgag aaaaaacatt aaaaagctaa cggtattgtg
     2701 cttattgatt acagggctct ttctcctgat caagcatcca ctggaaaatg aaatcaccat
     2761 gctggatgtg gggcaaggag aaagtatttt cctacgggat gtaactggga aaaccattct
     2821 catagatgta ggtggtaagg cagaatctta taagaaaatc gaaaaatggc aagaaaagat
     2881 gacgaccagc aatgcccagc gaaccttgat tccctatctc aaaagtcgag gagtagctaa
     2941 gattgaccag ctaattttga ctaacacgga caaggagaat gttggagatt tgtcagaggt
     3001 gaccaaggct ttccatgtag gggagattct agtatcaaaa gacagtctga aacagaagga
     3061 atttgtggca gaactacaga cgactcaaac aaaggtgcgt agtatgacag taggggagaa
     3121 cttgcccatt tttggaagtc agttagaagt tctatctcca aggaaaatgg gagatggagg
     3181 acacgatgac accctagttc tgtatgggaa attcttggat aagcaatttc tcttcacggg
     3241 aaatttggag gagaaaggag agaaggactt gctgaagcac tatccagact tgaaagtaaa
     3301 tgttttgaaa gctagccaac atggcaataa aaaatcatca agtccagcct ttctagaaaa
     3361 actcaaacca gagcttactc ttatctcagt tggaaagagc aatcgaatga aactccccca
     3421 tcaggaaaca ttgacacgac tggaaggtat caatagcaaa gtttatcgaa ctgaccagca
     3481 aggagctata cgttttaagg ggttggatag ttggaaaatc gaaagtgttc gataggaagg
     3541 ataaatgttg tagattagtg aaataaacta aaaatttgtt gcataataat gataaaaatg
     3601 gtataatgaa aacgtattca atattgagga tataaaatca ttaaaaatca gcaaaagttg
     3661 ttttattagt tagtttataa tctattggtc ttcttcagtc cagtgtatct gctgtgacag
     3721 ccactaaaag ttacaagtat gattggaata cggttttgga atatagtacc aactatcacg
     3781 accatcagta tgcttggatt ccggaatggt ctcgttatta cagctattct gagtataaag
     3841 ttggcggagg ctggaactac gctcgttatg aggtcataaa ctactatact ggaggttatt
     3901 aatccttaaa gagtgagaaa aggagggctg gatatgttaa atcttactca tgttacctta
     3961 aaaacgcgac aagtcatctt gcaagatgcg gattttacct ttaaaaaggg taggatttat
     4021 ggccttcttg ctatcaatgg ctcgggaaag acgacactat tccgagctat gagcaagttg
     4081 cttcccctta gtagtggaca catcgcagtt cctccttctt tgttttatta tgagagcgtt
     4141 gaatggctgg atggaaactt aagtgggatg gactaccttc gtctcataaa aaacatctgg
     4201 aagtcagacc taaacttgag agatgaaatc gcctactggg aaatggctga ctatatcagt
     4261 cttcccatcc gcaagtattc cttagggatg aagcaacgct tggtgattgc tatgtatttt
     4321 ctcagtcagg ccaaatgctg gctcatggat gagattacaa atggcttaga cgagtattat
     4381 cgacagaagt tttttgatag gctagcacaa atcgatagac aagaacagct ggttctttta
     4441 agttcccact ataaggaaga gttggttgat atctgcgata gagtagtaac cattcatcag
     4501 gggcagatag aagaggttta gtttatgaaa gatgttagtc tatttttatt gaaaaaagtt
     4561 ttcaaaagtc gtttaaactg gattatctta cttttatttg catctgtact cggtgttacc
     4621 ttttatttaa atagtcagac tgcaaactca cacagcttgg agagcaggtt ggaaagtcgc
     4681 attgcagcca acgagagggc tatcaatgaa aatgaagaga aactctccca aatgtctgat
     4741 accagctcgg aggaatacca gtttgctaaa aataatttag acgtgcaaaa aaatcttttg
     4801 acgcgaaaga cagaaattct aactttacta aaagaagggc gctggaaaga agcctactat
     4861 ctgcagtggc aagatgaaga gaagaattat gaatttgtat caaatgaccc gactgctagc
     4921 tctggcttaa aaatgggggt tgaccgcgaa cggaagattt accaagccct gtatcccttg
     4981 aacataaaag cacatacttt ggagtttccg acccacggga ttgatcagat tgtctggatt
     5041 ttagaggtta tcatcccaag cttgtttgtg gttgcgatta tttttatgct aacacaacta
     5101 tttgcagaaa gatatcaaaa tcatctggac acagctcact tatatcctgt ttcaaaagtg
     5161 acatttgcaa tatcctctct tggagttgga gtgggatatg taactgtgct gtttatcgga
     5221 atctgtggct tttcttttct agtgggaagt ctgataagtg gttttggaca gttagattat
     5281 ccctacccaa tttatagctt agtgaatcaa gaagtaacta ttgggaaaat acaagatgta
     5341 ttatttcctg gcttgctctt agctttctta gcctttatcg tcattgtgga agttgtgtac
     5401 ttgattgctt actttttcaa gcaaaaaatg cctgtcctct ttctttcact cattgggatt
     5461 gttggtttat tgtttggcat ccaaaccatt cagcctcttc aaaggattgc acatctgatt
     5521 ccctttactt acttgcgttc agtggagatt ttatctggaa gattacctaa gcaaattgat
     5581 aatgtcaatc taaattggag catggggatg gtcttgcttc cttgcctgat tatccttttg
     5641 ttagtgggga ttctatttat cgaaagctgg ggaagttcac gtaaaaagga agtttttaaa
     5701 gtttagtttt cctataggga agctaaagaa aaactaacat agggagagaa tcaacttggt
     5761 tctctctttt tgattcgaaa accaaaccaa aatacaaaca caaacttttc aaaaaataac
     5821 tttttatctt gacaagagct agaaaacttg gtatcatata aaagttgaga aaagcagaag
     5881 tgagagcttc tcgccttgtg acattaagtt gcctggccct acggatgaaa agtttcgaag
     5941 aaacgctatc ataacgtgcg ggcttgtata tttacaagtc cgctattgtt tttctctaat
     6001 aaaacaaaag aggtgaaaac catagcaaag caagacttat tcatcaatga tgagattcgt
     6061 gtacgtgaag ttcgcttgat tggtcttgaa ggagaacagc taggtatcaa gccactcagt
     6121 gaagcgcaag ctttggctga taacgctaat gttgacctag tattgattca accccaagcc
     6181 aaaccgcctg ttgcaaaaat tatggactac ggtaagttca aatttgagta ccagaagaag
     6241 caaaaagaac aacgtaaaaa acaaagcgtt gttactgtga aagaagttcg tctaagtcca
     6301 acaattgaca agggtgactt tgatacaaaa cttcgtaatg cacgtaagtt ccttgaaaaa
     6361 ggaaataaag ttaaggtatc tattcgcttt aagggtcgta tgattaccca taaagagatt
     6421 ggtgcaaaag ttttagccga gtttgctgaa gcaactcaag atattgccat catcgaacaa
     6481 cgtgctaaaa tggatggacg tcaaatgttc atgcaattgg cgccagcgac tgacaaaaaa
     6541 taattgtcag aaagttaaat aaaggagaaa aaatcatgcc aaaacaaaaa acacaccgcg
     6601 catcagctaa acgtttcaaa cgtacaggtt ctggtggact taaacgtttc cgtgcttaca
     6661 cttctcaccg tttccacgga aaaactaaga aacaacgtcg tcatcttcgt aaagcatcta
     6721 tggtgcattc aggagattac aaacgtatca aagcaatgct tactcgcttg aaataatagc
     6781 ttgactgtaa gtaaacaatt ctaggaaata tttggaggaa ataaaacatg gcacgtgtta
     6841 aaggtggcgt tgtatcacgc aaacgtcgta aacgtattct taaattagca aaaggttact
     6901 atggagctaa acacatcttg ttccgtactg caaaagaaca agtaatgaac tcttactact
     6961 atgcataccg tgaccgtcgt cagaaaaaac gtgacttccg caaattgtgg attactcgta
     7021 tcaatgcggc agctcgtatg aacggacttt catactcaca attgatgcat ggtttgaaat
     7081 tggctgagat tgaagttaac cgtaaaatgc ttgctgactt ggctgttaac gatgcagtag
     7141 ctttcacagc tcttgcagat gcagctaaag caaaacttgg taaataataa ctgatgagac
     7201 tggaacagga ttgtccagtc tttttaaaaa taaaggagaa agatatggct tcaaaaatgc
     7261 tacacacttg cttacgagta gaaaatcttg aaaaatcaat cgcattttat caagatgctt
     7321 ttggttttaa agaattgcgt cgcagagatt ttccagacca tgccttcacg attgtctatc
     7381 taggtcttga gggtgatgat tatgagttgg agttgactta taactacgat cacggtccgt
     7441 atgtggttgg tgatgggttt gcccatatcg ccctcagtac acctgatctt gaggcacttc
     7501 atcaagaaca cagtgcaaaa ggctatgaag tgactgagcc aaatggtcta ccaggaacta
     7561 caccaaacta ttactttgtt aaagaccctg atggatataa ggtcgaagtc attcgtgaaa
     7621 aataatttcg tgcaagttaa gtagaatatt cgttttctac ttgacttttt ttagctgaaa
     7681 gagtatacta ataaaaattc aacctttaag gggagtccag agagactcac aaggtgtcag
     7741 ataaaagaat agtgcaattt tctagaggag accttttgag tgtgctctct tgtgttgtac
     7801 gattttaact gaggccttgc actagcaagg tcttttcttt atctggtccc cttaaaattt
     7861 aaggaggaaa agttatgaat ctcacatgta agaagcgttt gggtgtcatt cggttggaaa
     7921 ccatgaaggt ggttgcacaa gaggaaatcg cgccagcaat ctttgaatta gtcctagaag
     7981 gagaaatggt tgaagccatg cgagcaggcc aatttcttca tctgcgtgta ccagacgatg
     8041 cccatctctt acgtcgtcct atttcaattt cgtctattga caaggcaaac aagcagtgtc
     8101 acctcattta tcggattgag ggagctggga ctgcaatttt ttcaacctta agtcagggag
     8161 acactcttga tgtgatgggg cctcagggaa atggttttga cttgtctgac cttgatgagc
     8221 agaatcaggt tctccttgtt ggtggtggga ttggtgttcc acccttgctt gaggtggcca
     8281 aggaattgca tgaacgtgga gtgaaagtag tgacagtcct cggttttgct aataaggatg
     8341 ctgttatttt gaaaacggaa ttggctcagt atggtcaggt ctttgtaacg acagatgatg
     8401 gttcttatgg catcaaggga aatgtttccg ttgttatcaa tgatttagat agtcagtttg
     8461 atgctgttta ctcgtgtggg gctccaggaa tgatgaagta tatcaatcaa acctttgatg
     8521 atcacccaag agcctattta tctctggaat ctcgtatggc ttgtgggatg ggagcttgct
     8581 atgcctgtgt tctaaaagta ccagaaagcg agacggtcag ccaacgcgtc tgtgaagatg
     8641 gtcctgtttt ccgcacagga acagttgtat tataaggaga aaattatgac tacaaatcga
     8701 ttacaagtgt ctctacctgg tttggatttg aaaaatccga ttattccagc atcaggctgt
     8761 tttggctttg gacaagagta tgccaagtac tatgatttaa accttttagg ttctattatg
     8821 atcaaggcga caacccttga accacgtttt gggaacccaa ctccaagagt agcagagacg
     8881 cctgctggta tgctcaatgc aattggcttg caaaatcctg gtttagaggt tgttttggct
     8941 gaaaagctac cttggctgga aagagaatat ccaaatcttc ctattattgc caatgtagct
     9001 ggtttttcaa aacaagagta tgcagctgtt tctcatggga tttccaaggc aactaatgta
     9061 aaagctatcg agctcaatat ttcttgtccc aatgttgacc actgtaatca tggacttttg
     9121 attggtcaag atccagattt ggcttatgat gtggtgaaag cagctgtgga agcctcagaa
     9181 gtgccagttt atgtcaaatt aaccccgagt gtgaccgata tcgttactgt cgcaaaagct
     9241 gcagaagatg cgggagcaag tggcttgacc atgatcaata ctctggttgg aatgcgcttt
     9301 gacctcaaaa ccagaaaacc aatcttggcc aatggaacag gtggaatgtc aggtccagca
     9361 gtctttccag tagccctcaa actcatccgc caagtagccc aaacaacaga cctgcctatc
     9421 attggaatgg ggggagtgga ttcggctgaa gctgccctag aaatgtatct ggctggggca
     9481 tctgctatcg gagttggaac agctaacttt accaatcctt atgcctgccc tgacatcatc
     9541 gaaaatttac caaaagtcat ggataaatac ggtattagca gtctggaaga actccgtcag
     9601 gaagtaaaag agtctctgag gtaaactgca atcaatctgt tcttgatttt ttattagttt
     9661 gtaatatgaa tttaggagaa ttttggtaca ataaaataaa taagaacaga ggaagaaggt
     9721 taatgaagaa agtaagattt atttttttag ctctgctatt tttcttagct agtccagagg
     9781 gtgcaatggc tagtgatggt acttggcaag gaaaacagta tctgaaagaa gatggcagtc
     9841 aagcagcgaa tgagtggatt tttgatactc attatcaatc ttggttctat ataaaagcag
     9901 atgctaacta tgctgaaaat gaatggctaa agcaaggtga cgactatttt tacctcaaat
     9961 ctggtggcta tatggccaaa tcagaatggg tagaagacaa gggagccttt tattatcttg
    10021 accaagatgg aaagatgaaa agaaatgctt gggtaggaac ttcctatgtt ggtgcaacag
    10081 gtgccaaagt aatagaagac tggatctatg attctcaata cgatgcttgg ttttatatca
    10141 aagcagatgg acagcacgca gagaaagaat ggctccaaat taaagggaag gactattatt
    10201 tcaaatccgg tggttatcta ctgacaagtc agtggattaa tcaagcttat gtgaatgcta
    10261 gtggtgccaa agtacagcaa ggttggcttt ttgacaaaca ataccaatct tggttttaca
    10321 tcaaagaaaa tggaaactat gctgataaag aatggatttt cgagaatggt cactattatt
    10381 atctaaaatc cggtggctac atggcagcca atgaatggat ttgggataag gaatcttggt
    10441 tttatctcaa atttgatggg aaaatagctg aaaaagaatg ggtctacgat tctcatagtc
    10501 aagcttggta ctacttcaaa tccggtggct acatggcagc caatgaatgg atttgggata
    10561 aggaatcttg gttttacctc aagtttgatg gcaaaatggc tgaaaaagaa tgggtctacg
    10621 attctcatag tcaagcctgg tactacttca aatccggtgg ttacatgaca gccaatgaat
    10681 ggatttggga taaggaatct tggttttatc tcaaatctga tgggaaaata gctgaaaaag
    10741 aatgggtcta cgattctcat agtcaagctt ggtactactt caaatccggt ggttacatga
    10801 cagccaatga atggatttgg gataaggaat cttggtttta cctcaagtct gatggcaaaa
    10861 tggctgaaaa agaatgggtc tacgattctc atagtcaagc ctggtactac ttcaaatctg
    10921 gtggctacat ggcgaaaaat gagacagtag atggttatca gcttggaagc gatggtaaat
    10981 ggcttggagg aaaagctaca aataaaaatg ctgcttacta tcaagtagtg cctgtcacag
    11041 ccaatgttta tgattcagat ggtgaaaagc tttcctatat atcgcaaggt agtgtcgtat
    11101 ggctagataa ggatagaaaa agtgatgaca agcgcttggc tattactatt tctggtttgt
    11161 caggctatat gaaaacagaa gatttacaag cgctagatgc tagtaaggac tttatccctt
    11221 attatgagag tgatggccac cgtttttatc actatgtggc tcagaatgct agtatcccag
    11281 tagcttctca tctttctgat atggaagtag gcaagaaata ttattcggca gatggcctgt
    11341 attttgatgg ttttaagctt gagaatccct tccttttcaa agatttaaca gaggatacaa
    11401 actacagtgc tgaagaattg gataaggtat ttagtttgct aaacattaat aatagccttt
    11461 tggagaacaa gggcgctact tttaaggaag ccgaagaaca ttaccatatc aatgcccttt
    11521 atctccttgc ccatagtgcc ctagaaagta actggggaag aagtaaaatt gccaaagata
    11581 agaataattt ctttggcatt acagcctatg atacgacccc ttacctttct gccaagacat
    11641 ttgatgatgt ggataaggga attttaggtg caaccaagtg gattaaggaa aattatatcg
    11701 ataggggaag aactttcctt ggaaacaagg cttctggtat gaatgtggaa tatgcttcag
    11761 acccttattg gggcgaaaaa attgctagtg tgatgatgaa aatcaatgag aagctaggtg
    11821 gcaaagatta gtactataag tgaatatgat ttgagtgaat agtaagttaa aaatcctgat
    11881 ttcaagtaaa atcaggattt tttcatggat gcaatttttt tggagtctgg tgtgacgcgg
    11941 agggtctttt gtcctgtgta agtgacaaag ccgggttttc caccagttgg tttattgagt
    12001 tttttgactt caatcatatc tacctgcacc agattcgaca ggcgcccttg agagaagtag
    12061 gcagctaact ctgctgcgtc tgtcttgact gcatcagatg ggtcaagatt tcctgagatg
    12121 acaacatggc ttccaggaat gtccttagca tggaaccaaa gttcctcctt gcgggccatt
    12181 ttaaaggtca attcctcatt ttgaagattg tttcgtccga catagatgat ggttttgcca
    12241 tcgcttgcta gatattgttc tagttttttg cgtttctgga ttttctcccg ttgtcttctg
    12301 cggataaaac ctgtttgaat caattcttcg cggatttcag cgatttcttc cagtccagct
    12361 tggttgagga cggtttctac actttccaga tagagaatag tggctttggt ttcttcaatc
    12421 aaatcagtca agtatttgac agcttctttg agtttctgat accgtttaaa atagcgttgg
    12481 gcattctggt tgggagtcag agccttatca agcgcaatca tgataggttg gttggtatag
    12541 tagttgtcta ggataacctg gtcttggtcg ttaggcactt ggtgtaggaa ggttgtcagc
    12601 aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg ccagtaactc tttttcctgt
    12661 tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa cacgacgaat cagttcactg
    12721 gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat agtaggtgtc caacaaatca
    12781 gaaagatttg caaaaggctc tcccacctga tttgcaaaag gaactggact gaaggaagtc
    12841 tcagtcaagc atggcttggt ttcctgattg aaaaaatttc ggaaagcgga aagtttttca
    12901 ctaaccagta tcctttccaa ttcatttgcc gtatcgcgtc ccagaccttg aaagaggctt
    12961 tgaagatttt ttgctgttag ttcttgggtt tgcaggattt caaagagctt ttcatccttg
    13021 atagtaaaag gattgagaga ttctgtactt ggcggagcga tataggtcga tcctggaagt
    13081 aaggtgcggt agctattttg tgaaaagccg acgtgtttga taacttcgag gattttatga
    13141 ctgcttttat cgaccagtag aatattactg tgtttcccca taatttcgat aatcaaggta
    13201 gcctggatat ggtctccaat ctcgttttta ttggaaactg taatttccac aatacggtca
    13261 ttttccactt gctcaatcga ctcaatcagg gccccctgca aatactttct caaaaccatg
    13321 ataaaggtag aaggttgggc tggattttca aaagtcgttt gggtcagctg aatgcgtcca
    13381 aaaactggat gggcagaaag gagcaggcga tggctttggc gattgctgcg gatttgcaag
    13441 accaactctt gttcaaaagg ctgattgatt ttctggatgc gaccattcac taattcgctt
    13501 cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa atgacatcgt tctctccttg
    13561 tgattgtatt ccatagtatt atatcaaaaa ggtagaataa aatcatggaa atgtggtata
    13621 ataaagccaa gtaaagagaa acgagaagca catgtatatt gaaatggtag atgaaactgg
    13681 tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt ttggaatttg cagcccaaaa
    13741 attaggaaaa gaagacaagg agatggcagt cacttttgtg accaatgagc gtagtcatga
    13801 acttaatctg gagtaccgtg acaccgaccg tccgacagat gtcatcagcc ttgagtataa
    13861 accagaattg gaaattgcct ttgacgaaga ggatttgctt gaaaatccag aattggcaga
    13921 gatgatgtct gagtttgatg cctatattgg ggaattgttc atctctatcg ataaggctca
    13981 tgagcaggcc gaagaatatg gtcacagctt tgagcgtgag atgggcttct tggcagtaca
    14041 cggcttttta catattaacg gctatgatca ctatactccg gaagaagaag cggagatgtt
    14101 cggtttacaa gaagaaattt tgacagccta tggactcaca agacaataaa cgaaaatgga
    14161 aaaatcgtga cttgatatcc agtttagaat ttgccatcac agggattttt actgctatca
    14221 aggaagaacg aaatatgcga aaacatgcag tgacggctct agtggtcatc cttgcaggtt
    14281 ttgtttttca ggtgtcacga atcgaatggc tctttcttct attgagtatt ttcttggtag
    14341 tagcctttga gattatcaac tctgctattg aaaatgtggt ggatttggcc agtcactatc
    14401 acttttccat gctggctaaa aatgccaagg atatggcggc cggcgcggta ttagtggttt
    14461 ctcttttcgc agccttaaca ggcgcattga tttttctccc acgaatctgg gatttattat
    14521 tttaaacagt aagaggaaat tatgactttt aaatcaggct ttgtagccat tttaggacgt
    14581 cccaatgttg ggaagtcaac ctttttaaat cacgttatgg ggcaaaagat tgccatcatg
    14641 agtgacaagg cgcagacaac gcgcaataaa atcatgggaa tttacacgac tgataaggag
    14701 caaattgtct ttatcgacac accagggatt cacaaaccta aaacagctct cggagatttc
    14761 atggttgagt ctgcctacag tacccttcgc gaagtggaca ccgttctttt catggtgcct
    14821 gctgatgaag cgcgtggtaa gggggacgat atgattatcg agcgtctcaa ggctgccaag
    14881 gttcctgtga ttttggtggt gaataaaatc gataaggtcc atccagacca gctcttgtct
    14941 cagattgatg acttccgtaa tcaaatggac tttaaggaaa ttgttccaat ctcagccctt
    15001 cagggaaata acgtgtctcg tctagtggat attttgagtg aaaatctgga tgaaggtttc
    15061 caatatttcc cgtctgatca aatcacagac catccagaac gtttcttagt ttcagaaatg
    15121 gttcgcgaga aagtcttgca cctaactcgt gaagagattc cgcattctgt agcagtagtt
    15181 gttgactcta tgaaacgaga cgaagagaca gacaaggttc acatccgtgc aaccatcatg
    15241 gtcgagcgcg atagccaaaa agggattatc atcggtaaag gtggcgctat gcttaagaaa
    15301 atcggtagca tggcccgtcg tgatatcgaa ctcatgctag gagacaaggt cttcctagaa
    15361 acctgggtca aggtcaagaa aaactggcgc gataaaaagc tagatttggc tgactttggc
    15421 tataatgaaa aagaatacta agtagaggtg ggctcatgcc tgcttcttgt tttacagaag
    15481 gaggacttat gcctgaatta cctgaggttg aaaccgtttg tcgtggctta gaaaaattga
    15541 ttataggaaa gaagatttcg agtatagaaa ttcgctaccc caagatgatt aagacggatt
    15601 tggaagagtt tcaaagggaa ttgcctagtc agattatcga gtcaatggga cgtcgtggaa
    15661 aatatttgct tttttatctg acagacaagg tcttgatttc ccatttgcgg atggagggca
    15721 agtattttta ctatccagac caaggacctg aacgcaagca tgcccatgtt ttctttcatt
    15781 ttgaagatgg tggcacgctt gtttatgagg atgttcgcaa gtttggaacc atggaactct
    15841 tggtgcctga ccttttagac gcctacttta tttctaaaaa attaggtcct gaaccaagcg
    15901 aacaagactt tgatttacag gtctttcaat ctgcccttgc caagtccaaa aagcctatca
    15961 aatcccatct cctagaccag accttggtag ctggacttgg caatatctat gtggatgagg
    16021 ttctctggcg agctcaggtt catccagcta gatcttccca gactttgaca gcagaagaag
    16081 cgacagctat tcatgaccaa accattgctg ttttgagcca ggctgttgaa aaaggcggtt
    16141 ccaccattcg gacctatacc aatgcctttg gggaagatgg aagcatgcag gattttcatc
    16201 aggtctatga taagactggt caagaatgtg tacgctgtgg taccatcatt gagaaaattc
    16261 aactaggcgg acgaggcacc cacttttgtc caaactgtca aaggagggac tgatgagaaa
    16321 aatcatcgga atcactgggg gaattgcctc tggtaagtca actgtgacaa attttctaag
    16381 acagcaaggc tttcaagtag tggatgccga cgcagtcgtc caccaactac agaaacctgg
    16441 tggtcgtctg tttgaggctc tagtacagca ctttgggcaa gaaatcattc ttgaaaacgg
    16501 agaactcaat cgccctctcc tagctagtct catcttttca aatcctgaag agcaaaaatg
    16561 gtctaagcaa attcaagggg agattatccg tgaggaactg gctactttga gagaacagtt
    16621 ggctcagaca gaagagattt tcttcatgga tattccccta ctttttgagc aggactacag
    16681 cgattggttt gctgagactt ggttggtcta tgtggaccga gatgcccaag tagaacgctt
    16741 aatgaaaagg gaccagttgt ccaaagatga agctgagtct cgtctggcag cccagtggcc
    16801 tttagaaaaa aagaaagatt tggccagcca ggttcttgat aataatggca atcagaacca
    16861 gcttcttaat caagtgcata tccttcttga gggaggtagg caagatgaca gagattaact
    16921 ggaaggataa tctgcgtatt gcctggtttg gtaattttct gacaggagcc agtatttctt
    16981 tggttgtacc ttttatgccc atcttcgtgg aaaatctagg tgtagggagt cagcaagtcg
    17041 ctttttatgc aggcttagca atttctgtct ctgctatttc cgcggcgctc ttttctccta
    17101 tttggggtat tcttgctgac aaatacggcc gaaaacccat gatgattcgg gcaggtcttg
    17161 ctatgactat cactatggga ggcttggcct ttgtcccaaa tatctattgg ttaatctttc
    17221 ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa tgcaacggca ctgatagcca
    17281 gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac tttgtctaca ggcgtagttg
    17341 caggtactct aactggtccc tttattggtg gctttatcgc agaattattt ggcattcgta
    17401 cagttttctt actggttggt agttttctat ttttagctgc tattttgact atttgcttta
    17461 tcaaggaaga ttttcaacca gtagccaagg aaaaggctat tccaacaaag gaattattta
    17521 cctcggttaa atatccctat cttttgctca atctcttttt aaccagtttt gtcatccaat
    17581 tttcagctca atcgatcggc cctattttgg ctctttatgt acgcgactta gggcagacag
    17641 agaatcttct ttttgtctct ggtttgattg tgtccagtat gggcttttcc agtataatga
    17701 gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa tcatcgtctc ttggttgtcg
    17761 cccagtttta ttcagtcatc atctatctcc tctgtgccaa tgcctctagc ccccttcaac
    17821 taggactcta tcgtttcctc tttggcttgg gaaccggtgc cttgattccc ggggttaatg
    17881 ccctactcag caaaatgact cccaaagccg gcatttcgag ggtctttgcc ttcaatcagg
    17941 tattctttta tctgggaggt gttgttggtc ccatggcagg ttctgcagta gcaggtcaat
    18001 ttggctacca tgctgtcttt tatgcgacaa gcctttgtgt tgcctttagt tgtctcttta
    18061 acctgattca atttcgaaca ttattaaaag taaaggaaat ctagtgcgag taaaaattaa
    18121 tctcaaatgc tcctcttgtg acagtatcaa ttacctaacc agtaaaaatt caaaaaccca
    18181 tccagacaag attgaggttt taaagtattg tcccaaggaa agaaaagtaa ccctacatct
    18241 tgaatctaag tagattttat ggtaaaataa tagggatttt
//
LOCUS       DAVLTM010000031        18167 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLTM010000031 DAVLTM010000000
VERSION     DAVLTM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.031"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(16..951)
                     /locus_tag="VYN16_000926"
     CDS             complement(16..951)
                     /locus_tag="VYN16_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1403478.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAGSLFKATAPITRNELIIEIWRTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GFAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(948..1808)
                     /locus_tag="VYN16_000927"
     CDS             complement(948..1808)
                     /locus_tag="VYN16_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1403479.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1916..4078
                     /locus_tag="VYN16_000928"
     CDS             1916..4078
                     /locus_tag="VYN16_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1403480.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIYKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLEHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQFLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKNLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4170..5429
                     /locus_tag="VYN16_000929"
     CDS             4170..5429
                     /locus_tag="VYN16_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1403481.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5443..6309
                     /locus_tag="VYN16_000930"
     CDS             5443..6309
                     /locus_tag="VYN16_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1403482.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6323..7156
                     /locus_tag="VYN16_000931"
     CDS             6323..7156
                     /locus_tag="VYN16_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1403483.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7467..8909
                     /gene="gtfA"
                     /locus_tag="VYN16_000932"
     CDS             7467..8909
                     /gene="gtfA"
                     /locus_tag="VYN16_000932"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1403484.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9047..10240
                     /locus_tag="VYN16_000933"
     CDS             9047..10240
                     /locus_tag="VYN16_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1403485.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10926..12905
                     /locus_tag="VYN16_000934"
     CDS             10926..12905
                     /locus_tag="VYN16_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1403486.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            12972..14468
                     /locus_tag="VYN16_000935"
     CDS             12972..14468
                     /locus_tag="VYN16_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403487.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14468..15394
                     /locus_tag="VYN16_000936"
     CDS             14468..15394
                     /locus_tag="VYN16_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403488.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15403..16470
                     /locus_tag="VYN16_000937"
     CDS             15403..16470
                     /locus_tag="VYN16_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403489.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKEWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16481..17407
                     /locus_tag="VYN16_000938"
     CDS             16481..17407
                     /locus_tag="VYN16_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403490.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(17482..18167)
                     /locus_tag="VYN16_000939"
                     /pseudo
     CDS             complement(17482..18167)
                     /locus_tag="VYN16_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5348 a         3224 c         3827 g         5768 t
ORIGIN      
        1 tataattgtg ttattttact tccaactatt gagagaaatt tcgccactat ttagccagat
       61 ttcttttccg ttatcacatt gaactaaaag ttttccattt tctgagatgt ctttagcaaa
      121 tcccttgtag tctttttgct ctagtgtgaa agtgacttct tttcctagaa tgaatgactg
      181 ttttttgtat aggtataata gctcttctgc tggtgtttcg aagaaagtac gccagatttc
      241 tatgatcaat tcattccttg ttataggagc tgtagcttta aataagctgc cagctttttc
      301 ttttaattcc tgagggaagt ctttaatagt gaaattgata cctactccaa taatgatatc
      361 tgtgactaag ccagtttcta cagaggtcat tgcttcagta aggattcctc caattttatg
      421 attgtttaga tagatatcat tgacccattt tatgtcgaca tctattaaag ttaggttctt
      481 aatggctttg tagacagctc cagctacaag tagtgtgtag gatggtaatt tgtcataggg
      541 gagatttggt ttaagatgga gtgtcatata aataccacct tgtggtgagt agaaggaacg
      601 ttgaaaacgg cctcggcctg ctgtttgata ggaagctaga tagagggtat ttgcttcatg
      661 gcctaaatca attgcttctt ttgcatctag ttgtgttgat tttgtttcgg gtttaaagct
      721 gactttaatt ggaagatttt cttctagaat ctctggaaga ataaggtcac cattcatcag
      781 tttataccct ctatttttga tactatcaat ttcaatgcct tcttgttcta gtcgcttgat
      841 ggctttccaa attgatgttc ggcttaggga tagtttttct gcgatttttt ctccgctgat
      901 atagtcggtt tctttagata ggatttggta gacagcttgg taggatttca taatgttgcc
      961 tttcttacta gttggtattg agagtattct tttcttgtat gacttggagt ctgattaaag
     1021 tattgtttat aagctttcga aaaatggagt ggatctgaaa aacctaccga gtatgcaatt
     1081 accttgatgg actcttgggt attttcgaga agttgtctag ctcggtgcat tcgaacgtag
     1141 agtaggtatt ctttgggtga taaggtatta aattctttga atacgcttga taagtagctt
     1201 ctgtgaacgg atagttcttt tgctaaatct tgaattgtaa gtgattgagg atagtggcta
     1261 tcaattaatc gtttgcattc aagatagagt tggtgggttg atgaaatatt cttttttttc
     1321 tgattgggag caatagttcc cagatgaaac atcagttcat gaagttgtcc catgatatgg
     1381 agttgagcta attcacttga ttttgtaatc tgagcgaagc ggacaatgtc tgagatgagt
     1441 tttgcagtag tctgggtatg acaagtttca gattggatga gataggattg atcagaaatt
     1501 tgagaaagag caaaataatc aggggatttc cctccagtga ttcctaacca gtagtaggcc
     1561 caaggttctt tactatctgc ttgataaaag gttagttcct ctggttttaa tagaaagaaa
     1621 tctccttctt ttaaatcaac aattttaccc ttgtaatgaa attttccttg tcctttagta
     1681 atgtaatgta ggacgtatgt atcacgaatg gctggaccaa aagagtaatt aggtgtgcat
     1741 tcctcatatc cataaaagct tagggcaagg tcgattgttc cagtctggta ttctgaaaaa
     1801 actaacatgt gctacctcct acctaacatt ttaccatatt cttgagacat ttttctattt
     1861 tagaaagcga tttcaggtga taaaatataa tcaataaagt gatgaggtga agtaaatggg
     1921 agttaggata gagaataatc tattttatgt tgagagtaaa aatctaagtt tgattattga
     1981 aaatcgaaat ggctacttac ttttgaaaca tttaggaaag actattaaga actataaagg
     2041 ttccaatagt gtttatgaac gagaccatgc cttttcagga aatccaacag ctactaatcg
     2101 aacctttagt ttagatactc aacgtcaaat ttttggacaa catggcttag gagattttag
     2161 gaaaccaacc atacaggttc agcataatgt aactgaagta acagactttc gatttgtaga
     2221 agcaaagatt ttaaaaggtc agaatggtcc acagggctta ccttctccac atagcatgga
     2281 cgatacagag actcttgtct taatgttaga agattctaag gctcaactta gtctgacttt
     2341 gtattatact acttttaata atgatgcgac tattgctagc tacagtaaat tagataataa
     2401 tagtaatcag gaagttgtca tctataaaga tttttctttt atggctgatt ttccagctgc
     2461 agattacgaa atagtaactc tgcagggtgc ttatgctcgt gaaaagactg ttagacgtca
     2521 acaggtagaa caaggaatct tttcgattag ttcaaaccga ggtgcttctg gtcatgctca
     2581 aacaccagct cttctactat gcgaacaagg agtcacagag gatgctggga atgtgtttgc
     2641 tattcaacta atgtatagtg gcaactttga agcttttgtt caaaaaaatc aattgaatga
     2701 agttcgggtg gctattggca ttaatccaga aaacttttct tggaagttag cccctgagga
     2761 atactttgaa acaccggtag ctttagtgac tcattcagat cagggattaa ctggtattag
     2821 tcatgaaagt cagaattttg tactggagca cattatgcta agtgaatttt ctaaaaaaga
     2881 acgtccaatt ctaatcaata actgggaagc tacttacttt gactttcaga gagaaaaact
     2941 gttagagtta gcagatgaag ctaagaaagt tggcattgaa ctttttgtat tagatgatgg
     3001 ttggtttggc aatcgttttg atgataatcg tgctttaggt gattgggttg ttaatgagga
     3061 aaaactgggt ggaagtctag aaagtctgat ttcagctatc catgaaagag gtttgcagtt
     3121 tggactttgg ttagaacccg aaatgatttc tgtagatagt gatttgtatc gtcaacatcc
     3181 tgactgggct attcaggttc ctggctatga gcatacttat tctcggaatc aattagtact
     3241 taatcttgcc aatcctcagg tagtagaata cttgaaaagt gtcttagatc aattcctatc
     3301 ttatcatgat attgattaca ttaaatggga tatgaaccgc aatatcacta agctagggaa
     3361 tggattaact tatctagaga cacagatgca atctcatcag tacatgctgg ggctttacga
     3421 actcgtttct tatctgacag agaagcacag ccatattctc tttgagtcct gctctggtgg
     3481 tggtggacga aatgatcttg gtatgatgcg ctatttccca caagtctggg ctagtgataa
     3541 tactgatgcc attgcacgtt taccaattca atacggttca tcctatctct atccaaccat
     3601 ttctatgggg gctcatgtgt cagcagtacc gaatcatcag atgggacgaa tgacaccatt
     3661 agaaacacgt ggccttgtag caatgatggg aaatttgggc tatgaacttg atttgacaaa
     3721 tttatcagat gaagagaaag ctacgattgc taatcaggtg aacttgtata aagaattacg
     3781 accagtagtt cagttaggac agcagtatag actaattaat cctgatactg tatccaatga
     3841 agcagctgta caatttaatt acggaaatca aacgattgta acctacgttc gcgttttatc
     3901 tgttgtagag accatggaaa caactttaaa attaaaaaat ttggatgaag agggactata
     3961 taaattacag gaaaatggcg aagtttactc aggtgcagaa ctaatgtatg cgggcctaac
     4021 tgttatctta tcccaaggag attttttgag tagacagtat atttttagaa aactatgata
     4081 tgtactgacc tcaaaagtta gatttattct atctaacttt tgaggtgcag tttgataagg
     4141 gtgtataaat ttataaagga ggctttctaa tggaatggta taaaaaaatc ggacttcttg
     4201 caactacagg tttagctttg tttgggctcg gtgcttgctc caactatggt aaatctgcgg
     4261 atgacacagt gaccatcgag tatttcaacc agaaaaaaga aatgaccaaa accttggaag
     4321 aaatcactcg tgattttgag aaggaaaacc ctaagataaa ggtcaaagtc gtcaatgtac
     4381 caaatgctgg tgaagtattg aagacacgcg ttctcgcagg agatgtgcct gatgtggtca
     4441 atatttaccc acagtccatc gaactgcaag aatgggcaaa agcaggtgtc tttgaagatt
     4501 tgagtaacaa agactacctg aaacgcgtga aaaatggcta cgctgaaaaa tatgctgtaa
     4561 acgaaaaagt ttacaacgtt ccttttacag ctaatgctta tggaatttac tacaacaaag
     4621 ataaattcga agaactgggc ttgaaagttc ctgaaacctg ggatgaattt gaacagttag
     4681 tcaaagatat cgttgctaaa ggacaaacac catttggaat tgcaggtgca gatgcttgga
     4741 cactcaatgg ttacaatcaa ttagcctttg cgacagcaac aggtggagga aaagaagcaa
     4801 atcaatacct tcgttattct caaccaaatg ccattaaatt gtcggatccg attatgaaag
     4861 atgatatcaa ggtcatggac atccttcgca tcaatggctc taagcaaaag aactgggaag
     4921 gtgctggcta taccgatgtt atcggagcct tcgcacgtgg ggatgtcctc atgacaccaa
     4981 atgggtcttg ggcgatcaca gcgattaatg aacaaaaacc gaactttaag attgggacct
     5041 tcatgattcc aggaaaagaa aaaggacaaa gcttaaccgt tggtgcggga gacttggcat
     5101 ggtctatctc agccaccacc aaacatccaa aagaagccaa tgcctttgtg gaatatatga
     5161 cccgtccaga agtcatgcaa aaatactacg atgtggacgg atctccaaca gcgatcgaag
     5221 gggtcaaaca agcaggagaa gattcaccgc ttgctggtat gaccgaatat gcctttacgg
     5281 atcgtcactt ggtctggttg caacaatact ggaccagtga agcagacttc cataccttga
     5341 ccatgaacta tgtcttgacc ggtgataaac aaggcatggt caatgatttg aatgccttct
     5401 ttaacccgat gaaagcggat gtggattagg agtagagaga ctatgaaaaa agtattacaa
     5461 aaatattggg catgggcttt tgtggtcatc cccctcttgt tacaagcaat tttcttctat
     5521 gtgccgatgt ttcaaggagc cttttacagt tttaccaact ggacaggatt gacttacaac
     5581 tacaaatttg tcggcttaaa caactttaag ctcctcttca tggatccaaa attcatgaat
     5641 gcgattggct ttaccgcaat cattacgatt gccatggtgg ttggtgagat tgcactcggg
     5701 atcttcattg cgcgtgtctt gaactctaaa atcaaaggcc aaaccttctt ccgtgcttgg
     5761 ttcttcttcc cagctgtttt atctggtttg acagtggctt tgatcttcaa gcaagtcttc
     5821 aactacggtc ttccagcgat tgggaatgcc cttcatattg aatttctcca aaccagtctt
     5881 ttagggacta agtggggagc aatctttgcg gctgtctttg tccttctttg gcaaggggtg
     5941 gctatgccca tcatcatctt cctagctggt ttgcaatcta ttccaactga gattacagag
     6001 gcagcaagga ttgatggtgc gactagcaag caagttttct ggaacattga attgccttac
     6061 ttgctaccaa gtgtctctat agtctttatc ctagccctaa aaggtgggct gactgccttt
     6121 gaccaagtct ttgccatgac cggtggtggt ccaaacaatg ccacaacctc acttgggctc
     6181 ttggtttata actatgcctt taaaaacaac caattcggtt atgccaatgc cattgccgta
     6241 atcttgttct tcttaattgt agtgatttcg atcatccaat tgagagtatc taagaaattt
     6301 gaaatttaag aggagaagca tgatgaaaca agatgaaaga aaagccctga ttggcaaata
     6361 cattctattg attctaggat cggttctgat tttagtgccg ctccttgcta ccctctttag
     6421 ttccttcaaa cccactaagg atattgtaga taatttcttt ggctttccaa ccaacttcac
     6481 atgggacaac tttagccgtc tcttagctga tgggattgga ggctattatt ggaactctgt
     6541 cgtcatcact gtcttgtctt tacttgcagt aatgatcttt atccctatgg cagcctactc
     6601 catcgctcgc aatatgagta aaagaaaagc ctttaccatc atgtataccc tcttaatcct
     6661 cggaatcttc gtacctttcc aagtcatcat gattccgatt acggttatga tgagtaaact
     6721 cggtttggct aatacctttg gtttgatctt gctctacttg acctatgcga ttccacagac
     6781 cctctttctc tatgttggct atatcaaaat ctcgattcca gaaagtctgg atgaagcagc
     6841 agagatcgat ggggctaatc aatttacaac ctatttccgc atcatcttcc caatgatgaa
     6901 accgatgcat gcgacaacca tgattatcaa tgccctttgg ttctggaatg acttcatgtt
     6961 gccactcctt gtcttgaacc gggattccaa aatgtggact ctgcctttgt tccaatacaa
     7021 ctacgcaggc caatatttca acgactacgg accaagcttt gcctcttacg tggtcggcat
     7081 tatcagtatc accattgtct atctcttctt ccaacgccat atcatttcag gaatgagcaa
     7141 cggggcagtg aagtaatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
     7201 ctgtacttga gtacggtaag gtgaagctga cgtggtttga agagattttc gaatagtata
     7261 agaagagctc atggaatcta tagtctttga cacagaggaa gagttcagct tgttgataat
     7321 tctatgtaga aaacaagaaa gtagtacttt gtttatcaca gtctatatta gtacgagttt
     7381 gatgataaat agagatttaa aatgaatttt atttatgaag ccagatcata tgagacttgg
     7441 cttcaattag aaaaaggaga gtaatgatgc caattcaaaa taaaaccatg ttgattacct
     7501 attctgatag ccttggaaat aatcttaaag acttatatga taatttggaa gagcattttg
     7561 gagatgctat tggaggagtt caccttttac catttttccc atcaacaggt gatcgtggat
     7621 ttgcgccagt tgactacgac gaagtggact cagcttttgg tgattgggag gatgttaagc
     7681 gtttaggtga gagatattat cttatgtttg attttatgat taatcatatt tctcgtcaat
     7741 ccaagtatta taaggactat caagaaaaac atgaagccag tgaatttaaa gatctctttt
     7801 taaactggga taagttttgg ccagaaaacc gtccgacaca gtctgatgta gatttaattt
     7861 acaagcgtaa ggatcgtgca ccaaagcaag agattgtgtt tgaagatggt tcagtggaac
     7921 atttgtggaa tacctttggt gaggagcaga ttgatcttga tgtgaccaaa gaagtaacta
     7981 tggaatttat ccgtaagacc attcagcact tggcaagtaa tgggtgtgat ttgattcgtc
     8041 tagatgcctt tgcttatgca gtgaagaaat tggatactaa tgatttcttt gtggaaccag
     8101 atatttggga tttattggac aaagttcgag atatcgctgc tgagtatggg acagagcttt
     8161 tacctgagat tcatgaacac tattcgattc agtttaaaat agcagaccat gattactatg
     8221 tttatgattt tgctcttcca atggtgacac tttatactct ttacagttcc agaacagagc
     8281 gtttggctaa gtggttaaag atgagcccga tgaagcaatt tacgacgcta gatacccatg
     8341 atgggattgg agtagtagat gtcaaggata tcctgaccga tgaggagatt gactatgctt
     8401 caaatgaact ctataaggtt ggagccaatg tcaaacgtaa gtactctagt gccgagtata
     8461 acaacttaga tatctaccaa atcaattcaa cctactattc agcgcttgga gatgatgatg
     8521 tcaagtattt tctcgctcgt ctaattcaag cttttgcccc aggtattcct caggtttact
     8581 atgtgggtct attagcaggc aagaatgact tgaaattatt agaagaaact aaagaaggtc
     8641 gaaatattaa tcgtcattac tatagcaacg aggaaatagc aaaagaagtg caacgacctg
     8701 ttgtgaaggc ccttctcaat ctattttctt tccgtaaccg ttcagaagcc tttgatctag
     8761 aagggactac tgagatagag acaccaacag ctcacagcat tgtaatcaaa cgtcaaaata
     8821 aagataagtc cgtaacagca gtagcagaaa ttgatttgca aaatcagact tatcgggtaa
     8881 ttgagaatgg agttgaagta acattttgaa gccttgatat ggtggataaa atagggtttt
     8941 tattttggaa aacgtttcct ttgttttcaa attgctaaaa aagtggtaca atataggata
     9001 gcttactatt atctgaatca gctgatttgg agagaaagga ttcattttga aatcaatagg
     9061 ctttattgaa aagctgaagg ggttgtctag taaagagctg attttattgg gaattatcct
     9121 aagtatcttt ttaccctttt atctttttgt agttgtactc tgtttatata ttatcagttt
     9181 gatttttaca ggagacatga aaagtattct tcagaaaatg ggggagcatc cgatgctgct
     9241 tctttttctt agctatagta ctgttatatc cattcttgca caaaattgga tgggtcttgt
     9301 ggcttcagta ggaatgtttc tatttactat tttctttttg cactatcagt cgattttatc
     9361 ccataaattc tttcgattga ttttgcagtt cgtcttgttt ggtagtgtct tgtcagctgc
     9421 ttttgccagt ttagaacatt tccaaattgt gaagaaattt aactatgctt ttctttcacc
     9481 caatatgcag gtgtggcatc agaaccgggc agaagtgacc ttctttaatc ctaattatta
     9541 tggaattatt tgttgtttct gtattatgat tgctttctat ctgtttacaa cgaccaagtt
     9601 gaattggttg aaagtattct gtgtgattgc aggctttgtt aatctctttg gtttgaactt
     9661 tactcaaaat cgaactgcct ttcctgctat tatcgctgga gcaattatct atctctttac
     9721 gactattaaa aactggaagg ccttttggct tagtattggg gtcttcgcga ttggtttgag
     9781 tttcctcttt tctagtgatt tgggagttcg aatgggtact ttagactctt ctatggaaga
     9841 acgcatttct atctgggatg ctgggatggc cttgtttaag caaaatcctt tttggggtga
     9901 agggccattg acctatatgc actcttatcc tcggatacat gctccttatc atgaacatgc
     9961 ccacagtctt tatattgata cgattctgag ttacggaatt gtgggtacca ttttattagt
    10021 tctgtcttct gttgctcctg ttcgcttgat gatggatatg agtcaggagt cggggaaacg
    10081 tccgattatc ggcctttatc tatctttcct tacagtggtt gctgtgcacg gaatttttga
    10141 cttggctctc ttctggattc agtcaggctt tattttcttg ctagttatgt gcagtattcc
    10201 attggagcat cgaatgttgg tatcggacat gacggattaa gttttataag tttgaaatct
    10261 tctaccttgg gtaggagatt ttttatacga tgggcatgtc gtggatctga ggtgtaagtc
    10321 ctctgtaggg atccactacc ggtgaataca atagcgctcc ccaagcctga ctatcgtgag
    10381 gtagcggatt aaaatggtct ggggatagat cgttttaagt ctgacgcagg aaataagaat
    10441 tgtcagagaa agcaatagtg aaatcgtagg ctatgaatag aaacgtgata gcgaggagca
    10501 tatagtggac aaggtagcga gaaattttaa agtataaaag atagtcttaa tctatatgtg
    10561 taaatcacga aagtaattga attcggaata aggttggtgt gaaaagacat agtaaactga
    10621 atcttgaata ctagaatgat acttctacaa tattgttata atttaatttg aattccaaaa
    10681 tcacattgtt ccgtccgtat ttcattttac tgtatgtctt cctaagctcc aaaatttctg
    10741 tgtcaaattt cctattttca ctgtagtctt tgacacttcg aacgacaatt tgaatcatat
    10801 attcaaaaaa aaatgaaaaa tttgtgccaa caagcctaaa tattctgaaa atttgtttga
    10861 tttttaatgg ataatgtgat ataatggttg tcaatctata attcacataa acagaggagt
    10921 agaaaatgaa aaaaaatcgt gtatttgcta cagcaggtct tgttttatta gcagcaggtg
    10981 tacttgcagc atgcagttct tcaaaatcat ctgattcatc agcccctaaa gcttatggct
    11041 atgtttatac agcagaccca gaaaccttgg actacctgat ttcaagtaaa aatagtacaa
    11101 cagtagtgac ttcaaatggg attgatggtt tattcactaa cgataattac ggtaatcttg
    11161 ctcctgcagt tgcagaggat tgggaagtct ctaaggatgg tttgacctac acttataaga
    11221 ttcgtaaagg ggttaaatgg tttacctctg atggagaaga atatgcagag gtgacggcta
    11281 aagatttcgt gaacggttta aaacacgcag cagataaaaa atcagaagct atgtatttag
    11341 ctgaaaattc ggttaaaggc ttggcagatt atctatcagg aacttcaaca gatttttcaa
    11401 cagttggtgt caaggcggtt gatgattata cgttacaata cactttgaac cagcctgaac
    11461 cgttctggaa ctctaagttg acctattcta ttttctggcc tctgaatgaa gaattcgaaa
    11521 catcaaaagg aagcgatttt gctaaaccaa cagatccgac atccttgctt tataatggtc
    11581 cattcttgtt gaaagggttg actgcaaaat cttctgtaga gtttgtaaaa aatgagcaat
    11641 attgggataa agaaaatgtc cacctagata ctatcaatct agcttactat gatggatcag
    11701 atcaggagtc gctagagcgt aacttcacta gtggagctta tagttatgcc cgtctttacc
    11761 ctaccagctc caactattct aaggttgcag aagaatacaa ggacaatatc tattacacac
    11821 aatcaggctc tgggattgct ggtctgggtg tgaatattga tcgccaaagt tacaactata
    11881 cctctaaaac tacagattca gagaaagtag ctactaagaa ggcattgctt aacaaagatt
    11941 tccgtcaagc cttgaatttt gctcttgatc gctcagctta ctcagctcaa atcaatggta
    12001 aagatggagc agctttagca gttcgtaatt tatttgtaaa accagacttt gtttcagctg
    12061 gtgagaagac ctttggtgat ttagtcgctg ctcaacttcc tgcttatggt gatgagtgga
    12121 aaggtgtgaa tttagctgat gggcaggatg gtttattcaa tgctgacaag gccaaggcag
    12181 agtttgcgaa agctaagaaa gctttagaag cagacggcgt tcagtttcct attcatctgg
    12241 acgttccagt agaccaagca tcaaaaaact acatatctcg tattcagtct tttaaacaat
    12301 ctgtagaaac agttcttggt gttgaaaatg tcgttgttga tattcaacaa atgacaagtg
    12361 atgaattcct taatattact tactatgctg ccaatgcttc atctgaggat tgggatgtat
    12421 caggaggagt ttcatggggt ccagactatc aagacccatc tacttacctg gatattttaa
    12481 aaacaactag cagtgaaact acaaaaacat atttaggatt tgataatcca aatagccctt
    12541 cagtagttca agttggtttg aaagaatacg ataaattagt tgatgaagct gccagagaga
    12601 caagcgactt gaatgtccgt tatgaaaaat atgcagcggc tcaagcatgg ttgacagata
    12661 gttcactctt tattcctgct atggcttctt ctggtgcagc accagtgctt tcacgaattg
    12721 ttccatttac tggagcttct gcgcaaacag gctctaaggg gtcagatgtt tacttcaaat
    12781 atttgaaatt acaagataaa gtggtgacta aggaagagta tgaaaaagct cgtgaaaaat
    12841 ggttgaaaga aaaagctgaa tcaaatgaga aagctcaaaa agaattggca agtcatgtga
    12901 agtaaataat ttctaataga actttctctc catgaggaga aggttctttt gggattttta
    12961 aaggaaataa tatgaaaaaa tatattttta tgcgtgtttt gcggtcattg gtttcgattt
    13021 tcttagtaac gactttgacc tacacgatta tctatacctt ggttcctcga aaattgattt
    13081 tcaagcagga tcctaactat aataaaattg cgacaacggc tgataaacgt gataactatg
    13141 aaaatactgt gtttgagcgt atgggctaca ttgagtatta cgatactaaa gagttgcaag
    13201 aaaaggcaag tagcatggat tcttctgtaa cagtagaagc aaatgcgact aataaagcta
    13261 tttatgaaaa gtacatcaat caattaggtc atggttggac tttgggagaa tttactgaaa
    13321 gtggtcaatt ctatgctact cgtgaaattc caatttttga acgtgttttt cacttctatg
    13381 ctaacttgat tgacattgac catacaaata aaatccaaga ccctgaaaat ccagacttga
    13441 aacgctacct tcgttttgaa aatgatccag ctatcggatg gtcattggtc ggttcaggaa
    13501 ctaaacataa atatctcttg tactttaaca gtcagttccc atttgttcat caaaactttg
    13561 tgaacttgaa tttaggtgac tcttacccaa cctatgctaa tacaccagtt cttcaggtta
    13621 ttactcaagg tcaaggacaa accaaaactg cccaagttca gttcccaaca ggtaagaaaa
    13681 cgtcttctgt aaatatttac tcaagaacct acaagtcacc tagtcaggct gactctcgtg
    13741 aagtagctag ctatgggaaa gatgatcctt atacagcgac tgaaagtaat taccaatatc
    13801 catctatgat tgtcagctct gctattactg gtttgattgg tttggttctt gcctatgctc
    13861 ttgccgtgcc acttggttca gccatggctc gtttcaagaa cacttggatt gatagcctct
    13921 caacagggac tttgaccttc ttgcttgctc ttccaacgat tgccttggtt tacatcgttc
    13981 gattgattgg atcatctatt gcccttccag attcattccc tatcttggga gctggagatt
    14041 ggcgttctta cgttttacca gcagtcatcc ttggtttgtt gggtgctcct gttacagcca
    14101 tttggattcg tcgttacatg attgacttgc aatctcaaga ctttgttcgt ttcgctcgtg
    14161 caaaaggttt gtctgaaaaa gaaatttcaa acaaacacat ctttaaaaat gccatggttc
    14221 cgctggtttc aggaattcct ggtgccatta ttggggttat cggtggtgca acccttactg
    14281 aaacagtctt cgccttccca ggtatgggta aaatgttgat tgactctgta aaagcatcta
    14341 ataactctat ggtcgttggt cttgtcttca tctttacatg tatttctatc ttctcacgtc
    14401 ttttgggaga tatttggatg actattattg acccacgtat taaattgact gagaaaggag
    14461 gcaaataatg tctacaatcg ataaagaaaa atttcagttt gtaaaacgtg acgattttgc
    14521 ctctgaaact attgatgcgc cagcatattc ttactggaaa tcagtgttta aacaatttat
    14581 gaagaaaaaa tcaactgtag tcatgttggg aatcttggta gccatcattt tgataagttt
    14641 catctaccca atgttttcta agtttgattt caatgatgtc agcaaggtaa acgactttag
    14701 tgttcgttat atcaagccaa atgcggagca ttggttcggt actgacagta acggtaaatc
    14761 gctctttgac ggtgtctggt tcggagctcg taactccatc ctcatttctg tgattgcgac
    14821 agtgattaac ttggttatcg gtgtttttgt cggtggtatt tggggtattt caaaatcagt
    14881 tgaccgtgtc atgatggaag tttacaacgt catctcaaac atcccacctc ttttgattgt
    14941 tattgtcttg acttactcaa tcggagctgg attctggaat ctgatttttg ccatgagcgt
    15001 aacaacatgg attggtattg ccttcatgat ccgtgtgcaa atcttgcgct atcgtgactt
    15061 ggaatacaac ttggcgtcac gtactttggg aacaccaacc ttgaagattg ttgccaaaaa
    15121 tatcatgcct caattggtat ctgttattgt gacaaccatg actcaaatgc ttccaagctt
    15181 tatctcatac gaagccttct tgtctttctt cggtcttgga ttaccgatta cagtgccaag
    15241 tttgggtcgt ttgatttcgg attattcaca aaacgtaaca accaatgctt acttgttctg
    15301 gattccattg acaacccttg tcttggtatc cttgtccctt ttcgtagttg gtcaaaactt
    15361 agcggatgct agtgatccac gtacacatag ataggagtag aaatgacaaa agaaaaaaat
    15421 gtaattttga ctgctcgcga tattgtcgtg gaatttgacg ttcgtgacaa agtattgaca
    15481 gccattcgcg gcgtttccct tgaactagtt gaaggagaag tattagcctt ggtaggtgag
    15541 tcaggatcag gtaaatctgt tttgacaaag accttcacag gtatgctcga agaaaatggt
    15601 cgtattgccc aaggtagtat tgactaccgt ggtcaggact tgacagcttt atcttctcac
    15661 aaggagtggg aacaaattcg tggtgctaag attgcgacta tcttccagga cccaatgact
    15721 agtttggacc ccattaaaac aattggtagt cagattacag aagttattgt aaaacaccaa
    15781 ggaaaaacag ctaaagaagc gaaagaattg gccattgact acatgaataa ggttggcatt
    15841 ccagacgcag atagacgttt taatgaatac ccattccaat attctggagg aatgcgtcaa
    15901 cgtatcgtta ttgcgattgc ccttgcctgc cgacctgatg tcttgatctg tgatgagcca
    15961 acaactgcct tggatgtaac tattcaagct cagattattg atttgctaaa atctttacaa
    16021 aacgagtatc atttcacaac aatctttatt acccacgacc ttggtgtggt ggcaagtatt
    16081 gcggataagg tagcggttat gtatgcagga gaaatcgttg agtatggaac ggttgaggaa
    16141 gtcttctatg accctcgcca tccatataca tggagtctct tgtctagctt gcctcagctt
    16201 gctgatgata aaggggatct ttactcaatc ccaggaacac ctccgtcact ttatactgac
    16261 ctgaaagggg atgcttttgc cttgcgttct gactacgcaa tgcagattga cttcgaacaa
    16321 aaagctcctc aattctcagt atcagagaca cattgggcta aaacttggct tcttcatgag
    16381 gatgctccaa aagtagaaaa accagctgtg attgcaaatc tccatgataa gatccgtgaa
    16441 aaaatgggat ttgcccatct ggctgactag gaggaaggaa atgtctgaaa aattagtaga
    16501 aatcaaagat ttagaaattt ccttcggtga aggaagtaag aagtttgtcg cggttaaaaa
    16561 tgctaacttc tttatcaaca agggagaaac tttctcgctt gtaggtgagt ccggtagtgg
    16621 gaaaacaact attggtcgtg ctatcatcgg tctaaatgat acaagtaatg gagatatcat
    16681 ttttgatggt caaaagatta atggtaagaa atcgcgtgaa caagctgcgg aattgattcg
    16741 tcgaatccag atgattttcc aagaccctgc cgcaagtttg aatgaacgtg cgactgttga
    16801 ttatattatt tctgaaggtc tttacaatca ccgtttattt aaggatgaag aagaacgtaa
    16861 agagaaagtt caaaatatta tccgtgaagt aggtcttctt gctgagcact tgactcgtta
    16921 ccctcatgaa ttctcaggcg gtcaacgtca acgtatcggt attgcccgtg ccttggtcat
    16981 gcaaccagac tttgttattg cagatgagcc aatttcagcc ttggacgttt ctgtacgtgc
    17041 ccaagtcttg aacttgctca aaaaattcca aaaagagctc ggcctgacct atctcttcat
    17101 cgcccatgac ttgtcggttg ttcgctttat ttcagatcgt atcgcagtta tttacaaggg
    17161 tgttattgta gaggttgcag aaacagaaga attgtttaac aatccaattc acccatatac
    17221 tcaagccttg ctttcagcgg taccaatccc agatccaatc ttggaacgta agaaggtctt
    17281 gaaggtttac gacccaagtc aacacgacta tgagactgat aagccgtcta tggtagaaat
    17341 ccgtccaggt cactatgttt gggcgaacca aaccgaattg gcacgttatc aaaaaggact
    17401 aaactagtaa tggttttata atttccatgt caatttttat ggaaattagg ctctttgtca
    17461 actgtagtgg gttgaagtca gctaagctcg agaaaggaca aatttcgtcc tttctttttt
    17521 gatgttcaaa gcgataaaaa tccgtttttt gaagttttca aagtttcgaa aaccaaaagc
    17581 attgcgcttg ataagtttga tgagattatt ggtcgcttcc agtttggcat tagaatagtg
    17641 tagttgaagg gcgttgataa tcttttcttt atctttgagg aaggttttaa agacagtctg
    17701 aaaaatagga tgaacctgct taagattgtc ctcgataagt tcgaaaaatt tctctggttc
    17761 cttattctgg aagtgaaaaa gcaagagctg atagagctga tagtggtgtt tcaagtcttc
    17821 cgaataactc aaaagcttgt ttaaaatctc tttattggtt aagtgcatac gaaaagtagg
    17881 acgataaaat tgcttatcac tcagtttacg gctatcctgt tgtatgagct tccagtagtg
    17941 cttgatagcc ttgtgttcat gggattttcg atgaaactga ttcatgattt ggacacgcac
    18001 acgactcata gcacggctaa gatgttgtac aatgtgaaag cgatcaagaa cgattttagc
    18061 attcgggagt gaaacagtct aggagactgt ttcagcctga gcctagaaat ttgaaagcga
    18121 agctgtttag ccaagtcata gtaagggcta aacatatcca tagtaat
//
LOCUS       DAVLTM010000032        18142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLTM010000032 DAVLTM010000000
VERSION     DAVLTM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.032"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            92..322
                     /locus_tag="VYN16_000940"
     CDS             92..322
                     /locus_tag="VYN16_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1403491.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
     gene            591..2204
                     /locus_tag="VYN16_000941"
     CDS             591..2204
                     /locus_tag="VYN16_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1403492.1"
                     /translation="MEFMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            2334..2960
                     /gene="gmk"
                     /locus_tag="VYN16_000942"
     CDS             2334..2960
                     /gene="gmk"
                     /locus_tag="VYN16_000942"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1403493.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            2985..3299
                     /locus_tag="VYN16_000943"
     CDS             2985..3299
                     /locus_tag="VYN16_000943"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1403494.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            3365..5761
                     /locus_tag="VYN16_000944"
     CDS             3365..5761
                     /locus_tag="VYN16_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1403495.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGKADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            5774..6709
                     /locus_tag="VYN16_000945"
     CDS             5774..6709
                     /locus_tag="VYN16_000945"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1403496.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            6702..8015
                     /gene="rsmB"
                     /locus_tag="VYN16_000946"
     CDS             6702..8015
                     /gene="rsmB"
                     /locus_tag="VYN16_000946"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1403497.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDISSIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            8030..8770
                     /locus_tag="VYN16_000947"
     CDS             8030..8770
                     /locus_tag="VYN16_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1403498.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIITSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            8767..10746
                     /gene="pknB"
                     /locus_tag="VYN16_000948"
     CDS             8767..10746
                     /gene="pknB"
                     /locus_tag="VYN16_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1403499.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            10861..11619
                     /locus_tag="VYN16_000949"
     CDS             10861..11619
                     /locus_tag="VYN16_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1403500.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            11621..12070
                     /locus_tag="VYN16_000950"
     CDS             11621..12070
                     /locus_tag="VYN16_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1403501.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            12132..12758
                     /locus_tag="VYN16_000951"
                     /pseudo
     CDS             12132..12758
                     /locus_tag="VYN16_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            12826..13074
                     /locus_tag="VYN16_000952"
     CDS             12826..13074
                     /locus_tag="VYN16_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403502.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            13205..14377
                     /locus_tag="VYN16_000953"
     CDS             13205..14377
                     /locus_tag="VYN16_000953"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1403503.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            14377..15651
                     /locus_tag="VYN16_000954"
     CDS             14377..15651
                     /locus_tag="VYN16_000954"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1403504.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(15686..16651)
                     /locus_tag="VYN16_000955"
     CDS             complement(15686..16651)
                     /locus_tag="VYN16_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1403505.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(16632..18086)
                     /locus_tag="VYN16_000956"
     CDS             complement(16632..18086)
                     /locus_tag="VYN16_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1403506.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMKFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAINNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRVLAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNSQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         5325 a         3609 c         4258 g         4950 t
ORIGIN      
        1 aatagataga aaagaaaatc ttgtacaagt tcctgacaat ttctttcttt tcgtgtataa
       61 tagtggaaaa gagctaaaac gaggtatggt tatggaagca gtcctttact caacattccg
      121 aaatcattta aaggactaca tgaagaaggt aaatgatgaa tttgagcctt tgacggtggt
      181 caataaaaat ccagatgagg acattgtagt tctttcaaag agcgaatggg acagtattca
      241 ggaaaccctg agaatcgccc aaaataagga gttatcagac aaggttttac gaggaatgga
      301 taaaattcgc gcaggacact agtctaattt acagagaata cctgagtgaa atcttgctgt
      361 tgttagaaac aaacttcttg tatttcataa tcattaggaa gtctactgca aagtaggctt
      421 tttctcaagt ctctactaaa ctactatttc taaaaaacaa gtttattttg gtaaatgtgt
      481 tccaaattcc ccaaaacata ttctatttta ggtttgtgaa aatcactttt ttatggtaga
      541 atgtaaaaga atgtatgtca ttcggaataa caataaaatg aggtaaaaac atggagttca
      601 tgtcgcttgc gattgctgtt tttgccgtca tcattggttt agtcattgga tatgtcagca
      661 tctcagctaa gatgaaatca tctcaggaag ctgcagagtt gatgctttta aatgctgaac
      721 aagaagcaac taatttacgt ggacaagctg agcgtgaagc ggatttactt gttaatgaag
      781 ccaaacgtga aagcaagtct cttaaaaaag aagcactatt ggaggccaaa gaagaagcca
      841 gaaaataccg tgaagaagtg gacgctgaat tcaaatcaga acgtcaagaa ctcaaacaaa
      901 tcgaaagtcg tttgacagag agagctacta gccttgaccg taaggacgac aatttgacga
      961 gtaaagaaca aacacttgaa caaaaagaac aaagtatttc tgatagagcg aaaaaccttg
     1021 atgcgcgtga agagcaatta gaggaagtcg aaagacaaaa agaagcagaa ctagagcgta
     1081 ttggtgctct gtctcaggca gaagcacgag atattatctt ggctcagaca gaggaaaact
     1141 tgaccaggga gattgccagt cgcattcgcg aagctgagca agaggtcaag gaacgttctg
     1201 acaaaatggc caaggacatc ttggttcaag ctatgcaacg tatcgctggt gaatatgtag
     1261 cggagtcaac aaactcaaca gttcatctgc cagacgatac tatgaaggga cgcattattg
     1321 gtcgtgaagg tcgtaacatt cgtacctttg aaagtttgac aggggtcgat gtgattatcg
     1381 acgatacacc agaagtggtg accttgtcag gatttgatcc gattcgtcgt gagattgccc
     1441 gtatgactat ggaaatgttg ctcaaagatg gtcgtataca tccagctcgt atcgaagagt
     1501 tggttgagaa aaaccgtcaa gagattgaca ataagattcg tgaatacggt gaggctgctg
     1561 cctatgaaat tggtgcgcca aaccttcatc cagacttgat gaagattatg ggacgtttgc
     1621 agttccgtac ttcatatgga caaaatgttt tgcgccattc gattgaggtt gctaagttgg
     1681 ctggtatcat ggcgagcgaa cttggtgaaa atgcggctct tgcccgtcgt gctggattcc
     1741 ttcacgatat cgggaaagcc attgaccatg aggttgaagg tagccacgtt gaaatcggta
     1801 tggaattggc ccgtaagtac aaggaacccc cagttgtggt gaatacgatt gctagtcacc
     1861 acggagatgt tgaagctgag agcgtgatag cagttatcgt cgctgcagca gatgccttga
     1921 gcgcagcccg tccaggtgct cgtagtgagt ctcttgaaag ctacatcaag cgtctccatg
     1981 atttggaaga aattgctaac ggctttgaag gagtgcaaac tagctttgcc cttcaagcag
     2041 gacgtgaaat tcgtatcatg gtcaatccag gaaaaatcaa ggacgacaaa gtcacaatct
     2101 tggctcacaa agttcgtaag aaaattgaaa acaatctcga ttatccagga aatatcaagg
     2161 taaccgtgat tcgcgagctt cgtgcagtag attatgctaa ataaataaga aagagcagtt
     2221 gaaaaattac tgcttttttg ttacactaga tagaaagact gtaaaggata atctggcttt
     2281 ttgccattat tctagagaaa tgttatttca cagattgact aaaaggagac acaatggcag
     2341 accgaggctt actaatcgtt ttttctggtc cttcaggggt tggaaaagga acggttagaa
     2401 gagagatttt tgagagttct gaaaaccaat ttcaatattc tgtatcgatg acgacacgcg
     2461 cacaacgtcc tggagaagtg gacggtgttg actatttctt ccgtactcgt gaagaatttg
     2521 aagagctgat tcgtcaagga cagatgttgg aatacgcaga atatgtcggt aactactatg
     2581 gaactcctct gacctatgtc aatgaaacct tggacaaggg aatcgatgtt ttccttgaaa
     2641 ttgaagttca gggtgctctt caggtcaaga aaaaggttcc agatgctgtc tttatctttc
     2701 tgacaccacc agatttggat gaattgcaag atcgcttggt aggtcgtgga acagatagtg
     2761 cagaagtgat tgcccaacga atcgaaaagg ccaaggaaga aattgccctc atgcgtgagt
     2821 atgattatgc gattgtcaac gatcaggtgc ccctagctgc tgaacgtgtc aaatgtgtga
     2881 ttgaagcaga acacttctgt gtggatcgtg tcattggtca ctatcaggag atgttaccaa
     2941 aatctccaac tacccgataa aatttagaaa ataggtacaa gcaaatgatg ttaaaaccct
     3001 ctattgatac cttgctcgac aaggttcctt caaaatattc actcgtaatc ttggaagcaa
     3061 aacgtgccca cgaattggaa gcaggtgccc cagcaactca aggtttcaag tctgaaaaat
     3121 caactcttcg cgctttagaa gaaatcgaat caggaaacgt tacaattcac ccagatccag
     3181 aaggaaaacg tgaagcagtg cgtcgccgta tcgaagaaga gaaacgccgc aaagaagaag
     3241 aagaaaagaa aatcaaagag caaattgcta aagaaaaaga agatggtgaa aaaatttaag
     3301 gttgggggga ctcaatctta ttttttctat tgcaagaatg tactgacaag gaggaggtga
     3361 gaagatggcc ctagctaaga ttatcgtaga tgtgcccttg atgcagacgg accagcccta
     3421 tagttacagg attccggagg aatttgaggg aatgctggaa gttgggatgc gggttcatgt
     3481 accttttggt aagggtaatc gcctgattca agggattgtt cttggtttgg agtcccaatc
     3541 agatggagaa gagatggagc aagatttaaa agatattgca gaggtgctgg atttttctcc
     3601 tgttctcacg ccagaacaac tctggctggc tgaggagtta cgcaaatctg tcttctccta
     3661 caaaatctct atcctcaagg ccatgcttcc aggatttctg aattctagct atgacaagat
     3721 tctctatcct ctggaaggct tgagtcagga agaacgagta cgcctgtttg gttcagaaga
     3781 ttcgctagcc ttttcttcac tagaccttgc caagcaagct gaaatgatgc gtttgactag
     3841 aaaaggcctg cttggtctgg aatatcaggc agtcgatcaa aagaaggtca agacccagtc
     3901 ttggtatgag gttgaccatg cccaattaga aggtgttgag atttctacac gtgccaagaa
     3961 aaagttggaa ctgagagatt atctgctgtc tcatccagag agcgcttcct tggctagctt
     4021 gttagagtcc tactcgcgag agcaagtcaa cttctttgtg gatcaaggtg ctgttaccat
     4081 agtccaaaag gaagttcaac gctcggctgc ttattttgaa ggaattgaag caagtagacc
     4141 tttggagtta aatccagaac aaagacaggc gcgtgatgcg gttgtgagtt ctattggcag
     4201 ttctcaacct ccctttctac ttcaagggat tacaggaagt gggaagaccg aggtttactt
     4261 gcagattatc caaggtgccc tggataaggg caagacagct attttgctgg tacctgagat
     4321 ttccttgact cctcagatga ccgagcgttt tatagcacgt tttggggaca aggtagccat
     4381 tcttcactca ggattatcca atggtgaaaa gtatgatgaa tggcgcaagg tggagcgtgg
     4441 cgatgcccaa gttgttgttg gtgccagatc tgctatcttt gctcctctga aaaatctggg
     4501 tgtcatgatt attgatgaag agcatgaagc ggcttataag caggacagca atccccgtta
     4561 ccatgccaga gaggtagcta ttttacgggc tcagtataat caagcaactc tggtacttgg
     4621 atctgcaacg ccgagcttag agagccgggc gcgtgctggc aaaggcgtct atcaacactt
     4681 acgtctaacc caacgtgcca atcctttggc tacaatccct gaggttcaag tgattgactt
     4741 tcgagactat atcggacaaa atgagacgtc aaactttacg cctcctttgc tagaggctat
     4801 ccaagaccgt ctggttaaaa aagagcaggt ggttctcatg ctcaatcgcc gtggttattc
     4861 tagctttgtc atgtgtcggg agtgtgggac ggtggatact tgtcccaact gcgatatttc
     4921 tctgaccttg catatggata ccaagactat gaactgccat tattgtggtt tctcgaagga
     4981 tattcctcag gtctgtccta actgtaagag ccacagtatt cgttactatg ggacgggaac
     5041 tcagaaggct tatgatgagc tagcagaact ctttccccaa gctcgcattc tgaggatgga
     5101 tgtggatacg actcgaaaaa aaggcagtca ccaagccttg cttgatcagt ttgggcgagg
     5161 gaaagcggat attttacttg gtactcagat gattgcaaag ggtttggatt ttcccaatgt
     5221 taccctagtc ggagttctaa atgcggatac ggccttgaat ctgcctgatt tccgttcttc
     5281 tgagagaacc ttccagctct tgactcaggt ggcaggtcga gcaggtcggg ctgaaaaagc
     5341 aggtcaagtc ttgatacagt cctacaattc gcagcactat gctattcgat ttgccaagga
     5401 tcaagattac gaaggctttt atgcctatga aatgggaatc agacgacaac tcggctatcc
     5461 accttactat ttcacgattg gtattaccct ttctcacaag aaagaagaag aggttttcaa
     5521 acgtgcctat gaagtcatga acattttgcg gtcaggcttg tcagagacca gtcccattct
     5581 ggggccaacg ccaaaaccga ttgcccgtac ccacaacctc tatcattacc agattttaat
     5641 taaataccgt ttagaagatg agctggggcc gaccctcaac caggtcttgg ccttgactca
     5701 agaacgggaa aatagtgagc tccgtctcag cattgaccat gagccgcagc aatttttata
     5761 aaaaggagaa gatatgacaa aactaatctt tatggggacc cccgactttt cagcaacagt
     5821 cttaaaagga cttttgacag atgaccgtta cgaaattcta gccgttgtga cccagccaga
     5881 ccgtgctgtt ggtcgtaaaa aagttatcca agaaacccca gtcaagcagg ctgccaagga
     5941 agcaggacta tctatctacc aacctgaaaa attatctgga agtccagaga tggaagatct
     6001 tatgaagcta ggagcagatg gaattgtgac tgctgctttt gggcagtttc tcccaagcaa
     6061 actccttgat agcatggact ttgctgtcaa cgttcatgcc tccctccttc ctagacaccg
     6121 tggtggtgcg cctatccatt atgccttgat tcaaggggat gaggaagctg gtgtgaccat
     6181 catggaaatg gttaaggaaa tggatgcagg agatatgatt tctcgtcgca gcattccgat
     6241 cacagatgag gacaatgttg gcaccttgtt tgaaaaattg gcgctagttg gtcgtgattt
     6301 gcttttggac actctgcctg cctatattgc tggtgatatc aaacctgaac cgcaggatac
     6361 gagtcaggtt accttctctc caaatataaa gccagaggaa gaaaaactgg actggaacaa
     6421 aaccaatcgt caactcttta accaaattcg tggaatgaac ccctggcctg ttgcccatac
     6481 tttccttaag ggcgaccgct ttaagattta tgaagcccta ccagtagaag gtcagggaaa
     6541 tccaggtgag attctctcta tcggcaagaa agaattgatt gtcgcaacgg ctgaaggggc
     6601 tctatccctc aaacaagtgc agccagctgg taagcctaag atggacattg cttccttcct
     6661 caacggagtt ggacgtacat tgactgtagg agaacgattt ggtgactaaa gtagaaacgg
     6721 ctagaagttt agctctagca gtgctagagg atgtttttgt gaaccaagca tactcaaata
     6781 tcgccttaaa taagcatcta aagggaagtc aacttttggc agcagacaag ggcttagtga
     6841 ccgagctggt ctatggaacg gtagcccgta aactgactct ggaatggtac ctatcccact
     6901 ttatcgaaga cagagaccag ttagacagct ggctctatgt ccttcttctc atgagtgcct
     6961 accaactccg ctatttggac aagattccag atcatgctgt ggtcaatgaa gcagtggaat
     7021 tggccaaact tcgtaaaaaa ggcagtgaaa aattggtcaa tgccctcctt cgccgtatct
     7081 tgcgtgaagg ctggccagat atttctagca tcaagcgaaa aaacaagcgt gactccattg
     7141 cctattctct cccagtttgg ctagttgcca aactcaagga agaatacgga gaggagagag
     7201 caaaagccat ctttgaaagc ttgttagtgc gaaacaaagc cagtattcgt gtaacagacc
     7261 taagtcgaaa agaggaaatc caagccttgt tggaggcgaa taattccctt ttagcaacca
     7321 ctggtctggt taaggagcag gggcattttg cagggcataa tttgtttgcg gatggagcca
     7381 ttaccatcca agacgagtcc agtcagctgg ttgctccgac gcttgattta caaggtgatg
     7441 agcgagtgct tgatgcctgt gcggctccag gtgggaaaac agcccatata gcctcttatc
     7501 ttacgacagg tcaggttact gctctggact tgtacgacca caagttggat ttaattcaag
     7561 aaaatgccca acgtctggga gttgcagatc gggttcaaac tcaaaaattg gatgccagaa
     7621 aggtgcatga gttttttgac caggattcct ttgataagat tttggtggat gctccttgtt
     7681 caggaatcgg tcttttgcgc cgaaaaccag atatcaaata caataaagaa acggcagatt
     7741 tcgcgtcctt gcaggaaatt cagttagaaa tattaggtag tgtttgtcaa atactaggca
     7801 aaggtggtat aataacttat agcacctgca ctatcgtctc agaggagaat tttcaagtcg
     7861 ttaaggcctt tttagagagt catcccgagt tcgagcaggt aaaactagaa catgaatgta
     7921 aggatatcat gaaagacggc tgtatcctca ttacacctga attgtatgga agtgatggat
     7981 tcttcatcag tcaatttcgc aagatatcgg attaggaagg aactgacaca tggaaatttc
     8041 attattaaca gatgttggtc agaaacgaac aaataaccaa gactatgtca accactatgt
     8101 caatagagct ggacgtacca tgattatttt agctgatggg atgggaggtc atcgcgcagg
     8161 gaatatcgct agtgaaatgg cggtcacaga cctgggtgta gcttgggttg atactcagat
     8221 cgatacagtc aatgaagtgc gtgaatggtt cgcccattac ctagaaattg aaaatcaaaa
     8281 gattcaccag cttggtcagg atgaagctta cagaggcatg ggaactactt tggaagtcct
     8341 tgctattatt gataatcagg ctatctatgc tcatattggt gattcgcgta tcggcttgat
     8401 tcgtggagaa gaataccatc agttgacgag tgatcattct ttggttaatg aattgctcaa
     8461 ggctggtcaa ttgacaccag aagaggcaga agctcatccg caaaaaaata ttatcaccca
     8521 gtctattggg caaaaagatg aaattcagcc tgattttggg acagttatcc ttgagtcagg
     8581 tgactatctc ttgctcaata gtgacggctt gaccaacatg atttcaggca gtgagattcg
     8641 tgatattata accagtgata ttcctttagc agataaaacg gagacacttg ttcgttttgc
     8701 taacaatgca ggaggtttag acaacattac ggttgccctt gtttctatga acgaggagga
     8761 tgaagaatga tccaaatcgg caagattttt gccggacgct atcggattgt caaacagatt
     8821 ggtcgaggag gcatggcgga tgtctaccta gccaaagact taatcttaga tggggaagaa
     8881 gtggcagtga aggttctgag gaccaactac cagacggacc cgatagctgt agctcgtttt
     8941 cagcgtgaag cgagagctat ggcagatcta gaccatcctc atatcgttcg gataacagat
     9001 attggtgagg aagacggtca acagtatctt gcaatggagt atgttgctgg actagacctc
     9061 aaacgctata tcaaggaaca ttatcctctt tctaatgaag aagcagtccg tatcatggga
     9121 caaattctct tggctatgcg cttggcccat actcgaggaa ttgttcacag ggacttgaaa
     9181 cctcaaaata tccttttgac accagatggg acggccaagg tcacagactt tgggattgct
     9241 gtagcctttg cagagacaag tctgacccag actaactcga tgttgggctc agttcattac
     9301 ttgtcaccag agcaggcgcg tggttcgaag gcgactgtgc agagtgatat ctatgccatg
     9361 gggattattt tctatgagat gttgacaggc catatccctt atgacgggga tagcgcggtg
     9421 accattgccc tccagcattt ccagaaaccc ctgccgtccg ttattgcaga aaatccatct
     9481 gtacctcagg ctttagaaaa tgttattatc aaggcaactg ctaaaaagtt gaccaatcgc
     9541 taccgctcgg tttcagagat gtatgtggac ttgtctagta gcttgtccta caatcgtaga
     9601 aatgaaagta agttaatctt tgatgaaacg agcaaggcag ataccaagac cttgccgaag
     9661 gtttctcaga gtaccttgac atctattcct aaggttcaag cgcagacaga acacaaatca
     9721 atcaaaaacc caagccaggc tgtgacagag gaaacttacc aaccacaagc accgaaaaaa
     9781 catagattta agatgcgtta cctgattttg ttggccagcc ttgtattggt ggcagcttct
     9841 cttatttgga tactatccag aactcctgca accattgcca ttccagatgt ggcaggtcag
     9901 acagttgcag aggccaaggc aacgctcaaa aaagccaatt ttgagattgg tgaggagaag
     9961 acagaggcta gtgaaaaggt ggaagaaggg cggattatcc gtacagatcc tggcgctgga
    10021 actggtcgaa aagaaggaac gaaaatcaat ttggttgtct catcaggcaa acaatccttc
    10081 caaattagta attatgtcgg ccggaaatct tctgatgtta tcgcggaatt aaaagagaaa
    10141 aaagttccag ataatttgat taaaattgag gaagaagagt cgaatgagag tgaggctgga
    10201 acggtcctga agcaaagtct accagaaggt acgacctatg acttgagcaa ggcaactcaa
    10261 attgttttga cagtagctaa aaaagctacg acgattcaat tagggaacta tattggacgg
    10321 aactctacag aagtaatctc agaactcaag cagaagaagg ttcctgagaa tttgattaag
    10381 atagaggaag aagagtccag cgaaagcgaa ccaggaacga ttatgaaaca aagtccaggt
    10441 gccggaacga cttatgatgt gagtaaacct actcaaattg tcttgacagt agctaaaaaa
    10501 gttacaagtg ttgccatgcc gagttacatt ggttccagct tggagtttac taagaacaat
    10561 ttgattcaaa ttgttgggat taaggaagct aatatagaag ttgtagaagt gacgacagcg
    10621 cctgcaggta gtgtagaagg catggttgtt gaacaaagtc ctagagcagg tgaaaaggta
    10681 gacctaaata agactagagt caagatttca atctacaaac ctaaaacaac ttcagctact
    10741 ccttaaaagc agatggataa tcaaaatgag taatcaatct ttgtcatgat ttcatggcaa
    10801 agattttttt tgagtccaga tttgtgatag aatagaggga gttgataaaa ggaggaaagc
    10861 atggaagaat caaaagaatt aaatgccgtc attgatgtga ttatgctagc ggggactatt
    10921 ctccttaaaa gtggctcaga aatccatcgt gtagaagata ccatgattcg aatcgcgcat
    10981 tcgcagggga ttgtggattg caatgtcctt gccatgcctg ccgctatctt tttctctatt
    11041 gaaaatacca atatttcgcg catgaagcgc gtgacctcct cttcttataa catcgaaaaa
    11101 gtctgcgatg tgaaccagat ttctcgtcag ctagttgggg ggcagattga cttagaaaca
    11161 gcctttaagc aattgacggc cttgcaagct caaccccttc cctatactaa gttgcaggta
    11221 actctggctg cgacctttag tgctcctttc ttttcagtta tgtttagcgg aaatatctac
    11281 gacgcacttg gggcaggagt ggcgacctta tttggttttg ccttttccct ctatgtggaa
    11341 aaatttatcc gaattccctt tgtgacagcc tttgctggag cctttgtctt tgggataatt
    11401 gcccagtttt gggctcgcta cacaggtttt ccttcaacgg cagatttgat tatagctggt
    11461 gcggtcatgc cgtttgtacc aggtattgcc ttgaccaatg cggtccgtga tattatgacc
    11521 aaccacataa actctggtat gagtaagatg tttgaatccc tgctcattac cctcgcttta
    11581 ggggcaggaa cttctgtcgc cttggtattg atgaactaat atgacactaa caaccttttt
    11641 attacaagca gtagcaagtt ttcttgccat tatcactttt ttaatcgtac tcaatgtgca
    11701 acggtctatg ctcttacctg gagggatttt gggcatgact gtctggctaa tctatctctt
    11761 gctcaaggaa ccgaccaatg tcattgtagc taccttcatt gcagccatta ttggttcttg
    11821 tgtcagccag attttaagta ttctttataa gacacctgct gtggtcttta tcttggccat
    11881 tttggcaccg ctggttccag gttatctctc ctaccgaaca actgcctttt ttgtgacagg
    11941 ggactataat aaagcactgg caagtgcgac cttggttgtc atgttggctt tggtaatctc
    12001 tattggaatg gctagcggaa cagtgattct cagactgtat cattatataa aaacacatcg
    12061 agtatcgtag actttacaga aataaaagaa ttttctgaaa aatgagataa ataaattaac
    12121 aacgctttct atatgtgcga gaataccgca cttatgaaga aattgcggct gattttggta
    12181 tccacgaaag caacttactc cgtcggagcc aatgggttga agtaactctt gttcaaaggt
    12241 ggtgttacga tttcaagaac tcctctcagt tctgaagaca cggtaatgat tgatgcgacg
    12301 gaagtaaaaa tcaatcgccc taaaaaaaac aattagcgaa tgattctggt aaaaagaaat
    12361 ttcacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt tctttggata
    12421 tcactgtgaa ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac
    12481 aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata tatcctcaag
    12541 cacaaactcc acgtaaatcc agcaaactca agccgctaac agttgaagat aaagcctata
    12601 accatgcgct atctaaggag agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt
    12661 ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt
    12721 tgattgctgg cattatcaat catgaactag gattctagtt ttgcaggaag tctactactt
    12781 tccttattgt ctgtaaatct actgaccttg ttgtttatcc cagtcatggt ttctagttcg
    12841 ggctcagagt ttcaaagtgg atggcaagag catcaattga ttgctgagaa ggttagtaaa
    12901 acacttgata agacatttga taaggatgtc agagaaattc cgaccagtca gttttatcaa
    12961 aaatttgtag atgagatggg aaggacttac tcaggaaatt tgatcctcca ggagctgata
    13021 actgtgaatg gagcttataa agctacttat atcggtgaac tctctagcaa ctaatcattc
    13081 tttaaaatga tgaccaataa gaggcttgat ttggtgaatc aagtcttttt tctctctttt
    13141 actgccattt gtgataaaat agtatagaac gattttttac atgaatgata aaacagaggt
    13201 aaatatgaca atcggtattg ataagattgg ttttgcgacc agtcaatatg tcttgaaatt
    13261 acaagactta gcagaagcga ggggaattga ccctgaaaaa ttaagtaaag gactcttact
    13321 caaggaattg agtattgcgc ccctaactga ggacattgtg accttggcgg ccagtgctag
    13381 tgactctatt ttaactgagc aagaaagaca agaagttgac atggtcattg tggctaccga
    13441 gtcaggaatt gaccagagta aggctgcggc cgtctttgtg catggcttgc tgggcatcca
    13501 gccctttgct cgtagtttcg agattaaaga agcctgttat ggagcgactg ctgcccttca
    13561 ttatgccaaa ttgcatgtgg aaaattctcc ggagtccaag gtcttggtca ttgccagtga
    13621 tattgccaaa tacggtattg aaactccagg agaaccaact caaggtgctg gaagtgtagc
    13681 tatgttgatt acacaaaatc cacgcatgat ggcctttaat aatgacaatg tagctcagac
    13741 ccgtgacatc atggatttct ggcgaccaaa ttactcgaca actccttatg taaatggtgt
    13801 ttattctacc caacaatact tggatagttt gaaaacgact tggcttgaat atcaaaaacg
    13861 ctaccagctt actctggatg attttgcggc tgtttgtttc cacttgcctt atcctaaatt
    13921 agcgctaaaa ggcttgaaaa aaatcatgga taagagcctg cctcaagaga aaaaagacct
    13981 cttacaaaag cattttgacc agtctattct ctacagtcaa aaggtgggga atatctacac
    14041 aggttcactt ttccttggac ttttgtctct cttggaaaat acagatagct tgaaagctgg
    14101 ggataaaatc gccctttata gttacggaag tggagctgtg gctgagttct tcagtggtga
    14161 attggttgaa ggatatgaag cttatttgga taaagaccgt ttgaacaagc tcaaccaacg
    14221 aactgcccta tccgttgcag actatgaaaa ggtctttttt gaggaagtaa acttggatga
    14281 aacaaactct gcccagtttg ctggctatga aaatcaagat tttgccttgg ttgaaattct
    14341 tgaccaccaa cgccgttata gcaaggttga aaaataatga agataagttg gaatggattt
    14401 tctaaaaaat cataccaaga gcgcctcgag ctgctaaaag ctcaggcgct ccttagtcct
    14461 gagagacaag ctagtctgga gaaggatgaa cagatgagtg tgactgtggc agaccagctg
    14521 agtgagaatg tggtgggaac tttttctctg ccttattcgc tggttccgga ggtacttgtc
    14581 aacggtcagg aatacaccgt tccctatgtg acagaagaac cctctgtggt tgcggcggcc
    14641 agctatgcca gcaaaatcat caagcgtgca ggtggtttta ctgcacaagt ccatcagcgc
    14701 cagatgattg ggcaggtagc cctttatcaa gttgctaatc ctaaactagc gcaagagaag
    14761 attgccagca agaaagcgga gctcttggag cttgccaatc aagcctatcc ttctatcgtt
    14821 aaacgtgggg gtggggcgag tgacctgcat gtcgagcaga taaaaggtga accagacttt
    14881 ctcgttgttt atattcatgt tgatacccag gaagccatgg gtgccaatat gctcaacacc
    14941 atgctggaag ccttgaaacc agtcttagaa gaactcagtc agggacagag tctcatggga
    15001 atcctgtcca actacgcgac cgattctctg gtgactgcaa gctgtcgcat ggcctttcgc
    15061 tacttgagcc gccaaaagga tcaaggacga gagattgcgg agaaaattgc attggctagt
    15121 cagtttgcgc aggctgatcc ttaccgagct gctactcata ataaaggaat ttttaatggt
    15181 attgatgcga ttttgattgc cactggtaat gactggcgtg ccatcgaagc tggggcccat
    15241 gcctttgcca gtcgagatgg acgctatcaa ggtcttagct gctggacgct ggaccttgaa
    15301 agagaagaat tggtcggtga gatgaccctg cccatgcctg tagcgactaa gggtggctct
    15361 atcggcctca acccacgtgt agctctcagt catgatctac taggaaatcc ttctgccaga
    15421 gaattagccc agattatcgt gtccatcggt cttgctcaaa attttgcagc cctcaaagcc
    15481 ttggtaagta cgggcatcca gcaaggccac atgaaactac aggccaaatc cctagctctc
    15541 ctagctgggg ctagtgaatc tgaagttgct cccctagtag agcgcctcat ctcagataaa
    15601 acctttaacc tagagacagc ccagcgctat ctcgaaaatt taagatcata aaaactcaga
    15661 cgaatcggtc tgagttttct tgtgtttaaa tactttttcc tggtaatagc gtaactggta
    15721 agaagtaacc agttgtggcg acttccttgc cttcaatctt ttgcaagaga agatcaatag
    15781 tgagacaagc aatctcttcc aaaggttgct tgatagtagc caattgaggg tagtaatttt
    15841 cgataaagta ggtcccatca tagccgatga ccttgagctc ttttgggaca gaaatgccca
    15901 attcttgagc gattttaatg accagaatag ctgtcaaatc atccgaagca aaaatggcat
    15961 ctggtttttc ccgggtcaag atattcttga tttccatttc ttttctgacg ggagaaaagt
    16021 cactggaaac attgataata ggagcttttg ggagtacgga tgcaaaacca gcgtggcgca
    16081 gtccggttgg cgaattggaa ttgtcattcc ctgtaatcat gatgatagac tgggcacctg
    16141 tcttgaccaa ggtttgggca gcaagaaccc caccagcata gttgtcagag gagacgacag
    16201 ggatgtctgg cgataggttt cggtcaaagg aaataatcgg cgctgtcaca cgattgtagt
    16261 cttcgattcc taggttgtga ctaccagaaa tgatgccgtc cacctgattg gcttccaaca
    16321 tttcgatgta ttcgcgttcc ttctcagaat catgttcact gttgcagatg atggtcttgt
    16381 aaccattttt gaagagttgg tgttccaatt tatcaatcaa ttctgcatag aaaacattgg
    16441 aaatattggg gaaaatcaag ccgattaact tagctgattt tccttgcaga ctacgagcca
    16501 ggttgttggg tttatagccc aattctcgca tggcttcatt gactttttgg atggttttct
    16561 cagatagata ccctttttta ttgataaccc gagaaacggt agtaggactg acgcctgcaa
    16621 gtttggcgac atcagttagt tttgcgacca taatctaatt catagtaagt tccagttggg
    16681 tttccagatt tgatcaggat accattttgg tccgcatgtg ggaagacacg accagaaaat
    16741 actttttctc ctttattgat gaaaatttca aagacagagt tatcgatgaa gattgtagca
    16801 gtagtagcct gattctcgat agggcaagaa cgagttgtcc caaattcttg ggcatactgt
    16861 tctccagcct ggctacgatc cactgtcact tgactgttta caagatcaaa gttgattgaa
    16921 agtcccttac cttctttatc agcaagtaag acaatctcgc tctggctatt agcttccaag
    16981 ttgagttcaa gttcgtaagt gttcttggtt tgggaacggt ttgagaaggc ttcttcagaa
    17041 gcacgaaggt ccttaatagc agcgactgga tactggtaga gcttgtcgtc tttgatagtg
    17101 agttccttga ccaaagagaa ggttccttgg tggtcaaaac ggtcagatgg gtaagaaaca
    17161 tctggcaaac caagccagct aactgctaga acacgcccat caggagcgtt gaaggcttga
    17221 gttgcatagg cttcgaaacc gtaatccatg ttttgaagtt gagacacatc taccattttg
    17281 gcatttttag ggtcaaagga agccccaatc ttatacatat ttggaaagat attatcgtag
    17341 tctagaactt tcttatccaa tccttgtgga cagtagagaa ggacaggctg ttcctctaca
    17401 aagaccagat taggacattc catcatgtag gcagtacggt cgttagcaaa gtcaaggtcg
    17461 ccaactgctt gccagtttgt gtagtcgtta ttgatagcct tgtagagacg gacgaagcct
    17521 tttttctcca agtcctgtcc accgacgaca gcatagtatt gacctttaaa gttaaaaatt
    17581 tgtggatcgc ggaagtggtc agtagagtct gctggctggt caatcaagat cttgtcaatc
    17641 tttgtaatct taccctcctt gtccatcaaa gcaccgatct ggtatgggtg acggatccag
    17701 ttttcatcgc gaacatttcc tgtataaaat aggaataagt tatcgccaaa tttcatggca
    17761 gaaccagagt aggcaccgtg gctatctaat ggagtatctg gtaaaacttt gattccagtt
    17821 tctttaaagt gaaccaaatc atcactttct agctgtgccc aagatttcaa accgtgggct
    17881 gcaccaaaag gaaaattctg gtaaaagagg atccacttgc catcaaagta agaaaagcca
    17941 tttgggtcgt tgagaagtcc tgtttttggc tcaacatggt aatgagtatg ccatggagat
    18001 tgtgccatat tttcctttat atgttgaatt tcttcttgcg tccaatcttg ataaagtctg
    18061 taacgacgct cagttgtcca ttccattcat tcctccaaaa atcttatcac ttttaatagt
    18121 aactgttttc gataaaaaat gc
//
LOCUS       DAVLTM010000033        17428 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLTM010000033 DAVLTM010000000
VERSION     DAVLTM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.033"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            351..1514
                     /locus_tag="VYN16_000957"
     CDS             351..1514
                     /locus_tag="VYN16_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1403507.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1511..2188
                     /locus_tag="VYN16_000958"
     CDS             1511..2188
                     /locus_tag="VYN16_000958"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1403508.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2178..3032
                     /locus_tag="VYN16_000959"
     CDS             2178..3032
                     /locus_tag="VYN16_000959"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1403509.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3051..4118
                     /locus_tag="VYN16_000960"
     CDS             3051..4118
                     /locus_tag="VYN16_000960"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1403510.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4128..5294
                     /gene="aroC"
                     /locus_tag="VYN16_000961"
     CDS             4128..5294
                     /gene="aroC"
                     /locus_tag="VYN16_000961"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1403511.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5304..6407
                     /locus_tag="VYN16_000962"
     CDS             5304..6407
                     /locus_tag="VYN16_000962"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1403512.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6418..6756
                     /locus_tag="VYN16_000963"
     CDS             6418..6756
                     /locus_tag="VYN16_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1403513.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6848..8131
                     /gene="aroA"
                     /locus_tag="VYN16_000964"
     CDS             6848..8131
                     /gene="aroA"
                     /locus_tag="VYN16_000964"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1403514.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGASVIIEKECTRNHTEDMLKQFGGHLSVDSKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8124..8600
                     /locus_tag="VYN16_000965"
     CDS             8124..8600
                     /locus_tag="VYN16_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1403515.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8597..9445
                     /gene="pheA"
                     /locus_tag="VYN16_000966"
     CDS             8597..9445
                     /gene="pheA"
                     /locus_tag="VYN16_000966"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1403516.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAENPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9442..10716
                     /gene="psr"
                     /locus_tag="VYN16_000967"
     CDS             9442..10716
                     /gene="psr"
                     /locus_tag="VYN16_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1403517.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10995..11840
                     /locus_tag="VYN16_000968"
     CDS             10995..11840
                     /locus_tag="VYN16_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1403518.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11830..12933
                     /locus_tag="VYN16_000969"
     CDS             11830..12933
                     /locus_tag="VYN16_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1403519.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12935..13921
                     /locus_tag="VYN16_000970"
     CDS             12935..13921
                     /locus_tag="VYN16_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1403520.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13937..15370
                     /locus_tag="VYN16_000971"
     CDS             13937..15370
                     /locus_tag="VYN16_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1403521.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15399..16433
                     /locus_tag="VYN16_000972"
     CDS             15399..16433
                     /locus_tag="VYN16_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1403522.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16633..17370
                     /gene="mecA"
                     /locus_tag="VYN16_000973"
     CDS             16633..17370
                     /gene="mecA"
                     /locus_tag="VYN16_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1403523.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
BASE COUNT         5190 a         3141 c         3948 g         5149 t
ORIGIN      
        1 aggcttctct ataaaatcaa attttccaga tacctattga agcagattta tttcttcatc
       61 tttcttctac tacctttgtc actaaatttg acagacacat attgctgatt caacctctct
      121 acctataatc tcacagttaa cctgaggcta gtttcatagt ttgttatttg attttcgttg
      181 ataaaaagaa atgctcctcc aagacggaag agcatttttt attgtgattc ttacacattt
      241 acttcctagt tttgcaggaa gtctagtata ccatttttta aaagaagagt aaaaaagcaa
      301 gggtagtttc ttttttgaga attgtctaaa agtttgatat aatggtagtt atgaatagaa
      361 taagagtcag caaaagggtt gaaaagaagc ttgctaaggg gctagtttta ctagaagcca
      421 gtgatcttga gaatgtcaat cttaaggatc aggaagtaga ggtgcagggt caggaaggaa
      481 actttcttgg gactgcctac ctttctcagc aaaacaaggg cttgggctgg tttgtcagca
      541 aagacaaggt ggccttcaat caaactttct ttgaaacgtt gtttagaaaa gccaaagaaa
      601 agagaaacgc ctactatcaa gatgatttga caactgcctt tcgtctcttt aatcaagagg
      661 gagatggctt tgggggtctg acagtggacc tttatggcga ctacgccgtc ttttcttggt
      721 ataactctta tgtttatcag attcgtcaga ctatatcaga agcctttaga caggttttcc
      781 ctgaggtttt aggggcttat gagaaaatcc gctttaaggg ttttgactat gaatctgccc
      841 atgtttatgg tcaagaagca cctgactttt tcactgtttt agaaaatggt gtcctgtatc
      901 aagtctttat gaatgatggc ttgatgacag gaattttcct agaccagcat gaggttcgcg
      961 gtagtttagt tgacggcttg gctatgggta aatccttgct caatatgttt tcctacacag
     1021 cggctttttc agtagctgcg gccatgggag gagctagcca gacaacttct gttgatctag
     1081 ccaaacgttc acgagaattg tctcaagcgc attttcaggc aaatgggctc agcacagacg
     1141 agcatcgttt tatagtcatg gatgtctttg agtatttcaa atatgccaaa cgcaaagact
     1201 tgacctacga tgtgattgtc ctagatccgc ctagctttgc tcggaataaa aaacaaactt
     1261 tctctgtggc caaggattat cacaagttga tttcccagag tcttgagatt ttaaatccgg
     1321 gagggattat cattgccagt accaatgctg ccaatgtttc ccgtcagaaa tttacagaac
     1381 aaattgataa aggttttgca ggaagaagtt accagatttt aaacaaatat ggtcttccag
     1441 cagattttgc ctataataaa aaagatgaaa gtagtaatta cctcaaggtg attagtatga
     1501 aggttagtaa atgaaattaa tcgtttcagt aatgccaaga agtttagagg aggctcaggc
     1561 tctggatgcc acgaggtacc tggatgccga catcattgaa tggcgtgccg actatctgcc
     1621 taaagaagcg attttgcagg tagctccagc catttttgaa aaattcgcag gccgtgagtt
     1681 ggttttcacg ctacgaactc gctccgaagg tggagaaatc gacctttctc cagaagagta
     1741 tatccatcta atcaaggaag ttgcgcaatt ctatcaacca gactatattg attttgagta
     1801 ctatagctac aaggatgttt ttgaggaaat gctggacttc ccaaatcttg ttttgagtta
     1861 ccataatttc caagaaacac ctgagaacat gatggaaatc ttgtcagagt tgacgatcct
     1921 aaatccaaaa cttgttaagg ttgcggtgat ggctcacacg gagcaggatg tcttagactt
     1981 gatgaactat acacgaggct ttaaaaccct caatcctgaa caggaatatg tgaccatttc
     2041 tatgggtaag gtgggcaagg tctctcgtat cactgcggat gtgactggtt cgagttggtc
     2101 ttttgccagt ctggatgagg ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa
     2161 aatcagggag attttggatg aagcttgatg gctatacacg tttagctgca gttgttgcca
     2221 atcctattaa gcattctatt tctcccttca tccacaatag agcctttgag gcgacagcta
     2281 ccaacggtgc ttatgtggct tgggagattg aagcgagtga cttggtagaa acagtggcca
     2341 atattcgtcg ctaccagatg tttggcatca atctgtccat gccctataag gagcaggtga
     2401 ttccttattt ggataagctg agcgatgaag cgcgcttgat tggtgcggtt aatacggttg
     2461 tcaatgagaa tggcaattta attggatata atacagatgg caagggattt tttaagtgct
     2521 tgccttcttt tacaatttca ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta
     2581 aatcaatctt ggcacaggct attttggatg gcgtcagtca gatttcggtc tttgttcgtt
     2641 ccgtttctat ggaaaaaaca agaccttacc tagacaagtt acaggagcag acaggcttta
     2701 aagtggattt gtgtgcttta gaatatgttt ctgaactgca agcaaggatt gccgagtcgg
     2761 atttgctagt taatgccacc agtgtgggca tggatggcca atcctctcct gttcctgaaa
     2821 acatagtctt accagaaact cttttagttg cagatatcat ataccaaccc tttgaaacac
     2881 cgtttttgaa atgggctaga agtcagggca atccagcagt caatggtctg ggaatgttgc
     2941 tctatcaagc tgcagaagct tttcaactgt ggacaggcaa ggaaatgccg acagaagaga
     3001 tttggcagtc tttaacagaa aaataccaat aaagaaaggg agactctcct atgaaaatca
     3061 gaatcgatat tcctcatcat ccttatgata ttcagattga aaaaggttgt atggcccagg
     3121 ctggtcagtg gttgcgagaa ctctggcaac cgcaaaaggt agtcattgtg acagataacc
     3181 atgtagcctc tctctatgca gagaaggtca agctcagcct agaagatgct ggttttcagg
     3241 tagctgtttt tgatttctta gaaggtgaag aaagaaagaa tttaactact gttcagaaag
     3301 tctatgaatt tctagtcaag caaggtctga ctcgtagcga tggaatcgtt gctcttggtg
     3361 gtggcgttgt tggggacctg gctggctttg tagcctctac ctatatgcgg ggtattcact
     3421 ttgttcagat tccgactagt ttgacagctc aggttgattc ttctatcggt ggaaagacag
     3481 gcgttaacac accatttgct aaaaatatgg tggggacctt tgcccaacca gatggggttc
     3541 tgattgatcc acttgttctt gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg
     3601 aagttatcaa gtatggcttg attgaggatc cagaactgtg ggctctcttg acgggactga
     3661 atggttctgt tgagagtatt ttggaacatg cagagacctt gattgaacat tcttgtcagg
     3721 tgaagcgcaa gatggtggtt gaagatgagt tggacaatgg tattcgtctt tacctcaatt
     3781 ttggccacac tattggccat gccatcgaag cgactgccgg ttatggcaag gtcatgcatg
     3841 gagaggctgt tgccatggga atggtacaga tttccaaggt tgctgaggaa aaaggcctca
     3901 tgccagctgg cattacccaa tctatcacag agatgtgtca gaaattcggc ttgcctgttg
     3961 actatgaaaa ttggaaagtt gacaagcttt atcaggctct tactcatgac aagaaagcgc
     4021 gtggtaacac cttgaaattg gtcttggtgc cagagcttgg ttcagcgacc attcacccag
     4081 tttctctgga agagatgaaa gactacttgg taaaataagg agagtgtatg agatatttaa
     4141 ctgcaggaga atcacacggc ccccgtctaa cagctattat tgagggaatt ccagctggac
     4201 ttccattgac agctgaggat atcaatgagg atcttagacg ccgtcagggt ggctacggtc
     4261 gtggtggtcg tatgaagatt gagagtgacc aggttgtctt tacttcgggt gttcgccacg
     4321 ggaagacgac aggggcgcct attactatgg atgtcatcaa taaggaccac cagaaatggc
     4381 tggacatcat gtctgcggag gacattgaag accgccttaa aagcaagcgg aaaattactc
     4441 atcctcgccc aggtcatgcc gatttggttg gggggattaa gtaccgtttt gatgatttgc
     4501 gaaattcttt ggagcgttca tcagctcgtg aaaccaccat gcgggtggca gttggtgcag
     4561 tagccaaacg cctcttggct gagctggata tggagattgc caaccatgtc gtggtctttg
     4621 gtggtaagga aatagatgtt cctgaaaatc tgacagtcgc tgaaattaag caaagagctg
     4681 cccagtctga agtttctatt gtcaaccaag aacgagaaca ggaaatcaag gactatattg
     4741 accaaatcaa acgtgatggt gataccatcg gtggggttgt ggagacagtc gtcggaggag
     4801 ttccagttgg tcttggttcc tatgtccaat gggatagaaa attggatgca agattggctc
     4861 aagctgttgt ctctatcaat gcctttaaag gggtggaatt tggtcttggc tttgaggctg
     4921 gttatcgtaa aggcagccaa gttatggatg aaattctctg gtctaaagaa gacggttata
     4981 ctcgccgtac caataatcta ggtggttttg aaggtggtat gactaatggg caacccatcg
     5041 ttgttcgtgg ggtcatgaaa cccattccta ctctttataa acctcttatg agtgtggata
     5101 tcgaaaccca cgaaccttac aaggcaaccg tggagagaag tgatccgact gctcttccag
     5161 ctgcaggaat ggtcatggaa gcagttgtag caacggttct ggcgcaagaa atcctcgaaa
     5221 aattctcatc agataatctt gaggaactaa aagaagcggt agccaaacac cgagactata
     5281 caaagaacta ttaaggagtt cctatggcaa aaacaatcta tatcgcaggt cttgggttga
     5341 ttggagcctc tatggcactt ggtatcaaac gcgatcatcc agattatgaa attttaggtt
     5401 ataatcgtag tcaagcttcg agagatatcg ccttgaaaga aggtatgatt gaccgtgcaa
     5461 cggatgattt tgctagtttt gctcctttgg cagatgtcat tatcctcagc ttgccaatca
     5521 aacaaactat tgctttcatt aaggagttgg ccaatttgga tttgcgagaa ggcgttatta
     5581 tttcagatgc tggttcgacc aagtcaacca ttgtggatgc ggcggagcag tatttggctg
     5641 gcaagtctgt tcgctttgtc ggggcccatc ccatggctgg tagtcacaag acaggggctg
     5701 cttcggcaga tgtcaatctt tttgaaaatg cctattatat ctttacacct tcaagcctga
     5761 caagtcagga cacgcttaag gaaatgaagg atctgctttc aggtcttcat gctcgtttta
     5821 tcgagattga tgccaaggag catgatcgtg tcacttctca gattagccat tttcctcata
     5881 ttttggcttc tagtctcatg gagcagactg cggtctatgc tcaagagcat gagatggcaa
     5941 ggcgctttgc ggcaggtggt tttcgagata tgacccgaat tgcggaaagc gagccaggaa
     6001 tgtggacctc cattctcttg tccaatagcg agaccattct ggatagaatt gaggatttca
     6061 aggaacgttt ggaagtgatt ggtcaggcca ttagtaaggg agatgaagag caaatttgga
     6121 acttttttaa ccaagcgcgt gaacaacgcc agaccatgga aatccataag cgtggtggtg
     6181 tggatagctc ttacgacctc tatgttgacg ttcctgatga agaagatgtc atcctgcgga
     6241 ttttggaact gctacgtgga acttccttgg ttaacatcca catcaacgag gaaaatcgtg
     6301 aggatattca cggaatccta caaatttcat ttaaaaatgc tcaagacttg gaaagagctg
     6361 agcatctcat aacagaaaat accgactata cagtcgtcat caaataagga gaagaacatg
     6421 tcaaatattt acgatagtgc aaacgaactc agtcgcggtc tacgcggatt accagaatac
     6481 aaggctgtta aagcagctaa agatgcgatt gcagcagatg ctgaggcaag caaaatcttt
     6541 acagaatatc ttgcctttca agaggaaatt caaaaactag cacagacagg tcaaatgcca
     6601 gatgcgtcct tccaagctaa gatggaaggc tttggtaaac agattcaagg aaatagcctc
     6661 ttgtcagaat tctttaccaa gcaacaacaa ttggccattt acctttctga cattgaaaaa
     6721 attgtttttg aaccagtttc agaattgctg aaataagaaa agaacgatcc taaagtcagg
     6781 aatttttaag gaatttctga ctttttatga taaaataggt aaaagtattg caagtatgag
     6841 gtccagtatg aaactaaaaa caaacattcg ccatttacat ggtagtatcc gcgtcccagg
     6901 tgacaagtct atcagccacc gttccattat ctttggaagt ttggctgagg gtgagaccaa
     6961 ggtttatgat attctacgag gtgaagacgt tctttcgacc atgcaggttt ttcgtgacct
     7021 tggtgttgaa attgaggata aagatggggt tattaccatt caaggtgtag gcatggctgg
     7081 cttaaaagcg ccgcaaaatg cccttaatat gggaaattct ggcacctcga ttcgcctgat
     7141 ttcaggtgtc cttgctggtg cagatttcga agtagagatg tttggagatg atagtctttc
     7201 caaacgtcct atggaccgtg tgacccttcc actgaaaaaa atgggcgtca gcatttcagg
     7261 gcaaactgaa cgagacttgc ctccccttcg cttaaaaggg acgaaaaacc taagacctat
     7321 tcattatgag ttgccaattg cctctgccca agtcaagtca gccttgatgt ttgcagcctt
     7381 acaagctaag ggggcgtcag ttattatcga aaaagagtgc acccgtaatc atactgaaga
     7441 tatgttgaaa caatttggtg gtcatttaag tgtggacagt aagaaaatca cagtccaagg
     7501 gccacaaaaa ttgacaggac agaaggtggt cgtaccagga gatatttcca gtgcagcctt
     7561 ttggttagtc gcaggtttga ttgttccaaa ttctcgtcta gtgctgcaga atgtggggat
     7621 caacgaaact cgcaccggta ttattgatgt cattcgtgcc atgggtggaa aattggaaat
     7681 aactgaaatc gatccagtcg ctaaatcttc taccttgact gttgagtctt ctgacttgaa
     7741 aggaacagag attggtggcg ctttgattcc acgattgatt gatgaattgc ccattattgc
     7801 cctgcttgcg actcaagccc aaggtgtaac agttatcaag gatgctgagg agctcaaggt
     7861 caaggaaaca gaccgtattc aggttgtggc agacgcctta aatagtatgg gagcagatat
     7921 tactcctacg gcagatggga tgattatcaa agggaaatca gctcttcacg gtgctagagt
     7981 caatacgttt ggtgaccacc gtatcggcat gatgacagct atcgcagccc tcttggttgc
     8041 agatggagag gtggagcttg accgtgcaga agccatcaat accagctatc ctagtttctt
     8101 tgatgatttg gagagcttga ttcatggcta aggtgttatt agggtttatg ggggctggaa
     8161 aatcgactat tgcaagaggc ttggacccta attaccttga tatggatgct ctgattgaga
     8221 agcgcctagg tatgtccatt gcgaattttt tcgctgaaaa gggagaagca gcctttcgtc
     8281 aggtagaatc agaagtccta gctgatttac tacaaacaga ccaagtcgtg tcaactggag
     8341 gaggagtggt tatttctcag agaaatcgtg acttactcaa gactaataca gataacatct
     8401 acctgaaagc agattttgaa accctctacc tacgtatcgt agctgataag gacaatcagc
     8461 gaccgctttt tctaaataat agcaaggaag aactagtagc tatttttcaa gaaagacagg
     8521 cttggtatga ggaagtggct agtcgggttt tggatgtgac caagctaagc ccagaggaaa
     8581 ttatagagga actaagatga aaattgctta tctaggtccc aagggatcat tttcacacca
     8641 cgttgtgcag acagcttttc ctcatgagga attgcaggct tttgccaaca ttacagatgt
     8701 catcaaggct tatgagcaag gattagtgaa ctattctgtg gtgccagttg aaaattctat
     8761 tgagggtagt gttcatgaaa ccttggacta tctttttcat caggctcaca tccaagcagt
     8821 agcagaaatc gttcagccta ttcatcagca gttgatggtg gttccaggcc atactaagat
     8881 tgaaaagatt ttttcacatc cacaggcctt ggctcaagga aagaaattca tcgatgaaca
     8941 atatccagag gctcaaatcg aggtaacagc tagtacagct tatgcggccc gttttatttc
     9001 cgaacatcca gaccagccct ttgcagcagt tgcacctaga agttctgctg aagaatatgg
     9061 attggaactg attgccgagg atattcagga aatggaagcc aatttcacac gtttctgggt
     9121 tctaggagct gaaaatccta gtattccctt gcaagcacaa actgaaaaga tgagtttggc
     9181 cttgacatta cctgacaacc ttccaggtgc actttataag gccctgtcga cctttgcttg
     9241 gcgaggaatt gacttgacaa aaattgaaag tcgtccactc aagacagcac tgggtgaata
     9301 ctttttcatt atcgatgtgg attataccga taaggacttg gtccactttg cccaaaaaga
     9361 attagaagcg attggaatcc agtataaaat tctgggtgcc tatcctattt atccaatatc
     9421 agaccatgga aaggagagaa gatgagtaaa gaaaatccct taagtcatca tgagcagttg
     9481 cgttatgatt atttgctaaa aaatattcac tatctcaatg agagagaaaa aaatgagttt
     9541 gcctatttgc aagaaaagct aactcttgct aggggaaata gtagctctag cttggaacaa
     9601 gaaagagaag agcaggttga cttaccaagc tatgcgaacc ggagtcgctc acaatccaaa
     9661 tcacaagcac tctcttcccc tccaaaaaag aaaagacgga agctccgtct taaacgaatt
     9721 tttatggtga ttttttcact tttggtctgt gtggctttgg ccatggtatt catgtttttg
     9781 cgtggttacc aagatgctag cgcaaagaaa actgctgatg ctcgggcagc tcaagtagaa
     9841 gtctttaatg gtcaggacac tagagatgga gttaatattt taatcatggg tactgatggt
     9901 cgaatcggcc agaacagtgt tgagacacga actgactcta ttatggtatt aaatgtcggg
     9961 ggctcagata agaaaatgaa gctagtcagt ttcatgcgtg ataatttggt ctatatagat
    10021 ggttatagtc aagtgattaa cggtagaaaa cagacagata acaagttaaa cgtagcctac
    10081 gagttaggag aacaagaggg gcaaaaaggg gcagaaatgg ttcgccaagt cttgaaagat
    10141 aattttgact tggacattaa gtactacgcc ttggttgatt tccaggcctt tgccacagcg
    10201 attgacacgc ttttccctga tggggtgacg attgatgctc aattttcaac attgaatggg
    10261 cgtcccctaa cagaagctac agtcggagat gatttatacg ctaccgagac tgagtctcca
    10321 acccaaacca ttaaagtcgg aaaacagcag atgaatggct cgaccctgct caattatgct
    10381 cgtttccgtg atgatgatga ggcagattac ggccgtacaa aaagacagca acaagtttta
    10441 acagcaattt tagagcaaat taaagatccc actaaacttt tcactggttc tgaagctctt
    10501 ggaaaggttt ttgctatgac ctcaaccaac gtaccctata ctttcctcct aacaaacggt
    10561 ttatctgttt tggatggagc aaaaaatggt attgaaaaat tgacgattcc agaactgggt
    10621 gactgggtag atgcctatga tgtttatgga ggcttgggct tgctggttga tcaaaacaaa
    10681 tatcaaacca agctcgctca aatgggcttg agataagata atactcaatg aaaatcaaag
    10741 tgcaaactag gaagctagcc gcaggctgct aaaaacacag ttttgaggtt gtagataaga
    10801 ctgacgaagt cagttaccta tacctacggc aaggtgaagc tgacgtggtt tgaagagatt
    10861 ttcgaagagt ataagacaga aaaatcaaag tttgatagct atttggatag cagtcaggct
    10921 ttgatttttt acagtctgca atagattttc accccttgtt tgtggtataa tgaaacgata
    10981 cgatagaaag aataatgaac aatatgactg atttaaaagc aattcaggct cgtagtctgg
    11041 agatggctga atattttgtg gccttttgta aagaacatga tttgctctgt tatctctgtg
    11101 gcggaggcgc tattggtgcc cttcgaaaca agggatttat tccttgggac gacgacctag
    11161 acttttttat gcctcgtaaa gattatgaga aattagcaga attatggcct cgttatgcag
    11221 atgaacgtta tttcttgtca aagagtcaca aggattttgt tgatcgtaat ctttttatta
    11281 ccattcgtga caaggaaacc acctgtatca agccttatca gcaggatttg gatttgccac
    11341 atggtctggc cttggatgtt ttgcctttgg attattatcc gaaaaatcca gctgagcgga
    11401 aaaaacaggt tcgttgggcc ttgatttatt cactcttttg tgcgcaaact attccagaaa
    11461 agcatggtgc tctcatgaaa tggggaagtc gcattttact gggtttgact ccaaaatctc
    11521 tctgttatcg catctggaaa aaagctgaga aagaaatgac taagtatgat ttggctgatt
    11581 gtgatggcat tacagaatta tgctcaggtc ctggctacat gagaaacaag tacccaatca
    11641 catcttttga agacaatctt ttcttgccat ttgaaggaac agagatgcct attccaatcg
    11701 gctatgatgt ctatctcaga actgcttttg gggattatat gacgcctcca ccagcagaca
    11761 agcaggtacc gcatcatgat gctgtcatcg ctgatatgga taagtcttat acagaataca
    11821 agggagaata tggtggctaa gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga
    11881 ggtggtgcag agaagattct atcaaccatt gtttcaaatc tggatccaga aaagtatgat
    11941 attgatattc ttgaaatgga gcactttgac aagggatatg aatctgttcc aaagcatgta
    12001 cgcattttaa aatcccttca agattatcgc caaaccagat ggttacgagc ttttttgtgg
    12061 agaatgagaa tttattttcc aagactggct cgtcgtttgc ttgtaaaaga tgattatgat
    12121 gttgaagttt cttttaccat tatgaatcca ccactgttgt tctctaaaag aagagaagtc
    12181 aagaagatat cttggattca tggaagtatt gaagaacttc ttaaggatag ctctaaaaga
    12241 gaatcacata gaagccagtt ggatgctgcg aatacaattg tagggatttc aaaaaagacc
    12301 agcaattcta tcaaggaagt ttatccagat tatacttcta aattacagac aatctacaat
    12361 ggatatgatt ttcagactat tctagaaaaa tctcaagaga agatcgatat cgagattgct
    12421 cctcaaagta tctgtactat cggacggatt gaggaaaata agggttctga ccgtgtagtg
    12481 gaagtgatac gattattaca ccaagaggga aaaaactatc atctctattt tatcggggct
    12541 ggtgatatgg aagaggaact gaaaaaacga gtcaaagagt atgggattga ggactatgta
    12601 catttccttg gttatcaaaa aaatccttat cagtatctat ctcagacgaa agttcttttg
    12661 tctatgtcta aacaagaagg ttttcctgga gtgtatgtgg aggccttgag tctgggactc
    12721 ccttttatct ctacggacgt tggaggggct gaggaattat cccaagaagg acgatttgga
    12781 caaatcattg agagcaatca agaggcagct caggcgatta ctaattacat gacttctgcc
    12841 tcaaactttg atgtcgatga ggctagccaa ttcattcaac aatttacaat tacaaaacaa
    12901 atcgaacaag tagaaaaact attagaggag tagcatggaa actgcattaa ttagtgtgat
    12961 tgtgccagtc tataatgtgg cgcagtacct agaaaaatcg atagcttcca ttcagaagca
    13021 gacctatcaa aatctggaaa ttattcttgt tgatgatggt gcaacagatg aaagtggtcg
    13081 cttgtgtgat tcaatcgctg aacaagatga cagggtgtca gtgcttcata aaaagaacga
    13141 aggattgtcg caagcacgaa atgatgggat gaagcaggct cacggggatt atctgatttt
    13201 tattgactca gatgattata tccatccaga aatgattcag agcttatatg agcaattagt
    13261 tcaagaagat gcggatgttt cgagctgtgg tgtcatgaat gtctatgcta atgatgaaag
    13321 cccacagtca gccaatcagg atgactattt tgtctgtgat tctcaaacat ttctaaagga
    13381 atacctcata ggtgaaaaaa tacctgggac gatttgcaat aagctaatca agagacagat
    13441 tgcaactgac ctatcctttc ctaaggggtt gatttacgaa gatgcctatt accattttga
    13501 tttaatcaag ttggccaaga agtatgtggt taatactaaa ccctattatt actatttcca
    13561 tagaggggat agtattacga ccaaacccta tgcagagaag gatttagcct atattgatat
    13621 ctaccaaaag ttttataatg aagttgtgaa aaactatcct gacttgaaag aggtcgcttt
    13681 tttcagattg gcctatgccc acttctttat tctggataag atgttgctag atgatcagta
    13741 taaacagttt gaagcctatt ctcagattca tcgtttttta aaaggccatg cctttgctat
    13801 ttctaggaat ccaattttcc gtaaggggag aagaattagt gctttggccc tattcataaa
    13861 tatttcctta tatcgattct tattactgaa aaatattgaa aaatctaaaa aattacatta
    13921 gaacgggggt gaccaagtga ttgatgggaa acgattatta tttagtttga ccatagtcag
    13981 ttatgccttg acgctagtaa gtggaattgt gtatctgttt aataataata atgttagctt
    14041 actttctact ttattgttct tgttggttag tagcttgatt gcttgttgga atgatatcaa
    14101 gtattactta atccatttta ttttctattt aaccattttt gtatttctgg tatcaagacc
    14161 gaccattgat tattttaggg atggtgcttt ggatacctat catccaatag cctatcgttt
    14221 tgcctttata gttgtcatgg tttcgattct gggcttgacc acaggaggca ttctggctcg
    14281 ttacttcata gctaggaaga aaataaaagt agcaaatata ggaaattctc taaaagaggt
    14341 ttatatcaag cggttacgct ttgtatcact aggagttttt cttctaacct atcctttcta
    14401 tttcattcgg ttatttgaac ggctcttgta tcgtttgcag acttcctact atgcctacta
    14461 tgcaaatttt gaaagtaaac tgccttattt tacctacatt ttgtctacct ttacggtcta
    14521 tgcaatgtgt atgtatctgg caaccaagcc aaagaaattg caggccacag cagtgcttgt
    14581 ctcctttatt gcagctaata ctattcattt ggcaattggg acacgaaatc cctttatttt
    14641 aagtatttta tttgcttttg tttattactt tatgcgggag caaactgaaa aaggaaaatg
    14701 gattgggttt aaagaaaagt tagcgatttt tgtaggttct cctattctca tgttagcgat
    14761 gggagtactt aattatgtac gggataatgt ccaagtttcc catacaggtt tctgggatat
    14821 cttacttgac tttatctata aacaaggaac tagttttggt gttctggctc gaggttttct
    14881 atttaacagt agcctccctt accgagattt ccgtaatttt acttttggtc ctgttcttga
    14941 ttattttgca agggggagtt tgggagccat tttcggagga aaagcctttg aacatacaac
    15001 caatagtgtg gaactagcta ttgatagtaa tagttatgcc cacaatctat cctatcttgt
    15061 cttgaacaag gaatacttga aagggcatgg tatcggaagt agttatatca tggagttgta
    15121 taccgactat ggtatgattg gagtctttct gcttagtttc ttactcggcg tattatttat
    15181 agccatgctg caagtagcct atcgctcaag gacaatccta tttgctttat ccctactcat
    15241 cttgaataat ctattcttta tgccaagaag cagcttttca gaaagtttct tcaatttatt
    15301 tacaatgcaa ttctggggaa ttgttcttgt gattatattt gtagcaaaaa tgcttacaaa
    15361 ggaaaactag tatctactaa acaaaggaga aaaaaatcat gtttgaacat tattcagtag
    15421 ctgatttgtt tgcaaatctt tataaaaagc gaaaagcaaa tattttagct ctcatcgctt
    15481 tatttgctct cattgctgta ccatttacaa ttaaagcagt taggaataaa aacactgtca
    15541 aagatacaac aagttattca acttatctta tctataaaat cactcctcca aaagagtcgg
    15601 ccaaaacgat tttgaatcat caaattggtg gttatagtga tttttatggg aaattgattg
    15661 atggtaattt gaatggagct tatcttttca atgatgtaga acccagtgag ttgaaaaaaa
    15721 ttgccagtga attagacacg acagaaacaa ccttgaaaaa ttcaacgaat gactattggt
    15781 ggaaaaaatt gaccgtctac tatatgattg acgatgcagg ggttggtgtg aaaattttga
    15841 catcaagtaa agatgccaat gacttgttag agaaaaaaat tgatgggttg attgagaaat
    15901 ttaaacatgc ctatgcaaat gtgaaaattg aaaaactgga aaccatcaac tctaaagaat
    15961 tgaacgcaaa tggtgaaaca gcgcttggct taaatgtgaa aaatctgatt cttcgtttag
    16021 ttgttattgg agtggtttgt gtgattttgg ttgtgatggg aaatgtgtta gtttatctct
    16081 ttaatccaac aatcaataga gtaggtgatt tttctcagta tcaaattgat tttgtaacag
    16141 agattacaac aattgctaac ctagcagatg ttttgtcata caaaaatact ggacaggaat
    16201 tgaccatcgt tagctcaaat aaagctatcc tagataaatt gaaacagagt caagaagctt
    16261 taaaaggaat gcattttgta gatttacagg atgtatcatc tcttttggaa agagatacag
    16321 tccttcttgt tgaagagtac ggagtgactc gttataagaa atttgagcaa agtcttcaaa
    16381 ttctcagaaa cttaaatcgt tctatccttg gtgtagcaac ctttaaatta taagtattct
    16441 gatgtataag gtcttcaaaa tcggctaaga gctgattttg aaggcttttt ctatctgtct
    16501 gcagaaattt tcttttggag tctcctatag aaagacccta gggaaaatct atccgtatag
    16561 aggagccttc ttttgataaa atcgtaaaaa tctagtataa tagatagaac tgaaagtatg
    16621 aggttactag caatgaaaat gaaacaaatt agtgatacaa ctttaaaaat cacgatgtct
    16681 ttagaggatt tgatggatcg tggaatggag attgctgact ttctcgttcc tcaagaaaaa
    16741 acagaagagt tcttttatgc tatcttggat gaactagaga tgcctgatag ctttctggat
    16801 acaggcatgt tgagcttccg tgtgactcca aaacctgata aggtagatgt ctttgtgacc
    16861 aagtcaaaga ttgaccaaaa tctagatttt gaagacttat cggatttgcc agatatggaa
    16921 gaattggctc aaatgtctcc agatgaattt atcaaaaccc tggaaaaaag catcgcagac
    16981 aaaaccaagg atgatatcga agccattcaa tctcttgagc aagttgaagc caaggaagaa
    17041 gagcaagagc aggctgaaca agaagctgag agtaagaaag aaccttacat ctactacatc
    17101 ctttcttttg ctaagttggc tgacttggta gtttttgcca agacagtgac ttttgaaatg
    17161 gaaacttctg aactctacaa aatgaacgag cgctattatt tgaccatttt agtggatatt
    17221 gaaaatcatc caagcccata tccagcttgg ctgttggccc gtatgcgcga gtttgcagac
    17281 gatagtgata tcagtcgctc agtcttacaa gagtatggtc aagtcttgat gagtcacgat
    17341 gcagtgctca atctgcaaaa aatcggctaa tcctttctgg aaagctattt tcagatttta
    17401 agaagagcga atcgtctgat tcgttttt
//
LOCUS       DAVLTM010000034        17146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLTM010000034 DAVLTM010000000
VERSION     DAVLTM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.034"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(97..660)
                     /locus_tag="VYN16_000974"
     CDS             complement(97..660)
                     /locus_tag="VYN16_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1403524.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILSLAIVPRLQTLKK"
     gene            complement(644..1195)
                     /gene="coaC"
                     /locus_tag="VYN16_000975"
     CDS             complement(644..1195)
                     /gene="coaC"
                     /locus_tag="VYN16_000975"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1403525.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLSLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(1207..1896)
                     /gene="coaB"
                     /locus_tag="VYN16_000976"
     CDS             complement(1207..1896)
                     /gene="coaB"
                     /locus_tag="VYN16_000976"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1403526.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPESHPNLSIREITNTKDLLIEMQERVQDYQILIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2126..3796
                     /locus_tag="VYN16_000977"
     CDS             2126..3796
                     /locus_tag="VYN16_000977"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1403527.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKLR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(4287..5453)
                     /locus_tag="VYN16_000978"
     CDS             complement(4287..5453)
                     /locus_tag="VYN16_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1403528.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLKTEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFLASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(5466..6205)
                     /locus_tag="VYN16_000979"
                     /pseudo
     CDS             complement(5466..6205)
                     /locus_tag="VYN16_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(6227..7051)
                     /locus_tag="VYN16_000980"
     CDS             complement(6227..7051)
                     /locus_tag="VYN16_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1403529.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISHMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            complement(7188..8402)
                     /gene="thiI"
                     /locus_tag="VYN16_000981"
     CDS             complement(7188..8402)
                     /gene="thiI"
                     /locus_tag="VYN16_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1403530.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYAQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(8411..9553)
                     /locus_tag="VYN16_000982"
     CDS             complement(8411..9553)
                     /locus_tag="VYN16_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1403531.1"
                     /translation="MIYFDNSATTKPYPEALETYVQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFSPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            9691..10125
                     /locus_tag="VYN16_000983"
     CDS             9691..10125
                     /locus_tag="VYN16_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403532.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            complement(10170..12395)
                     /locus_tag="VYN16_000984"
     CDS             complement(10170..12395)
                     /locus_tag="VYN16_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1403533.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(12559..14511)
                     /locus_tag="VYN16_000985"
     CDS             complement(12559..14511)
                     /locus_tag="VYN16_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403534.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGLIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(14508..15419)
                     /gene="pfkB"
                     /locus_tag="VYN16_000986"
     CDS             complement(14508..15419)
                     /gene="pfkB"
                     /locus_tag="VYN16_000986"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1403535.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(15416..16156)
                     /locus_tag="VYN16_000987"
     CDS             complement(15416..16156)
                     /locus_tag="VYN16_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1403536.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            16318..16405
                     /locus_tag="VYN16_000988"
     tRNA            16318..16405
                     /locus_tag="VYN16_000988"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16352..16354,aa:Ser,seq:gga)
     gene            complement(16600..16947)
                     /gene="ssrA"
                     /locus_tag="VYN16_000989"
     tmRNA           complement(16600..16947)
                     /gene="ssrA"
                     /locus_tag="VYN16_000989"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         5023 a         3689 c         3400 g         5034 t
ORIGIN      
        1 ctgttcttat ttcgttttac tatagtaaag atttcattaa aaagaaactg tatagagcaa
       61 aatctccacc ttcaggtttg aaagcggaga ttgtttttat tttttcaagg tttgtagtcg
      121 tggaacaatg gctagggata gaattgcaga aatgaccaat tcagcaattg aatttgttga
      181 gataacggtt gccagaagaa gttggatatt tccattataa acatttccaa ataggaagaa
      241 gattcctcca aggacaaaga tagtatttgt caaggaacca agggccccag ctaaaatcag
      301 accagtcttg tttttcatca gtttatagac taagtaagga gttaaaccaa tcaaaatacg
      361 tgggacaatg gcaatgatag ctgagtagat gtttccgttt ggtacgaagg gagagaagag
      421 gtagcttgtc ggtagaatcg taatcgtgtt aaccgtcaag ctaagtaatc ccatcaaaaa
      481 tccaagtgta accccaactc gtggaccata aataatgctg gcaataatga caggaatatg
      541 aacaatggtc ggtttgattg gaaatggaaa aaggttaaag ataagtgagc tcagaaagtg
      601 tatcacgagc atggtagcaa aaaagatagc aatgggtgca atattagagc gttttttcat
      661 cgatagtttc ctttattctt tctaaaataa ttgtgaggtc agctaaagct cctcgtccgt
      721 ggtctccaca agctagtagg gattccttag gagcaatcag ctgatagccg taggtttcta
      781 atgttttcag attattctga gttactggat ggtcatacat ttttgtattc atagcaggag
      841 ctattagttt gggaatatga cttggtaggg ctagagctgt actggttacc atgttgtccg
      901 caaagccgtg agctagtttt gcaatagtgt tagcagttgc aggtaccacg ataaataaat
      961 ctgctttttt tccaagttcg atatgattga cctgatcagg atagggttcc ttcatgacat
     1021 ccaagtggac aggattctgt gagagtacct gtagtgacaa aggttggata aactctgtag
     1081 cagcctgagt cattaagaca gtgacttgat ggccttgttt ttttagagaa ctgactaaat
     1141 ctgccgactt ataagaggcg attgaacccg ttacagccaa gagaatgttt gccatagttt
     1201 tcctttctaa gaatgatagg cttgaatttt ttcaaggagg agttctgcaa tttcttcttt
     1261 agtctggact gtttgaagct gatttttctc aacaaatata gctcggtgct gatctgctga
     1321 aatttgagtc aggtcattcg cgatgattaa atctgcttga ttcttgataa gactttttcg
     1381 tgcaatatca accagatgat cttcggtaac atcaaccagc agtttgaaac caatcagatg
     1441 aatagtagga ttccattcct tgactaggga tatgattttg ggtgtctttt taaggaacaa
     1501 aacctgaacc tcatcagttg aagaaatctt ggcctgatga ttctgcttgc ttaaaaattc
     1561 ttttagattg gagctagcct gaacttcctc aagccctgtc atataaacag gagtgtagtc
     1621 agaaacagcc attgagtgga tcaagatctg ataatcctga acacgttctt gcatttctat
     1681 tagaaggtcc ttggtattgg taatttctcg aatacttagg ttaggatgag actctggctt
     1741 cagagctcgt tttgtcgtaa ttaaacaaac ttcatgccct gcagaaagca aagtttctgt
     1801 gataattttc cccaagtgac ctgtagaatg gttagtgata gagcggacgc tatcgatagc
     1861 ttcactggta ccgcccgatg taactaaaat tttcatagca ctattgtaca caaaaataag
     1921 cggtaagtaa aatgaaagtg ataaattttt ggtaaaaacg aagatttatt atagtttcaa
     1981 actataaagc catataaaat ttaagaaagg actctaaaag tcttaaaaac agagatattt
     2041 acgaacgttt agagagtttt aggatgttaa aaatcttgtt ttgtgatata atgaattatc
     2101 atataagagg ttagaggaat tttgaatgaa aacagatatt gaaatcgcac agagtattga
     2161 gttgaagcca attgttgatg ttgtagagaa acttggtatt tcttacgacg atttggagtt
     2221 gtacggaaag tacaaggcta aactcagctt tgataaaatt cgggcagttg agagcaatcc
     2281 agtcggtaaa ttgatcttgg ttactgccat caacccaaca cctgcaggtg aaggaaagtc
     2341 gacgcttacc attggtcttg cggatgcctt gaacaagatt ggcaagaaaa ccatgattgc
     2401 tatccgcgaa ccgtctcttg gtccagtcat ggggatcaag ggtggtgctg ctggtggtgg
     2461 gtatgcacaa gttctgccaa tggaagacat caacctccac tttactggag atatgcatgc
     2521 tattacaact gccaacaatg ccctttctgc cttgattgac aaccacttgc accaagggaa
     2581 tgagctggga attgatcaac gtcgtatcct ctggaaacgc gttgtggact tgaacgaccg
     2641 tgcgctccgc catgtgactg ttggtcttgg tggtcctcta aacggtattc cacgtgagga
     2701 cggttttgat attacagttg cttcagaaat catggcaatt ctttgcttgg caacggacat
     2761 cgaggacttg aaacttcgtt tggcgaatat cgttattggt tatcgctatg accgtacgcc
     2821 tgtttctgta ggtgatttgc aggttgaggg tgccttggct ttgattttga aggatgctat
     2881 taagccaaac ttggttcaga caatttacgg tacacctgcc tttgtacacg gtggtccatt
     2941 tgccaatatc gctcatggct gtaactctgt tttggcgacg acaacagccc ttcacttggc
     3001 tgattacact gttactgaag ctggttttgg tgcggacctt ggtgctgaga aattccttga
     3061 tatcaagaca ccaaacttgc caacatctcc agatgcagtt gttattgtcg caacccttcg
     3121 tgcccttaag atgaatggtg gtgtggctaa agacgctctg actgaagaaa atgtagaggc
     3181 agttcgtgca ggttttgcta acttgaaacg ccacgttgaa aatatccgta agttcggtat
     3241 tccagcagtt gtagctatta acgaatttgt atctgataca gaagctgaaa ttgcagtctt
     3301 aaaagaactc tgtgcctcaa tcgatgtacc agttgaattg gctagtgtct gggctgatgg
     3361 agcagaaggt ggagtagcac ttgccgaaac agttgttaag acaattgctg aaaatccagc
     3421 taactataaa cgtttgtatg ataatgacct ttctgtccaa gaaaagattg aaaaaattgt
     3481 cactgaaatc tatcgtggta gcaaagtgaa ctttgagaag aaatctcaaa cacaaattgc
     3541 tcaaatcgtt caaaacggtt gggacaaatt gccaatctgt atggctaaaa ctcaatacag
     3601 tttctcagac aatccaaatg cgcttggagc accagaaaac tttgaaatta ccattcgtga
     3661 attggtacca aaattaggtg caggcttcat cgttgcctta actggtgatg ttatgaccat
     3721 gccaggtctt ccaaaacgtc cagcagctct caacatggat gttgaaagcg atggaactgt
     3781 actaggcttg ttctagtgga ttgaaattga ctttaagtga gtttggaaat aatatccaag
     3841 ctcatttttt ctcagactgc aagcagttca tcaataagat ttatactctt cgaaaatctc
     3901 ttcaaactac gtcagcttca ccttgccgta ggtatatgat actgactctg tcagttctat
     3961 ccacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga
     4021 ttttcattga gtattaatag actggtttgg tatcctgaaa actatatcgt ataggtgtcg
     4081 tcatggatcc ttttagctga tttaaggata cgaaaacaca atactatgac taaattgaaa
     4141 tgttaaaatt caatttctag ccacctaatc ctgcttcaaa tagttatcga acaagtttta
     4201 tgatagctac cttgttagga tgctcttatt ttttaccaga caaaacaaaa ggattaaggg
     4261 agtgaatttc cttaatccga tagttcttat tcttgacgtt gtccgaaatc agcaatcatc
     4321 tgatccattt cttgtcgact cggagttgtc agcatataca ggtaatctcc ttgaagttcc
     4381 gcgaaggcag ctggcatgac ttttttaacc ttgaactgct ggctatattt ggcaagcaaa
     4441 tcctcttctg aataaacata gtgagcattt gcacggcgag aagtagccaa acaatactga
     4501 gtttcaaagt ctgacgccag gaactcctct cctgcgacaa tagctgcata gccacgataa
     4561 aggtccaagg aatgagcaaa gttataaaca tcaatggtaa aaccacctgc agggcggtta
     4621 ttgtactcga tggtaatata atcgtccccc tcacggaaga actcaatatg gaaaaaccgt
     4681 tctttcatac caaattcttt gacaattgct tccccatact tgcgcagttt aggatccata
     4741 tccttgagca cataataaga attgtccatc ttataaatca tgaggtcaag cggtgtatag
     4801 gcgtagtcaa aggttgttga gaagacaatc tttccatcct tgtccacgag cccgtcaaag
     4861 gtacagattt cgctggaagt gacaaatttt tcaaagaaat aaagggttga atggtcccat
     4921 tcttgcttga agtgattgat atcgtcttct gtcttaagtt taaaggttgc ggctgctccc
     4981 actccattat caggtttggc aatcattgga agaccgattt ctttcactgc ttgatcaaca
     5041 tctgcttccg tcttgataac agctccaggt accacaggaa cacctgcttt tttgaaaagt
     5101 ttcttcattt cagacttata tttcgtcttt ttgagatcct ctggtttggc accaaaaaca
     5161 ttgaattgtt ctctgagtgt tgcgtctagc tcaagccagt attcattgtg agactcgatg
     5221 cggtcaattg gaccatgttt ataaaagaga aaagcaactg cacgtttgac ttcatctatg
     5281 ttctcaagat tatcaacacg aaaatactcg gtcaagctat tgcgcaaggg ctcatccaat
     5341 tgctcgtaag actcttgacc aattcccaag actgtgatgc ctttattagc tagttcgatg
     5401 gtaaactgtt gaaagttttg tggatagtag ggagaaataa caaggtaatt cataggtaac
     5461 tccttttata aatagagatt accgaggaaa taaggcattt gtttacgcca ccattcccag
     5521 tcatgggcga catcatgtcc ccattcagca aaccaggctg gaatttgttt cttgtcaaag
     5581 gcttctttga gcttgtaaaa ggatggcaaa ccatcttgtt cccaggctcc aagccccgta
     5641 cacagcacaa tctctgcctg acggtaacgg tcaataaacc agccgtcgtt ttggttccaa
     5701 atataatcta ctggcgagtt ttggtaaata gcatcatcgt tgtagtaatc accgacaaag
     5761 aaacgtgcgt cgtaaacacc actgagagca atcactttgg taaagacatc tggatgctgg
     5821 aggaagaaat tgagtgcatg ataggctccc atagagcaac ctgtcgtcat catgccatca
     5881 aaccaacctg tcttgtgctt gataaaagaa tggcctcctc aatcacataa cattcgtagg
     5941 cacggtgcat ttccgcttgg tcatgagcat ttttccaagt agccaaccag ctctcactat
     6001 ccacactaga tagggtaaag aactagacaa ggccttcctc gataaaggaa gcacaggcat
     6061 caatcatgcc aaaatcatag tattcgttgt gactaccacc tgatgaagca aagaccacaa
     6121 ctggaatccc accatgtcca taacggttaa ggtacatttc acggttagga tgaccactcc
     6181 agtggctaag atgttcaata tgcatttcgt tttttccttt cttaacttac catttttctg
     6241 caaaaaatct cagacaatct ggtagatttt ctgaccaagg gatttcacta tggatggcac
     6301 cagactgaac ttttagcaca agattatcca gatgtactcc ccctgctatc aaatcatggt
     6361 aatagcaaag cgacgagtcg atataggctt gtttgatatt gccatccatc aaggtcttgt
     6421 ctgtatcatc tgcttcttct gttcctacat agatgaagat gcgctggtca ggcgatagtt
     6481 tctggcactc gatatagcgg ttaaaggctt cttggtggag ccagtttgca gatgaaaaaa
     6541 cgcccaagca accaatttgg tcttggtatt ccaaaccgat aaactgggta atattgcctc
     6601 ctagtgagga accaatcata gccgtatgct ggcagtctgc ttttgtacga taggtctcat
     6661 cgataaaagg cttgaccacc tccatgacaa actcagcata ctccacaccc ttaccaccaa
     6721 actgctgctc tgggatagga gattcttgga acttccaagc cgcatactca ttcattcgcc
     6781 ccataccatc attgtcaata gcaacgacaa tcatgtgact gatatccgga tttcgtttga
     6841 tagctgggat aatcttccat gaatatccaa tgaaagactc tttgctataa aaaacatttt
     6901 gcccgtcatg aaagtataca acaggatagg aacggtctgt atctttctca taatctttag
     6961 gaagaagaat acgtacacgg cgctccttac ctgtataagg aaccttgagt ttgtgttctt
     7021 tcatttttag ataaaagtag gattgattca ttgtcagaaa actctctata ttcaaaattt
     7081 tatatcatta tatcatgaaa atagaaaaaa gttagttttt tacctgtttt tagaaaaaaa
     7141 aactaacttt ttttactgat tttcgctatt cttttggatt ttctgaatta gagtagatta
     7201 tcaatcaagt catcaacttc atctttttcg gcttgaggtg tgatttcagt aatcatgatt
     7261 ccagccactg ctcgctcaac caagccttca acatccatac gcgcttcgta ctgctccgca
     7321 ttcttaattt taggatttgt ttttggacga tctggtgcaa aaatggtaca acagtcttca
     7381 aacggttgga ttgaaatgtc aaaggtatcg atttcctggg cgatgtcaat gatttccaac
     7441 ttgtccatgg taaccacagg acgaatgatg ggagtgttgg taacagcatt gatagccttc
     7501 atactttcaa gggtttggct ggctacttga cctagacttt ccccattgat gataaccaaa
     7561 ccatttcgta cctcacgaat acggtcagta atccgcatca taaagcgacg agttagagtc
     7621 atcaaataag cttctggcgc tttggctttg atttcctctt gaatctctgt gaaaggcacc
     7681 tcgataaact ggatatttcc gccaaacttg gtcaatttac gtgtcaagtc ctgcgctttc
     7741 ttgagggcac caggactagt atatggtgga ctagcaaagt gaactgcctc gatatccacc
     7801 ccacgcttaa gagcaagata acctgctaca ggtgagtcaa tccctcctga caacatgagc
     7861 atccctttac ctgaagttcc aactggcaaa ccaccagccc cacgaatggt ttcataagaa
     7921 agataggctg cttcttcacg aatctccacc tgaagattga tgtcaggact tttcatttga
     7981 acttgcacat ttggaatggc ttcgaataca gcccctccaa gtgtttggtt gagttcacga
     8041 ctatcaagtt caaagttgtg gtcgctacgc ttgctagaaa tcttaaaggt cataccttcc
     8101 ttgtagatgt cccgcataat ctcttggaca gaagacttca aaacttctac agatttttca
     8161 accttataaa caggagaaaa gttttgaatt ccaaaaactt gtttgagaga ttctgcaact
     8221 gctgtgtaat cagctccatt gaggtaagcg tgggcacggt cgcgatctgc tgttacctta
     8281 acttgggcat agatagacaa aacgtccgaa atattattac gaagtttatt gatgaaacgc
     8341 atacggtttt tacccttggt tgacaactct ccgtagcgaa tcataatttc tgaatactgc
     8401 atgaatgctc ctatcttact tttctagttt gattgtaaat caattttaac ttggtcaaaa
     8461 actgctcgac ctgactcata tcattttcca agtctaggct aagacgcaca gctgacttgg
     8521 ccttatcttt gtccactccc atggcaatca aggtaccggc tggttttcct gccttagatg
     8581 aacaagctga ggttgttgag atgaaaatat catagtcttc aaaggcgtga acgatgactt
     8641 cacctcgaac acctttgatt ccaaaagtca gaatatgagg tgcaaagtct tcctcatctg
     8701 aaaagacaaa aatatccgga tagttcagaa gagcttggtg aatcactgcc ttcatctgcc
     8761 cagtcttgct cctaaagata tctagctttt ccatagataa acggagagcc ttggctgtcg
     8821 ctgcaatccc tgccacattt tcagttgtcg aacgataatc tcgctcctgg ccaccacctg
     8881 taagaagagg tgtaatcttc ttgccagatt tgatatagac aaagccaaca ccacggacac
     8941 catgaaattt atgactcgag aaggtcgcgc aatccacccg ttctgtcaga tacttttcag
     9001 tcggaatttt ggcaagcgcc tgaaccgcat caacgtggaa ggaaatagtc ggcttgtctg
     9061 ccaagaattc tgaaatagcc tcaataggtt ggatagagcc gatttcattg ttcacagcca
     9121 tgatggaaac gaggattgta tcaggccgta tcaaacctgc taacgcctca acatcgacca
     9181 agcctttctt atcaactgga gaaaaatcca cttcaaatcc ttgacttttc aaccagaggg
     9241 ctgactcttt gactgctgga tgttcaatgg ctgaaacaat gatgtgcttg ccaaactgag
     9301 ctttttcaaa ggccacaccc ttgataaccc agttatcccc ttctgttcca ccagaggtaa
     9361 agaagatttc atcgcttttc ttaccgatta aatctgcaat ctgttggcga gaagcatcta
     9421 agattcgtgt tgcttggtct cccaaacgat ggagactaga tgggtttcct aaaatttttg
     9481 aagcgacctg cacataggtt tcaagggctt caggataagg cttggtcgtc gctgaattat
     9541 caaagtaaat catgtttctc acgctttcta aaatcactcc ctctattgta tcatgaaacg
     9601 gagcttgcga caagaaaggg caatttctct ttttttaaga tttttttaaa gaaacagagt
     9661 ataataagtc ataatcaaag gagagaatgt atgtctaatt atcgtagaac ttcaaaacca
     9721 aaaacagaac acatcaaaaa aggctttaca gtctttcaaa aaaccgttgc gactatcgct
     9781 agtatccttg gcttgattac cgctagtatc acgattatga acgccttgga taataacaaa
     9841 aatactaaaa aggaacctac aacaagccag acgacaacaa ttgtcaaaga aattcaaaag
     9901 gaatcccctc aggaaaatac tagtcccaat aaggaaaata agacttctca agaaaaaaca
     9961 caacaagaag aaacgccaaa atccagcgtc aaggaagaga aaaaagaaga gcagaaaaca
    10021 tcaactcagg attcttctac tcctgctcct actccaagta aacctgctac tgaaaacgaa
    10081 aaacagtcca ataccccaac ttcggaaaat aaaactaatc aataatcact aaaatagcct
    10141 ccttccaaac ttggaaagaa gctatttttt tattgttgta aaacttttct tggtttggta
    10201 ccttcagctg gaccaatgac acctgccatc tcaagctctt ccatgagacg ggtcgcacgg
    10261 ttaaatccaa ctgacaaacg acgctgaatc atggatgcac tggctttctg tgtttcgata
    10321 accaaagact tagcttcttc aaaaagcgga tcaccaccag catctccatc cgaaaattct
    10381 ccttcatttt cagaaacctc acctggatca aaactctcat cgtagtctgc atctgcctga
    10441 gtcttgatga agttcacaat gcgctcaaca tcgtcatccg agataaagga gccttggaga
    10501 cgaactggat gattttcatc aatcggttta aagagcatgt ctcctcgacc aagaagtttt
    10561 tctgctccat tttcatccaa aatcgtacgg gagtctgttc ctgatgaaac cgcaaatgct
    10621 acacgagatg gaacattggc cttaatcaaa ccagagatga catcaacaga tggacgctga
    10681 gttgcaagaa tcatgtggat acctgcagca cgcgccttct gcccaagacg gatgatagca
    10741 tcttccactt ccttgctggc caccatcatg aggtcagcca actcatccac aatcacgaca
    10801 atgaatggta gcggaatttg cttgtactca gactgggaat tgaactcttc taccttggca
    10861 ttaaaacctg caatattccg aactcccacc ttggcaaaga gttcataacg gttttccatt
    10921 tcatccacaa ccttttgcag agccttgctg gctttacgtg gattggtcac gactggaatc
    10981 aagaggtggg gaatatcatt gtaaacagat aactcaacca tcttgggatc gaccatcata
    11041 aatttaactt gatctggtct cgccttcatg agaatgctag caataatgcc gttaactgct
    11101 actgacttcc ctgaacccgt tgaacctgca actagcaagt ggggcatttt agaaaggtca
    11161 aaagctcttg cggttccatt aacagccttc cctaaaggaa tttccaagaa attttctgct
    11221 ttcgtttgcg attgttccca tagttctcgg aaagatacag tggcaatatc ggagttgggc
    11281 acttcaattc cgattaggga tttcccaggg attggtgctt caatccggac atctttggca
    11341 gccaaggcta gagcgaggtc atctgataga ttggaaatgc ggttgaccct tacaccaaca
    11401 gccggcttga cttcatactt ggtcactgat ggcccaattt cggcccgttc aactgttacc
    11461 ttaataccaa agctagcaaa ggttgcttct aagattttga tattttctct gacaattttc
    11521 ttctctttag actgatcttt tggtttatct ggtgcaaaga gttgtaagct tggaagtttg
    11581 tattcaaggg cttctttggc tgaaaaatcg acctgaacat cttggtcatc tgagtcatct
    11641 tcctgttcag ggaatttaag ttcagcttga ggcaggatga tttctggttc cacccacttt
    11701 tcttctggaa taggtggaag attttgaaca gcttcctctg tcagaatttc acccgtttcc
    11761 atatcaacag gaggcaaatc gagtaaggct ttttcagtct cttcttgttc taatctagcc
    11821 tctttctcag ccttttggcg agctttttct tcttgtttga caaagcgttc ctcttttcga
    11881 cgctcgtgcc cttcccacca tttggcaaag cctctactga aaaattcagc aatatcgtaa
    11941 acagaccaag ggctgactag gagagaaccc actaaaatca agatagaacc aataaagtaa
    12001 gttccgatat ttgaaaagag aaaggctgtt ggaatataaa gagcgacccc gatcaagccc
    12061 cctccagcaa agctagtcgt tcgaaaacca gtcagatctg tcacaacctg agccatggtc
    12121 ccttttagaa cggacttgtc caaaccatat ttccaaacta agtaggcctc aaaaatcaag
    12181 agtaagccag caaatatggt gaaaaagcca gataagagtc cttcctgttt tcgtatccac
    12241 ttgaaaaaga agagatagat taataggccg aatatcgcca gataagctag gctacccact
    12301 agcaagcgaa ttaaattata aagggttata cctgcagccc ctaatttgaa ggctgcgaaa
    12361 atcaataaaa tcgcaattcc taatgaaatc aacattcgtt gaatcgcttc ttttctttcc
    12421 agttctgctt tagacggtct ccgtcttgtt gtacttgtat tcttgtttgc cattcttcta
    12481 ttataccata tttcacaggc atttcgggaa gagaaaaaga ctgcaccaaa cggtccaatc
    12541 ttttcatttt tctatttttt atgcttgtgg tttgcgtagg taaccataaa ccacaccact
    12601 tacgattgct cctaccaaga cagaaacgag gtaaaggaga gcatttgaag taagggcgat
    12661 aacgaagatt cctccgtgtg gcgccatgag tttgatacca gtaagaccaa cgagtccacc
    12721 tgctactgct gaaccaagga tgaagcttgg aatcgcacga gctgggtcag cggcaccaaa
    12781 tggaatcgct ccctcagtga taaatgacaa gcccatgatg atgtttgtca aaccagagtt
    12841 acgttcttcc ttagtaaatt tatctttgaa aagaagagtt gcgacaaaga ttgcaagtgg
    12901 tggcaccatt cctccagcca taactgctgc catggctaca gaaccacctg aagaaacagt
    12961 tgctgcaagc gtacctgtac caaagacata agctgcttta ttaactggtc cacccatgtc
    13021 aacagccatc attccaccaa ggacgatacc aagaaggaca gctgaacctc ctccaagacc
    13081 gcctaggaag tcattcatag cagtgttgat tgcagccatt gggatattca cagctagcat
    13141 aacaaatcct gtcaagattg ttccaagaag tggcaataga aggattgatt tagcaccttc
    13201 gagtgaacga ggaactttaa cgtatttctt gatggcaaga accaaggcac ctgcgataag
    13261 tccaccaaca agggcaccta ggaaaccaga tgagacacct gcaagagttg aagttgcttc
    13321 accacctgcg gcataaggaa ttttaccaaa ggcaaaacct tctttggcaa tagcaccagc
    13381 cacgaaacct gctaccaaac ccggtttttc agcaatagag taggcaacat aacccgcaaa
    13441 gactggaagc atcaaaccaa aggcagctcc accaattttc atgaacatag aagctaactc
    13501 atggtaagaa ccaagattgc caaggttttc atttggaaca cccaaagcac cgtcaatcaa
    13561 gaaggcaagg gcaatcatga taccaccacc gataacgaat ggtaacattt gagatacacc
    13621 actcatcaag tgtttgtaga aggcaccacc aaggctttgt ttttcgttag aggctgttgc
    13681 agctttggca ccattagcgg cacggtagac ttcagtatct cctgaaagag ccaagttaat
    13741 tagctcttct gtcttacgga taccgtcagc aactggacga ttgatcaatg gttttccatc
    13801 aaatcgatcc atttcaacgg ccttgtctgc tgcaatgata atagctttag ccttacggat
    13861 atcttctgca gttagttgat ttccaacacc gctagcaccg ttggtttcga ccttgatacc
    13921 aacccccatt tcagcagcta ctttttgaag ggcttcttgg gccatgtaag tgtgggcaat
    13981 acctgttgta caagctgtaa cagctacgat aaagtcacca gagtcattag caggtgcttg
    14041 aacaagttcc tcagtttttt ctgaagcttg gtcaaaaagt tcgataactt ggtctgcaga
    14101 tgttgcttga cgaagtttgt ctgcaaaacc gtctttcatc aagtattgag acaattctgc
    14161 caaggctgcc aagtgagtat cattggcacc ttctggagct gcaatcatga agaagaggtc
    14221 agttgcttgt ccatccaagc tctcgtagtc aacaccctta tttgacttag caaatagaac
    14281 tgtcgcttct ttgacagcag cgtttttgct gtgaggcatt gcgattccat cacccaaacc
    14341 agtagaagtc aaagcttcac gcgccaaaat tccttcttta aatgtttcaa aatctgttac
    14401 ataaccgtgc tctgtcaaat ttttaatcat ctcgtcgatg acagctgttt tttcagttgc
    14461 ctgcaaatct agcaacatga catcttttct caataggtct tgaattttca tcttttttct
    14521 acctcaactt ttccatatgt ttctttaata aattccgccg ttgccaagtc atctgagaag
    14581 gtagttgccg ttccgcaagc cactccccat ttgaaggctt ctactacgtc ttttgatttg
    14641 acaaattcac ctgtgaatcc agcaaccata gaatcaccag ctccaactga atttttgact
    14701 gttcctttga ttggtttagc gaagtaagct ccctcagatg tgacaagaag ggcaccatca
    14761 ccagccatag agataataac attttgagca cccttagcca gtaactcacg agcgtatttc
    14821 tcaatttcat ctaaactttc gagtttaacc ccaaaaatcg ctccaagttc atgattgttt
    14881 ggttttacaa gaagaggctg gtagtccaaa ctatcaatta aggtctgtcc ttcaaagtca
    14941 cagaccactt gcgcaccagt ctggcgcgtc aaggaaatca aatccttata gataacattg
    15001 cctagatttt tagcacttga acctgcaaag acaactgtat cttctgctgt cagactagat
    15061 aaaatagctt tcaattcttc tagctgaacc gattcaacag ttggacccgt tccgttgatt
    15121 tctgtttctt ggtctgcttt gattttaaca ttgatacgag tatcttctgc cacctggaca
    15181 aaacgtgtct cgatttcttc ctctgctaaa gtatctgtga taaatttacc agtaaagcca
    15241 ccgataaatc ccgtcgctgt atttggtata ttcaaacgtt tcaagacacg gctgacattg
    15301 attcctttcc caccagcaaa cttatcatca ctgtccatac gatttacact accaactttg
    15361 acttggtcca aacgaacgat atagtcaatg gatggattga gtgtgactgt ataaatcata
    15421 cttctattac ctccgttttc tccttaataa cctgcaagag ctcatgccct tgactagtga
    15481 taacgatagc gcgtttgagt ggtgctacct tggcaaagca agtttgtcca atttttgacg
    15541 aatccaccaa gacgtaggtc tgcttggcat tctccaaaat agctcttttc acagctccct
    15601 cctccatatc aggagtcgta taatagccat cgtcaacacc attcattccg ataaaggcac
    15661 ggtcaaagtg caattggtta atctggttaa gagcaacgcc cccgatacta gcatctgtcg
    15721 ccgtcttgac gtttcctcca accatgacag ttggaatctg cttttcaacc aactgagcgg
    15781 catggtgaat ggagttggtc acaactgtaa cattcttatt gaccaattca tgaatcaaaa
    15841 aagcagttgt tgttccagca tcgataaaga tgacatcttt ttctttaatg agagaggctg
    15901 ctttctgagc cagcaacttc ttttcttgaa ggtttttgac agatttttct tgaatggttt
    15961 cttcttcctg taaggagtgg gggagttctg ctccaccatg cacacgacga agcttgtttt
    16021 ccgcttccaa ctcatccaag tctcttcgaa ccgttgattc tgacgtttct agcaaactaa
    16081 ctaatttttc tagagaaact acatgatgct gatttaactc ctctaaaatt agttgtttac
    16141 gttctgtttt taacactgaa tcacctcctg ttatcgttta cactattcat tctatcatac
    16201 tttctttcga agtcaagcat tttttatcat tttctttcaa aatctatcat ttttcttatt
    16261 tctagaattt ttattgcaag ataagagcct ttatggtaga ctatttgagt aagtattgga
    16321 agattactca agaggcttaa gaggccgtgt tggaaacgcg gtaggcgtgt aatagcgtgc
    16381 gtgggttcga atcccatgtc ttccgtcgaa aaaagattat ttttaggatg cgacaaatgt
    16441 tgtatccttt tttctatcct tttttctatc ctttttttaa aatagggtct gtttgtgact
    16501 ccctttttag caaaagaatg caaaggaata ccaaagataa aaataaaaaa cgttgattta
    16561 acaacgtttt accaaggaat gcaaaagaat gcaaaggaat aatggagccg gtgggagtcg
    16621 aacccacgtc caaacacctg ccaacatatt tgtctacaac cataggttat gtattatttt
    16681 aacagcccct cgacacataa ctcaagtcta gaaactgcga gtctatcaat ctcttatcaa
    16741 gccgctagac aaggcttaat cgtatctcgc taataataag acctgtcatt gaacacgagc
    16801 aatccaaatc gggtcacgcc tgctggtttt taggcagcta gagcgtaaga agtgttattt
    16861 tttgcagtta tatttaactg agcgtttacg tcgccacacg agtcgcaaaa tatgcctcat
    16921 aatgcctgtc gaatccgtaa cgaccccaaa agacaataaa accattatac cttatctaac
    16981 tcaaaaatgc aaaaagaaat cacataccta agatgaagct atctgttttg aagaaattca
    17041 cgaagagaat tattctaaaa tactttcaga tgagaatttt cgtactctct ttatttcaat
    17101 tcatttgagt ttaaaaacta taaaatcaat ccttatatta ttcggt
//
LOCUS       DAVLTM010000035        16784 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLTM010000035 DAVLTM010000000
VERSION     DAVLTM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.035"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(111..1745)
                     /locus_tag="VYN16_000990"
     CDS             complement(111..1745)
                     /locus_tag="VYN16_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403537.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1726..2475)
                     /locus_tag="VYN16_000991"
     CDS             complement(1726..2475)
                     /locus_tag="VYN16_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403538.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2654..4108
                     /locus_tag="VYN16_000992"
     CDS             2654..4108
                     /locus_tag="VYN16_000992"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1403539.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VYN16_000993"
     CDS             complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VYN16_000993"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1403540.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(6838..7323)
                     /locus_tag="VYN16_000994"
     CDS             complement(6838..7323)
                     /locus_tag="VYN16_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1403541.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7598..7837)
                     /locus_tag="VYN16_000995"
     CDS             complement(7598..7837)
                     /locus_tag="VYN16_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403542.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILSHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8082..9152)
                     /locus_tag="VYN16_000996"
     CDS             complement(8082..9152)
                     /locus_tag="VYN16_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1403543.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9149..9922)
                     /locus_tag="VYN16_000997"
     CDS             complement(9149..9922)
                     /locus_tag="VYN16_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403544.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9919..10725)
                     /locus_tag="VYN16_000998"
     CDS             complement(9919..10725)
                     /locus_tag="VYN16_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403545.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10706..11863)
                     /locus_tag="VYN16_000999"
     CDS             complement(10706..11863)
                     /locus_tag="VYN16_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403546.1"
                     /translation="MKKSIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRNIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12024..12929)
                     /gene="murB"
                     /locus_tag="VYN16_001000"
     CDS             complement(12024..12929)
                     /gene="murB"
                     /locus_tag="VYN16_001000"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1403547.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13041..13793)
                     /locus_tag="VYN16_001001"
     CDS             complement(13041..13793)
                     /locus_tag="VYN16_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1403548.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13803..14534)
                     /gene="budA"
                     /locus_tag="VYN16_001002"
     CDS             complement(13803..14534)
                     /gene="budA"
                     /locus_tag="VYN16_001002"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1403549.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIGEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14676..15947
                     /locus_tag="VYN16_001003"
     CDS             14676..15947
                     /locus_tag="VYN16_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1403550.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15967..16782)
                     /locus_tag="VYN16_001004"
     CDS             complement(15967..16782)
                     /locus_tag="VYN16_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1403551.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
BASE COUNT         5021 a         3803 c         3316 g         4644 t
ORIGIN      
        1 acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca ttctaacaaa
       61 ctataaaagc gtttgtcgaa ttgaatttat caagcaagcg accaaccagc tcatcttttt
      121 tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc aagagcaagc
      181 aggaggataa gagctcctac tcccaagctg atggcaagga tagtggagag agactgaacc
      241 aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa caataaaact
      301 atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca attggttgac
      361 agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc aaggatccat
      421 gccacaataa ggtaaatcat cgaaatgatg ggcaagccta gatatagaga aagaccaagc
      481 aaagtcagaa ctggtaaaaa ggactggaca gcatatataa tccaaaattt cactttcaca
      541 taacgagcaa agtcaaaggg taaactctta agaaaatcaa cattttccct ctccaaggac
      601 aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa cactcctaca
      661 aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga atctcggatt
      721 ttggaaatca gaccgatcat catgagataa ggaaagaaag cacttgtaaa aagcactgta
      781 accacgccag taccctgtcc caagagggtg aggtggtagc gtaaaaccat gcggaaaaat
      841 ccctttttag tggttgaaat tctctccttg ctgcgacgtt cttttttgac cttctcctca
      901 ctattaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt cagataaagc
      961 aggaagagag ttagtcctat ccaagcgagc agacccaata tggcttctgt cgaaaaaggc
     1021 tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat gtctctaact
     1081 ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa aggtatgaga
     1141 actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa gaccgtagtt
     1201 tgagccaaga aatgtactgc cactaccatc actaaagtaa cagagacaaa taataaggtc
     1261 aaggacagta gcatcaaagg caaacccaac cacagagaag gtgctagcct aatgtagagg
     1321 accagaaaat aagctaggat tggtacaatt ccagctagag ctggcaagag gacagacaga
     1381 cctttggcca ttataatctc tgattcttta aaggcatagg gcctatagga ggccaggtcc
     1441 ttactctcat aaaagacatt gtaaaatgca gtgaaagaag ttgaaaaggc cagcaccagt
     1501 aaaatagcaa ccatcgagct aaaataaata ggtatttcct caaaaggaaa atgaatggct
     1561 atattactaa aacagatgag catcaagaga ctggaaaaaa tgtaagaact taggactcta
     1621 gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag attagccaga
     1681 ttagcttctt gagatgaata gaggatattg atatcaacta attttttaat gaccttgaga
     1741 cgcatctgaa acctcctctt ttctaccagc aaggctaagg tagatacttt ccaaagactg
     1801 gtctgggttg tcttttctca agtcctctac cttaccacaa taaatcaaat gccccttttt
     1861 caaaatagca atccgatcac agacttgctc agctacctct agcacatgag ttgaaaacaa
     1921 aactgtctta cctttttgcg catgctcctt catcatttgc ttcaaatcaa aggcggcctg
     1981 gggatccaaa ccagtcaagg gttcgtccaa aacccaaata tcaggatcag acaagagtgc
     2041 cccgatgaca aagactttct gacgcattcc atgagaaaga gtttcaataa cctgatagcg
     2101 attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca agtcagatct
     2161 actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta agcgtaaaaa
     2221 taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc gattttccga
     2281 taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac tgactaggga
     2341 ttttatagtg gtcgattttc cagccccatt atgaccaatc aagcccataa tctctccatt
     2401 ttcaatctgc aaattgagat tactcaaggc ctttttatca ccatacaact tactaacatt
     2461 ttgaaattca atcatgggac aactcctatc tttatctgta ttatatatcg ctatactagc
     2521 actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt ccttctatct
     2581 tacgcaaacg tttgcgctaa tagatgcttt atgatagaat aaagaacaag attgacaagt
     2641 aagaggaaac attatgcaaa atcaaacact catgcaatac tttgaatggt atctgcccca
     2701 cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc atctggggat
     2761 cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg tcggctatgg
     2821 ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc gcaccaagta
     2881 tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcgcagg gaattcaacc
     2941 tatggccgat gtggttctca accacaaggc tgctgctgat cacagggaag cctttcaagt
     3001 tattgaagtg gatcctgtag accgtacagt tgaacttgga gaacccttca ccatcaatgg
     3061 ctggactagt tttaccttcg atggtcgcca agatacctac aatgacttcc actggcactg
     3121 gtaccacttc acaggtacag actatgatgc caaacgccgt aagtctggga tttatctgat
     3181 ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta
     3241 cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta
     3301 tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt
     3361 taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg aaaaatacgg
     3421 tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca atctggacta
     3481 tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccatc agaatctctt
     3541 tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag atagcctggt
     3601 tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc aacgaggaca
     3661 agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt
     3721 acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt caggccagta
     3781 tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa aagatttggc
     3841 ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg
     3901 tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa acagcaagtc
     3961 aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca
     4021 aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt
     4081 aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg
     4141 atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt tacttgatta
     4201 caatttacct gctactgcat ccaacaattc ttggatctta ggttggttgc ttcctcctgc
     4261 catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac
     4321 aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc
     4381 gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa
     4441 ggtacgaagg gcacctgcat cggatacaga cacttgacta gcaatgtaac gatgaccgtt
     4501 gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa
     4561 ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg
     4621 tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag catcctcttg
     4681 ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc gagttcctga
     4741 accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacatg
     4801 agtaccacca caaagttcaa tagagtagtc accaatagtc acgacacgaa cttccttgcc
     4861 gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa tatccgtttc
     4921 aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt caatcgcacg
     4981 caattcttca gcagttacag cttggaagtg ggtaaagtca aagcgaagga attcaacttc
     5041 gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag
     5101 caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa
     5161 ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc
     5221 atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat
     5281 ttgtccgtgg tcagcgacct gtccacccat ttcagcatag aatggcgttt ccgcaaagat
     5341 aagtgaggta gttccttctg atacagcttc tacttctgca ttgtcagcaa cgatagccac
     5401 caatttagaa gacaattggc tagcattgtg gttgaagaca ctttctacag tgatgttttg
     5461 aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac gcgcgcgttc
     5521 ttgttgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac cagcctcttc
     5581 agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt taaagacatc
     5641 ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc cttgggcaaa
     5701 gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa cgattttctc
     5761 aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt ttccaacagt
     5821 tggaacgagt ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc
     5881 acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa gggcaccatc
     5941 accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc tcatgttgtc
     6001 gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa tcggcatgaa
     6061 gaggtccgtt tcaaagttgg tcttagcccc ttggataacg gccaccaaac gctccaaacc
     6121 agcgcccgta tcaatgttct tatgtggtaa ttccttgtat tcgctacgag gaacagcagg
     6181 gtctgcgtta aattgtgaca aaacgatgtt ccagatttcg atgtaacggt cgttttcaat
     6241 atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc cacggtcaaa
     6301 gaaaatctct gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt tgtcctcaat
     6361 tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc
     6421 atctggatag taagtcatgt aaagtttctc agctgggaag tcaaaccatt cagggcttgt
     6481 caaaagctca taagcccaag tgatggcttc atcacggaag taatccccga tagagaagtt
     6541 ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt cgatgtcgtt
     6601 ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa tggtcccgtc
     6661 aaagtatttc ttgagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac
     6721 aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga aatcaagcca
     6781 catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt tacttgttta
     6841 atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag
     6901 gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc
     6961 cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat tcaaatccca
     7021 gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc gccagaaggt
     7081 caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag
     7141 caaggtcaag atttctttac taatctcact gacttgttca ccagccgcat catagatggt
     7201 aaaggtttta ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc
     7261 atccttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt
     7321 catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt
     7381 accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagta
     7441 gcatgacttc ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg
     7501 aaaatcaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca actggaaatg
     7561 atctccgttg aattagacca attccctaca tctctgatta ctttttcagg atatattttt
     7621 tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa
     7681 tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc
     7741 gatccaagcc atcctttaaa tgggatagta taacagtttg gttaacaatc ataaaggttg
     7801 gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag
     7861 gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag
     7921 tacggcaagg caacgctgac gtggtttgaa gaatataggc ttagtatact actaggcaag
     7981 caaataagca actagaatag aaaaagatag ggttctaaaa actgacttct attccttaaa
     8041 aacgaaccag cttgactgat tcgtcttctt acgtttatct cctacttccg atacatttta
     8101 aactgtagga agaggtcgct atatttccct gtccatttat ggtcaaattt ctcataaact
     8161 tctaggtgtt tcatggtttc aacatctgga tagaaggcct tatcttcctt tgtttcctct
     8221 gggagcaatt ccttcgctgg taggtttggt gttgaatagc cgacatactc cgcattttgg
     8281 agagcgtttt caggtttcaa cataaagttg ataaaggcat aggctgagtc ttggttttta
     8341 actgttttgg gaatgaccat attgtcaaac caaagattgc tggcctctgt cggtaccaca
     8401 taacgtagat tttcattttt ttctaacatt tggctggctt caccagagaa ggtcacgccg
     8461 attgcaacat tattctgaat catgtagccc ttcatctcgt ccgcaacgat agccttgata
     8521 tttggagtca gtttgtagag cttatccact gtctcttcca actgctgcgg atccttggag
     8581 ttgaggctgt agccgaggga attgagtcct agtcccagca cctcacgcgc cccatcaaaa
     8641 agcatgatag aattcttata ctccggcttc caaaggtcat cccaatgctc aggcgcttca
     8701 tctaccatgg tttcgttgta gacaattcct aaggttcccc agaagtaagg gatggagaat
     8761 ttattacctg ggtcaaagga ctggttgaga aactctggtc cgatattttc gattccttca
     8821 atttttgaat aatcaagcgg aaccaagagg tcttcgtcct tcatcttgtt aatcatgtat
     8881 tcacttggaa tggcaatatc gtaggtcgtt ccaccctgct ttatcttagt gtacatggct
     8941 tcgttggagt caaaagtctc gtactgaact tgaattcctg tttcttctgt aaactgagtc
     9001 aagagttcag gatcgatata gtctccccag ttatagataa ccaatttttg actatctcga
     9061 ctattgattt tactatctaa atgagtcgca attccccaca agacaaggat aatcgctgca
     9121 attcctgcta aaaatgaata gatttttttc atgcttgctc ctccttctca cgagagataa
     9181 agtaataacc tacaactagg ataatactaa agagaaagac tagagcagac agggcattga
     9241 tttctaagga aatccccttg cgagcacgag agtaaatctc gactgatagg gttgaaaagc
     9301 catttcctgt tacaaagaag gtcacggcaa agtcatctaa cgaataggtg aaggccatga
     9361 aataaccagt aatgatagac ggagtcaggt aaggaagcat gatttccttg aacatctgaa
     9421 attgactagc tcccaagtca taggccgcat gaatcatgtc gccattcatt tccttgagtc
     9481 gaggcaagac catcaagacc acgataggaa tggagaaggc cacgtgacta gatagaacgg
     9541 tcaaaaagcc aagtgaaaac ttgagttggg taaagagaat caagaagcta gcaccaatca
     9601 taacgtcagg cgcaaccatg aggatattat tgagtgatag aaaggcttct tggtatttct
     9661 tacgagactg gtagatgtaa atggcaccaa aagtcccgat aatggtcgct atcaaggctg
     9721 ataggaaggc caagaaaaat gtctgagcca aaatcagcat gagtctccca tctccaaaca
     9781 tggtttcaaa gtgagtccag ctaaaacctg taaagctatt catatcatca ccagcattaa
     9841 aggcatagcc aatcaagtaa aagataggca ggtagaggac cagaaagacc agtcccagat
     9901 aaaggttggc aaattttttc atcgttctct cctttcctta gtcacccaca tggtgatgaa
     9961 catggtcagg atgagaatca caccgatagt tgaacccata ccatagttgt cattggttag
    10021 aaaattctgc tcaatagccg tccccaaggt gataacgcgg ttcccaccaa tcaaacgggt
    10081 cagcatgaag agactcaaac ttgggataaa gaccgactga accccacttc gcacaccgtt
    10141 catagataga gggaagatga catgacggaa ggtctcccac ttagttgcac caaggtcgta
    10201 actagcattg atgagattat tatccatatc gtctaagaca ttgaaaatcg gcaaaatcat
    10261 aaaggggagc tcgatgtagc ttgcgacaaa gataaatgag aaatcagtaa atagcaactg
    10321 ctgtgaacca attccgataa attccaagaa ttggttaata gagccattct gaccaaaaat
    10381 cccgataaag gcataagcct taagaagaag gttgatccaa gttggcagaa taatcagcat
    10441 aatccagagt tgacggtgtt tgagacgggt caaaaagagg gccgtcggat aactgataag
    10501 cagtgtcaca aaggtcacaa tgcctgcata aagcactgag ttgaaactca ttttaagata
    10561 ggtcaagttt tgtgacgcaa agtaagattt gtaattttct aaactgaact ggccttcgat
    10621 gttgaaaaag gattgaccga aaatcaagac caagggtgcc aatacaaaga gggcaatcca
    10681 aagcatgtag ggtactacaa agagtttaga gcttgttttc ttcatctctt tcctcctcga
    10741 ttgcattgat caaacctgct tcttgctctt cgatttctac gtactcctca atacgagcat
    10801 cgaactcttc ttcggtttca ttgagacgca tgatgtggat gtcttctggt tcaaagtcca
    10861 gaccgatttc ctcacccacg atggccttac gggttgagtg gatcatccat tcatttccaa
    10921 gttcgtcata ggcgataatt tcataatgca ccccacggaa gagctgggta tcgaccttaa
    10981 cttgaagctt gccttcttca ggaagggtaa tgcgcaagtc ctctggacga ataacgacct
    11041 caacaggttc atttggcttc atcccaccat caaccgcttc aaagcgtttg ccgttaaatt
    11101 cgaccaagta gtcctcaatc atggtacctg gcaagatgtt tgactccccg ataaaggtgg
    11161 caacaaagtg gttgattggc tcatcgtaga tgtccacagg ggttccagac tggacaatct
    11221 cgccatcatt cataacgaaa atccagtcac tcatggcaag agcttcttcc tgatcgtgag
    11281 tgacaaagac aaaggtaatg cccaatcgtt gttgcagttc acgcagttcg tactgcatgt
    11341 ctgttcttaa tttcaagtcc agcgctgaca agggctcatc caacaaaacc acacggggtt
    11401 ggttgatgat agcacgggcg atagccacac gctgacgttg tcctccagaa agtttgcgga
    11461 tggaacgttt ttcataacct tccaactgaa ccatcttgag aacttccgct acacgttgct
    11521 cgatttcttt cttatcaatg ttacgcaagc gaagtggaaa ggcaacattt tcaaacacat
    11581 tcatatgtgg gaacaaggca taggattgga agacagtatg tacgtcgcgc ttgttggttg
    11641 gaatatcatt gatacgaaca ccgtctagca tgatatctcc tgtcgtcgca tccagtaaac
    11701 ctgcaataat gtttaggata gttgatttcc ccgaaccaga tgcacctaga agggtgtaga
    11761 atttcccttc ttccaactca aagttgatgt ctttgagaac cttggtgttg ctgtcttcaa
    11821 aaactttaga gacgtttttg aattcgataa ttgatttttt caattggcat aaattccttc
    11881 tttttcatag attaaccgat cggggctctg tcaggtcgcc actacctctt gcagggagta
    11941 aaaccacctg catacatctt cgctaccaat aggctttcac ccccctttcc atgacatagc
    12001 agcagctttt caaatacagc ttcctacttg ctttcaccca agatccggac ttctctttca
    12061 agcgtaatac ctgagtgttc cttgactttt tcgataaccg attggatcaa gtcctcgtag
    12121 tctttggccg ttccatctgc gacattgatc ataaatcctg catgcttttc tgacacttct
    12181 acgccaccga tacgatagcc tttcaagcca gcttctgaaa ttaactgacc tgcaaaatgc
    12241 ccgactggac gcttaaagac cgagccacaa gatgggtatt ccaaaggttg cttgagttca
    12301 cgtaggtgcg tcaagcggtc catttcctgc ttgataacct gatgggttcc tggggctagg
    12361 gcaaatttaa ctgacaagac aactgcacca gactcctgaa tagctgaatg gcggtaacca
    12421 aaagccaagt ctttagcaga cagggtttcg atttctccat ccttggtcaa gaccttacaa
    12481 gactgcaaga tgtgagcaat ctcgccacca taggcacccg cattcataaa gacagcaccg
    12541 ccaacgcttc ctggaatacc acaagcaaac tcaaagccag tcaaactatg acggagggca
    12601 atgcgagttg tttcaatcaa gttagcccca gcttctgctt caatggtata gccatcaaca
    12661 gaaacgttat tgagcttgtc acacaagatg acaaatccac gaatcccacc atcacgaacg
    12721 atgatattgc ttgcattgcc aagaaccatc caagggatat tttcttggtt ggcaaatttc
    12781 acaacgcgag ccaactcaaa acgatttcgt ggaaagacca aataatcagc ctctccacct
    12841 acttttgtat aactatagct atgcaagggt tccttaaaac ggatatcaat tccttctaag
    12901 atttcaagca ttttttctct tacagacatg tcactcttcc ttttacaaaa ttcattccat
    12961 tataccattt ttagagacat ttgacgacca taaaaatacc ttgtttggat tttgcataag
    13021 aaaaagaggt tccccccttt ttatgatttt ttacaaaaga tttccttggt tccataggca
    13081 accagaacga gctccagtgc tagaatcact tcaaccaaga ctggatttgt caaccagcct
    13141 acttggaaaa gagatggtgc cagatcaaag aaggcatgca agccataggc tgctaggaga
    13201 taaatccatt tcttctggcg aacagcttgg taaacccaaa ctgtcaaaag taattggaaa
    13261 ccaagcgcca agattcgctc aaaaccaagc aaataaatct gccagaccga aagtgactga
    13321 atggttttta acatattttc agacagtaat tgcataacct gtggattctg agtttgaact
    13381 gccgaaagaa cgatgtaaag attgagtaaa ctaataaggc ctagaaaaat caactccaag
    13441 ccaccatgcc ctaggccata ggccaaggca tctgcctttt ccaaacttct ctttttctcc
    13501 aaccatttga agaaaacaag acgagccgtt tcctcaaaaa atgctgccat ggctaggcca
    13561 tagataatat agataagcgg atgatcttgc aagagggcaa tactaccgtc tttttgagga
    13621 tgcaaaatca agatatgcac cagtttttcc aaaatctgcg aagagacaaa gaaagcgact
    13681 gcacccaagc ccaagacagc tagattaatc tggtatttct ttttggcaaa ccaaatgctt
    13741 cctatcagaa aagctagcaa cagcaacatg gtaataatga tatgaatggt cattttcttc
    13801 tcctattctg ccttttcaat atcttttttc atctcgtcaa cattgaactt agcaaacaag
    13861 tattgacggt cttggactgg gaaacgttgg tccaactggt caactgctcc cacctcgata
    13921 atgccttcct tgatgacaaa atccatgaca tgtccaccga aggtcaaatc atctgatatg
    13981 aagtgcagat ggtagcctgc cacactgacc ccatggaaaa tctcaggcgt ccagaaacca
    14041 acaatagtcc ccgcaacatt gtcacgacta tattccggtt gatgggttgc gacatcagca
    14101 aacttggtat cgggtgttga cttaggaatc atacgcacat gcatatgtga aaattccccc
    14161 cgaatcttga tagagcggaa aagattttcc ccatcataat aagactcaat tcgttctttc
    14221 aattccttgt ctgtcatctc aaagcgctgg cggaaaatga cctctgcctg atgcggtacc
    14281 actgcagcgt aaggaataag ggcatctggt gacacttccc caatttctgg ctggtctcct
    14341 gaccctttgg cctgataagc cttgccatcc aagacaatca attccccatc aatggaatcc
    14401 aaggttccca aaccaaggtc accatgttct agcaattctc ctactgtcat agtaccacca
    14461 taaaggccag ccatcaaggc tccaagggta ttgtattgaa ataatttcac tggttcctgc
    14521 acgttcttat ccattcactt tcttgtctgt tattctataa atcttactcc taagtatacc
    14581 acatttgccc ctagatgtga acgagagaaa cgctctagac attgccaaga aggaaaaaaa
    14641 aaggtacaat gtaacaaaat caagggaggt ctggaatgaa gaaacaaagc aagtacaaag
    14701 aggtcgtttc ctatctgaaa aatggtatcg agtctggacg atttccgacg ggtagtcgcc
    14761 tgccttctat ccgtcaactg agccttgact ttcactgcag caaggacacc attcaacgag
    14821 ccctgctgga attacggcac gaacaatacc tctatgccaa gcctcagagt ggctactatg
    14881 tattagaaca agggcaacat caagacctag aaatcgaggt taccgacgaa catgccagtg
    14941 cctatgacga tttccgactc tgtgtcaatg aaaccttgat tggccgagaa aactacctct
    15001 tcaactacta tgacaatcaa gaaggattag aagacctaag acagtccatt cacaaactcc
    15061 tctttgagca agctctctac tgcaaggcta accaactagt actgacttct ggaacccaac
    15121 aagccttatt tatcctctct caaatatcct ttcctagaca agccaaggaa atcttggtgg
    15181 aacagccaac ctaccatcgg atgaatcgcc tcttgattgc acaggggctg gactatcaaa
    15241 cgattgaacg aggcattgat gggattgact tggaggagct ggaaggccac ttcaaaacag
    15301 gaaaaattaa atttttctac accattccta gatttcacta tcctctggga cattcctatt
    15361 ctgagcaaga caaacgatct attcttaact tagctgccaa gtatgatgtc tatatcgtag
    15421 aggacgatta tctgggtgat ttggactcca agaagggcca aaccttccac tatcttgata
    15481 cagaggagca tgtcatttat atcaagtcct tctcgaccag cctttttcct gcccttcgta
    15541 ttacagcact cattcttcca aatgctatca aagaagcatt tgtggcctac aaaaatatcc
    15601 tagactacga cagcaacctc attatgcaaa aggccctgtc actctatatt gacagtcaat
    15661 tgtttgaaaa aaatcgtttg gctcgcttga ccaatcatga atcttaccaa aaacaaatcg
    15721 aggaaaggat aactaaaaca ccttgtcccc ttcctcatta tcccctacac gatggtttat
    15781 tgctagacct gagacagtat cctaaaatcg ccagtctcaa acacagtcaa ccgggcttgg
    15841 acttctttga agaggcctat ttaagtacct gtccttatca atttgccaag gtgtccctag
    15901 acaatctgga aaatgtttta aactatttaa aagcagaatt ggaatgacat ccaactctgc
    15961 tttttcttat tcttcaactt cagttagact tgctgctttt tcaagatagc tttggtaaat
    16021 tccttcatcc ttaagttttt ggatgacctt gtctaccact tctttcaaat catcactatt
    16081 ttttctaaga gcaacggcat tggcgtcgcc gtccttcatc ttcaagctga cagttgcgac
    16141 agctaagcca gcgtttttag cagcataact aagtgcaaca ggctcatcca tatgaacagc
    16201 atctactttt ccagcctgca attcattgac tgcttcaccc atattagtta gggaagttaa
    16261 ttgagctttt ggcaattgtt ccttgaccat tgattctgga acagtccctt tttgggctgc
    16321 aatattagca ctttctaggc tagttaaatc cttgtatttt tccacatcag ccttacgaac
    16381 caagaaacta atcttgtttt catagtatgg gattgaaaaa tcaaagactt cttttctctc
    16441 gtcagtagca ctaattcctg caactgctag gtcagcctta ccagtttgaa gactggtcaa
    16501 aacattgtca aaactcatgc ttgagatttc caacttaacc ccaagttcat cagcgatagc
    16561 ctgagccatg tcgatgtctg caccgactac ctggttcttt ccatcaacca atgattgaaa
    16621 ttcaaagggt gcatagtcag gactggttgc cacaactaat ttcccctttt gcttaatggc
    16681 ctcaacagct gactgagaac cattagaacc tgactggcaa gctactaaga agaaacttgc
    16741 aagaaaatta cataaaacaa atatccattt ttttgatttc ataa
//
LOCUS       DAVLTM010000036        16675 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLTM010000036 DAVLTM010000000
VERSION     DAVLTM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.036"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            230..901
                     /locus_tag="VYN16_001005"
     CDS             230..901
                     /locus_tag="VYN16_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403552.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLLESGVLLSAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISPPS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(953..1150)
                     /locus_tag="VYN16_001006"
     CDS             complement(953..1150)
                     /locus_tag="VYN16_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403553.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1143..2051
                     /locus_tag="VYN16_001007"
     CDS             1143..2051
                     /locus_tag="VYN16_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1403554.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2048..2509
                     /locus_tag="VYN16_001008"
     CDS             2048..2509
                     /locus_tag="VYN16_001008"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1403555.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2499..3386
                     /locus_tag="VYN16_001009"
     CDS             2499..3386
                     /locus_tag="VYN16_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1403556.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3389..5284
                     /gene="cbpE"
                     /locus_tag="VYN16_001010"
                     /gene_synonym="pce"
     CDS             3389..5284
                     /gene="cbpE"
                     /locus_tag="VYN16_001010"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1403557.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHLDTKNSNTKNFIKNLSPEIIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQHINAASKDYDATVFDIRQDGLINISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     RWFYLTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQY
                     KGQWYYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWH
                     KKANDWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVS
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            5364..6494
                     /locus_tag="VYN16_001011"
     CDS             5364..6494
                     /locus_tag="VYN16_001011"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1403558.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6504..7766
                     /locus_tag="VYN16_001012"
     CDS             6504..7766
                     /locus_tag="VYN16_001012"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1403559.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSHESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            7770..8567
                     /gene="proC"
                     /locus_tag="VYN16_001013"
     CDS             7770..8567
                     /gene="proC"
                     /locus_tag="VYN16_001013"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1403560.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLILEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8787..9425
                     /locus_tag="VYN16_001014"
     CDS             8787..9425
                     /locus_tag="VYN16_001014"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1403561.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEVKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9422..10312
                     /locus_tag="VYN16_001015"
     CDS             9422..10312
                     /locus_tag="VYN16_001015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1403562.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10352..10669
                     /gene="yabA"
                     /locus_tag="VYN16_001016"
     CDS             10352..10669
                     /gene="yabA"
                     /locus_tag="VYN16_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1403563.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10672..11550
                     /gene="rsmI"
                     /locus_tag="VYN16_001017"
     CDS             10672..11550
                     /gene="rsmI"
                     /locus_tag="VYN16_001017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1403564.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASKGVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEENQEND"
     gene            11685..13019
                     /gene="trmFO"
                     /locus_tag="VYN16_001018"
     CDS             11685..13019
                     /gene="trmFO"
                     /locus_tag="VYN16_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1403565.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            13103..13840
                     /locus_tag="VYN16_001019"
     CDS             13103..13840
                     /locus_tag="VYN16_001019"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1403566.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            13849..14406
                     /gene="frr"
                     /locus_tag="VYN16_001020"
     CDS             13849..14406
                     /gene="frr"
                     /locus_tag="VYN16_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1403567.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            14466..15320
                     /gene="cvfB"
                     /locus_tag="VYN16_001021"
     CDS             14466..15320
                     /gene="cvfB"
                     /locus_tag="VYN16_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1403568.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            15329..15544
                     /locus_tag="VYN16_001022"
     CDS             15329..15544
                     /locus_tag="VYN16_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1403569.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            15630..16634
                     /locus_tag="VYN16_001023"
     CDS             15630..16634
                     /locus_tag="VYN16_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1403570.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIYQIDFVHFSAKDVVRHPVVAQIIRAYEPAPVKKEESEELESQPLSEG
                     "
BASE COUNT         4897 a         3048 c         3945 g         4785 t
ORIGIN      
        1 agctgataca aggtggagca gatccctatg gtaaagaacc taatggttat tatccttggc
       61 aaatggaacc agtattaact atgccggttc atggattttg tcccagagga accgatgatt
      121 tagtatatat tgtaggaggt aatcatcttt gcaaaggaag tgctgcgaga ttttaagtaa
      181 aatttattag gaatatgaag aaacaagggg agaaaacaga ggatttaata tgaaaaaacg
      241 agctattcaa attttactag cattgtcctt aattttttac aaatcaactt ggttttggag
      301 gcttttcaat tatctcgcaa agccctatct accagcaagt cgtgaatttt ttcagattct
      361 acttttgctg gaaagtggtg ttcttttgtc agcagtcatc tatctactag tttttgcagg
      421 aaagaagatt tttcatttca agtggcagtt gaggtacttt atctaccttt tactgggcta
      481 catcatttcg tatatgtctg actttctctt ttcgtatttc atatccccgc cttcaaatca
      541 aatttctttg aatgaaacga tagaaatgat gggacgacag gagttccctt atttcttgct
      601 catcgtttgc ttcatcgccc ctattgctga ggaattgatt tatcgcggtg tgattatgac
      661 aacctttttc aaaaactcgc cttggtacgg agatgttttg ctttctgcta ttattttcgg
      721 ttatattcat atcaattttg ctttaactcc tcttgctttt ttcatttatg ctagtggagg
      781 tcttatttta gctctattgt atcgcatgac taaaaatctc tactatccaa tactagttca
      841 tattctcatc aatatcactg ccttctggga tgtgtggtta ctcctatttt caggaagtta
      901 gcttactaaa aaaatgtcgg aattttccgg cattttcttt tttcacaaat agtcaacgtt
      961 tttctttccg atactgaagt ggtgtgtagc cacttatttt tttgaattga ttttgaaaat
     1021 aagattggcg tgagaaaggc agatagtgaa gatagttaag aagaatagga tgttcttttt
     1081 tcctttttgg aaaacttcta aaatatggta taatgaaaag ataaagaagt tgggggtaga
     1141 agatgaacat tcaacaatta cgctatgttg tggctattgc caatagtggt acttttcgtg
     1201 aagctgctga aaagatgtat gttagtcagc cgagtctgtc tatttctgtt cgtgatttgg
     1261 aaaaagagtt gggctttaag atttttcgtc ggaccagctc agggactttc ttgacccgtc
     1321 gtgggatgga attttatgaa aaagcgcaag aattggttaa aggatttgat atttttcaaa
     1381 atcagtatgc caatcctgaa gaagaaaaag atgaattttc cgttgctagc cagcactatg
     1441 acttcttacc accaactatt acggcctttt cagagcgcta tcctgactat aagaacttcc
     1501 gtatttttga atcaactact gttcaaatat tagatgaagt ggcgcaaggg catagtgaga
     1561 ttgggattat ctacctcaac aatcaaaata aaaaggggat tatgcaacgg gttgaaaagt
     1621 taggtctgga ggtcatcgaa ttgattcctt tccataccca tatttatctc tgtgagggtc
     1681 atcctttagc ccagaaagag gaattagtca tggaggattt agcggattta ccaacggttc
     1741 gtttcactca agagaaagac gagtaccttt attattcaga gaactttgtt gataccagcg
     1801 ctagctcaca gatgtttaat gtgacagacc gtgccacctt gaatggtatt ttggagcgga
     1861 cggacgccta tgcgacaggt tctggatttt tagatagtga cagtgttaat ggcattacag
     1921 ttattcgtct caaggataac ctagataacc gcatggtcta tgttaaacgt gaagaagtgg
     1981 agcttagtca agctgggact ctcttcgtag aagtcatgca agaatatttt gatcaaaaga
     2041 ggaaatcatg aaaaaaagag caatagtggc agtcattgta ctgcttttaa ttgggctgga
     2101 tcagttggtc aaatcctata tcgtccagca gattccactg ggtgaagtgc gctcctggat
     2161 tcccaatttc gttagcttga cctacctgca aaatcgaggt gcagcctttt ctatcttaca
     2221 agatcagcag ctgttattcg ctgtcattac tctggttgtc gtgataggtg ccatttggta
     2281 tttacataaa cacatggagg actcattctg gatggtcttg ggtttgactc taataatcgc
     2341 gggtggtctt ggaaacttta ttgacagggt cagtcagggc tttgttgtgg atatgttcca
     2401 ccttgacttt atcaactttg caattttcaa tgtggcagat agctatctga cggttggagt
     2461 gattatttta ttgattgcaa tgctaaaaga ggaaataaat ggaaattaaa attgaaactg
     2521 gtggtctgcg tttggataag gctttgtcag atttgtcaga attatcacgt agtctcgcga
     2581 atgaacaaat taaatcaggc caggtcttgg tcaatggtca agtcaagaaa gctaaataca
     2641 cagtccaaga gggtgatgtc gtcacttacc atgtgccaga accagaggta ttagagtatg
     2701 tggctgagga tcttccgcta gaaatagtct accaagatga ggatgtggct gtcgttaaca
     2761 aacctcaggg aatggttgtg cacccgagtg ctggtcatac cagtggaacc ctagtcaatg
     2821 ccctcatgta tcatattaag gacttgtcgg gtatcaatgg ggttctgcgt ccagggattg
     2881 ttcaccgtat tgataaggat acgtcaggtc ttctcatgat tgctaaaaac gatgatgcgc
     2941 atctagcact tgcccaagaa ctcaaggata aaaagtctct ccgcaaatat tgggcgattg
     3001 ttcatggaaa tctgcctaat gatcgtggtg taattgaagc gccgattggc cggagtgaaa
     3061 aagaccgtaa gaaacaggct gtaactgcta aagggaagcc tgcagtgacg cgttttcacg
     3121 tcttggaacg ctttggcgat tatagcttag tagagttgca actggagaca gggcgcactc
     3181 atcaaatccg tgtccacatg gcttatatcg gccattcagt cgctggtgat gaggtctatg
     3241 gtcctcgcaa gactttgaaa ggacatggac aatttcttca tgccaagact ttaggtttta
     3301 ctcatccgag aacaggtaag accttggaat ttaaagcaga tatcccagag atttttaagg
     3361 aaaccttgga gagattgaga aagtaagaat gaaaaagaaa ttaactagtt tagcacttgt
     3421 aggcgctttt ttaggtttgt catggtatgg gaatgttcag gctcaagaaa gttcaggaaa
     3481 taaaatccac tttatcaatg ttcaagaagg tggcagtgat gcgattattc ttgaaagcaa
     3541 tggacatttt gccatggtgg atacaggaga agattatgat ttcccagatg gaagtgattc
     3601 tcgctatcca tggagagaag gaattgaaac gtcttataag catgttctaa cagaccgtgt
     3661 ctttcgtcgt ttgaaggaat tgggtgtcca aaaacttgat tttattttgg tgacccatac
     3721 ccacagtgat catattggaa atgttgatga attactgtct acctatccag ttgaccgagt
     3781 ctatcttaaa aaatatagtg ataatcgtat tactaattct gaacgtctat gggataatct
     3841 gtacggttat gataaggttt tacagactgc tgcagaaaaa ggtgtttcag ttattcaaaa
     3901 tatcacacaa ggggatgctc attttcagtt tggggacatg gatattcagc tctataacta
     3961 tgaaaatgaa accgattcat cgggtgaatt aaagaaaatt tgggatgata attccaattc
     4021 cttgattagt gtggtaaaag tcaatggcaa gaaaatttac cttgggggcg acttagataa
     4081 tgttcatgga gcagaagaca agtatggtcc tctcattgga aaagttgatt tgatgaagtt
     4141 taatcatcat ttagatacaa aaaattctaa tacaaaaaat tttataaaaa atttgtctcc
     4201 tgagataatt gttcaaactt cctcctcacc aattggttcg tatgatagcg gtcaagaaat
     4261 aaatagggaa tatattagtt ggcttgaatc aatgggaatt caacatataa atgcagccag
     4321 caaagactat gatgcaacag tttttgatat tcgacaagac ggtttaataa acatttcaac
     4381 ttcctacaag cctattccaa gttttcaagc tggttggcat aagagttcat atggaaactg
     4441 gtggtatcaa gcgcctgatt ctacaggaga atatgctgtt ggttggaatg aaattaaggg
     4501 tgaatggtat tactttaacc aaacgggtat cttattacag aatcaatgga aaaaatggaa
     4561 caatcgttgg ttctatttga cagactctgg tgttgctgct aaaaattgga agaaaatcgc
     4621 tggaatctgg tattatttta acaaagaaaa ccagatggaa attggttgga ttcaagataa
     4681 agagcagtgg tattatttgg atgttgatgg ctctatgaag acaggttggc ttcaatataa
     4741 ggggcaatgg tattactttg ctccatcagg ggaaatgaaa acgggctggg taaaagataa
     4801 agaagcctgg tactatatgg attctactgg tatcatgaag acaggtgaga tagaagttgc
     4861 tggacatcat tactatctag aagaatcagg agctatgaag caaggctggc ataaaaaggc
     4921 aaatgattgg tatttctaca agacagacgg ttcacgagct gtgggttgga tcaaggacaa
     4981 ggataaatgg tacttcttga aagaaaatgg tcaattactt gtgaacggta agacaccaga
     5041 aggctatact gtggattcaa gtggtgcctg gttagtggat gtttcgatcg agaaatctgc
     5101 tacaattaaa actacaagtc attcagaaat aaaagaatcc aaagaagtag tgaaaaagga
     5161 tcttgaaaat aaagaaacga gtcaacatga aagtgttaca aattcttcaa ctagtcaaga
     5221 tttgacatcc tcaacttcac aaagctctga aacgagtgta aacaaatcgg aatcagaaca
     5281 gtagtagaaa agaaggtttt agggccttct ttttcctatc aactcttttc tatttcctgt
     5341 tattcatgtt ataatggata aatatgaata atcggagtga gactatgaaa tacaaacgga
     5401 ttgtctttaa ggtgggtact tcttctctga caaatgagga tggaagttta tcacgtagta
     5461 aggtaaagga tattacccag cagttggcta tgctgcacga ggctggtcat gagttgattt
     5521 tggtgtcttc aggtgccatt gcggctggtt ttggagcctt aggatttaaa aagcgtccga
     5581 ctaagattgc tgataaacag gcttcagcag cggtagggca agggcttttg ttggaagaat
     5641 atacaaccaa tcttctcttg cgtcaaatcg tttctgcaca aatcttgctg acccaagatg
     5701 attttgtgga taagcgtcgt tataaaaatg cccatcaggc tttgtcggtt ttactcaatc
     5761 gtggggcaat tcctatcatc aatgagaatg atagtgtcgt tattgatgag gtcaaggttg
     5821 gggacaatga cactctaagt gcccaagtag cggcgatggt ccaagcagac cttttggttc
     5881 tcttgacaga tgtggacggt ctctatactg gaaatcctaa ttcagatcca agagccaaac
     5941 gcttggagag aatagagacc atcaatcgtg agattattga tatggctggt ggagctggtt
     6001 cgtcaaacgg aactgggggt atgttaacca aaatcaaggc tgcaactatc gcgacggaat
     6061 caggagttcc tgtttatatc tgctcatcct tgaaatcaga ttccatgatt gaggcggcag
     6121 aggagaccga ggatggttct tactttgttg ctcaagagaa ggggcttcgt acccagaaac
     6181 aatggcttgc cttctatgct cagagtcaag gttctatttg ggttgataaa ggggctgcgg
     6241 aagctctctc tcaacatgga aagagtcttc tcttatctgg tattgttgaa gcagaaggag
     6301 ccttttctta cggtgatatc gtgacagtat ttgacaagga aagtggaaaa tcacttggaa
     6361 aaggacgcgt gcaatttgga gcatctgctt tggaggatat attgcgttct caaaaagcca
     6421 agggtgtctt gatttaccgt gacgactgga tttccattac tcctgaaatc caactacttt
     6481 ttacagaatt ttagaggtaa actatggtga gtagacaaga acaatttgaa caggtacagg
     6541 ctgttaaaaa atcgattaac acagctagtg aagaagtgaa aaatcaagcc ttgctagcca
     6601 tggctgatca cttagtagct gctactgagg aaattttagc ggctaatgcc ctcgatatgg
     6661 cagcggctaa ggggaaaatc tcagatgtga tgttggatcg tctttatttg gatgcagatc
     6721 gtatagaagc gatggcaaga ggaattcgtg aagtggttgc cttaccagat ccaatcggtg
     6781 aagttttaga aacaagtcag cttgaaaatg gtttggttat cacaaaaaaa cgtgtagcta
     6841 tgggtgtcat cggtattatc tatgaaagcc gtccaaatgt gacgtctgat gcggctgctt
     6901 tgactcttaa gagtggaaat gcggttgttc ttcgtagtgg taaggatgcc tatcaaacaa
     6961 cccatgccat tgtcacagcc ttgaagaagg gcttggagac gactactatt cacccaaatg
     7021 tgattcaact ggtggaagat actagccatg aaagtagtta tgctatgatg aaggccaagg
     7081 gctatctaga cctcctcatt cctcgtggag gagctggctt gatcaatgca gtggttgaga
     7141 atgcgattgt acctgttatc gagacaggga ctgggattgt ccatgtctat gtggataagg
     7201 atgcagacga agacaaggcg ctgtctatca tcaacaatgc taaaaccagt cgtccttctg
     7261 tttgtaatgc catggaggtt ctgctggttc atgaagataa ggcagcaagc atccttcctc
     7321 gcttggatca aatgctggtt gcagagcgta aggaagctgg actggaacca attcaattcc
     7381 gcttggatag caaagcaagc cagtttgttt caggtcaagc agctgagacc caagactttg
     7441 acaccgagtt tttagactat gtccttgctg ttaaggttgt gagcagttta gaagaagcgg
     7501 ttgcgcacat tgaatcccac agcacccatc attcggatgc tattgtgacg gaaaatgctg
     7561 aagctgcagc atactttaca gatcaagtgg actctgcagc ggtgtatgtt aatgcctcaa
     7621 ctcgtttcac agatggagga caatttggtc ttggttgtga aatggggatt tctactcaga
     7681 aattgcacgc gcgtggtcca atgggcttga aggaattgac tagctacaag tatgtggtca
     7741 ctggtgatgg acagataagg gagtaagaga tgaagattgg atttatcggt ttggggaata
     7801 tgggtgctag tttagccaag tctgtcttgc aggctagacc gtcagatgag attctccttg
     7861 ccaatcgtag tcaagtcaag gtggatgctt tcatcgctaa cttcggtggt cagacttcta
     7921 acaatgaaga aatattcgaa gaagcagatg tgatttttct aggtgttaag cctgctcagt
     7981 tttctgaact gctttctcaa taccagacca tccttgaaaa aagagaaagt cttcttttga
     8041 tttcgatggc agctggattg atcttagaaa aactagcaag tcttatccca agccaacacc
     8101 gaattattcg tatgatgcct aatacccctg cttctatcgg gcaaggagtg attagttatg
     8161 ccttgtctcc taattgcagg gctgaggaca gtgagctctt ttgtcagctt ttatccaagg
     8221 ctggtctctt ggttgaatta ggagaaagct taatcaatgc agcgacaggt cttgcaggct
     8281 gtggaccagc ctttgtctat ctctttattg aggccttggc agatgcaggt gttcagacgg
     8341 gattaccacg agaaacagcc ttgaaaatgg cagcccaaac tgtggtagga gctgggcaat
     8401 tggtcctaga aagccagcaa catcctggag ttttgaaaga tcaagtttgt agtccaggcg
     8461 gttcgactat tgctggcgta gcaagcctag aagcgcatgc ttttcgagga acggtcatgg
     8521 aggcagttca tcaagcctat aaacgaacac aagaactagg taaataagag gtagttttat
     8581 ctgcctcttt tatggtggtt gatactcttc gaaaatcaaa ttcaaactgc gtcagcttca
     8641 ccttgccgta ctcaagtaca gcctgcggct agcttcctag tttgctcttt gattttcatt
     8701 gagtatgaaa tgagaagaca aaaagattgt cacaaacccc tatttttttg atagaataga
     8761 agtagtaaaa aagaaatgag ttagacatgt caaaaggatt tttagtctct cttgagggac
     8821 cagagggagc aggcaagacc agcgttttag aggctctgct accaatttta gaggtaaaag
     8881 gggtaaaggt gctgacgacc cgtgaacctg gcggagtctt gattggggag aagattcggg
     8941 aagtgatttt ggatccaagt catactcaga tggatgctaa aacagagcta cttctctata
     9001 ttgccagtcg cagacagcat ttggtggaaa aagttcttcc agcccttgaa gctggcaagt
     9061 tggtcatcat ggatcgtttt atcgatagtt ctgttgccta tcagggattt ggtcgtggct
     9121 tagatattga agccattgac tggctcaatc agtttgcgac agatggcctc aaacccgatt
     9181 tgacactcta ttttgacatc gaggtggaag aagggctggc tcgtattgct gctaatagtg
     9241 accgcgaggt taatcgtttg gatttggaag ggttggactt gcataaaaaa gttcgtcaag
     9301 gctacctttc tctcctggaa aaagaaggaa atcgcattgt caagattgat gctagtctcc
     9361 ctttggagca agttgtggaa actaccaagg ctgtcttgtt tgacggaatg ggcttggcca
     9421 aatgaaacaa gatcaactaa aggcttggca accagctcag tttgaccgtt ttgtccgtat
     9481 cttagaacaa gaccagctca atcacgccta tctcttttca ggtttctttg gaagcttgga
     9541 aatggcgcaa tttttagcta agagcctctt ttgtacggat aaagttggcg tcttaccatg
     9601 tgagaaatgc cgaagttgca agctgattga acaggaagag tttccagatg tcaccttgat
     9661 taagccagtc aatcaggtca tcaagacaga acgcattcgg gaattggtgg gacagttttc
     9721 tcaagcaggg attgaaagcc agcaacaggt ctttatcatc gagcaagcgg ataaaatgca
     9781 tcccaacgca gccaattctc tgctcaaggt catcgaagaa ccccagagtg aagtttatat
     9841 tttcttcttg actagcgatg aggaaaagat gttaccgaca atccgaagtc ggactcagat
     9901 cttccacttt aaaaagcaag aagaaaaact tatcttactc ttagaacaaa tgggacttgt
     9961 taagaaaaaa gcgactcttt tagctaagtt tagtcaatcg cgagctgaag cagaaaagtt
    10021 ggctaatcag gcaagttttt ggaccttggt cgatgaaagt gaacgcctgc tgacttggtt
    10081 agtagctaag aaaaaagaaa gttatctaca ggttgccaaa ttagccaact tggcagatga
    10141 taaggaaaaa caggatcagg ttttacggat tcttgaagtt ctctgtgggc aggacctctt
    10201 gcaggtaaga gtaagagtga ttctacaaga tttactagaa gctagaaaaa tgtggcaagc
    10261 taatgtcagc tttcaaaatg ccatggaata tctggtcttg aaagaaatat aaactcaaaa
    10321 atgaatgata aagaaaggaa agggctgttt tatggacaaa aaagaattat ttgacgcgct
    10381 ggatgatttt tcccaacaat tattggtaac tttagccgat gtggaagcca tcaagaaaaa
    10441 tctcaagagc ttggtagaag aaaatacagc tcttcgcttg gaaaatagta agttgcgaga
    10501 acgcttgggt gaggtggaag cagacgctcc cgtcaaggcc aagcatgttc gcgaaagtgt
    10561 ccgtcgtatt taccgtgatg gatttcacgt atgtaatgat ttttatggac aacgtcgaga
    10621 gcaggacgaa gaatgtatgt tctgtgatga gttgttatac agggagtaag catgcagatt
    10681 caaaaaagtt ttaaggggca gtctccctat ggcaagctgt atctagtggc aacgccgatt
    10741 ggcaatctag atgatatgac ttttcgtgct atccagacct tgaaagaagt ggactggatt
    10801 gctgctgagg atacgcgcaa tacaggtctt ttgctcaagc attttgacat ttccaccaag
    10861 cagatcagtt ttcatgagca caatgccaag gaaaaaattc ctgatttgat tggtttcttg
    10921 aaagcagggc aaagtattgc tcaggtctct gatgccggtt tgcctagcat ttcagaccct
    10981 ggtcatgatt tggttaaggc agctattgag gaagaaattg cagttgttac agttccaggt
    11041 gcctctgcag gaatttccgc cttgattgcc agtggtttag cgccgcagcc acatatcttt
    11101 tacggctttt taccgagaaa atcaggccag caaaagcaat ttttcgactc gaaaaaagac
    11161 tatcctgaaa ctcagatttt ttatgaatcg ccccatcgtg tggcagatac attggaaaat
    11221 atgctagaag tctacggtga ccgctcggta gtcttggtta gagaattgac caaaatctat
    11281 gaagaatacc aaagaggtac aatttctgaa ttgctggaaa gcatctctga aacgtctctc
    11341 aagggtgaat gtcttctgat tgttgaaggt gccagcaaag gtgtggagga aaaggatgag
    11401 gaagacttgt tcttagaaat ccaagcccgt atccagcaag gcatgaagaa aaatcaagct
    11461 attaaggaag tcgctaagat ttaccagtgg aataaaagtc agctctacgc tgcctaccac
    11521 gactgggaag aaaatcaaga aaatgactaa acagggaagt ttgccttctt cccttttttt
    11581 gttatactag tagaagaaaa aattagaaag atttgtgggt gttaaacaag ccagtgcctt
    11641 gttttgatat gaacttaggt tccacccaaa gaggtattag tgtcgtgtct caatcttata
    11701 tcaatgttat cggtgctggt ttggcaggtt ctgaagcagc ttaccaaatc gcagagcgtg
    11761 gtattccagt taaactatat gaaatgcgtg gtgtcaagtc tacaccccag cataaaacag
    11821 acaattttgc tgagttggtt tgttccaatt ctttgcgtgg ggatgctttg acaaatgcag
    11881 ttggtcttct caaggaagaa atgcgtcgct tgggttctgt tatcttggaa tctgctgagg
    11941 ctacacgtgt tcctgccggt ggagcccttg cggtggatcg tgatggtttc tctcaaatgg
    12001 tgaccgaaaa agttgccaac caccccttga ttgaagtggt tcgtgatgaa attacagaat
    12061 tgccgacaga tgttattacg gttatcgcta ctggtccttt gacaagtgat gccttggctg
    12121 aaaagattca tgcccttaat gacggtgatg gtttttattt ctacgatgcg gcagcgccta
    12181 ttatcgatgt caacactatc gatatgagca aggtctacct caaatcacgt tatgataagg
    12241 gagaagcggc ctacctcaat gcccctatga ccaagcaaga atttatggat ttccatgaag
    12301 ctttggtcaa tgcagaagaa gcaccgctta attcttttga aaaagaaaag tactttgaag
    12361 gatgtatgcc tatcgaagtt atggccaaac gtggcattaa aactatgctt tatggcccta
    12421 tgaagccagt cggtcttgag tatccggacg actacacagg acctcgtgat ggagaattta
    12481 aaacacctta tgcggttgtg caacttcgtc aggataatgc agctggtagc ctctacaata
    12541 tcgttggttt ccagacccac ctcaaatggg gagaacaaaa gcgtgtcttc caaatgattc
    12601 cgggtcttga aaatgcggag tttgtccgtt atggtgtcat gcatcgcaat tcttacatgg
    12661 attcaccaaa tcttcttgag cagacttacc gttccaagaa acaaccaaat ctcttctttg
    12721 ctggtcagat gacgggggtg gaaggctatg ttgagtcggc ggcttcgggc ttagttgcgg
    12781 gaattaacgc agctcgtctc ttcaaggaag aaagcgaggt tattttcccc gagacgacag
    12841 cgattggaag cttagctcat tacattaccc atgccgacag caaacatttc caaccaatga
    12901 atgtcaattt tgggatcatc aaggagttgg aaggcgagcg tatccgtgat aagaaggctc
    12961 gttatgaaaa aattgcagag cgtgcccttg ccgacttaga ggaatttttg actgtctaat
    13021 ttttttgaaa gaattgctca tgatactata aaaatcttag aaattgtgat aaaataggta
    13081 ggatgaaaga aggagagtga aaatggcgaa tcccaagtat aaacgtattt taatcaagtt
    13141 atcaggtgaa gcccttgccg gtgaacgtgg cgtagggatt gatatccaaa cagttcaaac
    13201 aatcgcaaaa gagattcaag aagttcatag cttaggtatc gaaattgcgc ttgttattgg
    13261 tggaggaaat ctctggcgtg gagaacctgc agcagaagca ggtatggacc gtgttcaggc
    13321 agattacact ggaatgcttg ggactgttat gaatgctctt gtgatggcag attcattgca
    13381 acaagttggg gttgatacgc gtgtacaaac agctattgct atgcaacaag tggcagagcc
    13441 ttatgtccgt ggacgtgccc ttcgtcacct tgaaaaaggc cgtatcgtta tctttggtgc
    13501 tggaattggt tcaccatact tctcgacaga tacaacagcg gcccttcgtg cagctgaaat
    13561 cgaagcagat gccatcctca tggctaaaaa tggcgtcgat ggtgtgtaca atgccgatcc
    13621 taagaaggac aagacagccg ttaagtttga agaattgacc caccgtgatg ttatcaacaa
    13681 aggtcttcgt atcatggact caacagcctc aaccctctca atggacaacg acattgactt
    13741 ggttgtcttc aacatgaacc aatcaggcaa catcaaacgt gtcgtatttg gtgaaaatat
    13801 cggaacaaca gtttcaaata atatcgaaga aaaggaataa gaaagaatat ggctaacgta
    13861 attattgaaa aagctaaaga gagaatgacc cagtctcacc aatcacttgc tcgtgaattt
    13921 ggtggtatcc gtgctggtcg tgccaatgca agcttgcttg accgtgtaca tgtagaatac
    13981 tatggagtcg aaactcctct taaccaaatc gcttcaatta cgattccaga agcgcgtgtt
    14041 ttgttggtaa caccatttga caagtcttca ttgaaagaca tcgaacgtgc cttgaacgct
    14101 tctgatcttg gtatcacacc ggctaatgac ggttctgtga ttcgcttggt tatcccagct
    14161 cttacagaag aaactcgtcg tgaccttgct aaagaagtga agaaggtcgg cgaaaatgct
    14221 aaagtggctg tccgcaatat ccgtcgcgat gctatggacg aagctaagaa acaagaaaaa
    14281 gcaaaagaaa tcactgaaga cgaattgaag actcttgaaa aagatattca aaaagtaaca
    14341 gacgatgctg ttaaacacat cgacgatatg actgccaaca aagagaaaga acttttggaa
    14401 gtctaaaaat aaacagaaaa actcagttgg cattgctggc tgagttttat tcgaaagaag
    14461 gaaatatgaa tacaaatctt gcaagtttta tcgttggact gatcatcgat gaaaacgacc
    14521 gtttttactt tgtgcaaaag gatggtcaaa cctatgctct tgctaaggaa gaaggccaac
    14581 atacagtagg ggatacggtc aaaggttttg catacacgga tatgaagcaa aaactccgcc
    14641 tgacaacctt agaagtgact gccactcagg accaatttgg ttggggacgt gtcacagagg
    14701 ttcgtaagga cttgggtgtc tttgtggata caggccttcc tgacaaggaa atcgttgtgt
    14761 cactcgatat tctccctgag ctcaaggaac tctggcctaa gaagggcgac caactctaca
    14821 tccgtcttga agtggataag aaagaccgta tctggggtct cttggcttat caagaagact
    14881 tccaacgtct tgctcgtcct gcctacaaca acatgcagaa ccaaaactgg ccagccattg
    14941 tttaccgtct caagctatca ggaacttttg tttacctacc agaaaataat atgcttggtt
    15001 ttattcatcc tagcgagcgt tacgcagagc cacgtttggg gcaagtatta gatgcgcgcg
    15061 ttattggttt ccgtgaagtg gaccgcactt tgaacctctc cctcaaacca cgctcctttg
    15121 aaatgttgga aaacgatgct cagatgattt tgacttattt ggaaagcaat ggcggtttca
    15181 tgaccttaaa tgacaagtca tctccagacg acatcaaggc aacctttggc atttctaaag
    15241 gtcagttcaa gaaagcttta ggtggtctta tgaaggctgg taaaatcaag caggaccagt
    15301 ttggtacaga gttgatttag ggaggcttat gagaaaatca ttttacactt ggctcatgac
    15361 cgagcgcaat cctaaaagta acagtcccaa agcaattttg gcagacctcg cttttgaaga
    15421 gtcagccttt ccaaaacata cagatgattt tgatgaggtc agtcgctttt tggaggagca
    15481 tgccagtttc tcttttaacc taggagattt tgacagcatt tggcaggaat atctagaaca
    15541 ctagcattta ttcattgggt ttgggctagt aatttctcca tccctctgct ataataaaaa
    15601 gaaataaaag gattagagag gttctttatt tgaaggaaca ttcaatagac attcaactga
    15661 gtcatccaga tgacctgttt catctttttg gttccaatga acgccatctt cgtttgatgg
    15721 aagaagagct tgatgttgtg attcatgctc gtacggagat tgtccaggtt ttgggagaag
    15781 agtctgcctg tgaggaagcc cgtcaagtta ttcaggcttt gatggtcttg gtaaatcgtg
    15841 ggatgaccgt tggtacgcca gatgtagtca ctgcgattag catggtcaaa aatgatgaaa
    15901 ttgacaagtt tgtcgccctt tacgaagaag aaattatcaa agataatact gggaaaccta
    15961 tccgtgtcaa aaccctaggg caaaagcttt atgtggacag tgtcaaacag catgatgtga
    16021 cctttggaat tgggccagca ggtacaggga agaccttcct tgcagtgacc ttggcagtga
    16081 ctgcccttaa acgtgggcaa gtcaagcgaa ttatcctaac tcgtccagcg gtggaagcgg
    16141 gagagagtct tggatttctt ccgggtgatc ttaaggagaa ggtggatcct taccttcgtc
    16201 ctgtttacga tgccttgtat caaattcttg ggaaagacca aacgactcgt ctcatggagc
    16261 gtgaaattat cgaaattgcg ccccttgcct atatgcgtgg tcggaccttg gatgatgcct
    16321 ttgtcattct cgatgaggcg caaaatacga ccatcatgca gatgaagatg ttcttgactc
    16381 gtttaggttt tcattctaag atgattgtca atggagatat tagtcagatt gatctgccac
    16441 gtaatgtcaa gtccggtttg attgatgccc aagaaaagct caagaacatt taccaaattg
    16501 actttgttca tttttcagct aaggatgtgg ttcgccatcc agttgtcgct cagattatcc
    16561 gagcttatga accagctcca gttaaaaaag aagagagtga agaattagag tctcaacctc
    16621 tgtctgaagg atagttcttt tgtacttaaa gttatctcaa tgctaagtat aaatt
//
LOCUS       DAVLTM010000037        16430 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLTM010000037 DAVLTM010000000
VERSION     DAVLTM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.037"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(242..952)
                     /gene="treR"
                     /locus_tag="VYN16_001024"
     CDS             complement(242..952)
                     /gene="treR"
                     /locus_tag="VYN16_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1403571.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1137..3104
                     /gene="treP"
                     /locus_tag="VYN16_001025"
     CDS             1137..3104
                     /gene="treP"
                     /locus_tag="VYN16_001025"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1403572.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFVYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            3282..4907
                     /gene="treC"
                     /locus_tag="VYN16_001026"
     CDS             3282..4907
                     /gene="treC"
                     /locus_tag="VYN16_001026"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1403573.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQTLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5163..5411
                     /locus_tag="VYN16_001027"
     CDS             5163..5411
                     /locus_tag="VYN16_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1403574.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5618..6412
                     /gene="racE"
                     /locus_tag="VYN16_001028"
     CDS             5618..6412
                     /gene="racE"
                     /locus_tag="VYN16_001028"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1403575.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6409..7380
                     /locus_tag="VYN16_001029"
     CDS             6409..7380
                     /locus_tag="VYN16_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1403576.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7356..7877
                     /locus_tag="VYN16_001030"
     CDS             7356..7877
                     /locus_tag="VYN16_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1403577.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            7874..8335
                     /locus_tag="VYN16_001031"
     CDS             7874..8335
                     /locus_tag="VYN16_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1403578.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8326..9066
                     /gene="xerD"
                     /locus_tag="VYN16_001032"
     CDS             8326..9066
                     /gene="xerD"
                     /locus_tag="VYN16_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1403579.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            9066..9794
                     /locus_tag="VYN16_001033"
     CDS             9066..9794
                     /locus_tag="VYN16_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1403580.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            9813..10382
                     /locus_tag="VYN16_001034"
     CDS             9813..10382
                     /locus_tag="VYN16_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1403581.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10369..11091
                     /locus_tag="VYN16_001035"
     CDS             10369..11091
                     /locus_tag="VYN16_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1403582.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            11091..11333
                     /gene="yidD"
                     /locus_tag="VYN16_001036"
     CDS             11091..11333
                     /gene="yidD"
                     /locus_tag="VYN16_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1403583.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(11558..12523)
                     /locus_tag="VYN16_001037"
     CDS             complement(11558..12523)
                     /locus_tag="VYN16_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1403584.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(12619..13371)
                     /locus_tag="VYN16_001038"
     CDS             complement(12619..13371)
                     /locus_tag="VYN16_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403585.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(13368..14324)
                     /locus_tag="VYN16_001039"
     CDS             complement(13368..14324)
                     /locus_tag="VYN16_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1403586.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(14314..15273)
                     /locus_tag="VYN16_001040"
     CDS             complement(14314..15273)
                     /locus_tag="VYN16_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403587.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKCYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(15696..16397)
                     /locus_tag="VYN16_001041"
     CDS             complement(15696..16397)
                     /locus_tag="VYN16_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1403588.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLKENQIDAVLHYAGEIVVSESIENPSKYFTANVAGRNQV
                     LKVLSEVGTQKIMFSSTASLYGNNCIDKPATEDTLIDPVNPYTETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         4601 a         3277 c         3793 g         4759 t
ORIGIN      
        1 gtgtgtatcc ttcttgatga tgtctaaaat ctggatatta gggtctttaa tgtctagtaa
       61 ttttgtgata aaatgtaatt gttccatatg attctttcta atgagttgtt ttgtcgcttt
      121 tcattatagg tcatatggga ctttttttct acacaaaata ggctccataa tatccatagg
      181 ggggttaccc actacaaata ttatagagcc gaaaaaggct gagatctggt gtctcagtct
      241 tttatttctt gcgttttgca aaatctacaa atctaaattt ttctaactta tggcgacttt
      301 cggtaaactg aaattggcgt ccgtcttgga gatagacttt tgatttaatc gaaacgacat
      361 aagggtcttt gccaatgtcc atgagaatct tgtctcggtc gcttgagtgg tcaatggtga
      421 tttccttctg agcataatca ataaggagtt tgaggccatt ttctatataa gaatagatag
      481 actgctcagc aatttggcga gtgagatttg gaattagttc catatccaga tagtctgtat
      541 ccagaacgga taccagatca tccaccacac gctggcggac caccttccaa accatccgaa
      601 actctgggaa accggttatc agtgaggatt ttttatcaat aataatcttg tccagactga
      661 ccacgttggt tttagagcgc agtccaagtt ctttaactag ttcttggtag ctggttaggt
      721 tggatacagg gaaattgacg gtttcttctt tgacgacttg agaaccttgc cctcttatct
      781 ttttgatcaa tccttcctct tggaggagag acagggcctt tcggacggta tcacgactca
      841 cttgatattg ctccataagg tcgtgctcgc taggaaggaa atctccgaca gcgtaagtct
      901 cgttttgaat ggtttcttgg atttgcttaa ataattgttg gtatttcttc attttgcctg
      961 cttaaatagg tttttagtct agtttaatga acacctgtat ctattttacc atacaagtgc
     1021 tagaaaattt acaaaaaaac acccttttta taccaaacaa gttgcagaca agtttggtat
     1081 cgtttacaat atacttatca aatcaaactt gctttgacaa gtataaggag aatcaaatgg
     1141 gaaaatttga acaagaagcc aaagatctgc ttcaggcaat tggaggcaaa gaaaatgtga
     1201 ctgccgtcac ccactgtgcg acacggatgc gctttgtttt aggagatgat aagaaggcta
     1261 atgttaaagc tatcgagtca attccagctg ttaaaggaac ttttacaaat gcaggtcaat
     1321 ttcaggtaat cattggaaat gacgtgccca tcttttataa tgattttaca gccgtttcag
     1381 gtattgaggg tgtttccaaa gaagcagcca agtctgcagc taagagtaat caaaacgtgg
     1441 tccaacgtgt tatgaccact ctggcggaga tttttactcc gattattcca gccttgatag
     1501 tcggaggatt gatcctcggt ttccgtaatg tcttggaagg tgtccattgg tcgatgttgg
     1561 atggcaagac catcacagaa tcctctcagt tttgggcagg tgtcaatcac ttcctctggt
     1621 tgcctggtga agctatcttc cagttcttac cagtagggat tacttggtct gtttctcgta
     1681 agatgggaac cagccaaatt ttgggaattg ttctcggaat ctgtttggta tcgcctcagt
     1741 tgctcaatgc ctatgcggtt gcttcaacgc cagcagctga tatcgcggca aactgggttt
     1801 ggaattttgg ctattttact gttaatcgta tcggttacca agcccaagtt atcccagcct
     1861 tgcttgcagg tttgagtctg tcttatcttg aaatcttctg gcgcaagcat atcccagaag
     1921 tcatttctat gatttttgta cctttcttgt cattgattcc agccttgatt ttggctcata
     1981 ctgttttggg accaatcggt tggacaattg gacaaggact ttcatcagtt gtcttggcag
     2041 gtttaactgg tccagttaaa tggctcttcg gtgcaatttt tggtgccctc tacgctccat
     2101 ttgtcatcac aggtctgcac catatgacca atgccattga tacacaattg attgcggatg
     2161 ctggtggcac tgccctatgg ccaatgattg ctctttctaa tattgctcaa ggctcagccg
     2221 tgtttgtcta ttatttcatg catcgccatg atgagcgtga ggctcaggtt tcacttcctg
     2281 caaccatttc agcctatctc ggtgttacag aaccagctct ttttggggtt aacgtaaaat
     2341 atatttatcc atttgttgct gggatgactg gttcagccct tgcaggcatg ttatccgtta
     2401 cttttaatgt aactgcggct tctattggta tcggtggttt gccaggtatt ctctctattc
     2461 aacctcaata catgctgcca tttgcaggaa ctatgctagt tgcgattgtt gttccaatgc
     2521 tcttgacttt cttcttccgc aaggctggtc tctttacaaa aacagagggc gatacgaact
     2581 tgcaggcaga attcgttgct caagaagaag cagaatttgt gaaccatgaa ccagtagaac
     2641 ttacttcggt agaaattatc agcccactaa ctggccaagt gaaagaattg agtcaagcga
     2701 cggatcctat ttttgcatca ggtgtcatgg ggcaaggtct agtcattgaa ccaagccaag
     2761 gtgagttgac ctctccagtt aatgggacag tgacggttct tttccctacc aagcatgcca
     2821 tcggcattgt ctctgacgag ggagttgaat tgctcatcca catcggtatg gatacagtag
     2881 gtcttgatgg caaaggtttt gaaagtcttg tagtccaagg agatcacgtt acagttggtc
     2941 agcaactgat tcgttttgat atggatgtca ttaaggctgc aggtctggtg acagaaactc
     3001 ctgttatcat caccaaccaa gatgcttata cagcgactat tcccggaact tatccgacaa
     3061 cgatccaagc tggagcatct ctcatggtcg ctacacgaat ctaatactct tcgaaaatct
     3121 cttcaaacca cgtcagctct atctgcaacc tcaaaatagt gttttgagca acctgtggct
     3181 agcttcctag tttgttcttt gattttcatt gagtataatt gaaatagcac aaatagggac
     3241 ggaagactgc ttcctccctt ttatcagaaa aggagaaaca gatgacactt gataaaggaa
     3301 aagtagtcta tcaaatctat ccaaaatctt acaaagacac cactgaaaac ggtattgggg
     3361 atttccgtgg gattatcgaa aaaattccct atttagccaa actaggtgtg gatatggtct
     3421 ggctcaatcc attctatcca agccctcaac gggataatgg ttacgatatt tcagattata
     3481 tggcagtgga tcctcttttt ggtgatatgg ctgattttga ggaaatggtg tgtgtcggta
     3541 aagagcacaa gattgacttt atgctggaca tggtacttaa tcattgttcg acagagcatg
     3601 aatggtttca gaaagcccta gctggtgata agtattatca agactttttc ttcatccaag
     3661 accaaccaac agattggcag tctaagtttg gtggttctgc atggacgcct ttcggggata
     3721 ctgggaaata ctatcttcac ctatttgatg agacccaggc tgaccttaac tggcgcaatt
     3781 ccaatgtccg taaggagctt ttcaaggttg ttaatttctg gcgcgacaag ggtatcaaag
     3841 gtttccgatt tgatgtgatc aatttgatcg gtaaggacga ggtttcagtg gactgtcctg
     3901 aaaatgaagg gaagccagct tatacagaca aacccatcgt tcataactat ttgcgtatga
     3961 tgaaccaagc cacttttgga tctgacgata gctttatgac agttggggaa atgtcttcta
     4021 ccaccatgga aaactgtgtc ctctattcgt cgcctgatcg tcaggaatta tccatgacct
     4081 ttaattttca tcacctcaag gtggactata aggatggaca aaagtggacc ttggctccct
     4141 ttgattttga agagttgaag agtctttacc atagttgggg taaggaaatg agtgataaag
     4201 acggttggag tgccctcttt tggaacaatc acgatcaacc acgtgccttg aaccgttttg
     4261 tcgatattca aaacttccgc aaggaaggag ccaccatgct agcggctagc attcacctgt
     4321 cacgtgggac accttatatc tatatgggtg aagaaatcgg gatgattgac ccagactatg
     4381 attctatggc tgattatgtg gatgtcgaat cgcttaatgc ctatcagacg ctcttagaag
     4441 aaggaaagag tcagcaagaa gccttccaga ttattcaagc taagtcgcgt gataattcac
     4501 gcattcccat gcagtgggat gcttcggaaa atgcaggatt ttcaacaggc actccatggt
     4561 tgaaagctgg taaatcctat aaatacatca atgtagaaaa tgaaatccag ggtccgattt
     4621 tcaccttcta tcaagacttg attcgactgc gtaaggaaat gccaatcatt tcagaaggaa
     4681 gttacaaacc agcctttgaa gatagcaaac aagtctacgc ttttgaacgc cagtttgagg
     4741 atcaaaagtt actagtgctc aataactttt atgctaaaga agtggaaatc gacttgccag
     4801 cagtctacca aaatggacag attttgatat caaactatga agatgcagaa gtgtctgaga
     4861 aaattctact caaaccctat caaacactgg ctatttatgt aaattaatac tcttcgaaaa
     4921 tctcttcaaa ccgtgtcagt tttatctgca acctcaaagc ggtgctttga gcaacctgtg
     4981 gctagcttcc tagtttgctc tttgattttc attgagtata aacttagcaa ggagacctct
     5041 ttttcagaaa agcagggttc tttttgctgt ctattccatc cctgaaagtg tagaacaaac
     5101 tggtcagggt ggctagtttg tggtataatg aaagggattc aaaaagaaac aggagaaaaa
     5161 tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga ggagctcttg
     5221 gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac ccacgtttga
     5281 atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc gaagctaagg
     5341 tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct aacaataaaa
     5401 agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac tatgttggtt
     5461 atggtttagg gatgagactt tttccttgca ttctattgat atttgattat gattaagaga
     5521 gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg aatcataatg
     5581 aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac caattggttt
     5641 tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc agcttcccca
     5701 tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc gtcctgctga
     5761 gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg atgtcaaaat
     5821 gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca aggctcaact
     5881 agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca agtccagtca
     5941 aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca tataccgtca
     6001 gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc ccaagtttgc
     6061 tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg tctatgaaac
     6121 cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc attatccact
     6181 ccttcgccct attatccaaa atgtgatggg gccaaaggtt cagctcatcg atagtggggc
     6241 agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc gtggtcgcga
     6301 tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt ttgcacaaat
     6361 tggtgaagaa tggctggaaa aagagattca tgtggagcat gtagaattat gacaaataaa
     6421 atttatgaat ataaggatga ccaggactgg tatgttgggt cttatagtat ttttggtggc
     6481 gttaacagtt tgagcgacta tcagacagat tttcctctgt ttgaattctc caaaatattt
     6541 ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg ttctacctac
     6601 cgtttgttct cctttgtggt agatatgctt aatcaagaaa tgggacgaaa cttggaagtt
     6661 attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta tgtagaattg
     6721 cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaagtcag agaaaccttg
     6781 ttgattgcga ctcgtaacga aggtaaaacc aaggaattcc gagctatctt tgataagtta
     6841 ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc agaaacaggt
     6901 atgacctttg aggaaaatgc ccgccttaag gcagaaacca tttctcaatt aacgggcaag
     6961 atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt accaggcgtc
     7021 tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc caaactcttg
     7081 cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca cacaacccta
     7141 gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc aggttatatt
     7201 aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct tgtaggagag
     7261 acaggtgagt catcagctga attaaccctg gaagaaaaaa atagtcaatc tcaccgtgcc
     7321 ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc atcattgtaa
     7381 tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc tatgtgggca
     7441 aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct ccactttggg
     7501 agggcatccg cgttgttaaa gggaacatgg acttctacgc cggctaccca gaacgtctgg
     7561 tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt gacatcaatt
     7621 tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc tgcctctatg
     7681 gtcacttgca tgtgccaagt gcttggttgg aaggcaagat cctctttcta aatccaggct
     7741 ctatcagtca accacgaggt accatcagag aatgtctcta tgctcgtgtg gagattgatg
     7801 atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca ggtttgtcca
     7861 aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc aggaggaaac
     7921 ttttttgacc cctgctaaaa atctagctgt gttgattgat actcacaatg cggatcatgc
     7981 gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag atgaaaaaca
     8041 gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc atgacttgag
     8101 ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg tagcggttgt
     8161 ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt ccttcttacc
     8221 ggttgtggat gcagagggta ttttccaagg gattattacg cgcaagtcca tcctcaaggc
     8281 cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc aatgagagac
     8341 aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa gcagtcctat
     8401 aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga gaccagtctc
     8461 aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg aaagatttcg
     8521 gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag cttttaccgc
     8581 ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct atacctagac
     8641 tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt aatcctagaa
     8701 atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa tctggatttt
     8761 caggtgttgc gaatcagcaa ggcttcccaa cggaggattg tcaccattcc cacggccttg
     8821 ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg agggaaaccc
     8881 tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa aggttttcca
     8941 tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga aaacaaggtc
     9001 gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt agaaaaatat
     9061 agataatgga tattaaatta aaagattttg aaggacccct ggacttactc ttgcatctgg
     9121 tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc gaacagtatc
     9181 tagcctatgt ctcaaccctg caggccatgc gtctggaagt gacgggtgag tacatggtca
     9241 tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta gcagaagtga
     9301 cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa tatcgcaagt
     9361 tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag tattattcca
     9421 aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag acgaccattg
     9481 acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt gcacaaaatc
     9541 acacgactat cttgcgggat gagtataaga ttgaggacat gatgattatt gtgaaagaat
     9601 ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc cagaatgtcc
     9661 aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag gagctgatcc
     9721 tcgtgcaaga ggagagtttt ggagatatct atctcatgga aaagaaggaa gaaagtcaag
     9781 tgcctcaaag ctagacttga tagagaggaa agatgagtac tttagcaaaa atagaagcgc
     9841 tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa ctcctctctc
     9901 tgccaccgac aggcatccag caaagtttag gaaaattagc ccagaagtat gaaaaggacc
     9961 cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg accaagcctc
    10021 aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc ttgtctcggg
    10081 ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg atagaaattg
    10141 atgccatccg tggggttaac tcgagtggag ccttggcaaa gttgcaggct tttgacctga
    10201 taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg actacggatt
    10261 atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt gatgagcttg
    10321 agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat gagaatcaat
    10381 aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct gattaagcaa
    10441 ggcttggtga cggttaacgg ccaagtggtg cgtgaactag caaccactat caagtcaggc
    10501 gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggttta ctatctgctt
    10561 aacaaaccac gcggtgtgat ttccagtgtg acagatgata agggtcgcaa gacggttgtc
    10621 gacctcttgc ccaatgtcaa agaacgtatt taccctgtgg gtcgtttgga ctgggataca
    10681 tcaggtgtct tgattttgac caatgatggg gactttacag acgagatgat tcaccctcgt
    10741 aatgagattg acaaggttta tgtcgcgcgt gttaaaggtg tggccaataa ggacaatctc
    10801 cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc tgtttatgaa
    10861 attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat ccatgaaggg
    10921 cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga taagttgtct
    10981 cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc ccgtcgtctt
    11041 aataaaaaag aaatcagcca actacacacc atggctgtaa ctaagaaata atgaaacgaa
    11101 ttttaatagc gcctgtgcgc ttttaccaac gttttatctc accagtcttt ccaccctctt
    11161 gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa catggtttta
    11221 agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg aaaacaggta
    11281 aggaccccgt tccagaccac ttttccctta aacgaaatca agaaggggaa tgaggtgggg
    11341 taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtgaac ttgataggat
    11401 gcgttttaga atgtcaaaat tttatactct tcgaaaatcc cttcaaaccg cgtcagcttt
    11461 catctgcaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctctt
    11521 tgattttcat tgagtattaa attgagtttg aagtggctta tttcaaagct ttttgtatgt
    11581 cttcaatcat gagttttgtt gattcaagtc cgcctccgct tagataccag aggtctggtg
    11641 ttagttggat aatcttacca tttttagcag caggtgtttc agcgataagg gcattttcta
    11701 ggacaccgtc gttgctagag ttgtccccac cgatggcaag ggtacggttg atgacaaaga
    11761 ggatgtcagg gttgatttct ttgacacttt caaagctgac ttcttgtccg tggcgtgagt
    11821 cttcaaatgt tgtatcagtt ggtttgaatt tcaaggtttg gtacaagaaa gagaaacgag
    11881 atttggcacc aaaggctgcc atttttcctt cattaaggag gatcgcaagg gcctttttgt
    11941 cagagctttc atttttagta gcgacttctt ggatgctctt gtctagcttg gccaattctt
    12001 ccttggcttt ctgtgtacca gtttcgccga aggcacttgc taaggattcg atattagcct
    12061 tggtagaagt ccagtagtcg tccttgcttg cttggaagag aacggttggg gcgatttctt
    12121 tgaatttgtc tacgaatttt tgtgtacgtg gtgaagcgat aatcaaatca ggctcaaggg
    12181 cggcgatagc ttctaaatca ggttctttca tagaaccaac atttttgaca gttcccacta
    12241 ggtcttttag ataagtcgga acagtttttg taggcattcc gacgatattt ttttcaaatc
    12301 ctaaagcgcg aatagtatcc gcagcgccga ggtcaaaggt cacaatcttt tcaggaactt
    12361 tggaaagttt gacctcgtcc agtgaacttt taatggttac ctctgttgga gcagagctac
    12421 tggtctctgt ctgactagtg cttgagtttg tactacatgc accaagtagg agcaagaagc
    12481 tggccactag ggcagtgaaa taaagtttaa gggatgtttt cataatttct cctttttaaa
    12541 atgtgataac gatttaggga gtctcttgat cttattgatt aagagactga aggttctcta
    12601 acttgagctt ttatgttact agctatagat acagatcttt ttgtcattga tatcagctag
    12661 cgtgatggga atctcataaa gttggctgag caggtcagcc tgcatgattt gatcggtgct
    12721 tcccttgcta aagacctgac cgtccttgaa ggcgacaatt tcatctgcat actgactggc
    12781 catgttgata tcgtggagga cgatgataat ggtcttgccg agttcctcca ccagtcgtcg
    12841 aagaatttgc atcatgctga cgctttgctt gatatcgaga ttgttgagtg gttcgtccag
    12901 caagataaag tccgtatcct gggccagtac catagcgata aagacgcgct ggagttgccc
    12961 ccctgacagg ctattgatgt agcggtcttt taagttggtc agttccaaat agttcagagt
    13021 ttctcggatt ttttcccagt cttctgatct aagtcgacct cggctgtagg gaaaacgtcc
    13081 aaaactgacc agttcttcaa cagtcaattt ggcttggtaa ttgattttct gttttaggat
    13141 ggttagttct tgggccagtt cttgcgaatt ccagctctcg atttcacgtc ctttgatact
    13201 gagaactccc tgatctttct tggttagtct gctcatgatg gagaggagag tcgattttcc
    13261 agcaccattt ggaccaataa aggctgtcag tttttgagga ctgacttcaa gcgaaatgcc
    13321 ttgcaaaata tcctgttttt gaatggattt gtcaatgttt tccagtttca ctgacgagcc
    13381 ctcctatata gtaagataaa gaataagaag ccacccacac tctcaatgat catactgata
    13441 cgaatttcca gtgcaaagac tcgttcaatc aaggcttgcc ccaaggttaa gctaataaat
    13501 ccaaccagaa tggccactat aaagagtaac ttgtgctgat agtctttgac aatcaggtag
    13561 gtgaggttgg ccagcataaa gccgaagaag gccataggtc ctaccaaggc agtggccgtt
    13621 gaggtcaaaa gcacgattcc ccagaggagc tctttctgtt ctttttcaac atcgagtccc
    13681 aatatctgag ccgtttctct ttgcaggtgc aagacatcta gaacgactgc ttttcgaaag
    13741 aaaaagattg tcaaagcgag gatgatcaga gaaccgatgg ctaggatgga agtgttaaga
    13801 tgttgaaagg aggcaaaaag actattttgc agtttatcgt attcgtttgg atccattagg
    13861 acttgaagga aggtgctgat atttcgaaag agacttctga gcgctagaca gatcagcagg
    13921 atgaagacca ggtcttgctt catcagtgtc ttcaagtaac cttgtaaggc gagaaagaag
    13981 agggactgga caagaagtaa gactaggaat tctaagatag gggatttgcc aagttgaaga
    14041 aacttgcttt caaaaaccag tagtagggtt tgtagtagga cgtagaagga ttcaattccc
    14101 aaaatactag gcgtcaggaa gcgattttcc gtcaaggttt gaaaactaat ggtcgaaatc
    14161 ccagtcgcga tggctaccaa gagataaacg atgatctttt gggaacgcaa cttccaagca
    14221 aaggctgaca agtgagtgat gggccaaaag tagagaagac aagctccgat ggcaagaata
    14281 atgagaatcc agaagagctt ggtatgtttg cttttagtct gcatcttttc gtccccctct
    14341 ccagagaagt aggataaaga cgagactacc gatgattcct agcaagagac tgacagacaa
    14401 ctcatagggc ctaatcagaa ctcgggatag gatatcgcaa gccagaacta gattggcacc
    14461 aaccagtgcg accatgagtt tggtttgact tagattatct ccatagcact tgcgaacaag
    14521 attgggaacg ataactccga gaaatggtag gccacccacg gtaatcatgg tgacgcttgt
    14581 cgttagcgcc accagaaaga gggccagttt ttcaagtagg gagtaggaaa tccccaaact
    14641 ctcgctggtt tctttcccta gattcatgat ggtgaaggtt tgggataatt tccaaacggt
    14701 tatcaggatg atgaggccta agaagagcca ctcatactga tgggtctgaa tcatggagaa
    14761 ggagccctgg gtccaggcag tcatactctg aaccagattg aaacgatagg cgataacttc
    14821 tgtgactgag ccgataatcc cgctatagat gatcccaatc agaggcaaca tccacctttc
    14881 ctttacagta aaaatggtca taaaggctag gaagaagagg gtgaatacga tggatgaaac
    14941 aaaagcgaag agcatctttt gggtcagact agccgatgga aagacaaaaa ggctcagcac
    15001 cattcccagt ttggcggctt cagtcgttcc aactgtactc ggtgcagcaa actgattttg
    15061 ggtaatagtc tgcatgagaa ggcctgccat actcatacta gaggcagtca ggagaatact
    15121 gatagttctt gggagacggg actcttgaaa gaggagccag gtctgctggt cgaaatcaaa
    15181 tagctttccc catgaaaaat cactggtccc aatgctaata gagagaaaga ctaggagtag
    15241 aagtaagcca attaaataat gagaaagttt cataccccgt cctttcatgt agatttggta
    15301 tcgaaagata tctgcggata taaatgtaac attatttttc taatctgtca ataaaatttc
    15361 tgacaattta ataaatacaa caaggagaga gcaacaagac tttctccttt gttatcctat
    15421 tctaaaatgt ttttacctta atctgataaa ataatatctt cgagggagta gctagccgtc
    15481 caatcaagat attgtttagc ttttgaagca tctgctagga cactggctgg gtcactagca
    15541 cgtcgagcaa caatctcgtg tgggattttt taatttagta attcttcagc agttttaaag
    15601 atttctttga tagtatagcc ttttttagtt cctaagttaa agatttgaga agaactgtct
    15661 tcttgaaata ggtagttcat tcctttaaca tgagcctatg caaggtccaa gacataaatg
    15721 taatctcgaa tacatgaacc gtcacgtgta tcgtagtcat ctccaaatat ttttaagcta
    15781 tcattttgtc ccaatgcggt cttgttgata tttggaatga tgtgagttgg atttttcaca
    15841 cgcagaccgt ttgaagcatc catttcagcc ccagcaacat taaagtaacg gaaaataaca
    15901 tatttccagt cgtagcgatt ggccatccag taaatcattc gttcgcccat cagttttgtc
    15961 tctgtataag ggttgacagg gtcgatcagg gtatcttcag tcgccggctt gtcaatacag
    16021 ttatttccat agagagaagc agtcgaagag aacatgattt tttgagtgcc aacttcagat
    16081 aagactttga gaacttggtt cctaccagca acgttggcag tgaagtattt acttggattt
    16141 tcaatacttt cgctcacaac aatttcacct gcataatgga ggacagcatc aatctgattt
    16201 tcttttaagt aagcctttaa ggcgctagca tcataaacat ccagttcttt aaagctagca
    16261 cgactatcca cagccgctcg atttcctgtg gagagattat cgaggacatg caccttatag
    16321 ccagcattta aaagagcttt aacggtatgg gagccaatgt agctagtccc acctgtaaca
    16381 agaattgttt tggtcatgat tttttccttt tctagttttg tactatagta
//
LOCUS       DAVLTM010000038        16257 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLTM010000038 DAVLTM010000000
VERSION     DAVLTM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.038"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            365..1642
                     /locus_tag="VYN16_001042"
     CDS             365..1642
                     /locus_tag="VYN16_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1403589.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1718..3202
                     /locus_tag="VYN16_001043"
     CDS             1718..3202
                     /locus_tag="VYN16_001043"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1403590.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALCEKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPVRHKTTVAAADMQAAVEAYLGL"
     gene            3263..4543
                     /locus_tag="VYN16_001044"
     CDS             3263..4543
                     /locus_tag="VYN16_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1403591.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYEKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4547..5167
                     /locus_tag="VYN16_001045"
     CDS             4547..5167
                     /locus_tag="VYN16_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1403592.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5333..6475
                     /locus_tag="VYN16_001046"
     CDS             5333..6475
                     /locus_tag="VYN16_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1403593.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVAERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6881..7219
                     /locus_tag="VYN16_001047"
     CDS             6881..7219
                     /locus_tag="VYN16_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1403594.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7230..7588
                     /locus_tag="VYN16_001048"
                     /pseudo
     CDS             7230..7588
                     /locus_tag="VYN16_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            7695..8312
                     /gene="pcp"
                     /locus_tag="VYN16_001049"
                     /pseudo
     CDS             7695..8312
                     /gene="pcp"
                     /locus_tag="VYN16_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8340..9197)
                     /locus_tag="VYN16_001050"
     CDS             complement(8340..9197)
                     /locus_tag="VYN16_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1403595.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9327..10469
                     /gene="tgt"
                     /locus_tag="VYN16_001051"
     CDS             9327..10469
                     /gene="tgt"
                     /locus_tag="VYN16_001051"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1403596.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10567..12384
                     /locus_tag="VYN16_001052"
     CDS             10567..12384
                     /locus_tag="VYN16_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1403597.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGIVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12537..13688
                     /gene="nagA"
                     /locus_tag="VYN16_001053"
     CDS             12537..13688
                     /gene="nagA"
                     /locus_tag="VYN16_001053"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1403598.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13851..14909
                     /locus_tag="VYN16_001054"
     CDS             13851..14909
                     /locus_tag="VYN16_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1403599.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(14958..16213)
                     /locus_tag="VYN16_001055"
     CDS             complement(join(14958..14995,14995..16213))
                     /locus_tag="VYN16_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1403600.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGL
                     IEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRN
                     FENFKKRIFIALNIKKERTNFVLSRA"
BASE COUNT         4786 a         2833 c         3716 g         4922 t
ORIGIN      
        1 aagaattgtt ttcagcttat gatgaagaag taagaaaatg caaagtattt ttcgaagaaa
       61 agagaacagt atttaaatat agtgaataag tcaggaatac taaagtgtta ctaaaaataa
      121 aaaaactatc gtttttgtga tagaggtaac tttttgaaaa ggctatttga ctggctttat
      181 atacttatga aatgttaaga gcccaactca aaatccagtg tccgcaagga cgtgccggtt
      241 cgaccccggc cgccggtata gtatagtgtt aggaacgttg ttattcttcg ttcctttttt
      301 atattatttt tggtataatt atagttattc aaattttatt tagattaaga aagtgtaggg
      361 gagtatgtct tgttctatcg atttattaaa acatcggtat ttgaaaaata ttaaagaaaa
      421 tcctgaattg tttgtcggaa ttgagttgga gtatcctgtt gcaagtttag aaggggatgc
      481 tacagatgtt gaagttataa aggatctatt tcattattta gtttctactt tggatctcac
      541 cgtagcaaag gtagatgatt ttggcaatct gatccagtta gtagatccga taagtcagga
      601 tgctatttta tttgaagttt cctatacaac gattgagttt gcatttggta aggctgaaac
      661 gattcaagag gtcgaaaatc gtttcaataa ttatatgaat gtaattcaga gaaagttagc
      721 tgaatcaaat catgctattg ttggctgtgg tatccatccc aactgggata aaaatgagaa
      781 ttgtccagtg gcttatccac gctatcagat gttgatggat tatttgaatt tgagtagaaa
      841 tattattaaa tcagatttac atcatttccc tgaatatggt acttttatct gtgggagcca
      901 ggttcagctg gatatttcaa aaaccaacta cttacgggtg attaatgctt ttactcaaat
      961 tgaagcggct aaggcttatt tatttgcaaa ctctgagttt tcgggtgcgg attgggatac
     1021 gaaaatttca agggatattt tctgggaaga atctatgcat ggtatctatc cagagaatgt
     1081 tggggtcaat gctagactcc ttaatgatga agctgatttt tttgactatc taaatcattc
     1141 tgcgattttt actgcggaac gtgatgggca gacctattat ttttatccta ttcaggctgg
     1201 ggactatttg gctacgcctg aaatccaagc atttgctctg aatggggatg aggttattat
     1261 ttccccccaa gagaaggatt ttgaaactca tcgtagttac cagtaccaag acttaacgac
     1321 tcgaggaaca gttgagtttc gtagtgtgtg tacacagcca cttgatagga cttttgcttc
     1381 tgcagctttt cacttgggat tattggttaa tttagacaag ttagaagctt acttagaaac
     1441 agcacctttc tttaaagtat ttggttatga ttacaagttt ttaaggagac aattttctaa
     1501 gaaaaatctt acagatgagg aagaaactac gattattgaa ttttccaaag acttactcct
     1561 actagctgag gagggactag tggtgagaaa taaggaagaa atgacctatt tacagccttt
     1621 gagagaagaa ttgagcctat aatttctctt ataaagggag aattttctga aaaatcatga
     1681 tataatggac gagactatag ataaaggata gagagtaatg acattagttt atcaatcaac
     1741 gcgtgatgcc aacaatacag taactgccag ccaagcaatt ttgcaaggtt tggcgacgga
     1801 cggcggtttg tttacaccgg ttacttatcc aaaggtagat ttggactttg acaaattgaa
     1861 agatgcttct tatcaggaag ttgctaagct agttttgtca gcatttttag atgactttac
     1921 agctgaggag ttggactact gtatcaacaa tgcctacgat agcaaatttg atactccagc
     1981 tattgcacca ttagtgaaat tagatgggca atacaatttg gaacttttcc atggttcaac
     2041 gattgccttt aaggatatgg ccttgtctat tttgccatac tttatgacga ctgctgctaa
     2101 gaaacatggt ttggagaaca agattgttat cttgacagcg acatctggtg acacggggaa
     2161 agctgctatg gcggggtttg cggatgtgcc tggtactgag attatcgtct tttatccaaa
     2221 ggatggtgtc agcaagattc aagagttaca aatgaccact caaactggcg acaatactca
     2281 tgttattgct attgatggta actttgacga tgcgcaaaca aatgtgaagc acatgtttaa
     2341 cgacgtggct ctttgtgaaa aattgactac caacaagttg caattttcat cagctaactc
     2401 tatgaacatt ggtcgtctgg tgccacagat tgtttattat gtttatgcct atgctcagtt
     2461 ggtcaagact ggtgagatcg tggctggtga taagattaac ttcacagtac caacaggaaa
     2521 ctttggaaat atcttggctg ccttttatgc caaacaaatt ggtttgccag ttggtaaatt
     2581 aatctgtgct tcaaatgaca ataatgtctt gacagacttc tttaaaacac gtgtctatga
     2641 caaaaaacgt gagtttaagg taacaaccag cccatctatg gatatcttgg tatcttcaaa
     2701 cttggagcgc ttgattttcc atcttttggg aaataatgct gaaaagacag ctgaacttat
     2761 gaatgccttg aacacgcaag gacaatataa gttgacagac tttgatgcag agattttgga
     2821 cctctttgca gctgaatatg cgactgagga agaaacggca gcagagatca agcgtgtttg
     2881 tgagttagat tcttatatcg aggaccctca tacagctgtt gcttcagcag tttataaaaa
     2941 ataccaatcg gccactggag atgtaactaa gacagtgatt gcttcaacag ctagtccata
     3001 caagttccca gtagttgcag tagaagctgt aactggaaaa gcaggtttaa cagactttga
     3061 agccttggct caattacatg aaatctcagg cgttgcagtg ccaccagcag ttgatgggct
     3121 tgaaatagct cctgttcgtc acaagacaac tgtagcggct gctgatatgc aagcagcggt
     3181 tgaggcttat ttaggacttt aagatagagg gagtaaactc ggttgggaaa ccaactgagt
     3241 ttcttttcat caggaggaga gattgtttaa gaaaaataaa gacattctta atattgcatt
     3301 gccagctatg ggtgaaaact ttttgcagat gctaatggga atggtggaca gttatttggt
     3361 tgctcattta ggattgatag ctatttcagg ggtttcagta gctggtaata ttatcaccat
     3421 ttatcaggcg attttcatcg ctctgggagc tgctatttcc agtgttattt caaaaagcat
     3481 agggcagaaa gaccagtcga agttggccta tcatgtgact gaggcgttga agattacctt
     3541 actattaagt ttccttttag gatttttgtc catcttcgct gggaaagaga tgataggact
     3601 tttggggacg gagagggatg tagctgagag tggtggactg tatctatctt tggtaggcgg
     3661 atcgattgtt ctcttaggtt taatgactag tctgggagcc ttgattcgtg caacgcataa
     3721 tccacgtctg cctctctatg ttagtttttt atccaatgcc ttgaatattc ttttttcaag
     3781 tctagctatt tttgttctgg atatggggat agctggtgtt gcttggggga caattgtgtc
     3841 tcgtttggtt ggtcttgtga ttttgtggtc acaattaaaa ctgccttatg agaagccaac
     3901 ttttggttta gataaggaac tgttgacctt ggctttacca gcagctggag agcgacttat
     3961 gatgagggct ggagatgtag tgatcattgc cttggtcgtt tcttttggga cggaggcagt
     4021 tgctgggaat gcaatcggag aagtcttgac ccagtttaac tatatgcctg cctttggcgt
     4081 cgctacggca acggtcatgc tgttggcccg agcagttgga gaggatgatt ggaaaagagt
     4141 tgctagtttg agtaaacaaa ccttttggct ttctctgttc ctcatgttgc ccctgtcctt
     4201 tagtatatat gtcttgggtg taccattaac tcatctctat acgactgatt ctctagcggt
     4261 ggaggctagt gttctagtga cactgttttc actacttggg acccctatga cgacaggaac
     4321 agtcatctat acggcagtct ggcagggatt aggaaatgca cgcctccctt tttatgcgac
     4381 aagtataggc atgtggtgta tccgcattgg gacaggatat ctgatgggga ttgtgcttgg
     4441 ttggggcttg cctggtattt gggcagggtc tctcttggat aatggttttc gctggttatt
     4501 tctacgctat cgttaccagc gctatatgag cttgaaagga taggaaatgc aaaaaacagc
     4561 ttttatttgg gatttagacg ggactttatt ggactcttac gaagcgattt tatcagggat
     4621 tgaggagact tttgctcagt tttctattcc ttatgataag gagaaggtga gagagtttat
     4681 cttcaagtat tcggtgcaaa atttgcttgt gcgggtggca gaagatagaa atctggatgt
     4741 tgaggtgcta aatcaggtgc gtgcccagag tctggctgag aagaatgctc aggtagtttt
     4801 gatgccaggt gcgcgtgagg tgctagcttg ggcagacgaa tcaggaattc agcagtttat
     4861 atatactcat aaggggaaca acgcttttac cattctcaag gacttggggg tggaatccta
     4921 ttttacagag attttaacca gtcagagtgg ctttgtgcgg aagccaagtc cagaagcggc
     4981 tacctatctg ctagataagt atcagttgaa ttctgataat acttattata taggggatcg
     5041 gactctggat gtggaatttg cccagaatag tgggattcaa agtatcaact ttttagagtc
     5101 tacttatgaa gggaatcaca ggattcaagc gttagcagat atttcccgta tttttgagac
     5161 taagtgataa aaagattgtg tcagttttgt gacagagacc taacaaacta tttcaagtaa
     5221 cctagtttgt tacaaggagt agacagttct gttaaatagg cccgagaggg ctttttttct
     5281 acattttttg tgttatgata gacaggtact catttgaaag gaatttgaaa gaatgaagaa
     5341 aagaatgtta ttagcgtcaa cagtagcctt gtcatttgcc ccagtattgg caactcaagc
     5401 agaagaagtt ctttggactg cacgtagtgt tgagcaaatc caaaacgatt tgactaaaac
     5461 ggacaacaaa acaagttata ccgtacagta tggtgatact ttgagcacca ttgcagaagc
     5521 cttgggtgta gatgtcacag tgcttgcgaa tctgaacaaa atcactaata tggacttgat
     5581 tttcccagaa actgttttga caacgactgt caatgaagca gaagaagtaa cagaagttga
     5641 aatccaaaca cctcaagcag actctagtga agaagtgaca actgcgacag cagatttgac
     5701 cactaatcaa gtgaccgttg atgatcaaac tgttcaggtt gcagaccttt ctcaaccgat
     5761 tgcagaagct ccaaaagaag tagcatcaag ttcagaagtt acaaagacag tgattgcttc
     5821 tgaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacgg ccgaaacaac
     5881 tcgcccagtt gaagaagcaa ctcctcagga aacgactcca gctgagaagc aggaaacaca
     5941 agcaagccct caagctgcat cagcagtgga agcaactaca acaagttcag aagcaaaaga
     6001 agtagcatca tcaaatggag ctacagcagc agtttctact tatcaaccag aagaaacgaa
     6061 agtaatttca acaacttacg aggcaccagc tgcgcccgat tatgctggac ttgcagtagc
     6121 aaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc tttaaagaag aaattgctaa
     6181 cttgtttggc attacatcct ttagtggtta tcgtccagga gacagtggag atcacggaaa
     6241 aggtttggct atcgacttta tggtagcaga acgttcagaa ttaggggata agattgcgga
     6301 atatgctatt caaaacatgg ccagccgtgg cattagttac atcatctgga aacaacgttt
     6361 ctatgctcca ttcgatagca aatatgggcc agctaacact tggaacccaa tgccagaccg
     6421 tggtagtgtg acagaaaatc actatgatca cgttcacgtt tcaatgaatg gataaacccg
     6481 acttgataac atcattttga cgaatgagat ctagctttcg tgatggaaag cgattctcgt
     6541 tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatatgaa
     6601 acctcaaagc tgtgctttga gcaacctgcg actagcttcc tagtttgctc tttgattttc
     6661 attgagtatc aatttgaatg gaaaatggaa agttattatc ttgtaataat ccaagcaaca
     6721 ttcttgcaat ctattttact ttatatcaca attgattggt caactattga taagtcaatg
     6781 gataggagga agaaatgata gagattcaag atttactgta tcaactccgc ttgtctgagc
     6841 aagcgagtac gcaattgttt gaaaaaggct tgggattagt ttgacacggt atcagatttt
     6901 actgtttttg ctggagcatt ctccttgtaa ccaaatggcg gttcaggagc gtttgaaaat
     6961 tgatcaggct gctttgacac ggcatttcaa gattttggaa acggaaggtt tggtggagcg
     7021 tcatcgtaat cctgaaaatc agcgggaagt gttggtagag gctgcgaagt atgccaagga
     7081 gcagttagtg gtgaatcccc ctctgcaaca tatcaaggtt aaggaagaga tggaaagtat
     7141 cttaacagag tttgagagaa cagaactcag ccgtttatta aataaattgg ttttgggtat
     7201 tgaaaatata gaaatttaag gagaaataga tgtcaattat tttaacaacg atcgttgctt
     7261 tggagcattt ttatattttt tatttggaaa gtattgccac gcaatcagat gcgactagtc
     7321 gtgtatttaa tatggaaaag gaagaattgg ctcatccgtc agtaagttca ttgttcaaaa
     7381 atcaaggaat ttataaggct ctgctaggag tctttctctt gtatgtcatt tatttctcac
     7441 agaatttaga aattgtgact atttttgtct tatttgtgat tggtgctgcg acttacggct
     7501 ctttaacagc ggataaaaaa ttattttgaa gcaaggtgga tcagctattt tggccttgat
     7561 tagtatttta ctctttaaat acacttgaag gttgattcta atctcgctaa tcctttttaa
     7621 tccagaataa gggaaatatg ttatacttgt ttttaagaaa aaagtctcat tgaattggtt
     7681 ttgaggagtt agaaatgaaa gtattagtga caggttttga gcccttttga ggccattaaa
     7741 ggtttaccag ctgaaatcca tggtgctgag gtccgttagc tagaggtgcc gacagttttt
     7801 cacaaatctg ctcaagtatt ggaagaagag atgaatcgtt atcaacctga ctttgtcctt
     7861 tgtattgggc aagctggtgg aagaactagt ttgacacctg aacgagtggc cattaatcaa
     7921 gacgatgcac gcatttctga taacgaagat aatcaaccga ttgaccgtcc cattcgccca
     7981 gatggtgctt cggcctactt tagtagtttg ccgattaaag cgatggttca agctataaaa
     8041 aagagggctt accggcctct gtttccaata cggcagggac ttttgtctgc agccatttga
     8101 tgtatcaggc tctctatttg gtagaaaaga aatttccata tgttaaggca ggttttatgc
     8161 atattcctta tatgatggaa caggtggtga acagacagac tattccagct atgagtttag
     8221 tggatattcg gcgagggata gaagcagcaa tcggcgctat gatagaacat ggagatcagg
     8281 aactcaagtt ggtaggcgga gaaactcatt gatagaaaaa agcttgagtg gaaaaacctt
     8341 caagcttttg gacgttttcg agccaatact gctcggtaaa acataatttt agtgcattgg
     8401 atataaggta ggagtgaaaa actagcaatg ccaaaggtaa tccaattgag gaagtaccaa
     8461 ggaagaagct gtaaatctag gacaaagcgc tggaacttgt agcccttcat aaaggaacgg
     8521 ctagttttta ggattcgtct tggtgggacc tgtcctaggt ctagactata acagagaaga
     8581 aattccacct gtgaataggc ataatactgt ggaatataga ggatatttcc tacaatgatc
     8641 aagatgagac ttgcaagaaa gtagagtcca aagaccatga ggaaacgctc ggtttcaact
     8701 gatgagagat ctagatttgg aaactcagga tgtagggtga cgaatttttt ggctaaaaag
     8761 ctactataaa agaggaggta aatcccaagt aaattaggga tactccataa aaagagatag
     8821 aaacgtttga gaagtagggt caaaaaggtt tgagaaaagc gctcctcatc aaagagagct
     8881 aggctgtttt ttacagatgg ctccgtttta gaatctttca tgagtgtcag tgttgcatag
     8941 acggaactgg tcaaaagaat agtcccgata aaggagacta gtagaggaaa gaggtaggtt
     9001 tgaagtattt ggccaagtat gctgaaaaat ggctgttcta aaacagtccc gtggatccga
     9061 gataagggat taagaaaact agataagatg accagcatac tgggaaggat atagaggaga
     9121 aagagacggg gggtgtcagc ctgaaaatgt tttgactcct gacgaattgt ttttaaatca
     9181 atttttggat agttcattct cttattatac catagttctt atacatagtt cgtgacagtt
     9241 cctacttttt ttgataaaat catacagtgt gtccttgggc acactgtatg aactgggact
     9301 gtctttccca gcttcggagg taaaaaatgt cagattcacc aatcaaatat cgtttgatta
     9361 agaaagaaaa acacacagga gctcgtctgg gagaaatcat cactccccac ggtacctttc
     9421 cgacacctat gtttatgcca gttgggacac aagccactgt caaaactcag tcacctgaag
     9481 aattgaagga gatgggttcg ggaattatcc tatcaaacac ctatcatctc tggcttcgcc
     9541 ctggagatga actcattgca cgcgctggtg gtctccacaa gttcatgaat tgggaccagc
     9601 ctatcttgac agatagtggt ggttttcagg tttattcttt agcagatagc cgtaatatca
     9661 cagaagaagg agtaaccttt aaaaatcatc taaatggttc taagatgttc ctatccccag
     9721 aaaaagccat ctctattcag aataatctgg gttcagacat catgatgtcc tttgatgaat
     9781 gtcctcagtt ttatcaacct tatgactacg ttaagaaatc gatcgagcgt accagccgtt
     9841 gggctgagcg tggtttgaag gctcaccgtc gtccacatga ccaaggtttg tttggaattg
     9901 tgcaaggtgc aggatttgaa gaccttcgcc gccaatcagc tcatgatctt gtcagcatgg
     9961 atttctcagg ctactctatc ggtggtttgg cagtgggaga aacccatgaa gagatgaatg
    10021 cggtcttgga ctttacaact caactgctgc ctgaaaataa acctcgttat ctgatgggtg
    10081 tgggagcgcc agatagcttg atcgatgggg tcattcgtgg ggtggatatg tttgactgtg
    10141 tcttaccgac tcgaattgct cgtaacggga cttgtatgac cagtcaagga cgtttggttg
    10201 tgaaaaatgc ccagtttgct gaggacttta cgccactgga tcctgagtgt gactgctaca
    10261 catgtaataa ctatacacgc gcttaccttc gtcacctgct caaggctgat gaaacctttg
    10321 gtatccgctt gactagctac cacaatcttt acttcttgct taacctgatg aagcaagtgc
    10381 gacaagccat catggatgac aatctcttgg aattccgtga gtattttgtg gaaaaatatg
    10441 gctataataa gtcaggacgt aatttctaaa atggaattga tataaaaaaa tcctaagttt
    10501 tctcttagga tttttcttct ttttttgata gaataaagtg tacaatgaaa ggaagaataa
    10561 actcgtatgc gcattaaatg gttttccttg gttaggatta caggtttact tttggtactc
    10621 ttgtatcact tctttcagac gatctttcct ggaggatttt tcggggtaga tgtctttttc
    10681 acattttcag gtttcctgat tacagctcta ctcattgaag aattttctaa aaaccatgag
    10741 attgatttga taggattttt tagaagacgc ttttatcgga ttgtgccacc tgtggttttg
    10801 atggtcttgg tgaccatgcc ttttactttc ttggttcgcc aagactatgt tgctggaatt
    10861 ggtggccaga ttgcgagcgt cttaggcttt atgaccaact tctatgaact cctaacaggt
    10921 gggagttatg aatctcagtt cattcctcat ttgtttgttc ataattggag tttggcagtt
    10981 gaggttcact actatattct ttggggattg gcagtttggt tcttatccaa acaagctaaa
    11041 tcaaatggtc agttgaaggg gatagtcttt ctcttatctg ctgttgcctt cttgatcagt
    11101 ttcttctcca tgtttattgg tagttttcta gtgacctctt attcctctgt ttatttctcc
    11161 agtttaactc atgtctatcc attctttttg ggaagtatgt tagcaactat tgtaggtgtt
    11221 cgtcagacga cttccctcgt caagcagttg gataaaatct gggatttacg aaagactttg
    11281 gtagtttttg gaggaggctt tggtttccta gttcttttga ctttctttgt caaattcact
    11341 tatctttttg cctatcttat cggcttcttg cttgccagtc ttgcagctct tgccatgatt
    11401 ctggcggcgc gtgtcttaca tgaaaagaca catcatatac aggagccgaa gattatcagc
    11461 tttttagcgg atactagcta tgcggtttat cttttccatt ggcctttcta tatcattttc
    11521 tcacagttga catcaaatct tcttgctgta ttactgactc tgatttgttc ttatggcttt
    11581 gccagtctgt cattctatgt attggaacct tggattgcag gcaagaacac acctatttta
    11641 caaactcttc gtcccctgcc ttatattcac gcaattcttg tagcaggtac aggaatcttg
    11701 accatcattg tctgcacggt gaccttgttg gcaccacaag tgggagcgtt tgagacagac
    11761 ttgactgtca atggcttgaa gcaagctgca acaaatattg gccagaccaa ggtgatggca
    11821 gaacgggcag atgcaaacag tttgggaatt gctgatggca ctatgttaat tggtgactca
    11881 gtggctttaa gggcaaatac agcactacag acagctcttc ctggagcaca gattaacgcg
    11941 caggtcagcg taacaaccaa gaccgcaaat gaaatcatgc taaataatag ccagaataaa
    12001 tttttaccta agacggtggt cattgcgact ggggtaaata atcctgagaa ttacaaggat
    12061 gactgggaca gtatcgtgaa aaatcttcct aagggacacc atatgatttt ggtgactcct
    12121 tatgagggag ataagacaaa agagacctat gccatcgttg agaaggctgc tgcctatatg
    12181 agagaattgg cagagaagac accttacatt acgatagcag attggaatca agttgcgaaa
    12241 gagcatccag aaatttgggc aggaacagac caggttcatt tcgggagtga gagtagcact
    12301 atcgaagcag gagcaaaatt gtatgcagat acgattgcca cagctttgca gacagctcaa
    12361 gacaagccgg ttaaatcaaa ataacttgta ttaaaaaaga gaagagttgg agaaatccag
    12421 ctcttttaaa atatctccaa aaaataggtc tataccattt acaaataaaa aagaaaggtt
    12481 tataatgtaa ttgacataat aaattgcaga atcaatcttt taaggaggtt aacattatgc
    12541 ctaactatat taaagcggat cagtttttct acccacacgg agttcgtcga ggtggttact
    12601 tggaacttgt ggacggcaag tttgggaaac atgtagagca gattcctgaa ggggctgagg
    12661 tgattgacta tacaggttat agcattgccc caggtcttgt ggatactcat attcatggat
    12721 atgcaggtgt agatgtgatg gacaacaaca ttgaaggtac attgcatact atgagtgaag
    12781 gacttcttag taccggtgtt accagtttct tacccacaac tttaacagcc acttatgagc
    12841 aattgcttgc agtcactgaa aatcttggaa accattataa agaagcaaca ggtgctaaga
    12901 ttcgtgggat ttattatgaa ggtccatatt tcacagaaac ttttaagggg gcacaaaatc
    12961 caacttatat gagagacccg ggtgttgagg agtttcattc ttggcaaaaa gcggcaaatg
    13021 gcttgcttaa taaaattgcc cttgcaccag aacgtgatgg ggtggaagac tttgttcgta
    13081 cagttacggg cgaaggtgtg acggttgctc ttggacattc aaacgcgact tttgatgaag
    13141 ccaaaaaagc agtcgatgct ggagcgagtg tttgggtgca tgcctacaat ggaatgcgtg
    13201 ggttgactca ccgtgaattg ggtatggttg gagccatgta ccaattgcca catacctatg
    13261 cagagttgat ctgtgatggt caccacgtag atccaaaggc ttgcgaaatt cttatcaaac
    13321 aaaaaggaac agaaaacatc gctcttatca cagactgtat gacagctggg ggattggaag
    13381 acggagatta tatgttggga gaattcccag ttgtcgttgc aaatggaact gcacgcctca
    13441 aatcgacagg taacttggca ggttctatcc tcaaactcaa agatggtttg aagaatgtgg
    13501 tcgaatgggg aattgcgaat ccgcatgaag cagtcatgat ggccagcttc aacccagcta
    13561 aatccgttca catcgatgac gtctgtggcc aaatccgtga aggctacgac gctgacttca
    13621 tcgtattaga taaagatttg gaattggtag caacctacct agatggcgta aaacgttatc
    13681 aagcataaga gagaaagcag aagttagaga ctaacttctg cttttttatg cagggtactt
    13741 tactggtaaa taaaaagtta aaaaaatcac aaaaaaagct tgaagaaaca aagaaaaagg
    13801 cttacaattg aactataaat attacaaata aaaaaacgga ggagtgcttt atgaaagcct
    13861 atacttatgt taaaccagga cttgcttctt ttgttgatgt agacaaacca gttattcgca
    13921 agccaacaga cgctattgtg cgtattgtaa aaaccactat ttgtggaaca gacctccata
    13981 ttatcaaagg ggatgttcct acttgccaaa gtggtaccat tcttggccac gaagggattg
    14041 ggattgttga agaagttggg gaaggagttt ccaacttcaa aaaaggtgac aaggtcttga
    14101 tttcttgcgt ctgtgcctgt ggtaaatgct actactgtaa aaaaggaatt tatgctcact
    14161 gtgaagacga agggggctgg attttcggtc acttgattga tggtatgcag gctgaatatc
    14221 tacgtgtccc tcatgcagat aatactcttt accatactcc agaagacttg tcagatgaag
    14281 ctttggttat gctgtcagac attctgccta ctggatatga aattggtgtc ttaaaaggga
    14341 aagtagaacc tggttgcagc gtagccatta ttggttcagg tccagttgga ttggctgctc
    14401 ttttaacagc ccaattctat tcaccagcta aattgattat ggtagaccta gacgataacc
    14461 gcttggaaac tgccctatca ttcggtgcga ctcataaggt taattcttca gaccctgaaa
    14521 aagccattaa agaaatttat gatttgacag atggtcgtgg tgtggatgtc gctatcgaag
    14581 ctgttggtat tcctgcaaca tttgatttct gtcaaaagat tatcggtgta gacggaacgg
    14641 ttgccaactg tggtgtgcat ggtaaaccag ttgaattcga tttagataaa ctttggattc
    14701 gcaacatcaa tgtaacaact ggtttggtat ctacaaatac gactccacaa ttgttgaaag
    14761 cgcttgaaag tcataagatt gaaccagaaa aattggtaac tcactatttc aaactcagtg
    14821 aaattgaaaa agcctacgaa gtcttcagta aggcagcaga ccaccatgcc attaaggtca
    14881 ttatcgaaaa cgatatctca gaagcctaag tagtaaaaat atttggctct ttgtcaactg
    14941 tagtgggttg aagtcagcta agctcgagaa aggacaaaat tcgtcctttc tttttgatgt
    15001 tcagagcgat aaaaatccgt tttttgaagt tttcaaagtt ccgaaaacca aaggcattgc
    15061 gcttgataag tttgatgaga ttattggtcg cttccaattt ggcgttagaa tagtgtagtt
    15121 gaagggcgtt gacgattttc tctttgtcct ttagaaaggt tttaaagaca gtctgaaaaa
    15181 gaggatgaac ctgcttaaga ttgtcctcaa tgagtccgaa aaatttctcc ggttccttat
    15241 tctgaaagtg aaaaagtaag agttgataga gctgatagtg gtgtttcaag tcttctgaat
    15301 agcttaaaat cttgtcaaga atttctttat ttgttaagtg catgcgaaaa gtagggcgat
    15361 aaaaacgttt atcgctcaat ttacgactat cctgttggat gagtttccag taacgtttga
    15421 tagccttgta ttcatgagat tttcgttcaa actgattcat aatttgaaca cgaaaacgac
    15481 tcatggcacg gctgagatgt tggataatat ggaaacgatc tagaacgatt ttagcacacg
    15541 gaaaaagctg tttagccaag tcatagtaag gactaaacat atccatcgta atgattttca
    15601 cttgacaacg aacggctcta tcgtagcgaa gaaagtgatt tcggatgaca gcttgtgttc
    15661 tgccttcaag aacagtgata atattaagat tatcaaaatc ttgcgcaatg aaactcatct
    15721 ttcccttagt gaaggcatac tcatcccaag acataatctt tggaagccga gaaaaatcat
    15781 gctcaaagtg aaagtcattg agcttgcgaa tgacagttga agttgaaatg gccagctgat
    15841 gggcaatatc agtcatagaa attttttcaa ttaacttttg agcaattttt tggttgatga
    15901 tacgagggat ttggtgattt ttctttacca ggggagtctc agcaaccatc atttttgaac
    15961 agtgatagca cttgaaacgg cgttttctaa ggagaattct agaaggcata ccagttgttt
    16021 cgaggtaagg gatcttagac ggtttttgaa agtcatattt cttcattaga cttccacaat
    16081 cagggcaaga tggagcctca taatccagct tagcgataat ttctttgtgg gtatccatat
    16141 tgatgatatc tagaatcttg atgtttgggt ctttaatatc gagcagtttt gtgataaaat
    16201 gtaattgttc catatgaatc tttctaatga tggttttgtc gcttttcatt ataggtc
//
LOCUS       DAVLTM010000039        16033 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLTM010000039 DAVLTM010000000
VERSION     DAVLTM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.039"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            108..2273
                     /locus_tag="VYN16_001056"
     CDS             108..2273
                     /locus_tag="VYN16_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1403601.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2273..3013
                     /locus_tag="VYN16_001057"
     CDS             2273..3013
                     /locus_tag="VYN16_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1403602.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3162..4649
                     /locus_tag="VYN16_001058"
     CDS             3162..4649
                     /locus_tag="VYN16_001058"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1403603.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(4695..5972)
                     /gene="ftsY"
                     /locus_tag="VYN16_001059"
     CDS             complement(4695..5972)
                     /gene="ftsY"
                     /locus_tag="VYN16_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1403604.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5976..6794)
                     /locus_tag="VYN16_001060"
     CDS             complement(5976..6794)
                     /locus_tag="VYN16_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1403605.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKVAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6794..7588)
                     /locus_tag="VYN16_001061"
     CDS             complement(6794..7588)
                     /locus_tag="VYN16_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1403606.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7585..11124)
                     /gene="smc"
                     /locus_tag="VYN16_001062"
     CDS             complement(7585..11124)
                     /gene="smc"
                     /locus_tag="VYN16_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1403607.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAVLEDKRNS
                     LSQEKSDKESSLVLLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAASAGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARKVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELDNLNLAEQEVQDLEK
                     AIRSLGPVNLDAIDQYEEVHSRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11115..11813)
                     /locus_tag="VYN16_001063"
     CDS             complement(11115..11813)
                     /locus_tag="VYN16_001063"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1403608.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(11996..12067)
                     /locus_tag="VYN16_001064"
     tRNA            complement(11996..12067)
                     /locus_tag="VYN16_001064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12032..12034),aa:Arg,seq:ccg)
     gene            complement(12131..13117)
                     /locus_tag="VYN16_001065"
     CDS             complement(12131..13117)
                     /locus_tag="VYN16_001065"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1403609.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13292..14608)
                     /locus_tag="VYN16_001066"
     CDS             complement(13292..14608)
                     /locus_tag="VYN16_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1403610.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNPLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIENQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14595..>16033)
                     /locus_tag="VYN16_001067"
     CDS             complement(14595..>16033)
                     /locus_tag="VYN16_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1403611.1"
                     /translation="TEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKT
                     YPELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGT
                     GKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIF
                     KDFCQKAKEPQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYD
                     SGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDK
                     KFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYI
                     IGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAI
                     ENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKK
                     AFDLTNNEQKDQAVADDNEGDENDDADY"
BASE COUNT         4627 a         3565 c         2875 g         4966 t
ORIGIN      
        1 gaaaaggaaa aatcaatgtt atattttctg acacgaaaaa tataaaaatt tgcctttttt
       61 tactttttct gatataatga gagaatattc ggaaaaggag actaaaaatg aagaaaaaat
      121 ttctagcatt tttgctaatt ttattcccaa ttttctcatt aggtattgcc aaagcagaaa
      181 cgattaagat tgtttctgat accgcctatg caccttttga gtttaaagat tcagatcaaa
      241 cttataaagg aattgatgtt gacattatta acaaagtcgc tgagattaaa ggctggaaca
      301 ttcagatgtc ctatcctgga tttgacgcag cagtcaatgc ggttcaagct gggcaagccg
      361 acgctatcat ggcagggatg acaaagacta aagaacgtga aaaagtcttc accatgtctg
      421 atacttacta tgatacaaaa gttgtcattg ctactacaaa gtcacacaaa attagcaagt
      481 acgaccaatt aactggcaaa accgttggtg ttaaaaacgg aactgccgct caacgtttcc
      541 ttgaaacaat caaagataaa tacggcttta ctattaaaac atttgacact ggtgatttaa
      601 tgaacaacag cttgagtgct ggtgccatcg atgccatgat ggatgacaaa cctgttatcg
      661 aatatgccat taaccaaggt caagacctcc atattgaaat ggatggtgaa gctgtaggaa
      721 gttttgcttt cggtgtgaaa aaaggaagta aatacgagca cctagttact gaatttaacc
      781 aagccttgtc tgaaatgaaa aaagatggta gtcttgataa aattatcaag aaatggactg
      841 cttcatcatc ttcagcagtg ccaactacaa ctactctcgc aggattaaaa gctattcctg
      901 ttaaggctaa atatatcatt gccagcgatt cttcttttgc accttttgtt ttccaaaatt
      961 caagcaacca atacactggt attgatatgg aattgattaa ggcaatcgct aaagaccaag
     1021 gttttgaaat tgaaatcacc aaccctggtt ttgatgctgc tatcagtgct gtccaagctg
     1081 gtcaagccga tggtatcatc gctggtatgt ctgtcacaga tgctcgtaag gcaacttttg
     1141 acttctcaga atcatactac actgctaata ccattcttgg tgtcaaagaa tcaagcaata
     1201 ttgcttctta tgaagatcta aaaggaaaga cagtcggtgt taaaaacgga actgcttctc
     1261 aaaccttcct aacagaaaat caaagcaaat acggctacaa aatcaaaacc tttgctgatg
     1321 gttcttcaat gtatgacagt ttaaacactg gtgccattga tgccgttatg gatgatgaac
     1381 ctgttctcaa atattctatc agccaaggtc aaaaattgaa aactccaatc tctggaactc
     1441 caatcggtga aacagccttt gccgttaaaa aaggagcaaa tccagaactg attgaaatgt
     1501 tcaacaacgg acttgcaaac cttaaagcaa acggtgaatt ccaaaagatt cttgacaaat
     1561 acctagctag cgaatcttca actgcttcaa caagtactgt tgacgaaaca acgctctggg
     1621 gcttgcttca aaacaactac aaacaactcc ttagcggtct tggtatcact cttgctctag
     1681 ctcttatctc atttgctatt gccattgtca tcggaattat cttcggtatg tttagcgtta
     1741 gcccatacaa atctcttcgc gtcatctctg agattttcgt tgacgttatt cgtggtattc
     1801 cattgatgat tcttgcagcc ttcatcttct ggggaattcc aaacttcatc gagtctatca
     1861 caggccaaca aagcccaatt aacgactttg tagctggaac cattgccctc tcactcaatg
     1921 cggctgctta tatcgctgaa atcgttcgtg gtggtattca ggccgttcca gttggccaaa
     1981 tggaagccag ccgaagcttg ggtatctctt atggaaaaac catgcgtaag attatcttgc
     2041 cacaagcaac taaattgatg ttgccaaact ttgtcaacca attcgttatc gctcttaaag
     2101 atacaactat cgtatctgct atcggtttgg ttgaactctt ccaaactggt aagattatca
     2161 ttgctcgtaa ctaccaaagt ttcaagatgt atgcaatcct tgctatcttc tatcttgtaa
     2221 ttatcacact tttgactaga ctagcgaaac gcttagaaaa gaggattcgt taatggcaaa
     2281 attaaaaatt gatgtaaatg atttacacaa gcactatgga aaaaatgaag tcctaaaagg
     2341 aattacgact aagttctatg aaggagatgt tgtttgtatc atcggtcctt caggttctgg
     2401 taagtcaact ttcctccgta gcctcaatct tttagaagaa gtcactagcg gtcacatcac
     2461 tgtgaacggc tatgatttaa ctgaaaaaac aaccaatgtt gaccacgtcc gtgaaaatat
     2521 cggcatggta ttccaacact tcaacctctt ccctcatatg tctgtattgg acaacatcac
     2581 ctttgctcct attgagcaca agttgatgac taaggaagaa gctgagaaat tgggaatgga
     2641 gttgcttgaa aaggttggac tagcagataa agctaatgcc aatccagata gcctatcagg
     2701 tggtcaaaaa caacgtgtgg ccatagctcg tggcctagca atgaatccag acatcatgct
     2761 cttcgatgaa ccaacttctg cccttgaccc tgagatggtt ggagacgtac ttaacgttat
     2821 gaaggaattg gctgagcaag gcatgaccat gattatcgta acccatgaga tgggatttgc
     2881 tcgtcaggtt gccaaccgcg ttatctttac tgcagatggc gagttccttg aagacggaac
     2941 acctgaccaa atctttgata acccacaaca ccctcgtctg aaagagttct tagataaggt
     3001 cttaaacgtc taaactcaaa ctgtaaggat ttccttgcag tttttctacc tcgtattgga
     3061 atttttgatt tttcggaaaa ttatgttaga attaagttta tgaaatgagg tttcctcata
     3121 cctagcaaga ctaggaataa aaatagaaat taggtagcta gatgtcatct aaggttattg
     3181 ttacaatttt cggtgcgagt ggagacctgg ctaaacgcaa gctctaccct tcccttttta
     3241 gactatataa atccggcaat ctttccaagc actttgccgt tattggaact gcccgtagac
     3301 cttggagtaa ggaatatttt gaatctgtag ttgtcgagtc catccttgat ttggcagata
     3361 gtaccgagca agcccaagaa tttgctagcc acttctacta tcaaagccat gatgtcaatg
     3421 attcggaaca ttatattgct ttgcgtcaat tacaagctga gcttaatgaa aaataccaag
     3481 ctgaacacaa taagctcttc ttcttgtcta tggcacctca gttctttgga accattgcca
     3541 aacacctcaa atctgaaaac attgtcgatg gcaaaggttt tgagcgcttg atcgttgaaa
     3601 aaccatttgg tacagattac gcaactgcaa gcaagttgaa tgacgaactc ctagcaacat
     3661 ttgacgaaga acaaattttc cgtatcgacc attatcttgg taaggaaatg atccaaagca
     3721 tctttgcagt tcgctttgca aacttgattt ttgaaaacgt ttggaacaag gattttatcg
     3781 acaatgttca aattaccttt gcggagcgct tgggtgtaga agaacgtggt ggctactatg
     3841 accaatccgg tgccctccgt gacatggtcc aaaaccacac tctacaactt ctttcgctcc
     3901 tcgccatgga caaaccagca agcttcacaa aagacgagat tcgtgctgaa aagattaagg
     3961 tctttaaaaa cctctatcat ccaactgatg aagaactcaa agaacacttt atacgtgggc
     4021 aataccgctc tggtaagatt gatggcatga aatacatctc ttatcgtagc gagccaaatg
     4081 tgaatccaga atcaacaact gaaaccttta catctggtgc cttctttgta gacagcgatc
     4141 gattccgtag tgttcctttc tttttccgta caggtaaacg actgactgaa aaaggaaccc
     4201 atgtcaacat cgtctttaaa caaatggatt ctatctttgg agaaccactt gctccaaata
     4261 ttttgaccat ctatattcaa ccaacagaag gcttctctct tagcctaaat gggaagcaag
     4321 taggagaaga atttaacttg gctcctaact cacttgatta ccgtacagat gcgactgcaa
     4381 ctggtgcttc tccagaacca tacgaaaaat tgatttatga tgtcctaaat aacaactcaa
     4441 ctaactttag ccactgggat gaagtttgtg cgtcatggaa gttgattgac cgtattgaaa
     4501 agctctgggc tgaaaatggt gccccacttc atgactataa agctggaagc atgggacctc
     4561 aagccagctt tgacctactt gaaaaattcg gtgccaaatg gacttggcaa ccagatatca
     4621 cctatcgtca agatggtcgc ttagaataaa aaaatttcct gcaagtttat gccttgcagg
     4681 atttttgctt ctgattagat taaaccttcc aagagacctt tcataaagtt ttctgagtta
     4741 aactctccaa tatcatcgat tttttcacca aaaccaatca attttacagg aatattgagt
     4801 tcttcacgaa tggctagaac cacacctcct cgagcagttc catcaatctt agtcaaaaca
     4861 attcccgtta aaggtgtgat tttcgaaaat tctttggcct gtactagggc attttgacct
     4921 gttgatgcat caagtgccaa gaaggtttca tgtggtgctt ctggcacaac acgtttgata
     4981 atacgaccaa tcttttccaa ctcagccata aggttatcct tattttgcag acgaccagca
     5041 gtatcaatca tgagaatatc gataccttca gccacggcac gttccatacc atcaaagacc
     5101 acgctggctg gatcagcttt ttcaggtcca gttactactg gaacatctac tcgtcggccc
     5161 cattcagcta gctgagctac tgcacccgca cggaaggtat ctgctgcaac cagcatgacc
     5221 ttcttaccag cttgtttgta gcggtgggct agttttccga tagaagttgt tttcccaaca
     5281 ccattcacac caacaaagag cataactgtc aagttatctt ggaagtggat gctttcatcg
     5341 tagctaccat ccttttcata aagctcaacc aatttctcaa tgatgacacg acgaagtgca
     5401 tcaggtttct tggcattttc aagcttggct tcgtaacgta gttcctccgt taagttagaa
     5461 gcgacttgga caccaacatc actcataatc aacagttctt ccagttcctc gaaaaattct
     5521 tcgtcaacag agcggaagtt agcaaagaag gcattcaagc gggcaccgaa acctgtgcga
     5581 gttttcttaa gactgcggtc atacttttcc tgaacagttt cttctgtttg aggcctttct
     5641 gcttcaagaa tttcagaatt attttcttct acagtctcta aaaactcagt attcccttct
     5701 tctgagtgtg gaaattcttc gacttctttt tgagaaactt ctacaactgg ctctaaatcc
     5761 agactttctt caactgtgtc ttggatttcc tcttcttgga acacagcttg ttcaacaatt
     5821 tcaacctctg cttcttcctg agaaacttcc tcaacttctg tgaaggtagg atcaacatct
     5881 tcagacaaat caagattttc cagagcttct ttcacaactt cttcgatttt aggttcttct
     5941 ttttttccga atagacggtc aaacaatccc atatcttagt tctcctttag cacatattct
     6001 tcgatagccc aggcgacagc ttcctcatcg ttggtcatcg gcgtcactac atttgcggct
     6061 acctttactt caggaacagc gttttgcata gcaacaccaa gacctgccca ttcaatcata
     6121 gagaggtcat tggcctcgtc accacaagcc atcacttgac tttggtcgat tccaagatgg
     6181 ctgattagtt ttgccaaacc tgttgcttta tgaacattct ttggtgacca ttctagcaac
     6241 atttcacgtg atttaaagat ttcatattgg tcaaacaatt ctggagaaat cttctgaatg
     6301 gctgcatcca agggttcttg agcaaaggca gtcacgcatt tgttgtaggt catttgacta
     6361 gataagtctt caaagtccac tggaacaaag gtcaaagctg gattgaattt ggcataaaga
     6421 ctttcttggt ccgattggat ttgataaact gttccttctg agatggcatc aagaggcagt
     6481 gataatttct ctgtttcttc atacaaacgt gccacatcat catatgaaaa gactgtttta
     6541 tcaaggattt ctcctgtatt tttctgaact aatccaccat taaaagtaat ggtatactca
     6601 tcttcctgac cgtcagtccc taactcatgg agaaagaaat ccatggcttt taagggacga
     6661 ccagttgtca atacgacctt gataccacga tcacgcgcag ctttcaaggt ttccttggta
     6721 cgatccgtca gccttttatc agtagtcagc aaggtcccgt ccaagtccaa tgcaatcaat
     6781 tttatatctg ccattataag ccctccatat aagctataac cgaccgttcc ttatggtgac
     6841 caatcacagt ctttgctaat tctaaaattt caggtcgtgc attttcagga gctacaggat
     6901 gtcccacaac ctgcatcata tgtaagtcat taagattgtc tccaaaagcc atgacctgat
     6961 ccattgtgat accaagtttt ttagctaatt caacaatagc tacaccctta tcgacatagt
     7021 ccagaacaat atcaatagat tcaaagccag ttgtcatggc cttaacacca gggacatgtt
     7081 cattaaccca agcttcacca gcttctagcg tttcttctgt gaagttggtt gtaaatttga
     7141 aaatgtcatc tgtgatatct tccaaactcg ctactttttg gatattttca ttataatgct
     7201 gactcacttt caaataggtc tcatcaaccg tatctagaac atatgaaccc ttcttacccg
     7261 tcaagagcag tttattgata tctacataag gtgacgtttt cagcttttca aaagttgcca
     7321 gataaaagtc acgagacata gtcgcttcat acaagtcctg accttgatac tctaccaaac
     7381 tgccattttc cgcgatgaaa ataatgtcat cacgaacacc agcaaataat ttttccagag
     7441 acagaaatcc ccgacccgaa gccaccgcaa agtaaatccc tttttccttg taggaagcca
     7501 agagagactt gagacgatcc atatcaaagc gtccattccc atctaggaag gttccgtcca
     7561 tatccgttgc tactagttta attgtcatcc ttcaatactt tctaaatctt ttaacttaac
     7621 tgaaacaatc tttgaaacac ccgattcttg catggtcact ccatagatgg aatcagccgc
     7681 tgccatggtt cccttacggt gggttacaac gataaactgg ctgtccttgt caaagcggtt
     7741 gaggtaatcc ccaaaacgtt taacattggc ttcatccagc gcagcttcca cctcatccaa
     7801 gatgacaaaa ggaatggtct tgacacgaat aatggagaaa agcaaggcaa gagccgatag
     7861 agctttttca ccaccactca tgaggttaag cgactggatt ttcttacctg gaggttgaac
     7921 agaaatctcc acaccagctg ttaaaaggtc gccctcagtc aatatcaagt ctgcctgacc
     7981 tccgccaaac atctgcttga aggtcacttt aaaggactca cggatagctt caaaggttga
     8041 tttaaagcgt tccttgacct catcattcat ctctgtaata gtctcaagaa gcagattttt
     8101 cgctgacaaa atgtcatctc gctggctatt gaggaaatcc agacggctgt gaacttcttc
     8161 gtactggtca atagcatcca agttgacagg acccagtgag cgaatagcct tctctaaatc
     8221 ctgcacctct tgctctgcca gattgagatt gtccaactca tgcgcttttt ctagtgcttc
     8281 agtatagcta atctggtact ggtctgttaa ttgactttgt agatggcgca agcgctcgct
     8341 gaccttttct ttcttggctt cagcacgtgt ttgcttgcga atccactcct cattctgctg
     8401 gcgagcctga tccaaatgac tagcaatatc atccagctga ccttctatat catccaactc
     8461 aaactgtttg cgaatcaaac cttgttggag gttcgttttc tgagttttgg attcttccgc
     8521 ctgttgactg agcaattctg tatcaacctt ctcaagattg tcaacctttt cttgaagaag
     8581 gcgctgaatt tcttcttgct caatatcaag attatctagt tccttaccta agcgttcaat
     8641 atcagtcact tcataacgtt tttgcccttg cagttctgtc ttaagtagac gagcttgagc
     8701 tagctcttcc tgcaagtttt gataacgttc ttggatggcg tttttattag acttaatctc
     8761 ttcaatctca gcttccagat tattcttttc actagtgatt gtagtaaggc gctcttggca
     8821 tttttcctta tccgcttgcc attctccctc agaaagacga tttaattcct cttcttggag
     8881 tttccaaaga gtttctagct cttcaacttg ctgactggtc tgctgatagg cgagggacaa
     8941 gccttgctcc tgaatacgag cctgctctcc ctgagatttg atggcttcta atctttctgt
     9001 caatctagct agctcatctt gcaagttctt caaactcact tcatctgaac gcaaacttgc
     9061 ttcttcttca gcaatttctt tttgtaattg ctccagttct ggcttgatga aaatactgtt
     9121 attctgacga ttggctccac ctgcgtaaga accacctgtg cgcaactctg taccgtccaa
     9181 agtcaccata cgaacctgat aacgaacttt gcgcgctgct gcacgcgcat gctcaacggt
     9241 atcaaaaatc gctgtcgtag ctagcaagtt cttgaaaata gcttccagtc tcgtatcgaa
     9301 tgtcaccaac tcatctgcca tgcctagaaa tcccgcactt gcagcgataa catcttggtt
     9361 ctgactagaa atcgtacgcg ccttgatagt cgtcaacgga aggaaggttg cacgaccggc
     9421 tctgttccgt ttaaggaagt caatagcctt ggttgccgac tcttcatctt ctacgatgat
     9481 atgctggcta cttgccccta aggcaatctc taaggcagtt tgataatgca catcaaaggt
     9541 caaatgctca ctaacagcac caataatccc accaaggcgg tctttttctt ggagaacact
     9601 ctttacacct gcataaaagt tactgtgatt tctaaggatg ttttccaaac tttgagctct
     9661 agcctgcttg tttttaagac tatccagacg gtcaaagagt tgactttgtt gagcttgata
     9721 ggaagttttt tgctcctctt gctccttggc aatagcttga tagtcagcca ataatttctg
     9781 aacctgcacc ttggcagttt caagctcgtc tttttgctga ctagccttct ctttagctgt
     9841 agctaattgc tctttcagct tttctagttg atctgcttgt ttttgagaaa gctgacgact
     9901 attttccaac tcattctcga tgcgggtcag ttggtttgag acatccgctt cttcttgtaa
     9961 aagagctaca aagcgttcac gtaagagctc aatcatctga tcaggatcat ctgaaaaagc
    10021 tagcaattca gcttctaaac gattgagttt ttgattattt tggactagat tttcctctaa
    10081 aagtactaaa gagctttctt tatcagattt ttcttggctg agtgaatttc tcttatcctc
    10141 caaaacagcc aaacgagctt gtgcttcctg ttgattcagg gctacttgct cagattctaa
    10201 tttcgatagg gctaattttc tctctaaatc actaatcaga ctggtcaaat ccatcaaact
    10261 gccttggtct ttggccattt cagcctgtaa atcttggcgt tgctttttaa gagtttgatt
    10321 ttcttcttct aatttttcac gcttttggta ataactcatc aagagttctt gaacctgagc
    10381 caactcttct tctgtcgact ctagttcagc cttattttcc ttgatttgag caaccagaac
    10441 atctaaataa atagccttac gttgtccttc caagtctaaa aacttacggg cattctcagc
    10501 ttgcttttca agaggcttga tttgattatc caactcgtag ataatgtcct ctaagcggtc
    10561 cagattatcc tgagtttgct gcagtttact ctcggtttct tttctgcgag tcttgtattt
    10621 taaaactcca gcagcttctt caaaaatagc tcgtcgttcc tcaggcttgg aattaaaaat
    10681 ctcctcaacc ttcccttggg aaataataga gaaggaatct cgtcccaatc cagtatccaa
    10741 gaagaggtca tgaatatcac gcagacggac tttcttgccg tcaatcttgt attcgctatc
    10801 tccactacga tagatatggc gttccaccct gatttcttga cctgcatcct tgataaatcc
    10861 gtcatgatta tccagagtca caactacaga agcataattg agcggtttgc gactttcggt
    10921 tccagcaaag atgacatccg gcatcttgcc cccacggaga ctcttgacac tagactcccc
    10981 caaagcccaa cgcagacttt ctgtaatatt ggactttcca gatccattgg gtccaacaac
    11041 tgccgtcaca ccttggtcaa aaacgacctt ggtcttatca gcaaaagact tgaacccctg
    11101 aatttcgatt tcctttaaat acatgaatcc agccccttct caacggcatt tttggcagct
    11161 tcctgctctg ctaatttctt agaacgacct tggccttgac cgatgctctt accttcaaca
    11221 agaacttcta catcaaagac cttatcatga gcaggccctg tttcagaaat cacctgataa
    11281 cgaatagcca catcaccatt gacctgaagc aactcttgga gatgggtttt atagtctgta
    11341 atcatctcaa actcgcctgc ttcaacctta ggaatgatga cttgatagat aaattccttg
    11401 accttggcca catccttatc caaaagaagg gcaccaagaa aggcttcaaa ggcatcacca
    11461 agaatggtgt cacgattgcg accacctgat ttttcttccc ctttacccaa cttgataaac
    11521 tggttaaact ggcaatcacg cgcaaaacca gctaaactct cctcacggac aatcatagca
    11581 cggagttttg ataggtcacc ttcaggcttt ttaggatatt ttttatatag atattctgaa
    11641 atcaataact gtagaacagc gtctcctaaa aattccaagc gttcattgtg tgaaattttt
    11701 aagaggcggt gctcattggc ataactcgta tgagtaaagg cagtttccag taaatttttg
    11761 tctgcaaatt cgattgcaaa atgattcttt agtacagttt gtaattcttt cataccaacc
    11821 tctttctaac tgataatagt cctttttatt atatcaaaaa aagccccctg agtcactcta
    11881 aaacgggact ggaaagcatt tgggaattct ttagacagag attctcagtt ttagcggcaa
    11941 atttgggtca ggataaagaa aaaagcccta ttaaaggctt tttaggatgt ttacatccac
    12001 cctgagggaa tcgaaccccc atctcaagaa ccggaatctt acgtgatatc cattacacta
    12061 agggtggaaa cttgttttat tataacagaa atttgctcta ataacaagtt ttttggtcaa
    12121 agaccccgtc ttagtgggaa gcatccccat tccagatgga gtttttcacg atcacataat
    12181 caacgtgttt aaggtcagca acctgacgtc cacctgcata agaaatagca ctttgaaggt
    12241 cttgttccat ctcagttaaa gtgtcttgca gatgaccttt agcaggaagc aagatacgtt
    12301 tgccttccac atttttgtaa gcaccttttt gatattgtga ggctgaacca taatattctt
    12361 tgaactgttc accatcgact tcaatcgttt tccctggact ttcaatgtgt cctgcaaaga
    12421 gggaaccaat catgaccatg ctagcaccga agcggataga cttggcaata tcaccgtgag
    12481 tacgaattcc tccatcagcg ataatcggtt tacgcgcagc cttggcacac cagcgtagag
    12541 cagccaactg ccaaccacct gtaccaaaac cagtcttaac cttggtgata caaaccttac
    12601 caggaccgat tccgacctta gtagcatccg caccagcatt ttccaattca cgcacagctt
    12661 ctggtgttcc cacatttcca gcaattacaa aggtatctgg caattctttc ttgatgtgtt
    12721 gaatcataga aatcacgcta tccgcatgac catgagcaat atcaatagtg atatactcag
    12781 gagcatcagt cttgagctgt ctaacgaaat catactcata gtccttaaca ccgacagata
    12841 tggaagcaat gagcccttga tcgtgcattc gtttaataaa aggaatgcgt cctgcctcat
    12901 caaaacggtg cataatgtag aagtaaccac ctttagccag ttgctctgct acattttcat
    12961 ccaaaatcgt ctgcatattc gctggcacaa caggtagttt aaaggtgtga tttcctaaag
    13021 tgacacttgt atccgcttct gcacggcttt taatgacaca tttatttgga atcaattgaa
    13081 tatcttcgta atcaaaaatt ggaaattcat ttaacatatc gatgtctcgt ttcttttgta
    13141 atgacctacc tatgctctcg catcactacg ccttttccga cgtttcctta atttattata
    13201 aaccaaagta cagtttttgt caaattattt tatgaattaa aatatatcgt tcgggttttg
    13261 cttgtgttaa aaataaaaga aagaggggaa attattttct ccccacttct tcatcaataa
    13321 tcgctttaaa attttcttct gaattttcca tcattttaca aaatgcacta taggatgagg
    13381 cattctgagg gattcgaata gaccacttct tcaattgagc tccatagcca gctacttttc
    13441 ctatttcact atttactgac tgctccatac taggaaaaat cagtccagcc ttctcagctt
    13501 ttaaaatata agaataggaa atcagctgga ataagtcctc acggttgatt cctttttcag
    13561 tcaattccag ttttttatat tttgcatcta gaacaatctt tcgttctctg tcataaaaat
    13621 ctggatatac ttttcgtttc ccaacagaaa atactgaaat tccatccgtc ttatccttat
    13681 ttctgggatg tacaaaacct tttggcaaca aggtgtaaac atactcttcc caaagccagg
    13741 caacatcaaa gagaatacca tagatttttt gatcttgata ccctaaaccg tgcttttctt
    13801 ggtttaggat catcagacaa agttcttgta agtttctgta ctcgtgaaag tatgcatgac
    13861 gtataggttt agattgattt ccccgaataa tcttagcacg atcagctagt ttataagagg
    13921 gcgttacacg cacgatttca gatacgtttt cacgactagt tgagagatta tctagtaccc
    13981 cttgaccaat gcttttctga ttctcaatgt attcaatagt gtgacggacc aactgcatga
    14041 ggggattatc ataggtgaac tctctcgttg tgtaggcaat atttcccgtg aaaggaagat
    14101 ttttcttgag atggcttctt acatcaatca ctcccttaac atgactgtcg ttatgagaaa
    14161 atcgatgata ttccttataa agaccttttc gaatagcagc ttgtagatac ttggggaaga
    14221 gatacatcaa aagttgataa agcctctctt cacgagacaa agcaacatct aaactagtga
    14281 gattgatatg aagaaccttg tttaagagat aatgcaaaaa atggtcatta ctctcatcag
    14341 aaaatcgtga ggaaatcgtt aatctttcct gaccatatcc aagaaaacca atcacgttcc
    14401 ctgtcttaat tttctgattg actgtttcaa aaatcttttg gtccttatcc aaatcaggag
    14461 tatgagtcaa atcatttgga aaaatgaaaa tacgttcatc ttgggataga ctttccaagg
    14521 ttctgtctaa aagaggattg cttagtttag gatattcttc aacaaatttt tctttaataa
    14581 tcttgtgttg attatcagta atccgcatca tcgttttcat cgccttcatt gtcatcagct
    14641 actgcctgat ctttttgctc attatttgtc agatcaaatg cttttttcaa agtttccaaa
    14701 gtttcaacct catcataaga acctcgcaag tagtcttcta ggagaggctt aatataatca
    14761 gaccagagta attcatagtc aaaatctaca tccttcaact taagaaaata acttggtcca
    14821 atatgataat gactgtttaa ttcctgaaca ttttcgatag cagcgttcaa gtttcttaga
    14881 cgaatttttg cttcttctgc atggatattc aactctttat ccaacatgcc aacttgaccc
    14941 tcggcagtaa cttcaacaaa acgaaaacga cgacgcatag caaaatcaaa ggtatccact
    15001 gaacgatcaa tatcattcat agttccgatg atgtaaacat tttcggggat atagaacttt
    15061 tcatcagttt cgtgtagatt tgcatattgg gtagaaacac ttcctttttc accacgatag
    15121 ccagggtcga tagagaaaaa gagttcgcca aaaatcttag aaatctcccc acgattgatt
    15181 tcatcgatga tgaagacgaa tttcttatca gtatcaattt ctgttgggga aacatagtct
    15241 ttcaaaccaa atctctttct caatgtttct aggacagttt ttccaccact tttgtagtat
    15301 tcttgcttat tataattttt atctttatac aactcgtaaa catatttgct aggtagtgac
    15361 catcctggaa caccactatc ataatttact gacaaatttt gtctactatt aacagataag
    15421 taagatgttt ttgttatata ttctttttct tcagcaacat ttatatattc taagtaagaa
    15481 tcccaagcct catcaaaatt atcttgtcct ccaatcaatt ggggttcttt tgctttctga
    15541 caaaaatctt taaaaatacc gtcctgtagc ctaaactcaa tagctccatc cccatttgat
    15601 actggtctta aaccttctac aaaatccgta taatcatagg atgggtgaaa ttgtacaaat
    15661 ccgatttggt cttcgttgcc atccgttaat tctttggcaa tttctttagc aagataagtc
    15721 tttcctgtgc caggagcacc acgaaggata aggtttggag actttaatag agattgagtt
    15781 aattgatgac taaaattgtt tgatgaatcc attgtactat tgtcttcctt attttccata
    15841 aaaagcttag aatactcaaa aagcatagtt cctaatttcg atggctccaa ctcaggataa
    15901 gttttggaca ccagttcaat tattttatga ttttgcagat aaggtccacc ttgatttgtc
    15961 tgagaaacaa agtggttcca aaattctttc tgactattaa tatttacacc aaagaaagga
    16021 tctccctcag tat
//
LOCUS       DAVLTM010000040        15887 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLTM010000040 DAVLTM010000000
VERSION     DAVLTM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.040"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            67..615
                     /locus_tag="VYN16_001068"
     CDS             67..615
                     /locus_tag="VYN16_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1403612.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            725..1624
                     /locus_tag="VYN16_001069"
     CDS             725..1624
                     /locus_tag="VYN16_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1403613.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDMLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPERRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1649..2923)
                     /gene="serS"
                     /locus_tag="VYN16_001070"
     CDS             complement(1649..2923)
                     /gene="serS"
                     /locus_tag="VYN16_001070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1403614.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3137..3508)
                     /locus_tag="VYN16_001071"
     CDS             complement(3137..3508)
                     /locus_tag="VYN16_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1403615.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3511..4875)
                     /locus_tag="VYN16_001072"
     CDS             complement(3511..4875)
                     /locus_tag="VYN16_001072"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1403616.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5200..5985
                     /locus_tag="VYN16_001073"
     CDS             5200..5985
                     /locus_tag="VYN16_001073"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1403617.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6079..6513
                     /locus_tag="VYN16_001074"
     CDS             6079..6513
                     /locus_tag="VYN16_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1403618.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6513..7487
                     /locus_tag="VYN16_001075"
     CDS             6513..7487
                     /locus_tag="VYN16_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1403619.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLISLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7547..7771
                     /locus_tag="VYN16_001076"
     CDS             7547..7771
                     /locus_tag="VYN16_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1403620.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7890..8864
                     /gene="fabK"
                     /locus_tag="VYN16_001077"
     CDS             7890..8864
                     /gene="fabK"
                     /locus_tag="VYN16_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1403621.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8857..9777
                     /gene="fabD"
                     /locus_tag="VYN16_001078"
     CDS             8857..9777
                     /gene="fabD"
                     /locus_tag="VYN16_001078"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1403622.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPVVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEATVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9811..10542
                     /gene="fabG"
                     /locus_tag="VYN16_001079"
     CDS             9811..10542
                     /gene="fabG"
                     /locus_tag="VYN16_001079"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1403623.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10564..11799
                     /gene="fabF"
                     /locus_tag="VYN16_001080"
     CDS             10564..11799
                     /gene="fabF"
                     /locus_tag="VYN16_001080"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1403624.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFIKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEYAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAVKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11802..12287
                     /locus_tag="VYN16_001081"
     CDS             11802..12287
                     /locus_tag="VYN16_001081"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1403625.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12284..12706
                     /gene="fabZ"
                     /locus_tag="VYN16_001082"
     CDS             12284..12706
                     /gene="fabZ"
                     /locus_tag="VYN16_001082"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1403626.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            12718..14085
                     /gene="accC"
                     /locus_tag="VYN16_001083"
     CDS             12718..14085
                     /gene="accC"
                     /locus_tag="VYN16_001083"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1403627.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14122..14988
                     /locus_tag="VYN16_001084"
     CDS             14122..14988
                     /locus_tag="VYN16_001084"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1403628.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            14985..15752
                     /locus_tag="VYN16_001085"
     CDS             14985..15752
                     /locus_tag="VYN16_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1403629.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
BASE COUNT         4538 a         3024 c         3724 g         4601 t
ORIGIN      
        1 taggaatcag gattttataa gattttccaa taatattagt gtcaataata agaaaagagg
       61 tatcttatga caacatttac tatccataca gtagagtcag caccagcaga agtgaaagaa
      121 attcttgaaa cagtagaaaa agacaacaat ggctatattc ccaacctaat cggtctcttg
      181 gccaatgccc cgactgtttt agaagcctac caaattgtct catctatcca ccgtcgcaac
      241 agcctgacac ccgttgagcg tgaagtggtg caaatcacgg cagccgtgac caatggttgt
      301 gccttctgtg tcgcaggtca cacagccttt tccatcaaac aaatccagat gaatgatgac
      361 ttgattcaag ctcttcgcaa tcgtactcca attgaaacag atcctaaatt ggatacccta
      421 gctaagttta ccttggcagt tatcaatacc aagggtcgtg taggagatga agccttgtct
      481 gagtttttag aagctggcta cactcaacaa aatgccttgg atgtggtttt cggtgtcagc
      541 ctagcaatcc tctgtaacta tgccaacaac ttagctaata caccaattaa tccagaattg
      601 caaccttatg cctaattcat atctgagtaa aatgaagtct gaaataatcg gacttcgttt
      661 ttttaaatcc tcatttaagc gcttttatgt tatactgaaa ctaacatgat tattggaggt
      721 taggatgaaa aaactcccct tagtattttc aggttgtttg ctaggtttgg caggagctgg
      781 aaatcttatt ttagatatgt tgccagttct atcccatctt tttagtctga ctggtttggt
      841 tttatggatt tactttctaa ttctgcatct ctttaattgg aaagaaacca agcaagaatt
      901 gaccaagccc cctcttttgt caggaatggc aacctttcct atggctggga tgattttatc
      961 gacctatgtc tttcgcgtat tctcttatct tcctttggta gcacaaggga tttggtggtt
     1021 ttcatttctc ttggatttga ccttgattgc tggttttacc atcaagtttg cttgtccaga
     1081 gcggagggtt catgccactc caagctggac ggttctctat gtggggatag cagtggctgc
     1141 cttgacctat cctctggtag gtattatcga aattgcctat gcgaccttga gttttggttt
     1201 tctcttgacc ttctatctct atccccttat ttatagcgat ttaaagaaac atccactccc
     1261 actagccttg cttggacaag aaggaatcta ctgtgctcct ttctctctac tcttggcttc
     1321 tctagttcga gtaggaggaa ccagcctgcc gacttgggtc ttgattgtca tgattttggc
     1381 ttctcaatcc ttctttttct ttgttttaac tcgtctgccc aacattttaa aacaaggttt
     1441 tcaaccagcc ttctcagccc tcaccttccc aaccattatc acagcgacct cgctcaagat
     1501 ggctcaggga attttgaaac ttccatttct ggattacctg gtattggctg aaaccattat
     1561 atgcctaact attttattct ttgtactagg tgcttatctg atttggttac gaaaaaaggt
     1621 ctagctagaa atagctaaac cttatttttt atggtttgat gacttcagct ccacccatgt
     1681 atggacgaag tgcttctggg atggtcacag aaccatcttc attttggtaa ttttcaagaa
     1741 ttgcagccac tgtacgtcca actgcaagtc cagaaccgtt caaggtatga aggagtttca
     1801 ccttgccatc tgcttcatca cggtaacgga tttgggcacg acgggcttgg aaatcttctg
     1861 tgtttgaaca gcttgagatt tcacggtaat tgttttgtgc tggaatccac acttccaagt
     1921 cgtaagtctt cgtagctgag aagcccatat ctccagtaga gagagcaacg acacggtatg
     1981 gaaggttgag tttttgaaga atgttttcag cgttggctgt cattttttcc aattcttcgt
     2041 aagattcttc tggtttggca aatttgacca tttcaacctt gtggaattgg tgcaaacgga
     2101 tcaagccacg cgtatcacga ccggcagaac cagcctcaga acggaatgac ggactcatgg
     2161 cagtgaagta gattggaaga tctttgccgt ctaagatttc atcacggtag tagtttgtca
     2221 gaggaacttc agcagttgga atcaagacaa agttggtatc gctgagttca aaagtatctt
     2281 ccttaaattt tggatactga ccagtaccaa acatagaatc atggttgact atgtaaggtg
     2341 tgatgacttc agtatagcct tcttttccat gttcatccaa cataaagttg tagatagcac
     2401 gttccaaacg agcaccgagg cctttataga agaggaagcg agcgcctgtt accttaccac
     2461 cgcgttccca gtcaaggata ccaaggtctt caccgagatc ccagtgagct ttaggttcga
     2521 agtcaaactc gcgtggagta ccccaacggc gaacttccac attgtcgtct tcgtcagccc
     2581 caacaggaac gctgtcagct gggatatttg gaagagtcgt tgtaaattct gtcaatttag
     2641 catcgatttc tgccaattca gcatccaagg ctttaacctc agcagataga ttttgcatgg
     2701 cagcaatctt gtcatctgta ttttccttgt tgcgcttagc ttgggcaatc tcagcagaaa
     2761 ctgtgttacg ttctgctttg agagtttcaa ccttgaccaa gatgttacga cgtttagcat
     2821 cgatttcttt catttcattc aagacagcag catctacacc acgtgtagct aatttttctg
     2881 cgacagcttc aaaatctgta cgaatacgtt tgatatctaa cataagaact cctttatgaa
     2941 aaaagcacgc ctgacaaaac gttggagtgg cagggccacg gttccatcca acttcacagg
     3001 tgtgcacttg attgtgtatg taattgttac taacggtaga atttcaccta tccctcctat
     3061 ctgctcgcag tacccgcaga ctttctgaaa gaagaagata acctacttat ccgttgctat
     3121 gattatacta aagtttctac ttttttgcaa atagattttt aaatttttgg ctaattgtct
     3181 gaatcagggt cggaagtttg acgaccttgt cattgcctag ttttttgcgt gcaattttga
     3241 gaatggcacc tgagtctttt gaagcaaaga ggaattttcc tttgtctgta aagacttcga
     3301 agtggcggct gattttgcgt ccagtgacat tggctccaat ctgattgata tggctccaag
     3361 gaatctggat aaattgttcg acattgacat ctgggtaaaa ttccaaagcc tgatctccga
     3421 caaggaattt cccaactttc ccagcgatag agaggtagga agtgcctgtc gtactgagga
     3481 gtactgtttt gttaagtgat tgagccatgc ttagtcttcc ttactttctc caaaaaaggc
     3541 attgtagagg gctttaattg ctgctttctc ttggtcttta ttgacaacaa acataataga
     3601 aacttcacta gaaccttgag acatcatctg gatgttgatt ttgttttcag atagagcgcg
     3661 tgtcgcagta gcagtcactc cgatatggct cttcattttt tcaccaacaa tcataatgat
     3721 agaaaggtcg tgttcgattt ctgcatgatc tactttagcc ttttgaacca actgacgcag
     3781 gatttcttct tccttgatgg gagttagttg gcgagaacgg agaatgatag aaagatcgtc
     3841 gatacctgtt ggcatatgtt cccaaccgat gttaagttct tccaggattt gcagaacctt
     3901 gcggccaaat ccaacctcac ggttcatgag gtatttcgac atgttaatgc tgacaaagcc
     3961 tgagtcacca gcaattccca caactggaaa ttcatcatta ctgtgtttta gaacgatacg
     4021 agtacctgga tggtcagggt tgttggtatt cttgataacc agaggaattt ttccacggta
     4081 ggcaggaaga agagcctcgt catgaaggac tgagaagcct gcataggcca actcgcgcat
     4141 ttcacggtag gtcaactcag gaatcgagtg tggttggtgg ataataccag ggtgggctgc
     4201 aaagatacca tcaacgtccg taaagttttc atagaggtca gctttgacac cagcagcaat
     4261 gatagaacct gtaatatcag aacctccacg tgagaaagta cagatttgat tttccttagt
     4321 gacaccaaag aaaccaggaa tgacaaggac ttcattggtg tttgtcaatt cttcaatctt
     4381 gtcataactt gatggaatga tgcgagcgtg accaggttca cttgtgacca caatcccagc
     4441 ttctctaggg tgcatatagc gtgcatcgat accattttgg ttaaagtagg cagcaatcaa
     4501 tttggcattg ttattttcac cggctgctag gaaagtatcg tagagaaatt cattttcttc
     4561 aataggaaga gtggccaagg cgtgaatgct tttagaaatt ttttctagca cagctggttt
     4621 tagtcccaat tcactaacca tagcagcata gcggtcgata atccagcttt ggctcttgct
     4681 aatatcgtta cccgcaacat agtcgcggta gtatttaatc agggcatccg taaccttagt
     4741 atcttcagca ttgcgtttac caggcgcaga aacgactaca aaacgacgct ctgaatcgct
     4801 tttgacgatg tttaaaactt tttctaattg actagcagag gcaagagaac tacctccaaa
     4861 tttaacaact ttcataagaa ctcctaaagt aagtatttta tacgattata gcagaaagaa
     4921 aggctttttt caatgaagaa aataagatgc tttctagcct aaagcgagcc tttcttatcg
     4981 gctgagacac tttcagtcga ttttttcttg ctttctgctc ctaaaaaaga tatactttga
     5041 cagtgaaata atttaacact caatgaaaat caaagagcaa actaggaagc tagccgaagt
     5101 cagctcaaaa tactgttttg aggttgcaga tggaagctga cgcggtttaa agagattttc
     5161 gaagagtata aactgcttat aaaataaaaa ggagccctga tggaacacat tatttatcag
     5221 cttgaagagg acttggcaat ccttaccttg aaccgtcctg aggtcgcaaa tggttttcat
     5281 attcccatgt gtgaggagat tttagaagct ctgactttgg cagaagaaaa tccagctgtg
     5341 cattttatct taatcaatgc caatggaaag gtcttctcag ttgggggaga tttggtagag
     5401 atgaagcggg cagtggatga ggatgatatt ccatcattga caaaaatcgc agagttggtc
     5461 aatacgattt cttataaaat caagcaaata gccaaacctg ttttaatgga agttgacggt
     5521 gctgttgcag gtgccgcagc gaatatggct gttgcggcag atttctgttt ggcgacggat
     5581 aaggctaagt ttatccaagc ctttgttggt gttggtttgg ctccagatgc agggggaatt
     5641 catctcttga gccgcagtat cggtgtgacg cgtgctgctc aattagctat gacaggagag
     5701 gctttaacgg cagaaaaagc cctagaatgg gggttggttt accgcgtttc tgaagctgaa
     5761 aaacttgaaa agacgagaga acagcttctt aaaaaattaa gacgtgcttc aagtaattcc
     5821 tatgctgcca ttaagaagtt ggtttgggag agccagttta aagactggca gggttacgct
     5881 actttagaac tgaacctaca gaaatcctta gctcaaacag aggatttcaa agaaggagtg
     5941 cgagctcatt cggagagaag aagacctaaa tttacaggaa aataaaaaat acttgcatca
     6001 ttctttgaat tttgatataa ttctttctgt caaatgtttt gattgtaaaa gttttttgaa
     6061 gaaggaggga tatgaaaaat ggactaccaa cgaatcaatg aatatttaac atctatattt
     6121 aacaatgtcc ttgtaattga ggaagtgaac ttgagaggta gtcgttttaa ggatatctcc
     6181 atcaaagaaa tgcatacgat tgatgtcatc ggtaaggctc cagatgtgac tccaagtcaa
     6241 gtgtcaaaag agttgatggt aactcttgga actgttacga caagtttgaa caatttagag
     6301 cgtaagggct acattgagcg agttcggtca gaacaggatc gtcgtgtggt gcatctgcat
     6361 ttgacaaaga agggtcgctt gattcataga ctgcataaac gcttccacaa ggccatggta
     6421 gaaaaaatta ttgatggcat gagcgaggaa gaaattgctg tcatgggtaa aggcttgact
     6481 aatctttacc aatttttgga ggatttgaaa taatggcttt tgcaaaaata agtcaggttg
     6541 ctcattatgt gccagagcaa gtggttacaa atcacgactt ggctcagatt atggatacca
     6601 atgatgagtg gatttcaagt cgaacgggaa tacgacaaag gcatatttca agaacagaat
     6661 ctaccagtga tttggctaca gaggttgcta agaaactgat ggcaaaagct ggaataacag
     6721 gaaaagaact ggattttatc atcctagcta ccattactcc agattcgatg atgccctcta
     6781 cagctgctcg tgttcaagct aatattggcg ctaataaagc ctttgctttt gacttaaccg
     6841 cggcttgcag tggatttgta tttgctcttt caactgctga aaagtttatc gcttctggtc
     6901 gctttcaaaa aggcttggtg attggtagtg aaaccctctc taaggcagtc gattggtcgg
     6961 atcgatcaac agctgtgttg tttggagatg gtgctggtgg tgtcttgtta gaagctagcg
     7021 agcaagagca tttcttagct gagagtctca atagcgatgg aagtcgcagc gagtgtttaa
     7081 cttatgggca ttcaggtttg cattctccat tttcagatca agaaagtgca gattcgtttt
     7141 tgaagatgga tggacgcaca gtctttgatt ttgccattcg agatgtagcc aagtctatca
     7201 agcagactat tgatgaatct cctatagagg tgacagactt ggattatctg ctacttcatc
     7261 aagccaatga ccgtattttg gataagatgg ctagaaaaat tggtgttgac cgagccaaac
     7321 ttccagccaa tatgatggaa tatggcaata ccagtgcagc cagtatcccg attttacttt
     7381 cagagtgtgt agaacaaggt ctcatctctt tagatggtag ccagactgtt cttctatcag
     7441 gcttcggtgg aggcttgacc tggggcacgc tcattcttac aatttaggta atcatgtggt
     7501 gaacacattg ttataaaaaa actatttatt ttaaaggagt cctatcatgg cagtatttga
     7561 aaaagtacaa gaaattatcg ttgaagaact tggaaaagac gcatcagaag taacacttga
     7621 atcaactttt gatgatttgg acgcagattc attggacttg ttccaagtaa tctcagaaat
     7681 cgaagatgct tttgatatcc aaatcgaagc agaaaatgac ttgaaaacag ttggtgactt
     7741 ggttgcttac gttgaagagc aagcaaaata agcagaatat tagtagaagg agtagggaga
     7801 aacccactcc ctcttgttta ggtaatagtt tgactgtcaa attatggtga aatcgaacta
     7861 ttacgtaagc aaggaaggtt ggaggcacta tgaaaacgcg tattacagaa ttattgaaga
     7921 ttgactatcc tattttccaa ggagggatgg cctgggttgc tgatggtgat ttggcagggg
     7981 ctgtttccaa ggctggagga ttaggaatta tcggtggggg aaatgccccg aaagaagttg
     8041 tcaaggccaa tattgataaa atcaaatcat tgactgataa accctttggg gtcaacatca
     8101 tgctcttatc tccctttgtg gaagacatcg tggatctcgt tattgaagaa ggtgttaaag
     8161 ttgtcacaac aggagcagga aatccaagca agtatatgga acgtttccat gaagctggga
     8221 taatcgttat tcctgttgtt cctagtgtcg ctttagctaa acgcatggaa aaaatcggtg
     8281 cagacgctgt tattgcggaa ggaatggaag ctggggggca tatcggtaaa ttaacaacca
     8341 tgaccttggt gcgacaggta gccacagcta tatctattcc tgttattgct gcaggaggaa
     8401 ttgcggatgg tgaaggtgct gcggctggct ttatgctagg tgcagaggct gtacaggtgg
     8461 ggacacggtt tgtagttgca aaagagtcga atgctcatcc aaactacaag gagaaaattt
     8521 taaaagcaag ggatattgat actacgattt cagctcagca ctttggtcat gctgttcgtg
     8581 ctattaaaaa tcagttgact agagattttg aactggctga aaaagatgcc tttaagcagg
     8641 aagatcctga tttagaaatc tttgaacaaa tgggagcagg tgccctagcc aaagcagttg
     8701 ttcacggtga tgtggatggt ggctctgtca tggcaggtca aatcgcaggg cttgtttcta
     8761 aagaagaaac agctgaagaa atcctaaaag atttgtatta cggagccgct aagaaaattc
     8821 aagaagaagc ctctcgctgg gcaggagttg taagaaatga ctaaaacagc ctttttattt
     8881 gctggtcaag gtgcccagta tctagggatg ggacgggatt tctatgatca gtatccggtt
     8941 gtcaaagaaa cgattgatcg agcgagtcag gtgctcggtt atgatttacg ttatctcatc
     9001 gatacggaag aagacaaact caatcagacc cgctatacgc aaccagccat tctagcgact
     9061 tcggttgcta tctaccgttt attgcaagaa aagggctatc agcctgatat ggttgctggt
     9121 ttgtctcttg gagaatactc tgccttggtg gcaagcggcg ccttggattt tgaagatgcg
     9181 gttgccttgg tagctaagcg tggagcctat atggaagaag cggctcctgc tgactctggc
     9241 aagatggtag cagttctcaa tacgccagta gaggtcattg aagaagcctg tcaaaaagct
     9301 tctgaacttg gagtggttac tccagccaac tataacacac ctgcacaaat cgtcattgct
     9361 ggagaagtgg ttgcagttga tcgagcggtt gaacttttgc aagaagcagg tgccaaacgc
     9421 ttgattcctc ttaaggtgtc aggtcccttt cacaccgctc tccttgaacc tgctagccag
     9481 aaactagctg aaactctagc tcaggtaagt ttttcagatt ttacttgtcc cctagtcggc
     9541 aatacagaag ctactgtgat gcaaaaagag gacattgctc agctcttgac gcgtcaggtc
     9601 aaggaacccg ttcgtttcta tgaaagtatt ggggtcatgc aagaagcagg cataagcaac
     9661 tttatcgaga ttggaccggg gaaagtcttg tcaggttttg ttaaaaaaat tgatcaaact
     9721 gctcacttag ctcatgtgga agatcaagcg agtttagtag cacttttaga aaaatagact
     9781 aaaataagta gaagttttga aaggaaaaaa atgaaactag aacataaaaa tatctttatt
     9841 acaggttcga gtcgtggaat tggtcttgcc atcgcccaca agtttgctca agcaggagcc
     9901 aacattgtct taaacagtcg tggggcaatc tcagaagaat tgctcgctga gttttcaaac
     9961 tatggtatca aggtggttcc catttcagga gatgtatcag attttgcaga cgctaagcgt
    10021 atgattgatc aagctattgc agaactgggt tcagtagatg ttttggtcaa caatgcaggg
    10081 attacccaag atactcttat gctcaagatg acagaagcag attttgaaaa agtgctcaag
    10141 gtcaatctga ctggtgcctt taatatgaca caatcagtct tgaaaccgat gatgaaagcc
    10201 agagaaggtg ctatcattaa tatgtctagt gttgttggtt tgatggggaa tattggtcaa
    10261 gctaactatg ctgcttctaa ggctggcttg attggcttta ccaagtctgt ggcacgcgag
    10321 gtcgctagtc ggaatatacg agtcaatgtg attgctccag gaatgattga gtctgatatg
    10381 acagctatct tatcagataa gattaaggaa gctacactag ctcagattcc gatgaaagaa
    10441 tttgggcagg cagagcaggt tgcagatttg acagtatttt tagcaggcca agattatcta
    10501 actggtcaag tggttgccat tgatggtggc ttaagtatgt agcgaaagct agaggtgaaa
    10561 agaatgaaac taaatcgagt agtggtaaca ggttatggag taacatctcc aattggaaat
    10621 acaccagaag aattttggaa tagtttagca actgggaaaa tcggcattgg tggcattaca
    10681 aaatttgatc atagtgactt tgatgtgcat aatgcggcag aaatccaaga ttttccgttc
    10741 gataaatact ttataaaaaa agataccaac cgttttgata actattcttt atatgccttg
    10801 tatgcagccc aagaggctgt aaatcatgcc aatcttgatg tagaggctct taatagggat
    10861 cgttttggtg ttatcgttgc atctggtatt ggtggaatca aggaaattga agatcaggta
    10921 cttcgccttc atgaaaaagg acccaaacgt gtcaaaccaa tgactcttcc aaaagcttta
    10981 ccaaatatgg cttctgggaa tgtagccatg cgttttggtg caaacggtgt ttgtaaatct
    11041 atcaatactg cctgctcttc atcaaatgat gcgattgggg atgccttccg ctccattaag
    11101 tttggtttcc aagatgtgat gttggtggga ggaacagaag cttctatcac accttttgcc
    11161 atcgctggtt tccaagcctt aacagctctc tctactacag aggatccaac tcgtgcttcg
    11221 atcccatttg ataaggatcg caatgggttt gttatgggtg aaggttcagg gatgttggtt
    11281 ctagaaagcc ttgaatacgc tgaaaaacgt ggagctacta tcctggctga agtggttggt
    11341 tacggaaata cttgtgatgc ctaccacatg acttctccac atccagaagg tcagggagct
    11401 gtcaaggcca tcaaactagc cttggaagaa gctgagattt ctccagagca agtagcctat
    11461 gtcaatgctc acggaacgtc aactcctgcc aatgaaaaag gagaaagtgg agctatcgta
    11521 gctgttcttg gtaaggaagt acctgtatca tcaaccaagt cttttacagg acatttgctg
    11581 ggggctgcgg gtgcagtaga agctatcgtc accatcgaag ctatgcgtca taactttgta
    11641 ccaatgacag ctgggacaag tgaagtatca gattatatcg aagctaatgt cgtttatgga
    11701 caaggcttgg agaaagaaat tccatacgct atttcaaata cttttggttt tggaggccac
    11761 aatgcagttc ttgctttcaa acgttgggag aatagataag tatgaattta aacgatatta
    11821 aagacttgat gactcaattt gaccagtcaa gtttgagaga attttcttat aaaaatggga
    11881 cggatgagtt gcagtttagc aagaatgaag cgagacctgt gcctgaagtt gcaactcaag
    11941 tcgctccagc acccgttcta gcaacaccga gtccagtagc tcctacatct gctccagcag
    12001 agactgtagc agaagaagtt ccagctccag ctgaagcaag tgtggctact gagggaaatc
    12061 ttgtagagag tccacttgtt ggagtggttt acttggctgc tggaccagat aaacctgcct
    12121 tcgttacagt tggtgatagt gtcaaaaaag gtcaaacatt ggtaattatc gaagccatga
    12181 aagtcatgaa tgaaatccca gctcctaagg atggtgtggt aacggaaatt ctcgtctcta
    12241 acgaagaaat ggttgagttt ggtaaaggat tggtacgtat caaatgatcg atattcaagg
    12301 aatcaaagaa gcccttcccc accgttatcc tatgcttcta gtggaccgtg tcttggaagt
    12361 gagcgaggat accattgttg ctatcaaaaa tgtgaccatc aacgagcctt tctttaacgg
    12421 ccactttcct caatacccag ttatgccagg tgttctgatt atggaagcct tggcgcaaac
    12481 tgccggtgtg ttggagttat caaaacctga aaataaagga aaactggtct tttacgctgg
    12541 tatggacaag gttaagttta agaagcaagt tgtaccaggc gaccaattgg ttatgacagc
    12601 gacttttgta aaacgtcgtg gcaccatagc tgtggttgaa gcaaaggctg aagtggatgg
    12661 caagcttgca gccagtggta cccttacttt tgcaattggg aactaaggag gttctccatg
    12721 tttcgaaaaa ttttaattgc caatcgtggt gaaattgcgg ttcgtattat ccgtgcggca
    12781 cgtgaattgg ggattgcgac ggtagcggtt tattcaactg ctgataagga agctcttcat
    12841 acgcttttgg cagatgaagc agtttgtatt ggtcctggca aggcaacaga gtcttatctc
    12901 aatattaatg cagttctatc agctgcagtc ttgactgagg cagaagctat tcaccctggt
    12961 tttggatttc tcagtgaaaa ttccaaattt gcgaccatgt gtgaagaagt aggtatcaag
    13021 tttatcggtc catctggtca tgttatggat atgatggggg ataagatcaa tgcgcgtgct
    13081 cagatgatta aagcaggtgt gcctgttata ccaggttcag atggagaagt gcataactct
    13141 gaagaagctt tgattgttgc tgaaaaaatt ggctatcctg ttatgctcaa ggcttcagca
    13201 ggtggaggtg gtaaagggat tcgtaaggtt gaaaaaccag atgacctcgt ttctgccttt
    13261 gaaactgcct ctagtgaggc caaggccaat tatggcaatg gtgccatgta catagaacgg
    13321 gttatctatc cagctcggca cattgaggtt caaatcctag gtgatgagca tggacatgtg
    13381 attcacttgg gtgaacggga ttgttctctt caaaggaata accaaaaggt tttggaagaa
    13441 agtccctcga ttgcaatcgg aaaaacgctg cgtcatgaaa taggtgctgc tgctgttcga
    13501 gcggcagagt ttgttggcta tgagaatgca ggaaccattg aatttcttct tgatgaagca
    13561 agtagcaatt tctatttcat ggagatgaat actcgtgttc aggtagaaca tccagtaaca
    13621 gagtttgttt caggtgttga tatcgttaag gaacagattt gcattgcggc aggtcagcct
    13681 ttgtctgtta agcaagaaga tattgtccta cgtggtcatg ccatcgagtg tcgtatcaat
    13741 gcagaaaacc cagcctttaa ctttgctcca agtccaggta agattactaa tctctatctg
    13801 ccaagtggtg gagttggctt gcgcgtggat tcagcagttt atccaggtta taccattccg
    13861 ccttattatg atagtatgat tgccaaaatc atagtacacg gcgaaaatcg ttttgacgcc
    13921 ttgatgaaaa tgcaacgtgc cctctatgaa ttagagattg aaggagtgca gactaatgca
    13981 gatttccagc ttgacctcat ttcagatcgc aatgtcattg ctggggatta tgatacttcc
    14041 ttcttgatgg aaaccttctt acctaaatat caagaaaaag aataaaatga ctttaagagt
    14101 ttaaaccgaa aaggggaatc aatggctcta tttagtaaaa aagataagta tattcgaatc
    14161 aatcccaatc gttcggttag ggaaaaacct caagctaagc cagaggttcc agatgaatta
    14221 ttttcccagt gtccaggctg taagcatacc atctatcaga aggatctggg aagtgaacgt
    14281 atctgtccgc actgtagcta tacctttcgt atttctgccc aagaacgctt ggctttgacg
    14341 attgatatgg gaaccttcaa agaattgttt acagggattg aaagcaagga tcccttgcat
    14401 ttccctggtt accaaaagaa actggcatct atgcgtgaaa aaacaggtct gcatgaagcc
    14461 gttgtgacag gaactgctct tattaaaggt cagactgtgg ctcttgggat tatggattct
    14521 aactttatca tggcttctat gggtacggtt gtaggtgaaa aaatcactcg tttgtttgag
    14581 tatgcgactg tcgaaaaatt gccagttgtc ctattcacag cctctggtgg agcccgtatg
    14641 caggaaggaa tcatgagtct catgcagatg gctaagatct ctgcggcggt taaacgccat
    14701 tcaaatgctg gtctctttta cctgaccatt ttgacagatc caacgactgg tggtgtgaca
    14761 gcatctttcg ctatggaagg cgatatcatt ctggctgaac cacagagctt ggttggtttt
    14821 gctgggcgtc gtgtgattga aaatacggtt cgtgaaagct tgcctgagga tttccaaaag
    14881 gcagaattcc tattagaaca tggctttgtg gatgctattg tcaaaagaag agacttacca
    14941 gatacgattg ctagcctagt cagattgcat ggagggagtc ctagatgaat attgcaaaaa
    15001 tagtcagaga agcgcgtgag cagagtcgct tgacaacctt ggactttgcg acaggcattt
    15061 ttgatgaatt tatccaatta catggtgacc gttcttttcg tgatgatggt gcagttgttg
    15121 gtggtattgg ttggcttgga gaccaagctg taacagtggt tggtatccaa aaaggcaaga
    15181 gtttgcaaga caacctcaaa cggaattttg gccaaccaca tccagaaggc taccgaaagg
    15241 cactgcggtt gatgaaacag gctgagaaat ttggccgtcc agttgtgacc tttatcaaca
    15301 cagcaggtgc ttatcctggt gtcggagcgg aagaacgtgg tcaaggggaa gctatcgctc
    15361 gcaatctcat ggaaatgagt gacctgaaag ttcctattat cgccattatt atcggtgaag
    15421 gtggttcagg cggggctctg gctctagctg tcgcggaccg tgtctggatg ctggaaaatt
    15481 ctatctatgc cattctcagt ccagaaggct ttgcttccat tttatggaag gacggtactc
    15541 gcgccatgga agcagcagaa ctgatgaaaa tcacttcgca tgaactgtta gaaatggacg
    15601 tggtggataa ggtgatttct gaagtaggac tttctagtaa agaactgatt aagagtgtca
    15661 aaaaagaact ccaaacggag ctggctagac tttcacaaaa accgctagaa gagttgctgg
    15721 aagaacgcta tcaacgattt agaaaatact aagaatgact aaatgagact agaaaggtct
    15781 cattttttat ggaaaaatgc aattcaggtt gaaaaattga cagttgagga gatgggctat
    15841 aaaatagaca aaaaagatgt atactgtaga tgtaagctta caaaaat
//
LOCUS       DAVLTM010000041        15763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLTM010000041 DAVLTM010000000
VERSION     DAVLTM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.041"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..373)
                     /locus_tag="VYN16_001086"
     CDS             complement(<1..373)
                     /locus_tag="VYN16_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1403630.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIA"
     regulatory      402..498
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            496..1005
                     /locus_tag="VYN16_001087"
     CDS             496..1005
                     /locus_tag="VYN16_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1403631.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     gene            1105..1177
                     /locus_tag="VYN16_001088"
     tRNA            1105..1177
                     /locus_tag="VYN16_001088"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Thr,seq:ggt)
     gene            1763..2839
                     /locus_tag="VYN16_001089"
     CDS             1763..2839
                     /locus_tag="VYN16_001089"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1403632.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            2895..3830
                     /gene="dapA"
                     /locus_tag="VYN16_001090"
     CDS             2895..3830
                     /gene="dapA"
                     /locus_tag="VYN16_001090"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1403633.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLVYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFIAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(3877..5250)
                     /gene="mnmE"
                     /locus_tag="VYN16_001091"
     CDS             complement(3877..5250)
                     /gene="mnmE"
                     /locus_tag="VYN16_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1403634.1"
                     /translation="MITREFDTIVAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIVDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPEAIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNEGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5388..5570)
                     /locus_tag="VYN16_001092"
     CDS             complement(5388..5570)
                     /locus_tag="VYN16_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1403635.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            5694..6293
                     /locus_tag="VYN16_001093"
     CDS             5694..6293
                     /locus_tag="VYN16_001093"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1403636.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGFIRDLPETPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            6283..6756
                     /locus_tag="VYN16_001094"
     CDS             6283..6756
                     /locus_tag="VYN16_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403637.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKKYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTASAGLGNKLWLSYEPENEHARSCYLSYGFKETGEIYENEVVAIYDFNN"
     gene            6773..7852
                     /gene="prfA"
                     /locus_tag="VYN16_001095"
     CDS             6773..7852
                     /gene="prfA"
                     /locus_tag="VYN16_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1403638.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASTR
                     DTVTAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            7852..8691
                     /gene="prmC"
                     /locus_tag="VYN16_001096"
     CDS             7852..8691
                     /gene="prmC"
                     /locus_tag="VYN16_001096"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1403639.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTFKDQFGQDRMVVVDD
                     GQD"
     gene            8678..9277
                     /locus_tag="VYN16_001097"
     CDS             8678..9277
                     /locus_tag="VYN16_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1403640.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFDQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            9279..9710
                     /locus_tag="VYN16_001098"
     CDS             9279..9710
                     /locus_tag="VYN16_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403641.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            9772..11028
                     /locus_tag="VYN16_001099"
     CDS             9772..11028
                     /locus_tag="VYN16_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1403642.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYISLIEPGDTVMGMDLASGGHLTHGAPVSFPGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            11036..12010
                     /locus_tag="VYN16_001100"
     CDS             11036..12010
                     /locus_tag="VYN16_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1403643.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            12010..12624
                     /gene="pmp23"
                     /locus_tag="VYN16_001101"
     CDS             12010..12624
                     /gene="pmp23"
                     /locus_tag="VYN16_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1403644.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            12698..13672
                     /locus_tag="VYN16_001102"
     CDS             12698..13672
                     /locus_tag="VYN16_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1403645.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            13746..15377
                     /gene="rlmD"
                     /locus_tag="VYN16_001103"
     CDS             13746..15377
                     /gene="rlmD"
                     /locus_tag="VYN16_001103"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1403646.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKNS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSEQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            15531..15680
                     /locus_tag="VYN16_001104"
                     /pseudo
     CDS             15531..15680
                     /locus_tag="VYN16_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         4845 a         2969 c         3384 g         4565 t
ORIGIN      
        1 gtgcaattat tgtttcaata gcatttatct tatcaatgtt ttcagttacg aattgcccta
       61 tgatgtgagg aaattgggtt acaaagagaa aagttaaaaa caagtctaac agaaaagtag
      121 aaaggcgagc aagagaaaat ttcactaaac ggataagtct atttttcttt ttttgcttaa
      181 atacaatcgt atcatttgtg ataaaggcaa ataagatacc gataatattt gccaaggccg
      241 tagcaaggag ttcctgatga cttagttgat aaatgactag tcgtgagaga atcgaaacta
      301 gagttgtcgc aaggccaaaa aagagatagg ctaagatttc attgttaaaa aatttttgaa
      361 taggtttttt catgctttaa gtatagcata aagcaggcta ttgtgctata ctagtaaggt
      421 tgaatgaatc aacccttggt gcttagcttc tttcaccaag catattacac gcggataacc
      481 gccaaaggag aaaagatgaa aaaattaact attcgtgatg ttgcagatat tgcaatcgtc
      541 gctgctatct acgtcgtttt gactgtcaca ccgcccctaa atgccatcag ctatggtgct
      601 tatcagtttc gtatttcaga aatgatgaac tttatggctt tttacaatcc taagtacatc
      661 atcggagtta ccatcggttg tatgattgct aatttcttta gcttcggact gctagatgtc
      721 tttgttggtg gtggttcaac cttagtattc ctcagtttag gtgtttggct ttttgcaaaa
      781 tatagcaagg attacctctt taacggattg attcgaaaag atcatttctt cttttcaatc
      841 ctcttttcaa tttccatgtt tactatcgct gcagagttaa atattgtagc agaattacca
      901 ttcttcctta cttggtttac aacaggaatc ggtgaatttg cttcattgat tattggagcg
      961 attattattg gtaaaattgg tcgtcgaatt gatttaagta aatagaagaa gataagctag
     1021 gtagttctta cctggctttt tcctatggaa aatttctaag gaaacttgac aagttttttt
     1081 gaatatagta tacttcttaa gtaagctgat ttagctcagt tggcagagcg catccatggt
     1141 aaggatgagg tcgccggttc aatcccggca attagcatta aatagacaga aaagcccttg
     1201 atttacaagg gtttttgtta tgtctgcccc caatttttcc aatatatttc ttatgcacct
     1261 gtgtaaataa aactaatact caatgaaaat caaagagtaa actaggaaac tagccgcagg
     1321 ttgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagct caaaacatgt
     1381 ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat acatacggca aggcgacgct
     1441 gacgaagttt gaagagattt tcgaagagta taaagagttt cgtctgttga caaaagtcat
     1501 tctctttagt agaatagtag atgcgatgag tcgataggta gtcttcgggc tactattgag
     1561 cataaggagg tcataacgca ggagcggacc ttgatgagtt gtgtgaacct gctcatcaca
     1621 tgaagatgcc tcttaatccc tagtctagcg attggggatt tttattttcc aaaaaatgat
     1681 gaaaaagctt tgcaattcat ggaaaaagta gtataatact tctattatag aaatttttag
     1741 aaaattccga aagaggttat ttatgggata tacagttgct gtagtcggcg cgacaggtgc
     1801 tgtcggtgct cagatgataa aaatgttgga agaatcaaca cttccaatcg ataaaatccg
     1861 ttaccttgct tctgcacgtt cagcaggtaa gtcattgaaa tttaaagatc aagatattac
     1921 gattgaagaa acgactgaaa cagcttttga aggagttgat attgctctct tttcagcagg
     1981 tggttcgaca tcagctaagt atgcaccata cgcagtaaaa gctggcgtgg tagtagtgga
     2041 taatacatct tatttccgtc aaaatccaga tgttcctttg gttgttccag aggtcaatgc
     2101 tcatgcactt gatgcccaca acggaatcat tgcctgccct aactgttcaa caatccaaat
     2161 gatggtggct cttgagccgg ttcgccaaaa atggggcttg gaccgtatca ttgtttcaac
     2221 ttatcaagcc gtttcaggtg ctggtatggg agcaattctt gagacacaac gtgaacttcg
     2281 tgaggtcttg aatgatggag tgaaaccacg tgatttgcat gcggaaatct tgccttcagg
     2341 tggtgacaag aaacattatc ctatcgcctt taacgctctt ccacaaattg atgtcttcac
     2401 tgataatgat tacacgtacg aagagatgaa gatgaccaag gaaactaaga aaattatgga
     2461 agatgatagc attgcagtat ctgcgacatg tgtgcgtatt ccagtcttgt cagctcactc
     2521 tgagtcagtt tatattgaaa caaaggaagt ggctccaatt gaagaagtca aagcagctat
     2581 cgcatccttc ccaggtgctg ttcttgaaga tgatgtagtt catcaaatct atcctcaagc
     2641 catcaatgca gttggttcac gtgatacctt tgttggtcgt atccgtaaag acttggatgc
     2701 agaaaaagga attcacatgt gggttgtttc agataacctt ctcaaaggtg ctgcttggaa
     2761 ctcagttcag attgctgaaa ctcttcatga acgtggattg gttcgtccaa cagctgaatt
     2821 gaaatttgaa ttaaaatagt catatcgttt aggagttcag atgaactcct tctttgaaat
     2881 agagaggtgt tttcgtgtct tatcaagatt taaaaaaatg taaaatcatt acagctttta
     2941 ttaccccctt ccatgaggat ggttccatta actttgatgc tattccagcc ttgattgagc
     3001 atttattggc ccatcatacg gatggaattc ttctcgcagg aacgactgct gagagtccaa
     3061 ctttgaccca cgatgaggag ttggagttgt ttgcggctgt acaaaaggtt gtcaatggac
     3121 gcgttccttt gattgcgggt gtaggtacta atgatacgcg tgactctatt gagtttgtca
     3181 aagaagtagc ggaatttggt ggtttcgcag ctgggcttgc tattgttcct tactacaaca
     3241 aaccttctca agaagggatg tatcagcact ttaaggctat tgcagatgct tctgacctac
     3301 caattattat ctataacatt ccagggcgtg tggttgttga attaactcca gaaaccatgc
     3361 ttcgcttggc tgatcatcca aatattatcg gtgtcaaaga atgtactagc ttggctaata
     3421 tggcttactt gattgagcac aaaccagaag agttcttggt ctatactggt gaggatggag
     3481 atgctttcca tgccatgaac cttggggcgg atggggttat ttctgttgcc tctcatacaa
     3541 atggggatga aatgcacgag atgtttattg ccattgcaga aagtgatatg aagaaagccg
     3601 cagctattca acgtaaattc attcctaagg ttaatgctct cttctcttat ccaagtcctg
     3661 ctccagttaa ggcagttttg aactacatgg gatttgaagc tggaccaact cgtctacctc
     3721 ttgttccagc accagaagaa gatgctaaac gcattatcaa ggttgtcgta gatggcgact
     3781 acgaagcgac caaggcaact gtaacaggtg tcttaagacc agattactaa taaagacaat
     3841 aaaatccatg accgaatgaa ggatcatgga tttttcttat tttcctaaac agaattggct
     3901 aaagagttgg gtgataagtt catctggagc ggcatcccca gtgatttctc cgaggatttc
     3961 ccaagtacgt gtcaaatcta cttgaagcaa atcaactggc atccccagtt ctagaccctc
     4021 attaacagct tgtaagcttt caactgcctt ttcaatcaag gaaatatgac gggcattgga
     4081 caggtaggtt gcgtcttgct caaccaaacc agcattttca aagaagaggt tgttgattcg
     4141 ctcttcaatc ttatcgatgt tttggttttt aaggactgaa atacgaataa cgtcttcagg
     4201 tagttccgaa gtctcaatcg cttctggcag gtcggtttta tttagaagta taatgcggtt
     4261 tgtatcttgg ctaatctcaa gaagttgtcg gtcttgggct gtcagtggct cgctggcatt
     4321 tagtactaga agaaccaagt cagcttcctt gagggctttt ttcgaacgtt ccacaccgat
     4381 ttgttcaacg atgtcatccg tttcacgaat acctgctgtg tcaatcaatt tgagagggac
     4441 accgttgatg ttgacgtatt cttcgatgac atcacgtgtt gtcccagcaa tgtctgtgac
     4501 gatggccttg tcttcacgca agaggttgtt gagcaggcta gattttccaa cgttggggcg
     4561 gccgatgata gctgttgaaa ttccttcccg aagaatttta cctcgacggg ctgtcctaag
     4621 gagattagtt agcaattgct caaactccat agtcttctcg cggacaacgg cagtagtagc
     4681 ttcctcaaca tcatcgtatt caggatagtc gatattgact tcaacttggg caagtgtgtt
     4741 aaggatttct tgacgggtat tattgataag atcagaaagg gaaccatcaa gttgtttgac
     4801 cgcaatgttc atggccttgt ctgtcttagc gcggatgata tccatgaccg cctcagcctg
     4861 tgtcaaatct acgcgaccgt ttaaaaaggc acgtttggta aattcaccag gttcagccaa
     4921 ccgagctcct tcacggatag ctagctggag aatctcattg gtcaccgcaa tcccaccgtg
     4981 ggtgttaatc tcgataatat cctcacgagt gaaggttttt ggagatttca tggctcccac
     5041 catgacctcg tccatgactt taccagtcag aggatcaacg atgtgaccgt agttgagagt
     5101 gtggctagca accttgttca agtcttttcc tttaaaaatc ttttgcgcaa tagcaaaact
     5161 gtctgttccg ctcaggcgga caataccaat agccccttca cctagtggag tagagatagc
     5221 aacgatggta tcaaattcac gtgtaatcat aatatttcct ttaatcaata gctcttttct
     5281 tggaaaattc tccaagtctc ttttcaaatt gtaacaaatt tgaactattt cttcaatgaa
     5341 tgctacttgg agattgaaaa agcctaagca attctgctta agctagctta tttggtgcgc
     5401 atttcacctt gagggaagta agttccttct ggcatgtcgt tgatgatgac atggacagca
     5461 gattgagggg ctccagtgtt acggacaaca gcttccgtta cttccttagc aagagccttc
     5521 ttttgctcga gcgtgcgtcc ttcaaataaa tcgatgcgta caaatggcat aatagcttcc
     5581 tccactagtt tttgatttct tctattttac cacattttgc cgtttaaagc ttcagaaaat
     5641 tatgatatac tagaatgtag caaaaattta gaaacggacg tgaagcaaga aacatggcac
     5701 agttgtatta tcgttatggg accatgaact caggtaagac gattgagatt ctcaaggtgg
     5761 cctataacta tgaggagcaa ggaaaaggtg ttgtgattat gacctcggct ctggatacgc
     5821 gtgacggtgt tggctatgtg tcgagtcgaa tcggtatgaa acgccctgct cttgcgattg
     5881 aggagacgac ggatatcttt ggatttatcc gtgatttacc agaaacacct tactgtgtgt
     5941 tggtcgatga agcccagttt ctcaaacgtc atcatgttta cgatctagct cgtgttgtgg
     6001 acgagttaga tatacccgtc atggcttttg gtttgaaaaa tgattttcgc aatgaactgt
     6061 tcgaaggttc caagtatctc ttgcttttag cagataagat tgatgaaatc aagaccattt
     6121 gtcagtattg taagaaaaag gcaactatgg ttttgcgaac acaggatgga ctgcccgttt
     6181 acgatggaga acagatccag attggcggta atgaaaccta tatctcggtt tgccgtaaac
     6241 attattttgc gccaatgata acctctaaca aggagcaaaa ctatgacaat tgaactaaga
     6301 gatgttacaa tggaaaatta ttttgatgtt ttgaatttgg atgtcaagaa atatcaaaaa
     6361 caattcattg caaccaacgc aattagttta gctgaagcat atgtctacac taaaaatgga
     6421 gattttgtag ctccattggc agtttatgat aatgatgcaa ttataggttt tgtgatgata
     6481 gcttatgata aaaagatcgg aataagtagt ggaaattatt tactatttcg ttttatgatt
     6541 gataagaatt ttcaaaatca aggatatttt aaaccaatta tggataaagt actagactat
     6601 gttcggacag cgtcagcagg tttaggcaat aaactttggt tgtcttatga accagaaaat
     6661 gaacatgcaa gatcttgtta cctcagttat ggatttaaag aaactgggga aatatacgag
     6721 aacgaagtag tagcgatata cgattttaac aattgagaaa taaggagaaa aaatgaacat
     6781 ttatgatcaa ctacaagctg tagaagaccg ttatgaagaa ttaggagaat tgctcagtga
     6841 cccggatgtc gtttcagaca ccaagcgttt tatggagctt tcaaaagaag aggcttcaac
     6901 tcgtgacacg gttacagcct accgtgagta caaacaagtc cttcaaaata tcgtcgatgc
     6961 tgaagagatg attaaggaat caggcggaga tgcggacttg gaagaaatgg ccaagcaaga
     7021 gctcaaagat gccaaggctg aaaaagaaga atacgaagaa aaactaaaaa tcttgctcct
     7081 tccaaaggat ccaaacgatg acaagaatat catccttgaa atccgtggag cagctggtgg
     7141 agacgaagcg gcacttttcg ctggagattt gctaactatg taccaaaagt atgcggaagc
     7201 ccaaggttgg cgctttgaag tcatggaagc ctctatgaat ggtgtcggtg gttttaaaga
     7261 agtggttgct atggtttcag gtcagtctgt atactctaag cttaagtatg aatcaggtgc
     7321 ccaccgtgtg caacgtgttc ctgtgacaga aagccaaggc cgtgttcata cttcgacagc
     7381 gacagttctt gttatgccag aagttgaaga ggttgaatac gacattgatc caaaagacct
     7441 tcgtgtcgac atctatcacg cctctggtgc tggtggacag aacgtcaata aggttgcgac
     7501 tgccgttcgt atcgttcact tgccaaccaa tatcaaggtt gagatgcagg aagaacgtac
     7561 ccagcagaag aaccgcgaga aggctatgaa gattatccgt gcacgcgtcg ctgaccactt
     7621 tgctcagatt gctcaggatg aacaagacgc tgagcgtaag tcgacaatcg gtactggtga
     7681 ccgttcagaa cggatccgaa cttataactt cccacaaaac cgtgtcacag accaccgtat
     7741 cggcttgacc ctccaaaaac tagatacgat tttgtctggt aaattggacg aagttgtgga
     7801 tgccttggtg ctttatgacc aaacacaaaa actagaagaa ttaaacaaat aatgaaatta
     7861 gctcaattat tttcaaattt tgaagaagag ttgataagac aaggagagga agcggaaagc
     7921 ctctcttttg tctatcgtag tctgaaaaat ctatctttta cagactttat ttttgccctc
     7981 cagcaggagg tgacaacaga ggaagaaaaa caatttgtag aagatattta ccagcaacta
     8041 gcagctcaca agccagctca gtacatcatt ggacaggcag atttttatgg aatgcattta
     8101 aaggtggatg aacgggtttt gattcctcgt ccagaaacag aggagttagt ggagcttatt
     8161 ctaactgaaa atcttgagac gaatctttca gttctggata ttggaacagg tagtggagct
     8221 attgctctcg ctctagcaaa aaatagacca gattggtcag tgacagcagc agatgtttct
     8281 caagaggctt tagatttagc tagcgaaaat gctaaaaatc aaaatcttca aatatttttt
     8341 aaaaaatctg actgttttac agaaatttct gaaaaatatg atataattgt ttccaatcca
     8401 ccctatatct ctcgtgaaga tgagtcagag gtaggcttga atgtcttgta ttcggagcct
     8461 catctagctc tctttgcaga cgaggatggc ctagctattt accgtagaat tgcggaagat
     8521 gcaaaagact atctcaaaga tggtggtaag atttaccttg aaattggata caagcaaggt
     8581 caaagtgttc ccgaactttt tagaaaacat cttcctgaaa aaaaagttcg aacattcaag
     8641 gaccaatttg gtcaagatag gatggtcgtg gttgatgatg gacaggatta gacaagagtt
     8701 ggaaaatggt ggagctgtca ttctgcctac agagacggtt tatggtcttt ttgccaaggc
     8761 cttagacgaa aaagcagttg accatgttta ccagctcaaa cgtcgtccta gagacaaggc
     8821 gctcaatctc aatgttgcct ctttagagga tatcttgcac ttttctaaga atcagccagc
     8881 ttatctacaa aaacttgtag agaccttttt gccaggtccc ttgaccatta ttctcgaagc
     8941 caatgaccga gttccctatt gggtaaattc tgaccttgca actattggat ttcggatgcc
     9001 cagtcaccct atcacactgg atttaattcg agagacaggt cccttgattg ggccgtctgc
     9061 caatatctca ggtcaggcaa gtggtgtaac ctttgatcaa attctaaagg attttgacca
     9121 agaggttctg ggtctggaag acgatgcttt tctaactgga caggattcaa ctattgtgga
     9181 tttgtctgga gacaaggtga aaatcttacg ccaaggcgca attaaacgag aagatattct
     9241 tgctcggttg ccagagattt cttttgagga ggcttgaaat gctaagagat ttgcaagaaa
     9301 cagatgtgaa agcgatatgt gacatcaacc aagaggcttt gggttatact tttagtccag
     9361 aggaaacggc tagccaacta gctagactgt ctcaggattc ccatcatttc ctacttggct
     9421 atgaggatgc agctaatcat gtcttacttg gatatgtcca cgctgaagtt tacgaatcac
     9481 tctattccaa agcaggattt aatatcttag ctttagcagt ttcacctcaa gcgcaaggtc
     9541 aaggtatcgg taaaagttta ctacaagggt tggaacaaga agccaaaaga tgtggttatg
     9601 ggtttatccg cttaaattct gccaatcatc gtctgggtgc tcatgcattt tatgaaaaag
     9661 ttggctatac ttgtgataaa atgcagaaac ggtttattcg catcttttag tttgattttc
     9721 ttattgtaaa atcaaactaa tggactagtc acacaataaa ggagaagacc tatgattttt
     9781 gacaaagatg attttaaagc atatgatgct gatctctgga atgctattgc caaagaagaa
     9841 gaacgccaac aaaataatat cgagttaatt gcttcggaaa acgtagtttc caaggctgtt
     9901 atggcagctc aagggtctat cttgacaaat aaatatgccg agggttaccc aggacgccgt
     9961 tattatggtg gaactgatgt agtagacgtt gtagagactc ttgctattga acgcgcaaaa
    10021 gaaattttcg gtgctaaatt tgccaatgtc caaccacatt caggaagtca ggccaactgt
    10081 gcggcttaca tatccttgat tgagccaggt gatacggtta tgggaatgga tttggcatca
    10141 ggtggtcatt tgactcatgg ggctcctgtt agcttccctg gtcaaaccta caactttgtt
    10201 tcttatagtg ttgatcctga aacggaactc ttagactttg atgctatctt gaaacaagcc
    10261 caagaagtaa aaccaaaact gattgtagct ggtgcttcag catattctca aattatcgat
    10321 ttttcaaaat tccgtgaaat cgcagatgct gtcggtgcga agctcatggt ggacatggcc
    10381 catatcgctg gtttggttgc ggctggcctt catccaagcc cagttccata cgctcatatc
    10441 acaacaacaa cgacccacaa aacccttcgt ggacctcgtg gtggtttgat tttgaccaat
    10501 gacgaagaac ttgctaaaaa aattaattca gctattttcc caggtattca gggcggtcct
    10561 ttagagcatg ttgtggcggc taaggcagtt tccttcaaag aagttttgga tccagccttc
    10621 aaggaatatg ctgccaatgt aattaagaac agcaaggcta tggcagatgt cttcttgcaa
    10681 gaccctgatt tccgtattat ttcaggtgga actgaaaacc atctcttcct tgttgatgtg
    10741 actaaggttg tagaaaacgg aaaagttgct caaaacttgc tggatgaagt caatattacc
    10801 ctaaataaaa attcaatccc ttacgaaacc ttgtcaccat tcaagacaag tgggattcgt
    10861 atcggagcag cagctattac tgcacgtgga tttggtgaag aagaaagtcg caaagtggct
    10921 gaactcatca ttaaaaccct taagaattca gaaaatgagg ctgtattaga agaagtgaga
    10981 agtgcagtca aagaattgac agatgccttc ccattatacg aggaataaaa cttttatgga
    11041 catttatatt aagaaagcca ttattcacca gttcagtccg gatgataccg agctgttctt
    11101 agcagataag tttctcaata ttactccaaa aatcgaagaa tacctacgta aaaaaattga
    11161 acatgtgtat tcagatgaag ccaagactgg gattttcgaa gaagaaaatc ccttcttcaa
    11221 tcatattaca gacgatttgt tggagacatc agtaacgctg gctaatctct ggaaagagga
    11281 gtttagcatt tctgaaaatc tcaagaccaa tgacttgatt tttgttcaat tttctaaaga
    11341 aggtgtagaa catttcgctt tcttgcgaat tgccctgcgg gagaccttga cccacctcgg
    11401 aggagaagtt gataatccaa tcaagctaac tcagaataac ctgcctggat ttggaacagg
    11461 agctgatgag gccttggtgg tcaatcttca gagtagcaag tatcacctga ttgaaaaacg
    11521 aatcaagtac aacgggactt ttttgaacta tttttcagat aatcttcttg ctgtcgctcc
    11581 taagatttct cctaaaaaat ctatcaagga actggaaaaa acagcccaga gaattgctga
    11641 atcttttaac acagatgatt ttcaatttca atccaaggtc aaatcagcta ttttcaacaa
    11701 cctagaagaa agcaatgaat tgtcacctga gaaattggct aatgaccttt ttgacaacaa
    11761 tctgacggct cgtttgagct ttattgacca agtcaaagaa gccgtaccag aacctgttca
    11821 atttgatgaa attgatgtca gtcgccaatt aaagaaattt gaaaaccaaa aactctcctt
    11881 atcaaatgga attgagctca tcgttcccaa taacgtctat caagacgccg agtctgttga
    11941 gtttatccaa aacgaaaatg gaacctactc tatcttaatc aaaaatatcg aggatatcca
    12001 aagtaaataa tgtttaaacg aattcgaaga gtgcttgtac tagcagtctt cctttttgct
    12061 ggctataaag cttaccgcgt tcatcaagat gtcaaacaag tcatgaccta tcaacccatg
    12121 gtgcgagaaa tcttgagtga acaagacacc ccagcaaacg aagagcttgt gcttgctatg
    12181 atttatactg aaacaaaagg aaaagaaggc gatgttatgc agtctagtga gtctgcaagt
    12241 ggttccacca acaccatcaa tgataatgcc tctagcattc ggcaaggcat tcaaactctg
    12301 acaggcaatc tctatctggc gcagaagaag ggtgtagata tctggacagc tgttcaagcc
    12361 tataattttg gacctgccta tatcgatttt atcgcccaaa atggcaagga aaataccttg
    12421 gctctagcca aacagtactc tcgtgagact gttgccccct tgcttggtaa tacgactgga
    12481 aagacttata gttatattca tcccatttcc atttttcacg gtgctgaact ctatgtaaat
    12541 ggaggaaact attattattc tagacaggta cgacttaacc tttacatcat caaatgtttc
    12601 actctctttt caacatctgg ctagaccagg tgtttttgtt ataagttttc tttaagatag
    12661 atatattact ctaaagaagt aaaggaggaa attccctatg agaaagaaac tctttctgac
    12721 tagtgctgcg gtcttgtggg cagtaacagc tatgaatagc gtccatgcag caacagatgt
    12781 tcaaaaagtt atcgatgaaa cctatgtcca acctgaatat gtcctaggtt cctccctatc
    12841 tgaagaccaa aaaaatcaaa ctcttaaaaa actgggctac aatgcctcaa cagataccaa
    12901 agaactcaag accatgacac ctgatgttta ttctaaaatc atgaatgtgg ccaatgactc
    12961 tagcttacag ttgtattcat cagccaagat tcaaaagcta ggtgacaaat cgccacttga
    13021 ggtcaagatt gaaacacctg aaaacatcac taaggtaact caggatatgt accgaaatgc
    13081 agccgtaact ttgggtgtgg aacacgccaa aatcactgta gcagcaccca ttccagttac
    13141 aggtgagagt gctttggctg ggatctacta ttcgctagaa gctaatggag ccaaggtgcc
    13201 gcaagccaat aaagatttgg ctcaagaaga gctaaaagct ttgtcagata tcaatgctga
    13261 aaacaaggac aaatcaggct atgatgctaa taaattaaac gttgccctag ctgatatcaa
    13321 gtcaggactc gccaaagcta aagaaagcaa gggaaatctg acagaagaag atatccgcaa
    13381 gattgttgaa gataccttaa aaaattacaa acttgatcag gtcataacag gaaaccagat
    13441 caatatcatc atcaattttg ccttgaatct ctcaaagagt gatatcctca gcaatgcaga
    13501 tttcactaaa accctaaatg accttaaaca aagcatcgta tcacaagctg gcgacagttt
    13561 taaaaatatc aaccttaact ttgatgcgga taaggcgcta gaggacggtg gtaacttctt
    13621 aagctccctc tggcaagccc ttgtcaactt cttcaagagt tttggttctt aagaaatttc
    13681 atggtataat ggatggtaac cgtaacgttg ccgtcttttt tgtcggccaa tagaaagaga
    13741 aaagaatgtt aaagaaaaat gatattgtag aagttgaaat tgttgatttg acccatgaag
    13801 gggcaggagt tgccaaggta gatggtttgg tcttttttgt agagaatgct ttaccgagtg
    13861 aaaaaattct catgcgtgtc ctcaaggtca ataaaaagat tggctttgga aaagttgaaa
    13921 aataccttgt ccagtcacca caccgtaatc aagatctaga tttggcttac ctgcgttcag
    13981 gaatcgcgga tttaggacac ctttcttatc cagaacagct caagtttaaa accaagcaag
    14041 tcaagaacag tctctacaag attgctggaa ttgcagatgt agaagttgct gaaacgcttg
    14101 gtatggaaca tccagtcaag tatcgcaata aggcgcaggt gcccgttcgt cgagtgaatg
    14161 gtgtcttgga aacaggattt ttccgtaaga attcgcacaa cctcatgccc cttgaagatt
    14221 tctttatcca ggatcctgtc attgaccaag tcgtagtagc tcttcgagac ctgctccgtc
    14281 gttttgattt aaaaccttat gacgaaaagg aacagtctgg attgattcgg aatcttgtgg
    14341 tgcgtcgtgg tcactattca gaacaaatca tggtcgtttt ggtgacaact cgtccaaaag
    14401 tttttcgtgt tgaccaattg attgaacaag ttatcaagca gttcccagag attgtgtctg
    14461 tcatgcaaaa tatcaacgac cagaatacca atgcgatttt tggtaaggag tggcgcactc
    14521 tttatggtca agactatatt acggaccaga tgttgggaaa tgacttccaa atcgctggcc
    14581 cagcctttta ccaagtcaat actgaaatgg cggagaaact ctatcaaaca gccattgact
    14641 ttgcagagtt aaaaaaagat gatgtgatta ttgatgccta ttctggtatt ggaactattg
    14701 gtttatcagt cgccaagcat gtcaaagaag tctacggtgt tgaactgatt ccagaagcgg
    14761 ttgagaatag taaaaaaaat gctcagctga acaatatttc aaacgcccac tatgtctgtg
    14821 acacagctga aaatgctatg aagaattggc ttaaagatgg gattcaacca accgttatct
    14881 tggttgatcc tccacgcaag ggcttgacag aaagctttat caaagcaagc gcccaaacag
    14941 gagccgatcg catcgcctat atctcctgca atgtagcaac catggcgcgt gatatcaaac
    15001 tctaccaaga attgggatat gaattgaaga aagtccagcc ggtggatcta tttcctcaaa
    15061 cgcatcacgt cgagacggta gcacttttgt ccaaactcga tgtcgataag cacataagtg
    15121 ttgaaattga gctggatgag atgaatttga caagtgcgga gagcaaagca acatatgctc
    15181 aaatcaaaga atatgtttgg aataaatttg aattaaaagt ttcgacatta tatattgcac
    15241 agataaaaaa gaaatgtgga atagaattac gagaacatta caacaagtct aaaaaggata
    15301 aacaaattat tccacagtgt acacctgaaa aagaagaagc catcatggat gctttgagac
    15361 acttcaaaat gatttaatag aaaagaatga cagtatatga ctttctgcat ttattacatt
    15421 cctacttggt ataggaacag ctattattcc tttcttgcaa ggtatcaatt agaaaatagg
    15481 ctcaatataa agattgatag gatcattttt atatttaaag gagtgttgaa atgattgata
    15541 aaggcaacaa aaaattttag gataaatttg ctaagttgta tgcccctttt atgaaaaaag
    15601 ataaagaggt ttatgataaa gtttgtgaat atcttagtcc tcatttgaat aaagataagg
    15661 aggtgcttga acttgcttgt tggtttcgtg tcataatagt tatagaggca aatagttatg
    15721 taaatataag gagttcaaga cttctaccaa agtttaaaac tca
//
LOCUS       DAVLTM010000042        15628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLTM010000042 DAVLTM010000000
VERSION     DAVLTM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.042"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            343..2130
                     /locus_tag="VYN16_001105"
     CDS             343..2130
                     /locus_tag="VYN16_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1403647.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFKTLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2127..2603
                     /locus_tag="VYN16_001106"
     CDS             2127..2603
                     /locus_tag="VYN16_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1403648.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2631..3536
                     /locus_tag="VYN16_001107"
     CDS             2631..3536
                     /locus_tag="VYN16_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1403649.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3523..4338
                     /locus_tag="VYN16_001108"
     CDS             3523..4338
                     /locus_tag="VYN16_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1403650.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4345..4749
                     /locus_tag="VYN16_001109"
     CDS             4345..4749
                     /locus_tag="VYN16_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403651.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5043..6209
                     /locus_tag="VYN16_001110"
     CDS             5043..6209
                     /locus_tag="VYN16_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1403652.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVMITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6325..7362
                     /locus_tag="VYN16_001111"
     CDS             6325..7362
                     /locus_tag="VYN16_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1403653.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7569..8423
                     /locus_tag="VYN16_001112"
     CDS             7569..8423
                     /locus_tag="VYN16_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1403654.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGEKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            8573..8813
                     /locus_tag="VYN16_001113"
                     /pseudo
     CDS             8573..8813
                     /locus_tag="VYN16_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
     gene            complement(9144..9236)
                     /locus_tag="VYN16_001114"
                     /pseudo
     CDS             complement(9144..9236)
                     /locus_tag="VYN16_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9272..9568)
                     /locus_tag="VYN16_001115"
     CDS             complement(9272..9568)
                     /locus_tag="VYN16_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1403655.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(9558..9719)
                     /locus_tag="VYN16_001116"
     CDS             complement(9558..9719)
                     /locus_tag="VYN16_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1403656.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9789..10553
                     /locus_tag="VYN16_001117"
     CDS             9789..10553
                     /locus_tag="VYN16_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1403657.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLVGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(10793..10945)
                     /locus_tag="VYN16_001118"
     CDS             complement(10793..10945)
                     /locus_tag="VYN16_001118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403658.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            11163..12542
                     /locus_tag="VYN16_001119"
     CDS             11163..12542
                     /locus_tag="VYN16_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1403659.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12556..13221
                     /locus_tag="VYN16_001120"
     CDS             12556..13221
                     /locus_tag="VYN16_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1403660.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13351..13431
                     /locus_tag="VYN16_001121"
                     /pseudo
     CDS             13351..13431
                     /locus_tag="VYN16_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            13577..14026
                     /locus_tag="VYN16_001122"
     CDS             13577..14026
                     /locus_tag="VYN16_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1403661.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVEILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGVVNCGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            14183..14593
                     /locus_tag="VYN16_001123"
     CDS             14183..14593
                     /locus_tag="VYN16_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1403662.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14709..>15628
                     /gene="pavB"
                     /locus_tag="VYN16_001124"
     CDS             14709..>15628
                     /gene="pavB"
                     /locus_tag="VYN16_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1403663.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRSNGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVK"
BASE COUNT         4391 a         2948 c         3546 g         4743 t
ORIGIN      
        1 tacctctatt ttccttctcc atagaaaatt ttaccataaa agcgaaaaaa atagtagtct
       61 tttgaataaa aaattagaat aatagtttga aaaattaagc gatataggga ttgctcatat
      121 tttatcaatg tattctatac aaaattattt taggtcctat attccgaatt taaagagcgt
      181 ttgaaatggg tctaaaaatg tttgaaacta attgaatgtg tgtataatag taaaaatgca
      241 gtaaatatcc cgacaatttc tattgacaat tcaaacagat tggtttataa ttaatataac
      301 aacaaatgaa agcgcaaact ttcgcggtcg gaaggtagtt ttatgacacg atttgagata
      361 cgagatgatt tctatctcga tggaaaatca tttaagattt tatctggtgc cattcattat
      421 tttagggttc ctccagagga ttggtatcat tcgctctata acttgaaggc tcttggtttt
      481 aatacggtag agacttatgt tgcttggaat ttacacgagc ctcgtgaagg tgagtttcat
      541 tttgaaggtg atctggattt agagaaattt ctccaaatag cgcaggattt gggtctctac
      601 gcaattgtgc gtccgtctcc atttatctgt gcggaatggg aattcggtgg cttaccagct
      661 tggctcttga ccaagaacat gcgaattcgc tcatccgacc cagcatatat cgaggcagtt
      721 ggtcgctact atgatcagtt attgccaaga ctggtgcctc gtttgttgga caatggtggc
      781 aatattctca tgatgcaggt tgaaaatgag tatggttctt acggagaaga taaggcttac
      841 ctgagagcga ttcgacagct aatggaagag tgtggcgtaa cctgtcccct ctttacatca
      901 gatggtccat ggcgagctac tctgaaagct ggaaccttaa ttgaagagga cctctttgta
      961 acaggaaact ttggttctaa ggcaccttac aacttttcgc agatgcagga attctttgat
     1021 gaacatggta agaaatggcc actcatgtgt atggagttct gggatggttg gttcaatcgc
     1081 tggaaagaac cgattatcac acgggatcct aaggaattgg cagatgcagt tcgagaggtt
     1141 ttggaacaag gctctatcaa tctttacatg ttccacggtg gtacaaactt tggtttcatg
     1201 aatggttgct cggctcgagg aactttggac ctgccacaag ttacgtctta tgattacgat
     1261 gcccttctgg atgaagaagg aaatccaact gctaaatatc ttgcagtcaa gaagatgatg
     1321 gcaacacatt tttcagagta tccgcagttg gaaccactct acaaagagag tatggagttg
     1381 gatgctattc cactagttga aaaagtttct ttatttaaaa ccttagatag cttgtcaagt
     1441 cctgtagaaa gtctctatcc tcaaaagatg gaggagctgg gacaaagtta tggctaccta
     1501 ctttatcgaa cagaaacaaa ctgggatgca gaagaagaaa gacttcgtat cattgatggt
     1561 cgagataggg cccagctgta tgtcgatggt cagtgggtta aaactcaata tcagacagag
     1621 attggggaag atatttttta tcaaggtaaa aagaaagggc tatctaggtt agatatcttg
     1681 atagaaaata tggggcgtgt caactatggg cataagttct tagcggatac gcaacgtaag
     1741 ggaattcgga caggggtctg taaggatctg catttcttac taaactggaa acactatcca
     1801 ctcccactag acaatcctga gaaaattgat ttttcaaaag gatggactca aggacaacca
     1861 gccttttacg cttatgactt tacagtcgaa gagccaaaag atacttacct agacttgtct
     1921 gagtttggta agggggttgc ctttgtcaat gggcagaatc taggacgttt ttggaacgtt
     1981 ggcccaactc tctcacttta tatccctcat agctatctca aggaaggtgc caaccgcatc
     2041 attatctttg aaacagaagg tcaatataaa gaagagattc atttaactcg taaacctaca
     2101 ctaaaacata taaaggggga aaacttatga caattgtagg atgccgtatt gatggacgtt
     2161 tgatccacgg acaagtagcc aatctttggg ctggaaaact aaatgtttca cgcattatgg
     2221 ttgtagacga cgaagttgtc aacaacgata ttgaaaagag tggtttgaaa cttgcgacac
     2281 caccaggtgt gaaattgagt attttgccag ttgagaaagc tgcagccaat attcttgctg
     2341 gcaaatacga tagccaacgt ctctttatcg tggctcgtaa accagaccgc ttccttggtt
     2401 tggtagaagc aggtgtacca cttgaaaccc ttaatgttgg gaatatgtct caaacaccag
     2461 aaactcgttc tattacacgt tctatcaacg tagtagacaa ggatgtggaa gacttccaca
     2521 aactggcaga aaaaggtgtt aaacttactg ctcagatggt tccaaatgat ccaatttcag
     2581 actttttgag cttattaaaa taggaaaaaa atttttagga ggtcattgtt atgatacaat
     2641 ggtggcaaat tttacttctc actttgtact cagcttatca aatctgtgat gagttgacga
     2701 tcgtttcatc tgcaggttcc cctgtatttg ctggtttcat tactggttta atcatgggag
     2761 atgtgactac tggtttactt atcggtggta acttgcaact gttcgttctt ggggttggta
     2821 ctttcggtgg tgcttctcgt atcgacgcaa cttctggtgc ggttcttgcg acagccttct
     2881 ctgtttcaca aggaattgat gcaccgcttg ccattactac aatcgctgta ccagtagcag
     2941 ctctcttgac ttacttcgac gttcttggtc gtatgactac taccttcttc gctcaccgtg
     3001 tggatgctgc aatcgaacgc tttgactata aaggtattga acgcaactac ttgcttggtg
     3061 cgattccgtg ggctctatct cgtgcccttc cagtcttctt tgcccttgct tttggtggtg
     3121 cctttgtaca atcagtagta gacttcgttg aagcctacaa atgggttgca gatggcttga
     3181 cacttgcagg acgtatgctt ccaggtcttg gatttgcaat cttgcttcgt taccttccag
     3241 ttaaacgtaa ccttcactac cttgctatgg gatttggttt gacagctatg ttgactgttc
     3301 tttactcata tgtaacaggt cttggtggcg ctgttgctgg tatcgtaggt actcttcctg
     3361 ctgaagttgc tgaaaaaatt ggtttcgtga acaacttcaa aggtttgtct atgattggta
     3421 tttctatcgt aggtattttc cttgcagtgc ttcacttcaa aaatagccaa aaagtagctg
     3481 tagcagcacc ttctacacca tcagaaagtg gggaaatcga agatgacgaa ttctaattac
     3541 aaacttacaa aagaagattt taatcaaatc aacaaacgta gcttgtttac tttccaatta
     3601 ggttggaact acgaacgtat gcaagcttct ggttaccttt acatgatctt gcctcagttg
     3661 cgtaaaatgt atggtgatgg aactcctgaa ttgaaagaaa tgatgaaagt tcatactcaa
     3721 ttcttcaata cttcaccatt cttccatacc attatcgctg gttttgacct tgccatggaa
     3781 gaaaaagatg gtgtaggttc aaaagacgcc gttaacggta tcaagacagg tttgatggga
     3841 ccattcgctc ctcttgggga tacaatcttt ggttcacttg tacctgctat catggggtca
     3901 gtcgcagcaa ctatggctat cgctggccaa ccttggggga tcttcctttg gattgcagtt
     3961 gcagtagcgt atgacatctt ccgttggaaa cagttggaat ttgcttacaa agaaggggtt
     4021 aaccttatca acaacatgca aagtaccttg acagctttga ttgacgctgc atctgtactt
     4081 ggtgtcttca tgatgggtgc tcttgtagca acagtgatta actttgaaat ttcttacaag
     4141 ttgccaatcg gtgaaaagat gattgatttc caagacatct tgaaccaaat cttcccacgt
     4201 ttgcttccag caatctttac tgcctttatc ttctggttgc ttggtaaaaa aggtatgaac
     4261 tctactaaag ctatcggtat tattatcgta cttgctttgg ctctttctgc ccttggtcac
     4321 tttgcacttg gaatgtaatt ccttatgact aaatcattaa ttttggtgag ccatggtcgc
     4381 ttctgtgagg agcttagagg tagcacagaa atgattatgg gcccacaaga caacatttac
     4441 acagtagctc ttcttccaga agatggccca gaagaattta ctgctaaatt tgaagctgtt
     4501 attgaagggt tggatgattt cctagtcttt gcggatcttc tcggtgggac accttgtaat
     4561 gtggtgagtc gcttgatcat ggaaggtcgt gatattgacc tttacgcagg gatgaatctt
     4621 ccaatggtga ttgaatttat caatgcgagc cttacaggcg cagatgcgga ctacaagagc
     4681 cgtgctgcag aaagcattgt gaaagttaat gacctgttag cgggcttcga tgatgacgaa
     4741 gatgaataat actcttcgaa aatctcttca aactacgtca acgtcgcctt gccgtaggta
     4801 tatgttactg acttcgtcag tcttatccgg caacctcaaa acggtgtttt gagctgactt
     4861 cgtcagttct atctgcaacc tcaaagcagt gctttgagca gcctacggct agtttcctat
     4921 tttagttgga actcgattca attcatgtga caacgtgaaa atcgttagag cattttatat
     4981 agaatataca tgggaatgta gcttactccc attcccatat ttaatagaaa aagaggaact
     5041 caatgctaca ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc actacgcgtg
     5101 aaatctacca acagcctgat gtatggagag aagcttttga attttatcaa gcaaaacgtg
     5161 aagaaattgc agcctttcta caagaaatcg ctgataaaca tgactatatt aaggttatct
     5221 tgacaggtgc tgggacttct gcttatgtgg gagatacctt gctaccttac tttaaggaag
     5281 tctatgacga acgcaaatgg aatttcaatg ctattgcgac aacagatatc gttgccaaac
     5341 cagcaaccta tttgaaaaaa gatgtggcaa ctgtccttgt gtcttttgct cgtagtggga
     5401 actcgcctga aagtgtggcg actgttgatt tggccaaatc cttggtggat gagctttatc
     5461 aagtgatgat tacttgtgca gcagatggta aattggctct tcaagctcac ggtgatgacc
     5521 gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc atgacttcta
     5581 gctttacgtc tatgatgttg acagctctct tggtctttga tcctacagaa tttgctgtta
     5641 agtctgaacg ttttgaagtt gtatctagtc ttgcccgtaa agttttagac aaggcagaag
     5701 atgtcaaaga gctcgttgat ttagacttta accgtgtcat ctatctaggc gctggtcctt
     5761 tctttggact tgctcatgaa gctcagctca agattttgga attaactgct ggtcaagttg
     5821 cgaccatgta tgaaagccca gttggcttcc gtcacggtcc aaaatctctt atcaacgaca
     5881 atacagttgt tttggtcttt ggtacaacga cagactacac tcgtaagtac gacttggact
     5941 tggttcgtga agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg agtgatcaag
     6001 cttttggtct tgaaaatgtc aaagaagtgg cccttggttg tggcggtgtc ttgaatgata
     6061 tttaccgtgt cttcccttac atcgtttatg cccaactctt tgccctattg acttcactca
     6121 aggtagaaaa taaaccagat acaccgtctc ctacaggtac agtaaaccgt gtagtacaag
     6181 gtgtcataat tcacgaatat caaaagtaag acagtgttta tgaattcttg acaagaggat
     6241 ttgtaaatta tcagataaac catagattgt cagtacgctt tctatggttt gtttgcttga
     6301 gagaaatagt aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt tggaaatgtt
     6361 gagggcgagg atgtcttggc ctatcgattt gagacagacg gtggctacca acttgaggtt
     6421 atgacttatg gtgcgaccat cttgcgctat gtcgcacctg acaaggctgg aaattttgcc
     6481 aatgttatct tgggatttga tgactttgat agttatgtag gcaatagtcc caagcatgga
     6541 gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct caatggtaag
     6601 acctatgacc ttgaggttaa taatgctagc aactgtaatc acagtggttc aactggttgg
     6661 gattccagct tgtttgaagt tgaagaagta agcgatcatg gcttgactct ctacacagag
     6721 cgtacagatg ggacaggagg gttccctgga aatctcaaga tttggatcag ttatcacttg
     6781 gaagaaactg gtgcctatga aatcagctac aaggtaacga ccgatcagga tacgctggtc
     6841 aatccaacca accacagcta tttcaacttg tctggtgatt tcacgcagac gattgaccgt
     6901 catgtcttcc aactaaacac agagggcatt tactcaatcg ctcctgacgg tgttcctgcc
     6961 aaaactccag aagccaaccg tgatgtggtc aaacacatct acaatggtac cttgttgaag
     7021 gatatctttg cagaagaaga tgagcaaatc cagctggcat caggtttgga tcacccattt
     7081 gcccttcctg taggccatga caatgctgga ttcctttatg accaaaattc aggtcgcttc
     7141 ctgcttttca agacagaagc tccttgcttt gtggtctaca cagcaaactt tgtggatgaa
     7201 agtgtcatca taggaggtca gccaatgcta cagcacaatg ggattgctct tgaagcgcaa
     7261 gctttaccag atgccattca cagtgacctt aaaggccaag tcattcttaa agctggtcaa
     7321 accttcacca gtaagacacg ttatgaactt gttgtgaaat aaaaaagctg gtcaaaaagt
     7381 ctttaaaatt aaaataacgc atattgtcag gtgatgaaaa gtttgataat atgcgtttta
     7441 ttatgaaatg atttacttta tattatcctg aaatggtgtt atttttgagt tatctggctt
     7501 gactttcttg atggaaatta tataatagtt gtaataatta taattacaac gagaaaagag
     7561 gaccatttat gacctatgaa tataagagtc acatttattt ggcagagaca gttttaaatg
     7621 taaaggattt ggccagtcaa acagcctttt atcagcaagt tattggttta gaaatcttat
     7681 ctcaaactga gacagagtcc attctaggcc ttggtgaaaa agtcttggta cagctgattc
     7741 aagcacaaga gagcggagaa gtgagggaac attatggtct ttaccatttg gccattctct
     7801 tgcccacacg caaggctttg gctgatgtct tgaaacacct gacagattta cagattcctc
     7861 ttgtcggcgg tgcagaccat ggttacagtg aagctattta cttggaagat ttggagggaa
     7921 atggcattga actctatcga gataagccag tttccacatg ggatattcga gaagatggac
     7981 gcattatcgg agtgacggag gctcttgcgg cgcaggacat ttatgagttg ggggaaagag
     8041 tagagccctt tatcctggca gaaggtacga gaatggggca tattcatctt tccgtcaagg
     8101 atagtcgaaa gtccagccag ttttatcaaa aggtgttagg gctagaggat aaattcagtg
     8161 tgcctagcgc tagttggatt gcggctggag attaccatca tcatttagca gttaacgaat
     8221 ggggaggaaa aggtctggct tcgcgtaagc aaggcttacc aggtttagcc tactatgtca
     8281 tcgaagtcgc acataaaaaa gaactgttaa cgattgccca acgagcacaa gaagttgacg
     8341 caccaatcaa atggatgaca tcgagcaaat tggaaatcac agactcagat ggcatcgtga
     8401 cccgtattcg tttagcaagg taaaattagg aatttttaat aagttataag tattgctagt
     8461 ttgctttttg aacggtcttt ggtaacagaa ctgcgtgagc tggatgggtg agaattttga
     8521 caaagaagct gattagagat ataatgaaga aaaatcgagc aaggaaaaag aaatgacaaa
     8581 ttcaaagtat attactcgtt tgaaacgttc agagggccag ttgcgtggga ttcaaaagat
     8641 gattgaagga gatcgtgacg caactgacag cagtgagatc tagtgtagag cgcgtgattg
     8701 agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat gattctgaag
     8761 ctcaaaagga acgcttagaa aaggctatcc gatacttgat taaacggaaa taaatgaatg
     8821 ttttgagacg atatgtgcaa aggtctatta actattggaa tagtttaatg ggagtcaaaa
     8881 gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtatt gaaatgtact
     8941 gaccccaaaa agttagattt tttctgtcta acttttgggg tgcagttcat aagaaccttg
     9001 gtaatatgcg ttttattatg gggagattta tacttttcga aaatctcttc aaaccaggtc
     9061 agcttcacct tgccgtagat atggttactg acttcgtcag ttctatccac aacctcaaaa
     9121 cagtgttttg agctgacttc gtcagttcca tccacaacct taaaacagtg ttttgagcag
     9181 cctgcggcta acttcctagt ttgcacttta attttcattg agtattactt catcttctca
     9241 tgaaattgag tttttgccca gctcctcttg attattctac ttgaccaggc caagcattca
     9301 taccaccttc tacgttggta acggtgaggc cttgggagat gagaaattgg caggtagatg
     9361 cagagcggac tcctccttga caaataacat agtattcatg gtcaggtttg agttctttgt
     9421 agccttgctc taaggtactt aatggaagat ttttggcacc tggtgcatgt cctgcttgga
     9481 attcatgtgc ttcacggaca tcgataagtt ctagattttc attttggtat tttccataaa
     9541 agtcagccat gctgatacta gtttccatat tctactcctt ctgggttaac cattttgtat
     9601 agtgaataag cgccgtcaag gttttgggcg gtaaatcctg cttgcttgag gataggctct
     9661 gcgatatagc tgcgcaaacc actgtggcag ctgacgatgt aggcttgttt tttgtccagt
     9721 tcatcagctt cctccttgac actcggtcag attttgatac aatagtacaa aattagagga
     9781 ggcaggctat gattcagaaa catgcgattc ctattttaga gtttgatgac aatcctcagg
     9841 cggttatcat gcccaatcac gaggggctgg acttgcagtt gccaaagaag tgtgtttatg
     9901 catttttagg tgaggagatt gaccgctatg cgagggaagt aggggcgaac tgtgttggcg
     9961 aatttgtttc tgccaccaag acctatccag tctatgtcat caactacaag ggtgaggagg
    10021 tctgtctggc tcaggctcct gttggctccg ctccagcagc ccagtttatg gattggttgg
    10081 ttggctatgg tgtggagcag attatctcta ctgggacctg tggtgtccta gctgatatag
    10141 aggaaaatgc ctttctagtc cctgttcgcg ctctgcgaga tgaaggagcc agttaccact
    10201 atgtggcacc ttctcgttat atggaaatgc agccagaggc tattgctgct attgaggaag
    10261 ttttggaaga cagagggatt ccttatgaag aagtcatgac ctggacgaca gacggttttt
    10321 accgagaaac ggctgaaaag gtggcttatc gtaaggaaga aggctgtgct gttgtggaga
    10381 tggagtgttc cgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt
    10441 tgttcacagc agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg
    10501 aagcttttaa taaggcgcta gaactgagtt tagcaagtgt tcaccacctt tagttgtact
    10561 ggcaaaggat ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa
    10621 cgaggtcacc ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga
    10681 aaaacgtatc atatcaggtg atgaaaactt tgatactatg cgttttatgt tggtaagatt
    10741 tagagtgaga tgaaatgata ctcttcgaaa atctcttcaa accaggtcag cttcaccttg
    10801 ccgtatagat gttactgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc
    10861 tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagcaacct gtgactagct
    10921 ttctaagcga tgccttggtt ctcattgcct ataatcaaaa agagaaattt tctcctgaaa
    10981 agcatataga gtagctggcg ttaaaagctc ctgtcttgct tttttgacct atagtcacat
    11041 ctatcaagta ttgttcttgc ctaagctatc aataaaaagg tggcattttt taggcttggt
    11101 gttagtagat tttgccttat cctatctaag tcatttcgaa ctttttatgg tacaatggaa
    11161 acatgttatt caaattatct aaggaaaaaa tagagctagg cttatctcgt ttatcgccag
    11221 cccgtcgtat ttttttgagt tttgccttgg tcattttact aggctctctt cttttgagct
    11281 tgccctttgt ccaagttgaa agctcacgag cgacttattt tgatcatctt ttcactgctg
    11341 tctctgcagt ctgtgtgaca ggtctctcaa cccttccagt agctcacacc tataatatat
    11401 ggggccaaat aatctgtttg ctcttgattc agatcggtgg tctagggctc atgaccttta
    11461 ttggggtttt ctatatccag agcaagcaaa agcttagtct tcgtagccgt gcaactattc
    11521 aggatagttt tagttatgga gaaactcgat ctttgagaaa gtttgtctat tctatttttc
    11581 tcacgacctt tttggttgag agcttgggag ctattttgct tagttttcgc cttattcctc
    11641 aacttggctg gggacgtggt ctttttagtt ccatttttct agcgatctca gccttctgta
    11701 atgccggttt tgataattta gggagcacca gtttatttgc ttttcagacc gatttactgg
    11761 tcaatctggt gattgcaggc ttgattatta caggcggcct tggttttatg gtctggtttg
    11821 atttggctgg tcatgtagga agaaagaaaa aaggacgtct gcactttcat actaagcttg
    11881 tactattgtt gactataggt ttgttgttat ttggaacggc aactactctc tttcttgagt
    11941 ggaacaatgc tggaacgatt ggcaatctcc ctgttgccga taaggtttta gttagctttt
    12001 ttcaaacagt gacgatgcga acagctggct tttctacgat agattatact caggctcatc
    12061 ctgtgactct tttgatttat atcttacaga tgtttctagg tggggcacct ggaggaacag
    12121 ctgggggact caagattacg acattttttg tcctcttggt ctttgcacga agtgagcttc
    12181 taggcttgcc tcatgccaat gttgcgagac gaacgattgc gccgcgaacg gttcaaaaat
    12241 cctttagtgt ctttattatc tttttgatga gcttcttgat aggattgatt ctgctaggga
    12301 taacagccaa aggcaatcct ccctttatcc acctcatatt tgaaaccatt tcagctctta
    12361 gtacagttgg tgtaacggca aatctgactc ctgaccttgg gaaattggct ctcagtgtta
    12421 tcatgccact tatgtttatg ggacgaattg gtcccttgac cttgtttgtt agcttggcag
    12481 attaccatcc agaaaagaaa gatatgattc actatatgaa agcagatatt agtattggtt
    12541 aagaaaggaa agatcatgtc agatcgtacg attggaattt tgggcttggg aatttttggg
    12601 agcagtgtcc tagctgccct agccaagcag gatatgaata ttatcgctat tgatgaccac
    12661 gcagagcgca tcaatcagtt tgagccagtt ttggcgcgtg gagtgattgg tgacatcaca
    12721 gatgaagaat tattgagatc agcagggatt gatacctgcg ataccgttgt agtcgcgaca
    12781 ggtgaaaatc tggagtcgag tgtgcttgcg gttatgcact gtaagagttt gggggtaccg
    12841 actgttattg ctaaggtcaa aagtcagacc gctaagaaag tgctagaaaa gattggagct
    12901 gactcggtta tctcgccaga gtatgaaatg gggcagtctc tagcacagac cattcttttc
    12961 cataatagtg ttgatgtctt tcagttggat aaaaatgtgt ctatcgtgga gatgaaaatt
    13021 cctcagtctt gggcaggtca aagtctgagt aaattagacc tccgtggcaa atacaatctg
    13081 aatattttgg gtttccgaga gcaggaaaat tccccattgg atgttgaatt tggaccagat
    13141 gacctcttga aagcagatac ctatattttg gcagtcatca acaaccagta tttggatacc
    13201 ctagtagcat tgaattcgta aagagggatg acccctcttt tttgatgccc aagatggcaa
    13261 atagagacag aagccccttg tcttctagtt aaaagttctt caaaggctgg actttatgat
    13321 aaaatagaaa gaagtgacaa gagagagtaa tactcaatga aaatcaaaga tcaaactagg
    13381 aaactagctg cgggctgctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc
    13441 agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa catctatacg
    13501 gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtataagaaa aaatcagtcc
    13561 cctaaaggag cagattatga agttattgtc tatcgccatt tctagctata atgcagcagc
    13621 ctatcttcat tactgtgtgg agtcgctagt gattggtggt gagcaagttg agattttgat
    13681 tatcaatgac gggtctcagg atcagactca ggaaatcgct gagtgtttag ctagcaagta
    13741 tcctaacatc gttagagcca tctatcagga aaataaaggc catggcggtg tggtcaattg
    13801 cggtttggca gaggcttctg ggcgctattt taaagtagtt gacagtgatg actgggtgga
    13861 tcctcgtgcc tacttgaaaa ttcttgaaac cttgcaggaa cttgagagca agggtcaaga
    13921 ggtggatgcc tttgtgacca attttgtcta tgaaaaggaa ggtcagtctc gtaagaagag
    13981 tatgagttac gagtcagtct tgcctgttcg gcagattttt gactaggacc aggtcggaaa
    14041 tttctccaaa ggccagtata tcatgataca ctcgctgatt tatcggacag atttgttgcg
    14101 tgctagccag ttctaactgc ctgaacatac tttttatgtc gatgatctct ttgtctttac
    14161 gccccttcag caggtcaaga ccatgtacta tctgcctgtc gatttctatc gttatttgat
    14221 tgggcgtgag gaccagtctg tcaatgagca agtgatgatt aagcgcattg accagcaact
    14281 caaggtcaat cgactcttgg tagaccagct tgatttgtcc caagtgagtc atcctaaaat
    14341 gcgagaatat ctgctgaatc atattgaaat cacgacggtg atttccagta ccctgctcaa
    14401 ccgatctgaa acagcggagc atctggcaaa aaaacgccaa ttgtggacct atattcagca
    14461 gaaaaatcca gaagtctttc aggctattcg taagaccatg ttgagccgtt tgaccaaaca
    14521 ttctgtcttg ccagatcgca aactgtccaa tgtcgtctat caaatcacca aatctgttta
    14581 tggatttaat taatataagt gttttataag agggatttaa gaaaaatttt aactttttct
    14641 tagtcctttt taatttcagg agattatact agagtcatca aataaagaaa gactctaagg
    14701 agaatcctat gaaattcaat ccaaatcaaa gatatactcg ttggtctatt cgccgtctca
    14761 gtgtcggtgt tgcctcagtt gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa
    14821 gttctgtacg tgccgatgtg gtcaatccga ccccaggtca agtcttacct gaagagacat
    14881 cgggaacgaa agagggtgac ttatcagaaa aaccaggaga caccgttctc actcaagcga
    14941 aacctgaggg cgttactgga aatacgaatt cacttccgac acctacagaa agaactgaag
    15001 tgagcgagga aacaaactct tctagtctgg atacactttt tgaaaaagat gaagaagctc
    15061 aaaaaaatcc agagctaaca gatgtcttaa aagaaactgt agatacagct gatgtggatg
    15121 ggacacaagc aagtccagca gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa
    15181 atacaaaaga cagcatcgat gttcctgctg cttatcttga aaaagctgaa gggaaaggtc
    15241 ctttcactgc cggtgtaaac caagtaattc cttatgaact attcgctggt gatggtatgt
    15301 taactcgtct attactaaaa gcttcggata atgctccttg gtctgacaat ggtactgcta
    15361 aaaatcctgc tttacctcct cttgaaggat taacaaaagg gaaatacttc tatgaagtag
    15421 acttaaatgg caatactgtt ggtaaacaag gtcaagcttt aattgatcaa cttcgctcta
    15481 atggtactca aacttataaa gctactgtta aagtttacgg aaataaagac ggtaaagctg
    15541 acttgactaa tctagttgct actaaaaatg tagacatcaa catcaatgga ttagttgcta
    15601 aagaaactgt cgaaaaagct gttaaaga
//
LOCUS       DAVLTM010000043        15521 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLTM010000043 DAVLTM010000000
VERSION     DAVLTM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.043"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            83..541
                     /locus_tag="VYN16_001125"
     CDS             83..541
                     /locus_tag="VYN16_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1403664.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            554..1162
                     /locus_tag="VYN16_001126"
     CDS             554..1162
                     /locus_tag="VYN16_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403665.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1233..1565
                     /locus_tag="VYN16_001127"
     CDS             1233..1565
                     /locus_tag="VYN16_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403666.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1576..2991
                     /gene="ply"
                     /locus_tag="VYN16_001128"
     CDS             1576..2991
                     /gene="ply"
                     /locus_tag="VYN16_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1403667.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            3872..4588
                     /locus_tag="VYN16_001129"
     CDS             3872..4588
                     /locus_tag="VYN16_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1403668.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEALETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4712..5182
                     /locus_tag="VYN16_001130"
     CDS             4712..5182
                     /locus_tag="VYN16_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1403669.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQIQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            5199..6020
                     /locus_tag="VYN16_001131"
     CDS             5199..6020
                     /locus_tag="VYN16_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403670.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPA
                     SMMMGMPFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLM
                     RTTNDITQVQMLITMGLQVVTCGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            6027..7058
                     /locus_tag="VYN16_001132"
     CDS             6027..7058
                     /locus_tag="VYN16_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1403671.1"
                     /translation="MDFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGTGKTTIVNLLMKFYEIDKGSIHI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7124..7486
                     /locus_tag="VYN16_001133"
     CDS             7124..7486
                     /locus_tag="VYN16_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1403672.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7483..7986
                     /locus_tag="VYN16_001134"
     CDS             7483..7986
                     /locus_tag="VYN16_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1403673.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSAGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8100..8546
                     /locus_tag="VYN16_001135"
     CDS             8100..8546
                     /locus_tag="VYN16_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1403674.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8552..9226
                     /locus_tag="VYN16_001136"
     CDS             8552..9226
                     /locus_tag="VYN16_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403675.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVSLMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGTAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            9482..9670
                     /locus_tag="VYN16_001137"
                     /pseudo
     CDS             9482..9670
                     /locus_tag="VYN16_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            9920..10219
                     /locus_tag="VYN16_001138"
     CDS             9920..10219
                     /locus_tag="VYN16_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1403676.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10216..10533
                     /locus_tag="VYN16_001139"
     CDS             10216..10533
                     /locus_tag="VYN16_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1403677.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     SNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            10549..11175
                     /locus_tag="VYN16_001140"
     CDS             10549..11175
                     /locus_tag="VYN16_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1403678.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKDKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11208..11969
                     /locus_tag="VYN16_001141"
     CDS             11208..11969
                     /locus_tag="VYN16_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1403679.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12047..12442
                     /locus_tag="VYN16_001142"
     CDS             12047..12442
                     /locus_tag="VYN16_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1403680.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12712..12996
                     /gene="groES"
                     /locus_tag="VYN16_001143"
     CDS             12712..12996
                     /gene="groES"
                     /locus_tag="VYN16_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1403681.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13012..14634
                     /gene="groL"
                     /locus_tag="VYN16_001144"
     CDS             13012..14634
                     /gene="groL"
                     /locus_tag="VYN16_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1403682.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(14733..>15521)
                     /locus_tag="VYN16_001145"
     CDS             complement(14733..>15521)
                     /locus_tag="VYN16_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1403683.1"
                     /translation="SCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIR
                     DHFLKYDRAVRCRVKIITMDMFSPYYGLAKQLRFHIVQHLSRAMSRVRVQIMNQFHRK
                     SHEYKAIKRYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLY
                     QLLLFHFQNKEPEKFFGLIEDNLKQSHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLE
                     ATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         4616 a         2821 c         3583 g         4501 t
ORIGIN      
        1 gttatagtcc agtttttact atactttgat attcaaatat gttatactat ggttatcaaa
       61 gtaaatgaaa ggagttacga atatgacgcc agaagaaatg tacctgacag agcgattaga
      121 cgtacagata gctcattttt taaagaaaag cgttcaacat cgtagacgct ataaggtatt
      181 aaaaataaca gaaatcgtgg caggtttcct catagctgtc ttttgtgcta ttcctatgcc
      241 aggtgatcgc taccgtttga tttcggttgc cttatccagt ctcggcttgc tgtgtgaggg
      301 gattatcaat ttgtataatg caaaggaaaa ttggatttct taccaaaaaa ctgcgcaact
      361 cctggagaaa gaaaaattcc tctatcaatg ccaaacggag aaatatgcag gaaagaccaa
      421 ggcttttgcc ctatttgtca agacatgcga aggtcttatc tcagaggaga ttaaccagtg
      481 ggaaagtatc cagtcaaaag aagtggcagc tagtgcagat gctccagtta aaaaagagta
      541 ggaggtagag gaaatgtctc aatccagcta cctgtcgccc ttgctctggt taaaaaaaga
      601 agccgataag gaaaagatga gcgcgaccca gtgccagata tttttctttt actatcaaat
      661 gtttgagctc ttatttgcta gagaaagcga catgaaagac ttatgtctgg gaacgaaagg
      721 tttttatttc tcgcagttag agaaaaattt gctttctgga gtttcccgat ttctaaaaaa
      781 cttggagggg aaagtaactc tcaaggctaa ccaagaagta tcagctcgca aagccctttt
      841 tctagccttg acaactagcc aatcagattg gcaggagtta gctcctgttt ttgattttta
      901 tcagactatc gggaggcttg aaaatccttc tctcttgagt tctcaggaca gacaacatct
      961 gatgtggatt taccagtcag ctttggagaa ggattatatt gtcaaggtta ttggcgacaa
     1021 gcattttgta ttgaagagac aagatgctac taaattgaca gcgcgccaaa ctcaaacttt
     1081 ggaaattctg agtcaatcag aagacttggt caatcctgtc tatgttacat taggagaaaa
     1141 gggggtgctc ttgcttgatt aagagaggag atgttgtagc tctttatttg ccttttccga
     1201 ctattagtag cgatttggct gtgaagaatc atatgtatat ctgtattgac aacagcatga
     1261 ctaaaaacaa agagttggtt aaaaatcaga ccttcaaacc agctctcttg accagacgtt
     1321 tggtcaagaa ctttatgata gaagagccgg atctagctcg taatcctttt acaagaccaa
     1381 ccttgattga cttagataag gtatttatgt tggataatac ggttattccg acttcttatc
     1441 tagccagacg gcgacgcaat gtctcagaag aattgtacga ggaaattttg gatcacttag
     1501 tccaaccacg gctgatttcg ctgaacaagt ctgagtttat gcaactcaat ccaggaactt
     1561 attaggaggt agaagatggc aaataaagca gtaaatgact ttatactagc tatgaattac
     1621 gataaaaaga aactcttgac ccatcaggga gaaagtattg aaaatcgttt catcaaagag
     1681 ggtaatcagc tacccgatga gtttgttgtt atcgaaagaa agaagcggag cttgtcgaca
     1741 aatacaagtg atatttctgt aacagctacc aacgacagtc gcctctatcc tggagcactt
     1801 ctcgtagtgg atgagacctt gttagagaat aatcccactc ttcttgcggt cgatcgtgct
     1861 ccgatgactt atagtattga tttgcctggt ttggcaagta gcgatagctt tctccaagtg
     1921 gaagacccca gcaattcgag tgttcgcgga gcggtaaacg atttgttggc taagtggcat
     1981 caagattatg gtcaggtcaa taatgtccca gctagaatgc agtatgaaaa aatcacggct
     2041 cacagcatgg aacaactcaa ggtcaagttt ggttctgact ttgaaaagac agggaattct
     2101 cttgatattg attttaactc tgtccattca ggcgaaaagc agattcagat tgttaatttt
     2161 aagcagattt attatacagt cagcgtagat gctgttaaaa atccaggaga tgtgtttcaa
     2221 gatactgtaa cggtagagga tttaaaacag agaggaattt ctgcagagcg tcctttggtc
     2281 tatatttcga gtgttgctta tgggcgccaa gtctatctca agttggaaac cacgagtaag
     2341 agtgatgaag tagaggctgc ttttgaagct ttgataaaag gagtcaaggt agctcctcag
     2401 acagagtgga agcagatttt ggacaataca gaagtgaagg cggttatttt agggggcgac
     2461 ccaagttcgg gtgcccgagt tgtaacaggc aaggtggata tggtagagga cttgattcaa
     2521 gaaggcagtc gctttacagc agatcatcca ggcttgccga tttcctatac aacttctttt
     2581 ttacgtgaca atgtagttgc gacctttcaa aacagtacag actatgttga gactaaggtt
     2641 acagcttaca gaaacggaga tttactgctg gatcatagtg gtgcctatgt tgcccaatat
     2701 tatattactt gggatgaatt atcctatgat catcaaggta aggaagtctt gactcctaag
     2761 gcttgggaca gaaatgggca ggatttgacg gctcacttta ccactagtat tcctttaaaa
     2821 gggaatgttc gtaatctctc tgtcaaaatt agagagtgta ccgggcttgc ctgggaatgg
     2881 tggcgtacgg tttatgaaaa aaccgatttg ccactagtgc gtaagcggac gatttctatt
     2941 tggggaacaa ctctctatcc gcaggtagaa gataaggtag aaaatgacta ggagaagaga
     3001 atgcttgcga caaaaagagg cgatgatctc tctgcggata tctgaaagtt tatctcttgc
     3061 ctagcgattt ccattggagt atgctcgtga tgttaaaatg agtctatcaa acaggatgtg
     3121 taaaaaagta aaaagggtag ccaattcctt gatatacagg ggattgatta tcctttactt
     3181 gttttagatg attggaaatt caagaatgac acgtatcgga aatattttgc tgtttatatc
     3241 taatacccat ttatggaaaa aatgaaatta aatatgaaca aatcaattgc tagcaatggt
     3301 ttagaagtgc cagtgtacta gtctaacttc tccatcttgg gaacacgtaa attatactct
     3361 tcgaaaatct cttcaaacca ggtcagccct atccgcaact tcaaaacagt gttttgagca
     3421 ccctgcggct agctttctgg tttgttcttt gatttttatt gagtattaga ggtttgctta
     3481 gaaactcttc tattgatttc ttagaataca atagcgccta taagaactgt tggatttcaa
     3541 agtatttact tgaactatga tatgtctgga gtagactgtc aacaaaaggt gcagtatgat
     3601 tgtttttgtc gcttatatct acaaacttca aacagtaatt tgagctgact ttgtcaaact
     3661 tggctgaaac tttctatttg cagataatgg tctagtttgt tttttgaatg tggatgagtg
     3721 tctgttgata aagatagaaa ggcaagaccg tcttagcagg taaagtgcta tcctagcttg
     3781 atttgctaat aaaatccatt tcctctagtg aaaatcaaaa aaacttgtgt tataataaga
     3841 aagattaaaa tgtgaaaaaa ggagattcct aatgggacgt aaatgggcca atatcgtagc
     3901 caagaaaacg gctaaagatg gagccaactc taaagtatat gcaaaatttg gtgtagaaat
     3961 ctatgtagca gctaaaaaag gtgatccaga tccagaatca aactcagctt tgaaattcgt
     4021 tatcgaccgt gctaaacaag cccaagtgcc aaaacacatt atcgataaag cgattgataa
     4081 agccaaagga aacacagacg aaacctttac agaaggacgt tacgaaggtt ttgggccaaa
     4141 tggctctatg ctaattgtgg atactttgac ttctaacgtt aaccgtacag cagccaatgt
     4201 ccgtgcagcc tttggtaaaa acggcggaaa catgggcgct tcaggttctg tttcttacct
     4261 ttttgacaac aaaggtgtta ttgtatttgg aggtgaagat gcggacgcag tctttgagca
     4321 attgctcgaa gcggatgtgg atgtggatga cgtagaagca caagaaggta caatcacagt
     4381 ttacacagcg ccaactgacc ttcacaaggc tatcgttgcc ctccgtgagt ctggtattga
     4441 agaattccaa gtgactgaat tggaaatgat tcctcagtca gaagtggaat tgtcaggcga
     4501 agcccttgaa acctttgaaa aactttacag cgttcttgaa gacgacgaag acgtccaaaa
     4561 gatttatacg aacgtagatg ggttttaatt taacctatag aacatgatcc taagtgagaa
     4621 aatcacttgg gattttttta atcaagaaaa tagttctcat agaaatttaa ttattctcca
     4681 gagaactatt tttatttaga agggggagat tatggataaa ccgatgttag tctttaagcg
     4741 ttttggtcat caaattcacc tgatggtgca aaaggaagcc aaacgttgcg gcattgaatt
     4801 tatgggtggg ccgcaaggtc aggttgtgca ttttttggat aatcgcgaga aaaaccaaga
     4861 cttggtcttg attaaagata tcgagcagga actcaatatt accaagtctg ttgctagtaa
     4921 tctggttaag cgtatagtgc aaaatggttt ggtggaattg gaggcgagtc ctgttgataa
     4981 gcgggctaag tttgttcgtc tgacggacaa agcacgttct cagatacaac aggttaaggc
     5041 tttttttgaa cgcatagaca agcagttgat ggaagacatt gatgaagatg aattactgat
     5101 ttttgagaaa gttctcggtc aactacaggc aaatatcaag ggaataggag gagagaataa
     5161 agaaattagc caaaagaatt agtagaaaag aatgggggat gattttacta gccattctct
     5221 ttacttgctt ttcggtctat ctagagttgg aagtgccgac ctatatctcg aaaattacgg
     5281 atttgctagg tagtcaagga actaatttag atgagttgtg gcagccggca agcatgatga
     5341 tgggaatgcc ctttcttgcc ttcttgtccg tagttgcagt tggatttttt gcatcccgag
     5401 tggcggcttc ttatactagt aggctgagaa gtgatatttt taaccgagtt ttggattact
     5461 cgcagacaga gattaagaaa ttttcaattc ctagcctctt gatgcgtact accaatgaca
     5521 ttactcaagt tcaaatgttg attactatgg gcttgcaagt ggtaacgtgt ggtccaatta
     5581 tggctatctg ggctattggg aagattttag gtcattcaga atactggctc tgggccgtac
     5641 ttgtggcagt gattgtcaac gtcctgatga cgaccgtttt gatgacgcta gcctttccaa
     5701 aacagtcctt gattcagggg ctgacagata aactgaacag tatcactcgt gagagtttaa
     5761 caggtattcg tgtcgttcgt gcctacaatg cagaggatta tcaaaatgaa aaatttgcag
     5821 cagtaaatga tgaattgacc cgtttaaatt tgtttgtcaa ccgtcttatg gctattttga
     5881 atcctatcat gatggggatt tcaagtggtt tgagtgtggc gatttactgg attggggcct
     5941 atgtgattaa cgacgctgct ccgatagcgc gtctgcctct ctttagtgac atgattgttt
     6001 tcatgtctta tgccatgtag gttgtcatgg acttccttct catgggagca ctcttcatcg
     6061 ttcttccccg aactatggtc tctgctaagc ggattaatca agttttagat ttgcattctt
     6121 ctatccaaaa ccctgttcaa gtacagctga ctgatgaaaa cttcaaaggt caggtcgagt
     6181 ttaaggatgt gaccttccgc tatgcggcaa attcggaggc agttattgaa catgttagct
     6241 ttaaagcaga aactggtcaa acagtggcct ttattgggtc aacaggttct ggtaaatcaa
     6301 ctctggtcaa tctgattcca cgtttctacg acgtgtcagc aggagaaatt ctggtggacg
     6361 gtgtcaatgt tcaagactat gacttctctg cgacagctca tgctggtcaa aaggttgcca
     6421 ttgttgggcc gactgggact ggtaagacaa ccattgtcaa tcttttgatg aaattctatg
     6481 agattgataa gggaagtatt cacattgatg gtgtggatac caaggctatg acgcgttcag
     6541 aagtgcatga tgccttttca atggtcttgc aggatacctg gctctttgaa ggaactattc
     6601 gagacaatct catctataat caaataggga ttagtgatga acgaatgatg gaagctagta
     6661 aggctgtggg aattcaccac tttattatga ccttgccaga tggctatgat accatcttgg
     6721 atgacaccgt gaccttgtct gtaggacaaa aacaactatt gactattgct cgtgcccttc
     6781 ttaaggatgc accgcttttg attttggatg aggcgacttc ttctgttgac acacggacag
     6841 aggaattgat ccaaaaagcc atggaccgtt tgatggaagg acgcacatcc tttgtcattg
     6901 cccaccgctt gtcaaccatc cgaaatgcag acttgatctt ggtcatgaaa gatggaaata
     6961 tcatcgagca aggcaactat gaggaactga tggcgcaagg tggcttctac gctgacttgt
     7021 acaatagtca atttacagaa gatgaagcag aagaataaat caaaagaagg cttgacggcc
     7081 ttctttttct tatttatact agtacacata gaaagagaga agtatgagtc agaaattata
     7141 caatatgaaa tttgctgctg tctacttagc cttgattgca aaagttgagc gaaagggtgg
     7201 aaaggcagag tcagttcacc aagtgaccag ttggttgaca gggtatgaag tgagtgatgt
     7261 tcttgcttgt ctggataggg atgttactta cggagatttt tttagacaag caccatatta
     7321 tgtccctgaa cgaattgcaa ttacagggaa gatttgcggt gtccgtattg aggaaattga
     7381 tgatcctctg atgcaggaaa tacgtcgctt ggacaagcta gtggattggc ttgccaaggg
     7441 gaaaacatct caacaagttc tagaaaagta tgagaagcat aaatgaaaaa ttatcaagaa
     7501 tggtatcgaa atatcagctc cagactaact agtcgtccaa ttcttttatt cctattacgc
     7561 agtttcaatc gtttgatgac agtcgccatg cccctggtct atctgatctt gctagtcacc
     7621 acttacctgc aactaggact tggtcagcaa gttggggttt atgtgcttat tcctgcatca
     7681 ggttttgtgg ccttgtccct gtttcgtaag aaaatcaatc acccgagacc ttatgaaact
     7741 tgggatatct gtccccttct tgataaggat agtgcgggac ggtcgatgcc cagtcgccat
     7801 gtcttttcgg caactatcat ctccatggcc tgtctgcatg ctagtctacc tattggctta
     7861 gtatgcttga tcttttcagc tttgctgggg ttggtgaggg ttttaggggg tgttcattat
     7921 cccaaggatg tcttggttgg ctatgcttgt ggtttggtgt ggggattcct tttcttccta
     7981 ttctgacatg taagttaagc tagtcctaca accacttact gcttcaaatt gaccacttgc
     8041 ttttttgccc ttgtattcct aacttagctt tgatatagta gagtcagaaa ggagatgtga
     8101 tgaagttacg aattgagatt gacggcaatt tagaggagac tgaaattgtc atcaagaccc
     8161 ccactttgac agatgaaatt gcagacttgc aacggctctt gcaagagtca aaggctccga
     8221 ggttgacttt ttacaagggg acaggtgaat attatctaga cctgtcagaa attctcttct
     8281 ttgaaacaga agggagcaag atctacgctc ataaccagaa ggaagcttat gaggttcgcc
     8341 tcaagctcta tgagttggag tctatcttgc ctcgctattt taatcgagtt tccaagtcaa
     8401 cgatcgcaaa catccgtcag atttactcag tggacaagtc cttttcagga acgggcacca
     8461 tttcctttta tcagacgcac aaggaggttc atgtctcacg gcattaccaa tccctcctaa
     8521 aagaaaatct aagaaacatg aggtaaaaaa catgaaaaag aaagcatttg gtattgtgtt
     8581 acttgtacta gcagctttaa tattagtaca aggaaatttt ggaatccctt ctctaggagg
     8641 gcacatttgg cccttgattg gtattgcttt ttttgcctac caatcagttg gagctttgtt
     8701 gcgtcgccac ttaacttcag cctcttttac agctctagta tccttaatga ttgctaacca
     8761 cttttatggc attttaccga ttccaaacca gacactaatc tgggcaggag ttctaatcgt
     8821 tctcggagta aatatgttga ctcattctaa cagaacttgg aatgggaaaa aatggtggta
     8881 tgatggtgaa aaaaacattc ttatagataa ggaagttgct tttgggacag ggactttcta
     8941 caagcaagac caagaattgg tagaagacca agtagaagtt ggttttggca atgcaaaaat
     9001 ctactatgac aatgcagaga tgttaggtga ttttgcaact ttaaatattg aagtagcttt
     9061 tggtaatgca actgtctatg ttccgcaaca ctggcgtgtc gatttgaaag tagaaacatc
     9121 ctttggtaca gccaaggcgg acgctcctgt agcgccaaca agcaaaacct tgattattcg
     9181 tggagacgtg gcctttggga agttggagct tgtctacgtt aaataaaaaa atcttcactt
     9241 aaaccatcaa aatagacgta ctaagagtag gaaattgatg ccttgctctg atttcagttc
     9301 tatggttgtt agactttaaa aaatgaaatg ttgcctttaa aagttgtata tttttcgata
     9361 ttttgactct tacgtttgat gtatctatgt cctacagcgt ggatgatgta gtgtaaaatg
     9421 ctttaaaaaa aaaatgaatg atattggaca gtttttttga acttttaatt gctcaggagc
     9481 catgaaagca agcgcttggg tttatgacaa gggagaatgg tattatgtaa gttcttctgg
     9541 ttccatgatt gcgaatgatt gggtcaaaga caatggcaag tagtattatc tggcttcatc
     9601 aggtaagatg cttcgcaata cctacacacc tgatagttac tacgttggca acttaggtgc
     9661 ctggcaataa gaataatact cttcgaaaat ctcttcaaac cacgtcagct tcatccacaa
     9721 cctcaaaaca gtgttttgag ctgacttcgt cagttctatc tacaacctca aagcagtgct
     9781 ttgagcagcc tgcggctagc ttcctagttt ggtctttgat tttcattgag tataagaagt
     9841 ccttttcctt aaaggaagag gctttttact tgcttttttg ctgtttcctc atttgtccta
     9901 aagccttttt tgtgttaaaa tgaatggtat gaaagctaaa aaaatgtgga tggcaggtct
     9961 ggctctgctt ggtattggga gccttgccct tgctacgaaa aaagttgcag atgaccataa
    10021 gctcatgaag actcaggaag agttgacagc gattgtacga gaccattttt cagacatggg
    10081 ggaaattgcg accctctatg ttcaagtcta tgaaagcagt ctagagagct tgcttggtgg
    10141 cgttattttt gaggatggcc gtcattatac ctttgtctat gaaaatgaag acctagtcta
    10201 tgaggaggaa gtcttatgat acaaccagca agtttagaag aattggcatc tttagtagaa
    10261 aaagacggta agaaggtctt cctttttgtg gcggactggt gtggcgattg ccgttatatc
    10321 tatcctgcct taccagaaat tgaggagagt aatccagagt ttacctttat tcgagtggat
    10381 agagaccagt atatagattt ggccaaactc tgggatgtat atggaatccc tagccttgtt
    10441 gttctagaaa aggacaagga aatcggtcgt tttgtcaatc gcgaccgtaa aagcaagcaa
    10501 caaattaacg attttttagc aggattgaaa taggagaaaa aggaaacaat gatttttaca
    10561 tataacaaag aacatgtcgg tgatgtcctt atggtcatcg tgaaaaatag cggagatgct
    10621 aaactgaatg tggaacgcaa agacaaggta gcccgtgttt tcctcaaaga aaatggggaa
    10681 acagtagctt ggaacatttt tgaggtttcg agcttgtttg aaattgcaga gcgcggtcaa
    10741 gtctttttat cagatgagca agtcgctcgt ttgaaccaag aattacaggc ggaaggtttt
    10801 acagaagaaa ttgttaatga taaggaacct aagtttgttg ttggtgaaat tgtcgagatg
    10861 gtagctcatc cagacagtga ccacctcaac atctgccaag ttgcagtcgc aagtgacaag
    10921 atagtgcaaa tcgttgcagg agcacctaat gcgcgtgttg ggttgaaaac cattgtggct
    10981 cttcctggtg ccatgatgcc aaaagggaat ttcattttcc caggcgaact tcgtggtgaa
    11041 aagagttttg ggatgatgtg tagtcctcgt gaattgcatt tgccaaatgc tccgcaaaaa
    11101 cgtggggtgc ttgaattatc agaagaccaa gttgtcggaa caccgttcga cccagcgaaa
    11161 cactggactg cctaggaact tgtcagcatc tgatagaagg aaataagatg gcaaaaaatg
    11221 tagtgattac gggagcaacc tcaggaatcg gtgaagcgat tgcgcgtgct tatctggagc
    11281 agggtgagga tgtcgttcta acaggacgac ggatagacag attagaaatc ctcaagtcgg
    11341 agtttgcagt aagctttcca aatcaaaccg tctggacttt tccactagat gtgacggata
    11401 tggtcatggt gaagactgtt tgctctgata ttctagaaac gatagggagg attgatatct
    11461 tggtcaacaa cgccggactg gctcttgact tggctcccta tcaagactat gaggagttgg
    11521 atatgttgac catgttggat accaatgtta aaggtctgat ggcggttact cgctgtttct
    11581 tgccagcaat gataaaagtc aatcaaggtc atattatcaa tatggggtca accgcaggaa
    11641 tctacgccta tgctggtgcc gctgtttact cagctaccaa ggcagcggtt aagacctttt
    11701 cggatggact gcgaattgat accatcgcaa cggatatcaa ggtgacaacc attcagcctg
    11761 ggattgtcga aacagatttc tcaactgttc gttttcatgg tgataaagag cgggctgcgt
    11821 ccgtttacca aggaatagaa gccttgcaag ctcaggatat tgcagacaca gtagtctatg
    11881 tgaccagtca gcctcgccgt gttcagatta cagatatgac cattatggcc aatcaacagg
    11941 cgacaggttt catgattcat aaaaaataag aaatttcctc gaaaagttac aaatttctgt
    12001 aacttttttt gatttcctac gaatagataa gtaggaggaa gaaaatatgt ataataaagt
    12061 tatcatgatt gggcgtttaa cgtctacacc agaattgcac aaaaccaaca atgacaagtc
    12121 ggtagcgcga gcaactatcg ctgtgaaccg tcgttacaaa gaccaaaacg gtgaacgtga
    12181 agctgatttt gtcaatatgg tcctatgggg cagactagca gaaactttgg caagctacgc
    12241 aaccaaaggt agtctcattt ccgttgatgg agaattgcgt acccgtcgct ttgagaaaaa
    12301 tggccaaatg aactacgtga ccgaagtact tgtcacagga ttccaactct tggaaagtcg
    12361 tgctcaacgt gctatgcgtg aaaataatgc aggccaagat ttggcagatt tggtcttgga
    12421 agaggaagaa ttgccatttt aatactcttc gaaaatctct tcaaaccacg ttagctttat
    12481 ccacaacctc aaagcagtgc tttgagcagc ctgcggctag cttcctagtt tgctctttga
    12541 ttttcattga gtataaacat taaaaagtct gagttggtct caggcttttt atcttgagaa
    12601 agtcagactt ttttcttgac tatttctgac caagtgatac aatagaatta tgaattagca
    12661 ctcaagttca aagagtgcta ataatatcta tctcattatg gaggaaatca gatgttgaaa
    12721 ccattagggg accgtgtggt cttaaaaata gaagaaaaag aacaaaccgt tgggggcttt
    12781 gtccttgcag gctcagccca agaaaaaacc aaaacagctc aagttgtggc tactggacaa
    12841 ggtgttcgta ccttgaacgg tgacttggtt gctccaagtg ttaaaactgg agatcgtgtc
    12901 ttagttgaag cccacgcagg tcttgatgtc aaagatggcg atgaaaagta catcatcgta
    12961 ggcgaagcta acattttggc aatcattgag gaatagaagg agaaagtaag tatgtcaaaa
    13021 gaaattaaat tttcatcaga tgcccgttca gccatggttc gtggtgtcga tattcttgca
    13081 gacactgtta aagtaacctt gggaccaaaa ggtcgcaatg tcgttcttga aaagtcattc
    13141 ggttcaccct tgattaccaa tgacggtgtg accattgcca aagaaatcga attggaagac
    13201 cattttgaaa atatgggtgc taagttagta tcagaagtag cttctaaaac caatgatatc
    13261 gcaggtgacg ggactacgac tgcaacagtc ttgacccaag ctatcgtccg tgaaggaatc
    13321 aaaaacgtca cagcaggtgc aaatccaatc ggtattcgtc gtgggattga aacagcagtt
    13381 gccgcagcag ttgaagcttt gaaaaacaac gccatccctg ttgccaataa agaagctatc
    13441 gctcaagttg cagccgtatc ttctcgttct gaaaaagttg gtgagtacat ctctgaagca
    13501 atggaaaaag ttggcaaaga cggtgtcatc accatcgaag agtcacgtgg tatggaaaca
    13561 gagcttgaag tcgtagaagg aatgcagttt gaccgtggtt acctttcaca gtacatggtg
    13621 actgatagcg aaaaaatggt ggctgacctt gaaaatccgt acattttgat tacagacaag
    13681 aaaatttcca atatccaaga aatcttgcca cttttggaaa gcattctcca aagcaatcgt
    13741 ccactcttga ttattgcgga tgatgtggat ggcgaggctc ttccaactct tgttttgaac
    13801 aagattcgtg gaaccttcaa cgtagtagca gtcaaggcac ctggttttgg tgaccgtcgc
    13861 aaagccatgc ttgaagatat cgccatctta acaggcggaa cagttatcac agaagacctt
    13921 ggtcttgagt tgaaagatgc gacaattgaa gctcttggtc aagcagcgag agtgaccgtg
    13981 gacaaagata gcacggttat tgtagaaggt gcaggaaatc ctgaagcgat ttctaaccgt
    14041 gttgcggtta tcaagtctca aatcgaaact acaacttctg aatttgaccg tgaaaaattg
    14101 caagaacgct tggccaaatt gtcaggtggt gtagcggtta ttaaggtcgg agccgcaact
    14161 gaaactgagt tgaaagaaat gaaactccgc attgaagatg ccctcaacgc tactcgtgca
    14221 gctgttgaag aaggtattgt tgcaggtggt ggaacagctc ttgccaatgt gattccagct
    14281 gttgctacct tggaattgac aggagatgaa gcaacaggac gtaatattgt tctccgtgct
    14341 ttggaagaac ccgttcgtca aattgctcac aatgcaggat ttgaaggatc tatcgttatc
    14401 gatcgtttga aaaatgctga gcttggtata ggattcaacg cagcaactgg cgagtgggtt
    14461 aacatgattg atcaaggtat cattgatcca gttaaagtga gtcgttcagc cctacaaaat
    14521 gcagcatctg tagccagctt gattttgaca acagaagcag tcgtagccaa taaaccagaa
    14581 ccagtagccc cagctccagc aatggatcca agtatgatgg gcgggatgat gtaagctttc
    14641 tatagaaaac aacttataaa aaacacaaaa ggagggaatg actaaccctt ctttttatag
    14701 gctctttgtc aactgtagtg ggttgaagtc agctaagctc gagaaaggac aaattttgtc
    14761 ctttcttttt tgatattcag agcgatgaaa atccgttttt tgaagttttc aaagtttcga
    14821 aaaccaaagg cattgcgctt gataagtttg atgagattat tggtcgcttc cagtttggca
    14881 ttagaatagt gtagttgaag ggcgttgaca atcttttctt tatctttgag gaaggtttta
    14941 aagacagtct gaaaaatagg atgagactgc ttaagattgt cctcaataag tccgaaaaat
    15001 ttctctggtt ccttattctg gaagtgaaac agcaagagct gatagagctg atagtgatgt
    15061 ttcaagtctt ccgaatagct caaaagcttg tttaaaatct ctttattggt taaatgcata
    15121 cgaaaagtag gacgataaaa ttgcttatca ctcagtttac ggctatcctg ttgaatgagt
    15181 ttccagtagc gcttgatagc cttgtattca tgggattttc gatgaaactg attcatgatt
    15241 tggacacgca cacgactcat agcacggcta agatgttgta caatgtgaaa gcgaagctgt
    15301 ttagccaagc catagtaagg gctaaacata tccatagtaa taattttgac gcgacatcgg
    15361 acagctctat catatttaag aaagtgatct cggatgacag cttgtgttct tccctcaaga
    15421 acagtgatga tattgagatt gttaaaatct tgcgcaatga agctcatctt tccctttgta
    15481 aaagcatact catcccaaga cataatctca ggaagacaag a
//
LOCUS       DAVLTM010000044        14893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLTM010000044 DAVLTM010000000
VERSION     DAVLTM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.044"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(468..690)
                     /locus_tag="VYN16_001146"
                     /pseudo
     CDS             complement(468..690)
                     /locus_tag="VYN16_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(848..1102)
                     /locus_tag="VYN16_001147"
     CDS             complement(848..1102)
                     /locus_tag="VYN16_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1403684.1"
                     /translation="MGTKIGISKNTIGNYEKRVRSTKKNTIFDLVKVFSSLIDALFSP
                     VQKDSPSDIQSIYDQRAPPRQGKVLTYAERQLYDQKNEVS"
     gene            1538..2410
                     /locus_tag="VYN16_001148"
     CDS             1538..2410
                     /locus_tag="VYN16_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1403685.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(2559..2900)
                     /locus_tag="VYN16_001149"
                     /pseudo
     CDS             complement(2559..2900)
                     /locus_tag="VYN16_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3043..3354
                     /locus_tag="VYN16_001150"
     CDS             3043..3354
                     /locus_tag="VYN16_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403686.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            3358..4056
                     /locus_tag="VYN16_001151"
     CDS             3358..4056
                     /locus_tag="VYN16_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1403687.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALLDTIFLENFGHELYTQAPK
                     EIASLMNEWLAYFL"
     gene            complement(4185..5000)
                     /locus_tag="VYN16_001152"
     CDS             complement(4185..5000)
                     /locus_tag="VYN16_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1403688.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(4997..5902)
                     /locus_tag="VYN16_001153"
     CDS             complement(4997..5902)
                     /locus_tag="VYN16_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1403689.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKGFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            6359..8488
                     /locus_tag="VYN16_001154"
     CDS             6359..8488
                     /locus_tag="VYN16_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1403690.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNVYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            8475..8924
                     /locus_tag="VYN16_001155"
     CDS             8475..8924
                     /locus_tag="VYN16_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1403691.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            8983..9255
                     /locus_tag="VYN16_001156"
     CDS             8983..9255
                     /locus_tag="VYN16_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1403692.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(9609..9800)
                     /locus_tag="VYN16_001157"
     CDS             complement(9609..9800)
                     /locus_tag="VYN16_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403693.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNILSQQQK"
     gene            10228..10986
                     /locus_tag="VYN16_001158"
     CDS             10228..10986
                     /locus_tag="VYN16_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403694.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            10988..12976
                     /locus_tag="VYN16_001159"
     CDS             10988..12976
                     /locus_tag="VYN16_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403695.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTELTIFEKGQNRVQPKTIFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKTLILNDHQFSVKEEFNKDFIVKHVPNQF
                     NILTADYNYLVVPDLQAFLNQFPDSAIYNQFYGGMNVNVSEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(13018..13662)
                     /locus_tag="VYN16_001160"
     CDS             complement(13018..13662)
                     /locus_tag="VYN16_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1403696.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(13634..14683)
                     /locus_tag="VYN16_001161"
                     /pseudo
     CDS             complement(13634..14683)
                     /locus_tag="VYN16_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         4502 a         2775 c         3059 g         4557 t
ORIGIN      
        1 aaataagata tgcacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagcaa
       61 tcgtaatgta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
      121 aaaccttcaa agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg
      181 ttttagcgtt tttgtgttcc actctaacca ttatagcatt cttctcagct agtgtactaa
      241 ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag
      301 tatttgcctt ttgcaaagtg atgttaaatg cctttctcta aatttacata tcactattgt
      361 ttaacaaaat ctaatctatt ttaggtcact tattcttttt ttgaaatgta gaatgaactt
      421 tttcaaagtt tttcgaatct tttaaaatct gtttgtttta tatcgccatt ctccctatat
      481 agcctgacaa ctttcccgat ggtacgaata tggttgcttt cgtctaggtg gatgtcgggg
      541 tatttgggat tgagtttttt taaggtagcc ttggcggagt ttcttgacat agttagcgcc
      601 gtctacttgg aagatgccga tggtattata gtcaatctgt ggggtattct tgataaatag
      661 gtagtcgctg tttcttatct ttggctccat ggacttgctg acgacataag cgattgggtc
      721 gtagtcgtct gggataatgg aaactccata tctaaatcgt tgtcctgcat cgagcggcta
      781 cctgcagaga taaactacct aacacgagag taagtagtct gtctgtagtc gtccagtctg
      841 atgattttta cgatacttcg tttttctgat catacagttg cctctcggca taggtcagaa
      901 ctttaccttg tctgggtggt gcccgttggt cgtagataga ttggatatcg ctaggagaat
      961 ccttttgaac tggagaaaag agggcatcaa tcaagctact gaatacttta actaagtcaa
     1021 atatagtatt tttcttagta gacctaaccc ttttttcata atttccaatg gtgtttttac
     1081 ttataccaat cttagtaccc aattcttatt gagtccaacc attactagtc tatattgttt
     1141 tatagttgat tgagtttgga atagtacgct gtagctgcta aaacatttct aggaattaat
     1201 ttgcctttcc taatagagtt gttcatatct tatttcaatc tactatattt ttcgcctcta
     1261 acaatcgcaa tctcttcttt atctacgaat gaaatcgctt tctatttttg taagtaaagc
     1321 atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt ttcgtaaaaa atttgttgac
     1381 agagcacgaa acgcctacta taattaaatc aagcttaagg aaataacaaa aaaacaaatc
     1441 ggagggaaag acgatgaaca cattaaatga gaaagtaatc aatatctgta aagcagtagt
     1501 taaagaaact ttaatccaag acatttagga gaaagaaatg aagaaatcaa gaaaattagc
     1561 cacattagga atctgttcag ctttgttttt aggcttggct gcttgtcaac aacaacatgc
     1621 tacttctgag gggacgaatc aaaggcaaag cagttcagcg aaagttccat ggaaagcttc
     1681 atacaccaac ctaaacaacc aggtaagtac agaagaggtc aaatctctct tatcagctca
     1741 cttggatcca aatagtgttg atgcattttt taatctcgtt aatgactata ataccattgt
     1801 cggctcaact ggcttatcag gagatttcac ttcctttact cacaccgaat acgatgttga
     1861 gaaaatcagt catctctgga atcaaaagaa gggcgatttt gttgggacca actgccgtat
     1921 caatagttat tgtcttttga aaaattcagt caccattcca aagcttgaaa agaatgacca
     1981 gttgcttttc ctagataatg atgcgattga taaaggaaag gtctttgatt cacaagataa
     2041 ggaagagttt gatattctat tttcgagagt tccaactgag gcaactacag atgtcaaggt
     2101 tcacgctgaa aagatggaaa cattcttctc acaatttcaa ttcaatgaaa aagctcgaat
     2161 gctgtctgta gtcttgcacg acaatctgga tggcgagtat ctgtttgtag gccacgttgg
     2221 ggtcttagta cctgctgatg acggtttctt atttgtagag aaattgactt tcgaagagcc
     2281 ctaccaagcg attaaatttg ctagtaagga agattgctac aagtatttgg acaccaagta
     2341 tgcggattat acaggcgagg gactggctaa gccttttatc atggataatg ataagtgggt
     2401 taaactttaa tagtgttatg atatgacagt aatcttagga tttttatttc tatttttgat
     2461 gagttggtgg gttatttcaa tcgttaatag ccagaaacca cgcaaaaatg aaacatttat
     2521 tggctatgtc caaagatacg atattgatgg gaatggctat caatcattac cgtgtcctca
     2581 gaactgagag gagttcttga aatcgtaaaa ccactttgaa caagagttac ttcaacccat
     2641 tggctccgac ggattaagtt gctttcgtga ataccaaaat cagccgcaat ttcttcataa
     2701 gtgcggtatt ctcgcacata ttgaagagtg gccataagaa ggtcttctag gcttaattta
     2761 ggttttcgtc caccttttgc gtgtttaagt tgataagctg tttttaatac agctaacatc
     2821 tcttcaaaag tcgtgcgctg aacaccaaca agacgcttaa atcgtgcatc agttagttgt
     2881 ttacttgctt cataattcat agaactatta taccatgttt tgtttcgcag gaaatctaat
     2941 gctaaaatct tatcagtgag aagatttctt aaagtttatt ctttgtagtt tcatctgtat
     3001 tagtacataa tttaacttag agaaaatagg aggactaatc gaatggaatt aaaagatttt
     3061 acagaaaaag aacaggaaat gattaagaaa aggcttacaa tgtctaatat tagtgacaaa
     3121 gaaactactg agaagattct ggcgctagta ccacaagact tgattaagcg aatcccgttt
     3181 tttgtcagaa aacatgctac aacacgtacg attaaacgca tttcaattga acaccctgaa
     3241 ctctacgctg tagctcaaac aagtggtgag attccagaaa aagaacgcga agaattgcgt
     3301 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
     3361 aaaaaatatt ttattggcgg tttgggaaat aatgcctatc atagcaagga ttttcttcaa
     3421 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
     3481 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggc
     3541 cattctcttg gaggagattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
     3601 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
     3661 ttagaggaaa ctaaaaatta tatcaaatct caaattgttt cggacttagc tcttcttatt
     3721 tctaaagaga agtccgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
     3781 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
     3841 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
     3901 ttgtttatta gtcctcgcta tcctaatgga gctacatgga gagaggaagc cctacttgat
     3961 actatttttt tagaaaactt tggccatgag ctttatactc aagcacctaa agaaatcgct
     4021 agtctaatga atgagtggct cgcttatttt ctatgagcta cttgtattgc tggaaatggt
     4081 ataagatgaa aatctacaag tcactaattt tcatataaca aaaaacgaac aggctctttt
     4141 tctgttattc agaagagaac ttgttcgttt ttatcttgat tttcttaaaa agctctatat
     4201 acatagggat tttctggttt atcgactttg gtaaagctgt cgatttccaa ggttggaagg
     4261 ttttgtttga gttctttatg gtagtgattg accagttttc ctttggctgt tatccaagtg
     4321 ttgttttcat actgccgggt attgcccttg gtcagcaatc catagacacc agaatctgcg
     4381 atacagtgga taatgccgaa gcggaacaga aattggctat tggcatgact ggggtcgtta
     4441 tagacaaagc ctgtaaactg gattgtcttg ccctcaaact catctggata gtcgtagata
     4501 gcctccatga cttccatata gttttcatta gtgatctgaa tactatcttg ggataagtat
     4561 ttatccgccg ccgttcgcat ttccttttca taggctgatt ttgaaaaata agaactggta
     4621 tctggtttca aatattggct tgtcgtccct tcgcttgtct gaatggctag atccgttcct
     4681 tctgataggg ggaaatgata acctttagca gaaacggtct gagaatccaa gctaacagtt
     4741 gggaaagtta agccgatgac aagtggaata gccagaagag aaatactcgt catcttggct
     4801 aatcggctgt tcagatgact gtgggttttg acttgcttca tccagatata caattgaaca
     4861 atagccaaga taaaggaaag caccatggaa atataggcca gataggaata gtgcatgttg
     4921 atgtactggt ttagtttgcc cgacagatgg aggtaaatag tcagttcaaa atagccagct
     4981 aaaactaaaa atcgaatcat aggatgactc caatcaagag agaataggct aaaacgacca
     5041 gagtcacaat cgtaatgaag tgactaataa atcgtgcttt caagtaattt ttcatcatga
     5101 gaatattttt gatatccagc attgggccaa tgacgagaaa ggccagaact ggagccaaac
     5161 cgaaactcga gagtagagaa gcacctataa aggcatccgc ctcactacag agcgaaagaa
     5221 gaaaggccaa aagcatcaag agcaggatgg caaaaagagg agtcgcactg atagaggtca
     5281 gaatccgagt cggaacgtag acctgtatta tagaagcaaa gaggcagcca aataccaaat
     5341 aacgccccgt atcaaaaaat tcatcaatgg cctgcacaaa gacttgaaaa cctttttttg
     5401 cagaactcaa gtaagaaaaa tcatgctcat gacaagccag acgattttct ttctgaatcg
     5461 gttcttgcca gaaaaatcct agaaaaattc ctagtattac agccacaaga atggaaccca
     5521 gagctcgtaa tagggcgaca tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa
     5581 caatgggatt gataacaggt gctgtcacaa gaaaaggaac ggccgtgtaa cttggaacct
     5641 ttttttccag aaaacgattg atgatgggga cgattccaca ttcacaggaa gggaaaagta
     5701 taccgacaaa ggtcccaaaa aagattctcc cccaacgatt tcgagggaga aaatgataaa
     5761 ccttgtcagg tgtgatataa acttcaatca gccctgagac aatgcttcct atcagaacga
     5821 agggaagggc ttcaatgata atggagagaa aaatggctcc agtttgaagt acactagatg
     5881 ggagttgttg aaaagcagtc atctagtttt tcttttctgc tttggctttt ttctctttgt
     5941 catcagggaa agtgacttgt ttcaaatcag ggagtttagc aaactcttca aacatcttgt
     6001 ccaagtcgtc tcgttttgta aatttgacag ccataggtgt acctcgattc taaattctac
     6061 ctctattata ctattatttt gcggaaaagg gagtttttta atttgttttc gatagttgat
     6121 actcttcgaa aatctcttca aaccacgtca gcttcgcctt accgtagtac ggttactgac
     6181 ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt cagttctatc
     6241 cacaacctta aaacagtatt ttgagcagcc tacggctagc ttcctagttt gctttttgat
     6301 ttttattgag tatgaaaagg attggagaaa gctttccagc ttgtggtata atagatttat
     6361 ggataaaaaa tatgaaaaaa tctctcagga tttgggagtg acgttaaagc aaattgatac
     6421 cgttctaagt ttgacagctg aaggggcgac tattcccttt atcgcgcgtt atcgcaagga
     6481 catgactggt agtctggatg aggtggcgat taaggctatt attgatttgg ataaaagtct
     6541 gacaaatctc aatgaccgta aggaagctgt cttagctaag attcaagaac aaggtaagtt
     6601 gaccaaggaa ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg ttgaagaact
     6661 ctatcttcct tataaggaaa agcgtcgtac caaggcaacc attgcccgtg aagctggact
     6721 ctttcctctt gctcgcttga ttttgcagaa tatagttgac ttagagaaag aagctgaaaa
     6781 gttcgtctgt gaaggatttg cgactggcaa ggaagccttg accggtgcag ttgatatttt
     6841 ggtcgaagcc ttatcggaag atgtgacctt gcgttctatg acttatcagg aagtgctgag
     6901 acactctaaa cttacttctc aagccaagga tgaaagtctt gatgaaaagc aggtttttca
     6961 gatttattat gatttttcag agacagttgg aactatgcaa ggctatcgta ccttggctct
     7021 caatcgtggg gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga cggaccgtat
     7081 tcttgccttc tttgctactc gtttcaaggt gaaaaatgtt tatattgatg aagttgttca
     7141 gcaatccgtt aagaaaaagg tcttgcctgc tatcgagcgt cgtattcgga cagaattaac
     7201 tgagaaagct gaagaaggag ctatccaact tttttctgac aatctgcgca atctcctctt
     7261 ggttgctcca ctgaaagggc gcgtggttct tggatttgac ccagcctttc gtacaggtgc
     7321 caagttagct gtcgtggatg caacaggaaa aatgctgaca actcaggtta tttatcctgt
     7381 taaaccagca tcagctcgtc aaatcgaaga agccaagaaa gatttagcag atttaattgg
     7441 tcaatacggt gtagagatta ttgccattgg aaatggaacg gccagtcgtg aaagtgaagc
     7501 ttttgtagcg gaagttctga aagatttccc tgaagtcagc tatgttatcg ttaatgaaag
     7561 tggtgcttct gtctattctg ccagcgaact tgctcgtcag gagtttccag acttgaccgt
     7621 tgaaaaacgt tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg cggaattggt
     7681 caaaatcgat cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtca gtcagaagaa
     7741 actatctgag agtctggact ttgttgtcga tacagtggtt aaccaagttg gtgtcaatgt
     7801 caatacagct agcccagctc ttctttcaca cgtagctgga ctcaacaaaa ctatctctga
     7861 aaatattgtc aaataccgcg aggaagaagg aaaaatcact tcacgcgccc aaatcaagaa
     7921 agttcctcgt ctgggagcca aggcctttga gcaggctgct ggtttccttc gtatccctga
     7981 aagtagcaat atccttgata atacaggagt tcacccagag aattacactg cagttaagga
     8041 actcttcaaa cgtttagata ttaaagacct aaacgaagaa gcccaaagca aactcaagtc
     8101 cctttcagtc aaggaaatgg ctcaagagct agacctcggt ccagaaaccc ttaaagatat
     8161 cattgcagat cttctcaaac caggtcgaga tttccgtgat tcctttgacg cacctgtgct
     8221 tcgccaagat gtcctagata tcaaagacct agtggtaggt cagaagctag aaggtgttgt
     8281 gcgtaacgtt gttgacttcg gtgccttcgt tgatatcggg attcacgagg acggcttgat
     8341 tcatatttct catatgagtc gcaagtttat caaacaccct agtcaagtgg tgtcagttgg
     8401 agatttggtg accgtttggg tcaagaaaat tgatactgaa cgtgaaaaag ttaatctgtc
     8461 gctcctcgct ccaaatgaaa ctgactgatt atgtcaagca ggtttcacta gaagacttcg
     8521 ggagagcttt tatccatcat gtccagtgga ataggcgtct acgttcgaca ggtgggcgat
     8581 ttttccccaa agatgggcat ctggatttta atcccaaggt ttatcagaaa ctgggtatgg
     8641 aggtttttag gaaaattgtc cgacatgaac tctgtcatta tcacctttat ttccaaggga
     8701 agggctatca acacaaggat cgggatttta aggaactttt gaaagcagtg gatggattac
     8761 ggtttgtacc atccttgccc aatagtaact ctaaaccact caagctttat cgttgtcaat
     8821 cctgccagca aaggtatcag cgcaagcgta ggattgatac caaacgctat cgctgtggac
     8881 tttgtcgagg taaattgctt ctgataaatc agcctgagga ctgatgaaag ccgagcctcc
     8941 ccgtgatata ctatgtctaa cctagcagaa agaggaaata tcatgaacag taaattttat
     9001 aaaatgagac gcaatcgcat gatttcagga gttctggcag gtctgtcaga caagtggaat
     9061 tttgatgtta ccctagttcg tttcttgttt gctattttta cagttgcaaa ctttggactt
     9121 ggcgtgatta tctatatcat cctagcttct atcatgccta ccaaggaaga aatcgaagca
     9181 gaaatgtacg gaacaggccc acgtaaacgc aaagaagccc aagccattga cgacaatgaa
     9241 ggctggtttt ggtgaggttc tacagaagat ttggaataag caaaaagctt taaatcaata
     9301 gtctaaactg cttgatttaa agctttttta cttttatact caataaaaat caaagtgcaa
     9361 actaggaagc cagccacagg ctgtaattta gtcggcaatg tgaagttgac atagtttgca
     9421 ctttgatttt tgaatagtat taataatatt tcgccagctt catccacaaa ctatagtgga
     9481 ttggaactag aatagtacac ctctacttct aaaacattgt tagaaatcga tttgactatc
     9541 ctgatcgatt tgtcctgttc ttatttcatt ttattataaa agcagtaatt taaacgcagt
     9601 tttttaatct atttttgctg ctgagacaat atatttttgg aaaaatggtg gatgctgata
     9661 acaactgata accagcaaat aacataagca gtgccggtaa attctgtacc gataaagtgt
     9721 gtgtgttcaa ctaagagctg accgatgaat ggagagagca gatagagaag tccgatgagt
     9781 atatgttctt tgtgtttcat taaagcgcac cttctttcct tataggttga acgacttgca
     9841 agtgttaaat tgaatggtat gttatctagg taaccttact aaaatgttag ttcccgcttc
     9901 ctccttttca aagtaagcct tttcacactt atattttaca actttaaaaa taggaaatca
     9961 aatcattttc taagtaattt tgcttatatt tcacccaatc ttacaaaaat gtaagattaa
    10021 agtctctttt gtaaggttag gttatggcaa gcggtctttt tttgatgtaa actagactca
    10081 taaagaacaa aggagtaaca tcatgaaaca aatcttacat aagaaaacta gaaaaccaag
    10141 tcacaaagaa attgagcgcg ttcagctggg atgcactatg atgcaagcta catttcattt
    10201 gatgggatat taagaaagga gattctcatg acacttttag atgtaaaaca cgttcaaaaa
    10261 atttataaaa cacgttttca gggcaaccaa gtagaagccc tcaaggatat tcactttacc
    10321 gtcgaaaagg gtgactacgt tgccatcatg ggtgagtctg gttctggtaa atcaactctt
    10381 ctcaatattc tagctatgct ggataaacca agtcgtggtc aggtttactt gaatggaact
    10441 gacaccgcaa ccattaaaaa ttcacaggcc tctagcttcc gtcgtgaaaa gttgggcttt
    10501 gtcttccaag actttaactt gctagatact ctatctgtaa aggacaatat cttgcttccg
    10561 cttgtcttgt caagaagacc tattacagag atgatgaaga aattggtggt gacagctgag
    10621 aatctgggaa taaaccaatt acaagagaag tacccttacg agatttctgg tggtcagaaa
    10681 cagcgtgtag cagtagcccg cgccatcatc acagaacctg aaattctcct tgcggacgaa
    10741 ccgacaggag ctcttgactc caagtcatct gcagccttac ttgatgtctt tgatgaaatc
    10801 aatgagcgcg gccaaaccat tctcatggta acccactcaa cagcagcagc tagcagagct
    10861 aaacgtgtac tctttatcaa agacggcatt ctttacaatc aaatctaccg tggagagaag
    10921 acagaacgtc agatgttcca agaaatctct gataccttga ctgtcatggc aagcgaggtg
    10981 aattagtatg tttcgattaa ccaataagtt agcggtatcg aacttgatta aaaaccgcaa
    11041 actctactat ccttttgcgc tggctgttct cttggcagtc actgtcacct atctctttta
    11101 ctctctaacc ttcaatccta agattgcgga aatccgtgga ggaacaacca ttcaggctac
    11161 acttggattt ggtatgtttg tcgtcaccct tgcgtcagcc attatcgttc tctatgccaa
    11221 tagttttgtc atgaagaacc gttccaagga actaggaatt tatggcatgt tgggcttgga
    11281 gaagcgtcat cttatcagta tgacctttaa ggagttagtg gtatttggga ttctaactgt
    11341 tggagcgggt atcggtattg gagccttgtt tgacaagtta attttcgctt tcctgctcaa
    11401 actaatgaaa ttgaaggttg agctggttgc taccttccag acgaaagttg tcattacagt
    11461 gcttgttgtc ttcggtttga ttttcctagg cctcatgttc ctgaatgccc ttcgaatcgc
    11521 ccgtatgaat gccctccagc tctctcgtga gaaagctagt ggagagaaaa aaggtcgctt
    11581 ccttcctctc caaaccattc ttggttccat aagtttagga attggctatt atcttgccct
    11641 tacggtaaaa gatcctctta cagccttaac aaccttcttc atagctgttt tactggttat
    11701 ctttgggact tatctcttgt ttaatgcagg gattaccgtt ttcctccaaa tcttaaagaa
    11761 aaataagaaa tactattacc aaccaaataa cctcatatct gtttctaact tgattttccg
    11821 tatgaagaaa aatgcagttg gactagccac catcgctatc ttgtcaacaa tggttttggt
    11881 aaccatgtca gcagcgacaa gcattttcaa ttcctcagaa agctttaaaa aagttctaaa
    11941 tcctcatgat tttggggttt cagggcaaaa tgttgaaaaa gaagacttgg acaaactctt
    12001 gagccagttt gcaagtgaca aaggttataa gattaaagaa aaagaagtat ttcgttacac
    12061 ttactttgct gttgcgaatc aagaaggaac cgagttaact atttttgaaa aaggacaaaa
    12121 ccgtgtccaa ccaaaaacaa ttttcatggt atttgaccaa aaagattatg aaaatatgac
    12181 tggtcaaaaa ctgtctctat caggaaatga ggtcggactc tttgcaaaga atgagggagt
    12241 taaagaacag aaaactctaa ttctgaatga tcatcaattt tctgtaaaag aagaatttaa
    12301 taaagatttt attgtcaagc atgtcccaaa tcagtttaat attttgactg ctgattacaa
    12361 ttaccttgtt gtacctgatt tacaagcctt tttgaaccaa ttcccagatt cggctatcta
    12421 taatcagttt tacggtggta tgaatgtaaa tgtcagtgaa gaagagcaac tcaaagtcgc
    12481 tgaagaatat gaaaaatact tacaaaagtt taatgctcaa ttaaacactg aaggtaacta
    12541 tgtgtatggt agcactctag cagatgctag tgctcaaatg agtgtcctct ttggcggtgt
    12601 cttctttatc ggtattttcc tatccattat ctttatggtc ggaaccgttc tggtcatcta
    12661 ctacaaacaa atttctgaag gctacgaaga ccgtgagcgc tttattatct tgcagaaagt
    12721 cggtttagat caaaagcaaa tcaagcaaac catcaacaaa caggttttaa ctgtattctt
    12781 ccttcctttg ctctttgcct tcctacatct agcctttgcc taccatatgc ttagtctgat
    12841 tttaaaagtg attggtgtac tggatacgac tatgatgttg attgtgacct tgtctatctg
    12901 cgctatcttc ctcatcgcct atgtgctgat tttcatgatt acttcaagaa gttatcgcaa
    12961 gattgtgcaa atgtaaaaaa gatacctcga cttcaaaatc gaggtatttc ttgttttcta
    13021 aatgctgaaa agttgtccga gcaggaaggt aactcccata gttaagagac caatagcaag
    13081 gttccgaatc atagctgttt tggttggggc ttttccaagt ctagcgcttg tgtagccagt
    13141 gagaagaagg gctacaccga cgataaggac ggtagcaggg atgcggtaat cacttggaaa
    13201 aatggtcact gacagcattg gaggcaaact tctaaggaaa aaggcaacga agctagaaat
    13261 ggcagcgtgc caaggattgg taaattcttc atactcaatc ccatattttt cctctaccag
    13321 agccttgagt ggatttttaa gaaagatctt attggtcaag agttgggcag aagttttgca
    13381 ttctccattt tggatataag cagcatagag ggattttttg gctagttccc tatcttggtc
    13441 taacaagagt ttttctcgcg aaacggcagc ttcctcggta tcttttggag ttgaaacgga
    13501 tacatattct ccaccagcca ttgaaaaggc accagctaag atagccgcaa atcctgataa
    13561 aaagataatc cagatattgg tcgtggcact ggcaaccccg ataaccacac cagcaatgga
    13621 aataattcca tcgttagcac caagaacacc tgcgcgcagg atatttaaac gacctgcaaa
    13681 atttgaatcg atttcgtgat ttgtttctga cgctaaattt caagttcaag ttagccatcc
    13741 agaagtcttc tctgggtgac ttgtagtcca agcatttttt aggatagttg ttaatccact
    13801 tttcgatgaa tgcgacttcc ttaggagtca ttttcttggt tcccttaggt aaccatctac
    13861 gaatgaacct gttgtgattc tcattagttc ccctttccca agaggcatag gggtgcgcat
    13921 aataaatgtg ctcctcagaa aatacatcag acaagcgatt gaattccgtt ccattatctg
    13981 ccgtgatgga aagaatcttg tgttgtttta agatgagttt tagagcctga ttgaccacct
    14041 cagcactttt atttggaatc aatcggatga tctgatgtct actctttcga tccgtcaaga
    14101 caagcaagca gtagtttttc gatctcgtaa gtagaaccgt atcaatctca taatgcccat
    14161 tctccaagcg aagattgata gcttcaggcc gctgtgcgat ggattgacca gcaggtttaa
    14221 agttggtgct agcctgtttc ttaagcactt ttccttttct agggtaaagc agatcctgct
    14281 tgcttaaccc caattttcca tgatgaatcc aatagtaaat ggttgaaatt cccacgttaa
    14341 cccctttagc catcaccatc atttcaggcg aaaatttttg gttatgatag tggagaatct
    14401 tttcctttag ttcctttgtc aagcttgatt tcttgaccga gcgcttgcga ttgttttcat
    14461 aagactgttg agcatagtcg gcagaataaa cctctttgaa gcgccctttt ccaagacatt
    14521 gtcggactgt cccacgcttg atttcagtgt ggatagttta aggagctttt ccaagtagag
    14581 aggcaatttc tctatttgat ttcccttctt ttttccatct ttcgattaag cgacggctat
    14641 cgattgtcaa atgtttggct tttgtagtat aattttcttg catctctgtg cctttcttgt
    14701 gtttgtggtt gaacaacaag tataacacag aggtgttttc ttatgcctac aagagctatc
    14761 agctagttga accatctaat ttttaggagg gctgggtggc taacttcatt atagaacttt
    14821 catttacgaa catatagtaa aatgaaacaa gaacagaaca aatcgatcag gacagtaaaa
    14881 tctatttcta ata
//
LOCUS       DAVLTM010000045        14768 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLTM010000045 DAVLTM010000000
VERSION     DAVLTM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.046"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            224..1288
                     /gene="pepA"
                     /locus_tag="VYN16_001162"
     CDS             224..1288
                     /gene="pepA"
                     /locus_tag="VYN16_001162"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1403697.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            1310..1606
                     /locus_tag="VYN16_001163"
     CDS             1310..1606
                     /locus_tag="VYN16_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1403698.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            2288..2731
                     /locus_tag="VYN16_001164"
     CDS             2288..2731
                     /locus_tag="VYN16_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1403699.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            2735..3499
                     /locus_tag="VYN16_001165"
     CDS             2735..3499
                     /locus_tag="VYN16_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1403700.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            3514..4242
                     /locus_tag="VYN16_001166"
     CDS             3514..4242
                     /locus_tag="VYN16_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403701.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            4235..5755
                     /locus_tag="VYN16_001167"
     CDS             4235..5755
                     /locus_tag="VYN16_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1403702.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            6100..6906
                     /locus_tag="VYN16_001168"
     CDS             6100..6906
                     /locus_tag="VYN16_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1403703.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(7073..7609)
                     /locus_tag="VYN16_001169"
     CDS             complement(7073..7609)
                     /locus_tag="VYN16_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1403704.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIINLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            7742..8632
                     /locus_tag="VYN16_001170"
     CDS             7742..8632
                     /locus_tag="VYN16_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1403705.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            8876..9229
                     /locus_tag="VYN16_001171"
     CDS             8876..9229
                     /locus_tag="VYN16_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1403706.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            9230..10267
                     /locus_tag="VYN16_001172"
     CDS             9230..10267
                     /locus_tag="VYN16_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1403707.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAVTTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(10313..11320)
                     /gene="galR"
                     /locus_tag="VYN16_001173"
     CDS             complement(10313..11320)
                     /gene="galR"
                     /locus_tag="VYN16_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1403708.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            11485..12663
                     /locus_tag="VYN16_001174"
     CDS             11485..12663
                     /locus_tag="VYN16_001174"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1403709.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            12682..14163
                     /locus_tag="VYN16_001175"
     CDS             12682..14163
                     /locus_tag="VYN16_001175"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1403710.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQAAFMRFVEQVGISLD"
     gene            14233..14637
                     /locus_tag="VYN16_001176"
     CDS             14233..14637
                     /locus_tag="VYN16_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1403711.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGRRSEDRQVRI"
BASE COUNT         4228 a         2632 c         3561 g         4347 t
ORIGIN      
        1 tttcatgaaa aatccaaaca actttgatgg tgtattgttt tttgtttgga aataaatatt
       61 cgagttaatt ttggaatgtt tactagattt tgcactacat gcaagagaat actatccttt
      121 tctaagatag agcaggacta agatactaac ttagaaatcc accttcccac tatccttctc
      181 ctataaaaag tggtaaaatg gatgaaagaa agaaggaact gaaatgacaa cattattttc
      241 aaaaattaaa gaagtaacag aacttgctgc agtctcaggt catgaagcgc ctgtccgtgc
      301 ttatcttcgt gaaaagttga caccgcatgt ggatgaagtg gtgacagatg gcttgggtgg
      361 tatttttggt atcaaacatt cagaagctgt ggatgcaccg cgcgtcttgg tcgcttctca
      421 tatggacgaa gttggtttta tggtcagcga aatcaagcca gatggtacct tccgtgtcgt
      481 agaaatcggt ggctggaacc ccatggtggt tagcagccaa cgtttcaaac tcttgactcg
      541 tgatggtcat gaaattcctg tgatttcagg ttctgttcct ccgcatttga ctcgtggaaa
      601 ggggggacca accatgccag ccattgccga tatcgttttt gatggtggtt ttgcggacaa
      661 ggctgaggca gaaagttttg gcatccgtcc tggtgatacc atcgtaccag atagttctgc
      721 aattttgaca gccaatgaaa aaaatatcat ctcaaaagct tgggataacc gctacggtgt
      781 cctcatggta agcgagctag ctgaagcttt atcaggtcaa aaactcggca atgaactcta
      841 tctgggttct aacgtccaag aagaagttgg tctgcgtggc gctcatacct ctacaaccaa
      901 gtttgaccca gaagtcttcc tagcagttga ttgctcccca gcaggtgatg tctacggtgg
      961 tcaaggcaag attggagatg gaaccttgat tcgtttctat gatccaggtc acttgcttct
     1021 cccagggatg aaggatttcc ttttgacaac ggctgaagaa gctggtatca agtaccaata
     1081 ctactgtggt aaaggcggaa cagatgcagg tgcagctcat ctgaaaaatg gtggtgtccc
     1141 atcaacaact atcggtgtct gcgctcgtta tatccattct caccaaaccc tctatgcaat
     1201 ggatgacttc ctagaagcgc aagctttctt acaagccttg gtgaagaaat tggatcgttc
     1261 aacggttgat ttgattaaac attattaaac cataagggag gtggtaggga tggcagaacc
     1321 aaacctagaa agccttataa aagatcttta caatcatgct cgacatgatt tgagtgaaga
     1381 tttagttgct gctctcctag agactactaa aaaactgcct actacaaatg agcaattgca
     1441 ggcagttcgt ctctcaggcc tggtcaatcg tgaattgctc ctaaatccca aacatccagc
     1501 acctgagttg ctcaacttgg ctcgctttat caaaagagaa gaagccaagt acagaggaac
     1561 tgcgacttct gcacttatgt atgaggaact ttttaaaatg ctttgattcc attgtggatc
     1621 aaagcatttt ttataagacc tatctcgtga gcgttcaaat agtagtaaca aggaatcatg
     1681 agaagtcagc cgagaccata gtagtgaaga aacttctgta atggaagtgg agtgaagtga
     1741 ggaatcgtca aacggtaggg gataggctgt ttgaagggtg ctacgaaaaa gatagggtaa
     1801 ggtgaaatta tagcggaatg aaataagata tgaacaaatt gattagggaa atcaaatgaa
     1861 tttatagaaa tcttttagca gtcatggtgt cctattccag tttcgaaatg ctataaagac
     1921 gaacaaattg attgggaaat tcaaatcgct ttctatcaat gttttagaag atacagtgcc
     1981 ctagtccaac ttcaatatac tataaaagaa cagatggaag caattatgat ttacttctat
     2041 ctttaaaata aatgatctga taggaaaaat gtgtcccctc ataaaaaata ggatagtacg
     2101 gatggaaggc ttttgtagac tggaggacga aggaatttag aaactctcgt taaaagttag
     2161 ttgagccgga gtatggtggt gtgaataatt atcaaaaatt atttccaaat cgaaatagtt
     2221 gacagttaga acgatgagtg ttacaattat tttattcatt tcgacatcga aataaattgg
     2281 aggtgttatg aaagatagtc atttgctagc ccatcatatt cgtttgttga atgggcggat
     2341 ttttcaaaag ttactgagtc aagatcctga ggctctttat aggggtgaac agggcaagat
     2401 tttagcggtt ttatggaata gtgaaactgg ctgcgcaact gcgacagata tcgcgcttgc
     2461 gactgggctt gcgaataata cgctgacgac tatgataaaa aagctagagg aacaaaagct
     2521 tgtaattgtt agtccgtgtg gaaaagacaa gcgtaagaag tatttagttt taacggagtt
     2581 aggcaagtcc cagaaagaag tggggcatcg tgtcagtcag aaattggata ctatctttta
     2641 caaaggattt tcagaggaag aaattcacca atttgaaggt tttcaagaaa gaattttggc
     2701 gaatttgaaa gagaagggaa atgaggttta gaatatgtca caacaagtga aaaatgctca
     2761 caacctatac atccatgcca ttcaagacgg acgagttgct gaggctcaag cccagtctgt
     2821 aggggatacc tatatccaac attcgacagg tgttcctgac gggaaagaag gatttgcagc
     2881 cttctttgca gatttctttg agcgtcatcc agagcgtcag attaagattg tccgcaccat
     2941 tgaggatggc aatctggtct ttgttcatgt tcatcaatat ctgaatggtg gagaagctca
     3001 atgggtgacg acggatactt tccgtgcgga tgagaatggt cgtattgttg agcattggga
     3061 tgtcattgac tactatcgaa cacctgaaaa tgatcaacta gatcaaattt ttggagattt
     3121 tgaaatcaag gatttggata agaaagcaga aaataaaaag ctggttcgcc gtttcttgac
     3181 agaaattttc caaaatgggg agctagagca gtggagtgat tatgtggcag acgatttgat
     3241 tcagcataat catgagattg gacaaggaag tgctgcttat aaaaactatg tggctgaata
     3301 tagtgtcact tttgactttg ttttccaact cttaggacaa ggaaactatg tggttagcta
     3361 tggtcagact cagattgatg gcgttgctta tgcccagtac gatatcttcc gtttagagaa
     3421 cgggaaaatt gtggagcatt gggataataa ggaagtcatg cctaaggtag aagacttgac
     3481 taatcgaggg aagttttaaa ttgaggacaa agaatgattg aatacaaaaa tgtagcactg
     3541 cgctacacag aaaaggatgt cttgagagat gtcaacttac agattgagga tggggaattt
     3601 atggttttag tagggccttc tgggtcaggt aagacgacca tgctcaagat gattaaccgt
     3661 cttttggaac caactgatgg aaatatttat atggatggga agcgcatcaa agactatgat
     3721 gagcgtgaac ttcgtctttc tactggttat gttttacagg ctattgctct ttttccaaat
     3781 ctaacagttg cggaaaatat tgctctcatt cctgaaatga aggggtggag caaggaagaa
     3841 attacgaaga aaacagaaga gcttttggct aaggttggtt taccagtagc cgagtatggg
     3901 catcgcttac ctagtgaatt atctggtgga gaacagcaac gggtcggtat tgtccgagct
     3961 atgattggtc agcccaagat tttcctcatg gatgaaccct tttcggcctt ggatgctatt
     4021 tcgagaaaac agttgcaggt tctgacaaaa gaattgcata aagagtttgg gatgacaacg
     4081 atttttgtaa cccatgatac ggatgaagcc ttgaagttgg cggaccgtat tgctgtcttg
     4141 caggatggag aaattcgcca ggtagcgaat cccgagacaa ttttaaaagt gcctgcaaca
     4201 gactttgtag cagacttgtt tggaggtagt gttcatgact aatttaattg caacttttca
     4261 ggatcgtttt agtgattggt tgacagctct atctcaacat ttgcagttgt cacttttgac
     4321 cttgttacta gctattttgc ttgcgattcc cttggctgtt tttcttcgct atcatgagaa
     4381 gctggccgac tgggtcttgc agattgcagg tattttccag accatcccgt ctctggcctt
     4441 gttggggctc tttatccctt tgatgggaat tgggaccttg ccggctttga cagctctagt
     4501 gatttatgcg atttttccga ttttacaaaa tactattact gggctgaagg gaattgatcc
     4561 gagtcttcaa gaggctggga ttgcctttgg gatgaccaga tgggagcgtc tcaagaagtt
     4621 tgaaattcca cttgccatgc ctgttatcat gtctggaatt cggacggcag cggtcttgat
     4681 tatcggtacg gctaccttgg cgaccttgat tggggcaggg ggactgggtt cctttatcct
     4741 gttgggaatt gaccgtaata atgccagttt gattttgatt ggggcccttt cttctgcagt
     4801 gctagctatt gcctttaact tcctactaaa agtaatggaa aaagcgaaat tgcggacgat
     4861 tttctctggt tttgccttga tggctttatt actgggtctg tcttatagtc cagccctttt
     4921 ggctcaaaaa gagaaggaaa acttgattat tgctgggaaa ataggcccag aaccagaaat
     4981 tttggctaat atgtataaat tgctgattga agaaaatacc agcatgactg cgactgttaa
     5041 accgaatttt gggacgacaa gtttccttta tgaagctctg aaaaaaggtg atattgacat
     5101 ttatcctgaa tttaccggta cggtgactga aagtttgctt caaccatcac ccaaggtgag
     5161 tcatgagcca gagcaggttt atcaagtggc gcgtgatgga attgccaagc aggatcatct
     5221 agcctatctc aaacctatgt cttatcaaaa tacctatgct gtagctgttc cgaaaaagat
     5281 tgctcaagaa tatggcttga agaccatttc ggatttgaaa aaagtggaag ggcagctgaa
     5341 ggcaggcttt acgcttgagt ttaacgaccg tgaagatgga aataagggct tgcaatcaat
     5401 gtatggtctc aatctcaatg tggcgactat gcagccagct cttcgctatc aggcaattca
     5461 gtcaggggat attcaaatca cggatgccta ttcgactgat gcggaattgg agcgttatga
     5521 tttacaggtc ttggaagatg acaagcaact cttcccacct tatcaagggg ctccactcat
     5581 gaaagaagct cttctcaaga aacacccaga gttggaaaga gttcttaata cattggctgg
     5641 taagattaca gaaagccaga tgagccagct caactaccaa gtcggtgttg aaggcaagtc
     5701 agcaaagcaa gtagccaagg agtttctcca agaacaaggt ttgttgaaga aatgatggga
     5761 cagagacttt aaacatactg aactcaactt ccttaaacga ctagataaaa gaatgctcag
     5821 acaatttcgt ctgggctttt ttgatgtgag aatgactgaa tgtcacttaa aatggaaaga
     5881 aagagaaaat tttcatgatt ttctaaaatt ttactgtaaa gacagttgac tattcataaa
     5941 ataggtgtat aatattttgt gaactactta acaaataaga ggtttcagct catgtcgact
     6001 aaggttagaa atcttgtgta taggcagttc attatctaat agagcgttgc gtccgaaagt
     6061 ctatccagac acggctcttt aaaaacaaaa ggagaaatga tgcatactta tttgcaaaag
     6121 aaaattgaaa atatcaaaac aaccctaggt gaaatgtcag gtggttaccg tcgtatggtt
     6181 gcggctatgg ctgatttagg attttcagga actatgaagg ctatctggga tgacctcttt
     6241 gcccatcgta gttttgccca gtggatttat ttgctggttt taggaagttt tcctctctgg
     6301 ctggagttgg tttacgaaca tcgtattgtt gactggattg ggatgatttg tagcttgaca
     6361 gggattatct gtgtaatctt tgtatcggaa ggtcgagcaa gtaattatct ttttggcttg
     6421 attaactctg ttatttacct tattttggcc ctacagaaag gcttttatgg tgaggtgctg
     6481 acgacacttt acttcacagt catgcagcca attggacttc tagtttggat ttatcaggca
     6541 cagtttaaga aggaaaagca ggagtttgtc gcgcgtaaac tggacggcaa gggctggaca
     6601 aagtatcttt ccattagtgt gctttggtgg ttggcctttg gcttcattta tcagtctatt
     6661 ggtgccaatc gtccctatcg tgattcaatc acagatgcaa ccaatggggt agggcaaatc
     6721 ctcatgacag ctgtttaccg tgaacagtgg atattctggg cggctaccaa tgttttttca
     6781 atctatctct ggtggggaga aagcctgcaa attcaaggga aatatctaat ttatctcatt
     6841 aacagtctag ttggttggta tcaatggagc aaggcagcta agcagaatac tgatttactt
     6901 aactaggaaa agatgtttga aagtgctgtt ttgagatttt gattaaaaca gatatagttg
     6961 ataatcaagg atttatagta tgaaaaagag gatcggcggg tcctcttttg ttgttgaaaa
     7021 gataaaaaac tcagtaacct agaaataaga caactgaagc tttactctat attcaatttt
     7081 taggaatgag aaggtctaga taaaattgga caacttcctg gtctgtgaaa tcttgacctt
     7141 ttttgagcca ccaggtcaat gtctcgataa agttggacac gaccaagtgt tggaggtaag
     7201 aagtaggcat attagggtgg gcttctttta aattatcagc tagcacggaa tagacatggt
     7261 gttctagctc tttatggagt tgacggagga agtagtcatt tttggaaaat agcagactgg
     7321 tgatatggtc ttggttttta tgaaaatgga gaaagaggtg ggcgaggtag tcctcggttg
     7381 aaatggcttg ctctctttca aaaagatgat ggaagaggta gcggcagagc tggtccagaa
     7441 gaagctcctt actctcatag tgacagtaaa aggtggatct tcccacatct gcgagattaa
     7501 tgatatcctg aacagtagtg gcctcgtagc ccttagcatt caaaagttgt ataaaagctt
     7561 gatagatggc ttttttggtt ttgctgatac ggcggtcaat gttagtcata tggacactta
     7621 aggcaaattg ttcagaactg aataaagctg acgttttgct tctatccttt ctttgagttt
     7681 tagtggataa tgataatgaa caaggtgttc ataaacctat tataacaaag gaatgagaaa
     7741 tatgaaggca aaatatgctg tttgggtggc ttttttctta aatttgactt atgccattgt
     7801 tgagtttatt gcaggtggag tatttggttc tagcgctgtt cttgctgact ctgtgcatga
     7861 cttgggagat gtgattgcaa ttggaatatc agcttttcta gaaacaatct ccaatcgtga
     7921 agaagacaat cagtacacct tgggctataa gcggtttagc ctgctaggag ccttggtaac
     7981 agctgtgatt ctcgtaacag gctctgttct agtcattttg gaaaatgtca cgaagatttt
     8041 gcatccgcaa ccagtcaatg atgaggggat tctctggtta ggaattattg cgattactat
     8101 caatctgtta gcgagtctgg tggttggtaa gggaaagaca aagaatgagt ctattctgag
     8161 tctgcatttt ctggaagata cgctagggtg ggtagctgtt atcctgatgg cgattgttct
     8221 tcgatttacg gactggtata tcctagatcc tcttttgtcc cttgtcattt ctttctttat
     8281 tctttcaaaa gcccttccac gtttttggtc tacactcaag attttcttgg atgctgtgcc
     8341 agaaggtctt gatatcaagc aagtaaagag tggcctggag cgattggaca atgtggtcag
     8401 ccttaatcag cttaatctct ggactatgga tgctttggaa aaaaatgcca ttgtccatgt
     8461 ttgtctaaaa gaaatggaac atatggaaac ttgtaaagag tctattcgaa ttttcctaaa
     8521 agattgtggt tttcaaaata ttaccattga aattgatgct gacctagaaa ctcaccaaac
     8581 ccataagcga aaggtgtgtg acttggaacg gagttatgag caccaacatt agaaaaaagt
     8641 gaaaaatact tgggtactat cttatttgga atagagtaat ttctttatta tttaaatatt
     8701 tcaaaaattg gtaagagaag agcattgtat aaactccata tatatgattg ttaatgataa
     8761 aaatttttcg attagataca aaatgcttga cttggagtca actcaaagtt atataataag
     8821 ataagtgagt tagaatagcg tgaattcagt gaatgaaatg agaggaggtt agcgtgtgaa
     8881 tattaaatct gccagtgatt tgttgggaat ttcagcggat acgattcggt attatgaacg
     8941 ggttggtctt gtgccaccga ttactcgtac tgctactggg attcgtgatt ttcaagatca
     9001 ggatatcgaa gcgctggaat ttattaagtg ttttcgttcg gcgggtgtct ctgtagatag
     9061 tttagttgac tatatgtcgc tctaccaaaa gggagatgaa acgagagagg agaggcttgg
     9121 tattttagaa gaggaaaagc aaaaattaga ggagcgcttg tctcagctac agacagcttt
     9181 aaatcgttta aatctcaaaa ttaaacttta taaggaagga aaattttaaa tgaaatcagc
     9241 agtatataca aaggcaggtc aggttggact tgctagcatt gaacgtccgc aaataataga
     9301 agcggatgat gtgattattc gtgtggttcg tgcgtgcgtt tgtggttcag atttatggag
     9361 gtaccgtaat ccagaaacga aagctggaca caaaaatagt ggacacgaag cgattgggat
     9421 tgttgaagaa gctggggaag ccgttacgac ggtgaaacca ggtgattttg tgattgtccc
     9481 ttttacacat ggatgtggtg agtgtgatgc ctgtcttgct ggatttgacg gttcttgcga
     9541 caatcatatt ggcaataatt tggggggtga ttttcaggca gaatatattc gcttccacta
     9601 tgcaaactgg gcgctggtta aaatccctgg tcaaccttct gactatacag aagggatgct
     9661 ccagtccctt ttgactcttg cagatgtcat gccgacaggc tatcatgcgg cgcgtgttgc
     9721 aaatgttcaa aaaggggaca aggttgttgt tatcggtgat ggggctgttg gtcaatgtgc
     9781 tgtcatcgcg gctaagatgc gtggagcatc acaaattatc cttatgagcc gtcatgaaga
     9841 ccgtcaaaag atggctatgg agtcaggtgc gacagctgtt gttgcagaac gtggtcaaga
     9901 aggaattacc aaggtgcgtg aaatcctcgg tggaggagca gacgcagcac ttgaatgtgt
     9961 tggtacggag gctgctatag aacaggcgct aggtgttctt cataatggag ggcgtatggg
    10021 ctttgtagga gtcccacact ataataatcg tgctcttggt tcgacattta tgcaaaatat
    10081 ctctgtagca ggtggggcag cttctgctac aacatacgat aagcaatttt tactaaaagc
    10141 cgtccttgat ggtgatatca atccaggtcg cgtctttact tcaagttata aactggaaga
    10201 tatcgaccag gcctataaag atatggatga acgtaagacc attaagtcta tgattgtaat
    10261 cgaataaaaa acgaatagga gttttagaac tctattcgtt ttttatgtta tcctattctt
    10321 gatttagggt actttctctt aatgtcagtc tggttcccag catggtcagg ctagggattt
    10381 tccgaccgtg gaggacttcc ttgttaagaa tatccatacc tgctcgcccc atttcttcag
    10441 tatagactgt gatgctggag aggggaggat aaacctgctt ggtcagactg gtgtcgttga
    10501 atgaaatgag gctgacgcga tctggcaggc tgattccagc ttcttggagg gcacggaggg
    10561 caccgatagc taaactatcg ctggctgcga aaaatgctgg cggaagttgg tctcccaagc
    10621 tctgaatggc ctccttcatt aagtcatagc cagactgggc agtaaatctt ccttgaaaga
    10681 ccagttcatc atgatagatt cccctcgctt gactgtagtt tttgaagttt tctagacgct
    10741 tgtcctgaat gatttcttct tggtctgttg tttcctcaag ccctgttaga atcccgatgc
    10801 ggtccatccc ttgactgagg aaataatcga caacctgttt catagcagtg taaaaatccg
    10861 tgataataca ggtatgtccc agggaaagtg tatcgctgtc tagaaataca agaggctttt
    10921 ggtattcttc aaaggcagaa atctgagctc ggctaaattt tccgatgcag agaatcccaa
    10981 tcacttcctc gcttaaggta aaaggatggt cattaaaata gcgcaagata tcgtagtcca
    11041 actcttgggc tcttttttct attcctaggc gaatctggta gtagtagagg tcgtctagct
    11101 ccccttgttc gctgacccat tggataatgg caatcttttg cttgggcttg tgggactcgc
    11161 ctgtcttgag gtgtttggtg tagcctaact cttcagcaac ggttaaaata cggtgtctgg
    11221 tttcttctgt aacagatagg ctctggtcgc ggttgaggac acgggatacg gtcgcgattg
    11281 agacagaggc tagctgtgca atgtctttta aggtagccat aaatcctcct tgattaggtt
    11341 agtatatcat atttttcttc tttttactga tattttagta aaattttagt aaaaaggatt
    11401 gaccttggga aatttcttgg atacaataga aagaaaacga ttacacgtta agatggctta
    11461 acggacagtc aaaggagaat tcatatggca caacatctta ctactgaagt ccttcgcaaa
    11521 gactttcttg ctgtttttgg tcaagaagca gaccaaacct tcttttcacc aggccgtatt
    11581 aatttgattg gtgaacacac agactacaac ggtgggcacg tttttcctgc tgctatttcc
    11641 ttgggaactt acggtgcagc tcgtaagcgt gacgaccaag tcttgcgttt ctactcagct
    11701 aactttgagg acaagggcat tatcgaagtg cctctcgctg acctcaagtt tgaaaaagag
    11761 cacaactgga ccaattatcc aaaaggtgtc cttcatttct tgcaagaagc tgggcacgtg
    11821 attgacaaag gttttgattt ttatgtttat gggaatatcc caaatggtgc tggcttgtct
    11881 tcttcagcat ccttggagct cttgacagga gtcgtggcag agcatctctt tgatttaaaa
    11941 ttagagcgtc tcgatttggt taaaatcgga aaacaaacag aaaacaactt tatcggagtc
    12001 aactctggta tcatggacca gtttgctatt ggtatggggg ccgatcaacg tgctatttac
    12061 cttgatacca acaccttaga gtatgatttg gtgccacttg atttgaagga caatgtcgtt
    12121 gttatcatga acaccaacaa acgccgtgaa ttggcggact ctaaatacaa tgaacgtcgt
    12181 gctgagtgtg aaaaagcagt ggaagaattg caagttgcct tggatattca gaccctgggt
    12241 gaattggacg agtgggccgt tgaccaatat agctatttga ttaaagatga aaatcgtttg
    12301 aaacgtgctc gccatgctgt gcttgaaaac caacgtaccc ttaaagctca agcagccctt
    12361 caagcaggtg atttggaaac atttggtcgt ttgatgaatg cgtcacacgt ttctctggag
    12421 catgattatg aagtaactgg tttggaattg gatactcttg ttcacacagc ttgggcacaa
    12481 gaaggagttc tcggtgctcg tatgacaggg gctggttttg gtggctgtgc cattgccttg
    12541 gttcaaaaag atactgttga ggcctttaag gaagctgtag gcaaacacta cgaggaagta
    12601 gttggatacg ctccaagctt ctatatcgct gaagttgcag gtggcactcg cgtccttgac
    12661 tagtcaaaag gaggctctat agtgatctta gtagataaat ttgtaacaca tgtcatttct
    12721 gaaagttcat ttgaggaaat ggatcgaatc tacctgacca atcgtgtttt ggcacgagtg
    12781 ggagaaggtg ttttggaagt tgagaccaat ctggataaat tgattgacct caaggaccag
    12841 ctagttgagg aagccgttcg attagagacg attgaggata gtcagactgc gcgtgaaatc
    12901 cttggtactg aattgatgga cttggtgact ccttgtccga gtcaggtcaa tcgtgacttc
    12961 tgggaagcct atgcctactc tcctgagcaa gcgatagagg atttttatca actcagtcag
    13021 aaaaatgact acatcaaact caaggccatt gctaaaaata tcgcttatcg tgttccatct
    13081 gattatggag aacttgaaat taccatcaat ctctctaagc ctgaaaagga tccgaaagag
    13141 attgcggcag ccaagttggt gcaagctagt aattatcctc agtgtcaact ttgtttagag
    13201 aatgagggtt accatggtcg ggtcaaccac ccagctcgca ccaaccaccg gattatccgt
    13261 tttgaaatgg ttggtcagga atggggtttc cagtattcgc cctatgctta ctttaatgag
    13321 cattgtatct ttttagatgg ccagcatcgt cccatggcca ttagtcgtca gagttttgaa
    13381 cgtctgttgg ctatcgtaga gcagtttcca ggatattttg ctggatctaa tgccgacctg
    13441 ccgattgtgg ggggctctat tctaactcat gatcattatc agggaggccg tcacgtattt
    13501 cctatggaat tggctccctt gcaaaagacc ttccgatttg ctggttttga gcaggtcaag
    13561 gctggaatta tcaagtggcc aatgtctgtc ctacgcttga cttcggattc caaagaggat
    13621 ttgattaact tagctgataa gattttccag gaatggcgtc agtattcaga ttctagtgtg
    13681 cagattttag cagagacaga cgggacaccg catcacacca tcacaccaat agcgcgtaag
    13741 cgtgatggac agtttgagtt ggacttggtc ttgcgagaca atcagacttc agcagagcat
    13801 cctgatggca tctatcatcc ccacaaggat gtccaacata tcaagaagga aaatatcggc
    13861 ttgattgagg tcatgggctt ggcaatcttg ccaccacgtc tgaaagaaga agtggagcaa
    13921 gtcgctagct atcttgtagg agaagctgtt acagttgccg attatcatca ggagtgggca
    13981 gaccaactca aatcccaaca tccagaccta acggataaag aaaaagccct tgcaatcgtc
    14041 aaggactctg tgggggctat ctttgtgcgt gtacttgagg atgcaggagt ctacaagcag
    14101 acagagcagg gacaggcggc ctttatgcgc tttgtggaac aggtcggaat ttcactagac
    14161 taggagcttt ctccttgcac agtcctataa aaagtagtat catagtgtcg tttgaaatat
    14221 caggaggaaa cgatgaaaga ctttcatttt gacgctatat ctgcctttga aaattacgaa
    14281 attgaaaaaa tgagagatgg tcacgttgtg gtgacgacca aagtagtgaa ctcgtcgctc
    14341 aactactatg gcaatgccca tggtggctat ctattcacgc tatgtgacca aatcagtggt
    14401 ttggtggtta tctcgctggg acttgatggg gtgacactcc aatcctctat caactacctt
    14461 aaggcaggaa aactcgacga tgtattgacc attaaaggag aatgtgtcca tcaaggtcgt
    14521 acaacctgtg taatggatgt ggatatcacc aatcaagaag gcagaaatgt ctgcaaagca
    14581 acctttacca tgtttgtcac aggccggcgg tcagaagaca gacaggtaag gatataaaga
    14641 gtaatttaat agagtgaggt gaattttgga atttataaga tttgccttac tcattttttg
    14701 atattgatat gatttaattc taaaatagaa aatttagtaa tgtggtagaa aatacaagag
    14761 ttgtgttt
//
LOCUS       DAVLTM010000046        14201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLTM010000046 DAVLTM010000000
VERSION     DAVLTM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.047"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            37..1323
                     /locus_tag="VYN16_001177"
     CDS             37..1323
                     /locus_tag="VYN16_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1403712.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            1325..2194
                     /locus_tag="VYN16_001178"
     CDS             1325..2194
                     /locus_tag="VYN16_001178"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1403713.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            2270..2347
                     /locus_tag="VYN16_001179"
                     /pseudo
     CDS             2270..2347
                     /locus_tag="VYN16_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2489..3427
                     /gene="msrB"
                     /locus_tag="VYN16_001180"
     CDS             2489..3427
                     /gene="msrB"
                     /locus_tag="VYN16_001180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1403714.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            3485..5209
                     /locus_tag="VYN16_001181"
     CDS             3485..5209
                     /locus_tag="VYN16_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403715.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VLVAILTIVIVGLSRLVNPFYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            5211..6962
                     /locus_tag="VYN16_001182"
     CDS             5211..6962
                     /locus_tag="VYN16_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403716.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            7309..8568
                     /locus_tag="VYN16_001183"
     CDS             7309..8568
                     /locus_tag="VYN16_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1403717.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHIHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    8644..8779
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            8818..9318
                     /locus_tag="VYN16_001184"
     CDS             8818..9318
                     /locus_tag="VYN16_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1403718.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            9394..9762
                     /gene="rplL"
                     /locus_tag="VYN16_001185"
     CDS             9394..9762
                     /gene="rplL"
                     /locus_tag="VYN16_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1403719.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            9949..10119
                     /locus_tag="VYN16_001186"
     CDS             9949..10119
                     /locus_tag="VYN16_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403720.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            10119..10367
                     /locus_tag="VYN16_001187"
                     /pseudo
     CDS             10119..10367
                     /locus_tag="VYN16_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            10381..10721
                     /locus_tag="VYN16_001188"
                     /pseudo
     CDS             10381..10721
                     /locus_tag="VYN16_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10714..11094
                     /locus_tag="VYN16_001189"
     CDS             10714..11094
                     /locus_tag="VYN16_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1403721.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            11107..11250
                     /locus_tag="VYN16_001190"
     CDS             11107..11250
                     /locus_tag="VYN16_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403722.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            11343..11930
                     /locus_tag="VYN16_001191"
     CDS             11343..11930
                     /locus_tag="VYN16_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403723.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
     gene            12132..12269
                     /locus_tag="VYN16_001192"
     CDS             12132..12269
                     /locus_tag="VYN16_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1403724.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            12559..13962
                     /locus_tag="VYN16_001193"
     CDS             12559..13962
                     /locus_tag="VYN16_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV1403725.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT         4332 a         2547 c         3105 g         4217 t
ORIGIN      
        1 ataatggcta tattagaaaa tttcgaggag ataaagatga cagttaaaat tgctttacta
       61 ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa aggaaaatgg aggaaaaatc
      121 aatcaatcag cacattcaga tatcaaagtt gctaaggtat tggtcaagga tgaagatgaa
      181 aaaaatcgct tgcttgcagc agggaatgac tttaactttg taaccaatgt ggatgatatt
      241 ttatcagacc aggatattac tatcgtagtg gaattgatgg ggcgtattga gcctgctaaa
      301 acctttatca ctcgtgcctt ggaagctgga aaacacgttg ttactgctaa caaggacctt
      361 ttagctgtcc atggcgcaga attgctagaa atcgctcaag ctaacaaggt agcactttac
      421 tacgaagcag cagttgctgg tgggattcca attcttcgta ctttagcaaa ttccttggct
      481 tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa cttccaactt catggtgacc
      541 aagatggtgg aagaaggctg gtcttacgat gatgctcttg cggaagcaca acgtctagga
      601 tttgcagaaa gcgatccgac gaatgacgta gatgggattg atgcagccta caagatggtt
      661 attttgagtc aatttgcctt tggcatgaag attgcctttg atgatgtagc ccacaaggga
      721 atccgcaata tcacaccaga agacgtagct gtagctcaag agcttggtta cgtagtgaaa
      781 ttggttggtt ctattgagga aacttcttca ggtattgctg cagaagtgac tccaaccttc
      841 ctacctaaag cgcacccact tgctagtgtg aatggcgtaa tgaacgctgt ctttgtagaa
      901 tctattggta ttggtgagtc tatgtactac ggaccaggtg cgggtcaaaa accaactgca
      961 acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt tgaatgatgg tactattggc
     1021 aaagacttca acgaatatag ccgtgacttg gtcttggcaa atcctgaaga tgtcaaagca
     1081 aactactatt tctcaatctt ggctctagac tcaaaaggtc aggtcttgaa gttggctgaa
     1141 atcttcaatg ctcaagatat ttcctttaag caaatccttc aagatggcaa agagggtgac
     1201 aaggcgcgtg tcgttatcat cacacacaag attaataaag cccagcttga aaatgtctca
     1261 gctgaattga agaaggtttc agaattcgac ctcttgaata ccttcaaggt gctaggagaa
     1321 taagatgaag attattgtac ctgcaaccag tgccaatatc gggccaggtt ttgactcggt
     1381 cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc gaagaacgag atgagtggct
     1441 gattgaacac cagattggca aatggattcc acatgatgag cgtaatctct tgctcaaaat
     1501 cgctttgcaa attgtaccag acttgcaacc aagacgcttg aaaatgacca gtgatgtccc
     1561 tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt gctgggattg aactagccaa
     1621 ccaactgggt caactcaact tatcagacca tgaaaaattg cagttagcga ccaagattga
     1681 agggcatcct gacaatgtgg ctccagccat ttatggtaat ctcgttattg caagttctgt
     1741 tgaagggcaa gtctctgcta tcgtagcaga ctttccagag tgtgattttc tagcttacat
     1801 tccaaactat gaattacgta ctcgcgacag ccgtagtgtc ttgcctaaaa aattgtctta
     1861 taaggaagct gttgctgcaa gttctatcgc caatgtagcg gttgctgcct tgttggcagg
     1921 agacatggtg accgctgggc aagcaatcga gggagacctc ttccatgagc gctatcgtca
     1981 ggacttggta agagaatttg cgatgattaa gcaagtgacc aaagaaaatg gggcctatgc
     2041 aacctacctt tctggtgctg ggccgacagt tatggttctg gcttctcatg acaagatgcc
     2101 aacaattaag gcagaattgg aaaagcaacc tttcaaagga aaactgcatg acttgagagt
     2161 tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata gaagatagga tggggaaact
     2221 cttgaccaga gggtttcata tcctttttgt gaaaagaagt ttatactcaa tgaaaatcaa
     2281 agagcaaact aggaagctag ccgcaggctg ctcaaaacag tgttttgagg ttgtggatag
     2341 aactgacgaa gtcagctcaa gacactgttt tgaggttgca gatagaactg acgacgtcag
     2401 taacatatat acggtaaggt gacgctaacg tggtttgaag agattttcga agagtattag
     2461 ttaaaaacgt gataaaggag aaataaagat ggcagaaatt tatctagcag gtggttgttt
     2521 ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca gtgttggcta
     2581 cgctaatggt caagtcgaaa cgaccaatta ccagttgatc aaggaaacag accatgcaga
     2641 aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt tactttatta
     2701 tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg gccgccaata
     2761 tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca cagtggtgca
     2821 ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat tacgccacta
     2881 cattctggct gaagactacc accaagacta tctcaggaag aatccttcag gttactgtca
     2941 tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg aaaagcctag
     3001 tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac aagaagctgc
     3061 tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga tttatgtaga
     3121 tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag gttgtggttg
     3181 gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg atctgagcca
     3241 tggaatggag cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg gtcatgtttt
     3301 cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg cttctttacg
     3361 ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc cttacttaaa
     3421 caaataaaac agagagtggg gcttcccact ttcttcattt ctagaatatg aatagaaggg
     3481 atttatgaaa cacctattat cttacttcaa accctacatc aaggaatcaa ttttagcccc
     3541 cttgttcaag ctgttagaag ctgtttttga gctcttggtt cccatggtga ttgctgggat
     3601 tgttgaccaa tctttacctc agggagatca aggtcatctc tggatgcaga ttggcctgct
     3661 ccttatcttt gcagtaattg gcgttttagt ggccttgata gctcaatttt actcagcaaa
     3721 ggcagcagta ggttttgcta aggaattgac aaacgatctt tatcgtcata ttctttcctt
     3781 gccaaaggac agcagagacc gtctgacaac ttctagtttg gttactcgct tgacttcgga
     3841 tacctaccag attcagactg gtatcaatca attcctgcgt ctctttttac gagcgcccat
     3901 tatcgttttt ggtgccattt ttatggctta tcgaatctca gctgagttga ctttctggtt
     3961 cttagtcttg gttgccattt tgaccattgt cattgtaggg ttatctcgat tggtcaatcc
     4021 tttctacagt agtctcagaa agaaaacgga ccaactggtt caggaaacgc gccagcaatt
     4081 gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa cgagagttac agatttttca
     4141 aacccttaac caagtttatg ctagattaca agaaaagaca ggtttctggt ctagtttatt
     4201 aacacctctg acctatctga ttgtcaatgg aactcttctc gttattatct ggcaaggcta
     4261 tatttcaatt caaggaggag tgctcagtca aggtgctctc attgctctta tcaattacct
     4321 cttacagatt ttggtggaat tggtcaagct agccatgttg atcaattccc tcaaccagtc
     4381 ctatatctca gtcaagcgaa tcgaggaagt ctttgttgag gctccagagg atatccattc
     4441 agagttagaa caaaagcaag ctaccagaga taaggtttta caagtccaag aattgacttt
     4501 tacctatcct gatgcggccc agccttctct gagatacatt tcctttgata tgactcaagg
     4561 acaaattcta ggtatcatcg ggggaactgg ttctggtaaa tcaagcttgg tgcaactctt
     4621 acttggactt tatccagtag acaaggggaa cattgacctt tatcaaaatg gacgtagtcc
     4681 tcttaatttg gagcagtggc ggtcttggat tgcctatgta cctcaaaagg tcgaactctt
     4741 taaaggaacc attcgttcca acttgactct aggtttcaat caagaagtat ctgaccagga
     4801 actctggcag gccttggaga ttgcgcaagc taaggatttt gtcagtgaaa aggaaggact
     4861 cttggatgct ctagttgagg caggggggcg aaatttctca ggtggacaaa aacaaagatt
     4921 gtctatcgcc cgagcagtct tgcgccaagc tccgtttctc atcctagatg atgcaacctc
     4981 ggcactggat accattacag agtccaagct cttgaaagct attagagaaa attttccaaa
     5041 cacgagctta attttgatct ctcaacgaac ctcaacttta cagatggcgg accagattct
     5101 cctcttggaa aaaggtgagt tgctagctgt tggcaagcac gatgacttga tgaaatccag
     5161 ccaagtctat tgtgaaatca atgcatccca acatggaaag gaggactaga atgaaacgac
     5221 aaactgtaaa ccagacgctc aaacgtttag ccgtagattt agcaagccat cctttcctcc
     5281 ttttcctagc ctttctagga actattgctc aagttggctt atcaatttac ctacctattc
     5341 tgattgggca ggtcattgac caagtcctag tggctggttc atcaccagtt ttttggcaga
     5401 tttttctcca gatgctcttg gtggtaatag gaaatactct ggtacaatgg gccaatcctc
     5461 tcctctataa tcgtctaatc ttctcttata ccagagattt acgggagcga atcatccata
     5521 agctccatcg tttaccgatt gcctttgtag ataggcaagg tagtggagag atggttagtc
     5581 gtgtaaccac ggacatcgaa cagttggcag ctggcttgac catgattttt aaccaatttt
     5641 tcattggtgt tttgatgatt ttggtcagta ttctagccat gctccaaatt catctcctca
     5701 tgactctctt agtcttgctg ttgacgccac tgtccatggt gatttcacgc tttattgcca
     5761 agaaatccta tcatctcttc cagaagcaaa cagagacgag gggaattcag actcagttga
     5821 ttgaagaatc gcttagtcag cagactataa tccagtcctt caatgctcaa acagaattta
     5881 tccaaagatt gcgtgaggct catgacaact actcaggcta ttctcagtca gccatctttt
     5941 attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc actcatttat gcccttttag
     6001 ctggagtagg agcttatcgt atcatgatgg gttcagcctt gaccgtcggt cgtttagtga
     6061 cttttttgaa ctatgttcag caatacacca agccctttaa cgatatttct tcagtgctag
     6121 ctgagttgca aagtgctctg gcttgcgtag agcgtatcta tggagtctta gatagccctg
     6181 aagtggctga aacaggtaag gaagtcttga cgaccagtga ccaagttaag ggagctattt
     6241 cctttaaaca tgtctctttt ggctaccatc ctgaaaaaat tttgattaag gacttgtcta
     6301 tcgatattcc agctggtagt aaggtagcca tcgttggtcc gacaggtgct ggaaaatcaa
     6361 ctcttatcaa tctccttatg cgtttttatc ccattagctc gggagatatc ttgctggatg
     6421 ggcaatccat ttatgattat acacgagtat cattgagaca gcagtttggt atggtgcttc
     6481 aagaaacctg gctcacacaa gggaccattc atgataatat tgcctttggc aatcctgaag
     6541 ccagtcgaga gcaagtaatt gctgctgcca aagcagctaa tgcagacttt ttcatccaac
     6601 agttgccaca gggatacgat accaagttgg aaaatgctgg agaatctctc tctgtcggcc
     6661 aagctcagct cttgaccata gcccgagtct ttctggctat tccaaagatt cttatcttag
     6721 acgaggcaac ttcttccatt gatacacgga cagaagtgct ggtacaggat gcctttgcaa
     6781 aactcatgaa gggccgcaca agtttcatca ttgctcaccg tttgtcaacc attcaggatg
     6841 cggatttaat tcttgtctta gtagatggtg atattgttga atatggtaac catcaagaac
     6901 tcatggatag aaagggtaag tattaccaaa tgcaaaaagc tgcggctttt agttctgaat
     6961 aagccattct cttttgaaag tttatggacg aaaaaagttg ccttcgagtg acttttttgt
     7021 tacaatagct agaaaaattg ttcactgtaa tactcaatga aaatcaaaga gcaaactagg
     7081 aagctagccg taggttgctc aaagcacagc tttgaggttg tagataagac tgacgaagtc
     7141 agttcaaaac actgttttga ggttgcagat agaactgacg aagtcagctc aaaacactgt
     7201 tttgaggttg cagatagaac tgacgaagtc agtaaccaca cctacggcaa agtgaatctg
     7261 aagtggtttg aagagagtac aacttgtctt ttagaaaagg agcctataat gaaagtcttt
     7321 cagcatgtaa atatcgtgac ttgtgatcaa gatttccatg tttatcttga tggaatctta
     7381 gcagtcaagg attctcaaat cgtctatgtc ggtcaagata agccagcgtt tttagagcaa
     7441 gctgagcaga ttatagacta tcagggagct tggattatgc ctggtttggt caattgtcac
     7501 atccattctg caatgacagg tctgagaggg atccgagatg acagcaatct ccatgaatgg
     7561 ctcaatgact atatttggcc agcagaatct gagtttactc ccgacatgac taccaatgcg
     7621 gtcaaagaag ccctgacaga gatgctccag tcaggaacaa caacctttaa cgatatgtat
     7681 aatcccaatg gtgtggatat ccagcaaatt tatcaggtgg tgaaaacttc caagatgcgt
     7741 tgttattttt ctccgactct cttttcttca gagacagaaa caactgctga gactataagt
     7801 agaactcgat ccatcataga cgaaatctta aaatataaaa atccaaattt caaggttatg
     7861 gtagcacctc attctccgta tagctgcagt agagacttgc tggaagcgag tttggaaatg
     7921 gcaaaagagc taaatattcc gctccatgtc catgtggcgg agaccaagga ggagtcagga
     7981 attatcctca aacgatacgg caaacgcccc cttgcttttc ttgaagaact gggttattta
     8041 gatcatccgt ccgtatttgc tcacggggtc gaattaaacg agagagaaat tgaacgcttg
     8101 gcatcttctc aagtggctat cgcccacaat cctatcagta acctcaaact ggcatcagga
     8161 attgctccaa ttatccagct ccaaaaagcg ggagtagcag tcggaattgc gactgactcg
     8221 gttgcttcca ataacaatct agatatgttt gaggaaggaa ggactgcagc tcttcttcag
     8281 aagatgaaaa gtggggatgc cagccagttt ccaatcgaaa cagctctcaa ggtactgaca
     8341 atcgaagggg ctaaggtcct tggaatggaa aatcagatag gaagtctgga agtcggcaag
     8401 caagcagatt ttctggtcat tcaaccacaa gggaaaattc atctccaacc tcaggaaaat
     8461 atgctgtctc acctggttta tgcagttaaa tctagtgatg tagatgatgt ttatatcgcc
     8521 ggagaacagg ttgttaagca aggtcaagtc ctgacagtag aactttaaaa gaaaaattac
     8581 gaaaaatttt aaaaaaagtt tgcaaaaatc ttgcattctt tttttgacta tgctatactt
     8641 atatacggtt taaaaaaact gcctaagaca gtaggggagc tcgactcata aatatcctac
     8701 cgaggacaaa acgtatcatg taaaaagaag cgtattgtac tttcgtgtct aggtttgggc
     8761 gcgtttttct ttttgaaaaa ttccccaagc aaaataatta cggaggtgaa cacactaatg
     8821 agtgaagcaa ttattgctaa aaaagcggaa ctagttgacg tagtagctga aaaaatgaaa
     8881 gctgctgcat ctatcgtcgt tgtagacgct cgtggtttga cagttgagca agatacagtt
     8941 cttcgtcgtg agcttcgtgg aagcgaagtt gagtataaag ttattaaaaa ctcaatcttg
     9001 cgtcgtgcag ctgaaaaagc tggtcttgaa gatcttgcat ctgtatttgt tggaccatct
     9061 gcagtagcat tttctaatga agatgttatc gcaccagcga aaatcttgaa cgacttttct
     9121 aaaaacgctg aagcacttga aattaaaggt ggtgcaatcg aaggcgctgt cgcatctaaa
     9181 gaagagattc ttgcacttgc aactcttcca aaccgcgaag gacttctttc tatgctcctt
     9241 tctgtacttc aagcgccagt gcgcaacgtt gctcttgcag tcaaagcggt tgcagaaagc
     9301 aaagaagacg cggcttaatc ttaagctata cagcgtagcc tagctacgaa aaaaactatt
     9361 ataaaattta aaacttattt ggaggaaata acaatggcat tgaacattga aaacattatt
     9421 gctgaaatta aagaagcttc aatccttgaa ttgaacgacc ttgtaaaagc tatcgaagaa
     9481 gaatttggtg taactgcagc tgctcctgta gctgttgctg cagctgatgc agctgatgct
     9541 ggtgctgcta aagattcatt cgacgttgaa ttgacatctg caggcgacaa aaaagttggc
     9601 gttatcaaag ttgtacgtga aatcactggt cttggtctta aagaagctaa agaacttgtt
     9661 gacggtgcac cagcacttgt taaagaaggc gttgcaactg cagaagctga agaaatcaaa
     9721 gctaaattgg aagaagctgg agcttcagtt actcttaaat aatagagtat cgcaatttga
     9781 ttgcggaatc cctatttacc agtctttgag agcgataagc gattgataga aactgataaa
     9841 ttgatttggt ttcctgccaa aaattctttg gcaggatttt tgttttaata aactttcggg
     9901 ggtagacact agattgtcta cccctttttt tgtgtttcgg aggtaattat gcaaaaggaa
     9961 ggacagtcat cacgtcaatt cgtgaagagt catctcatga ctatcatggg agttgatatt
    10021 gtaaaagcaa ctcagttagt tgatgaaatg gaacaagtag gtttaatccg atttgatgaa
    10081 tttggaaatg ttggaatttt agtcttggag ggacaataat gaagcgtatt accgctaatc
    10141 aatatcagac atcagagcgt tattataagc tccccaaact tttatttgag agtgaacggt
    10201 ataagaatat gaaactggag gttaaggtgg tttactcagt tttaaaagat cggttggagt
    10261 tgtctttgag caaaggttgg attgatgagg atggggctat ttatttgatt tattccaatt
    10321 caaatttgat ggcactttta ggctgttcaa agtcaaaatt actctccatg tgagtttgaa
    10381 gtgacatttt tagatgatta ccataaaaaa cataactacc cactatttta cgaatcctat
    10441 cttcaaaacg ttatggaatt ccttgaaagt caagacataa agaatggggt tgatgccttt
    10501 gtagatgatc atcaaaatct cgtttttgtt ttatatggac aaggctatcg agccgaggga
    10561 aaagagggaa tacttacaac ccaagtaact gtaaaagctt atgatgaaga caagaaaccg
    10621 attaacttcg caaatttatt agattcctta atttattaga ttccttaatc gtgtcagaat
    10681 atcaaatgga accgaatctt tgggaggtct cccatgattg atctctatct aagtaaaaat
    10741 agacaaagaa atcaacttct tttagatttc ttccaaaact atggcataga ggtatcttgt
    10801 cattcagttt ctgaaatgac aaaggacaaa ttaattgaga tgatgagcta ttcttcagat
    10861 tgttttgagt ttttatctcc caatttatta cgatttaaga accgtgacaa tctaagatta
    10921 acggatttcg ttgagataat tttaaaaaat cctgaactaa ccatcagact tcctcttgcg
    10981 gtttcaaata agcgagttta tccaagtctg aatttggaag aggctagagc tctattgcca
    11041 agagatacga aacaattgat ttacatggcg caaacacatt atttatctaa ctaaaggaga
    11101 agcaatatgg ctgaacgatt ttgggagaac ttgtccatta ttttggctga aagaaatatc
    11161 agctggattg agttaaccag aaaaatgttt gcgggagagt ttcactatcc aagtgaattg
    11221 aatcgtttgt atcaaaagat tcgccactag aagatggaac aatgaatgcc tcaaagtcca
    11281 tgggtagaaa ggattgtgca gatattagat ttagattatg aagatttatt tcggaggtaa
    11341 ctatgaaaag aataattcca gtttatatat tccaacaagt aaatgtccta ttggtatctc
    11401 tatacttatt gaaatttctt tgtatcggtg agttaactat actacagatt ctctatggtt
    11461 cgtcactcat ttctttttta tggatgtatg gccaacgcaa acaagctcat aaggtaaata
    11521 tgaaatctag gatgaaatgg cttggtattg gattcgttag cctactgatt ataagtctat
    11581 gttttagcct aatccatgcg caagaaagca cgaatcaagc aaacttaatt ggacttcaac
    11641 atcaagttcc ttggttttca tttttattgt ttttaatcaa tgcgagtatg gttgaagaat
    11701 ttctgtatcg tgaaatttta tggaacttgg ttagaaaatt agatattcga gttgctttga
    11761 caagtgtttt atttgcctta gcacatcatc caggaaccat tatagcttgg tgtttgtatg
    11821 tatcgctggg gatgttttta gggcttgtac gctacaaatt ggacttatgg ggcagtatgg
    11881 ggctacattt ggtgtggagc ctactagttt atagtttgct gcttttttaa aataggtaac
    11941 ctgattttta ggtaacttct aaaactaact tccagttttg ggagagagat ggaagttact
    12001 ttgaaaagtt acggaaacat tattttctac gtgacaacat tgtatcattg cttgcatttt
    12061 actgctggag agagtataat gaaggtggat aaagcactta atcccaaaaa tttaaatagg
    12121 aggagttagc tatggcagaa gaagtattaa atcttcaatt agtatctgtt caagttgacg
    12181 aaacagatga agttgatgga atgcgattta gtacattcag tacaaaccgt tgcggtaact
    12241 ggagtgcttt tagttgggaa aattgttaat tcatttaaat tgtaaacttt agggattctc
    12301 tggagttatg gatctcgttc caatatgtgc acgttggaat gtcagtgctt atatgggggg
    12361 aatccaaagg attctccctt tttattttaa taagtctgtc tagctataga tatattaagt
    12421 ttgatagaat ttcttggaga ttttgttatg gattataatt tcaatttgga gcatcctttc
    12481 tttttcacta ataatgatta tagtacagat actagcataa agtactaagt aagtctgcct
    12541 tttaattggc acgaagtaat gaataatgat gagtgggttt atcaataccc tattggaaaa
    12601 tttgttgaaa gacaagggtg gaaaatacac attagttcag agtataatag ttcacacgaa
    12661 ttgttacaag atgtagcaaa aatttgccat gaaatgcgaa ttccatttaa acatcttagt
    12721 acagaagaca aatttatcat gcgaaacggg aaattggtta gtcgaggatt ttctggtaaa
    12781 tttattacat gttatccaaa tcaaaatgaa ttagagtcgg ttttgcaaag gttagaaagt
    12841 gccttaaagc aatataatgg gccatatatt ttaagcgata aacgttggga tgaagcacca
    12901 atatatctga ggtatggtgt gtttaggccg tcaagggatg atgaaaagaa ggttgttatt
    12961 gatgaattaa ttgttggcga tgaagttgtt aaagatgaac gtttaccagt atttaaaatt
    13021 ccaaaaggta tagtgccccc tgactttttg aataaatggc ttgacaagaa agataaaaaa
    13081 cagggagact ttccctttat tatagataat gcaattagat tttcaaacag tggtgggata
    13141 tataatgcta gactaaaaga agatgggaaa aaaataattt taaaagaggc tagaccgtat
    13201 acaggtctag gatttgatgg tacttattct tcagagagat tagcttcgga gtgcaaagca
    13261 ctaaaaattc taaatgaatg gagtgagatg cctaaaatat actggtacgg taagatctgg
    13321 gagcacacct tcttaggaat tgaacatatg aagggcgttc cactaaatag atgggttacc
    13381 aataattttc ctttatatga agttgttgat aagaccaaag attatctatt gagagtttca
    13441 aagatagtag aaaaactcat cgatttaacg aataagtttc attctgaaaa tgtatatcat
    13501 caagatttac atttaggaaa tatcttggtt aaggatgaag atgagattag tataatagac
    13561 tgggaacagg ctgtcttttc gaatgatgaa aaagttgtac ataaagttgc agcccctggt
    13621 tttagagcat ggcgagaaac acttcccagt gagatagatt ggtatggtat tagacaaata
    13681 gctcattatt tatacatgcc actagtaact acatcggatt tgacatacaa ctatgtaagt
    13741 caaaccagga tagaaggaaa aaaactattt gagtctttgg gttatacaag agaacatata
    13801 gattatgtag aaagtttatt gtcatatcta gattctaaat gtccacagat tgagaatatt
    13861 tccaggaaaa aagttttaaa acccatgcat gagattagaa ctatcgagag tgagcaagat
    13921 atacaagatt ttattataaa agcttttctt ttagtgaact gataggtatt ttcttagcaa
    13981 acgataaagt tttagggccg atacaatcaa ttgcctattc attaaataag ataaatacaa
    14041 ccaaagattt aaggaaaccg tttttaaaat acttaagtgg agagaagaat tttgtagacg
    14101 ctgaacatga taataacgga ctgtatactt catcaataga tgagatacac atgaaagatg
    14161 ttgtatattc tattacacca gaaaataaat taagtattga c
//
LOCUS       DAVLTM010000047        14034 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLTM010000047 DAVLTM010000000
VERSION     DAVLTM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.048"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(172..1779)
                     /locus_tag="VYN16_001194"
     CDS             complement(172..1779)
                     /locus_tag="VYN16_001194"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1403726.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2157..2744)
                     /locus_tag="VYN16_001195"
     CDS             complement(2157..2744)
                     /locus_tag="VYN16_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1403727.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2872..3321)
                     /locus_tag="VYN16_001196"
     CDS             complement(2872..3321)
                     /locus_tag="VYN16_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV1403728.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            complement(3326..3715)
                     /locus_tag="VYN16_001197"
     CDS             complement(3326..3715)
                     /locus_tag="VYN16_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1403729.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3743..4369)
                     /locus_tag="VYN16_001198"
     CDS             complement(3743..4369)
                     /locus_tag="VYN16_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1403730.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4383..4946)
                     /locus_tag="VYN16_001199"
     CDS             complement(4383..4946)
                     /locus_tag="VYN16_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1403731.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5111..5345)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5399..5902)
                     /locus_tag="VYN16_001200"
     CDS             complement(5399..5902)
                     /locus_tag="VYN16_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1403732.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6095..6925)
                     /locus_tag="VYN16_001201"
     CDS             complement(6095..6925)
                     /locus_tag="VYN16_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1403733.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6910..8592)
                     /locus_tag="VYN16_001202"
     CDS             complement(6910..8592)
                     /locus_tag="VYN16_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403734.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8703..8951)
                     /locus_tag="VYN16_001203"
     CDS             complement(8703..8951)
                     /locus_tag="VYN16_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403735.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVR"
     gene            complement(8958..9506)
                     /locus_tag="VYN16_001204"
     CDS             complement(8958..9506)
                     /locus_tag="VYN16_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1403736.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9521..10369)
                     /locus_tag="VYN16_001205"
     CDS             complement(9521..10369)
                     /locus_tag="VYN16_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1403737.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10558..11907
                     /gene="trkA"
                     /locus_tag="VYN16_001206"
     CDS             10558..11907
                     /gene="trkA"
                     /locus_tag="VYN16_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1403738.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11911..13350
                     /locus_tag="VYN16_001207"
     CDS             11911..13350
                     /locus_tag="VYN16_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1403739.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13476..13934)
                     /locus_tag="VYN16_001208"
     CDS             complement(13476..13934)
                     /locus_tag="VYN16_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
                     /protein_id="HEV1403740.1"
                     /translation="MEVVLFSYCLNTRYLLQIVDIASSNDIILEEDTSEIMPGEIDVI
                     KSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILA
                     PQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
BASE COUNT         4118 a         3211 c         2596 g         4109 t
ORIGIN      
        1 ttttatttca tttcaatata ctgataaatc cattcactag cacagggcaa gagggagtcg
       61 ctattgattt caacggtagc aggtacatac agaggactcc tacctcagat atactgcaat
      121 atcatcgtat gcaaaaaacc tctgagattt ttctcaaagg ttctgatttt gctaattgct
      181 gttctcaacc gctgcagtaa caaaggcagt gtagagttct tctggtcggt ttggacggct
      241 tgacagttca ggatgatact gacaagctac aaagaattta ttttcaggaa tttccacgat
      301 ttctaccaaa cgattgtctg gagaaactcc tgaaaagaca aaacctgctg cctcaaactg
      361 ctcacggaag gcattattaa actcataacg gtgacggtga cggcgttgca ccacttcttg
      421 attgtgataa gcagcagcag ccttagagcc acgtttcaac ttagacggat aaagtcccaa
      481 acgaagggtt ccacccatat cctcaatatc aatctgatca cgcatgatat caatgatagg
      541 gtattttgtt tctggtgcaa gctctgcaga attggcacct tcaagaccta aaacgtgacg
      601 agcaaactcg atacatgtca actgcattcc caagcagact cccaacattg gaacatcatt
      661 ttcacgcgca tagcggatgg cttggatttt cccttctgta ccacgttgac caaaaccacc
      721 tggtacgatg atcccgtccg catcagacaa gagttctgct acattctctg ctgtcacatc
      781 attggcattg acccaattga ttttaacttc tacatcattg acatagccag agtgtttcaa
      841 ggcttcgacc actgagatat aggcatcttg caactccaca tacttaccaa caagggaaat
      901 cttaacttgt ttcttgaggt tcatgacctt gtccaccatg gctgaccatt ctgtcatatc
      961 cgctgctggt gcgtctaatt tcaaatgatc acaaacaatt tggtccatcc cttgtgcctg
     1021 caagttcagt ggaatttggt aaaggtgttc aacatccaac gattcgataa cggcttctgg
     1081 tgccacatca cagaactggg ccagtttatt tttaattcct tgaccagctg gctcttctgt
     1141 acgaataacc aacatatttg gttggattcc caagccacgc aattctttga cagagtgttg
     1201 ggttggtttg gttttcattt caccagcagc cttgaggtaa ggaagcaagg ttgtatggat
     1261 atacatgaca ttatccgcac ccacatctgc cttcatctga cgaagagcct ctaggaatgg
     1321 caaggactcg atatctccta ctgttccacc aacctctgtg ataatgacat cagagtcggt
     1381 cgttagagcg gcacgcttga ttttttcttt caaagcatct gtgatatgag gaatgacttg
     1441 aacagttgcc ccaaggtatt ctccacggcg ttctttacga agaacttcac tgtaaatttt
     1501 cccagttgtc acgttggaat atttgttgag attgatatcg atgaaacgtt catagtgacc
     1561 caagtccaaa tctgtctcag ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg
     1621 actcatggtt cccggatcaa tattgatata agggtcaaac ttttgaatgg ttactttgag
     1681 accacgattt ttcaagagac ggcctagact cgctgccaca atccctttcc caatagacga
     1741 taccacacca ccagttacaa aaatatattt cgtagacata gattcctctt tctaaaatgc
     1801 tcaaggtctt gttaactacg aggggatata gaaaacaaga ccctactaat aagaataatc
     1861 tgggacgact gcaccagatt cacagctaat actcttcaaa ccgcgtcaac gtcgccttgc
     1921 cgtagatatg tgttcctgac tttgtcagtc ttatctacaa cctcaaaata gtgttttgag
     1981 cagcctacgg ctagtttcct agcttgctct ttgattttca ttgagtataa aatacaagaa
     2041 gataacttct tgaaaaaaca aaaatagctc cctaagaact agggagcccc gacctctaaa
     2101 agaggtgccc aaacaatatg atacctaaaa ataggataat tgtcaatagc taactcttat
     2161 tcctcgctgg tttcagcgtc gtcatctgaa aactcgtcgt cgtcttcgtt gagatccaca
     2221 tcttctccca agtcatctgg agcgatttcg ttgatttctg catcataagc ttccacttca
     2281 tttttttcat catctggatt ttcatcatcg tatgaaagag ctggatctgc ttcgtatgca
     2341 tcttcgtctt ctggatcatc tgcattgtag tcaatggcat ctgaatcacc atccataaag
     2401 gcattgacac gttttttctt agcttttggt gctacttcat cgtcgtcatt ttcttcaaga
     2461 gcgatgattt cttcgtcgat ttcgtccaca ccataccatg aacgaagacc ccatttgttg
     2521 tccccaagtg agatgaagct accgtcaaag ttcaactctg tgtagaacaa aggcaaagct
     2581 tcgcggatat cgctgtttga tgttccaagg tagttttgaa tttcgtttac aagatcgcta
     2641 aaatgcatct catgatcgcg accacgaagt tccaagatag cacgcgctac ctcaatcata
     2701 gatagttcac ttttttcttg cccagcaaat acttctaatt ccaaagcgtt tctcctcatt
     2761 tatactacta tcgccagagc gaacagactc tgacctcatt ttatcattta ctctttattt
     2821 tacgataatt ttgcagaata gtcaaaggtt aagggggaga aaatggcagg attagactaa
     2881 ttccaatata aaactcattc ctttttctgt tgctccattt tccacaaatc caagcgactt
     2941 gaaacacctc ctagaagcat gattgtaggt gtagatttcc ttgactctca attctttcca
     3001 tccttttact cgagccaatt caatcaaagc acttagaatc ttttttccaa gtcctcgatg
     3061 ttggtaagcg gaattcccaa tcacaatggg gagattatcc tgagatagtg taatatcccc
     3121 aattggaaac cattctccct tctccttgac ttcaatccaa aaaagctcac catgccgatc
     3181 caaataggaa tacatggctt ccaaggtcgc ttgactgtaa ggaagcttca ccccatctac
     3241 gaggtaaacc aagttcacat cctgatacca agcaaaagct tcctcacaat gattgacctt
     3301 ataataagga acaaaacaca atgcactacc ttttcaacaa aagagttgct gcttgattaa
     3361 aaccatcaca ccagttatac cattttgctt catacccatc ttgagctagg atacgatctt
     3421 ctaaatcaaa aacagagtaa atctttcttt cctcgcaagc ttgcgcatag agatgatata
     3481 gttcatcacc accatctcta tcccactcag cagaaatcgt atcccgatct gccaataaag
     3541 tctgataagc cctgtgatgc ccatctgtaa tcagcaaaca atctccaaag gcaagaatac
     3601 tgattggatc gacttggatt gtttctgccg actggtaaag catctgaata tcttgcaact
     3661 tcttttctga taaatatagt tgagtcggat gaagatctgc tatgttgact ttcatttctt
     3721 tctcctcaag ggaattcgat actcacttct gtttgccttt aaatcgccat tggaagcgga
     3781 gcttgtcata gaagggaaac tcgataaaca ggactcccaa gcccacacag agactggcaa
     3841 ggacgtctga tgggtaatga actcccagat agactcttga taccagcaca ctgactaggt
     3901 agaggccaag gacgatttgt acgatttttc tccagactgg atctttaatc cgctgactaa
     3961 gaataacaat caaagtacct accatcaagg ttacagccag agaatgccca cttgggaagg
     4021 aaaatccctt ctcctccacc agatgtaaga tatctggccg tgggcgctgg tagatatttt
     4081 taaaggtcac gattaaaaga cctgccaaag ccagatttcc cagcatgaag aaactttcta
     4141 tcttccatcg cttacgataa aagacaaaag ctgtaatgac aacccaagtg ataatcactg
     4201 ggatatcaat cagacgtgtg agggctcgaa aaagaatagt caaataatct ggtaagtctc
     4261 ctcgaatggc agtctgaatc gattggtcaa aattgaccag cgtttcaggg taaaatttga
     4321 ccatgtagcc aagaataacg aaaagtaaaa gggcaaaact gcccttcatt aaaaatgttt
     4381 gtttatctct cataatgttt taaggttggt ttcaagagaa catacaacaa ccagaatgaa
     4441 acggaaaaga taacaccttc aatcaagtta aaaggtaata ccatggtcat taggtagttg
     4501 gaaagtccca aaatttttcc aatatcaaag ttagcaaact tagcgtacaa aggaacagca
     4561 taaacatagt tgagaaccaa catggccaag gttaaaccaa tagttccagc tagagagcct
     4621 agtaggaaac gaagggttgt ccgttccttt ttccaaatca aagcaaatac gatgacaaaa
     4681 actcccaaag ctacgatatt catcggcaaa ccaatgtaag tattcactcc ttggctgtta
     4741 agaagcaact tcaagagtga gcgaagcaag agcactccta gagcagcagg caaatccatg
     4801 accaccagac ccacaaggac tggcaagata ctaaattcga tcttgaggaa agatgccgct
     4861 ggtaaaagcg gaaaatcaaa gtacatcagc acaaatgaga tggctgatag aattgcaatg
     4921 gtcgaaagtc gacgtgtgtt tgtcataaca ggttcctcca attttctata aaatcagaag
     4981 aagttggaaa ggattcctct atctattctc actttttata tcccaaaagt tccctcttac
     5041 tctattaaag aaaaacaaag caagtggtta caatccggct ataaatctat caaaacagac
     5101 aaagctattc tttcgtcttc tcccatccag actatactgt cggttgtgga atctcaccac
     5161 atcagcttgc gctcgcggac ttctttaaag agaaagaaat agagattttt cttttagact
     5221 aggcgacaag ctgtaggcat aacttctggt taggcaaagc ttgtcaacaa agtataaaag
     5281 gaaaagatca tttcctagtt aaagtcaccg ccggtcggga atcacaccct gccctgaaga
     5341 ctcttttatc ataacaaaaa acgcttgcaa gcgcaagcat tttgttcatt ttcatttttt
     5401 atttcaattt atccacttca taggtatgga cgagctcaag acctgcaaag ttctgctggc
     5461 gaagagcctc atagacaatc atgcagacgg tattagacac attgagactg cggacatgtt
     5521 catcattcat gggaatacgg agagccttct caggatgttc tcgcataaaa tcttcaggta
     5581 agcccttgtc ttcacgtcca aagagaaaat aatggtcctc gtcagtcgat aaatccacct
     5641 cagaatagat tttttccgcg aatttcgaaa tcagatagag ttttcccttc atctgagata
     5701 tgaaatcttc taaactctcg taaaaataaa tctctagctt atcccagtaa tccaatccag
     5761 cccgcttcat cttgcggtca tcaataggaa agcccatagg cttgatgatg tggaggggag
     5821 aattggtcgc agcgcaagta cgcgcgatat tgcctgtatt ttgtggaatt tgaggttcaa
     5881 ataatacaat gtgatttgtc atgacttgct tcctttcacc attgcaaaaa aatagccaca
     5941 ctgcccggag tcaagctcag caaacagcgt ggttaaggca tcgttaactt acctcacaac
     6001 aggtttgaag taaatcagcg aaactacttt cttagtataa cactttcaga atcattgtca
     6061 atagaaatga cttgattttt tcaatttttt caagctattt ccaagggttg taaaatcgtc
     6121 cctgattctg caagataagt agtaaactaa ctactaaaaa caaggttgcc aagagcaagg
     6181 taatatagtc tccttttttc aaggcctgat aactatacca tgtgcgtttt ttctctttcc
     6241 caaagcggcg aagctccatg gcagtcgcaa tggtatcaat gcgttctagc gagctaaaaa
     6301 tcaagggcgt aataatgagc agattgcctt tgattcgttg cataagagaa gctttcttgg
     6361 ataattccat cccacgcgcc tcctgagaca tcttgatagt aaagaattct tcctgcaaat
     6421 ctggaatata gcgcaaggtc aggctgacag aataagcaat cttatagggc acaccaattt
     6481 gatttaaact ggaagcaaac tgactaggat gggttgtcat caaaaagata atagccagag
     6541 gaatggtgca aagatactta atggccaaat ttagcagata aaagagctcc tggctggtta
     6601 gagtgtagac accgattccc tgccaaatca cacttctctc tccataaagt ccaaccccat
     6661 actcgggaga aaagagatag accatcaaaa cgtttaaaac ggcaaatacc gtcgcaaaaa
     6721 cggctacaaa ggaaacatct ttaaagcgaa tttctgataa atagaggaga aagactgaaa
     6781 agatggcaat cagcaagagc attctggtat catagctaat catggccgcc aatgatacca
     6841 gaatgaaaaa gagaagtttc ccagctcctg acaagcgatg aatcacagta tctctatgct
     6901 ggtaaccgat taatttagct tgcatccctc tctcctttct ttgtaaaatg ccgttaaatc
     6961 cagtggatcc acatctagtt tcttagccaa gttaaagatg gaggtttctt ttagattggc
     7021 ttttactaac agctcaggat cgctcaacag actggctgga acagtatcgg caatcaattc
     7081 tccatccacc atgacaagga cccggtctga ataatccagc atcaattgca tatcatgggt
     7141 aatcatgaca atggtatgcc ctttttgatg taactcttcg agaaattcca taatctcagt
     7201 atagttcttc tgatcttgac ctgcagtcgg ttcatctagg agaataattt cagctcctaa
     7261 gaccaaaatt gaagcaatgg tgacacgttt tttctgacca aatgacaggg cagaaatagg
     7321 ccaattacgg aattcataaa gtccacagat tttcaaggtt tcatagactc tcgtttcaat
     7381 ttccttctca tccacacctc gcaaacggag ccctagagcc acctcatcaa aaatcatatt
     7441 ggttgaaatc atttgattag gattttgtag cacatagcct actcgttccg cccgctctgc
     7501 aacagaatcg ccttttatat cctgtttttc ccaaagatag cgtccttccg tctgaataaa
     7561 gctacttata gccttggcta gagttgattt ccctgctcca ttttttccga caatagcaat
     7621 cttttcaccc tttttaatat ctaaatgtag ggattttaaa atcggtctat catcataaga
     7681 gaaagacacg tcctctaatt taaagagtga ctgcaattct ggagtttctt ttgccagttc
     7741 attctgcagc tgaacctgac cttttgagat agacatgtta tccagattcg ctaactgttc
     7801 ttccttgact aagtccacac ctaattgacg gagagtcgtt agataaaggg gttctcgaat
     7861 tccattttga gtcaataaat cagtcgcaag caactggtca gggctcccat taaaaaggat
     7921 acgaccatcg tttatcaaga caatccgatc cacagggcga tgcagaacat cctccaaacg
     7981 gtgctcgata atcagagtcg tcgtcccctc ttccttatga atctggtcaa tcaattcgat
     8041 aatatcctga cctgacttgg gatctagatt ggcgagtggc tcatcaaaca agagaatcgg
     8101 actttcatca atcaagacac cagccagact gactcgctgc ttttgtccac ctgacaaatc
     8161 ctgaggacgc tgatccagta aaggaagaag gtccagcttt tcagcccatt tataaacacg
     8221 acctttcatc tcatctaggg ctgtcacatc attttccaga gcaaacgcca aatcttctgc
     8281 cacagacaag ccaataaact gcccatctgt atcctgcaaa actgtgctaa ccagatgaga
     8341 cttatcatag atgctcatat caaaggctac ttgacccttt atcaaaaatt ctccatatgt
     8401 ctgacccttg taaatattgg gaataatccc attcaaacac tgacccaagg tagatttacc
     8461 tgacccagat ggtccaacaa ttaagacttt ctctcccttg taaatggtca agtctatccc
     8521 ttgcaaggtc ggttcttgtt gtgtttcata ccggaaagag aaatccttcc actcaattat
     8581 agcttctttc atcttactct cttcattcgc ttcttagact tctattttat cataaatcaa
     8641 gcccttcttg cagtctctcc tcttaaaatc ttagcgccaa aaagattcct atcctagctt
     8701 acttacctaa ctaatctata aacatcgaaa aagactagtt gcccagcctt ccccatcatt
     8761 ttatactctt cgaaaatctc ttcaaaccac gtcagcttcg ccttgccgta ggtatggtta
     8821 ctgacttcgt cagtttcatc tacaacctca aaaccatgtt ttgagctgac ttcgtcagtt
     8881 ctatccacaa tctcaaaaca ctgttttgag caacctgcgg ctagcttcct agtttgctct
     8941 ttgattttca ttgagtatta gtcctttttc aaacttcctg cacgagtttg ggttcctgca
     9001 taggcaagta agagaagagt tcctgcaata gctacagata caccattggc aattcccgca
     9061 acaatccctt gtgcaaatac tttttctgcc gcttcttgat aaatcacaac atctccaagt
     9121 ggtgccaaga caccccaaac aagggcattt gcaagtagtt gaatgaggtt aaaaataaga
     9181 atatctttcc agtcaaaaac accattgatc actcgaacgt actttctaaa aagtcccaca
     9241 actagaccaa agagtccgct agcgataatc caagtccacc atagaccata accagcaaga
     9301 gagtccttga ttgcatgacc aatcaacccg acaagcaaac cgataatcgg tccaaaaata
     9361 atagaaagta gcgcttgtac cgcatactga agctggatgc ttgtatttgg aacaggggtt
     9421 ggaatgttga tcatcccgat gacgacaaag agggcagcgc caattccgac agcaacaact
     9481 tgtttaattg taaatttgat ttccatacta ttctcctatt ttatccttct attttcttta
     9541 tttcaatggt ccaagatgaa ccgacaccta cattataggc cttggcaaag gaaccttggt
     9601 tgatagccaa acctaaacga tagagagagt tgatgtaaag gatgggttgc ccaattctca
     9661 catctgcaaa tgatttgcca tagacaacct gattttgata gaccagcata tcagcatgat
     9721 agatggtcac ttcaaaacga tcaccaaatt ctggttccag cttgtaaaat tcttcccgtg
     9781 tgatagaggt ccaaagcgaa ccgaaacgca catccagaat atcaatggct cccttcacca
     9841 gatgatcttc tatgatggtc gctacgactg gaagctctac aatctgttcc acactgagct
     9901 ctggccctac ttcctcaaaa gtaatgtgac cactggccag tttagcacca gtataggcat
     9961 agacatcacg accgtggaag gtataagaat gctctgtgtt ttgacgccta ttggccacct
    10021 cagaaatctc acgaatggct acaatgccaa cgtgtttctt gataaaggaa agcgtcccat
    10081 tatctggcgt gacaatgtat tgattttttg cagtcttggc aactacactc ttacgtttcg
    10141 aaccgacacc tggatcgaca accgatacaa acgtcgttcc ctcaggccag taatccaccg
    10201 tctgaaagag acgatagctc ccctcaaaaa tattataagg cgtgatatcg tgcgtcaagt
    10261 gatggatttt taaggttgga gactcttcta aagccactcc aatcatagcc gataccgcac
    10321 catcaaccag accaaagtct gattgtaata ccagtaaatt attattcatt ttatctcctt
    10381 gtatatcctt tatcatacca tatttcagag aaagtcgcta atagctattt caattggtta
    10441 gggtatagtt ttaccttata gcaaatttcc tactaaaagt tcttgttatt agctagtagg
    10501 tgcatttttt cttgaaaaag ggtatgatag ttacatcatg aaaaagtagg ttttattatg
    10561 aaaattatcc ttgtcggagg gggaaaagtt ggttttgccc tctgtcgctc cttggttgca
    10621 gaaaagcatg atgttttgct gattgagcaa gacgaagctg ttctcaatca tattgtcagt
    10681 cgctttgata tcattggtat ccttggtaat ggggccgatt ttgccattct tgagcaagcc
    10741 agcgtccaag attgtgatat ctttatcgcc ctgactgagc acgatgaagt caacatgatt
    10801 gcagcagttc tagccaagaa aatgggagct aaagaaacta tcgttcgggt gcggaaccct
    10861 gaatattcta actcttattt caaggaaaag aatattctcg gtttttctct tatcgttaat
    10921 cctgagctct tggctgcccg cgctatcgcg aatatcattg acttccccaa cgccctgtct
    10981 gtcgaacgct ttgctggtgg acgcgttagc ctcatggaat ttgtcgtcaa gtccaccagc
    11041 ggtctttgcc aaatgcccat ttctgatttt cgtaaaaaat ttggtaatgt cattgtctgt
    11101 gcgatagaga gggatcatca aattatcatt ccaagcggtg acatgactgt acaggataaa
    11161 gatagaatct ttgtcactgg taaccgtgtc gatatgatac tcttccataa ttattttaaa
    11221 tcacgcgccg tgaagagcct tctcatcgtt ggggcaggta gaattgccta ttatctactt
    11281 ggtattctca aagatagtcg tatcgataca aaagtcattg aaatcaatcc tgaaatcgcc
    11341 agcttcttta gcgagaaatt cccaaatctc tacatcgttc aaggagatgg aaccgcaaaa
    11401 gatatcctgc tggaagaaag tgctcaacac tatgatgccg ttgcgactct aacaggtgtc
    11461 gatgaggaaa atctgattac atctatgttc cttgacaggg taggtgtaca gaaaaatatt
    11521 actaaggtca atcgtaccag tctcctcgag attatcaatg cgcctgattt ttcaagtatc
    11581 atcacaccta aaagcatcgc tgtagatacg attatgcact ttattcgtgg tcgagttaat
    11641 gcccagtatt cagaccttca agccatgcac catctagcca atggccaaat tgaaaccctg
    11701 caattccata tcaaggaagc caataaaatg actgccaaac ctctttctca actgaaattg
    11761 aaaaaagggg ttcttattgc agccattatt cgaaagggca agactatttt cccaactggg
    11821 gaggatatgt tggaagttgg agacaagctc ctagtaacaa ccttgttgcc aaacatcacc
    11881 aagatttatg acttgattgc gaggtaagaa atgaataaaa gtatgattcg ttacctcctt
    11941 tcaaagttac ttttgattga agctgttctc ctcttggttc ctgtggctat cgctgtctat
    12001 taccgtgaat cgagccaagt ctttacagcc ctcttttcga ccatagggat tctcgtattg
    12061 ttaggcggtt cagggagttt acagaagcca aaaaatcaac ggatttatgc caaggaggga
    12121 gtcttgatcg ttgccctctg ttggatcctt tggtctttct ttggtggtct cccctttgtc
    12181 ttttctgggc aaatccctag cgttattgat gccttttttg aaattagttc tgggtttaca
    12241 actactggag caagtatttt gaacgacatt tcggttctca gccgttccct cctcttctgg
    12301 cgaagtttta cccacttgat tggagggatg ggagtgcttg tttttgcact tgctattatg
    12361 gacaatgcca aaaatagcca cctagaggtg atgaaggctg aggttccagg tcctgttttt
    12421 ggcaaggttg tatccaaact aaaaaacact gcccagattc tctatctcct ttatctagct
    12481 ctcttctccc tctttgtcat catctattat ctagctggta tgcctctcta cgatagtttt
    12541 gtcattgcta tggggacagc gggaactgga ggctttaccg tctataacga cggaattgcc
    12601 cactatggca gctcactgat tacctatctg gtcagtatcg gagttctggt ttttggggta
    12661 aatttcaacc tctactacta cctcatgctc cgtcgcatca aggccttctt tggtgacgaa
    12721 gaacttcggg cttacttggt cattgtactg gtttctacag gcttgattag cctcaacacc
    12781 ctctacctct acccagggtt ttcaaagagc tttgaaatga ccttcttcca ggtttccaac
    12841 atcattacaa caactggttt tggatatgga gatattacca actggcccct cttctcccag
    12901 tttatcctcc ttttcctcat ggcaatcggt ggctctgctg gatcaactgc aggtggactc
    12961 aagattattc gaggcctcat cctttcaaaa attgccaaaa accaaatttt gtcaatccta
    13021 tcgccccacc gtgttttgac cctccatgtg aatcaaacgg tgattgataa agatacccag
    13081 cataagattc tcaagtactt tgtcatctat gctatgattt tgctagcgct tatctttatt
    13141 gtcagcctag atagcaatga ttttctggtc gtgaccagcg ctgtctttag ctgtttcaat
    13201 aatatcgggc ctattctagg aaccacttct agtttctcaa tctttagtcc catctcaaaa
    13261 attctcctat cctttgcaat gattgcaggg cgcttggaga tttacccaat cctccttctc
    13321 tttatgaaga gaacttggtc taagagataa aatacaaaat ccctcttttt acaattgtga
    13381 aagagggatt cttatattaa aaaaggggga acgtttttaa atccaagcct atctgtaagc
    13441 aaaatcctta ctcaaataaa agatggaaaa acttactaca cagcttggta atgctccaat
    13501 acaaattgta aggatttact tggactcttt gttaaatgaa tatattccat tcctctggta
    13561 gcaactatct gaatacctaa tctttctgca tccaccttca tctctctata ataactccga
    13621 acttgtggag ccagaataat taaatcataa acacccataa tatcataatg agctccgtac
    13681 gctcctgaat tcgcaatcac tctaacttct gctaattgag cgccctcgtt aattgcattg
    13741 gctaattgcg cacttgttcc agaccctgca caaagaacca gtactttcaa ctccttactt
    13801 tttattacat ctatctcacc aggcattatt tcacttgtat cctcctctaa aataatatca
    13861 tttgagcttg caatatctac aatctgtagt aaatatctcg tatttagaca atatgaaaac
    13921 aagacgactt ccatatagga aaccgccttc tcgctatgtt gagtgattta tattaaaata
    13981 acttttcttc tagctgcatt ttattattat aaaaacattc atcataaccc ccag
//
LOCUS       DAVLTM010000048        13728 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLTM010000048 DAVLTM010000000
VERSION     DAVLTM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.049"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(142..543)
                     /locus_tag="VYN16_001209"
     CDS             complement(142..543)
                     /locus_tag="VYN16_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1403741.1"
                     /translation="MLFFSISNDLLQLLTSWKEQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISKALT
                     HSDTGTTQIYVNTSNVVPMTVGEFALKSLKQ"
     gene            complement(919..2127)
                     /locus_tag="VYN16_001210"
     CDS             complement(919..2127)
                     /locus_tag="VYN16_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1403742.1"
                     /translation="MNYIKRPHYLDFLRRHRDRQIIKVVSGVRRAGKSVLFQLYKEEL
                     IATGVDEDQIISINFEDLSYYDLRHFQTLFAYIKEQLIGEKTYYIFLDEIQHVEKFEL
                     VADSLFILPNVDLYLTGSNAYFMSSQLATNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYAEKIDYLRGIYNSILLNDIVTRLGNPNPTIIERIV
                     RTLLSSTGSLISTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDHKEDIGHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLDAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KNIIDWLLEK"
     gene            2532..2708
                     /locus_tag="VYN16_001211"
     CDS             2532..2708
                     /locus_tag="VYN16_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403743.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            3109..5322
                     /locus_tag="VYN16_001212"
     CDS             3109..5322
                     /locus_tag="VYN16_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1403744.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(5544..6020)
                     /locus_tag="VYN16_001213"
     CDS             complement(5544..6020)
                     /locus_tag="VYN16_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1403745.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            6243..9446
                     /locus_tag="VYN16_001214"
     CDS             6243..9446
                     /locus_tag="VYN16_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1403746.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIETKDG
                     TITISSPEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WLHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKGINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9799..10184
                     /gene="tnpA"
                     /locus_tag="VYN16_001215"
                     /pseudo
     CDS             9799..10184
                     /gene="tnpA"
                     /locus_tag="VYN16_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10563..11192)
                     /locus_tag="VYN16_001216"
     CDS             complement(10563..11192)
                     /locus_tag="VYN16_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1403747.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(11202..12203)
                     /locus_tag="VYN16_001217"
     CDS             complement(11202..12203)
                     /locus_tag="VYN16_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1403748.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISALEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKETYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(12234..12875)
                     /locus_tag="VYN16_001218"
     CDS             complement(12234..12875)
                     /locus_tag="VYN16_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1403749.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12894..13709)
                     /locus_tag="VYN16_001219"
     CDS             complement(12894..13709)
                     /locus_tag="VYN16_001219"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1403750.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4356 a         2328 c         2837 g         4207 t
ORIGIN      
        1 gtttcaaagc actcaaaagt tcaccttatt tttatagtaa aatgaaataa gaataggaca
       61 aatcgatcag gacagtcaaa tcgaattcta acaatgtttt agaagtagag gtgtactatt
      121 ctagtttcaa tctactatac cttattgttt tagagacttt aaagcaaatt cacctactgt
      181 cataggaact acattagaag tatttacata aatctgtgtt gttcctgtgt cactgtgtgt
      241 tagagcctta gaaatggctt ctaagctcat tcctgctttt ttagcaagtg tcgctcctgt
      301 atggcgtaat ttgtgaggtg tagcgtgtgt cagttcttta tgtcgctttc taatgctttt
      361 cattttattg ttcagatagt cggcatgtaa aggcttgttg atgtttcctt ttgtgtcgat
      421 atatgtaaaa acaaattgtt ctggattact gaggatgcca aactttgcta gttcatatct
      481 ttgttgttct ttccaagagg taagaagttg aagtaaatca ttagaaatag agaaaaatag
      541 gatagcaaaa gctctatttc aggagaaaat gaagcaaata tttccataat aaaacgcata
      601 ttatcaaggt tgttcaatac ctgatactat gcgcttttct gattttaaag actgattgct
      661 cagtctcttt ttcattataa ttcagttaca atttaatctt atttgagaaa gtgggtgatg
      721 ctattctcta ataagactat gatattaatt tttgatagta cttccagaaa ttatagatcc
      781 cctttcatcc ccaatttcta ttttcaagtg tccgtattat atagcgtatt cgaaattcac
      841 tatatcttgt taggatttgg gtagaatcat tgataacaga ctgttgcaaa tctaattagc
      901 tgtatgattt atatttatct atttttctag taaccaatct ataatgtttt tcttctcgat
      961 tccattgtaa ttggctgttg gctgaatcgt atccattgtt aatagatact ttgggaattg
     1021 atctttaatg gcgtctagtg gtctaagttc tctttctagt gtttctggag caagtgttgt
     1081 ttcactgact tgataataag cgtagtgacc aagatcagtt acaacaacaa aatcaacctc
     1141 atatttatct aaattaccaa catatacttg tgaatatcta cgtctcaatt ccaaatagac
     1201 catattttcc aagatatggc caatatcttc tttgtggtct ggcaataaga ggtgtcgtaa
     1261 acctaaatca acgggataat atttttctaa gcgttgcaat aatgctctac cttttacatc
     1321 aaaacgtgga acggaataaa aaagcaaact atctgtcaaa gtcgtcaaat aattttccaa
     1381 agtattatgg gagattgaaa cgttttggct gaccagggta ttgcgaatct tatttgttga
     1441 tattaagcta cctgtactac tgagaagggt tcggacaatg cgctcaataa tagtaggatt
     1501 tggatttccc aatctagtga caatatcatt taacagtatg gagttatata ttcctcttag
     1561 ataatcaatt ttttcagcgt aagatgatgt ttgcaataag taagggaaag cactaaagag
     1621 ataattgtta aaaatttctg ttgtattcag attttctgtg agagattgac ctgatagata
     1681 ttcttcaaat gacaatggaa gaacttctat ctcaacatac cgaccggtca agtttgtagc
     1741 taattggcta ctcataaagt aggcgtttga tccagtcaaa tagaggtcaa catttggtag
     1801 tataaataga ctatctgcta ccagttcaaa tttttcaaca tgttgaattt catctaaaaa
     1861 gatatagtat gttttctctc caattaactg ctcttttata taagcaaata atgtttgaaa
     1921 atgtcggaga tcatagtaac tcagatcctc aaaattgatg gatatgattt gatcctcgtc
     1981 taccccagtt gctattaact cctctttata gagttgaaag agaacagatt taccagctcg
     2041 cctaactcca ctcacaactt tgatgatttg tcggtcacga tgtcttctta aaaaatctaa
     2101 ataatgtggt cttttgatat agttcataat agttacctca aagatagtat aatacaaata
     2161 gatgttattt tcaaatattt agaaataatg tttaatttat ttaattattt ctaattgttt
     2221 agaaataata tctggaacta tttaaatatc ttcaagatag aaaaagaaat tgtacttgtt
     2281 gatgttgaat catttgtgtt aacaagtcat ttagaagcat ttaagaaatt agtgaaatga
     2341 aaaactcatg gcagaacaaa ttgttgggta tatggtctat tactaacaac tactgctagt
     2401 ttatgagata gatatttaat tattctcgga taaataggat aagcgtgctg aaaacttaga
     2461 tatgacaaac aaaataaaat ttttaaaagc tacagagaaa catggtataa tgtatctaaa
     2521 ggaggtagca tatgtcctta gatatagata aagaaaagat gacaatcatg ggaatagctt
     2581 ttgaaaaccg atccgttttt aagagtgttt ggtatgcttt aagtacaaat atgattgaag
     2641 gatggcgacc tacggttagt gatgttgaaa aattacgaga tgaagctctt gctctgggga
     2701 tgacttaatg aagcgcaact gttatgaatc ttatgactat gttgatccgg ataatttata
     2761 taccgatccg ggttcttcag ttctaagaaa taagcaggaa ggaaaaaagc acgtgagctt
     2821 gaatatcgta tgattgctag taagagtttg aaattattta tcaatcctat cttagttaat
     2881 agtttaaaag tagtttgttt cacaactgca taagcttaga aaagttgaaa agtgataact
     2941 ctttaataaa ctcttggttt gttaaaagat attttgtcca ctgcattttt tcaattgact
     3001 attatttgtc atagatataa cagatagaac attgcattca gaataatgaa aaacgcatca
     3061 aaaatggtgc gttctgtttt tatgctacca taaaaggagg aaaacaaatt gaaaattttt
     3121 aagggagagt tttatcgaat ctctgtatta acagacaagc tagtaaggtt agaatactct
     3181 cgaactggaa attttgagga tagaacgaca caacttatct ataatagaga ttttggccaa
     3241 atttcgttag attatatcga gacatcaaac gtactagata ttatgacgga ctattttcat
     3301 ctgcacttta ataaaggaga atttaacgcc gaaaatttat ttatagaatt aaaaggaaat
     3361 tttgccgtat atggtagtcg ctggtatttt ggtgaatcta ttgaaacgtt aaaaggaaca
     3421 gctcggactc tggataaggc agatggagca atctcgttag aagatggaat tattagccga
     3481 aatggtatag ccttattgga tgattctcaa ggatttattt gggatgaaca atctggttat
     3541 attgagagag aaaatcaaat tgacctgtat ttctttgcct atgggcatga ttatagagga
     3601 gcaatcagag acttttacca tttgactggt tcaacaccct tgttgccaag atatgcttta
     3661 ggcaattggt ggagtagata ttggccttat acgtcggatg aatacttgga tttaatagac
     3721 agatttgaaa cagagaaaat tccattatct atcggtgtgt tagatatgga ttggcatata
     3781 actgacattc cagctcgttt tggaagtggc tggacaggat atagttggaa tagaaactta
     3841 ataccaaatc cagaacagtt attgcaacaa cttcatgata gaaagttaaa actctcctta
     3901 aatgtccatc ctgctgatgg gatacgggct tatgaagaag cttatcctca aatcgcaaaa
     3961 cgattggggt taaatgtaga actagaagaa cctgctattt ttgatttttt taatccctct
     4021 tttagggaag cctactttaa agatgttcat tatgagctag aaaagcaggg agtagatttt
     4081 tggtggattg actggcaaca agggacacaa ggcatgctag atccactttg gcttttaaac
     4141 cattatcact atcaggatag ttgtaaaaat gcagaaggtg gtttgatttt atcaagatat
     4201 gcaggtcctg gtagtcaccg ctaccctgtt ggtttttcag gggatactat tattagttgg
     4261 aattccttaa gatttcaacc ctattttaca gcgacagcat ctaatatcgg ttatagttgg
     4321 tggagtcatg atatcggtgg acatatgttg ggggattatg acgaagagct acaaactaga
     4381 tggctacagt ttggtgtttt tagtccgatt actcgattac atagttctag aagtcctttt
     4441 aatagtaaag aaccttggtt tttttcagaa acaacatcta agattatgaa gaaatacctt
     4501 cgtttgagac atcagatgat tccttatcta tataccatga atgtaaagac acatgaggaa
     4561 ggtgccccat taatcagtcc aatctattat ttctatccag agaatgatga gagctataat
     4621 gttccaaacc aatacttttt tggaacagaa ctgatggtgg ctcccattgt agaaaagatg
     4681 gatttgacat tccaatctgc aaaagtagat gtatggttcc ctgaaggtga atggtatgac
     4741 ttcttttcag agaaaaaata cacaggtggt gtgaagttaa gtgtttatag ggacatctcg
     4801 actacgcctg tgtttgcaaa aagtggtgca atcattccct tggttggttc tgagataggt
     4861 atgggtgttg atttacctga ggttgtagat tggtatgtat tcccagggaa acaacattct
     4921 tttgaaatgc ttgaagatca aaatggtcaa agatataaaa caagattatc aatcgattgg
     4981 gaaatgggaa tggtagagtt agcattacaa ggagattcta gtatcgttcc aagcaataga
     5041 aaacatagaa ttcattttaa aggaacgaat gtgtctataa ttgaattgcc aaataagaat
     5101 gatacagcta aatttgaatg gaaagataat aaaaggacat ctttaaatga cgaagttttt
     5161 agactactaa agacagcttc tcttccatat gaattaaaag atagattgtt aaatcaattc
     5221 atcaatgcca aaaactctca tgacttaatg aatatcttgc atcatcagga taaggaattg
     5281 agagggcgtt tgttggaaat gatatttact agccaaaact aattgaaata attagaaagt
     5341 catttgttta gtgtatatga agattctgta tggtatttgt tatcattggt cacattgaaa
     5401 atgtgaaata agcacctaca acatagactt tacaacactt catgtttcat atcatggggt
     5461 gtttttctat ttaagtcaaa gccaatcaaa aaatctcact attcatcagg ttaaaggaaa
     5521 cctaatcata gtgagattgt gaattatagt agagtttgta tcgcctcttc aatttgtttt
     5581 gggtctgttt ttgaagaaaa gcgttcaaag acttgcccat ctcgaccgat gagaaactta
     5641 gcgaaattcc attcgattcg ttttcctagt gggccagatt tctggtcttt taaccagaca
     5701 tagagagggt ctgcttcctt accgttgacc ttaatcttgg caaaacgtgg gaaggtggtt
     5761 tgaaaatgta ggctacagaa ggcgttgatt tcctctgcgc tgccgggtgc ttgtcccata
     5821 aactgattgc aagggaaatc caatatttcg aagccctgat cttgatagcg ttcatagagt
     5881 tcttgaagtc cctggtactg gggcgttaaa ccacatccag tagcagtgtt gacaatcaag
     5941 agaactttgc cacgatagct atccaaggga gttgcttggt tgttttggtt caaaacggaa
     6001 aaatcataaa gtgaagtcat gagggcctct tttctttttc tacattgtaa cagtttttac
     6061 ttttttcgtc gagtcggatg gaagatattg ttgaagaata agccctcaat atgatagaaa
     6121 ccgtttacaa ataactttaa aaatggtata atttaattaa accggtttac ttagttgcta
     6181 taaatctcta atttgagatt tagttcctat tgattttaca atatgtttat tggagtgtat
     6241 acatgcaaac aaaaacaaag aagctcattg tgagtttgtc ttcacttgtt ttatcaggat
     6301 ttttattaaa ccattatatg acagttggag cggaagaaac gactacgaat accattcagc
     6361 aaagccagaa ggaagttcag tatcagcaaa gggatacaaa aaatttagtt gaaaatggtg
     6421 attttggtca gacggaggac ggaagcagtc cgtggacagg aagcaaagct caggggtggt
     6481 cagcttgggt agaccagaag aatagttctg cagatgcctc aactcgagtc attgagacta
     6541 aggatgggac tatcactatc tcaagccctg agaaattaag ggcagcgctt caccgtatgg
     6601 ttcctattga agctaagaaa aagtataaac tgcgtttcaa gattaaaaca gataataaaa
     6661 tcgggattgc caaagttcgt atcattgagg aaagtggtaa ggacaagcga ttgtggaatt
     6721 ctgcaacgac gtcaggaaca aaggactggc agaccattga agcagactat agcccgactt
     6781 tagatgttga taaaatcaag ctggagttat tctatgaaac aggaactggg actgtttcct
     6841 ttaaggatat tgagctggta gaggtagcag accagctttc tgaggattct caaacagata
     6901 aacagcttga ggaaaagatt gatttaccaa ttggaaaaaa acatgttttt tctcttgcgg
     6961 actatactta taaggtagaa aatcctgacg ttgcttcagt caaaaatgga attttagaac
     7021 ctcttaagga agggacaacc aatgtcattg tcagtaaaga tggcaaggaa gtgaaaaaga
     7081 ttcctttgaa gattctggcc tctgttaagg atgcatacac agaccgtttg gatgactgga
     7141 atggcatcat cgctgggaat caatactatg attctaaaaa tgaacagatg gccaaattaa
     7201 accaggaatt ggaaggaaag gtagctgata gcctatccag tatttcaagt caggcggacc
     7261 gcacctattt gtgggaaaaa ttttcaaatt ataaaacgtc tgcaaatctg actgccactt
     7321 atcggaaatt ggaggagatg gccaagcaag tgaccaatcc ttcttctcgt tattatcaag
     7381 atgaaactgt cgttcgaaca gtcagggatt ccatggaatg gctgcataaa catgtctaca
     7441 atagtgaaaa gagcattgtt gggaactggt gggattatga gatcggtaca cctcgtgcca
     7501 tcaacaatac cttgtctctg atgaaagaat acttctctga tgaggaaatt aaaaaatata
     7561 cagatgtaat tgaaaaattt gtaccagacc ccgaacattt ccgaaagacg actgataacc
     7621 catttaaggc tctaggtgga aacttagttg atatgggaag agtaaaagta atagctggtt
     7681 tactgcgtaa agatgatcaa gaaatttctt ctaccattcg ctcgattgag caagtgttca
     7741 agttggtaga ccaaggtgaa ggtttttatc aagatggatc ctatatcgac cacaccaatg
     7801 ttgcctatac gggtgcttat gggaatgttt tgattgatgg cctgtctcaa ctgttgccag
     7861 tcattcaaaa gaccaagagt ccaatcgata aagataaaat gcaaaccatg taccactgga
     7921 ttgataaatc gtttgctcct ttgctggtga atggagaact gatggatatg agtcgtggac
     7981 gctcgatcag tcgtgcaaat agcgaggggc acgtggccgc agtagaagta ctaagaggga
     8041 ttcaccgaat agcggatatg tctgaaggag aaaccaaaca acgtttgcag agtcttgtga
     8101 agaccattgt tcaatcggat agttattatg atgtctttaa gaatttgaag acttataagg
     8161 atatcagttt gatgcaatcc ttgttaagtg atgcaggagt cgcaagtgtt ccaagaccaa
     8221 gttacctatc tgcctttaac aagatggata aaacagccat gtacaatgca gagaaagggt
     8281 ttggatttgg cttgtcactc ttttccagtc gtaccttgaa ttacgaacac atgaacaagg
     8341 aaaataaacg tggttggtat acgagtgatg ggatgttcta tctttacaat ggcgatttga
     8401 gtcactatag cgatggctac tggccaacag ttaatccata taagatgcct ggtacaacag
     8461 agacggatgc taagagagcg gatagcgata caggtaaagt tttaccgtct gctttcgttg
     8521 gaacgagcaa actagatgat gccaatgcga cagcaaccat ggatttcacc aactggaatc
     8581 aaacattgac tgctcataag agctggttta tgctaaagga taagattgcc tttttaggaa
     8641 gcaatatcca aaacacttca acagatactg ctgcaactac aatcgaccag agaaaactgg
     8701 aatcaagtaa tccatataaa gtctatgtca atgataaaga agcctccctt acagaacaag
     8761 aaaaggatta tcctgaaacc caaagtgtct ttttagaatc gtccgattcg aaaaagaata
     8821 ttggttactt tttctttaag aagagttcaa tcagcatgag taaggctttg caaaagggag
     8881 cctggaaggg tatcaatgaa ggacagtcag acaaggaagt tgaaaatgaa tttcttacga
     8941 ttagtcaggc tcataagcaa aatggagatt cttatggcta tatgctcatt cctaacgtgg
     9001 atcgtgccac cttcaatcaa atgataaaag agttagaaag tagcctcatc gaaaataacg
     9061 aaacccttca gtctgtttat gatgctaaac aaggagtttg gggcattgtg aaatatgatg
     9121 attctgtctc tactatttcc aaccaattcc aagttttgaa acgtggagtc tataccattc
     9181 gaaaagaagg ggatgaatat aagattgcct actataatcc tgaaacccag gaatcagctc
     9241 cagatcagga agtctttaaa aagctagagc aagcagctca gccacaagta cagaattcaa
     9301 aagaaaagga aaaatctgaa gaggaaaaga accattcgga tcaaaagaat ctccctcaga
     9361 caggagaagg tcagtcaatc ttggcaagtc tagggttctt gctacttggg gcattttatc
     9421 tattccgtag aggaaagaac aactaatttg ttcatcatct agatgaatta catgaaactg
     9481 tggaaaatag aaaaaatccg aagaaggcca aatatcttcg gatttttctt ttaaaaaagg
     9541 ataagttgat acttatcctt ttttacttca ttattatgtg actatagcgt tttgaaacta
     9601 gaatagaacg ttataactgc taaaagattt ctataaattc atttgatttt cctaatcaat
     9661 ttgttcgtat cctatttcac tccactatat aagtccgtct cgctccgtga ggtgcgcgtc
     9721 gaaggttata ccaaaaaaac tccaaacgcg atacaataaa ggtattcaat ccaattgtaa
     9781 agcgaaagga gaaaaaatat ggcacaaaag actcatagtt tatcatacac aaagtggatg
     9841 tgttctatca tattgtgttc acccctaagt atagacgaaa agtcatctat aatcaatata
     9901 gaagtagttt aggcgaaata tttcatcgct tgtgtagtta taaaggtgtt gaaatgatcg
     9961 agggtcactt aatgccagac catgtacaca tgttagtcag tattccgtcg aggctaagtg
    10021 tttcaagttt tatggggtat ttaaaaggca aaagtgcact catgatgttt gacaaacacg
    10081 ccaatctcaa gtacaagttt gggaatcgga atttctgggc ggaaggttat tatgtaagtc
    10141 cagtagggct taatgaagcc acaattaaga aatatagtca agattaaaga aattatcgag
    10201 tggatgattt cttcacgagt atgaaaattt gagaactagt aaagtatgat atatagtaaa
    10261 atgaaataag aacaggacga aatcgatcag gacagtcaaa tcgattttta acaatgtttt
    10321 agaagtagag gtatactatt ccagtttcaa tctactatag cactagataa attgagtgta
    10381 aaagaatatg aggatccctt tagggatagt ggtaagtaat accaaagtct cttaaagagg
    10441 caagtgacga gtcaagagca ataaggcttg aacaacgtga aagccagcgt ctttaggcgc
    10501 tggctgatga tttgggctta tagctctgag ataaaccacc cgttagacgg gtagttatga
    10561 ttttatctga gtgtaatata ctgttgggca atctcgctga tgcggtcaaa gttgccttgg
    10621 gaagcgagtt tattgagttc gccaccaatt ccaacggcat ctgcaccagc agcgaaccat
    10681 tgagggatgt tgtttaggcc gactcctccg gttaccatta cggaaacttg tgggatcggt
    10741 gccttgactg cagagatata tgctggactg agagtactac ctgggaagag tttgatgatt
    10801 tcactaccgg cttcaagtgc agtcgtgatc tctgtgaggg taatacagcc tggaatgtac
    10861 ggtgtgctgt agagattgca cattttcgca gtttcagcat ggaaagatgg agaaacaacg
    10921 taatttgctc cagctagaat ggcatctcta gcagttacgg catcaagcac agtacctgca
    10981 ccgatacaaa cactctgatc gtcctgatac aagtctacaa gttccttgat gatttgtcct
    11041 gcatactgat tggtataggc gatttcaata gctttgatac cgcccttgat acaagcaatc
    11101 gaggcttgta gtccttcttc ctttgtattt cctcgaatga cagcgacaat tttctgtttt
    11161 tttagttcaa taatcgtatc tgatttggtc atgtaattct cctaacgaat gatatcttgt
    11221 gcatttgcca gtaaattttc aatactagtt gcggaagtgg agagatggtc tcctggaaga
    11281 gtgcatttga gagttgcgct cgcaactgca aagtcaatgg tagtttttag ggaggaatga
    11341 tggagtagtt ggtaaagggc accagatata aaggcatccc cgctaccaat tcgttgatag
    11401 actgcagttt ttagttggac agactcttca aatcgttctt ccagagcata ggcttgatag
    11461 acatttttgt cttgctcatc actagatcgg agggtatgga aaatagcctt gaaaccatag
    11521 gtttctttta aaagtcgcat gcgattttcc acctcttcta ggctagcact gtctcttgga
    11581 aacatctcta gattttggtc atcaatcatg agaggatcaa taccgaagca atagtcagta
    11641 aaacgtgcaa acttagaaaa ttcatacttg gcttctaggg ctgaaatcat ctttgttctc
    11701 agattgagat ccattgaaac gacaattcct cggcgcttgg cttcttccaa gagtaggaga
    11761 aggatcgcac ggacctcttg accgatagct atggtgattc cactaaaatg aaaatggcta
    11821 atcccctgaa agagagaatc catatctagc atgtttggcc gaatctggct gatactcgta
    11881 tgcttacgat cgtagaaaac ttcactttga cgacaaccaa agccgttctc caaatagtag
    11941 aggccgattc gatcgccaag ccgacagatt gaactggtat cgatttggtg ctgtttcaag
    12001 aatgtgagaa aacgatctcc aatctcgttg gcagggagtg cggtaaagac tttcgttgag
    12061 atacccaggg cttgcaaatt acaagcgatg ttaatttctg atccgccaaa ataagtcgaa
    12121 ctggcaacat gatcgccgat actggtggca tctaatggtg agattcgaat taatggttcg
    12181 ccaaacagta atattttcgt catccattag ttcctttctg gtttagaata gctttacttg
    12241 gctaatactt ctttcaaata agcagcgact tcatcatctt ggcagttttc aaagaagtat
    12301 tcttggaagt atttgccaga gatggtgtct ttgaggaagt cttggtcgat tgttttaagg
    12361 atggtcatca aatcattgtg ggtgatttgt ttcacctcgt ttaagatacg agcgttgcgt
    12421 tgttcaggga ttacacgttc tcttgggtag ccaccgccca tttcttcagc aaataagcgt
    12481 tcaaacatca atttgagggt cagttctgcc ccccagccaa atcctttggc ataagggata
    12541 gacaaggcgt taccaccatt gatttgagaa taaaggtaag cgtcagttgg gtccactgct
    12601 agaccacaga caacaccagg gaagctgttt aaagcaagca tagcccctac acccgtacca
    12661 cagccggtaa caacaaagtc aactgccttt gtatttaaaa ggatggcagc cattagtccg
    12721 ttctgcacat aagttaattg actttctcct tcttctccac gcataccata gttaaataat
    12781 tggtagtctt ttttatctgt cgcttctttt agactatcgt aaataatgtg attcttgcta
    12841 gcttgactat tttcattgat taatgcgatt ttcataagat cttctttcta tttttactca
    12901 ggttgttttc cgatgtaggc taagataccg ccatctacat ataggatgtg gccattgaca
    12961 aaattgctgg catcactagc gagaaagaca gcagggccca tcaaatcttc aggatttccc
    13021 caacgtgcag caggtgtttt tgcaatgatg aactggtcaa atgggtggcg agaaccatct
    13081 tcttgcaact cacgaagagg tgctgtttga ggagtggcaa tataacctgg tccaattccg
    13141 ttacattgga tattggctcc accgtattca gacgcaatgt tgcgggtcaa cattttcaag
    13201 ccccctttag cagcagcata agcgctaact gtttcacgtc ccagttcgct catcatcgaa
    13261 caaatattga taatctttcc atgccctttc tttatcatag aaggaataac tgcctttgaa
    13321 acgataaatg gtgcgtttaa gtcaatatcg atgaccttac ggaaatcagc ggcgctcatt
    13381 tcgcacattg gaactcggcg gataatacca gcgttattaa cgaggatgtc aatgacacca
    13441 acctcttgtt cgatttgctt gaccatggct tggataccgt cctcgtctgt cacgtcacag
    13501 acatatccat gggcttggat gccaacttca cgataagctt caatcccttt attgaccaga
    13561 tcttgattga tatcgttaaa gacaatagta gcgccggctt cagcgtagga tttggcaata
    13621 gcaaatccaa ttccataaga agcgccggtg atgagagcaa tttttccttt taaagaaaac
    13681 tgctcaattg agaatgatgt atttgtcatt tgatacttcc tttcaaat
//
LOCUS       DAVLTM010000049        13502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLTM010000049 DAVLTM010000000
VERSION     DAVLTM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.050"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..1433)
                     /gene="pfbA"
                     /locus_tag="VYN16_001220"
     CDS             complement(<1..1433)
                     /gene="pfbA"
                     /locus_tag="VYN16_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1403751.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNNISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAK"
     gene            complement(2597..3823)
                     /locus_tag="VYN16_001221"
     CDS             complement(2597..3823)
                     /locus_tag="VYN16_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1403752.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWRANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3866..4558)
                     /locus_tag="VYN16_001222"
     CDS             complement(3866..4558)
                     /locus_tag="VYN16_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1403753.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMHYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4731..6482)
                     /locus_tag="VYN16_001223"
     CDS             complement(4731..6482)
                     /locus_tag="VYN16_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403754.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGLSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(6472..8217)
                     /locus_tag="VYN16_001224"
     CDS             complement(6472..8217)
                     /locus_tag="VYN16_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1403755.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     CLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8423..8719)
                     /locus_tag="VYN16_001225"
     CDS             complement(8423..8719)
                     /locus_tag="VYN16_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1403756.1"
                     /translation="MSASDVALKLRGQGIFQMKQVKRAVQEQNGQLIVVQMGDENSKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8872..9327)
                     /locus_tag="VYN16_001226"
                     /pseudo
     CDS             complement(8872..9327)
                     /locus_tag="VYN16_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9460..10287
                     /gene="xth"
                     /locus_tag="VYN16_001227"
     CDS             9460..10287
                     /gene="xth"
                     /locus_tag="VYN16_001227"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1403757.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10299..10442
                     /locus_tag="VYN16_001228"
     CDS             10299..10442
                     /locus_tag="VYN16_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1403758.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCDLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            10442..10681
                     /locus_tag="VYN16_001229"
     CDS             10442..10681
                     /locus_tag="VYN16_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1403759.1"
                     /translation="MLEEGTKDQLAELTYLFGRGVNLSFGIKDVSKLYQRVMKANYPI
                     YRPLTKRKFRVSDHYIYPHEFAVLDPDSYFLRFSE"
     regulatory      11065..11160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11560..12141
                     /locus_tag="VYN16_001230"
     CDS             11560..12141
                     /locus_tag="VYN16_001230"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1403760.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            12141..13403
                     /locus_tag="VYN16_001231"
     CDS             12141..13403
                     /locus_tag="VYN16_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1403761.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
BASE COUNT         3999 a         2908 c         2618 g         3977 t
ORIGIN      
        1 tcttttgcaa ctcgtatcgc ttttgtttta ggatcggcaa tattaaatat attttcggcg
       61 ataaccacct gtttatttaa atctaatagg tctttagtgt ttttatagct ataagcattt
      121 actaaagctg ctccgttttc tcgataatgt acactctctc ctttaacttt cttatcaaag
      181 cgatttcctt tgattaatac atcagtgaat cctgtaaaac gtacacctgc atacatcatg
      241 ttatcaaaat gattatttaa aattttaata ttagaggggt tctgtgttga caatgtttga
      301 tagtgtgtgc caattgctgt tactaattcc ccagatttat cacttttgcc aaaataggaa
      361 ttttgaatag tcacattttc agattttttc ccatcatcat tcaaggcata aggaaaacct
      421 tttctagtta atggttcaat ctgaatgctc tccttactta tgatttgccc atccttcatc
      481 gttttgggta aggcttgccc aagaaaacga gaattatcaa ctaatacatt tttcgaacct
      541 gcaatttgaa tagcatgccc ttgataacta tccttgaatg ttacattttt tatagttacg
      601 ttatttgaat tcccaatagc aaatgcacct gaagaattta taagtggtag attttttgct
      661 ttagttcctt cttcattcaa agcaccgttc atatcaatcg taccaccaga ataactaata
      721 tcttctgttg ggccccattc tacttgcgca ccatcatccg taaataaacc tgtcataaaa
      781 acaatggaag ggtgattctt aatatttata ccatttagaa ttgtagcttt ctcattcaat
      841 tctaagtgtg tatgactttt taaaaaaaca atttctttta ctaaataagt tccttcagga
      901 aaatatacat ttcctccacc tagcccttga gctgcagcat ctattgcatc ttgaattgct
      961 tgtcgatcat catgaatccc atcacctact gcaccataat cttttacact taaagcagta
     1021 tcttcaaatt cttttttgat aattctgtca cttataacat cttccttata actcttattt
     1081 tcattagtag atgttactgt agattcaact gttctgttgt ctaattgtga attggaacga
     1141 atagtaccat tagagctgat attattaaca cttaacgaat tatctaaacc attagtggtt
     1201 gaattagagg caatcatttc agcagaacga ttctgattaa cagttggtga attatctaaa
     1261 tcattagtaa ttgcattaga agactctttt gtagccatcg gtgaagaact agtaacaact
     1321 tcatccgata aaataccttg gcttcccaaa attgaaattg ccaatagtac cgagcaagtc
     1381 cccaccttta atttacgaat actgaatacc tcattaggaa caaaatattt catcagaacc
     1441 tccaattttt ttactatttt taacaattga ttattacttt tcttaaaata gatagaatga
     1501 caccgctgtc attatcttta tcatatattt tccaaattga attgtcaagg aataacacaa
     1561 gaaaatatta aaatttatac tcttcggaaa tctcttcaaa ctaggtcagt gccgccttgc
     1621 cctaagtcta ggttactgac ttggtcagtt ctatagtaga ctgaaactat aatagtacac
     1681 ctctacttct aaaacattgt tagaaatcga tttgactgtc ccgatcgatt tgtcctattc
     1741 ttatttcctt ttactatatc tacaacctca aagcagggtt ttgagctatc tatggccaac
     1801 ttcctagttt gctctttagc cttccattca aaagtgatgc tatctttctt agcttgaata
     1861 taaaatatcc catttactat atttaaattt attgatatgt tgtaaattat aaatgaaaga
     1921 acaaatacct tgtcatacaa gcattcgcca gggatattta catgaatatc gctcatatgg
     1981 cggattgaaa ctaaaacact acaatatatt tcttaaatag accttctata aaagctagat
     2041 ttaacctcga taaaaataga tgcagtgctt ctaaaatact ggaatagggc atataggtaa
     2101 tagtagacaa gtgttttata gattgtctcg caggagatct aatgatgtat agcaagcatc
     2161 actaaacata tagaatattt gcattattat aatatcaaca tgttataaat tctatcatgc
     2221 aaaagaatga ctagaacatg aaagacaggc acaaaacttt gaacaatatt ctgaaaacaa
     2281 aaaaaagacg cgagtttcca caatctcact catggaaact cacgtctttc aaaaaatagg
     2341 gtgaattgaa aactagaaca agacactaga ttttctaaat gatgtctagt aaggattcta
     2401 aatgctacac caaattgctc acatcttatt tcaaaccacg ggactcccac aagaaaggaa
     2461 aatcctataa aaattctctc tctgaggaga aagaggcctc aactacaggt gtaaactatt
     2521 atcaattgaa aacaacagtt tttacactca attagacttt ttagggacag gatcactttc
     2581 gcttgactgt agtttgtcat ttttttgata aagttagcac tttttcagaa actgttttga
     2641 aagtctcaat gatatagtct acttcttcat cgcttaattt agtatgaaga gggagggtaa
     2701 tttcattctc aaagaaggca taggctttag gatagttcgt catatcaaat ccaagattct
     2761 tataggctgt caagagagga agcggtttgt agtgaacatt acttgcaatt cctgctttag
     2821 ccaattcttg gatgatgagg ttgcgttctt ctaggcttgc tccttctaca cgggtgatgt
     2881 agaggtggcg tgaagattcg acagtttcag tcttgtgtgc caaaggatgg atgcgagaac
     2941 ctgcaaaacc actatcatag cggtccacaa tgtccttacg gcgttgcaac aaacttggat
     3001 agcggtccaa ttgtaccaaa ccaagtgaag ccatgatatc ggtcatgttg cacttatagg
     3061 ctggtgtaac gatatcgtat tcccatgacc ccagttgcat cttggcaaga gcatccttag
     3121 tttgcccgtg aagggaaagg atttggaatt ccttgtacat ctcttcgtca tcaatcactg
     3181 gattggctct ccaagtcgca cttccacctt ctgccgttgt aaagttctta acggcatgga
     3241 atgagaagga agtaaagtca gcgatagaac cagaaggttg tcctttataa gtagatccca
     3301 aagcgtgggc actatcagag acaatgacaa tacggttaaa ggccttttgc cacttgcttg
     3361 aagcagtaaa gaagtcacgt tttttctcca cgacttggaa caaacggtca taatcgcaaa
     3421 caatccctgc gagctctact gggataatca ccttagtttt ctcagtgata gcttgctcaa
     3481 gcaggtcata gtccatctca aacgtatctg cttggatatc caccatgaca ggggttgctc
     3541 ccacgtgcgt aatgacacta catgaagccg tataggtcat ggctggaacg atgacttcat
     3601 caccaggtcc cacttccaaa acgcgtaaaa tcaactccag agcggctgtc gcagagttga
     3661 gacaaacagt cttaggtgtc tgtgtgtaaa gagacaagcg gcgctccagt tcttttgttt
     3721 taggacctgt tgtgatccaa ccagaacgca gggtatccgc tacttcagca atttctgctt
     3781 ctgtgatatc aggcggtgaa aatggaatat tgtaatttgg cattgatttg ctccttttat
     3841 ctgtactcat tttcttatga ctactttatt ttagtacctc aaacacggtt tgaaacatga
     3901 ttttgatgtc cccaaagaaa ctaaactctc ggagataggc gaggttatag tgcatctttt
     3961 caggaagaac atgctccaca taggcctgat caactgacag acctttctcc gtcatttgac
     4021 tgatgatggt gtcctcatcc ttgtagttga tgctggctgg agaggtaatc cctgcttgca
     4081 agagcaaggt tgccatcatt tcagggctat actgctctgt ataacgtggc acttcaggtc
     4141 gtgtaccgac aaaggacatc tcacctttaa ggacattgac caactgaggc agttcgtcca
     4201 aacggacacg tcggatgaaa tttccaacct tggtaatgcg gctatcgtta gcagaagtca
     4261 ccagacttcc ttttttatcc gcatccgtca ccatggtacg aaacttccaa atcttgaacc
     4321 gacggttgta ctgggtcaca cgctcttgct tgtaaatcac tggccctttg ctatccaact
     4381 tgatccaaat gctcaagatg agaaagatgg gagaggtcag aaccagtaag accaaggcca
     4441 aaacccagtc caagcaacgc ttgaaaatca gcgaaccctt ccttttagag acaagctggt
     4501 agtaagactc aacctcgctt gatttcattt ccacaggcaa gtcttcccat ttcagcatgc
     4561 tgttctcctt tgtttttatt atactatttg tcaacatttt tcattatacc acaaaatgga
     4621 aaaggcggtg aaagaaatct gtaatttgaa ggatttcctt ctagtactca atgaaaatca
     4681 aagtacagct ttgagattgc agataaagct gacatggttt aaagagattt tcaaagagta
     4741 tcggccatgg cccctgcctg caaactatac atcttgtgat aggttcctcc cagagccaag
     4801 agttcctcat gggttccact ctcgataatg cgtcccttat ccaagacata gatgcagttg
     4861 gcatcttgaa tagtagaaag gcggtgagcg atagcaatag ttgttcggcc ctgtctcatc
     4921 ttcgccagag aagcttgaac caagctttct gtttcagagt caatattggc tgtcgcttca
     4981 tccaaaatca ggattttagg ctggctggcg actgttctag caaaggcaag aagctggcgt
     5041 tgcccagtag agaagctcaa accacgctcg gaaacagggg agtcgtaccc ctgaggaagt
     5101 tcttgaataa aggaatctgc atccacaaag gctgccgcag cctgaacctg ctcatcactg
     5161 gtttcttggt acatggcgat attggactta atagttccat gatagaggaa gggttcctgc
     5221 aagaccaaac cgatgttttt tctcagctct tcttgactga aatccctgat atccacatca
     5281 tccaagagaa ctctccctga ctggaattca taaaagcgca tgaggacatt gataatagac
     5341 gatttccctg aacctgtatg acctacaaag gcaatggttt cacccttatt aacagagaaa
     5401 gaaatgtcat ccagaatcgg atgtttaccg tcatatgaga aacacacatg ttcaaaacgg
     5461 atattgcctt cttggacttt ggcttgccca ttttcttgaa gaggttcata ggtcctctcg
     5521 tctatcaggg caaagacacg acctgcagaa accatagccg tttgcagagt tgaaaagttt
     5581 tgcgtcacct caatcaaggg gtcaaaaagg cggttgatgt actggataaa ggcatacatg
     5641 gtcccgaccg ttatcccgat agaaaaacca cggtagccaa agtaggccat caagactgca
     5701 tagcctagaa gtttcagcaa actcatggca ggtctcaaaa agagggcatc caaggctaca
     5761 gaacggttgg cgtagaccaa gtgttcttgg ttgatttcat caaattctgc ctgcaggcgc
     5821 ttctcttgat taaaagcctg aataatcctg attccctcga tattctctgc cagcttacta
     5881 ttgatatctg acaagagact tctggttttc tcgatgattt tcactgactt ttttcgatag
     5941 agattgacca aaaggaaaat caaagggaga aagagcaaga ctaaagccgt caaacgaaaa
     6001 tccagcacca acatggtata aagggttgtc agaaagataa aaactgctga gataaagctg
     6061 gataaaatcc cagaaaacat atcactaatc gtctcggtat cgttggtcaa acgagaaacg
     6121 atagaacctg ctggcgtctt gtcaaagtaa gacatgccca gtttctccat attggcaaag
     6181 gcatcccgac gaatatccct aacaatactg taagacacgc gcgcaaagag aagattgcct
     6241 acatactgaa ctacagtttg taggatgtag agaccatagt agaccagcaa aacggtaacg
     6301 gctagttggt taagattgct gagatactgg tcgataaagt gggaagccac gaggggtatg
     6361 acacttttaa tgaccgtcgt cgctaggaga aaactgagtg ccaaaaaggt caggagtcta
     6421 taaggcttga gataagacat caagcgcttc aatacagccc attgttcttg tttattctgc
     6481 atcttcttct cctttcattt ctaactgctg agactggtag gtttgggcat accagccatc
     6541 catcgctagc aagtcttcgt gcctgcctcg ttcgataatt tgaccatttt gtagaactaa
     6601 aataaaatct gcatggacaa cagcactgag gcgatgggca gtgataatgg ttgtcttgtc
     6661 ctttcgcgtc tccttgaggt tgtcgataat cgcatactct gtcttggcat ctacggcgga
     6721 taaggaatca tccaaaatca agatatcagg gtctaaaatc atagcccgac tcatagccaa
     6781 ccgttgcttt tgaccaccag aaagactgac tcctttttca ccaatcagcg tatcaaatcc
     6841 ttgaggcatg tctacaatat cttggtaaac tcgggctagc ttagtagctt cctcgaccgc
     6901 tgaaaggggc aagttaggat tgccaaagcg gatattgtct aggattgaag ttgcaaagag
     6961 aaactggtcc tgaggaacat agcccatgag actgcgaagg tctgtcagac gatagtcccg
     7021 aatatcgtga ccgtttagat aaatggcacc cttatccaca tcgtattcac gcaagaggag
     7081 cttgattaag gacgttttcc cagagcctgt ctgcccaacc aagcccagtg tttgcccttt
     7141 tgccaaacta aagtgaatat ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat
     7201 ggcatactcc aaacacccat tttcaatacc atccagagga aactcagggt cttgtacagg
     7261 agattcctga gacaaaagat tttcaatccg ctggtaagaa accttccctc gctgagtagt
     7321 attaaagagg aagccgatgg ccataagagg ccagaccagc atatccaaat agctgataaa
     7381 ggtgactaga ttcccaactg taatctgccc ttcctgaacc atcaaggagc caaccaaaag
     7441 cgttaaaaca taggacgaac caacaaacac gagaaccata gggtcaaaga gactatcata
     7501 tttcatggtt tgcaggttct tttggaaggt taattcattg actgcctgaa aagacttcaa
     7561 ctcgtctgcc tgataaccga aagacttggt cactttgata cctgatacgg actcctgtac
     7621 cttgttattg agttcagaaa aagcagcttg ggattcgcca aaggccttat gagtctttct
     7681 ccctaggcga ctagtcgcat aggccatgaa aggtaggggg agaatggcaa caagagtcat
     7741 ctgccatgag atgctaaaga gcatggtcaa caaagtcacc agagccgtga tagaggcatc
     7801 caccgcagac atgacaccgc cacctgctaa acgagtcaag gcattgatat cattggttgc
     7861 gtgtgccatc agatcacccg tccgataggt ttgataaaag gctgatgaca tttttgtgaa
     7921 atgcttaaac aagcgagacc gcatgatctg tcccaagcaa taagaggtcc caaggatata
     7981 catacgccac acatagcgca aatagtacat accaaaggct gcaagtagca agtaaaatag
     8041 gctaagaagg aggtcctgct gggttaattg ccccgatgtg atggcatcaa tgacccgccc
     8101 cataaccata ggaggaatga gattgagaac ggaaaccaag accagggcca caatcccgac
     8161 tagataacgg cgtttttcta acttgaaaaa ccaccaaagt ttttgaataa tggacataaa
     8221 atccctttct gattgcaaat ggaaacctga agccaatact caatgaaaat caaagagcaa
     8281 actaggaaac tagccgcagg ctgctcaaag cactgctttg aggttgtaga tagaactgac
     8341 gaagtcagta acctacatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag
     8401 agtacaagac cccaggtttt tcttattcat aagttacgac tgtaacagca cccttgtcat
     8461 attcagcaat aaagatattg gctacattgt catgcccttg tttactgagg ttatcaagca
     8521 accactcttc gctacgacca atcgattcca agacatctac ttgaatcaca ccgtcagtca
     8581 caactggata cttagaattt tcatctccca tttgcacaac gatgagttgc ccattttgct
     8641 cttgcacagc tcgtttgact tgcttcatct ggaaaatccc ttggccacga agtttgaggg
     8701 ctacatccga tgcagacaaa ccaactgaac gacaggctta tgggtcaata ttcccatttt
     8761 tgataaggag agttggctta ccatcaatca aacgtttcac aaagtgaaca ttgttgttga
     8821 gctatttgag agtcaagacc agtttgatta aaatttcgat ataattaagt gtcatttgtt
     8881 cacatccacc agttcaattg cgtctgtctt atacaattcg actttctcta acaggtactt
     8941 gtctggctca cttccgttta gggcacgata atagcgatct cccaccttga taagtgcgcc
     9001 atctgtgttt gtggaagtat taacatagac ttctgacttg tctactccca aatctttgga
     9061 aacaacctcg atgaaatgaa gtgaagtttg aaatttattg ttagaaactt gattgttttg
     9121 ataagtcgtg atgctgacca aaagcagagc taaataaggc cagagctgaa atcatgacca
     9181 actcacgaaa ttcggttccc tttttatcat ggtatgcctt gaaagcaaaa aaactgtcat
     9241 agctaataga acaatcccaa agccaaccat gataccattt tgctgaccga tttggctgag
     9301 tacatagtca taagagtaga atttcatgta ttttcactcc ctttcataaa atcattctta
     9361 attataaacg aaagagagta atttttcaaa ccatacgttg gggaaataga tctttttttg
     9421 gtataataaa atctataatc tgaatgaaaa ggtaacttta tgaaacttat ctcatggaat
     9481 attgattccc taaacgctgc cctaactagt gactcagctc gtgccaaatt gtcccaagaa
     9541 gtcctacaaa ccttggtcgc tgaaaatgct gatatcattg ctatccaaga aaccaagctt
     9601 tctgccaaag gacctacaaa gaaacacgtg gaaattttag aagaactctt cccaggctac
     9661 gaaaacacgt ggcgctcttc ccaagagcct gcccgtaaag gctatgctgg aaccatgttc
     9721 ctttataaga aagaacttac acccactgtc actttcccag aaatcggtgc cccttctacc
     9781 atggacttgg aaggccgcat catcactcta gaatttgatg catttttcgt gacccaagtt
     9841 tacacaccaa atgctggcga tggtcttaaa cgcttggaag aacgccaagt ctgggatgtt
     9901 aaatatgctg agtatttggc tgaactagac aaagaaaaac cagtccttgc gaccggtgac
     9961 tacaacgtag cccacaatga aatcgacctt gcaaatcctg ctagcaaccg ccgttcacct
    10021 ggatttactg acgaggaacg tgctggattt accaacctat tggcaactgg atttactgat
    10081 accttccgcc atgttcatgg cgatgttcct gaacgctaca cttggtgggc acaacgcagc
    10141 aaaacttcta aaatcaacaa tacaggctgg agaatcgact actggctcac aagtaaccgt
    10201 atcgctgaca aggtcactaa atctgatatg attgactcag gtgctcgcca agaccataca
    10261 ccgattgtct tggaaattga cttgtaagga ttttctatat ggactacaat gcggtcattc
    10321 ccgagttgct tgtatcgaat atcgaacagt cccggtcctt ctactgtgat ttactgggtt
    10381 ttaggattga ataccaacgt ccagaagaaa actttctctt tctcttgaag agtgtcaact
    10441 aatgttagag gagggaacaa aggatcaact agctgaactg acctatctat ttggtcgtgg
    10501 agtcaatctc tcctttggca taaaggatgt ttcaaaactc tatcaaagag tgatgaaggc
    10561 aaactatcct atttatcgtc ccttgaccaa aagaaagttt cgtgtcagtg atcactatat
    10621 ctatcctcat gaatttgcag ttttggatcc agatagctat tttttaagat ttagcgagta
    10681 gaataataaa aattgggaaa agtctttaaa gttagaaaaa ggcatattgt caggtgtcta
    10741 aaaacttgat agtatgcctt ttatgatgga aaaatcttct aatactcttc gaaaatctct
    10801 tcaagccacg tcaacgtcgc cttaccgtag gtatatgata ctgactctgt caattctatc
    10861 cacaacctca aaacaatgtt ttgaggttgt ggatagcttc ctagtttgct ctttgatttt
    10921 catttagtat aaatgttagc ttacaatgga gtttttgttc acccctctct tctgtttgtt
    10981 cgttttttaa accaaaatac taacaaaata gttgatttct aaagttattt agtgtaaaat
    11041 aaaaacattt gctcaagcct atttaaaatt gaatatcgtt ttacttgttt atgtcgtgaa
    11101 ttggcacgac gtttctacaa ggtgccggaa cacctaacaa taagtaagtc agcagtgaga
    11161 tatggccgtt tttgccatgt ctatttgggg gacttactta gagattaagt aggtcttttt
    11221 tgaatatttt taatatttcc cttaattagt gcggacggga ggtgaaatta tcctgtgaat
    11281 aatttcagga atccagggaa tttcatccct aatttttgag cccctagctc tttcatttct
    11341 aggcttaggc taaaatagtt ccccgaacta ttttactctc aaaaatcccg tcatagacaa
    11401 taacaagatt attgtctatg ataccactac aacgggaaat atttgataag actcgcttcg
    11461 ctcgtaatag ttttatcaaa tatatagtat tagcaaaagg gatcactata gaaacattta
    11521 aacaacaagt aaattaactt ttagacttta ggaggtctta tgaaattatt agaagaacgc
    11581 atcctcaagg atgggcatat cttgggtgat aacatcctca aggtagattc ctttttaacc
    11641 caccaagttg actttagctt gatgcgagag attggtaagg tttttgcgga aaaatttgct
    11701 gctactggca ttaccaaggt cgtaaccatt gaagcatcgg gtattgcccc agccgttttt
    11761 acagctgaag ccttaaacgt tcccatgatt ttcgccaaaa aagctaagaa catcaccatg
    11821 aacgaaggca tcttaactgc tcaagtctac tcctttacca aacaggtgac cagcaccgtt
    11881 tctatcgctg gaaaattcct ctcaccagag gacaaggttt tgattatcga cgatttcctt
    11941 gctaatggcc aagctgctaa aggcttgatt caaatcatcg aacaggccgg tgccacagtc
    12001 caagctatcg gtatcgtgat tgagaaatcc ttccaagatg gccgtgattt gcttgaaaaa
    12061 gcaggctacc ctgtcctatc acttgctcgt ttggatcgtt ttgaaaatgg tcaggtcgta
    12121 tttaaggagg cagatctcta atgcaaactc aagaaaaaca ctcgcaagca gccgttcttg
    12181 gcttgcagca cttactagcc atgtactcag gatctatcct ggttcccatc atgattgcga
    12241 cagcccttgg ctattcagct gagcagttga cctacctgat ttctacagat atcttcatgt
    12301 gtggggtggc aaccttcctc caactccaac tcaacaaata ctttgggatt ggactcccag
    12361 tcgttcttgg agttgcattc cagtcggtcg ctcccttgat tatgattggg caaagccatg
    12421 gtagtggcgc tatgtttggt gcccttatcg catctgggat ttacgtggtt cttgtttcag
    12481 gcatcttctc aaaagtagcc aatctcttcc catctatcgt aacaggatct gttattacca
    12541 cgattggttt aaccttgatc cctgtcgcta ttggaaatat gggaaataac gttccagagc
    12601 caactggtca aagtctcttg cttgcagcta ttactgttct gattatcctc ttgatcaaca
    12661 tctttaccaa aggatttatc aagtctatct ctattttgat tggtctggtt gttggaactg
    12721 ccattgctgc tactatgggc ttggtggact tctctcctgt tgcggtagct ccacttgtcc
    12781 atgtcccaac tccactctac tttgggatgc caacctttga aatctcatct attgccatga
    12841 tgtgtatcat cgcaacggtg tctatggttg agtcaactgg tgtttatcta gccttgtctg
    12901 atatcacaaa agatccaatc gacagcacgc gccttcgcaa cggttaccgc gcagaaggtt
    12961 tggcggtact tctcggagga atctttaaca ccttccctta caccggattt tcacaaaacg
    13021 ttggtttggt taaattgtca ggcatcaaaa aacgcctgcc aatctactac gcagctggtt
    13081 tcctggttct ccttggactg cttcctaagt ttggtgccct tgcccaaatc attccaagct
    13141 ccgtcctcgg cggtgccatg ctggtgatgt ttggttttgt atctattcaa gggatgcaaa
    13201 tcctcgcccg agttgacttt gctaacaatg aacacaactt ccttatcgca gctgtttcaa
    13261 tcgctgcagg tgtcggtctc aacaacagta atctctttgt cagcatgccg acagccttcc
    13321 aaatgttctt ctcaaacgga atcgtcgtag ccagcctact cgctattgtc ctcaatgccg
    13381 tattaaatca taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tttatcacaa
    13441 attgattata aatacaatca atcccaattc ccatctaatt gaattggacc tatttcaaat
    13501 tc
//
LOCUS       DAVLTM010000050        13049 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLTM010000050 DAVLTM010000000
VERSION     DAVLTM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.051"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(207..1712)
                     /gene="pyk"
                     /locus_tag="VYN16_001232"
     CDS             complement(207..1712)
                     /gene="pyk"
                     /locus_tag="VYN16_001232"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1403762.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(1771..2778)
                     /gene="pfkA"
                     /locus_tag="VYN16_001233"
     CDS             complement(1771..2778)
                     /gene="pfkA"
                     /locus_tag="VYN16_001233"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1403763.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEADFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(2861..5962)
                     /locus_tag="VYN16_001234"
     CDS             complement(2861..5962)
                     /locus_tag="VYN16_001234"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1403764.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVLEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            6310..8583
                     /locus_tag="VYN16_001235"
     CDS             6310..8583
                     /locus_tag="VYN16_001235"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1403765.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELKVKLPHKIELEKPQLNLVQPQGQAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQAFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRSDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            8600..9070
                     /locus_tag="VYN16_001236"
     CDS             8600..9070
                     /locus_tag="VYN16_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1403766.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(9187..9342)
                     /locus_tag="VYN16_001237"
     CDS             complement(9187..9342)
                     /locus_tag="VYN16_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403767.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKATLTRIEEI
                     FEEYKSS"
     gene            complement(9613..12963)
                     /locus_tag="VYN16_001238"
     CDS             complement(9613..12963)
                     /locus_tag="VYN16_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1403768.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         3924 a         2871 c         2222 g         4032 t
ORIGIN      
        1 cgaaactttg aaaacttcaa aaaacggatt tttatcgctt tgaacatcaa aaaagaaagg
       61 acgaaatttg tcctttctcg agcttagctg acttcaaccc actacagttg acaaagagcc
      121 cattatgaaa gttttgcccc ctggacaaaa aaaagagccc atcatacagg ctagaaagct
      181 agatatgata ggtttttata tttttcttaa cgtactgtgc ggatacgcat tgtgtttgtg
      241 cgaacagctt ctcctactgg cacaccagca acgataacga tatcatcgcc tgactcaacg
      301 agacctgctt ctaccgcttt acgttcagcg atttcgaaca tatcgtcagt tgaagatgga
      361 gcatctgtca acattgggat aacaccccag ttcaacatca agccacgttc tgtcaattcg
      421 tcaaatgtca atgctaagat gtcagcattt ggacggtatt tagaaatcaa acgtgcagta
      481 tgacctgtct tagtaagagt tacaaccaat ttgatatcca ttgagctagt agcatcttta
      541 acagcagaag ccattacttc tgtcttagag ttacgctcaa atgaatctga atcaagacgt
      601 ccgtattcat taagaagagc ttgagcgttc ttgtcgattg tagccattgt agttactgac
      661 tcgagtgggt atttaccgtt tgcagactcg cctgacagca ttgtagcgtc agttccgtcg
      721 ataacagcgt tgaatacgtc tgatacttct gaacgagttg cacgtggttt ttcagtcatt
      781 gtttcaagca tgtttgttgc agtgataaca actttacctg cagcattgac tttcttgata
      841 atcatttttt gataaactgg aaccatttcg aacggtactt cgatacccat atcaccacga
      901 gcaatcataa taccatcagc tgcttcgata atttcgtcta agttatcgat accttgttgg
      961 ttttcgattt tagcgaacaa ttgaacatgt ccgtttccag tttcttcaca gattgcacga
     1021 acttcgttca catcttttgc agtacgtacg aatgaaattg cgatgaagtt gataccttgt
     1081 tcaagaccga aacggatatc gtcgttatcg cgttcagcaa gagctgggaa aggaatttta
     1141 gtgttaggga tgttcacacc tttttgttta gcgatgatac catcgttttc aacttcaact
     1201 tcaaattcac gagttgcatc atctttagca accacacgaa gaccaagttt accatcgtca
     1261 accaaaactt gacgaccaac ttcaacatca tcatagatat caagagcacc agcaacgttc
     1321 aacgcaatca cttcacgagt tgatttgatt ccttgtttag ttgcaacacg aattttttca
     1381 ccagttttgt atgaatattc tttagcttca ccttcgaaca attctgtacg gatttcaggt
     1441 ccttttgtat caagaaggaa accaactttt ttacctgcaa ttttttccgc aagtttaaca
     1501 gttgccatac gctcaccttg ttcttggtgg tcgccgtgtg agaagttgaa tcggaatgtg
     1561 ttagcaccag cttcaatcaa tttagcaatg tttttagctg aagcttcaac atcaagtttt
     1621 tcaccccagt atccgtcctc accgaatttt ttaccaccac ggatttctac cgcaggaccc
     1681 aaagttgcaa cgatttttac acgtttgttc atgatttttg tgactccttt atatatatga
     1741 cctttttggt cttattaact aaattgtaaa ttatgacaag ctcttattca agctagatag
     1801 ctcaatatcg gctttgtgtg ggttgttaac cacaatctta ccttctgcag taaggctaaa
     1861 caatgcccct tcttctgcag taccaagaat tggattttca accatttttt cgttacgaat
     1921 accaaccgca acaccaccga taccttcttt aagaagttta acagcatgtg cacccatacg
     1981 tgacgccaaa acacggtcac gcgcagttgg agaaccacca cgttgaatat gtccaagttc
     2041 tgttacacga aggtcgcttg tatctccagc ttctttaagt ttttgaccaa attcagccgc
     2101 tgacatcaca ccttcagcta agacgatgat attgtgtttt ttaccacatt cataaccagc
     2161 tttgatgctt gctacgatat cttccatctt gaagtctgct tcagggatga tgatttcatc
     2221 agcaccagtt gcaataccag cccaaagagc gatatcacca gcgttacgtc ccataacttc
     2281 gattacaaaa gtacgacggt gacttgatga tgtatcacga atcttatcaa tagcgtccat
     2341 ggcagtagta accgctgtgt caaaaccgat tgtaaagtca gtaccaacga tatcgttatc
     2401 gattgtacct ggaagaccaa tagctgggaa gccatgttca gtcaaacgca tagcgccgtg
     2461 gtaagatccg tcaccaccga taacaactac accttcaatt ccgtgttttt tcaattgctc
     2521 aatcccttta agttgccctt caagttgagc gaactctgga taacgagctg agtgaagaaa
     2581 agtaccacca cgagaaatga tgtctcctac tgaagctgca tctaggggat gaatttcacc
     2641 agcaaccata ccagcatatc cgtcatagat accaaacact tccattcctt ctgaaattgc
     2701 ttgacgaaca actgcacgga tagcagcgtt cataccaggt gcgtctccgc cactagtcaa
     2761 aacagcaata cgtttcatat tggtttatgc tcctttttct tttaacattc ttcttgatta
     2821 tatcacattt gattttaaaa ttcttctatt ttccgtattt ttagcgataa atcgttttca
     2881 taacgatttc attcaatttc tcctctaatt cattggattt agctacaaaa tgatggggag
     2941 aaacgatggt tttctgttcc tcttcatacc ggatgatgac tgggattggg cctttaaatt
     3001 gttctaaaat acgtgaaatt tcttgatccg attcatgatt tttcacctgt atccaaaagc
     3061 gttcagcaac tgcttctctt atttcttgtg caatcatttg cagacggcca tcacgtgatt
     3121 gtatttttcc ttttacatag tagaaggctc cctcttttat ttcctgtccg acctgacgat
     3181 ataagtctga aaagagagtg acatccaatt ttttcttact atcatctgcc tgtaagaagg
     3241 ccatattttc accctttttg gtacgaatca cttttatttt ctgaacttca accaaaataa
     3301 tagcatagct attttctgac aaatttccga ttggggtaat cgggtaaata gccttacttg
     3361 caatagcttg tagtggatgt ttgctgacac ctatccctaa aagctcttgt tccatataaa
     3421 atttttcttg ttccgtccaa tcttccgatt cctgccaact ataaatagca tctccaaaca
     3481 aacttcccaa ctctttcaca aattcaaata gattagctaa gttattaaat actttttgac
     3541 gatttttttc aaatgaatcg aaaagaccaa cttttaccaa aggttctagc agaggaagtt
     3601 tcagataatt ctcaggtaat ttagctataa aatcttcaat gttagaataa ggtctatgtt
     3661 caataatcca gagagctaaa tcattactga ctcctttaat ggatttcaaa cctagataga
     3721 tggccttgtt ggcaatttta tcgtgatagg gaatcgtgtt gatggacaat ggggctactt
     3781 caaaacctgc ttctaaggca tctgttacat aatcactgtt agcagaattc aacataatct
     3841 gataaaatat ggctggataa tgtgttttaa aataagccaa ctggaaggcc aaggctgagt
     3901 aggcgtaggc gtgtgacctg ttaaaaccat agcctgcaaa cttctccaga acatcaaaaa
     3961 cctgctctgc tttttccgca gtatggcctg cttctatgga gccttgaata aaggaagccc
     4021 tcatctcatg catagcagaa gcatcctttt tacccatagc tcgacgcaaa atatcggcct
     4081 tcccaagact aaatccagca aatcgctggg caacctgcat aacctgctcc tgatagagca
     4141 ttatgccgta ggttggagcc aaaatatcct ccagtactgg atccagaaca gtcacttctt
     4201 cctgcccatg ctttcttgcc acaaaattat tgatatagtc actagcaccc ggtcgattta
     4261 gagaagtagt cgcgacgaca tcttcaaaac agactggttg cacacgctta agcagacgaa
     4321 tggcacctgg ttgctcaaat tgaaagatac cttttgtatt accagaggca aataaagcta
     4381 acgtttcttt gtcttctaaa tcgatttctt caattttcag atgaatacct tctgtttcag
     4441 caagcaactc ttgcatcttc tggacaaagg tcaaatttcg tagtcccaga aagtccatct
     4501 tcaaaagtcc gctagcctca actccatgag catcatactg agtcagtgga atttcatcac
     4561 catactttag aggaatgtag ttggttaaat cttggtcact aattacaaca ccagccgcat
     4621 ggacagaggt ttgccttgga tagccctcta tcttgcaagc aatctcaaaa gctttttggt
     4681 attctaactt actattgatt tgctgacgaa actggagatt gccctcatag gccgacttaa
     4741 gattgtcacg aaaactgatt ttcttagtaa ttgcagataa ttcatactct ggcacaccaa
     4801 agcgtttcaa gacatctcga agagcttgct tggctccaaa ggttgaaaaa gtaacgattt
     4861 gtgccgcatg tttactacca tatttattac caacatatct gataaaatct ggacgataaa
     4921 tatctgggat atcaatatca atatcaggca tggtatagcg ttcacgatta agaaagcgtt
     4981 caaaaatcag atttttctct actgggtcaa tccccgtgat gtctaaggca taagaaacca
     5041 aactgcctac tgcagaaccc cttcccattc ccatataata gccattcgat cgtccaaaac
     5101 gcaacaaatc ccaaacaacc aagaaataat catcaaagcc catatcatga ataacagaca
     5161 attcttggtc tagtctatct tgatattctt tactagtcaa ccccttctga acaagcccca
     5221 gttcagcacg ctctctcaac tcctctactg ctggtctagc tggattaaaa cgaggcagtt
     5281 tcagactagt atccaagtcg taagaaatgc ctgaaataag cttttctaaa ttgtccaaag
     5341 cttgcggaaa acgctcttgg aatagtttct ctaaagaact tgctgatata aagacatctt
     5401 gtctcgaacg caagggaact tctctgagcg gtagattttc tttaatcgct gttaaaactt
     5461 gaagaacttc tctatccctg ctttcaaaag cgttgacccg ataaagaggt aagataggat
     5521 gatgaaattc gcttgctagt gtttctggat aaacccctat atagtaatca cagcctagtt
     5581 ctaacgactc aactctatca aaataaggca caatgaccgc gatatcctcc aggtactggg
     5641 acaggactga ccaagttttc tccccctgca tcttggctgt cgaaagcttc atcaactgct
     5701 gatagcccac actagataga gctaaaaagc gcaaattcac ttcctgatca tctacaaaca
     5761 ctgtcatttc aagccctagc aaaggatgaa tgccgtattt ttttgtaatc tctagaaagt
     5821 cgaaagcgcc ataaagattg tcaatatcca tcatagccaa atgagtgtag ccgtattctt
     5881 tagctgctct cacatacttt tcgatcgaaa tgacgctttc cataaaacta tagactgttt
     5941 ttgtatctag ttgtgcgatc aatttacact tctcctctat ccttctcact atattatacc
     6001 attttcacct ataaatggct tctcttgaga aaaatttcat cagcttacta atttattttc
     6061 tattcatttg tatttttgtc atcattttta tatttatact atattgtata taaaaataga
     6121 aagcatataa tataaatctc ctagaacttt tataccctat ctattttatt cttattgtta
     6181 ctctgttctc tatattgaat ttagtacttt attcattttt attggattat ttccttttga
     6241 agttaacaat tccctttctg cttttttttt gatataataa ccttaactgt cattctatat
     6301 ggaggttcta tgcgttttaa tcaatatagt tatatcaatt ttccaaaaga aaatgtctta
     6361 tctgaactaa aaaaatgtgg ctttgattta caaaatacag ccaatcacaa agactcactt
     6421 gaaacctttt tacgtcgttt ctttttcact tatcaagaca ccaactaccc actttctatc
     6481 ctagcagctg ataaaaaaac agacctgctg acattttttc aatcagaaga tgaactgaca
     6541 gcagatattt tttatactgt tgcttttcaa cttttaggct tttcttattt ggttgacttc
     6601 gaagatagtg acgtttttcg taaagaaact ggatttccca ttatatatgg tgacttgatt
     6661 gaaaatctct atcagttact caatactcgc accaaaaaag gaaatactct tatcgaccaa
     6721 cttgttagtg atggtcttat tccggaggat aatgactacc actactttaa cggcaagagt
     6781 ttggctactt tttctaacca ggatgtcatt cgtgaagtcg tttacgttga gtctcgtgtc
     6841 gatactgacc aaaaagggct atcagaccta gtaaaggtta gcattattcg ccctcgtttt
     6901 gatggaaaaa tccctgccat catgacagcc agcccttatc atcagggaac caatgacaag
     6961 gctagtgaca aggctctcta caagatggag ggcgagctta aggttaagct tcctcacaag
     7021 attgagctag agaaacctca actaaatctc gtccaacccc aaggtcaagc tgagcttata
     7081 gcagaggctg aggaaaagct aactcacatc aactctagct atacactaaa cgactacttc
     7141 cttccgagag gctttgccaa tctctatgtc tcaggtgttg gtaccaaaga ctcgacaggt
     7201 ttcatgacta atggagacta ccagcaaatc gaggcttata aaaatgtcat cgattggctt
     7261 aacggccgtt gccgtgcctt tactgatcac acgcgccagc gtcaagtcaa ggctgattgg
     7321 tcaaacggaa aagttgctac aacgggactt tcctatctag gtaccatgtc caatggtctt
     7381 gcgactacag gagtcgatgg tttagaagtt atcattgccg aggcaggaat ttcatcatgg
     7441 tacaactact accgtgaaaa cggtctggtg actagcccag gtggttatcc aggtgaggac
     7501 tttgactccc ttgctgagtt aacctattct cgtaatctct tagctggcga ctatatccgt
     7561 ggcaatgaag ctcaccaagc tgacttagaa aaagtgaaag ctcagctaga ccgaaaaact
     7621 ggggactaca atcagttttg gcatgaccgc aattatctgc tcaatgccca taaagtaaag
     7681 gcagaggttg tctttactca tggttctcag gattggaatg tcaaaccact tcatgtttac
     7741 cagatgttcc atgctcttcc tactcatata cacaagcatc tctttttcca taatggtgcc
     7801 catgtttaca tgaacaattg gcaatcaatt gacttccgtg agtccataaa tgccttatta
     7861 accaagaaat tactaggaca ggaaacagac tttcaacttc ctactgttat ctggcaggac
     7921 aatacagctc cacagacttg gttatcactt gataacttcg gtgggcaaga aaactgtaaa
     7981 accttctcac ttggtcaaga agagcaagct attcaaaacc agtacccaga taaggatttt
     8041 gagcgctatg gtaagacata ccaagccttc aatacagagc tctatcaagg gaaagccaat
     8101 cagattacta ttaaccttcc tgtgactaaa gatctccacc taaacggtcg cgctcagctc
     8161 aatcttcgta tcaaatccag tacaaacaag gggctcttat ctgcccaact gctagaattt
     8221 gggcaaaaga aatacctaca accttatcca gctattttaa gtgctagaac cattgacaac
     8281 ggtcgctatc acatgttgga aaatctctgt gaattgccat ttagaccaga ggcacaacga
     8341 gtcgtgacaa aaggttacct taatttacaa aatagaagtg atttactgtt agtagaggat
     8401 attactgcag atgaatggat ggacgttcaa tttgaactgc agccaactat ttacaagcta
     8461 aaagaaggag acactctccg tttagtcctc tatactactg actttgaaat caccatccgt
     8521 gacaatacag actaccactt gactgtcgac ctcgctcagt ccatgcttac cttaccttgc
     8581 taaaaggagt tacattatta tgaataaatc agaacaccgc caccaactta tacgcgctct
     8641 tatcacaaaa aacaagattc atacacaagc tgagttgcaa gcccttcttg ctgagaacga
     8701 cattcaagta acccaggcaa ccctctcacg cgacatcaaa aatatgaacc tatcaaaagt
     8761 ccgcgaagaa gatagcgctt attatgttct taacaatggt tccatctcaa aatgggaaaa
     8821 acgtctcgaa ctctacatgg aagacgccct tgtctggatg cgcccagttc aacaccaagt
     8881 cctactaaaa acccttcctg gactggctca atcctttggt tctatcattg atactttgag
     8941 cttccctgac gctatcgcta ccctttgtgg taatgatgtc tgtcttatca tctgtgaaga
     9001 tgcagatact gctcaaaagt gctttgaaga actgaaaaaa ttcgccccac catttttctt
     9061 tgaagaataa taaatgacta caactaacaa ttatgcttaa aaaaaaactg gtagaaagtc
     9121 tttaacatca gaaaacgcat agtatcaagt gttgaaaacc cttgatacta tgcgttttgc
     9181 tttatcctag gaagatttat actcttcgaa aatctcttca atccgcgtca acgtcgcctt
     9241 gccgtaggta tggttactga cttcgtcagt ttcatctaca acctcaaaac agtgttttga
     9301 gcagcctgca gctagtttcc tagtttgctc tttgattttc attgagtatt atttcatttt
     9361 ctcctgcaat tgaattcttg ctcagctttt tgttttctat ttctttaaaa tcaaagtagc
     9421 tcttttgtta ataactcgat caacaaacat cgtggtacaa gtatctactt tgaaatttat
     9481 caaccactta acaactgata ctgtattcta ggaaaacgat gacattcttc ctaataaaac
     9541 ttctcatata tagcataaat ttctactctt tttaatttta aatgagtctc agcatggcaa
     9601 tcactttaga atttaagccg cttctccagc tcgattattg ataagttcaa tgatttgaac
     9661 aatattacga agtactggta gttggtgttg gaagagtagt tgaacagatc cataagattt
     9721 aaacggctct tgcgttaaca ctttcgtctc taaaaaacca ttttctacaa tgtagtctac
     9781 aatcaatttt acaaatgaaa tctgcctggc attaagattc tcatccgaca aaaatttaga
     9841 aaaaatacga ttggcagact ctctatctaa accaataatc tcacgaacca atctcggaat
     9901 tgccttattt tcataatgac tttggtagtc tgctttacta cctaattttt cccaaagcaa
     9961 tttttcaagt gcaagcatat catcagatgt caacttttta ttatggtata gcttagaaat
    10021 ggactcctca tccagatgag ttttcaaata atgctcaact ttttcattgt aagaccgaag
    10081 atcgttgacc tgcaaaaatg ctgtggtctc gtgaacagtg gagagtatac gatcttcaaa
    10141 gttaacatag tagggtttac ggtctgtttt atccaaaaac tgtaataaat ctcgaatagc
    10201 aagacgaatt ttttccaaat cagacaagtt aacttctttc caaaattcag gctcctgtac
    10261 tttcctgata atttcagcct gctcaaaaac ctgcgggata ttgccaatag cagaaagagc
    10321 tctagccgtc ttcatcactt gggaaatatg aacagtggaa gatttagctg tcagttgccc
    10381 caactgaata tgaagcaacc acaaatcaaa tctcctcgcc atctcatctt ctttatcttc
    10441 atcaaataaa agcggagaga gatttttttg aatggtttca cttgtaacag cagttagatt
    10501 ctgccaactt tccaatttcc tatagctata aactgtatct aaaaccatac gaaccctgaa
    10561 gtccaactca tttaagctct ctatacgacc ttgaagttcc gagacaagct gctgacggta
    10621 tgctctcgca aactgatctt cttggtattg gagtccctga agttctcgaa tcaagtccac
    10681 tttgatatta aataaacgct gagtcagcga aacaatgtga cgaccctctc catctcttgg
    10741 atctgcacga aaataatcaa aattgtcccc ataatcaaat accaagaagt tttccttatc
    10801 ctgctcaggt ccaaataaat ctttacatag acgggttcct cgaccaatca tctgccaaaa
    10861 cttagtttta gagcgtactt tcttgaagaa gactaaatta acaacctctg gtacatcaat
    10921 acctgtatct aacatatcga cagaaatcgc aatttgagga tacttctcct taattttaaa
    10981 atcatcaatc aaggtctgat aatgcttaat actataatca atcacctgag catagtcgct
    11041 ccctttttca ggatagcggt tgttaaaaat acctctgata tattccgcat ggtcatgatt
    11101 tttagcaaaa ataatagttt taccaatttc atcacccgag gctgtctgaa ttcctcttgt
    11161 catgagttca tttaaaacaa tttctactgt acttttattg aaaataaagg aattaaacac
    11221 actcccatca atatcttttt cacagctatc gtcttcaaat ttgctatcaa aatgttcctt
    11281 ttcttcttcg gacaaatcat cataatgtag accatccgta ggtagtttca gtttggtttc
    11341 gatagaatga taggctacta aatatccgtc tttaacagcc tcttccaaat catatgcata
    11401 tgttggaacc ccattctcca aattaaagaa tccataggtg tttttatcta aatcttgacg
    11461 cggagtagct gttaagccta caattcttgc atcaaaataa tcaaaaatgg acttgtattt
    11521 ctgataaata gaacggtgag attcgtcaat tatgataagg tcaaaatgcc caacagtgaa
    11581 agggcgttga tttacttctt cttgaccact aatcgctcca atcatggtcg gataagttga
    11641 aaagaccatg cgacttgatt gagctccttc tttatcttct aagaagttac aaacggaaag
    11701 atctgggagt aattttctaa acgaatcata ggcttgctta accaaggaag ttctatcggc
    11761 taagaagaga acgtttttta cccagttatg acgtgataag atatcaacta gagaaactgc
    11821 tgtacgagtt ttcccagccc cagttgccat aaccaaaagt gcctgtctac gatgattaga
    11881 gaaagcttca caaacgcttg caatggcgtg tttttggtag tgacgcccgg aaatatcgtc
    11941 cctaatttta ctagaaatat cctcaagtgg tttttgaaga tgacgtctat ccatcactaa
    12001 ttgcaattct tcttgagagt aaaagcctgc aatctggcgg cggttcggac catcaagtat
    12061 atagtgcttc aagccatttg taataaagca aattggctga tagccgatat gtttctcaag
    12121 agcttcagca tattctttga cctgtacttc ccctacttct ggattgacag aggctttttt
    12181 agcctctaca atcgctaaaa ttttcccatt tttaccataa agtacatagt cacagtaacc
    12241 aattcctgaa ccgtgcttga gaccatcaac ggctatctca acacgacagt tttcttcaaa
    12301 tatccatcct gctaaacgga gatcgatatc aataaagagc tttcttgtct ctgcctcaga
    12361 taattgatca atatggaaag gcacatcttg ccgttgcgct gcagtctcac gtttttcagt
    12421 ataagtctct tgaacttcta cggactgagc agccatctgt tcatgaaaat caggcagttc
    12481 tggtaaactg tcttgtaact ttatcataga ttgtggagtt tctcggtatt tgatgtttgc
    12541 tgaaagtggt aagcacttct catcaaaata acgctccaca aactcattgc tgtaacaata
    12601 atcgataaaa ttaacaaact gatacaaatg atgcaaagct aaaatcgctt ctcgttcctt
    12661 aatttcacca ccatgagcag catggtttcc ccaccgaatc aacagaacta tctgcttgtg
    12721 gagttcagaa tctacgatat ccctaaaatc atcatcccat actaaagaag atagagtagc
    12781 acgataggga gcttctaaat atgaatcgtg actatatatc caatggacag cctgctctaa
    12841 agcacgacgc gccataaagg cagtagccac agttgatgta gcaatcatat tctcagcttc
    12901 aatgcaaggt tttgaaaaag attcatattc gtcatttttt aaaagaaagc taaaatttcc
    12961 catttttttc tcactttaaa caaatagttt agctatatta tactaaattt caagctattt
    13021 ctccacactt ttatagctat ctcacaagc
//
LOCUS       DAVLTM010000051        12718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLTM010000051 DAVLTM010000000
VERSION     DAVLTM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.052"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..618
                     /locus_tag="VYN16_001239"
                     /pseudo
     CDS             1..618
                     /locus_tag="VYN16_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(670..1032)
                     /locus_tag="VYN16_001240"
     CDS             complement(670..1032)
                     /locus_tag="VYN16_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403769.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1323..3203)
                     /locus_tag="VYN16_001241"
     CDS             complement(1323..3203)
                     /locus_tag="VYN16_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1403770.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3197..4066)
                     /locus_tag="VYN16_001242"
     CDS             complement(3197..4066)
                     /locus_tag="VYN16_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1403771.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIRGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4149..6794)
                     /locus_tag="VYN16_001243"
     CDS             complement(4149..6794)
                     /locus_tag="VYN16_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1403772.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6885..8165)
                     /locus_tag="VYN16_001244"
     CDS             complement(6885..8165)
                     /locus_tag="VYN16_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1403773.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8336..10420
                     /locus_tag="VYN16_001245"
     CDS             8336..10420
                     /locus_tag="VYN16_001245"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1403774.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDVAPDLEGKLFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10460..12139)
                     /locus_tag="VYN16_001246"
     CDS             complement(10460..12139)
                     /locus_tag="VYN16_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1403775.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIHWIGNERGH
                     AGDPLWQKMNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLCKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3496 a         3033 c         2592 g         3597 t
ORIGIN      
        1 gagaataccg cacttatgaa gaaattgcgg ctgattttgg tatccacgaa agcaacttaa
       61 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa
      121 ctcctctcag ctctgaggac acggtaatga ttgatgcgac ggaagtacaa atcaatcgcc
      181 ctaaaaaaac aattagcgaa tcattctggt aaaaagaaat ttcacgctat gaaggctcaa
      241 gcgattgtca caagtcaagg gagaattgtt tctttggata tcactgtgaa ctattgtcat
      301 gatatgaagt tgttcaaaat gagttgcaga aatatcagac aagttggtaa aatcttggct
      361 gacagtggtt atcaagggct catgaagata tgtcctcaag cacaaacttc acgtaaatcc
      421 agcaaactca agccactaac agctgaagat aaagcctata accatgcgct atccaaggag
      481 agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc
      541 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat
      601 catgaactag gattctagtt ttgcaggaag tctattgaaa tactaagcct acacagactg
      661 atttcaattt taccattctt catagtcaaa accatcataa tctggatatt gttctggcga
      721 cgttccaacc gaacgtacga gataagcttc ttcgtcctct gtctcgattt ctctcagcac
      781 ttggcattca aaacgccatt catcaccgaa gtcgaaaata aacttaaatt tttgtttgac
      841 acttaggcta tccagataga cattttctgt gtaacgttct tcaacatcgt cactaacaaa
      901 gctaagaaag taagaatctg catgactcca ctcaatatta tccatgaaaa atgcgtgagc
      961 atgatcattt gcaaaatcaa aactccataa aatcacatct gctagttccg ctaacgtttt
     1021 tgtttttgga atctatatat gtcggtagca acctttttta aatttgacgg aaatgatcca
     1081 agcctctaca gatgctggct tttcagcttc tatttcttgt tttactaatt ttaactgtag
     1141 catttgaaac cagataattc ttctgtttca cgcgaaacac cctgcgccaa gcaaggtgtt
     1201 cttgttttta tactcaatga aaatcaaaga gcaaactagg aagctagctg caggctgctc
     1261 aaaacactgt tttgaggttg tagatggaag ctgacgtggt ttgaagagat tttcgaagag
     1321 tattaagtcg tataaatcgt cgaagctgtc gcaatggtat gccactggtt agctgtagtt
     1381 gctgcgaaat ccttgtctgc gtagaagtca gtaaatggta ggatttcaac ttccagctcg
     1441 tcgatgcggt caagctggcc agccagataa acctcgatac ggctttctgc tcgtttgatg
     1501 cgttgcaaga gtccgcccat acggatgtca actgtatcca aaccaaagac cttgttttct
     1561 ttcagccatt ggtgactaaa gagggcatgg aagtcttcaa tttggcttct aagttctggt
     1621 aattcttgtc tggcgatttg ttgtaaactt tctttatcat ccgcttggta ggcctgacga
     1681 atgcgtcgtc ccacatctac tttgctactt aaaatagcat tcaactgggc ctgagtttca
     1741 aagagataag catagtttcc agctttttct ttaatgttag caagcgtctc agcagcctga
     1801 gcgaagtgcg gtttgtcctg ttcaggtgtc atgtgtcggt caagtatcgg acaaagaaca
     1861 tcctgataaa agacatatcg gttgggattg atgccactga gattgcctgg taggtctggt
     1921 aagaggttgg caaggtcaat ctgcataaaa tcctcaaccg ttagaccagt attggtcttg
     1981 aagtgcgcag acaaactatc taggtcatgg cgatagctga gttctgccca gatttgcaag
     2041 cttggtagga tagagaactg ggcagtttca cccccattgt ctccccaacc cgttacgatg
     2101 acttctttaa tctgattggc acggcaggct ttattagcct cgatagccac tagacggcta
     2161 aaatggttgt gaggtgtaaa gccaatccac ttccaagctc cccctgcaaa tgcaaggtca
     2221 tggctaatct tgtgatgatt gcggaaatta cggttgtatt tttcctcgct atcctgataa
     2281 taatcccagt aaaccagagt cacacggtct ttgagacggt ctaggtagac acgagtttcc
     2341 tctggaattt ccacatcacg gtcgtactgg ccatccgctg acatgagttt gaagaacata
     2401 tcactccaca tctggcagtg gaaaccatat ttgtcagcaa tatccagcac gcgctccaag
     2461 tgttggcaca tgaggagact acgatccaca acaccgttca aaatcaggta gcgtcccaaa
     2521 ccaaccaagt gggcttcgtc catcccgata ttgaccttgc gagtcttcag tttagacaac
     2581 gtggcaaaca tgccatcaat caggtcataa accttttctt cgccaatgag aagaatgtcc
     2641 tctacatcac ggagctcctg cacttccttg acaccccatt tgacaaaggc cgacaagtgg
     2701 gccaaggtct ggatgcatgg tacaaaggtc atgtcaaact gttgagcata ggcttcgatt
     2761 tcctgcaact cctctgctga ataagctcca cggaaatagc caaagtaagg ctgtccttca
     2821 atctggtaag tgtcttccat gtaaagctca aaggttgagt agcccatgag agccaagacc
     2881 tcaatcatct gcttggcaga agccacattc agcaccgcat ttcgagaaca gtcaaccatg
     2941 taagccaaat cttcgtaagc tgcttgttcc tcaatctcta ctttatcagc ttctgctaga
     3001 actgttacca acaaggataa ggcacgataa agttggtgag gtttgcggta ggtcaattga
     3061 tagtgacctt cctcaccctg gatagagata gaggcttggt cagactgagt gacagccact
     3121 tccacatctg gtagagaaat gtgacctttt aaaacctcaa tagcttgcgt ttgtttggga
     3181 ctaagtcctg taaatcttac cattgctttt cctcctgtag ccagttgaca agggcaccgt
     3241 agagatttgc atctgcgtga taggtgcagg cctggataac tggggcgacc gtgtattctt
     3301 cgtaggtatc gacaaagtta tcaacagcct tcttgacacc ttggataaaa tctggatttt
     3361 gactgataga gccacccaga ctgatgacat ctggatcgat cagatactgg atattgagca
     3421 agccttgcgc cagattgcag ttcatgcgct caatggcttc ttgacaaaga gcattaccag
     3481 ctgcggcctc ttggtaaatc ttgcgaccgt cccaatcagt atgaccagat ttttcaatta
     3541 cgtatcgtac catattccca gttgatgcta gttgcgacca gttattaagt tttccagcag
     3601 gggcaagggt tgtcatgtag ccaaattctc cacccagacc gtggcgacct cgatgaagtc
     3661 taccattgat aatcatggct ccgccaatcc ctgtcccaat cacgacacag gctgcatttt
     3721 caagctctgg atgagctagt agttcactga gtccaacgca gttggcatca ttttctaaat
     3781 ggacaggtag ctgataagag ctaagagcct cataccaaga aaagccacgg atgtagggca
     3841 ccgcactgaa gccatcaatc acacctgtct cttgattgac tgcacctgga acgctcatag
     3901 caatcccact gtaatcctgt tctgacaagc gttgatctag ccacgctagt aaatcctcca
     3961 agttttcagg cgttgaaata cttgtcttat ccagtatttt cccatcagga gtcagactgg
     4021 caaacttaat cccagtccct ccgatatcaa tcgttgcaat ggtcattgtt tttaccataa
     4081 aagaggcgaa tcagcagtta gttcttactt tttcgcccct cctttctttt gatgtttact
     4141 ttttgaaatt aaatttcttc ttttttgatg aattctgtac gaatctcttg tggagccaat
     4201 agtcctgaat gaactggata tggtcgctca agtaagtcaa gaatggtttg ttgatgttct
     4261 gatatgcgca cattttcttg actcatatta tagtaacgaa ggacatagcc ttcttcattt
     4321 tccgccactt taaaggctgt tggacaaact tgcggtatgc tgagaacaga atggctcaag
     4381 aggctaccag tcgcaaccac gcttccttcc tgtctagcaa gctgaaggct ggtaaacggt
     4441 gtctgcaagg ctttggcacg acgataggct gagaagcgtt cttgggcttg gtggcattca
     4501 agtgcaaact cgacttcaaa ctcacgcaag cattgtgctt ctggcgttgg gaagtagccc
     4561 cagtcaccta gctcacctga tgcacgcaaa atggtcacgg caatggtgtt atccccaagg
     4621 atttcgtatt cattcaatcc cttgttggat acagtcaccc ctttttcatc gtcatacaga
     4681 ctgacaaaag cttgttggtg ttgaggattt tcaggatttt cccatgaagc agctggtttg
     4741 tttggtcgtg tcaccacctc atagatactt tcagaatcat tgcttggacg cgtgttatga
     4801 gtcttgacca agagacggat acggtgatcc ttggcagtgt tagtaaagcg agtcttgaag
     4861 cggatttgtg gattgtcaac gaagacagtc aactcagttt ccagaggaat gtttgtcaat
     4921 tcttctgacc gtccagcctc acgcttcata aactcgatga taccttgttg ctcttcttct
     4981 agcttttcat ccgcactgac aggcacggtc aattcatgtt tgagcaagat tttagcatag
     5041 cgatctgtgt tttccaatac ctcgtggccc ttaagctctg caaagattgg ctctgttcct
     5101 tttggttgga aatagatata ctcgtttccg atgtcacccc ggtcttcaaa gcggataaag
     5161 tcttcatagg cttcgtgagt tgtcttgtca tagactgtga tgttgtcatc cacactcacc
     5221 gttacgaatg gtgtatcaat cactccgttt tggtaaatac cctcacggtg ttcttgtttt
     5281 ccttccagca attggaaggt tgtccaagaa agcggtgcta ggtgaactgg aatggtcaca
     5341 cgcacttgtc gagcaatacg agcttggcgg aacttatctt ttggtaaatc gtattcaaaa
     5401 ttagctccga ggtcttcgat tgtagcctct acaggacgac catccaagtc ctccacacgg
     5461 taacttggca agataagagc agccatcttt ttgtagcctt ctgttgggtg caattccttg
     5521 aaatcacaag tcgccacatc aatcactgtg ctgacagtat cgaccttatc atgcaagcct
     5581 gtgttaatga cagtaaagag atagtcactt tgagccttat ccgtagcaat tttacccttc
     5641 cactcattga gcaaattact tttaacaaag tttcctactt ggttgacctt ggcaaaacgc
     5701 gtttccatct cgcggtgaac ttcgtccacg ctacagccac agatactatc atgtggcgca
     5761 ttctgcaaaa gtgttttcca agcataggtc aactggtcct tgtggttgtg tccaccagtg
     5821 ataatagtca agggttctac cacttgctct aggaggttgc tattttcttg gaaggcttgt
     5881 tttaggtaaa tgcgggatga agaagtgttg gcaagtgtgt accagccatc tgtttcctga
     5941 ctggtcaact cacctgtaac agttgataag tgttcaggaa gcgcaccttc tacagcttga
     6001 acatattcat caaaagaact atgaacaaag attacatccg ggaagagttc atttgccaca
     6061 cgaatggctt cgctcagatt tttctggaca ggctggtggt cacagccgtt catcatcaac
     6121 cattggttgg tcgaagcgta ggcacgcaca tctgacaatt tttgtttcca gaaggtcaag
     6181 gcctcatctt tgtcaactgg gatttcattc ccattactgt accagttggc aaagagaata
     6241 cctaaaacac gactaccatc cacaccctgc cagtacattt ctgaaaactg ggatgtaaac
     6301 tgctcatctt caaggacttg gttgtcaaat ccaatcggct tcacaccacg accaaaggcc
     6361 gccacgtgaa tgcctgattt ttgaagaatt tgaggcgctt gtcccatatt tccaaaggta
     6421 tctggaaagt agccaatctg ggttgattta ccccatttgg cagcttcttg ttgaccaatc
     6481 aaggtattgc ggacattggc ttcactggag atcaagtagt catcctgcaa gatgtaaaag
     6541 ggaccaattt taagtttgcc ctcgtcaatg tagcgttgga ccttgtcacg attttcaggg
     6601 cgaatttgta agtagtcgtc aaggacaata gtttgtccat ccaagtggaa actcttgaac
     6661 tcagggtcat tttcaaagag atcaaagaga ttgtcaaaca attccaccaa ctgcatacga
     6721 tggctttcaa aaggcaagta ccactcacgg tcccagtgac tatgtgagat aatatgtaca
     6781 acaacatttt ccatgaagta aaacctcatt ctaaatttaa attttaacgt tttaaatgta
     6841 aacttgtgat tctgataaga atcggttgag ctaaagcgag ccccttagcg aatatccaag
     6901 taatccaaga ccaactcaca gaacatcatg ttagcccagg agaaccattc acgagagtag
     6961 agggtcggat catctacgtg gaagctttcg tgcatgacac ctgtaccacc atcgcaggca
     7021 accagctgat ccagcaagaa ttttttctct gccttatctc ttgttgtcaa tccttggata
     7081 gaaagggcga ttggccagat atagcgatag aaggtatgag aacttccgag accgctagcg
     7141 tattctcctt ggtagaagta tggattttca gagctcaaaa tggtacgacg agtagcttga
     7201 tacacttcat catcgaccga acagtagccc agatagggcg cagctagtag acttggtaca
     7261 tttggatcat ccatgatgct ggcatttcct aggccatcca cttcaaaagc gtaaatcttt
     7321 tcgcccttgc tgttggtggt gtaagcgtag ttttcgattc cttcttggat ttcatcctga
     7381 agacgcttgg tatcagcaat aacactctgg ctatcagcta ggtttaatgc tgcgaagatt
     7441 tcttgcacat aacccaagac tactacagca aacatatttg acggaatcaa gtaactatac
     7501 tggcaacagt catcactcgg acgaaaggct gaccaagtca tacctgtcac tgcaaagtca
     7561 ggtccaaagc catcatttac caaggtgtct tccttacggt ccgtatctcg gacaaaacga
     7621 taaggagagt tcttgtggtc ttgttcaact gtccatagat ggagaatttc cttagtcgct
     7681 gcgacaaaag tctcatcaaa ctgaccagtc tcgccagtct ctttccagag gagataagcc
     7741 aactgcaaag gatagcaaag cgagtccacc tcatacttgc gttcccaaat ccagccatta
     7801 aggtcggtgt ggtcggtctc gtggtgcccc ttccagtttt cctctatgtt gaaggaattg
     7861 gcataaggat ccttgagtac caaggtcatc tgacgtttga ccaaacctgc aatggtctga
     7921 cgcaggaggg catctctttt agctacatga aggtagggtc tgagttgggc tgtcgaatcc
     7981 cgaagccaca tagcaggaat atccccagtc aagacaaaag ttgaaccatc ttctaagatt
     8041 tcaaccgtat tgtccaaggt atcggtgtag caacgctcga aaacatccac ccactctgga
     8101 tggtccttag cccgctctgc tacttcatct agccattctc taacaatttc tttcgaataa
     8161 atcatcgtgc aatttcctct ttcaaacaag tttcattttc agtatatagc ttttgagaca
     8221 gaaaatacat acacaaatga tagtcatttt tctacaaaat agttattttt gaaactcctt
     8281 ttctaaaggc cctctttttc tgatataatg tagaaaaaca gctaaaggaa agactatgaa
     8341 accactactt gaaaccatcg atacccgctt tggtactgct agcaagcatg ccttctctcg
     8401 gggaaatacc ctgccataca caggcgtgcc ttttggaatg aattactttg tgccccagac
     8461 cagtgaccag gatggagctt ggttttttga tccgcatctg cctatctttc aggggattcg
     8521 attaactcac cagcccagtc cttggattgg ggactactct tggctccttc tgacacccgt
     8581 caccagtcaa ctgggtggag acagcctctt tcaccgccaa tcttcctatg atagggataa
     8641 ggcctctttc caacctcatt atctaaagat tttctccctg cgctatcaga ttgaaaccca
     8701 gctcacaccg acttgctacg gtgcttctat tcgtttgaag caaaagcaag gcaaagccct
     8761 ctccctctat cttcacgcaa cagatgaact gacagtagag caagtagata agcgaactct
     8821 tgccctgcga caagaaggta aaactgaaac caacaaaaat tcgctaacga tgtttactgc
     8881 cctgcaaatg aacacggata ttcttgctat cagccaagaa gctggggact ggcgaattga
     8941 cttagcaagt agtcaaaccg agatgcaact agccacttct ttcatctctc cttctcaagc
     9001 tctaatcaat ctacctcaag aagattttga tagctgtaaa acaagtgccc aagcggattg
     9061 ggaaaatctc ctccatcgtt ttgacattat agagacagga gaggctgacc gaaccttctt
     9121 tgaccactgc ctctacagac tcttcctctt cccacagact ttttatgagg ttgatgagtc
     9181 agggcaagcc atccacatgg atctggctac tggtactgtc aagcctggtg tcctctttag
     9241 caacaatggt ttctgggata ccttccgcac caccttcccc ctctttgccc ttatcatacc
     9301 ggagcactat caacgctttt tagaaggttt cctcaatagc taccgcgata ctggtttcct
     9361 tccaaaatgg ctggctccag atgaacgggg tatgatgcca ggtacacttt tagacggtat
     9421 tatcgcagat agcgcctgca aggacgtggc ccctgaccta gaaggaaaac tcttccaagc
     9481 catgctcgaa acagccagca aagccgaccc tctcggaatc aatggccgcc acggactagc
     9541 ccaataccaa gaactaggtt acctctctac cgaccaccac gaaagtgtta gccatactct
     9601 agactatgcc tatagtgact tttgtatcgc tagctgtgcc aaaaaactag agaacataga
     9661 aatcgctgaa acctacaagg ctgcttcaca aaattaccgc cagctatttg acgctgagac
     9721 aggttacatg cgagcacgag acaatcaagg aaactttcac cctgacttct ctccttatag
     9781 ttgggggcga gactacgctg aatgctctgc cattcaagct actttaggcg ttctccacga
     9841 catccctggc ttaatccaac tgatgggtgg aaaagaaacc tttagcaact atcttttgaa
     9901 agcttgtcaa gatgctcccc tctttgaaac aacaggctat ggttacgaaa ttcacgaaat
     9961 gagcgagatg gctactgctc cttttggaca aatagccatt tccaaccagc ctagtttcca
    10021 cattccttat ctcttccgct acagcgatta ccctgactac actgcccttc ttatcaagac
    10081 gctccgtcag aaagcttttc acccaagttg ggaagcctat cctggagatg aagacaatgg
    10141 tagtctctct gcttggtaca tctggtcagc tctcggattt tatccaacct gtccaggaaa
    10201 accaagctat gacctcggaa tccctctctt tgaccatcta cgagtctacc tcgctaaaga
    10261 agataaatgg ctggatatcc atactaaaca aaaccacaac cattttaact ttgtcaaaga
    10321 atgccgactg gacaaaacac tcgtatcaac tattcaacac caagacctct taaaagctga
    10381 acaactaact ttcaccctca gctggttacc aagtcactaa taaccaaaag aacctttgca
    10441 tcgcgcaaag gttcttcttt tatgaaactt ggacttgaag ttgatccaca agctgaccat
    10501 ccaccgttag gttcagatag aaatccaagg ttttatctcc tgcgaaatac agggttggta
    10561 gatgcaaatc ttttttgctt tcccctgctt ggaattgaag gacttgaatc tcatcctgat
    10621 aggcgacacc atggacacct gtccccggtt gaatcgaaat cttagcctct aaagaggagc
    10681 tactttcact acgttcaatc ctaaaatgaa gagtctctcc ctttgccaca gctaggcttt
    10741 tttctgcaaa tgctagttcc tgaacaactt ctttttttga taatccagga gttttacaaa
    10801 gggaaatctt ggtcaataca ggaatagatt gtgcctcggt aatcatcacg cgcacctttt
    10861 gtgcctctac tagcggacct cgcaagaggc gcttatgacc aactgtgaag cccctgccaa
    10921 attcctgcca gacaccatcc acctctactt gcacatgaaa agcagcgatt cgttgcccta
    10981 gcttcaaatc ttctcttaac tcaattacat caaaagtttt aggagaacct aaatcgagtt
    11041 ctaactggat gggcaagtct gcatcgcttg cccaagagct ggtctcaagg ccgtctgtca
    11101 aatggcgaca agcaaagtct gcggaaagag ctggaccaga tacctcagct cccagagcca
    11161 aatcttcttt atagagctca ttgcgatagg tcgcaaattc ataaagtcgt tcaatatcct
    11221 ttgcatcaaa gagcccagct tgattcggcg gaatattaag caagagtgga gttcctcgcc
    11281 ctactgagtg aaagtagatt tcgaccaact cctcgagaga cttaggatcc tgatcctcat
    11341 ggtagaacca gcctggacgg atggaaacat ctgcctctcc gatcgaaaaa atcgtgcccg
    11401 agggatcccc gtgctgaaga tagttcagct ctgcttctgt tcctagttta tcaggattca
    11461 tcttttgcca cagtggatca cctgcatgcc ctcgttcatt gccaatccag tggatactgg
    11521 tgccttctgt tgaaaaaatc aaacaatcgc cctgcaagtc acgaatggtt tcaaaccatt
    11581 tttcaaattc ataattaacc ttttgcgcgc cctctcctct ggcaccatcc atccaaacct
    11641 cagcgaactt accagcattc ccatagttag gatttgataa gatttccttc aactgagcca
    11701 gataataggc attgtagtcc gcttctcggt ccacatgata gaggggacta tgggcatccc
    11761 acggtgacag gtagaccccc atatccatat caaactctgt ggcagcttgg gatacttcaa
    11821 ggagcaagtc gccctttcct ctcctccaag gactgacctt aaccgaataa tctgtgtgag
    11881 ctgtcggata aaggacaaag ccatcgtggt gcttgaccac caaaatcaac tttttgaagc
    11941 ccgtttcctt gagcacacga acccactcac gcgcatccaa ctgactcggg ttaaagcgct
    12001 caggatcctc ctgtccagtc ccccattctt ggtcataaaa ggtattagga ccgaagtgga
    12061 taaaagctgc tagttcatct cccagataag ctagctgagt ctgacttggt aacggtccat
    12121 gcggtttgat tttcttcatc gttatatatc tccttcttta gcaacttctc ttattcacaa
    12181 ggttatccac aggttgtgga taataaacca tgatctaaca gtcagctctt tccaactcca
    12241 actggtaggc tcaggatttt tctcctcaat attccctctt caaacagctc tgtcatatgg
    12301 ttctgctaac acctctcaga atgctactct ccataattat tctcaggctt ctttcaggtc
    12361 attgtgtgaa gaccattcac tatccagact tttccacaga ttgtgaataa gtgattattc
    12421 taagctatca cctcgtcgac tataacaaaa aataaaaaga gggtcaataa gataattatc
    12481 tcttcctaaa tactcagaaa agattctata atttgtacaa ttgaacaaga aatgcttctc
    12541 caaaatctat cttcaaaaga atgttgcatc tttttatcct ttcacacaaa catttctctt
    12601 tatacagatc caagtgataa ccagatgata attggacact ttttggtcat ttgtatattt
    12661 tttgaaaaat tttttaaaaa aattttatat cctttttata gaagtttatg catttgta
//
LOCUS       DAVLTM010000052        12418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLTM010000052 DAVLTM010000000
VERSION     DAVLTM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.053"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            473..766
                     /locus_tag="VYN16_001247"
                     /pseudo
     CDS             473..766
                     /locus_tag="VYN16_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            873..1224
                     /locus_tag="VYN16_001248"
                     /pseudo
     CDS             873..1224
                     /locus_tag="VYN16_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1500..2806
                     /locus_tag="VYN16_001249"
                     /pseudo
     CDS             1500..2806
                     /locus_tag="VYN16_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2846..3856)
                     /gene="tsaD"
                     /locus_tag="VYN16_001250"
     CDS             complement(2846..3856)
                     /gene="tsaD"
                     /locus_tag="VYN16_001250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1403776.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3846..4283)
                     /gene="rimI"
                     /locus_tag="VYN16_001251"
     CDS             complement(3846..4283)
                     /gene="rimI"
                     /locus_tag="VYN16_001251"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1403777.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4280..4963)
                     /gene="tsaB"
                     /locus_tag="VYN16_001252"
     CDS             complement(4280..4963)
                     /gene="tsaB"
                     /locus_tag="VYN16_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1403778.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5142..5327
                     /gene="cibA"
                     /locus_tag="VYN16_001253"
     CDS             5142..5327
                     /gene="cibA"
                     /locus_tag="VYN16_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1403779.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKVIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5329..5481
                     /gene="cibB"
                     /locus_tag="VYN16_001254"
     CDS             5329..5481
                     /gene="cibB"
                     /locus_tag="VYN16_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1403780.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5804..6019)
                     /locus_tag="VYN16_001255"
     CDS             complement(5804..6019)
                     /locus_tag="VYN16_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403781.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            6299..6532
                     /locus_tag="VYN16_001256"
     CDS             6299..6532
                     /locus_tag="VYN16_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1403782.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDTSSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6534..8213
                     /locus_tag="VYN16_001257"
     CDS             6534..8213
                     /locus_tag="VYN16_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1403783.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8311..8388)
                     /locus_tag="VYN16_001258"
                     /pseudo
     CDS             complement(8311..8388)
                     /locus_tag="VYN16_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8592..10505)
                     /gene="mnmG"
                     /locus_tag="VYN16_001259"
     CDS             complement(8592..10505)
                     /gene="mnmG"
                     /locus_tag="VYN16_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1403784.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKIVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10515..10970)
                     /locus_tag="VYN16_001260"
     CDS             complement(10515..10970)
                     /locus_tag="VYN16_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1403785.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVREELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11111..12232)
                     /gene="mnmA"
                     /locus_tag="VYN16_001261"
     CDS             complement(11111..12232)
                     /gene="mnmA"
                     /locus_tag="VYN16_001261"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1403786.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKALSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTCDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3514 a         2919 c         2473 g         3512 t
ORIGIN      
        1 aaatttttat aatcatcaat aacctttgaa tctctagata cgattatcaa aataggatat
       61 agagacatcc ttctgatctg ctttttacaa agtccaatta tatgcggatc tatacctcca
      121 caatgtccat tattatacct aactataata tgagccgaaa acactatatc cttaatgtct
      181 ccatatccat cagggatatt aatatttatt tttccacaac tatattgcat tgtaaccatc
      241 tccttaaacg acgcattatg atatttgata gagaaatttt tatgaataac tcaataattt
      301 tatagtaaat catgcttata tctcaaagat acctattttc tcttgtctcg accttctcca
      361 aagaattgct ataatactat tacaaataca tctgcactac acttcaaatt ttagcactgt
      421 ataaaaacgt ttcaacacac taactttaag aaaacttcca ctattaattg aaaaagttga
      481 tagagataaa ttaaagatct atattgaaac tcatcccgat gcttatttga ctgaaatagc
      541 tgctgaattc aactgtcctc caacaactat tcattacgct ctaaaggcta tgggatatag
      601 tctaaaaaag agccgtacct actgcgaaca agacccagaa aaagtaaatc ggttccttaa
      661 agaattgaat cacttaagct acctgactcc tatttatatt tatgagacag gggttgagac
      721 ctatttttat ctcgaatatg atcgagcctt gagcaggcag ttagtctctc tggaagaaga
      781 tataattatt tgaattaaga tcgagacaac gcacaccaga gattgcgata ctgttataga
      841 agtactaatg cccttttttg tttcaatata ctatggctcc gatgacctat aaagatacga
      901 tgacgagtga ctttttcgaa gcttgcttcc aaaaattctt actacctact ttagatacac
      961 catcccttat cattatggac aatgcaaggt ttcacagaat gaacatgtgt aaggagcagg
     1021 gcatagactg ttaccacttc ctacctattc acccgagtat aatcccattg agaaaatatg
     1081 ggcttacatc aaaaacatct cagaataata ttgtcaaatt acgatgcttt tcttgaggca
     1141 cttttgtcct attcttgttt cagccgacta tactccgtta ttgggcagct acggaacagt
     1201 cgatgggacg atggggggac ataaaaaaat cctccagttt tgttttttat aacagtatac
     1261 tggagaattg acaatctcgg tagatacctc gttatagcgc ggttacttat taggcagtta
     1321 caaaacaact gtgaacagaa aacattccag agtcagacaa gactttggaa tgctttggct
     1381 ctataatttc tgtagtgggt aatcccaccc caggaattat agggtcgttt cttgtagaaa
     1441 aaaagcccca tatgacctat aatgaaaagc gtctaaccaa ctcattagaa agggtccata
     1501 tggaacaact taagaatacc acagatttgc tcggattgga agacaaaaat atcaaaatct
     1561 tgtctgttct gaaataccaa acccatctag tcgttcaggc aaagttggat tcccccgctc
     1621 ctccttgtcc tcattgtcaa gggaagatga tcaaatacga cttccagaaa gcctctaaaa
     1681 ttccgcttct cgactgtcag ggtttaccca cggtactgca tctcaaaaag cgccgctttc
     1741 agtgcaagaa ttgccttaag gtggtcgttt ctcaaacatc cattgtcaag aaaaattgcc
     1801 agatttccaa catggtgaga caaaaaatcg ctcagctcct ccttgaaaag cagtctatga
     1861 ctgagattgc ccacagattg gcggtctcaa cttccaccgt catccgaaaa ctgagggaat
     1921 ttaagtttga aaccgattgg accaagttgc caaaagttat gagttgggat gagtatagct
     1981 tcaaaaagag caaaatgagc ttcattgccc aagattttga gtccaaatcc atcctcgcaa
     2041 ttttagacgg gcgaactcat gcggtgattc gaaaccattt ccaacgctat cagagagagg
     2101 ttcgggagct ggtcgaggtc atcaccatgg acatgtacag cccttattat cggctcgcta
     2161 agcaactctt tccaaaggcg aagattgttc ttgaccgctt ccacattgtc caacatctga
     2221 gccgagctat gaaccgagta cgaatccaaa tcatgaacca atttgaccga aaatccttgg
     2281 agtatcgggc gctcaagcgc ttttggaacc ctcgcttttt cgtttctagg ctcgggctaa
     2341 atcagtccac tggactgatt tactacacca ggatagcttc aagctctgtc agaaacgatt
     2401 ctatcagtcc acgtttcgaa tgcacttaac ccatcgggaa gtacgagata agctgctttc
     2461 ctactctgag ggattacagg ttcactacga actctatcaa ctcctgctct ttcattttca
     2521 agagaagaat gccgaccatt tctttggatt gattgagcaa gaactgccaa cggttcatcc
     2581 gctttttcaa acggtctttt ggactttttt aagggataga gataagatta tcaacgcact
     2641 taagctgcct tattccaacg ctaaacttga agcgaccaat aatttgatta aggttatcaa
     2701 gcgcaaagcc tttggtttcc ggaactttaa caattttaaa aaacggattt tgatgacttt
     2761 gaacatcaaa aaagagagta cgaatttcgt actctccaga ttgtagcttt tcgcctaccc
     2821 actacacttg acaaagagcc actctttatt ccatggtatc aaaggcaaga cttggtttgg
     2881 cattgaggtc ccagcctgcg aagttttctt tgttccactc gctgacgctg gcataggcaa
     2941 tcatacctgc attgtctccg cagagtcgca gaggggggat gataaccttg acatctgtga
     3001 tttcggctgc taggcgttct ctgagacctt tattggctgc cacaccacct gccacaacca
     3061 gggttttaac aggatatttc tccaaagcct tcttggtttt tgccatgaga atgtccataa
     3121 ctgctgcttg gaaggaagca cacaaatctt ctgtagacag gctttctccc ttttgctcgg
     3181 cattgtgata aagattgata aaggcagatt tcaaacctga gaaggagaac tccagattat
     3241 cttccttaat catggcacgg gggaaatcat aaatatcctg cccctgatga gccagctcgt
     3301 caatctcacg acctgcagga taggtcaagc ccatgacacg gccgacctta tcataagcct
     3361 caccaaccgc atcatcacgg gtttccccaa caatcttata atctcctgcc tccgaaacat
     3421 aaaccaactc tgtgtgtccg ccgctgacca agagggctag caagggaaac tccaaaggct
     3481 ccacactctg agctgccatg aggtgcccag ccatgtgatt aacaggaatc agtggaagtc
     3541 cgtgagccca agcaaaggcc ttggcagctg acaaaccaac tagcaaggct ccgaccaagc
     3601 ctggtccgta ggtaaccgca acagctgtca cgtcctcttc ggtaatccct gcttctgcca
     3661 atgcctcctc gatacaggct gtaatgacct cgacatggtg acgactggct acttcgggca
     3721 ctacgccacc aaaacgtttg tgactctcaa tttgactagc aatgacattg gacaagagct
     3781 catcgtcgtt tttcaagacg gcgacactgg tctcatcaca ggatgtctca aatgctaaaa
     3841 tatatctatc cttcatctat ttctctcttc atgataatgg cgtcctcgac tgggtcatgg
     3901 tagtaggcct ttcgctcagc gatgaccgcc attttttctt tcttgtaaaa tgcttgcgct
     3961 cgttgatttg actttctgac ttcgaggaaa atctccttat ctgtcggcaa ttgagcaaac
     4021 aaggctgacg caattccctg accctgataa gctcctttga cagcgatttg caggacttct
     4081 gcttcaaaga gagtctcctg cacagctaga aatccaatca cttctgcccc atcataagcc
     4141 aatgcatacc aagtctggtc ttgggacaga tctgcttgga tttgctccag agtccaagga
     4201 ctgactaggt aaacagctgc cataacagcg tagatggctt gagctaggtc aggctgttgt
     4261 tgaattcgct tgatttctat cataggcgtt taatgtagga ctcaccagac tcggtatggt
     4321 tcttgagcca gttttcctct gcctcaacac gtttgaggta atttggcaca aaatcatgca
     4381 aggagtctgc ttccttgtcc caggccaaaa gagctagatt agctgcattg ggcaatgttt
     4441 ctttgtaatc agtccttggc aagtgttttt gaatctgctc aacaaagggg ccaacttctc
     4501 cgacaaaggt tacctgacta gcgcccttga ttagctcaat gactcgctca aaaggcagat
     4561 gtgcttctgc catgacaggt ttggcatttt cataaaatcc tgcataaaca ttattgcgac
     4621 gcgcatccat caaggggaca aacaaacctt cttgttggta gggcaccaga gccaagagac
     4681 tcgacatacc aaccaactcg atattcagag tataagccag agttttggca gtggctaccg
     4741 cgattcgtaa gcctgtatag ctacccggcc cttcagctac cacgattcgg tccaagtcct
     4801 tgggtgtcca atccaaactt gccatcaaaa aatcgatggc aggcataagg gtaatactgt
     4861 gatttttctt aatattaatc gtcgtctcgg caagaacctg cttatcctct aaaatagcca
     4921 gagaaagagc cttgctggac gtatcaaaag ctaatacttt cataacacat tcctatcttt
     4981 ttgtctgctt actattatac tacaaaagct ggcacatggg aattttcttt gcccccagac
     5041 aagagtgccc tcacttaact aaaaataatt taaaaaaaat gctcaatttt ccttttcttt
     5101 tccgaatata aaagtgaaca agaaaaaagg aggaaagttc aatgacaaat tttgacattc
     5161 ttgacaatca atttttatcc ttatctgaaa atgaattatc agatattgat ggcggtctcg
     5221 ctcccttagt tatctttgga gtagcagtat cttggaaggt tattgcaggt ggaacagcac
     5281 ttataggttc tggtttggca gctggttatt ttttaggagg agattaatat gatgaaagat
     5341 ttgaacaatt atcgtgaaat ttctaataag gaattgcaag aaatcaaggg tggctttggt
     5401 gtcggtgttg gtatcgcttt atttatggca ggttatacca ttggaaaaga ccttcgtaaa
     5461 aagtttggta agtcatgcta gataagaaac acatttttag aaggataaat tttattgtct
     5521 tcatctctta cagtttgctc agcattctca atgatttgaa cattactacc atccctttac
     5581 cattcgattt atctgtttgt attgttttat ttttatgctt caactctatt tttgatcaga
     5641 acaatgactc ccataaaaat aataagcttt gaaaattcca ttgtcatgtc atgttagaaa
     5701 aatgcaaaga ccacctcatc ttgatagatg gggtggaatt ttcgtgtcgt aaatctacta
     5761 tctctacatc cccaaacaaa aaaccccagc ataagcaggg catctaagca tttaattcaa
     5821 agtaaaatac aaaccaaacg acataggtca cgaggaggag aaaaagcgag tagagagtca
     5881 caaaggtcat tttccacaag aacttggttt gtcgtcgttc cagtttggca aatagaagat
     5941 tccccgcata aacgcaagca acaaaaacaa taaaagctac caagcgagct ccgatagcaa
     6001 aagcaaataa gttatacata gggcaacctc cttgacttaa aatctatatg gaattatgac
     6061 aagcaataaa tttcacttcc gttatcaaca taatacattt tctttgtttt tgcaaacgct
     6121 taccaaagaa atcgtcccct aactttctcg tttccgtctt ttactaattt ttcattttgt
     6181 ggtataattg aaataattgt aacgaatcaa ggtcaatcta gacacaaaat ggaatgaaat
     6241 caagcaaata tctgctaaaa gtttggaata agctgacctg taaatagaaa ggaactatat
     6301 gatttacaaa gttttttatc aagaaacaaa agaacgtagc ccacgccgtg aaacaacacg
     6361 cgcgctttac ttagatatcg ataccagctc agaacttgag ggccgtatca ctgctcgcca
     6421 acttgtcgaa gaaaatcgcc cagagtacaa tatcgaatat atcgaactct tgtctgacaa
     6481 attgctcgat tacgaaaaag aaactggcgc cttcgaaatt acggagttct aatatggcct
     6541 acactcttaa acctgaagaa gtcggcgttt ttgccatcgg tggtctagga gaaatcggga
     6601 aaaacactta cggaattgaa taccaagacg agattatcat cgtcgatgct gggattaaat
     6661 tcccagaaga tgacttgctt ggtatcgact atgtcattcc tgactactct tacatcgtgg
     6721 acaatatcga ccgcgtcaag gctgttttaa tcacacacgg acacgaggac cacattggtg
     6781 ggattccgtt cctactcaag caagcaaatg tccctatcta cgctggaccg cttgccttgg
     6841 ctttgatccg tgggaaactc gaagaacacg gcctcttgcg caacgccaaa ctttacgaaa
     6901 tcaaccacaa caccgagttg acctttaaaa atctcaaggc aactttcttt agaacgactc
     6961 actctattcc agagcctttg gggattgtca ttcatactcc tcaagggaaa atcgtctgta
     7021 cgggtgactt taagttcgac tttactccag ttggagaacc tgcggacttg catcgtatgg
     7081 ctgcgcttgg tgaagaaggc gtgctctgtc tcctgtctga ctcgacaaat gcggaagtac
     7141 caacctttac caactctgaa aaagtcgttg gtcagtccat tatgaagatt atccaaggta
     7201 ttgaaggacg tatcatcttt gcatcctttg cctcaaatat cttccgtctc cagcaggcaa
     7261 cagaagctgc tgttaagact ggacgcaaga ttgcggtctt tggtcgttct atggaaaagg
     7321 ccattgtcaa cggaatcgat cttggctaca tcaaagcccc taagggaacc tttatcgagc
     7381 caaatgaaat caaagattat cctgcaggag aagttcttat cctctgtaca ggtagtcagg
     7441 gtgagcctat ggcagccctc tctcgtatcg ccaacggaac ccaccgtcaa gtacaattac
     7501 aaccaggtga taccgttatc ttctcttcta gtcccatccc tggaaacact actagcgtca
     7561 acaagctgat taacatcatt tctgaagctg gtgtcgaagt tatccacggt aaagtgaaca
     7621 atatccatac atctggacac ggtggtcagc aagagcaaaa actcatgctc tgcttgatta
     7681 agccaaaata cttcatgcct gtccacggtg aataccgcat gcaaaaagtc cacgctggac
     7741 tagcagtgga tactggtgtt gagaaggaca atatctttat catgagcaat ggcgatgtgc
     7801 ttgcccttac tgctgactca gctcgtatcg caggtcattt caacgcccaa gatatctatg
     7861 tcgatggaaa tcgtatcggt gaaattggcg cagctgtcct caaagatcgt cgcgatctat
     7921 ctgaagacgg tgtcgttctg gcagtcgcaa ctgttgactt caaatcgcag atgattctat
     7981 ctggtccaga catcctcagc cgaggctttg tctacatgag agagtctggc gacttgattc
     8041 gccaaagcca gcgtatcctc ttcaatgcca ttcgtatcgc actgaaaaat aaggatgcta
     8101 gcgtgcaatc tgtcaatggt gccattgtca acgctattcg ccccttcctc tatgaaaata
     8161 ccgaacgtga accgatcatc atcccgatga tcctcacacc agatgaagaa taatactctt
     8221 caaaccacgt cagcttcgcc ttgccgtata tatgttactg acttcgtcaa ttctatccac
     8281 aacctcaaaa ctgtgttttg agctgacttc gtcagttcta tctacaacct caaagcagtg
     8341 ctttgagcaa cctacggcta gcttcctagt ttgctctttg attttcattg agtacaagtc
     8401 aacaaaacag ccccgtcttc ggagctgttt ttctctatgc tttcttttga gattaaaact
     8461 caatgaaaat cagactagaa ggctaagcga aagcataact tgagttagct cccatagttc
     8521 gggaaactat gggaggctgg agatgaatca aagccaagct ttgaactcat tcgtaagaag
     8581 ccgacgacgt atcattttga tttttgaaga gttttagaaa tactacgatt tttaccttcc
     8641 agatacacca tcaaaataga aatatctgct gggtttactc ccgaaatacg gctggcttgg
     8701 ccgatggttt ctggattgat gagtttgaac ttctgacggg cttcggttgc gatagaatca
     8761 atgtcatccc agtcgatatt ggccggaatg cgtttttctt ccatgcgttt catcttggca
     8821 acctggtcca tggctttgga aatatagcct tcatacttga tttctgtttc aatcaattcg
     8881 ataatcttgt catccaagtc ttctgcagct ggtccgatga aggccaccac atcttggtaa
     8941 gaaacttctg gacggcgaag gaattccttg gctgtcaccg catctgtcaa cggcttgaag
     9001 cccatttcct caaccttagc attggtttcc ttgactggct tgagtttgat actgtctagg
     9061 cgtttcatct cattatcaaa ttgatttttc ttgatttcaa aacgagccca gcgttcatcg
     9121 tccacaaggc caatctcgcg tcccatctca gtcaagcgca tatcagcatt gtcatggcga
     9181 agaataagac ggtattcagc acgactggtc aagagacggt aaggttcaat ggttcccttg
     9241 gtcaccaagt cgtcgatcat caccccgata taaccgtcac ttcgttttag aatcaattca
     9301 ggcttgcctt ggattttcaa agccgcattg atacccgcga taatcccttg gccagcagct
     9361 tcttcataac ctgatgttcc atttgtctga ccagcagtga agagacctga gattttcttg
     9421 gtttccaaag tcgcacgcaa ctgatgaggc aagaccatgt cgtactcaat ggcataacct
     9481 gttcgcatca tctctgcatt ttccaaacct ttgatggaat gaaccaagtc acgctggaca
     9541 tcctcaggta gactggttga gaggccttgc acatagactt cctctgtatt acgtccttct
     9601 ggctcaagga agagttggtg gcgttccttg tccgcaaagc gcacaatctt gtcttcaatc
     9661 gacggacagt aacgaggccc cactcccttg accacacctg taaacatagg cgcacggtgg
     9721 aggttgtttt ggataatctc atgactggta ccattggtgt aggtcaacca gcatggcact
     9781 tggtccttga cataatcctc atcacgtgaa gtgtatgaga agtgattagg cgcttcatct
     9841 cctggttgga tttctgtcac atcgtaattg atagaagaag ccttgacacg tggaggggtt
     9901 cctgtcttga aacgaccgat ttcgaggccc agttccttga gattgtcagc taggttaata
     9961 gaagccaagc tgtggttagg acctgatgag tacttgaggt ctccgatgat aatttcccca
    10021 cggagagcag tccctgtcgt cacaataaca gccttagcag catattcttg atgggtggct
    10081 gtacgcacac cgacaatctt gccatcttcc accaaaatct catcaatcat ggtttgacga
    10141 agggtcagat tttcttgatt ttcaactgtc ttgcgcatct ccttagagta aagttccttg
    10201 tcagcctgcg cacgaagagc acggacagct ggccccttcc ctgtgttgag catcttcatc
    10261 tggatgtaag tcttgtcaat agtcttggcc atctcgccac cgagggcatc gacttcacgc
    10321 acgacaatcc ccttggcaga accaccgata gagggattac aaggcatgaa agccagcatt
    10381 tcaatattga tggtcgcaag caggacctta cagcccatac ggctagcggc caaggaagcc
    10441 tcaaccccag cgtgtcccgc accaattaca ataatatcgt attcttcagt aaaatgataa
    10501 gtcatgtttc tctcctattc ctcaagatga atgtgtctta gttggccttc ccaatctggt
    10561 agggccgttt ttaaaaaggc tggaactagc tggatatcct caagcttatc caagtcaatc
    10621 cactcacagg gctgcctttt ctcatcttcc tgcatggtca acggggcatc ttcaagcaaa
    10681 tccaccagat aatgaaactc gatattgtga taggaaacgc cgtccacttc aaaacgattt
    10741 tcaaccacaa aagctagctg cccagcttga gctttaacac ccagttcttc cctcacttca
    10801 cggactaccg cgtcttccgt gctttcattg acttgaatcg cacctccaat agtgtaatac
    10861 ttgcccttgt ctttggtaac tagaagcttg tgattttgga caatcaaggc tgtcgcccga
    10921 acaccaaaaa ccgtattgtc tacttttgtc cgaaagtctt gttgagtcat tcttgtcctt
    10981 tcccttaaac gacacaaaaa cagtcaaaac tacaaagaag tgcaggacaa aaaagcctgc
    11041 aacatccatg agctttgacc atcatttcta ttgctgttat tattgtagca aattgagaaa
    11101 atttgtcaat ctaaatgtac tgacaaactt gtccatcgcg gtaagcattg tcaatcaaac
    11161 cgccacctag acactcttcg ccatcgtaaa agacaactgc ctgtcctggt gtaatggcgc
    11221 gttgtggttc cgcaaagatg acctctgcct tgtctccttt gacatggacg gtcaccttag
    11281 agtcaggctg acggtaacgg aatttagccg tacattctag cgtaaactcc tcgggcatat
    11341 cacaagtaaa gtggacttgg ctagcctcta ggctggttga catgagcgaa tcatggtaga
    11401 atccttgtcc tacatagaga atattcttgc ttagagcttt tccgacaacg aaccaagggg
    11461 cattgtcacc accgtgttgc ccaccgatac caagcccgcc acgctgacca atcgtatagt
    11521 acatcagacc tgcatgctcg cccatatcgc gaccatccac agtcatcatg cgaccaggct
    11581 gagctggcag gtagttgctg agaaagtttt taaagttctt ttctccgata aagcaaatcc
    11641 ctgtcgagtc tttcttctta gcagtcgaaa gacctgcttc ttctgctagt tttcgaactt
    11701 caggcttttc caaatgtcct agtgggaaca tggttttttg aagttgttct tgcgaaagtt
    11761 ggctgaggaa ataggtctga tccttgccat tgtccacgcc acgaagcatg tgaacggtac
    11821 catcctcatc acgcgccact cgagcataat gcccagtcgc tacatagtct gcccccaagg
    11881 ttatggcata gtccaaaaag gccttgaact tgatttcctt gttgcacata acgtccggat
    11941 ttggcgtgcg ccctgcacgg tattccgcta ggaaatactc aaaaacgcgg tcccagtact
    12001 ctttttcaaa attgacagag tagtagggaa tgccaatctg gtctgccacc gcaaccacat
    12061 ccttgtaatc ttcggtcgcc gtacagacgc cgttttcatc tgtgtcatcc cagttcttca
    12121 tgaagatacc gatcacatcg tagccctgct ccttgagcaa aagagccgtc accgacgaat
    12181 caacaccacc actcatcccc acgacaacac gtgttttaga gttatcactc atggtaagtc
    12241 tcccatctat tcgtttattc acgattgaag gtcgtgtgtg cttcacgatt gaaggtcgct
    12301 tgagcagtat tcattataac acgcttggtt agagaagaca aggaagagct taaaaattgt
    12361 tttttaaaac ttatagtcaa tatacagatt atgtgaatga tgtgagtata ccttattc
//
LOCUS       DAVLTM010000053        12042 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLTM010000053 DAVLTM010000000
VERSION     DAVLTM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.054"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(127..1470)
                     /locus_tag="VYN16_001262"
     CDS             complement(127..1470)
                     /locus_tag="VYN16_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1403787.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSNQAMLDGMET
                     FFSERSYNPVIEYMERAAEKWDGRNRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFIIARTTNQTEYLKDKTGER
                     RFLPIMADSRQQKKHPMEIDPDTIEQIWGEAVTIYRAGANLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKNSEKTNR"
     gene            complement(1473..2294)
                     /locus_tag="VYN16_001263"
     CDS             complement(1473..2294)
                     /locus_tag="VYN16_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEV1403788.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIDFADKPAMTPSEIE
                     NFWDGYPNANIALKTTNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKDLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPNPLPEKEMMRTIESMIKKDRR"
     gene            complement(2467..2970)
                     /locus_tag="VYN16_001264"
     CDS             complement(2467..2970)
                     /locus_tag="VYN16_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403789.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKDLNWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALQRKAVGSYYNLVISVTKGKDGREFRNYDLEEEAQPLTEADI
                     DEDDLPF"
     gene            complement(2990..3766)
                     /locus_tag="VYN16_001265"
     CDS             complement(2990..3766)
                     /locus_tag="VYN16_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1403790.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYVNH
                     VDDKAILKILATIRGAVEPAKTEKTKEAPKKEVASDDELF"
     gene            complement(3766..4083)
                     /locus_tag="VYN16_001266"
     CDS             complement(3766..4083)
                     /locus_tag="VYN16_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403791.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            complement(4102..5295)
                     /locus_tag="VYN16_001267"
     CDS             complement(4102..5295)
                     /locus_tag="VYN16_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEV1403792.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            complement(5258..5743)
                     /locus_tag="VYN16_001268"
     CDS             complement(5258..5743)
                     /locus_tag="VYN16_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403793.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            complement(5743..6102)
                     /locus_tag="VYN16_001269"
     CDS             complement(5743..6102)
                     /locus_tag="VYN16_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403794.1"
                     /translation="MELMNKTRVTDSLAVVIGPESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            complement(6102..6584)
                     /locus_tag="VYN16_001270"
     CDS             complement(6102..6584)
                     /locus_tag="VYN16_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEV1403795.1"
                     /translation="MASLYELTGQFLTIYQMDIDDETKTDTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELVRTEKLVIK
                     "
     gene            complement(6577..7299)
                     /locus_tag="VYN16_001271"
     CDS             complement(6577..7299)
                     /locus_tag="VYN16_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1403796.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTTYTKTQIGEVRRRLGLPVGKV
                     YQGKLLTKEQHDYLVSIQKNKISRDVANEMNLKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNGGQFKKGNRPPNYVPVGTINYTTDGYPKEKIGEPNQW
                     VLKHRKVWEEHHGPIPKGHSIVFLDGDKTNYDILNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            complement(7313..7543)
                     /locus_tag="VYN16_001272"
     CDS             complement(7313..7543)
                     /locus_tag="VYN16_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403797.1"
                     /translation="MVRNKLTDLTNTLFAQLETLDDRDLTADELKTELQRSKQMVAIS
                     SQILQAGQLALDAKKFKDKAGEDNAPIALLEG"
     gene            complement(7544..7885)
                     /locus_tag="VYN16_001273"
     CDS             complement(7544..7885)
                     /locus_tag="VYN16_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403798.1"
                     /translation="MTQAERIREYYREHPAASYDEVAEVVGTTNSNVRANLAKDIKAG
                     RCVRLEDKSYDYSPYYNHTQALTELVDWKNDIRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            complement(7897..8088)
                     /locus_tag="VYN16_001274"
     CDS             complement(7897..8088)
                     /locus_tag="VYN16_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403799.1"
                     /translation="MMEYIYLVIIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMN
                     LRNSDKQFTYDVEPPEGLK"
     gene            complement(8085..8279)
                     /locus_tag="VYN16_001275"
     CDS             complement(8085..8279)
                     /locus_tag="VYN16_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403800.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEK"
     gene            complement(8276..8548)
                     /locus_tag="VYN16_001276"
     CDS             complement(8276..8548)
                     /locus_tag="VYN16_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403801.1"
                     /translation="METVQIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            complement(8791..9327)
                     /locus_tag="VYN16_001277"
     CDS             complement(8791..9327)
                     /locus_tag="VYN16_001277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403802.1"
                     /translation="MESVLESLKQEKDHLEKIIKVVTSGGKFLRLPYQKKSRSISENL
                     KLISQNLDRLSCLYNQRGERMTDRELFKLPEDYVESTGLDKITFEVPFELFTKILKGY
                     GHKLAWEDFRQIKIHPSTRTKKTVGQCQFLFSIWMNDHLKPAIKPSKELQKEPVRKTK
                     KQKRLHKLAQNLLHRMKG"
     gene            complement(9359..9574)
                     /locus_tag="VYN16_001278"
     CDS             complement(9359..9574)
                     /locus_tag="VYN16_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403803.1"
                     /translation="MQWTLEAMRINKGLTQAELAEKFEVSSQTIARLEKDSSDIGYQL
                     LKKYMFFFNVKFDDIFLGKKYENFVND"
     gene            complement(9587..9772)
                     /locus_tag="VYN16_001279"
     CDS             complement(9587..9772)
                     /locus_tag="VYN16_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1403804.1"
                     /translation="MWEQLNRIMQERNLNGYQLSKMSGVNRSFFSDLKSGKVKYLSWT
                     NICKIADALEVSLDEFR"
     gene            9920..10111
                     /locus_tag="VYN16_001280"
     CDS             9920..10111
                     /locus_tag="VYN16_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403805.1"
                     /translation="MKSFKYFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            10328..11017
                     /locus_tag="VYN16_001281"
     CDS             10328..11017
                     /locus_tag="VYN16_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEV1403806.1"
                     /translation="MIFQVKVNSSVSRPVTVEDICPNCKKPTNPDLVNSSYFSLGENK
                     TSLVLTCRCLGCKHFWTEEFIATRFLINDYTEKYEIEHIKVIPNLPSDIPISDDVEIV
                     SPIGKQIYVQALKAEHEQLDHIAGIGYRKALEFFVKDFSIVTNPDDEDKIIKMSLKQV
                     IEKYIKDEDLKTFALASAYIGNDEGHYYRNNPDKGFTDLKNYLHGVIHYMEMKLNFLD
                     AQELVNRSKKS"
     gene            11279..>12042
                     /locus_tag="VYN16_001282"
     CDS             11279..>12042
                     /locus_tag="VYN16_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1403807.1"
                     /translation="MQIIAENITHFRKQRGITQKELAKEVGITASTMTDYMKLRSAPS
                     FGVIQKLADYFGVKKSDIDTTFKEESTNSLPDAPDSLTQQIMDKVVQLITPNKKIVLR
                     TSEELLESQKNEEETKVNEVSENIIRLDDYRQTTYRRVTGVVSAGSGSIQDDDLDMEV
                     SFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIGIFQVDGANYVKK
                     LRQGYLESLNPDYEDIHLDESNDIRTIGKVVSVYRE"
BASE COUNT         3284 a         2517 c         2056 g         4185 t
ORIGIN      
        1 taaaagtcag taatatcaag gggttagact gctaccgatg tgctaccgat gtctcatttt
       61 atcggtagaa tgctaccgat ctaccccctc aactgctacc gatgactacc gataattttt
      121 aaattgctac cgattagttt tttccgaatt cttcactctt acgaaccctt ttgtactttt
      181 acctcctgcc cggaaaacac tttttttcca atcaggatga ttatccatga tcatgttaat
      241 cttcgttgac aacttcctgt cattcgaatt tctcataaat aagttgtaca tcatttcacg
      301 agttgagacc ttatctagtt ttttgcttcc aggatcaaag tcgctactat tatcgaaata
      361 tttacttgtg tattgatgtt gttgctgaat agaccagttt tgccaatttt cagggacggg
      421 catatcaaga tattcaagca cttgtaattc aacttcatca cgatacatga actgttcacg
      481 gtagatattc aattcatcct ctgtattttc atcaaacatc aaattagcac cagcacgata
      541 gattgtaacg gcttcgcccc aaatttgttc gattgtatca ggatcgattt ccattggatg
      601 tttcttttgc tgcctgctat ctgccataat cgggagaaat cgacgttctc cggttttgtc
      661 cttcaggtat tctgtttggt tagtagttct ggctataatg aagtttttgg caaactcttc
      721 tgtgcgtttc atgtagggct tacgataccg taagctagtt tttgatataa aggctttcgt
      781 ttcagcaaag ctcatccgat tactggccac catttcatca tcgttgacga ttagactctt
      841 taacataatg tcgtaattat ctttattaga gaaatcagtc acggcatccg tataccattc
      901 gccacccaat ttttgaagga gggacgtttt cccaactcct tgtccaccaa ccagatccag
      961 aacatagtca aacttaacgt atggatcata aactttagca actgcaccaa ctagccacat
     1021 ttgagcgatt ttagaaacta aagggatatc ttcagcgccg agatatactt gaagcatgcg
     1081 gtcaatccgg tttctgccgt cccacttttc agctgctctc tccatatact caataactgg
     1141 attgtatgat ctttctgaaa agaaagtctc catgccatca agcatcgctt ggtttgagaa
     1201 agcaacacct aacacacttt caaagtaaac ttttacgact gaatcaaagt tagaagggag
     1261 ctcacctttt ttaaaaagag tgttgccgat cttgatatct ttaaggagtt catgctcttg
     1321 ggaaaaatcg ttgtgcttta ggtaaatact caactgatca tcagctttga aagacatcaa
     1381 cacattactt ggactgttgg ccttgatgtc tcccttggca gtcgttatca tcttaggttg
     1441 tgagtcaata cttactacat taccaatcac aatcacctcc tatctttttt aatcatactt
     1501 tcaatagtac gcatcatttc cttttcaggc aaagggtttg gactatttgc atttgctaat
     1561 cttgcaagtt gaacaactac accatcgtcg accgcacgat ataagagacc acctacgaat
     1621 tttgctagtt tgtcatttcg tccaccttca tcaccaaaac caagggcgat ggtttcaaag
     1681 agatctgtag tctgtgttcg gtctctagta tgtgaacgtc taactaaatc cctaagaccg
     1741 tccttaccat catacttata gccgtgagtt tcgccatact gtttttttat agactggatt
     1801 aaatcttttg aaggagtgac catcgtgcca ccttccttag acttttccag atcccactca
     1861 tactgccctt tatctgtggc agagggtgcg actaacacat aattattttc gtgagcctta
     1921 atatcaacac caggcaagaa tccaatcatc tgagtgatcg gctcatcttc tcgtttgaag
     1981 tagaatagat gtttaccgcc actagccgtc ttagcttgca gtgtcggttc gattaaattt
     2041 agatgtttcc atttttttag cgattcaaaa ccgttcgatt tgccgtgttt gtcaatatca
     2101 atgacaaaga agttggtagt ctttagggca atgtttgcat tagggtagcc gtcccaaaag
     2161 ttttcaatct cagatggagt catggctggc ttatcagcaa aatcaatcaa aggcatcttg
     2221 tttttaggat tgattggaat gactgagaac cctaactttt gatactgtaa tgcgtattct
     2281 ttcatcgacg gcatgattac ttctcctctt tgtaaatata aacaagttct tgggccatat
     2341 aatttgattg atattcatct tcagtcgttt ttaaataaaa taacaacgat tgataagcct
     2401 cttcaaatgt attgaatggt cctaatcttt catcagtttc atcaatgacc caaaacttgc
     2461 tattttttag aaagggaggt catcttcatc aatatcagct tcagtcagcg gttgtgcttc
     2521 ttcttcaagg tcatagtttc ggaactcacg gccatctttc cccttagtca cagagataac
     2581 aaggttgtag taagagccaa ctgccttacg ttgtagagcc tcttccaagg ctttaccgtc
     2641 ttcttcattt ccttgcatac tgtcgccagc aaggaccaag gctttgataa agaatttcat
     2701 ggtgcgttca actgcccagt taaggtcctt accgttccat tcagtcagtg tgccaaatgt
     2761 tgcaaattca gagcgtccac tgtaatcacc gccacggatt tcaaattgat aaccaaggct
     2821 ttcccagcct ttgtccgata cgttgaaggt tgctttcttc aggactactg gataagtacc
     2881 agctgggatt ggtgcaggac cgttggcgct gtctttgcgt gggtcaaagc cctctttttt
     2941 gattgatttt gcgatatcta gtaagctcat gtgtattctc ctttatttct taaaatagtt
     3001 catcatcaga ggcaacttct ttcttaggcg cctcttttgt cttttcggtt tttgcaggtt
     3061 caacagcccc acggatagtt gctaagattt tcaaaatagc cttgtcatca acatggttca
     3121 catagtaggt cttacgcttg cggtcaacct cacggttgta gttgttgccg agtttttcag
     3181 tgtggatcat caaatcagag tttccattga taagattgac atacttatct ttcaagcttg
     3241 gtttgtcttt ggtggcattg ccattgtcat catattcaga tacctgacgg ctgatgtaaa
     3301 taacattcat tggcaatgct ttgaggtcaa tgactaattc tgtgatagct tggttaaaga
     3361 agtcgtatcc tttgccgtat ggaatttccg acaaggattt caagcgaggt ttaccaactg
     3421 gggttaattc atcacaaact gcaatcttaa tcatttcaat aacatcgtca attacatcaa
     3481 taacgactgt ttcataagag tgcttctgtg tctggagagc aagcaagata tctccaagct
     3541 gcttaattac tgaattggta attcgtccct tgtcatcttt ttcattgatc agctgaatgc
     3601 ttggaacagt gttagcttct gcattcccgt ctgtgttcaa aacgattgga tttgggaatt
     3661 catttgcaag ataagacttt ccgctcatgg tttcaccgta gatgaaaaaa tttcgtgggg
     3721 tatctttagg aacttgtggt ttatttgctg gaagtgtaaa tgccattaga gaacccctcc
     3781 aataatatcc ttaatcatgt cctcaattga tgaacggtca cgcttgatag gttcaacttc
     3841 tgatccattc ggataggtca atttgtattc cgcttcaact gcgacaattt cacagtcaaa
     3901 agctgcagca agagccttgt aagtcttttt gttgtcttcg tattttttac gaggaagctt
     3961 caaacaatat tccaagctac aaaagtcagc tgcaaatgct aaagaacttc tgtccttgta
     4021 agaattaaga aattctccag ttttacggct acgaaatacg atcatttcag ttgttttatt
     4081 cattttgttt tcctcttttt tttaactttc tttataataa aattcaatta catttacatc
     4141 atgctgctga cgacttcctg ttatgcgcca gagcaattgt cgataatcat catattctcc
     4201 agaaccttct tctactggat ccaatacgac aatagtttgg tatttgtgtt gcaagccgtc
     4261 tactccgaca cctaacactt gactagtagc aaccacgaat ttcttataaa gtccttcttg
     4321 aatatcgccc gtccagattc caatttcagg atggcgctcg ctaatgacgt tgacaatctg
     4381 cttagattta ctgacaatca acatatcgtg tggtgctcgt tcgattaaac cgtcaagttg
     4441 taacatcaat ggcgtatccg catttactgg ctttaatttt ggaaaatcga ctgctacgcc
     4501 tgtttgatta aggtagcgtt caaaggtctt tcttccaaac gattgcttgg ccattgcagt
     4561 tttgccgtcc actgttacaa gatttagttt tctaaattct gcaagttttt ctggattgcc
     4621 agaggcgact ctcttttggt aaaacttaat ctcaaaaccg ttattctcaa ctgcattctc
     4681 aattccttca atctcttccc agcggaagaa attaggcaag ctcgagatat aactttcata
     4741 attttgaaaa tcttcccact tctcttttga atagctaaat ggatcataga ccatttttcc
     4801 atgagtcttt tgccagtcaa atttattatt tggggttgcc caaccaaata ccgttttttc
     4861 aagcggatag aaattttgtc cttttttccg aattggtgtc gctgaaagac ctatcgtgta
     4921 ttttcgcttt attttgcgat ataaggccac ttgtttgtca gaagacatat tctgccattc
     4981 gtctactatc agcacatcac aatctaattt atgccccttt ttgacttgat tttgaagata
     5041 tctatctgtc tgaatgataa tctcaacacc tttatcaaaa ttcataaact tgactgcatc
     5101 tatccaacca ttcagaatag ctagtcgatt gtttgtgatg atgatttttt tagctttttt
     5161 atgttttgca atagcaagtg cacagatagt tttgcctctg cccccaagag cctctaaaaa
     5221 gattccatta gataaatgtt cacttctttt aatcgcttca gcttgccact ttcttagcgt
     5281 tattgtgata ctcactcacc acctttccga tatcatgaac cacttcttca atatcatttc
     5341 tcattgccca aaataatcca agtcttgctg ctgctcgaat atcttgatga tgactttttt
     5401 caaatttcca aaggtctaag attttcaaaa gatcgtttgg aatatccgac ttgtaacctg
     5461 cattgaattg aagaatggca cctggatagc aaagttggat ataggcgatg gtttctgcca
     5521 cgctattatc tttcgacttg tcgttatccc tcgccttaaa ttcttcaaca ataactacat
     5581 cgaattcaag atttgttccg atttcgtgaa accaatcagc gaaacctctc ataccataag
     5641 agacaaccca gctatcaact aatcttgcat tatccaacaa gacaactcct gttgtgctgg
     5701 tttcaatttt attactgctt ggatcaatag ctaaaatttt catcaaacac caactttctc
     5761 agtcagcact cctggataaa gggcagtgtt aaaccaattt tgtttattta cctttgcaaa
     5821 ggcaaatagc gatttaactt cttttgcttg cttctcaaat tttcgaatat cttcctccga
     5881 ttcaaagata ggtttttcct tgtatttagc aactgtgacc agcttgtatt ccggagtgaa
     5941 tactggcttt tcatttcctt gatcaagatt ggtttcgtct acttttacaa aacgaatcgc
     6001 aacatcaaat agaaaacctt cagtaacaag tacttcaatc gattctggtc caatcacaac
     6061 tgctagtgaa tctgttactc gtgttttatt catcaattcc attacttaat caccaacttt
     6121 tctgtccgga caagttccgc acctttgact ttcttgccag atttaagcaa ctctttgagt
     6181 gtttttttgt ccggcgcaag cgtcactttt tttgtaaaat attttttcgg aaggtcgtct
     6241 tcgttgacct tgactgattc tggattctta gcaattttta taatcagggc accactctta
     6301 acttcggttt gccccgtgac attcatagct gccataatgt tatcctttac ataatccagc
     6361 tttttctgtg ccacctgttt cttggctttg aagctctctt cctcagcctt gtacatggcc
     6421 acgtcggctt ctagattctt gataacatgg gcatatcctt ctgctttctg ttcgaattgt
     6481 tcttgccaat cgatagcctc aagtgtgtcc gtttttgttt cgtcatcaat atccatttga
     6541 taaattgtca gaaactgacc tgtcagttcg tataaactag ccattttttt ctacctctct
     6601 gattttgttt gtgagttttg ttagtccaat acctgatttg gttaaatcag cgttggacgt
     6661 aaataaatga ttttgattca ttctagcaat ttcgttttta gataaacatg ccaggtttaa
     6721 aatatcatag tttgttttat caccgtccaa gaagacaatc gaatgccctt ttggtattgg
     6781 cccgtgatgt tcctcccaaa ccttgcggtg tttcaaaacc cattgattag gttctccaat
     6841 cttttctttt ggataaccgt ctgttgtgta gttgatagta ccgacaggta cataattcgg
     6901 aggtcgatta ccttttttga actgcccgcc gttttttggc atattggggt acttcttccc
     6961 cttattgtga ggagtctgac ctttctcgaa tcttcctgtc aaaccactat gtagattatt
     7021 atttcttcga taactcttaa tctgtttctc agtcagtgat aatccaaatt ttaggttcat
     7081 ttcatttgcg acatcgcgag aaatcttatt tttttggatt gacacaaggt aatcatgttg
     7141 ttcttttgtc aacaatttac cttgatatac ttttccaaca ggtaacccta aacgtctgcg
     7201 tacttctcct atttgagtct tggtataggt cgtaccaaat ttctcattta gtaacctggt
     7261 tacttcagga gttaatcggc cagggcatat ctcatgcatg tactctgtat actcatcctt
     7321 ccagcaaagc gatcggggca ttatcctcac ctgccttatc tttgaacttc ttagcatcca
     7381 gggcgagctg tcctgcttgt aagatttggc tagagatagc gaccatctgt tttgaacgtt
     7441 ggagttccgt ctttaattca tctgcagtaa gatccctatc gtccaatgtt tccaactggg
     7501 cgaaaagagt attggttaaa tctgtcaatt tatttcgaac catctacttc gtcacctctt
     7561 tcatcaattt atttgcttct ttgattaaca aacgcataac attgctatcc gtttcttttt
     7621 ctgctgctct tgtcagcata tccacccact cacgtctaat atcattcttc caatcaacca
     7681 actcagtgag tgcctgtgta tggttatagt aaggcgagta gtcgtatgac ttatcttcca
     7741 agcgaacgca tctacctgcc ttgatgtctt tggccaggtt cgctctcaca ttactatttg
     7801 ttgtaccaac gacctcagcc acttcatcat atgaggcagc agggtgctct ctataatatt
     7861 ccctaattcg ttcagcttga gtcatgtttc tcctccttat ttcaaccctt caggcggttc
     7921 tacatcataa gtaaattgct tatctgaatt tctcagattc atccgtgcaa cattgtttgc
     7981 catcagctgg cgctcttttt gtttcatttc agcgtggtca tctagtttat ttactagcga
     8041 ccatagtcca attcctacga ttattaccag gtaaatgtat tccatcattt ttctttctcc
     8101 ttttctttgt agattgctac gattttcttc aagtctgcga tttcttgatt cgcttcttga
     8161 agtttttcct gtgtttcaat tagtgattga ttgaggtcta aagcgacctc tttccagtcg
     8221 agattagttt ctttgacctc ttccgaaaaa tagtttttga ttcttgatag taggttcatc
     8281 ctgctgacct cattttcttg cttgtttcca tttctttttt ccaagcttta gttccacgat
     8341 attgcaagta ttcatagaaa cctttaatcg ttacaagttg tccactatcc aaaagatgtt
     8401 tttgctgact agggagtttt tgcatttctc ttcttcgctc tcctgcttgt cgttttgaac
     8461 atccaaagat acgttttaac tcttcatcat tagcagagac tttttcaatg atcacgtctt
     8521 taattctcac gatttgaact gtttccattt ttgctccttt cgtgttataa tttagttagt
     8581 tatttttgat gagcgcctga cttcgttagg tgcttttttc tgctactcaa tcttcttatc
     8641 ctttcttctc tcgttttctg ctataataca tacagaaagg aggataactt atgactaagt
     8701 ctgatttggg accttatcta gatgcagtta caaacgaaga tggtactctt ctaatctgca
     8761 aaacagagca aggtgcatac attggcgatt tcaacccttc atgcgatgaa gaagattttg
     8821 tgctaactta tgaagacgtt tctgtttctt tgtcttacgc acaggttctt tctgcaactc
     8881 ttttgaaggt ttaatcgctg gcttcaaatg atcattcatc caaatactaa acaaaaactg
     8941 acactgtcca accgtctttt ttgttctagt gcttgggtgg atttttattt gtcggaaatc
     9001 ctcccacgct aacttatgtc cgtacccttt tagaattttc gtaaacaatt caaaaggtac
     9061 ttcaaatgta atcttgtcaa gtccagtaga ttctacataa tcttctggta acttaaatag
     9121 ttctctgtct gtcattcttt ctcctctttg gttatataaa cagctcagtc tatcaagatt
     9181 ttgagaaatc aatttcagat tctcactaat cgagcgtgac tttttttgat acggcaatct
     9241 cagaaattta ccaccagagg taactacctt aatgattttt tcgaggtggt ctttttcttg
     9301 tttcaagctt tcaagaacgc tttccatcta tgtgttcctc cttcttattt ttttgtttct
     9361 agtcatttac gaaattttcg tattttttac ctaaaaaaat atcatcgaat ttcacattga
     9421 aaaaaaacat gtattttttc aatagctgat aaccgatatc agagctatct ttttctaatc
     9481 tagcaattgt ttgacttgaa acttcaaatt tttctgccaa ctctgcttga gtaagtcctt
     9541 tgttgattct catagcttct aaagtccact gcacgttcct acctccttat ctaaattcat
     9601 ccaagctgac ttccaatgca tcagcgattt tgcatatatt agtccaagaa aggtacttta
     9661 cctttccact tttcaaatca gaaaagaaac tacggttgac tccagacatc ttagataatt
     9721 gataaccatt caaatttctt tcctgcatta ttcggtttaa ttgttcccac attttacacc
     9781 cccaaaaaca ctatatgttg ttaaacaaat atatttaatt aacaatatgt tgtgtttttc
     9841 tgttatctat gttataatca ttcttgacta agacctctca ccgttttagt caaaattcca
     9901 atagaaagga ggagatttta tgaaatcctt taaatatttt cgagaatctt taacagctga
     9961 agatatgcaa gctatctctg ctaaagctaa tgaagctact aaacagattg accatacaga
    10021 cggattgcaa ctggggaagg tcagtggttt gacttctgta ataactacta ttgagttact
    10081 tgagaagtat catgaatggc ttcatagcta agacgcttaa attcttctaa gtctatctga
    10141 aaattgatag gctttttttg taaacactca agaaaactag tgtttcttaa aagtttttca
    10201 actaactcag ggtcggcctt tacaaaggtg gactcttttt tcccactata cggatatcgt
    10261 cttggtctca tttcctcacc ccctttcaaa tatggtataa tcaaaataaa acgattggag
    10321 aaatcttatg atatttcaag tgaaagtaaa ttcttctgtt tctaggcctg taaccgttga
    10381 agacatttgc ccaaattgta aaaaaccaac caatccagat cttgtgaact cttcttattt
    10441 ttctcttgga gaaaataaaa caagcttagt attaacatgt agatgcttag gttgtaagca
    10501 cttttggaca gaggagttta tagctacaag atttttaatc aatgactata ccgaaaaata
    10561 cgaaattgaa catatcaaag taatccctaa tcttccgagc gatataccta tatctgacga
    10621 tgtagagata gtttctccaa ttggtaagca aatctatgtc caagcactga aagctgaaca
    10681 cgaacaacta gaccacattg caggtattgg ataccgtaag gcgcttgagt tctttgttaa
    10741 agatttctcc attgttacaa atcctgatga tgaagataaa atcattaaaa tgtcattaaa
    10801 acaagttatt gaaaaatata tcaaggatga agaccttaaa acatttgcgc ttgcatctgc
    10861 ttatatcggt aacgatgaag gtcattacta tagaaataat cctgataaag gttttacaga
    10921 cctaaagaac tacctacacg gagttattca ctacatggaa atgaaactca atttccttga
    10981 tgctcaagag cttgtgaatc gttcaaagaa atcttagaat ctacttcatc caacttctca
    11041 gctatatatg tcacagtcct caatatctca ttgagggctg ttctttctag ttcgttcatt
    11101 tgtcttacct ccttttcctt aagcttgatt taattataat acgatttttt cgtattgtca
    11161 atagttttta tcaaaaaaat aggatttttt cgtatttttg attgtttatc attcaaaaat
    11221 gatatataat gtaattataa aaaatacgag gtaatcgtaa atggacgaaa aaaaacgaat
    11281 gcaaattatt gctgaaaata ttacacactt tagaaagcaa cgtggcatca cccaaaaaga
    11341 gttggctaaa gaagttggaa ttacagcaag tactatgaca gactatatga agttaagaag
    11401 cgctccttct tttggtgtta tccaaaaact ggctgattat ttcggtgtta aaaaatcaga
    11461 tatagatact acttttaaag aagaatccac caactcactg ccagacgctc cagattcgct
    11521 cacacagcag ataatggata aggtagtgca attaatcact ccaaacaaaa aaatcgttct
    11581 tcggacgtct gaagagcttc tggagagtca aaaaaacgaa gaagaaacga aggtaaacga
    11641 agtatcggag aacatcatca gactggacga ctacagacag actacttacc gacgtgttac
    11701 tggggttgtc tctgctggta gtggttcgat acaggacgac gatttagata tggaggtttc
    11761 gttctatgag gatgaaatcc cagacgacta cgacgctatc gcttatgtcg tcggcaactc
    11821 tatggagcca aagataaaga atggcgacta tctttttata aagaataccc aacaggttga
    11881 ttataacact atcggcatct tccaagtaga cggcgctaac tatgttaaga aactgcgtca
    11941 gggatatctg gaaagcttga atccagatta tgaggatata cacctagacg aaagcaacga
    12001 tatccgaact attgggaaag tcgtgagtgt gtatagagag aa
//
LOCUS       DAVLTM010000054        11850 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLTM010000054 DAVLTM010000000
VERSION     DAVLTM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.055"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..468
                     /locus_tag="VYN16_001283"
                     /pseudo
     CDS             1..468
                     /locus_tag="VYN16_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            428..1609
                     /locus_tag="VYN16_001284"
                     /pseudo
     CDS             428..1609
                     /locus_tag="VYN16_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678337.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1872..2264)
                     /gene="rpsI"
                     /locus_tag="VYN16_001285"
     CDS             complement(1872..2264)
                     /gene="rpsI"
                     /locus_tag="VYN16_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1403808.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2284..2730)
                     /gene="rplM"
                     /locus_tag="VYN16_001286"
     CDS             complement(2284..2730)
                     /gene="rplM"
                     /locus_tag="VYN16_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1403809.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            3086..3403
                     /locus_tag="VYN16_001287"
     CDS             3086..3403
                     /locus_tag="VYN16_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1403810.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(3448..4260)
                     /gene="folK"
                     /locus_tag="VYN16_001288"
     CDS             complement(3448..4260)
                     /gene="folK"
                     /locus_tag="VYN16_001288"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1403811.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLET
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(4303..4857)
                     /gene="folE"
                     /locus_tag="VYN16_001289"
     CDS             complement(4303..4857)
                     /gene="folE"
                     /locus_tag="VYN16_001289"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1403812.1"
                     /translation="MDTQKIEAAVKMIIEAVGENVNREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(4838..6160)
                     /locus_tag="VYN16_001290"
     CDS             complement(4838..6160)
                     /locus_tag="VYN16_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1403813.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMVDY
                     QSLLEGGVASNFQETTEFEIITALAYNYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEVIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLNYQD
                     WHDFLDQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(6162..7142)
                     /gene="folP"
                     /locus_tag="VYN16_001291"
     CDS             complement(6162..7142)
                     /gene="folP"
                     /locus_tag="VYN16_001291"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1403814.1"
                     /translation="MVKSLEGVTDMSTKVNHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAEVVAKAGAKVVIMFNPVIARPQHPSS
                     LIFPHFGFGQAFTEEELANFEKLPIEDLMETFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHKMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(7237..7944)
                     /locus_tag="VYN16_001292"
     CDS             complement(7237..7944)
                     /locus_tag="VYN16_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403815.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSIVLSWTAYKTQRLEMSILLHMIVNGVAF
                     CLLALVVIMSRTLGISV"
     gene            complement(7997..9415)
                     /locus_tag="VYN16_001293"
     CDS             complement(7997..9415)
                     /locus_tag="VYN16_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1403816.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            9605..10417
                     /locus_tag="VYN16_001294"
     CDS             9605..10417
                     /locus_tag="VYN16_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1403817.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            10756..11775
                     /gene="adhP"
                     /locus_tag="VYN16_001295"
     CDS             10756..11775
                     /gene="adhP"
                     /locus_tag="VYN16_001295"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1403818.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIATADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3676 a         2566 c         2226 g         3382 t
ORIGIN      
        1 ttaaaatatt ttagatataa tttagattat tacctaatta tcagagaaag cttattgtat
       61 gtattacata caaaccgcct ttatacaaca cataaagata gctcaggata tgaaaaaatc
      121 tctaggagcg caatcttaca atacgagtta gacaaacaaa aggggcatat ctttatcaaa
      181 gctctgatta ctggtgatga atttagtaaa agggtacaat cacttgatga tgttcttgct
      241 ggagtactcg aactggaact agaatacaaa attattcgtc ctagttttat ggaataccat
      301 ttctattaca agaaacctga caggttagtt cttcaatctc acaaccagaa gcaagagata
      361 gataacctta atatagattt aggatacggt gtgaattata atcctatcaa gtgtcctcat
      421 attctagttg ccggaggtac aggaagcggg aaatcagtat ttatatcttt aaggaaatca
      481 ctttgttaaa cgctataaaa caagaaagga ggctaaagaa gctgaattag aaattctaac
      541 aaagatacat caacttgaaa atggagagga tatcccactc cttaaaacta gtgatattct
      601 tttctctgag ttctttaata atatttggtg ggattcatac aaagctggtc aaacaacttc
      661 aacaactaaa cccccaacac aagttactat tgataatact gaaatagttt tcaggaagca
      721 catcttacca atgttcggta attattcaat caattttctg aatcagaata agcaaatcgt
      781 actaaactta atgactgcta aagctgaaga atatgctaat ttcaaggtta ttcgaagcta
      841 tgtaaactca attttcgatt gggctgaaga attagaatac attgaatcaa atagattatc
      901 aaaaagcata aaacgtatta aagcaagtaa aaaaataaag ctagaagaag ctaaggatga
      961 ggaagatttg tatctatcta gtgaagaatt acaagattgg tttgatgctt ttagagaaga
     1021 tttggagaac gacaaattat cactaaaaga ttatgtccta ttctttacaa cctttatttt
     1081 gagcgataga aaatcagaat cctatgctct acattggaaa aacatagact tggcaaacgc
     1141 tcaaattggc ttaaggcatg cacttgacaa atataagaat gtaaagtcaa caaaaggaaa
     1201 taagaaaacg attttttcaa ttcctagtta ccttgtatca cttctttcag aatggaaaaa
     1261 acagcagaaa tatgagttag cgaaatttgg tataatgcaa acatccgatc aactagtctt
     1321 tacctatata gatacgaaag gtaatgtaaa tagccctcta cacgttgatt atctcaataa
     1381 caaaatgaac tctgtaaaaa gacgacaccc aaagttaaaa cacgctacac ctcataaatt
     1441 acgacataca ggagcgacac ttgctaaaaa ggcaggaatg agcttagaag ccatttctga
     1501 agtattaact catagtgata caattacaac taaaacttat gttaatgctt caaacatagt
     1561 tccattatct gctggacaag ttgcctacca acatcttaaa aacaagtagg gtaattttag
     1621 ggcaaatttt agggaaaact tttttagaaa gtatagaaaa aagcacccat agggcaaacc
     1681 tttgagtgct ttgaaacatt gatataatcg tattgattaa cgttttgagg caactgacct
     1741 tgtcaggttg cagccacatt tgtaagcgac taaagtcgca acaactgtgt caattgcacc
     1801 aatttagtaa gaaagtataa aaaaagaaca ccccgaaagg tgctctgtat aagtatagta
     1861 attctttcga attaacgttt actaaattgt gatgctttac gagctttctt aagacctggt
     1921 ttcttacgtt caactttacg tgagtcacgt gtaagaagtc ctgcgcgttt caatgaatcg
     1981 cggaagtctg ggtctacttg aagaagggca cgagcgatac cgtgacggat agctcctgat
     2041 tgaccagcgt atccaccacc tacaacgtta acgaaaacgt cgtatgaacc tacagttgaa
     2101 gtaactgcga atggttggtt gatgacaaga cgaaggtcag cgtgtgggat atactcttca
     2161 acatcttttt tgttaacagt gattttacca gttcctggaa caaggcgaac gcgtgcaaca
     2221 gcgtttttac gacgtccagt acctgcatat tgtgcttgtg acatacttta tttgttcctt
     2281 tccttagata agtcctgaaa tgtcaagaac ttctggttgt tgtgcagcat gagtgtgctc
     2341 agctccaaca aatactttca acttcatacc ttgagcgcgt ccaagtgtat tgtgtggaag
     2401 cataccttta actgatttct caatcaaacg tactgcattt ttagaacgaa gttcacctgc
     2461 agagatttgt ttcaatccac ctgggtggtt tgagtgagtg tagtagattt tatcagttgc
     2521 ttttttacca gtcaatttaa ctttttcagc attgataaca atcacgaagt cacctgtatc
     2581 agtgtgtggt gtaaatgttg gtttgttttt tccgcgaagt acgctagcaa ctactgcaga
     2641 aagacgtcca agtggtacat cagttgcgtc aactacgtac catttacgtt caacttggcc
     2701 tggtttagcc ataaatgttg ttttgttcat gatttctcct atatatagtt cgatttttgt
     2761 ttacggtgat gggtgttcct tcccatcgaa aagtatttgg aaggttccgg ggcctttcaa
     2821 atggggtaaa caataccgcc tactatcata ccaaatttca cccctaaaag tcaatagata
     2881 taaaaaatat ttttcggtaa cttctcaaaa taactgaaag ttatataatc aagttaattt
     2941 gggaatttac ctattaactt cggtagattt ttgggaattt tttccctatt tctccctgta
     3001 actaaaatcc ttttcaaaaa atcacctttt cttgattttt aatcctattt gctgtatgat
     3061 aagggaaaag aaaggggaca gagatatggc ttttaccaat acccacatgc ggtctgctag
     3121 ttttggcatt gttaccagct tgcccgatga catcattgac tctttttggt atatcatcga
     3181 tcatttctta aaaaatgtct ttgaattgga agaagaactc gagtttcaat tgcttaataa
     3241 ccaaggaaag attaccttcc acttttcaag tcaacacctc cctacagcca ttgattttga
     3301 ctttaaccat cctttcgacc ctcgttatcc cccaagagta ctggttttag acatggacgg
     3361 tagagaaact atcctcctcc cagaagaaaa tgacctattt taaaaactct agccttcagt
     3421 tgcaagtaac tgaaaactag agtttttcta ttttttcaaa gcatcataca agttgcggat
     3481 cggttgtttt aatatcggat ggataaaatg aggcgcaatt tcctgtaagg actcaaggac
     3541 aaaaaggcgt tccgctatgt aaggatgagg caatatgagg tcgtctgtat aaaggatctg
     3601 gtcctccaca aagagcaagt ccaaatcaat caaacgaggt ccccaatgca cttctctcac
     3661 ccgtcccagc tctgactcaa tggctaacaa ggtttctaac aagtcttgtg ctggtagcca
     3721 ggtttccacc tcaaccactt gattggcaaa gctatcctgc tccactccac cccaaggctc
     3781 cgtctctaag acactggact ctttgagaat atggatgcca cgagctcgca gtttgtcaat
     3841 ggcttgcttc aagtttactt gtttatctcc catattgctt cctagggcga taaaggctcg
     3901 ttgcttgcgg cgatgaatgg ttaccgagca agtatctagt gacaaatgca ccggtgccca
     3961 aggttttttc agttccaact tcatttcttg gacaagagga taagactcaa aggtacgttc
     4021 caccagttta taggctaccg tttcaatcaa atcttcactc gtttcctgaa accaagtcgt
     4081 ccactgctga cacaattctc cgtaatggac agaggctgtt aaatccaagt ctgtagctgc
     4141 cttggtcata tcataggata ggatggctga aacgacaaat ttctgcccca attctttctc
     4201 actaggaaaa agaccatgat aggcaaacat ttccaaatcc ttaatctgca gttgatccat
     4261 aatgattcct ttctagaaaa atccgcttga agcggattct ttttatagcc ccattaaacg
     4321 ataagcttgg tcacggagat ccttatctgt ttcaaataga ccacgagcta ctgtcgtcaa
     4381 ggttgcagtg cctggttttc taacaccacg catactcata cacatatgtt ccgcctcaat
     4441 gacaacaaag gctcctttag cacctagata gtccatcaag gcatcggcca cttcgatatt
     4501 caaacgttct tgaatttgtg gttttttcga ataaacttca accgtacggg ctagcttaga
     4561 caagcctgcc acacgaccat ctggaatgta ggcaatgtgc gctctaccat aaaatggcaa
     4621 gaagtggtgt tcacacatgg tatggaaaaa gatatccttt tctaccacca tattatcgtc
     4681 aataatttca aaggattttg acaaatgttc ctctgctgtt tgaccaagac ctgaaaaaat
     4741 ctcttgatac atacgggcta cacgagcagg tgtttcctgc aagccctcgc gattaacgtt
     4801 ctctcctaca gcctcgataa tcatttttac agctgcttca atcttttgtg tatccattct
     4861 cgttcttcct ctccatcaga taggccctca cttggctcaa gaaatacaag gaacctgtga
     4921 caatcctaac tgtttgtttc tcttcttttt tatctgtcaa attctgatct agaaaatcat
     4981 gccaatcttg gtagttgaga tttctagact tagctgcctc tttcagcacg ctttcatccg
     5041 tcgcccgact atccgcaaaa tgtgttagag taagctcggt atctggcatg gctcccagca
     5101 agtccaacat atcctccaag gctttggttt tgatacaagt gaagaggatt tccttatgat
     5161 gatccgcaaa acgttcttgc aaggttacca acaaggcctt gatagcatgg ggattatggg
     5221 ctccatccaa aatcatcaag ggatctcttg acacgatttc caaacgccct ggccaacttg
     5281 tttcttccaa ggcttgacca agaaagtcat tgcttgctag ctctcgacca tcttcttgac
     5341 aaaaagtatc aagtaaagct atggccatcc cagcattctc tatttggtac aaaccaagca
     5401 ggctagtctg gaagcgacct tgtctgacag cacttgtata gtcaaagact tcccctgtca
     5461 ccacactttc ttgatgacga acctgataat ctgtcccgta ggcaagtctc ggcgcatctt
     5521 tcccttccgc aatgcggtca atcacagtca aggcttctgg agcaatacgc cctgttacca
     5581 agggcatccc ttgtttgata atacctgcct tctgctctgc tatgacctcc aaggtgtcac
     5641 caagtagggc cacatgatcc aagccaatag ttgtaattcc tgtcaaaatg ggctgacaga
     5701 cattggtact atccaaaagt ccacccatgc caacttccat gatggccaca tctacttgct
     5761 ctgaggcaaa gtagttatag gccagggctg tgataatctc aaactcagtt gtctcctgaa
     5821 aattggaagc cactcccccc tccagcaaag actgatagtc taccatgagg gcttctagtc
     5881 tagcctccgg gatagattcc ccattgatgc taatctggtc tgtataatga atgagatagg
     5941 gcgagctgaa aacaccaact ctcagcccta gtttttctag catatttttc aaaaaagcaa
     6001 tggtcgaacc cttgccattg gttcctccga tatggatgac tttgagtttg agatggggat
     6061 ttccgcgcaa agctaacagt tccaccattc gttccaagcc aaaatgcggt tgatccgtcc
     6121 ggtagttagc aatccactga ttgttttcaa tttctttcat cttatttata ttgttttaaa
     6181 tctaaatttt ccgcttcatc agccaatcga atggctgatg caatttcaac tgccatcttg
     6241 tgactggcta cgtcatgcac gcgcaccact tctacaccct gtcttgcagc gatactggtt
     6301 acatgagccg aagccgtatc tcgattgcgg aaaccaagtt ctgtctcagg attgacttca
     6361 aaaccatttt cttcaagaat attgatgaca aaccgcttac gcgacactcc aagaaagatt
     6421 ggatagccct tctgatgtag tttatccaaa tcccgtaaaa gaagcaaatt ttctttcttg
     6481 gtcagaccaa agccaattcc tagatccaac aggatatttt cttgtgcaat tccagcttga
     6541 tttgctctcg ctagtgcacg ttcaaagaaa gtctccatca agtcttcgat tggcaatttt
     6601 tcaaagttag ctaactcttc ctctgtaaaa gcttgtccaa aaccaaaatg aggaaagatg
     6661 agcgagctag gatgctgagg tcgagcaata actggattaa acatgatgac cactttcgca
     6721 ccagccttag caaccacctc cgccattttc tcatcaccca taagaccagt gatatcatta
     6781 actagattgg caccagcagc caaagcagct tctgctacct gactcttcca agtatcaata
     6841 gagatgagga catcactttc cttgcgaatc gctttaatca ctggaacaac acgctggatt
     6901 tcctcttcta tctcaacata gctatagcta cttcccggcc gagtcgattc tccgccgata
     6961 tctagcatac tagccccttc tgctatcaat ttacgagcct gctggagtgc ctgctcaaga
     7021 gcaaaaaatt gaccaccatc cgaaaaggaa tctggggtta cattgataat tccgcaaata
     7081 gctgtctttg catgatttac tttagttgac atatcggtca ctccctcaag gcttttcacc
     7141 atattatttc tctattttac cataaaaaga aaaagatgga cactattgca ttcatctttt
     7201 ttcccaatag aaacaagtaa gcaattgtca aaaatcttaa acagaaatcc ctaatgtccg
     7261 actcattatc accacaagtg ccaataaaca gaaggctacc ccattaacaa tcatgtgaag
     7321 caagatagac atttccaaac gttgagtctt gtaagctgtc caagatagaa cgattgacat
     7381 acctccataa atcaataaag aaggtaaatt acttggttga tgcaataaag caaagacaat
     7441 cgtaccaacc acaaacccca agttctcctt gcctcgaaaa atctttttag gaacaatccc
     7501 acggcacaag atttcctcac aaatcggagc aagcaaggct agcaagaaga agctagaaat
     7561 cagagaacta ttctgaacca tatcgttaat ctgagactgg ttagctgttg tcgtctcatt
     7621 tgacagttgc aacaagatag aaccaagtat atttgacccg acaataacta aataactcaa
     7681 gcccaaacgt gccaaatctt tagctctaaa aaaagaaaaa ttaaaactag ctaacttggt
     7741 tttacgagct cccataataa atagagccag aaccacaatt gaaaggccag caactatcag
     7801 ccctgactgt agcagggcta cttcttttaa agtcaaaata gaggtaacca ctaggggaac
     7861 ctgatataaa aggacggcaa gtaaaaagac caaaatccag cctgcacgat tcaacaattc
     7921 tttccacata tttttctctt ttatcaccta tctcctttgt attaaaaaag gggggaaatc
     7981 cccctgggtc attctattat aaggccatgc tgatgtagtt aaggataaac aaggcatcca
     8041 aaatccaaat catggcatga acatctttaa cttgaccttt gacaagctta gtcaaagtgt
     8101 aagtcaagaa accaactgca atcccttgag tgatagagta gctgaatccc ataaagatag
     8161 atgtgaagaa ggcaggaact gcttcagaca tatcatccca atggatattt ttcaagctac
     8221 caagcatcat aatcccaacg ataatcaaga ttggagctgt agccgctgtt ggtacgatcg
     8281 ctagaagcgg gctaaagaag cttgaaatcg caaaacagat agctacaacc aaggctgtca
     8341 aaccagtacg tccacctgca ccgataccag cagcagactc aacataagtc gttacgtttg
     8401 aagtacctgc aatggcaccg actgtcgttc caatcaaatc agagtaaaga gccttatcca
     8461 atttggctga ttggtgattt tcaccatttg tcgctacaat accaactttt tcacctgtac
     8521 cgatcaaggt accaattgtg tcaaaaatat ctgtcaatga gaaggcaaga atggccatca
     8581 gagtttcagg caagcgagct gtatctgaaa ccaaagctcc caaaccttct gaaccaagag
     8641 ctgcaccaaa gattgtcttc aaatcttcaa aagctgcacc aacatggtta ttagcaaaat
     8701 cgatactaga caaatcaacc aaaccaactg caatagcaag aacagttgtt gtcaagatag
     8761 agagaataat tcccccttta atccctttga tgacaaagaa gatagtaatg gcaagtcctg
     8821 caagagccac taaaacagct ggattattaa agctgaccaa tcctggaact gctgaagagt
     8881 ttgctgcaat cgttgcttga gctttgtcag ccccttctcc tacaacagta tagttgcctg
     8941 gatcaatcgt gaatttcaaa agtccagcat tcttaatccc tacataggca aggaagacac
     9001 cgataccagc tgaaatagct gagcgaagag cattgggaat cgattcaatg atcattttac
     9061 gaacatttgt caaggtaata atcaatgaaa taatcccaca gatgaagacc atagctaggg
     9121 cttcttgcca agaataacca agcccgaata caactgtaaa ggtaaagaag gcattgagtc
     9181 ccatacctgg cgcttgggca taaggtaagt tagcataaaa agccatcatc aaggtacccg
     9241 ctactgcacc aataatcgtc gctaggaaga cgccctgagc aggcattcct gtttgtgaaa
     9301 gtatttgtgg gtttacaaag agaatatagc tcattgcaaa gaaagttgtt aaaccagcga
     9361 gaacctctgt acgaacgtct gtaccgttct cttttagttt aaataatttg tccatcatca
     9421 atctcctttt aatttttacg aacaataata gaattatatc atttatcatt cactttttca
     9481 atgtctttgt ttgatttgtt taagaaatta atccaatttc attttttgtt tggaatctgc
     9541 ttttctgcct gctttctgtt ataatagaag acatcttatc tgaaaacaca ctagaaaggt
     9601 ccatatgacg aaaaaaatta ttgcagttga cctggatgga accctgctca actcagacag
     9661 tcaaatttct gactttacca aaaaaaccat caaaaaagtt gctgaaaaag gccatcaggt
     9721 tattattacg acaggtcgcc cttaccgtat gtcaaaagat ttttaccgtg aactgggctt
     9781 agacactcct atgattaact tcaacggatc ccttactcat ttaccagacc aagtttggga
     9841 ttttgaaaag tgtttgactg tagacaaaaa atatctgcta gatatggttc aacgttcaga
     9901 ggacattcaa gccgatttta tcgctggaga atatcgtaaa aaattctaca ttacaaatcc
     9961 caatgaagaa attgccaatc ccaaactatt tggtgtagaa gctttccagc ctgaagatca
    10021 attccagcct gaattggtga ccaaggaccc taactgtatc ctcttgcaga ccagagccag
    10081 tgacaaatat gccttggcaa aagacatgaa cgccttctac cagcatcaac tttctatcaa
    10141 tacctgggga ggtccgctca atatccttga atgtacccca aaaggtgtca acaaggcctt
    10201 tgctttggac tacttgctca agataatgaa tcgtgacaaa aaagatttga ttgcctttgg
    10261 agatgaacac aatgataccg aaatgctcgc ttttgctggg aagggttatg ccatgaaaaa
    10321 tgccaatcca gagctactcc cttatgcaga tgagcaaatt tcccttacca acgaccaaga
    10381 tggggttgcc aaaaccctac aagacttatt cttataacct atactgatac tcaatgagaa
    10441 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactgcttt gaggttgtag
    10501 atgaagctga cgtggtttga agagattttc gaagagtatg accagcttgc aagctggtct
    10561 ttgtgctctt ttcacagtct catcaaccag ttaatcgatt gttctacttt ttgtctccaa
    10621 aaccttggaa aaggcttcct tttgtgatat acttcacata taaatacaag agagctcgtc
    10681 acactcgact ctgttgacaa aaaatcaatc cagtcccgtt gtccctgttc tcggtcaata
    10741 tcaaggagga tagctatgaa agctgttgtt gtaaatccag aaagcactgg tgttgctatt
    10801 gaagaaaaag tactccgtcc acttgaaact ggggaagcac ttgtagaagt tgaatactgt
    10861 ggcgtttgcc acaccgacct ccacgttgct catggtgact ttggtcaagt cccaggacgt
    10921 gttcttgggc acgaaggtat cggtatcgtt aaagagattg ctccagatgt gaaaagcctt
    10981 aaagtcggtg accgcgtcag cgttgcttgg ttctttgaag gatgtggcac ttgcgaatac
    11041 tgtacaactg gtcgcgaaac cctttgccgt acagtgaaaa atgctggcta ctcagtagac
    11101 ggtggtatgg ctgaacaatg tatcgcaact gctgactatg ctgtcaaagt tcctgacgga
    11161 cttgatccag cccaagcttc ttctatcaca tgtgctggag taacaaccta taaagctatc
    11221 aaagaagcaa aagttgaacc aggccaatgg gttgttcttt acggtgctgg tggacttggt
    11281 aacctcgctg ttcaatacgc taaaaaagta ttcaatgctc atgttatcgc agtcgatatc
    11341 aacaatgaca aacttaccct tgcaaaagaa gtaggcgctg acattgtgat taacggcctc
    11401 gaagttgaag atgtagctgg actcattaaa gaaaaaactg atggaggagc tcattcagct
    11461 gtcgtaactg ctgtgtctaa agttgccttc aaccaggctg ttgactccat tcgtgctggt
    11521 ggtcgcgtcg tcgctgttgg tcttccttct gaaatgatgg aactcagcat cgttaaaaca
    11581 gtcctcgatg gaatccaagt catcggttct cttgtcggaa ctcgtaaaga cttagaagaa
    11641 gccttccaat ttggtgcaga aggtctggta gtcccagttg ttcaaaaacg tccagtagaa
    11701 gatgctgttg ccattttcga cgaaatggaa aaaggccaaa tccaaggacg tatggtactc
    11761 gacttcaccc actaatttaa tagaaaccta ttttaaaagt tggaagcttc ttccaacttt
    11821 tttatattaa attttaaata agaattcaag
//
LOCUS       DAVLTM010000055        11825 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLTM010000055 DAVLTM010000000
VERSION     DAVLTM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.056"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(128..1759)
                     /locus_tag="VYN16_001296"
     CDS             complement(128..1759)
                     /locus_tag="VYN16_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1403819.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFISAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAVL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2052..7031
                     /locus_tag="VYN16_001297"
     CDS             2052..7031
                     /locus_tag="VYN16_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1403820.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7121..8317
                     /locus_tag="VYN16_001298"
     CDS             7121..8317
                     /locus_tag="VYN16_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1403821.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADNVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8453..8980
                     /locus_tag="VYN16_001299"
     CDS             8453..8980
                     /locus_tag="VYN16_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1403822.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFCFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9057..9413
                     /gene="glnR"
                     /locus_tag="VYN16_001300"
     CDS             9057..9413
                     /gene="glnR"
                     /locus_tag="VYN16_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1403823.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9450..10796
                     /gene="glnA"
                     /locus_tag="VYN16_001301"
     CDS             9450..10796
                     /gene="glnA"
                     /locus_tag="VYN16_001301"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1403824.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10886..11005)
                     /locus_tag="VYN16_001302"
     CDS             complement(10886..11005)
                     /locus_tag="VYN16_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1403825.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11493..11825
                     /locus_tag="VYN16_001303"
                     /pseudo
     CDS             11493..11825
                     /locus_tag="VYN16_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3795 a         2164 c         2554 g         3312 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatat tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta agaagccatc caattatgat ggatagcttc
      121 ttggttatta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aagacggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 ctttcttagc atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga caactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtaga tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccagt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgatttttcg
     1381 taaaaaaaag acagacggca ctgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtgataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgt aaaccgtctc
     1681 ccatggtctt gatgctatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ctattttaaa ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat
     1861 tataattgat aagtgtatta tttttaagag tttttaacct tattttagat ttgtgcaaac
     1921 agttattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
     1981 gtttgataaa aaactacaga atataaagca ctttcttgaa aacaacaaaa tcaatctttt
     2041 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2101 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg
     2161 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2221 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatgct agagaaaaca
     2281 agctagaaaa ggcagaagga gtagcgatag catctgaaac tgcttcgcca gcaagcaatg
     2341 aagctgcaac tactgaaact gcagaagcag ctagcgcagc taaaccagag gaaaaagcaa
     2401 gtgaggtggt tgcagaaaca ccatctgcag aagcaaaacc taagtctgac aaggaaacag
     2461 aagcaaagcc cgaagcaact aaccaagggg atgagtctaa accagcagca gaagctaata
     2521 agactgaaaa agaagtccag ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa
     2581 aggaaatcaa atttaattct tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag
     2641 atgatgctat taaccgcgga tctgttgtcc tcgcttcacg tcggacaggt catttagtca
     2701 atgaaaaagc tagcaaggaa gcaaaagttc aagccttatc aaacaccaat tctaaagcaa
     2761 aagaccatgc ttctgttggt ggagaagagt tcaaggccta tgcttttgac tattggcaat
     2821 atctagattc aatggtcttc tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag
     2881 gtcaccgtaa cggggttcct gtatacggta cactcttctt caactggtct aatagtattg
     2941 cagatcaaga aagatttgct gaagctttga agcaagacgc agatggtagc ttcccaattg
     3001 cccgtaaatt ggtagacatg gccaagtatt atggctatga tggctatttc atcaaccaag
     3061 aaacaactgg agatttggtt aaacctcttg gagaaaagat gcgccagttt atgctctata
     3121 gcaaggaata tgctgctaag gtaaaccatc caatcaagta ttcttggtac gatgccatga
     3181 cctataacta tggacgttat catcaagatg gtttgggaga atacaactac caattcatgc
     3241 aaccagaagg agataaggtt ccggcagata acttctttgc taactttaac tgggataagg
     3301 ctaaaaatga ttacactatt gcaactgcca actggattgg tcgtaatcct tatgatgtat
     3361 ttgcaggttt ggaattgcaa cagggtggtt cctacaagac aaaggttaag tggaatgaca
     3421 ttttagacga aaatgggaaa ttgcgccttt ctcttggttt atttgcccca gataccatta
     3481 caagtttagg aaaaactggt gaagattatc ataaaaatga agatatcttc tttacaggtt
     3541 atcaaggaga ccctactggc caaaaaccag gtgacaaaga ttggtatggt attgctaacc
     3601 tagttgcgga ccgtacgcca gcggtaggta atacttttac tacttctttt aatacaggtc
     3661 atggtaaaaa atggttcgta gatggtaagg tttctaagga ttctgagtgg aattatcgtt
     3721 cagtatcagg tgttcttcca acatggcgct ggtggcagac ttcaacaggg gaaaaacttc
     3781 gtgcagaata tgattttaca gatgcctata atggcggaaa ttcccttaaa ttctctggtg
     3841 atgtagccgg taagacagat caggatgtga gactttattc tactaagtta gaagtaactg
     3901 agaagaccaa acttcgtgtt gcccacaagg gaggaaaagg ttctaaagtt tatatggcat
     3961 tctctacaac tccagactac aaattcgatg atgcagatgc atggaaagag ctaacccttt
     4021 ctgacaactg gacaaatgaa gaatttgatc tcagctcact agcaggtaaa accatctatg
     4081 cagtcaaact atttttcgag catgaaggtg ctgtaaaaga ttatcagttt aacctaggac
     4141 aattaactat ctcggacaat caccaagagc cacaatcgcc gacaagcttt tctgtagtga
     4201 aacaatctct taaaaatgcc caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg
     4261 atgcagattt ctatgaagtt tatgaaaaag atggagacag ctggaaatta ctaactggct
     4321 catcttctac aactatttat ctaccaaaag ttagccgctc agcaagtgct cagggtacaa
     4381 ctcaagaact gaaggttgta gcagtcggta aaaatggagt tcgttcagaa gctgcaacca
     4441 caacctttga ttggggtatg actgtaaaag ataccagcct accaaaacca ctagctgaaa
     4501 atatcgttcc aggtgcaaca gttattgata gtactttccc taagactgaa ggtggagaag
     4561 gtattgaagg tatgttgaac ggtaccatta ctagcttgtc agataaatgg tcttcagctc
     4621 agttgagtgg tagtgtggat attcgtttga ccaagccacg taccgttgtt agatgggtca
     4681 tggatcatgc aggggctggt ggtgagtctg ttaacgatgg cttgatgaac actaaagact
     4741 ttgaccttta ttataaagat gcagatggtg agtggaagct agctaaggaa gtccgtggta
     4801 acaaagcaca cgtgacagat atcactcttg ataaaccaat cactgctcaa gactggcgct
     4861 tgaatgttgt cacttctgac aatggaactc catggaaggc tattcgtatc tataactgga
     4921 aaatgtatga aaagcttgat actgagagtg tcaatattcc gatggccaag gctgcagccc
     4981 gttctctagg caataacaag gtacaagttg gctttgcaga tgtaccggct ggagcaacta
     5041 ttaccgttta tgataatcca aattctcaaa ctccgctcgc aaccttgaag agcgaagttg
     5101 gaggagacct agcaagtgca ccattggatt tgacaaatca atctggtctt ctttattatc
     5161 gtacccagtt gccaggcaag gaaattagta atgtcctagc agtttccgtt ccaaaagatg
     5221 acagaagaat caagtcagtc agcctagaaa caggacctaa gaaaacaagc tacgccgaag
     5281 gggaggattt ggaccttaga ggtggtgttc ttcgagttca gtatgaagga ggaactgagg
     5341 acgaactcat tcgcctaact cacgcaggtg tatcagtatc aggttttgat acgcatcata
     5401 agggagaaca gaatcttact ctccaatatt tgggacaacc agtaaatgct aatttgtcag
     5461 tgactgtcac tggccaagat gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc
     5521 aggaaccgaa aaaagattac ctagttggtg atagcttaga cttgtctgaa ggacgctttg
     5581 cagtggctta tagcaatgac accatggaag aacattcctt tactgatgag ggagttgaaa
     5641 tttctggtta cgatgctcaa aagactggtc gtcaaacctt gacgcttcat taccaaggcc
     5701 atgaagttag ctttgatgtt ttggtatctc caaaagcagc attgaacgat gagtacctca
     5761 aacaaaaatt agcagaagtt gaagctgcta agaacaaggt ggtctataac tttgcttcat
     5821 cagaagtaaa agaagccttc ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc
     5881 atgaaactag cacccaagat caagtcaatg accgacttaa taaattgaca gaagctcata
     5941 aagctctgaa tggtcaagag aaatttaagg aagaaaagac agagcttgat cgcttaacag
     6001 gtgaggttca agaactcttg gctgccaaac caaaccatcc ttcaggttct gccctagctc
     6061 cgcttcttga gaaaaacaag gccttggttg aaaaagtaga tttgagtcca gaagagcttg
     6121 caacagcgaa acagagtcta aaagatctgg ttgctttatt gaaagaagac aagccagcag
     6181 tcttttctga tagtaaaaca ggtgttgaag tacacttctc aaataaagag aagactgtca
     6241 tcaagggttt gaaagtagag cgtgttcaag caagtgctga agagaagaaa tactttgctg
     6301 gagaagatgc tcatgtcttt gaaatagaag gtttggatga aaaaggtcaa gatgttgatc
     6361 tctcttacgc ttctattgtg aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat
     6421 ttttcttacc tgaaggcaaa gaggcagtag aattggcttt tgaacaaacg gatagtcatg
     6481 ttatctttac agcaccacac tttactcatt atgcctttgt ttatgaatct gctgaaaaac
     6541 cacaacctgc taaaccagca ccacaaaaca aagtccttcc aaaacctact tatcaaccgg
     6601 cttctgatca acaaaaggct cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc
     6661 gtcaagagca tgaaaatgct gagatgctag ttggggaaca acgagtcatc atacagggac
     6721 gagatggact gttaagacat gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt
     6781 caacagaagt catccaagaa gcgattccag aaattgttga aattggaaca aaagtaaaaa
     6841 cagtaccagc agtagtagct acacaggaaa aaccagctca aaatacagca gttaaatcag
     6901 aagaagcaag caaacaattg ccaaatacag gaacagctga tgctaatgaa gccctaatag
     6961 caggcttagc cagcctcggt cttgctagtt tagccttgac cttgagacgg aaaagagaag
     7021 ataaagatta aatatcgaaa aatcttgtga aatctttcct tatatttcca aagtgtgata
     7081 taatagtttt gaataaaata aataaaggag tttttgtaac atggcaaaac ttactgttaa
     7141 agacgttgac ttgaaaggta aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa
     7201 agatggcgta atcactaacg ataaccgtat cacagcagct cttccaacta ttaagtacat
     7261 catcgaacaa ggtggacgtg caattctttt ctctcacctt ggacgtgtga aagaagaagc
     7321 tgataaagct ggcaaatcac ttgctcctgt agcagctgac ttggcagcaa aacttggtca
     7381 agatgttgtt ttcccaggtg tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct
     7441 tgaagatgga caagttctct tggttgaaaa cactcgttac gaagatgttg acggcaagaa
     7501 agaatctaaa aacgatcctg aacttggtaa atactgggca tcacttggag atggtatctt
     7561 cgtaaacgat gcattcggta cagctcaccg tgcacacgca tctaacgttg gtatctcagc
     7621 aaacgttgaa aaagcagttg ctggtttcct tcttgaaaac gaaattgcct acatccaaga
     7681 agcagttgaa actccagaac gtccattcgt ggctatcctt ggtggttcaa aagtttcaga
     7741 caagatcggt gttatcgaaa acttgcttga aaaagctgat aacgtcctta tcggtggtgg
     7801 gatgacttac acattctaca aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga
     7861 agacaaattg gatgttgcga aagctcttct tgaaaaagca aatggtaaat tgatcttgcc
     7921 agttgactca aaagaagcta acgcatttgc tggttacact gaagtgcgtg acactgaagg
     7981 tgaagcagtt tctgaaggct tccttggtct tgacatcggt ccaaaatcta tcgccaaatt
     8041 tgacgaagct ttgactggtg ccaaaacagt tgtatggaac ggacctatgg gtgtatttga
     8101 aaacccagat ttccaagctg gtacaatcgg tgtgatggac gctatcgtga aacaaccagg
     8161 agttaaatca atcatcggtg gtggtgactc agctgccgca gcgattaacc ttggccgtgc
     8221 agacaagttc tcatggatta gtacgggtgg tggagcatca atggaacttc ttgaaggtaa
     8281 ggttcttcca ggacttgcag ccttgacaga aaaataagat tttataaata aatcaaagaa
     8341 gagagggatg aaagttcctc ttttcttttg cttaaaataa aaacgcttcc tctcaactat
     8401 tactcataaa aatcaccgat ttatgataaa atggaaatag aaagttgaga ttatgagtta
     8461 ttttaaaaaa tataaattcg ataaatccca gttcaaactt ggtatgcgaa cctttaaaac
     8521 aggtattgct gtttttctag ttctcttgat tttttgcttt tttggctgga aaggtcttca
     8581 aattggtgct ttgacagccg tttttagcct gagggagagt tttgatgaga gtgttcattt
     8641 tgggacttcg cgtattctag gaaatagtat cggtggactc tatgccttgg tcttcttctt
     8701 attaaatacc tttttccacg aagccttttg ggtgaccttg gtagttgttc caatctgcac
     8761 catgttaacc attatgacaa atgtagcaat gaataacaaa gcaggggtta ttggtggtgt
     8821 agcagctatg ttaatcatta ccctatcaat tccaagtggt gagacaattt cgtacgtgtt
     8881 tgtgcgtgta tcagaaacgt ttatgggagt ttttgtcgca attatcgtaa attacgatat
     8941 tgatcgtatt cgtctctttt tagagaaaaa agaaaaataa tgttacattt tataacatta
     9001 tcaattgacg tttgtctttt tttagactat aatagacaga aagaaggaaa ttgtaaatga
     9061 aggaaaaaga atttcgccga aatatggctg tttttcctat cggcagtgtt atgaagttga
     9121 ccgatctatc ggcgcgtcag attcgttatt atgaagatca agagttgatc aagcccgatc
     9181 gaaacgaagg aaatcgtcgc atgtattcct tgaatgacat ggatcgtctg cttgaaatca
     9241 aagattatat ctctgaaggt tataatatcg ctgccattaa gaaaaaatat gctgaacgtg
     9301 aagcgaaatc caagaaagcg gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac
     9361 tcctccaaca ggggcgtttt gcttcagtac agtcaccttt tggtcgcggt taggcaaccg
     9421 caagtagtca tagattaagg agaaaactta tgccaatcac agctgcagat attcgtcgtg
     9481 aagtcaagga aaaaaatgtt acctttattc gtcttatgtt ctcagatatt ttgggaacca
     9541 tgaaaaacgt cgaaattcct gctacagatg aacagttaga taaggtcttg tcgaacaagg
     9601 ttatgtttga tggatcttct attgaaggtt ttgtacgtat caatgagtcg gatatgtact
     9661 tgtacccgga cttggataca tggacagtct tcccttgggg agatgaaaat ggaagtgttg
     9721 caggtctgat ctgtgatgtt tatacaacag aaggtgaacc atttgcgggt gaccctcgtg
     9781 gtaatttgaa acgagctctt cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg
     9841 gtccagagcc agaattcttc ctatttaagt tggatgaaaa tggggaccca acacttgaag
     9901 tgaatgacaa gggtggctac tttgatttgg cacctactga ccttgcggac aacacacgtc
     9961 gtgagattgt gaatgtcttg accaaaatgg gatttgaagt agaagcgagt caccacgagg
    10021 ttgcggttgg acagcatgag attgacttta agtacgatga agttctccgt gcttgtgata
    10081 agattcaaat ctttaaactt gttgttaaaa ccattgctcg caaacacgga ctttacgcaa
    10141 catttatggc gaagccaaaa tttggtattg ctggctcagg tatgcactgt aatatgtcct
    10201 tgtttgatgc agaaggaaat aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt
    10261 tgtcagaaac agcttaccat ttcctaggcg gtttgatcaa gcatgcttac aactatactg
    10321 ccatcatgaa cccaacagtt aactcataca aacgtttggt tccaggttat gaagcgcctg
    10381 tttacattgc ttgggctggt cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg
    10441 gtatgggaac tcgtcttgag ttgcgttcag tggatccaat ggcgaaccct tacattgcta
    10501 tggctgttct tttggaagtt ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc
    10561 ctatcgaaga aaatatctac atcatgacag cagaagagcg caaggaagct ggtattacag
    10621 accttccatc aactcttcac aacgctttga aagctttgac agaagatgaa gtggttaaag
    10681 ctgctctcgg agatcacatc tacactagct tccttgaagc caaacgaatc gaatgggcaa
    10741 gttatgcaac cttcgtttca caatgggaaa ttgataatta tttagacctt tactaatata
    10801 gaagaaagat tgcctgtggg taaacagtca cacagcagtc taagccatca aatacctata
    10861 acccttaaaa tagcgatttc ggtcgctatt tatttttcga aaaatcctct tgactctttt
    10921 gcgaattaga tttatcatca gacgtgtaga agaaaatcaa ggagtcacta tggaaaaatt
    10981 caatttcaaa aacaatatcg ggcaagaaaa caagttactt caaatcgaaa tctacaagtt
    11041 tactaacttt tgcaagcttc aaaattacac atctgtaaat attttttcga aagatatatt
    11101 tgaagccata gtaaattgag actagaatag tacactacat ctgctaaaaa tttctagaaa
    11161 ttaatttaac tttcctaatc gatttgttta catcttattt caatctacta tattatctca
    11221 ataaatgatg atggcgactg ttttgtttgt gcttgtcgat ttttgaatcc tgatcgagcg
    11281 gaagaatatt tctgccgagg ttgtcaaggg gaaaatcctg aagatattga attttatgat
    11341 gaacaattac aggcagaaaa ggtggaggct ttgaatattc gattggaagt aaaaaattga
    11401 attatctaca atctcatcaa caataaacct atttcaaccc cattatctat aggcgtattt
    11461 ttacgccttt ttatatactt gaagcacata agaaacatta tgggaagttt gctgatggaa
    11521 gcactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggataa
    11581 aatcaattta ttggaatttt gggcaaaata agccagagaa atcctttagg tatatagata
    11641 cgtctagtat tgatagaaaa aagaacataa tcaactacaa aaatctacaa tatctttcac
    11701 ctgaacaagc gccttcccgt gctagaaaat tagtttcgca gaatagtgtc ttattttcaa
    11761 cagttagacc atatctaaaa aatattgctg tagttagaga acttaaagag tatttgatag
    11821 ctagt
//
LOCUS       DAVLTM010000056        11652 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLTM010000056 DAVLTM010000000
VERSION     DAVLTM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.057"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            94..747
                     /locus_tag="VYN16_001304"
     CDS             94..747
                     /locus_tag="VYN16_001304"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1403826.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            757..1221
                     /locus_tag="VYN16_001305"
     CDS             757..1221
                     /locus_tag="VYN16_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1403827.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1234..2502
                     /locus_tag="VYN16_001306"
     CDS             1234..2502
                     /locus_tag="VYN16_001306"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1403828.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKVPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2516..2776
                     /locus_tag="VYN16_001307"
     CDS             2516..2776
                     /locus_tag="VYN16_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403829.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2787..4148
                     /gene="pdrM"
                     /locus_tag="VYN16_001308"
     CDS             2787..4148
                     /gene="pdrM"
                     /locus_tag="VYN16_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1403830.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4302..5270
                     /locus_tag="VYN16_001309"
     CDS             4302..5270
                     /locus_tag="VYN16_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1403831.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5286..6278
                     /locus_tag="VYN16_001310"
     CDS             5286..6278
                     /locus_tag="VYN16_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1403832.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFDPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKNAIRKTYNKE"
     gene            6496..7539
                     /locus_tag="VYN16_001311"
     CDS             6496..7539
                     /locus_tag="VYN16_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1403833.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDIIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7603..9288
                     /gene="lpdA"
                     /locus_tag="VYN16_001312"
     CDS             7603..9288
                     /gene="lpdA"
                     /locus_tag="VYN16_001312"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1403834.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EENGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVSGMESSLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVAAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9352..10341
                     /locus_tag="VYN16_001313"
     CDS             9352..10341
                     /locus_tag="VYN16_001313"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1403835.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDALRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10414..11484)
                     /gene="xerS"
                     /locus_tag="VYN16_001314"
     CDS             complement(10414..11484)
                     /gene="xerS"
                     /locus_tag="VYN16_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1403836.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3449 a         2187 c         2629 g         3387 t
ORIGIN      
        1 gtcgatttat taaatcttta gataaattgt aaaaagtggt ttccatagcc actttttgct
       61 ataatagagg cagtaaaaat gaaggagtca gctatggaac actcgtcttg gcatgctttg
      121 attaaggcgc aattacctga gggttatttc gggaaaatca atcagtttat ggagcaggtc
      181 tattctcagg ggattattta tccacccaag gaaaaggttt ttcaggctct cttgacaaca
      241 ctgcttgaag aagttaaggt ggtaattcta gggcaagacc cctatcacgg accaggtcaa
      301 gcgcagggct tgagtttttc tgtacctgac tctatcccag ctccaccatc cttgcaaaat
      361 atcttgaaag aattgtcaga tgatatcgga gttaagaaat ctcatgattt gacagcttgg
      421 gctgagcaag gagtcttgct tcttaatgct tgtttgactg ttcctgctgg acaggccaat
      481 ggtcatgctg gtcaaatctg ggagcctttt actgatgctg tgattcaggt ggtcaatcat
      541 ctagatagac cagtcgtttt tgtactctgg ggagcttatg cacgtaagaa gaaggcctta
      601 gttaccaatc ctcatcactt gattatcgaa tcagcccatc caagtccttt gtcggtttat
      661 agaggatttt ggggttccaa gcccttttcc aaggccaatg cattcttaaa agagacagga
      721 caagagccaa tcgattggct tagataagga gaaaatatgc ctcagttagc gacgatttgc
      781 tacattgata atgggaaaga actgctcatg ctccatcgta ataagaaacc caatgatgtc
      841 catgaaggga aatggattgg tgtgggtggt aagctagaga gaggagagac gccccaggaa
      901 tgcgcggtgc gtgaaatcct tgaagaaaca gggctcaaag ccaagccagt tctaaaaggt
      961 gtcatcactt ttcctgaatt tacaccagat ttagactggt acacctatgt ttttaaggtg
     1021 acggagtttg agggcgactt gattgactgc aatgagggga cgctagaatg ggttccctat
     1081 gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca cctttgttga gtggctttta
     1141 gaggataaac ccttcttttc agccaagttt gtttatgatg gggataaatt gttggatacc
     1201 caagttgatt tctatgaata aaggagaaag cagatgctac taatcaaaaa tggtcgtgta
     1261 atggatccta agtctggttt ggatcaagtt tgtgatgtct tagttcaaga tgggaaaatt
     1321 atcaaaattg cgcctgagat cacggaagaa ggagcagaaa cgattgatgc tactggtctt
     1381 gtggttgctc ctggtttggt cgatattcat gtgcatttcc gtgaacctgg tcaaacacat
     1441 aaagaagaca ttcatactgg tgccctagca gccgctgcag gtggttttac tactgtcgtc
     1501 atgatggcta ataccagtcc aaccatttca gacgtggaga ctttgcaagc agtcctccag
     1561 tcagctgcca aagagaagat taatgtcaag acagttgcga ccattactaa aaactttaat
     1621 ggtaaaaact tgactgactt taaggcactt ttagaagctg gtgcggttgg tttctctgat
     1681 gacggtattc cgcttgagag cagtaagatt gtcaaggaag ccatggagga agccaaaaaa
     1741 ctcaatacct ttatcagcct tcatgaggaa gatccaggtt tgaacggtgt tcttggcttt
     1801 aatgaaaata ttgctagaga acatttccat atctgcggtg ctactggggt ggctgagtac
     1861 gctatgatgg cgcgtgatgt catgattgcc tatgcaacta aagcccatgt tcacatccag
     1921 catttgtcta aggaagaaag tgttaaagta gtggagtttg ctcaggggtt aggtgcagaa
     1981 gtcacagcag aagtagcgcc acagcatttc tctaagacag aagcacttct tttaacacaa
     2041 ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat cagaccgtcg tgccgttatc
     2101 gaaggtctca aatcaggtgt catcacagtt attgcgactg accacgcgcc tcatcatgta
     2161 gatgaaaaaa atgttgagga tattaccaaa gtaccatctg gtatgactgg cttagaaaca
     2221 tccctgtctc tcggcttgac ctatttagta gaagctggtg agttgagctt gatggaatta
     2281 cttgaaaaaa tgacatacaa cccagccaag ctttacaact ttgaagcagg ttacttggct
     2341 gagaatggtc cagcagatat cactattttt gatgccaagg ctgaccgcct tgtggactcc
     2401 cattttgctt ccaaagcagc taattcacca ttcatcggtg aaaccttaaa agggcaggtt
     2461 aaatatacca tctgtaaggg acaaatcgtc tatcaagctt gataggagtt agaatatgaa
     2521 ttattttcga aaacgtaggg agagacaagc gaaatctaat agtggaattt actgtcccgc
     2581 agctaatcgt tccaaaattc aatatgaaac taaagaaaaa gctgaccatg cggttcaata
     2641 tgataacgga ggtttagtta gaagctatta ttgtcgtaca tgtgcgtgtt ggcatactac
     2701 ttcaaaatct aataaacctc cgctaagttt gaaaaactat ggtctgaaac tctttggttt
     2761 agataaatca gaatagaaag agaagtatgt ataagacaaa gtgtttacga gagaagttag
     2821 tattattttt aaaaattttc ttcccaatcc tgatctacca atttgccaat tattctgcct
     2881 cttttgttga tactgcaatg acaggtcaat acaacactat ggacttggct ggtgtatcta
     2941 tggcaaccag tatctggaat cctttcttta catttctaac agggattgtg tcagccttgg
     3001 tgcctatcat tggtcaccat cttggtcgag gcaaaaagga agaagttgcg tctgattttt
     3061 accaatttat ttatttggcc ttgggcctat ctgtggtctt gctggggatg gtacttttct
     3121 tggcaccaac aatcttgaat catattgggt tagaagcagc agtagcggca gtagcggttc
     3181 gctatctttg gtttttatct atcgggatta tccccttgtt gctctttagc gtcattcgtt
     3241 ccttgctgga ttcgctgggc ttgaccaaac tgtccatgta cctcatgctt ttgttactcc
     3301 ctctcaatag cggatttaac tatctcttga tttacggtgc ctttggtgtt ccagaactgg
     3361 gaggggctgg tgctggttta ggaacatcct tggcctactg ggtcttgctt gggatttctg
     3421 ttctggtttt atttaaacag gagaagctca aagccttaca ccttgagaaa cgaattccac
     3481 ttaatatgga taaaattaag gaaggagttc gtttaggtct gcctattggg ggaactgtct
     3541 tcgcggaagt ggctgtcttt tcagtggttg gcttgattat ggctaagttt tcgtccttga
     3601 ttatagctag tcaccagtca gctatgaact tttcaagtct tatgtacgcc tttcctatga
     3661 gtatctcatc ggctatggct attgtcgttt cctatgaagt gggagccaag cgatttgatg
     3721 atgcgaaaac ctatattggt ctaggaagat ggactgccct catttttgcg gccttcacct
     3781 taaccttcct ttacattttt aggggaaatg tggccagtct ttatggtaac gacccaaaat
     3841 ttatcgattt gacagcgcgt tttttaactt atagtctttt cttccagtta gcagatacct
     3901 ttgcggcgcc gcttcaggga attttgcggg ggtataagga tacagttatt cctttttacc
     3961 ttggtttgct tggttattgg ggcgtagcaa tccctgtggc tacgctattt gattccctaa
     4021 cagattttgg agcttattct tactggatcg gcttgattat tagtttgatt gtgagtgggg
     4081 cgctctaccg ttggcgttta actgtgatta tgaagagatt tgaatcttta gcaaaatcca
     4141 aacgctaaaa agtttgtgaa aaaatatcct tgacaagaaa atcccttgtt ttcacggggt
     4201 tttctttttt attttgtgaa atttctttta gaaaaacttt gacagtattt gccaaaagcg
     4261 gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa gatgtcaact ttagataaaa
     4321 atcttttgct agaaatgttc cgtaagatgg aagaaatccg tcgcatggac ttaaaaattg
     4381 cacaattagt aaagaaaggg aaagtgccag gaatgacgca cttttctgtt ggtgaagagg
     4441 cagctaacgt gggggctatg ttagctctca atccagatga tctgattacc tcaaaccacc
     4501 gtggacacgg gcaagctatt gctaaaggga ttgacctcaa cggaatgatg gctgaaatcc
     4561 ttggtaaata cactggaacc tgtaaaggga aaggtggatc tatgcatatc gctgaccttg
     4621 atgctgggaa ccttggtgcc aatggtatcg taggtggtgg tatggggatc gctgtcggtg
     4681 cagccctcag tcagcaaatg caaaataccg gtaaaatcgt tgtctgcttc tttggagatg
     4741 gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat ggcttctatc tggaacctgc
     4801 cagtcatttt ctattgcatt aacaacggtt acggtatctc tgcggatatc aagaaaatga
     4861 ccaatataga acatatccat caacgtagcg ccgcttatgg aattcctgga atgttcatcg
     4921 aagatggtaa caatgtcatc gatgtctatg aaggatttca gaaagctgtg gaccatgttc
     4981 gcagtggcaa tggtccagtc ttgattgaaa gtgtaactta tcgctggctc ggtcactcat
     5041 catctgaccc tggtaaatat cgtacgcgtg aagaagtgga attgtggaaa caaaaagatc
     5101 caatcgaaaa cctccgcaat taccttattg aaaataacat tgcaagtgcc gaagaattgg
     5161 aagaaatcca agcgcaagta aaggaagcag tagaagcttc tgttaaattt gcagaggaaa
     5221 gcccattccc accgcttgaa tcagcctttg aagatattta cgcagactaa ggagaaagag
     5281 ataaaatgga aacaaaaaca atgtccttcc gtgacaccat tatccttgct atgtctgagg
     5341 aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga cgtcggtgtc ttcggaggag
     5401 acttcggtac ttctgttgga atgcttgaag aatttgatcc agaacgtgtc cgtgactgtc
     5461 cgatttctga agctgccatc tctggagcag cagcaggagc agccatgaca ggacttcgtc
     5521 caatcgtcga tatgaccttc atggacttct ctgttattgc catggacaat atcgtcaacc
     5581 aagctgctaa aacacgttac atgtttggtg gtaaaggtca ggttccaatg actgttcgtt
     5641 gtgcagctgg taacggagtt ggctctgccg cccagcactc acaatctcta gagtcttggt
     5701 ttactcacat tccaggactt aaggttgtgg cacctggaac acctgcggac atgaaaggcc
     5761 tgcttaagtc ttctatccgt gataacaacc cagttattat acttgagtac aagtcagaat
     5821 ttaaccaaaa aggggaagtg ccagttgatc cagactacac aattccactt ggggttggtg
     5881 aaatcaaacg ccaaggaacg gatgtaacag ttgttactta tggaaaaatg cttcgccgtg
     5941 tggttcaagc tgctgaagaa ttagcagaag aaggaatttc agttgaaatt gttgacccac
     6001 gtacccttgt tccgcttgat aaggatatca tcataaactc agtgaagaag actggtaagg
     6061 ttgttctggt caacgatgcc cacaaaacaa gtggctatat cggtgaaatt tcagctatta
     6121 tttcagaatc agaagcattt gactatctag atgcaccaat tcgccgttgt gcaggagaag
     6181 atgtgccgat gccttatgca caaaacctag aaaatgcaat gattccaaca gttgaaagta
     6241 tcaaaaatgc aatccgaaaa acttataaca aagaatagag ttacataaga taagttatgt
     6301 aaataaaata aagctgacga gaaactttgt atcttttagg tgcagagctc ttcgtcatta
     6361 atatcttaga ttagagcacg ggctaaaagc tcggaaaaaa gataaatctc cttggcttca
     6421 tcgaagccat cgtcgatttc ctatttttca gtcgcttttg acgccctttg tatcataaat
     6481 agaattggag aggtcatggc tgatgacaag ctaagagcga ctcctgcagc tagaaagtta
     6541 gcggatgatt tagggataaa cctctacgac gtttctggct caggtgcaaa cggtcgtgtc
     6601 cacaaagaag acgtggaaac ttataaagac acaaacgtgg ttcgcatttc gccacttgca
     6661 aaacgaattg ccctcgaaca taacattgct tggcaggaaa tccaaggaac cggtcatcgt
     6721 ggtaaaatca tgaagaagga tgttttggcc ctgcttcctg aaaatatcga aaacgatagc
     6781 atcaaatctc ctgctcagat tgaaaaagtg gaagaagtcc ctgataacgt aacaccatat
     6841 ggtgaaatcg agcgtattcc aatgacacca atgcgtaagg ttattgccca acgcatggtt
     6901 gaatcttact tgactgcgcc aaccttcacc ctcaactatg aagttgatat gactgaaatg
     6961 ttggctcttc gtaagaaggt tcttgagcca atcatggaag caactgggaa gaagactact
     7021 gtaacagacc ttctttcgct tgcagttgtt aagactctta tgaaacaccc atacatcaat
     7081 gcttcattga cagaagatgg caagactatt atcactcaca actatgtcaa tcttgccatg
     7141 gcagttggga tggataatgg attgatgaca cctgttgttt acaatgctga gaagatgagt
     7201 ctttcagaac tggttgtagc ctttaaggat attattggcc gtaccttgga tggtaaattg
     7261 gctccaagtg agttacaaaa ttcaacattc acaatcagta atttgggaat gtttggtgtt
     7321 cagtcctttg gcccgattat taaccaaccc aactcagcta tccttggtgt cagttcgaca
     7381 atcgagaagc cagttgtcgt caatggtgaa attgtgattc gcccaatcat gagtttagga
     7441 ttaaccattg accaccgtgt cgtagatggt atggctggtg ctaagtttat gaaggacttg
     7501 aaagagttga ttgaaacgcc aatctcaatg ttaatttaag agcaagagta ttcattttta
     7561 agatatagat aaaggaagga agacatggcc ttagaagtaa ttatgccaaa agccggcgtg
     7621 gatatgacag aaggacaaat cgtccaatgg aataaaaaag tcggagaatt tgtaaaagaa
     7681 ggagaaatcc ttttggaaat catgactgat aaagtcagca tggaattgga agccgaagaa
     7741 aatggatact tgattgccat tctcaaagga gatggtgaaa ctgtccctgt aacggaagtt
     7801 atcggttacc ttggtgaaga aagggaaaac atcccaacag ctggagcagc atcgccagaa
     7861 gctagccctg tacctgtagc aagtacctca aacgatgatg gtaagagcga tgatgctttt
     7921 gatatcgttg tgattggtgg aggtcctgct ggttatgttg cagccattaa agctgcccaa
     7981 ctcggcggta aggttgccct tgttgagaaa tctgaacttg gtggaacctg cttaaaccgt
     8041 ggatgtattc caaccaagac ctaccttcat aacgctgaaa ttattgaaaa tatcggtcat
     8101 gctgcaaatc gtggtatcgt gattgaaaat cctaacttca ctgttgatat ggaaaaactt
     8161 ttagaaacta aatctaaagt tgttaatact cttgttggtg gtgttgcagg acttcttcgt
     8221 agctacggag ttactgttca taaaggaatt ggtacaatca ctaaagacaa gaacgtcttg
     8281 gtaaatggtt ctgaattgct tgaaaccaag aaaatcattc ttgctggtgg ttcaaaagtc
     8341 agcaagatca acgtctctgg tatggaatct tcacttgtca tgactagtga tgacattctt
     8401 gaaatgaacg aagtgccaga aagccttgtt atcatcggtg gtggagttgt cggtatcgaa
     8461 ctcggtcagg ccttcatgac atttggttca aaagtgactg ttatcgaaat gatggaccgt
     8521 atagttccag ctatggatgc ggaagtttct aagaaccttc gcttgatcct tgagcgtaaa
     8581 ggaatgacca tcttgactgg tactaaactg caagaaatca ttgaggaaaa tggtcaactt
     8641 cgtatcaagg ttgaaggaaa agacgatatc atcgcaagca aagctcttct ttcaattggt
     8701 cgtatgccag accttgaagg tattggagag gttgagtttg aattggatcg tggttgtatc
     8761 aaggtcaacg aatacatgga aacttcagtt ccaggcattt atgcaccagg tgacatcaac
     8821 ggtactaaga tgttggctca cgcagctttc cgcatgggtg aagttgccgc tgaaaatgcc
     8881 cttaaaggaa atcatgcagt tgccaaattg aatttgactc ctgcagccat ctacactctc
     8941 cctgaagtag cagcagtagg tttgacagaa gaacaagccc gtgagaaata cgatgttgcc
     9001 atcggtaagt ttaactttgc tgctaacggt cgtgctattg catctgacgc agctcaaggt
     9061 ttcgtaaaag ttatcgctga taagaaatac ggagaaatcc ttggtgtaca cattattggt
     9121 cctgcagccg cagaattaat aaacgaagca tcaagcatca tcgaaatgga aatcactgtt
     9181 gaggaaatgc tgaagaccat ccacgggcac ccaacttact ctgaagtgat gtacgaagcg
     9241 tttgcagatg ttctaggaat ggccatccat tcacctaaga aaaaataaaa cgaagataga
     9301 ttagactgga aggttatttc cagtttctat catataaagt aaagggatat catgaaatac
     9361 attatcaatc attcaaatga cactgctttt aatattgcct tggaagaata cgcctttaaa
     9421 caccttttgg atgaggatca aatcttccta ctttggatta acaagccatc tatcattgtt
     9481 ggtcgtcacc agaacactat cgaagaaatc aaccgtgatt atgttcgcga aaatggtatt
     9541 gaggtagttc gccgtattag tggtggtgga gctgtttacc acgatttaaa taacctcaac
     9601 tacacgatca tctccaaaga agatgaaaat aaggcatttg acttcaagag cttctcaact
     9661 ccggttatca ataccctggc tcaactcggg gttaaagctg agttcacagg tcgtaatgac
     9721 cttgagattg atggtaagaa gttctgtggc aatgcccaag cctatatcaa cggccgtatc
     9781 atgcaccacg gttgcttgct ctttgacgtt gatttgtcag tcctcgcaaa tgcccttaag
     9841 gtttcaaaag ataaatttga atcaaaaggt gtgaaatccg tccgtgcccg tgtaactaat
     9901 attatcaatg aattaccaaa aaaaatcaca gtcgaaaaat tccgtgattt actcttggaa
     9961 tacatgaaaa aagagtaccc agagatgact gaatacgtct tttcagaaga agaattggcc
    10021 gaaatcaatc acatcaagga tactaagttt ggaacttggg actggaacta tggtaaatca
    10081 cctgaattta acgtccgtcg tggaaccaaa ttcactagtg gtaaggttga agtctttgct
    10141 aacgttactg aatcaaaaat ccaagacatc aagatttatg gtgacttctt tggtattgaa
    10201 gacgttgctg cagtagaaga tgcccttcgt ggggtgaaat acgaacgcga agatgttctt
    10261 aaggcgctaa aaaccattga tatcacacgc tacttcgctg gtattagccg tgaagaaatc
    10321 gctgaagcgg tagttggata aatcaaaaag aagttggcca actaaccaac ttcttttttt
    10381 gatatttatt tacataattt aaaatacgta aaattataaa ctatctagag cattcttttg
    10441 ttcatcatta acaatatggg tatagaggtc agtgacttgt gtgctagcat gtcctagttg
    10501 gtgactgact aaaacttgtg atttagttgc atcatagagc ctagttgcta gtgtatggcg
    10561 cagtttatgg ggtgttacac gcactttaaa atcctctgag tatttagcaa ccattttctc
    10621 aacgctagaa gcatcgatac gattaggaac acctctgtag agagttaaaa aaagggctgt
    10681 atctgttttt tccgttttat agcgttgatt ccgaatggcc agataattct ctaaataagg
    10741 tttagcaaaa gcagcgacat tgactgagtc acgtttgcca ccttttcgag taacatcaat
    10801 aaccatcatt tttagattga gatctcttag atctagatta acagcttcag ataagcgaac
    10861 accagatgcc aagagaaggg caataatggc taaatctcgt tctttatttt tgttgaatga
    10921 tgagagagct cgatttgaaa gctgttgtgg gtgctcttga tcgatataag ttagaaaacc
    10981 ttctgtttca tcacctagaa agagtttttg cttgatattt tcagctctgg cagcaagggt
    11041 ttctttcttt ttcttggttg aaactttttt cattacatta cgatagaaat aaggttcccc
    11101 ctgatcgttt tcaacctcct cggttagata tttgtaaaga ctagaaagtg ctgataaggt
    11161 tcgattgata gttgtctgtg aaacaccttg ttttgttgta ttagcattca gcaagggacg
    11221 ttcacgtaga taaaggataa aggattccat gtctttctta gacatatttt ccaaaactga
    11281 taaaggaata tcggatattt tatcagcgtt tgaaatacca gactccaaaa cccagctgaa
    11341 aaatcggtca tattccttaa ggtattcgta cagggttgta aaactgtagg gcacagccag
    11401 cttagattgg tagtattcca gaacatacca gggcatgagt tgttttagtt tgtcgattcg
    11461 ttccagtaaa atctcacgtt tcatgtattt tctccataag taagtatact agtagtatag
    11521 catagacttg tatatttttc aagaaaatta tagtttttct gaaagatgtt ataggagttt
    11581 ttcaaagtga taaaaagact tagacctaca aacaaaacag aataaggaaa ataataaaaa
    11641 tctcacccac ta
//
LOCUS       DAVLTM010000057        11579 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLTM010000057 DAVLTM010000000
VERSION     DAVLTM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.058"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            177..854
                     /locus_tag="VYN16_001315"
     CDS             177..854
                     /locus_tag="VYN16_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1403837.1"
                     /translation="MISKRLELVASFVPQGAILLDVGSDHAYLPIELVERGKIESAIA
                     GEVVEGPYQSAVKNVEAHGLEEKIQVRLANGLAAFEEEDRVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            841..1638
                     /locus_tag="VYN16_001316"
     CDS             841..1638
                     /locus_tag="VYN16_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1403838.1"
                     /translation="MLASEVTQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            1655..2758
                     /locus_tag="VYN16_001317"
     CDS             1655..2758
                     /locus_tag="VYN16_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1403839.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYAVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            2765..2911
                     /locus_tag="VYN16_001318"
     CDS             2765..2911
                     /locus_tag="VYN16_001318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403840.1"
                     /translation="MKIKEQTRKLAADYSKHCFEVVDRTDEVSNHTHGKATLTWFELD
                     FRRV"
     gene            3014..4033
                     /gene="galE"
                     /locus_tag="VYN16_001319"
     CDS             3014..4033
                     /gene="galE"
                     /locus_tag="VYN16_001319"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1403841.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLKIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4035..4988
                     /locus_tag="VYN16_001320"
     CDS             4035..4988
                     /locus_tag="VYN16_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1403842.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(5050..5256)
                     /locus_tag="VYN16_001321"
     CDS             complement(5050..5256)
                     /locus_tag="VYN16_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1403843.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5308..5784
                     /locus_tag="VYN16_001322"
     CDS             5308..5784
                     /locus_tag="VYN16_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1403844.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            5793..6464
                     /locus_tag="VYN16_001323"
     CDS             5793..6464
                     /locus_tag="VYN16_001323"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1403845.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYIDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            6595..6933
                     /locus_tag="VYN16_001324"
     CDS             6595..6933
                     /locus_tag="VYN16_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1403846.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            6936..7565
                     /locus_tag="VYN16_001325"
     CDS             6936..7565
                     /locus_tag="VYN16_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1403847.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            7805..8956
                     /locus_tag="VYN16_001326"
     CDS             7805..8956
                     /locus_tag="VYN16_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1403848.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            9274..10023
                     /gene="truA"
                     /locus_tag="VYN16_001327"
     CDS             9274..10023
                     /gene="truA"
                     /locus_tag="VYN16_001327"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1403849.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            10013..10786
                     /locus_tag="VYN16_001328"
     CDS             10013..10786
                     /locus_tag="VYN16_001328"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1403850.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            10764..11225
                     /locus_tag="VYN16_001329"
     CDS             10764..11225
                     /locus_tag="VYN16_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1403851.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNDWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            11500..>11579
                     /locus_tag="VYN16_001330"
     CDS             11500..>11579
                     /locus_tag="VYN16_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1403852.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSN"
BASE COUNT         3292 a         2176 c         2668 g         3443 t
ORIGIN      
        1 ctgaataaac tgtttatttt ttattttttg caaattgttt aaaatctttc aattggattc
       61 acttattact ttaggacatt gaaacttctt tcgccagagg tgcttatatt actgggaagt
      121 tgaagattga aatgtggtat aataagaggt aatagagttt tggaaagaga gagaagatga
      181 tttcaaagag attagagttg gtagcttcct ttgtgccgca gggagccatt ttactagatg
      241 tggggagtga ccatgcttat ttgcctatag agttagtcga aagaggaaaa atcgaaagtg
      301 ccattgcagg tgaagttgtg gagggacctt atcagtctgc tgtcaaaaat gttgaggccc
      361 acggcttgga agaaaaaatc caagtccgtt tagccaatgg tttggcagct tttgaagagg
      421 aagaccgagt gtctgtcatt accattgctg gcatgggtgg tcgtttgatt gctaggattt
      481 tagaagaagg tttggggaag ttagctaatg tagagcgttt gatcctccag cccaataatc
      541 gtgaagacga cttgcgtatc tggctacagg atcatggatt ccagattgta gcagaaagca
      601 tcttagaaga agctggaaag ttttatgaga ttttggtggt ggaagcagga caaatgaagc
      661 tatcagccag tgatgttcgc tttggtccct tcttgtccaa agaagtcagt ccagtatttg
      721 tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc cctcggacaa atcccagaaa
      781 aaaatctgga agaacgtcaa gttctagtag ataagattca agctatcaag gaggtgctcc
      841 atgttagcaa gtgaagtgac ccaagcttat gaagcctttt gccctcagga attttctatg
      901 gagggagaca gccgtggtct gcaaattggc actttagaca agggtatcca aagggtcatg
      961 gttgccctcg atattcgtga agagacagtg gcagaggcca ttgaaaaggg tgtggacttg
     1021 attatcgtca agcacgcgcc tatctttcgt cctatcaagg acttgcttgc cagccgtccg
     1081 caaaatcaga tttacatcga cctgattaag catgacatcg cagtttatgt cagccatacc
     1141 aatattgata tcgttgaaaa tgggctcaat gactggtttt gtcagatgct agaaatcgag
     1201 gagacgactt atctgcagga aacaggtcca gaacgtggaa ttggacgtat tgggaatatt
     1261 cagcctcaga cattttggga attggcccaa caggtcaagc aagtctttga cctagatagc
     1321 cttcgaatgg tgcattatca agagaatgat ttgcagaagc ctatttcaag agtagcaatt
     1381 tgtggtggaa gcgggcagtc tttctataag gatgctttgg caaagggggc agatgtctat
     1441 atcactggtg atatctacta ccatactgct caggatatgc tgtctgatgg cttgttagca
     1501 ttggatccag gtcactatat cgaagtgctt tttgtggaaa aaatcgcagc actccttact
     1561 caatggaaag agaaaaaagg ctgggaacta gagattttac ctagtcaagc atcgaccaat
     1621 cctttccgtc atatctagtt agaaagtgaa gacaatgaaa aaagttgcca ttattggagc
     1681 aggaattgtg ggagcaacag ctgcctacta cctctcgaga gaaagtgacc tagaggtgac
     1741 cgtttttgac catgagcaag gtcaagccac caaggccgca gcaggaatta tcagtccttg
     1801 gttttccaaa cgccgtaata aagcctggta caagatggcg cgcttggggg ctgattttta
     1861 tgtggattta ttagctgatt tagagaaatc aggacaaaaa atcgactttt accagcgttc
     1921 gggagtcttt ctcttgaaaa aggatgaatc caagttggaa gaactttatc aactggccct
     1981 ccagcgcaga gaagaatctc ccttgatagg gcaattagcc attctggacc aagcctcagc
     2041 taatgaatta ttccctggtt tgcagggatt tgaccgcctg ctctatgctt ctggtggagc
     2101 gagagtagat ggccaactct tggtaactcg tctgctagaa gccagtcatg tcaagctggt
     2161 caaagaaaaa gtgactctga caccgttagc atcaggctac cagattggtg aagaggagtt
     2221 tgagcaggtt attttggcga cgggagcttg gttgggggac atgttagagc ctttaggtta
     2281 tgctgtggat gtccgtcctc aaaaaggaca actacgagat tatcagctgg tccaagatat
     2341 ggaagcttac cctgttgtca tgccagaagg ggagtgggat ttgattcctt ttgcaggtgg
     2401 gaaattgtcc ttaggcgcta cccacgaaaa tgacatggga tttgatttga cggtagatga
     2461 aaccttgctc caacaaatgg aggaggccac cttgactcac tatctgattt tggctgaagc
     2521 tacttcaaaa tctgagcgtg ttggaatccg tgcctacacc agtgatttct ctcctttctt
     2581 tgggcaggtg cctgacttaa ctggtgtcta tgcagccagt ggactaggtt catcaggcct
     2641 cacaactggt cctattattg gttaccattt agctcaactg atccaagaca aggagttgac
     2701 cttggaccct gaaaactacc caattgaaaa ctatgtcaaa cgagtaaaaa gcgaataata
     2761 ctcaatgaaa atcaaagaac aaactaggaa actagccgcg gattactcaa agcactgctt
     2821 tgaggttgtg gatagaactg acgaagtcag taaccatacc catggcaagg cgacgttgac
     2881 gtggtttgaa ttggattttc gaagagtata agcattttac tgaaatttta tcaggtagtt
     2941 taggatggca aatgacattc cctgtcaaaa atggtaaaat aagaaaaaat aatctgagaa
     3001 tcgaggaaaa aagatgcaag aaaagatttt ggtaactggt ggtgccggtt ttattggaac
     3061 ccacactgtt attgaattga tccaagcagg ccatcaagtt gttgtggtgg ataatcttgt
     3121 caatagcaat cgtaagagtt tagaagttgt tgaaagaatc acaggagttg agattccttt
     3181 ctatgaggca gatattcgtg acacagatac cctcagagat attttcaagc aggaagaacc
     3241 gactggtatc attcactttg ctggtttgaa ggctgttggc gaatcaacac gtatccctct
     3301 tgcctactat gacaacaata tcgctggaac tgtcagcctt ttgaaagcca tggaagaaaa
     3361 caactgtaaa aacatcatct tcagttcttc tgcgacagtt tacggggatc cgcacacagt
     3421 gcccatcttg gaagatttcc cactttcagt gaccaaccca tacggtcgta ccaagctcat
     3481 gctagaggaa attttgaccg atatttacaa agcagactca gaatggaatg ttgtcttgct
     3541 tcgttacttt aacccaatcg gagcccatga gagtggtgat ttgggagaaa atccaaacgg
     3601 tattccaaac aatctcttgc catatgtgac tcaagtagcc gttggaaaat tagagcaagt
     3661 gcaagtgttt ggagacgatt acgatacgga agatggaaca ggtgttcgtg actatatcca
     3721 cgttgtcgat ttggctaagg gtcacgttgc agctttgaaa aaaatccaaa aaggttcagg
     3781 actaaacgtt tataaccttg gaactggtaa aggttactca gttcttaaaa ttatccaaaa
     3841 catggaaaaa gcggtgggac gtcctattcc ttaccgcatc gtagaacgtc gcccaggtga
     3901 tatcgctgcc tgctactcag acccagcaaa agctaaagca gaactcggtt gggaagcaga
     3961 actcgacatc acccaaatgt gtgaagacgc atggcgttgg caaagcaagc atccaaatgg
     4021 atttgaagac taagatgatg atttcaatca tcgtcccttg tttaaacgaa gaggaagtac
     4081 ttcctctttt ttatcaggct ctggaagctt tacttccaga tttggaaaca gaaatcgagt
     4141 atgtctttgt cgatgatgga tcaagtgatg ggaccttgga actcttaaag gcctatcggg
     4201 agcaaaatcc ggcagtccat tatatttctt tctctcgaaa ttttggcaaa gaagcagccc
     4261 tttatgcagg cttgcaatat gcgacaggag atttggtggt ggtgatggat gcagacctcc
     4321 aagatcctcc tagtatgttg tttgagatga aaaatgtact agacaaaaat gtagacttgg
     4381 actgcgttgg gacacggaga actagtcggg agggagaacc cttctttcgc agtttctgtg
     4441 ctgttctctt ttatcgcctc atgcaaaaaa tcagcccagt agctctgccg tcgggtgtcc
     4501 gtgattttcg tatgatgaga aggtctgtgg tcgatgccat tttaagcttg actgagtcca
     4561 atcgtttttc taagggactc tttgcctggg tcggctttaa aacccactat ctggactatc
     4621 caaatgtcga aaggcaggct ggcaagacca gttggagttt taggcaactc tttttttact
     4681 ccattgaagg gattgttaat ttttcagatt tccctttgac tatagccttt gtagctggtc
     4741 tcctatcttg ttttctttct ctgctgatga ccttttttgt tgtggttcgg accctcattt
     4801 tgggcaatcc gacatctggt tggacctctc tgatggctgt tattctcttt cttggaggca
     4861 ttcaactctt gaccattggg attctcggta agtatatcag taagatttat ttagagacta
     4921 aaaaaagacc actttatctt atcaaagaaa aaagtgacct tcctgatttt acagaaaaaa
     4981 taaagtgaaa agactataat tttacatgga aatgtgctaa actagaggga gtggattatc
     5041 gccattgatt taggcttcct ggtttccaat cagggcgcga gttgggcaat ttttaacagc
     5101 ctctaagaca tcctgactag gagaaatttc tttttccagt tggtcagggt catcgtaaaa
     5161 acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa taagtttggc aaagtccaca
     5221 ggcgatacat cgttcaggta taagtgtgat tttcatgttc atatttatat tgtaataaga
     5281 aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac cgtggcaaga agatatttat
     5341 gatcaagaag aatcaagagc agggcgtcgg catcgaaacc acggaggggc tgataggatg
     5401 gctaatcgta ttttgacgat cctagctagt attttctttg taattgtggt ggtgatggtc
     5461 atcgttctca tctatctatc atcggggggg agcaatcgca cagcagcctt aaaagacttt
     5521 catgattctg atgcaagtgt agtacaaatc tcatcttcaa gcagttctca gcctgagcag
     5581 agttcagagc cagaatctac ttctagtagt tcagaagaag ctgctaatcc tgaaggaacg
     5641 attaaagttc tcgcaggaga aggggaagca gctattgccg ctcgtgcagg aatctccatt
     5701 gctcagttag aggccttgaa tcctgggcac atggctacag gatcttggtt tgctaatcca
     5761 ggtgatgtta taaaaataaa ataggagccg atatgaaaac aattcaaatt gctattgatg
     5821 gtcctgcttc cagcggtaag agtacggtcg caaagattat tgctaaggat tttggattca
     5881 cctaccttga tacaggagct atgtatcgtg cagcgaccta tatggctctt aagaaccaat
     5941 taggagttga agaagtcgaa gcccttctag ccttgttgga ccagcatcca atcagctttg
     6001 gacgttcaga aactggagac cagcttgttt ttgtaggaga tgtggatatt acccatccta
     6061 tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc agcaattcct gaagtgcgtg
     6121 agaaactggt ttctctccaa caagaaattg cccagcaagg cgggattgtc atggatggtc
     6181 gcgatattgg aactgttgta ttgccacaag cagaattgaa aattttccta gtagcttctg
     6241 ttgatgagag agcagagcgt cgttacaagg aaaatattgc caagggaatt gaaacagacc
     6301 ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa ggatagtcat cgtgagactt
     6361 ctcctctcaa acaagcagag gatgctgtct acattgatac aactggtttg aacattcaag
     6421 aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat gtaaatgtag aaaagccgat
     6481 agattgatat cggttttttt ctaaatttgt caaaatgctg attttagggt ataatagttg
     6541 ctgttcgaga aattttgatt ttcaaagaat agtgaatgat gataaggaga aaccatgaac
     6601 aatttaccaa attgtccaaa atgtaactca gagtatgtct acgaagacgg tgccctactg
     6661 gtttgcccag agtgtgctca tgagtggaat cctgctgaag ttgcagaagt agaagaaggt
     6721 cttgtcgcta tcgatgccaa cggaaataaa ttgactgatg gtgatacagt aactctcatc
     6781 aaggacttga aagtaaaagg tgcgccaaaa gatttgaaac aagggacgcg cgtgaaaaat
     6841 atccgcatcg tagaaggcga tcacaatatc gactgtaaga ttgatggttt tggtgccatg
     6901 aaacttaaat cagagtttgt gaggaagatt taagcatgtc aaaacactat aaacttgtat
     6961 tttatagccg tatcttcttg tttctagcgg cttttacggg agtttatctt gaaattacta
     7021 ggcatggtgg ttttgggatg cttctctatt acacggttct gtccaacctc ttggtaacta
     7081 tttttaccct ttatcttcta aaggttatga gccgtgtagg tgaaaattgg caaaggccaa
     7141 gtctcttgcg cttaaaaggt ggggtcacca tgagtatcat gattacctgt gtgatttacc
     7201 atttcctctt ggcgcccatt gcgactaatt tctataccct agaaaatttc ctttgccact
     7261 atatcgttcc catctggttt ttagcggata ccctcttttt tgacaaacag ggtcaataca
     7321 agatttggga tccagcagtg tggaccattt taccctttct gtatatgatg tttgctcttt
     7381 ttaatggctt ggttctaaaa ctcaatattc caaatgccaa ggataatcct ttcccttact
     7441 tctttttgaa tgtgaacaag ggttggaatg ttgtgtttaa gtggtgtctg attatctttg
     7501 ttgcctatat ggtagcagga tttattttct actttatcaa gcaaatcaag agaaagtcat
     7561 cctaatactc ttcgaaaatc tcttcaaacc acgtcagttt ccatctgcaa cctcaaaaca
     7621 ctgttttgaa caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
     7681 gatacaaggg attcattttc gaatttaaga aggagtctac aaaccaatga ggcttcttct
     7741 tttcttgctt atttgataac tatcaaaaaa gtagaagatt aagagaaata tagaaagaga
     7801 tttcatgaaa caatttttag aacgggccag cattttggct ctctccctcg ttttgattac
     7861 ctccttttcc atttcgagtg ccctaccagc catgtttgac tattatcagg gttattctaa
     7921 ggaacaaatt gagctcttgg tgagcttgcc ttcctttgga atcatgatga tgttactgct
     7981 aaatggtttc ttagaaaaaa tatttcctga gcgcttacag attagtttgg gcttgctgat
     8041 tttatcattg agcggtacag ctcccttctg gtaccaagcc tatccctttg tctttggaac
     8101 acggcttctc tttggtttgg gtcttgggat gatcaatgcc aaggccattt ctattatcag
     8161 tgaacgctac caaggaaaaa ggcgaattca gatgttaggg ctacgcgctt ctgcagaggt
     8221 cgttggagct tctctcatta ccttggccgt cggtcagttg ttggcctttg gttggacagc
     8281 tatctttcta gcctatagtg ctggattttt ggtgctgccc ctttatctgc tctttgtccc
     8341 ttatggaaaa tcaaagaaag aagtcaagaa aagagcgaag gaagcaagtc gtttaactcg
     8401 agaaatgaaa ggcttgattt ttaccttagc tatcgaagcg gcagttgtag tttgtaccaa
     8461 tacagctatt accatccgta ttccaagttt gatggtggaa agaggattgg gggatgccca
     8521 gttatctagt tttgttctta gtatcatgca gttgatcggg attgtggctg gggtgagttt
     8581 ttctttcttg atttctatct ttaaagagaa actgctcctc tggtctggta ttacctttgg
     8641 cttggggcaa atcgtgattg ccttgtcttc atccttgtgg gtggtagtag caggaagtgt
     8701 tctggctgga tttgcctata gtgtagtctt gacgacggtc tttcaacttg tctctgaacg
     8761 aattccagct aaactcctca atcaagcaac ttcatttgct gtattaggct gtagtttcgg
     8821 agcctttacg accccattcg ttctaggtgc aattggctta ctaactcaca atgggatgtt
     8881 ggtctttagt atcttaggag gttggttgat tgtaatctct atctttgtca tgtacctact
     8941 tcagaagaga gcttaggatt gattcctaag tttttctttt gatactcaat gaaaatcaaa
     9001 gagcaaacta tagttgattg agtttggaat agtacgctgt agctgctaaa atatttctag
     9061 aaattaattt gactttccta atagagttgt tcatatctta tttcaatcta ctatagaaag
     9121 ctagccgcag gttgctcaaa atagtgtttt gaggttgcag atggaaactg acgtggtttg
     9181 aagagatttt cgaagagtat gagattttta tacccataca atatttgttt taggtccttg
     9241 tttacagact aatttcttga taaaatagag gttatgacga gatataaagc aactatttcc
     9301 tatgatggtt atgcctttgc tggctttcag cgccagcctc atgcgcgtag cgttcaggaa
     9361 gaaattgaaa aaaccttgac cagattaaat aaagggcaaa ccattactgt tcacggtgct
     9421 ggtaggacag atagtggggt tcatgccctg ggacaggtca ttcattttga cctgccttat
     9481 cagatggatg aggagaaact ccgttttgcc ttggataccc agtctcctga agatattgat
     9541 gtgatttcga ttgagcttgt ggcagctgat tttcattgcc gttatgccaa gcatagcaag
     9601 acctatgagt ttattgtgga tagaggacgt cccaaaaatc cgatgcgccg tcactatgcc
     9661 acccactttc cctacccact cgatgtggaa cgaatgcaga ttgcaatcaa aaagctagag
     9721 ggaacccatg attttaccgg ttttacagcc tctgggacta gtgtagagga taaggttcgc
     9781 accatcacag aagctagttt aatagtcgat gagacaggac aatttttgac ctttaccttt
     9841 tccggaaatg gtttcttgta taaacagatt cgcaatatgg tggggactct tctcaaaatc
     9901 ggtaacaacc gcatgccagt agagcagatt gatctcatct tggagaagaa ggacaggcaa
     9961 cttgcaggtc ccactgcagc accaaatggt ttgtatttaa aggagattcg ttatgaagaa
    10021 taatcgtatt ttagcacttt ctggaaatga tatttttagt ggtggtggac tgtcagctga
    10081 tttggctacc tataccttga acggcttgca tgggtttgta gcagtgactt gtttgacagc
    10141 cttgacagaa aaaggatttg aagtctttcc aactgatgat accatttttc aacatgaatt
    10201 agatagcttg cgtgatgtgg aatttggggg aattaagatt ggtcttctcc ctactgtcag
    10261 tgtggctgag aaggccttgg actttatcaa acaacgccca ggagtacctg tggtgttgga
    10321 tcctgtcttg gtctgcaagg aaacgcatga tgtagctgtc agtgagctct gccaagagtt
    10381 gattcgcttt ttcccttatg tcagtgtgat tacgcctaat ctcccagaag cagaattatt
    10441 atccggtcag gaaattaaaa ccttggaaga catgaaaact gcagcgcaga aattgcatga
    10501 tttaggagcg ccagcagtca ttatcaaggg aggcaatcgt cttagtcagg acaaggctgt
    10561 ggatgtcttt tatgatggac agacctttac tatcctagaa aatccagtta tccaaggcca
    10621 aaatgctggt gcaggttgta cctttgcctc tagcattgcc agtcacttga ttaaaggtga
    10681 taaacttttg ccagcagtag aaagctctaa ggctttcgtt tatcgtgcta ttgcacaagc
    10741 agatcagtat ggagtaagac aatatgaagc aaacaaaaac aactaaaatc gcccttgtat
    10801 ccctattaac cgccctttct gtggttctag gttatttctt aaaaatccca acacctacag
    10861 gaattctaac tcttttagat gctggtgtct tctttgcggc cttttacttt ggtagtcgtg
    10921 aaggagcggt agtcggagga ctagcaagtt tcttgattga cctcttatca ggctaccctc
    10981 agtggatgtt ctttagcttg gtcaaccatg gcttgcaggg atttttcgca ggatttaaag
    11041 gaaaaagtca gtggttaggc cttattttag caactattgc catggtagga ggctacgcct
    11101 tgggttctac tttgatgaat gactgggcag cagccctccc agaaattcta ccaaatttca
    11161 tgcaaaatat ggtagggatg attgtaggat ttattcttag tcaaagtatc aagaagatta
    11221 agtaatactc ttcgaaaatc tcttcaaacc acgtcagctt gtccttagtg tgtatatgtt
    11281 actgacttgg tcagttctat ccttaacctc aaaactatgt ttcgaggagc cttccgctaa
    11341 cttcccagtc tgagtttcat tgaatattaa cgatacttta tcacataaag aggctggatg
    11401 aaaagtcttc acaatcaaaa aaacgcatca tatcaggtgt acatgaacta caccccaaaa
    11461 gttagacaga aaaaatctaa cttttggggt gtttttatta tgaaattaac ttatgatgat
    11521 aaagttcaga tctatgaact tagaaaacaa ggatatagct tagagaagct ttcaaataa
//
LOCUS       DAVLTM010000058        11438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLTM010000058 DAVLTM010000000
VERSION     DAVLTM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.059"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            107..865
                     /locus_tag="VYN16_001331"
     CDS             107..865
                     /locus_tag="VYN16_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1403853.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYTHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDMTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            876..1669
                     /locus_tag="VYN16_001332"
                     /pseudo
     CDS             876..1669
                     /locus_tag="VYN16_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            1682..2359
                     /locus_tag="VYN16_001333"
     CDS             1682..2359
                     /locus_tag="VYN16_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1403854.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQV
                     NKKAYSL"
     gene            2369..3028
                     /locus_tag="VYN16_001334"
     CDS             2369..3028
                     /locus_tag="VYN16_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1403855.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISNLSHMLAKRWQQAA
                     E"
     gene            3172..4362
                     /locus_tag="VYN16_001335"
     CDS             3172..4362
                     /locus_tag="VYN16_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1403856.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4683..5564)
                     /locus_tag="VYN16_001336"
     CDS             complement(4683..5564)
                     /locus_tag="VYN16_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1403857.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5705..7033)
                     /gene="vncS"
                     /locus_tag="VYN16_001337"
     CDS             complement(5705..7033)
                     /gene="vncS"
                     /locus_tag="VYN16_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1403858.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7030..7686)
                     /gene="vncR"
                     /locus_tag="VYN16_001338"
     CDS             complement(7030..7686)
                     /gene="vncR"
                     /locus_tag="VYN16_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1403859.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCIFTVRNVGYKLERK
                     "
     gene            complement(7735..7818)
                     /gene="pep27"
                     /locus_tag="VYN16_001339"
     CDS             complement(7735..7818)
                     /gene="pep27"
                     /locus_tag="VYN16_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1403860.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(7998..9377)
                     /gene="vex3"
                     /locus_tag="VYN16_001340"
     CDS             complement(7998..9377)
                     /gene="vex3"
                     /locus_tag="VYN16_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1403861.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9429..10076)
                     /gene="vex2"
                     /locus_tag="VYN16_001341"
     CDS             complement(9429..10076)
                     /gene="vex2"
                     /locus_tag="VYN16_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1403862.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10089..11366)
                     /locus_tag="VYN16_001342"
     CDS             complement(10089..11366)
                     /locus_tag="VYN16_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1403863.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQVSLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3230 a         2550 c         2166 g         3492 t
ORIGIN      
        1 gtcctgttct tatttcaata tagtgaattt tttcagatat ggtttgacaa gaacattctt
       61 ttattgtaga atcgtgttat aaaatctaac ataaaggaga tctctcatgg ctttagtaga
      121 atttaaaaac gtcgaaaaat attacggaga ctaccacgca ctccgcaaca tcaatctccg
      181 ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct ggctctggga agtccactct
      241 tatccgtacg atcaatggtt tagaggctgt tgacaaagga agtctcctag tcaatgggca
      301 ccaagttgct ggtgccagcc agaaagattt ggtacctctt cgcaaggaag tcggcatggt
      361 ttttcaacat tttaaccttt atacacacaa aactgtgtta gaaaacgtga cacttgcgcc
      421 cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa accgcccaaa aatatctgga
      481 atttgtaaat atgtgggaca agaaagattc ctatcccgcc atgctatctg gtggacaaaa
      541 acagcggatc gccatcgctc gtggtcttgc tatgcatccg gaactcctcc tctttgatga
      601 accaacatct gctcttgatc ctgagactat cggagatgtt ctagcagtta tgcagaaact
      661 ggcgcatgat gggatgaaca tgatcatcgt tacccacgaa atgggctttg ctcgagaggt
      721 tgcggaccgc attatcttta tggccgacgg agaagtttta gtagatatga cagatgtcga
      781 taactttttt gacaatccaa gcgaacctcg tgcccaacaa ttcctcagca aaattatcaa
      841 ccacgaaagt gacaaagtca aataaggagg cgcctatgaa aaaaaaattc tttttatcag
      901 cattattgat tagccttttc ggccttgctg ctgccaaacc agtccaggct gatactagta
      961 tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt caaacaagac gttcccaatt
     1021 ttggttacaa agatcccaag accggtactt attctggtat cgaaaccgac ttggccaaga
     1081 tggtagctga tgaactcaag gtcaagattc gctatgtgcc ggttacagca caaacccgcg
     1141 cccccttcta gacaatgaac aggtcgatat ggatatcgcg acctttacca tcacggacga
     1201 acgcaaaaaa ctctacaact ttaccagtcc ctactacaca gacgcttctg gatttttggt
     1261 caataaatct gccaaaatca aaaagattga ggacctaaac ggcaaaacca tcggagtcgc
     1321 ccaaggttct atcacccaac gcctaattac tgaactgggt aaaaagaaag gtctgaagtt
     1381 taaattcgtc gaacttggtt cctacccaga attgattact tccctgcacg ctcatcgtat
     1441 cgatgccttt tccgttgacc gctctattct atctggctac actaataaac ggacagcact
     1501 actagatgat agtttcaagc catctgacta cggtattgtt accaagaaat caaatacaga
     1561 gctcaacgac tatcttgata acttggttac taaatggagc aaggatggta gtttgcagaa
     1621 actttatgac cgttacaagc tcaaaccatc tagtcatacc gcagattaag gaggataccc
     1681 tatgacagat ttatcatctt ggacagccta ttttcaggat tttggacaat ttttcaatgg
     1741 tttcctcttc acccttgccc tagcgattgg atcctttatc ctcgccatgg tcttaggaat
     1801 cttctttggt gccatgtcaa ccagtaaacg tccaatttta cgcattttag ctcgcatctt
     1861 cgtggaattt taccaaaaca ctcccctctt ggtgcagttt gttattgttt tttatggcct
     1921 acctcttatc agtgaccata tcatcatgat tccaatttat tggacagccg ttctctgcgt
     1981 gggactctat cacggcgctt atatcgctga ggttattcgt tcagggattc agtctattcc
     2041 tagtggtcag atggaggccg ccttgtcgca aggttttacc tatatcagtg ccatgcgctt
     2101 gattatcttg cctcaggcct tccgcatcat tctccctcca ttgaccaacc aaattgttaa
     2161 cctcatcaag aacacatcta ctgtagctat catctctgga gtagacttga tgtttgtgac
     2221 taagtcttgg tcggctctca acggaaacta tatcccagcc tttttaggtg ctgctcttct
     2281 ctactttgcc ctatgcttcc ctgttgccca gtttggtcgc aagatggagc aagtcaataa
     2341 aaaagcctat tcactttagg aggttactat ggaatctatt ttagaaattt taaccccaga
     2401 taacctagtc tttatcttta aaggatttgg cttgaccctc tatatttctc tgattgccat
     2461 catcctctct actatcatcg gtaccgtgct tgccgtcatg cgaaatggaa aaaatcctat
     2521 cttacgcatt atttccagta tttatatcga gtttgtgcgc aacgttccca accttctctg
     2581 gatttttact atctttttgg tgttcaaaat gaagtccaca ccagcaggta ttacagcctt
     2641 tactctcttt acatcagcag ccttggctga gattattcga ggcggtctca atgccgtaga
     2701 caagggacaa tacgaagcag gaatgtcaca aggcttcacc tcagcccaaa tcctctacta
     2761 catcattctc ccacaagcca tccgcaaaat gctaccagcc atcatttctc agtttgttac
     2821 cgtgattaag gataccagtc tcctctactc tgttatcgcc ctacaagaac tctttggagc
     2881 cagccaaatt ctcatgggcc gttatttcga accagagcag gtcttcagtc tttacatcct
     2941 gattgccctc atctacttca gctttaacct agcaatttct aacctgtctc atatgctagc
     3001 aaaacgttgg caacaagctg cagaataaca aattagctct ctagttcatt ggagagtttt
     3061 ttgatgatag tagatattta tagacctcaa tttgacacca tacattttct atgataaaat
     3121 gtaacaaatt tatttacaac aaatcgaaat ctaaatgaaa tggaattttc tatgaaatca
     3181 atcaaaggac tactctttat catagctagt tttatcttga ctcttttgac ttggatgaac
     3241 acttctcccc aattcatgat tccaggacta gctttaacaa gcctatctct gacttttatc
     3301 ctagccactc gtctcccact actagaaagc tggtttcacg gtttggagaa ggtctacacc
     3361 gtccacaaat tcacagcctt tctctcaatc atcctactaa tctttcataa ctttagtatg
     3421 ggcggtttgt ggggctctcg cttagctgct cagtttggca atcttgccat ctatatcttt
     3481 gccagcatca tccttgtcgc ctatttaggc aaatacatcc aatacgaagc ttggcgatgg
     3541 attcaccgcc tggtttacct agcctatatt ttaggactct ttcacatcta catgataatg
     3601 ggcaatcgtc tccttacatt taatcttcta agttttcttg ttggtagcta tgccctttta
     3661 ggcttactag ctggttttta tatcattttt ctatatcaaa agatttcctt cccctatcta
     3721 gggaaaatta cccatctcaa acgcttaaat cacgatacta gagaaattca aatccatctt
     3781 agcagacctt tcaactatca atcaggacaa tttgcctttc taaagatttt ccaagaaggc
     3841 tttgaaagtg ctccgcatcc cttttctatc tcaggaggtc atggtcaaac tctttacttt
     3901 actgttaaaa attcaggcga ccataccaag aatatctatg ataatcttca agccggcagc
     3961 aaagtaaccc tagacagagc ttacggacac atgatcatag aagaaggacg agaaaatcag
     4021 gtttggattg ctggaggtat tgggattacc cccttcatct cttacatccg tgaacatcct
     4081 attttagata aacaggttca cttctactat agcttccgtg gagatgaaaa tgcagtctac
     4141 ctagatttac tccgtaacta tgctcagaaa aatcctaatt ttgaactcca tctaatcgac
     4201 agtacgaaag acggctatct taattttgaa caaaaagaag tgcccgaaca tgcaaccgtc
     4261 tatatgtgtg gtcctatttc tatgatgaag gcacttgcca aacagattaa gaaacaaaat
     4321 ccaaaaacag agcttattta cgaaggattc aagttcaaat aagtaatcat actctcctgg
     4381 gatttcccag gagagttttt tatagccaaa tgaaataaga ccggaacgat ggaattttgg
     4441 cattcaaatc aattgataag aatattttat agtggagtga aataggatac gaacaaattg
     4501 attaggaaaa tcaaatgaat ttatagaaat cttttagcag ttatgttgtc ctattctagt
     4561 ttcaaaacgc tatggaagca gcattgtgct agtctagatt cagtttacta tactaaaacg
     4621 agtagcttga aatcaaaaaa cccaccctca caggcaggtt ttatctgtat tattcagcta
     4681 gattatgctt taccttctga accgaatacg tcgatacgtt cttcaaccga tgcttggata
     4741 gcttttacac cgtcagccaa gaatttacgt gggtcgaaga gttttttctt gtcgtattct
     4801 gcttcgtttg cttcgtaatc acgagcaaat ttacgagttg cgttagcgaa tgcgatttgg
     4861 cattctgtgt taacgttaac tttggcaaca ccaagtttga tagctgcttg gatttgctca
     4921 tcaggaatac ctgatccacc gtgcaatacg attgggaatc ctggaagagc ttctgtcaat
     4981 ttttgcaagt ggtcaaggtc aagaccttcc cagtttactg ggtaagggcc gtggatgtta
     5041 ccgataccag ctgccaagaa gtcgatacca gtttcaacca ttgctttagc gtcttcgatt
     5101 ggagccaatt cacctttacc gatgattccg tcttcttcac caccgatagt accaacttca
     5161 gcttctactg agataccttt agcgtgtgct ttttcaacaa cttctttagc caatttaagg
     5221 ttttcttcaa ctggaaggtg tgaaccgtca aacatgattg aagtataacc aacttcgata
     5281 cactcaagtg catcttcgta gtgaccgtgg tcaaggtgga tagctactgg tacagtgata
     5341 cccattgatt caacaaggtt agcgatcaag ttgcgagcaa ctttgtaacc acccatgtat
     5401 ttagcagcac ccattgaagt ttggatcaaa actggagctt ttttagcttc tgctgcgcgc
     5461 aagatagctt gagtccactc aaggttgttt gtgttaaatc caccaactgc ataaccgttg
     5521 tcacgggctg cttggacaaa tttttctgct gaaacgattg ccattttatc aggcctcctg
     5581 tatattttta tgggtcatcc catttacatt gttcatttta tcactttttg ccaaaaaaat
     5641 ctagtttttc ccgcagtttc gattgatttt cttctaactc catctatgta aaccctttct
     5701 ctccctagtc ttggacgact tttggaaaat ctataaagaa ggttaaacga ttctcctcca
     5761 tctcgaaacg ataagctaat ttttcatgtt ctaatagact cttaaccaca aaaagcccca
     5821 taccagaccc cttgaccttg cgactggcat tgtcagaaaa agactgggct agtttttctt
     5881 gttcctctga gctacagcta ttttcgataa aaagttctcc ttctctttct ccaattcgaa
     5941 ctaagccacc tggaacagag tgcttaatgg cattgctgat gagattagaa agaatcaact
     6001 tcataactga tgggtttaga taagcctgct gatgggtcaa actattgtct atctggagct
     6061 ctctttcctt ggctagcaag gcataatctt tgaccagatt tttcgtcatc tggaggaggt
     6121 caattgtttc cctatcatct cgcaattcct gcacagaaga gagggaaagt atctgcagaa
     6181 catggtgatt gagttcatcc acaatcccca aggcaactcc cagatactgg tctctatcct
     6241 tataacgacc gatattctct ctcatatttt cgattaggat tttcaaacta gccagcggtg
     6301 ttttcaattc atgagaagct cctcgtagga attcgacctt catcttctcc agctggagaa
     6361 tggcttcatt cttttcatgc aagtccgcaa taacagtcaa gagatgctgg tagaggctat
     6421 tgatttgttc cttgagatta cctatctcat ccttagaatc cacgcgcaat cgcacttggg
     6481 aatccaggtc catcatccga cgggtcaccc gcttgatttc caaaatcggt gcaacaatag
     6541 tccgagcgta gatgtaggcc accaagaggg aaatcagaaa ggaggccagc aaggtatagg
     6601 gaagaaactg gagactgatt tgctccgctt ccttttgtaa atccatggaa gctagaaact
     6661 ggagaatcat agtaccaccg tcttgcgttt tcacctcgcg ctcctcaata aagagagagg
     6721 ttgtctggcg gtctgtgtcc agaggaagac tgtccttgac ttctaacttg tcctcggtca
     6781 tctcaccttt gacggtcccc ttgatatcac tagtctggga atacaagtct aacacttgct
     6841 cgatactctg cctatctttc ccttctaggg actgggcaat ggctgttgcc ttttgaccaa
     6901 tggtttcctg acgatgactc agataagtcg aaggaaaaag aaaataaata gctaaatgaa
     6961 ggcagataac cagaacacta aatatcgaga aggtatagat aaatatcttt gtaaataaac
     7021 ctgttcgttt cattttcgct ccaatttata accaacattg cgcacagtga agatacaatc
     7081 caagtctagc tttttccgca attccttgat ataaacatca ataacacggt caaagggaac
     7141 ctcatctgtc gctttccaga cggcatcgat aatctgagac cgagtcaagg cccggccttc
     7201 atttttcact agatagtcca gaatttccaa ctctttggca ttgataggca cttcttgacc
     7261 tgcgaggctt gcactgtagc tttcaaagtc caccttggta tccttgtaag aaaagattcg
     7321 tcctgtatcg tagtagcgct tgaaaatcgc gtccaccctc acttttaaga gggagaggga
     7381 gaaaggtttt tccagatagc catctgccaa agaggcaaag gcactcatct tgtattcctc
     7441 atcctgaaaa gctgtcaaca tcaagacagg aacctgactg gttttacgaa tctcagctag
     7501 gacttctaag ccgttaagct tgggcatctg gatatccagt aaaaccaggg ccacctcata
     7561 gctagaaaat tgctccagag cttcctgacc gtccgctgcc tcaatagttt catagccaca
     7621 atccgtcaaa taatcactga ccccctcacg gatcatctct tcatcttcta caattaaaat
     7681 tttcatactt taactgctct ctatttttta tttttcttag aataaatacc tactctattt
     7741 tctattatag tctcttgctg gccttttgtc tgtaagcaac tgaccactag ataaaacgtt
     7801 gtgaaattcc tttctcataa attccataac tttagtatat tatatttaag cactaaagta
     7861 caaagaaagc aactgaaagc aatgattttc accactgctt tcagatttat tttgaattgt
     7921 taaatagcta ttcctatcca ctattcttga atagaaacac aagatgcaat ctttattcca
     7981 gactcatttt ttcaaattta ttcaccatcc agcaagagct cttttggttg ttttctaagg
     8041 agattgcttg aagcaagcgc cataacgaga accactagaa ccaaggcaag gacaaaaatg
     8101 atgataaagt ctgatgtctg aatggaaatg tctaggctcg acaaggtctt gctaaagcca
     8161 tctacttctg caccgccacc aaggttagag gcttgagccg ccttactagc ctgtttggca
     8221 acacctgaag tcacattggc aaggacagtg tttccaattg cacgggcagt gtaattagct
     8281 aggaagtaag cagaaactag agcagggata gcaatcaaga tagattcggt gatgaattga
     8341 cccaagatac ttgcctgctt gaggccgata gagaggagaa ttcccacttc cttgcgacgg
     8401 gcgttgatcc aaaggctgag caagagggca aggaggagaa ctgagaagct caagctaccc
     8461 cagaagagga ggttggccat cttgtacata ccagagatag attgctcaag agctgggtag
     8521 ttagaggagc tcttgacgag tgtgtagctc ttccagttga taccactgat gccattcaac
     8581 tctttcataa catcatccaa gttcttgtct gctgttacaa agaaggttgc gtccccataa
     8641 atggctgtgt cttctgtgta tccataaagt tttgcagcag tgtgaatgtc tgtaatagct
     8701 gtgttttcgt aaagttcttg tgagtaggtt actgctgact tattatgacc atcaaagagt
     8761 cccttgattg tcacttcaac tgtttccttg gctccttttt cattatctgc atcgtagata
     8821 ttagagtcca gtttaacctt gtcccctact ttccagccgt gtttggctgc caagtccttg
     8881 tgcaagagga ttttatcctt gtcgtcgttg gttaagtgct ctccttcgac tagtttataa
     8941 gaaccagaga caaacttgtc ttctttagag gagtcattga cacctgtaat catcaagcta
     9001 cttccaaaac gtttggcacg atcagcagtg agattcttct tggtttctgg cgtttcaatc
     9061 aggtcatatc cagtcaaatc tccgatagcg ttgatacgtt tgacataaga ctcaatggcc
     9121 ttgttttcgg tgattttttt gatatcttca cccttgatat tcccagcacc acgaggcgtt
     9181 ccttggttga cgcgacgatt gatttgcatg gagaagctat tggtgatatt tttaaaggtc
     9241 tcctgagaag ccttggcagt agctcccttg attgacaagc cgaccaaact caagctcgcc
     9301 atgaggagaa taatcaggaa gatgacaatc gatttgaaaa acttccttgt aacataggca
     9361 aatgcgttgt gtaacataga ttccctttct agattttgtt ttaatcattc tatcaaaata
     9421 agctcaaatt atttactagt attgcgcgtt tcagtcagtt tcttatcctt taattcaagt
     9481 gtaatatctg acgcttgtgc cacttcttta ctgtgagtta cgacaatcac acatttacct
     9541 gttttctggg caagtgattt gagtagttcg acaatatctc cagcagtttt aggatccaga
     9601 tttcctgttg gctcatcagc tagaataact ggagcttctg agaccaaact gcgagcaatg
     9661 gcaacacgtt gctgttgacc acctgataac tggagaacat tccgcttgat ctggctttca
     9721 tccaaaccaa gctcaagaag tgtattcttg cttgcctttt tgttgaccaa tcggatattt
     9781 tccagcggag aaagataatc tatcaagtta taattttgaa agaccaggga aatatggtgc
     9841 atgcgatggt aagaatagcc cttcttacga atatcctctc cttgaaaaag gatagaacct
     9901 tcaacaggac tatctagacc agcaagtagg gacaagagtg tggattttcc tgctcctgac
     9961 tccccaataa tactgtaaaa ttttccgggt tcaaaattat aatcgatctg atataggact
    10021 gcttcagcag tattcttata acggtaggta acatcttgta attgtaataa agtcatgatt
    10081 tctccttctt aactaataga tgataaaatt tctttcggtg attttctaaa taagaatagg
    10141 aaacaaaggg ctacagataa gcaactaagc agaactagaa aaacatagga ttctgcaaaa
    10201 gataagatgc tagttgataa actgcttgct ttggctagtg tatcttgtaa gcttacctga
    10261 tctccacttg ctagtagagt ttggagtagg taagttgtga ttccgtttcc tgcaacaaat
    10321 gctggaagca aagctccaag agataccaaa actacctcta aacagaattg taggaagatc
    10381 gagctcttgc cttttccaag tgcaagtaaa atccccactt catagacccg ttctctcaac
    10441 cagagagaca aaaccagaat taaggctcca gctcctgcta tcaacatccc ataaaggaag
    10501 atggtcagga aggtttggaa agttgcaact gagtctttga tttgttcaaa agccttgttt
    10561 tccttttcga cttggtagcc ttgattttcc aaggccaagt tttctacctg cttcatgagt
    10621 ccgtccattt ccttaggatt ttctacatag aagcgtgctg cactgacttg agcttcacta
    10681 ttgcccaaaa gggtttggct actttcataa tctgtaaaga cttgattttc actgaagtca
    10741 gaagacaagc ctgtgaattt ctcttgtttt ttaccagaaa agatgccgat aatctcaaac
    10801 tctactgttt gtccttttcc agattcagac tgaccagcat ccaagccaat cttgtcatga
    10861 agtgaaagac cgttcttctt agccaattct tcgtggataa ggattttctt ggaatcccct
    10921 ttttgaaggt gtcgcccttc ttttagattg aaagccgaac tggtaaaggt tacatccttg
    10981 gatgaatcct caagagccgt taagctaacc aagttattgt ctgcagctga taaatcatca
    11041 cgctccacgc tctgctcgcc agtcactgct tccttgtctt ttagttttgc gaccgtctca
    11101 agttcaggag agacattttc cagcccctta atcttgctta cagatgctag gtctgacaac
    11161 ttgaatgtct gaccattctc tatcttctta atagaaaaag atgtattgag tgatttataa
    11221 agattgcttt ctactgtttt gttggacttc atcagagtca aacaggctga aattccggcc
    11281 aataagacca ataaaatcag aaataaaata aaacttctca gtcgctttct gctgacataa
    11341 gcccaagatc tttggattgg attcatttgt cacctccata tttgtaagac tattataaaa
    11401 cccaaatatg aaatgtttat gaaatacgaa aaaaaaaa
//
LOCUS       DAVLTM010000059        11342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLTM010000059 DAVLTM010000000
VERSION     DAVLTM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.060"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            3..445
                     /locus_tag="VYN16_001343"
                     /pseudo
     CDS             3..445
                     /locus_tag="VYN16_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826702.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            483..785
                     /locus_tag="VYN16_001344"
     CDS             483..785
                     /locus_tag="VYN16_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1403864.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            793..1595
                     /locus_tag="VYN16_001345"
     CDS             join(793..1243,1243..1595)
                     /locus_tag="VYN16_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1403865.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHESNLIRRSRWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSQGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            1644..2108
                     /locus_tag="VYN16_001346"
     CDS             1644..2108
                     /locus_tag="VYN16_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1403866.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2266..2691
                     /gene="lacA"
                     /locus_tag="VYN16_001347"
     CDS             2266..2691
                     /gene="lacA"
                     /locus_tag="VYN16_001347"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1403867.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2722..3237
                     /gene="lacB"
                     /locus_tag="VYN16_001348"
     CDS             2722..3237
                     /gene="lacB"
                     /locus_tag="VYN16_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1403868.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3248..4177
                     /locus_tag="VYN16_001349"
     CDS             3248..4177
                     /locus_tag="VYN16_001349"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1403869.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4179..5159
                     /gene="lacD"
                     /locus_tag="VYN16_001350"
     CDS             4179..5159
                     /gene="lacD"
                     /locus_tag="VYN16_001350"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1403870.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5393..6229
                     /locus_tag="VYN16_001351"
     CDS             5393..6229
                     /locus_tag="VYN16_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1403871.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6265..6582
                     /locus_tag="VYN16_001352"
     CDS             6265..6582
                     /locus_tag="VYN16_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1403872.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6584..8278
                     /locus_tag="VYN16_001353"
     CDS             6584..8278
                     /locus_tag="VYN16_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1403873.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTVKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8493..9899
                     /gene="lacG"
                     /locus_tag="VYN16_001354"
     CDS             8493..9899
                     /gene="lacG"
                     /locus_tag="VYN16_001354"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1403874.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9942..10118
                     /locus_tag="VYN16_001355"
     CDS             9942..10118
                     /locus_tag="VYN16_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1403875.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQ"
     gene            complement(10402..11163)
                     /locus_tag="VYN16_001356"
     CDS             complement(10402..11163)
                     /locus_tag="VYN16_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1403876.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3641 a         1956 c         2283 g         3462 t
ORIGIN      
        1 ttgtgttttg cttagaagcg gataatcaag aataactttt tgatcaggta gcaagcttgt
       61 tggaagaacg agaaattgtc actccaactt atcgtgaagc cttgatcacg cgtgaaaagt
      121 catttccaac tggtttagat atgaaatttt taggaaagga cttgcaaatg taatgattcc
      181 tcacacagat attgttcata atctagctga gaaagtggtg gttgttcgat tagagaaatc
      241 ggtgactttc cataatatga ttgcgccagg taaggaagta gaagtatcct tgctcttctt
      301 tatcatcaat aattcaagtt caagtcaaac gaacattctt gctcagttga tggacttttt
      361 cacaggaaat ggacatcttg aagacctatc aaaaatttcc gaaccagaaa aactttatgc
      421 ttatatagat gaagcaatcg cttaatcttg tctattaaaa aataaaatcg gaggaagtct
      481 atatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
      541 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg
      601 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
      661 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattctgta
      721 tcccagcaat gagcgttcca gtatttgaca atattagact tcctgcgaaa caaaatatgg
      781 tatagtagtt ccatgaatta tgaagcaagt aaacaactaa ctgatgcacg atttaagcgt
      841 cttgttggtg ttcaacgcac gacttttgaa gagatattag ctgtattaaa aacagcttat
      901 caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ctttcttatg
      961 gccactcttc aatatgtgcg agaatatcga acttatgaag aaattgcggc tgattttggt
     1021 atccacgaaa gcaacttaat ccgtcggagc cgatgggttg aagtaactct tgttcaaagt
     1081 ggtgttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg
     1141 gaagtacaaa tcaatcgccc taaaaaagaa ttagcgaatt attctggtaa aaagaaatgc
     1201 cacgctatga aggctcaagc gattgtcaca agtcaaggga gattgtttct ttggatatca
     1261 ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag tcgcagaaat atcggacaag
     1321 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     1381 aaactccacg taaatccagc aaactcaagc cgctaacagc tgaagataaa gcctgtaacc
     1441 atgcgctatc caaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta
     1501 aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga
     1561 gtgctggtat tatcaatcat gaactaggat tctagttttg caggaagtct aataaagaaa
     1621 ctagcaaggt cagaccttac tttattggtg gacatccagg atttaattgt cttctacttg
     1681 atgatgaagt ctatgaagat tattatctag agtttgagaa agaagagatt tgctctgttc
     1741 cacgtccttt cccagaaaca ggtatgttgg atttccaaga tagaagtcca tggctagagg
     1801 gacaaaaaga aatagatctt agttatgatc ttttcagcac agatgcagtg actttggatg
     1861 aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca tgataaggga ttgaaagtgc
     1921 attttgcaga atttccaaac ctcatcatct ggtcaacttt gaacaaggga cctttcatta
     1981 cctttgaacc atggtctggc ttgtcaacat tccttgaaga aggagatcat ttagaagata
     2041 agaagaatgt ttgtctctta gaagccaatc aggttgaaga attagggttt gagatagaag
     2101 ttttataaaa aaagtaaaaa ataaataaca aaaacaaaca caaactgttg acttcttcaa
     2161 acaatagtag tatattatgt ttgtgaggac taattgactg ttttttaaag acagccaata
     2221 cactgctgga atccagcata gaaaaaataa aaaggagtat acaatatgtc tattgttatc
     2281 ggcgcagatg ctgcaggttt gagattgaaa gaagttgtga aagacttctt agaaaaagaa
     2341 aacttccacc ttgtggatgt tacagctgaa ggtcaagact ttgttgatgt gactctcgct
     2401 gttgctgcag aagtaaacaa agaagaacaa aaccttggta tcgtgattga tgcttatgga
     2461 gctggtccat ttatggttgc aactaagatc aaaggaatgg ttgctgcaga agtatctgac
     2521 gaacgttcag cttatatgac tcgtggccac aacaactcac gtatgatcac tatgggtgca
     2581 caacttgttg gtgatgaatt ggctaaaaac atcgctaaag gatttgttaa cggtaaatac
     2641 gatggtggac gtcatcaaat ccgggtcgac atgttgaaca aaatgggcta attagattac
     2701 agtaagaaag gtaagttaaa aatgagaatt gcaattggat gcgaccacat cgtaacagat
     2761 gaaaaaatgg cggtttcaga atttttgaaa tcaaaaggat atgaagtcat tgactttggt
     2821 acatatgacc atacacggac tcactaccca atctttggta aaaaagtagg ggaagctgta
     2881 actagcggcc aagctgatct tggggtatgt atctgtggta ctggtgttgg tatcaacaac
     2941 gctgtaaata aagttccagg tgttcgttct gccttggttc gtgatatgac aacagccctt
     3001 tatgctaaag aacaattgaa cgctaacgtt atcggctttg gtggtaagat tactggtgaa
     3061 ttgcttatgt gtgatatcat cgaagctttc attcatgctg aatacaaacc atctgaagaa
     3121 aacaagaaat tgattgcgaa aattgaacac cttgaaagtc acaatgctca acaaacagac
     3181 gcaaacttct ttacagaatt ccttgaaaaa tgggatcgcg gagaatacca cgactaagag
     3241 gtgacctatg attttaacag tcacaatgaa cccatccatt gatatttcct atcccttgga
     3301 tgagttgaag attgatactg tcaatcgtgt ggtggatgta accaaaacgg ctggtggtaa
     3361 gggactcaat gttacccgag tactttcaga atttggcgat tctgttcttg ctactggttt
     3421 agtgggtggc aaacttggtg agtttttggt tgaacatatc gataatcaag taaagaaaga
     3481 tttcttctca attcagggag aaactcgtaa ctgtatcgct attctccacg gagacaacca
     3541 aacagaagtt cttgaaaaag gtcctgaagt attggaacag gaaggtcaag actttttgga
     3601 acatttcaaa aaactcttgg agtcagttga agtagtggcc atctcaggta gtctgccagc
     3661 tggccttcca gttgattact atgcgagctt ggtagaactt gctaatcaag ctggcaagcc
     3721 tgtagtcttg gactgctcag gtgcagcact tcaggctgtt cttgaatcac cccataaacc
     3781 aacagtcatc aaaccaaata atgaagaatt gtctcagctt cttggaagag aagtttctga
     3841 ggatttggat gaattaaaag aagtacttca agaacctttg tttgcaggga ttgaatggat
     3901 tatcgtttca cttggtgcca acggtacttt tgccaaacat ggtgacactt tctacaaggt
     3961 agatattcct agaattcagg tggtaaatcc tgttggatct ggagactcta ctgtggcagg
     4021 aatttcttca ggacttcttc acaaagaatc ggatgcagaa ttactcatca aggcaaatgt
     4081 ccttggtatg ctcaatgctc aagaaaaaat gactggtcat gtcaacatgg ccaactatca
     4141 agctctatat gatcaattaa tagtaaaaga ggtataaaat ggctttaaca gaacaaaaac
     4201 gtgtacgctt agaaaaactt tctgatgaaa atggtatcat ctcagctctt gcatttgacc
     4261 aacgtggtgc tttgaaacgc ctcatggctc aacaccaaac agaagaacca actgtggccc
     4321 aaatggaaga acttaaagtc ttggtagcag atgaattgac taaatacgct tcatctatgc
     4381 ttcttgaccc tgagtatgga cttccagcaa ctaaagctct tgatgaaaaa gctggtcttc
     4441 tccttgctta tgaaaaaaca ggttatgaca caacaagcac aaaacgcttg ccagactgct
     4501 tggatgtttg gtctgcaaaa cgtattaaag aagaaggtgc agatgcagtt aaattcttgc
     4561 tttactatga tgtagatagc tcagacgaac tcaatcaaga aaaacaagcc tacatcgaac
     4621 gcatcggttc tgagtgtgtg gctgaagata tcccattctt ccttgaaatc cttgcttacg
     4681 atgaaaaaat tgcggatgca ggttctgtag aatacgcgaa agtaaaacca cacaaagtta
     4741 tcggcgctat gaaagtcttt tcagacccac gctttaacat tgatgtcttg aaagttgaag
     4801 ttcctgttaa cattaaatat gttgaaggct tcgctgaagg tgaagtagtt tatacacgtg
     4861 aagaagcagc agccttcttc aaagcgcaag atgaagcaac gaacttgcca tacatctact
     4921 tgagtgctgg tgtatcagct aaactcttcc aagatactct tgtatttgct catgaatcag
     4981 gtgcaaactt taacggagtt ctttgtggcc gtgctacatg ggcagggtca gttgaagctt
     5041 acatcaaaga tggtgaagca gcagctcgcg aatggcttcg cacaactgga tttgaaaaca
     5101 ttgatgagct caataaagtt cttcaaacaa cagcgacttc atggaaagaa cgtgtgtaag
     5161 aaagtcctcc tagtttagga acatgaatct aaaaaaattt aaaaaaagtt gtatgtaaag
     5221 gtttacaaaa caaccgactt gtgctatact taaatcacaa gttaatacaa ggtgagtgtt
     5281 actaagtaat attaggcatg atcacaggtg aattagaaat cagctgattt tctagttcat
     5341 ttgtggtcat tttttgtact catatacctt taagatataa aaggaggttg acatgtatcg
     5401 aattctaaat ccaatgaatc acaatgtctc gcttgttaga aatgataagg gagaagaggt
     5461 gattataatt ggtaagggga ttgcattcgg aaaaaggaag ggggatttga ttgctgaaaa
     5521 tcaggttgag aaaatctttc ggatgaagac cgaagagtcc agagaaaact ttatggctct
     5581 tctcaaagat gttccgcttg attttatcac agtgacctat gaaatcattg ataagctatc
     5641 aaagaaatat cattatccga ttcaagagta tctctatgta accttgacag atcatattta
     5701 ctgttcttat caagctctaa ctcaaggaag gtacaaggat agtaatctgc cggatatttc
     5761 cgctaagtat cctgtcgctt ttcaaatcgc aaatgaagct tttgaaattt accgtcagaa
     5821 gctagcagat cattttcctg aggacgagat tattcggatt gcttatcatt tcatcaatgc
     5881 cgaaggtgaa aatgaagtgg aacttgtgga gtcgattgat aagaggaaag aaattctcag
     5941 gaatgttgaa gaagttttaa cggactatgc aattcaacga actaaaaaga ataaccattt
     6001 ctatgatcgc tttatgatcc atttgaatta tttcttggat tatttagaca gatctagaga
     6061 tgataaccaa tcacttctgg atatggaaga tcatattaaa caatcctatc caaaagcctt
     6121 cgagattggt tccaagatct atgatgtgat tacgcaacat acgggtcttg atttgtataa
     6181 aagtgaacga gtttatctag ttctacatat ccaacgttta ttgtcataaa aatttattta
     6241 aaactatata aggagaattc tatcatgaat agagaagaag taacattgtt aggttttgaa
     6301 atcgtagcct atgctggcga tgctcgttca aaactattgg aagccttgaa ggctgctgaa
     6361 gctggtgatt ttgaaaaagc ggacgctctg gtagaggaag ctggtagctg tattgcagag
     6421 gctcaccacg cgcaaacaag tctattgact aaggaagctt ctggtgagga cttagcgtat
     6481 agtgtgacca tgatgcatgg tcaagatcac ttgatgacaa ctatcttgtt aaaagaccta
     6541 atgcaccatt taattgaact ttacaagaga ggagttaaat aaaatggata agttaataat
     6601 ttttattgag aaaggaaagc ctttctttga gaaattatcg agaaatatat acttaagagc
     6661 gataaaagat ggttttattt ccagtatgcc agcagtatta ttctcaagta tttttatact
     6721 aatagctgct gtgcccaata tttttgggtt taaatggtct gatgaacagt tagcttttat
     6781 tttgaaacct tacaactact caatgggaat tttagctctc ttagtagctg ggacaactgc
     6841 aaaatcgtta acagattcag ttaatacacg tagtatggag aaaaccaacc aaatcaacta
     6901 catgtcaact tttttagcag ctgttgttgg attgttaata ctggcagctg atccaattga
     6961 aggtggattt gctaatggtc ttttaggtac tagaggatta cttactgcat ttttggcagc
     7021 ttttattaca gtaaatatct ataaagtatg tattaaaaat aatgtaacca ttagacttcc
     7081 tgaagaggtt ccaccaaata ttgcacaagt ttttaaagat gttattccgt ttgccttatc
     7141 tgttctttca atatatggat tggatttaat tgttagaaat atttttggta caaatgtagc
     7201 agaatctgtt ggtaaaatat tagcccctct attttcagct actgatggtt atatcggttt
     7261 agctattgta tttggtgctt atgctttctt ttggtttgtt ggaattcatg ggccatctgt
     7321 tgtagaacct ttaattgtag ctatcagtta tgcaaatata gaagctaatg ttcagcttgt
     7381 gcaagctggt atgcacgcag ataaaatttt aaatccagtt actcaaactt ttgttgtaac
     7441 aatgggtggt actggtgcaa cactagtagt tccatttatg tttatgtggt tatgtaaatc
     7501 aaaaagaaat agaattgtcg gacgtgcgtc tgtagttcct actttctttg gcgtaaatga
     7561 accaatccta tttggtgctc caattgtttt aaatcctatc ttctttattc cgtttgttac
     7621 tgctcctatt atcaatgttt ggattatgaa attttttgta gatgttctac aaatgaatag
     7681 tttcagtatt attttacctt ggacaacacc tgctccaatt ggtattgtta tgggaacggc
     7741 gctggctcca ttgtcattcg tcttggctat aactttaatc attatcgaca ctttaatcta
     7801 ttatccattt gttaaagttt atgaccatca aattttagaa gaagaacgga aaggaaactc
     7861 ttcatctgaa ttgaaagaaa aagttgctgc aaacttcaac actgtaaaag cggatgctat
     7921 tcttgaaaaa gcaggtgtcg atgcagcaca aaataccatc actgaagaaa caaatgtcct
     7981 cgttctctgt gctggtggag gtacaagtgg actccttgca aatgctttga ataaggcagc
     8041 agcaaaatac aatgtccctg tgaaagcagc agcaggtggc tatggtgctc accgtgaaat
     8101 gttgccagag tttgatcttg ttatccttgc ccctcaagtt gcttcaaact ttgaagatat
     8161 gaaggcagaa acagataagc tcggtattaa acttgctaag acagaaggcg ctcaatacat
     8221 caaattaact cgtgatggaa aaggtgccct agcattcgta caagagcaat tcgattaagg
     8281 ctaggaactg tgaaacagtc tcccatcgtt acggaaatcg ctatggcgaa tttcctaatc
     8341 gccttattaa ttcgtcggta aaaagatatc gtttttacct cctcatgtca caattcagtg
     8401 atttggcaca agaagtgaga tggagaagca tggctcactg actcctctcc tctcactttt
     8461 actttattta aatcaagaaa taggtgaaaa aaatgacaaa aacacttcca aaagacttta
     8521 ttttcggtgg cgcaacagct gcttatcaag cagaaggtgc tacacatact gatggaaaag
     8581 gaccagttgc ttgggataaa tatcttgagg ataactactg gtacactgcc gaaccagcta
     8641 gtgattttta caatcgatat ccagttgacc tcaagctagc agaagagtat ggtgtcaatg
     8701 gtattcgaat ttctattgct tggtcacgta ttttcccgac tggttacggc caagtaaatg
     8761 ctaaaggtgt tgagttttat cataatttat ttgcagagtg tcacaaacgt catgttgagc
     8821 cctttgtaac tcttcatcac tttgacactc cagaagctct ccactcaaat ggagacttct
     8881 taaaccgtga aaatatcgaa cattttgtag actacgctgc cttctgtttt gaagaatttc
     8941 cagaagtaaa ctattggaca acctttaatg aaattggacc aatcggtgat ggtcaatatt
     9001 tggttgggaa attccctcca ggtatccagt acgaccttgc caaagtcttt caatcacacc
     9061 acaatatgat ggtgtctcat gcacgcgcgg taaaattgta caaagagaaa ggctataaag
     9121 gggaaattgg tgttgttcac gccctgccta ctaaatatcc tctagatcct gaaaatccag
     9181 cagatgttcg tgcagctgag ttggaagata tcatccacaa taaattcatc ttagacgcaa
     9241 cttatctagg tcgctattca gctgaaacca tggaaggtgt caaccatatc ttatcagtca
     9301 atggtggtag tttggatctt cgtgaagaag attttacagc attagaagct gcaaaagact
     9361 tgaatgattt cctaggaatc aactactata tgagtgactg gatggaagcc tttgatggag
     9421 aaactgaaat tatccataat ggtaaaggtg aaaaaggaag ctctaaatac caaatcaaag
     9481 gtattggtcg tcgtgtagct cctgactatg taccacgcac ggattgggat tggattatct
     9541 accctcaagg tttgtatgac caaatcatgc gtgtgaagaa agattatcct aactacaaga
     9601 agatttacat cactgaaaat ggtctcggct ataaagatga gttcgttgat aacactgttt
     9661 acgatgatgg tcgtattgat tacgtgaaac aacacttgga gatcttgtct gatgcgattg
     9721 cagatggagc taatgtaaaa ggttacttca tttggtcatt aatggatgtc ttctcatggt
     9781 caaacggtta tgagaaacgt tatggtctct tctacgtaga ttttgaaact caagaacgtt
     9841 atcctaagaa atcagctcac tggtacaaga aagtagcgga aactcagatt atagactagt
     9901 agaattagtc attagatata gaattttagt gagtcaaaaa gatgttcaaa gattttatcc
     9961 aatctattta tgaaaaagtt tatattataa atttcgaaaa atgctctcaa ataccgtgtt
    10021 tgacgagtga agaattgaaa agtctatttt taaattttat caatcctgaa gaatgggata
    10081 ctatctcctt tgattcagat tttatgccgt ttcaataatc gtaaccaatt tctcaaaaaa
    10141 gttaaatctt atatttagta ctctgcaaaa ctcttatcta atcacgttgc ttatactcaa
    10201 tgaaaatcaa agagcaactt taaactagga agcgagtcgc agatttctca atgcatagct
    10261 ttgaggaatt gggcaaaaag tctttgatat agaaaaagca tagtatcagg tgtttcaaca
    10321 cctgatacta tgctttttat tgtgggaaga tttacttttt ttcttctgaa attgagttgt
    10381 tacccaggct ctttcagttt attaaggctt gatgacttta atgtgtttag atagcttaaa
    10441 aaggattgaa tcacttagtt tagaatctga aacaatagta tcaagatttg atacattata
    10501 aaaagtataa aaatcaaact tattgaactt actatgatct gcgagtaaat attttttatt
    10561 agaattattt aaagcgatgc gttgagcctc tccctcttcc tcgctaaaag tagctagagc
    10621 tccgttttga ataccattac agctaacgaa agctttagaa aattggagat tagagagatt
    10681 ttgtagggtc aatgtgccaa caaaagcacc tgtaatatcg cgataatttc cacctatcaa
    10741 aatcaaatct gttaattttc gttcgcttaa aatcagaaaa acaggtagac tgttggttac
    10801 gacgcggata ttgtcaatag gcaactcacg cgcaaaaaac tctaatgttg ttcctggtcc
    10861 aatgaaaata gtttctcttt cttctactag actgcctgca aaatgggcta tttcttgttt
    10921 ttctgccgtt tggagggctt gtttttcaat atttgatcgc tcattagtca aaagggagtt
    10981 ggttcgaagt ttttcagctc caccatgcac acgaatcagc aaatctttat cagctaattc
    11041 ctgtaaatag cgccttgcag tcatatctga aacggctatt tcgtccataa tctgtttaac
    11101 tgttatggtt cctttactat ttactatctc taaaattttg gttaattttt cttgtttgag
    11161 catattatca cctcgtttcc tactactatc ttaccataaa ctagctcatc attcaaatac
    11221 aaaaacaaca aaataaaaca aaaacaaaaa tatcgaagtt tattttcaaa actttcgata
    11281 tttttattaa gttattattt tgttgtttct agtttacttt ttgatggtta agagtggtgg
    11341 ag
//
LOCUS       DAVLTM010000060        11320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLTM010000060 DAVLTM010000000
VERSION     DAVLTM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.061"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(150..1130)
                     /locus_tag="VYN16_001357"
     CDS             complement(150..1130)
                     /locus_tag="VYN16_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1403877.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTLEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1456..1659)
                     /locus_tag="VYN16_001358"
     CDS             complement(1456..1659)
                     /locus_tag="VYN16_001358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403878.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHGFEVVDETDEVS
                     SKHRFEVADRTDEVSNIYTVRRR"
     gene            complement(1687..3702)
                     /gene="recG"
                     /locus_tag="VYN16_001359"
     CDS             complement(1687..3702)
                     /gene="recG"
                     /locus_tag="VYN16_001359"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1403879.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLNGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3721..4824)
                     /locus_tag="VYN16_001360"
     CDS             complement(3721..4824)
                     /locus_tag="VYN16_001360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1403880.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4814..5182)
                     /locus_tag="VYN16_001361"
     CDS             complement(4814..5182)
                     /locus_tag="VYN16_001361"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1403881.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5221..6252)
                     /locus_tag="VYN16_001362"
     CDS             complement(5221..6252)
                     /locus_tag="VYN16_001362"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1403882.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6254..7285)
                     /locus_tag="VYN16_001363"
     CDS             complement(6254..7285)
                     /locus_tag="VYN16_001363"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1403883.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7366..9879)
                     /gene="secA"
                     /locus_tag="VYN16_001364"
     CDS             complement(7366..9879)
                     /gene="secA"
                     /locus_tag="VYN16_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1403884.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10025..10312)
                     /locus_tag="VYN16_001365"
     CDS             complement(10025..10312)
                     /locus_tag="VYN16_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403885.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10323..10973)
                     /locus_tag="VYN16_001366"
     CDS             complement(10323..10973)
                     /locus_tag="VYN16_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1403886.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10977..>11320)
                     /locus_tag="VYN16_001367"
     CDS             complement(10977..>11320)
                     /locus_tag="VYN16_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403887.1"
                     /translation="NFFFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFL
                     LAQLMMLYLLLSYLIALISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLI
                     LSRLEEKFKKG"
BASE COUNT         3191 a         2664 c         2158 g         3307 t
ORIGIN      
        1 aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcatttta ctatatttcc
       61 atgataaaac gcatagtatc aagttctctt aatcccctga tactatgcgt gtttgtaatt
      121 tttaagattt tgtgcttaga gtcgactcct tattttagat atttaaaagg aatctcactt
      181 ccacagagcc agttgtagac ttggtcatta acaaatacat tcatggcttc gtgagcatac
      241 tcaggcatga tacgataggt tttatcgcag gtcagacgat tataaatcgc aaactgggta
      301 atgggatagc aaacatcgtc gtccaagccc gtaatcatct taacctcacc ttggatacga
      361 tgggcaagat ttttgacatc gatataggca agggtcgcca tgatttcctc ctctgtttca
      421 tggaaggggt cgtgaaactt gaaataacgg aaaagttcgt cgtaagcctc gctagtatta
      481 ccaatctcaa tcacccgtct gaagtctgac aagaagggat aaatggcaac tgttttctga
      541 attcgaggat tgagcgctgc tgcaactaga gctagagccc ctccttgtga ggcaccatag
      601 ctagaaagac gcttctcatc aacctgagac agactagcaa caatttcgac caactggtaa
      661 atatccagat aaacatcctt ataaaagagg tggtcccgac cttccatagc accacggata
      721 atatgcccct taaccgtatt tcctaaagga gaacgcaagc cgtcttgtga gtaacctgac
      781 tggccccgca catccatgga aacaacaccg taaccagcta cggtgaagcc cagcatgtcg
      841 gcccagtccc agccacgtcc catataacca tgaaaatgga agattagtgg aaccttctcc
      901 tcactctttg gaagaacaat gcgtgcatag acctttcctt ccttgcttcc ttccaatgtt
      961 aactcatagc acttgacttg aggaatgtgg aaatctcttt cctccaagtg gtaggatgga
     1021 agcgtggaaa catttttcac ttccccatcc cagaaagcat caaagtcttc cggaacctca
     1081 tcccttcctc tataggtctt aatttcttct agcaaagctg gatttttcat agcatgctcc
     1141 ttttattttg taatcgttac cacaactgta gcatatctat gtaatcaatg caagaaagaa
     1201 ggataaatag aaagtgaatt tagattcttt acttttagga tgatgggctg aaagtagttt
     1261 tagaaacgaa aaagagcatt tcgctctctc ttctgtttat actagttttt tgcttccttc
     1321 ttttggaaag tggtttggta atttacttta atcgttactg tcatatgaga gtcctcgttt
     1381 ctttttgatg actttagtat aagagccaaa cctaaaaact agcttaatac tcttcgaaaa
     1441 tctcttcaaa ccacgtcagc gtcgccttac cgtatatatg ttactgactt cgtcagttct
     1501 atctgccacc tcaaaacggt gttttgagct gacttcgtca gtttcatcta caacctcaaa
     1561 accatgtttt gagctgactt cgtcagtttc atccacaacc tcaaaacagt gttttgagca
     1621 acctgcggtt agcttcctag tttgctcttt gattttcatt gagtataagt tttccttaga
     1681 gaaagcttaa tccagatgtt ctttcttttc cagatgaagg gcaatcatgc gccactctgg
     1741 atcttcttgc caagcttcta tagaactaat gtagctagca acctttcttg cttcttctaa
     1801 aatcggaaaa tcttcgataa tatcagccac ttggaactct ggaagtcctg actgtctggt
     1861 tccaaaaatc tcaccagaac cacgcatttt caaatcttcc tccgcaagga caaatccatt
     1921 ggtcgtttct gtcatgatgc gcatgcggtc tttcccagaa tccgtcttgg gattggcaac
     1981 gagaacagcg taggactgct tgtccccccg accgacacga cctctaagct ggtgaagttg
     2041 actgagaccg aagcgatcgg catccatgat aatcatgaca gtcgcattgg gaacgttgac
     2101 cccaacctca ataaccgtcg tcgaaaccag aatatccgtc tttctctcct tgaaatcctg
     2161 catgatctgg tctttttcgt cactcttcat cctaccatgt agaagagcca cctctgcctt
     2221 gcctgcaaaa tgagtcgtca actcctctga taaggcaatg gcatttttca aatctagagc
     2281 ttctgattct tcaatcaaag gagagatgac ataggcttgg gaaccttttt gaatttcccc
     2341 ctctaaccaa gtcaagacct gaggtagttg ctcatgtttg atccagcgcg tcacaatagg
     2401 cttccgacct gctggcatct ggtcgataat ggaaacatcc atatctccaa aggctgtgat
     2461 ggcaagcgtc cgtggaatgg gagtcgccgt catcatgagg acatctggat tgtcaccttt
     2521 ttcccgtaaa atacgccttt gccctacacc aaaacggtgc tgctcatcga taataatcaa
     2581 accaagacga gcatactcca ccccatcttg tatcagagcg tgagttccta taatcaaatc
     2641 agcctcaccc ttggcaatgg tctccaagac ttctctcttt tctgcagctt tcaaggaacc
     2701 tgtcaagaga gccagtttca aattgggaaa aaggttctgt aaactctcaa agtgttgctc
     2761 tgcgaggatt tctgttggta ccattagggc agcctgataa cctgctgtca ctgccgcaaa
     2821 catggccaag ccagcgacta ccgtttttcc actccccaca tccccttgta ggagacgatt
     2881 catgtggtgg tcggacttca tatcagttaa aatttcctgc aaactctttt cctgagcttg
     2941 ggtcagggca aaaggaagac ttgctttaac tgctgtcact ttttcctgag accaatccag
     3001 aaccagacca cttccctgaa ctctattttc agacttgagc atctgcagct gcatttggaa
     3061 ataaaagagt tcctcaaact ttatacggcg aagagcctgc ttgtattctg ccaaatcctt
     3121 tggaaaatgc atagctcgga ctgcctgaca acgggacatg agtttgtatt tgtctagtaa
     3181 agactggggc agattttctt ctatcaagag gtccagtccc tgatcaaaag ccgtcttgat
     3241 gaccttaacc agactggcct gactgattcc ctgagccaga cgataaacag gctggaggtc
     3301 atcttctacc tgagccagaa ccttcatccc attcagacta gccttagcgc ggtcccattt
     3361 tccaaagaca gcaagggttg ctcccaactc tattttatca gccagatagg gctggttaaa
     3421 gaaattcacc gcaaaaacga cctctccctg cttgagacta aaacgcaggc gattgcgctt
     3481 gaaaccataa tactggacac tagcaggagt cactacctga ccagaaagaa ctgccttctc
     3541 accgtcttcc agctccagca cctgcttggt tttgaagtct tcataacgga aaggaaagta
     3601 gagcaagaga tcttgcaagt tttcaattcc tagtttggca tatttttctg ctgactttgg
     3661 tcccacacca ggcaagacat gcaagggttg atgtagattc atgctccact cctttctttt
     3721 ctaataatat tctctcggaa tacggtcgct gaggaggcaa accacctcat agttaatggt
     3781 tacgcggtag gtcgctacct gagttgcagt gatttcctta tccccattgg agccaatcaa
     3841 ggttaccttg gttccgagtg ggtaaggctt aggcaatcga atagtgattt ggtccatcga
     3901 aaccctgccg acaattgggc aagcttggcc atctaccaag acagagaaat tttgcatgtc
     3961 tcttgtccat ccatctgcat acccgattgg tacggtcgcg atgacttgct cgctatccgc
     4021 ttgataagtt gctccatagc ccatgcaagc tccagctgga actgtcttga catgaaccag
     4081 agcagactcc aaggtcaagg ccggtatcaa atcataaggc aaatccaaga ccgctccact
     4141 tggattgagg ccatacatgg catctcccat acgaaccgca ttgaaaatag tctctacatg
     4201 ccaaagagtc gttgcagaat tgctagcatg aaccagctct ggaacttcct tcatactagc
     4261 taaaatagtt ttaaaccgtt ctaactgggc attaaaatag tcatctgatt cctcatcagc
     4321 agtagcaaag tgggtaaaga ttccttcaac acgaacaccg tgttgttgga gcaaatcttg
     4381 agcctgctca acctcactgg cctctctaaa accaatccgt cccatccctg aatcaatctt
     4441 gaggtggact gtcaatccag ttaggtccac ttccttatct aagagtgctt gaatccactc
     4501 cagtccagcc actgtcaagg tgaagtcata ttctttagct agagcaacag cttcgatttc
     4561 agaaactcct aaaatgagga ttggcttgct gagtccagct tgtctgagtt caatggcttc
     4621 atcgatattg gaaacgcaaa agccatcaac atcatcttga attgccttgg caacggcaac
     4681 agctccatga ccataagcat tggccttgac cacagccaac ttgagcgttc cttgagggat
     4741 atgagccccc atttgctgaa tattttgtcg aatagctccc agatgaatca gagccttggt
     4801 tggtctatgt ggactagctt tcatgatttt cctccaaaat gacactggct gtcacaaact
     4861 gatcggtgtg gctgatagac agccaaatct ttcctgaaaa tggtgcctga ctaaaataag
     4921 gcgccccacg ttcattgttc aagacttcca aatcctgaaa accgagcttg ctaatgcccg
     4981 ttcccatagc cttggaaaag gcctccttag ccgaccagcg accagctaaa tattctattt
     5041 gcctgcgtcc tttgagactg gtgaagcgct ccatttcctg agcggtcagt acacgcttag
     5101 caaatccttc atgtcgtgta actgcgcttt ctatcgaagc caattcttcg atgtcaattc
     5161 cgtgtccaac tatcattctc atcaaaagga gttgagattc cccaactcca tccttttcac
     5221 ttattttgtc aaggtaacat aaatctcttc taccaaggcc tctgtatttt cccaacctag
     5281 gcaagggtca gtaatagagc aaccaaagat ctctggttgg ttttgacgac catctgctag
     5341 gtaagattca atcataaatc ctcgaaccgt ctttttaatt ttctcattcc aatcacgatt
     5401 ctgcaaggtc tggcgaacaa ttcgaatctg ctccatatat tgcttgcctg agttatcatg
     5461 gttggtgtca atgaggataa aaggattttc aagtcccatg gtttcatagc gttcaatggc
     5521 ttggagtagg ttttcatagt agtaattcgg catataattg ccatactcgt tgactgctcc
     5581 acggaggata acatgggcca aaggattacc tgatgtctca acttcctgcc catgataaag
     5641 gaaggtttgc ttgttttgag cagcatagat ggcgttaaac ataacaccca aatttcctga
     5701 ggttggattt ttcatcccta ctggtgcatc aatcccagaa gccacaaagc ggtgctcttg
     5761 gtcttccaca gaacgagctc caacggcatg gtagctgacc aagtcatcca ccaagatcag
     5821 atttgacgga taaagcatct catctgccgt tgtcaaacca gtctctgtaa tcacgcggta
     5881 gtgcaactgg cgcacagcct gcaagccgtt aatcaggctt ggagccttag aagtatctgg
     5941 ctggtgaacc aatcctttat agccgtctcc attggtacga ggcttagcag tatacacgcg
     6001 catgaccatg aaaatcttgt ccgctacctt cttttgcaag gcggataaac ggcgagcata
     6061 ttccaagacc gcctcttcat tatcagaaga gcaaggacca atcaccaaga gaatacggtc
     6121 atcttcccct gaaataatat ctgccaattc cctgtcacgg ctttccttga gtctcaaggc
     6181 ttctgcagac aattgggttt cagccttgat gacttccata tcgatttctt gacctttttc
     6241 aataaatgcc atcttattct cctaacgttt tgtaaatttc tctgacaaga gcttctgtgt
     6301 tatcccaacc caggcaaggg tctgtgatag acttaccaaa tacttctggc tcattttgtc
     6361 gaccatcttc cagataagac tcaatcataa aaccacgaac gaactgctta attttttcat
     6421 tccaagcacg gttaatcaag gtctggcgga caattcggat ctgttcaata tactgcttac
     6481 cagaattgtc atgattggta tcaatgatga taaaaggatt ttccaagccc attttctcat
     6541 actgggcaat ggtatcaatt aaattgtcat aatagtagtt gggaatattt tttccatact
     6601 cattaagagc accacgaaga atagcgtgtg aaagcgggtt cccagttgtt tctacttctt
     6661 ttcctaagaa aaggaaactt tgtttgtttt gagcagcata aatcccatta aacatgacat
     6721 tgagatttcc agaggttgga tttttaaaac cagtagaaaa tcctgcccca cttgccacaa
     6781 agcggtgttg ctggtcttca actgaacggg caccaactgc catgtaagaa atcaaatcat
     6841 ctacaagcgg aaggttttca ggataaagca tttcatcagc agttgtcatc cctgtttctg
     6901 tgatgacacg atagtgaaga tggcgaacgg ctttgattcc attgataaga ctaggcgctt
     6961 ctgtcgcgtt aggctggtga atcaagcctt tatagccatc tccgttggta cggggtttgg
     7021 cagtataaac acgcataacc ataaagatac gatctgccac ttcttcttgt aggactgcca
     7081 aacgcttagc gtattcaagg acagcttctt cgttatcaga tgagcatggc ccgattacca
     7141 agagaattcg ctggtcttct ccacgtataa tggcttctag ctcttgatct ctctgtgatt
     7201 tcctctccaa agcctgacct tctaattttg acaaggcacg aacttcttct atatttattt
     7261 tagagctttt tgctgtaaat accataactc atctccttat aaagcaaacg gatacccagt
     7321 aaaaatttac ttttcacaag atatccgcct ctaaactatc tcattttatt gtcttttacc
     7381 gtgacagttt ttaaatttct taccagaacc acatgggcaa agttcattgc gtccaatctg
     7441 gctcaaatcc aaatcttctg gcatacttgc ttggtgagca gcgatattgc gagtcgctgt
     7501 tgtactgata tgacgttctg cctgtggtct ttcttgttca tgaatttgtg ctttcatcat
     7561 caagcgtgtc acatcaaact caatcgaacc aatcatatca ttaaacatac ggaaaccttc
     7621 tgcctgatac tcaacaacag ggttgttctg agcatagcca cgaagtccaa ccgcgttacg
     7681 caattgatca agggcatcga tatgatctgt ccacttgtta tccaccactc gtagaatcaa
     7741 aactttttgg aattctttaa ctgcttcttc atcgcgtagt tttgaaacct gactatcgta
     7801 aaccttcaag gcacgttgga aaagctcttc cttgatggcc ttatcagaca agcctgacaa
     7861 gtcttccatc gtaatagaat cttcaggaag caagttgtac ttagcaaagt tcaaaattgc
     7921 ctctagtttt tcatcttgtt tggcacgcgc atgaccatca acgacacgtt caatcgtgcg
     7981 cttaatcata gactgaattt caggtgccaa gtcacgatct gcagtgatga catcgtaacg
     8041 ttgagcatag ataatctcac gttgttcacg catgacatca tcgtattgaa ggacttgttt
     8101 acgggtatcg tagttatttc cttcgacacg tttctgagct gcttcaacct gacgcgtcaa
     8161 catgcgagac tcaatcgcct cttcagacat gttcaagcgt tcaaagattc ccttcaagcg
     8221 ttcagaacca aaacgtttca tcaaatcatc ttcaagagat aggtagaatt gtgactcacc
     8281 tggatctcct tgacgacctg aacgtccacg aagctggtta tcgatacgac gactttcatg
     8341 acgttctgta ccaataacac aaagtcctcc aagttcacga acaccttcac caagcttgat
     8401 gtcggtacca cgacccgcca tgttggttgc gatggtaacg gcaccacgtt gaccagcatt
     8461 catgatgatt tgggcttctc tatagtggtt tttggcattc aagacttcgt gaggaacacc
     8521 agctgcaacc aatttcttag aaatgtagtc actagtttca accgctactg taccaaccaa
     8581 gacaggttga cccttttggt aacgagcctt aacgtcttcg acaaccgctt taaacttaga
     8641 ttctatactt gcataaagaa ggtctgagtg gtcaatacgt tgaacaggac ggtttgttgg
     8701 gattggaata acacgaatgt tgtagatttc acggaattct tcttcctcag tcttacctgt
     8761 acccgtcata ccagacaatt tcttgtacat acggaaaagg ttttggtacg tgattgaggc
     8821 agatgtcttg gtttcatcct ggattggcac accttctttg gcttcaatag cttggtgcaa
     8881 tccatcagaa taacgacgac cttccatggt acgacctgta aattggtcga caatcaagat
     8941 ttcttgctct tcgctcacca catagtcaat atcgagaagc atgatgtagt tggcacgaag
     9001 ggcgttatcg ataaagtggg tcaaagccac gttttcgatg tcatagaggt tttcaagttt
     9061 gaagtagctt tcagccctgt caatccctga atcagacaaa ccaatagtct tagactgcac
     9121 atcgatgatg tagtcatctt tgttcaaaga ttttacatag tggtctgcca tgtgatacaa
     9181 ctgactggtt tcaaccgcat tggcacctga tacaatcaaa ggtgtacgag cctcgtcaat
     9241 caagatagag tcaacctcat cgaccaaggc atagttaagc ggacgttgta ccatgttttc
     9301 agcgcgaacg accatgttgt cacgaaggta gtcaaatccg atttctgagt tagttgagta
     9361 agtaatatca cactcatagg cttctttttt ctccattgga gatttggtag ccaagttaat
     9421 ccctactgac aaaccaagcc aagagtacaa ttcacccatc tcagtcgcgt cacgttctga
     9481 caggtattca ttaaccgtaa ctacgtgaac ccctttacct gaaagggcat tgaggtatac
     9541 cggcatggtc gcagtcaagg ttttcccttc ccctgtacgc atctctggca cgtcaccatg
     9601 gtgaagaaca atccccccca tgacctgaac cttataaggg aagagaccta ggacacgttt
     9661 ggcaccttca cggacaaccg caaatgcttc gtaaagcaat gaatccagtg attctccatt
     9721 ttgataacgt tccttaaatt caactgtttt tgcttttagt tggtcgtcag tcaaagcagc
     9781 catttggtct tcgtatttga aaaccttgtc agccatcttt tccagacgac ggatttctcc
     9841 tttatcattt tcgataattg tttttaaaat attagccatg tttttcctta ctttaaattc
     9901 cgaatatttt agaatgttcc tttaatttta gcacaattac tgattatttt caaggaagaa
     9961 tccccatttt gaaaaggaaa aagaggttag ttttcaagct aacctctccg acttttatga
    10021 ctgtttaatt gccaaaaatc ggcaaaatgg caaatgtgat aaataagtta atccaaagag
    10081 aaagacagca aatcagtcca aaaacaaaga gactgacttt cttggacagc aaggccatca
    10141 aaatggacag aattccaaaa actagcaaga ctttctgtat gatgaagatg tcaatcctta
    10201 cccccagaat cgctctatcc caagggataa agaagaaaga cagccagatc agcagaacta
    10261 aaccaatata aaccttggaa tagcgtgtaa taaatgattt tagatgtgcc ataagccatc
    10321 tcctaggatt gtatatcctt tacatattgt atatgtccat tttcgacaac taatacatct
    10381 gtacaaaggt ctgaaatatc ttccgatatg tgcgacgtca ggataaccag accattttct
    10441 tttttcaggt aagataaaat gacctgtttg gttaacctca cacttttctc atccaaagca
    10501 ttcataggtt catcgagaaa gagtatagaa ggagaggaaa taaaggcttg aatcaaggcc
    10561 attttttgct ttgttcccaa ggataaatga cggtattcaa catcttcaaa ttcctgtaaa
    10621 tcatagtatt gaatccaata ggcaattctt ttttccgtaa ctttagatga aaaatacctt
    10681 aacagttcca aattttctct cagggataaa tgagataaaa actcgacttt ttcaattaaa
    10741 attcctgcat cctgaatata atgatttttt accccgtaaa ctttaccatc ttgaagaact
    10801 tttcctttgt caagcttaat ataaccagca agtatcttta aaagaaccgt cttgccagat
    10861 ccattatcac ctttaagtcc ataaattttt cctgattgaa acaccaaatt caactgattt
    10921 aaaataggtc ttttcccata aattttctgt ccatttataa tttcaagtct catcgtttat
    10981 cctttcttaa atttctcttc caagcgacta agaatcaaca taactaataa gcttaagagt
    11041 actaaataca ctgttacaat atgatccatc atccattctt gtcccacaaa tgccaaaaaa
    11101 tagagaaaaa aagaaaaacc agcgcccgca ctaatcagtg ctatcagata agacagtagt
    11161 aagtacaaca tcattaactg agcaagtaaa aagaaaagtg cgactgtttt gaaatgaatt
    11221 cctagcggaa gtaaaccaag aaaccagaca aacaaatctt gagtaaaaaa tttagaaatg
    11281 acaaccaatc gtttccttgt ataatcgaaa aagaaattaa
//
LOCUS       DAVLTM010000061        11218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLTM010000061 DAVLTM010000000
VERSION     DAVLTM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.062"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(102..701)
                     /locus_tag="VYN16_001368"
     CDS             complement(102..701)
                     /locus_tag="VYN16_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403888.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(838..1722)
                     /locus_tag="VYN16_001369"
     CDS             complement(838..1722)
                     /locus_tag="VYN16_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1403889.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1726..2112)
                     /locus_tag="VYN16_001370"
     CDS             complement(1726..2112)
                     /locus_tag="VYN16_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1403890.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2105..3448)
                     /locus_tag="VYN16_001371"
     CDS             complement(2105..3448)
                     /locus_tag="VYN16_001371"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1403891.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3535..4419)
                     /locus_tag="VYN16_001372"
     CDS             complement(3535..4419)
                     /locus_tag="VYN16_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403892.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4431..5048)
                     /gene="cysE"
                     /locus_tag="VYN16_001373"
     CDS             complement(4431..5048)
                     /gene="cysE"
                     /locus_tag="VYN16_001373"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1403893.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHSFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHAVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5064..7277)
                     /gene="pnp"
                     /locus_tag="VYN16_001374"
     CDS             complement(5064..7277)
                     /gene="pnp"
                     /locus_tag="VYN16_001374"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1403894.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7962..8828)
                     /gene="metF"
                     /locus_tag="VYN16_001375"
     CDS             complement(7962..8828)
                     /gene="metF"
                     /locus_tag="VYN16_001375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1403895.1"
                     /translation="MSCQTPSLSFEVFPPNPAVGNDNIILALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNEHFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8892..11141)
                     /gene="metE"
                     /locus_tag="VYN16_001376"
     CDS             complement(8892..11141)
                     /gene="metE"
                     /locus_tag="VYN16_001376"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1403896.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFNEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
BASE COUNT         3146 a         2523 c         2225 g         3324 t
ORIGIN      
        1 atgaaaaagt gttgtgatct ttattttgtt ttataataat agtgagaaag cctatcacta
       61 ctacaaatca cggggaggtg aataagtgag tggtacagcc actacctcgc atattttgtc
      121 acatcattta acggtacata ataagttgta ccatctgaat aagttgctac aatatcattt
      181 gcatgctctc cttcaccttt agcaaaggtt ggagctcctg ctggatgatt tttatttgcc
      241 tctttcaatt tttcaataat ggcatttttt tctgtatctt ttatattatc aggatttttc
      301 actaagattt tgtctggata tgtcggttta gcagaaacaa tttttactgt tacttctttt
      361 ttattcgaag cacttgtcca gtttccagca ttatctttag catttaattt tacagtaatt
      421 cctgaactag gaacttcagt agcaggttga ttatcaacat tattcaactt taatttcaaa
      481 agagctgttg catcagacgt tttatcaatc gttatatata atgatgaatt gttattataa
      541 ccagttcctt catatttagc tgtttgtgag ctacttgaaa cagaactgaa attataccca
      601 ctacctccct gattatcttc aatgcttacg tctaaatgaa cttccccact attatttggc
      661 ttagcaacaa ctgttatagt aaaataacat aaaatttgca taaatagatt agggaaatca
      721 aagcagcttc taggaatgtt ttagcagtca cagtgtactt tcccagcatc aagccactat
      781 aactctgcac ataaaaatgg agaagatggc aatcctcttc tccaaatatt aacttcttta
      841 caaaccaact atagttgaca aagaacctaa aatcaattga taacacaagg tcaggtcggt
      901 caactctttc aactgaagcc ctgtcaactc ttcccattta tcaatcttgt attggagaga
      961 attgcggtgc agatagagtt gctgggctgt tttagtgaga acagcactat tttcccaaag
     1021 agagagaatg atttcctgaa tctgatcttg atccaaaatc atctggtgta gacattcttt
     1081 aattggcttc aagtccacga gtctttctcc catactccaa agatagagct gagaaaaagt
     1141 atgaacacct tggtgaccct gacgccacca tgtcttgaac aaatcccgct cagctttgat
     1201 taagtctgat agggcttgat gtcctgtctg agaccaaacc tgacccaaca tgatagaaag
     1261 acgaagtcca aaatcatact caaccgcttc aatcgtatca cttaaaatat ctcttacaga
     1321 agtgtatttg tcttgttgaa gcacgaaaac ataatcctga gctccgacct gtagcactgt
     1381 ctgacaattc ggaaaaagag tccgcatcat atctagccaa gaagtcagat tttcctgctg
     1441 aaaataagaa agatggcaat aaaccaactg aatcttttta aaaacttgcg gtgcctgtcc
     1501 cttgccttca accagatagg aataccaagg gtttagcgaa cgagcctgct cctgctgggt
     1561 caaaagggca accaactgct tttcacgctc gctgagccca gcttcctcca gcaaaatcca
     1621 ctgctgagaa gctaaaggga gcgtgagata gccctctttc tctactggtt ggtctgaaat
     1681 ccgagcctca ggaaaccagt cttgtagttc tttttccctc atgttctagc cctccacttt
     1741 ttggatgcac catgaaacca aactctcaag acgttccaga ttctcagtca tatggagata
     1801 gcccataacc gcttcaaatc ccgtggacat acgataagtc acgacatctg catttttagc
     1861 ctttgtgtgg ctattggtat tgcggccacg tttgtagatt tcttcttctt tttccgttag
     1921 gacctgctcc tccaacatga gagcaatcag gcgagcctga gccttggctg acacatactt
     1981 ggttgcttct tgatggagtt tattgggttt ggtcatacct ttgaggatga ggtgacggcg
     2041 aatatacata gaatacaccg catccccctc aaaggctagc gcaatcccgt taatgagatt
     2101 gacatcaatc acgtgtccac ctcactccat ccttggtatc aaggagctta attccttgag
     2161 taaccaattg gtcacggatt tggtctgctg tcgcaaagtc acgattggca cgcgcctctt
     2221 ggcgtttttg aatcaagtct tcaatctctg catccaaaac ttcctcaaca aagacaattc
     2281 caaaaatttc taacatatct gcaagagctt gcttgacact tgcatcatag ttccctgagt
     2341 tgatccattt ggccatttca aagacaactg tgataccgtt ggcagcatta aaatcttcat
     2401 ccatagctgc tacaaactta tctttaaagt tttgtaactc ttgggcatcc acgtttcctg
     2461 taaatggttg ttcgtaagta ttcttcagat acttgagatt ggtctcggca tcgcgaactg
     2521 ccttttccgt gaagttgata ggcttacggt agtgctgggt cgcaaagaag aaacgaagta
     2581 cttgcccatc aagagtttta agggcatcgt gtaccgtaat gaagttaccc aaggacttag
     2641 acattttgac attgtcgata ttgacaaagc cattgtgcat ccagtagtta gcaaaagcct
     2701 tgcctgtttt agcttcagat tgggcaattt cattggtgtg gtgtggaaac tctaggtcag
     2761 ctccaccacc gtggatatca atggtatcac ctaaaatctc tgtcgacatg actgaacact
     2821 caatatgcca acccggacgt ccaggtcccc aaggactatc ccaagaaatc tcacctggtt
     2881 tggaagattt ccatagagca aagtctacag gattttcctt acgagccgtt tcttcatcgg
     2941 tacgacctga agcacctagc tccaaatctt ccaaggtttt attagccaat ttagcatagt
     3001 tgtgggattt ttctacacgg aaatagacat ccccttgact ctcataggca aagcctttct
     3061 cgatcaagtc ttccacaaaa cggatgatgt ctgccataaa ctccactaca cgcggatggc
     3121 gagtcgcagg tttcacgccc aaggccgtca catcctcacg aaaggcagcg atgtacttat
     3181 ccgcaacctc ctgaggcgtg atgccttctt ccctggcacg gttgataatc ttatcatcca
     3241 catctgtaaa attggaaata taggcaacct tatacccacg gtactcaaaa tagcgacgaa
     3301 tcgtatcaaa agctaccgtc gaacgggcgt ttcccacgtg gatatagttg tacaccgttg
     3361 gcccacaaac atacatcttg atcttgccgt cctcaatcgg gacaaattct cgcaaatcac
     3421 gagacatggt gtcatagatt ttaatcataa atcataatca ggaaagctaa aatccaagaa
     3481 ctgttagttt catcactaaa ctaagagctc aagtaaattt cagtccgaat gtctctacac
     3541 ttcggaatcc cttgctcctt tctcattcag ataaaccacc tgtgtctgtt tgacaaagcc
     3601 aattttttca tacaaacgtt tggcacctac attgctatct tccactgcaa tctgaaattc
     3661 cttatcattt tgctcaatta gttggttgac aagggatttt gctaagtagc ttccatagcc
     3721 tttcccacgt tcaggttctg atattgctaa accgtagaag taattcgtat cgctcgataa
     3781 atcaaccgtg caagtcccaa taacctgacc agcttttaat aaaatatata gtcggctttc
     3841 tggatctttc agagcttcag cgacatatct atccacaact tctctcgatt catgttcctc
     3901 tgaaaatgcc tgaaatttta attgactaat ttgatcctga tacgaactat ctgctaacaa
     3961 aatttcaaga tgggaaacat ttgctaacgg ataaggtctt ctatccttac ctaaccaagt
     4021 ttctgtctct tcatcctcga ttagtcccca gttactggca aagtcaggat gattctctaa
     4081 aaaaatacgt tctgtctgaa aagtgactga ccgaataggg aaagaagctg tttctctctc
     4141 aaaactagta aacaatgcac gcgcaatccc ctgacggcga tgacctggat gaaccagtat
     4201 cgtcacttct acatcttggt catctgcata gacagttaat aaaccaacaa gtttgccttt
     4261 ttcataataa aggaaaaagg cgggcatgtt tgggtcaaaa ttaagcatgt tagagagata
     4321 gggatcgcga taggtaccgt catagttttg gcaacagtta attacttttt tcgcctcaga
     4381 tagctcctct tggcttaact tgtttcttgc ttgaatcata taggtatcct ctacaaacca
     4441 gacgatctgt gactggcatc tttagcctgc tcgagtttat tgacataata ctctcgtttt
     4501 tcttcgactg cgtgaataac aggctcatct ttcttaccat gaagacggac aatcttggcc
     4561 ggaataccga caaccgtcac atcactaggt acatctgcta cgacaactgc tgcagcaccg
     4621 accttggcat tttcaccaat ttccacaggt ccgataactt gggcatgggc tgatatgagg
     4681 gctccctttc gtacagtcgg atggcgtttg ccacagtctt tccctgttcc cccaagagtc
     4741 actccgtgat agagaagaac gcctttttca acaatcgctg tctctccaat caccagacca
     4801 gaaccatggt caataaagac acctgaatca atctgggctc ctggatgaat ctcaatctga
     4861 gtccaaaagc gccaaaactg actgtgcata cgagctaaga gtttgaagct gtgcttccag
     4921 agaaaatgcg agagacggtg ggccgccaag gccttgacac ctggataagt cagcaaaacc
     4981 tccaaagtgg tgcgggccgc tggatcattt tcttttacga tatcaatggt ttcgcgccac
     5041 caccccatac atttctcctt ttcttattct gaatcttttg gtgtttctgt aaattctttc
     5101 ttaggtttgt gatccttgtg atgacgtggg cggtgagggc gctcagactt ttcacctttt
     5161 tcatcatgct caggttttgg cggacgaggt agaagagcct tcatagaagc atcgatacgg
     5221 cctttttcat caatcttgat aaccttaaca tcaacttcat ccccgatttc taccaaatcc
     5281 tctacacgat tggtacgagt ccaagccatc tcagagatat gaacaagggc atctgtctta
     5341 tcaaagaggt taacaaaggc accaaatttc tcgatacgaa cgactttagc acggtaaact
     5401 tcatccactt tggcttcacg aaccaaacca gcaataattt ctttggcacg gttaatagca
     5461 tcttggtcac tagagtagat agacacattt ccttcttcgt ctatatcaat cttaacacct
     5521 gtttcagcaa taatcttgtc gatggtttct ccacccttac cgatgacaat cttaatcttg
     5581 tccacatcaa tcttgatcgt atcaattttc ggagcagttg gagccaattc tggacgaact
     5641 tctggaatgg ttgcttcaat gacatcaagg atttcaaaac gcgctttctt ggcttgagca
     5701 agagcctccg tcaagatttc tgcagtaatc ccttgaatct tgatatccat ttgaagggct
     5761 gtaatcccat cacgagtacc tgcaaccttg aagtccatat ctccaaagtg atcttccaaa
     5821 ccttggatat ctgtcaatac tgtgtagtta tttccatctg agataagtcc catagcaata
     5881 ccagctactg gcgccttgat tggcacacca ccagccataa gggcaagagt tcccgcacag
     5941 atagaagctt gagatgaaga accgtttgat tccaaaactt ctgctactag acggatagcg
     6001 tatgggaatt cttccaagct tggcaagact tgagcaagag cacgctcacc aagagcaccg
     6061 tgaccgattt cacgacgacc tggcgcaccg taacgacctg tttcccctac agaatattga
     6121 gggaagttat agtggtgcat aaagcgtttc ttgtactctg gatccaaacc atcaatgatt
     6181 tgagtttctc ccatcggagc caaggtcaag actgaaagag cttgagtttg cccacgagta
     6241 aagagacctg aaccatgtac acgaggaagg aagtcaacaa ccgcatccaa aggacggatt
     6301 tcatcgacct tacgaccatc aggacgcacc ttgtcttctg taattaaacg tcgcacttct
     6361 gcgtgttcca tttgttccaa gatttcagcc acatcacgca taatacggtc aaattcttcg
     6421 tggtccgcat atttttcttc gtaaacggca gtcacttggt ctttcactgc ttgagttgca
     6481 gcttcacggg ccaatttctc ttctacttga actgcctttt ggaggtcact gttgtaggct
     6541 gcaatgattt cagcttgcaa ttcagcatcc acgtgaagca attccacttc tgctttttct
     6601 ttaccgacag cagcaacgat ttcttcttgg aaggcaatca attctttgac agcttcgtgc
     6661 cctttaagaa gcgcttccaa catgatttct tctgacaatt ctttggcacc agactctacc
     6721 atgttgatag cgtgcttggt tccagctact gtcaattcaa gaagagattg ctctgcttgt
     6781 tcttgacttg ggttgatgat gatttggcca tctacatatc ccacttgtac cccagcaatt
     6841 ggtccgtcaa atggaatatc tgaaatagac agtgccaaag atgaaccaaa catagcagcc
     6901 attggtgcag atgcattttc atcataagaa agcactgtat tgatgacttg gacttcatta
     6961 cggaaacctt ccgcaaacat aggacgaatc ggacggtcaa tcaaacgcgc tgtcaaggtc
     7021 gcatctgttg aaggacgtcc ttcacgtttc ataaagccac caggaaactt cccagccgca
     7081 tacatttttt cttcgtagtt gacttggagt gggaagaaat ccccagttgc cattttctta
     7141 gacataacgg cagcagtcaa gacagttgac tcaccgtaac gtacgacaac agatccattt
     7201 gcttgcttag caacctgacc agtctctaca attaactcac gacccgcaaa agtcgtttga
     7261 aacacttgtt ttgccatttt aatccccttt ggattgatga aattatacgc cttgcctaca
     7321 aagatcaaga taccaaggac gtcaaaagca aagtaaaaat aggaaactga cgaagtcttc
     7381 gatgaagaca agacagttta tcttttttac acagcttttc ggccgtgttc aattacacaa
     7441 gatattttgg acggttcggc ttgccgaaca tttctgtaga aaaataggaa ggtgacttcg
     7501 cactcgacga gtgctaggaa gcttatcttt tttcctaaga aatgagacca aaattcaagt
     7561 catcaagata ccaagccgtc aagcaactca aaggaagata ggaaatcgaa cgacggagcg
     7621 actgctccta gggagattta tctttttcca cagagttgta ggcaagttca gttttcaaga
     7681 tacatcatta gaaaggttta atactaaagt atctaaagct ttcacgctaa tcgctatcgg
     7741 gcgattagct aaatgcttta ctaactctct cgtcaaataa catcgatttg actcactcgt
     7801 gtcgttaaat cttacagttt aaatgcattg tattatttaa taccttcatc tttgtatcaa
     7861 gtacgtacag aatttatttt atcatatttt tcttaaaaag tgaggtcttt accattaaaa
     7921 aggaaccatt cccctcacct gagaagaatg gtttgctttt attatcctag agactggtga
     7981 ttaaacaagg catgggttgc ttgatggatg tattttgctg tatcagcatt attcatcgta
     8041 tagagatgca caccggcaac atcctgagtt accaagtcta cgatttggtc cactgcatag
     8101 gcaagtcctg ctgctctgag cgactcaggg tcatgctcat acttgtctaa gatggcttta
     8161 aatttgcgtg gaagatggat attctcacaa gtcttcaaga gtcggagagc ctgatttcga
     8221 ttcagaattg gcataattcc tgcatgaatg ggaacatcaa tcccagccaa gatgcacttg
     8281 tcctgaaaat catagaagtg ctcattgtca aagaagagct gagttacgag gctcgaacag
     8341 cctgcatcca ctttcttctt aagattttga atatctgaaa tctgatttgg cgaatctgga
     8401 tgcccttctg gatagcaagc tccaataata tcaaagtgag gggcttgttc cttgataagc
     8461 tcaatcaagt cggttgcata gcggaaatcc ttttgtggtt ccacgtctgg aataatatcc
     8521 ccacgaagag ccaagatttt ctgcacccca actttgtcca agtcagcaat agtttcagca
     8581 accttgtcct tagttagata aatagctggc aagtgggcaa tggtcggaat cgccaagtca
     8641 ttttggataa agtcagccaa acgaaccgtc gtttccttga tattaaattt attattgctg
     8701 gcagttacac tgataaaatg gggagccaac tcctgcatat cctgcaaggc taaaataatg
     8761 ttatcattac ccacggctgg gtttggaggg aacacttcaa atgagagtga cggtgtttgg
     8821 catgacatat gtaataacct tttctagttg atttcttttt gaacaaccac tgtatggaga
     8881 gaaatccaat cttacaattt ctcacgcgca gctttagctg cttcaacaag gcggatcaag
     8941 ctttcttttg tttctgggat accacgtgtt ttcaaaccac agtcagggtt gatccaaact
     9001 ttcttgcttg gcactttagc aaggatggct tcgattgtgt tgtcgatttc gccttcattt
     9061 ggtacacgag gtgagtggat atcgtaaacc ccaggtccca cttctgtttg gaagtttttc
     9121 gctttgagtt cgtccaagat ttcaaggttt gaacggctgg cttcaaagga aataacgtct
     9181 gcatccatgt tatcgatagc tgggatgata tctgtaaatt ctgagtaaca catgtgagtg
     9241 tggatttgtg tgtctgacgc tactgttgag tgtaccaagc ggaaggcagg aattgcccag
     9301 tcaaggtaat cttcgtacca gtcactacga cggagtggca atttttcacg aagagcagcc
     9361 tcatcaattt ggatgatttt cacaccagca gcttcaaggt caagcacttc atccttgata
     9421 gcaagggcga tttgaagagt agaatccttg atagagatgt cttcacgtgg gaatgaccag
     9481 ttgaggatgg taacaggtcc agtcaacata cctttaacag gtttgtttgt acgactttgt
     9541 gcatagctag accatttaac agtgataggg ttaagacgag tgacatcacc ccagatgatt
     9601 ggtggtttta ccccacgcat accgtatgat tgtacccatc catttttaga gaagaggtat
     9661 cctgacaagt tttgaccgaa gtactcaacc atgtcattac gctcaaattc accgtgaaca
     9721 aggacatcaa agtcaatatc ttcttgccac ttgatccatt cgtcaatcgt ttcagcaagg
     9781 aaagcgtcgt actctttttg agacaattca cctttacggt aagccaaacg tttggcacga
     9841 acttcttttg tttgagggaa tgaaccaatc gttgttgttg gaagagctgg aagtttaaaa
     9901 gcttcttctt ggatagcttc acgttctgca aaggctggca aacgagtgta gtctgcgtct
     9961 gtcaagccag cgatacgcgc acgaagttca gcattttcac caacacgctc agtcgcaaag
    10021 agttctttgt tggctgcaag agcttctgaa ccttgaccat tgcggatagc atccaagtca
    10081 cggatttcat ccaatttttc aactgcaaag gcaaagtggt tcaagagtgc tggttcaaat
    10141 tcttcattag cagttgtaaa tggcacatga agaagtgagc aagagcttgt caaaacgatg
    10201 ttttcagctg ggatttgctc aagaacagcc aagctctttt cgtagttgtt acgccagatg
    10261 tttttaccat tgacaatacc tgcatagaga gtcttgtcag ctgggaagcc acctttaacg
    10321 agttcaagag ttttcttacc ttcaacaaag tcaagaccga tagcatctac tggtaagttt
    10381 acaaggtcag cgtatacgtc acgaacatca ccgaaataag tttgaagcaa gacttcaaga
    10441 ccttttttgt cagccaagag tttgttgtaa aggttcaaga agagagcttt ttcttcagct
    10501 gtcaagtctt ttacaagagc cgcttcatcc aattggatgc gagtcgcacc aagttcagcc
    10561 aatttagcaa aaacttcttg gtaagcaact actaagctat ctacgaagtc gtctgctttc
    10621 acgccttctt caaagtctga caattgaagg aaagtgaagg gacctacaag aacaggacga
    10681 gtgttcaatc caagttcttt tgcttcttgg aactcattga aaatcttgtg accagccaat
    10741 tttacttgag tgtctttttc aaatttagga acgatgtagt ggtagttagt gttgaaccat
    10801 ttcttcattg gaagggcgcg aacgtcccct ttttctccct ggtaaccacg tcccaaagcg
    10861 aagtagcgct caaggtcaga caagtccaag ttttgaacgg atgcaggcac cacgttgaaa
    10921 aggaaagccg catctaggaa gttatcatag tgagaaaagt catttgatgg aatttcagtg
    10981 atgccttttt ctttgacaat gttccagtgt ttagcacgca agtcttttgc tgctgctaaa
    11041 agttcttctt ctgagatttc ttttctaaag tatttttcag ttgtaaattt taattcacgg
    11101 aattcgccca aacgagggaa accgatgatt gtagttgaca tgatgtgtcc tccaaaattt
    11161 gttgttgaaa ctatcttaac agaaaagaaa gcgtctgtat aattgtaaaa aattaggg
//
LOCUS       DAVLTM010000062        10993 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLTM010000062 DAVLTM010000000
VERSION     DAVLTM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.063"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            27..212
                     /gene="briC"
                     /locus_tag="VYN16_001377"
     CDS             27..212
                     /gene="briC"
                     /locus_tag="VYN16_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1403897.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            230..703
                     /locus_tag="VYN16_001378"
     CDS             230..703
                     /locus_tag="VYN16_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403898.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            716..952
                     /locus_tag="VYN16_001379"
     CDS             716..952
                     /locus_tag="VYN16_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1403899.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(1001..1423)
                     /gene="nusB"
                     /locus_tag="VYN16_001380"
     CDS             complement(1001..1423)
                     /gene="nusB"
                     /locus_tag="VYN16_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1403900.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1416..1805)
                     /locus_tag="VYN16_001381"
     CDS             complement(1416..1805)
                     /locus_tag="VYN16_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1403901.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1827..2387)
                     /gene="efp"
                     /locus_tag="VYN16_001382"
     CDS             complement(1827..2387)
                     /gene="efp"
                     /locus_tag="VYN16_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1403902.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2516..3958)
                     /gene="gatB"
                     /locus_tag="VYN16_001383"
     CDS             complement(2516..3958)
                     /gene="gatB"
                     /locus_tag="VYN16_001383"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1403903.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3958..5424)
                     /gene="gatA"
                     /locus_tag="VYN16_001384"
     CDS             complement(3958..5424)
                     /gene="gatA"
                     /locus_tag="VYN16_001384"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1403904.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLNSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(5424..5726)
                     /gene="gatC"
                     /locus_tag="VYN16_001385"
     CDS             complement(5424..5726)
                     /gene="gatC"
                     /locus_tag="VYN16_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1403905.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVVEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            5939..7483
                     /locus_tag="VYN16_001386"
     CDS             5939..7483
                     /locus_tag="VYN16_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1403906.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKEDERFASLEDGDKLFGSNPFYDQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7911..8109
                     /locus_tag="VYN16_001387"
                     /pseudo
     CDS             7911..8109
                     /locus_tag="VYN16_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            8220..8396
                     /locus_tag="VYN16_001388"
     CDS             8220..8396
                     /locus_tag="VYN16_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1403907.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            8512..8700
                     /locus_tag="VYN16_001389"
     CDS             8512..8700
                     /locus_tag="VYN16_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1403908.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            8857..9222
                     /locus_tag="VYN16_001390"
     CDS             8857..9222
                     /locus_tag="VYN16_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1403909.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            9225..10892
                     /locus_tag="VYN16_001391"
     CDS             9225..10892
                     /locus_tag="VYN16_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1403910.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         3172 a         2095 c         2481 g         3245 t
ORIGIN      
        1 cactatattt taagaaggta cttgcaatga caggtacaaa tacatttaca gttctttcaa
       61 ctgaggactt ggagcaaact tcaggtggtc ttgctgtttg ggaagatgga tatagtagat
      121 ggttatatta tagagaattt gctccctata tgaggcaagg ggcacttaat tcttatatag
      181 atgcttggaa gtacggcttc cgagcagggt aatctttcaa aaaagtacga tgagaaacac
      241 aaaaaaacta agacaatttg gaatattttt gcttattatt ttactatcta cctatttacc
      301 acaaacaatt ggattgtatg tgacgatcat tttagggtta ggagctgatg tctattctct
      361 tattttaaca atgggattag tgggaagttt ccttctatta atttggaggt taaaaaagaa
      421 aaagatgctc tttatctttg agaaaaagag ctggaactgg tcatttgtct tctacttatt
      481 tgcaacttat gtggtttatc agattctcgg taatttttgg gcacgctatg ctcatttgat
      541 taatcatagg aatattcatg atgagtattt taccgtgctt ttttcaaatg gacaaccaac
      601 ctttttatcg actatcttat cttttgtcct gcctgtaatc atcggtcctg tttttgagga
      661 gactcttgat agagggtatt ttatgaatac cttctttccc tagtccaagt actatttgga
      721 tgttatccta tctgctatca tctttgggat tagccatttg atactatctc acagagatcc
      781 aattagttta ttatattata gtctgattgg tttctttttt gcccttgttt atcgttctac
      841 cgacaatctg agattaacga ttctctgcca tagttttttt aactttctca atcatgcaaa
      901 acctatctgg atttttgttt acaattatat ctattatcat ttttttagat agtgggaggc
      961 aaagaaaaag tgtttgatat ctatcaaaca ctttttcttt ttattgttct tctgttacaa
     1021 actggctgag cagtccattg ataaaacggg cagatttttg atcggagaag tcctttgcaa
     1081 gctcgatagc ttcattaaca gcaaccagct gaggagtgtc aaatgaagtg atttcaaaga
     1141 ctcccaagcg aaggaggttt ctctccacga gcgttaagcg ttcaatggtc caacctgctt
     1201 ttaaatgctg agtgatttgc ttatctagtt cttccttttt agcttgaaca ccagaaacga
     1261 ggtctatcaa aaaggctgga agttgtacat ccgtatcttc acgatcatga gtataggcga
     1321 aacgacaagc agtttcgaca tccgtaccga actcaaggct catgagagct tgaaaagcgc
     1381 atttacggag ttggcgtcta gattctaata gtggactagt cattgaggaa gtcctcgtca
     1441 aatagatctt tcaattctgg ttttggtgtt ttatctggga cgatacctgc aacgtgaata
     1501 ttgatagcag cgagttctac atcagccata ttacggacgg catctttgac agctttctgg
     1561 atagcaaccg ctaccttagg aacttttact ccgtactcaa ggtagagata gatatctgct
     1621 gtgagttctt cgtccacgtt tttaagataa atgccacggc cgagtgaaag ttttgaaagg
     1681 gtatcagaca ctgatctgtt tgaaaaagag tgaacaccct ctacctttgc agtagcgata
     1741 gcaatgattt tttcaagtac acgtggggcg ataacgattt cgccaagttg ttcttcaatt
     1801 cccatagaat gacctctttc tagagattag gcacgagaaa cgtaagttcc ttctgcagtg
     1861 ttgataacga gtttttgtcc tgcttcgatg aagtctggaa cgtttacgac aagtccagtt
     1921 tccatcgttg ctggtttacc agaacctgta acagtagcac ctttgataga tggttgagtt
     1981 tcagcaactg tcaactcaac agtagtagga acggtgacac cgatcacttc agttccgtag
     2041 aattggattt tcacatcaga gttttcaagg atgtaaagca attcgttttc aacattgact
     2101 acagggattt cgtattggtc gtaagtttct gtattcatga agtatgctgt gtcatccatt
     2161 ttgtacaagt attgagctgg gacagtctcg ataatagctt gttcaaattt ttcctctgga
     2221 cggtagcttg tgtcaaatgt agaaccagta cggacatcac gcaatttcat acgcatgatc
     2281 gtgtttcctt tacctggttt gtggtgacta gcttccaaaa cgcgaatcaa tttgccgtca
     2341 gctgtttcaa aggtcatacc agcttttaat ttacttgctt caatcatgtt tttacctctt
     2401 taataaatat tattactctt cattttacca taaaatactc tggaaataaa gccaaaatag
     2461 ttattgggag ttggtagaga agaaaaaacc aaccttaggg ctggttttgt tctgtctagt
     2521 tttctttcaa cttcgccaat tcctgtgcaa gtagtttaag ggcaacttgt gggttggctt
     2581 ggcctttggt tgccttcata aggaatcctg taaaggcctt gtcggcgtta cgtttgcctg
     2641 acttgaagtc ggcaacagca gcttcgttat cggcaaagac ttggtggatg attgggatca
     2701 agatagctgg atctgaaatt tgaaccatac ctgctttttc cacgtattca cgcgcgccac
     2761 cgccattttt agctagatgg acaaagactt tcttggcaat cttagatgaa atagtaccgt
     2821 cttcgatgat ggtaatcatt tcaaccaagt tttctggtgt caattcgatt tgttccagtg
     2881 ttttaccttc agcattcaag aactgagcga cttccccttg gagccagtta gagacttgtt
     2941 tggcatcacc acctagggca acagcttttt caaagaagtc agaagtgact ttattagcag
     3001 tcaactgact agcatcgtag tctgataaac caaggtcaga tacataacgc gcacgacgtt
     3061 cttttggaaa ctctggcaac tcagtccgca tttcctcaat ccactcgtca gaaatttcaa
     3121 agaggggtag gtctggttct gggaagtagc ggtagtcagc agccccttcc ttgacacgca
     3181 tgaggatggt tgctttattc gcttcatcgt aacggcgtgt ttcttggcgg atttgaccac
     3241 ctgagcgaag aatttcagcc tggcgttgga cttcgtattc aagccccttg cgaacgtttg
     3301 agaaggagtt gaggttcttc aattcagtct tggtaccgaa tttctcttga ccataaggac
     3361 gaagggagat gttggcatcc acacgcatcg aaccttcctc catcttaacg tcagaaatgc
     3421 cagcatactg gataacttcc ttgagtgctg tcagataagc ataggcctcc tctggcgaac
     3481 gcatatcggc ttcagataca atctcaatca aaggtacccc ttggcggtta aggtcaacgt
     3541 aagagtagcc gtctgtaccg tgggtgtttt taccagcgtc ttcctctaag tgggcgcgtt
     3601 cgataccaat tttcttggtc gtaccgtctt ctagctcgac ttcaatccag ccgttataac
     3661 cgattggctc atcaaactga gaaatttggt aggctttggg attatcaggg tagaagtagt
     3721 tcttgcggtc aaagtgcatt tttttgtgga tgtccatgtt gagggcaaga gcagccttga
     3781 taccggcatc aacaacccct ttattgagaa ctggcagaac tcctgggaaa gaccagtcaa
     3841 tcacgttagt gttggcattt tgatcatttc cgaagtgggc agaagtaggt gagaagattt
     3901 ttgaattggt gttgagctct acgtggactt caagtccgat gactgtttca aagttcatta
     3961 gttgtcacct ccaaaaatca cgggttgttg tttgtggtag tctgttgttg cttcaaaagc
     4021 agcagcagct tggtaaatgg tttcctcaga gtacttggga ccaatcaatt ggagtccgac
     4081 aggtagacct tgagagaatc cagcaggaat cgaaattcca ggcagtcctg ccaagtttac
     4141 aggtatggtc aataggtcgg ctaagtacat ggcaactggg tcatggttga gagaattcaa
     4201 gtcataggca acacttggag cagttggacc caaaatcaaa tcataatccg cgaagacttt
     4261 ttcgaaatct tgaatgataa gtgtacggac ctgtccagcc ttcttgtagt aggcatcgta
     4321 gtaacctgat gaaagactga aagtacccag catgatacgg cgcttcactt cttcaccaaa
     4381 accttggcta cggctgttta catagatttc atcaagatta gtcgcatctt ctgcgcggta
     4441 gccgtaacgg ataccgtcaa aacgttgcaa gtttgatgaa gcttctgatg aagcgatgat
     4501 gtaataaacg gcaacaccgt atttagagtg aggaaggctg acttcttcga cgatagcacc
     4561 caatttttca aagtgtttgg ccgcgtttaa gattgtttcc ttaacctctg gatcaattcc
     4621 ttcgcctagg tattccttag gcaaagcaat tttcatgccc ttgatgtctt ggccgatttt
     4681 tgaagtaaag tcggcgatgc ggacaggagc agaagtagag tctttagcat cttcgctggc
     4741 aatagcgttg agtaagaggg cattttcctt aacagtagga gcaaaaggtc caatctggtc
     4801 taatgagcta ccaaaggcaa tgagaccgaa acgtgaaact gttccgtagg ttggtttgag
     4861 accaacgatt ccgttgaagg cagcaggttg gcggatggaa ccaccagtat cagaaccaag
     4921 tgacaagcga acttgtcctg aggctacagc tgcggcagaa ccacttgatg acccaccagg
     4981 aaccttgctg tggtcccaag cgtttttagt tgctccgtag tgtgaagttt cacctgaacc
     5041 acccatagca aattcgtcca tgttggtctt tccaacgaca atcatgccct tggtttttgc
     5101 attggcaaca gctgtcgcat caaagattgg ctcatagttg tagagcattt ttgaggcagc
     5161 agttgtgaga ataccgtctg tagagatgtt atccttaaca gcaagtggaa ttcctgaaag
     5221 gacattgtca gcatcaattc cagcttcatc aatggcttta gcttgaacaa gagcttgctc
     5281 ctcagcgatg gtgacaaatg aattgagggc ttcctcacga gacttgatat tttcaagtgt
     5341 tgcttgggtc aattctgttg cagaaatttc cttagagaca aggagattgt gcaactcttc
     5401 aatagtttta ttgttaaaag tcattaggca tctcctccat cgtctaggat agctggcacc
     5461 ttgatatagt agttgtcttg ttcaggtacg tttttaaaca agcgatcacg gtctgttcct
     5521 tcttcgacca catcagggcg gagtacagtc ttgcggtcag ccatagtcgt agtaggtgcg
     5581 acaccagttg tgtcaacttc gcccagcaat tcaaccatgt caacaatctt agacaaggtg
     5641 gtcgcaaagg cagcagtttc ttcttcagag aatcttaatt ttgaaagatt ggcaacgtgt
     5701 gttacctctt cttgcgtaat tttcatcttg catcctttcg tgaaatgatg atttttagtc
     5761 tgttctattt taccatattt tcctataaat aagggagggg gaagtgaaaa gaaatagaaa
     5821 ttgaaaaaaa tgctaaaatc cgatataatg gagtgttgaa aggaatggta aaatccaatg
     5881 taaaaatcat tctcagctat tgaaacaaga aaaatagaga atcaaagaaa gagaacttat
     5941 gaatattcaa gaagaaatta agaaacgtcg tacctttgcc attatctccc acccggacgc
     6001 ggggaaaaca accatcactg agcagttact ctactttggg ggtgagattc gtgaggctgg
     6061 tacggtaaaa gggaagaaaa cagggacttt tgctaaatct gactggatgg atatcgagaa
     6121 gcaacgtggg atttctgtta cttcatctgt tatgcaattt gactacgacg gcaagcgcgt
     6181 gaatatctta gacacgccag ggcacgagga cttctcagaa gatacctatc gtaccttgat
     6241 ggcggtggat gctgcggtca tggtcgtgga ctctgccaag gggatcgagg ctcaaaccaa
     6301 gaaattgttt gaggttgtga aacatcgtgg cattccagtc tttaccttta tgaacaagtt
     6361 ggatcgtgac ggtcgtgagc ctttggatct cttgcaagaa ttggaagaaa tcttgggcat
     6421 tgctagctac cctatgaact ggcctattgg gatggggaaa gcctttgagg gcttgtatga
     6481 cctctataac caacgtttag agctttacaa agaggatgag cgttttgcca gtctagaaga
     6541 cggggacaaa ctctttggta gcaatccttt ctacgatcaa gtcaaggatg acattgagct
     6601 tttaaatgaa gctgggaatg agttttcaga ggaagctatt ctggctggag aattgacgcc
     6661 tgtctttttc ggttcagccc tgacaaactt tggtgtgcag accttccttg aaaccttcct
     6721 caagtttgct ccagaaccac atggtcacaa gaaaacagac ggtgaaattg tggatcctta
     6781 tgacaaggat ttctcaggct ttgtctttaa aatccaagcc aacatggacc ctcgtcaccg
     6841 tgaccgtatt gcctttgtcc gtatcgtatc tggcgaattt gagcgcggca tgagtgttaa
     6901 tctccctcgt actggtaagg gggctaaact gtctaatgtt actcagttta tggcggaaag
     6961 tcgtgagaat gtgaccaatg ccgtggcagg tgatattatc ggggtttacg atacaggtac
     7021 ttatcaagtt ggggatacct tgacagttgg taaaaacaag tttgaatttg aaccactgcc
     7081 aacttttact cctgaaattt tcatgaaagt ttctgctaag aatgttatga agcaaaaatc
     7141 cttccacaag gggattgagc aattggtgca agaaggtgct attcagcttt ataaaaatta
     7201 ccaaacaggc gagtacatgc tgggagctgt tggtcaactc cagtttgaag tctttaaaca
     7261 ccgtatggaa ggcgaataca atgcggaagt ggtcatgagc ccaatgggta aaaagaccgt
     7321 tcgttggatc aagcctgagg acttggatga acggatgtcg tcaagtcgca atatcttagc
     7381 caaagaccgt tttgaccaac cagtctttct ctttgaaaat gactttgccc tccgctggtt
     7441 tgcggacaag tatccagacg tagagttaga ggagaagatg tgactcagta tcaacaattg
     7501 gaactaaagt tccaaattgt tggacgctag tcgctatttg gcgaactagc taactgcctc
     7561 actaactacg tttggcaaat agaatcgatt tgccaaactc cgtgtcgtaa tctaataatc
     7621 ggtaaccgct atggcggctt acctaaacgc ttcactagga aaaatccacg aatggtattg
     7681 atttgtggat ttttccgtcg tagcagaaag taggtagcct agctaactgc cttactaact
     7741 acgtttggca aatgaaatcg atttgtaaaa ctccgtgtcg taatgtaaaa attgtaaacc
     7801 gtaatgggac ttcatctaaa atctccactg gtatggtttt acgaacgaat gggcgaaaat
     7861 aatggttacg tagaaacata tgatgggaat aaaaagaaat gagataaaga gtgaaagaag
     7921 agaaaaaagc gattgtctta ggtgcagata atgcttatat ggataaagtt gaaacaacaa
     7981 taaaatctct ttgtgttcat ctattataat cttaagtttt atgtttttaa tgatgacctt
     8041 ccgcgagaat ggttccaatt gatggaaaaa agactagaga ctttgaatag tgagattgtg
     8101 aatgtttaga ttgacagtag tattctgaaa ggttatcgtc tcccctttga gggactttca
     8161 tatgcggctt ttttagatat tttattccaa agtatgtaag cgagtctcgt gtactgtatt
     8221 tggatttaga tattgttgta agaaaatcga tagacgagct gtgggatttg gacttgacgg
     8281 ctataccctt agctgcagtt agagatgatt tttatacaca taattttaat tctggtgttc
     8341 tgttaatcaa caatggcatg tggagagcag aaaatgtcac gcaagacctg atttaagaca
     8401 caaataaaaa atcttccaat tcccttgcaa aaaacaagaa agtgtgataa catagtacgg
     8461 tatgtggtgc tagcacatcc gctatattag atctaatagg aggaaaacac aatggctaaa
     8521 gtatgttact ttacaggtcg taagactgta tcaggaaaca accgttcaca cgcgatgaac
     8581 caaacaaaac gtgccgtaaa accaaacctt caaaaagtta ctgttcttat cgatggtaaa
     8641 cctaaaaaag tttgggcttc agctcgtgct ttgaaatcag gtaaagttga acgcgtttaa
     8701 taataaaagc aaaggagacc ttagggtctt ttttctttta ttgtgggtat aacatcgttt
     8761 gaaaaataga gtaaatatcc gctgatacat cattctgctt ttacacttgg gctgaaatat
     8821 gataaaatag agtatcaact agttgaggta aaaaggatga ctgtaaaaat taatacaaaa
     8881 gatggtcaaa tcgaactaac agatgaagtg attgcaaccg tcgtaggtgg agcagcaact
     8941 gagatttttg gtgtggtcgg tatggctagt aaaaatgccc tcaaagataa tttccaagcc
     9001 ctgctaggta aggaaaatta ttctaaaggt gtcgtcgtaa aggcagccga agatggcagt
     9061 attgcagttg atgtatatac cgtgttaagc tacggaacaa agattagtga agtgtcaaaa
     9121 aacattcaag agcgtgttgc ctttagcttg gaaaaccagc ttggaattac tgctcagact
     9181 gtaaatgtct acattcaaaa tatcaaagtt gtaggagaat aaccgtgtca aaaattacta
     9241 ctagcttatt tcaagaaatg gtgcaggctg catcaactcg cttgaataag caagctgaat
     9301 atgtcaattc attaaacgtc tttccagttc cagatggaga tactgggaca aatatgggaa
     9361 tgaccattga aaatggtgct aaagaagttg cagacaagcc agcttctaca gttggagaag
     9421 tagcgagcat tcttgccaaa gggcttttga tgggtgcgcg tgggaactca ggagtgatta
     9481 cgtctcagct tttccgtgga ttttcccaag ctatcaagga taaagacgag ttaacaggtc
     9541 aagacttggc tctggccttc caatcaggtg tggaagttgc ctataaggca gtgatgaaac
     9601 ctgttgaagg aacgattttg acagtttctc gtggagctgc tatcggtgct aagaaaaaag
     9661 ctgagcaaac agatgacact gttgaagtca tgcgcgcagc cttggaaggt gctaaaacag
     9721 ctctagctaa aacaccagac atgcttccag tattgaaaga agttggcgtt gtggactcag
     9781 gtggtcaagg attggtcttc atctacgaag gtttcctttc agcccttact ggcgaatata
     9841 ttgcatctga ggactttgta gcgactcctg ccaacatgag tgagatgatc aatgtagagc
     9901 atcataagtc tgtagctggt cacgtagcga ctgaggacat cacgtttggt tactgtactg
     9961 aaatcatggt agctcttaag caaggtccaa cctatgctaa agattttgac tacgatgaat
    10021 tccgtaacta cttggatgag cttggggatt ctctcctcgt tgttaacgat gatgaaattg
    10081 tcaaagtcca tgttcataca gaagatccag gacttgttat gcaagaaggt ctcaaatatg
    10141 gtagcttggt caaggtaaaa gttgacaata tgcgtaacca acacgaagca caggttgaga
    10201 aagaagctac tcaagttatc aagtcggctg aagaaaaaga gtatgcttta attgctgtgg
    10261 tggctggtaa aggtctagca gatatcttct gttctcaagg cgtggactat gttatcgaag
    10321 gcggtcaaac catgaaccct tcaacagaag actttatcaa ggctgttgaa caggtcaatg
    10381 cccgtaacat catcttcttg ccaaacaaca agaacatctt catggcagct caatctgcgg
    10441 cagaagtttt ggagcaacca gcagtagtgg tagaggctcg cactcttcct caaggtatga
    10501 caagtcttct tgcctttgat ccaagcaagt ccattgaaga aaaccaagag cgtatgacag
    10561 ctgctcttag cgatgtcgtt agcggaagcg tcacaacagc cgtgcgtgat acaacgatcg
    10621 atggcttaga aatccatgaa aacgataatc taggtatggt ggatggaaaa attcttgtgt
    10681 caaaccctga tatgcaccaa acattgactg aaaccttgaa acatatgttg gatgaagaca
    10741 gtgaaatcgt aaccttctat gtcggtgaag acggaagcga agaacttgcc aatgaaatcg
    10801 ctcaagaaat cgtagaagaa ttcgaagacg ttgaagtcga gattcaccaa ggtcaacaac
    10861 ctgtttaccc atacctattt agtgtggaat aagattaatg aaggactgag aaatcagttc
    10921 ttttttcttt tttagtaaat gaaatcggta tctttttaat aaaaacaaaa ataacattca
    10981 taaataaacg tta
//
LOCUS       DAVLTM010000063        10923 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLTM010000063 DAVLTM010000000
VERSION     DAVLTM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.064"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            11..733
                     /locus_tag="VYN16_001392"
     CDS             11..733
                     /locus_tag="VYN16_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1403911.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VYN16_001393"
     CDS             730..1578
                     /locus_tag="VYN16_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1403912.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VYN16_001394"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VYN16_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1403913.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VYN16_001395"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VYN16_001395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1403914.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VYN16_001396"
     CDS             complement(3249..5957)
                     /locus_tag="VYN16_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1403915.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEDHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     IVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATDQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNVSAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPSTI
                     LFYPQVGWEVYVIPVVAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VYN16_001397"
     CDS             complement(5959..6660)
                     /locus_tag="VYN16_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1403916.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6871..9282)
                     /locus_tag="VYN16_001398"
     CDS             complement(6871..9282)
                     /locus_tag="VYN16_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1403917.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYETYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TDVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGNGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9335..10027)
                     /locus_tag="VYN16_001399"
     CDS             complement(9335..10027)
                     /locus_tag="VYN16_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1403918.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10562..10918)
                     /locus_tag="VYN16_001400"
     CDS             complement(10562..10918)
                     /locus_tag="VYN16_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403919.1"
                     /translation="MSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILAII
                     GGSLFLATLKKFKSEE"
BASE COUNT         3187 a         2505 c         2052 g         3179 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gttgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat attctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtt ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 cgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctttgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcac
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctaccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgaag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctaccaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagtgtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcaaattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaaaca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgatcggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcggcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaaccaac tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggtgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacta tggatgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa agtttgttca aggtcttcct gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ctgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgat cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatgct agtactccaa actgtcaata tcctgaggat gctggttgag
     6001 caccacatcc ttgacactgg catcgtgcat ttgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cgctcccttt tgacgagaca tgtcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 gagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tattgagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 tccaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaataa aattgaacaa gatcaccttc
     6901 tgctgttgcc accgaaatac aatctttccc catgcatttc gcttttattt tttcttgatt
     6961 cattttaatc acactcttct caatatctgg ataagaaact cgcaaatctc ctccactcct
     7021 tgataaaatg gtcaactgta agagtttttt atcttcccag ctcatgctca cttcaaaatg
     7081 tccacgtgcc attaagcctg aaacagaacc tgttgaccaa gcatcaggta gggcagctag
     7141 aggtaccaga taagctgcat gagactggag taacatttct gccatgccac tagtagcacc
     7201 aaaattacca tctatctgaa aaggaggatg gctacaccaa aggtttggca aggtggatgt
     7261 ctttaactgc tctgccaata atttatgggc tcgattgcca tctcccaaac gcgcccagag
     7321 attgatctta ttagccttgg accagcctgt gccgccattt ccacgatcat tgaggctagc
     7381 acgcgccgct tcaatatact cttgtccctt gtagctaaag agattgccag gatagagtcc
     7441 cactagatgg gaagcatgcc gatgctgggc ctccactttc tcattttgaa aatactgctc
     7501 ttcctcctca taccactccc tgattcgacc agattgagtg atttgcaaag gatttagtaa
     7561 atcagacttc tccttaacat cagtcaacaa gtcctcatcc agtcccaatt cctgagcagc
     7621 ctgaataaaa tcatgaaata actgccaaat cagagattgg tcataagtat tgccaatcga
     7681 aatcggccca tgttctgggg aataagacgg agaagacacc caacgctgcg cctgctgatc
     7741 cttatgtaaa aaggcattcc aaaaacgaac cgtttccctc aacatgggat aaattttctc
     7801 cctgagatag tcttggtccc tataaaatga ataggtttca taaacggttt gcatcatcca
     7861 cgcattggca gctggtgacc aaccccaata gtaatcccaa ccaggtgccg tccaaccaaa
     7921 gggagtcgct tgagtatgaa ccaaccaacc attctcctca cctttctgag agacgattcc
     7981 tgcatacttt acagccgcta gacgaccata gacacgcaaa tcatctacat agttgatgac
     8041 cggaaagacc gcctctagga gattggtaac ataggctggc caataattca tctgcagatt
     8101 gacatttaag tgatagtccg aattccaagg aggattgtcg actgcattcc agactccctg
     8161 taggttagct ggtagagcat ctgggcagtc tctggacgaa ctaatcaata aataccgtcc
     8221 atactggaag aacagctcct ccaaagcctg cccttcttgt ggcttataat tttttaacaa
     8281 atcatctgta gtggatgcgt caacatcagc ttccaaatcc aattgaacac gctggaataa
     8341 ggcttggtag tcctcgatat gccttgattt caattgggta tagccctttt ctttagctgt
     8401 gtccaccaag tctatcacct gttgctctaa atctagtttc ttgcgataat tgctagcagg
     8461 attttgggca aaatccgtct tagcggccaa gaagagattg gcataactgg ctcctgatat
     8521 ctgaacccta tctgaccaaa ctctaatatc tccatccgtt tcccaagcta gataactagc
     8581 aaaccgcaga tcattatcct taactcttcc cttcatcaag atatgagaat cagtaatatc
     8641 caacttacac tccttgtaat cagatttttc ctgctcatac tttccatcag aagccaaatc
     8701 acaggtcaag gatagttcta tagtaaaatc tagagtttcc aacccttcct tagtaaaaca
     8761 ttgaaccaag agatcatctg gaaaactcgc aaaagcttta cgttcaaatc gcgttccctt
     8821 atagacataa gaagtcgtcg caagtgcctt actaatattc agctgtctct gatagtccgt
     8881 cacctgagac aaagtcgtac cttgctggct gaactcaatg tgaatatccc caaaagacag
     8941 ataggtccca tattgactcg tttttggccc aattaggtgc tgctcagcca gttccttagc
     9001 cagattgtaa tctctcttct ccaaagcctg ccgaatctca gccaaaaaaa catactgatc
     9061 ctgaagattt ccaccctgat aatctgaact atcaggaagt ggacctccag accagagact
     9121 tttttcatta aattgaatcc gttcagcccc tataaggcca aatacttttg ctcctaaaga
     9181 accattgcct atcggtaaag cttcttcttc ccaacccatg taagtggttg aagctggttg
     9241 cttgtaggcc agtacataat cttgtttttt attccttatc atacttccat actaacataa
     9301 aaagcctaga aatcaattct agacttacaa ttttttattt tccaaggtag tattcagaaa
     9361 cgacgttcaa tttttcgtcg aattcgaata ccaatggtgg gaagttaggg atttccacgt
     9421 ccatgatctc gtcatctgac aaacctttga tgtgttttac aagggcacgg attgagttac
     9481 cgtgagctcc tacgaatacg tttttaccat ctttaagagc tggagcgatt ttatcttccc
     9541 agaatggaag agcacgttcc aaagtcactt tcaagttttc agcatctgga ataactgagt
     9601 cgtcaagtga agcgtaacga cggtctgtgt gagctgagtg ctcatcatca cggtccatgt
     9661 ttggaggcaa tacatcgtat gaacgacgcc agatgtgaac ttgctcatca ccaaattgtt
     9721 cagcagcttc agctttgttt ttaccagtca aaccaccgta gtgacgttcg ttcaagcgcc
     9781 atgatttttc aactggaacc cacaattggt cagaagcttc aagagccaag ttagttgttt
     9841 tgatagcacg tttcaatact gaagtgtaag cttggtcaaa tttgatacca gcttctttga
     9901 tcaatttacc agcgtcaatc gcttgttgtg tacctttttc agacaaatca acatcagccc
     9961 aaccagtgaa aaggttagct ttgttccatt cagactcacc gtggcgagca aaaaccaatt
    10021 ttaccattag atggattctc ctttaaattt tccgaggttt cccctcgttt atctattcta
    10081 ttttacacaa tttttcacaa aaaagctagt caaaaccaaa aaggaaagga ctgtttgaca
    10141 atgtttggca atcacattgt ttttggttgt ttctattcta tcttcattat ttaacctttt
    10201 tttctgcgca tcgaataatt tttgagttga ctcttttagt ttagtaaacg agtcattatt
    10261 ttccataact gtgcctttga aaccttcctc agttttagta aaataatcat caaaaatatt
    10321 tttataaaga tctatataat ctgcataagt gcgtattgat tcaatcgcat cgtctgttac
    10381 aaccggtgta tcgaaagtga ttgtttcttt tgaactattg gatgttgcta ctgtcttctc
    10441 atcattactt gttgaagtat tctttgaact ttatgttcca catgcaacta gaagagaaag
    10501 agataataga gctactccta taccgataaa cgtttttatt cttttcatgc taaaatacct
    10561 tttattcttc tgatttgaat ttcttcaatg ttgcaaggaa tagagatcct ccgataatag
    10621 caagaatccc cccaacccat cctaagaatg gaatgagact aacttcacca gaaacaatca
    10681 ttagtacaga cggagctgca cctacatgct tatctccttt atagtaaaca atagcaatga
    10741 taccaagaac aagatttgca atcttcaagg cattcaagag caaggcaaca cctgaaaaag
    10801 cccctgcagt agctcctcca ttaagagttg tagcagctgt atttaccgct gttcccaaca
    10861 agacaaaagg acaaataaga agtaaaattc ctcccacaag accgacgata ccactaatta
    10921 aag
//
LOCUS       DAVLTM010000064        10629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLTM010000064 DAVLTM010000000
VERSION     DAVLTM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.065"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(94..1416)
                     /locus_tag="VYN16_001401"
     CDS             complement(94..1416)
                     /locus_tag="VYN16_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1403920.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPVITALVLSVFVAIAVFGGLKSISKVSTTVVPFMTIIYI
                     LGTLIVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1507..1595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1759..4095)
                     /locus_tag="VYN16_001402"
     CDS             complement(1759..4095)
                     /locus_tag="VYN16_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1403921.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4199..4747)
                     /locus_tag="VYN16_001403"
     CDS             complement(4199..4747)
                     /locus_tag="VYN16_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1403922.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4744..5046)
                     /gene="zapA"
                     /locus_tag="VYN16_001404"
     CDS             complement(4744..5046)
                     /gene="zapA"
                     /locus_tag="VYN16_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1403923.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5133..6014
                     /locus_tag="VYN16_001405"
     CDS             5133..6014
                     /locus_tag="VYN16_001405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1403924.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            6018..6632
                     /gene="lepB"
                     /locus_tag="VYN16_001406"
     CDS             6018..6632
                     /gene="lepB"
                     /locus_tag="VYN16_001406"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1403925.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6712..6834
                     /locus_tag="VYN16_001407"
     CDS             6712..6834
                     /locus_tag="VYN16_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEV1403926.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            6911..9277
                     /locus_tag="VYN16_001408"
     CDS             6911..9277
                     /locus_tag="VYN16_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1403927.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYIFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILDAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9325..10608)
                     /locus_tag="VYN16_001409"
     CDS             complement(9325..10608)
                     /locus_tag="VYN16_001409"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1403928.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAQFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3025 a         2418 c         2178 g         3008 t
ORIGIN      
        1 tcacacttcc aatcacttgc ataatatata ttatatatca ggctgataaa aattatttat
       61 aggcaaaaaa atcacacgag ctgtgtgatt ccattatttg tcaaaatact ttttagtttc
      121 agcaataacg actggcgaca agaccaagag ggcaatcaag tttggcagag ccatcaaggc
      181 gttaacgata tctgcgataa tccagaccat atccaactcg ataaatcctc ctaacaagac
      241 catgagcaca aaaaccacac ggtagagcca gataaagcga accccaaaga ggaactcaaa
      301 acagcgttct ccgtaatagt tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa
      361 gatggtcaag agggcaggcc caaagtgtga aaagactgtt gagaaagctg actgagtcaa
      421 ggcaacccca ttcaagtcac cactccaaac tccagttacc aagatggtca aaccagttag
      481 agtacaaatg atgagggtat caataaaggt tcctgtcatg gaaatcaaac cttgctctac
      541 tggttcattt gtcttggcag ctgcagctgc aataggagca gaacccagac cagattcgtt
      601 tgagaacaca ccacgcgcca caccattttg aatagccatc cgaacgctag caccagcaaa
      661 tccacctacc gcagcaaggg gactaaaagc tgaggtaaag actaaagcga ttgtgccagg
      721 gatttttccg atattaaaga aaataactat aagagttcct aagatataaa tgatggtcat
      781 aaaaggaaca acagtagttg aaaccttaga aatagacttg agtccaccaa agactgcaat
      841 cgctacaaag acagacaaga cgagagctgt gatgactggc gaaatcgtcg ttgtattttg
      901 gatagattct gtaatcgagt tgacttgggt gaaggttccg attcccaaga gagcaaccaa
      961 tactcctgct actgcaaaca aaacagcaag tggtcgccac ttttctccca tccctagaag
     1021 gatatagtgc atgggacctc ccgctactgc accatggtcg tccttggtgc ggtatttgat
     1081 ggccaagagt ccttccgcat acttggtagc cattccaaag aaagccgcca tccacatcca
     1141 aaatagagct cctggtccac caaccttgat agccgtcgca actcctatga tatttcctgt
     1201 tccaacagtt gatgccaagg ctgtacacag agctgcaaaa ctggatacat caccatgtcc
     1261 cttatcctgg ataaaaataa gctgaaaggc cttgggcaga cgcaaaacct gcaagagtcc
     1321 tagccgcatg gttaggtaaa tccctgttcc gaccaataaa atcaagaggg gcggtcccca
     1381 agcaaaatca tcgattgatt taagcaattc taacatttcc ttctcctatc gtttcaaccc
     1441 caaaagaaag agcacatgca agatacatgt actctggaat gcttagataa atgctaaaaa
     1501 gcggtctatc ctagctctgt ccttttacct gagagtttga gcagttgcct gccttgcccc
     1561 ttcggtgcct ttacggtctc tccagagttc cgtccattta cagtcatgga aaatcaaacg
     1621 attccccact tctattaaac ttcattcggt gttggtattt aattgattct aattttacaa
     1681 aaaatgttgg cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag tttttacttt
     1741 ataaagtcca gaatactgct atcctttaaa agtgacaata gtcgcaccac tgcctccagc
     1801 attttgtggg gcatagccga aactcttgac atgtttgttt ctttgcaagt atttggtaac
     1861 tccttcacgg atgactcctg ttccgatacc atggatgata tcaacttgag ccatattgtt
     1921 aagcaaggct tggtcgatga aggtatctag ctcattcatg gcttcttcat agcgcttgcc
     1981 tcgaagatcc agtctagctt gaggtcctcg cccagaagtt cgtttcacaa cattgacctg
     2041 tttcttcttg actggttttt cttgctgggc ttgaacaaga tcaaactctt tctcttccaa
     2101 ggtcatctta atcaagccaa cttgggcttc ccagcggccg tccttgagtt gactggtcaa
     2161 ggtaccacgc tgaccataac tgagaaccac gatatcatct cccacctttg gagctcgttt
     2221 tttcttggcc ttttgaagga ccttgttttt agacaagtcc actttttcag gagccaattt
     2281 tttcaacttg gccttggctt caatgatttc gtggggcttg agttgggatt tactgtggag
     2341 atttttgaga atctggtcac tttcacttag ggccatatcc acaatctcag cagcctgttc
     2401 acgcgccttg ttaagctcgg tttccttttc acgattaagc tcgttgtaga gtttttttag
     2461 cgcacggttc atcttgagat tttcttgctc cacctcacgg atattgtcca aacgtttgcg
     2521 gctttccagc gtctgctctt ctaattgctc aatgatacga ttgacgtcat tgtcctgatc
     2581 gatctgctga ctggcatctc ctacgataac ttcagatagg cctagacgtt tggcaatttc
     2641 aaaggcatta cttcggccag gaacaccctg cataaagcga taggttgggc gaagagttgc
     2701 agtatcaaac tccatactgg cattttgcac aaaggctgtc tcaataccgt aggccttgag
     2761 ttctggatag tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt cctccagaat
     2821 agccatggca agggctgctc cctcttgggg atcagtacca gcccccaact catccaaaag
     2881 taagagtgaa tgttggttga ccttgccaag aatatccacg atattggtca tatggctaga
     2941 gaaggtagac aagctctgct caatagactg ctcatctcca atatcagcaa agatttcttc
     3001 aaaaatacca acacgacttc ccttgtctgc taaaatcggc aatcctgact gggccatgac
     3061 ctgtgtcaag cccagagttt tgagcatgat ggtcttccca cctgtattgg gacctgtaat
     3121 gacaatagct gttaaatctt gaccaaaata gacatcattt gcgacggcat ttttgaccaa
     3181 aggatggcag acatggagca gttgaatctc ttgattttct gacagctgag gcacgactgc
     3241 ttgtctttct tggataaatc gaaccttggc acgaatcaag tccagatgac cgataatcca
     3301 agcgtcatta gcaatctcag ccgcatgagg gcggacacgc tcagaaattt cttggagaat
     3361 gcgaagcatt tcatagcgct catctgctcg cagactagca atttcttcgc tcagtttgac
     3421 tacctcacgt ggttcgatat agacggtgtt tccactagca gaaatatcat gaacgacacc
     3481 tgcaatctta ttgcggtagg tgtttttgac tggtaaaacc tgacggccat ttctgctagc
     3541 aacaattcct tccgtcaaca tctgcgcttt ttgcttgagt aagtcttgta aaacatcgcg
     3601 cacctgactc tcgctatcat gtatttttcg acggattcgc gccaattctt cactggcaaa
     3661 attttcaatg aaacccgcat cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc
     3721 atgtaatttc tcaaaccaaa gggctaattc ttccaagctg acattttcca gattggcgta
     3781 aaaattttga agttctcggc tggcaagaag cacgcgtttc aagagtagga actcctcgat
     3841 attgagatcc gctcccatct ccaacctctt gcagactcct gcaatttcct tagttgagag
     3901 aatagtaaaa tgcggttgct cgacgaaaag agcctgcatt tctttcatct cagcaaaagc
     3961 ctgtttgatt ttatctgctt tggcagtcgg agccagctgt ctcaattgct ccaagccctg
     4021 ctcggtcaac aaatgaggct caaacaaggc cttgacctta tcgaactcta atgtttctaa
     4081 tattttctta ttcatcttta tttccttgtc tttgaatgtc taggtgtggt agctttttac
     4141 attctgatag attcgagtca aatctgtaaa caaagggcta ggaaaactcc tgcccttttt
     4201 atccgattaa atttgtcacc cagatttgtt tgagccaact ggttgttatc ggtatgctct
     4261 ggatgatgtg ttttgcgacg atactctttt caagaggatt ttgtataact gccatgggga
     4321 tggtcgccaa gattgtcaag gccatttgca agacaaataa ggtcaccaac atggacaaga
     4381 tacctgctga aacttggaac aacttaccac ccagtttttt gctaggaatc aagtgtaaga
     4441 gaagaccaag caaacgacca atactataga caatcccaaa tacaagcaag tagccgatac
     4501 ctgcataaaa gaccttatcc agctgaaaga gttgatccga tgggaaaaag aaagtcccct
     4561 gaccttcctg cgaatttgca taagggagca ataaatggaa ttgctctcca agccccttat
     4621 aaaactggcc agccataaaa gccgatgcca tggctgaaat caggtaataa acctgtaaga
     4681 gcaggcctct ccgatagccg atataaaatc cccaaaccaa gaccaataga agaaggaatg
     4741 aaatcataag gaatcctcaa tcttgctctg ttcttgctta caagtcacaa gcttgtgacg
     4801 gagttcttct agctcttgct ccttatcgtc aaattcaatc tcacggctga gctgagttga
     4861 taaacagttg actgccaaca aaagagcgat tgtttcatca tctgcgctag gcatttgttc
     4921 tttaattgct tggtattttt ctgtcgcaac cttagcgatt tcctccataa aaaggttatc
     4981 atgttcgctt gtcaaggtta acgttttttt cccgaatgta aatttgaatc gatttagatt
     5041 tgccataaaa ttcacctcac gatattatac caaaattcgc taattttgtc agtttttaca
     5101 aattttactg cttttgtggt acaatagaaa ctatggcaag tatcacactc acaccaagcg
     5161 aaaaggatat tcaggctttt cttgaacact atcaaaccag tctggctcct agcaagaatc
     5221 cctatatccg ctactttttg aaactacctc aagcaacggt ttctatctat acttctggaa
     5281 aaatcttgct tcagggtgaa ggggctgaaa aatacgccag tttctttggc tatcaagctg
     5341 tagagcaaac cagcggacaa aatcttcctt tgattgggac agatgaggtg ggaaatggtt
     5401 cctactttgg tgggcttgca gttgtggctg cctttgtcac acctgaccag cacgactttt
     5461 tacgaaaact cggtgtgggg gattctaaga ctctgaccga ccaaaagatc cgtcagattg
     5521 ctcctattct caaggaaaaa atccagcacc aggcactcct tctctcaccc agcaagtaca
     5581 acgaggtcat cggagaccgc tacaatgctg tttcggttaa ggttgccctc cataatcagg
     5641 ctatctatct cctccttcaa aaaggtgttc agcctgagaa aattgtgatt gatgccttta
     5701 ccagtgctaa aaattatgac aagtacttgg cacaagaggc caatcgtttc agcaatccta
     5761 tcagcttaga agaaaaggct gagggcaaat acttgtctgt cgcagtttct tctgtcattg
     5821 cgcgtgatct ctttctggaa aatcttgaaa atctgggacg agaactgggt tatcagcttc
     5881 caagtggagc tggaacggct tctgacaagg tggctagcca gattttgcaa gcctatggta
     5941 tgcagggact caacttctgc gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac
     6001 gcttagaaag gtaagttatg aattcattta aaaatttctt aaaagagtgg ggactgttcc
     6061 tcctaattct gtcattacta gctttaagtc gtatcttttt ttggagcaat gttcgcgtag
     6121 aaggacattc catggatccg accctagcgg atggcgaaat tctcttcgtt gtaaaacacc
     6181 ttcctattga ccgttttgat atcgtggtgg cccatgagga agatggcaat aaggacattg
     6241 tcaaacgtgt catcggaatg cctggcgata ccatccgtta cgaaaatgat aaactttaca
     6301 tcaatgacaa agaaacggac gagccttatc tagcagacta tatcaaacgc ttcaaggatg
     6361 acaaactcca aagcacttac tcaggcaagg gctttgaagg aaataaagga actttcttta
     6421 gaagtatcgc tcaaaaagcc caagccttca cagttgatgt caactacaac accaacttta
     6481 gctttactgt tccagaagga gaataccttc tcctcggaga tgaccgcttg gtttcgagcg
     6541 acagccgcca cgtaggtacc ttcaaagcaa aagatatcac aggggaagct aaattccgct
     6601 tctggccaat cacccgtatc ggaacatttt aagagaccta agaggccgag aatcacaaat
     6661 ctcagcctct tcttctatcg tgagaagtga ttgtttattg acaaatactc aatgaaaatc
     6721 aaagaacaaa ctaggaagct agccgtaggc tgcttaaaac agtgttttga ggttgtggat
     6781 agaactgacg aagtcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtggt
     6841 ttgaagatat tttcgaagag tataaaataa aaacaatccc aactctcacc tattcatgca
     6901 aaggaatttt atggaagttt atttttcagg aaccattgaa cggattattt ttgaaaatcc
     6961 cagcaatttt tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga
     7021 tgattttgaa atcattgtca caggaaccat ggctgatgta attgagggcg aagactatat
     7081 tttttggggg caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta
     7141 tgatcgcgca aaaccaacta gtaagggatt ggtcaagtac ttttcaagta gccatttcaa
     7201 ggggattggt ctcaagacag ctcagaaaat cgtagatacc tatggcgaaa ataccattga
     7261 tgaaattttg caacacccag aaaagttaga aggcatcgca ggactctctg ccaaaaatcg
     7321 cgaggctttc gtctccactc tccgtctcaa ctacggaacg gagatggttt tggccaaact
     7381 agccaactac ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga
     7441 aacccttgat gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg
     7501 ctttacaatt gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg
     7561 cttccgcgcc ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta
     7621 tgttgaagca cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt
     7681 ggaactagac cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt
     7741 tcagcagatt gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg
     7801 cagtcacttg gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat
     7861 tcaaaaacat atcactacta tcgaggaaga gctgggaatt gagtatgata acattcaaaa
     7921 acaggctatc tgtgacgcta tccagaacaa ggtctttatc ctgacaggtg ggcctggtac
     7981 tggtaagaca actgttatca atggaatcat cgctgtttat gcccttttag aaggacttga
     8041 cttcaggaag aaaaacaatc tgcccattct tcttgctgct ccaactggac gagccgctcg
     8101 ccgcatgaat gaattgacag gtttgcctag cgcgaccata caccgccact tgggaatgac
     8161 aggtgacgat gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga
     8221 tgaattctct atggtggata cttggctggc caatcaactc ttctccaata tctcttctaa
     8281 cagtaagatc ctcatcgtgg gtgacagtga tcagctacct tctgtcagtc ctggacaggt
     8341 tctagcggat ctgcttcata ttcctttgat tcctcagact cgcttggaaa aaatttatcg
     8401 acaaagcgaa gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc
     8461 agctgatttc acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat
     8521 tcctgccact attgaaaaaa tcttagatgc cgccctcaga aatggtattc ctgcccgtga
     8581 tatccaagtt ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct
     8641 catgcaagac ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg
     8701 ccactatcgt aagggagaca aggtcattca tttggttaac gatgctgaaa tcaatgtctt
     8761 taatggggat ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca
     8821 agacgagatt gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta
     8881 caagattcgc ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt
     8941 tgtcatccta ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac
     9001 agccattaca cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta
     9061 tgctacccaa cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct
     9121 actggagaat gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc
     9181 tgaacaatcc tacatcctaa ccgaagaaaa ctgggacagc attccagcca tgattgggat
     9241 tacagacaca gacctcaaag agatttttgg aaaatagtac atcagaaaac ccaccaattc
     9301 aggtgggttt tctgtttatt aagattattt tactgttgct gtgcttgtga tcaattcaac
     9361 agcttttttg atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg
     9421 tgcaacttcc atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc
     9481 tgaagcatca aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg
     9541 tgactcagct tctgcttggt attggttatg aaggtcttct tgagtagttc cagtgatttg
     9601 gaagtacatg tcagggttga tcccttgacg ttgcaagttc ccaaggaatt catttactga
     9661 acggtgaact tcttcgtgga tcatttcttc tggaagttct acgatttcag cgttttctac
     9721 agctgtatca attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc
     9781 caattctttg cggtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc
     9841 ttttgcaagt tcatcgtcaa gagctggaac ttctttagct tttacttcgt ggatagttgt
     9901 cacgaattga gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac
     9961 gataacatca acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat
    10021 gaattgacct gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac
    10081 accgtcgata gaaccaacga agtcgatcac aacagtgtcg ccgttttcag cagcagcttc
    10141 cttgataacc aattcagcca ggttgttgcg ttcgcgttcg atacgctctt caacatcagc
    10201 gtcagttact tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt
    10261 tacttcaggt tttgtaacga cttcagcagt gataacccag tcttgacctt tttccattga
    10321 agttacgtca atttttggtt gggcaaccac ttcaagacca gcttctttta cagctgcttc
    10381 ataagcgttt ggcaaaagag cgttcataac gtcttggtaa agtgattctt caccaaattt
    10441 tttatcgaag ataggacgtg gaaggtgacc tttacggaaa cctggaacat taagagattt
    10501 cttcactgac ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa
    10561 agtcaagaca ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct
    10621 ccttaaaat
//
LOCUS       DAVLTM010000065        10522 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLTM010000065 DAVLTM010000000
VERSION     DAVLTM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.066"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            826..2085
                     /locus_tag="VYN16_001410"
     CDS             826..2085
                     /locus_tag="VYN16_001410"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1403929.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2101..2652
                     /locus_tag="VYN16_001411"
     CDS             2101..2652
                     /locus_tag="VYN16_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403930.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2645..3922
                     /locus_tag="VYN16_001412"
     CDS             2645..3922
                     /locus_tag="VYN16_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1403931.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3938..4798
                     /locus_tag="VYN16_001413"
     CDS             3938..4798
                     /locus_tag="VYN16_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1403932.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5177..5961
                     /locus_tag="VYN16_001414"
                     /pseudo
     CDS             5177..5961
                     /locus_tag="VYN16_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6152..8443
                     /gene="pcrA"
                     /locus_tag="VYN16_001415"
     CDS             6152..8443
                     /gene="pcrA"
                     /locus_tag="VYN16_001415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1403933.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVVRT
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNTQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8486..9166
                     /gene="radC"
                     /locus_tag="VYN16_001416"
     CDS             8486..9166
                     /gene="radC"
                     /locus_tag="VYN16_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1403934.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9163..9852)
                     /locus_tag="VYN16_001417"
     CDS             complement(9163..9852)
                     /locus_tag="VYN16_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1403935.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(9870..10511)
                     /locus_tag="VYN16_001418"
     CDS             complement(9870..10511)
                     /locus_tag="VYN16_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1403936.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
BASE COUNT         3161 a         1886 c         2346 g         3129 t
ORIGIN      
        1 aactatattg ttataaattg atttgaattt caaaattaaa ttgtttgatt cttatttcaa
       61 tttgttatag tatatctgat gtcaaagttc tcggcgagtc aaatagcgat tcccaagcct
      121 gactatcgtg aggtagcgga ttaaaatggt ctggggatag accgttttaa gtctgacgct
      181 ggaaataaga attgtcagaa gaagggatag agaaatcgtg gctctacgaa caggaacgtg
      241 ataataaggc gtatatagcg gataagaggg cctcaaactc taaagtccaa aaaggtagtc
      301 gtaacctaat aaagagaaaa cgacagatag aagtgatcct gagtcacggt tatctttaaa
      361 atagattgtc tgataggacg gtatgtataa aacgcttctg tgaattgaga gaaggggaga
      421 agttcttgct aaaatttagt tgaacagccg tattccgata cttagataag agatctagtc
      481 ttagccccta ctcagtttta ggggataaaa aaggggcaat agcgattcga gaaagattat
      541 actcttcgaa aatctcttca aatcacgtca atatcgcctt gccgtatgtg taggatactg
      601 actacgtcag ttccatctac aacctcaaaa cagtgttttg agcaacctgc ggttagtttc
      661 ctagtttgat ctttgatttt cattgagtat tagtaattca gttactaact cgtcaactct
      721 gatttatcca ataaaattga aaaggatgga aaaaaggata aatttatgat atactttatt
      781 ttgaagacct tattagaaat cttgaaagag tattgaaaac ttagaatgag aaaaattgtt
      841 atcaatggtg gattaccact gcaaggtgaa atcactatta gtggtgctaa aaatagtgtc
      901 gttgccttaa ttccagctat tatcttggct gatgatgtgg tgactttgga ttgcgttcca
      961 gatatttcgg atgtagccag tcttgtcgaa atcatggaat tgatgggagc tactgttaag
     1021 cgttatgacg atgtattgga gattgaccca agaggtgttc aaaatattcc aatgccttat
     1081 ggtaaaatta acagtcttcg tgcatcttac tatttttatg ggagcctctt aggccgtttt
     1141 ggtgaagcga cagttggtct accgggagga tgtgatcttg gtcctcgtcc gattgactta
     1201 caccttaagg cgtttgaagc tatgggtgcc actgctagct acgagggaga taacatgaag
     1261 ttatctgcta aagatacagg acttcatggt gcaagtattt acatggatac ggttagtgtg
     1321 ggagcaacga ttaatacgat gattgctgcg gttaaagcaa atggtcgtac tattattgaa
     1381 aatgcagccc gtgaacctga gattattgat gtagctactc tcttgaataa tatgggtgcc
     1441 catatccgtg gggcaggaac taatatcatc attattgatg gtgttgaaag attacatggg
     1501 acacgtcatc aggtgattcc agaccgcatt gaagctggaa catatatatc tttagctgct
     1561 gcagttggta aaggaattcg tataaataat gttctttacg aacacctgga agggtttatt
     1621 gctaagttgg aagaaatggg agtgagaatg actgtatctg aagacagcat ttttgtcgag
     1681 gaacagtcta atttgaaagc aatcaatatt aagacagctc cttacccagg ctttgcaact
     1741 gatttgcaac aaccgcttac ccctctttta ctaagagcga atggtcgtgg tacaattgtc
     1801 gatacgattt acgaaaaacg tgtaaatcat gtttttgaac tagcaaagat ggatgcggat
     1861 atttcgacaa caaatggtca tattttgtac acgggtggac gtgatttacg tggggccagt
     1921 gttaaagcga ccgacttaag agctggggct gcactagtca ttgctgggct tatggctgaa
     1981 ggtaaaactg aaattaccaa tatcgagttt atcttacgtg gttattctga tattatcgaa
     2041 aaattacgta atttaggagc ggatattaga cttgttgagg attaaaccgt agaggtgttt
     2101 atgaatattt ggaccaaatt agcaatgttt tctttttttg aaacggatcg cttgtatttg
     2161 cgtcctttct tttttagtga tagtcaggac ttccgcgaga tagcttcaaa tccagaaaat
     2221 cttcaattta ttttcccaac gcaggcaagt ctggaagaaa gtcaatatgc actggccaat
     2281 tactttatga agtccccttt gggagtgtgg gcaatttgtg accagaaaaa tcaacaaatg
     2341 attggttcta ttaaatttga gaagttagat gaaatcaaaa aagaagctga gcttggctat
     2401 tttttgagaa aagatgcttg gtcgcaagga tttatgacag aggttgttag aaaaatttgt
     2461 cagctttctt ttgaggaatt tggcttaaaa caattatcta tcattaccca ccttgaaaat
     2521 gaagctagcc aaagagttgc tcttaagtct ggatttagtt tgttccgtca gtttaaggga
     2581 agtgatcgtt acacaagaaa aatgcgggat tatcttgaat ttcggtatgt aaaaggagag
     2641 ttcaatgagt aagcatcagg aaattctaag ctatttggag gaattaccag taggtaaaag
     2701 ggtcagtgtt cgtagcattt cgaatcatct aggagttagt gatggaacag cctatcgggc
     2761 tattaaagaa gctgaaaacc gtggaattgt ggagacccgt cctagaagtg gaacaattcg
     2821 tgttaaatcc cagaaagttg ctatagagag attaacattt gctgaaattg cagaagtgac
     2881 ttcttctgag gttctggctg ggcaagaagg tttagagaga gaatttagta agttttcaat
     2941 tggtgccatg actgaacaaa atatcttgtc ttaccttcat gatggggggc tcttgattgt
     3001 cggagaccga acccgtattc agttgctagc cttggaaaat gaaaatgcag ttctggttac
     3061 agggggattt caggttcatg atgatgtgct taaactggcc aatcaaaaag ggattcctgt
     3121 tctaagaagt aagcatgata cctttaccgt cgcgaccatg atcaataaag ccttgtcaaa
     3181 tgtccaaatc aagactgata ttctgacagt tgagaaactt tatcgcccta gtcatgagta
     3241 tggttttctg agagagacag atacagttaa agattatttg gacttggttc gtaagaatcg
     3301 tagcagccgt ttccctgtta tcaatcaaca tcaggtcgtt gttggcgttg taaccatgag
     3361 agacgctggt gataaatcac caagcacgac aattgataag gttatgtctc gtagtctatt
     3421 tttggttgga ttatcgacaa atattgccaa tgtgagtcaa cggatgatcg cagaagactt
     3481 tgaaatggta ccagttgttc gaagcaatca aactttgctt ggcgttgtga cgcgacgaga
     3541 tgtcatggag aagatgagcc gttctcaagt ttcggctcta ccaacttttt ctgagcagat
     3601 tggacaaaag ctctcttatc accatgatga agtagtcatt acagtggaac cctttatgct
     3661 agaaaaaaat ggagttttgg ctaatggtgt attggcagaa attctgaccc acatgaccca
     3721 agatttagtt gttaatagtg gtcgcaatct cattatcgag cagatgctaa tctacttttt
     3781 gcaggctgtt cagatagatg atatattgcg cattcaggca cggattattc atcatacgag
     3841 acggtcagct ataattgatt acgatattta tcatggtcac cagattgttt caaaagcaaa
     3901 tgtgactgtt aaaattaatt agaaactagg agaaaagatg ataacattaa aatcagctcg
     3961 tgaaatcgaa gctatggaca aggctggtga ttttctagca agtattcata taggcttacg
     4021 tgatttgatt aagccaggcg tagatatgtg ggaagttgaa gaatatgtcc gccgtcgttg
     4081 taaagaagaa aatttccttc cacttcagat tggggttgac ggtgccatga tggactatcc
     4141 ttatgctacc tgttgctctc ttaacgatga agtggctcac gctttccctc gtcattatat
     4201 cttgaaagat ggtgatttgc tcaaagttga tatggttttg ggaggtccca ttgctaaatc
     4261 tgacctgaat gtctcaaaat taaacttcaa caatgttgaa caaatgaaaa aatacactca
     4321 gagctattct ggtggtttag cagactcatg ttgggcttat gctgttggta caccgtccga
     4381 agaagtcaaa aacttgatgg atgtaaccaa agaagctatg tacaagggta ttgagcaagc
     4441 tgttgttgga aatcgtatcg gtgatatcgg tgcggctatt caagaatacg ctgaaagtcg
     4501 tggttacggt gtagtgcgtg atttggttgg tcatggtgtt ggcccaacta tgcacgaaga
     4561 accaatggtt cctaactatg gtattgcagg tcgtggactc cgtcttcgtg aaggaatggt
     4621 cttaaccatt gaaccaatga tcaatacagg cgattgggaa attgatacag atatgaaaac
     4681 tggttgggcg cataagacca ttgacggtgg attgtcatgt cagtatgaac accaatttgt
     4741 cattacgaaa gatggacctg ttatcttgac tagccaaggt gaagaaggaa cttattaata
     4801 aaaagtgaaa agactactgg aagtttatta tgaaaaaatc cagtagatct tttcataata
     4861 aaacgcattg tatcaagtgt taggggctga tatcatgcgt ttttctgctt ttaagatttt
     4921 ttccaactct gtttgtaagc gcatcataac aaagggtcta ggattcaggg ctctcctcct
     4981 atatactatt agtaaagtaa aactaaggga ggatatttta gtgtcgcagt ctattgttcc
     5041 tgtagagatt ccacaatatt gtcgttttga ttctaaaaag agaaatggaa ttctgtttaa
     5101 tgttcgtatt gccaatctta aatttacctt ttttagatta gacttcctgc gaaacaaaat
     5161 atggtatagt agttctatga atgatgaagc aagtaaacaa ctaactgatg cacgatttaa
     5221 gcgtcttgtt ggtgttcagc gtaccacttt tgaagagatg ttagctgtat taaaacagct
     5281 tatcaactta aacacgcaaa aggtggacga aaacctaaat taagcctgga agaccttctt
     5341 atggccactc ttcaatatgt gcgagaatat cgcacttatg aacaaattgc ggctgatttt
     5401 ggtattcacg aaagcaactt actccgtcgg agccaatggg ttgaagtaac tcttgttcaa
     5461 agcggtttta cgatttcaag agctcctctc agttctgagg acacggtaat gattgatgcg
     5521 acggaagtac aaatcaatcg ccctaaaaaa acaattagcg aatgattctg gtaaaaagaa
     5581 atttcatgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg tttctattgt
     5641 catgatatga agttgttcaa aatgagtcgc ggaaatatcg gacaagctgg taaaatcttg
     5701 gctgacagtg gttatcaagg gctcatgaag atatatcctc aagcacaaac tccacgtaaa
     5761 tccagcaaac tcaagtcgct aatagctgaa gataaagcct ataaccatgc gctatctaag
     5821 gagagaagca tggttgagaa catctttgcc aaagtagaaa cgtttaaaat gttttcaaca
     5881 acctatcgaa atcatcgtaa atgcttcgga ttacgaatga atttgattgc tggtattatc
     5941 aatcatgaac taggattcta gttttgcagg aagtctattg aggtattgag ctagtttatg
     6001 aaaaaattga gtgaaaagtc gagtgtttta gaaacccaca gtgtagtatt ctagtttcaa
     6061 tccactatat tttgctactc cccgtaaagt ttctattttc cctgatttct gatataatag
     6121 aaatattgac ttcaagagta aggaagagaa gatgaacgca ttattaaatg gaatgaatga
     6181 ccgtcaggct gaggcggtgc aaacgacaga aggtcccttg ctaatcatgg caggggctgg
     6241 ttctggaaag actcgtgttt taacccaccg tatcgcttat ttgattgatg aaaagctggt
     6301 caatccttgg aatatcttgg ccattacctt taccaacaag gctgcgcgtg agatgaaaga
     6361 gcgtgcttat agcctcaatc cagctactca ggactgtctg attgcgacct tccactccat
     6421 gtgtgtgcgt attttgcgtc gcgatgcgga ccatattggc tacaatcgta attttacaat
     6481 tgtggatcct ggtgaacagc gaacgctcat gaaacgtatt ctcaaacagt tgaacttgga
     6541 tcctaaaaaa tggaatgaac gaactatttt ggggaccatt tccaatgcta agaatgattt
     6601 gattgatgat gttgcttatg ctgcccaagc tggcgatatg tatacgcaaa ttgtggccca
     6661 gtgttataca acctatcaaa aagaacttcg tcagtctgaa tccgttgact ttgatgattt
     6721 gattatgctg accttgcgtc tctttgatca aaatcctgat gttttgacct actaccagca
     6781 aaaattccaa tacatccacg ttgatgagta ccaagatacc aaccacgctc agtaccaatt
     6841 ggtcaaactc ttggcttccc gttttaaaaa tatctgtgtg gttggggatg cggaccagtc
     6901 tatctacggt tggcgtggtg ctgatatgca gaatatcttg gactttgaaa aggattaccc
     6961 caaagccaag gttgttttgt tggaggaaaa ttaccgctca accaaaacca ttctccaagc
     7021 ggccaacgag gttattaaaa ataataaaaa tcgccgtcct aaaaatctct ggactcaaaa
     7081 cgctgatggg gagcaaatcg tttactatcg tgccgatgat gagctggatg aggctgtatt
     7141 tgtagtcaga accatcgatg aacttagtcg cagtcaaaac ttccttcata aggattttgc
     7201 agttctctat cggactaatg cccagtcccg tacaattgag gaagccctgc tcaagtctaa
     7261 cattccttat accatggttg gtggaaccaa attctacagc cgtaaggaaa ttcgcgatat
     7321 tattgcttat ctcaacctta ttgctaattt gagtgacaat attagttttg agcgtattat
     7381 caacgagcct aaacgtggaa ttggtctagg tacagttgag aaaatccgtg attttgcaaa
     7441 tttgcaaaat atgtctatgc tggatgcttc tgctaatatt atgttgtctg gtatcaaggg
     7501 taaggcagcc caatctatct gggattttgc caatatgatg cttgatttgc gggagcagct
     7561 agaccactta agcattacag agttggttga gtccgtccta gaaaaaacag gttatgtcga
     7621 tattcttaac acccaagcga ctctagaaag caaggcaagg gttgaaaata tcgaagagtt
     7681 tctttctgtt acgaagaact ttgatgacac cactgatgtg acagaagagg aaactggtct
     7741 ggacaaactg agtcgtttct taaatgactt ggctttgatt gccgacacag attcaggtag
     7801 tcaggaaaca tcagaagtga ccttgatgac cctgcatgct gccaaaggtc tcgaatttcc
     7861 agttgtcttt ttgattggga tggaagaaaa tgtctttcca cttagtcgtg cgactgaaga
     7921 tccagatgaa ttagaagaag agcgccgtct agcctatgta ggtatcacgc gtgcagagaa
     7981 aattctctat ctgaccaatg ccaactcacg cttgcttttt ggtcgtacca attataaccg
     8041 tccgactcgt tttattaacg aaatcagttc agacttgctt gagtatcaag gtctggctcg
     8101 tcctgcaaat acaagcttta aggcatcata tagcagtggt agtatttcct ttggtcaagg
     8161 tatgagtttg gctcaggctc ttcaagaccg taaacgcggt gctgccccaa aatcaatcca
     8221 gtcaagcggt cttccatttg gtcaatttac agctggcgca aaaccagcat ctagcgaggc
     8281 aaattggtcc attggtgaca ttgctctcca caagaaatgg ggagagggaa ctgttctgga
     8341 agtttcaggt agcggtgcta ggcaggaatt gaaaatcaat ttcccagaag taggtttgaa
     8401 aaaactttta gccagtgtgg ctccaattga gaaaaaaatc taattttcca tccttctcac
     8461 gaataataaa gtgaggagga tttttatgta cagtatttca ttccaagaag attcactatt
     8521 accaagagaa aggctggcca aggaaggagt tgaagcgctt agtaaccaag agttgctagc
     8581 tattttactc aggacaggaa cacgtcaagc tagcgttttt gaaattgccc aaaaagtctt
     8641 gaacaatctt tcaagcctaa cggatttgaa aaaaatgacc ctgcaggaat tgcagagttt
     8701 gtctggtatt gggcgtgtta aggccataga attacaagct atgattgaac tggggcatcg
     8761 tattcacaaa cacgagactc ttgaaatgga aagtattctc agcagtcaaa agttggccaa
     8821 gaagatgcag caggaattag gggataaaaa acaagagcac ctggtggcac tctatctcaa
     8881 tactcaaaat caaatcatcc atcagcagac catttttatc ggttctgtaa ctcgtagtat
     8941 cgctgaaccg cgagagattc ttcactatgc aatcaagcat atggcgactt ctcttatctt
     9001 ggtccacaat catccttcag gagcggtagc gcctagccaa aatgatgatc atgtcactaa
     9061 acttgttaaa gaagcctgcg aactgatggg gattgttctc ttggaccatt tgattgtctc
     9121 tcattctaat tactttagtt atcgtgaaaa gacagattta atctaaagtt cattaacgac
     9181 atagtcaaag agttttttat ctttgggacg attttcaaaa agaagttctg gatgccattg
     9241 gacaccgaga aaggcgacat catccgtact catgacagcc tcaatgatac catctttagg
     9301 atcatgagcc acaactttta aatttggtgc taagtccttg atgctctggt ggtggaagga
     9361 gttgatatga gagatttctc catagatttc ttggagaacg gtatctggtt ctgttaccaa
     9421 gcgttgagtt gtgtactcaa cagaagaatc ctgccaatgg tcttcgatat cttggtacaa
     9481 agttccaccc atggcaacgt taaagagttg ggtaccacgg cagacagaga aaatgggctt
     9541 tttctgttta atagcttcct tgatgagggc cagttcgaag atatctcttt gaaggtgata
     9601 gtcatcgcta tcaatggttt tgggttcgcc atagaatttt ggatcgacat tttgcccacc
     9661 tgtcaagatg agcttgtcaa tcaaactgat atagtggcag gccatttctt gatcaccaat
     9721 cggtaggatg atgggaatcc ctccagcatc tttaacgcct tcaacaaagc cttttgctgc
     9781 gtagctcatc atgatgtcat catctggatg agctttttcg tttcctgtaa tcccaataac
     9841 tggttttttc ataaaatgat tttcgctttc taatcctctt ttcgcatgaa gtagaggagg
     9901 gtttggagtt cacttgtcaa atcgacatac tgaacgacca cgtcttttgg taaatgcaga
     9961 tggactggtg aaaaactgag aattcctttc acaccagcat caaccaagag attagcaacc
    10021 tcttgtgact tgacgctggg aacagttagg atagcagtct tcacatcagc atccttgatt
    10081 ttatccttga tctgagaaat cccgtaaatg ggaatcccgt caggagtttg ggtaccgact
    10141 tcaggatggt cgtctaggtc aaaggccatg ataatcttca tcttgttacg ttcgtggaag
    10201 cggtagtgga gaagggcatg gcccatattt ccaataccaa ccagcatgac attggtaata
    10261 gagttgtcat tgagcaaatc ggcaaaaaat gtcattagtt ttttgacatc atagccaaaa
    10321 ccacgacgac caagttcacc aaaataggaa aaatcacgac gtacggtcgc tgaatcaata
    10381 ccgatagcct ctgcaatttg cttagagttg gcacgttcaa tcttttctgc atgaaatctc
    10441 ttaaaaattc gatagtagag agagagtctt tttgctgtag cttttggaat agcaaactgt
    10501 ttatctttca caaaatcaca ac
//
LOCUS       DAVLTM010000066        10430 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLTM010000066 DAVLTM010000000
VERSION     DAVLTM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.067"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(108..857)
                     /locus_tag="VYN16_001419"
     CDS             complement(108..857)
                     /locus_tag="VYN16_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1403937.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            990..3326
                     /locus_tag="VYN16_001420"
     CDS             990..3326
                     /locus_tag="VYN16_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1403938.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3855..5318
                     /locus_tag="VYN16_001421"
     CDS             3855..5318
                     /locus_tag="VYN16_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1403939.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            5311..5739
                     /locus_tag="VYN16_001422"
     CDS             5311..5739
                     /locus_tag="VYN16_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403940.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            5717..6166
                     /locus_tag="VYN16_001423"
     CDS             5717..6166
                     /locus_tag="VYN16_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1403941.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            6194..6505
                     /locus_tag="VYN16_001424"
     CDS             6194..6505
                     /locus_tag="VYN16_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV1403942.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            6531..7616
                     /locus_tag="VYN16_001425"
     CDS             6531..7616
                     /locus_tag="VYN16_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEV1403943.1"
                     /translation="MLKHLNLKGHLLTAISYVIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            7627..8322
                     /locus_tag="VYN16_001426"
     CDS             7627..8322
                     /locus_tag="VYN16_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1403944.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLKSTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            8359..10329
                     /gene="tkt"
                     /locus_tag="VYN16_001427"
     CDS             8359..10329
                     /gene="tkt"
                     /locus_tag="VYN16_001427"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1403945.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMPLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
BASE COUNT         2962 a         1868 c         2375 g         3225 t
ORIGIN      
        1 agtcaaatcg atttctaaaa atgttttaag aagtagaggt gtactattct agtttcaatt
       61 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
      121 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      181 atagcgagga taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      241 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      301 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      361 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      421 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      481 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      541 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      601 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      661 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      721 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      781 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      841 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      901 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      961 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
     1021 aaagagaagt caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1081 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1141 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1201 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1261 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1321 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1381 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1441 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1501 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1561 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1621 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1681 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1741 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1801 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1861 gcgtggatat gctctgtctg gacaagacgg gcaccatcac ccaaggaaag atgcaggtgg
     1921 aggctgttct tccactgacg gaaacttacg gtgaagaggc tattgccagc atcttgacta
     1981 gctacatggc ccatagtgag gataagaatc caactgccca agccattcgc cagcgttttg
     2041 tgggagatgt tgcttatcct atgatttcca atcttccctt ctcgagcgac cgcaagtggg
     2101 gggctatgga gttagaaggc ttggggacag ttttcttagg ggcacctgag atgttgctgg
     2161 attctgaagt cccagaagcc agggaggcct tggagagagg atcacgtgtc ttggtcttag
     2221 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2281 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2341 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2401 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2461 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2521 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2581 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2641 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2701 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2761 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2821 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2881 tgattgacca gtttgtggaa ggtttcccac catttgttct gacttttgag cgaaatatca
     2941 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     3001 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3061 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3121 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3181 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3241 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3301 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagtttttta
     3361 tacgatggat ctatcgtaga tctgatgtgc aaggcctacg tgtctattat ctatcggtga
     3421 acccaagagt gattctcaag cctgcttgga ttgaggtaat agattcaaat atctgtagtt
     3481 agactatttg aagtttgatg taagaaagag aaagcgacag attgaagtaa ttttaactct
     3541 cttctattgc tagaacaaat ggtcggatag gttggtagtt tgaaaatgaa gatgcaatct
     3601 attgttaaat ggaacatagt gttatttatt agaaaatcgt ttggtttatt tcttatcaaa
     3661 tacgaaaagc aacttaaata tttcaactaa aatagatgtt atgaagaaaa ggtaaaatga
     3721 ttttggtata gtgaggttct gttctatttg atatcatatt tttgataaaa acaaaaatgt
     3781 ccattgcaaa ggacaaaatg cgaagtatat tattttttga aagcgatata atggaatcat
     3841 aaaggaggtg tatcgtgtct agaaaacaag aacaaatgga aacgttgttg ctccttttgc
     3901 gagatagtaa ggattatata tctgctaaag tattgggaga aaaattaaat tgctctgata
     3961 aaacggttta tcgccttgtc aaggtaatca acaaagattg tccggtagaa gcattcattt
     4021 tatctgaaaa aggcagaggt ttcaaattaa atccaagaag ttccctcgtg gacgttgatg
     4081 ggaattttac agaggctttt gatcctgaag taaggcgtga aaaattacta gaacgtctct
     4141 tgttgactgc tcctaagcca cattctattt atgatttagg agaggaattc tacgtaagcg
     4201 agtcagtagt actaaaagat cgtcagatat tacaagagag tctagcaatt tatgggttag
     4261 atttaaaaat gagacaacga aagcttttta ttgatgggga tgaggctcaa attcgttcag
     4321 ccattctaaa tctactgcca atgtttaatc agttggattt agagcaaatt acacagaata
     4381 aggttcagcc tcttgacgga gaacttgctc acttttgttt gggattactg attacacttg
     4441 agagagaatt gggggtaaac attccctatc catataatat aaatattttc tctcacctgt
     4501 atatttttat cagtaggaat cgtcgtagta ctagtattca tgttgtagca ccttcaaaac
     4561 ctactattgt tgatgagaaa atttacagtg tctgtcaaaa aattattcaa gaaattgaac
     4621 aatattttaa gatgaaggtt gatgcagttg agattgacta tctttatcaa tacgttgtat
     4681 cttcgagatt gcaaaaacca ttttcttccg ggaagcttcc tttttctcag cgagttttag
     4741 atgtcactca ttactatttt agccgtatgt gtatggacaa tagagagatt gaaacgacag
     4801 atcctgactt tgttgacttg gcgagtcata tcagtccctt actgaggaga ttagataata
     4861 gagtacagat taagaatagt cttttatcac aaattctttt aacctatcct aatctagtta
     4921 aagagttaac aactatttct aaagaagtga gtctagtatt tggttttgcg tccttgagtc
     4981 tggacgagat tggttttcta gtcttatatt ttgcacggtt tcaagaaaag cgagcacgtc
     5041 ctctaaaaac agtagtgatg tgtacatcag gtgtcggaac ttcagagctt ttacgagcac
     5101 gattagaaaa gcaatttact gaattggata ttattgatgt agttgcttat catcaattag
     5161 atgagctgat aaatctatat ccagatttag atttcattgt gacgacggta gctttgcagg
     5221 aaccagcaag tgtcccgttt gtcctagtta gtgtttttct aaccgagggt gataaacaac
     5281 gtcttcaagc aaaaattcag gagataaact atgaataatc tttcgcttgt ccttatggat
     5341 atatctgttc aaaatcgtca agaagcctac aaagaattag caaatcaaat cagccttctt
     5401 gtttctgaag atacagaaaa aatagaagag cttctatatt accgtgagag acagggaagt
     5461 atagaggttg ctaaaggtgt tcttctacca cattgtgaag gaaactttca acatcatgtc
     5521 ttagtgatta ctagattaaa atcacctatc agagaatggt cgaaggatat ccagtgtgtt
     5581 gaccttatta tcggtttggc cattgcagta tcacaggaca agtcatgtat taaaacattg
     5641 atgagaagac tagcagatga atcattcata aatcaattaa aacagttaac aaaagaagaa
     5701 ttacgggaga taatatatgg aaatcaaaga tattcttaat gtgagtctga tccagacgga
     5761 tttacagatg cagagcaaag aagaggtttt tgaggcatta gctcaactat tggttgagac
     5821 gggttatgtg tctgatagag accaatttat cgaaggtctg tatcagagag aggcagaagg
     5881 acagaccggt attgggaatt atattgctat tccccatagc aagagttctg ctgtggagaa
     5941 ggcgggggta gtcatagcta taaatcacaa tgagattcct tgggagacca ttgatgggaa
     6001 aggggtcaaa gtaattgtac tctttgcagt tggtgatgat acagaagctg ctagggagca
     6061 tttgaagacc ttatcactct ttgctcgaaa acttggtaat gacgaagttg ttgccaaatt
     6121 agttcgggct cagacatctg atgatgtgat tgcagctttt tgttaataag aaaaaatttt
     6181 ggagggtatc cgtatgaaaa ttgttggtgt tgcagcttgt actgtgggaa ttgcccacac
     6241 ttatattgca caggaaaaat tagagaatgc cgcaaaggta gctggacatg tgattcatgt
     6301 tgagactcag gggacaatag gggtagaaaa tgaattgagt caagagcaga ttgatgcagc
     6361 ggatgtagtt attttagcag ttgatgttaa gatttctggt atggaacgct ttgagggtaa
     6421 aaagattatc aaggttccaa cagaagtggc agtcaaatct cccaataaac tgattgctaa
     6481 agctgttgag attgttacga aataactgaa aatatttaag gagaaaatat atgttgaaac
     6541 acttaaactt aaaaggtcac ttattgacag ccatttccta tgtgattcca attgtttgtg
     6601 gtgcaggatt cttagttgcc attggtttag caatgggggg tggtgttcct gacgctcttg
     6661 tagcaggaaa attcactatc tgggatgctt tagcaactat gggtggtaaa gcccttggtc
     6721 tcttgccagt tgttattgct acaggtttgt cttactcgat tgctggtaag ccagggattg
     6781 caccaggttt tgttgttggt ctaattgcca attctgttgg ttcagggttt atcggtggta
     6841 tcttgggagg ttatatagct ggtttcttgg ttcaagcgat tattaaaaag gtcaaagtac
     6901 caaactggat taaaggttta atgccaacct tgattattcc ttttgtagcc tctttggtaa
     6961 gtagtttgat tatgatttat attattggag cgcctatcgc agcctttacc aactggttga
     7021 cgagcttatt acaaagcttg ggaagtgctt caaatggttt gatgggggca gttattggag
     7081 ttctcagtgc tgttgacttt ggtggcccac ttaataaaac agtctatgcg tttgtgttga
     7141 ctttacaggc tgaaggtgtg aaagaaccat tgactgcttt gcaattggtg aatactgcta
     7201 caccagttgg atttggattg gcctatttta tcgcgaaatt actcaaaaaa aatatctata
     7261 ctcaagagga aatcgaaaca ttgaaatcgg ctgttcctat gggaattgtc aatattgttg
     7321 aaggtgtaat tccgattgtt atgaataact tggttccagg tctcattgca acaggtatcg
     7381 gtggtgctgt tggtggtgct gtttctttga caatgggtgc tgattctgcc gtgccatttg
     7441 gtggagtgct tatgttacca accatgactc gtccagtagc tggtatttgt gctttgttag
     7501 ccaacattgt agtcacagga cttgtctacg cgattttgaa aaaaccaata aaacatacag
     7561 aaccagttat gactgttgaa gaagagattg atttgtcaga tattgaaatt ttgtaagagg
     7621 gtaacgatgt caagaattga attttcacca tctttgatga ccatggattt ggacaaattc
     7681 aaagagcaga ttactttttt gaatgataaa gtagcatctt atcatatcga tattatggat
     7741 ggccattttg ttcccaatat taccttgtct ccttggttca ttcaagaagt tcaaaaaatt
     7801 agtgacacac ctttatcagt tcatctgatg gtcacagacc caaccttttg ggtagatcaa
     7861 gttctcgatt tacaatgtga gtatatttgt attcatgctg aagttctgaa tggtcttgct
     7921 tttcgtttga ttgataaaat tcatgatgca ggtctaaagg ctggtgttgt ccttaatcct
     7981 gaaacacctg tttctacaat ctttccctac attgatttac ttgacaaagt aactattatg
     8041 actgtagatc caggttttgc aggacaacgt tttttgaagt ctaccttgta taaaatccaa
     8101 gaactccgtc agcttagagt tcagaatggt tatcactaca tcattgagat ggatggttct
     8161 tcgagtcgta agactttcaa acaaattgat gtggcaggac cagatattta tgttataggt
     8221 cgcagtggat tatttggttt ggatgacgat attgccaaag cctgggatat ctgttctaga
     8281 gattacgaag aaatgaccgg aaaaacaatg ccaatcaaat aatggtttga gaagaaattt
     8341 attagttagg aggaatatat gtcactacaa tcagttaacg ccattcgttt tcttggcgta
     8401 gatgctatta acaaatctaa ttctggtcac ccgggaatcg tcatgggtgc tgcgccaatg
     8461 gcttatagct tatttacaaa acaccttaga attacacctg agcagccaaa ctggattaac
     8521 cgagatcgct ttatcttgtc tgcgggtcat ggatcaatgc cactgtatgc tctcttgcat
     8581 ttaacagggt ataaggatgt atccatggac gagattaaaa atttccggca atggggatct
     8641 aagacacctg gtcatcctga agtgacgcat acgtctggtg tggatgcgac atctggtccg
     8701 cttggtcagg ggatttctac tgccgttggt ttcgcccaag cagagcgttt tttagctgct
     8761 aagtacaaca aagatggttt tcctattttt gaccattata cttatgttat cgctggagac
     8821 ggtgacttca tggaaggagt gtctgcggag gcggcttctt atgcaggtca tcaagcttta
     8881 gataagctta tcgtcctcta cgactccaac gatatctgct tggatggtga gaccaaagat
     8941 actttctctg aaaatgttcg cgcccgttac gatgcttatg gttggcatac agttctggta
     9001 gaagatggaa cagatttagc agcaatttct acagcaattg agacggccaa gttttctggt
     9061 aagccgagtt tgattgaagt gaaaacggta attggttacg gctcacccaa taaaagtggt
     9121 acaaatgctg ttcatggtgc accactagga gcagaagaaa caggagcaac tcgtaagttt
     9181 ttgggatggg attacgatcc atttgaagta ccagaggaag tatattctga tttcaagaca
     9241 aatgtagcgg atcgtggtca ggaggcatac gatgcttggg ctagtttggt gtctgattac
     9301 aaggttgctt atcccgaagt tgctagtgag attgacgcta ttgtagctgg aaaatcccct
     9361 gtaaccatta ctgaaaaaga cttccctgtc tatgagaatg gcttctctca agcaactcgt
     9421 aattcgtccc aagatgctat taatacagca gcagcagttt taccaacctt cttaggtgga
     9481 tcggcagact tagctcactc caacatgacc tacatcaagg cagatggctt acaagataaa
     9541 tataatccat taaaccgcaa tattcagttt ggggtacgtg aatttgccat gggaacaatc
     9601 ctcaatggaa tggctcttca tggtggttta cgagtttatg gcggaacctt ctttgttttc
     9661 tctgactacg tcaaagctgc tattcggcta tcagccattc aggagttgcc tgtaacttat
     9721 gtctttaccc atgattcaat tgccgttggt gaagatggtc caactcatga accagttgaa
     9781 catttggcag gtttacgctc aatgccaaac ttgactgtta tccgtccagc ggatgcccgt
     9841 gaaactcaag cggcttggca tcatgccttg accagtacca ccactccaac tgtcattgtc
     9901 ttaacccgtc aaaacttggt agttgaagaa gggacagact ttggtaaggt cgctaaagga
     9961 gcctacgtcg tgtatgatac cccgggattt gatactatta tcattgctac aggatctgag
    10021 gtcaatctag ctatcaaagc tgctaaggaa ttggttttac aaggtggtaa agtacgtgtg
    10081 gtatctatgc cctcaaccga actatttgat gctcaagatg ctacctacaa ggaagacatt
    10141 ttaccatcta agactcgtcg tcgtgtggcc attgaaatgg cagcgaccca aagttggtac
    10201 aagtatgttg gtttggatgg cgcggtcatc ggtattgaca tcttcggtgc gtctgcccca
    10261 gctcagactg tgattgataa ttatggattt acggtagaga atatcgttgc tcaagttaag
    10321 tccctataga aaccaattac aatgaagata cagctgttgt cagactagca gatgtagtga
    10381 tagacactaa tcagatgatt ggttatttaa aaactataat gagaatgtaa
//
LOCUS       DAVLTM010000067        10396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLTM010000067 DAVLTM010000000
VERSION     DAVLTM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.068"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..505
                     /locus_tag="VYN16_001428"
                     /pseudo
     CDS             1..505
                     /locus_tag="VYN16_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            405..851
                     /locus_tag="VYN16_001429"
     CDS             405..851
                     /locus_tag="VYN16_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1403946.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVDHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(989..2293)
                     /gene="eno"
                     /locus_tag="VYN16_001430"
     CDS             complement(989..2293)
                     /gene="eno"
                     /locus_tag="VYN16_001430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1403947.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2457..2903
                     /locus_tag="VYN16_001431"
     CDS             2457..2903
                     /locus_tag="VYN16_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1403948.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2900..4015)
                     /locus_tag="VYN16_001432"
     CDS             complement(2900..4015)
                     /locus_tag="VYN16_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1403949.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENRYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4024..4669)
                     /gene="serB"
                     /locus_tag="VYN16_001433"
                     /pseudo
     CDS             complement(4024..4669)
                     /gene="serB"
                     /locus_tag="VYN16_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4728..6161)
                     /gene="glgA"
                     /locus_tag="VYN16_001434"
     CDS             complement(4728..6161)
                     /gene="glgA"
                     /locus_tag="VYN16_001434"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1403950.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6158..7297)
                     /gene="glgD"
                     /locus_tag="VYN16_001435"
     CDS             complement(6158..7297)
                     /gene="glgD"
                     /locus_tag="VYN16_001435"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1403951.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7287..8429)
                     /locus_tag="VYN16_001436"
     CDS             complement(7287..8429)
                     /locus_tag="VYN16_001436"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1403952.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVTTDED"
     gene            complement(8419..10347)
                     /gene="glgB"
                     /locus_tag="VYN16_001437"
     CDS             complement(8419..10347)
                     /gene="glgB"
                     /locus_tag="VYN16_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1403953.1"
                     /translation="MDNREALKTFMMGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNIEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3151 a         2300 c         1839 g         3106 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat ccactatgcg
       61 ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga
      121 aaaagtagcc ttatttctta agaattttaa tagtttaaag cacctagcac aggtctaaca
      181 aataaatggt gagttaatcg ctccaatgat ttacgaagag acgatgacga gcgacttttt
      241 tgaagcttgg tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat
      301 ggataatgca agattccata gaatggggaa gctagaactt ttatgcgaag agtttgggca
      361 taaactttta cctcttcctc cctactcgcc tgagtacaat cttattgaga aaacatgggc
      421 tcatatcaaa aagcacctca aaagggtatt accaagttgc aatacctttt acgaggctct
      481 tttgtcttgt tcttgtttca attgactata taaattgtct aagcgaaaca accgataaga
      541 attggcacaa aagcgaccgt atttttgtta ccaatacagg aaaaccagtt catagttcta
      601 tcttgagcaa gtctctccag cgagcaaacg aacgccttaa aaaaccaatt cccaaacatc
      661 tgtcccctca catcttcaga cacaccacta ttagcatctt atcagaaaat aaaattcctt
      721 taaaaacaat cacggacagg gttgatcatt ccgactctga agtcactact tccatctaca
      781 cccacgtcac aaagaacatg aaagatgaag caatcaatgt actggataaa gttatgaaaa
      841 agatttttta aaaagttttg tccctttttt gccctctaaa tacaaaaata gcccttcgga
      901 taaaatccga ggggctagaa acgttgttaa atcaacggcc gaacttttga atttcatggt
      961 tcgggataaa atagttcact gaactatttt attttttaag gttgtagaat gatttcaatc
     1021 cacgatattc agctacttca ccaagttggt cttcgatacg aagcaattgg ttgtatttag
     1081 cgatacggtc tgtacgtgaa agtgaaccag tcttgatttg tcctgcgtta gttgcaactg
     1141 cgatatcagc gattgttgaa tcttcagttt cacctgaacg gtgtgataca acagcagtgt
     1201 aaccagcttc tttagccatt tcgatagctt caaaagtttc agtaagagta ccgatttggt
     1261 taactttgat aaggattgag ttagcagcac cttcttggat accacgtgca aggtagtcag
     1321 tgtttgttac gaagaagtcg tcaccaacaa gttgtacttt cttaccaaga cgttcagtaa
     1381 gagctttcca accatcccag tcgttttcat ccataccatc ttcaatagtg atgattgggt
     1441 atttgttaac caattcttca aggtagtcga tttgttctgc agatgtacga acagcagcac
     1501 cttcaccttc aaatttagtg tagtcgtaaa ctttacgttc tttatcgtag aattctgatg
     1561 aagcacagtc aaatccgata aatacgtctt tacctggtac atatccagca gcttcaatcg
     1621 cagcaaggat agtttcaaca ccatcttcag ttccttcgaa acgaggagcg aatccacctt
     1681 cgtcacctac ggcagtttcc aaaccacgtg atttaaggat tttcttaaga gcgtggaaga
     1741 tttcagcacc gtaacgaagg gcttctttaa atgttggcgc accaactggc aagatcatga
     1801 actcttggaa agcaattgga gcgtcagagt gagaaccacc gttgatgatg ttcatcattg
     1861 gagttggaag aactttagtg ttgaatccac caagatagct gtaaagtggg atttcaaggt
     1921 agtcagcagc agcacgagct acagcgatag acacaccgag gattgcattc gcacccaatt
     1981 tacctttgtt aggagtaccg tcaagtgcga tcatagcacg gtcaatagct tgttgatcac
     2041 gtacatcgta gccaatgata gcttcagcaa tgatgttgtt tacgttgtca acagcttttt
     2101 gtgtaccaag accaccgtaa cgagatttgt caccgtcgcg aagttcaact gcttcgtgtt
     2161 caccagtaga agctcctgat ggaaccatac cacgtccgaa agcacctgat tcagtgtaaa
     2221 cttctacttc aagtgttggg ttaccgcgtg agtctaggac ttcgcgagcg taaacatcag
     2281 taataattga cattttttac tctccttatg agttaaattt tttacacctc tataatacct
     2341 taaaacccct cctttttcaa gaaaaaacgt tatctttgtg caacttttcc ttaactttat
     2401 aaagtaatcg ctttcttttg tctgttttat tctaactttt atgatatact gttttcatga
     2461 cagatttatc aaaacaatta cttgaaaaag ctcatggtgg gttaaaaata aatccggatg
     2521 agcaaagacg ctatcttggt acttttgagg aaagagttct tggatatgta gatattgaca
     2581 cagcaaatag ccctcagtta gaaaaaggct ttttatttat tttagaaaac cttcaggaaa
     2641 aagcagagcc actatttgtg aagatttcac caactatcga atttgataag caagttttct
     2701 acttaaaaga agcaaaagaa actgatagtc aagccaccat agtatctgaa gagcatatta
     2761 cttctccttt tggcctggtt attcatagca atgcaccagt tcaagtagaa gaaaaagacc
     2821 ttcgacttgc ttttccaaaa ctttgggaag ttaaaaagga agaaccagcc aaaacatcct
     2881 tatggaagaa atggtttagc taaatcttgc acatatttaa taagtgccca atattggcag
     2941 ccgtgcgctc cagatagaga ctggcatttt tcaaactatc ttctaaaggt tcacttttct
     3001 ccaaaataga aaagacagct tggatatttt caaatggtag gggaggtaaa tcttcagcaa
     3061 gactaccgca aatagcaata acaggaactc caacaggggt tctttttgca acacctatag
     3121 gcgctttccc agcaaagctt tgactatcaa gtcttccttc tccaacaaca accaagtcag
     3181 catctgaaac tttcttatca aagttgatta agtccaagca ggtatcaatt ccagacacga
     3241 tacttgcctg agcaaaggca cacaaaccac cagcaaggcc tccacctgct cctgctcctt
     3301 taatttctaa tgttgcaggt gagaattttt cataaaaatc ttggatcgcc tgatctacta
     3361 ctgcaaacat agtcggatgt agaccttttt gattgccaaa agtgtaagtc gcaccttgat
     3421 gaccacataa gggactcacg acatctgcta aaatatgaat ttgaacacct tcaggaattt
     3481 tatagcgatt ttctgttgaa acagaagcta agtttaataa ggattgaccg gaagcaggca
     3541 agacatttcc atccctatca taaaattgat aacctaaacc agcagcaatc cccagtcctc
     3601 catcattact ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga
     3661 gatggagaat caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta
     3721 gcggaatttt tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt
     3781 gaaaatagcg catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt
     3841 ttaggtcaag agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc
     3901 agaggagaca ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta
     3961 cctgttgagc tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt
     4021 ccctcattct aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg
     4081 atgtggtatt tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga
     4141 cttcaacatt aatagattat tgaccccatc accgattgcc accgttcttt ctttagaaag
     4201 ttttagtttc tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg
     4261 tccaactaat tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata
     4321 ggcaatacca agggattttg ctaatctctc caactattgg tgtaaatcca ccaggcacca
     4381 gaccaactag gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata
     4441 gatgaattga gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca
     4501 ctctttttct taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct
     4561 gcgaaatttc cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga
     4621 ttaaggttcc atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc
     4681 tctaaacagc ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa
     4741 ctatggtaca agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata
     4801 gcttgttttt gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt
     4861 tggaaagtcc aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt
     4921 ccttcgattg gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat
     4981 ggcaaggttc cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt
     5041 ggcatgagga agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg
     5101 atatttgttg atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag
     5161 gctggatcgc cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga
     5221 ctttcgacca ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt
     5281 ggaacgtctg ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct
     5341 ttcccagaca aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta
     5401 taaagatcag catcaatccc attcacgata ccagatactt taccagactc cattttaaga
     5461 atctgatcca aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa
     5521 acggttgaaa cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc
     5581 catcgaaggg tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt
     5641 ccttctgaaa attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc
     5701 tcataggctt gaatccaacg gtatttttcc ttcaacaaga aaggaatcat agctgtatgg
     5761 tagtcatgaa catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca
     5821 gccagttgga aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg
     5881 aagaaataat attgattgtc aataaagtag aaggttacac catttaatac tgttttctta
     5941 attccacaat actgtctgcg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc
     6001 tgatttccaa atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc
     6061 ccagctttta ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt
     6121 gaaaagggtg caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta
     6181 ctttagcacc tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccag
     6241 gctcaacttc aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa
     6301 caacacgagg gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac
     6361 gtgatagaac agaattagct acttgacctt caataatact accagaggca aactgagaag
     6421 tgcttacctt agatgtatta gcatagtaag ttggctcttc gtttttgacc tttgtataaa
     6481 tcttttggtt tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatattcg
     6541 cttgataata agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcga
     6601 aagctccctc ttttacagcc aaatcccgta aaacatagcg caatttcttt ggatgttctt
     6661 ttttagcttc ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg
     6721 tagacatatt gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat
     6781 ggtctgtttc atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt
     6841 ttttataaac tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca
     6901 aatcaatgtt aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt
     6961 tcaaataagt aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg
     7021 tattgtaaat tcctagatag taatgactaa gaagggttga taaaccccac tcgcgtcctg
     7081 aacgaatatg gtcaaatact gagctgatat tatcctgctg gaaaatacca aagacactac
     7141 gaacacctgc attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa
     7201 atggcaaact tgctactgga cggtggtccg tcaatgtcga catattgtga aaaccaactg
     7261 tatttcctaa aatggcagaa tatttatcaa tcttcatctg ttgttacccc cactacttca
     7321 ttatatccta caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc
     7381 gcaccttcac caataatggc acgtttaatc ttagctcctt gaccaatgat agctccactc
     7441 atgataactg aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata
     7501 gaatgtttaa cagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttctacatga
     7561 gcatttgccc cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga
     7621 cggttacgac tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt
     7681 gactcaatag taccaacatc tttccaataa ccactaaatt cgtaagcata aacactttca
     7741 cctgactcaa ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc
     7801 ttttcagcag cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata
     7861 gaagcttttg tagatttagg ttgagctggc ttttcttcaa attcaacaat acgattgtta
     7921 gcatctgtgt tcatgatacc aaaacggctt gcttctttaa gagggacgtc taaaactgct
     7981 actgtcaagc tggcattatt atccttatga gactggagca tatcatcata gtccattttg
     8041 tagatgtgat ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg
     8101 atattttggt aaatagcgtg actagtcccc tcaaaccaac gatttccttc acttgcagaa
     8161 taaggttgaa gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc
     8221 ccaatatggt tgttgagagc aagtggttga tactgtgtaa cgaccccaac attatgaatc
     8281 cctgagttgg cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc
     8341 acagctggtt ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca
     8401 agaatcaaag ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac
     8461 ggttttagta gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag
     8521 agtaaaggtt aaggtctgct cataatcttt ccatagtcct tcttgcgttt gaacagtttg
     8581 attatgttct ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta
     8641 aattcctgca acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa
     8701 gatacagact aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc
     8761 tcgattatcc gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca
     8821 gcgatgatct ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat
     8881 tgggtcttct atgttagacc attccaactg ttcttcagat ttccattcta ggaattgacc
     8941 gtattcgcta cccatgaaga gcaatttctt accagggtga caaatttggt acgtatagag
     9001 attgcgcaag cctgcaaatt gattgtaacg atctccccac atcttatgca tcatactctt
     9061 cttgccatga accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata
     9121 cataaagctg aaagtcacca ggttaaagtc atatttacga taaatcggat cttcttcgta
     9181 gaaacggagg atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc
     9241 aatctctttc attcccgtaa tcttggttgc agacgaactt tcttctgcaa tcatcatcac
     9301 atctggatat tctaacttaa taacttcatt caagcgttga aggaaataat aaccttcata
     9361 gttgagattt ccgccatctt tattaggtgt ccatggagca tcatcatagt ccaaatagag
     9421 catgttgcta acagcatcca cacgaatacc atccaaatga tagatatcaa tccaatgctt
     9481 aatgcaagaa attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc
     9541 ccaaccatgg ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata
     9601 ataggctaag gcatcatcat tgatggtaaa gtgacctggt acccagtcca caataacccc
     9661 aatattatgg gtatgacact cctcgacaaa atcttgaaac tcctctggtc taccataggc
     9721 atgctctaaa gcgaagtaac ccataagctg gtacccccaa ctcaaaccta gtgggtggga
     9781 catcaagggc ataaactcaa tatgagtata gttcatctca actaggtaag gaatgagttc
     9841 atctttcaat tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc
     9901 gtgaacttca taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag
     9961 ccaaagtcca tccttccatt tcttctcagg aagctctgtt aagattgccc ctgttcctgg
    10021 acgagcctca tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg
    10081 tgtgacatga tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac
    10141 cccaaaatca tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac
    10201 caagtgaaca gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt
    10261 tagttcttcc ctatgtgctc ctagataatg ttggagataa aaattttcac ccatcataaa
    10321 ggtttttaat gcttctctat tatccatata ctccctttct cctgtaagcg ttttctatgt
    10381 ttttattata ctacct
//
LOCUS       DAVLTM010000068        10131 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLTM010000068 DAVLTM010000000
VERSION     DAVLTM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.069"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(314..1624)
                     /locus_tag="VYN16_001438"
     CDS             complement(314..1624)
                     /locus_tag="VYN16_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1403954.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1861..2733
                     /locus_tag="VYN16_001439"
     CDS             1861..2733
                     /locus_tag="VYN16_001439"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1403955.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            2864..3710
                     /locus_tag="VYN16_001440"
                     /pseudo
     CDS             2864..3710
                     /locus_tag="VYN16_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3796..4746
                     /locus_tag="VYN16_001441"
     CDS             3796..4746
                     /locus_tag="VYN16_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1403956.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVFSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            4923..5930
                     /gene="gap"
                     /locus_tag="VYN16_001442"
     CDS             4923..5930
                     /gene="gap"
                     /locus_tag="VYN16_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1403957.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(5997..6833)
                     /locus_tag="VYN16_001443"
     CDS             complement(5997..6833)
                     /locus_tag="VYN16_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1403958.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            6959..9154
                     /locus_tag="VYN16_001444"
     CDS             6959..9154
                     /locus_tag="VYN16_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1403959.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQVVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9207..9359
                     /gene="rpmG"
                     /locus_tag="VYN16_001445"
     CDS             9207..9359
                     /gene="rpmG"
                     /locus_tag="VYN16_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1403960.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9369..9545
                     /gene="secE"
                     /locus_tag="VYN16_001446"
     CDS             9369..9545
                     /gene="secE"
                     /locus_tag="VYN16_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1403961.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9600..>10131
                     /gene="nusG"
                     /locus_tag="VYN16_001447"
     CDS             9600..>10131
                     /gene="nusG"
                     /locus_tag="VYN16_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1403962.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         3156 a         1774 c         2216 g         2985 t
ORIGIN      
        1 gatttgacta tcctgatcta tttgtcctgt tcttatttca tttcactata tctcaaattg
       61 agtatgacga agtgcgctcc catgtcctgg gaacgcactt tcttcatatt tttcatattc
      121 ttgaatccat cgataaagac tattgggatg aatttttaaa gttgaactaa tcatttttac
      181 aggatgagat ttacagcaga gagtttgaag gctttatcaa aggtttttct tggcataatg
      241 acttttcctc gtttccactt aattttgtgt ctactttatt ataccaagtc cacccttaag
      301 ttagataata aatctaactt aaggaagcta gaaggatgag aatccaggtg gtcaagagtc
      361 ccaaacttaa gctgatgggg acacccagaa taatttgctt tttgaaggca aggccacgtt
      421 cctctatatt gggaagtgag agttgaatga gagaaccagc tgatgaaaag ggtgagatat
      481 tagtagatag agcgccaata acggtggctg ttgtgagtaa gtgaatatca atctgaggat
      541 tttgagcact gatgatagca atgatgggaa agagggctgg agctacaacg gatagggtgg
      601 aactaaagag tgacatcact ccggctatca cacaaaagaa cagaggtaac cagaaatgag
      661 gaatggttgt tgtcatgagg tgccctatca gtgtgactaa acctgacttg accgctagag
      721 acattagtaa gctcatgccg cagagcatga taattgtagc ccagggaacc ttagctaaaa
      781 tggcttcttg cttccctaat ttgagcctta aggcgaggca gaccatgagt attgagacaa
      841 agccaatatc aaatgttttt tgataagtag ctatccaggc gatgtttggg aaaatgagat
      901 gcaacaaggg aaaaagccaa accaaaacca tgctgctgat catgagcaag gtggtttgtc
      961 tttgaacctt gctgaggagt ggtggttggt caatagtcaa ggatgagttt gttcttccct
     1021 tactatagtg actgtaacag gataataaaa gcaagacgat gagtgggtag ataatgctga
     1081 cgataaagat atgattgcca agtgaaaaag cttgctcctc ccatcccatt tgcttaaaca
     1141 ggccttgaaa gacaatgcct gagctactgg ttatcaaatt agcccctcct gaagctcccc
     1201 aattgacggc ttgagctcca atcaaagggt gtttgtccgc tttttgacag agggtaatcg
     1261 ctagaggaca gcaaacggcc atagtagtga aaaatccagc acctaaagca gacaaaaggg
     1321 ttgccatcag gtataaaatc atgtagaggg cgttagggtg ggtgcgtgtg cggtagagaa
     1381 tgtgttgagc caaaacatca agagtaccgt tagttgttgc aacgttataa aagagagaga
     1441 cgctaaaaat ggtaaaaaag agtgaggttg gccaaaaatg aagaagttct ttggggctta
     1501 atcccatgag agtggttgcg atgaggtaag aaaaagcaat agccagcagg ccaatattga
     1561 ttttggtgcg gtaaccaatt ccaatggcta gagcaatggc gctaatcatt attaaatgaa
     1621 tcattgaatt gtcctttcag ttagaatatg gaaataggtt ggcaattttg gcgctcaaaa
     1681 gaccacctgt ttcctattgg tacttgacta ttatacacaa aaaaaataca attgtcttga
     1741 caattgtatt gacacttgtt agaattaaaa atgaggtatt caggcttgtg atttcctatg
     1801 gaagttaata gtaattgcct ctagtgctta caagtgatat taaaaataga ggacttagtg
     1861 atgtcaatca tttcaactga tttaacccct tttcaaatag atgatacatt gaaagcagcc
     1921 ttgcgagaag atgttcattc cgaagattac agtaccaatg ccatttttga tcatcatggc
     1981 caagccaagg tgtcgctttt tgccaaggaa gctggtgttt tagcgggact aaccgttttt
     2041 caaagggttt ttaccctatt tgatgccgag gtgaccttcc agaatcctca tcaatttaag
     2101 gatggggatc gtttgactag tggcgatttg gttttagaaa tcataggctc ggtgagaagt
     2161 ctcttaacat gtgaacgcgt tgccttgaat tttttacaac atttatcagg gattgcttcg
     2221 atgacagctg cttatgtaga agccttaggc gatgattgca ttaaggtatt tgatactcga
     2281 aaaactactc ctaatttacg tctttttgag aaatatgccg tgagagttgg cggtggctat
     2341 aatcatcgct ttaatttatc agatgctatc atgctaaaag acaatcacat tgcggcagta
     2401 ggtagtgttc aaaaggcaat tgctcaagcg cgtgcctatg ccccttttgt gaaaatggtc
     2461 gaggtggaag tggaaagcct tgctgctgcc gaagaagctg cggcggcggg tgctgatatt
     2521 atcatgttgg ataatatgtc attggaacag attgaacagg ccattaccct aattgcagga
     2581 cgttctcgga ttgaatgttc tggaaatatt gatatgacca ctattagccg ttttcgtggt
     2641 ttagcgattg attacgtctc cagtggtagt ttaacccata gtgctaagag tcttgatttt
     2701 tccatgaagg gtttaaccta ccttgatgtc taagttgtaa aataaactaa ctttttaaag
     2761 gatgtctttc ctctagaacg agttttatgt cagatagttt aaacgcctct tcaaatatag
     2821 taaaatgaac caaaaatagt acaaaatgag gtataatctt cttatggcat attcaataga
     2881 ttttcgtaaa aaagttctct cttattgtga gcgaacaggt agtataacag aagcatcaca
     2941 cgttttccaa atctcacgta ataccattta tggctggtta aagctaaaag agaaaacagg
     3001 agagctaaac caccaagtaa aaggaacaaa accaagaaag gttgatagag atagacttaa
     3061 aaactatctt actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg
     3121 tcatccaact accatccact atgcgctcaa agctatgggc tacactcgaa aaaaagaacc
     3181 acacctacta tgaacaagac ccagaaaaag tagccttatt tcttaaaaat tttaatagtt
     3241 taaagcacct agcacctgtt tagattgatg aaacaggatt cgatacttat ttttatcgag
     3301 aatatggtcg ctcattaaaa ggtcagttaa gaagaggtaa agtatctgga agaagatatc
     3361 agaggatttc tttggttgca ggtctaacaa atggtgagtt aatcgctcca atgacttacg
     3421 aagagatgat gacgagcgac ttttttgaag cttggtttca gaagtttctc ttaccaacat
     3481 taaccacacc atcggttatt attatggata atgcaagatt ccatagaatg ggtaagttag
     3541 aacttttatg cgaggagttt gggcataaac ttttacctct tcctccctac tcacctgagt
     3601 acaatcctat tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag gtattaccaa
     3661 gttgcaatac cttttatgag gctcttttgt cctgctcttg tttcaattga ctatagttca
     3721 cggatacagt tgggaaagaa gctattaaat atttggagag tccttttggg atgaacatgt
     3781 caaatattac tatagatggg caagaaaaga tgggcaagaa atggatctga gtcaaacgat
     3841 gcttcttatg ctcaagtagt gagtctttat gatgatacaa gtatctctgt tagtaataat
     3901 gagacggaca aagtgttgac tggatccctc tatactgaaa caaatgaaca gggattaacg
     3961 attccaagca gtttactaaa aaattggaat gaacagacag gaaaaaattt gacaactaat
     4021 gatcttattg gcaaatcagt ctcagccagc attgtagaaa gtgctgccga aactagtaag
     4081 attgctcaat ttcaaacgaa gattgtacgt gtaatcaatg atgaagatga catggaggac
     4141 agcaacagtt tcatgctgtc tcatcaaatg gaaacgattt tgaaagaggc tggatttacg
     4201 aaagctgtat cttattttat cttggaactc aaagatccat cacagacaaa agtagtaaca
     4261 gaagaattac agaaaaataa gaagtatact gtgttttctc aacagagagt tcttgatatt
     4321 gtgattacct ttattcgtgt tattcaggga ttattgattg tgctttcatc acaagctatt
     4381 gtggtagcag cggttatgat tggtatcatt atttacatca atatcatgca acgttccaag
     4441 gaaataggtg tcatgaaagc agttggttat cagaatcgtg gtgtcaaagg aatttttatt
     4501 tacgaggcta tctggattgt aggcatcgcc ttgctgctgg catttttggt tgcacaaggg
     4561 gtgggaagtt tggcgaatgc gattgtaagt cacttttacc catccatcac taaggttttt
     4621 gaattaaatc ttttatctgt tttaggaact ctagttttcg ctctattact tggttatgtc
     4681 tcagcctact tcccggcgcg taagattagt aaaatggatc ctgtagaatc gttacgctat
     4741 gaataataag ggattccttg atttacagtc ttaaaaagaa actcacatag aaaaacaatg
     4801 gccatacttg caattcaact caaatagttg tataataggt ttgttgaaag gtgatttgta
     4861 gtgattcaag ttactctttt cacaataaaa atttcatgat tttcataagg aggaaatcac
     4921 taatggtagt taaagttggt attaacggtt tcggacgtat cggtcgtctt gctttccgtc
     4981 gtatccaaaa cgtagaaggt gttgaagtta cacgcatcaa cgaccttaca gatccagtta
     5041 tgcttgcaca cttgttgaaa tacgacacaa ctcaaggtcg tttcgacggt actgttgaag
     5101 ttaaagaagg tggattcgaa gttaacggta aattcgttaa agtttctgct gaacgtgatc
     5161 cagagcaaat cgactgggct actgacggtg tagaaatcgt tcttgaagct actggtttct
     5221 ttgctaagaa agaagcagct gaaaaacacc ttaaaggtgg agctaaaaaa gttgttatca
     5281 ctgctcctgg tggaaacgac gttaaaacag ttgtattcaa cactaaccac gacgttcttg
     5341 acggtactga aacagttatc tcaggtgctt catgtactac aaactgcttg gctccaatgg
     5401 ctaaagctct tcaagataac tttggtgttg ttgaaggatt gatgactact atccacgctt
     5461 acactggtga ccaaatgatc cttgacggac cacaccgtgg tggtgacctt cgccgtgctc
     5521 gcgctggtgc tgcaaacatc gttcctaact caactggtgc tgctaaagct atcggtcttg
     5581 taatcccaga attgaacggt aaacttgacg gatctgcaca acgcgttcca actccaactg
     5641 gatcagttac tgaattggta gcagttcttg aaaagaacgt tactgttgat gaagtgaacg
     5701 cagctatgaa agcagcttca aacgaatcat acggttacac agaagatcca atcgtatctt
     5761 cagatatcgt aggtatgtct tacggttcat tgtttgacgc aactcaaact aaagttcttg
     5821 acgttgacgg taaacaattg gttaaagttg tatcatggta cgacaacgaa atgtcataca
     5881 ctgcacaact tgttcgtact cttgaatact tcgcaaaaat tgctaaataa ttcttgagtt
     5941 gatagaaagc aaggctttgt ggtcttgctt tttatatgga aaaatggatg acacgatcat
     6001 ccattctttt ttaattcttt ttcaaatgta tttgaaaggg tagtgaaagt tagcttctct
     6061 aaagtaagtg ggtgggtaaa ggaaagtcgg aaggcatgaa gcataagccg acttgtcttt
     6121 gatttactat tatagagagg gtcgcccagg ataggaagat tatgatgcga aaggtgcaca
     6181 cgaatctgat gggttcgccc tgtctttagc ttgcaacgag ccaaggaagt cttgtttgag
     6241 aattgcttta acctgcttac atgcgtttca gcatattgcc cattttttgc atcaactatt
     6301 ctttttctac gatcatggcg atcacgtcca attttgtctc tgaaaacaag ttcttttctg
     6361 ttgatatttc catcaactag agcccaatat tctctagaaa tctctttttt ctccaataag
     6421 cgattgagaa tgggcaggat aaaaggattt ttggcaaaga gaactaagcc actggtttcc
     6481 atgtccagac gatgaacgac atagcaggtt tggccaacat aggtactgac atggttaaga
     6541 agggcaattt cgtttggttg attaccatgc gttttcatcc cctctggttt gtttacaata
     6601 atcaagtgtt gatcttgata aacttcctgc actaagtctg ggttgcccca agggatcgtc
     6661 ttttgggaat aatcttcctc gtcaaaagtc aactggcaaa catctccagg atttacgatt
     6721 tcgttccagt ggacttcttc ttgatttatc aaaatatgtt tcttgattct caaaaaatga
     6781 cggatttttc tagggatgag gagttgttcc tcaagtaatt gctttaccgt catttgaggt
     6841 agagaggcgg gtaatgtaaa tgtgaattgc atacagatat tgtaacaaaa aaagccctat
     6901 ttggatagga aatagctaaa ttcttgtctt cctatgatga agatgataaa ataaacgcat
     6961 gaaattagat aaattatttg agaaatttct ttctcttttt aaaaaagaaa caagtgaact
     7021 agaggactct gattctacta tcttacgtcg ctctcgtagt gatcgaaaaa aattagccca
     7081 agtaggtccg attcgaaaat tctggcgtcg ttatcatcta acaaagatta tccttatact
     7141 aggtttgagt gcaggcttgc tagttggaat ctatttgttt gctgtagcca agtcaaccaa
     7201 tgtcaatgat ttgcaaaatg ccttgaaaac tcgaactctt atttttgacc gtgaagaaaa
     7261 agaggctggt gccttgtctg gtcaaaaggg aacctatgtt gagctgactg acatcagtaa
     7321 aaacttgcag aatgctgtta ttgcgacaga agaccgttct ttctataaaa atgacgggat
     7381 taactatggc cgtttcttct tggctattgt cactgctggc cgttcaggtg gtggctctac
     7441 cattacccaa cagctggcta aaaacgccta tttatcgcag gatcaaactg ttgagagaaa
     7501 agcgaaagaa tttttccttg ccttagaatt aagcaaaaaa tatagtaagg agcaaattct
     7561 aaccatgtac cttaacaacg cttattttgg aaatggtgtg tggggtgtag aagatgcgag
     7621 taagaaatac tttggagttt ctgcatcaga agtgagtctg gatcaagctg cgactctggc
     7681 agggatgctc aaggggccgg aactgtataa tcccttgaat tccgtagaag attctactaa
     7741 tcggcgcgat actgtcttgc agaatatggt tgcagcagga tatattgata aaaaccaaga
     7801 aaccgaagct gctgaagttg atatggcttc gcaattgcac gataagtatg aaggaaaaat
     7861 ctcagattac cgttacccct cttattttga tgcggtggtt aatgaagctg tttccaagta
     7921 taatctaaca gaggaagaga ttgtcaataa tggctaccgc atttacacag agctggacca
     7981 aaactaccaa gcaaatatgc aggttgttta tgaaaacaca tcgctatttc cgagggcaga
     8041 ggatggaacg tttgctcaat caggaagtgt agctctcgaa ccgaaaacag ggggagttcg
     8101 tggagttgtc ggtcaagttg ctgacaatga taaaactgga ttccggaatt tcaactatgc
     8161 aacccaatca aagcgtagtc ctggttctac aattaagcct ttagttgttt atacgccagc
     8221 agttgaagca ggctgggctt tgaataagca gttggataac cataccatgc agtatgacag
     8281 ctataaggtt gataactatg cagggatcaa aacgagtcga gaagttccta tgtatcaagc
     8341 cttggcagaa tcgcttaatc tacctgctgt tgccactgtt aatgatttgg gtgtcgacaa
     8401 ggcttttgag gcaggcgaaa aattcggact caacatggaa aaggtcgacc gtgttcttgg
     8461 tgtcgccttg ggaagcggtg ttgaaaccaa ccctcttcaa atggctcaag catacgccgc
     8521 ctttgcaaat gaaggtttaa tgcctgaagc tcattttatt agtagaattg aaaatgctag
     8581 tggacaagtt attgcgagtc ataaaaattc acaaaaacgg gtgattgata agtctgtagc
     8641 tgacaagatg accagtatga tgttggggac tttcaccaac ggtaccggta ttagttcatc
     8701 gcctgcagac tatgtcatgg cagggaaaac tggaacaact gaagcagttt tcaatccgga
     8761 gtacacaagt gaccagtggg taattggtta tactccggat gtagtgatta gccactggct
     8821 tggctttccg accactgatg aaaaccatta tctagctggc tctacttcaa acggtgcagc
     8881 tcatgtcttt agaaacattg ccaataccat tttaccttat acgccaggaa gtacctttac
     8941 ggttgaaaat gcttataagc aaaatggaat tgcaccagcc aatacaaaaa gacaagtaca
     9001 aaccaatgat aatagccaga cagatgataa tttgtctgat attcgagggc gtgcgcaaag
     9061 tctagtagat gaggctagcc gggctatctc agatgcgaag attaaggaaa aggctcaaac
     9121 aatatgggat tcgatagtca atctatttcg ctaagatgct tgtcaaagcc tagctttctt
     9181 gttataatgg ataagatgga ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg
     9241 tttgtggttc gagaaactat tcaatcaaga tcagcggaaa ccccaagcct acacgactag
     9301 aagtaaataa attttgtaag cattgtggca agtacactac acacagagaa acgagatagg
     9361 agagagcgat gcgttttatt ggagatattt ttagacttct taaagacaca acatggccaa
     9421 ctcgcaagga aagctggaga gattttcgtt ctatcatgga atacacagct ttctttgtag
     9481 taattattta catttttgac cagttgattg tttcaggttt gattcgattt attaacattt
     9541 tttagaagat tagtggagtt aattacacta gaaatcttct atttatgaaa ggaaatatca
     9601 tggatagttt tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg
     9661 tgaaagaaaa tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg
     9721 ttgaaattcc aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag
     9781 aaaatcgctt tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg
     9841 ttgttcgaaa cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac
     9901 caactccatt attggaacaa gaaattcgcg acattttggt atctatggga caaactgttc
     9961 aagaatttga tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg
    10021 cagactacac tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta
    10081 tgtttggtaa tgacacagtt gcagaagtaa atctaaacca aatcgcagaa t
//
LOCUS       DAVLTM010000069        10029 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLTM010000069 DAVLTM010000000
VERSION     DAVLTM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.070"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(64..1887)
                     /gene="dnaK"
                     /locus_tag="VYN16_001448"
     CDS             complement(64..1887)
                     /gene="dnaK"
                     /locus_tag="VYN16_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1403963.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2367..2891)
                     /gene="grpE"
                     /locus_tag="VYN16_001449"
     CDS             complement(2367..2891)
                     /gene="grpE"
                     /locus_tag="VYN16_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1403964.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(2918..3952)
                     /gene="hrcA"
                     /locus_tag="VYN16_001450"
     CDS             complement(2918..3952)
                     /gene="hrcA"
                     /locus_tag="VYN16_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1403965.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4116..4553)
                     /locus_tag="VYN16_001451"
     CDS             complement(4116..4553)
                     /locus_tag="VYN16_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1403966.1"
                     /translation="MHLKGWRYRTSRFGLFYFDQCQPDDVIYRIYDSRFLKKYKHELQ
                     DFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMSKGLKWEVMRSRLRSCTTTFLGGLV
                     VCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFRLKRKYRVDE"
     gene            5233..5533
                     /locus_tag="VYN16_001452"
                     /pseudo
     CDS             5233..5533
                     /locus_tag="VYN16_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            5653..7566
                     /locus_tag="VYN16_001453"
     CDS             5653..7566
                     /locus_tag="VYN16_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1403967.1"
                     /translation="MTKEKGLTYEITEAISTPYYTDAFSMKTPRYYQQIAINRTIETV
                     ARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLAKRVLFLADRNILVDQTMAEDFRPF
                     EKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAH
                     RGSAKENSNWRKVIDYFSSATQIGMTATPKETKNASNMEYFGEPIYTYSLKQGIEDGF
                     LAPYRVMRVNLDVDVGGYRPETGKVDANGQLIEDRYYGRKDFDKTIVIDDRTQRVAKF
                     VSDYMKQNNARFDKTIVFCVDIDHAERMRAALVKENLDLVQEDYRYVMQVTGDNAEGK
                     AQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLY
                     PQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLETGAKTVSGSTPGFVDEEGDPVEKY
                     IVTDKQVTILNSTVQVLDENGKLITESLTDYTRKNILGSYATLNDFITVWHTADKKKL
                     ILDELYKKGVYLDAIRESEGISEQEIDDFDLLLKLAYGQKELTKTERINKLKQSGYLY
                     KYSEEARAVLEILLNKYMDKGIGELESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQ
                     AIKELEQELFTVA"
     gene            7579..9042
                     /locus_tag="VYN16_001454"
     CDS             7579..9042
                     /locus_tag="VYN16_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1403968.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9042..>10029
                     /locus_tag="VYN16_001455"
     CDS             9042..>10029
                     /locus_tag="VYN16_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1403969.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT         3154 a         1841 c         1963 g         3071 t
ORIGIN      
        1 ttacaaaaat cattataaac ttttcgtgta ttttttagat actcttcatc caatacactc
       61 gtcttacttt tccgtaaact ctccgtctac gacgtcatcg cctgcgtttc ctgttgcttg
      121 tgcgccttct gctccttctt gagcttgttg cgctgctgcg gcttgttcgt agagtttaac
      181 agcaagtcct tgagcttttt cgttcaatgc ttcaagtttc gctttcatgt cgtccaagtt
      241 gttgtcttct tgagctttct taaggtcatc aagggcagct tgggcagcgt cacgttctgc
      301 gtcgaagcct ttaccttcag tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc
      361 tacttcatta cgaaggtcta cttcttcttt acgtttctta tcagcttcag cgtttgcttc
      421 tgcatctttc atcatgcggt cgatttcttc gtcagtcaaa cctgagttcg attggatgac
      481 aatagtttgt tctttttgag ttccaaggtc tttggcctta acagacacga taccgttctt
      541 gtcgatgtca aatgttactt cgatttgagg aattccacga ggtgcagctg ggatatcagt
      601 caattggaag cgtccaagag tcttgttatc tgctgccatt gggcgttcac cttgaagaac
      661 gtggatatca acggctggtt ggttgtctgc tgctgttgag aagacttgtg atttagatgt
      721 tgggattgta gtgttgcgat cgataagttt tgtaaatact ccacccattg tttcgatacc
      781 aagtgacaat ggcgttacat caagaaggac aacgtccttg acatcaccag taatcacacc
      841 accttggata gccgcaccca tagcaactac ttcatcaggg tttactgatt tgtttggttc
      901 tttaccagtt tcagctttaa cagcttcaac aacggcaggg atacgagttg aaccaccaac
      961 aaggataact tcgtcgattt ctgacaagct caaacctgca tctgaaaggg cttgacgaac
     1021 tggaactttt gtacgttcaa caaggtcacg agtcaaatcg tcaaatttcg cacgagtcaa
     1081 agtcatttcc aagtgaagag gtccagcctc acctgcagtg ataaatggca agctgatttg
     1141 tgttgaagtt acaccagaaa ggtctttctt cgctttttca gccgcatctt tcaaacgttg
     1201 cattgccatc ttgtcagtag acaagtcgat accgttttct ttcttgaatt ctgctaccaa
     1261 gtggtcaatg attttttggt caaagtcgtc accaccaagt ttgttgtccc ctgcagttga
     1321 caatacgtcg aagacaccgt cacccaattc aaggatagag acgtcgaatg taccaccacc
     1381 aaggtcaaat accaagattt tttcttcttt gtcagtcttg tccaaaccat aagcaagagc
     1441 tgctgcagtt ggttcgttaa caatacgttc tacttcaaga ccagcaattt taccagcgtc
     1501 ttttgttgct tgacgttgag cgtcgttgaa gtaagccgga actgtgataa cagctttggt
     1561 tactttctca ccaaggtagt cttcagcgta gcctttcaag tattgaagga tcatagctga
     1621 gatttcttgt ggagtgtatt cttttccatt tgcagaaact ttttcagaag ttcccatctt
     1681 agatttgata gagataactg tatctgggtt tgtgactgct tgacgttttg cagcatcgcc
     1741 aacgataatt tctccgtttt tgaatgagac tacagatgga gttgtgcggt ttccttctgg
     1801 gtttgcgatg attttgcttt cagttccttc aagaactgca actgctgagt ttgttgtacc
     1861 taagtcaata ccgataattt tagacatgtg tttttctcct tgttagttaa ttttctattt
     1921 ttctttttga tactcttctt aagtggcgac gccgtcagag cttgacttcg tcagtctctt
     1981 tgactaaact ttgagcctaa ggtctcaaaa tttgcgcaaa tagcgccacg gcgaagagta
     2041 tcgtctttgg ttcgcttcgc tcactattac aaagcaaaac atattttatc tttcttcata
     2101 gtttattgtc gtttcggaca agttttctat tatgtaaatt gcgacacgag gagtcgaaat
     2161 cgattttatt tcgacgacga gttagtaagg aagctaggca aacgccatag caattgccgt
     2221 tttctgacga gttgctttag ctaccgtcag aattgccgaa tcgaacacgc cctaagctct
     2281 gagtaaaaaa gatagatttt cttgtgttca tcgaacactt cgttaatctc ctatttttac
     2341 tgcgagcttt ttacgggctt tgtatcttag ttatacacca ctaccattgc tgggcgtagg
     2401 atgcggtcat ggagtttgta gcctttttga aagacttggg cgatggtatc tactgggtgt
     2461 tcatcgtctg ctgggagagt ttggatggcc atatggtagt tatggtcaaa ttcgccatct
     2521 gctgcgattt cttcaattcc ttcttctttc aaagcgtgaa tcaagctttc ttgcaccatc
     2581 cccaagccct tcttcacatc atctgtcaaa ccttcaactg caagtgcacg ctcaaggttg
     2641 tcaagagatg gtaagattgc ttttgccaag tcctggctac gataacgttg caagttttga
     2701 cgttcttcat tggcacggcg ttggatattt tgcatttctg catgagcgcg aagatatttg
     2761 ttttcgaact catctgcacg ttcatttgcc aagtccaact cagacttttc aggagttgtt
     2821 tcttcagctg tttccacaac ttcctcttct tgaacttctt ctacttcttc attttttata
     2881 tcttgggcca tgttcgcctc ctttaatgat ttcaatctta atgtacttcg taatgattac
     2941 tgctgaggta gcggtaaaaa tctgtcaact tcatggtcaa aacacggttg accacattga
     3001 cttgattgat tagctgttgg taatccagat taactggacc gataatggct agaattccaa
     3061 ctccccgata aggaatgagg aacttactac taattaccgc taggtcagct aaacaagact
     3121 cttgaccgtc tgcaacacga acattttgca tctgatcctc acgcaacccc tcacgaatct
     3181 ccaaggccac cttttgcggt tggtcaaaga actgataggc tgctagattg gcaaaattca
     3241 agagatggac cttgcccgcc atcacaatgt tttcgttgaa catttcctta aagatgtgtt
     3301 caaagagatc gatgacatta tccgttgttg taaagtaacg ctggataatc tgcggaatct
     3361 ccgtccgaat cttgtagtga atatctaaaa cggtgtgacc gaggaaacgt tcctgaatga
     3421 tgctcttcag tttcagcaaa tcctcctgca agaagttcct tggaatcaga aactgactag
     3481 taaccgttcg cgactcgtct agggtaaata ccgccaaggc tgtatgttgc cccaaaacaa
     3541 cgatatcaaa ggctgtcaaa cgttgcctgc tcggctcaac atccagtgcc actaccgtac
     3601 agccactcag gtctgttaac aagttagcag cctcttgcag aatatcctcc aatttgaaga
     3661 attcctgatc aaaggctttg acaatctcat atacctcatt ttcagccagt cggtcaaaat
     3721 ccagtgagtg tttcacatag tactgaaaac cagcaacact tggcatccga ccgcttgaag
     3781 tatgagcctt ctcaagcaac ccttgttttt ctagttccgc catgtcatta cgaatggttg
     3841 cactgctaga gttaatagac tcttgcaagg cttttgatcc gacaggttcg tgcgttttgg
     3901 taaagatgtc aataatcaga tttaaaatat cctgctgacg ctctgtaacc atctcatcac
     3961 tcctctctgt ctgatcaatt agcactcaat gcatccaagt gctaacttat gattctatta
     4021 tacaccctta aaagagaatg tcaagacaaa aactcaaaaa attagcactc ttttatcaag
     4081 agtgctaaaa atcaatctga agacctagaa ccttgctatt catctactcg gtatttcctt
     4141 tttagtctga aataaccatg aattagataa gaaatcagaa aagcatataa aacaaaaata
     4201 agaaagaaag acatagaaag atcctctcta aacaaactcg tacaaacaac aagaccacct
     4261 aagaaagtag ttgtacagga acgaagtcta gatcgcataa cttcccattt gagcccctta
     4321 ctcatataga cttgatcctc tggaagtaaa tcagaagacg atttacgaag aatcgaacaa
     4381 gaacctgctc ctatcaattc ccaacctcta tctctaaaat cttgcagttc atgcttatat
     4441 tttttaagaa atctagaatc atagatacgg tagatgacat cgtctggttg acattggtca
     4501 aaatagaaca aaccaaaacg actcgttcta tacctccaac ctttcaaatg catctcatgt
     4561 aaatattctt cttacttgtc caaatcaaca atggtgaaaa tccgaaattc tactctgcta
     4621 ttcattgtct taccccaaaa ttagaaaaca tgcctggcgt tatttattag ataattcttt
     4681 ccacttttga ctcaatctcc aaaaaatata agaaatctga atcgcaaaaa ctatcaataa
     4741 aacccaatct attatgaaaa tcaaaaacac tttccaactg aaagaactac ctccagtgac
     4801 aaactttgag aaaaacggta gtagagctaa aaagagaagt aaaataggaa gcatccgcat
     4861 tgttaaaatc cgtttggcat aaaaaaatct ttatttaaac taaaatatta tagcaaaatt
     4921 tacgccagtt tttgaacggc tgatgtagat attttatact ttcaaaatgt ttaaatgtga
     4981 ttatttattt ttgaaaaata gctcaccagc ccgactgaaa gtgcttatag aatgataata
     5041 agtcgcctgc cgaaaacagc gaaaaatagc ggtgttatgc ggagataatc tgacgcgatg
     5101 cgaaagtata ttgcatactt attttcaaca atttagcaga gtatttttat aagtgtgata
     5161 taatagaagt ataatttgtt ctgatagttt attttatgga gaagtagatt tttagaatgc
     5221 ggagggttca atatggttga gtttataaag tctaagaaag aaatgagtga ggaggatatt
     5281 aaagcaaatt tcatcactcc tgctattgta tccaaaggat gaaaaaatgg tgagcatatc
     5341 gcttacgaag aatacttcac tgatggtcga attgaagtta gaggagataa ggctcgtcgt
     5401 aaagaaggaa aaaaatcaga ctattcactg tattaccaat ttggaactcg aattgcaatt
     5461 gttgaggcaa aggataataa acacagcgtt cgagcaggat tacaacaagc tattgaatat
     5521 ggagagattt tagatgttcc atttgtttat tcttcgaatg gtgatggctt tattgaacac
     5581 gaccgtatca cgagagaaga acgtgagctg gagttagacg aattccctac tcgtgaagaa
     5641 ttattttctc gtatgacgaa ggaaaaagga ttgacgtacg aaattacaga agctatctca
     5701 actccatact atacagacgc cttctcaatg aaaacgccac gctattatca gcaaatagct
     5761 atcaaccgta ctattgaaac agttgccaga ggacaaaaac gagtaatgtt tgtgatggca
     5821 acaggaacgg ggaaaacgtt catggctttt caaattattc atcgccttcg aaaagctggt
     5881 ttggctaaac gagttttatt cttagcagat agaaacatct tagtagacca aacgatggct
     5941 gaagacttta ggccattcga aaaggtaatg acgaaaatta caccaaaact tttgactgct
     6001 cctgaaaaat taaattcttt tgaaatttat ctagggcttt atcagcaact aactggtgaa
     6061 gatggaactg aaacacatta tcaaaaattt gacaaagact tctttgattt aatcgtaatt
     6121 gatgaagcgc accgtggttc agctaaggaa aacagtaact ggcgtaaggt aattgattat
     6181 ttcagttctg cgacacagat tgggatgacc gctactccta aagaaaccaa gaatgcttcc
     6241 aatatggaat actttggtga gccaatctat acttatagtt taaaacaggg aatcgaggat
     6301 ggttttttgg ctccatatcg tgttatgagg gttaatttag atgtggatgt gggtggttat
     6361 cgtccagaaa ctggaaaagt tgatgctaac ggacaattaa tagaagatag gtactacggc
     6421 aggaaagatt ttgataaaac cattgtcatt gatgatagaa cgcaaagagt tgccaagttt
     6481 gtttctgatt atatgaagca aaacaatgca cgatttgata aaacaattgt tttttgtgtt
     6541 gatattgacc atgccgagcg aatgcgtgct gcacttgtaa aagagaatct agacttagtc
     6601 caagaagact atcgttatgt catgcaagta actggtgaca acgctgaagg aaaagctcaa
     6661 ctggataact ttatggatgt caattctaat tttcccgcta ttgtaacaac gtctaaatta
     6721 ttaacgacag gagttaatgc taaaacatgt cgtttgattg ttttagactc taatatccaa
     6781 tccatgactg aatttaaaca aattattggt cgtggcacac gtctttatcc tcaaaagggg
     6841 aaagaatttt ttacgattat tgattttcga aatgttacca atttgtttgc tgaccctgat
     6901 tttgatggtg atccagtgaa ggtgctggaa acaggtgcga aaacagtcag tggttctacg
     6961 cccggtttcg tagatgagga aggtgaccca gtagaaaaat atatcgttac agacaagcag
     7021 gttaccattc ttaattctac tgttcaagta ttggatgaaa acgggaaact gattaccgaa
     7081 agcctgaccg actacactcg aaagaatatc ttaggtagct acgccacttt gaacgatttt
     7141 atcacagttt ggcatacggc agataagaag aagcttatct tagacgaact ttataaaaaa
     7201 ggagtttatc tagatgctat tcgagagtcg gagggaatat cagaacaaga aatcgatgat
     7261 tttgatttac tcctaaaact tgcctatggt caaaaagaat taaccaaaac ggaacgtatc
     7321 aataaactca aacaaagcgg atatttatat aaatatagtg aggaagcgcg tgctgttttg
     7381 gaaattttac tgaacaaata catggataaa ggtattggag aactcgaaag cattgaaaca
     7441 ttaaaacttc cagaatttca gatatatggt ggaaccttca aaatcatcaa tacttatttt
     7501 ggagataaaa aacgatattt acaagcaatt aaagaattgg agcaagagct atttacagta
     7561 gcttaatgaa aggaaagtat gtcaattaca tcatttgtaa aaagaattca agatatcact
     7621 cgaaacgatg ctggtgttaa tggtgatgct caacgtattg agcaaatgtc ttggttatta
     7681 ttcttaaaaa tttatgatag ccgtgaaatg gtttgggaat tagaagaaga cgagtatgag
     7741 tcaattatcc cagaggaatt aaaatggcga aattgggctc atgctcaaaa tggggaacgg
     7801 gtattgacag gcgatgaatt acttgatttt gtcaataaca agttattcaa agagttgaaa
     7861 gagcttgaaa taacttcaat tatgcctatt cgaaaaacga ttgttaaatc agcttttgaa
     7921 gatgcgaaca actatatgaa aaatggcgtc ttgttacgcc aagtcatcaa tgttattgat
     7981 gaagttgatt tcaatagccc tgaagatcgt cattcgttta atgatattta cgaaaaaatt
     8041 cttaaagata ttcaaaatgc tgggaactca ggagaatttt atacgccacg tgcagcgact
     8101 gattttattg ccgaagttct taacccaaaa cttggagaat caatggcaga ccttgcttgc
     8161 ggaacaggag gcttcttgac ttcgactctg aaccgtttaa gtagtcaacg taaaactagt
     8221 gaagatacca aaaaatataa tacagctgtt tttggtattg aaaagaaagc atttcctcat
     8281 cttttagcag ttacaaatct gtttcttcac gaaattgatg accctaaaat tgttcatgga
     8341 aatactttgg agaaaaatgt tcgtgaatat acggatgatg aaaaatttga cattattatg
     8401 atgaatccac cttttggagg gtcagaatta gaaacaataa aaaataactt tccagcagaa
     8461 ttacggagtt ctgaaacagc tgatttattt atggctgtca ttatgtatcg tttgaaagaa
     8521 aatggtcgtg ttggagttat tttacctgat ggttttctat ttggtgaagg tgtaaaaact
     8581 cgcttgaaac aaaaactggt agatgagttc aacttgcata cgattattag gttgcctcat
     8641 agtgtctttg caccgtatac aggaatccat acgaacattc ttttctttga taaaacaaag
     8701 aaaacagaag aaacttggtt ttatcgttta gatatgccag atggttataa aaatttctcg
     8761 aaaactaagc cgatgaagtc agaacacttc aatcctgttc gtgactggtg ggaaaatcgt
     8821 gaagagattc tggaaggtaa gttctacaaa tctaaatcat ttacacctag tgaattggct
     8881 gagttgaatt ataatttaga ccagtgtggt tttccaaaag aggaagagga aatcttaaat
     8941 ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca taagattgat
     9001 aatgtattag ctgatatttt gcagttgttg gaggacaaat aatgacacca gaacaactta
     9061 aagcaagtat tctccaaaga gcgatggaag ggaaattagt gccgcaaaat cccaatgacg
     9121 aacctgcaag tgaattatta aagagaatta aagctgaaaa agaaaaactt atcagtgaag
     9181 gaaaaatcaa acgagataaa aaagaaactg agatatttcg tggtgatgat gggaaacatt
     9241 atgggaagtt tgctgatgga agtactcaag aaattgatgt tccttatgat attcctgata
     9301 cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc tctccacgac
     9361 caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa ataggtgata
     9421 ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa tcagggctta
     9481 acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg agttttggta
     9541 gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct atttcgaact
     9601 atgaaaactc attaaataaa gattacctat tctatattct ttcatcaaat gtagtttatt
     9661 ctcaatttct atctctaatt agtggagctg ttgtgaaaaa cttgaatagt gataaagttg
     9721 cttctattct tatccctctc cccccactat ccgaacaaca acgaatagta gaagcaatcg
     9781 aatcagcttt agaaaaagta gatgaatatg ctgaaagtta taatagacta gaacagctag
     9841 ataaagaatt tccagataaa ctaaaaaaat ctattcttca atatgctatg caaggaaaat
     9901 tagttgaaca agacccaaat gatgaatcag tcgaagtttt acttgaaaaa atacgagcag
     9961 aaaaacaaaa actctttgaa gaaggcaaga ttaaaaagaa agatttggac atttctattg
    10021 tttcccaag
//
LOCUS       DAVLTM010000070         9807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLTM010000070 DAVLTM010000000
VERSION     DAVLTM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.071"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            88..1089
                     /locus_tag="VYN16_001456"
     CDS             88..1089
                     /locus_tag="VYN16_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1403970.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1691..1771
                     /locus_tag="VYN16_001457"
                     /pseudo
     CDS             1691..1771
                     /locus_tag="VYN16_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2365..3726
                     /gene="trpE"
                     /locus_tag="VYN16_001458"
     CDS             2365..3726
                     /gene="trpE"
                     /locus_tag="VYN16_001458"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1403971.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3723..4289
                     /locus_tag="VYN16_001459"
     CDS             3723..4289
                     /locus_tag="VYN16_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1403972.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4300..5304
                     /gene="trpD"
                     /locus_tag="VYN16_001460"
     CDS             4300..5304
                     /gene="trpD"
                     /locus_tag="VYN16_001460"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1403973.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5301..6068
                     /gene="trpC"
                     /locus_tag="VYN16_001461"
     CDS             5301..6068
                     /gene="trpC"
                     /locus_tag="VYN16_001461"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1403974.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTR
                     QDAERLAPYFNGILVGTALMQAENVVQRIKELQIDKG"
     gene            6055..6654
                     /locus_tag="VYN16_001462"
     CDS             6055..6654
                     /locus_tag="VYN16_001462"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1403975.1"
                     /translation="MTKVKICGLSTEEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPSDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6632..7855
                     /gene="trpB"
                     /locus_tag="VYN16_001463"
     CDS             6632..7855
                     /gene="trpB"
                     /locus_tag="VYN16_001463"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1403976.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7848..8624
                     /locus_tag="VYN16_001464"
     CDS             7848..8624
                     /locus_tag="VYN16_001464"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1403977.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8596..9255)
                     /locus_tag="VYN16_001465"
     CDS             complement(8596..9255)
                     /locus_tag="VYN16_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1403978.1"
                     /translation="MIDIYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLH
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLFYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASTTGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9325..9771
                     /locus_tag="VYN16_001466"
     CDS             9325..9771
                     /locus_tag="VYN16_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1403979.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
BASE COUNT         2996 a         1821 c         2339 g         2651 t
ORIGIN      
        1 ggatcttaaa taatttacta aattctcttg ttaaaaaaaa ttaaatattg tatagtaata
       61 ttagaatgtt cgtaatggag gactgaaatg aaaaatataa atgggaaaaa ggtaactatt
      121 tatgatattg cacgtttatc aggtttttct ccaaaaacag tttcacgagt tattaatggt
      181 ggagtaaatg ttaaagaaga aacttatcaa gcgattcaaa aagttataga ggaattgtcg
      241 tatattccaa atgcgtatgc aaaaaattta actaaaaaag aagctataaa tatattgatt
      301 tctgtaaaaa aaatcgactc ttttccttta atttggtttc atacgctgtt agataaagtg
      361 ttacggacgt gtaaagaatt tggtgttaat gctatagttg agtactttgg agaggaggat
      421 acaattagta attcaattat atcaagcaca ggtagcctag tagatggtgt tattgttttt
      481 tatgaaagtg tagatgatat cagaattcag tatttaaaga agaaccatat gccttttctt
      541 gttttcggtg aatctcaaac gtctggagta gtctatgtat ctaataataa ttttcaagct
      601 acttatgata tgatgaaagc tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg
      661 ggaggagaat cccatgttaa taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat
      721 aaaaattact ttatggattt aaaagtcatt tatggcttat ctacaattga ttcagtttat
      781 tcttatgcaa tgcagcactt gactacccaa aattatccgg acattatttt tgtttcagga
      841 gatgaaaagg ttcagggctt gattcgtgcg tgctatgaga aaggaattct tattccagat
      901 gatatttcta tcattggatt tgataatatt cctatttctc agtattatac tccagctttg
      961 tctacaattg ctcccaatta tgttaaattg gctaaagaaa tgatagaagg tgttttagca
     1021 attattaaag gggaaagtgt cacatctgtt gaagtttctc ctaaatttgt taggagacag
     1081 agtttctagt attgtttaat tcttgaaaat cctagtggaa aagatggagt gttctaaaat
     1141 aagttctgtt tagcctccat tcagtttcta ttgagttgga tccaatttct aacgagtggg
     1201 acatgttcat gttcgatcta aggaagacat ggtcacattc gaatgatatt ttcaaaaaaa
     1261 tcgtaaaaaa gtcttgacaa agaaaaaaca aagtattaaa ataagttcaa caaatcagaa
     1321 aagtaactat ttttgatctt cagggagcct gtggtgattg tgaataggtg gttggaagta
     1381 gtgaaagtgg gctgattttg aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct
     1441 tacagtgcag cttgttgtta aacaaggcag agatgtaaag ggatagtccc tttataattg
     1501 aggtggcatc gcgttaccaa cgccctcaca cggaagtata ttctgtgtgt gggctttttt
     1561 ctatccgtcg tttggtttat cttttattag ggccttaagt cgctttgatg aacttgagtt
     1621 ctatctacag ctccttgcct actactaaaa gcaaacaaaa aggctggact aatacgaaaa
     1681 aagctagtaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgctc
     1741 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac actgttttga
     1801 ggttgcagat agaactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac
     1861 tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa
     1921 ccatacctac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtataaagg
     1981 tgtaccgaga tgaacatctc ttcatcaatg tctctggtcg tggaggcaag gacctagctg
     2041 cgattgcaga ctactagaag ctaaaaaata atagatggaa aaattacagt tctttctctc
     2101 acagagagct tgtggttgct gaaaacaagc agagaaagct gtaaagttgg gttcatctga
     2161 aacgagagaa gaaaacatca ttctcaaggg agtgcccttt atcgcacagc ctgttacagg
     2221 agatatctga gacaggaatg agagatagga gaaatcctat aattgaggtg gcaccgcgaa
     2281 tttcgtcctc acgcaagtta ttttgcgtgg ggatttttca tacagtcgct acgaactaga
     2341 attagaactg aaagggaaat tacaatggaa cgaatcattc atggagatgt cttatcacca
     2401 atcttggctt atatgcgctt aaaggggcaa cacaaggtta tcttagagag tattccgaga
     2461 gacaaggaaa cagctcgttt ttctatccta gcctataatc ctgtttttga gattaagttt
     2521 gaaaatggag ttctttatca aaatggtcaa gtgattgatc gggatccctt ggatttcctt
     2581 tatgaagtga ttcataagag tcagcaccat tcagagctac cttttggtgg tggagccatt
     2641 ggttttgtgg gttacgatat gatttcgctt tatgaagaaa ttggtcaaat ccctgaggat
     2701 acaattggga cgccagacat gcatttcttt gtctatgaga gctatatagt ctttgaccac
     2761 aagaaggaga agatacatgt catagaggat gcactttata gtgagcgcag tcaagaggcc
     2821 ttggaaaaat ccttgaacca agtgcttgag gaattacgca ttcctgctcc aaatgaattt
     2881 gaagacttgg atttatcacc acttgacttc aaaccgcata tcgctccaca taagtttgag
     2941 gtaatggtag aaactgctcg cgacttgatt cgtaatggtg atatgttcca atgtgtactc
     3001 agccagcgtt tctcagcaga agttactgga aatccatttg acttctacag aaatctccgc
     3061 gtgactaatc cttctaatta cctttatttc tatgattttg gggattatca aatcatcgga
     3121 gctagtccag aaagtttggt ttctgtcaaa aatggcatcg tgacaaccaa tccgattgca
     3181 gggacgcgac caagaggggc tacggatgaa gaagacaagg ccttggcgac tgacctcctc
     3241 tctgatgaga aggaaacagc agaacatcga atgttggtag acttggggcg taacgatatt
     3301 ggccgcatct ctgaaacggc cagtgtccaa gttactaagt atatggaggt ggagctcttc
     3361 cgctacgtca tgcatttgac cagtgtggtc aagggacgtt tgcttgcaga actcactgcc
     3421 atggatgcct tgaaagcaac gcttcctgct ggaaccgttt ctggagcacc aaaaattcga
     3481 gctatgagac gcatctatga actggaaacg gaaaaacggg gcgtatatgc aggagcaatc
     3541 ggctacttgt ctgcgacggg tgatatggat ttggccattg ccatccgaac catgattctc
     3601 aaaaatcaaa gagcctatgt gcaggctggg gcagggattg tctacgactc tatcgcccaa
     3661 aacgaatacc aagaaaccat taacaaagca aaatctatga ctagaattgg agaactaaga
     3721 ccatgatttt attgattgac aactatgatt cttttaccta taacttggcc caatacattg
     3781 ggaattttgc agaagttcag gttctgagaa atgatgattc caagctgtat gaagaagctg
     3841 aaaaagcaga tggtctggtc ttttctcctg gtcctggttg gccagttgat gctggaaaga
     3901 tggaagacat gatttgtgat tttgcaggca agaagccgat tctagggatt tgtttgggtc
     3961 accaagccat tgcagaagtc tttggtggta agctaggttt ggctccaaaa gtcatgcatg
     4021 ggaaacagag caatatcaac tttgaagcgc catctgtttt gtatcaaggt attgaggatg
     4081 gacgtgcggt catgcgttat cacagtattt tgattgagga aatgccagaa gactttgaag
     4141 tgacagctcg ttcgactgat gaccaagcca tcatggggat tcaacataaa aacctaccga
     4201 tttatggctt ccagtaccat ccagagagca ttggaacgcc agacggcttg tcttctattc
     4261 ggaattttat cgagaaggtt gtaaagtgag gaaactagga tgaaagagat tattgaaaaa
     4321 ctagcaaaat ttgaaaattt atcaggtgtg gaaatgacgg atgtcattga gcgtatcgta
     4381 actgggcgtg taactgaagc gcagattgct tctctcctct tagctctcaa gatgaagggg
     4441 gaaacacctg aagaacgcac agccattgcc caagtcatga gaggacatgc ccagcatatt
     4501 ccaactgaga ttcatgatgc catggataac tgtggtacag gtggggacaa gtctttcagt
     4561 tttaatattt ccacaactgc agcctttgtc ttggctggtg gcggtattca catggccaag
     4621 catggtaacc gctcgatttc ttctaaatct ggttccgcag atgtcctcga agccttggga
     4681 atcaatcttg atctcaaacc agctgaacta ggtaaggtct ttgataaaac tggaatcgtc
     4741 tttctcttcg ctaaaaatat gcacccagct atgaaataca tcatgccagc tcgtttggaa
     4801 ttgggaattc caacgattat gaacttgact ggtccactga ttcatccaat ggctttagaa
     4861 acacagcttc ttggaattag tcgtccagaa ctcctagaaa gtacagctca ggttttgaaa
     4921 aatatgggtc gcaaacgtgc catcgtggtt gctggaccag aagggttgga tgaagctggc
     4981 ttgaacggaa caaccaagat tgcacttctt gaaaatggcg aaatcagctt gtcaagcttt
     5041 actccagagg atttgggaat ggaaggctat gctatggaag atattcgtgg tgggaatgct
     5101 caggaaaatg cagaaatttt gcttagcgtt ctgaaaaacg aagcaagtcc attcttggaa
     5161 acgacagtct tgaatgctgg tcttggtttc tatgctaatg gtaagattga tagcatcaag
     5221 gaaggagttg ccttggcccg tcaagtgatt gctagaggca aggcccttga aaaactcaga
     5281 ctgttacagg agtaccaaaa atgagtcagg aatttttagc acgaatctta gagcagaagg
     5341 cgcgtgaggt ggagcagatg aagctggagc aaatccagcc tctgcgccag acctatcgct
     5401 tggcagaatt tttgaagaat catcaggacc gcttgcaggt aatcgctgag gtcaagaaag
     5461 ctagccctag tttgggagat atcaatctcg atgtggatat tgtgcaacag gcccagactt
     5521 atgaagaaaa cggagcagtg atgatttcgg tgttgacaga tgaagttttc tttaaaggac
     5581 atttggatta tctacgggag atttccagtc aggtagagat tccgacgctc aacaaggact
     5641 ttatcatcga tgaaaagcaa atcatccgcg ctcgcaatgc aggtgcgaca gtcatcttgc
     5701 tcattgtggc agccttggca gaagaacgtc ttaaggaact ttacgactac gcgacagtgc
     5761 ttggtctgga agtcttggtg gaaactcaca atctagctga actagaagtg gctcacagac
     5821 ttggtgccca gattatcggg gtcaataacc gcaacttgac cacctttgag gtcgacttgc
     5881 agaccagtgt agacttggct aaacatttca aagatgattg cttgtacatt tctgaatctg
     5941 ctattttcac aaggcaggat gcggaacgac tagccccata ctttaacgga attttggtag
     6001 ggacagctct tatgcaggca gaaaatgtgg tccagagaat caaggagttg cagattgaca
     6061 aaggttaaaa tttgtggact atcgaccgaa gaagcggtgg aaacagccgt ttcagcagga
     6121 gccgactaca tcggttttgt ctttgcacct agtaaaagac aggtgacttt agaagaggca
     6181 gctgagttgg caaagcttat tccttcagat gtgaaaaagg ttggagtatt tgtttcacca
     6241 agtcgggtag aactgctgga agcgattgac aaagttggct tggacttggt tcaagttcac
     6301 ggtcaggtgg cagatgattt atttgagaat ttgccttgtg ccagtattca ggctgtgcag
     6361 gtagatggaa atgggcatgt ccccaattct caggcagatt atctactctt tgatgcccct
     6421 gtggcaggaa gtggccagcc ctttgattgg ggtcaactgg atacgactgg actagcacag
     6481 cccttcttta tcgcaggtgg ccttaatgaa gataatgtag taaaagcaat tcaacatttt
     6541 actccctatg cagtagatgt atcgagcgga gtggagacag atggacaaaa agatcatgaa
     6601 aagattagaa gatttataga gagggtaaag catggcatat caggaaccaa ataaagatgg
     6661 attttacgga aaattcggcg gacgttttgt cccagaaaca ttgatgacag cagttttgga
     6721 gttggagaag gcctatcgtg aaagtcaggc agacccaagt ttccaagagg aattaaacca
     6781 actcttgcgc cagtatgtgg ggcgggaaac tcctctttac tacgcaaaaa acttgaccca
     6841 gcatattggc ggagccaaga tttatctcaa acgggaagac cttaaccata caggagccca
     6901 caagattaac aatgccttag gacaagtttg gcttgccaaa cgcatgggta aaaagaaaat
     6961 tatcgcagaa accggtgctg gtcagcacgg tgtggcaact gcaactgctg cggccctctt
     7021 taacatggaa tgtaccatct acatgggtga ggaagatgtc aaacgccaag ccctcaatgt
     7081 cttccgcatg gagcttttgg gagccaaggt cgaggctgta acagatggtt cgcgcgtgct
     7141 caaggatgcg gtcaatgcag cccttcgttc atgggtggct aatatcgacg atacccacta
     7201 tatccttggt tctgccttgg ggcctcatcc atttccagaa atcgttcgtg acttccaaag
     7261 tgtcatcggt cgcgaagcta aacaacagta tcgcgatatg acaggtcaaa atctgcccga
     7321 tgctctagta gcctgtgttg gtggtggatc taatgctatt gggctcttcc atccctttgt
     7381 agaagatgag tcagtagcta tgtatggaac tgaagcagca gggcttggtg tggatacaga
     7441 gcaccacgct gctaccttga ccaagggtcg tccgggtgtt cttcacggtt ctctcatgga
     7501 tgtgctccaa gatgcccatg gtcaaattct tgaagccttc tctatctcag caggtttgga
     7561 ctatcctggt attggtccag aacattctca ctaccacgat atcaaacgtg ccagctatgt
     7621 ccctgtgaca gacgaagaag ccttggaagg attccaactc ttgtctcgtg tggaagggat
     7681 tatcccagcc ttggaatcta gccatgctat cgcttttgcg gtgaaattgg ccaaagaact
     7741 tggaccagaa aagtctatga ttgtctgcct atcaggtcgt ggggacaagg atgtggttca
     7801 agtcaaagac cgcttggaag cagatgcagc aaagaaggga gaagctcatg cctaagacac
     7861 taacagaaaa attgaatgct attaaagcag ctggaaaagg aatttttgtt ccctatatca
     7921 tggctggtga ccacgagaaa ggtttggacg gtcttgctga aacaattcac tttttagaag
     7981 atttgggtgt ctctgcaatt gaagtgggta ttcccttttc agaccctgtt gcagatggac
     8041 ctgttatcga agaagctggc ttgcgcagtt tagcccacgg gacctctacc caggctttag
     8101 ttgaaacctt gaaaaccatt gaaacagaga ttccactggt catcatgacc tacttcaacc
     8161 ccctctttca gtacggtgtg gagaactttg tcaaagatct ggctgataca gctgtgaagg
     8221 gcttgattat cccagacttg cctcatgaac atgccaactt tgtagaacca tttttggcag
     8281 atacagatat cgccttgatt cctttagtaa gcttgaccac aggaattgag cgccagaaag
     8341 agttgattga aggggcggag ggattcatct atgccgttgc catcaatgga gtgacaggga
     8401 aatcaggcaa ttaccgtgca gatttggaca agcacttggc gcaacttcat caagtggccg
     8461 acatcccagt cttgacaggt tttggtgtat ctagtcaagc cgatctggaa cgcttcaatg
     8521 cggtgtcaga tggtgttatc gtcggttcga aaatagtaaa agctctccac caaggagagc
     8581 cgattcagga ctttatcaaa caagcagtag cttaccaaaa ataatcaaac aagtagcaag
     8641 taagaggaaa ggcacgaaag gaagtctttc ctttttcttt tgcaggagaa aggccaggat
     8701 acccgtcgta gaagcgaact gaatcaagat cagtaactcc gttacgctaa agacgagagc
     8761 acaagaagct aaaaaaagga aatcccctgc acccatgcgg atatcgataa aatgagccaa
     8821 aattccaagt atgaggaagg agaccatgac cagattccag ccagaggaag ctattaggat
     8881 taggtggaaa gtcatccaga ccagtaaggg atattcctga tggtgaaagt cgtagatacc
     8941 caaggtcaaa ccagcggtga ttaggacgac ttgccccaag gaaagcaatt cccaagagta
     9001 aaacagaaag aggagtccta agcctagttc aaagagggca taccagacag gatagcgaac
     9061 tttgcagtag cgacagcgaa agcgattgaa gacctgtgag agaatcggaa tcaaatctaa
     9121 gggatgcaag cgagtctgac aggaatcgca gtgactggct gaactgataa tggattgctc
     9181 tggaaaacgg tcaatgacca aaccaagaaa ggaagcgaga atgctcccga caagaaaaaa
     9241 ataaatatca atcatactta tctattcgta aaaaatggga gaagtagtat aatggagaaa
     9301 catagataag atggggagga aaagatgaca attcgttttg aagaaaaggt gagtatagaa
     9361 aacgctcagc tcgtatgcca gtggtccaac tccttaggca aagtctttca agaacaatgg
     9421 atgggaccaa ggattccttt tccactgacc cttcaagcct tggaaggagt cttttcaatc
     9481 tttgatgaac aagagtttgt gggacttatc cagaaaatca ggctagaaga cagcaatctt
     9541 catatcggga gattttttat caacccccag aaacaggggc aaggcttaga tagccaagct
     9601 ttaaggaaat ttgttagttt ggcctttgaa aatgaagata tagatagtat ttctctaaat
     9661 gtcttcgagg caaatcaaag agttcagaat ctttaccaaa aagaaggatt tgaaatcgtt
     9721 caaatggttg aagcacctgt acgaaaatat agtagattga aactagaata gtacacctct
     9781 acttctaaaa cattgttaga aatcgat
//
LOCUS       DAVLTM010000071         9399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLTM010000071 DAVLTM010000000
VERSION     DAVLTM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.072"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            111..1490
                     /gene="glmU"
                     /locus_tag="VYN16_001467"
     CDS             111..1490
                     /gene="glmU"
                     /locus_tag="VYN16_001467"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1403980.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1500..2045
                     /locus_tag="VYN16_001468"
     CDS             1500..2045
                     /locus_tag="VYN16_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1403981.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            2057..2371
                     /locus_tag="VYN16_001469"
     CDS             2057..2371
                     /locus_tag="VYN16_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1403982.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2388..3080
                     /locus_tag="VYN16_001470"
     CDS             2388..3080
                     /locus_tag="VYN16_001470"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1403983.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVCGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3157..3648
                     /gene="rocS"
                     /locus_tag="VYN16_001471"
     CDS             3157..3648
                     /gene="rocS"
                     /locus_tag="VYN16_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1403984.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3746..4336
                     /locus_tag="VYN16_001472"
     CDS             3746..4336
                     /locus_tag="VYN16_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1403985.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIDYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            4345..6108
                     /locus_tag="VYN16_001473"
     CDS             4345..6108
                     /locus_tag="VYN16_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1403986.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVITCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQENPEYTDLLLKKALEFFEF"
     gene            6322..6837
                     /locus_tag="VYN16_001474"
                     /pseudo
     CDS             6322..6837
                     /locus_tag="VYN16_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7007..7207
                     /locus_tag="VYN16_001475"
     CDS             7007..7207
                     /locus_tag="VYN16_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403987.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKVPEDSALTE"
     gene            7232..7648
                     /locus_tag="VYN16_001476"
     CDS             7232..7648
                     /locus_tag="VYN16_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403988.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGLAETETKLVEELKAILDKLK"
     gene            7879..8586
                     /gene="ccdA2"
                     /locus_tag="VYN16_001477"
     CDS             7879..8586
                     /gene="ccdA2"
                     /locus_tag="VYN16_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1403989.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            8597..9154
                     /gene="sdbB"
                     /locus_tag="VYN16_001478"
     CDS             8597..9154
                     /gene="sdbB"
                     /locus_tag="VYN16_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1403990.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
BASE COUNT         2961 a         1631 c         2001 g         2806 t
ORIGIN      
        1 tcaaaaaaat gggttaagga agagacttta gagcattttt tcattcaaga gtgcggaatg
       61 atttgaaata tggtataata aaagggaatt tctacagaaa agagaagatt atgtcaaatt
      121 ttgccattat tttagcagcg ggtaaaggga ctcgcatgaa atctgatttg ccaaaagttt
      181 tgcacaaggt tgcgggtatt tctatgttgg aacatgtttt ccgtagtgtg ggagctatcc
      241 aacctgaaaa gacagtaaca gttgtaggac acaaggcaga attggttgag gaggtcttgg
      301 ctggacagac agaatttgtg actcaatctg aacagttggg aactggtcat gcagttatga
      361 tgacagagcc tatcttagaa ggtttgtcag gacacacctt ggtcattgca ggagatactc
      421 ctttaatcac tggtgaaagc ttgaaaaact tgattgattt ccatatcaat cataaaaatg
      481 tggccactat cttgactgct gaaacggata atccttttgg ctatggacga attgttcgta
      541 atgacaatgc tgaggttctt cgtattgttg agcagaagga tgctacagat tttgaaaagc
      601 aaatcaagga aatcaacact ggaacatacg tctttgacaa cgagcgtttg tttgaggctt
      661 tgaaaaatat caataccaat aacgctcaag gcgaatacta tattacagac gtcattggta
      721 ttttccgtga aactggtgaa aaagttggcg cttatacttt gaaagatttt gatgaaagtc
      781 ttggggtaaa tgaccgtgtg gcgcttgcga cagctgagtc agttatgcgt cgtcgcatca
      841 atcataaaca catggtcaac ggtgttagct ttgtcaatcc agaagcaact tatatcgata
      901 ttgatgttga gattgctccg gaagttcaaa tcgaagccaa tgttaccttg aaagggcaaa
      961 cgaaaattgg tgctgagact gttttgacaa acggtactta tgtagtggac agcactatcg
     1021 gagcaggagc ggtcattacc aattctatga ttgaggaaag tagtgttgca gacggtgtga
     1081 cagtcggtcc ttatgctcac attcgtccaa attcaagtct gggtgcccaa gttcatattg
     1141 gtaactttgt tgaggtgaaa ggatcttcaa tcggtgagaa taccaaggct ggtcatttga
     1201 cttatatcgg aaactgtgaa gtgggaagca acgttaattt cggtgctgga actattacag
     1261 tcaattatga cggcaaaaac aaatacaaga cagtcattgg agacaatgtc tttgttggtt
     1321 caaattcaac cattattgca ccagtagaac ttggtgacaa ttccctcgtt ggtgctggtt
     1381 caactattac taaagacgtg ccagcagatg ctattgctat tggtcgcggt cgtcagatca
     1441 ataaagacga atatgcaaca cgtcttcctc atcatcctaa gaaccagtag gagcctatca
     1501 tggagtttga agaaaaaacg cttagccgaa aagaaatcta tcaaggacca atatttaaac
     1561 tggtccaaga tcaggttgaa ttaccagaag gcaagggaac tgcccaacgg gatttgattt
     1621 tccacaatgg ggctgtctgt gttttagcag taacggatga acaaaaactt atcttggtca
     1681 agcagtaccg caaagctatc gaggctgtct cttacgaaat tccagccgga aaattggaag
     1741 taggagaaaa cacagcccct gtggcagctg cccttcgtga attagaggaa gaaacagcct
     1801 atacagggaa attagaactc ttgtacgatt tttattcagc tattggcttt tgtaatgaga
     1861 agttaaaact atatttagca agcgatttga caaaagtgga aaatccgcgt ccgcaggatg
     1921 aggatgaaac cttggaagtc cttgaagtga gcttagaaga agcgaaagaa ttaatccaat
     1981 caggtcatat ttgtgatgcc aagacaatta tggctgttca gtattgggag ttgaagaaaa
     2041 aatagaggag gtcagtatgg gtaaatcttt attaacggat gaaatgattg aaagagctaa
     2101 tagaggcgaa aaaatttcag gtcctccttt gctagatgat aatgaggaga ctaagatttt
     2161 accaacctct tcttcccgtt ttggttatgc caatcctaag aatcatggtt ttagccagga
     2221 aaccttgaag attcaggtcg aaccatctat tcataaaagc cgtcgcattg aaaataccaa
     2281 gagaaatgtc ttcaattcta agttgaataa aatcttattc gcggtcatct ttctcttgat
     2341 tttgcttgtt ttagcaatga aacttttgta atagaaaagg aattgaaatg aaaataggaa
     2401 ttattgctgc tatgccagaa gaactggctt atctggtcca gcatttagat aatgcccagg
     2461 agcaagttgt ttgtgggaat acctatcata caggaaccat tgcttctcat gaagtcgttc
     2521 ttgtagaaag tggaattggt aaggtcatgt ctgctatgag tgtggcgatt ttggctgatc
     2581 atttccaggt ggatgccctt attaatacgg gttcagctgg ggcagtagca gaaggtatcg
     2641 ctgttgggga tgtcgtgatt gctgacaaat tagcctatca tgacgtggat gtcacagctt
     2701 ttggctatgc ttatggacaa atggcgcaac aaccgcttta tttcgaatca gacaaaacct
     2761 ttgttgctca aatccaagag agtttatctc aattggacca aaactggcat cttggtttga
     2821 ttgctacagg agatagtttt gttgcaggaa atgacaagat agaagcgatt aagtcccatt
     2881 tcccagaagt tttagccgtg gagatggagg gggcagctat tgctcaagca gcgcatgccc
     2941 tcaatctccc agtcttagtc atcagagcta tgagtgacaa tgccaaccat gaagcaaaca
     3001 tcttttttga tgagtttatt atcgaagctg gacgtcgctc tgcccaagtc ttgttgacct
     3061 ttttgaaggc tttagattaa gcggaaattt gacagttttt ctagcttatg ataagattta
     3121 agtaaagaaa agctagaaaa cgtttcagag gatattatga gtattgaaat gaccgtcagt
     3181 gagattgcag aggtcttagg attatctcgc caagcaatca ataaccgtgt caaagaatta
     3241 ccagaagaag acacagataa aaatgacaaa ggggtaacag ttgttaccag aagtggcttg
     3301 attaagctag aagaaatcta taaaaaaacg atttttgaag atgagcctgt cagtgaagat
     3361 gtcaaacaac gtgaactgat ggagattcta gtggatgaga agaatgcaga aattcttcgt
     3421 ctttatgaac aattaaaagc caaggatcgt cagttatcag aaaaagacga gcagatgcgt
     3481 atcaaagacc gtcagattgc tgagaaagac aaacaattag atcagcaaca acaattgacc
     3541 cttcaagcca tgaaggatca agaaaatctt aagctagagc tggaccaagc aaaagaagaa
     3601 gtccaatcca ctaagaaagg cttttttgct cgtttatttg gaggataatc aaggagctgt
     3661 ttaggttaaa tgctaacctg atggcttctt ttgtttgtga atagtatagt tgattagaac
     3721 agagacagta gaggagagga acagaatgga acgattagaa gattacattg cttttgactt
     3781 agaatttaat cagcacgaag gacaaacaca cttgattcaa gtatcggcag tgcgttttag
     3841 agatggtcgg gaaatcgatg cctatgattc ctatgttcat accagcgtcc ctctaaaaag
     3901 ctttatcaat ggtttgaccg ggattacagc tgagacctta aaagatgctc ctccagttga
     3961 gaaagtctta gaagaattcc aagagtttgt gggttctttg ctgatgatag actacaatgc
     4021 tgctaaaagt gatttaccta ttttagcaga gcatggttta gactatagcc aacagtacaa
     4081 ggtggatttg tatgatgaag cttttgaacg tcggagttca gatttacatg gtattgccaa
     4141 tctcaaattg caaactgttg cctcatttct tggatttcaa ggtcagtctc ataatagttt
     4201 agaggatgct cggatgacgg cgcgtgttta cgaatccttc ttagagtcgg atcaagctag
     4261 ggaattgtta gagacagaaa gtagcttatc aaacaatcct tttggtgtct tggacttgtc
     4321 tcaattattt gactaggagt gcctatgaaa cctgaaaaac ctaaaaaatt gggtcttaaa
     4381 cagatttacc tcaatcttga taaaatcctc tttctatttt tcttgatttt tatgatagtg
     4441 gagtatttgt gggtaccatt caattcatgg ttggctagct tcttattaga tcagacaggt
     4501 tatcttttta tttcctataa caatgtctta gccatcctta ctcattctcc attgattagt
     4561 ctagcttttt tagccttgat tgtaattaac cttctggttg cttattttca aataggactc
     4621 ctctttattg gtgctcgtca tctcctctat aatgaaaagc gaaggctaat tgagtatatc
     4681 cgcaaggttt tccatgaaag ttttggattt atgaaaaagc taactggcgc taaagccttg
     4741 tttatctttt tctatatagc tatgcttttt ccttttatac ggaaaatgct gaagatttat
     4801 tatcttaata aaatcgtcat tccagagttt atccaagctt atttagaaga taaatattgg
     4861 atgtggtggc tgagcatcct acttctatct cttatctttc tctatgtctc tgtcagattg
     4921 atgtttgccc ttccgaagat tgtatatgat cagctgactg ttcgagaagc agtgatgttt
     4981 agcttggaga aaaccaagaa gagagttact ttttatgctt ggaatttatt ttacatcttg
     5041 ctcaaagcac atttattatt ttatcttcct ttgattcctt tattattggc tcaaactttg
     5101 gtagatgata tcactcagag agagtctctg attcttggta ttttgaattt tgtcgtgatt
     5161 aaaaatctct attatatggc tctgacttat tttttggtta aatttgtttc atttttgaca
     5221 ggaaaggagc tggatatgct tcctaggaga gaaaaagatc acatcgtgcg atggggtgtc
     5281 ataacttgtg ccagtctttt ctttgcctta gaaggttata tttatctgga ggctcccatg
     5341 gttcatctac ctcaacttat ttctcaccga ggggtttcca atgcaaatgg gattcaaaat
     5401 acagtagagt ccttggaaac tacagcacaa ctcaaaccag acttggtgga gacggacgtg
     5461 caggaaacaa aagatgggca gtttgtcatg atgcatgatg ctaacttgaa aaatctagca
     5521 ggtatcaata aaagtcctca agacttaaac ttggaggagc ttaaagggat tgatattttt
     5581 gaaaatggct accagactaa aatttcaagc tttgaagatt atctcagtcg agccaacgaa
     5641 cttggtcaaa aattactaat tgaaattaaa accagtaaaa aagatagtcc agacatgatg
     5701 aaccgctttt tagcccgtta tgctgcaaag ctcaagattt atggacatca aatccagtct
     5761 ttagactacc atgttgtcga aaaagtaaga cagtatgatg ccgaactgcc agtttatttc
     5821 atcatgccct acaattctgt ctttcctaaa acaagagcga caggatatac tatggagtac
     5881 tcaaccttgg atgaatattt tgtaagcaaa ctatggacaa cggatcagaa actttatgtc
     5941 tggactgtca atgattcaga agctatcagc aaatctcttc acttaggagt agatggagtg
     6001 attacagatg atttggaaaa agttcagcgg gaaatagaag tagcccaaga aaatccagaa
     6061 tatacggatt tgctcttgaa aaaagcattg gaattctttg aattttagta ataaaaaaga
     6121 ggaacggttg ttcctcccat gataggtaag tattcttgtg aatgcttgct tttttgtggt
     6181 tgcaatccgt ttacgagaat tgtttgtatt gttcttgttt caatttactt tatagtgctt
     6241 tgaagttgga atgctacact ataacttcta aaatatagta aaatgaacca aaaatagtac
     6301 acaatgtggt ataatctttt tatggcatat tcaatagatt ttcgtaaaaa agttctctct
     6361 tattgtgaac gaacaggtag tataacagaa gcatcacacg ttttccaaat ctcacgtaat
     6421 accatttata gctggttaaa gctaaaagag aaaacaggag agctaaacca ccaagtaaaa
     6481 ggaacaaaac caagaaaagt tgatagagat agacttaaaa actatcttac tgataatcca
     6541 gacgcttatt tgactgaaat agcttctgaa tttggctgtc atccaactac catccactat
     6601 gcactcaaag ctatgggcta cactcgaaaa aaagaaccac acctactatg aacaagaccc
     6661 agaaaaagta gccttatttc ttaagaattt taatagttta aagcacctag cacaggtcta
     6721 acaaatggtg aattaatcgc tccaatgact tacgaagaga tgatgacgag cgactttttt
     6781 gaagtatggt ttcagaagtt tctcttacca acattaacca caccatcggt tattatagcg
     6841 aaatgaaata agaacaggac aaatcgatca ggacagtaaa atcgaattct aacaatgttt
     6901 tagaagtcca tatgtactat tctagtttca atataccata actgttccta gtcaacctgc
     6961 aagtcctgtc ccagctccaa gctcagctcc gactcaagat cctagagtgg tagcaagcgc
     7021 ttcggctagc tcaacgagca cacaagctca agagcaagtt gacaagtctg aacttcgtgc
     7081 cttgagtcaa gagttagacc aacgcttgaa agctttggca acagtatctg atccgaaaat
     7141 cgatgcaacc aaggctgtcc tcctagatgc tcaaaaagtt ccggaagata gtgccttgac
     7201 agagtaaggt ctgcatactg cagtagagtc tgtgaaggct gccttggact ctctcaaaga
     7261 tgtgaaagca aatgctagcg acagcaagcc tgcacaggac aagaaggatg caaaacaagg
     7321 aacggaagat agtaaggatt cagataagat gactgaaaca aactcagttc cggcaggagt
     7381 gattgtggtc agtctacttg ccctcctagg cgtgattgcc ttctggctga ttcgccgtaa
     7441 gaaagagtca gaaatccagc aattaagcac ggaattgatc aaggttctag gacagctaga
     7501 tgcagaaaaa gcggataaaa aagtccttgc caaagcccaa aaccttctcc aagaaaccct
     7561 tgatttcgtg aaagaagaaa atggcttagc agagacagaa actaaactag tagaggagct
     7621 taaagcaatc cttgacaaac tcaagtaaaa catgatttat gaaatgtcag acctaaaaga
     7681 cattttcgtt cacaaaagca gcgatgagta atccttgtcg ttgctttttg gctattttgg
     7741 gggataagat ccgccttgac tagcaaaaga aaagattggt ccaatagtgc ccgatacgat
     7801 tttgcgtagc agaaagctta caaataaaat tgaatatagt aaaataggat tagaattatt
     7861 aagaaagttg gttctttatt ggaaacgata gtatttttaa tctctgtttt tctagcaggt
     7921 gttttatcct ttttttctcc ttgtattttt cctcttctgc cagtctatgc tgggatttta
     7981 ttggatgatc aagaaagtgc aaaaagcttt tctttgtttg ggagaaaggt tctctggtca
     8041 ggcttgattc gaacactttg ctttatcgct ggtatctctc tcattttctt tattctaggc
     8101 tttggtgctg gttactttgg tcatattctc tatgcaaatt ggtttcgata tggcatggga
     8161 gctattatta tcattttggg tcttcaccag atggaaattt ttcatttgaa gaaattagaa
     8221 gttcaaaaaa gttttacctt taaaaaatca gattctaatc gttattggtc agctttttta
     8281 cttggtatta cctttagctt tggttggaca ccttgtattg gtccagtttt aagttctgtt
     8341 ttagcacttg cggcttctgg aggcaatggc gcttggcaag gagcgattta tactctcatt
     8401 tacactctgg gcatggccct tcctttcttg gtattggcac tagcttcagg tctagtcatg
     8461 ccatatttta gtaaaatcaa gcgtcatatg atgctactaa agaaaattgg tggtttcctc
     8521 attgttttaa tgggaatttt gttactatta ggacaagtaa atgttctagc tggaattttt
     8581 gaataaagga ggacaaatga aaaaagtaat gtttgctggc ttaagtctct tgtcattagt
     8641 tgtattgatg gcctgtggtg aggaagaaac taaaaagact caagcagcac aacagccaaa
     8701 acaacaaacg actgtacaac aaattgctgt tggaaaagat gctccagact tcacattgca
     8761 atccatggat ggcaaagaag ttaagttatc tgattttaag ggtaaaaagg tttacttgaa
     8821 gttttgggct tcatggtgtg gtccatgcaa gaaaagtatg ccagagttga tggaactagc
     8881 ggcgaaacca gatcgtgatt tcgaaattct tactgtcatt gcaccaggaa ttcaaggtga
     8941 aaaaactgtt gagcaattcc cacaatggtt ccaggaacaa ggatataagg atatcccagt
     9001 tctttatgat accaaagcaa ccaccttcca agcttatcaa attcgaagca ttcctacaga
     9061 atatttaatt gatagccaag gaaagattgg aaagattcaa tttggtgcta tcagtaatgc
     9121 ggatgcagaa gcagcattta aagaaatgaa ctagaatcga taaaatctga ttacaagatg
     9181 tagtcagatt ttggtaactt ctaaaactaa cttccagttt cccacaacct agcttttagt
     9241 atgagaaaaa cgcgtgacat cagtggaaat ccgtcttaga ctaacttcca ctggttttct
     9301 ttttcttgat atataagggt tcaaaagcga aaagactcag aaaaagtgca cgacaaatag
     9361 ccctaaaaca gagtcgtctt agagtaactt ccagttttg
//
LOCUS       DAVLTM010000072         9381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLTM010000072 DAVLTM010000000
VERSION     DAVLTM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.073"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            2..1045
                     /locus_tag="VYN16_001479"
     CDS             2..1045
                     /locus_tag="VYN16_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403991.1"
                     /translation="MKKEIPVLDSSTDYWLVRANSGEYYTDFNLNGYIGIGWNEITLE
                     DIRRADNNSNVLKEILKEKLTFQDDSEPSENKYGITAGQLLRFVNNIKRNDIVVVPSE
                     GSERFLVGKVTGPLYELNQSQLEEYKSEELTHNRSDFAKRWKVYWLGWFNRSDADSAL
                     YKMIYSHATLSNINDYKPFINRALFPCYIEDEKLYISYHVTEEKDIQGVYLGQFVYQY
                     SLLTRLLFPETRVDSKINVQSEGIIELITHTVNYGLIISAILSGAIVLTSGGKLKFMG
                     LELEVPGLINTYQEYQKNKLERIKQAKELADELGVPISELGIRIPRKLTTAIESQKNV
                     KLASSNPPEKSGE"
     gene            1415..2542
                     /locus_tag="VYN16_001480"
     CDS             1415..2542
                     /locus_tag="VYN16_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1403992.1"
                     /translation="MWMEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMKEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESG
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(2630..3541)
                     /gene="whiA"
                     /locus_tag="VYN16_001481"
     CDS             complement(2630..3541)
                     /gene="whiA"
                     /locus_tag="VYN16_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1403993.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(3538..4515)
                     /locus_tag="VYN16_001482"
     CDS             complement(3538..4515)
                     /locus_tag="VYN16_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1403994.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(4512..5402)
                     /gene="rapZ"
                     /locus_tag="VYN16_001483"
     CDS             complement(4512..5402)
                     /gene="rapZ"
                     /locus_tag="VYN16_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1403995.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(5454..5834)
                     /locus_tag="VYN16_001484"
     CDS             complement(5454..5834)
                     /locus_tag="VYN16_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1403996.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(5845..6432)
                     /locus_tag="VYN16_001485"
     CDS             complement(5845..6432)
                     /locus_tag="VYN16_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1403997.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(6441..7673)
                     /gene="clpX"
                     /locus_tag="VYN16_001486"
     CDS             complement(6441..7673)
                     /gene="clpX"
                     /locus_tag="VYN16_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1403998.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(7705..7875)
                     /locus_tag="VYN16_001487"
     CDS             complement(7705..7875)
                     /locus_tag="VYN16_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1403999.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(7875..8381)
                     /locus_tag="VYN16_001488"
     CDS             complement(7875..8381)
                     /locus_tag="VYN16_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1404000.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(8511..9029)
                     /locus_tag="VYN16_001489"
     CDS             complement(8511..9029)
                     /locus_tag="VYN16_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1404001.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
BASE COUNT         2910 a         2011 c         1746 g         2714 t
ORIGIN      
        1 tataaaaaaa gaaatccctg ttttggattc ttctaccgac tattggctag tacgtgctaa
       61 ctctggagaa tattacacgg attttaatct aaatggctat ataggtatcg gatggaatga
      121 aatcaccctc gaagatatta gacgagcaga taataactcg aatgtattaa aagaaatttt
      181 aaaagagaaa ttaacatttc aagatgactc agaaccatct gaaaacaaat atggtatcac
      241 cgcaggtcaa ctccttcgtt ttgtaaataa cattaaaaga aatgatatag ttgttgtgcc
      301 atccgaaggg tctgaaagat ttttagttgg taaagtaacc ggaccgctct atgaacttaa
      361 tcaatctcaa cttgaggaat ataaaagcga agagttgaca cataatcggt cagattttgc
      421 aaaaaggtgg aaagtctatt ggttgggatg gttcaatcgt tccgacgcag acagcgcatt
      481 gtataaaatg atctattctc atgcaacact atcaaatata aatgattata aaccgttcat
      541 taaccgcgca ctttttcctt gctatattga agacgaaaag ctatatatta gctaccatgt
      601 aacagaagaa aaagatattc aaggggtata tttaggtcaa tttgtttatc aatattctct
      661 attaacaagg ttgcttttcc ctgaaacacg agtagattca aaaataaacg tacaatctga
      721 gggtataata gaacttatta cacacactgt aaattacgga cttataattt cagcgatact
      781 tagtggagca attgttctga caagtggggg aaaattaaag tttatgggat tagaactaga
      841 agttccagga ttaataaata catatcaaga gtatcaaaaa aataaactcg aacggattaa
      901 gcaagcaaaa gaactagctg acgaactcgg tgtaccaata tctgaactag gcattcgtat
      961 tccaagaaaa ctaacaacag ctattgaaag ccaaaagaat gtaaaacttg cttccagtaa
     1021 tccgccagag aaatctggag aataaaaaaa gtctaacatc agttagactc gaagaaaatg
     1081 aatttcttta gttttaatga gataagcata gctatcgcta aggaaagata atcgtttgtg
     1141 ataaaaattg tggttattga gaaaaatatc ttgaaaatca agagaaataa aaatatagat
     1201 acaataaaat tttttaactt tgttttcaac atattttttc tccttattct tttgaaacaa
     1261 ttataacaca attatctgtt aaagttaaat aattctaaaa aatccccaca ctctccatcg
     1321 ccaaactttg agtgtgagga tatcctggat agtaaaaggc attaaaaagc cctctttact
     1381 atacccattt tatcaaaaag tgaggttaaa atcaatgtgg atggaagaat taccaaacgg
     1441 caaatataaa ttttttgagc gatacaaaga tccatatact gagaaattaa aaaaagtttc
     1501 agtaaccatg gagaagaaaa ctccccaggc aagaaatcaa gctgctatct tgttgcaaga
     1561 gaagataaat aaaaaactta gcacaaaaca agtagaaagc attacatttg aagaaatcta
     1621 taaccttttt tataaatcat gggcgcaaac agtaaaggaa tcaacaaaac ataattgtaa
     1681 atcagttgat aagaagatga aggaagtcat accatccgat accatacttg ctaatcttga
     1741 caggcgtttt cttcaagagg ctattgaaaa aattattgaa agcaacggat atattacagc
     1801 taaaaaagta cggcataggc tcagaggtat ctttaattac gctgttcaat actcttacat
     1861 tgaaaacaac gaggtcgatt atactacgat tcctcaaaaa ccaaagactt tagaagaact
     1921 ggaaaaaaag cgtaacaact ttctcaccat gaaagaaata aaagcacttg tcgatgtcct
     1981 taatcgtcga gaatatcacc aaaagtacgc tgatatggtt cttgtgctga cattaactgg
     2041 tatgagatat ggtgagttaa ctgccttaca actgaagaat atagacttcg aaaacaacaa
     2101 aattgagatc acaggtaatt ttgattcagt aaacaaaatc aagacgctac caaagactac
     2161 aaattcaata cggacaatca aagtatcaga gagtgtcata gaagctattc aaagacaaat
     2221 agtacgactt agcgaacgtt tccagccatt gtcaagcgat gattatattt tctgttttga
     2281 aaaatggaat caacctacaa caatagcttg cttcatacag atattaaaaa aatatggaaa
     2341 acaggccaaa atagaaaaaa acttatctag ccatattttt aggcattctc atatttcgtt
     2401 tttagcagag tctggcctcc caataaaatc aataatggat cgagttgggc actcaaatgc
     2461 aaaaatgact ttggaaatct attctcatac tactgaggat atggaggata aactggtcaa
     2521 taaattagat actatttttt aattctgccc cttggttgcc cctttttgat ttcataacac
     2581 aacaaaaccc cttgaagata ttgataattc aaggggttgc tttatatcgt tataattcat
     2641 ccgctatctt attgattttt ctgagtctgt gattgacacc acttttggtc agaggggtgc
     2701 tgagactatc tgctaactgc tggatagagt agtctgggtg ctgaatcctc agttgcgcca
     2761 cttcctgcaa atctactggc aaattttcta agcccatgat atctttgatt ttactgatat
     2821 tgttaatggt cttcatgctg gcagaaactg tccgagcgat attagctgtc tcggcattat
     2881 tagcccgatt gaggtcgtta cgggtttctc gcaaaatctt aacccgctca aaatcatcac
     2941 gtgcctgcat ggctccgatt actatcaaga agtccataat gtcttctgct cgctggagat
     3001 aggtcacagc ccccttcttg cgctcaagca ccttggcatc cagtaaaaac tgttggagaa
     3061 gggaggcaat tccttgcgcg tggtccagat aaacagaact gatttccaac tggtacttgc
     3121 ctgactcagg gtcacgaatg ctcccatttg ccaagaaagc gccacagaga taggcacgac
     3181 ctgcttcctc atccgataaa atcgcctcat caatacctgt ttccaggcca aagaaagagt
     3241 ctgccaagtg caaatcactt aacaaatcct gcaccttttc atctgtaaaa acggtataga
     3301 cgcgattctt gcgaagattg ctccgttggt ggtgacgaat ttcagatttg atttcataga
     3361 aatgaagaaa ggactcatag aggtgacggg ccagtttggc attttctgtc acaactgaca
     3421 aagtcaagcc cgaagtcgag agaccgatgc taccagacat tttgataatg gcagataatt
     3481 catgccagct cagatggtgt tggcccagga tttcttcttt tactgctact gtgaaactca
     3541 ttttttcacc tgtataatgc gcatcaactc gtccacaatc aaatctccat cgtggaaggc
     3601 accgccattt tccagacgaa ggaagttaga tgaaatcacg cgcgaaactt gcttacaaag
     3661 acctacaaaa tcgtgttcca cttgcactaa gtattcatca aaacggttgg aattcatgta
     3721 ttcctgaggc actttttcaa tattcaccaa gacagtgtcg ataaaagggc gaccaaggtg
     3781 acgatgcaag acttccacgt ggtcgctatc tgtaaagtgt tccgtctccc cacgttgggt
     3841 catgatattg cagacatagg caatttctgc cttagtttcc aaaagagccc gcccaatttc
     3901 cttaatcacg atattgggca aaatagaggt aaagagggaa cctggcccta ggacaatcat
     3961 gtcactttca aggatggtct gcactactcg acggctggcc agaggcgtat catcgtttag
     4021 ggcattggtc acatagacat tgtcaattat gcctcgatgg tctacaatat gactctctcc
     4081 agccacttct gtcccatcct gaaagactgc atgaagggtc aaaggatggt cactggaagg
     4141 ataaattttc cctgttgtat ggaaaaattt gctcaataac tgcatggcat tataggttga
     4201 accctgcatt tctgacaagc cagcaatgat gagatttccc aatggatggc cagcaaaggc
     4261 tccggcatcc tcagagaacc gatactgaaa gaccttctca taaaacttag gcatatccga
     4321 catggccaca aggacattac gaagatcacc tggcggtgtc aactgttgca tattttttcg
     4381 gagttcacct gaagaaccac catcatctgc caccgtcacg atagctgcga tttccacatc
     4441 tttttcccgc agacttttta gaatgacggg aattccagtc cctccaccaa tcaccgttat
     4501 ctttggtttt ctcatgaacg gtttaccgtt tcctttctgc ggtctttgtc gcgatgccct
     4561 tcattaacag accaattctt ggataagtcc tgcgccaagc gtttagcaaa tgccacacta
     4621 cggtgttgtc cacccgtaca tcccatagca atggtcaaaa cggacttacc ttccttttgg
     4681 taacttggca gaatcggctc aatcaaggcc aataaatgtt gataaaagtc ttctgactca
     4741 ggatggttca tgacataatc ataaacaggt tcatccacac ccgtttggtt tctcagttct
     4801 ggtagataat agggatttgg caagaaacgg acatcaaaga ccaagtccgc atcaatcggg
     4861 attccatact taaatccgaa agacatgact tcgatacgga aagactgggc ttgttcttgg
     4921 tctgaaaact gctctgcaag ggttttgcgc agttcacgtg gagtgagttc agtcgtatcc
     4981 accacatttt ggctcatatt tttcaaaggt gccaagagtt cacgttccaa cttgattcca
     5041 tctaaaatac gaccgtctgc tgctagtggg tgactccgtc tggtttcctt gtaacgagcg
     5101 accaattcct tatcagccgc atccaaaaag aggattttga aatccaaacc atcttgattt
     5161 tccaactcat ccaaaacagc ttgaatctct gaaaagaaag aacggctacg catatccact
     5221 accaaagcca acttaggatt atcttcctta atttcaacca gctgcaaaaa cttaggcaag
     5281 agagctggcg gcatattatc aatggtgaaa taacctagat cctcgaagga ctgaatggct
     5341 acagttttcc ctgcgccact catccctgtc acaatcacca agtgaagttg tttctttgtc
     5401 atctttttct ccttatatca aaagaagttt ggcaacacca aacttcaact agcttatcca
     5461 atctctgcga tgacttcaat ttcgactttt acatcacgag gaagacgagc tacctccaca
     5521 gctgaacgag ctgggaattc ctctttgaag gccgtttggt aaacctcatt aaaaggaaca
     5581 aagtcgttca tatcgctcaa gaagcaagtt gttttgacaa catggtcaaa gtctgttcct
     5641 gcttctgcca aaatagcacc gatgtttttc aagacttgct ctgtctgttc ttggatattc
     5701 tctcctacaa tttccccagt ttcaggggat aggggaactt gaccgctagc aaacaaaagg
     5761 ttgccaacga tttttccttg aacatagggt ccgatagcct ttggggcctt atctgtatga
     5821 attgtttttg ccattttctt ttcctcacaa tttttctaag attgcatccc aagcctcatc
     5881 catccctgcc ttgctgacag atgaaaagag gatgaaatcg tcactcgggt caaagtttaa
     5941 tttctttttg attgctgatt catgcttgtt ccatttacca cgaggaatct tgtccgcctt
     6001 ggtcgccaca atgatgactg gaatctcata atacttgaga aattcgtaca tctgcacatc
     6061 atctgctgac gggtcatgac gaaggtcaac tagactgaca accgcacgga gattttcccg
     6121 agtcgttaag tactcctcaa tcatgcaccc ccacttttca cgttcctttt tagaaacacg
     6181 agcatagcca taaccaggca catccacaaa gcgcatcttg tcatcaatgt taaaaaagtt
     6241 caggagctgg gttttaccag gttttcctga tgtacgggcg agattcttac ggttcaacat
     6301 agtgttgata aagctggatt taccaacatt tgaacgccct gctagggcaa tctctggcag
     6361 ttcatcctgc ggatagtggg acttattagc tgcactgagc aagatttcag cattgtgtgt
     6421 attaagttcc atagtcacct ctaggctgtt tctaggatcg gtttatccgt tccatcgaca
     6481 gtttctttag tgatgcgaac caatttcaca ttttcctgac tcggcacctc aaacataaca
     6541 tctagcatgg tttcttcgat gatggagcga agtccacgcg cccctgtctt ccgttcgatt
     6601 gctttattag caatctcttg aagggcttcg tcgtcaaatt ccaactcaac atcatcataa
     6661 gaaagcaagg tttggtattg tttcaccaag gcatttcttg gctctttcaa gatgcgaacc
     6721 aagtcatcaa cggtcaattg ctcaagagcc gcaaaaacag gcaagcgtcc aatcaactca
     6781 gggataatac caaatttttg aatgtcttca gcgatgattt cttgcatgta tgagctgttt
     6841 tcgtcaatcg ccttattgtt ttgaccaaat ccgatgactt tttcacccag acgttgtttg
     6901 acaatttctt caataccatc aaaagcacca cccacgatga agaggatatt ttttgtatcc
     6961 acttgaatca tctcttgttg tggatgtttg cgtccacctt gaggcggtac gctagcaaca
     7021 gttccctcga taatcttgag aagggcttgt tgcacccctt caccagaaac atcacgtgtg
     7081 atagacacat tctcactctt cttggcaatc ttgtcaattt catccacata gataatgcca
     7141 cgctctgcac gttcgatgtt aaagtcagca acctgcaaga gtttgaggag gatattttcc
     7201 acatcctcac ccacataacc agcctccgtc agagctgtcg catccgcaat agcaaaaggt
     7261 acattcaagc tcttagccaa ggtctgggca aggaaagttt tccctgaacc agttgggcca
     7321 atcatcaaaa tgtttgactt ctgcaaatcc acatcttctg actcttcgcg tgtatcgtgg
     7381 aaattgatgc gtttgtagtg gttataaacc gccactgcca aggcacgctt ggcacgatct
     7441 tgaccaatta catagtggtt caagatatgg aggagttcaa ttggttttgg cacctcagac
     7501 aagtctgcca agacttcctc aaccaattct tctcgaatga tttcctgagc taactccacg
     7561 cattcattac aaataaaagc attgttgcca gcaattattt tttgtacttc ttcttggttt
     7621 ttgccacaaa atgagcaata aaccatcata tcattttttc tatttgtaga catgatttcc
     7681 ttccattcta tactgtcatt ctatctaaaa taaggtcatg taaaaagcat gaatactatt
     7741 gaccagattg gtaaaggcat ttaaccaaag gaggatagaa agcccgtaac gctttttacg
     7801 aaaagcttgt gctcctgcca gaaagcagat gaaacacaga aaagccgtga atagaccaaa
     7861 taaactccgt tccattagac ttcctttctc ttgcggtatt ggatggtaaa atcataagga
     7921 ttcttctcat ctttggcgta aaatttgctt gaaactgtct caaaaagaga caagtcaagt
     7981 tcttcaggga aataggtatc tccttccacc cgagcatgaa tgtgagtgac aatcacttca
     8041 tcaaggtaag gttcaaaagc ctgaaaaatt tgcttcccac cgataatgta gagattcttt
     8101 tcttgagcct gataccagtc aagaacagac tggacgtcct gaaaagtagc aaccccatct
     8161 atcttttctt ccggattacg cgtcaaaatc aaggtttccc gttttggaag caagcgacgc
     8221 cccatcccat caaaggtcac acgccccatc aagatagcat gattcagagt tgtttcttta
     8281 aagtgctgca attctgctgg caaatgccaa ggcagacgat tttccttacc aatcacaccc
     8341 tcttcatcct gggcccaaat agctacgatt ttcttagtca tgcttccatc cttttcactg
     8401 atagtactat tttatcaaaa aactcaaaaa aagactggtt tggaatagct tacaaaatag
     8461 aaaaaatctg taagaaattt cctacagatt tatctatgtt tccttatttc ttacaaacca
     8521 ggtgcttgtc caagttcggc tgcaagcatc caaattgttt tatctgtttc agttttagcg
     8581 cctgcaaaga taccgtttgt cacatcgtca ccttcttcat cagtgacatc caaacctttt
     8641 tggaaaagtt ctgacaagta acggtagata acaagaacac gttccaagct ttcttcaaca
     8701 ttacggtatt caccagcttc ttcttcgatt tcactatttt gaaggaactc tgtcaatgta
     8761 gagaatgggc ttccaccgag tgtaatcaag cgttcgctga tttcatccaa ttgaccgtca
     8821 agagcttcca tgtactcatc catttttgga tgccatacaa ggaaaccacg accatgcata
     8881 taccagtgca cttggtgcaa agcaacgtga gctacataca aatcagcaac agcttggttc
     8941 aagacttcct ttgtttttgc caatgctact ggcgctgctt ttgtcaatga tgttacgtct
     9001 tttactgctt cttttttcaa ttctaccatt ttctttacct cattcattat tttttgtaac
     9061 cacttcttaa tgattactac cttagtatac tactaaggaa aaccaatagc aagacaaaga
     9121 acttatatga gaaaagttgc aatagactga taatttaaga attctttaga agacaaaaaa
     9181 gagggttgga tgcttatagt caatggaaat ccatcagcaa actaggaaac taaacccagc
     9241 ttgaccaaag cacaagtttt gaggtcgcag gtagaactga ccaagctgat gtggtttgaa
     9301 gaaattcccc aatactataa tagattgaaa taagatgaaa ccaagtcgat taggaaaggt
     9361 aaatgaattt ctaaaatgtt t
//
LOCUS       DAVLTM010000073         9286 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLTM010000073 DAVLTM010000000
VERSION     DAVLTM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.074"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            239..1048
                     /locus_tag="VYN16_001490"
     CDS             239..1048
                     /locus_tag="VYN16_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1404002.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(1228..2103)
                     /gene="aguB"
                     /locus_tag="VYN16_001491"
     CDS             complement(1228..2103)
                     /gene="aguB"
                     /locus_tag="VYN16_001491"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1404003.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(2113..3198)
                     /gene="aguA"
                     /locus_tag="VYN16_001492"
     CDS             complement(2113..3198)
                     /gene="aguA"
                     /locus_tag="VYN16_001492"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1404004.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKDAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(3195..4322)
                     /gene="nspC"
                     /locus_tag="VYN16_001493"
     CDS             complement(3195..4322)
                     /gene="nspC"
                     /locus_tag="VYN16_001493"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1404005.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(4322..5581)
                     /locus_tag="VYN16_001494"
     CDS             complement(4322..5581)
                     /locus_tag="VYN16_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1404006.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(5578..6438)
                     /gene="speE"
                     /locus_tag="VYN16_001495"
     CDS             complement(5578..6438)
                     /gene="speE"
                     /locus_tag="VYN16_001495"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1404007.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            6694..7409
                     /locus_tag="VYN16_001496"
                     /pseudo
     CDS             6694..7409
                     /locus_tag="VYN16_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7375..8850)
                     /locus_tag="VYN16_001497"
     CDS             complement(7375..8850)
                     /locus_tag="VYN16_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1404008.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         2688 a         2090 c         1787 g         2721 t
ORIGIN      
        1 gagtaatctg atactcatac ctacttacaa aaaaactatt atattaagtt ggttttttaa
       61 tttgtctagt tgcaacattg acaaacatag tatagcatat ctttgccaaa tatcctcttc
      121 aaatcatgaa ttgtcatcaa aacatcttaa actataaaat caattagtct caagctttct
      181 atcaatttct tctccaaaat atgctataat aataccaaaa gataaagaag gaagacctat
      241 gattaaacta ctagccttgg atatggatgg aaccctcctc aatgaagcca aggaaatccc
      301 acaggctcac atcgctgcta ttcaccaagc cattgaaaaa ggtgtcaaac tggtcctctg
      361 tacgggtcgc ccccttttcg gtgtcctccc ctactacaaa aaactggggc ttgacctcca
      421 gaacgaatat gtgattgtta acaacggttg ttcaactcac cagactagcg actggggctt
      481 ggttgactgg caagaactga gtccagctga catcgaatac ctctatgacc ttgctgaaaa
      541 gagtgatgtt cagttgacac tttttgacga gtcacattat tttgttctcg gtggcaagcc
      601 caatcaagtt attgaaaatg atgctaaact agtattttca gacctgactg aaatttctct
      661 tgaagaagcg actagtggaa agttccggat gttccaaggt atgtttttag gaacaaaaga
      721 acaaacagac gattttgagc agcgttttgc tgaagagctt tgccaacgat tcagtggagt
      781 tcgttcgcag cctgtcattt atgaagcaat gccacttgga acgacaaagg ctactgctct
      841 ttcacgactt gctgagattt tgaagattga ttcctcagag attatggcca tgggcgatgc
      901 taataacgat atcgaaatgc tccagtttgc agggcttggg attgcaatgg gaaatgccag
      961 cgattatgtc aaatctcttg cggatgccgt tacctcaagc aacgaagaag acggcgttgc
     1021 gcgtgctatt gagaaatata ttctataaaa aagaaaagca aatagacaga agttactgct
     1081 atttgctttt ttgctaatat ttaaaatacg gactaatcaa tagacaagaa tagcgaatca
     1141 taaaaatcga tttactagat gccatagtat cttatagttg ctaataagaa gttagtctag
     1201 cagaatgaat ctctcatttc tcccacacta atctgtaatt tgtctataca tttctggtct
     1261 tctatctcga aacaagcccc agtttagacg ttcacttgct cccttgtcta ggtcataagt
     1321 agctaacaga acagcttctt cttgtctttc agctcgttct agaatagctc ctgtttcatc
     1381 cgtcataaag gaggaaccgt agaagtcaag actggaactc tgtccgccat tttcctcact
     1441 aggagtaacc tcctctaaac cataacgatt ggctgcgatg actggaacaa tattcgctgc
     1501 tgcgtgccct tgcatagtac gttgccagtg accacaacta tctgtatcca aaattggctc
     1561 tgaaccgata gctgtaggat aaaagagcaa ttcagcacca ttcaatgcaa gacagcgcgc
     1621 tgtttcaggg aaccattgat cccaacagat accgatacca atcttagcat agcgagtatt
     1681 ccagaccttg aaaccagtgt taccaggcgt gaaatagaat ttttcttgat aataatggtc
     1741 atctggtata tgggtctttc gataaacgcc cagcacttcc ccatctgcat caatgacggc
     1801 aatagagtta tacaagacat taccatcttt ttcatagaaa ctgattggta aaacaacttg
     1861 tagttcctta gcaatcacct taaaatgctg aatggcagta ttttccgcta cagattgggc
     1921 atactggtag tagtcatact gacgttcctg acagaaatag ggatgttcaa acaactcggg
     1981 caagagaata atttgggctc cttgctcagc agcctgacgt actaaacgct ctgcggtttg
     2041 gatatttgtt gccacatcct tagcgcattg catctgaatg gttgcaactc ttacatttct
     2101 catctttttc tcctattctg gaatttgttg ggtgatacag tggatattgc caccacctaa
     2161 gagaatatct ctggctggta ttccgacaac tttacggtct gggaaacact tgctgaggat
     2221 atctaaggcc acttggtcgt ttacatcctc aaactgtgga accaagacag ccttgttggc
     2281 gatataaaag tttacgtagg aagctgctag tcgttcacct gcgtatcgct cttcttctcc
     2341 ttcttcatag atgtagcctg gcaaatcttc ttctgtcaca acttgtcgaa ctgcagggat
     2401 aggcaattta tgaatggtga agtgacaacc ttttgcatct gtttcctgtt ctaagagttc
     2461 gagatctgct tttgacatgg catactgggg atcattttcg tcatctgtcc aagccaaaac
     2521 aacctcagca ggaccaacaa aggcagcaac attatcgacg tgttcattgg tttcatcctg
     2581 ataaatacca taaggaagcc aaataacttt ttcagcacca agactttcta ataatgtgtt
     2641 ttcaatctcc tctttagtca agttaggatt gcgaccagga ctaagcaagc aactttcagt
     2701 tacgagaata gttccttgac catcgctatg gattgcacct ccttccagta caaaaggttt
     2761 agcatcatag acaggccttt ccaaggcctc agcaaaacga ctggctactt ggtcatcctc
     2821 ttcataatct tgataaagac catcataggt gcctccccaa gcattgaagg cccaatccac
     2881 ggctaatttc ttacctttat cattgacgag aatggttggg ccagtatcac gcgcccaggc
     2941 atcattggtg ggaatgtcta aataaacaac cttgtcccca agataggatt gggcttcaga
     3001 tagataggcc tgctccacca aaagatagac tctttcccct tctgctatag tctcgatgat
     3061 ctgagtaaat gctcttttag catcctttcc ttgaaaaggc catgatcctg gtcgagtcgg
     3121 ccatatcatg agggtaccat gatggggttc gtactctgct ggcatgtgat agcctaattt
     3181 ttttggactg tccatcatga taatctccct ttaaagtctt gatagccaaa agctttgagt
     3241 aagctacagt ctccctgttc gtccatgaga tagagacttg gcaatccaat accattaaag
     3301 gtattatttt tgacaaaaga ataaatggcc atgtcttgaa aataaagtct gtctccgatt
     3361 tggactggat tttcaaaact ataatcacca atcacatcgc ccgtcagaca ggtattagaa
     3421 gaaagtctgt aggtatgggc tttttcctgt gcctcaaagc catttctcaa aggtggacga
     3481 tagggcatct caagtacatc aggcatatgg caggtcgcag aggcgtctaa aaccaagatt
     3541 tccataccgt tttctacaat atctaatacc tcagttgcta aataacccgc attaagcgca
     3601 atggcttcac caggctcgat atagatttca agattgtaag tttttcggat acgcttgatt
     3661 tctgaaatca gcaaatccac atcgtaacct tctcttgtaa tatgatgacc accacccata
     3721 ttgagccatt ttacctcatg taagtaggga ccaaactgtt cttctactgc tttcaaagtt
     3781 gtttgtaaat catctgctcc ctgctcgcaa agggtatgaa aatgaagtcc gtcaaccaaa
     3841 tctagcaaat cactcggaat cttgtctata gtaactccaa agcgagaacc tggtgcacaa
     3901 gggtcataga gcgcgtgatc tccttgagtt gaacactgag ggttgaggcg caaaccaaca
     3961 ctgacaccag cctctcgaca acgagcccca tgcttacgca actgtctctc tgagttaaag
     4021 acgatatggt ctgttatccc aagcaattcc tccaagtctg catccttgaa agcaggcgca
     4081 aatacatgga cttcaccagg aaattcttcc cttgccaatt tggcctcata gagtccacta
     4141 gctgtcgtac ctgatagata ctggctaatc aagggataag ttttgtagag ggaatatgcc
     4201 ttctgggcaa gcaagacctt gcaaccgacc tcttcttgta catattgtag aatgcggcaa
     4261 ttagcttcta acttggccaa gtcaataaca taggctggtg ttggtacttg ttctaacttc
     4321 attagtccac catttgtgga ttttcaacca caacccatgg caaaccatac tcattcaaag
     4381 cttccatgaa tggatctgga tctaactcct caaggttata cactccagct tgtttccaag
     4441 ttccgttcat gactaatttt gtcccaatca tggctggaac gcctgtcgtg tatgaaattg
     4501 cttgtgaccc gacttctgca tagcactctt gatggtcgca aacattgtag atgtagatag
     4561 tcttttcaac gccatctttg acacctgtaa agatacatcc aatattggtt ttacctacag
     4621 tacgtgggcc aaggctagca ggatctggaa gcaaggcttt caaaaattga attggaacaa
     4681 tttcttggcc gttaaagtta atggtatccg tacgaaggag tccaacattt tcaagacatt
     4741 tcatgtgcgt caagtaagat tgaccaaaag tcataaagaa acgaatgcgt ttgacacctg
     4801 gaatgttctt ggccaatgat tcgatttctt catggtgaag gagatacata tctttttgtc
     4861 caacttgagg gaaatcatac tcacgcttga tagacatagc ttcgacttcg acccatttcc
     4921 catcttccca gtaagaacct ggcgcagaaa cctcacggag attaatttct ggattaaagt
     4981 tggttgcaaa tggataaccg tggtcaccgc cattacagtc taaaatgtcg atataatgga
     5041 tttcatcaaa atagtgtttg agggcataag ctgaaaagac actagttaca cctgggtcaa
     5101 agccagaacc aagaagagca gtcaagcctg cttctttgaa tttctcttga taagcccact
     5161 gccatgagta gtcaaagtag gctgtaaaac caagttcctt acaacgtttt tcgtagatag
     5221 cacgccactc agggtcttct gtgtcttctg cttcgtagtt ggctgtatcg atatagtgaa
     5281 cacctgttgc caaacaagca tccataatgg ttaaatcttg ataaggcaga gctacattca
     5341 aaacagcttc tggtttgtag ctttcaatca gggcaatcac ttcttcaacc ttgtcagcat
     5401 caagtgctgc agtttcaatt ttagtacttg ttttgccttc tagcttcgct ttcaagtcat
     5461 cgcattttga cttggtacgg ctagcaatca taatctctgt aaatgtttcg ctatcttgac
     5521 aaatctttga aatagcaact tgggcaacgc ccccacaacc aataactagt aaacgactca
     5581 tttttttcct tcctcttctt ctaaaatgtc ctcaacatac ttgggcaaca taaaggatcc
     5641 cacgtgtaag tttgcagtgt agtattctgt gaaaagctgg cgttttttcc aaccttcctt
     5701 gtcaaaatct ttgacagggt ggtatttttt cgatgcaaat ccaaacaacc aatagccagc
     5761 tgggctagtt ggaatatggg cctgataaac ccgactgatt ggaaaggatt gattgacctt
     5821 gcggtgcatg cttcggcagg ccgactcatc ctcgtcaaag aagggactcc catgctggta
     5881 aatcatgatg ccgtcttcct tcagagctcg ataactattg ccgtagaatt ccttggtaaa
     5941 gagtccttcc gtatggccaa atggatctgt cgcatcgttg atgataatat cgtaatcatc
     6001 ttcgcagttt cgcaaaaagc gtagcccatt ttggtagtaa atggtaacac gaggatcatc
     6061 tagccctgca gcaaagtctg ggaaatactc acgacagacc tcgaccaaca tctcatccgg
     6121 ttccacgata tcgatttgtt ccagttcagg atagagtgtt aatacttggg caacaccgcc
     6181 gtcaccaccc ccaataacca atactttctt tggatttggg tggacagcca tgggaacgtg
     6241 aacggtcatt tcattgtaga cgaaatcatc cgcatctgag aacaagacat ggccatttaa
     6301 aatcagtatt ttcccaaaag ctggcgtatc caagacttcg atatcctgcc attcactttt
     6361 tccagcgtaa agttgcttgg ctgttctcag agacaatttg acatctggag tatgaacttc
     6421 agaaaaccat aaatccatct atttctcctt atattatatt tagcgcagtt agctactgcc
     6481 aaagcccaag tggtatactt ggaataagcc actgtggatt agtttattcc ctccaatcgc
     6541 ttgacgattt ccgaaaggct ctaaccacag ttttttgttt caattggtaa tgagtttgat
     6601 aacgcataga cgttttatct cgtctaaggt aacaaggcaa aaaattcgtg gataaccaag
     6661 tagacttgaa atactacatc tggaggtact cccatgcttt atgtgggcat tgatgtcgct
     6721 aaaaataaac acgatgttac agccttgaat gttccaggaa aaactgttct taaaccactc
     6781 actttttcaa ataataaagc tggttttgaa ctcttagatc tgtctcttcg acaactcaac
     6841 caagactgtc tcatcgctct tgaaaatacc ggacattatg cctttaatct actgaacttc
     6901 ctgcatgaac aaggatataa agtttacacc tgcaatcctt tgctcatcaa ggaatttgcc
     6961 aaatcattat cacttcgcaa gactaaaacg gataagaaag acgctcatgg catcgctctt
     7021 aaacttcttt ctgaccccaa tcgtgaacaa tttcaacacg ataatcggca agtagaacta
     7081 aaaatactgg ctagacatat tcatcgtctc aagaaaaaca gtctgattgg aaagtacaat
     7141 acactcgttg tcttgatatc atctttcctg agttggataa aatcgttgga aagcattcag
     7201 aatataccta ccaactcttg acacgctacc ctaatcctca gaaaaggatt gaggcaggat
     7261 ttgataagct gatagaaatt aagcgattga ccgcttctaa ttcaagggct ttttgccgtg
     7321 ctctaaaatc atcgccaaat catcaaattg aaataagaaa tagtttagat aaaactattg
     7381 acttttctta tagtttgtct ttctcttaat aacgttgata tgattgacct ctggatcttc
     7441 cgtcccttgg agggagcaac cacgttcctt ggcgaattgg atatagtcta caatttctcg
     7501 tgtaatgcgt tcaccaggag ccaagatagg aatccctgga ggatagcaca tgacaaattc
     7561 tccacagacc tgtccaacag actcatctaa ggtcaaactt tttctctctg aatagaaggc
     7621 ttcttgtgga gacagcacca actcgggctg aatatattct ccagctatta agtccttccc
     7681 atctcgtgaa tagagtctct taatatcagc cagagcacca accaagcgct cgatgtcttg
     7741 gatgcggtcg ccgatggaaa tataggccaa gatattgccg atatcaccaa actcgatctg
     7801 aatgtcgtat tcgtctcgca agaggtcata aacctcgata cctgttaagc ctattccctg
     7861 agtgtaaact gacaacttgg ttacatcaaa atcacagacc gacacaccat ctattaactc
     7921 ttttgagtaa gcatagtaac cgccaatggc attgatttca tgacgagcgt actcggatag
     7981 ctcaatgact tcctcaaacg actctttacc acgaagggcc aagttgcgac gtgaaatatc
     8041 caaactagcc atcaataagt aagaggcaga tgtagactgg gtcaggttta taatctgacg
     8101 aacgtattcg ggattcatct gctccccgat taaaagaagc gaactttgtg tcaaactccc
     8161 accagactta tgcatggaaa ctgctgccat atcagctcct gcatccatag cagaaattgg
     8221 aagtttacca gtgaaatgca aatgcgctcc atgggcttca tctactaaaa ccatcatgcc
     8281 agcttcatga gccatttctg tcaacccctt taggtctgaa cagatgccgt agtaagtagg
     8341 attgttgatt aggatagcct tagcatctgg atggtccttt atggcctgtg ctactcggtc
     8401 attttcaaga cctaaagcga taccaatctt aggatctaca ctcatctcga tatagatggg
     8461 aatggcacca catagaacca acgcattgat agcagattta tggacatttc gtggcagaat
     8521 aatcttatct cctgccttgc aggttgccag aatcatagtc tgcaccgatg aagttgttcc
     8581 accaatcatt agaaaggcat ggctagctcc aaaagcatct gcagccagct cctctgcatc
     8641 acgaataatc gaaataggat ggcctaaatt atccaagggt ttcatcgaat tgacatcaat
     8701 gcctacacat ttttctccta agagttcgac aagttctgga tttccccgtc cacgcttgtg
     8761 acctggaaca tcaaagggaa caatcctttt cttgcgtaac ttcaccaagg cctcataaat
     8821 tggggcttgg ttttgatcta actctttcaa aacatactcc tttcaatatt tttaggacct
     8881 tatgaaaact aaggaactaa aggttgactc atgaaaaaag agcaggtttt cacctgctct
     8941 gcaatcttct gacgtgttta ttttggtttc tgttgagtat gtctgttccc gatgtttcct
     9001 aactctctag tagcaaatat tacgtacttt attgatcgtt tcacaagatg acgtgtgctt
     9061 tcaccacact aaaaatttat gaattaggac aagtgtccta acatcaactt ataaaagtag
     9121 gcttttaacc ctatcaataa tgtggctttt caaccacgct tcggcattca accctgcctg
     9181 tccgtaggca ttttttactc gggtttatat ttgctagaaa acatcatctc attttctaag
     9241 ctttattact atatcatgtt tttaagaact tgtaaagtat tttttg
//
LOCUS       DAVLTM010000074         9128 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLTM010000074 DAVLTM010000000
VERSION     DAVLTM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.075"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            37..512
                     /locus_tag="VYN16_001498"
                     /pseudo
     CDS             37..512
                     /locus_tag="VYN16_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            580..1032
                     /locus_tag="VYN16_001499"
     CDS             580..1032
                     /locus_tag="VYN16_001499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404009.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCEGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            1043..2275
                     /locus_tag="VYN16_001500"
     CDS             1043..2275
                     /locus_tag="VYN16_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1404010.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLAMLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(2393..3412)
                     /gene="cbpC"
                     /locus_tag="VYN16_001501"
     CDS             complement(2393..3412)
                     /gene="cbpC"
                     /locus_tag="VYN16_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1404011.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPTTGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNASNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(3510..4199)
                     /locus_tag="VYN16_001502"
     CDS             complement(3510..4199)
                     /locus_tag="VYN16_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404012.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            complement(4211..5635)
                     /gene="gndA"
                     /locus_tag="VYN16_001503"
     CDS             complement(4211..5635)
                     /gene="gndA"
                     /locus_tag="VYN16_001503"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1404013.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(5711..7081)
                     /gene="mapZ"
                     /locus_tag="VYN16_001504"
     CDS             complement(5711..7081)
                     /gene="mapZ"
                     /locus_tag="VYN16_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1404014.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSEGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            complement(7094..8251)
                     /locus_tag="VYN16_001505"
     CDS             complement(7094..8251)
                     /locus_tag="VYN16_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1404015.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(8336..8716)
                     /gene="rnpB"
                     /locus_tag="VYN16_001506"
     ncRNA           complement(8336..8716)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYN16_001506"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(8737..9066)
                     /gene="gpsB"
                     /locus_tag="VYN16_001507"
     CDS             complement(8737..9066)
                     /gene="gpsB"
                     /locus_tag="VYN16_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1404016.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
BASE COUNT         2589 a         2082 c         1604 g         2853 t
ORIGIN      
        1 tttttactag ataaaactgt aaattctgaa ggaaggatca cttcttcaac agaatttgga
       61 aatttcgtaa gtaatttatc attccaacac ggaatagctg gactactgtt tcctctaaat
      121 aaattgtacc ccccagaact ggattctaaa atactctcta tcatcaagaa ggcagtgaca
      181 attagaacga cacacacata taaatatcaa tactcactgc tatttggtga tgcaggctat
      241 ctatggttac tcctacattt attttctatc agtaaaaatc aatactatct acaattagca
      301 aacgtctccg ctaagaaatt aatagagaat tatgatactc tagaggaaat agactttgca
      361 ttgggaaaat ctggtgtcct attatcatta ataaaatact atcaatttac caatgacaat
      421 actcttaaaa ttttcatcca caatagtata ggggaaattt agcattattt cctacaaaga
      481 gatatagcca aagaaagcat tttagactat agctttgctc atggatattg tggaattgca
      541 taggctttat ttgcctattc taaagtctta gaaccttcta tgttctataa tgatctccat
      601 acattccata ctgaattaaa aaaattatta gaaaaagtta cttctaatac tgaaaattta
      661 ggaaatttac aactttcttg gtgcgaagga atttccggaa taatcttata tctttgtatg
      721 tacgattgtg acggaaacaa agatattatt agtaaatatc aagaatttgt ttttaaccat
      781 catctaaaaa tgatgacagg atattgccac ggaataacta gcttactaca aaccactgtc
      841 tacaatcaaa acaaattact gatgaaaaaa atccaacagg taattttagc atgttctgaa
      901 cgagatgatc acggtttact gatgtttcaa ggagatagtg gtaaagcaga tttgtttgac
      961 ttcggaatag gaagcatggg ggtatattgg tgtctattaa ataataaatt cccattcgat
     1021 gtgcagacat aaggagaaaa gtatgaaatt attttggaca aacaacatat atagacagtt
     1081 gctgctaaac agctgttttt catcattcgg cgacagtatt ttctacctcg ccattatcaa
     1141 ttatgtggct cagtacaatt tcgctccgct agcaatttta ctgatttcca tttcagagat
     1201 ggttccccta ctatcgcaac tctttctcgg gattctagga gattttcaag aaaatagagt
     1261 caaacacgca ctctggattg ccaaaatcaa aatcctgctc tacgctattt tgacagtatt
     1321 tctcgtcttg tcgccctttt cattagtttc agtcattatg attgtcatca tcaacctcat
     1381 ctctgacacc ttgagctacc tgtctgccta catgatgaac gccctctaca tcagtgtaat
     1441 taaggacgac ctgcatgatg ccatggggtt caggcagtct ctgatgaggg ttgtccgcat
     1501 tgtcgccaat ctggctggcg cattccttat caatgttatg agtattcaaa ctatttccct
     1561 tatcaacact ctgacttttg tcattgcctt tttgggcctg tatgttattc gacatacctt
     1621 gtatgaggtt gaaaaaagga ttgaaatgtc acatacagca ctgagtttta agaaatattt
     1681 tcaacatctt aaacagtcgc tggctgtgct cctgaggtta aaagataccg tcatactact
     1741 gtttctgacg accagtatga ttgccatctt ggatgtgtcc cctcggctga ttgccctccg
     1801 cttcatccaa cagacactag cacaactgag cattgggcaa ctcctcgcca tgctctccat
     1861 catcatttct tgtggagcta tccttggcaa tatgaccagc agtaatctat ttaaaaatat
     1921 ccgtttcacg cacctcttgg ttttctgtga gatttcccta ttggctctaa taactagtat
     1981 cctttgtcaa gcctatatcg taattttcat gaccagtttc atcagttcta cgattatcgg
     2041 cattctcagc cctcgcctac aagcagctgt ctttgcccat atccccagtg acaagatggg
     2101 gacggtgggc tctgctctga gcacagtaga cattctcgcc ccgtccctgc tctccctatt
     2161 agccctgtcc atagcatcgg gcgtttcggt gcagttagca ttgatatttt tgtatcttat
     2221 tttgattgct cttatctttt gtcaatggtt agtcaagttc aacactcata actaacgaaa
     2281 aagcatgtgt agatttcaca tgcttttaat ctccccaatc gtcaggtcaa gtacaacaaa
     2341 gtcacttctt tgattaagcg agtgttctaa tataattata agcgccctgt cattaccgaa
     2401 cccattcgcc attataattg acagaatagc catctacggt cgtattcact gccaaagcac
     2461 ctgagctata agcatagtac cagttgccat tgacctggaa ccaacctgtc ttcatatctc
     2521 cattacttgc atttaggtag taccaagttg aaccatcttg gtaccaacca gttgccatag
     2581 ctcctgatga acggagatag taccatttgt tcccaaggtt ttgccaacct gttttcatat
     2641 cgccatttgg ctggtctaaa taataccaag tggtaccttc ctgataccag ccagtggcca
     2701 ttgctcctga ggaacggagg tagtaccact tattacctag atattgccaa cctgtttgca
     2761 taataccagt tgttggatct aggtagtacc atggagcagg tttgcttcta tcaatgtcaa
     2821 tagtaacatg aaaatcttga gtccaaccct tagcaacttc accaattggt agtggattgt
     2881 aagaatcatc accaaaattt cctagcttat ttaaataata ccaattgccg tcttcataaa
     2941 tccagcctgt ctttacagca taattattat caaaataata agttctcttt tcagcagata
     3001 gaggatattg ttcaccgtat acttttcctg tattttcaga cgtttttgcc tctagtactt
     3061 gtttatctgt ttgttctagc aaagcaccat cttgtccaaa atagtaccac ttctcctgaa
     3121 ggccaatttc gaagactgga cgattatcaa ataaatcatc acgataacct gttccaggaa
     3181 tttctaagta ttgccaaccg acaaccatct ctccaatatc accaaaatag taagttttac
     3241 cgtctatttt atgccaatag attgctttat gatcatcttt tacataatat tttctattat
     3301 ccttatcaac aaatcggcca cctgtggtat tcgcaaatac cgtatttgta gctagcaaac
     3361 caaagaaaaa gactgctagt agaacttgca tcattttttt caaaagtttc atctatatat
     3421 cctcctaaaa cgattgtagc aaagacccga ctgcatgtca atatataaaa actcctcaaa
     3481 aaagcagtta cacaactgca actgcttttc tattcttgca tggtgtaacc aataccacgc
     3541 acggttttaa tgtagctttt ttgaccttta acatcaagct tgctccgtag atagcggata
     3601 tagacatcta cgatatttgt ctcggtcgca ctttcatact tccaaacact ttccaacagt
     3661 tgctcacgag tcaatacttt cttgcttccc ataagtgtcg ccaaaaggtc atactcacgg
     3721 cgggtcagag caatcatctc ttcaccacga taaaccgtgt gatgttcaac atctatccta
     3781 agattgcggt aggtccttgg aactttcatc agactgcagt gttgatcaat gaagtcccga
     3841 cctcggaaga tcgccgaaat acgcgctacc agattttcga taaagactgg cttatagatg
     3901 tatgaaactg caaaacgctg aacaacttcc agctcttctt gcaagtcttc ccaatgatct
     3961 aaaatcatga tgactgaggc aggtttagtt cgactcaatt tttctgcaaa atcctgagcc
     4021 atcatatctc ccagattaac gttcaataaa atcaaatcat agtctgtctg aagagccatg
     4081 gaaagggctt tttgcccctc ctctaccaga tcaacccgat actgctcttt ctggagttcc
     4141 aaacttaaaa aatgagctag atttcgttct ttctcaagta ataaaatccg tttccccatg
     4201 gctgacctac ttatttttcg tcataccaag agtagtggaa ggttccttct ttgtctttac
     4261 gttggtaagt gtgggcacca aagtagtcac gttgtgcttg gatcaagtta gctggaaggt
     4321 cagctgaacg gtagctatca aagtaagtaa tagctgctga gaaagttggc actggcacac
     4381 cagcttgaac cgcaagagct acgatatcac gcactgcttg ttgatactta gcagtaacat
     4441 ccaagaagta ttcatccaaa agaaggttgg caaggtctgc atcacggttg taagcatctg
     4501 taatcttttg caagaaacga gaacggatga tacagccatc acgccagata gatgcgatat
     4561 ctgcaaatgg caagttccag ttgttttctt tagaggctac acgcaattga gcaaatcctt
     4621 gtgcgtatga aatgattttt gagaagtaaa gggcttgacg gattttttca atcaattcag
     4681 ccttgtctcc ttcaaagttg aaggcggctg gttttggaag caccttgcta gcatgtacac
     4741 gttcttcttt gtaagttgaa atgtagcgtg caaacactga ctcagtaatc agtgacaatg
     4801 gtacaccaag gtcaagagat gattggctag tccatttacc agttcccttg ttacctgcag
     4861 catcaaggat gtagtctacg attggtccat cttggccttc atcgtcttta cggctcaaga
     4921 tatcagctgt gatttcaatc aagtagctat ccaattcacc cttgttccac tcagtaaaga
     4981 tttcagccat atcctctgca gaaaggccta gcaagtgttg catcaagtca tagctttctg
     5041 cgatcaattg catatcaccg tactcgatac cattgtgaac cattttcaca tagtgaccag
     5101 ctccatcagg accgatgtaa gtcacacatg gtttgccatc ttctggtgct ttagctgaga
     5161 tttcttcaag aacatccgca accaattcgt aggcttcttt ttgtccacca ggcatgatag
     5221 aaggaccttc aagggcacct ttttcaccac cagaaactcc agtaccgata aagttgatac
     5281 ctgagtttgc caattcttca ttacgacgga tggtatcttt gtagaaagtg tttcctccgt
     5341 caatcaagat atcacccttg tcaaggtgtg gaagaagggc ttggatagta gcatctgtac
     5401 caggtccagc ttgaaccatc agcatgatac gacgaggttt ttcgattgag tttacaaaac
     5461 tttcaacgtc atagcttggt acaaaattct tttcaggatg gcaagcaatc acatcttccg
     5521 ttttttcttt actacggttg tagatagcaa ctgtgtaacc acgtgattca atattaaggg
     5581 caaggttacg acccattacg gccataccta cgacaccaaa gttagctttt gtcatttgac
     5641 actcctcttg tttcatttgt tttattttat catttttact tttgaaaaga aagaattttg
     5701 taacgactgc ttagtagtcc aagtcatctg catgaccagc gccatttccg acaaagtagc
     5761 ctgtcttaca gttaagggta aagagatagg ttccatctgg cttatagagg ttgtaataac
     5821 cattgccgtt aacgatattg acacgttcaa ggatatattg gtctccagtg atatagccac
     5881 gtgaacgtga agtcgctaga atttgttcca agacaccatc cgcaaaatcc caggcagagt
     5941 tattactatc atcaatagca gactgattat aggcaacacg actggcactt ctttgaacag
     6001 caaccccttc actcgagaga cccatattga cttcactgcg actacttcta gtctcatttg
     6061 aactacttgg ttttggctca cttgtcgtat ttgagcttgc ttgacttgaa cttgagctag
     6121 aagtacttgt ttgctggctc ttaccaaggc taatagcctt atctagcact ttatcaagct
     6181 ccgtatttcc agttttaata tccgtaaatt tagcatccga tttggctttg gcattggtat
     6241 ccaacacacc atccacaata gctgattttt caaattgtgc attgacatct tgaatagcct
     6301 tgatttgcgt tgctagacta tcatatttag atttggcaag cgtatgttca cgactacctt
     6361 ccagtttatc aagcaaggtt ttgagttgac tcagtttatc aaactggcta tttttcaaag
     6421 ccgttttatt gctatctgtg taaaaggcgt cataaagtgt attaaaatca tccacatccg
     6481 attgattggc tgtagttgat tgagaagttt caatttcctt ggtcgaacga gccacttgac
     6541 ggtagacata ataagcactg acacaaataa ttactgatac aagcgccaaa acggtcaaaa
     6601 taaagccttt tttacttttc ttttctgagt cctcttgatc cagacgagaa aggttttgct
     6661 cctcttccac ttcttcagtc atttctgttg gattttcatc atctaacaat agaggatcca
     6721 agccctgctc ctcatcgtcc agaggcaagg gtagtaagat gtcttcagaa gatggaactt
     6781 cctccgaaga agcttctgac tcctctacag cctcacgcat ctcttgaatt aaatcatcca
     6841 gactctgagt ttcaactaac tcctcttttt tgtattgacg agtcgcaaac ttatccgcct
     6901 caatttcatc tctgtgttgc ttaacatact tgtccaaaat ggaatcctca ggcaagactc
     6961 ctgcttccac ttcttcattt ttacgaatag cttgaccaac tgttagctct tttgcttcat
     7021 caaaatcaaa ttgcggttct tgaccttctt ttttatgacg atttcgtctt tttttactca
     7081 tgagtatccc tttctatttt acctcttgcc gtttgacacg ctgaccaaaa tattgataca
     7141 gatccacttt caaggttccg ttgtataact tacgcttctt atctgcttgg ctaccatact
     7201 tgctttcaaa agcttcatca ctagtcagga taaatttgct ccaagttttc agcggtgcaa
     7261 atacttgccc catctcagca tagagcttgg tcacccctgc atcatctgac aaacgttcac
     7321 cataaggcgg attggaaatg attactccat tgattttatc ggaacgtaaa tcctgcacgc
     7381 gcatctgctt aaaagtaatg tctcctgcaa caccagctgc ctgagcattg gccttagcaa
     7441 tttccaccat gcgagcatca atatcacagc ccatgatatc cagctcaagc tcacggtcta
     7501 cttttttagc cgcttctgtg cgcacttctt gaatcaagcg atcgctgatc cagttccatt
     7561 cctcaaatgc aaaagagcga cgaagacctg gcgccatctt tctagcaatc ataactgcct
     7621 caatacagaa agtccccgaa ccacaggtcg gatcaatcaa aggcttgtct ggataccagt
     7681 tagaaagttg taaaatggct gctgccatat tttccttgat aggagcgcca cctttttcgg
     7741 tacgataacc acgtttaaag aggctagacc cggtcgtatc aatcatgaca gttgccacat
     7801 ctttgagaat agagacctca atcttaaact ctggaccatt ctccatcaga ggaacccctt
     7861 ctgggcgagc atagtgtttc tgcaatttct tgacaacagc tttcttagaa atagcctgaa
     7921 cactgggctc attgtgaagt ttggacttaa cacatttagc ttttgaaatc gggaaccgag
     7981 ctccaagtgg taaataattt tcccaatcta aagcgaaaac tccctgaaat agctcttcaa
     8041 aagtcttagc tgggaacgtt cctacgataa ttttgatacg atctgctgcc cgaagccaaa
     8101 ggttggtttc gataatagct ctcacgtctc cttgaaaacg aacacgtcca ttttcaacct
     8161 gacaatcgta gcccaactct cgcacttcac gaccaacgac agcctcaagc cctgctgcca
     8221 cagttgcaat taaattaaat tcttttttca tgttacagtc ttgcaagacc tttctatatg
     8281 tccactttaa aaaatgaggt tgagaaaatt tctcagcccc aacctatatg caattttcta
     8341 taagccatgt tttgttccag aagtgactag gaccacttcc ttcgataatc atctgtctac
     8401 cactaacagc tctagctgat agcagtcaga atactacgtt cgtttccgcg tactccatgc
     8461 cccgaccaaa atttgggttg ctagcttgag gggtttaccg cgttccactc tctctgtttc
     8521 cagaaagact ccgtcactgt ggcactttca agcctactct ggcctatcca aggacttagc
     8581 catttcaact gccgtaacga tttctcgtcc ctaggcttat ggtttcgcct agcacaaaca
     8641 ctacaggcat cacagcctgt gctagcatgg actttcctca tgagaagcaa ttctcctcac
     8701 gcgattatcc aaaaattgca catctcaaat aactacttaa aaatctgagt tatctaaaat
     8761 ttgtttacca aaaacttctt tttccaatct attcaggcgt ttcaaaatat caaaattcgt
     8821 catagaactt gtaattgccg cttcaagggg ttctgcttga actggtgaag gtttcggttt
     8881 acgagttaat tcttccttca aatccgcaat ttcctgacga agtgacttga ccaaggcagc
     8941 ataggtttca tagtccttga tgacatcgtc taaaaactcg tcaacttcta ctttattata
     9001 gccacggact tcacgtccaa actcttgttc aaaaatatct ttcgctgaaa aaataatact
     9061 tgccatgtct ctctccattc tcgcttgcta gtattattat ataaaaaaag gcaaacaaaa
     9121 atcaaggt
//
LOCUS       DAVLTM010000075         9080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLTM010000075 DAVLTM010000000
VERSION     DAVLTM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.076"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(54..1559)
                     /gene="lytC"
                     /locus_tag="VYN16_001508"
     CDS             complement(54..1559)
                     /gene="lytC"
                     /locus_tag="VYN16_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1404017.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(1597..2355)
                     /locus_tag="VYN16_001509"
     CDS             complement(1597..2355)
                     /locus_tag="VYN16_001509"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1404018.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(2453..3130)
                     /locus_tag="VYN16_001510"
     CDS             complement(2453..3130)
                     /locus_tag="VYN16_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1404019.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(3139..4083)
                     /locus_tag="VYN16_001511"
     CDS             complement(3139..4083)
                     /locus_tag="VYN16_001511"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1404020.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(4265..4777)
                     /locus_tag="VYN16_001512"
     CDS             complement(4265..4777)
                     /locus_tag="VYN16_001512"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1404021.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(4864..5622)
                     /locus_tag="VYN16_001513"
     CDS             complement(4864..5622)
                     /locus_tag="VYN16_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1404022.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAIDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            5830..5976
                     /locus_tag="VYN16_001514"
     CDS             5830..5976
                     /locus_tag="VYN16_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404023.1"
                     /translation="MEIKEKTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(6123..7253)
                     /gene="ugpC"
                     /locus_tag="VYN16_001515"
     CDS             complement(6123..7253)
                     /gene="ugpC"
                     /locus_tag="VYN16_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1404024.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(7537..7622)
                     /locus_tag="VYN16_001516"
     tRNA            complement(7537..7622)
                     /locus_tag="VYN16_001516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7586..7588),aa:Leu,seq:aag)
     gene            complement(7704..8279)
                     /locus_tag="VYN16_001517"
     CDS             complement(7704..8279)
                     /locus_tag="VYN16_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1404025.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(8279..9067)
                     /gene="codY"
                     /locus_tag="VYN16_001518"
     CDS             complement(8279..9067)
                     /gene="codY"
                     /locus_tag="VYN16_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1404026.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         2679 a         2041 c         1534 g         2826 t
ORIGIN      
        1 taaaacaaag aaaaagaggg tgctactatc acatccctct ttcaaatcat cgcttaatac
       61 caaacgctga catctacgcg cccttggatt cctttcatgt attcagaaga ggtatattgc
      121 caaccttttt ctcctgaata ataagggttt tcccattcta aagcattcgt ataggccgct
      181 acccagttta catgttttaa aatatctggg tgttttaaac gcgtctgtaa taaactacga
      241 tagctataga catacacatt ttgataaccc gcctgcttca tcgtgtccat gtacttgttg
      301 atgattttaa cccaagtgtc tgtatcactt ggagctctct tgctcttatt tacatattcc
      361 cagttctcaa catcataata gatagggtaa gacaggttca tattgtattt ctttataagt
      421 tcaatggtct gtttagcgtc attctcagca tcggtctcat tttcagcata ggtatagaga
      481 tagacaccat aaggaattcc cagacggttt aactccttaa tgttatgcgc caattccttg
      541 tcttctttac cgctataacc tagacgaaca atgacaccat ccacctcgtt ctcatcaata
      601 acctttttcc aatcattgat acgaccattg tgctcactaa tatcaatgat tttcttagca
      661 tgttcggttc ccacttgttc ttctctatta ttaaagaaat agcgtttacc atctctgtga
      721 acccagccga cattcaaatc tttcttttct ttgagctcac cagaagccgc aaagatatag
      781 cgagcaccat ccataatcac ttcgtcagtc gccatggcac cacttccagt caaatagtag
      841 ttgccttgcc actcattccg agccatatag ccccacttct tgaaatagta ccatttgcca
      901 cccacttttt gccactcttg gttagcataa gttccatcgg attttagata aaaataagca
      961 gaataagctg gatcatagag ccattcattt tgcatcatag ctccttgacc attcaagaaa
     1021 taacttcctt gccattggct tttagccatg taaccccact tcttgaagta gtaccactta
     1081 tttccaatca attgccattc ttgatgagca taagccccat ctgacttgag ataaaaccaa
     1141 ctcttgtaat tactgtcata gatccactcg ttttgggcca tatatccacc tgatttcagg
     1201 tagtaatttc catgccattc attctgcgag taacgaccat ctgaattaat ataaaaccaa
     1261 gccttatagt agttgtcaaa aatccattca ctttgagctt tagaaccatc atcctttaca
     1321 taataatccc catcccagtg ggcagcagca ttagtctgta cttctgcgct cgtttgcgtt
     1381 ttattagaag actgattttg ctctgaacta cttgaagctg tcgttgtatc ctgcgaagtt
     1441 tttgctactt cagtttcatt tgcagcgaca tgactagttg ccaagcctag taaacagata
     1501 cttgctaatc caatttttat tttcaatctt tcctctccta taaaaaatgt aacagacatc
     1561 tgaatgctgt tccacctagc ttttgctact tactgattat tttacaaagt caagcaaagc
     1621 caagaagctt tcagcttcaa gtgacgcacc acctacaagg gcaccgtcaa cgtctgggca
     1681 agccatgtat gaagcaacat tttcaggttt aacagaacca ccgtattgaa cacgaacttt
     1741 gtctgcgact tcttgaccaa agtcagcagc tacaacgtca cgaacaactt tacacatttt
     1801 ttgtgcatcg tcttgtgaag ctgatttacc agtaccgata gcccagattg gctcataagc
     1861 gataactgag gcagcaactt gttcagcagt caatccagcc aatgcagcag atacttgagc
     1921 acctacgaat tcagcagctt taccagcttc gtaagtttca agtgattcac cacaacagat
     1981 gattggaagc ataccgttcg caaagattgc ttttgctttt ttgttgatat cttcgtcagt
     2041 ttcatggaag tagtcacggc gttctgagtg accgataaca acgtaatcag taccgatttc
     2101 tttcaaaact tgtgggctag tttcaccagt gaaagcacct gcattttcaa agtagcagtt
     2161 ttgagcagca actttaaggt tcgagccttt tgcaacagca agaacagttg tcaaatcaag
     2221 agctggagca gcgatacctg cttcaacaag atctgatgaa ggaagttttg atgcaactgc
     2281 ttcaacgaat gctttagctt cttctggatt tttgttcatt ttccagttac cagcgataaa
     2341 tggtttacgt gacatttcac atacctcttt tttcaattta ttttctattt attctatcac
     2401 aattatacac agcttgcaaa tcttactcgg atttcaagcc tacttgcatg gattaatcct
     2461 tccagagatc catggcattt ctgaaatctg cagatacctg tgtcaactga ggattgcttg
     2521 cttctctttc tgctctcttg gcctcaattt gagccacact cttgattcct tcatggcgcc
     2581 agtttctcaa aattgcctga atgtacttcc agtttggttt tccattcaaa acagcttcac
     2641 gaagagcctc cttaatcaag tcagcactgg ttccatcttc ctttagtgtc ttggtcaaat
     2701 cctcaatctc aaaaggcgtc aacaatcgtc ccaactcctg ctggaaggtt tccaccaaat
     2761 ccttgagctg gttttgaggt gttagctggt ctgaacttga acgaactgct ccaaacaggt
     2821 catccaaacg ttccaaggcc aaactagcat caaagaacaa ttcaatttcg ccatttaatt
     2881 cgatagtacg atactggagc agtccccttt ccgtcagatt agaaatggac tggttgacat
     2941 ccgaaatttc cttgccaatc ctttcagcaa tctggcttgg cgacatttct tctaagcctg
     3001 tcgtattttg caaatagaaa aattgccaaa ccagaaaatc gtcgctagaa ggaaagagtt
     3061 ccttaaaatg caagagcagg gcactcggta aaaccaaggt acctgattta aaagcgtcta
     3121 aatatgtcat aattcctctt ataaataccc atatgcagat gcatcatctt ctatctttct
     3181 ccaatcaaaa ggcgtctcct gatagaccgc atggtttacc caattactga aaaagagggc
     3241 tgcagatgaa gaccaacaaa gacaaggtac ctgattaaca tcatcatctt taaagtaatt
     3301 ttctggaata tgtgggtcga aacctgcatc acgatctcga aaatactctt ttgccaaggt
     3361 atcacggtca tactccaaat gaccaaaact ataaatttct cgtaaatcac gactggccaa
     3421 aatagaaacc ccaacctgag gtccttctga taaaatctcg agattggtct tgtttaagac
     3481 ctcttcctta gaaatctccg tgtgccgtga atgaggggat acatagctat catcaaagcc
     3541 tctaaataga aggtgaccct cttttagggt gtcctgagga taaatacctg atagcttact
     3601 gtccatctgg tatttttcta caccatagcg cagataaagc ccagcctgag ccccccaaca
     3661 gatatgaagg gtcgaataga catgagtctt agaccactca agcatctgtc taaattcctc
     3721 ccaatagtcc acttcctcaa atggtaaatg ctcaactgga gcacccgtga tgatcatccc
     3781 atcaaaatac tcatccttga cttcaggaaa agttttatag aaggtctcca tgtgctctga
     3841 acgagttgtt ttagaacggt ggctctccat atagagaaaa tcaatatcca gttgtagggg
     3901 tgtattagcc aagtggcgca acaactgggt ctctgtgacc attttctgtg gcatgagatt
     3961 taaaattaaa atcttcaaag gacggatatc ttggtgggca gcacgttgat catccatgac
     4021 aaagatattc tctgtccgta aaatctcaac agctggcaat tttttatcaa ttcgaatcgg
     4081 cataaccctc tcctaactgt actctttcag gaataattgc atatgtttga agacaaatct
     4141 caaacactta gtccttttat tatactgtaa gaagatatag ttttcaatta tagtttttct
     4201 ctaactagtt atagtctatt tttatatcct agtgtagaga aaacagccct agggactgtt
     4261 ttcattaata atgcataaga actttgtagt cgtagtcacc aattttttca cggccgttca
     4321 attcatccaa ttcaacaagg aaggcacaac ctgccataac accaccaagt ttttcaatca
     4381 tctcgatagt tgccttaaca gttccacctg tcgccaaaag gtcatctaca ataagaacac
     4441 gttgacctgg cttaatggca tccgcgtgca tagtcaaggt atcgacaccg tactcttttt
     4501 catagtcagc agaaataact tcgcgtggca atttacctgg cttacgaaca ggcgcaaaac
     4561 caattcccaa ctcaaaggca actggacaac ccacgataaa tccacgagct tcaggtccca
     4621 cgatcatgtc gactttcttg tcagtagcat actgaacgat ttcacgaaca gcgtagctat
     4681 aagcatttcc atcagccatc aaaggactaa tatcacggaa ggtaatgcct tcctttggat
     4741 aattttcaat tgttgcaatg taatctttta aattcatctt tttctttctt tcaaagtttt
     4801 ttactctcta ttatagcata ttttttaaga aagaaaaaag gaaaagttaa cttcaataat
     4861 tatctaacgt tttgacgatt tataactagc catcgcaata aagcccaatt tctgtttatt
     4921 cttagcaaac attttataca tagttaaaaa ctgctttcta ttctcctttt tacaagcatt
     4981 tacacaaatt ttcaaagttc ctagcaaacc ttcgtcataa atcatacccg ataatttcat
     5041 taatgtcatt tcaccagtca atgctttcac atcacaataa cctgattcta tcatcacctg
     5101 ttcccaacca tcttgagtta aaggacctac atttacatga attgcttgtg ataattcctg
     5161 tctgatagac tctttagctt ccttaagaag cacatcatgt gtcaagagaa gacctccagg
     5221 ttttaatacc cttagatatt ccattacaca ttttttctta gcttgatcgg cttgcatagt
     5281 cagcatagct tcatttataa caatatcaaa actagcatct tgataaggaa gtttcattgc
     5341 atttgctctt tcaaaactga ttaaatgagc aacacctgcc gttccagcag attttttagc
     5401 cacttctaaa gcttgagcat ccatatcaat agcagttatc ttgcaaccaa aacgctgtgc
     5461 caactcaatt gctgtagttc ccctattaca cgcaacctct agtattctct tttcttttga
     5521 aaatcctcct tctgcaatta accaatctgt ggcacgcttt ccacctggac gtaagcgttt
     5581 tttccccaat tttgctaaaa acttatgacc tgcttctgac atttgaacac ctctgttttt
     5641 tcttcattat aacacaaaat gatattatct gacaattcta atctattatt tgataaacta
     5701 gaatcaaaag catacaagtc accataacta tagcataaat ttattattac ctctctagtt
     5761 cgaattcaat tgctcacgta gtttacgcat atagcttcac ttgtagatgg tacccacaaa
     5821 taatactcaa tggaaatcaa agagaaaact agaaaactag ccgtaagctg ctcaaagtac
     5881 agctttgagg ttgcagataa aactgacgaa gtcagtaatc atacgtacgg caaggcgacg
     5941 ttgacgtggt ttgaagagat tttcgaagag tattaaagag catcacaata tagatgcata
     6001 atatgacttc cctagcaaaa aggagagaag cattttgttt ctctccctag attaactgat
     6061 atctctccac tacaattgat aaagagatat cttttcttgt agtgctttga attttattta
     6121 ttttagtaga ttgttttttc agtttctaca tcgaagaagt gtgctttgtt caagtcaaat
     6181 ccaagctcaa ctgttgcacc tgtttgcaag tagtcacgag catcaacttt tgcaacaaac
     6241 tcgtctttac caacttggca gtaaaggtga gattctgaac caagcagttc tgatacagag
     6301 atagtcgctt ttacaacaca gtctgggaat gtttcaagga aagcaggttc tgcattcacg
     6361 tcttctggac ggataccaaa gatcaattct tttccttcgt agcctttttc acgaagaact
     6421 ttcaatgctc cttctggcac tttcaaacgg aaaccgtcag aaacaatttc gctaccaacc
     6481 aatttcacgt tgatgaagtt catagctggg cttccgatga atcctgcaac gaatttgtta
     6541 actggatttt tgtaaacttc ttgaggagta ccgatttgtt ctacacgtcc gatagtacct
     6601 gtaccagcag ggttcttagt agctgacata ataacgatac ggtctgcaag tgtcatcgct
     6661 tctgtttggt cgtgagttac atagatagtt gtagctccga tacgacggtg aattttagcg
     6721 atttcagcac gcattgatac acgaagtttg gcatccaagt ttgacaaagg ttcgtccatc
     6781 aagaatactt tcgcatcacg gacaatcgca cgccccatgg caacacgttg acgttgacca
     6841 cctgaaaggt cagctggttt acgttccaag aattctttca atccaagtat ttcagctgct
     6901 tcttgaacac gtttgttaat gtcttctttg ctgtatttac gcaatttcaa accgaaagcc
     6961 atgttgtcat aaacagtcat gtgtgggtaa agagcgtagt tttggaatac catggcgata
     7021 tcacggtctt ttggagctac gtcgttgaca accacgccat cgatagatgc agtaccttct
     7081 gtaatgtctt caagaccagc aatcatacgg agtgtagttg atttaccaca tcctgaaggt
     7141 cctacgaaaa cgataaattc tttatctttg atgttcaagt tgaaatcttc aactgaatag
     7201 tgttcgctgt ttggatattt tttgtaaata tttttaagat tcaattctac catgaggtga
     7261 actccttttg tcttttgata attatattat agatgaaaac gctttatatt tctatggcaa
     7321 gatgaccaaa aaaataaaaa agttctgtgc aacttgcaca aaactttaga gaatatcgga
     7381 gaatttgaag aagataatct ttattattct tctatagaca aaagcagaaa caaggatcag
     7441 ttttcccata ttttcgctga ttctccacta catttgactg gattctaatt ttttagagaa
     7501 atacaaaaga gctagcttta gctagctctt ttcctatgcg gagagaggga cttgaaccct
     7561 cacgacctaa agcggtcaca ggatccttag tcctgcgcgt ctgccaattc cgccatcccc
     7621 gcgtcgatta ctttactagt atatcaactt ttgggatgct tgtcaacact ttttttcaaa
     7681 ttttttcatt ttcaccaacc aggttactca gaaagttcat ttagattttc atctactaac
     7741 ttagctccga gtgtattttt gaaatgacct agggcaaatt gatgattttc aggccagatg
     7801 gaagcaacag ctggtttaac aatctcgatg tcatatccta gattataggc atctatagct
     7861 gtatgtagga cacagatatc cgtcaagaca cctgttaaga taacggtaga cactctacgc
     7921 tctctcaaac gaatatctag gtcagtccct gaaaaagctg agtaatggcg tttatccatc
     7981 caaaagacac gactgtctga accatgctct tgataaaaga tccccaaatc tccatataaa
     8041 ttccgtccac tcgtcccaat cagattatga ggaggaaata acttactttc cggatggaaa
     8101 caatcgtttt cttcatgagc atcaatagta aagaagatat aatctcctcg ttcaaaagct
     8161 aatcgagtta ccttgctgat ggcatccgaa atcgcctgag ctggagcacc tgctgttagt
     8221 ttcccactat cagcaacaaa atcttctgta taatcaatcg aaattaaagc ctttgtcatt
     8281 agtaatctct tttcttcact tcttcaaaaa tatctgaaat caagacctta agataggttc
     8341 ccttcattcc aagtgagcga ctttcaataa tccccgcaga ctcaagttta cgaagagcat
     8401 tgacaatcac agagcgagtg attccgatac gatctgcaat cactgacgca gtcaacttcc
     8461 cttcatttcc atttaattcc cctaaaattg ctgaaacagc acggagttcg gagtaagaaa
     8521 gggtattgac cgccatggtg acagcagtac gacgacgaat atttttctca tcttcttcac
     8581 gttggaagtt aagaagctga atcccaacaa cggtactggc aatctcaaca agaaccaagt
     8641 cctcatcttc gaatttttta tcattacgcc aaataatcaa agaaccaagg cgaatccccg
     8701 atacatgaat cggtgcaata gtcgtcaagc catctggaaa atcatctcta ctctcaacag
     8761 ggaaaatact catatcatgc tcaacaggta agtttgcttc tgtttcgtaa atcatattag
     8821 ccccttgaac gtagtcatct gggaaaatct tagtttggaa gaattgctct acgcgatctg
     8881 tatttgtttt ataacgcata aaatagccaa gcagacgtcc cttactattg ataatgcagg
     8941 cattgcaatg aataatatcc gctaactgac gcgtaatagc gttgtaagga agctcatcct
     9001 gcaactgctc ctctgagcgt ttcaaaattg atgtaatttt tctagttttt tctaataaat
     9061 gtgccatttt tcacctcgaa
//
LOCUS       DAVLTM010000076         8926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLTM010000076 DAVLTM010000000
VERSION     DAVLTM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.077"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(328..1176)
                     /gene="dprA"
                     /locus_tag="VYN16_001519"
     CDS             complement(328..1176)
                     /gene="dprA"
                     /locus_tag="VYN16_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1404027.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1282..1971)
                     /locus_tag="VYN16_001520"
     CDS             complement(1282..1971)
                     /locus_tag="VYN16_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1404028.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1983..2861)
                     /locus_tag="VYN16_001521"
     CDS             complement(1983..2861)
                     /locus_tag="VYN16_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1404029.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2851..3720)
                     /gene="licA"
                     /locus_tag="VYN16_001522"
     CDS             complement(2851..3720)
                     /gene="licA"
                     /locus_tag="VYN16_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1404030.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3737..4759)
                     /locus_tag="VYN16_001523"
     CDS             complement(3737..4759)
                     /locus_tag="VYN16_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1404031.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4764..5471)
                     /locus_tag="VYN16_001524"
     CDS             complement(4764..5471)
                     /locus_tag="VYN16_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1404032.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFVDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5807..7294
                     /gene="tacF"
                     /locus_tag="VYN16_001525"
     CDS             5807..7294
                     /gene="tacF"
                     /locus_tag="VYN16_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1404033.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRVISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7304..8107
                     /locus_tag="VYN16_001526"
     CDS             7304..8107
                     /locus_tag="VYN16_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1404034.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8109..8918
                     /locus_tag="VYN16_001527"
     CDS             8109..8918
                     /locus_tag="VYN16_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1404035.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKYDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2789 a         1806 c         1507 g         2824 t
ORIGIN      
        1 tcctgataga gttgttcaca tcttatttca attcactata ctttccctta tactcaatga
       61 aaatcaaagc actgctttga ggttgtagat aagactgacg aagtcagctc aaaatactgt
      121 tttgaggttg cagatggaag ctgacgtagt ttgaagagat tttcgaagag tattatatga
      181 gaaaaatcct ctactctact aatccactta gaaaaaacgc atattatcaa gtgttaaaaa
      241 cttgctaata tgcgttttat tatggaaatg ttgggttcat tttctcttaa aattgatttt
      301 ttcttagaat tctagcttag gtcattttta aaattcaaat tccgcaagaa catcttgccc
      361 actggtgacc aattttgctc cttcttgaat caaatgatgg caaccgtctg atagtccatc
      421 taaaatgcta ccaggaatag caaagacatc gcgtccttct tccattgctc gctcacacgt
      481 aatgagacta cctgaacgca tcttagcctc tgctacaatc acaccacgac aaagtccagc
      541 aatgatgcga ttacgggcag gaaaatgaaa tttcagaggt tgttcgccag gtccatattc
      601 acttagaacc agatggtcat tgccgatgta gtcttgcaag cgtttattgg ctttaggata
      661 aaacacatcc agtcctgttc caatcactgc aatggttttt ccgccattct gaagagctgc
      721 catatgagct gctgtgtcaa tgcccttggc cagaccactg acaataacca gttcattttc
      781 caagccttga atgacttttt caactgactt agctccctgt ttgctacaag cacgactgcc
      841 cacgaccgct accttcggga atttcaggag gtcaagattt cccttgtaaa ataaaagtac
      901 aggcgcatca tatatttcac tcaaatccca aggataacag tcatctaaaa tagagaaaga
      961 tggaaatttt tgaaactctt tcgacaaatg cgcatcgtct atctgaaaat aacgttccat
     1021 aaaaacggct ggattacggc aacctgatat atctgcaata tcacccaaca aaagctcctg
     1081 atcaacattt tcaccgtatt ctagcacttt caaaatctgt tgattggtca aacctgattt
     1141 ttttaactta tagatttcat agtttgtgat tttcataaat aactccattt ctttttctac
     1201 tcatctatct attcgtaaaa gttcgtaaaa gttcgtaaaa aatcaaaaaa catccgacta
     1261 ttttgccaga tgttggaatc tttaattttc gtttttaaga atttcttcta atttacgata
     1321 gtcttggaca ctatcgatct cataaatgct attgccttct aattcttcaa catagacatc
     1381 tagctctttg atattatcct taaccatatt gtcccaatag agatcaacaa attcaccact
     1441 tgcataagcc ttgtcgataa agctgacaat cttttctgca gttggagcat cccagaagga
     1501 tacaccacta aggatgcgac ctgccttgct atcaacaata atgtcttgaa ccttgtagtc
     1561 atctccatag accaagaacc attcgttggt acaatcttca cgataaacac taaaataagt
     1621 cgaacgtgtc aaatcattgc ggaacatatt tttaaagaga taattgtcag catcaataac
     1681 atagctgttg gccaattctt cttttacaag atagagagag taaaagttat tgtagtcagc
     1741 gtatttatca ttgaaaacga ggcgaacacc gtatttttct tttaagtaat cgaattgttc
     1801 tttaagataa ccaacaatga tgatgatgtc attgattcct ttttctttga gaaactcaat
     1861 ttggtattca atcaaaggtt tttgattaac ctgaaccaag gctttagggg tattttcagt
     1921 cataggacgc aagcgagttc ccaatcccgc tgctaagata atggctttca cacgaatctc
     1981 ctttattctt taataataat ataaactcca gcaatgacga caagtgacgt cataatggtc
     2041 agcatatcta gcggtgcacc caaaaaaaca actgcaaaca agaccgtcca tactacatag
     2101 ctcacgttca agcctgtagc cttggctggt tgcaagcgat tgatagcgat ataataagcc
     2161 aagtaggaaa tcatatcaaa ggctgcaaaa acaatcatga gacctagcaa ttgtccattg
     2221 gctactgcag taaatgactg atgagagaag agcacaatca caagatagga caagaacgaa
     2281 gtcacttgac ggattaagag ggcttcgatt tcactcagtt cactttccat agcaaaggag
     2341 ctaagaacac tctcacttcc ccatgcaata gcacaaacca aagcacagag aatcccaatg
     2401 tagaaggaat taacctgttc aaccttatag gtctgggcaa taatccctcc aataatcaag
     2461 acaatcccaa atacagtatt tttcgaaatc ttgtgcttca aaaagaagaa agccaataga
     2521 actgaaatcg cagggtaaat agccgataca gatgaagcta aagaacttcc gatatactta
     2581 actgcataaa gattggcctg cataccgata gggcctgcta gcaaggctcc gatgataaca
     2641 ctgacattgc gaatatttaa gaaaattgag aggcgaactt tcccttcttt taccaagaga
     2701 aaagctagta agataaagat gctcaaaaaa tcatgagctg cagccaccac aaagggtgac
     2761 aaatctgtaa aaatcgaaaa gatataagca ctaaccgtta gacccaagcc ccagaaaata
     2821 cctgagagaa ggccaaaagg aactccgttt ttacttttca tctgaacctc cataagaagc
     2881 caaaccttta acagctcttt ggtaacgatt cacaccatag tcaccaaaat cttcaccttg
     2941 ctcttcctta tagacagtcc atagactcca aatagtatct tgtaaaattt tataaatagc
     3001 aatcttttca tgagaaaccg gtgtttggtc actctcatag tgagataaga aagtttcttc
     3061 ctcttgggaa gtgaattcag actctaaaaa gagggcagcc aaatcccaca ttggatcatt
     3121 cattgatgaa tattcccagt caatcaaata aagtcgtcct tgaggtgatt cgataaagtt
     3181 ttcaggcacc aaatcgatat gacaagattt tctgtcaaca cctaagtcag ccagtctttt
     3241 ctctaaggag aagactgcat ttctaacaga ttcatagttg gcataaggaa tttgttcttc
     3301 aatcaaggat tcgtattttt tgatttcttc aaaaggagca aattctcctc ttaattcctt
     3361 agcagacgta tgaatagttt gtaatattgg agcaattttg tcgaacttgg tcttgattga
     3421 cgttgaatca agcgtaatcg cagattcgat atactcattt actttgatac cagcttcaat
     3481 atcaaaaaga taatttttta catctaagcc taaatccttt agtagttcaa gattgtactt
     3541 ttcatcttgt cgattgataa gcttttctgt ccctttacca aagaatttaa caatgtattg
     3601 cttatttgtt gttttggcca aatagttttg attggtcatt ccacccagtt gttcaacact
     3661 gaggacttcc tcttcttgac taagtaagga agaaattttt tctttaatga ttttctccac
     3721 aattaacctc cagtacttat acttcccact taaacactgt tttaaaggct gtgtttaaat
     3781 cggttgcaaa gacacgatga atatctttaa tttctcttac aggttcttct agataaagga
     3841 tatttttaag acgattggca aatttcttga cttccatcat ttggatagca tttttaaaat
     3901 caatgcgacc agaacgagat gacccaacca aaatcaagcc cttttctaag gcatcgcgag
     3961 tattgagatt gactttatat tcgctaactc ccatcatgag aatcgttccc tgaggacgaa
     4021 tgtagcgaat caagtcatta atagctggtc cagtaccatc accaccacaa cattcaaaag
     4081 catggtcaaa ggccaactct tcaggaatat tatccgtaat atagcattct ttggcaaatg
     4141 agaagagttc caacttttcc caatgacgac caataaccac aatctctgat tctggcaaag
     4201 tatagttgat aatattggca accacaaaag ctaaacttcc atctccaata acggcgatcc
     4261 gctcccgctt gctatgagca agagtcaata gacgattcat agcgtgcatg cccacactga
     4321 caaactctgt aatggctgca accgtatctt caatagcatc ataagccacc acacgatctt
     4381 tagggagaga aacaaactct ctcataaagc catcaaatcc actagacaag aaatgggtcc
     4441 ctgtcatgta gttttcatag aattcttcat cactctgcat aggagactga ttgggaatca
     4501 tgacaacttt ttgaccaacc tcgtaggttc cggtcgggtc agaaatgacg gttccacatg
     4561 actcgtgaat cattgccatt ggaagctttt tattcaaaat cttgggatca cgttttccct
     4621 gatagtaacg ctgatccgca tgacagacag ccatgtagtt gggacggata aggatatgat
     4681 tctcttggtc aatagcctct tcctgatatt tgacattgat aaacttaggc ttagttagtt
     4741 gataaatttg attaatcatt ttactagtct ttctcaatca tactttttgc aatcttcaaa
     4801 tctgttacgg ttgtaatctt cagatttgag tattcacctt tggccaaggc cacatctttt
     4861 cctttgatca caaagatttt acatgcatct gtcaagattt ccttctcttc atcagaaaga
     4921 gatccataaa ggtccacgaa gtccttgcaa cggaatgttt gaggtgtttg tccttgataa
     4981 aggtgagcac gatttggaat atctgtaata aattgaccat tggtactttc aacgatagta
     5041 tcaactgctt ctaccactgt gtccactgcg tcatgatttt gggcaagttg gatattgtcc
     5101 tgaatcatgc gaagtgtaat aaatggacga acagaatcgt gggtaacaac gatatcctct
     5161 ggagtaagcg gacgataagc atcaatggct tcaatgattt tcttaatact tgtattgcgg
     5221 tcagcaccac cctttgtaat gatgatacgt tccttataaa gaggaagata tttatctaca
     5281 agatcttctg catgagaaac ccagtctcca tgaacaccaa ctacaatttt ttcaatactt
     5341 ggctccaaga caaatttttc aattgtatga atcaaaatag gtcgatcacc tagctctaaa
     5401 aattgttttg gcaagttact gatccccatg cgtgtgccag ttccaccggc aagaattcct
     5461 gcataaatca tctattttct cctttgtctt actaaaaaaa cttattctct ctattatacc
     5521 acaatctagt cctatcataa aagaaatact ggtcctcccc ttctagaata ggagaattat
     5581 gcagttctat tattcaaaga aactagtcca tttctagtaa tgactaggaa ttctgtagca
     5641 attactaata aatggctgcg aaattttaga gtccttcaga ataatatact ttccttaaag
     5701 aaaacttaaa acttaagatt tgaaacttca aagtgattac ttgacttact gcccttcatt
     5761 tcttattagc taactttatg atataatgaa aaacgaggta aattgaatga aaagtataaa
     5821 attaaatgct ctatcttaca tgggaattcg tgtcttgaat attatttttc ccatcctaac
     5881 tggaacctat gtcgcgcgtg tcttggaccg aactgactat ggttacttca actcagtcga
     5941 cactattttg tcatttttct tgccctttgc aacttatggt gtctataact acggtttaag
     6001 ggttatcagt aatgtcaagg ataacaaaaa agatcttaac agaacctttt ctagtctttt
     6061 ttatttgtgc atcgcttgta cgattttgac cactgctgtc tatatcctag cctatcctct
     6121 cttctttatt gataatccaa tcgtcaaaaa ggtctacctt gttatgggga ttcaactcat
     6181 tgcccagatt ttttcaatcg aatgggtcaa tgaagctctg gaaaattaca gttttctctt
     6241 ttacaaaact gccttcatcc gtatcctgat gctggtctct attttcttat ttgttaaaaa
     6301 tgaacacgat attgttgtct atacacttgt gatgagttta tcgacgctga ttaactacct
     6361 gattagttat ttttggatta aaagagacat caaacttgtt aagattcacc taagtgattt
     6421 taaaccactc tttctccctc tgacagccat gttagtcttt gccaatgcca atatgctctt
     6481 cactttttta gatcgcctct tcctcgttaa aacagggatt gatgtcaacg ttagttacta
     6541 taccatagct cagcgaattg tgaccgttat agctggggtt gtaacaggtg caattggagt
     6601 gagtgtgcct cgtctcagtt actatctggg gaaaggagac aaagaagcct atgtttctct
     6661 ggttaataga ggtagtcgaa tctttaactt ctttatcatt ccactgagtt ttggactcat
     6721 ggttttagga ccaaatgcca tcctacttta cggtagtgaa aaatatatcg gaggcggcat
     6781 cttgacctct ctcttcgctt ttcgtacgat tatcctggcc ttagatacca ttcttggttc
     6841 ccaaattctc tttaccaatg gctatgaaaa acgtatcaca gtctatacag tctttgctgg
     6901 gctactcaat ttgggcttga atagtctcct ctttttcaac catatcgtgg ctcctgaata
     6961 ctacttactg acaactatgt tatcagagac ttctctactt gttttctata tcattttcat
     7021 ccatagaaaa caactcatcc acttgggaca tatctttagc tatactgttc gatactctct
     7081 cttttcactt tcctttgtag caatttattt cctgattaat ttcgtgtatc ctgtggatat
     7141 ggtcattaat ttgccatttt tgattaatac tggtttgatt gtcttgctat cagctatctc
     7201 ttatattagt ctacttgtct tcacaaaaga tagcattttc tatgaatttt taaaccatgt
     7261 cctagcctta aaaaataaat ttaaaaaatc ataggagttt aaaatgaaac aactaaccgt
     7321 tgaagatgcc aaacaaattg aattagaaat tttggattat attgatactc tctgtaaaaa
     7381 gcacaatatc aactatatta ttaactacgg tactctgatt ggggcggttc gacatgaggg
     7441 ctttatccct tgggacgacg atattgattt atccatgcct agagaagact accaacgatt
     7501 tattaacatt tttcaaaagg aaaaaagcaa gtataagctc ctatccttag aaactgataa
     7561 gaactacttt aacaacttta tcaagataac cgacagtacg actaaaatta ttgatactcg
     7621 aaatacaaaa acctatgagt ctggtatctt tatcgatatt ttccctatag atcgctttga
     7681 tgatcctaag gtcattgata cttgttataa actggaaagc ttcaaactgc tgtctttcag
     7741 taaacataaa aatattgtct ataaggatag ccttttaaaa gattggatac gaacagcctt
     7801 ctggttactc cttcgaccgg tttctcctcg ttattttgca aataaaatcg agaaagaaat
     7861 tcaaaaatat agtcgtgaaa atgggcaata tatggctttt atcccttcaa aatttaagga
     7921 aaaggaagtc ttcccaagtg gtacctttga taaaacaatc gatttaccct ttgagaattt
     7981 aagccttcct gcacctgaaa aatttgatac tattttgaca caattttatg gagattatat
     8041 gaccctacca ccagaagaaa aacgcttcta cagtcatgaa tttcacgctt ataaattgga
     8101 ggattaggat gcaatattta gaaaaaaaag aaattaaaga aattcaacta gccctgctgg
     8161 actatattga tgagacttgt aagaaatatg atattcctta ttttctcagt tatggaacca
     8221 tgcttggagc catccgccac aaaggtatga ttccttggga tgatgatatt gatatttccc
     8281 tttatcgtga ggattatgag cgtttactga agattattga agaagaaaat caccctcgct
     8341 acaaggttct ttcctacgat acatcttctt ggtacttcca taatttcgca tcgattttgg
     8401 acacttctac tgttatagaa gaccatgtta agtacaagcg tcatgatacc agccttttca
     8461 tcgatgtctt cccaattgat cgatttacag acttgagcat tgtcgacaag agctataagt
     8521 atgtggctct tcgtcaacta gcttatatca aaaaatcacg agcagttcac ggtgatagca
     8581 aactaaaaga ttttcttaga ttatgtagct ggtacgctct ccgatttgtc aatcctcgct
     8641 acttttacaa gaaaattgat caactagtca aaaatgctgt aaccaacact cctcaatatg
     8701 aaggaggagt tgggatcggt aaggaaggga tgaaagaaat cttcccagtt gataccttta
     8761 aagaactgat tttaactgag tttgagggcc gtatgttgcc tgttcccaaa aaatatgacc
     8821 aatttttaac ccagatgtat ggcgattata tgacaccacc atcaaaagaa atgcaagagt
     8881 ggtatagtca tagcattaaa gcttatcgca aaaactgatt gagggg
//
LOCUS       DAVLTM010000077         8881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLTM010000077 DAVLTM010000000
VERSION     DAVLTM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.078"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(390..2669)
                     /gene="pulA"
                     /locus_tag="VYN16_001528"
     CDS             complement(390..2669)
                     /gene="pulA"
                     /locus_tag="VYN16_001528"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1404036.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(2781..4739)
                     /gene="ligA"
                     /locus_tag="VYN16_001529"
     CDS             complement(2781..4739)
                     /gene="ligA"
                     /locus_tag="VYN16_001529"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1404037.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTNLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(4832..6010)
                     /locus_tag="VYN16_001530"
     CDS             complement(4832..6010)
                     /locus_tag="VYN16_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1404038.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLQSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(6090..6953)
                     /locus_tag="VYN16_001531"
     CDS             complement(6090..6953)
                     /locus_tag="VYN16_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1404039.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGLALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(6956..8857)
                     /locus_tag="VYN16_001532"
     CDS             complement(6956..8857)
                     /locus_tag="VYN16_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1404040.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKVTAEMSQTEEASTSNQAKEASPVNDYQVQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         2663 a         1960 c         1603 g         2655 t
ORIGIN      
        1 taatgtggtt tgaagaaatt ttcgaagagc gtgaatgagt atcatctata gtaaaataaa
       61 aaaactgaac aatttggttg gggacagcca aaccaatttc tcacaatgtt ttagaaacaa
      121 gggtgtgcta ttccaatttc aggctactat aactgtcata gattgctgaa acaaagtcta
      181 ggtaaaagtc ttcataataa aaagaccccc tatcaagtgt tcaaaaactt tgataggagg
      241 tcttgttttg tgaaaatatt tatcaaattt tctatacaag tgagctgtta gccaggttct
      301 ttctattctt tcaatttcaa tgaatggatt ttttactaat actcataact gggaatttgt
      361 ctgtgtaaaa atagcgagat agatggtatt tataaaacac tcaagacagc tagactaata
      421 tcatttaaaa cattatcttc ttttgagcga ctgttggtta ccaacatagc taaatttcct
      481 gcattttcaa attgataggg ttctgattta gcattcacaa ccaccaagag atgttctttg
      541 ccgtgaactt catagataag gtagccgcta tgttcaatcg cagaatgcac aaagacatga
      601 tggtaaattt catcatagct agagtaagaa aaggcaccag tttttgtctt caatcggatg
      661 acttgacgga taaactcaat actgtcttga cgctcattaa tcaagttcca gttcacttgg
      721 ttcacactgt caggagcatt atagctattc atcgcacgct ctctatcatc atgggtcaac
      781 tcaccatttt caccagtcgc aaccagtttg gtacgaccaa attcttgacc gatttccata
      841 aaggccatcc cctgcatgag cagattcatg gctgtggcag tttcgacctt gcgcatgatt
      901 tgctctgaac tttggtctgg atgaagggtt gccaataaat cgtgaagatt gtaattgtca
      961 tgggcttcta catagttaag cacctgattt ggatgtgtat agcttcctaa ttcacgactt
     1021 cctaggattg ctttagctag aattggctct gtcgcagcac cactgacaaa acctgacttg
     1081 atagcaccat aaacttctcc ccctttgaca gcatcgcgct gattgtcatt aaagaaacca
     1141 atatttggca tctggtaggc attgtccttc ttggccttat cataaggggc aagacctgtt
     1201 cccatatccc atccttctcc atagaggata atgttggagt cgatttcatc caagctttga
     1261 cgaatcatct gcatggtctt gacatcatga atccccatca agtcaaaacg gaagccgtca
     1321 atattatatt cctgcaccca gtatagaaga gaatcaatca tatacttgcg aaacatttcg
     1381 tgttcactgg ctgtttcatt tccaacaccc gttccattct ggaaggtacc atctggattc
     1441 atacgataat agtaatcagg gactgttgtt tggaatggtg catcaacaac tgagaaggta
     1501 tggttataga ctacatccat aatgactcca atacccgcat cgtgataagc ttgaaccatc
     1561 accttcaaat cacgaatgac ctgagctgga tcatctggat tagttgaaaa actagtttct
     1621 ggcgcgttat agttttgtgg atcataaccc cagttgtagg ttacatttcc atcctcatcg
     1681 tattctttat gacggtctgc aattggttgc aattgaacat aattgtagcc cagcttcttg
     1741 atgtaatcaa aagcagttga ctggccgtat tggttaactg ttccagcctg agcagcaccc
     1801 aagaaagttc ctcgaagatg ttcatctaca cccgatgtag gtgatttagt caaatcacga
     1861 atatgcattt cacagataac tgccttacat ggattttcca agcgccaagt agcctccgaa
     1921 ccgtgcttaa cctcgaagtt ttcaacttgc ttttctacat ggctcagaat agctgaacgt
     1981 ttgccatcag ggctggtcgc gattgtataa ggatcacgtg tcagtgtttg gtgatgaggg
     2041 aattggactt gatactgata agtcttacct accaaatctt cttcaacatc caaactccag
     2101 acaccgattg tattgtcctt atgattataa gagtagctat tgcctctttt catctcaaaa
     2161 gtcttccaaa cgggtgcatc attagcagct gattcataaa cgacaacttg cacttctgtc
     2221 gctgtaggtg accagaggga aaaatgagcc tgattgtcct ctacacggca acccaattct
     2281 ccttggtaac cccaatgatg atcaaaacta gcactgttaa tggccttatc aaaggcaaaa
     2341 ggattttgat ttttatagaa aggactggca atagcaggat tttcagagta ataaatccta
     2401 tcatcgcctt ccaaaatcca gacctctgtt aataggggat agtgattaaa acggatagaa
     2461 tattctttac tagtttgacc tgtatgaacc acaaaattca agctttctat aacatgtgaa
     2521 cttgggtgtt caaagctaaa taaagctcca aaataatctt ctttgtaggt tagcaaatca
     2581 attcgttgat cctgactttt tacaaaggag caagtgtcat attctccatt cttacgatgg
     2641 taatgaatgc gcatagggta gttatacatt ttttattttt cctttttact ttgtttctat
     2701 ttcactaata aatttttgtc aatctcgtct caattaacag acatagtcat attctctaaa
     2761 ctctgttttt aaacgatcca ttacaaactt tctagccatg cctcatctct gacctggata
     2821 ccaagttctt gtgctttttg cagcttactt ccagcgtctg cacctaccac gacgaggttg
     2881 gtctttttag aaacactacc tgtcactttg gcacccagac tttcgagttt acttttagct
     2941 tctgagcgct tgagtcgttc caattttcct gtcaatacca cggtcaaacc tgacaaggcc
     3001 gcatccgcta ctaccgtctg tcctttatag tccagattga ccccagtttc tttcaattct
     3061 ctgagcagaa tttcagagcc ttctgtcgca aaataagtct gaagactttt ggcaatcacg
     3121 ccacctagac tttcaatact agccacttcc tctgaatctg cctgatacag attttcaatt
     3181 gaatggaaat attgaagtaa aagctgacta gccttgcttc cgacatgacg aattcccaaa
     3241 ccaaataaga gcttctcggc agaattttcc tttgacgctt ggatagcctg atacagttta
     3301 gcagcggact tttccttaac cccctctaaa aggaggaaat cctcttcttg caaacgataa
     3361 atatccgcca catccttgac taaattagca gcaaaaagct tctcaacaat agatggacca
     3421 aggcctgtaa tattcatagc atcacgagaa gcaaagtgaa tcaagccttc catgatttga
     3481 gcagggcaac gcggattgat acaacgtagg gccacttcat cttcaaagtg caacaagtca
     3541 gagttacaac ttggacagtt tgtagggata tctagttttt cttcagaaac ccgtttggac
     3601 tctaccacac gtaaaacggc agggatgatg tcaccagcct tatatacaat gaccgtatcg
     3661 tcttttcgga tatctttttc agcaatataa tctacattgt gcagggtcgc acggctaaca
     3721 gtcgtaccgg caagttgtac tggtgttaga ttagcagttg gagttacaac accggtacgg
     3781 ccaactgtcc agtcaactga taagagttga gcttcttttt cttcagcagg gaacttgtag
     3841 gctactgccc actttggagc cttaacggta aaaccaagtt cttcttgact tgctaggtcg
     3901 ttgaccttga ttaccactcc atcaatatcg taaggtagat tttcccgttc ctgtcctact
     3961 tcttggataa aattccagat ttcatctatg ttttcagcca agattcgctt aggattgacc
     4021 acaaaaccta gttgttctag gtacttcaaa cccttttctt ggctatcacg agttgaaggg
     4081 ctggcttctt gatagagaaa cgttgcaaga ttacgcttgg caactactgc tgtatccaac
     4141 tgacgcagag ttcctgctgc cgcattacga ggattagcaa attcaggctc tccattttct
     4201 tggcgcgctt ggttaacttg gtcaaaggaa gcgcgtggca tgtaacattc cccacgaact
     4261 gtgatatcta gttcttctgg caaagtcaaa gggatgtcct taacacgctt gaggttttct
     4321 gtgatatttt caccaattga accatctcca cgtgttaccc cagcaaccaa aatccccttt
     4381 tcataagtca gcgagataga taagccatcg attttcagct cacaaatata ggtcggatga
     4441 gccacttcct tacgaacacg cgcatcaaaa gcatctagct cctcacgtga aaaagcatcc
     4501 tgcaaactat aaagaggata ctgatgactg tatttttcaa aaccatctaa aaccttgcca
     4561 ccaacacgat gagtcggact gtctgctagc acttgctctg gataagcagt ttctaactcg
     4621 accaactcac ggtaaaggcg gtcatactca ctgtctgaaa ccgagggatt atcgctggta
     4681 tagtactcag tcgcatagcg attgagcaaa gcgactaact cattcattct tttattcata
     4741 agaccatttt accataaaac aagccctcct cacaaacgag aagggcggaa aaaacactta
     4801 gtttgaaatt atttttgaaa ctcaagcaac cttatatcaa tttttcaaaa tgagttcgaa
     4861 cataaataaa cgatatacaa gacaagatga taacaccact tccaattatc aggaaagaag
     4921 agagatgtac actttgcaag actgtcataa atccttttgc aataggcata aatagaatag
     4981 ctaaggtaaa aattgtactc agtactctcc caagaaattc gctctcaacc ttggtttgta
     5041 cttgagtaaa aaagtgaata ttaaaaatcg tcataaacaa ttcacaaact aaatttccag
     5101 aaaaggaaag aaaagttgga agtggtaatc ccatcataaa aactccaaca cctgtcaaag
     5161 ccagtaaaat caaaagatta taaatattag ctttaatttt actagctaga agagccccaa
     5221 tgatggaacc aatagccccc atagttaaaa tacttgcata ggctccttct gacccgtaaa
     5281 gctgattcga aaagggaagt agaaattcaa aagctgcaaa aaagaaatta acgctggaag
     5341 ctaccagcaa aaggaagaaa atttcttgct gatgccagat atagtgtaac ccatccttga
     5401 tatctacaaa aatatctctc ccagtaaaag cctttttctc ttgaactttt gcttcctctt
     5461 ttggaaggaa agccactaga acaaaagcaa tgaaaaaagt cagcgagtct agcagtagcg
     5521 tcatatggag acttgcaaac tgtaaaacaa ggaaggaaag aacaggagag ctaacaccta
     5581 caacctgcaa aaccagctct aagcgagaat tatagatcac aatctcatct ttctccacca
     5641 cttcagttat gatagcttta ttggctgtgc gagaaaaggc aaaagcaata gcctgcacaa
     5701 tgttagcaac aatcaaagcg ccaatcatcc agctatcatt ccttatgaaa gaaatagcca
     5761 gacaaagaat cccacaaaca agatctgccg tcattaaaat cttacgacga gaaaaacggt
     5821 ctgaaataac tccaccaaag ggattgacga gaatagatgt gacgagctca gaaatctgat
     5881 acattcctaa aactgtctgt cctatagtcc ccatagaagc caaccagaca ctatttccat
     5941 aatcatagag catatttccc attttattga tagccccacg gctaatcaac tgcactgcat
     6001 agcgattcat attaaagctc ctctcaaatt ttgaaactat tgtatcaaaa ccgaaaggag
     6061 ctttttattt tttcccttat ttgggaaaat taacttttga caaatttttc gtagtgttcc
     6121 tgataatagg ctacttgctc tggaagacct aacacatcaa aaatatgcat ggcctcttgc
     6181 atctgcttac agccttcttt acactgtcct ttttgatata aggccaaacc ttttaaataa
     6241 tggaaaacat tacgctcata aagcttaata cctttgtcaa taatcttctc tgtataagcc
     6301 tcaaaatagt tggcattata aaaagaagaa tgctctaaac aatgctggta acaattgagg
     6361 gccaaaatta acactaatct cttatggcga ctaatctctt ggtaaaattc ctccctctcc
     6421 ataacttctc taccaatccg agtgacatag tctacatcgt agaaactata gaggttaccg
     6481 aaaagaatca actcatacat ggtccattct tctgttttga agagataatc tgctacctta
     6541 tccaaatcat cctgcttcat atcataactc gcatctcttt gacaaatcag accttgtagc
     6601 aaaatccagt tcagctcaaa ataaagggga gtcgtcgaac tcttagactt ttcaagttgt
     6661 tctctttgaa gcttttgaaa acctgcaata tcgtttgaat agtaaagtgg gataatctgt
     6721 gccatcatag acacatgttc atgattatga aaattccttg ccttatccat gaaattttcg
     6781 attgttacat gaatgttatc caaaatctca aagaaacggg agactgccag gtcagactcc
     6841 ccaagctcaa agcgagataa ctgagaggta gagcaggatt cgcctgctgc ttcctttaaa
     6901 gaataatttc cacttgttcg aaattcacga aatacttttc caagatgttc catctttaca
     6961 cctgctctga taattcttcc cactcaagca tagcttcttc ctgacgatgg ctgattttgt
     7021 ccagctcagc ctgtaattcc atgagtttgt cggcatcgtt tgtttccaac atttgttcag
     7081 aaatggcttg gctttgactt tctagctctt caatttcagc ttctagactt tcgatttgtc
     7141 gcatgagttt gcgaacttct ttttgacttt ctttctggac ctgatagtca ttgactggac
     7201 ttgcttcctt tgcttgattg ctagttgaag cttcctcagt ctgactcatt tctgctgtta
     7261 ctttcttctc aacatagtag tcgtaatctc caaggtagag agttgaacca ttctcagaca
     7321 attccaaaac atgagttgcc acacgattga taaagtaacg atcatgactg acaaacagca
     7381 aggtaccatc aaagtcaatc aaggcatttt ctagcacttc cttactatca atatccaagt
     7441 ggttggtcgg ctcatccaga atcaaaaagt tattgttttc catagacaat ttagctaaaa
     7501 gcaaacgagc tttttcgcca ccagatagca tgccgactga ttttttaaca tcatctcctg
     7561 agaaaaggaa ggctccaaga cggttgcgga tttcaacttc tggtgtcagt ttgaaatcat
     7621 tccagagttc atccagcacc gtattacttg gtgtcagctt gctttgggtt tggtcatagt
     7681 aaccaacctc aacattagcg ccaaagcgct tttctccctt gataaacgga atttgatcca
     7741 cgattgattt gataaaggtt gattttccga taccattggg accaacgata gcgacagcat
     7801 tcatcttacg aagatctagg ttgataggtt gtgacaagac ttccccgtca taaccaatag
     7861 cagtattttc aacagtcaaa acaacattgc ccgacgtttt ttcagactgg aaggtcatgt
     7921 tggctgcttt cttgccagct tcaggcttgt ccaaacgctc cattttttct agttgtttac
     7981 ggcgagattg agcacgttta gtcgttgaag cacgaactag attgcgattg acaaagtctt
     8041 ccagagcagc gatttccttc tgttgctttt catagttttt tgcctcagta gctagctttt
     8101 gctccttcaa ttcgacaaaa cgagagtaat tccccacata gcgatccaag gaatgcttgg
     8161 tcaaatctag cgtaattgtc gcaaccttgt ccaagaaata acggtcgtgg ctgacgataa
     8221 tgagggcacc gctatagttt accaagtaat tctctagcca ggagatggtt tcaatatcca
     8281 agtggttagt tggctcgtcc aagaccaaga gattgggctt ttcaaggagc attttagcta
     8341 gagccaaacg agtattttga ccacctgaaa gttcagctat tttcatctgc cacatagact
     8401 cgtcaaactt gaatccattc aaaatcgctc gaatatcagc ttcataggta aagccgcctg
     8461 cttggcgaaa attctcagat aagtggtcgt aatctgacat cagtttatcc aaatcctcac
     8521 cagacttttc acccatctcc aactccatct gacgaagttg tttatctgtc cgacgtaagt
     8581 cattaaagac atgaagcatt tcatcgtaga tggtattttc agactcaaaa cggctatctt
     8641 gggctaggta agacagagaa atatcttttt tcttattgat ttctccgcta gttggctcct
     8701 cttctccaac taaaatcttc aaaagagtag acttacctgc accatttttc ccaacaaggg
     8761 caatccgatc tcgttcatca acctgcaggt tgatattatc gaaaagaacc tctcctgcaa
     8821 aagaacgttc aattttatta gcttgtaaaa taatcataag agtagtatag catgtttccc
     8881 t
//
LOCUS       DAVLTM010000078         8785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLTM010000078 DAVLTM010000000
VERSION     DAVLTM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.079"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            115..5418
                     /locus_tag="VYN16_001533"
     CDS             115..5418
                     /locus_tag="VYN16_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1404041.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPTDLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDLYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FESLDAPAQPGEGLENAFDGNVSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5584..7743)
                     /locus_tag="VYN16_001534"
     CDS             complement(5584..7743)
                     /locus_tag="VYN16_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEV1404042.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7740..8336)
                     /gene="recU"
                     /locus_tag="VYN16_001535"
     CDS             complement(7740..8336)
                     /gene="recU"
                     /locus_tag="VYN16_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1404043.1"
                     /translation="MVNYPHKVSSQKRQKSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8402..>8785
                     /locus_tag="VYN16_001536"
     CDS             8402..>8785
                     /locus_tag="VYN16_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HEV1404044.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTT"
BASE COUNT         2645 a         1675 c         2016 g         2449 t
ORIGIN      
        1 aaagcataat tattttttat ttatcagggt taagcattgc actttcagag gaaggagtat
       61 tttttaaaaa gaaaatgtaa acgcttgctc aaaaatgaaa ggatttagaa gtttatgaat
      121 aaaggattat ttgaaaaacg ttgtaaatat agtattcgga aattttcatt aggtgttgct
      181 tctgttatga ttggagctgc attctttggg acaagtccgg ttcttgcaga tagcgtgcag
      241 tctggttcca cggcgaactt accaactgat ttagctactg ctcttgcaac agcaaaagag
      301 aatgatgggc gtgattttga agcgcctaag gtgggagaag accaaggttc tccagaagtt
      361 acagatggac ctaagacaga agaagaacta ttagcacttg aaaaagaaaa accggctgaa
      421 gaaaaaccaa aagaggataa acctgcagct gctaaacctg aaacacctaa gacggtaacc
      481 cctgaatggc aaacggtaga gaaaaaagaa caacagggaa cagtcactat ccgagaagaa
      541 aaaggtgtcc gctacaacca actatcctca actgctcaaa atgataacgc aggcaaacca
      601 gccctgtttg aaaagaaggg cttgaccgtt gatgccaatg gaaatgcaac tgttgattta
      661 accttcaaag aagattctga aaagggcaaa tcacgctttg gtgtcttttt gaaatttaaa
      721 gataccaata ataatgtttt tgtcggttat gacaaggatg gctggttctg ggagtataaa
      781 tctccaacaa ctagcacttg gtatagaggt agtcgtgttg ctgctcctga aacaggatca
      841 acaaaccgtc tctctatcac tctcaagtca gacggtcagc taaatgccag caataacgat
      901 gtcaatctct ttgacacagt gactctacca gctgcggtca atgaccatct taaaaatgag
      961 aagaagattc ttctcaaggc gggctcttat gacgatgagc gaacagttgt tagcgttaaa
     1021 acggataacc aagagggggt aaaaacagag gatacccctg ctgaaaaaga aacaggtccg
     1081 gttgttgacg atagcaaggt gacttatgac acgattcagt ctaaggtcct caaagcagtg
     1141 attgaccaag ccttccctcg tgtcaaggaa tacagcttga atggacatac tttgccagga
     1201 caggtgcaac agttcaacca agtctttatc aataaccacc gaatcacccc tgaagtcact
     1261 tataagaaaa tcaatgagac aacagcagag tacttgatga agcttcgcga tgatgctcac
     1321 ttaatcaatg cggaaatgac agtacgcttg caagttgtgg acaatcaatt gcactttgat
     1381 gtgaccaaga ttgtcaacca caatcaagtc actccaggtc aaaagatcga tgacgaaaga
     1441 aaactacttt cttctattag tttcctcggc aatgctttag tctctgtttc tagtgatcaa
     1501 actggtgcta agtttgatgg ggcaaccatg tcaaacaata cgcatgtcag cggagatgat
     1561 catatcgatg taaccaatcc aatgaaagac ctagccaagg gttacatgta tggatttgtt
     1621 tctacagata agcttgctgc tggtgtttgg agtaactctc aaaacagcta tggtggtggt
     1681 tcgaatgact ggactcgttt gacagcttat aaagaaacag tcggaaatgc caactatgta
     1741 ggaatccaca gctctgaatg gcaatgggaa aaagcttata agggcattgt tttcccagaa
     1801 tacacgaagg aacttccaag ggctaaggtt gttatcactg aagatgccaa tgcagacaag
     1861 aaagtcgatt ggcaggatgg tgccattgct tatcgtagca ttatgaacaa ccctcaaggt
     1921 tgggaaaaag ttaaggatat cacagcttac cgtatcgcga tgaactttgg ttctcaagca
     1981 caaaacccat tccttatgac cttggatggt atcaagaaaa tcaatctcca cacagatggt
     2041 cttgggcaag gtgttctcct taaaggatat ggtagcgaag gccatgactc tggtcacttg
     2101 aactatgctg atattggtaa gcgtatcggt ggtgtcgaag acttcaagac cctaattgag
     2161 aaggctaaga aatatggagc tcatctaggt atccacgtta acgcttcaga aacttatcct
     2221 gagtctaaat acttcaatga aaaaattctc cgtaagaatc cagatggaag ctatagctat
     2281 ggttggaact ggctagatca aggtatcaac attgatgctg cctatgacct agctcatggt
     2341 cgtttggcac gttgggaaga tttgaagaaa aaacttggtg acggtctcga ctttatctat
     2401 gtggacgttt ggggtaatgg tcaatcaggt gataacggtg cctgggctac ccacgttctt
     2461 gctaaagaaa ttaacaaaca aggctggcgc tttgcgatcg agtggggcca tggtggtgag
     2521 tacgactcta ccttccatca ctgggcagct gacttgacct acggtggcta caccaataaa
     2581 ggtatcaaca gtgccatcac ccgctttatc cgtaaccacc aaaaagatgc ttgggttggg
     2641 gactacagaa gttatggtgg tgcagccaac tatccactgc taggtggcta cagcatgaaa
     2701 gactttgaag gctggcaagg aagaagtgac tacaatggct atgtaaccaa cttatttgcc
     2761 catgacgtca tgaccaagta tttccaacac ttcactgtaa gcaaatggga aaatggtaca
     2821 ccggtgacta tgaccgataa cggtagcacc tataaatgga ctccagaaat gcgagtggaa
     2881 ttggtagatg ctgacaataa taaagtagtt gtaactcgta agtcaaatga tgtcaatagt
     2941 ccacaatatc gcgaacgtac agtaacgctc aacggacgtg tcatccaaga tggttcagct
     3001 tacttgactc cttggaactg ggatgcaaat ggtaagaaac tttctactga taaggaaaag
     3061 atgtactact tcaatacgca ggccggtgca acaacttgga cccttccaag cgattgggca
     3121 aagagcaagg tttaccttta caagctaact gaccaaggta agacagaaga gcaagaacta
     3181 actgtaaaag atggtaaaat tactctagat cttctagcaa atcaaccata cgttctctat
     3241 cgttcgaaac aaaccaatcc tgaaatgtca tggagcgaag gcatgcacat ctatgaccaa
     3301 ggatttaaca gtggtacctt gaaacattgg accatttcag gcgatgcttc taaggcagaa
     3361 attgtcaagt ctcaaggggc aaacgatatg cttcgtattc aaggaaacaa agaaaaagtt
     3421 agtctcactc agaaattaac tggcttgaaa ccaaatacca agtatgccgt ttatgtcggt
     3481 gtcgataacc gtagtaatgc caaggcgagc gtcactgtaa atactggtga aaaagaagtg
     3541 actacttata ccaataagtc tctcgccctc aactatgtaa aagcctacgc ccacaataca
     3601 cgtcgtaaca atgctacagt tgacgataca agttacttcc aaaacatgta cgccttcttt
     3661 acaactggat cggacgtatc aaatgttact ctgacattga gtcgtgaagc tggtgatgaa
     3721 gcaacttact ttgatgaaat tcgtaccttt gaaaacaatt caagcatgta cggagacaag
     3781 catgatacag gtaaaggcac cttcaagcaa gactttgaaa atgttgctca gggtatcttc
     3841 ccatttgtag tgggtggtgt cgaaggtgtc gaagacaacc gcactcactt gtctgaaaaa
     3901 cacgatctat atacacaacg tggttggaat ggtaagaaag tcgatgatgt tatcgaagga
     3961 aattggtcac tcaagacaaa tggactagtg agccgtcgta acttggttta ccaaaccatc
     4021 ccacaaaact tccgttttga agcaggtaag acctaccgtg taacctttga atacgaagca
     4081 ggttcagaca atacctatgc ttttgtagtc ggtaagggag aattccagtc aggtcgtcgt
     4141 ggtactcaag caagcaactt ggaaatgcat gaattgccaa atacttggac agattctaag
     4201 aaagccaaga aggcaacctt cctcgtgaca ggtgcagaaa caggggatac ttgggtaggt
     4261 atctactcaa ctggaaatgc aagtaatact cgtggtgatt ctggtggaaa tgccaacttc
     4321 cgtggttata acgacttcat gatggataat cttcaaatcg aagaaattac cctaacaggt
     4381 aagatgttga cagaaaatgc tctgaagaac tacttgccaa cggttgccat gactaactac
     4441 accaaagagt ctatggatgc tttgaaagag gcggtcttta acctcagtca ggccgatgat
     4501 gatatcagtg tggaagaagc gcgtgcagag attgccaaga ttgaagcctt gaagaatgct
     4561 ttggttcaga agaaaacggc tttggtagca gaagactttg aaagtttgga tgcgccagct
     4621 caaccaggtg aaggcctaga gaatgctttt gatggcaatg tgtctagtct atggcataca
     4681 tcatggggcg gaggagatgt aggcaagcct gcaaccatgg tcttgaaaga agcaactgaa
     4741 atcactggac ttcgttatgt tccacgtgga tcaggttcaa atggtaactt gcgtgatgtg
     4801 aaacttgttg tgacagatga gtctggcaag gagcatacct ttactgcaac tgattggcca
     4861 gataacaata agccaaaaga cattgatttt ggtaagacaa ttaaggctaa gaaaattgtc
     4921 cttacaggta ctaagactta cggagatggt ggcgataaat accaatctgc agcggaactt
     4981 atctttactc gtccacaggt agcagaaaca cctcttgact tgtcaggcta tgaagcagct
     5041 ttggctaagg ctcagaaatt aacagacaaa gacaatcaag aggaagtagc tagcgttcag
     5101 gcaagcatga aatatgcgac ggataaccat ctcttgacgg aaagaatggt ggaatacttt
     5161 gcagattatc tcaaccaatt aaaagattct gctacgaaac cagatgctcc aactgtagag
     5221 aaacctgagt ttaaacttag ctctttagct tccgatcaag gtaagacgcc agattataag
     5281 caagaaatag ctagaccaga aacacctgaa caaatcttgc cagcaacagg tgagagtcaa
     5341 tctgacacag ccctcttcct agcaggtgtt agcctagccc tatctgctct ctttgtagta
     5401 aaaacgaaga aagactagta tttagtaaaa cctcttaaca agattacgga agcagtctct
     5461 atcttttcca atgaggttta tagtacagaa aaagtctgag aagatgtctt ctcagacttt
     5521 tgttaagcac ataaatacaa tagtgctatg acaaaatcac ccagaaaatc tgggtgataa
     5581 atgttatggt tgtgctggtt gaggattctg attttgttga tcaggggttg tatttgattg
     5641 ttgcgtatta ttgttaggat tggtagtcgt actattattt gtgcttggag tggttgagct
     5701 agactgtgaa gttgaactat ctgatgatga gcttgaactt tcagttgatg ggggttgttg
     5761 tggagcaggt gagctccacg tagaacgagc accattttta aatacgaatt ctccatttct
     5821 gtagagcccc tctggtatat tccaatcctc tggattgctt ccttcagaca ggtaggtcat
     5881 catagagcgg taaactttgg cagcgaccgt aaggccattg cctacaagtg gtgtcagacg
     5941 gttagaatag cctgtccata cagccattga atatttacgc gtatagccag caaatagttc
     6001 atcaggtgct acaaattgag aggtcttgat gtggttttca atttcctcgt ctgtatagtt
     6061 agaggttcct gttttaccag cctgagggag ccaagcaaga taggcatttc gtccagttcc
     6121 ataagtcaag actgttttca tcatgtcggt catcatatag gctgtcgttt ctttcatggc
     6181 acgagttccg acattagaga actctttttc actcccatca ctaaagacga ctttatggat
     6241 atacattggt ttatagtaag ttccaccatt tgcaaaggca gcgtaagcag cagccatctt
     6301 ttcactactt gctccatatt ttttgtctga ttcggttgtg ttacttgaaa tggcatttga
     6361 gtagtgaata cttgggtagt cgattcctag accatttagg aaagtcttgg cgcggttgag
     6421 tccgaccttg tttagagttt ccacggctgg gacgtttcgc gattgttgca gggcgtattg
     6481 caaggtgatg ttgccaaagt agcccctatc ccagttataa acaggagtat ttgtcccagg
     6541 gtagttatag ggctcatcgt gaacgatagt agcagttgaa tcgtagacac cgtactccaa
     6601 ggcaggagca tagtctgtga tcggtttcat agttgatccc cagtcgcggt ttgtttctac
     6661 tgcttggtta attccgaagg aaacattact tgactgatgg cgtgctccta gctgggcaat
     6721 gactttaccg ttagaaacat caacaatggt agaagcgact tgcaattcat cgtctggata
     6781 ggcaacgtat tcgtctgtat tgtaaatatc ccacagatgt ttttgagctt cttggtctac
     6841 atttgtgtag acatccatcc cagttgtgag caggttatag cctgtttctt cttcaacttg
     6901 attgatgact tccttgaggt aattatccat gtaagcaggg taattacttg ctgatttgag
     6961 actttgtagt ccatcagtaa ttggtgtatt gactgctttc tcatactgtt cagcagagat
     7021 gtagccttga tttttcattt cagataagac caagtttcgg cggtcttggg ctgcttctgg
     7081 atgtgaatag gggtcatatt ggtttggtgc ctgaggcatt ccagccagca aggctaactg
     7141 aggtaaactt aaattattga ggtctttacc atagtagttt tgagctgctg tctgcattcc
     7201 atagttccca ttagacatgt agaccttatt tatatagtag gtcaagattt cttgcttggt
     7261 tgctttttgt tctaactgaa tcgctaacca agcttcctga gccttacgag aaatagtctg
     7321 gtcggaagtc gaagttgaaa agtaagtcaa cttaatcaac tgttgagtga gagttgatcc
     7381 accttggagg gaattgcttt gcagattgcg caagaaagct cccaggatac ggatggtatc
     7441 aatccccctg tggtcgaaga agcgatggtc ttcgatagaa acgattgcct taaccaaatc
     7501 tgtgggaata tcattagctt gggcattgac gcggcgttca gaacccaagt cagcaatgag
     7561 ttgattttta ttgtcgtaga ttttactaga agttgttgca actagtttac tctcggatag
     7621 gctaggagcc ttgctaacgt agtagaaaaa aactcctccg cctaagacaa tggctgcgat
     7681 aaccaagctt aagaagctaa tgctcagata cttgattagg cgcagaatcg ttggtttgtt
     7741 catcttgttt taccacctaa taaatgttct ttgataacat tgagataagg aatttgagga
     7801 aaggcaccag ccttgatttc atatccatat tctcgaatat attcaagtgg cattgatttt
     7861 tgtcccttat cttgatgata gaagcgaatc aaatcgaatg ccggcaataa gtaggtttct
     7921 tgctgagaag aaaagtgaag aaggacaaag cagattcctt gttgggcaag gacttgttcc
     7981 atatgctgaa tctgatgtgg atgaaaattt ttcatcggaa tcgcacgttt ttgttttgtt
     8041 tccttgactt caaagtcgat gtaatatcca ttataaacgc cagaatagtc cgtcgttgaa
     8101 gcttgtcgaa aataggcttc aacaatcttg gcacgacttc gttgtggata gtccacttgt
     8161 acgatttgaa taggagttgg tttcttatgt atgacagcca agccctgaga caaatagtag
     8221 tcgttggtag cattgatcat cttttcaaaa gacattcctc gatttgcgaa atttttgggt
     8281 tgagaaagag atttttgtct tttttgtgat gaaactttat gtggatagtt gaccataatt
     8341 ctccttattg gtacaataac atcactttat tatatcataa tttgcacaga aagggttaaa
     8401 aatggctaca gctttggttt taggctattc tgcttttgat ttgggtttat tttctgataa
     8461 ggatcctcgt cttaaattga ttaaaaaagc gatacgtaaa gatttagagg ctatggcagc
     8521 agatggggtg tcttggcttg tgtttacagg aagtttgggt tttgaatatt gggttttaga
     8581 ggttgctcag gagatgaaaa ccgaatacgg tttccagttg gccaccattt ttgcttttga
     8641 aacccatggg gaaaattgga atgaaggcaa tcagatgaaa ctgagtcgct ttaagcaggt
     8701 agactttgtc aaatatgctt atcctcgcta tgaacacaag gggcagttaa gagattacca
     8761 gcagtttttg ctggaaaaca cgact
//
LOCUS       DAVLTM010000079         8645 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLTM010000079 DAVLTM010000000
VERSION     DAVLTM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.080"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            11..202
                     /gene="secG"
                     /locus_tag="VYN16_001537"
     CDS             11..202
                     /gene="secG"
                     /locus_tag="VYN16_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1404045.1"
                     /translation="MIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEAVMQRLTG
                     ILVFFWLAIALALTVLSSR"
     gene            304..2658
                     /gene="rnr"
                     /locus_tag="VYN16_001538"
     CDS             304..2658
                     /gene="rnr"
                     /locus_tag="VYN16_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1404046.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERIIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLCIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2621..3088
                     /gene="smpB"
                     /locus_tag="VYN16_001539"
     CDS             2621..3088
                     /gene="smpB"
                     /locus_tag="VYN16_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1404047.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3104..3964
                     /gene="tehB"
                     /locus_tag="VYN16_001540"
     CDS             3104..3964
                     /gene="tehB"
                     /locus_tag="VYN16_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1404048.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4037..4990
                     /gene="coiA"
                     /locus_tag="VYN16_001541"
     CDS             4037..4990
                     /gene="coiA"
                     /locus_tag="VYN16_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1404049.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQYFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5009..6811
                     /gene="pepF"
                     /locus_tag="VYN16_001542"
     CDS             5009..6811
                     /gene="pepF"
                     /locus_tag="VYN16_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1404050.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKIGLA"
     gene            6813..7526
                     /locus_tag="VYN16_001543"
     CDS             6813..7526
                     /locus_tag="VYN16_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1404051.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7593..8534
                     /gene="prsA"
                     /locus_tag="VYN16_001544"
     CDS             7593..8534
                     /gene="prsA"
                     /locus_tag="VYN16_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1404052.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT         2788 a         1601 c         1917 g         2339 t
ORIGIN      
        1 attatctgtt gtgattgtga ttgcaatttt catgcaacca accaaaaacc aatccagcaa
       61 tgtatttgat gccagttcag gtgatttgtt tgaacgcagt aaagctcgcg gttttgaagc
      121 tgtaatgcag cgtttgacag ggattttagt ctttttctgg ctagccattg ccttagcatt
      181 gacggtatta tcaagtagat aagaaaataa tgggcaggac taggtctttg cctcttttta
      241 tttttaaagg atgtttgaga aggttttaca gtaaaagaaa attaaaaaat ctagaaagaa
      301 aatatgaaag atagaataaa agaatattta caagacaagg gaaaggtgac tgttaatgat
      361 ttggctcagg ctttgggaaa agacagttcc aaggattttc gtgagttgat taaaaccttg
      421 tccttaatgg aaagaaagca ccagattcgc tttgaagaag atggcagtct gaccttggaa
      481 gccaagaaaa aacatgagat taccctcaag gggatttttc atgcccataa aaatggtttt
      541 ggttttgtca gcctagaagg ggaagaggac gacctttttg tagggaaaaa tgatgtcaac
      601 tatgctattg atggtgatac cgtcgaggta gtgattaaga aagtcgctga ccgcaataag
      661 ggaacagcag cagaagccaa aattattgat atcctagaac acagtttgac aacagttgtc
      721 gggcaaatcg ttctggatca ggaaaagcct aagtatgctg gctatattcg ttcaaaaaat
      781 cagaaaatca gtcaaccgat ttatgttaag aaaccagccc taaagttaga aggaacagaa
      841 gttctcaagg tctttatcga taaataccca agcaagaaac atgatttctt tgtcgcgagt
      901 gttctcgatg tggtgggaca ctcaacggat gtcggaattg atgttcttga ggttttggaa
      961 tcaatggaca ttgtatccga gtttccagaa gctgttgtta aggaagcaga aagtgtgcct
     1021 gatgctccgt ctcaaaagga tatggaaggt cgtctggatc taagagatga aattaccttt
     1081 accattgacg gtgcggatgc caaggacttg gacgatgcag tgcatatcaa ggctctgaaa
     1141 aatggcaatc tggagcttgg ggttcacatc gcagatgttt cttattatgt gaccgagggg
     1201 tctgcccttg acaaggaagc ccttaaccgt gcgacttctg tttacgtgac agaccgagtg
     1261 gtgccaatgc ttccagaacg actatcaaat ggcatctgct ctctcaatcc ccaagttgac
     1321 cgcctgaccc agtctgctat tatggagatt gataaacatg gtcgtgtggt caactatacc
     1381 attacacaaa cagttatcaa gaccagtttt cgtatgacct atagcgatgt caatgatatc
     1441 ctagctggcg atgaagaaaa gagaaaaaaa tatcataaaa ttgtaccaag tatcgaactc
     1501 atggccaagc ttcatgaaac tttagaaaac atgcgtgtga aacgtggagc tctcaatttt
     1561 gataccaatg aagcgaagat tttagtggat aaacaaggta agcctgttga tatcgttctt
     1621 cggcagcgtg gtattgccga gcggattatt gagtctttta tgttgatggc taatgaaaca
     1681 gttgccgaac atttcagcaa gttggatttg ccttttatct atcgaattca cgaggagcct
     1741 aaggctgaaa aggttcagaa gtttattgat tatgcttcga gttttggctt gtgcatttat
     1801 gggactgcca gtgagattag tcaggaggca cttcaagaca tcatgcgtgc tgttgaggga
     1861 gaaccttatg cagatgtatt gtccatgatg cttcttcgct ctatgcagca ggctcgttat
     1921 tcggagcaca atcacggcca ctatggacta gctgctgact attatactca ctttaccagt
     1981 ccaattcgtc gttatccaga ccttcttgtt caccgtatga ttcgagatta cggctgttct
     2041 aaggaaatag cagagcattt tgaacaagtg attccagaga ttgcgaccca gtcttccaac
     2101 cgtgaacgtc gtgccataga agctgagcgt gaagtcgaag ccatgaaaaa ggctgagtat
     2161 atggaagaat acgtgggtga agagtatgat gcagttgtat caagtattgt caaattcggt
     2221 ctctttgtcg aattgccaaa cacagttgaa ggcttgattc acatcactaa tctacctgaa
     2281 ttttatcatt tcaatgagcg tgatttgact cttcgtggag aaaaatcagg tatcactttc
     2341 cgagtgggtc agcagatccg tatccgtgtt gaaagagcgg ataaaatgac tggagagatt
     2401 gatttttcat tcgtacctag tgagtttgat gtgattgaaa aaggcttgaa acagtctagt
     2461 cgtagtggca gagggcgtgg ttcaaatcgt cgttcggata agaaggaaga caagagaaaa
     2521 ttaggacgct caaatgataa gcgtaagcat tcacaaaaag acaagaagaa aaaaggaaag
     2581 aaaccttttt acaaggaagt agctaagaaa ggagccaagc atggcaaagg gcgagggaaa
     2641 ggtcgtcgca caaaataaaa aggcacgcca cgactataca atcgtagata cgctagaggc
     2701 agggatggtc ctgactggaa ctgaaatcaa gagtgtacga gctgctcgaa ttaatctcaa
     2761 ggatggcttt gctcaagtga aaaatggaga agtttggctg agcaatgttc atatcgcgcc
     2821 ttacgaagag ggcaatatct ggaaccagga accagaacgt cgtcgtaaac tcctgctcca
     2881 taaaaagcaa attcaaaaat tggaacaaga gaccaaaggg acaggaatga ccttagttcc
     2941 ccttaaggtc tatataaaag atggctacgc taagcttctt ttaggacttg ccaaagggaa
     3001 gcatgactat gacaaacggg agtctatcaa acgtcgtgag caaaatcgag atatcgcgcg
     3061 tgtgatgaaa gctgttaatc agcgataaaa agaggaattg aaaatggaaa aattagttgc
     3121 ctataaacgc atgcctttgt ggaataaaca aacaatgcct gaagctgttc agcaaaagca
     3181 caatacaaaa gttgggactt gggggaaaat tactgtcttg aagggagctc tcaagtttat
     3241 tgaattgaca gaagaagggg aagttctagc tgaacacctc tttgaagcag gggcagacaa
     3301 tccaatggcc caacctcaag cctggcaccg agtggaagct gccacagatg atgtggaatg
     3361 gtacttggaa ttttattgta aacctgagga ttattttgct aaaaaataca ataccaatcc
     3421 tgttcattca gaggtcctag aggccatgca gacagtgaaa caagggaaag ctttggattt
     3481 gggttgtggt caggggcgta attctctttt tctagcccag caagattttg atgtgacggc
     3541 tgtagatcaa aatggactgg ctcttgaaat cttgcaaagc attgtggagc aggaagattt
     3601 ggacatgcct gttggccttt acgatatcaa ttcagctagc attggacaag aatatgattt
     3661 tatcgtttca acagttgttc tcatgtttct acaagcggac cgcattccag ctattattca
     3721 aaatatgcag gagaaaacca gtgttggtgg ttacaacctt atcgtttgtg ccatggacac
     3781 ggaggattat ccttgctcgg ttaatttccc attcaccttt aaagaaggag aactggcaga
     3841 ctattacaag gattgggaat tggttaagta caatgaaaat ccaggccatt tgcatcgtcg
     3901 cgatgagaat ggcaatcgta ttcaactacg ctttgcgacc ttactagcta agaaaatcaa
     3961 gtaaacacac atgaagatta ggaattttcc tgatcttttt tcttttttac gaatgatata
     4021 gaaaaggagg gaattcatgt ttgttgcgag agatgctagg ggagaattgg taaatgtgtt
     4081 agaggataaa cttgagaagc aagcatacac ctgcccagct tgtggaggcc agctccattt
     4141 gcgtcaagga ccaagtgtac ggacgcattt tgcccataaa tccttaaaag actgtgattt
     4201 tttctttgaa aatgaaagtc cagaacacct ggccaataag gaatccctct atcactggtt
     4261 gaaaaaagag acaaaggttc aattagagta cccgctttca gaacttaaac agattgcgga
     4321 tgtatttgta aatggcaatc tagctctaga agttcagtgt agtcccttgc ctcagaaagt
     4381 ccttaaagag cgaagtgagg gatatcgtag tcagggttac caagtactgt ggttgctggg
     4441 tcaaaaactg tggctcaagg agcgtttgac tcgtctacag caaggttttc tttatttcag
     4501 tcaaaacatg ggcttttatg tttgggaatt agacaaggaa aaacaagttt taagactcaa
     4561 atacctgatt taccaggatc tccgcggtaa actccattat caaatcaagg aattttccta
     4621 tggtcaaggt agtttattgg aaatattgcg tcttccctat aagagacaaa aaatatctca
     4681 ttttacagtt tctgaggaca aggacatctg tcgctatatc cggcaacaac tttattatca
     4741 aaatctcttt tggatgaaag aacaagcaga agcctatcaa aagggagaaa atatcctgac
     4801 ttatggactg aaagaatggt atccacaaat tcgaccaata gtgggcaaat ttttccagat
     4861 tgaacaagac ttgactagct attatcagta cttttatacc tattaccaaa aaaatcctca
     4921 aaatgattgg caaaagcttt atccaccagc cttttatcag caatatttct tgaaaaatat
     4981 ggtagaataa aaaggatgga ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa
     5041 agatacatgg gatctatcaa cgatctaccc aactgaccag gcttgggaag aagccttaaa
     5101 agatttaaca gaacaattgg agacagtagc ccagtatgaa ggccatctct tggatagtgc
     5161 ggataaccta ctagaaatca ctgaattttc tcttgaaatg gaacgccaga tggagaagct
     5221 ttacgtttat gctcatatga agaatgacca ggatacacgt gaagctaagt atcaagagta
     5281 ctatgccaag gccatgacac tctacagcca gttagaccaa gccttttcat tctatgatcc
     5341 tgaatttatg gagattagcg aaaagcagta tgctgacttt ttagaagctc aaccaaagct
     5401 gcaggtttat caacactatt ttgacaagct cttgcaaggc aaggatcacg ttctttcaca
     5461 acgtgaagaa gaattattgg ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt
     5521 cgctatcttg gacaatgcgg atattgtgtt cccttatgtc ctagacgatg atggtaaaga
     5581 agttcagcta tctcatggga cttacacacg tttgatggag tctaaaaaac gtgaggttcg
     5641 ccgtggtgcc tatcaagctc tttatgcgac ttacgaacaa ttccaacaca cctatgccaa
     5701 aaccttgcaa accaatgtta aggtgcaaaa ttaccgtgct aaagttcgta actacaagag
     5761 tgctcgtcat gcagccctcg cagcgaattt tgttccagaa agtgtttatg acaatttggt
     5821 agcagcagtt cgcaagcatt tgccactctt acatcgctat cttgagcttc gttcaaaaat
     5881 cttggggatt tcagatctca agatgtacga tgtctacaca ccgctttcat ctgttgaata
     5941 caattttacc taccaagaag ccttgaaaaa agcagaagat gctttggcag tcttgggtga
     6001 ggattacttg agccgtgtca aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa
     6061 tcaaggcaag cgttcaggtg cctactctgg tggttcttac gataccaatg cctttatgct
     6121 tctcaactgg caggacaatc tggacaatct ctttactctt gttcatgaaa caggtcacag
     6181 tatgcattca agctatactc gtgaaactca gccttatgtt tacggagatt actctatctt
     6241 tttggctgag attgcctcaa ctaccaatga aaatatcttg acggagaaat tattggaaga
     6301 agtggaagac gacgcaacac gctttgctat tctcaataac ttcctagatg gtttccgtgg
     6361 aacagttttc cgccaaactc aatttgctga gtttgaacac gccattcacc aagcagatca
     6421 aaatggggag gtcttgacaa gcgatttcct aaataaactc tacgcagact tgaaccaaga
     6481 gtattatggt ttgagtaagg aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc
     6541 acacttctac tataactact atgtatatca atattcaact ggctttgcgg ccgcctcagc
     6601 cttggctgaa aaaattgtcc atggtagtca agaagaccgt gaccgctata tcgactacct
     6661 caaggcaggt aagtcggact atccacttaa tgtcatgaga aaagctggtg ttgatatgga
     6721 gaaggaagac tacctcaacg atgcctttgc agtctttgaa cgccgtttaa atgagtttga
     6781 agcccttgtt gaaaaaatag gattggcata aaatggttga atcgtatagt aagaatgcta
     6841 accataacat gcgtcgtcct gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc
     6901 aaaagcaagt cacaggttcc ttgaaagaat tggaagactt tgcccgcaag gaaaatattc
     6961 ctattattcc ccatgaaacg gttgcttatt tccgttttct tatggaaacc atgcagccta
     7021 aaaatattct ggaaattggg acggctatcg gtttttcagc tctcttgatg gctgaacatg
     7081 cgccaaatgc taagattaca actattgatc gtaatccaga aatgattggt tttgccaagg
     7141 aaaattttgc ccagtttgac agtcgcaagc aaatcactct cctagaggga gatgcggtgg
     7201 atgtcttatc tacactgaca gagtcttatg atttcgtctt tatggattct gccaagtcta
     7261 aatacatcgt ctttctgcca gaaatcctca aacatttgga agttggtggt gtggttgtct
     7321 tggatgatat ttttcaaggt ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc
     7381 agcgaaccat ttatcgaggc cttcaaaaat tatttgatgc aaccttagac aatccagaac
     7441 tcaccgcaac attagtgcct ttaggagatg gtattctcat gcttcgtaaa aatgtagcag
     7501 atgttcaact gtctgaaagc gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata
     7561 gatagagtaa cacttatctc aaaggagtag acatgaagaa aaaattattg gcaggtgcca
     7621 tcacactatt atcagtagca actttagcag cttgttcgaa agggtcagaa ggggcagacc
     7681 ttatcagcat gaaaggggat gtcatcacag aacatcaatt ttatgagcaa gtgaaaagca
     7741 acccttcagc ccaacaagtc ttgttaaata tgaccatcca aaaagttttt gaaaaacaat
     7801 atggctcaga gcttgatgat aaagaggttg atgatactat tgccgaagaa aaaaaacaat
     7861 atggcgaaaa ctaccaacgt gtcttgtcac aagcaggtat gactcttgaa acacgtaaag
     7921 ctcaaattcg tacaagtaaa ttagttgagt tggcagttaa gaaggtagca gaagctgaat
     7981 tgacagatga agcctataag aaagcctttg atgagtacac tccagatgta acggctcaaa
     8041 tcatccgtct taataatgaa gataaggcca aagaagttct cgaaaaagcc aaggcagaag
     8101 gtgctgattt tgctcaatta gccaaagata attcaactga tgaaaaaaca aaagaaaatg
     8161 gtggagaaat tacctttgat tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg
     8221 ctttcgcttt agatgtggat ggtgtttctg atgtgattac agcaactggc acacaagcct
     8281 acagtagcca atattacatt gtaaaactca ctaagaaaac agaaaaatca tctaatattg
     8341 atgactacaa agaaaaatta aaaactgtta tcttgactca aaaacaaaat gattcaacat
     8401 ttgttcaaag cattatcgga aaagaattgc aagcagccaa tatcaaggtt aaggaccaag
     8461 ccttccaaaa tatctttacc caatatatcg gtggtggaga ttcaagctca agcagtagta
     8521 catcaaacga atagtccaaa tcaatgagtc agggaaaaaa ctcgacttca ggaaaaaatg
     8581 aagcaaacat tcccacaata aaacgcatag tacaaggttt gtactgcccc ccaaaaaagt
     8641 tagac
//
LOCUS       DAVLTM010000080         8568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLTM010000080 DAVLTM010000000
VERSION     DAVLTM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.081"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            152..451
                     /locus_tag="VYN16_001545"
     CDS             152..451
                     /locus_tag="VYN16_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1404053.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWNGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            522..2543
                     /locus_tag="VYN16_001546"
     CDS             522..2543
                     /locus_tag="VYN16_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1404054.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFIYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDIENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNILYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2545..3186
                     /locus_tag="VYN16_001547"
     CDS             2545..3186
                     /locus_tag="VYN16_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404055.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3290..4642
                     /locus_tag="VYN16_001548"
     CDS             3290..4642
                     /locus_tag="VYN16_001548"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1404056.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVIPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELAIQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4644..5702
                     /locus_tag="VYN16_001549"
     CDS             4644..5702
                     /locus_tag="VYN16_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1404057.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5712..6878
                     /gene="divIB"
                     /locus_tag="VYN16_001550"
     CDS             5712..6878
                     /gene="divIB"
                     /locus_tag="VYN16_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1404058.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFLSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKATIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESN"
     gene            7144..7845
                     /gene="pyrF"
                     /locus_tag="VYN16_001551"
     CDS             7144..7845
                     /gene="pyrF"
                     /locus_tag="VYN16_001551"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1404059.1"
                     /translation="MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            7879..8511
                     /locus_tag="VYN16_001552"
     CDS             7879..8511
                     /locus_tag="VYN16_001552"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1404060.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQKNWQEG"
BASE COUNT         2631 a         1561 c         1919 g         2457 t
ORIGIN      
        1 aaatatctaa aaaattcgat tttttccttg acaacaaata ataaaaatgt tattcttatc
       61 acataagaaa gcggtttcaa caaaagagag aggaggattt attttgttgc attgttgctc
      121 agggtgtgct ttttttgaaa gaaaaaataa aatgaaaacg aaaaaacata gattacttgc
      181 tctagctctt atttcaagtt ttacattatt gggagctgca tcagctgctg tacaatatcc
      241 agatggagga gtatggacat atggagaagg ttcaggaggt ggttgggctt tttcaaatta
      301 ctatcatggt aaaaaatatc attattcatc catcgtgagc agatggaatg gtcattctga
      361 taaaggagaa gctcctgctg gaaaaacctc ctatgcatgg atttggacta aatggggaga
      421 acaagtagca ttttactgtg attatgacta atcgttcatt gatttaaatc gttccccttg
      481 taagaaatta cagggggaat tttgtttaca aggagtctac tatgaagaaa ctattcatat
      541 tattatcaac tttttttctc agcttcttcc ttgcttggat tattgtctta cgtgcgccac
      601 aatatttata tgcaagctat gattccgttt ctttacttcg tgtcaaaaaa gatactcagg
      661 aaccgacgcg tgaggtattt gaacaggaat tggagaattt tgcaaattca gaacagagtt
      721 taatagctag aagaattgta gagccgagta aggatgggac gactcacttt atttatgcaa
      781 cttatggtca gggaacttta ccaaaagaat tccaagaagc tagccaagaa agtcgtgaac
      841 gtagtgatcc gctaaatagt tatctccttt tgtcaggttc cttgacgaaa gaaaagcttg
      901 ccgataaatt aggagatttg ggttataaag caagtgctga ccgaaagata ccgccctatt
      961 ttcttgcttt tcgaatatta ctaaatcccc ttattttaat tagtttagca atatttggct
     1021 tatctttctt tgctttagtg attatcactc ggattaagga aatgagagca gcaggtataa
     1081 aactcttttc tggtcagact ctcttatcca tcatggggca ttctttatct actgatatca
     1141 aatggctcct tctatcagcc ctcctttcct tcctaggtgg gggagtcgtt ctttttagtc
     1201 aaggtttgtt ttatcctatc ttgttagcca cctatggttt tgggattagt ttctatctgt
     1261 tgtttttatt ggcgatttca attttactaa tgctccttta tctaatgagt ttgaattaca
     1321 aagcattagt tcccgttatt aaggggagat taccccttaa acgcctgatg attttaaccc
     1381 tattgtgtca gttggttgct gtctttacag taggatacgc tgttaagaca ggtttgacgt
     1441 cttaccaacg attgaaagaa cttgaaattt caaaacaagc atggcaggat agagcagact
     1501 attatcaaat ttcttttggc ttaggtgata gaggaaaaga tatagaaaat cagagcaagt
     1561 ggtatgcctt tgccaaggaa gcaatcgaag aagaacaagc tctttatgta aaggataatc
     1621 tgctccattt tgccaatcca caaggaaaaa atgaacaggg agagacactg gatacctata
     1681 gtccagatgc taatacgctc tatgttagtc ccagttattt ggacaaggaa aaggtcgtgg
     1741 tagatgctga gaccaaacag aagttagccc atctccaaaa aggtgagttt atcctcttgc
     1801 tcccagaaca tttgcgctct cgagaagcag aacttaagaa agtttttgaa gaaagattga
     1861 gttattatgg aaaatctggt gaggaggcaa gtgctccttt ggattatgag atgaaagcgc
     1921 acgttagtta tctttcaatg ggagaaaagc ggtttgttta taataacggt gagaatcccg
     1981 tatccactca gtatttgact gatccgattt tagtggtatt cacgccgact tctacaggtg
     2041 atagttttat atccttatct agttggtcta tcaatgctgg aaaacaactc tttatcaaag
     2101 gatatgagag tgggctagaa ctcttgaaga aagctggaat ttatgagcaa gtatcctatc
     2161 ttaaagaagg aagaagtgtt tatctaactc gttataatga agttcaaact gaaacagcaa
     2221 ctttaatctt aggagctatt gtggggatag ctagttcctt gttactattt tattctgtca
     2281 atattctata tttcgagcaa ttccgccgag atatcttgat taaacgaatt tcaggtttac
     2341 gattttttga aacacatgct cagtatatgg ttagccaatt tgccagtttt gtatttggtg
     2401 ctagtctctt tattttaagc agtcgagact tggtgattgg cttgctcact ttattggtct
     2461 ttctagctag tgcagttttg acgctttacc gtcaagcgca gaaagaatct cgtgtttcta
     2521 tgacaattat gaaaggaaaa taggatgatt gaactaaaga atatatctaa aaaatttgga
     2581 agccgtcagc tattttcaga tacgaatctt cattttgaag gtgggaaaat ttatgcctta
     2641 atcggtacaa gtggctgtgg taagacaaca ctcttgaata tgattggacg attggagcca
     2701 tatgacaaag ggcaaatcat ctatgatggc acttctctta aggacatcaa gccttctgtt
     2761 ttctttagag attacttggg atacttattt caagattttg gcttaattga aagccaaacc
     2821 gtcaaagaga atctcaatct gggtttagtt ggtaaaaagt tgaaggaaaa agagaaaatc
     2881 tctttgatga aacaagctct aaaccgtgta aacctctctt atttggattt gaagcaacct
     2941 atatttgagt tatcaggagg agaagcacaa cgtgttgcac tagcgaagat aattttaaag
     3001 gatccgcctt tgattcttgc agatgaacca accgcttcct tagaccccaa aaactctgag
     3061 gaattacttt ccatcctaga atctttaaaa aatccgaatc ggaccattat tattgcgacc
     3121 cacaatcctc tgatttggga gcaagtggat caggtcattc gagttaccga tttatcacat
     3181 agatgatatg gtaagattca gttagaagaa agagtcacaa acacactttg tggctttttt
     3241 atttccataa aaatggtaaa atagtaggag tagaaatgga gttcgagaca tgaaagtaat
     3301 agatcaattt aaaaataaga aagttcttgt tttaggtttg gccaagtctg gtgaatctgc
     3361 ggctcgtttg ttggacaagt taggtgccat tgtgacagta aatgatggga aaccattcga
     3421 ggacaatcca gctgcccaat gtttgctgga agaagggatc aaggtcatta caggtggcca
     3481 tcctttggaa ctcttggatg aagagtttgc cctcatggta aaaaatccag gtatccccta
     3541 cagcaatccc atgattgaaa aggctttggc caagggaatt ccagtcttga ctgaggtgga
     3601 attggcttat ttgatttcag aagcaccgat tattggtatc acaggatcga acggtaagac
     3661 aaccacaacg actatgattg gggaagtttt gactgctgct gggcaacatg gtcttttatc
     3721 agggaatatc ggctatcctg ccagtcaggt tgctcaaata gcatcagata aggacacgct
     3781 tgttatggaa ctttcttctt tccaactcat gggtgttcaa gaattccatc cagagattgc
     3841 ggttattacc aacctcatgc caactcatat cgactaccat gggtcatttt cggaatatgt
     3901 agcagccaag tggaatatcc agaacaagat gacagcagct gatttccttg tcttgaactt
     3961 taatcaagac ttggcaaaag acttgacttc caagacagaa gccactgtta taccattttc
     4021 aacacttgaa aaggttgatg gagcttatct ggaagatggt caactctact tccgtggtga
     4081 agtagtcatg gcagcgaatg aaatcggtgt tccaggtagc cacaatgtgg aaaatgccct
     4141 tgcgactatt gctgtagcca agcttcgtga tgtggacaat caaaccatca aggaaactct
     4201 ttcagccttc ggtggtgtca aacaccgtct ccagtttgtg gatgacatca agggtgttaa
     4261 attctataac gacagtaaat caactaatat cttggctact caaaaagcct tgtcaggatt
     4321 tgacaacagc aaggtcgtct tgattgcagg tggtttggac cgtggcaatg agtttgacga
     4381 attggtgcca gacattactg gactcaagaa gatggtcatc ctgggtcaat ctgcagaacg
     4441 tgtcaaacgg gcagcagaca aggctggtgt cgcttatgtg gaggcgacag atattgcaga
     4501 tgcgacccgc aaggcctatg agcttgcgat tcaaggagat gtggttcttc ttagtcctgc
     4561 caatgctagc tgggatatgt atgctaactt tgaagtacgt ggcgacctct ttatcgacac
     4621 agtagcggag ttaaaagaat aatatgaaaa aaattgtctt tacaggtggg gggacggttg
     4681 gacacgttac cctcaatctt ttgttaatgc ccaagttcat cgaagatggt tgggaagtcc
     4741 actatatcgg ggacaagcgt ggtatcgaac accaagaaat ccttaagtca ggtttggatg
     4801 tcaccttcca ttctattgcg actggaaaat tgcgtcgcta tttctcttgg caaaatatgc
     4861 tggacgtctt caaagttggt tggggaattg tccaatcgct ctttatcatg ttgcgactgc
     4921 gtccacagac ccttttttca aaggggggct ttgtctcagt accgcctgtt atcgctgcgc
     4981 gtgtgtcagg agtgcctgtc tttattcacg aatctgacct gtctatgggc ttggccaata
     5041 aaatcgccta taaatttgcg actaagatgt attcaacctt tgaacaagct tcgagtttgg
     5101 ctaaggttga gcatgtggga gcggtgacca aggtttcgga caaaaacact ccagaaccag
     5161 atgaattggt ggatattcaa acccacttta atcccaaatt gccaactgta ttgtttgttg
     5221 gcggttctgc aggcgctcgt gtctttaacc aattggtgac agaccataag aaagaactaa
     5281 cagagcgcta caatattatc aatctaactg gagattctag cctgaatgag ttgaggcaaa
     5341 atctttttcg tgttgactat gtgaccgatc tctatcaacc cttgatggaa ttggctgata
     5401 ttgttgtgac acgaggtggt gccaatacga tttttgagct cttggcgata gcaaaattgc
     5461 atgtcattgt gccgcttggt cgtgaagcta gtcgtggtga ccagattgaa aatgcagctt
     5521 actttgtaaa aaaaggctat gcagaagacc ttcaagaaag cgatttgacc ttggatagtt
     5581 tggaagagaa gctttctcac ttactaagtc acaaggaaga ttaccaagct aagatgaaag
     5641 cttctaagga attgaaatct ctagcagatt tttatcaatt gttgaaaaaa gatttatcat
     5701 aaggaaagta aatgtcaaaa gataagaaaa atgaggacaa agaaatcctc gaagaattga
     5761 aagagttatc agaatggcag aaacgaaacc aagaatatct aaaaaagaag gctgaagaag
     5821 aagcggctct agctgaggag aaggaaaagg aaagacaagc tcggatggga gaagaatctg
     5881 agaagtcaga ggacaaacag gaccaggaga gtgaaacaga ccaggaagat tcagaatcag
     5941 ctaaggaaga gtctgaagaa aaagtagcat cctcagaggc tgacaaagag aaagaagaga
     6001 aagaagaatc agagtctaaa gagaaggagg aacaggataa aaagcttgct aaaaaggcta
     6061 caaaggaaaa accagccaaa gcaaagattc ctggtatcca tatcttgcga gccttcacga
     6121 ttttatttct aagtctgctt ttattgattg tctctgccta cttgctcagt ccttatgcga
     6181 ccatgaaaga tattcgtgtt gagggaacgg tgcaaactac agctgatgat attcgacagg
     6241 cttcaggcat tcaggattcg gattatacga ttaaccttct gctagacaag gcaaaatatg
     6301 aaaagcagat taagtctaac tattgggttg aatcagctca acttgtctat caatttccaa
     6361 ctaagttcac tattaaggtc aaggaatatg atattgtggc ctactatatt tctggtgaaa
     6421 atcattatcc tattctttcc agtggtcagc ttgagactag ttctgtgagt ctgaacagtt
     6481 taccagaaac ttatttatca gttctcttta atgatagtga acaaatcaag gtttttgtct
     6541 cagaacttgc tcaaattagc ccagaactca aggcgactat ccaaaaggtg gaattagccc
     6601 caagcaaggt gacatccgat ttaattcgat tgaccatgaa tgattcggac gaagtcttgg
     6661 ttcctctatc tgaaatgagt aagaaactgc catattacag taagattaag cctcaattgt
     6721 cagaaccgag tgtggtcgac atggaagctg gaatctacag ctacactgtg gcggataaat
     6781 taattatgga agctgaggaa aaagccaaac aagaggccaa ggaagccgag aaaaaacagg
     6841 aagaagaaca gaaaaaacaa gaggaagaga gcaattgaaa tcaaacgacc cagcgctcat
     6901 tgcgtcgcta ggtttacctt ttctcttata gttctttagt gaccatgttt ttacttgaca
     6961 agtgctagta gaaataatag aataatagaa aacctttaaa gcagtccaga gaggcagcta
     7021 aggttagacg gtgaaagggt ggagactacc catttttcgt ggaaccttgc tgttggcagg
     7081 ttcctttttt cgtggcttct gttggccaga ctctctcact agtaaaggta aaaggagaaa
     7141 cctatgcgag aacatcgtcc aatcattgct cttgattttc ctagttttga ggtggtcaag
     7201 gaatttttag ctcttttccc agcagaagaa agcctttatc tcaaggtagg gatggagctt
     7261 tattacgcag cggggcctga gattgtgtcc tacttaaaag gtttgggtca tagtgtcttt
     7321 ttggatctca aacttcatga cattcccaat acagtcaagt cagcaatgaa gatcctatct
     7381 cagcttggtg tggatatgac caatgttcat gcggctggtg gtgtagagat gatgaaggct
     7441 gcgcgtgaag gtcttggaag tcaagctaaa ttgatcgctg taactcagct cacatcaacg
     7501 tcagaagctc agatgcagga gtttcaaaat atccaaacca gtctgcaaga gtctgtgatt
     7561 cactatgcca agaagacagc tgaagctggc ttggatggtg ttgtttgctc ggctcaggaa
     7621 gtacaagtca tcaagcaggc taccaatcca gattttatct gtctgacacc agggattcgt
     7681 ccagctggtg ttgcagttgg agatcaaaaa cgagtcatga cacctgctga tgcctatcaa
     7741 atcggcagtg actatatcgt agtgggacgt cccattaccc aagctgagga tcctgttgca
     7801 gcttatcatg ccatcaagga tgaatggaca caggactgga attaaagaac tagattagaa
     7861 aaataaaagg agaataccat gacacttgct aaagatatcg ctagccacct cttgaaaatc
     7921 caagccgttt acctcaaacc agaggaaccc ttcacttggg catctggtat caagtcaccg
     7981 atttacactg ataatcgtgt gacactagcc tatccagaaa ctcgtaccct aattgaaaat
     8041 ggttttgtgg atgctatcaa agaagccttt cctgaagtag aagtgattgc aggaactgca
     8101 acagcaggga ttccacacgg agccattatt gctgataaga tggacttgcc ttttgcctac
     8161 atccgtagca aaccaaaaga ccatggagct ggtaatcaaa tcgaaggtcg cgtagctcaa
     8221 ggtcaaaaaa tggtagtggt tgaagacctt atttcaacgg gtggttcagt tcttgaagct
     8281 gtagcagcag ccaagcgaga aggagcagat gtgcttggag ttgtagcgat tttcagctac
     8341 caattgccaa aagcagataa gaactttgca gatgctggtg ttaaacttgt gacgctttca
     8401 aactatagcg agcttatcca tctagcccaa gaagaaggtt acatcacgcc agagggcctt
     8461 gatcttctaa aacgctttaa agaagaccaa aaaaattggc aagaaggtta ggtcagtaag
     8521 ataaagagag acgaggctac cgagcctctt ttaccatttt atttaaaa
//
LOCUS       DAVLTM010000081         8511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLTM010000081 DAVLTM010000000
VERSION     DAVLTM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.082"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            88..870
                     /gene="thiM"
                     /locus_tag="VYN16_001553"
     CDS             88..870
                     /gene="thiM"
                     /locus_tag="VYN16_001553"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1404061.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKHPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            872..1501
                     /locus_tag="VYN16_001554"
     CDS             872..1501
                     /locus_tag="VYN16_001554"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1404062.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      1710..1798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2060..2620
                     /locus_tag="VYN16_001555"
     CDS             2060..2620
                     /locus_tag="VYN16_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1404063.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            2621..4006
                     /locus_tag="VYN16_001556"
     CDS             2621..4006
                     /locus_tag="VYN16_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404064.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSNGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDYMVELRDGKLREVFQIPS
                     YEMTQVASKEVAASPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            4008..4658
                     /locus_tag="VYN16_001557"
     CDS             4008..4658
                     /locus_tag="VYN16_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1404065.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            4669..5361
                     /gene="tenA"
                     /locus_tag="VYN16_001558"
     CDS             4669..5361
                     /gene="tenA"
                     /locus_tag="VYN16_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1404066.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            5366..5890
                     /gene="thiW"
                     /locus_tag="VYN16_001559"
     CDS             5366..5890
                     /gene="thiW"
                     /locus_tag="VYN16_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1404067.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            5890..6693
                     /locus_tag="VYN16_001560"
     CDS             5890..6693
                     /locus_tag="VYN16_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1404068.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            6686..7318
                     /locus_tag="VYN16_001561"
     CDS             6686..7318
                     /locus_tag="VYN16_001561"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1404069.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7527..8318)
                     /gene="thiD"
                     /locus_tag="VYN16_001562"
     CDS             complement(7527..8318)
                     /gene="thiD"
                     /locus_tag="VYN16_001562"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1404070.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPESVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(8402..8500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         2509 a         1561 c         1848 g         2593 t
ORIGIN      
        1 tcaatgtaaa tgaaaaaaat tcttaacgaa agatagaaat aaatcgaaat cagtagatcg
       61 tataaagtga aaaggaagga tttcaatatg acaagtttaa aattattaaa agaaaaagca
      121 ccattggtca tttgtataac caatgatgta gtaaaaaatt tcacagcaaa tggattagta
      181 gcactgggcg cctcaccagc catgagtgag tttccagcag atttagagga tttgttaaag
      241 tatgctggtg gtttattaat aaacatagga acattgacag atgaaaattg gaaattatac
      301 caagctgctc tgaaaattgc ggagaaatat aatgtcccag cagttttaga tcctgtagcc
      361 tgtggagcag gagaatatag aaaaaaagta gcagatgatc taatcaacaa ttataaacta
      421 gcagcgatta gaggaaatgc tggcgagatt gcctctttag taggaataga tgtggcatct
      481 aaaggagtag atagtgcggg cgtagataat attgacgaaa ttgctctagc agcaaatgag
      541 aagttcaata ttccaatagt agtaacaggt gaagtggatg ccattgctgt taatggagaa
      601 gtggtaacga ttcataatgg tagtgctatg atgccgaaag tcattgggac aggatgctta
      661 ttaggagctg tagtagcaag ctttatcgga ctagaaaaag gtcaagaatt gaaatcatta
      721 gaaacagcaa tgttggttta caatatcgct ggagaaatag cagaaaaaca tccaaatgga
      781 catcttcctg ggacatttaa agttgaattt ataaatgcct tatacgagat tacagatgaa
      841 gatgtaaagg aattcaaaag agtgaagtaa gatgtttcat aaagaattac taaaactata
      901 ttttatttgt ggaacgacta cttgccaagg aaaaaatcta tatacagtcg ttgaggaagc
      961 cttaaaaggt ggtataacct tatttcaatt tcgagaaaaa ggtgagggtg ctttagaagg
     1021 cttagaaaaa cttgagctag ctatccaaat taaagaactt tgtaaaaaat acaatgttcc
     1081 atttatcgtt aatgacgata tagatttagc aatggaaatt gacgccgatg gcgtacatgt
     1141 aggtcaagat gatattggtg ttgatgaaat tagaaaattg atgccagata aaataattgg
     1201 tctttctata agaaacgaag aagaatttca acaatcaaaa gttgaatatg tagattatgt
     1261 aggtgttggg cctgtatttg gtacccagtc aaaggatgat gctggtggta ctataggtta
     1321 tgaaggtctt gaattgatga gaaaactatt gccacaaatg cctttagttg caattggtgg
     1381 gatacagacg aaacatatta aagacattat aaagaccaat atggatggcg tttcaatcat
     1441 ttcggcaata tcgtatgcaa aaaatataga aaaaactgtt cgggaaatga gtgaacaata
     1501 gatatctatc cttggattct aagtaagtag gattttcctt ttttaaaaag ctatgtccag
     1561 attcagcttg aatggctaat tcatcaaaca ggtcatttgt tttttgcaaa agaaaaatct
     1621 gctgtctttg tgagtgcaag gctgtatttc cagttcaatt tcttgaacta gtataaaatg
     1681 tgtgttataa taatcacata attaaataat aaagacattt ggggtgcttt aggctgagat
     1741 gatacccatt gaacctgata cagttaagac tggcgaaggg aaatgtgaaa tgtttttttc
     1801 gtatgtcgaa aagaactgtc taatcttgta agctgaactc taattcgtat ctgtaattag
     1861 agttttttct tttacgaaac tggatgaaga tgatatagta gattgaaata agatgcgaac
     1921 aaatcaatta taaaattcaa attaatttct agaaatattt tagtgttctt gtgtactgtt
     1981 ctaaattcag tctgctatac aaagtattta ggtctgtctt cttgagtgcc ccacttttct
     2041 ttaaaaaaag gagtttttta tgttgaaaaa atggcagtta aaagatgtta tcttgcttgc
     2101 tttcttgtct atcttttttg gtggggtttt cgttggttca ggatatgtgt ataatattct
     2161 cagtctactc ttaacacctc ttggtttgca ggcctttgcc aatgaaatcc tcttcggtct
     2221 ctggtgtatg gctgcgccca ttgctgccat ctttgttccg agagtcggaa gtgcaacgat
     2281 tggagaagtg ctagctgcgc ttgctgaagt cctttatggt agccaatttg gtctaggagc
     2341 tcttttgtct ggcttggttc aaggtttggg aagtgaattt ggttttatcg taactaagaa
     2401 tcgctatgaa agttggctct ctctaactgc taatagtatt gggattacgc ttgttagctt
     2461 tgtctatgaa tacattaagt taggctacta cgccttttcc cttccgtttg tcctttcctt
     2521 gcttgtggta cgttttattt ctgtttattt cttctgtacc atcttggttc gtgccattgt
     2581 caaactctat catcagtttg caactggagg aaaagcatag atgggtctgg aactacgagc
     2641 gattcagtcc ccaatcttct ctgagccgtt tgattttact tttcatgcgc aagcctttac
     2701 cttgttagtt gggagcaatg gttcaggaaa atccagcctc tttcaaatga ttgcccaagt
     2761 cagttctctt ccctatagtg gtcaagtcct gatagatggg agcgaggtca gtcagctttc
     2821 tatcatcgca cgtgtccaga aggttggcat tctcttgcaa aatcccaatc atcaatttac
     2881 catggagaac ttgtttgagg agctgatttt caccttggag aatatcggct atcaccttca
     2941 ggaaattgat tctaaaatag cagaggttgt ccagcaatgt cgttgcgagg caatcttaca
     3001 ccgtcccatt catcacctat caggtgggga aaagcaaaaa gcagcgctgg ctgtcctctt
     3061 tgccatgaat cctagggtct atctcttgga tgagcccttc gcttccattg accgcaagag
     3121 tagaatcgag atattggaga ttctaaaaga gttggctctt gatgggaaga cagtcatttt
     3181 gtgcgaccat gatttatctg actataaagc ctatatcgac tatatggttg agctaagaga
     3241 cggaaaacta agggaagtgt ttcaaatccc ttcctatgag atgacacagg ttgcttcaaa
     3301 ggaagttgct gctagcccgg aattattcca tatgaaccgt gtgactggtg agcttggtaa
     3361 tcgccccctc ttttcaattg ctgatttcac attctatcaa gggatttcct gtatcctggg
     3421 tgacaatggt gtcgggaaat caaccctctt tcggtctatt cttcaatttc aaaagtataa
     3481 ggggcgcatt gcatggaagg ggacagtcct gaaaaagaaa aagagtttgt accgtgatct
     3541 gacgggtgtt gttcaggaag ctgagaagca gtttatccga gtcagtctgc gagaggagct
     3601 tcaattagat ggacctgatt ctgaaagaaa tcagcggatt tttcaagctt tacgatattt
     3661 tgatttggag caggcagtcg ataagagtcc ctatcaatta agtggtggtc agcaaaaaat
     3721 tcttcagctc ctgaccatct tgaccagtaa ggcttccgtg atcttgctag atgaaccttt
     3781 tgcaggtttg gatgatagag cctgccatta tttttgcaag tggattgtgg aggagaggaa
     3841 ccaaggaaga agttttctgc tcattagtca tcgtttagac cctttgattt ctgtggttga
     3901 ttattggatt gagatgacta gtcagggtct tcggcatgtg aaagaagtga ccattaccaa
     3961 accacttaca tctcagagta gcaataccca aggggaggtg agatagtatg gtcaaagtag
     4021 caacccagac accgattatc agtctcttct tgctgatttt atccttggaa acatctttca
     4081 ttccttcgat tgctctgaat ctttcggtag tcgcattttg tattctcttt atgctctatt
     4141 accgtcgatt taaaatgtta gcttggatga tcatacttgc cattttacca tcttttgcca
     4201 actactgggc agttcagtta cacggagatg cttcacaggc agtcatgctt ggaacgaggg
     4261 cttttgtgac agtttgtatc ggccttgtct ttgtttcctc tatttcacta aaagagcttc
     4321 tcttgtactt ggctcaaaag gggctatcac gctcttggtc ctatgccttg attgtggtat
     4381 tcaattcctt tcctctcatt cagcaagaaa tcaagtccct caaagaagct tgcctattac
     4441 gtggtcaaga actacatttt tggtcgccct tgatttacag taaggttctg atgacagtct
     4501 ttaggtggcg ccatctttac ctgagagctc tatctgctca cggatatgac gaacatgcac
     4561 agttgaagaa tagctatcgg actttttata ttcctaaaaa aacaaaatta atctacctgc
     4621 ttttcttttt attgcttcaa accagtctat ttttataaag gagttattat ggaatttaca
     4681 gatattgcga tggaattatc caagaaagct tggcaggctt cctttcatca tccctttatt
     4741 ttacaattac aagaggggaa tttagaacct gccattttcc gctattacct gattcaggat
     4801 gcctactatc tgaaggcttt ctcagaaatc tatcatctgt tggctgataa gacttcaaac
     4861 caagagatga aaagactctt gaaacaaaat gctcagggtt tagtggaggg tgagttattt
     4921 attcgccaac aatttttcaa ggaattggaa atcaacgacc aggaaatgga gcaacatcca
     4981 atcgctccaa cctgttatca ttacatttct catatttata ggcaatttgc agaaccgaac
     5041 ttagccatag cttttgcaag cttgcttcct tgtccttggt tataccatga tataggcaaa
     5101 tcacttaatc ttaaaccatc accaaatcct ctctatcaac aatggattga aacttatatt
     5161 acggatgagt tagagcaaca gatcagagag gagggagcac tggtcaatca gctctatcga
     5221 gaaagtgatg agacagataa gcaaaaaatg ctagatgcct tccacatcag tgttcatatg
     5281 gaagccaagt tttgggagat ggcttaccaa caccagacat ggaagagcga tttacagtct
     5341 ttagaaaaag gagaagaata gaaacatgag aaagcaccaa ctacaagttc acaaattgac
     5401 cattttatct atgatgattg cccttgatgt agtccttaca cctatctttc ggattgaggg
     5461 aatggcaccg atgtccagtg tagtcaatat tctagcggga atcatgatgg gacctgttta
     5521 tgccttggct atggctacag tgacagcctt tatccgtatg acgactcaag ggattccgcc
     5581 tttagctctc acaggagcga cttttggagc ccttctagca ggtctctttt ataagtacgg
     5641 tcgaaaattt tacttttctg ccttgggaga aattgtggga acaggtatta ttggttctat
     5701 tgtttcctat cctgttatgg tactctttac aggatcagct gctaagctta gctggtttat
     5761 ctacacgcct cgatttttcg gagcaacctt gattggtaca gcgatttcct ttattgcctt
     5821 tcgattttta atcaagcagg aattctttaa aaaagtgcag ggatatttct ttagtgaaag
     5881 gatagactga tgcaggaatt tacaaatccc tttcctatag gctctagttc cctcattcac
     5941 tgtatgacca atgagatttc ttgtgagatg ctggcaaatg ggattttggc tctgggatgc
     6001 aaacctgtca tggcagatga ttcccgtgaa gttcttgatt ttactaagca aagtcaggct
     6061 ctcttcatca atttaggtca tttgtcagct gagaaggaga aagcaatccg catggcagct
     6121 tcgtatgcaa accaatcttc tctcccgatg gtagtagatg cggttggcgt aacgacttca
     6181 tccattcgta agagcttagt taaagacctt ttagactata gacctacggt ccttaaagga
     6241 aacatgtcag aaattcgaag tcttgttgga ttaaagcacc acggcgttgg ggtcgatgcg
     6301 agtgctaaag atcaagaaac ggaggatttg cttcaagtct tgaaagactg gtgtcagacc
     6361 tatcctggta tgtcattctt agttactggc cccaaggacc tcatcgtttc gaaaaatcag
     6421 gtcgctgtac tggagaatgg ctgtactgaa ttagactgga taacagggac aggagatttg
     6481 gttggagcct taacagccgt ttttctcagc caaggaaaga ctggttttga agcttcttgc
     6541 ttagcagtct cttatctcaa tatcgctgct gagaaaatag ttgttcaagg aatgggattg
     6601 gaagaatttc gttaccaagt actcaatcag ctttcgctcc taagaagaga tgaaaattgg
     6661 ctagatacca tcaaaggaga ggtttatgaa tagagaagca cttagactat atctggtaac
     6721 caatcgctat caagattccg tggaaagctt tcttgcaaaa gttgagacgg cctgccgttc
     6781 aggggttacc atagtccaat tgcgagaaaa aaatctcaca accaatcaat attatcaact
     6841 ggcaaaacaa gtcaaggaaa taacagatgc ttatcaggta cccttgataa tcgatgatcg
     6901 gttggatgtt tgtcttgcgg ttgatgctgc tggtctgcat attggagatg acgaactccc
     6961 agtttcggtt gcccgacaag tcttgggtcc tgaaaaaatc ctcggtgtca ccgctaaaac
     7021 ggttaaaaga gctctggaag cggagaaatc aggtgcagat tacttgggga caggagccat
     7081 ttttccgaca accaccaagg aaaatgcacc catcaccctg atttcaacct tgaaaacaat
     7141 ttgccaaacg gttgccattc caatagttgc tattggcggc ttgacttcgg agaatattga
     7201 tcagcttatg ggcactggca tagctggtgt agctgttgta cgtgatttga tgcaagcaga
     7261 agatattgag gcaaaaacgc aagccttttt aacaaagttg catgacattc tttcctaatt
     7321 aatactcaat gaaaatcaaa gagtaaacta gaaagctagt cgcaggttgc tcaaagtact
     7381 gccttgaggt tttagataag actgacgaag tcagctcaaa acagtgtttt gaagttgcag
     7441 atggaagctg acgtagtttg aagagatttt cgaagagtat aaaaactctc taattccctt
     7501 aaagtgggaa ctagagagtt tttttcttaa tctttaaaag ttgtatggtt gactggacca
     7561 gaaccatgac cgagttgagg ggcatcttga atagcttttg tgataaaggc cttggcctta
     7621 tcaactgcct ggtaaagact cttgcccttg gctagttcag cagtaatcac tgcagcaaag
     7681 gtacatccag taccatgggt gtgacaggtt tgaattcgtg ggctttccca gacaaattgt
     7741 tcattcttgg taaagaggaa atctttagca ccacctttga gatgtccgcc tttgataacc
     7801 acagactcag gaccaaattc ttttaaaatc aggcgaccag cacgctgcat gccttcgggg
     7861 tcatggattg aaaaaccaac aatctcttct gcttcaggaa gatttggcgt aataatagtt
     7921 gctagaggta gtaagtttgt tttgagatag tctctagcac ttgagtcaat caaggcatct
     7981 ccacttgtag caaccataac aggatcaagg acatagggac aatccagttt tttaagatag
     8041 ggttggatga tttccatgat ttcagtagta gccaacattc cagtttttac agcctgaggt
     8101 ggaatatcag aaaagacact ctccaattgg gctttcaaca tttgaggaga aacatgctcg
     8161 attagctgaa cacctctggt attttgagcg acaagactgg ttacaacagc cattccatag
     8221 acatctctcg cttggaatga ctttaaatct gccataatgc cagcaccacc actagggtca
     8281 gtccctgcaa tggtcaaagc aacgggtaaa taagtcatct aaaacctccc aaccaactga
     8341 agtttgtatt cttaagaaca agctagtctg ttttagaaag gcaatagaag actgctagtc
     8401 cccattatca tttccacttc catcagctag tattacctag attgagtcag agggatctaa
     8461 cagtcgttaa tctcagcttt acgcacccct agtggttgtt ttcttttttc t
//
LOCUS       DAVLTM010000082         8427 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLTM010000082 DAVLTM010000000
VERSION     DAVLTM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.083"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(31..684)
                     /gene="phoU"
                     /locus_tag="VYN16_001563"
     CDS             complement(31..684)
                     /gene="phoU"
                     /locus_tag="VYN16_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1404071.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(696..1454)
                     /gene="pstB"
                     /locus_tag="VYN16_001564"
     CDS             complement(696..1454)
                     /gene="pstB"
                     /locus_tag="VYN16_001564"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1404072.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(1467..2270)
                     /gene="pstB"
                     /locus_tag="VYN16_001565"
     CDS             complement(1467..2270)
                     /gene="pstB"
                     /locus_tag="VYN16_001565"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1404073.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2281..3165)
                     /gene="pstA"
                     /locus_tag="VYN16_001566"
     CDS             complement(2281..3165)
                     /gene="pstA"
                     /locus_tag="VYN16_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1404074.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3155..4072)
                     /gene="pstC"
                     /locus_tag="VYN16_001567"
     CDS             complement(3155..4072)
                     /gene="pstC"
                     /locus_tag="VYN16_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1404075.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGIWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4072..4950)
                     /gene="pstS"
                     /locus_tag="VYN16_001568"
     CDS             complement(4072..4950)
                     /gene="pstS"
                     /locus_tag="VYN16_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1404076.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5524..6828)
                     /locus_tag="VYN16_001569"
     CDS             complement(5524..6828)
                     /locus_tag="VYN16_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1404077.1"
                     /translation="MQFPEGFVEKYKEILGDEARNFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6829..7602)
                     /locus_tag="VYN16_001570"
     CDS             complement(6829..7602)
                     /locus_tag="VYN16_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1404078.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7592..7870)
                     /locus_tag="VYN16_001571"
     CDS             complement(7592..7870)
                     /locus_tag="VYN16_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1404079.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7870..8271)
                     /locus_tag="VYN16_001572"
     CDS             complement(7870..8271)
                     /locus_tag="VYN16_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1404080.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         2510 a         1983 c         1576 g         2358 t
ORIGIN      
        1 aagctccttt ttatacagaa ttaaactatt ttatagttcg acaatcttac ctgtttcaaa
       61 gtagacaacc cattcacaga tatttttagc atagtcaccg atacgctcca agtaggaaat
      121 aacttggaaa taatcacgac ccgtaacaat ggcttctgga tttttcttaa tctcttcagt
      181 cgcaaggtca cggatagttt caaaatagtg gttaatttgc tcatccatgg aggccacccg
      241 gtatgcgtcg tcaacagaac cattaagata aagatcaagt gctgcttcca caacgctttt
      301 aacttcacgt cccatttttt taatttcttc ctctacagct gggatgcgtt cttccccctt
      361 catacggatg gttgcctggg caatggctac agcgtgatcc cccatacgct ccacatctga
      421 tacagcctta aggacagtca agactgtacg caaatcttga gagactggtt gttggagtgc
      481 gatcatttca aatgatttct tttccagttt cacttcgtat tcatttactt ctgcatcatc
      541 ttcgatgacc tcttttgcca ggtcacggtc atgcgtgaca aaagcacgta ccgtacgatt
      601 gatttgtgag agcacttctt gtcccatagc gtagaactgg ttatgtaatt tctctaaatc
      661 ttcttcaaat tgagatcgta acatctttca tctccttatc caaattttcc tgtaatatag
      721 tcttccgttt ccttgtgttg gggatcaagg aacatctgct tggtatcatt aaattcaatc
      781 aaatctccat ctaggaaaaa tcctgtctta tcagagatac gtgaagcttg ctgcatggaa
      841 cgggttacca gaagcatggt gtacttgtct tttagaccat acaaggtttc ctcaatttta
      901 ccagctgaaa tcggatccaa agccgaagtt ggctcatcca agaggatgat tttaggacta
      961 gttgccaaga cacgggccac gcagacacgc tgctgttgac cacctgacaa tccaatagct
     1021 gaatcatata gacgatcctt gacctcatcc cagatagagg caccttgcaa ggctttttct
     1081 acggcttcat ccagaacctg cttatcctta attccattga tacgaagccc gtagacaaca
     1141 ttctcataga tagtcatagg gaaaggatta ggttgttgga aaaccattcc gatttcctta
     1201 cgtaattcaa ccgtatctgt acgcggactg tagatgttgt gaccattgta caccacggat
     1261 ccagttgtgg tcacctctgg attgagatct cccatgcggt tgagagactt gaggagggtt
     1321 gacttccctg atccagatgg accaatcaag gctgtaattt ccttaggttg gaaagatagg
     1381 gaaacactat tcaaagcctt ctttttatta taataaacgg acaggtctga tacctgtaaa
     1441 atcgcatctg tcatacggtt tcctttctaa ccaaagtgac cagatacata gtcattggtg
     1501 gactgtagct tggcattttg gaaaatagtt gcagtcttgt catactcaat caaatcaccc
     1561 aagtaaaaga agcctgtata gtcacttgca cgagcagcct gctgcatatt atgcgttaca
     1621 atgatgatgg taaagttttt cttgagctca aacatggtct cttctagttg catggtcgca
     1681 atcggatcca aggctgaggc tggctcatcc attaagagga tatctggctt aacagagatg
     1741 gcacgagcga tacagagacg ttgttgctga ccacctgata aggtcaaggc tgacttgtgg
     1801 agatcgtctt taacctgatc ccagagggca gcctgacgaa gggaggtttc tacgatttca
     1861 tctaggactt gcttatcctt aactccagca cgttcatgcg caaaggtaat attacggtaa
     1921 attgacttag caaatggatt ggggcgttga aaaaccattc caatgtgttt acgcatttca
     1981 taaacgttga tttctggacg gttgacatca attccacgat agagaatctg cccagttact
     2041 ttagcaatat caatggtatc attcatgcga ttgagactgc gtaagtaggt agatttcccc
     2101 gatcccgacg gaccaatcaa agctgtaatt ttatttcttt caaattgcat atcaatcccc
     2161 ttaatggatt catttttacc atagtaaaca tggacatcct tagtagaaag ggctactttt
     2221 tcttcaggaa aggtaaggat atgcttctca tcccagttat atgttgacat ggcttctcct
     2281 ttaggcagcg gttaatttct tgtgtagata gcttccgaac ttacgagctc caaagttaaa
     2341 aatcaggata aagatcagga gcacagcggc agaacctgct gatacaatgg ttccatctgg
     2401 aatagtgcct tcactattga ctttccagat atggacagcc aaggtttctg cttgacggaa
     2461 gatagagatg gggctagtca cactgaggat attccagtta gaccagtcaa gagctggcgc
     2521 cgattgccct gctgtataga tcagagctgc agcttcgcca aagatacgac cagatgccaa
     2581 gacgacaccc gttacaatac ctggaagcgc ttctggaata acaacatgaa ccactgtctc
     2641 ccagcgagaa atcccaagag ccagaccagc ctcacgttgg gtatggtgaa cgtgtttcaa
     2701 actatcctct acattacgcg tcatctgagg caagttaaag actgtcaagg ccaaggcacc
     2761 tgaaatgatt gaaaatccat actcaaactg aactacaaag atcaagtaac caaagagacc
     2821 caccaccact gatggtaaag aggacaagat ttcaatacaa gtccgcacaa agttggtaac
     2881 aggacctttt ttagcatatt cagccaagta aatcccagct cccatagaaa gaggtacaga
     2941 aataatcaag gtaatgacca ataggaaaaa ggaattgtaa agctgaatgc caatcccacc
     3001 acctgcttga aaagcagaag accttccagt caagaaagac caagagatat ggggcaagcc
     3061 ccgaaccaag atatagagaa tcaaggaagc caagattgtc acaatgatgc tagcaatcgt
     3121 atagaggaca gctgttgcaa gtttatctaa tttcttagcg cgcataattt ttctttcctc
     3181 tttctttcgt aatcaattta atcacactgt taaaaactaa actcatcaag agcagtacca
     3241 aggccagtga ccagagaaca ttattattta cagttcccat gacagtgttc ccaattccca
     3301 tagttaatat agaagttaaa gttgcagctg gtgtggtcaa ggaagttggg ataacagctg
     3361 agtttccgac aaccatctgg atagctaaag cctcaccaaa ggcacgcgcc atcccaaaga
     3421 ccactgcagt gaaaatacca gaacgggccg ccttcaagat cacacgccag atagtctgcc
     3481 agcgagtggc tcccatagcg aaactggctt cacgataata acgaggaacc gcacgcaagc
     3541 tatccgttgt cataaaggtt acggtcggca aaatcatgac aaagaggacg gaaatccctg
     3601 acaaaatccc aaaaccagtc ccaccaaaga cactgcgaac aaagggaacg acgacttgca
     3661 agccaataaa tccgtacact actgaaggaa tcccaaccag gagttcaata gctggttgca
     3721 aaatcttcgc cccttttggt gatacttcgg tcataaaaac tgctgcacca atagcaaagg
     3781 gggttgcgat aagggctgag agaatggtaa cgataaagga acccaaaatc ataggaaggg
     3841 caccaaattc tttactagaa ggattccaaa ttcctcccaa aagaaagtca aagatattca
     3901 caccattgac aaagaaggtc gacaagcctt tttgcgctac gaaaaccaaa atcatggcca
     3961 caaggatgac tatcaaagaa agacaggcaa aggtcaaacc ttttcctaat ttctccagac
     4021 gagaattctt tgatggaagc aacattttct tagctaattc ttcttgattc attattgtct
     4081 cccttccaac actgtcacag ttccggcagc atctttttca accttcattt ccttaatcgg
     4141 aatatacttc aatcctttga caatcccttc ttgggtctca tccgagagaa caaaattgag
     4201 aaattctgca gccaactcat tgggctgccc caatgtatac atatgctcat aagaccacaa
     4261 gggccaatta ttgctactta tattttctgg acttaagtca tagccattca acttcatgct
     4321 tttgaccgaa tcatctatat aggtaagaga taaataagag atagctcctg gactttttga
     4381 tacgattgat tttaccgctc catttgaatc ctgctcctga ctttgcatgg cagactgacc
     4441 ttccataatg acagtatcaa aggtagcacg agaaccagag ccggctgccc gattgataac
     4501 agagatgggt aagtccttac caccaacctc tttccaattg gttacctcac ctatgaagat
     4561 ttgacgaagt tgctctgtcg ttaggttatc aacatcaacc tccttattga caatcagagc
     4621 caagccagct accgcgacct tgtggtcaac aagagcagaa gcatcaattc cgtctttttc
     4681 ctcagcaaat acatctgagt ttcctatatc aactgcccca gactgaacct gggacaagcc
     4741 tgtaccagaa cctccccctt ggacattgac cgtttttcca acatggatcg tgccaaattc
     4801 atctgccgct acttcaacca agggttgcaa ggcagttgag ccaacagccg ttatggattc
     4861 tccacgatca atccagctag cacagcctac taaacaagcc gtcagccaaa aagcgataag
     4921 agacagagca agcttttttc tttttttcac tgtttttctc ctcgaaaata attatgaata
     4981 ctgtgaattt tttaagtagt tctttatgag ttgacgcatg aattcttacc aaatttctgc
     5041 gcaattgatt atttatataa tataggctat attactcttt cctaacctcc ttttttcata
     5101 tgtggataaa atctcttgtc tatcccttcc cccattgtca cccattatag tcatttcgtg
     5161 tctctttttc ccctttttaa tgcaagggaa attactctcc ttagatgata atccaaaagc
     5221 tagaaaggta tctcaaacct ctctactctc ccagactagt ttacaactaa aaggaaaaga
     5281 ttctatttta tgagaaatct agtttacaag cggtaagaac gctaataact aaacttcttg
     5341 tactctttga aaatctcttc aaaccagtgt tttgagctat ctatggctag cttcctagtt
     5401 tgctctttga ttttcattga gtagtaaaac tacatgtaat ggcaatcaag atatcaagaa
     5461 tcatcctact aaaaaaatcc atactttcac tataacatag aataagatat ttgactagca
     5521 ttttcatttg aatctgaggc cttttggaaa ataatttttc aaaacatttc cagtaacctt
     5581 tgcaaagccc aagccattgc ctttaaccaa aacttggtac caaccatttg gcagactttc
     5641 tgccagctga acgatttctc cagccgcata cttgacaaac gcttcttggc caatttcaac
     5701 cgactgttcg acctgactcg atttcaaggc taaaccaaga gcgaaactgg gctcaaagcg
     5761 tttcttctta aaagtaccca gatgcagtcc attgcgagca atcttgagct tccctaaatc
     5821 tggcaaaagt tctggcaaga gataaagctg gtctccaaaa gtctgcaaga tacccggtag
     5881 attgaccttc aaatggtttt gggcaaattc ctgccacaag gcaacttgtt cacggctgag
     5941 gttactctta cttgccttaa atttaggagc tggattgtta cccttaaact gtagatgggc
     6001 aacaaactga ccctctccct taaactgatg agggtacatc cgagccgttt ctggcaggtc
     6061 aataccagct accattccat tgatatgctc tactggcaac aagtcaaaat catactcttc
     6121 cagcaaccaa ttgacaatct cttcgttttc ctcgggtgcc caggtacagg tcgaataaac
     6181 cagacgacca ccttcagcta acatggtcac tgcatcctcc agaatttctc tttgcaagct
     6241 agcacattga ctcggataat ctaagctcca atagtccata gcatcaggtt gcttacgaaa
     6301 cattccttca ccagagcaag gggcatcaag aacgattaag tcaaaatagc ctttaaagac
     6361 cttgaccaag cggtcggcag attcattggt caccacgaca tttgtcgctc caaaacgctc
     6421 catgttttca accaaaatct tagcccgttt gcttgaaatt tcattggaaa caagtagccc
     6481 ctcccctgct agataggctg ccagttgagt tgatttgccc cccggtgcag cagccaagtc
     6541 caagaccttc ataccaggac tgggttgggc tacttgagcc accatttgag cagcaggttc
     6601 ttgcgaataa actaaacctg tagcatgctc aggcgatttc cctgaaacct tcccatagtg
     6661 gccccaaggg gtttgagtaa tggcatcaga aaaggaaagt tgctcttctt ttaagggatt
     6721 gacccgaaag gccgaaaccg cttcctcctc aaaagaggca agaaaatttc ttgcctcatc
     6781 tcctagtatt tctttatatt tttcaacaaa tccttctgga aattgcattt aagttctttt
     6841 cctttcgtaa atataggact gaatttcctc ctgcatctca agaggcacca tcatgacagg
     6901 ctgtctggtt tgaaaatcag gagcttcacc aaaaagggtc acaacccgat agcccagact
     6961 ttcccctaaa atactagctg cggcataatc ccatggttgc agataagtga gataggtcaa
     7021 caaacgccct gacaaaatct tggcaaaact aatggccgca cttccataga cacgaacacc
     7081 aagaaccgct cggctcaaat cagccagccc ccattcattg gtttccagca taccactatt
     7141 ccctgcaatg agaaaatctc caagtggttt agttttaaaa ggagctaggg gcctatcatt
     7201 tagacaaact ggaaattccc caccaccgtg gtaacaatcc cctttgacca catcataaat
     7261 cagaccaaac tgtccctgac cattttcaaa ataagccatc ataacagcaa aatcttcctg
     7321 ctgggctaca aaattattgg taccatcaat gggatcaatg acccaaacct tgccctcttg
     7381 aaccgaggct cgcagacaac cttcttcagc acaaatctta tcctcaggat aacgggacaa
     7441 aatctcacca accaagagtt cctgaacttc tttgtccagt ctagtcacca aatctgttgg
     7501 agaggacttg gtttcaacac gcaagtcttc ctgcatatgg tcaagaatgt actgacctgc
     7561 tttcttaaca agctctttag caaattcaaa tttactttcc aagagaaatc tttccttccc
     7621 ctttttcttt ggcagcctga actgctcggt aaagtgaata gccactaacc gtttcaaatt
     7681 ctcgtcccaa acgtttctct tcgctcttgc ttggcacgac cgctttgaat cccttatagg
     7741 agtccagtaa ctttttagcc tctactttgc cttcataggc agcttcaaca tcattaaaaa
     7801 aagaaagcac tgaagcaagt tcttcagtgc tccacgacaa atctagtggg taactatact
     7861 gtttgttcat taactaatac cagctctcat tcttgcttct tttagttctt gcttacgata
     7921 actacgaggg agaaaagcac gaatctcatc ttcattaaaa ccgatttgca tacgcttggc
     7981 atcaataata attggacgac gcaaaagact aggatactgc tcaatcaaat gaagcaattc
     8041 cgataccgaa atactctcta catcaatatt caatttttga aaaatttttg aacgagttga
     8101 aatgatgtca tcagtaccat tttcggtcaa ggaaaggatg tgttgcaatt cttttcttgt
     8161 taaaggactg gtcataatat tgtgttccac aaagggaacc ttatgttttt ctaaccaggc
     8221 cttagcctta cgacatgatg tacagctcgg tgatagaaat agtgtaatca tgcttttctc
     8281 ttcttatcta tactttgcta cttctattat acaaaaaaat aaagcgcttg actagggatt
     8341 tttagaaaaa aagcctattt tttcaagaaa aataggcttt ttgcgaacga ttgacacaat
     8401 tggatttggt taattcactc ttaacga
//
LOCUS       DAVLTM010000083         8342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLTM010000083 DAVLTM010000000
VERSION     DAVLTM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.084"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(161..364)
                     /locus_tag="VYN16_001573"
     CDS             complement(161..364)
                     /locus_tag="VYN16_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404081.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(357..1439)
                     /locus_tag="VYN16_001574"
     CDS             complement(357..1439)
                     /locus_tag="VYN16_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1404082.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1565..2347)
                     /locus_tag="VYN16_001575"
     CDS             complement(1565..2347)
                     /locus_tag="VYN16_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1404083.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2347..3690)
                     /locus_tag="VYN16_001576"
     CDS             complement(2347..3690)
                     /locus_tag="VYN16_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1404084.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3789..5105)
                     /locus_tag="VYN16_001577"
     CDS             complement(3789..5105)
                     /locus_tag="VYN16_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1404085.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAANLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5298..6524
                     /locus_tag="VYN16_001578"
     CDS             5298..6524
                     /locus_tag="VYN16_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1404086.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6723..8297
                     /locus_tag="VYN16_001579"
     CDS             6723..8297
                     /locus_tag="VYN16_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1404087.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LNEKQTGFVIRNKGDK"
BASE COUNT         2373 a         1623 c         1857 g         2489 t
ORIGIN      
        1 tctgtgcagt acagaactaa atcctttgga taaattaatt gtctaacttt tggggggcag
       61 tacattttaa cacttgataa tatgcgtttt attgtgggaa gattttcctc attttctagt
      121 gaaattgaac tttttctaac atcttttgat agatgacttc ttaatcaatt atcatttcca
      181 ttgttgtttc ttggggtttc atcccttgtc tttgataaaa gcggagagca ccctcgttat
      241 cattccagac acttagggtt agattattgc agccaatttc tttagcataa tagcagaaaa
      301 atgacaataa aatagcccct agatattttg aaaggaaagc ataggggcta tatagtttaa
      361 tcaaaataaa gcaaatcttt gctggtgctt gtaaagaggt cgaagccatc cttggtaaca
      421 acaccgcagt cttcaatacg aacaccgact ttaccaggga tatagatacc tggttcaaca
      481 gagaagcaca tgccttcttc gatgaccatg tcgtttcctt ccatgataga tgggaattca
      541 tggacatcca taccgatacc atgcccgaga cggtggttga agtactcacc ataaccagct
      601 ttttcgatga cctcacgggc agcgcggtcc acttcatgag cagtcacacc tggcttgata
      661 aagtcaagag cagcttgttg ggcttcaaga gtcaagttgt aaatatcttt cttgaattgg
      721 tctggtttgc cgacagcgac tgtacgagtc atatctgacg catagccatt gaccagaaca
      781 cccaggtcaa agaggagaag agcatcattt tcaaccttat tagctgctgg aatgccgtgt
      841 ggattcgcag cattatcacc agtcaagacc atggtatcaa agctcatttc gtaaccttca
      901 cgtttcatgg caaagtcaat ttgtgcgatg atatctgtct cagtcttatc aagagaaata
      961 ttgtcaaaac caacatgaac agccttgtca gcataaagac ctgcaaccat cattttttgc
     1021 acttcatcag ctgatttgat gaggcgcatg cgttggatac gaggagtgag gttgtcaaac
     1081 tcagcagtct caaaaactgt tttcaaacca tggtatttgg tcaagatgag attgtcaaac
     1141 tcaacagcga cacgtttgaa gtcaagttgt ggaagagcat gtttgatttt ttgccatgga
     1201 ttttcagaat cgacatagcc cactactggg aaggaaacgg tgctacttgc acgttctact
     1261 tcaagagctg ggacaaagag gagaggttcc tgatccgcta ggacaaagag gaacatttgg
     1321 cgttcatggg gatcactgta aaaaccagtg aggtaattga ttgtgacggg gtcagatacg
     1381 acagcgacgt ctagtttttc tgattcaaga tatgttagga tttgttgtaa tttagacata
     1441 tgctaccttc tttctacccc tctattttgg caaaaaatga gagaaaatgc aagggagaag
     1501 ggtaaaagaa aagacaaaaa actccgacaa gataacggag ttctttgata tcattttcct
     1561 ttgtttaaga aaagtcagcc ttgcttttga cgtcactgta ctcttcagcg atttcttggc
     1621 tgagaatgtc ctcataggca gggagttgga tgtcctgacc atatttcttc ttgagggcag
     1681 tagtaactag gcgataagcc agattggcat tacgagagag gataggcccg tggaagtagg
     1741 aaccaaagac attcttataa tgaacccctt caccgacctt ttcttcgttg tttccatttc
     1801 catagacaac ctgtcccagc ggtttttggt catcagagag gaaggtacga ccttggtgat
     1861 tttcaaatcc atagtaggtt tcatcgaaat cttcattgtg aatcttgatg tcaccgataa
     1921 aacggttatt ggtctggttg agcgtgtagt gtcccatgac ccctagccct tcgatacgtt
     1981 ttcctgaagc ttcaacataa tattgaccca atagttggaa accaccgcag atagccagaa
     2041 ctacaccgtc gttttggatg tagttgtcaa tgctctcttt tttagcaggt aggtcgtctg
     2101 caatgatact ttgttcaaag tcttgaccac caccgaaaaa ggcgatgtcg tagtgatttt
     2161 catcaaagtc atcatggaga gaaacgatgt caacggtcac atgggctccc agtttttcag
     2221 ccacatactt gagcatgagg atgtttccat tgtccccgta ggtattcatg agatttccgt
     2281 agaggtgggc aatgttgagc tgataggggt aattgctatc ttttgaggaa agtgaagtat
     2341 aaaccattag ttcatctcct ttctaacaat ctgacgacta gccagcagtt cacgaaattc
     2401 cagcatggca gtataagttg ccagaatata ggcatgcttg cagtcttgat tctcaatggt
     2461 cttgagaact tgctccagat tactcgtttc agtgattttc tcagctggat agccagtcac
     2521 tcggaggcga cgagcgattt cagaatgacg aacaccgcca gcgttgattt caggaatgtc
     2581 catgtcagtg atttgctcaa agtctgcatc ccagatccag ctagtgtcaa ttccatctgc
     2641 atagttggca ttaaggagga cagataggct aaatggataa ggtgctagtt tgatcatttc
     2701 gatagcttgg gttgcaccga ctggattttt aatcaagaca agggtacatt ccttgtcacc
     2761 gatatgaaag gtttcttggc gtccaaagac agcacggctc ttgtcaaatc cctgtttgat
     2821 gagttgcgaa tcggcaccta ggaaacgggc gatggccaca gcagctaggg cgttatagat
     2881 attatagagc ccgccgattt ggataccgta ttcttggccg tctatgacaa agcgagagcg
     2941 attgttggtc aactcaacca attttgtcaa acgatagtcg agatcaggac gtttacatcc
     3001 acaaccttca cagatatagg cacccaagtt tgcataggta ttatgctcat atttgaggat
     3061 gccttggcag tcaggacaga gaatcccttc ggtattgtag tgagccagtt gggctggtcc
     3121 tttttccaag tcaaaaccaa aatactctat agggtttgga atagttggct tgtagaaaag
     3181 tggactgtct ccgttaagga gaacagtagc agttggaact ttccgaatgg catccaatat
     3241 catgttatag gtagtataga tttcaccgaa acggtccatc tggtcacgga agatattagt
     3301 aatgacaaaa agactaggct ggatatagtc acagatacga gatagactgg cttcgtcaat
     3361 ttcgaggacg gcaatatttt ttccagtttt agaagatttg gctgttagga aggttgttgc
     3421 aatccctgta atcatgttgg caccgcttgg attggttaga acttgaccat agacctcttt
     3481 taaaatgccg acagtgaggg cagttgtcag ggtttttcca tttgttccag tgacaacgac
     3541 aatctcgtag ttcttagcta ggttttgtaa aatatcttta tcaaattgaa gggcgacttt
     3601 acctgggagc gtacttccac ggccaagacg gcttaaaatg aagtgggaag aacgcccagc
     3661 aagaaggccc aaagtagttt ttaagttcat gtttctatta tatcataaaa gaggagaaag
     3721 acagatttga gattccgcag aagtaaaaac agcccaaaga gggctgttcg atacgattaa
     3781 atctcaactt aaatcgcaaa caagtcattc aagttctcag ccaaggttgg gtgagtgaag
     3841 atttgttttg tgaagtaagt gtaaggaatc ttgttgtcca tagcaacagt aataatgttg
     3901 atgatttctt gagaaccttc tgagaagatg cttgcaccaa gaatttcttt tgtttcagta
     3961 ttaacaacag ctttgaaagc tccgcgaagg tctccattta cgtgaccacg aggcatggct
     4021 gcaacaggga tttctttcac tgcgtatgga agtttcaaat tagctgcttg gctttcagtc
     4081 aaaccaactt gtgaaagtgc aggtgtgatg aacatagtat ttggcacatt gagacggtcc
     4141 tcaagtgtgt agctgccatc tccagcaagg tagctgtaaa caacacggaa gtcatcaagt
     4201 gaaatgtaag taaattgaag accaccgttg acatctccaa ctgcaaagac accaggaacg
     4261 tttgtttgac agtgtttatc tactttaata gcgccacgtt cagttagttc aatatctgta
     4321 ttttcaagtt gaagtggttc tacatttggt ttgcgtccag ttgcgtagag aagggcgtcg
     4381 aaacggtaag tttcgtcttc agttacgaca agcacttggt caccgtcgtt tttaatttca
     4441 gtagtatgga tattttgaag caattcaatg ccatcttctt ccatgtattg tttagcaaga
     4501 gctgcgatgg aaggttctgc acgaggaagg aaagtatcca aggcatctag gactgtgacc
     4561 ttgcttccaa gtttgttgta aaggccggca aattcaagac cgatatttcc gccaccaagg
     4621 attccaagtt tttcaggtaa tttgtccaag ttttggatac ctgttgagtc aaagatgttt
     4681 ttgcttgtag caagtccagg gattggcaag acgtttgaaa cagcaccagt gttgatgacg
     4741 attgtttcag cagtcagttc tttcttttca tcaccagctt ggatttcgat gactttattt
     4801 gaaaggaagt gagcttccgc atcaaagata tctacgcctg taccagcaac agtcgcatag
     4861 tttttaccgt tgaggcgacc agtgatcgtg tttttagtag caatcacttc ttcaaaagac
     4921 aagtcctttt cagcagcaac tagcaaggtt ttagttggga tacaaccaat gttgatacaa
     4981 gttccaccgt acatagcctt gctgcgttca acgagggcaa cttttttgcc agctgaagcc
     5041 aatttacctg ctagtgtttt accagcttta ccaaatccaa taacaattaa atcgtatgtt
     5101 aacatttatc ttcctcctat tcgaatttca ttctagcaca agaaaaaaac ttttgcacaa
     5161 ttctgctacg gaaaagacag aaaaagataa aaacgctttc cagacaaaaa actagtaatt
     5221 ttttgacaag cttgataaac taaaatccta tctattttta tataagatag aataaatgtt
     5281 cgaaaatagg aggttttatg aaatcgaatc gttatattat tgcctttgct ggggtcattt
     5341 tacacttaat gctgggttcg acttatgctt ggagtgttta tcgtaaccct attattgaaa
     5401 aaacgggatg ggatcaggct tctgttgcct ttgcctttag tctagcaatc ttttgtttgg
     5461 gcttatcggc tgcatttatg gggcgtttgg tagaaaaatt tggtccgaga gtcatgggga
     5521 gtctatctgc ttttctatac gcaggtggaa atatcttaac aggatttgca atagaccgtc
     5581 aggaactgtg gttgttgtat ctagcttatg gcattttagg tgggcttggt ttgggagcag
     5641 gctatattac ccctgtgtca acgattataa aatggtttcc tgataaacgt ggtctcgcaa
     5701 caggtttagc gattatgggg tttggttttg cttctttatt gactagtccc atagcgcaac
     5761 atctcatcgc aggggtaggg cttgtagaaa ctttttatat tttaggtgca agttacttta
     5821 ttatcatgct cctagcttca caattcatta agcgtccaaa tgagcaagag cttgcaattt
     5881 tatctgcttc agggaaagaa aaaacagact ctctgacgca agggatgact gcaaatcagg
     5941 ctctaaaaag caatcggttt tatatacttt ggattatttt ctttatcaac atagcttgtg
     6001 gtttaggctt aatttcagcg gcatcgccaa tggcacagga gatggctggc ttgtctacaa
     6061 gtcatgcagc agtaatggtg ggcgttttag ggattttcaa tggatttggt cgcttgctct
     6121 gggcgagctt atctgactat atcggtcgcc ctctaacctt tagtatatta ctgcttgtta
     6181 atcttttctt ttctctctca ctttggctct ttacagattc cgttttattt gtagtcgcta
     6241 tgtctatttt gatgacttgc tatggagctg gtttttcatt gatcccagct tatctcagtg
     6301 atatttttgg aaccaaggaa ttggccgctc tgcatggata tattttaaca gcttgggcaa
     6361 tggctggttt agcgggacct attttattag cagagactta taaaatggct cattcgtaca
     6421 cacaaacctt gttcgttttt ctcattttat acagtatcgc cttggctttg tcttattatc
     6481 taggtcgttc aatcaaaaaa gaaagtcaaa aaccgcttac atgattttct gtagaaatgg
     6541 ggctatcttt tgctctggtc ttgtgttata ctagataggt tgcaaagaaa acagtacttt
     6601 tcttttgtgg aaaagaagca acacgatttt atatctagtt atatgaaaat acgtcataaa
     6661 aagaaaagta taaactaagc cctatagggt ggcttcgcac cacctttaga aagaagaata
     6721 acgtgaaatt taatgaatta aacttgtctg ctgatttgct agcagaaatt gaaaaagctg
     6781 gttttgtaga agctagtcct atccaagaac aaactattcc tttggccctt gaaggcaagg
     6841 atgttatcgg tcaagctcag actggtacag gaaaaactgc agcctttggc ttgcctaccc
     6901 ttgaaaaaat ccgtacagaa gaagcgacta tccaagcttt ggtcatcgct ccaactcgtg
     6961 aactagctgt ccaaagtcaa gaagaactct tccgctttgg tcgtagtaag ggagtcaaag
     7021 tccgttcagt atatggcgga tcaagcattg aaaaacaaat taaggctctt aaatctggtg
     7081 cccatatcgt ggtgggaact ccaggtcgcc tcttggactt gattaaacgc aaggccttga
     7141 aattacaaga cattgaaacc cttatccttg acgaagcgga tgaaatgctt aacatgggct
     7201 tccttgaaga catcgaagcc attatttccc gtgtacctga gaaccgtcaa actttgcttt
     7261 tctcagcaac tatgccagat gccatcaaac gtatcggtgt tcagtttatg aaagcccctg
     7321 aacatgtcaa gattgcggct aaggaattga caacagaatt ggttgaccag tactatatcc
     7381 gtgttaagga acaagaaaaa tttgacacca tgactcgtct catggatgtg gcacaaccag
     7441 aactcgctat tgtatttggt cgtaccaaac gccgtgtgga tgaattgact cgtggtttga
     7501 aaattcgtgg cttccgtgca gaaggaattc atggcgacct agaccaaaac aaacgtcttc
     7561 gtgtccttcg tgactttaaa aatggcaatc ttgatgtttt ggttgcgaca gacgttgcag
     7621 cgcgtggttt ggatatttca ggtgtgaccc atgtctacaa ctacgatatt ccacaagatc
     7681 ctgagagtta tgttcaccgt atcggtcgta caggtcgtgc tggtaagtca ggtcaatcta
     7741 ttacttttgt tgctccaaac gaaatgggtt accttcaaat cattgaaaac ttgactaaga
     7801 aacgcatgaa aggtctcaaa cctgcaagtg tagaagaatc cttccaatca aaaaaacagg
     7861 tagctctcaa gaaaatcgaa cgtgattttg cagatgaaac cattcgtgcc aactttgaga
     7921 aatttggtaa ggatgctcgc aaattggctg ctgagtttac tccagaagaa ttggcaatgt
     7981 atatcttgag tctgacagtc caagacccag atagccttcc agaagtggag attgcacgtg
     8041 aaaaaccact accgtttaaa ccatcaggta atggtttcgg tggtaaagct aagggaggtc
     8101 gtggaggccg tcgtggggat gaccgtcgag agcgtgatcg ccgtggcaat ggtcgccgtg
     8161 atgagttcaa aaaaggaagt cgtggcaacg atcgttttga taaggaaaaa cgttaccgta
     8221 aggataataa aaaaccacgc aatactttaa acgaaaagca aacaggcttt gttattcgta
     8281 acaaaggtga taaataagaa aaagcggttc aaaatgaatc gcttttttat ataaggagac
     8341 cg
//
LOCUS       DAVLTM010000084         8145 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLTM010000084 DAVLTM010000000
VERSION     DAVLTM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.085"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            45..815
                     /gene="sufC"
                     /locus_tag="VYN16_001580"
     CDS             45..815
                     /gene="sufC"
                     /locus_tag="VYN16_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1404088.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            843..2105
                     /gene="sufD"
                     /locus_tag="VYN16_001581"
     CDS             843..2105
                     /gene="sufD"
                     /locus_tag="VYN16_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1404089.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            2116..3342
                     /locus_tag="VYN16_001582"
     CDS             2116..3342
                     /locus_tag="VYN16_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1404090.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDRVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            3329..3769
                     /locus_tag="VYN16_001583"
     CDS             3329..3769
                     /locus_tag="VYN16_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1404091.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            3823..5235
                     /gene="sufB"
                     /locus_tag="VYN16_001584"
     CDS             3823..5235
                     /gene="sufB"
                     /locus_tag="VYN16_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1404092.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(5466..6707)
                     /locus_tag="VYN16_001585"
     CDS             complement(5466..6707)
                     /locus_tag="VYN16_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1404093.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIEQI
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            6810..7919
                     /locus_tag="VYN16_001586"
     CDS             6810..7919
                     /locus_tag="VYN16_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1404094.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         2421 a         1488 c         1776 g         2460 t
ORIGIN      
        1 ttctagtaag tactgtaaaa gttttgaaaa agaaaggaac tatcatgtca gtaatagaga
       61 tcaaagatct tcacgttgag attgaaggaa aagaaatttt aaaaggggtt aacctgaccc
      121 tgaaaacagg agaaattgcc gctatcatgg gaccaaatgg tacaggtaaa tcgactcttt
      181 ctgccgctat catgggaaat ccaaactatg aagtaactaa aggtgaagtt ttgtttgatg
      241 gcgtaaacat ccttgagttg gaagtggatg agcgtgcgcg tatgggactt ttccttgcta
      301 tgcaataccc atcagaaatc cctggaatta ccaatgctga gtttcttcgt gccgctatga
      361 atgcgggtaa agaagatgat gagaagattt cagttcgtga gtttattact aagctagatg
      421 aaaaaatgga attgctcaac atgaaagaag aaatggcaga gcgttacctc aacgaaggct
      481 tctctggtgg tgagaaaaaa cgcaatgaaa ttcttcaact tttgatgttg gagccaacat
      541 ttgctctttt ggacgagatt gactcaggtc ttgatattga cgctcttaaa gttgtgtcta
      601 aaggtgtcaa tgccatgcgt ggtgaaggtt ttggtgctat gatcatcact cactaccaac
      661 gtcttttgaa ctatatcaca cctgatgtgg tacacgtgat gatggaaggt cgtgttgtcc
      721 tttctggtgg tccagaattg gctgcgcgtt tggaacgtga aggatacgca aaactagctg
      781 aagaacttgg ctacgactac aaggaagaat tgtaattccc tcgtatcttt taggagaagt
      841 aaatgactag agaaaatatt aaactttttt cagaaatgca cgctgaacca agctggttgg
      901 ctgatctccg tcaaaaagct tttgacaaga ttgagacttt ggaattacca gttattgagc
      961 gtgtcaaatt ccaccgttgg aatctgggtg atggaacgat tacagaaaat gagccatcag
     1021 caaatgttcc agatttcaca gctttagatc atcacttgaa gttggtgcaa gtaggaactc
     1081 aaactgtttt cgaacaaact ccagttgagt tagctgaaca gggtgttgtc ttcacagact
     1141 ttcactcagc tttagaagaa attccagagc tgatcgaaga attcttcatg tcatctgtta
     1201 agtatgatga tgacaagttg gcggcttacc acacagctta ctttaacagt ggtgctgtac
     1261 tctatattcc agataacgta gaaatcacag agccaattga aggaattttc taccaagata
     1321 gcgatagcaa tgtgccgttt aacaagcata ttatgattat cgctggtaaa aattctaaga
     1381 ttagttatct ggagcgttta gagtcacgcg gtgaaggaag tgacaaagta actgccaata
     1441 tcacagtgga agtgattgca cgttctggtg cgcaagtcaa gtttgctgct atcgaccgtc
     1501 taggtgaaaa cgtcactgcc tacattagcc gtcgtggtaa attaggcaac gatgcaagta
     1561 ttgactgggc tatcggtgtc atgaacgaag gaaatgtcgt tgctgatttt gatagtgact
     1621 tgattggtaa tggtagccat gctgacctca aggttgtagc tctttcaagt ggtcgtcagg
     1681 tacaagggat tgatactcgt gtaactaact atggctgcaa ctctatcgga aatatcctac
     1741 aacatggggt tatccttgaa aaagcaactt tgactttcaa tggtatcggc cacatcatca
     1801 agggtgctaa gggagcagat gcgcaacaag agagccgtgt tctcatgctt tcagaccaag
     1861 cgcgttcaga tgctaaccca attcttttga ttgatgaaaa tgatgtcact gcaggacacg
     1921 cagcgtctat cggacaagtt gatccagaag acatgtatta cctcatgagt cgtggattgg
     1981 ataaggcaac tgcagagcgt ttggttgttc gtggtttcct tggatctgtt atcgtggaga
     2041 ttccagtcaa ggaagttcgt gatgaaatga ttgcaactat cgaagagaaa ttgtcaaaac
     2101 gctaagggga agcctatgtt agatgtagaa gcgattcgca aggattttcc aattttagat
     2161 cagattgtca atgatgaacc tctggtctat ctggacaatg ctgcgacgac acaaaaacca
     2221 ctagtagttc tgaaagctat taacagttac tatgagcagg acaatgccaa tgttcaccgt
     2281 ggtgtccata ccttagcgga acgagcgaca gcttcttatg aagctgctcg tgaaaccatt
     2341 cgtaagttta ttaatgcagg ctctacaaag gaagttctct ttaccagagg aacgacaacc
     2401 agccttaact gggtggcacg ctttgctgag gaaattctcg ctgagggaga ccgggtcttg
     2461 atttcagtaa tggaacacca ttctaatatc attccatggc aggaagcttg tcgaaagact
     2521 ggagcagagc ttgtctatgt ctatcttaaa gacggtgcct tggatatgga ggatttgcga
     2581 gctaaattga ctgataaggt taaatttgtt tccctagctc atgcctccaa tgttcttggt
     2641 gtggtcaatc cgatcaagga aatcactcaa ttagcccacc aagttggggc aattatggta
     2701 gtggatggtg ctcaatctac acctcatatg aagattgatg tccaggactt ggatctggac
     2761 tttttcgcct tttcgggtca caagatggct ggtccgactg gtatcggtgt cctttacggc
     2821 aaagaaaagt atcttgagca aatgtctcca gtagaatttg gcggcgagat gattgatttt
     2881 gtctacgagc aatttgctag ttggaaggaa ttgccttgga aatttgaggc tggaacgcca
     2941 aatatggcag gagctattgg acttgcgact gcagttgatt atctggaaaa gattggtatg
     3001 gatgccgttg aagctcatga acaggaattg attgcgtacg tctatccaaa actgcaggca
     3061 attgagggat tgaccattta cggttctcag gatttggctc aacgttcggg tgttattgcc
     3121 tttaacctag gtgatctcca tcctcacgat cttgcgacgg ctctggatta tgaaggagtg
     3181 gctgttcgtg ctggtcacca ttgtgcgcaa cccttgcttc agtatttgga agtcccagca
     3241 acagctcgtg caagttttta tatctacaat accaaggcag attgcgacaa actagtcgat
     3301 gccctacaaa agacaaagga gtttttcaat ggcactttct aaactagata gcctttatat
     3361 ggcagtggta gcagaccatt cgaaaaatcc acatcaccaa gggaagttag aagatgctga
     3421 gcaaaccagt ctcaacaatc cgacttgtgg ggatgtcatc aacctctctg tcaagtttga
     3481 tgcagaggac cgtttggaag atattgcttt tctaaattca ggatgcacga tttcaactgc
     3541 ttctgctagt atgatgacag atgccgtttt aggaaaaacc aaacaagaaa ttttagaact
     3601 ggcgactatt ttttctgaaa tggttcaagg gcaaaaagat gagcgtcaag gccaacttgg
     3661 agacgcggca ttcttgtcag gtgttgccaa attccctcaa agaatcaagt gtgcaaccct
     3721 agcttggaat gcccttaaga aaacaattga aaatcaagaa aaacagtaag acaagtttct
     3781 tttgtcttat gaattattag aaatgaagaa agaaaggata ctatggctga agaaagagta
     3841 gaaccaaaac caattgacct tggtgaatat aaatttggtt tccatgacga tgtagagcct
     3901 gtcttatcga caggaaaagg actcaacgaa ggtgttattc gtgaattatc tgctgctaag
     3961 ggtgagcctg agtggatgtt ggagttccgt ttgaagtctt atgaaacctt caaaaaaatg
     4021 cccatgcaaa cttggggagc agacttgtca gagattgact ttgatgactt aatctactac
     4081 caaaaaccat ctgacaaacc agcccgttct tgggatgatg tacctgaaaa gattaaagaa
     4141 acctttgaac gtatcgggat tccagaagct gaacgtgctt atttagcagg ggcttctgcc
     4201 cagtacgagt cagaagtggt ttaccacaac atgaaggaag agttccaaaa attaggtatt
     4261 atctttacag atacagattc cgcactcaag gaatacccag acttatttaa acaatacttt
     4321 gcgaagttgg taccgccgac agataacaag ttggcagccc tcaactcagc agtatggtcg
     4381 ggtggaactt ttatctacgt gccaaaaggt gtcaaggtag atattccact tcaaacttat
     4441 ttccgtatca ataacgaaaa tataggtcag ttcgaacgta ccttgattat cgttgatgag
     4501 ggagcaagcg tccactacgt agaaggatgt acagcaccaa catattcaag caatagctta
     4561 cacgctgcca ttgtagaaat ttttgctttg gacggagctt atatgcgtta tacaactatc
     4621 caaaactggt ctgataacgt ctataacttg gtaacaaagc gtgctaaggc tcaaaaggat
     4681 gctactgttg agtggattga tggaaacttg ggtgccaaaa cgactatgaa atatccatct
     4741 gtttaccttg atggagaagg agcgcgtggt accatgctct ctatcgcctt tgctaatgca
     4801 gggcaacacc aagacacggg tgctaagatg attcacaatg ctccacatac cagctcgtct
     4861 attgtgtcta aatccatcgc taaaggtgga ggaaaggttg actaccgtgg acaagtcacc
     4921 tttaacaaga actctaagaa atctgtttcc cacattgaat gtgataccat tatcatggat
     4981 gacttgtcag catcagatac tattccattt aatgaaattc acaactcgca agtggctttg
     5041 gaacacgaag ccaaagtatc taagatttca gaagagcaat tgtattatct catgagccgt
     5101 ggattgtcag aatctgaggc aactgaaatg attgtcatgg gatttgtaga accctttaca
     5161 aaagaacttc caatggaata cgcagttgag ctgaaccgct tgattagcta tgaaatggag
     5221 ggatcagttg gataaaattt gattttatac tcttcgaaaa tctcttcaag ccacgtcagc
     5281 atcgccttac cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaacag
     5341 tgttttgagc aacctgcggc tagcttccta gtttgttctt tgattttgag tattagattt
     5401 actcaaaatc aaggattttg aagatgaact tgtatcaaaa aatcgcggtt taaaatcgcg
     5461 attttttata atttctcgtt aacaaagcgg acaaactgat tccaccaaac ttttaagaag
     5521 aaggcttttt caattttctt gtctgctacc atttcgaaac tagggcgctc tgtggtgatg
     5581 taaccttgac caatcaagtc cttgtcttca taagtcaaat ggccaaccac tgttccagct
     5641 tcaagtggtg ctgggattgc tttggaatca ggtgtgaatt gaacagattg ggaagattga
     5701 ttcccaatct gttcgattag atagatatcc tctggagcca ctgcagttac tgtatcttct
     5761 tttccatctt gtacaggggc tttgctatct tgataggcat cgccttgttg aacgattttg
     5821 cgaagtgtaa atgtagaaga aatataatcc attagggaag atgtagctgt aaatcgagcg
     5881 taaggattat tgtcttgatg atctgcattt aaaacaactg tgatgactct catgcctttt
     5941 tcgacagtag taccaacaaa agactctcca gccttatctg ttgttcctgt ttttagccca
     6001 tcaaaaccac cacggtaagc aggcatacct tctaacatgt agttggttga agtgattgtc
     6061 atcccagcaa aagtagaaga aggtttttta gtgatttcta agacttgtgg gtattttttg
     6121 atgaggttgc gagcaacgat agcgacatca taagcactaa gcttattttc ctcatctttt
     6181 ttagaacctg ggtaaatgtt atcccctaga gtttcattgt taagacctgt cgtattgaca
     6241 acagtggcat cctgaattcc ccattccaag agttttgccc gcatcatatc gacgaaatct
     6301 ttttctgagc cagcaatttt ctcagctagg gcaatagcgg cgctgttggc actagatacc
     6361 agagttgctt caagcaactc ttcgacagta taattacggg cctccatagg aatattactg
     6421 gcttcagaat ttgtcgtcaa ttgataagga taatcagaaa tatctacagg agtggagagg
     6481 gtaatacttc cgttttccaa agcttcatag accagataaa cagtaatcaa ttttgttatg
     6541 gaagcaattt cgacaggttg cgttgcatcc ttctcataga gaattttacc agtatttgcc
     6601 tcaacagcaa tcgcatgttt agcggcaatg gtaaaatctt gagcaacagc agtagaagca
     6661 ccccctaaaa gagagacagt taacaaagtt aaaaatattt ttttcatagt agtcttattc
     6721 tatcataaag aaaaaaaata ttcttgcttt aataattcat ctgttaagct ttttgaaaat
     6781 atggtaaaat aaagtgaggg aggtaactca tgtttcgtag aaataaatta tttttttgga
     6841 ccacagaaat tttactctta accatcatct tttacctatg gagacagatg gggtctttga
     6901 ttaacccttt tgttagcgtg cttaatacaa ttatgattcc atttttatta ggaggctttc
     6961 tttattattt gacaaaccct attgttactt tcttaaataa agtctgtaaa ctcaatcgtt
     7021 tgcttggtat tttaattacc ttgtgtactt tggtctgggg aatggtcata ggtgttgtct
     7081 atctcttacc tattttgatt aatcagttat ctagtttgat tatatctagt caaactattt
     7141 atagtcgagt acaagactta atcatagact tatctaatta tcctgcgctc cagaatttgg
     7201 atgtagaagc tacaattcag cagttaaact tatcctatgt ggatattctt caaaatatcc
     7261 taaatagcgt atcaaatagt gtggggagcg tcttgtcagc tcttatcagt actgttttga
     7321 ttttgattat gactccagtt tttttggttt atttcttatt agatggacat aaattcttgc
     7381 ccatgcttga aagaacgatt ctaaagaggg atcgcttgca tattgcaggc ttattaaaga
     7441 atttaaatgc gacgattgct cgctatatta gtggagtttc gattgacgca atcattatag
     7501 gttgtttggc ttatattggc tatagtatta ttggtttaaa atatgcttta gtttttgcca
     7561 ttttttctgg tgtagccaat ttaattcctt atgtggggcc aagtattggt ttgattccta
     7621 tgatcatcgc aaatatattc actgatcccc atagactgct gattgcagtg atttatatgc
     7681 ttgttgttca gcaggtagat ggcaatatct tatatcctcg aatcgtagga agtgttatga
     7741 aggttcatcc aatcacgatt ttagttttac ttttgttgtc aagcaatatc tatggtgtag
     7801 ttggaatgat tgtcgcagtg ccaacctatt ctatcttgaa agaaatttct aagttcttat
     7861 cccgtttgta tgaaaatcat aaaataatga aagaacgaga aagagaatta gctaagtaaa
     7921 agtcaggaga accctgattt ttctttactg gaagtggcct ttagattaga agactgaaaa
     7981 taagttaaag tcttaaacta attttcacag ctaagaatag tagaagttaa tctgataaaa
     8041 atcgaaaaaa ccagtggaat tctgtgtcag ggtaagttcc actggttttc atagtctatt
     8101 aaagttcgaa tgaaacctat ttatagtaga ttgaaactag aatag
//
LOCUS       DAVLTM010000085         8019 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLTM010000085 DAVLTM010000000
VERSION     DAVLTM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.086"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(73..306)
                     /locus_tag="VYN16_001587"
     CDS             complement(73..306)
                     /locus_tag="VYN16_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1404095.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(312..1304)
                     /gene="plsX"
                     /locus_tag="VYN16_001588"
     CDS             complement(312..1304)
                     /gene="plsX"
                     /locus_tag="VYN16_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1404096.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVRLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTFPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1301..2071)
                     /gene="recO"
                     /locus_tag="VYN16_001589"
     CDS             complement(1301..2071)
                     /gene="recO"
                     /locus_tag="VYN16_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1404097.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2068..3237)
                     /locus_tag="VYN16_001590"
     CDS             complement(2068..3237)
                     /locus_tag="VYN16_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1404098.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3386..4396
                     /locus_tag="VYN16_001591"
     CDS             3386..4396
                     /locus_tag="VYN16_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1404099.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4646..4726)
                     /locus_tag="VYN16_001592"
                     /pseudo
     CDS             complement(4646..4726)
                     /locus_tag="VYN16_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4761..5198)
                     /locus_tag="VYN16_001593"
     CDS             complement(4761..5198)
                     /locus_tag="VYN16_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1404100.1"
                     /translation="MSQEFINPSDGVIRRYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLLDVARDFLKADA
                     KIFWAQLGLESLEAEEILRAGGCDDIVMNRCIKREHTRLIGEA"
     gene            complement(5283..7916)
                     /gene="polA"
                     /locus_tag="VYN16_001594"
     CDS             complement(5283..7916)
                     /gene="polA"
                     /locus_tag="VYN16_001594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1404101.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2206 a         2073 c         1480 g         2260 t
ORIGIN      
        1 gattttacca gcttgtccga tatttctcat ttctaaaaac catctacttc cgttgacttg
       61 gaactccgat tgctattttc cttgaatgat tttaaccaca tctcctacgt tttggagttg
      121 gtcaatttct tcatcgctga tttcgatact aaattcatct tccagagtca agataaactc
      181 catcaagtca acagaatccg catccaaatc gtctttcaga ctcaaggatt ctgtcacgac
      241 aaagtcctct ccctgtcgct cttggataat ggtcacaata cggtcaaaaa tttctttttc
      301 tgtcatctct tttattctcc tgaaaattca cgcgcagtct gggcaaccac gtctgtttct
      361 agcatggtac ggatctgacg aatcgtacta taaacagcct tggcatcgct tgagccatga
      421 gtcttgacaa caggtgcctt aacaccaaac aagaccgctc caccaacatc tgaataattg
      481 agctgttttt tcaaaccact gaggctgtcc ttgagaagga gggcacctag tttcgctcga
      541 agaccaccac ctgtaatagc tgtcttgagc aagcccatga ttcccatagc tgtcccttcg
      601 atggatttga gcacagcgtt tcccgtgaaa ccatctgcca caacaacatc tgcaacgcca
      661 ttcatcaaat cacgcgcttc cacgtttccg ataaagttca aactttcatc agccgccagt
      721 aattcataag tttccttacg aagcgggtcg cccttgctac tctctgttcc gttgttgagc
      781 aaaccaacgc gtggttgcgc aatgccacgg acatttttag catagaagga acctagaacc
      841 gcatattgat ggaggtgctg ggctgtattt tctgcattgg caccaaggtc tagcatgtca
      901 aaaccttttc catcaacggt aggaaaggta gacatgagtc caggacggtc gatattcttg
      961 atacgaccca cgatgaagaa tcctgctgcc aacaaggcac ctgtattccc agccgaaagg
     1021 acagcgtctg cttcaccatc tttgacagcc ttggctgcca ataccatact ggcatttttc
     1081 ttattcctaa tagctctcgt aggttcatca tccgaatcaa tcttctcatc cgtatggata
     1141 atgctgacgc gctctgtcgc tgtcagatat tgcttgattt tagcttcatc tccgtaaagt
     1201 cgaacctcga tatctgaaaa gtcagatagg gcttgattga caccctcaac aatggcctga
     1261 ggtgcgtaat cgccccccat ggcatctact gcgatttttt tcatttcttt tcctctttta
     1321 gtaattgtcc ccagtctgct agggaatcaa taaatttctt tgattttagg tgaatcccaa
     1381 cgtactcttc atataattga tccataaatt ggcgtagctc ttgcttgatt ccaggcttga
     1441 gcgaaatggt ctccaaagtc tcaaaatcaa tagcttgaaa ttgattgagc agatagggga
     1501 tattgggatt gagatgacaa cgtctcttat cctcatgata atgctctgga cagaggcagg
     1561 ctccatattt gaaagaaaag tcaaaagcct gaccaacccg atggcagaag acacactcat
     1621 taaaattgag gctgattcca aatcgagtca aaatttgaat ttcaaaaata ttggtcaaaa
     1681 cctgataatc caagcctgct tccatcaact ccaaagtctt ttgcaaaaaa gcaaacaagg
     1741 gagcatcctg ctgattgtcc tgcaaactag catctgcaag agctgccaca taggtcgcat
     1801 aggccatgac aaagaggtca ctattaatct tgggaaaagt catgacctca tgatagtctt
     1861 cgatgtaact gagtccgtca tcattgattc gcaagagaaa tcgtgccagc accaagggct
     1921 gaataacagg cgccagctta gactgaccag cgtgtttgac aaaaaacatg cgtttgccaa
     1981 cctgctctgt aaaaattttg acgagcttgt catcctcacg aaaattgcga ttgtaaagca
     2041 ccaagccttg actcgtgata gactgaatca tgcttctctc atgtactcct caagtcgttt
     2101 catggcttct ttgatagtct ccatgctggc tgcataagat aggcggacgt agccttcccc
     2161 gtaacgtcca aaggctgcac cagggataaa ggcaacggcc ttcttctgag caaaatcctt
     2221 cagaaaagca aaggagtctt gattgtagcc cgctggaatt ttagcaaaaa tatagaaggc
     2281 accgtctggt ttgataatct caaaaccaag agcagtcatt ttttcgatga tatagtcccg
     2341 acgctggata tattccttct tcatgggctc cgcatcattt ttaccagccg tcaaggcttc
     2401 taccgcagca tgttgcgcca tggtatttgc ggcagtgacc aagtattggt gactcttgat
     2461 taactgggct gtgaaggccg caggagcgaa aatcagcccc aaacgccaac ctgtcatggc
     2521 atgcgattta gacaaaccat tgataataat agcctggtct ctcaacatcg ttcctagaga
     2581 cacatgggct tcgcctgtgt aggtcaattc tgagtaaacc tcatcacaga caacaaaaat
     2641 ttcgtacttg cgtaaaacag ctgccaaggc ctctaactgc tctcgactgt aggtaattcc
     2701 tgtcggattg gctggatagt tgagaataac cgccttgagc ttatcaccct gctccaaaat
     2761 ggccttctcc aacatctcag gagtcaagac aaaaccattt tcagtcgtat caatctcaac
     2821 aatttctgcc ccaactaagt taacaatcgg ttcatagcct ggataagcag gagctggcaa
     2881 aagtaccttg tctccctctt ccaaaatagc cgtcaaagtc gcagataaag cctctgtcgc
     2941 cccaattgta accaagattt cattttcagg agcatagtcc agttggtact tttccttaac
     3001 aaagtcacta gctgcctgac gtagagtcag cagaccactc atccctgtat agtaggattg
     3061 gttctgatca atcgctcgct tggccgcctc cttgacatgg tctggcgttg taaaatcagg
     3121 ttcccccaag gtcaaacgca agaccccagg aatctccgaa atagcctggt caaactgacg
     3181 aatcaacgaa acttgaattt tatctaactg tttattaaag cgcttagtta agtccataga
     3241 tacctcctac tttcaaaacg tatctctatt atactacaaa agccccccga ccgcaaaaat
     3301 acattagaga atagctttcc actttctatt ttacttttcc tctttacagg tctataatgg
     3361 taacagattc tatttggagg tttttatgtc atttctatca aaaaatggag caggcatctt
     3421 ggtctgcctt ctcatttcca tcctatcttg gtacttagga ggattcttcc ctgttattgg
     3481 cgcacccgtt tttgcgattt ttatgggaat gctcctacat ccctttctct cgtcttataa
     3541 acaactggat gttggattga cctttagttc caagaaattg ctccagtatg ccgttatctt
     3601 acttggtttt ggtctcaata tctctcaagt cttcgcagtt gggcaatctt cactccctgt
     3661 catcctttcc accatttcaa tagccttgat tgttgcctac ctcttccagc gcttctttgc
     3721 cctggataca aaactggcta ccttgattgg agtaggttct tctatctgtg ggggttctgc
     3781 cattgcagcg acagcgcctg ttatccatgc taaggaaaag gaagtagccc aagccatctc
     3841 cgttatcttt ttcttcaatg tcttggctgc gctcatcttt ccaaccctcg gcacctggct
     3901 tcatctatcc aatgacggct tcgccctctt tgcaggaact gcggtcaatg atacttcctc
     3961 tgtaaccgcc acagctagcg cttgggacag tctctaccag accaataccc tcgaatccgc
     4021 aaccattgtc aaactcacgc gcaccctagc tatcatcccc atcactctct ttctctccta
     4081 ctggcaaagt cgcgagcaaa aaaataagca aggcttacaa ctgaaaaaag tcttcccact
     4141 ttttatcctt tacttcatcc ttgcatcgct cctaaccacc cttctgacct ctctcggtgt
     4201 gtctagtagt ttctttaccc ctctcaaaca actctctaaa ttcctcatta tcatggccat
     4261 gagtgccatc ggtctcaaaa ctaatctggt cgctatggtc aaatccagtg gaaaatccat
     4321 tgttctcgga gccgtctgct ggattgccat catcctcacc agccttggta tgcagaccct
     4381 tatcggcatt ttctaatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc
     4441 gtaggtatat gttactgact tcgtcagttc tatctgcaac ctcaaaacgg tgttttgagc
     4501 tgacttcgtc agttctatct gcaacctcaa aacggtgttt tgagctgact tcgtcagttc
     4561 tatctgcaac ctcaaaacgg tgttttgagc tgacttcgtc agtcgtatct acaacctcaa
     4621 aacagtgttt tgagctgact tcgtcagtcg tatctacaac ctcaaaacag tgttttgagc
     4681 aacccgcagc tagtttccta gtttgctctt tgattttcat ttagtataac acaaaaggta
     4741 gcccatcagc tacctttttc ttatgcttcc ccaatcaagc gtgtatgttc tctcttgatg
     4801 caacgattca tcacgatgtc atcgcatcca ccagcacgca agatttcttc cgcctctaga
     4861 ctttcaagtc ccagctgcgc ccaaaaaatc ttggcatcag ccttgagaaa atcacgcgcc
     4921 acatcgagca gaaattcact gcgacgataa acattgacaa tatctacagg aaaaggaatt
     4981 tcagcgaggc tagcataagc cttttcaccc aagatttcgc cacctgccgc cttgggattg
     5041 actgggatga ttttatagcc ccgagcctgc atttcctttg tcactcgatt gctggttgtt
     5101 tcttcacggt cagacaaacc caccacagca agggttttac tcgttgcgag ataccgacga
     5161 atcacgccat cacttggatt gataaattct tgactcatag aaatcctcct ttttcatcag
     5221 tatagcatat tttgaaaagg tttgcagaat tctactacaa aaaaggagga ctagccccct
     5281 ttttatttag cctcgtacca ggttgcccct tcattctcat ctgcgataag aggaacactg
     5341 agttgaatgg cttcttccat ggtttgtttc accaattttt tcatctctac caattcagat
     5401 ttaggcactt caaggacgat ttcatcgtgc acttgtaaca gcatcttagt ctgataacca
     5461 cctgcaacca aggctttatc cagctgaatc atggcaatct tgagaatatc tgctgccgaa
     5521 ccctggatag gtgagttgat agcagttcgc tccgcaaaac cacgaatatt gaagttgcgc
     5581 gaattgatat ctggcaactc acggcgacgc ttaaagaggg tctctacata gctcttatca
     5641 cgcgcctccc gcaccacttc atccatgtag tttttaatac ctggaaaacg ttcaaagtag
     5701 gtatcaatgt aggctttggc ttccttacga ctaattccca aattattaga caagccaaag
     5761 tctgaaatcc cataaaccac tccaaagtta actgccttgg cattgcgacg gtcgtttgca
     5821 gtcacatcat caggacgctc aatgccaaag acccgcatgg ctgtcgaagt atggatatct
     5881 gccccctctt ggaaggcctt aatcaagtgc tcatccttag aaatatgcgc caaaacgcgc
     5941 aattcaatct gtgaatagtc agagctgagt agcacactat cctcccactc tggcacaaaa
     6001 gccttccgaa tcaagcgccc ctgttccaat cgggcaggaa tattttgcaa gtttggatcc
     6061 acactagaca aacgcccggt ctgggtcaaa tcctgcacat agcgagtatg aatctttcca
     6121 tcagccaaaa tccagtcctg caagccaatt acataagtag attgaatctt agcaatttga
     6181 cggtaatcca ggattttctt aacaatcgga gcaataggag cgagacgctc caacacatcc
     6241 actgctgtcg aataacctgt cttggttttc ttagtgtatt ctagaggaag tcccaatttc
     6301 tcaaagagaa gcacgcccaa ctgcttaggc gagttgacat taaactcctc accagccagc
     6361 tcgtaaatct cttgagtcag tttttcaatg acaagctcat tttcagcctg catctcaagc
     6421 aaggtctctt tcttgaccat aatcccagca atttccatct tggcaaggac aaaagccaga
     6481 ggttgctcca tatcataaag aagctctaat tgcccatttt cgctgagttt ttcaagtaaa
     6541 ataggctctg tttctaccaa aacagcaagt ttacaagcta agtgttccaa gaatttctca
     6601 cgttcaggaa tggccttttt aacaccctta ccgtagaaag tttcatcatc aaccaagtaa
     6661 gtctgaccat aaagactagc gatggtcgca atttcattgt cctccacagt cgaaaggagg
     6721 tatttagcca aacggatgtc aaaagcaggc gcctgcaaat ccacaccaaa acgttgcaaa
     6781 agaactttaa ccttcttaaa gtcataaact ctcagagatg ttttttctaa gaaatccttg
     6841 aaaatcgggt cttgcaacag ctcaagcttg tctgtggcat agagcttatc ctcacaagac
     6901 caggcaaatc caaccaaatt atccgtatgg taattctcac caaaaagctc aaagtggaag
     6961 atagactctt cactcagcat atcttgactg atttggtcaa caatagtaaa atctaaaccc
     7021 ttagccacat cagctgacga cacatttaaa gcctgcttga gctgtttgaa gcccatctca
     7081 tcgtaaaatt tcccaagatt ttcaacatct ggaccactat agaccaagtc ctctaaacca
     7141 atcgcaatcg gtgccttggt atcaatggtc gctagtgttt tagacaaaaa ggcctgttcc
     7201 ttgtcattga tgagattttc cttcatctta gaagtcttca ttccatcaat attttcataa
     7261 atcccctcaa gcgaaccatg ctccagcaag agcttaatac ccgtcttttc accgactttg
     7321 gtcaccccag ggatattatc cgacttatca cccatgagcg ccttgagatc gataaactga
     7381 gctggtgtga ggcccatttc ttccatgagg taatctggcg taaaggcctc aaactcagcc
     7441 acacctttct tggaaatttc aaccaccgta tgctcatccg tcagctgaat caaatccttg
     7501 tccccactga caatggtaat atcaaaacca tcctgctctg ctagcttatc cagcgtccca
     7561 atgatgtcat ccgcctcata ctgagccagc tcatagtgac gaatccccat atgatccagc
     7621 aactcacgaa tgaaaggaaa ttgctcacga aactcatcag gagtcttggc ccgaccaccc
     7681 ttatagtccg catacatctc tgtccggaag gtcgtctttc ccgcatcaaa agccaccaaa
     7741 atatgactcg gctcaacccg ctccaacaaa tgactcaaca tcaactggaa accataaatc
     7801 gcattggtat gcaaaccagc cgcattctta aaacggtcca actgctgata cagcgcaaaa
     7861 aacgcccgaa aagctacaga agacccatca atcaataata attttttctt atccatacac
     7921 ccattataaa ggaaagaatc aaaaaatacc attgggaaga gctagagcaa gtatttttca
     7981 aactttttcc gaataaatag atagagccag agaatctag
//
LOCUS       DAVLTM010000086         7943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLTM010000086 DAVLTM010000000
VERSION     DAVLTM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.087"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..920)
                     /locus_tag="VYN16_001595"
     CDS             complement(<1..920)
                     /locus_tag="VYN16_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1404102.1"
                     /translation="MMRSTKELQHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEI"
     gene            complement(917..1774)
                     /locus_tag="VYN16_001596"
     CDS             complement(917..1774)
                     /locus_tag="VYN16_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1404103.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRIKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(1778..3124)
                     /locus_tag="VYN16_001597"
     CDS             complement(1778..3124)
                     /locus_tag="VYN16_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1404104.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            complement(3137..3421)
                     /locus_tag="VYN16_001598"
     CDS             complement(3137..3421)
                     /locus_tag="VYN16_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1404105.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(3425..5455)
                     /locus_tag="VYN16_001599"
     CDS             complement(3425..5455)
                     /locus_tag="VYN16_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1404106.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            5646..6653
                     /locus_tag="VYN16_001600"
     CDS             5646..6653
                     /locus_tag="VYN16_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1404107.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            6656..6856
                     /locus_tag="VYN16_001601"
     CDS             6656..6856
                     /locus_tag="VYN16_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1404108.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            7008..7190
                     /gene="rpmF"
                     /locus_tag="VYN16_001602"
     CDS             7008..7190
                     /gene="rpmF"
                     /locus_tag="VYN16_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1404109.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            7206..7355
                     /gene="rpmG"
                     /locus_tag="VYN16_001603"
     CDS             7206..7355
                     /gene="rpmG"
                     /locus_tag="VYN16_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1404110.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(7834..>7943)
                     /locus_tag="VYN16_001604"
     CDS             complement(7834..>7943)
                     /locus_tag="VYN16_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1404111.1"
                     /translation="TVSGQWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         2665 a         1620 c         1195 g         2463 t
ORIGIN      
        1 taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc attaagaaga
       61 taatccattt tccctacttg accaaatctt tcttgaacac ccatccgatg aatttttgtt
      121 attccatcat cagagaataa ttcacataaa gcactgccaa ttccacctat ctgattgtgg
      181 ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc tggtatcggt
      241 ttgattctaa ataaatctat cacacctact gaataaccta atttagacag ttcatctgca
      301 actcgaatac ttggagcaac cattatacca gaagcaacga ttacaagatc ttcaccatgc
      361 cttaactcaa tgtagccttt agaaaaatct tctccacctt gatacacagg aactggagct
      421 tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt caatatttca
      481 cgaaattgga tatcatcagt tgcttcaaaa atgatcgatt taggaattaa acgtaacaat
      541 ccaatttctt caaatggcat atgtgtccca ccattcattt ctgcagttac tcctgcatct
      601 gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa ttgatcaaat
      661 actcttcgtg aagcaaaagg accaaatgta tgaagataag gtctaaaccc ctgaatagac
      721 aagcctgctg caaggccgac catttctgct tccataatcc caacattcac ataacggtct
      781 ccaaagtcct tttcaagatt atgagtagcc atcgaacttg acagatcggc ttctaagact
      841 actatatcag aataactttg attagcctct aaaaggaagt ctctatatac atgctgtaat
      901 tctttcgtac ttctcatcat tctgtttcct ccaattcctg acttaatctt tctacaactg
      961 aagttaacat ttgtctctcc tctacagtag ggcgaagatg atgattggat ttcatttctt
     1021 ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta ggtgatgaat
     1081 tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct gaacccttga
     1141 tcctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct ggattacaaa
     1201 tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca attaagttgg
     1261 ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca tttaactcac
     1321 catctccaac aatagcataa gtataaaagg gactctttct tattctctga ccatatgcaa
     1381 gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct atgcctggcg
     1441 ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa gaatataaga
     1501 attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt cctccgtgac
     1561 cttttgataa tatgaaataa tctctatctc gtgctgcaaa tatttctgga gtcattggca
     1621 ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct ccgtaatgtc
     1681 cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagtc gcaaattttc
     1741 ttaactcatc ttccctattt ttacttaaaa tcatccctta ttcctccttt gcagatggct
     1801 ttgtaataaa ggatactcca aacataactg ctagaataag aacaagacca atcacaatgc
     1861 ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga ccaaaatctg
     1921 catctgagaa agttgatcct tggaaaccaa gtcctcccaa aactggcatt aaaaagactg
     1981 gaagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca cgaacaccac
     2041 cagatgcatt cccaatgaca cctgcagtcg ctccacagaa gaaatgaggc acaacacctg
     2101 gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa ccaccaacaa
     2161 aactagagat aaatccaatt agaactgcat tgggtgcata agtataaaca atcggacaat
     2221 ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag gctggaacaa
     2281 tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct gcaaattgac
     2341 ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct ttttctatat
     2401 attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatggat aaagtgatac
     2461 taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc tctgttgatt
     2521 ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc aaagaactga
     2581 aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt tgaacaaatg
     2641 ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagata agaggcaagc
     2701 tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat agagtgtggt
     2761 gccctgttaa aaaaatatat ttaaatcgag taaaacgagc gattaagata ttgaacacca
     2821 tgcctgcaaa cataatcagt gcagtagctg agccatatgt tgttaaagct acagctacaa
     2881 ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac atggtaccaa
     2941 atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag aaaccaacaa
     3001 aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga actaatccta
     3061 aaattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat atgaacttca
     3121 tcataacgct agcctcctat ataagtcctt tttcttcaca aagtttagta attaattctc
     3181 gtagttcatc catatcaata atactattta agatacgaac atctccaaga tgactagctg
     3241 aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct gctccaccta
     3301 aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca gattcaatat
     3361 tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca atttttaaca
     3421 ttatctaatc ctcctgttta attatcattt taatgtcatc atagtttttt gatgatatta
     3481 aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa gcctttaaat
     3541 gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt tctggattat
     3601 ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca ttcacacctt
     3661 catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa ggcccaaact
     3721 cttctacttt ttgaatcatt gcctcagggt agttctcagt tatcttatct tgatccaaaa
     3781 gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa ctaacctgat
     3841 aggtttcttt ggtaatgagt tgttctagca ctggtacaat ttcctttcta tcattttttt
     3901 ggtaaagata attctttaac gccaatctta attccaattc ttgtgtaata attccatatc
     3961 ttttgacaat attcaggatt tgttcaatct caaaatcccc atactctaaa ttcggaaaat
     4021 cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata accgaaacta
     4081 gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca tagtcactac
     4141 tagctttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt tgaggaaata
     4201 atgctaatag attctctttt aacatcaatg aagaactaac accattagga caaatgattg
     4261 ctgctttata gcatttttga ggcaaagtat ctgctttctt taaataacct ccgaaatgga
     4321 taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc atcaagggca
     4381 tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca tgcaatacat
     4441 attcatttga actaggtagg ccgaacttta atctatagta agccggtata aggtggcggc
     4501 gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata tcttccattc
     4561 tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct acttcacctt
     4621 caaagcaact tgataataaa acggtgatat agcgataatc atcctcagaa aacaccgtat
     4681 ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct tgaataacag
     4741 gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac ctacacagaa
     4801 tgaatactaa accgaaaagg caaactttta attgattaac aataggtact agctgtagct
     4861 tctcataata atctttaact acctgattta tcaaatcata agttaatgaa tacccccaac
     4921 tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac tcactaacca
     4981 tttgaaaagc taagcggtgc ttattccact ctgaaccgtg taaagtataa ccttttgctc
     5041 tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat tgaatatcag
     5101 ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc gatataaaat
     5161 ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt ggtaaaacca
     5221 attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat aaactatagc
     5281 gaccttgttt tttatccagc actatccctt tattagctag ataaggcact aaataatcta
     5341 ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca tataatgata
     5401 gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca aacatttctc
     5461 ttcctttcta actacaatta tagtgtaaaa attataagaa taaaagagta tttgagcaca
     5521 atataatctc accttttaaa agaaattatt gggctgaaac tttttagtga ttattttcaa
     5581 ctttctattg acttttaaaa acaaagtgat atcatttaga tatcaaataa taggaggtca
     5641 ttcttatgac tgaaaaactc atcaattcaa aaccaaatgg tgtattcgca ttgattctca
     5701 ttgaattgac aatcgtactt ggtatcttta tatttataat gggtgttggt tcggaaaaca
     5761 tttttggaat tattatcgga cctttactaa tcgtaattgc aggtctagct catgctggtt
     5821 taaaagttgt caaacctcag gaagctctgg ttctgacact ctttggtaat tatacaggta
     5881 ccatcaaaga acctggtttt tactttgtca atcccttcag cgtagcagtc aaccctgcaa
     5941 accacactcg acttggacaa agtggtgatg ttagcacaaa atctcctttt ttaggagcta
     6001 aatcatcaaa tgacaatgat gtaaatcttg aaattggcaa gaaacagatt tccctcaaag
     6061 tcatgacctt gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac cctgtagaaa
     6121 tcggtatcgc ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc aacgttgata
     6181 actacaaaga atatctttca ttgcaatgtg atagcgccct ccgtaatatt gtccgcatct
     6241 atccttacga tgtgtctcct aatgtggata ctacgggtga cgggcaagca gatgaaggta
     6301 gtctccgtgg ctctagcgaa attgttgcta accgtattcg tgaagaaatc caaagtcgtg
     6361 ttgaggatgc tggcttggaa atccttgaag cacgtatcac ttacctagct tatgctccag
     6421 aaattgctgc cgttatgctt caacgccaac aagcatctgc cattattgat gcacgtaaga
     6481 tgattgtaga tggtgctgta ggaatggttg aaatggcact agaacggctc aatgaagggg
     6541 aattggtaga acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc cttgtcgttc
     6601 tttgtggcaa tcatgatgca caaccaattg tcaacacagg aagtctttac taaagatggc
     6661 tgaagctaaa aaaaagcaga tcccccttcg actctcaaca aagttatacg ctgcactcgc
     6721 atcatgggca gaagacgact ttcgttcagt caatgggcaa atagaatatc tcctcacaga
     6781 gtgtgtcaag caacggaaaa aagacggaaa atacgtatca gaaaccattg acgaaccatt
     6841 tgagattgat atttaacacc ttctcctgtc tgccgtgtcg gataggagaa ttttttcatc
     6901 tttttttgtc aagtaacttt actttacaaa aaaaatgtgt tatcctagta tggttgatga
     6961 aaatcagtag attgaatcga atataaatac ctaaaggaga aatcaaaatg gcagtacctg
     7021 cacgtcgcac ttcaaaagcg aagaaaaaca aacgtcgtac acactacaaa gtaacagctc
     7081 catctgtaaa ctttgacgaa actactggag attactcacg ttctcaccgt gtatcactta
     7141 aaggatacta caaaggacgt aaaatcgcta aagctgcatc agctgaataa tagaagggag
     7201 atactatgcg cgtaaatatt acacttgaac acaaagaatc tggtgaacgc ttgtacctta
     7261 cttctaaaaa caaacgtaac actccagacc gccttcaatt gaagaaatac tcaccaaaac
     7321 ttcgcaaaca cgttgtgttt acagaagtga agtaagcatt caatacgaat caatacagaa
     7381 gaaaaacgct tgatcagcgt tttttctttt tgttaatttc actatattgc aagggaaatc
     7441 attcgaatga ctacattatt gaaataagaa tacggattcg gacaattaaa agcaagagga
     7501 actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc tctacactat
     7561 ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga atatgtaagt
     7621 gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat acaaaagttg
     7681 accaactaga tttctgaata tccttatata tccattctta aaattggttt aaatagcgta
     7741 gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag aagatttttt
     7801 agttcagaat gattagattt agctaatgga tacctatcct acccactcac cgttagcatt
     7861 gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat tataggtata
     7921 gtaccattga ccagaaactg taa
//
LOCUS       DAVLTM010000087         7849 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLTM010000087 DAVLTM010000000
VERSION     DAVLTM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.088"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..544
                     /locus_tag="VYN16_001605"
     CDS             <1..544
                     /locus_tag="VYN16_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1404112.1"
                     /translation="AYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHTMVQRIV
                     KPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGAGIAGVA
                     IGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQLRSEEQ
                     ALHFVPNRNITVVSNFSRTD"
     gene            601..1509
                     /locus_tag="VYN16_001606"
     CDS             601..1509
                     /locus_tag="VYN16_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1404113.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1461..2051
                     /locus_tag="VYN16_001607"
     CDS             1461..2051
                     /locus_tag="VYN16_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1404114.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYALTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2054..3160
                     /gene="yqeH"
                     /locus_tag="VYN16_001608"
     CDS             2054..3160
                     /gene="yqeH"
                     /locus_tag="VYN16_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1404115.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKNKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            3374..3685
                     /gene="yhbY"
                     /locus_tag="VYN16_001609"
     CDS             3374..3685
                     /gene="yhbY"
                     /locus_tag="VYN16_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1404116.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3737..4366
                     /locus_tag="VYN16_001610"
     CDS             3737..4366
                     /locus_tag="VYN16_001610"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1404117.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4366..4959
                     /gene="yqeK"
                     /locus_tag="VYN16_001611"
     CDS             4366..4959
                     /gene="yqeK"
                     /locus_tag="VYN16_001611"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1404118.1"
                     /translation="MSYQDYINCSREALLERMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            4960..5460
                     /locus_tag="VYN16_001612"
     CDS             4960..5460
                     /locus_tag="VYN16_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1404119.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5473..5826
                     /gene="rsfS"
                     /locus_tag="VYN16_001613"
     CDS             5473..5826
                     /gene="rsfS"
                     /locus_tag="VYN16_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1404120.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            5929..6675
                     /locus_tag="VYN16_001614"
     CDS             5929..6675
                     /locus_tag="VYN16_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1404121.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFSMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6685..7782
                     /locus_tag="VYN16_001615"
     CDS             6685..7782
                     /locus_tag="VYN16_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1404122.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         2331 a         1563 c         1810 g         2145 t
ORIGIN      
        1 ggcttatatt gaaaagctag atgtgacaac cattatcgag aatattctaa ccaaggtcat
       61 ttctctttta ctgcttttga ttgtatttta tattgctaaa aaaatgcttc ataccatggt
      121 gcagagaatt gtcaaacctt ctctaaaaat gtctcgtcat gatgtcggac gccaaaaaac
      181 catctcacgt ttactagaaa atgtgtttaa ttatacgcta tatttctttt tactctactg
      241 cattttgtcg attttaggtt tgccagtttc tagtttgctg gctggagctg gtattgctgg
      301 ggtagcgatt ggtatgggag cccaaggctt tctgtctgat gtcatcaatg gctttttcat
      361 cctctttgaa cgtcaactgg atgtgggaga tgaggtcgtt ctgacaaatg gaccgattac
      421 tgtatcgggt aaggttgtca gtgtgggaat tcgtacgaca cagcttcgta gcgaggagca
      481 agcccttcac tttgtcccta accgaaatat cacagttgtt agcaatttct cacgcacaga
      541 ctagacctgt tattttaagt aatttgtggt acaatagagg gagtttaata aggagaaaag
      601 atggttttag aaaagcagtt gggcaatggt tgtacctgga tagacctaga cctaggaaag
      661 ttgaataaac tagaagacct ttctgaaatt tacggtttgg acaaggaaac cattgaatac
      721 gcactggata gaaacgagcg cgcccacatg gattaccacc gtgaaagtgg gacggttacc
      781 tttatctata atgtcctaga cgtaaaaaag gacaaggcct actatgagac ttttcccatg
      841 acctttattg tcgagcatcg tcgcctgatt accattagta ataccaagaa cgcctatgtc
      901 attgaacaga tgactcgtta tctggagagc catgacacgc tttcgattta taagtttctc
      961 tttgccagtc tggaaattat cagcaatgcc tactatcctg tcattgagca gatggacaag
     1021 agtagggatg aggtcaatga cctcttgcgc cagcgaacta ccaagaaaaa cctctttgcc
     1081 ctgtctgatt tggagactgg tatggtttat ctgacggcag ctgccaaaca aaatcggatt
     1141 ttgttagagc atattcaagg tcatgccttg tatcgtagtt ttgatgagat tgagagagaa
     1201 cagtttgatg atgccatgat tgaggctcat cagctggtat ccatgacaga cctaatctct
     1261 cagattttac agcagctttc agcctcttac aacaatattc taaacaataa tctgaatgac
     1321 aatttgacaa ccttgactat catttcagtc ttgctagctg ttttggcagt cgtgacaggc
     1381 tttttcggaa tgaatgttcc cttaccttta acagatgagc cccatgcttg gctctatatc
     1441 agtttggcta gtgcaggttt gtggattgtt ttatccttgt tactaaggaa aattgcgaaa
     1501 aaaagttaag aaaaggagcc agaatggcga ttgaaaatta tataccagat tttgctgtgg
     1561 aagcagtcta tgctctgaca gtcccaagcc tgcaggcgca gggaatcaag gctgttttgg
     1621 tcgatttgga taataccctc attgcttgga acaaccctga tggaacgcca gagatgaagc
     1681 aatggctaca tgaccttcgg gacgcgggta ttggcattat cgtagtgtca aataacacca
     1741 aaaaacgcgt tcaacgagca gttgagaaat ttgggattga ttacgtttac tgggccttga
     1801 agcccttcac atttggtatt gaccgtgcta tgaaggaatt ccactatgac aaaaaggaag
     1861 tggtcatggt tggcgaccag ctcatgacag atatacgagc agcccaccgt gcagggattc
     1921 ggtcaatttt agtcaaaccc ttggtccaac atgactcaat caaaacgcag attaaccgaa
     1981 ctcgtgagcg tcgtgttatg agaaaaatca ctgaaaagta cggaccgatt acatataaaa
     2041 aaggaattta actatggaag aaattctctg tattggttgt ggagcaacca ttcagacgac
     2101 agataaagct ggtcttggtt ttacccccca gtcggcactt gaaaaaggtt tggagactgg
     2161 cgaagtctat tgccaacgct gtttccgtct ccgccactac aatgaaatca cagatgtcca
     2221 gttgacgaac gatgatttcc tcaagctctt gcacgaggtg ggagacagtg atgccttagt
     2281 ggtcaatgtc attgatatct ttgattttaa tggatctgtc atcccaggtt taccacgttt
     2341 cgtctcgggc aatgatgtcc tcttggtagg aaataaaaaa gatatccttc ctaagtcagt
     2401 taagtctggt aagattagcc agtggctcat gaaacgtgcc catgaagaag gtcttcgtcc
     2461 agtcgatgtg gtcctaactt cagcacaaaa taaacatgcc attaaggaag tcattgacaa
     2521 gattgaacac taccgtaagg gccgcgatgt ctatgtggtc ggtgtgacca acgttggaaa
     2581 atcaactcta atcaatgcta ttatccaaga aatcacgggt gatcagaatg tcatcactac
     2641 ttcacgcttc ccaggtacaa ccttggacaa aatagagatt ccgcttgacg acggatctta
     2701 tatttacgat acgccgggaa ttatccaccg ccaccagatg gctcactact tgacggccaa
     2761 aaacctcaag tatgtcagtc ctaaaaagga aatcaagcct aagacctatc agcttaatcc
     2821 tgagcaaacc ctatttttag gtggtttggg acgctttgac tttatagcag gagaaaagca
     2881 aggatttact gctttctttg ataatgaact caaacttcat cgtagcaagc ttgaaggagc
     2941 tagtgctttc tacgataagc acctgggaac tcttctgaca ccaccaaata gcaaggaaaa
     3001 agaagatttc ccaaggctag tccagcatgt ctttaccatt aaaaataaga cagacctagt
     3061 catctcaggc ctaggttgga ttcgtgtaac aggcatagca aaagtcgccg tctgggcacc
     3121 agaaggcgtc gccgtcgtca cacgaaaagc aattatttaa aacaaaatcg ctgagcgaac
     3181 aaagtgagct catatagcga tagttattgc cgtggtgtga ttgccaatct gtagattggc
     3241 aaggggcaca attgagacca aggctcaatt gtgaggtcag gaaatccatt tttcaaagat
     3301 gaaatctttg gaaaattagt tccgaccgtc cctcaccacc taaaataact atccaaaaac
     3361 aaggaagaaa attatgtcat taacatcaaa acaacgtgcc ttcctcaaca gccaggcaca
     3421 caccctcaaa cctatcatcc aaatcgggaa aaatggactc aacgaccaaa tcaaaaccag
     3481 cgtccgtcaa gctcttgatg cgcgtgaatt aatcaaggtt actctcttac aaaacacaga
     3541 tgaaaacatc cacgaagtag ctgaaatttt ggaagaagaa atcggtgtgg atacagtcca
     3601 aaaaatagga cgcatcttga ttttgtttaa acaatctagc aagaaagaaa atcgcaagat
     3661 ttctaagaaa gtcaaagaaa tctaagaccg aaactccaaa taactgtttt tatagagaaa
     3721 taaaggggac tagcctatga caatcgaact attgactccc tttaccaagg tagagttgga
     3781 gccagaaatc aaggagaaaa aacgcaaaca agttgggatt ttagggggga attttaaccc
     3841 tgttcacaat gcccatctca ttgttgcgga tcaagtacgg caacagttgg gactggatca
     3901 agttctgctc atgcctgaat accaacctcc tcacgttgat aaaaaggaaa ccatccctga
     3961 acaccatcgt ctcaagatgc ttgagttggc aattgaggga attgacggcc tagtcattga
     4021 aaccattgag ttggagcgca agggtatttc ctacacctac gacaccatga agattttgac
     4081 agagaagaat ccagatacgg attattactt tatcatcggc gccgacatgg ttgactatct
     4141 gcctaagtgg taccgaattg atgaactggt tgacatggtt cagtttgtgg gggttcagcg
     4201 tccacgctac aaggtaggga cttcctatcc agttatctgg gtggacgtac cgctcatgga
     4261 tatctcgtcc agcatggtgc gggacttcat tgcccaaggt cggaaaccca actttctcct
     4321 acctcagcca gtgctagact acatcgagaa ggaggggctc tactgatgtc ctatcaagac
     4381 tatatcaact gctcccgtga ggccttgttg gaaagaatgg cagagcttct acctgagaaa
     4441 cgtctaaccc attgtctagg tgtggagcgt gccgctatgg aattagctca gcgctttggt
     4501 gtagatgttg agaaagcaag tctagcaggt cttcttcatg actatgctaa aaagttgtca
     4561 gatcaggaat ttctagactt gattgaccgt taccagctag atttcgacct caaaaactgg
     4621 ggtaataatg tctggcatgg tatggtcgga atttacaaaa ttcaggaaga tttggatttg
     4681 catgattcag aaatcctgcg agccattgaa gtccatacag tcggtgctgg tcaaatgaca
     4741 gacctagata aggtcatcta cgtcgcagac tatatcgagc acaatcgagc ctttcctgga
     4801 gtagatgtgg cgcgtgagat tgcaagtcta tcgctcaata aggcggtggc ctacgaaaca
     4861 gctcgtaccg tggagtatct agctcatcag ggattcccca tctatcccca aacccttgaa
     4921 acctataacg cctttgtgca ctatttgaaa gagaactaaa tgaagacggc ttttataatc
     4981 attgatgttc agaatgtttt agtagaaact gggttccaga caaaaagtct attgggaaaa
     5041 atttcttatt tacaaaacca ggctagaagc aagaatattg aaattatcta tgttcaacat
     5101 attgagaact ctgaagctca aacatcagaa gattggcagt tatctgcgct tttaaatcga
     5161 aaacctgctg aaaaggtctt tcagaagaag tataacagta ttttcaaaga aactggctta
     5221 aaagaatact tggataagca ggggattgaa aaattagttt tatgtggtat gcagacagaa
     5281 tattgtgtgg atacctctgt caaggttgcc tttgaatatg gctatcagct tattgttcca
     5341 gaaggtgctg tcacaacctt tgatggggat gacattccag cagaaacgat taatgaattt
     5401 tatgaggaca tttgggagga gcgctttgca gatgtcctag attacaaaca tattttctaa
     5461 aaagaggatt aaatgaacga aaaagaatta ctagaactag tcgtgaaagc ggctgatgag
     5521 aaacgtgcgg aggatatcct cgcacttgat gtacaagatt tgactagtgt gacggactac
     5581 tttgtcatca ctagctcaat gaatagccgt cagttggacg ctatcgcagc taatatccgt
     5641 gaaaaagtag ctcaagcggg ctttaaaggt agccatgtcg aaggcgatgc agctggaggc
     5701 tgggtcttac tggacctcgg tgctgttgtc gtgcatatct tttcagaaga aatgcgtgcc
     5761 cattataatc tagagaagct atggcatgag gcgaattcag tagatatttc agaagctctt
     5821 gcttagaaga tgaactcagt tgtagtcaac tgggtttctt tgcttatttt agaaaaattg
     5881 atagaaaggt aaagggctag tggtgtttac catggagctc ttagtatcat gattatggcg
     5941 acttatgaaa cctttgcggc ggtctatgat gcggtcatgg acgatagttt atacgacaaa
     6001 tggacaaatt tttctctgcg tcatttgcct aagaccaagg agaaaaagaa actcttggaa
     6061 ctggcttgtg ggacaggaat tcaatcagtg cgcttctctc aggctggttt tgatgtgact
     6121 ggacttgact tgagtgcgga tatgttgaag attgcggaga agagagcaac ttcagccaag
     6181 caaaagattg cttttataga aggtaatatg ctaaatttgt ccaaggcagg gaaatacgat
     6241 tttgtcacgt gttattcgga ctctatctgc tatatgcagg atgaggtgga agtaggggac
     6301 gtctttaagg acgtgtacaa tgcgctgaat gaagagggag ttttcatctt tgacgtgcac
     6361 tcgacctacc agacagatga agtattccct ggctattcct accatgaaaa tgcggaagat
     6421 ttttccatgc tttgggatac atatgaggac gaagttcctc actccatcgt gcatgagctg
     6481 actttcttta tcaaggaggc tgacggttcc tttagtcgcc acgatgaagt acatgaggag
     6541 aggacctatg agatcttgac ctatgatatc ttgctggaac aggctggatt caagtccttc
     6601 aaactctatg cagactttga ggataaggag ccaacagaaa ctagcacccg ttggtttttt
     6661 gtggcgcaga agtaggagat tagcatgacc atcacaggta ttatcgcgga gttcaatcct
     6721 tttcataatg gccataaata cctgctggat caggcagagg gactgaaaat tgttgccatg
     6781 tcagggaatt tcatgcagcg tggagagcct gctattgtgg ataagtggac aagggcccag
     6841 atggcgctgg aaaatggcgc agacttggta gtggaattgc cttttttagt cagtgttcag
     6901 gcagctgatt tctttggtca aggagctgtg gatatcttgg atcgcttggg cattgatagt
     6961 ctagtttttg ggacagagga agttcgagat tatcagaaaa tcgctgactt atacacagag
     7021 aaaggtgctg agatggagaa atttgtggaa aatctgcctg attccctttc ctatccccag
     7081 aaaacccaag ctatgtggaa ggaatttgct ggtcttgatt tttcaggtaa tacccccaat
     7141 catgttcttg ctctggccta tgcaaaggcg gttgcaggac gaaatatcaa gctgcatccg
     7201 attcagcgtc agggggcagg ttaccattct gtgaataaag atgtggactt tgcctcggcg
     7261 acagccctcc gtcaacatca gaaggaccaa gatttcttag aacgttttat gccttctgtt
     7321 gccctatttg agcaggccag taaggtgatc tgggaagact attttccctt gctccgctat
     7381 caaatcttgt caaatcctga cctaaccacc atctatcagg tcaatcaaga aatggctgtg
     7441 cgcattaaag aagctattaa aacagcccag tctgtggaag aattggtcga gttagtgact
     7501 accaaacgtt acaccaaggc gcgtgtcaga cgcctattga cctatatttt gatgcaggct
     7561 agagaaagtg acttgccaga agccattcat gtccttggat ttactgaaaa aggtaggcaa
     7621 catctcaagt ctttgaaagg tcaggtcagt ctagtcagcc gaattggcaa agaaccttgg
     7681 gatgctatga ctcaaaaggt tgaccagatt taccaactag gaaagccaag tatagcagag
     7741 caaaattttg gcagagtgcc gattagaata gaaacaaact aagtctaccg aaaggtggac
     7801 tttttgagat tttttacgaa taatactctt cgaaaatcaa attcaaatc
//
LOCUS       DAVLTM010000088         7719 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLTM010000088 DAVLTM010000000
VERSION     DAVLTM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.089"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(51..401)
                     /locus_tag="VYN16_001616"
     CDS             complement(51..401)
                     /locus_tag="VYN16_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404123.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(541..876)
                     /locus_tag="VYN16_001617"
     CDS             complement(541..876)
                     /locus_tag="VYN16_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404124.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWIYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(967..2277)
                     /gene="der"
                     /locus_tag="VYN16_001618"
     CDS             complement(967..2277)
                     /gene="der"
                     /locus_tag="VYN16_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1404125.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPGMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2291..3004)
                     /locus_tag="VYN16_001619"
     CDS             complement(2291..3004)
                     /locus_tag="VYN16_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1404126.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3001..3897)
                     /gene="dnaI"
                     /locus_tag="VYN16_001620"
     CDS             complement(3001..3897)
                     /gene="dnaI"
                     /locus_tag="VYN16_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1404127.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3898..5067)
                     /locus_tag="VYN16_001621"
     CDS             complement(3898..5067)
                     /locus_tag="VYN16_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1404128.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSPKETTIIKEAKSKTALQFLAEIKQTRKGTTTQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5068..5541)
                     /gene="nrdR"
                     /locus_tag="VYN16_001622"
     CDS             complement(5068..5541)
                     /gene="nrdR"
                     /locus_tag="VYN16_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1404129.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5681..6046
                     /locus_tag="VYN16_001623"
     CDS             5681..6046
                     /locus_tag="VYN16_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1404130.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6051..6746
                     /locus_tag="VYN16_001624"
     CDS             6051..6746
                     /locus_tag="VYN16_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404131.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6763..7530
                     /locus_tag="VYN16_001625"
     CDS             6763..7530
                     /locus_tag="VYN16_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404132.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
BASE COUNT         2232 a         1708 c         1432 g         2347 t
ORIGIN      
        1 aaatgactgt atctatagct ccataaacaa atcatagata cagtcatttt ttagttccta
       61 caccttcctt tatgcacacc tttagacttc cctagttccc gtctcaagtc aagcattttt
      121 ttgaaacaga tatagtctaa ccagtcacta gcaaaagcga taagtagtaa taatagccaa
      181 ataccccata acttgatatc tgtcaaattt ctcaagacga tattgaaaaa cagaactgaa
      241 aaaactgtcc aagcaaggct aaaaagagca tagagggtga tgtaaatcca gtaaaaatag
      301 taaaaaattg ggaaaaattt actttctcta ttatccttgt caatccagct gaaaaagtaa
      361 aaacagggta ataagattat cattttaaac aagtgttcca tcacaagcct ccttttttaa
      421 attgttttct ttagtttcgt tctattaaat gttattacca atataaacct tggaatagcg
      481 tgtaataaat gattttagag gtaccataag ccacctccta caaatagaaa ccgatataaa
      541 tcaatgcctt ccacccttag acttccctag ttcctgtctc aagcgaaaca tttctttgaa
      601 acaggaataa gttaaccaat tcataccaat agctagcaga ataaaaagaa accaaatgcc
      661 ccataacttg atatctgtca catttctcaa gacggtattg aaaaacagaa ctgaaacaac
      721 tgtccaagca aggctaaaaa gagaatagaa ggggatgtaa atccagtaaa aataataaaa
      781 aattggaaaa aacttactat ttctgttggc cttttcaatc cagttatcaa aataaaagta
      841 cggtgctaaa agtaagaatt taaacaaatg ttccatcacc gacatccccc tccttctttt
      901 gatagcgttt tctattattt tattatatca aaaaaatccg gaactgtcat tccagattct
      961 acttttttat ttgcgttttc ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc
     1021 cttgcggatt tgattttcca agaaacgtag gtaagaaaag tgcatgagtt cttcttcgtt
     1081 gacaaagata acaaaggttg gtggtttggt tgccacttgg gtcgcataga aaatcttgag
     1141 acgttttcct ttgtctgtcg gtgttgggtt gatggcaatg gcatccatga tgacatcgtt
     1201 caagacagct gatggaatac gtgtattttg actttcgctg atttgcttaa tcatcccagg
     1261 aagtttgtgg agacgttgct tggttaaagc tgatacaaag ataatcggtg cgtaaggcag
     1321 gtattggaac tgctcacgga tatcttcttc ccagtttttc atagtgtggt tatctttttc
     1381 aagcgtatcc cacttgttga ccacgataat catcccttta ccagcttcat gagcaaatcc
     1441 agcgatacgc ttgtcgtatt cacgaatccc ctcttccgca ttgatgacca ttaagaccac
     1501 atctgaacgg tcaatagcac gcatggcacg catgacagag tacttctcag tattttcata
     1561 aaccttacca gacttacgca taccagccgt atcaatcatg gtaaactctt gaccatctgt
     1621 atctgtaaag tgggtatcaa tagcgtcacg agttgttcca gcaacaggac tggcaataac
     1681 acggtcttcg cccaagatag cattgatcaa gcttgatttt ccaacgttag gacgaccaat
     1741 caggctaaac ttaatgacat ctggattttc ttcctcatat tcatttggaa gattttctac
     1801 gatagcatct agtacatccc ctgtaccgat tccatggaca gatgagatag gcagtggttc
     1861 acccaaaccg agagcataga aatcatagat atcatttctc atctcagggt tgtccacctt
     1921 gttgactgcg aggataactg gtttgtgggt cttataaagc ttacgggcta cgtattcgtc
     1981 tgcatcggta attccttcct taccggacac gacaaagacg ataacatctg cttcttccat
     2041 ggcaatttct gcctggtgct tgatttgttc catgaaagga gcatcgacat catcaattcc
     2101 tcctgtatca atcatgctaa aagaacgatt gagccactca cccgttgcat aaatacggtc
     2161 acgtgtcact ccttcgacat cttctacaat ggagattcgc tcaccagcga tccgattaaa
     2221 tagggttgat ttcccaacat tgggacgtcc tacaatggca atagttggta gggccataat
     2281 ttctcacttt ctacaataat ttcttctgtt caagattttt tctagttgag cttggttcag
     2341 cttgaccaaa ctgttctgct aggcgctgac tccagcttgt ggtcgcacgc gccccagcat
     2401 agtcagcctg aacacggtca taagcttgga ttgcctcagt tgactgttct tggtattctt
     2461 cctcaaagac aacattctct agtggcagtc tcggtttcat atcatgatgt tgatttggca
     2521 cacccagtgc catcccaaag acagaatagg tgtagtcagg caggttaaag agctctgcca
     2581 cttcttcaga cttgtatcga accaaaccga taatcacacc accatagccc aagctttcag
     2641 ctgccaacaa ggcgttttgt ccagcaagag ctgcatcgac cgaactaatc aagagacctt
     2701 ccacaccttg gggttggaag gtgtcggtat gaagtcgggc tcccttttct gctcggttca
     2761 aatctccgac aaagagaagg aaaacagcag actggcgaat ggcttcttga ggtaccaatt
     2821 catacaaggc atctttcttc tcttgacttc gtaccacaat cacagagtag gattggaaat
     2881 tcttccaaga tgatgccatc tgggctgctg tcaaaatctc atttaagtct acttggggaa
     2941 tttcttgctc tttaaacctg cgcactgaag tatgagcctt catcaattta atggtttctg
     3001 tcatcgacgg tttactcctt ctaaacgagt ctcctcagcc aaataacgga tgcgttccat
     3061 gacccgtctg gcttcccagg tttcgtcatt tccatttttc cctttagcga aatgcttctc
     3121 caaatcttca aagttgaagt tagaggtgaa aaaggtcggt aaattttcct gcatccgata
     3181 ttggagaatg acctgtagga tttcatcacg cacccaagcg gtggattgct cggcaccaat
     3241 atcatctaaa atcaggactt cagaaagctt aatctcatcc actaaggtct taacattgcc
     3301 atcaccaata gcatttttga catcaatgac aaagctagga tagtggagga gagtggatga
     3361 tacaccacgt ttttctgata aatcatgagc tagggcagcc accatgaaac ttttacccac
     3421 accaaagtct ccatacaagt aaagaccttt tcgaatagct ggatattgct ccacgaaggc
     3481 tagtagcttt tcaaaaactg gtaagcgccc caaatcatcc aagtcaactt gagccaaact
     3541 agctttcttg agactggctg gtagattgat taacttgaga cggttcttaa tagccgcttc
     3601 tttttcagcc gcgattagct caggagtttc ttcatatgaa acatctgcat agccatgatt
     3661 cttaaccaaa atcggcttgt agcctttggc aatataatcc gtatccccac ggagaaactt
     3721 gtcacgctcg gtgatgtact gattaaactt ggagatactg cgatttaatt cctctggagt
     3781 caaggattct tgctggataa aagccgcaac atcagggtcc ttcatgattt tctggaccaa
     3841 atcttgataa taaaaacggc tgggttgacg tttgagtacg tctccgacac tttccatcta
     3901 atctcctcct ttttctaatc gagctaatag ttcttgcttc ttacgttcta gttccagacg
     3961 agtttcctcg ctggtttcat tcttatattc aggattactc catttaggaa cattggtttt
     4021 tactggggca gtctgattct gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg
     4081 caagactgcc tcttctgccg aatgaatctt ttgataggca tagtcattgg ctaccttcat
     4141 ggcatatttc tcattgatat ttgccgaatc gaccttatta aaggtcaata aaagaatgat
     4201 attgatgact tcgtccagca agcccaagcc agccatctgt tgcaagagtt ctctttctgt
     4261 ttgggtagtg gttcccttgc gtgtttgctt gatttctgcc aagaactgca aggcagtttt
     4321 acttttagct tccttgataa tggttgtttc cttaggacta aagtccgagg aaactggttt
     4381 ttgagcaatt ttttcccgca tgcgtttggt tgaaataacc tgggaaacag ctgttgactt
     4441 ggccaattga taggtctcaa accaagtcca cttcttctcc tcagcaatag caaacaagtt
     4501 caagacatca gactgctcat ccgcaaaacg aagtccatct cgagccatca gctgacgaaa
     4561 atgatccaag tcaaaatcat tggccacttt cttcttgaga ccaaggtctt cttgactgcc
     4621 tagttctgcc aattctggaa agacttgatt gagtgagaca ggtatttctt caccatcagc
     4681 actttcaact ttcaaatcct ccacagcaga gtcgccaatc tttttctcta agagtctgcg
     4741 ataaacagga tgctccaaga agtcttgact agatagagga gcatggaggg ctagctgata
     4801 aacatccccc ttttgataga gggtcaagag attaaaagca gataagattt tcaatgattt
     4861 tatcagtcta tccatcccaa agttgagatg attgaggata cttgaaaaaa gatattcctt
     4921 tctaccatta tcccaaaaac tgattgtata aagataaagg ctcagtgcct cctgaccgat
     4981 aatcgggagg tagcactgta ccagagatga ggtatcttgc gacacccgat tattctttag
     5041 ataagaaaaa cggtcaattg gcttcattta tctttccttt ttctttttag aggactgggt
     5101 gatttgttgg agcaagctct ctaactcact gacatcctta aaactacgat agacactagc
     5161 aaaacgtaca taggtaatct cgtccaattc agccaactcc tccatgacga gtgaaccaat
     5221 gtcctcactt tgaatttcat tttcatttcg accacggagt ttctgttcga tacgattgac
     5281 taccatgttg atttcatcac ttgacacagg acgtttctgg gctgagcgga taatcccatt
     5341 aaagatttta tctctggaga attgttcccg tgtgccatct tttttaacaa ccactaaggt
     5401 tctttcttct actcgttcgt aggttgtaaa acggtgttgg cactcgtcgc actctcgtct
     5461 tctacgaatg gtgttccctt cttctgcttg gcgactatcg ataacacttg acttggtagc
     5521 cccacatttt ggacaacgca tcctttccct ccttatcgtt ttcttttcat tataccattt
     5581 tttaagcgat tcccaaaaca attcttcttt ttgcttgaca agttttttgt tttgttgtat
     5641 tatttaatta agacaaaaga ataagagaaa ggagactaag atgtcctgga catttgacaa
     5701 caaaaaaccc atctatttac agattatgga gaaaatcaag cttcagattg tttcccatac
     5761 actggaaccc aatcaacaac ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa
     5821 tcccaatacc atccaaagag ccttatcaga ccttgaacga gaaggatttg tctacagcaa
     5881 gcgaacaact ggacgatttg tgactaagga taaggagcta atcgctcagt cacgcaaaca
     5941 attatcagaa gaagaattgg aacacttcgt ttcctccatg acccattttg gctatgaaaa
     6001 agaagaacta ccaggcgtag tcagtgatta tattaaagga gtttaagcct atgtcattac
     6061 tagcatttga aaatgtatcc aaatcatatg gagcaacacc agcccttgaa aatgtttctc
     6121 ttgacattcc agctggaaaa attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa
     6181 ccctgattaa actaattaat ggcctcttac aaccagatca aggacgtgtc ctcatcaacg
     6241 acatggaccc aagcccagca accaaggccg ttgtagctta tttgcctgat acgacctatc
     6301 tcaatgagca aatgaaggtc aaagaagccc taacctactt caagaccttc tataaagatt
     6361 tcaatcttga acgcgctcat catctacttg cagacctggg cattgatgaa aatagtcgtc
     6421 tcaagaaact atcaaaagga aacaaagaaa aggttcaact gattttggtt atgagccgtg
     6481 atgctcgtct ctatgttttg gacgaaccca ttggtggggt ggatccagca gcccgtgctt
     6541 atatcctcaa taccattatc aacaactact caccaacttc taccgttttg atttctaccc
     6601 acttgatttc tgatatcgag ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag
     6661 tcgtccgtca aggaaatgta gatgatattc gctacgagtc aggtgaatcc attgaccaac
     6721 tcttccgtca ggaatttaag gcctaagcaa aggagattat ttatgttttg gaatttagtt
     6781 cgctacgaat ttaaaaatgt taacaagtgg tatttagccc tctacgcagc cgtgctagtc
     6841 ctttctgccc tcatcggaat acagacacaa ggctttaaaa atctacctta ccaagaaagt
     6901 caggctacta tgctactttt tctagccaca gtctttggtg gcttgatgct tacacttgcg
     6961 atttcaacca ttttcttgat tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc
     7021 tatctgactt tgaccttgcc agtttctgaa caccatatca tcacagccaa actaatcggt
     7081 gcctttatct ggtcattgat tagcaccgct gtattggctc taagtgctgt tattattctg
     7141 gctttaacag ctccagaatg gattcctctt tcttatgtga ttacatttgt agaaacacat
     7201 ctccctcaga tctttcttac aggtatatcc ttcctactaa ataccatttc aggaatcctc
     7261 tgcatctacc tggctatttc cattggacag cttttcaatg aataccgtac agcactcgct
     7321 gttgcagcct acattggtat ccaaatcgtc attggattta ttgaactttt cttcaatctt
     7381 agttctaatt tctatgtcaa ttcactggta ggactcaatg accatttcta tatgggagca
     7441 ggtatagcca ttgttaaaga actcatattc atagctatct tttatctcgg aacctactac
     7501 atcttgagaa ataaggttaa tttgctttaa ataattttta cctagatatg taacatactc
     7561 atagaacaaa agaaaccagg caaaaagtct ttaaaattag aaaacgcata gtatcaagtg
     7621 ttaaaaatcc ttgatattat gcgttttttg tgagctgact tatttccttt cactatatcg
     7681 caaaatgaaa taagaacgga acgatgagat tttggaatt
//
LOCUS       DAVLTM010000089         7648 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLTM010000089 DAVLTM010000000
VERSION     DAVLTM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.090"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            8..136
                     /gene="blpC"
                     /locus_tag="VYN16_001626"
     CDS             8..136
                     /gene="blpC"
                     /locus_tag="VYN16_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1404133.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(207..1547)
                     /locus_tag="VYN16_001627"
     CDS             complement(207..1547)
                     /locus_tag="VYN16_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1404134.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLAFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(1561..2298)
                     /locus_tag="VYN16_001628"
     CDS             complement(1561..2298)
                     /locus_tag="VYN16_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404135.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2303..2641)
                     /locus_tag="VYN16_001629"
     CDS             complement(2303..2641)
                     /locus_tag="VYN16_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404136.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2918..3247
                     /locus_tag="VYN16_001630"
     CDS             2918..3247
                     /locus_tag="VYN16_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1404137.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3415..4464)
                     /locus_tag="VYN16_001631"
     CDS             complement(3415..4464)
                     /locus_tag="VYN16_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404138.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4461..5192)
                     /locus_tag="VYN16_001632"
     CDS             complement(4461..5192)
                     /locus_tag="VYN16_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404139.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLTPYSGSININGLTLQEDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVTFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5260..5670
                     /locus_tag="VYN16_001633"
     CDS             5260..5670
                     /locus_tag="VYN16_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1404140.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5680..5967
                     /locus_tag="VYN16_001634"
     CDS             5680..5967
                     /locus_tag="VYN16_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404141.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(6334..7470)
                     /gene="dnaJ"
                     /locus_tag="VYN16_001635"
     CDS             complement(6334..7470)
                     /gene="dnaJ"
                     /locus_tag="VYN16_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1404142.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         2441 a         1717 c         1421 g         2069 t
ORIGIN      
        1 tcataacatg gataagaaac aaaacctaac ttcatttcaa gaactaacaa ctaccgaact
       61 caaccaaatt acaggtggag gattgtggga agatttatta tataacatta atagatatgc
      121 tcattacatc acataagaac ttcatcatcc aatacaacta taaaaaaata agaccgagaa
      181 acaagtactc tcggtcttat ttttcatcat tctgtatgta tcacagtaag tacctgacga
      241 aagacttgat tttgacaggt ggtatttaga ctggtattag gatggctttc cacaatcttc
      301 atgacggtat agagaccaac tcctctctcc tcccctttag aactggctcc aaaggagaag
      361 atttcagaaa tatcgatgcc ctcttctttg atggagtttt cgatgataaa ggtctcctgt
      421 gctccatttt ttaaaaaggc gattgaaaca tgaggttgac tagcttccac actggcttca
      481 atagcattgt cacaaaggat agacacaatg gttagaaaat caagtagact catcccctcg
      541 acctgaatct cctcaggaac ttcgacatta aagacaatgt tcttatctct ggcttttaaa
      601 aatttccctg ctagaagact tttgagggct ttatcacgaa tatttaccaa tctgcccagg
      661 tcatatttat tgttctgcaa tttctgactg gaatccttta agacggagcc atagacctct
      721 tttatctgct ccatatcctc ctcttcaatg cccagacgta agctagtcaa gaggttggta
      781 taatcatggc gaaaactccg tacttccttg taaagctcct ctatatgccg actatagcgt
      841 tccatatctc tatagcgcag ggcctgctct tgttccaatc tctcatagag tttttccttc
      901 aaataggtat ccaatttctt gataaccccc ataaaaaaga gtaggtaaaa gactaggatg
      961 agatggcgaa cagtctttga ttgaatactt tgttcatatt caaaaaaaga cagactttcc
     1021 atgactagat agtagccacc cattatccag ttaatctgag tcagggactt ttgaaaggct
     1081 ttatcgagaa tctcctttct caagctagta aaatcgtagt ccaaccattt caaaaaagct
     1141 agagaaatga agaaattgaa aattattata cataacccag taaatgagta gccatcatat
     1201 acttgccctt gtcccaaaaa tggaagcaca aaataggaga ctcctctata aaagagattc
     1261 accaatatca ttggaaagag accataaaag aaaaggagtt ttttaggaag ccctctcaat
     1321 aataagaaag ataagcctat gccgtacaag ggttccataa aataagatag gtaaacattt
     1381 cctactatat agctaatcat cacaaaaaca aaggccaaca gtatcttaaa aagaaaggcc
     1441 ttaaaaatcc tctcgaaagc aagatcaatt ccatccacct taaagaagat gacaatttct
     1501 agtccattag taacaagtgt atacaacaat atccaagcaa tgttcataaa ttctcctagc
     1561 tcagtgtaat ttattgatgg cctcagacac ttccctgacc ttataacggg cgattagaca
     1621 acttccacca ttgggaaaga aaagcagttt ttctttctta tccaaatgca ccacatttgc
     1681 aggattgatg agaaaagagc ggtggcactg caagagacgg ggctcctgct tgaaaacctc
     1741 ctctaaactc gctgtaaatt ccagcctgtc tgtcttggta tagagaataa cacgatgggc
     1801 tctgggcgac gtttcgagat agtaaacctc tttaaaagga tactgaaatt gggcaaattt
     1861 tgatttaaag taaaagcaat cttccgccag acttttacta tcttgactat tggcatagag
     1921 gagggctgtc tcgatccgag attcaaactc ctctgctgac aaggccttat caatgtagtc
     1981 caaagcagac acttggtagc gaaaagacag gggcataaac tccgagtgag tcgtcacaaa
     2041 gacaatcagg gcataaggat cccgatcccg aatctttcta gccacttcca gtcccttcat
     2101 ctcttcattt cgaatctcaa tatccaaaaa gaatagctga tgggccccct tctcatgcac
     2161 ttcagccagc agttggtccg gcttgccaaa tacctcaaaa gagctaggaa tgatatgatg
     2221 tgctttcaaa agtttctcaa tcgtcgtttc aattctagtc tgttgggaaa aatcatcctc
     2281 taaaacaaat attctcatct tcttactctc cctctttcaa ccatttttga cgaatttctc
     2341 tatagcgacg tctggcatac ttgacagcgt attgaccttc ttctccgaga aaaaggattc
     2401 tttcttgaaa atcaatcgat tttaatttat caagattaac caaacaattt cgatgacatc
     2461 tcatcaaggt ttccccacat tggttctcaa gattacttaa attttgaagc atattaaaac
     2521 tagcttcttc tgtaacaatc tgtacggtat gggctttagt tggatgtgtt tggatatagt
     2581 agatatcgtc gatgtttact ttatagactg ttttctgcgt ctgaataatc atatatttca
     2641 ttggattctc ctcctaagaa aagaataaca gaaaaggtct agcccaagca cacccaatcc
     2701 taaacagtct ttgaaaactc ctaaaaggat tatttttcta caacatcatg atgagcttac
     2761 aactatttta tcataaaatt cactcagcgc aattcaagac atttcaatga caattaaaga
     2821 tttgaataaa aaaaaagaat taaaaatgat aaactaacag tatcaaaaca cagttgccag
     2881 aagtaagact ggcacccaga ttaaaggagg cattcccatg acagatacag accccatcaa
     2941 aagagctcat actttgatta ctgacttaaa caaagcctac caagcatgca aacaggcaag
     3001 cgctgacgac gtccgctttc aggagcaatt aaactctatt cttggttttc tagccaaggc
     3061 tgaaacagtg gataatcgat tcttgattga actagaaaaa ttttaccaga cttccagtct
     3121 tctcatggga ctcagcgctc ttgatccaga tgctccaact cgcgccgctt ggcgggccta
     3181 tgaccgcttc cactttgacc aagtcaagac caagttaata ctcaatgaaa atcaaagagc
     3241 aaactaggaa gctagccgca agctgtactt gagtacggta aggtgacgct gacgtggttt
     3301 gaagagattt tcgaagagta taagtctcta cggaccaacc attatcctgt agtaacaaaa
     3361 aagaagctga gacagactga actcaacttc ttttttagtg tcgtatcagc aatgttagtc
     3421 ctgcatctga cgttttacct gataaggcaa atacaagact agtaaaacca aaccagctcc
     3481 tagtaaggct agaagggcca gtttttcttg gaaggtaatc aacccaacaa ctaattccac
     3541 aagtaaaaca aaactggtca atcctcgaac tacctcctgt aagccttttt ccttttgccc
     3601 cttgtccagc ggaaagagtt gggtcaaata ctggtagtca aaggcatgat agagggccag
     3661 caactggaag agcaagaggt agttaaagag aactaccact gctgtcgcaa tccaagcttg
     3721 ctcgataaaa acctgcgcca gtacggaaag taatagaaga cgaagactga gagcaaagag
     3781 gtcgccattt cgcagataag aacgcagata gagattttgc caaatcttcc caggcacctt
     3841 ctgaacagcc tttaagataa agtccagata ggcacgacgc ttaacgctgt ttgaaattcc
     3901 cttgacctgc gtaaagaggg caaagaaacg aagcaagact tgcttacgct tgctttcttg
     3961 agaaataaca tagtcccagt ccagtccagt ttcagtgaaa aatttgctgg ccttttgacg
     4021 aaagtggaaa tattttccta cccccaataa aagcacatag agcagaaaaa ctggcaagcc
     4081 ataacccatt gctaaaaata aaggcgcaaa taacagcaag aaaagggtct gtacaaagag
     4141 ccaaaagact agggaaatgc cagtttgacg cttgagatgg agcttgattt cctcttctcc
     4201 aactaagaga aagagcttgt ctggagcctc catataggtg gcaattcctc cccaaagtaa
     4261 aagtaaaaca gacgtaattc ctacaaacaa aaggataggc caatgatttt caggaaaatg
     4321 ttgtaagagt tgactgtact ggtaggctag aaagcccaac aggacaagca ggaacaagac
     4381 aaagtggtca ttgagcacat agcgcagata accaagacac tccttacgaa aggcctgctt
     4441 tctctttaaa aacaagtctt tcatagatcc tcctctttgg tcagagccaa gtaaatatca
     4501 ttcaaactag cctcaggcat atcaaaggct tcacgtagtt gcaggagatt gcctttggca
     4561 cgcacctctc ccttgtgaag aatgacaaag gcatcacaca tcttctccgc cgaatccagc
     4621 acgtgggtac tcatgagaat agacttgccc ttttgcttct ccacttccaa aagctgaatc
     4681 aaatcagaaa tagccagcgg atcaagacca aggaaaggct catccacgat gaaaagactt
     4741 ggatccacca caaaagcaca gataatcatg accttctgct tcatcccttt tgaaaaatga
     4801 acagggaacc agtctaattt ctgttccaaa cggaacattt ttaacaaggg ctctactcgt
     4861 tcgaaagtca ctttttgctc aataccgtaa gccatagcaa ccgtttcgat atgctctctg
     4921 agagtcaatt cctcatacag actaggcgtc tcaggaatgt agccaatttg cttgcggtag
     4981 ctcgttgcat cttcttgcag ggtcaagcca ttaatattga tggaaccact ataaggtgtc
     5041 aacagaccga taatctcatt aatggtcgtc gatttaccag caccattgag accaatcaaa
     5101 ccaaccagct gcccactttc aacggtaaag gacacatctt tcaaaacagg aacatgaaca
     5161 tagccacctg tcaggttttt aatttctaac atattttctc caaatctggt ataatgtagc
     5221 tatattatat caaaattcag tacagtagag gtagatttta tgtcagattg cattttttgt
     5281 aaaatcatcg caggggaaat tcctgcttca aaagtatatg aagatgagca ggtccttgcc
     5341 tttcttgata tctctcaagt aacactagga cacaccttgg tcgtgccaaa agaacactat
     5401 cgcaatcttt tggagatgga tgctacgagc gccagccaac tctttgccca agtaccaaaa
     5461 gtagctcaaa aagtcatgaa agtcactaag gctgctggta tgaatatcat ttccaactgt
     5521 gaagaagtcg ctggtcaaac agtttttcat actcacgttc accttgtgcc tcgctacagt
     5581 gctgacgatg acctcaagat tgattttatc gcccacgaac cagactttga caaacttgct
     5641 caagtcgctg aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc caacctactg
     5701 ctatttgcag gagctgcagc cggaagttat ctggttacaa aaaatcgcca aaccatcaca
     5761 gatgaagtct tgaataccac tgaccgcgtt caagctatca aagacgatgt ggatattatc
     5821 caaaatagcc tgcaaatcat cgaccagcaa aaagaactta tcaaggaata ccaagaagac
     5881 ttgacttaca agtttaaggt cttggaaaag gatatccaga ctagactagc tgtgataaaa
     5941 gaaatgcagg gaactgaaga taagtaaaaa gagccccccg gctctttttt aaaacgtaaa
     6001 caagttatag tgaattgaat ttagaatagc acatcatagt ttctaaagca ttgttagaga
     6061 ttactttaaa atcctcttat caatttgttc atattctatt tcaatctact atatatatcc
     6121 catcaactat gcatcataat ttaggtaact catactcaat aaaaatcaaa aagcaaacta
     6181 gaaagctagg cacagactgc tcaaaacacc gttttaaggt tgtggataga actgacgaag
     6241 tcagctcaaa acactgtttt gaggttgtag atacgactca catatatacg gcaaggcaac
     6301 gctgacgtgg tttgaagaga ttttcgaaga gtattattct ccatcaaagg catcttttat
     6361 atggtcaaag aagccttttt tctttggatt tactttcaag tcaccagcag ccgcgaattc
     6421 tttcaaggct actttttggc ggtcgttcaa gcctgtcggt gttacgacat taacagtaac
     6481 gtattggtca ccaactgcac cgccacgaag gctcggtgcc cccttactac gtaggcggaa
     6541 tttcttacca gtctgagttc cctctggaat aaccaattca acatcaccgt gaacagttgg
     6601 aatatctact gtatcaccaa gagccgcttg gacaaagttg aggttgagat tgtagaagat
     6661 agtcgttcct tcacgttcaa acttgtcgct agcttctaca gaaactacta catacaagtc
     6721 cccataaggt ccaccgttaa agcctgcttc gccttgacca gcgaggcgaa tttgttgacc
     6781 tgtttccaca ccagcaggga ttttcacatg tacgctatga gcttgtttct catgacctgt
     6841 tccatgacag gttgtacatg gatatttgat ttcttttcct cgaccgtgac agacatcaca
     6901 ggttacttgg cgacgcatca taccaagagg agtctgcgta tcgacgttaa tgacaccagc
     6961 gccatgacag cgtccacaag tgactggact tgtccctggc ttagcaccag agccattaca
     7021 tgtacgacag ccagcttcac gatgatactt aacttccttc tcagttccga agatagcttc
     7081 ttcaaaggtc aaattgacac gatactggag atcatctcct tggcgaggag cgtttggatt
     7141 gcgcgaagaa ccgcctccgc cgaagaaact tgagaaaata tcctcaaaac caccgaagcc
     7201 acctgcccca ttgaaaccac cgaaaccacc agctccacca aaaccaccat tggcgcctgc
     7261 agcaccatac tggtcatagg cagcacgttt ttggtcgtca ctcaaagtct cataggcttc
     7321 ttgaacttcc ttgtacttgt cctcagcacc aggctccttg ttgatatctg ggtgatattt
     7381 tttggaaagc ttacgataag cctttttgat ttcgtctgcc gaagcgtttt tggatacccc
     7441 cagacgatca taaaattcag tattgttcat acaagatacc aagaccgtaa aattcgactg
     7501 aaaaatagga aatctgacgc tggagcgacg ctcctagaca gatttctctt ttttccgaga
     7561 atttaggtcg ggttcaattc ctttctttta tattgatgag ataatactcc agccactcgg
     7621 ccctatccct ttttatcgaa aaacagag
//
LOCUS       DAVLTM010000090         7568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLTM010000090 DAVLTM010000000
VERSION     DAVLTM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.091"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(107..202)
                     /locus_tag="VYN16_001636"
                     /pseudo
     CDS             complement(107..202)
                     /locus_tag="VYN16_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(279..590)
                     /locus_tag="VYN16_001637"
     CDS             complement(279..590)
                     /locus_tag="VYN16_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1404143.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(515..757)
                     /locus_tag="VYN16_001638"
     CDS             complement(515..757)
                     /locus_tag="VYN16_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1404144.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(758..1390)
                     /locus_tag="VYN16_001639"
     CDS             complement(758..1390)
                     /locus_tag="VYN16_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404145.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(1404..2399)
                     /locus_tag="VYN16_001640"
     CDS             complement(1404..2399)
                     /locus_tag="VYN16_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1404146.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKFPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERIEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2396..3094)
                     /locus_tag="VYN16_001641"
     CDS             complement(2396..3094)
                     /locus_tag="VYN16_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1404147.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(3171..4181)
                     /locus_tag="VYN16_001642"
     CDS             complement(3171..4181)
                     /locus_tag="VYN16_001642"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1404148.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SDQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(4165..5172)
                     /locus_tag="VYN16_001643"
     CDS             complement(4165..5172)
                     /locus_tag="VYN16_001643"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1404149.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(5159..6112)
                     /gene="mvaD"
                     /locus_tag="VYN16_001644"
     CDS             complement(5159..6112)
                     /gene="mvaD"
                     /locus_tag="VYN16_001644"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1404150.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(6094..6972)
                     /gene="mvk"
                     /locus_tag="VYN16_001645"
     CDS             complement(6094..6972)
                     /gene="mvk"
                     /locus_tag="VYN16_001645"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1404151.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(7094..7423)
                     /locus_tag="VYN16_001646"
     CDS             complement(7094..7423)
                     /locus_tag="VYN16_001646"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1404152.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFCKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
BASE COUNT         2169 a         1813 c         1414 g         2172 t
ORIGIN      
        1 tcttaatcta ctatagaatc aagaaatcta ccaccttctt taaataccct ccattatcac
       61 ataaacaggt aaacttttca attaatgact gcgcttttca atcacgctag aggtacttac
      121 ttgcttcttt gatactaagt tcagccattc tttccttgtt tttctcaata aagcatgtta
      181 cccaagtggg attcgttctg gtggtcaata gcgacacgtc tcagccagat attatctgat
      241 aggctccatt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcagattg
      301 ctcaaaacac tgttttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag
      361 agtattaaga ttatttcttc tagttcagag tgttcataca ccaaactccc tactactcga
      421 tctaggatat ctaccgtgtc ccaccaggat tttgtcacga ctaactgctc tagcttaggc
      481 aaatcggttt cctttagata agactgcatt gctttcaaat agttagcagc cacatattgg
      541 tattctctag gatccttttc ccagcaagtg tctgcaaaat cccaatcgat aatctttgtt
      601 tttttcgctt ctggaaaata tttttataga gtttatttct ttcaggcacc gcaataccta
      661 gaaaagaaaa ttgatggcgc atataggctt ccatggacct tgctttttta gagtcttttg
      721 ctgcttctag ctcctcaagt aaatctgcta aactcatcta aaactcctct tgccccacca
      781 aatggtgctg aaaggcatag acagccgcct gggtacgatc gctgacttca agtttggcaa
      841 gaatattgga cacgtgggtc ttgaccgtct tgagagagat aaagaggtca tctgcgatgc
      901 gctgattttc gtagcccttg gcgatgagtt ggagaacatc tcgctcacgc gcagtcaatt
      961 cttcatgaag ttccatatga ttgcggtggt attcaacctt cttgctaacc tcttgctcaa
     1021 tggccagctc gccagcagct accttactga cggcatgaag caattcatct gcactagaag
     1081 tcttgagcat atagcctttg gcaccagcat ctaagactgg catgattttt tcattgtcca
     1141 aataagaggt cacaatcaaa atcttggctt caggccattc tttaaggatt gctaaggttg
     1201 cgtcaatccc attcatctca ggcatgacaa tatccatgac aatgacatct ggacgcagtt
     1261 ccaaggccaa gtcaatccct tgagacccgt tggacgcctc acccacaact tctacatcgt
     1321 cttggaggtc aaagtagctt ttcaagccca atcggaccat ttcatggtca tctactagta
     1381 aaattttcat ctttactcct ttatcattcc ttatctaaca ggggaatacg gatatcaacc
     1441 gccagccctt gcttgggagc tgtcaagagt tgaactgttc cagccatatc ttcaatccgc
     1501 tccttgatat ttcgcagtcc ataactcaag tcgtctaagc tccctaactg gaaaccaatc
     1561 ccattgtcca ccaccttcag ttgcaattca acatctgtct gatagaggta gacatctagg
     1621 caagatgcct gggcatggcg gagcgtattg ctaatcaact cttgcaggat acggaagata
     1681 tgctcctcga ttttcttagg aaatttcgtc atattctgct tgagactaac cctaagatca
     1741 ctcttgtcct caagctcttt taagagaatt tgaatccctt ctatcaagct cttctgctcc
     1801 agttcaactg gtcgcaaatg caagagcaaa acccgcaaat ccttctgggc tgtttctaaa
     1861 atagctgtga cactctgcaa ctgggtctgc atcttttctc tatccaattt caaagcctgc
     1921 tgactgatac ccgataaaat catgtgggcc gcaaacaact cctgactgac tgtatcgtgc
     1981 aaatcccgag caattcgctt ccgttccttc tcgatgattt cctcttcctg agcaaggcta
     2041 tgattttcag ctttttgaag agcctctgtc aaaaggttaa gtttacctga taaggacttg
     2101 aaactggcat ccaaatctgg atctgcaacc tgaaccactt cttgccctgc caataaacgc
     2161 ttgagattag cctgcatttt tcttagagaa agctcttcga tccctcgcca aaacagggct
     2221 aagagacagg ttatggacat gctgaaaacc aacaataaaa agacaaattt ttctgttttt
     2281 tcgacatcgt gcaaaaagat agaccagtca aaatcaagta tttccagcaa gctgtgggag
     2341 aaaaaaaaga caaacaggaa ggaggtgaga gcaataatga cataggcttg ttttttcatc
     2401 ctctaaccac ctccacatca ccaatcatag tggtcaagaa aatcttgaca ctcttgttac
     2461 tcttgagata gtcttttgtt tcttgatgat agtgttcatt gcggagggct cgcttgggct
     2521 ggttgaaaaa agtcaaatcc ccatagagac agttaacgct gagactgact tccacatcta
     2581 caggtacgat gattttggtc gttcctacca tctttctgag gataatgaca ttgtcatgat
     2641 tggttaagat gaccctctcc agatgaatag tgtccttgcc catgaagcga aagagattga
     2701 tatcatcgaa ttggcaagtc tggtagcttg aaaaatgatg aagatttcca aaccaacgat
     2761 ttttctcctt cttaaccgtc acgacctctt caaaaaccaa attggtctgc tctttttcct
     2821 ggttcatcat cgggtaaaga agaaagaggc tatagataac cgcaacaaaa atagctagaa
     2881 tcacaaaagg attgagcata acgatgaaaa agaagagaat ggttgccgct actaaaagaa
     2941 gattatttcc ctctttacca gtgtagtagc gaatcaaaag caaaaagagg aatagtatca
     3001 gcagaaaacg cgaaaaatgc tctgatacca tcaaaatcag agctcctgtc agaagacagg
     3061 cttcgataaa taaaaagatt ttaaattttc tcataggttc atcctctccc ttctatttta
     3121 tcacaattca aaaaagtcac ctcagtctga ggatggaaaa aaggcgctgg ttacgccttt
     3181 ttcatctgat cctttgcttc ttttaatttt ccataaagaa gatagtctac tttttgtaga
     3241 tctgctatgg tggcacagtt aagggaacac ataatcaagc gtaggtctgc tttccagcct
     3301 tggacaatgc caatcacttc ttcaactgtg taggtttcaa ccaattccag aacggttcgt
     3361 gacaatccca cagacttagc accaaaaacc aagcacttaa tcatatccag cggattccga
     3421 acccctccac taaccaagag ttcgacctta tctttccatt cttgggcatt gagaagggcc
     3481 tgcatggtag actgacccca ttgattgagg taatcacgct ggtcactacg acggttttcg
     3541 atataggcaa agctggtgcc accacgaccc gataggtcca ctgtacgaac accgaattca
     3601 taggctcttt cgattgtctt ggcatccatt ccaaagccca cttccttgag gacaatagga
     3661 acgggaattt gctcgctata atctgctaga tgcgattgcc agcttctaaa cttcctttct
     3721 ccctcgggca tgagtaattc ctgcatgaca ttgacatgca cttgcaatag aacaggattc
     3781 atctcttcta cagtctgaag tcctaactcg acaggcttgt ccaatccaat attggttcca
     3841 aggaggagat tgggatgact agacttgaca gaaaaagaat catccgttgg atttttgagg
     3901 gctgcgctat aagaacccgt tacaaataaa ataccacagg attccgccac ctgagccagc
     3961 ttttgattga tttctcttcc cttattactt ccaccagtca tggcattgat ataaaaagga
     4021 aagtcccact ttcgaccagc aaactctgtc gaaagatcga tttcatccag attgtaaaga
     4081 ggcaaggaag aatgaatcag ctccacctca tcaaagctat tataggaact tttctgctca
     4141 agggcataga ggatatgctc gtccttacga tttgtcgtca tgtcctatcc tttcttgata
     4201 taagagctca atccccagat cggcccaacg attttttaag gttttggttg attgcgcatc
     4261 aaaactcaag gcgatgccac agtcaccacc accagcacca ctactcttgg caacggcctg
     4321 caaatcttga ctggcttctt tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa
     4381 gccttctaaa agcttgctgg ctacttctac ttgctcgata actttttcgg ctttcccctg
     4441 ctccaaggct tcgaccaaag aaaccaccgt ttcttttgag gaacttaaaa aattttgatt
     4501 gatattttgc ttgatttgct ggaccatgtg actcgataca gccacttcct tggtccatcc
     4561 cactaagaaa tcacattcta aagttggttt cacttgtgag ataaaaaatc cccaatcacg
     4621 ctccagaact gtcgccaagt tttcttcttc taaccaagca gccaccttct ggcgatcaaa
     4681 tgactggtag agaaccaaat cctctgccac aatacaggca aggtcgccca tggaaccatt
     4741 gtctcctcgc ttgagcaaga cagcgctagt cagcttgaac aagagattct gatcaaccga
     4801 aagattatag agagccagta aagccttgac aaccaagaca acgacgctgc cactagaacc
     4861 tagaccaaac tttttccctt ctcgttccat tttgccacag atttttaggg aaaaaggtct
     4921 taaattctga ccgcgaacag cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa
     4981 gctgtagtca ggattaggcc ttaagtccac tgcgaaatca aacatatctg aatagatacg
     5041 gtagctgtca gaaaaagcaa tttcagccct catatagatg ggaatatcct ttatcaaagc
     5101 taactgccct ggctctaaaa tagcatattc acctgcccaa tagagttttc cgcaagtttt
     5161 aacagcaatc atcttgactc aaatcctttg tttttgacac aatcaagcga taacgctgac
     5221 cgaaaatttc tgataaatgc tccaagtctt tctcctgaca gaagacctta acattgggac
     5281 cagcatccat ggtaaagtag caggcctctc ctttctcacg aagctggcga acaaagtcca
     5341 tagcctcata agaggcatcc gtcagataag aaaaggctgg actagcagtc tttgtcgtag
     5401 catgcatagc cagggcattt ttctccgtta attctccaat cttggcaaaa tcattttcct
     5461 tgagataaat cagcatatcc tgatagtcct tctcagactg acgaacccag tcgtcgaaag
     5521 tcgtcgaggt ttccacacaa agtttcatcc cgtcacggct agagattggt tttttcttgt
     5581 cctctagcac caacataatc atagctagtt tcaagtctgt ctctacaggg taaatttctc
     5641 cactatcctt atcccaggct cctagtggtc cataaaaact ccgagaagaa gagcctgagg
     5701 caaacttggc ttcctgcgct aactgacttc tatccaatcc aagcttgaaa taagcattac
     5761 aagccttgac cagggcggac aaaccactag aacttgagga caggcccgct gccgtaggca
     5821 tattgttttg agtatcgata cggacaaagc cctcaccagc tggacgataa cggtcaataa
     5881 tcttactcat cttggcatgc tcgacctcat tttgtagctg accattgatg taaaattcgt
     5941 cagctgttac attggctggt aaaggcgaca aggtcgtctc tgtatacata ttttccaaag
     6001 ttagagaaat actgctagta gcaggcacca tctctttttc ttttttcttt ccccaatatt
     6061 tgataatagc aatatttgcg taggaacgta ctgttacagg ctctctatcc atgtctgaac
     6121 agctcctttc tcttctaatc tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa
     6181 ggctatgata caacctccta gcccaccacc gctcatcttg gcacccagag caccatggct
     6241 aagagtcgtt tcaaccaaaa agtctgcctc agggctactg actccaattt cttttaaatg
     6301 taaatgcgct tgactgagga tttgtcccag tccttcagca tctttttgtg aaatcgcaac
     6361 ttctgcttgc tgggttaatt ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt
     6421 attttgaacc acttggatgg cttcacgagt atgaccataa acacccgtat cggcaatcac
     6481 caaataggcg gataaatcca tctcaagttc tgtaaatcct acgttcttga taaagcgaat
     6541 aggttggtca ctgagacagg tcttagcatc caaaccacta ggattcatat gggcaatcat
     6601 ttcagctcga ttgaccaaga tttctagtac atcatgaggc agatcagcct gatagtagtc
     6661 aaataccgca cgaatggccg ctatgctgat agccgctgac gaacccatcc cccgtttctc
     6721 agggatagcc gagtcaatct cacaacgaat gcaggcttct gtgatatcca aatactccag
     6781 tgaggcataa accgccatgg acaaggtatc ctcctcataa aggcgccaag gactctctgc
     6841 aggaactacc ttacaggtca cctccacctc caaaagaggc agggaaatgg caggataacc
     6901 gtaaacgacc gcatgttccc ctattaaaat tatcttacta tgtgcctgac cgacaccaac
     6961 tttttttgtc attttttcct tttactagac gaaaaaacgt cttatttttc atacaagtat
     7021 taattctttc ctatctattt tattatattt tcacaaaaaa agcgattgtt tccattcaca
     7081 atcgcttctt tcattaccga acccattcac cattataatt gacagaatag ccatctacag
     7141 tcgtattcac tgccaaggca cctgagctat aagcgtagta ccatctgcca ttgacctgga
     7201 accaacctgt cgtcatagaa cgatgaaaga aactccatac cattaagtaa agaggaaagt
     7261 catgagggag catgcgccat tgacaacctg ttttagtgac gtacaaagtt tcattaacaa
     7321 gtactcgttt cggccattta taggtgcggt gtttgcagaa atagggttca atcttcgccc
     7381 attcttgatc gtttaaatca gtatcatatg ctttgcgtgt cataactcta gtttaacatt
     7441 tttttgtgaa tacaggttct aaataatcga ccacgaaaat ttcttaagtg gaaaacgcct
     7501 tatgaagtat gctacgggaa acttatgcac ttaatttgac aactcaatat gtaaaaatat
     7561 atactata
//
LOCUS       DAVLTM010000091         7460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLTM010000091 DAVLTM010000000
VERSION     DAVLTM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.092"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            418..1038
                     /locus_tag="VYN16_001647"
     CDS             418..1038
                     /locus_tag="VYN16_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404153.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1124..1567
                     /gene="tsaE"
                     /locus_tag="VYN16_001648"
     CDS             1124..1567
                     /gene="tsaE"
                     /locus_tag="VYN16_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1404154.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            1557..2075
                     /locus_tag="VYN16_001649"
     CDS             1557..2075
                     /locus_tag="VYN16_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404155.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2083..3099
                     /locus_tag="VYN16_001650"
     CDS             2083..3099
                     /locus_tag="VYN16_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1404156.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3184..4440
                     /locus_tag="VYN16_001651"
     CDS             3184..4440
                     /locus_tag="VYN16_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1404157.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGNKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4495..5661
                     /gene="recA"
                     /locus_tag="VYN16_001652"
     CDS             4495..5661
                     /gene="recA"
                     /locus_tag="VYN16_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1404158.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            5968..7338
                     /locus_tag="VYN16_001653"
     CDS             5968..7338
                     /locus_tag="VYN16_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1404159.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWVVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         2203 a         1275 c         1690 g         2292 t
ORIGIN      
        1 tagaaaatta tttgaaggaa ttagatactg tttagttaat cttgacatta tagtttttct
       61 gaaacgttaa ataacctgta aggctgatag ttagattaat actatcagcc ttatcattat
      121 gtgactctga gtccgtctcg ctccgtgagg tgcgagacaa aacaaccacc cgtttgacgg
      181 attttcatga tttcaaattt atattcttcg aaaatctctt caaaccacgt cagctctatc
      241 tgcaatctca aaacactgtt ttgagcaacc tgtggctagc tacctagttt gatctttgat
      301 tttcattgag tattagtctc tagtgtttgt ttagcgtctt aaagagacat ttgagaagat
      361 tagaaacagt tctctctttt ctcctagtat aatgattggt aggaagggag agagaagatg
      421 aaatcaatga gaatcttatt tttgttagct ttaattcaaa tcagtttgag tagctgtttc
      481 ctatggaagg aatgcatctt gtcctttaaa caaagtacag cttttttcat cggaagcatg
      541 gttttcgttt caggaatctg tgctggagta aattatcttt atacccgtaa gcaagaagtc
      601 catagtgtcc tagccagtaa gaagtcggtg aagctttttt acagtatgtt actcttaatt
      661 aatttgttag gagctgttct tgttttgtca gataacttgt tcatcaaaaa tacgctgcag
      721 caagaattag ttgacttttt attgccatcc ttctttttcc tatttgggct agatttgctg
      781 atttttttac ccttgaaaaa atacgtgcgc gattttcttg ctatgctaga cagaaaaaag
      841 acagtgttgg tgactatttt agcaacactt cttttcttaa gaaatccaat gaccattgtc
      901 tcacttctga tttatactgg actgggcttg ttttttgcag cctatcttgt cccaaattcg
      961 gttaagaagg aagtttcctt ttatggtcat attttccgag atcttgtatt ggtcattgtt
     1021 acgctcattt tcttttagag aaagcctgtt ttttatggat gttatgatga taagtttagt
     1081 aggaaaatag acacaaaaga aaagtttttg gtataataag agtatgtaca caaaaaatga
     1141 agaagagttg caagccttag gggagcgttt gggccatcta ttagcaaaga atgatgtttt
     1201 aatcttaatt ggagaactgg gtgcaggtaa aacgaccttt actaaaggac ttgcaaaagg
     1261 attacagatt tctcaaatga ttaaaagtcc tacctatact atcgtgagag agtatgaagg
     1321 tcgacttcca ctttatcacc tagatgttta tcgtattgaa ggagatgctg attctatcga
     1381 cttggatgag tttatctttg gtggcggcgt gactgttatt gagtggggaa atctcttagg
     1441 agatgccttg ccagatgctt atttagaatt ggaaattcta aaagaagcag atggacgccg
     1501 tttaaatttt caggcaaagg gtttgcgtgc tgagaaattg ttagaggagc ttcaatatgg
     1561 agtatgaatt gctcattagg gaagcagagc ccaaagatgc agctgaatta gtggcctttt
     1621 taaatcgtgt gagtttggag acagacttta ccagcctaga cggagatggt attctcttga
     1681 ctagtgagga gatggaaata ttcctcaaca agcaagctag ttcggacaat cagataacct
     1741 tacttgcatt cttaaatggc aaaattgctg gtattgtaaa tattacagct gaccagcgta
     1801 agagagtccg tcatattgga gatctcttca ttgtgattgg aaaaagatat tggaataatg
     1861 gcttgggaag tttgttgcta gaagaagcga tagagtgggc acaagcaagt ggcattctgc
     1921 gtcgtctcca actgactgtc caaactcgta atcaagcagc agtccatctt tatcaaaagc
     1981 atggctttgt cattgaaggt agccaagagc gtggggcata tatagaagaa gggaaattta
     2041 tcgatgttta cttgatgggt aaactgatag attagtagaa atatggttaa aaaaattatt
     2101 ggaatggtgc tagctttact ttctgtaact gtagtaggag taggtgtttt tgcttatact
     2161 atttatcaac aagggacaga aaccttagct aaaacctata aaaaaatcgg cgaagaaacc
     2221 aaggttattg aagcgactga acctctaacc attctgttaa tgggagtgga caccggaaat
     2281 gctgaacgaa ctgaaacttg ggtcggtaga agtgatagca tgatcttgat gacagtgaat
     2341 cctaaaacga aaaaaacaac aatgatgagt ttagagcggg atattctgac gcgcattgaa
     2401 tcagggaatg gtcaggctca tgaagcgaaa ctgaactcag catatgcaga tggtggagca
     2461 gagcttgcta tagaaaccat tcaaaaaatg atgaatatcc atattgatcg ctatgtgatg
     2521 gtcaatatga gagggttgca aaaactagtg gatgcagtag gaggtattac agtcaataat
     2581 atcctaggtt tcccaatttc tatcagtgac caagaagaat ttaatactat ttctatcggt
     2641 gttggggagc aacatattgg gggagaagaa gccctagtct atgcacgaat gcgttaccaa
     2701 gatcctgagg gggattatgg tcgtcaaaaa cgtcaacgtg aagttattca aaaagtcatg
     2761 gaaaaagctc tcagtttaaa tagcgttggt cattatcaag agattctaaa agctttgagt
     2821 gacaatatgc agaccaatat tgatttgtct gcaaaaagta tccctaactt gctaggctat
     2881 aaagattcat ttaaaaccat tgaaactcag cagttgcagg gtgaaggaga gatacttcaa
     2941 ggtgtttctt accagattgt ttcgagagca catatgttgg aaatgcaaaa tatactccga
     3001 cgttctttgg gacaagaaga agttactcag cttgaaacca atgcggtttt atttgaagat
     3061 ttatttggca gagcacctgt tggtgatgaa gataattaac ttttgataca aataaaaaaa
     3121 tcaatcgtag gaaaattcct gcgatttttt caaaaaaata cgaatagata ggtaggagga
     3181 aacatgaaag cagaaatcat tgctgttgga acagagattt tgacaggaca gattgtcaac
     3241 accaatgccc agtttttgtc ggaaaaacta gctgagattg gggtagatgt atattttcag
     3301 acggctgtag gagacaatga agttcgtctc ttgtctttgc ttgagattgc cagtcaacgt
     3361 agcagtctgg tgattttgac aggcggtttg gggccaactg aggacgacct aaccaaacaa
     3421 accctagcta aatttttagg gaaagcatta gtctttgatc ctcaggctca ggagaagttg
     3481 gatatctttt ttgccctgcg accagactat gcccgaacac cgaataacga aagacaagct
     3541 caaattgtag aaggagcgat tccactgcca aacgaaacag gactggctgt tggaggaaaa
     3601 ttagaagtag acggagtgac ctatgtcgtc cttccaggtc cgccaagtga attgaaaccc
     3661 atggtcttaa accaacttct acccaagttg atgacaggga acaagctgta ttcccgagtt
     3721 cttcgtttct ttgggattgg cgagagccag ttggttacga ttttggctga tttaattgat
     3781 aatcagatcg atcctacctt ggccccttat gccaagacag gagaagtcac tctacgtctg
     3841 tcaacaaagg ctagcagtca agaagaggcg aatcaagcgc tggatatctt ggaaaatcaa
     3901 atcttggact gccagacttt cgaaggaatt tctttacgag acttttgcta tggttatggg
     3961 gaagaaacta gtttagccag cattgtggta gaagaactga aaaggcaagg gaaaaccatc
     4021 gcggctgcag agagtttgac ggcaggtctt ttccaagcta ccgtggcgaa tttttctgga
     4081 gtttcaagta tatttgaggg tggttttgtg acctatagct tggaggaaaa atcaaggatg
     4141 ttggatattc ctgccaagaa tttggaagaa catggtgtgg tgtctgaatt tacagctcag
     4201 aagatggctg agcaggcacg aagcaagacc cagtctgatt ttggaattag tttgactgga
     4261 gtggcaggac cagatagcct agaaggacac ccagttggga cagtcttcat aggcttggcg
     4321 caagatcaag gaactgaggt tatcaaggtt aatattggag gcagaagccg agcagatgta
     4381 cgtcacattg cggttatgca tgcctttaac ctagttcgca aggctttatt aagtgactaa
     4441 cttttgatat aatagtagat aggtctgagg atcattagaa tgtaggagaa tagaatggcg
     4501 aaaaaaccaa aaaaattaga agaaatttca aaaaaatttg gggcagaacg tgaaaaggcc
     4561 ttgaatgacg ctcttaaatt gattgagaaa gactttggta aaggatcaat catgcgtttg
     4621 ggtgaacgtg cggagcaaaa ggtgcaagtg atgagctcag gttctttagc tcttgacatt
     4681 gcccttggct caggtggtta tcctaaggga cgtatcatcg aaatctatgg cccagagtca
     4741 tctggtaaga caacggttgc ccttcatgca gttgcacaag cgcaaaaaga aggtgggatt
     4801 gctgccttta tcgatgcgga acatgccctt gatccagctt atgctgcggc ccttggtgtc
     4861 aatattgacg aattgctctt gtctcaacca gactcaggag agcaaggtct tgagattgcg
     4921 ggaaaattga ttgactcagg tgcagttgat cttgtcgtag tcgactcagt tgctgccctt
     4981 gttcctcgtg cggaaattga tggagatatc ggagatagcc atgttggttt gcaggctcgt
     5041 atgatgagcc aggccatgcg taaacttggc gcctctatca ataaaaccaa aacaattgcc
     5101 atttttatca accaattgcg tgaaaaagtt ggagtgatgt ttggaaatcc agaaacaaca
     5161 ccgggcggac gtgctttgaa attctatgct tcagtccgct tggatgttcg tggtaataca
     5221 caaattaagg gaactggtga tcaaaaagaa accaatgtcg gtaaagaaac taagattaag
     5281 gttgtaaaaa ataaggtagc tccaccgttt aaggaagccg tagttgaaat tatgtacgga
     5341 gaaggaattt ctaagactgg tgagcttttg aagattgcaa gcgatttgga tattatcaaa
     5401 aaagcagggg cttggtattc ttacaaagat gaaaaaattg ggcaaggttc tgagaatgct
     5461 aagaaatact tggcagagca cccagaaatc tttgatgaaa ttgataagca agtccgttct
     5521 aaatttggct tgattgatgg agaagaagtt tcagaacaag atactgaaaa caaaaaagat
     5581 gagccaaaga aagaagaagc agtgaatgaa gaagttccgc ttgacttagg cgatgaactt
     5641 gaaatcgaaa ttgaagaata agctgttaaa gcagtggaga aatccgctac tttttcgatt
     5701 tttgattcaa gtttttagat tatatatagt agcttgaaat aagatatgaa caactttatt
     5761 aggaaagtca aattaatttc tagaaatgtt ttagcagcta cagcgtacta ttccaaactc
     5821 aaccaactat aatagatcga aactagaata gtacacatct acttctaaaa cattgttaaa
     5881 aatcgatttg actgtcctta tttcattccg ctatatataa gtttgctgtt tcttgtcgct
     5941 cctctggaaa gctgatataa tagctttatg aataaaaaac gaacagtgga cctgatacat
     6001 ggtccgattc ttccctcgct cttaagcttc acctttccaa tcttgctatc aaatattttt
     6061 caacagctct ataacactgc tgatgtcttg attgttggac gatttcttgg tcaagaatcc
     6121 ttggctgcag taggagcgac gacagcgatt tttgacctga ttgtaggctt tacacttggt
     6181 gttggcaatg gcatggggat tgtcattgct cgttattatg gggctcggaa tttcactaaa
     6241 atcaaggaag cagtagcagc cacctggatt ttaggtgctc ttttgagcat tctagttatg
     6301 ttgctgggct ttcttggctt gtatcctctc ttgcaatact tagatactcc tgcagaaatt
     6361 cttcctcaat cttatcaata tatttctatg attgtgacct gtgtaggtgt cagctttgct
     6421 tataatcttt ttgcaggctt gttgcggtct attggtgaca gtctagcagc cctgggattt
     6481 ctgattttct ctgccttggt taatgtggtt ctggatctct attttattac gcaattgcat
     6541 ctgggagttc aatccgcagg acttgctacc attatttcgc aaggtttatc agcggttctc
     6601 tgcttttatt atattcgtaa aagtgtgcca gaactcttgc cacagtttaa acatttcaaa
     6661 tgggacaaaa gcttgtacgc ggatctcttg gagcaaggtt tggctatggg cttgatgagt
     6721 tcaattgtat ctatcggcag tgtgatttta cagttttctg ttaatacatt tggtgcagtg
     6781 attattagtg cccagacggc agctcgacgc attatgacct ttgcccttct tcctatgacc
     6841 gctatttctg catcaatgac gacctttgct tctcagaatc taggagctaa gcgacctgac
     6901 cgtattgttc aaggtcttcg aatcggcagt cgtttaagta tatcctgggt agtttttgtt
     6961 tgtattttcc tcttttttgc cagtccagct ttggtttcct tcttggctag ttcgacagat
     7021 ggttacttga tagaaaatgg aagtctctat ctgcaaatca gttcaacctt ttatcccatt
     7081 ttgagcctct tgttgattta tcgcaattgc ttgcagggct tggggcaaaa gatccttcct
     7141 ctagtttcta gctttattga actaatcgga aaaatcgttt ttgtggtttt gattattcct
     7201 tgggcaggat ataagggtgt tatcctttgt gaacctctta tctgggttgc catgacagtt
     7261 caactgtact tctcattatt ccgtcatccc ttgataaaag aaggcaaggc aatcttggca
     7321 accaaagtgc aatcctagtt ggatttactg aataaaatcc atttcctcta gtgaaaatcg
     7381 aaaaaacttg tgttctcgtc tttagtttgg tgttgaaaat agtttaacag acttttgact
     7441 tcttttatat gatataataa
//
LOCUS       DAVLTM010000092         7374 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLTM010000092 DAVLTM010000000
VERSION     DAVLTM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.093"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            44..199
                     /locus_tag="VYN16_001654"
                     /pseudo
     CDS             44..199
                     /locus_tag="VYN16_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            399..3674
                     /gene="rexB"
                     /locus_tag="VYN16_001655"
     CDS             399..3674
                     /gene="rexB"
                     /locus_tag="VYN16_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1404160.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKENSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3671..7321
                     /gene="addA"
                     /locus_tag="VYN16_001656"
     CDS             3671..7321
                     /gene="addA"
                     /locus_tag="VYN16_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1404161.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILNQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQKGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALKMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2244 a         1564 c         1570 g         1996 t
ORIGIN      
        1 ctattatagt ttcaatttac tatagttcaa tcattctatc aagaatattg aatatataaa
       61 ggcacgtaat attaaaactt cttctgatat cgaattagag tccgatgttg atggcgataa
      121 atcagacaat ctaccagtcg acattaagat actgggacaa cacattgaag tgtattccgt
      181 acctaagaaa caatcataaa actcaaagcc ttgttaccga ggctttttta ttatgtgatt
      241 ttcagtagcc agacttattc tatctaatac aagtaaagct aagtatttaa gatttagcag
      301 atgatgaaga aaagaaagga tggcctaaat attttaacag atttacgagg ctaatcatgg
      361 tataatatag ggtgatgaaa aattccaacg aggctgagat gaaattactt tatactgata
      421 ttcggacttc tttgacagaa attctaacaa gagaggcaga agagctagtt gcagctggca
      481 agcgggtctt ctacattgcc cccaactctc tttcttttga aaaggaacgc gccgtgctgg
      541 aatgcttgtc ccagcaggct tctttttcga ttaccgtcac gcgctttgct caaatggctc
      601 gttatctggt cttgaatgat ttaccagcta aaactactct tgatgatatc ggtcttgggt
      661 tggcctttta caaatgcctt gccgaactcg atcccaagga cttgcgtgtt tatggcgcta
      721 ttaagcagga tcctcaattg atccagcagt taattgagct ttaccatgag atgaccaaat
      781 cccagatgag ttttttggac ttggagaatt taacagatga ggataagagg gcggatttac
      841 tcttgatttt tgagaaagta acagcctatc ttaatcaagg tcagttagcc cagggaagtc
      901 agttgtccca tttgattgag gctattgaga atgacaaggt aagtagtgat tttaatcaaa
      961 tcaccttggt cattgacggc tttactcgtt tttctgctga ggaagagcgg gttgtggact
     1021 tacttcacgg caaaggtgtt gagattgtta tcggggctta tgctagtaag aaagcctata
     1081 ccagtccttt tagcgagggc aatctctacc aagccagcgt aaaatttctc catcatctgg
     1141 cttctaaata ccaaacgcct gctcaggact gttctcaaac tcatgagaag atggatagtt
     1201 ttgacaaggc ctctcgtttg ttggagtctt cttatgactt ttcagaactc gctttggatg
     1261 tcgatgagaa agaccgtgaa aatttacaaa tctggtcttg tttgacgcaa aaggaggagt
     1321 tggagctagt agcccgtagt attcgtcaga aattacatga gaactcagac ctgagctaca
     1381 agcattttcg tattctcttg ggggatgtag cttcttacca gttatctctc aaaaccattt
     1441 ttgaccagta tcagattcct ttttatcttg gtagaagcga agccatggct catcatccct
     1501 tgactcagtt tgtcgagtct attttagctt taaaacgtta ccgttttcgt caggaggatt
     1561 tgattaatct tcttagaact gatttgtata ctgacctcag tcagtctgat attgatgctt
     1621 ttgagcaata tatccgctat cttggtatca atggcttgcc agcctttcag caaaccttca
     1681 ccaaatccca ccatggaaaa tttaatcttg agcgtttgaa tgtcctccgc ctgagaattt
     1741 tagcacctct tgaaaccctc tttgccagcc gaaaacaaaa ggctgaaaaa ctcctacaaa
     1801 aatggagtgt ctttctaaaa gaaggagctg tgaccaagca gttacaagat ttgacaacca
     1861 ctttggaagc tgtagaacag gaaagacaag ccgaagtttg gaaggctttc tgccatgttt
     1921 tagaacaatt tgcgactgtt tttgctggtt cacaggttag tctggaagac ttcctagcct
     1981 tgctccattc tggaatgagt ttgtcccaat accgtaccat tccagcaaca gtggacactg
     2041 ttctggtgca gagttacgat ttgattgcac cattgactgc tgactttgtc tatgctattg
     2101 gactaactca ggacaattta ccaaaaattt ctcaaaacac cagtcttctg acagatgaag
     2161 aaaggcaaaa tctaaaccaa gcgaccgaag aaggcgttca attactgatt gccagcagtg
     2221 aaaatctcaa gaaaaatcgc tacactatgc tttccttggt caattctgct cgtaagcagt
     2281 tgttcttgtc ggctccaagc ctttttaacg aaagtgaaag taaggaatct gcctatcttc
     2341 aagagttgat ccattttgga tttaggcgga gagagaagag gatgaatcac aaaggactgt
     2401 ctaaggagga tatggggtcc tatcacagtc ttttgtctag tctggttgcc tatcaccagc
     2461 agggtgagat gagcgatact gagcaagatt tgacttttgt caaggttctg tcgcgtgtca
     2521 taggtaaaaa actagatcag caaggtctgg aaaatccagc catcccaatc agtccaagca
     2581 gcaagacctt agccaaggac accttgcaag ctctctatcc tgccaaacag gagttttacc
     2641 tgtctacgtc gggtttgaca gagttttatc gcaatgaata cagttatttc ctacgctacg
     2701 ttttaggctt gcaggaggaa ttacgtttgc gtcctgatgc ccgtagtcac gggaatttct
     2761 tgcatcgtat ctttgaacgc gccttacagt tgcctaatga agattccttt gaccaacgtc
     2821 tagaacaagc tattcaagaa accagtcaag aacgcgaatt tgaagctatt tatcaagaaa
     2881 gtttggaagc ccagtttacc aaggaagttt tgcttgatgt tgcacggacg actggacata
     2941 ttctccgaca caatccagcc atcgaaacca tcaaagaaga agcaaatttt ggtggaaaag
     3001 accaagcctt tattcaatta gacaatggac gcagtgtctt tgtacgaggc aaggtggacc
     3061 ggattgaccg tttgaaagct aatggagcga taggagtagt agactacaaa tccagtctga
     3121 ctcagttcca gtttcctcat ttctttaatg ggctcaattc tcagttacca acctatcttg
     3181 ctgccctaaa aagagaaggg gagcagaact ttttcggcgc catgtacttg gaaatggctg
     3241 aacctgtcca atctctgatg gcggtaaaaa gtctggcagg agcagtggta gaagccagca
     3301 aatctatgaa ataccaaggg ctcttcttgg aaaaagaaaa cagttattta ggcgaatttt
     3361 ataacaaaaa caaggctaat caactgacag atgaggaatt tcagctccta ctggactaca
     3421 atgcctatct ttacaagaaa gctgctgaga agattttagc aggccggttc gccatcaatc
     3481 cttatactga aaatggcaga agcattgccc catacgtcca gcaacatcag gctattacag
     3541 gctttgaagc caattaccat ctgggccaag cccgtttcct aaaaaagttg gacctagctg
     3601 atggcaagcg tctggtcgga gaaaaactca agcaagcttg gtttgaaaaa ataagagagg
     3661 agttaaatcg atgaagctta ttcccttttt aagtgaggag gagattcaaa aactgcaaga
     3721 agcagaagca aattcgagca aggaacaaaa gaaaactgcc gagcaaatcg aagctatcta
     3781 cacttctggc cagaatatcc tggtctcagc atcggctggt tctggaaaga cctttgtcat
     3841 ggcagagcgc attctgaacc aattggcgcg tggtgtcgaa atttctcaac tctttatctc
     3901 aacctttacc gtcaaggctg caactgaact taaagaacgt ttagagaaaa aaatcagcaa
     3961 gaaaattcaa gaaacagatg atgtcgacct caaacaacac ttgggacgcc agttggcaga
     4021 cttaccaaac gctgccattg gaaccatgga ctctttcaca caaaaattcc ttggcaaaca
     4081 tggttatctg cttgatattg cacctaattt ccgtatttta caaaaccaaa gcgagcaact
     4141 tcttctcgaa aacgaagtct ttcatgaggt ctttgaagcg cattaccaag gtaaacagaa
     4201 agagaccttt agtcatttgc tgaaaaactt tgctgggcgt ggcaaggacg aacggggtct
     4261 gcgccagcag gtctataaaa tctatgactt cctccaatcc accagtaatc ctcaaaagtg
     4321 gttgagtgaa tctttcctca aaggctttga gaaagctgat tttaccagtg aaaaagaaaa
     4381 actgactgag caaatcaaac aagccctttg ggatttggaa agctttttcc gttaccatct
     4441 ggataacgat gccaaggagt ttgcaaaggc tgcctattta gaaaatgttc agttaattct
     4501 ggatgaaatt ggctccctaa atcaggagtc cgatagtcag gcttatcagg cagtgcttgc
     4561 gcgtgttgtc gccatctcta aggagaaaaa cggtcgagct ctgactaatg ccagtcgtaa
     4621 ggctgatttg aagcccctgg ctgatgccta caacgaagag agaaagaccc agtttgctaa
     4681 actaggacaa ttatcagacc agataaccat tctcgactat caagaacgtt atcatcaaga
     4741 cacatgggaa ctagctaaaa ccttccaaac ctttatgagt cattttgtgg aggcttatcg
     4801 tcagcgcaaa cgccaggaaa atgcctttga attcgctgat atcagccatt acaccattga
     4861 gattttagag aatttcccac aagttcgtga gtcttatcag gagcgcttcc atgaagtcat
     4921 ggtcgatgag tatcaggata ccaaccatat tcaagaacgg atgctggaat tgttgtctaa
     4981 tggccacaat cgctttatgg tgggagatat caagcaatcc atctatcgtt tccgtcaggc
     5041 agacccgcag attttcaatg agaaattcca acgctatgcg caaaatcccc aaaaaggcaa
     5101 gctcattctc ctcaaggaaa atttccgtag tagttcagaa gtgctgtcag caaccaatga
     5161 tgtctttgaa cgtctcatgg accaagaggt cggcgaaatc aactatgata acaagcacca
     5221 gcttgttttt gccaatacca aactgactcc caatccagac aacaaggcag aatttctcct
     5281 ctacgacaag gacgatacag gtgaggaaga agagagtcaa acagaaacga aactaacagg
     5341 cgaaatgcgc ttagttatca aggagattct gaaacttcat caagaaaaag gtgttgcctt
     5401 taaggaaatt gcccttctga cctccagccg cagtcgtaat gaccagattc tcctcgccct
     5461 gtctgagtac ggaattcctg tcaaaactga cggagagcaa aacaattatc tccaatccct
     5521 agaagtgcaa gtcatgctag acactcttcg tgtcattcac aatcccctgc aagactacgc
     5581 cttggttgcc cttatgaagt ctccaatgtt tggttttgat gaggatgagc tagcacgttt
     5641 gtcccttcag aaagcagagg ataaagtcca cgaaaatctc tatgaaaaac tggtcaatgc
     5701 acaaaaaatg gcaagtagtc aaaaaggttt gattcacaca gctctagctg aaaaactaaa
     5761 gcaattcatg gatatcctag cttcttggcg cttgtatgcc aaaacccact ctctctatga
     5821 cttgatttgg aagatttaca acgaccgttt ttattatgac tatgttgggg ctttgccgaa
     5881 tggtcctgct aggcaggcca atctctatgc cctagcactg cgtgctgatc aatttgaaaa
     5941 gagcaatttc aaaggtttgt cgcgttttat tcgtatgatt gaccaagtct tagaagccca
     6001 gcacgatttg gcaagcgtgg ccgtcgcacc gccaaaagat gcagtagagc tcatgaccat
     6061 ccacaagagt aaggggctgg agtttcctta cgtctttatc ctcaatatgg atcaagattt
     6121 caacaagcaa gactctatgt cagaagtcat tctcagtcgt cagaatggtc ttggtgtcaa
     6181 atatattgcc aagatggaga caggggcagt agaagaccac tatcctaaaa ccatcaaact
     6241 ctccattcct agcctgacct ataggcagaa tgaagaggaa ttacagctag caagctattc
     6301 tgagcagatg cgtttgctgt atgttgctat gacgcgggct gagaaaaagc tctatcttgt
     6361 cggcaagggt tctcgtgaaa agctggaagc caagcaatac ccagcagcca aaaatgggaa
     6421 actaaatagc aatactagac tgcaagcacg gaatttccaa gattggcttt gggctatcag
     6481 taaagtgttt actaaggaca agctcaactt tagttatcgt tttattggcg aagatcagtt
     6541 gaccagagaa gctatcggag agttggaaac caagagtcct ctccaagata gctcccaagc
     6601 agacaatcgt cagtcagata ccatcaaaga agctctgaaa atgctgaagg aggtggaagt
     6661 ttataatact cttcaccgcg cagctattga acttcctagt gttcaaaccc caagtcaaat
     6721 caagaaattc tacgaaccag ttatggatat ggaaggtgtc gagattgctg gtcaaggtca
     6781 gtcagtaggc aagaaaatca gcttcgattt gccagatttt tcaacaaaag aaaaggtaac
     6841 tggagctgag attggtagtg ctactcacga actcatgcag agaattgacc tcagccagca
     6901 actaaccctt gctagcctaa cagaaacact caaacaagtt caaactagcc aagctgtcag
     6961 agacaagatc aatcttgata aaattcttgc tttctttgac acagtactcg gtcaggaaat
     7021 tcttgctaat accgaccatc tctatcgcga gcaacctttc tccatgctca aacgagacca
     7081 aaagagtcag gaagactttg ttgtccgtgg tatccttgat ggctatctgc tttacgaaaa
     7141 caaaattgtt ctgttcgact acaagacaga ccgctatgat gaaccaagtc aactcgtaga
     7201 ccgctatcgt ggtcagttag ctctatacga agaggcttta tcacgagcct attcgattga
     7261 aaatattgaa aaatacttga ttttactcgg taaagacgag gttcaagttg taaaagtata
     7321 acttatactc tttgaaaatc tcttcaaacc acgtcagctt cgccttgctg tata
//
LOCUS       DAVLTM010000093         7303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLTM010000093 DAVLTM010000000
VERSION     DAVLTM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.094"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(135..2117)
                     /locus_tag="VYN16_001657"
     CDS             complement(135..2117)
                     /locus_tag="VYN16_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1404162.1"
                     /translation="MKSSRLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGASEPILYKYLELQ
                     DKAITVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2363..3217
                     /gene="rfbD"
                     /locus_tag="VYN16_001658"
     CDS             2363..3217
                     /gene="rfbD"
                     /locus_tag="VYN16_001658"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1404163.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
     gene            complement(3279..4325)
                     /gene="rfbB"
                     /locus_tag="VYN16_001659"
     CDS             complement(3279..4325)
                     /gene="rfbB"
                     /locus_tag="VYN16_001659"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1404164.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(4337..4930)
                     /locus_tag="VYN16_001660"
     CDS             complement(4337..4930)
                     /locus_tag="VYN16_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1404165.1"
                     /translation="MSDNFFGKTLAVRKVDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFVAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            complement(4931..5800)
                     /gene="rfbA"
                     /locus_tag="VYN16_001661"
     CDS             complement(4931..5800)
                     /gene="rfbA"
                     /locus_tag="VYN16_001661"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1404166.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAQKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PENPRSNYAVTGLYFYDNDVVEIAKGIKPSARGELEITDINKVYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(5941..6768)
                     /locus_tag="VYN16_001662"
     CDS             complement(5941..6768)
                     /locus_tag="VYN16_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV1404167.1"
                     /translation="MINKVDYFGMDISDLKNKKLFLFDMDGTIYEEDRLFDGTLQLLE
                     YIQSIGGEYIFITNNSSKSVVDYIEKVNQLGIVAKRENFFTSAQATIVFIEEKYPHAK
                     VYCQGTKSLLKELSDSGIDVTDQVRDNIDLVLVGFDTELTSEKIRNTCEVLSTRDVPF
                     IATNPDVRCPVSFGFLPDCGSICEMISKSVDKTPIYIGKPEPTMVNIVRKKLNYSLSE
                     TVVIGDRLYTDIMTGINAGVSSVCVLTGEATADDIQQGSIKPTYTFRTVKEMWEGII"
     gene            complement(6789..>7303)
                     /locus_tag="VYN16_001663"
     CDS             complement(6789..>7303)
                     /locus_tag="VYN16_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEV1404168.1"
                     /translation="SGKYPEIKIIDNVDYKITNNMYSAYLGKDAMGSSEFLMMNADVF
                     YDASVIKSLLLHEAPNAIVTDLGTYIDESMKVVEKNGRLVEISKQISPEEALGASIDV
                     YKFSPDAGACFFEKCREFIEDKNELQLWSEVALNAILQAVEFVACPLDGRWLEIDNHE
                     DLAAAEKLFA"
BASE COUNT         2147 a         1599 c         1311 g         2246 t
ORIGIN      
        1 aaaaattttc tctattctga caaaaagagg ctccaacttt tgggggtcag tacatttaga
       61 atctttcttt acaaatataa aaaggattca gcattcaaga gaaatactaa atcctttctt
      121 atattttgca acagttattt cacatgtttt gcgagatctt cttgagcctt tttattagac
      181 tcttcttttt ctttcatcca tttttcctga gctttttggt attcatctac agtgattgcc
      241 ttgtcttgaa gttccaagta tttatacaag attggttcac ttgcaccttt atttcctgac
      301 aatgcaaatg gtattgtaaa tggtaccatc ttagacaaga ttgggcgccc tgtacgagat
      361 gtagttggaa taatcaaagc actatctgtc aaccaagctt gggctgcagc gtatttatca
      421 tagcgtttag caacatctgt agcctcatca ccagcctcag tgaccaattt ttcgtagtca
      481 tatagaccta cttttttagc agctacatta tcttcccctg agtcaaaccc taaatatgtt
      541 ttagtacttt ctcctacaga tggtttgatg atatcaaggt aggttgatgg atcggcaaag
      601 tctggacccc aaccgacatt atctgataaa tcccagtctt cgccagcagc attttcagca
      661 aaatatgtaa tattgtttac ttcgtctttt tgtagttgtt ggatatcaat aatgacatta
      721 tcagctccta aagttgcttc caaggattgt ttcatagatt ggacgcgctg aacttttgta
      781 gttgctgtct ggtcaactgg catatccaaa tgaattggga aagtcacacc ttctgcttgt
      841 aaggctgatt tagctttagc aaattcagcc ttggcttttt ctggattgta aagaccatcc
      901 tgagaatctg caagattaac atccttccat tcatccccat aagtgaccaa tttctctttg
      961 accatatcgc caaagttttt accatctgct tgaacaaatg ttggtggcac aaagatatta
     1021 cgtaagattt tgcttgctcc agtttgtcca ttcaactgag aggcataggc tgtacgatca
     1081 aaaccaaagg caatagcctg acggaaatcc ttgtttaaga gagccttttt agtcgatgcc
     1141 ttttgttcat cgctggtctt agatgtgtat ttataggact gacggtcaat atttgtacca
     1201 actagatacg taatagagtc ttgttgagta tagacaatat tgtccttcat actcttctca
     1261 agctctgcga aacttgcact tgttggatag agacgagctg ctgtaaggct accatcttta
     1321 aagttttctg caggtttgct ggtatcttga ccatcccaga atgacaattt aactttgtca
     1381 acatgcacat tgtccttatc ccagtagttc ggatttttcg caaattcaac agaggatttg
     1441 gtcacaatgg atttcaacaa ataaggaccg ttatacaaga gactacttgg atccgtagct
     1501 ttggcaaaat catctccttt tgaattcaaa aactcttcat taactggcgc aagcacaccc
     1561 atggttgtct tagaattcca gaagctttct ggtttgttca aagtgtactg aactgtctgt
     1621 tcatccagag ccttaattcc tacttgtgag aaatctttga tttccccttt tacataggca
     1681 tccaaccctt tgattgattc ttgaacaagg taaagagcat ctgatttttt atcagcagca
     1741 tattttaatc ctgttacaaa gtcttgagct ttgactgccg cgtattcttc accttcagaa
     1801 gtataccatt ttgcatcctt acggatagta taagtgtaag tcaatccatc cttggataca
     1861 gaccaatcct cagccataga cggcacaaag ttcccgtagc gatcattttc tagcaaacca
     1921 tcaaccacgt tactggtaat atttgctgtc gcagccttag cagttgtcaa atagttgagg
     1981 ttatcagggt ctgtctcata aatgtatgag aatgtcttct cacctttagt gcttgaacct
     2041 gatccagagc atgcagctaa agtagtcgcc gccaataatg tcacgcccgc aagggcaagt
     2101 agtcttgaac ttttcatcat tttctccttt aaaactttct ctctattata gactcttttc
     2161 agagagatgt caatataatt ttctgaattt tttaattaat ttggtaaatt ctgaaaaata
     2221 cttttaagga tattctaaag taataactat tcggctctct tgcgcttcac taacaaagtt
     2281 tataccaaaa aagggttcac taaaaaccga tttttctata tttttgatat aattaagtat
     2341 aagaactatc aaggagaaaa atatgatctt aattacaggt gcaaatggtc aacttggtac
     2401 cgaactacgt tatttactag atgagcgaaa tgtggactat gttgcagtgg atgtgaccga
     2461 aatggatatc actaattctg aaatggttga aaaagtcttt gctgaggtta agccaactct
     2521 agtctatcat tgtgcagcct acactgctgt tgatgcagcc gaagatgaag ggaaagaact
     2581 ggattttgcc atcaacgtaa ctggaactga aaatgtagcc aaggcctctg agaaatacgg
     2641 agctattctg gtctatatct caacagacta cgtctttgat ggaaagaaac cagtaggaca
     2701 agagtgggaa gtcgatgaca tacctgatcc gcaaacagag tacggacgta ccaagcgaat
     2761 gggagaagaa cttgttgaaa accttacgtc acagcattac atcattcgta ctgcttgggt
     2821 ttttggaaat tatggaaaaa actttgtctt taccatgcaa agcctagcca aaacccataa
     2881 aacgctcaca gtggtcaacg accaatacgg tcgcccaact tggacacgga ccttggccga
     2941 attcatgacc tatgtgactg aaaatcaaaa agagtatggc tactatcact tatcaaatga
     3001 cgcagctgaa gacaccactt ggtatgactt tgctagcgag attcttaagg acagtgatgt
     3061 tgaagtagtg ccagtagact ctagcaagtt ccccgctaag gctaaacgac ctctcaactc
     3121 aactatgagt ttggcaaagg caaaggcaac aggtttcgtc atcccaacct ggcaagatgc
     3181 ccttaaagag ttttataaac aagaagtaag aaaataaaag cttactacat actagaaaag
     3241 caagagacct cgaggtctct tgctttttta gtatcttatt atttaatcac ttgttgcgtt
     3301 ttagcatagt tagcctctac tgcttctttt tcagatttcc accagtcttg gttatctgtg
     3361 taccacttga tggtctcttt gaggcctgct tcaaagttgg taaactctgg cttccacccc
     3421 aactcatcac ggagcttgct agcatcaatc gcataacgca agtcgtgacc agctcgatcg
     3481 gtcacatggt cataagcgtc agcgggttgc cccatctcct tgagaatgag ctctaacact
     3541 tccttgttgt tcttctcacc gtcagcaccg atcaagtaag tttcaccgat ttgacccttg
     3601 gtcagaatcg tccaaacgcc tgatgaatgg tcattggtgt gaatccagtc acgcacattc
     3661 ttaccttctc cgtagagttt tggcttgata ccgctcaaga tattcgtaat ctggcgcgga
     3721 atgaacttct cgatatgctg gtatggacca tagttgtttg aacagttaga aatcgttgct
     3781 ttaacaccaa atgagcgcac ccaagcttta acgatcaagt ctgaagcagc cttggttgat
     3841 gagtaaggcg agcttggatt gtacttggtt tcagcggtaa atttctcacc taggccttcc
     3901 ccatgacctg gcaaatcttc acgcagaggc aggtcaccat agacttcatc agtcgataca
     3961 tggtggaaac gaatgtcgta tttacgagcc gcttctaaaa gtgtgtaagt tccgataaag
     4021 ttggtgtgga taaatggact cgggtcattg agcgagttat cattgtggct ttctgccgca
     4081 tagtgaacga tagcatccgc ttcagccgcc aacttgtcta ccaaggctgc atcagcaata
     4141 tctccaacaa ccaactcaac acggtcgcct aaaatttcct cgatattggc acgattacct
     4201 gcataagtca gcttgtccag cactgtcaca tggacatctg gaaagttatt gtaaacatag
     4261 tggacaaagt tagaaccgat aaaaccagct ccacctgtca cgataatttt tttgtattca
     4321 gtcataatgt ttccttttac aactcgtctt tcgtaagtgg tttgacatcc cttaatagag
     4381 gatggtgttt gtctgcctct gaaacctcag cttccgctag attttcccac tcaattccca
     4441 aggatgggtc agcatagttg acaaaggcat atttgggttt tagttcaaga gcccagtagt
     4501 cattgaccag atagctatag gaaactgtat ccgagaggac ttggaaacca ttggcgacgc
     4561 cacgagggac aaaaattccc ttgctagcat caatctctgt ctggtaaaca tttccaaaag
     4621 tctcgccttc acgcaaatcc acccaagatc ctaaaacctt cccatcgtct gcaacagaga
     4681 tatacttgtc ccaaggttct gcatgcaatc ctcgaagaac attttttcga gaaaagctga
     4741 cgttgttttg caatttccct gcaacaaaga agctttcagg aaaaccaagc ggtagcatct
     4801 tttccttctg gaagttttcc ttaaaccaac cacgattgtc tccatgaacg ggaatgtcaa
     4861 actctagcag tcctggtata gcatcaacct tgcgtaccgc aagtgtcttt ccaaaaaaat
     4921 tatctgacat ctatgcttct ccaatcaaac ggagcaaata ttgtccgtat tcattcttct
     4981 tcagaggttg cgccaactcg agcacatctt cacgactgat atagcccatg cgataggcaa
     5041 tttcttccaa gtttgcaact tgaacattct gcatacgctg aaccgtttca atatactgag
     5101 aagcttctag caggctttca tgggttcccg tatccaacca ggcaaaacca cgccccatca
     5161 gctcaacaga aaggtcacca cgatttaggt aaaccttgtt gatatctgta atttctaact
     5221 cgccacgtgc acttggttta atacctttag caatttctac aacatcatta tcatagaaat
     5281 acagaccggt cacggcatag ttggagcgag gattctctgg tttttcttct atggaaatgg
     5341 cattcatatc tgtatcaaac tcgaccacac caaaacgctc tggatccttc acttggtagc
     5401 caaaaacagt tgcacctttc tctttctggg ctgccttttg aagcattttg ctcaaaccag
     5461 gtccatgata gatattgtcg cccaaaatca aggcaacact atcgtcaccg ataaattctt
     5521 caccgataag aaaagcctga gcaagtccat ctggactagg ttgttccgca taagaaagct
     5581 tgatcccaaa ttcggaacca tccaagagca agtccttaaa ccggggcaaa tcttgaggag
     5641 ttgagataat caaaatatct ttaattccag ccaacattaa tgtcgacaac ggataataaa
     5701 tcataggttt atcataaacc ggcatcagct gttttgacgc agcccgagta agtgggtaca
     5761 ggcgggtacc tgagccgcct gcaagaataa tacctttcat aatagtgtac ctttcttttt
     5821 tgataagtct tttactttga caaaccacta ggaggaaagt aaattttaat tgtccatact
     5881 aaattgctat taacatttca aggtcatatc cctaagttta taaatcaata acctagagaa
     5941 tcatataatt ccttcccaca tctctttcac agttctaaaa gtataagtag gctttatact
     6001 cccttgttga atatcatcag ctgttgcttc tcctgttaat acacatactg aggatactcc
     6061 tgcattaata cctgtcatga tatcggtgta taaacgatca ccgattacaa ccgtttctga
     6121 caaagagtaa ttcaattttt ttctaactat gttaaccatc gtcggttcag gtttaccaat
     6181 ataaatagga gttttatcaa ctgatttgct aatcatttcg caaatcgaac cacaatctgg
     6241 aagaaaacca aaggaaactg ggcaacgaac atctggattc gtggcaatga atgggacatc
     6301 tctcgttgac aaaacttcac aggtattacg gattttctca cttgttagtt ctgtatcaaa
     6361 accaactaaa acaagatcta tgttatctct cacttgatcg gtaacatcga ttcccgaatc
     6421 ggatagttct tttaacaatg atttcgttcc ctgacaatac acctttgcgt gaggatattt
     6481 ttcctcgata aaaacaatcg ttgcttgagc agaagtaaaa aaattctccc gtttagccac
     6541 aatacctaat tggttaactt tttcaatata gtcaacaact gactttgagg aattgttcgt
     6601 aataaaaata tactcaccac caatactttg aatatattct aataactgaa gtgtcccatc
     6661 aaacaatcta tcttcttcat aaatggtacc atccatatca aataaaaata attttttatt
     6721 ctttaagtca ctgatatcca tcccaaaata atcaacttta tttatcaaat ctgtcctccc
     6781 tatttgtttt aagcaaatag tttttcagct gcagcaagat cttcatgatt gtcaatttcc
     6841 aaccaacgcc catccagcgg acaagctacg aattcaacag cttggagaat cgcattaagt
     6901 gcgacctcgc tccacagttg aagctcattt ttatcttcaa tgaactctct acatttttca
     6961 aagaaacaag ctcctgcatc aggagaaaat ttataaacat caatagaagc tcctaaagcc
     7021 tcctcaggtg aaatctgttt agaaatttct actaaacgac cattcttttc tacgactttc
     7081 atagattcat caatgtaggt tcctaaatca gttacaattg cgttcggagc ttcatgaagt
     7141 aacaaacttt taatgacgga agcatcataa aacacatctg cattcatcat taagaattca
     7201 ctactcccca ttgcatcttt acctagatag gctgagtaca tattatttgt tattttataa
     7261 tcaacattat caataatctt aatttcaggg tatttcccgg aaa
//
LOCUS       DAVLTM010000094         7216 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLTM010000094 DAVLTM010000000
VERSION     DAVLTM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.095"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            67..5391
                     /locus_tag="VYN16_001664"
     CDS             67..5391
                     /locus_tag="VYN16_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1404169.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGTSTYHYLDYS
                     TLTQTERVQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVDYLELDDR
                     TVSPVEQPKVVTDKGEPEVQPALPEAVVTGKGKTEVQPTLPEAVVTEKGEPEVQPVLP
                     EAVVAEKGEPEVQPALPEAVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPV
                     GTTGVDENRNLIEPPVNDIPEYTEPISTVSEVASEREELPSLHTDIRTETIPKTTIEE
                     SDPSKFIGDNSVKQVGEDGERQIVTSYEELHGKKISESVETVTILKEMKPEIIVKGTK
                     ERPKEKTAPVLTLTKVTEDAMNRSANLNYELDNKDNAEISSIVAEIKDGDTVVKRVDL
                     SKEKLTDAVQNLDLFKDYKIATTMIYDRGQGSETSKLDERPLRLELKKVEIKNIASTN
                     LVKVNDDGTETPSDFMNEKPSEEDVKKMYLKITSRDNKVTRLTVDSIEEVTEEGQKLY
                     KITAEAQDLIQHTDPTKVRNKYVYYIEKPHPKEDNVYYNFKDLVDAMNTDKNGTFKLG
                     ADLNATGVPTPKKWYVDGDFRGTLKSVEGKHYTIHNTERPLFQNIIGGTVTKVNLGNV
                     NINMPWADRIAPIADTIKGGAKIEDVKVTGNVLGRNWVSGFIDKIDNQGTLRNVAFIG
                     NVTSVGDGGQFLTGIVGENWKGLVERAYVDANLIGKKAKAAGIAYWTQNSGDNHKVGV
                     EGAVKKGIVKGTIQVESPVEVGGAVGRLSHHGYIGEVVSMMKVKKGEIFYGSSDMNDD
                     PYWVANNVRGNYVVNGVSEGTISYARAKEHHRIKPISQSEADTKIMMLGITAQDFAIN
                     EPVVNRLNRLTRKEDEYKSTQDYKVDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSN
                     LAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGTKEEMDVTKNADSKVKQVREYTIA
                     GQNVVYTPNMVEKDRNQLIQDIKDKLASVQLISPEVRALMDARKKPEENTDERKNGYI
                     KDLYLEESFAETKANLDKLVKSLVENADHQLNSDEAAMKALVKKVDENKAKIMMALTY
                     LNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMI
                     GGTIGKSELHGFLDYNMRLFTNDTDLNDWFIHAAKNVYVSEPQTTNPDFVNKRHRAFD
                     GLNNGVHNRMILPLLTLKNAHMFLISTYNTMAYSSFEKYGKYTEEARNEFKKEIDKVA
                     HAQQTYLDFWSRLALPSVRDQLLKSENRVPTPVWDNQNYSGIKGINRMGYDEKKVPIA
                     PIRELYGPTWKFHNTNWNMGAMASIFPNPNNNDQVYFMGTNMISPFGISAFTHETTHV
                     NDRMLYFGGHRHRQGTDVEAYAQGMLQTPSSIGHQGEYGALGLNMAYHRENDGDQWYN
                     YDPDKLQTREDIDRYMKNYNEALMMLDHVEADAVLPQLNGDNSKWFKKIDREIRRNLG
                     DGLNNLVAPHQWDNVRDLNQEESSKKLSSINDLIDNNFMTKHGNPGNGRYRPEDFRPN
                     SAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYDKGFTSYVSSKYQGEADK
                     QNQGRLGDDFIIKKVSNNQFSNLEDWKKYWYHDVKSRAEKGFTEITIDGQTIHNYNEL
                     KDLFDKAVTEDLKKAGNYSNTENLKSKVFKALLKNTDGFFNPLFKKDI"
     gene            5558..6904
                     /locus_tag="VYN16_001665"
     CDS             5558..6904
                     /locus_tag="VYN16_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1404170.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFFKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLNLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT         2448 a         1224 c         1483 g         2061 t
ORIGIN      
        1 caatttttga gaataaaaaa tagtgtttac tattaggaaa taaaaagtga atgtggagga
       61 ttagaattgt cattattaaa aaaagataaa ttttccattc ggaaaataaa gggtattgtt
      121 ggttcggtat tcttggggag tcttttattt gcaccgtcag ttgttggtac atcgacttat
      181 cattacttag attatagtac tttgacacaa actgaacgtg ttcaactcaa acaaggtaga
      241 cctgatgaat caaaagagtc atatgctttg gtttatgaaa aagacgcatt accaaacaca
      301 ggaagttctc aatctattat gactgcattg ggtttgttag ctatcgggag tcttattgtg
      361 attatcacaa aagataaaaa gaggaaaaaa atcgctacat ttttgattgt aggggcgaca
      421 ggtttagtta cactatcaac tgcttcagca cttaatttga atgctaacat ccatgagtct
      481 ggacgtgatg gtgtgttgca aatttctggt tacagatatg ttgattactt ggagcttgat
      541 gatagaactg tttctccagt tgagcaaccc aaagttgtga ccgacaaagg cgaaccagaa
      601 gttcagccag cgttaccaga agctgttgta actggaaaag ggaaaactga agtacaaccg
      661 acattaccag aagctgttgt gactgaaaaa ggggaaccgg aagtacaacc agtgttacca
      721 gaagctgttg tggctgaaaa aggggaacca gaagtacaac cagccttacc cgaagcagtt
      781 gtaactgaaa aaggcgaacc agaagttcat gagaagcctg actatacgca accaataggt
      841 gcaaaccttg ttgagcctga agttcatgag aaactagcat acactgaacc tgtaggtaca
      901 acaggagtag atgaaaatag aaacttgatt gagccacctg ttaacgatat tccagagtat
      961 actgaaccaa tatctacagt ttcagaagtt gcatcagaaa gagaagagtt gccttcttta
     1021 cataccgata ttcgtacaga gactattcct aaaaccacta tagaagaatc tgatcctagc
     1081 aaatttatag gcgataattc tgttaaacaa gttggtgagg atggcgaacg tcaaattgtt
     1141 actagctatg aagagttaca tggcaaaaaa atcagcgagt cagttgaaac agtaacaatt
     1201 ttgaaagaaa tgaagcctga aattattgta aaaggtacaa aagaaagacc aaaagaaaaa
     1261 acggctcctg ttttaacctt gaccaaagta acagaagatg ctatgaatcg ttctgccaac
     1321 ttaaattatg agcttgataa taaagataat gctgaaatat caagtattgt agctgaaatt
     1381 aaagatggtg atacagtagt taaaagggtt gatttatcta aagaaaaatt aactgacgct
     1441 gtacaaaatt tggatttgtt taaagattat aagattgcaa caacaatgat ttatgatcgt
     1501 ggtcaaggtt ctgaaacgtc taaactggat gaaagacctc tgcgtttgga gttgaaaaag
     1561 gtcgaaatta aaaatatcgc atctactaat ttagtgaagg taaatgatga tggtactgaa
     1621 acacctagtg attttatgaa tgaaaaacct tcagaagaag atgttaaaaa aatgtactta
     1681 aaaatcacta gtcgcgataa taaggtaact cgtttaactg tagactctat tgaagaagtg
     1741 actgaagaag gtcaaaaact ttacaaaata actgctgaag cacaagattt gattcaacac
     1801 acagacccaa cgaaggttcg taataagtat gtttattata tagaaaaacc ccatcctaaa
     1861 gaagataatg tctactataa ctttaaagat ttagtagatg ctatgaatac tgataaaaat
     1921 ggtacattta aacttggtgc agatttgaat gccactggtg ttccaacacc taagaaatgg
     1981 tatgttgacg gcgactttag aggtacattg aagagtgtag aggggaaaca ttatactatc
     2041 cacaacacgg agcgtccgtt attccagaat attattggtg gtacagtaac caaagtaaat
     2101 ctgggaaatg taaatatcaa tatgccatgg gcagaccgca tcgcaccgat tgctgacact
     2161 attaaaggtg gcgctaaaat tgaagatgtc aaagtgactg gtaacgtatt aggtagaaac
     2221 tgggtatctg gatttattga taagattgac aaccaaggta cactcagaaa tgtagccttt
     2281 atcggaaatg tgacatctgt aggtgatggt ggtcaattct tgaccggtat cgttggtgag
     2341 aactggaaag gactagtcga acgtgcgtat gttgacgcaa atcttattgg caagaaagcc
     2401 aaggctgctg gtattgctta ttggacacaa aacagtggtg acaatcataa agttggtgtc
     2461 gaaggtgcag tcaagaaagg aattgtgaag ggtaccattc aggtagaaag tcctgttgaa
     2521 gtcggcggtg ctgttggacg tttgagtcat catggttata tcggggaagt cgtatctatg
     2581 atgaaagtta agaagggtga aattttttat ggttctagtg atatgaatga tgacccatat
     2641 tgggtggcga ataatgtacg aggaaattat gtagttaatg gtgtgagtga aggtacgata
     2701 tcttatgctc gtgctaaaga acaccatcgt atcaaaccaa tttcacagtc agaagcagat
     2761 actaaaatta tgatgttagg tattacagca caagattttg ctatcaacga acctgtggtc
     2821 aatcgcttaa accgtttaac cagaaaagaa gatgaatata agtcaaccca agactataaa
     2881 gtagatcgag atctggctta tcgtaatatt gagaaacttc aaccatttta taacaaagaa
     2941 tggattgtca accaaggtaa caaacttgca gaagattcaa acttagctaa gaaagaagtc
     3001 ctttctgtta cgggtatgaa ggatggacag tttgtaactg acttatcaga tattgataag
     3061 attatggttc actatgctga tggtactaaa gaagaaatgg acgtcactaa gaatgctgat
     3121 tctaaagtga aacaagttcg tgaatatacc atcgctggac aaaacgttgt ttatacacca
     3181 aatatggttg agaaagaccg taaccaattg attcaagata tcaaagataa gttggcgtca
     3241 gttcaattga tttctcctga agtacgtgct ttgatggatg cacgtaagaa accagaagaa
     3301 aataccgatg aacgtaagaa tggttatatt aaagaccttt acttagaaga aagctttgct
     3361 gaaacgaaag ctaatcttga taaattggtt aaatccttag ttgaaaatgc agatcatcaa
     3421 ttaaatagtg acgaagcggc tatgaaagca cttgttaaga aggtggatga aaataaagct
     3481 aaaatcatga tggctctgac ttatctcaat cgttactatg atatcaagta tggggatatg
     3541 actatcaaga acttgatgat gtttaaacct gatttctatg gtaaatcagt tgaccttctt
     3601 gatttcttga ttcgaattgg atcaagtgag agaaatatca aaggtgacag aactttggat
     3661 gcttatcgtg atatgattgg tggaactatt ggtaagtctg aattacatgg attcttagat
     3721 tacaatatgc gtctcttcac taacgatact gatttaaatg attggttcat ccatgcagct
     3781 aagaatgtat atgtatcaga acctcaaaca acgaacccag attttgttaa taaacgacat
     3841 cgtgcatttg atggtttgaa caacggtgtt cataatcgta tgattctacc tctattaact
     3901 cttaagaatg cacatatgtt cttgatttca acttacaata caatggctta tagttctttt
     3961 gagaaatatg gtaagtatac agaagaagct agaaatgaat tcaaaaaaga aattgataag
     4021 gttgcacatg ctcaacaaac atacttagac ttctggtcac gtttagcatt gccaagcgta
     4081 cgtgaccaat tactcaagag tgagaatagg gtaccaactc ctgtgtggga taaccaaaac
     4141 tatagtggta ttaaaggaat aaaccgtatg ggttatgatg agaagaaagt accaattgcg
     4201 ccaattcgtg agttatatgg tccaacttgg aaattccata ataccaactg gaatatggga
     4261 gctatggcat ctattttccc aaatcctaat aacaatgacc aagtttattt catgggaaca
     4321 aatatgatta gtccgtttgg tatttcagcc tttactcatg aaactacaca cgttaatgat
     4381 agaatgcttt acttcggtgg tcatagacac cgtcaaggta cagacgtcga agcctatgct
     4441 caaggtatgc tacaaactcc gtcaagtatt ggtcaccaag gtgaatatgg tgctttaggt
     4501 ttgaacatgg cttatcatcg tgaaaatgat ggtgaccaat ggtataacta tgaccctgat
     4561 aagcttcaaa ctcgtgaaga tatcgatcga tatatgaaaa actataatga agcactgatg
     4621 atgctagatc atgtagaagc agacgccgta cttccacaat tgaatggtga taatagcaag
     4681 tggttcaaga aaattgatag agaaatacgc agaaatcttg gggatggttt gaataactta
     4741 gtagcaccgc accaatggga caacgttcgt gacttaaatc aagaagagag ttctaagaaa
     4801 ttatcaagta ttaacgattt gattgataat aatttcatga ccaaacacgg aaatccaggt
     4861 aacggacgtt atagaccaga ggattttaga ccaaactcag cttacgtaaa tgtcaatatg
     4921 atggcaggaa tttacggtgg gaatacgagt caaggtgctc ctggttcatt atcgttcaaa
     4981 cataatgcct tccgtatgtg gggttattat ggatatgaca aaggtttcac tagttatgta
     5041 tctagcaaat atcaaggtga agctgataaa caaaaccagg gtagattagg tgatgatttc
     5101 attatcaaaa aagtttcaaa caaccaattc tcaaatcttg aagattggaa gaaatattgg
     5161 tatcatgatg tgaaatccag agctgaaaaa ggattcacag agattacaat tgatggacaa
     5221 accattcaca actataatga actgaaagac ttattcgaca aagctgtcac agaagacctc
     5281 aagaaagctg gaaactactc aaatactgag aatttgaaat ccaaagtctt caaagctctt
     5341 cttaaaaaca cagacggttt ctttaatcca ttgttcaaaa aagacattta aaccccaatt
     5401 atgcacccct aatctaaaaa ccatccagaa tccttgcctt agcttagatc ctggatggtt
     5461 tcttttttca cccaatgggt gttttttact agacaaaaaa gagtttcccc tttatggtat
     5521 aagtgtagaa aaaaacacaa aaagaaagga aactcatatg aacagtttac caaatcatca
     5581 cttccaaaac aagtcttttt accaactatc tttcgatgga ggtcatttaa cccagtatgg
     5641 tggtcttatc ttttttcagg aacttttttc ccagttgaaa ctaaaagagc ggatttctaa
     5701 gtatttagta acgaatgacc aacgccgcta ctgtcgttat tcggattcag atatccttgt
     5761 ccagttcctc tttcaactgt taacaggcta tggaacggac tatgcttgta aagaattgtc
     5821 agctgatgcc tactttccaa aattgttgga aggagggcag cttgcttcac agccaacctt
     5881 atcccgtttt ctttccagaa ctgacgagga aacagtccat agtttgcgat gcctcaacct
     5941 tgaattggtc gaattctttt tacagtttca ccagctaaac caactcattg tagatatcga
     6001 ttctacccat ttcacaactt atggcaagca agaaggtgtt gcttataacg cccactatcg
     6061 tgctcatggc tatcatcctc tttatgcttt cgaggggaag acaggttatt gtttcaatgc
     6121 ccagcttcgt cctggtaatc gttattgttc tgaagaggca gacagcttta tcacacctgt
     6181 tttagaacgg tttaatcaac ttctctttcg aatggatagt ggctttgcga ccccaaaatt
     6241 atacgattta attgaaaaaa cagggcaata ctacctcata aaactcaaga aaaatactgt
     6301 tctgagccgt cttggagacc tttccctccc ttgcccacag gatgaggact taaccatctt
     6361 gccccactcc gcctactcag aaactctcta tcaagcagga tcttggtcgc acaagcgtcg
     6421 tgtctgccag ttctctgaac gaaaagaagg aaacttgttc tacgatgtta tttctctcgt
     6481 tacaaatatg acgagtggaa caagccaaga ccagtttcag ctttatcgtg gacgtggtca
     6541 agccgagaat ttcatcaagg agatgaagga gggatttttt ggcgataaaa cggatagttc
     6601 aaccttaatc aaaaacgaag ttcgtatgat gatgagctgt atcgcctaca atctctatct
     6661 ttttttcaaa catctagctg gaggtgactt ccaaacttta acaatcaaac gcttccgcca
     6721 tctttttctt cacgtggtag gaaaatgtgt tcgaacagga cgcaagcagc tcctcaactt
     6781 gtctagtctc tatgcctatt ccgaattgtt ttcagcactt tattctagga ttagaaaagt
     6841 caacctgaat cttcctgttc cttatgaacc acctagaaga aaagcgtcgt taatgatgca
     6901 ttaaagaaca gtcgagatga aaaaatcgtg tgacgcacca agggaggagt ctgccctttt
     6961 gaggaaatct agcgaggaaa aacgatactg gaacagcaga aagtaaaact gacctcatga
     7021 ggaggaagaa agtggctcat gaggtcaggg gttttgtaag ttacatctag ttgagagagg
     7081 tatgaatgat ttggggtttc aataacctaa ctaagtttaa ttcagttaga tttacttgat
     7141 tttcttggat gctataatca ggatttttat gtctagcttt tttatagtaa aaatcgaaat
     7201 ctttttctgt aacttc
//
LOCUS       DAVLTM010000095         7036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLTM010000095 DAVLTM010000000
VERSION     DAVLTM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.096"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            12..719
                     /locus_tag="VYN16_001666"
     CDS             12..719
                     /locus_tag="VYN16_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1404171.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            859..1035
                     /locus_tag="VYN16_001667"
     CDS             859..1035
                     /locus_tag="VYN16_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1404172.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            1165..2100
                     /locus_tag="VYN16_001668"
     CDS             1165..2100
                     /locus_tag="VYN16_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1404173.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            2093..2881
                     /locus_tag="VYN16_001669"
     CDS             2093..2881
                     /locus_tag="VYN16_001669"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1404174.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGVLVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            2882..3265
                     /locus_tag="VYN16_001670"
     CDS             2882..3265
                     /locus_tag="VYN16_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1404175.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            3281..3670
                     /locus_tag="VYN16_001671"
     CDS             3281..3670
                     /locus_tag="VYN16_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1404176.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            3754..4884
                     /locus_tag="VYN16_001672"
     CDS             3754..4884
                     /locus_tag="VYN16_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1404177.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            4889..5626
                     /locus_tag="VYN16_001673"
     CDS             4889..5626
                     /locus_tag="VYN16_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1404178.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSVVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            5637..6410
                     /locus_tag="VYN16_001674"
     CDS             5637..6410
                     /locus_tag="VYN16_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1404179.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            6400..7017
                     /locus_tag="VYN16_001675"
     CDS             6400..7017
                     /locus_tag="VYN16_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1404180.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
BASE COUNT         2006 a         1299 c         1618 g         2113 t
ORIGIN      
        1 aggatgaaaa gatgaaagtt attaaagttg aaaaccaagt tcaaggtgga aaagttgctt
       61 ttgagatttt gaaggaaaaa ttggctaatg gagctcaaac tctaggactt gcgacaggaa
      121 gtagcccact tgaattttac aaggaaattg ttgagagtaa ccttgatttt tcaaatctaa
      181 ccagtgtcaa ccttgatgag tatgtgggcc ttgatgggga caatccacag tcttatcgtt
      241 acttcatgca agaaaacttg ttcaaccaaa aaccatttaa agaaagtttc ttgcctcgtg
      301 gtgttaagga caatgctgaa gctgaagttg aacgctacaa ccaaattttg gctgaccatc
      361 cagttgacct ccaaatcttg ggaatcggtc gcaatgggca tatcggcttt aatgagcctg
      421 gtactccatt tgacagtcaa acgcatctag tagaacttga ccagtctact attgaagcca
      481 atgcacgctt ctttgccaag attgaagacg tcccaaccca agctatttca atggggatta
      541 aaaacatttt ggatgccaag tctattattc tttttgctta cggtgagtcg aaagcagaag
      601 ccattgccgg aacagtatct ggcccagtga ctgagaacct acctgcaagt agcctccaaa
      661 atcaccctga tgtaaccatc atcgcagatg ctgaagcgct tagcttactc gaaaaataaa
      721 aaagcaatgt tctattgaac gctttcaact cttgtataaa tggaagaaaa aatcaaaatt
      781 aaaccgcatt tttgcttgac aattattcct tttacgtgta gaatgaaata gatattgaac
      841 ttgaagggag tgaaaaaaat gtctaaaaca gtagtacgta agaatgaatc tcttgacgac
      901 gcacttcgcc gtttcaaacg tgcggttact aaagctggta ctcttcaaga aacacgcaaa
      961 cgtgaattct atgaaaaacc ttctgtaaaa cgtaaacgta aatcagaagt agctcgtaaa
     1021 cgtaaaaaat tctaattgca atgaaaggct agagaaatct agtccttttt cttttaaata
     1081 aatactccaa agcctgcaaa aatctgaaac ttcctcctac aatttgatat aatagagaga
     1141 agaactcatt tgaaggagga aatgatgtcg gttttagtaa aagaagtgat tgaaaagctt
     1201 agactagata ttgtctatgg tgaaccagag ttgcttgaaa aggaaatcaa tacagcggat
     1261 attacgcgac ctggtcttga aatgacaggc tattttgact actatacacc agagcggatt
     1321 caacttttgg ggatgaagga gtggtcttat ctgatcagca tgccttccaa cagccgttat
     1381 gaagttttga aaaaaatgtt tctacctgag acaccagcag tcattgttgc ccgtggtttg
     1441 gtggttccag aggagatgtt aaaggctgct agagaatgta agattgctat tttaaccagc
     1501 cgtgcagcta ccagtcgttt atctggagag ttatctagct atctggattc tcgtttggca
     1561 gaacgtacca gtgtgcacgg tgtcttgatg gatatttatg ggatgggcgt cttgattcag
     1621 ggagatagtg gaattggtaa gagcgagaca ggtcttgagc ttgtcaaacg tggtcaccgt
     1681 ttggtagccg atgaccgtgt cgatatcttt gccaaggatg agattactct ctggggtgaa
     1741 ccagctgaaa ttttgaaaca cttgattgaa attcgtgggg ttggtattat cgatgttatg
     1801 agtctctacg gtgcgagtgc tgtcaaggat tcttcacagg ttcagcttgc tgtctatttg
     1861 gaaaattacg atacgcataa gacctttgat cgtcttggaa acaatgcaga ggaacttgaa
     1921 gtttctggcg tagccattcc tcgtattcgt attccagtta aaacaggtcg taatatctct
     1981 gttgtgattg aggcagctgc catgaattat cgtgccaagg aaatgggctt tgatgcgacc
     2041 cgtttgttcg acgaacgact gacaagtcta atcgctcgaa atgaggtgca aaatgcttga
     2101 tccaattgct attcaactag gacccctagc cattcgttgg tatgccttat gtattgtgac
     2161 aggcttgatt cttgcggttt atttgactat gaaagaagca cctagaaaga agatcatacc
     2221 agacgatatt ttagatttta tcttagtagc ctttcccttg gctattttag gagctcgtct
     2281 ctactatgtt attttccgat ttgattacta tagtcagaat ttaggagaga tttttgccat
     2341 ttggaatggt ggtttggcca tttacggtgg tttgataact ggggttcttg tgctctatat
     2401 ctttgctgac cgtaaactca tcaatacttg ggattttcta gatattgcgg cgcctagcgt
     2461 tatgattgct caaagtttgg ggcgttgggg taatttcttt aaccaagaag cttatggtgc
     2521 aacagtggat aatctggatt atctacctgg ctttatccgt gaccagatgt atattgaggg
     2581 gagctaccgt caaccgactt tcctttatga gtctctatgg aatctgcttg gctttgcctt
     2641 gattctgatt tttagacgga aatggaagag tctcagacga ggtcatatca cggcctttta
     2701 cttgatttgg tatggtttcg gtcgtatggt tatcgaaggt atgcgaacag atagtctcat
     2761 gttcttcggc cttcgagtgt cccaatggct gtcagttgtc cttatcggtc tcggtataat
     2821 gatcgttatt tatcaaaatc gaaagaaggc cccttactat attacagagg aggaaaacta
     2881 aatgttagaa gttgcatata ttcttgttgc cctagctttg attgtctttt tggtctatct
     2941 gatcattact gtacaaaagc ttggtcgtgt catcgatgaa acagaaaaga cgattaaaac
     3001 cttgacttca gatgtggatg tgaccttgca tcacaccaat gagttgttgg ctaaggtcaa
     3061 tgtcttggca gatgatatca atgtcaaggt ggctacaatt gatccactct tcagtgctgt
     3121 tgcagattta tctctatctg tttcagacct caatgaccat gcgcgtgtct tgagcaagaa
     3181 agcttcatca gctggttcaa aaacactcaa gactggtgca agtctgtcag ctcttcgtct
     3241 tgcaagtaaa tttttcaaaa aataaaaaag gagaatcctt atgggtaaat tatcctcaat
     3301 ccttttagga acggtttcag gtgcagctct tgccttgttt ttaacaagtg ataagggcaa
     3361 acaagtttgc agtcaggctc aagattttct agatgatttg agagaagatc cggagtatgc
     3421 caaggagcaa gtctgtgaaa aactgacaga agttaaggag caggctacag attttgttct
     3481 gaaaacaaaa gaacaggttg agtcaggtga aatcactgtg gacagtatac ttgctcaagc
     3541 taaatcctat gcttttcaag cgacagaagc atcaaaaaat caattaaata atctcaagga
     3601 acaatggcaa gaaaaagccg aagctcttga tgactcagaa gagattgtga ttgatataac
     3661 agaagaataa accatcacca tctccggacg gactatgtat ctggggatgg tgatttttat
     3721 ctggaatcta gtctttgtgg tataataatt actatgcaga aaaaaccaac gtcagcctat
     3781 gtgcacatcc cattttgtac ccagatttgt tattattgtg atttttcaaa ggtcttcatc
     3841 aaaaatcagc cagtcgacag ctatttagag catctgctgg aagagtttcg ttcttatgat
     3901 attgaaaagt tgtcaaccct ttatatcggt ggtggaacac cgacagccct gtcggctccg
     3961 caactggagg tgttactgaa tggcttgact aaaaacttgg acttgtctgt cttggaagag
     4021 ttgaccattg aagccaatcc aggcgatttg gatgcggata agatagctgt tttgaaaaat
     4081 tcggctgtca atcgtgtttc gctaggtgtc cagacctttg atgataagat gttgaaaaag
     4141 attgggcgca gtcatttgga gaaggatatt tatgaaaata tcgatcgtct gaaactggct
     4201 ggttttgaca acatctccat tgatttgatt tatgctctgc ctggtcagac catggagcaa
     4261 gtaaaggaaa atgtggctaa agccattgga ctggatattc cccacatgag tttgtatagt
     4321 ttgattttag aaaaccatac ggtctttatg aaccggatgc gacgtgggaa attgcctctg
     4381 cctaaggagg aactagaagc ggagatgttt gagtacatca ttgcagagct ggagcgagcg
     4441 ggttttgagc attatgagat ttctaatttc tccaaacccg gctttgaaag tcgtcataat
     4501 ctcatgtact gggacaatgc tgaatactat ggtattggtg caggggcatc tggttatgtc
     4561 aatggagtac gttataaaaa tcatggtccc attcgtcatt atctcagtgc ggttgaggaa
     4621 ggcaatgctt gtattacaga agatcacctg agtcaaaagg agcaaatgga agaagaaatg
     4681 ttcttgggac tccgcaagaa atccggggtt tccatggcgc gatttgagga aaaatttgga
     4741 cagtcttttg ctggacttta tggagaaatt gtcagagatt tggttcaaca aggcctcatg
     4801 cagattgagg gtgaccacgt tcgcatgaca aagagaggtc tctttttggg agatactgta
     4861 gcagaacgat ttattttgga gtaggatcat gggcttaact tatcaaatga aaatgaaaat
     4921 tccttttgat atggctgata tgaacggtca tatcaaactt ccagatgtga ttttgctgtc
     4981 actgcaagtt tcagggatgc agtcgattga actgggagtt agtgataagg ccattttgga
     5041 agactataat ctggtctgga ttatcgcaga atatgatatt gaggtggttc gtttgcctcg
     5101 ttttgcggaa gaaattacca tcgaaacgga agccttgagc tacaatcgac ttttttgcta
     5161 ccgtcgcttt actatttata atgaagcggg gcaggagctc atccatatga tggcgacctt
     5221 tgttctcatg gaccgcgaca gtcgaaaagt ccatgctgtt gaacctgaga ttgtggctcc
     5281 ttaccagtct gattttgata aaaaacttat ccgtggaccg aagtatgagt ccttggaaga
     5341 accgatcagc aaggattacc atgttcgttt ttacgacttg gatatgaatg gtcatgtcaa
     5401 taacagtaaa tacttggact ggatttttga ggtcatggga gcggattttt tgacccacta
     5461 tattcccaag aaaatcaacc tcaagtatgt caaggaagtt cgaccaggtg gagtgattac
     5521 atcggttgtt gaacggactg gactggaaag caagcatgaa attacaagtg atggggttat
     5581 caatgcccaa gcaatcatta cttggcaaga aataaaaaag gcttagggag aaatagatga
     5641 aatataaagg ctatttaatt gatttagacg gaaccattta taaggggaaa gaccgaatcc
     5701 ctgctggaga gacttttgtt catgaattgc aaaagcgggg cattccctat ctctttgtaa
     5761 ccaacaatac aactcgcact ccagagagtg ttaaggagat gttggctcag aattttaata
     5821 tcgatacgcc cctatcgact gtctacacag cgactttggc aaccatcgac tatatgaatg
     5881 atttggggct tgaaaagacc gtctatgtcg tcggagaagc aggactcaag gaagccatca
     5941 aggcggctgg ttatgtggaa gacaaggaaa accctgccta cgtggtagtt ggtctggatt
     6001 ggcaagtcga ctatgaaaaa tttgccacag caactcttgc tattcaaaag ggtgcccatt
     6061 ttatcggaac caatcctgac ctcaacatcc cgacggaacg cggtcttttg ccaggtgctg
     6121 gctcactgat tacgctgctt gaagtagcga cacgagtgaa gccggtttat atcggaaaac
     6181 caaatgccat cattatggac aaggcggttg agcacttagg tttggaacgt gaagagttaa
     6241 tcatggttgg ggacaattat ttgactgata tccgggctgg gattgacaat ggcattccaa
     6301 cgctcttggt gacgacaggt tttactaagg cagaagaagt agctggcctg ccaatcgcac
     6361 cgacacatgt ggtttctagc cttgcggagt gggattttga tgaaaactaa attgaccttt
     6421 tggggctcta tgctctttct cctctccctc tcaatccttc tgaccattta tctggcttgg
     6481 attttctatc ctatggagat tcagtggcta aacttaacga atcgagtcta tctaaaacca
     6541 gaaaccattc aatacaattt tcatatcttg atgaattatc tgaccaatcc ttttagtcag
     6601 gtcttacaga tgcctgattt tcgttcgtca gcagctggtc tgcaccattt cgcagtggtc
     6661 aagaatctct ttcatttggt tcagctagta gctctagtga cactgccaag tttctatgtc
     6721 tttgtcaata ggattgtgaa aaaggacttt ttgtctcttt atcgaaaaag tctcctggct
     6781 ctagtagtct tacctgtgat gattggactt gggggagttt tgattggttt tgaccaattc
     6841 tttactcttt tccatcaaat tctctttgtg ggagatgata cctggctttt tgatccagcc
     6901 aaggatcctg ttattatgat tttgccagag accttctttc ttcatgcctt cctcctcttt
     6961 tttgccctct atgaaaactt ctttggctat ctgtatctga aaagtcgtag gaagtgaaag
     7021 gaaagatgtt tttaat
//
LOCUS       DAVLTM010000096         6788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLTM010000096 DAVLTM010000000
VERSION     DAVLTM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.097"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(4..1554)
                     /locus_tag="VYN16_001676"
     CDS             complement(4..1554)
                     /locus_tag="VYN16_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1404181.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1570..2349)
                     /locus_tag="VYN16_001677"
     CDS             complement(1570..2349)
                     /locus_tag="VYN16_001677"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1404182.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAIQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2336..3187)
                     /gene="ylqF"
                     /locus_tag="VYN16_001678"
     CDS             complement(2336..3187)
                     /gene="ylqF"
                     /locus_tag="VYN16_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1404183.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEPSVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(3268..4776)
                     /locus_tag="VYN16_001679"
     CDS             complement(3268..4776)
                     /locus_tag="VYN16_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1404184.1"
                     /translation="MYYVTKTNSKGQPLYQVVEKYKDPLTDKWRTVTVSYTRNTSRAR
                     KQAEREVMDKIEKLTSSLESQYNPEMITTFGELKESWFQTWCVSVKPQTIKREELVIE
                     RLGKIIGDEFLLDRITPLLMKNCLNKYLDKYDASPSTMTHIKSTCNKIFNHGVLYNVI
                     KYSPMTAVKIDVPLEKKREARKRLDAKFLEIHELQAFFDVLGQCRNASYYDLAIVLLL
                     TGIRIGEAAFLPSDVDFDKGILHIDKALQYHDLKVDEFYFDTTKTVNADREVALPIAA
                     SEAIQRAIKRNQEFEAYMEQNPSPAFKKCDSVFQTEYGSPITSHSFRQVLKRVEEKLL
                     KNCQKDYGFKWVKHVTPHSFRHMHISYLQSNEMHIAVKDIMTRVGHANFETTMGYTHN
                     ISRSQENTVTALNQFVEKHNFHFKELKSYTCKYSRMIEQFIETCDDNNKVELSVDEFK
                     DLLHLGSRYSPKHIVSNLLLKIKKDIVKYHPQFDIKIVKLSGNQIKGFSISW"
     gene            complement(4852..5085)
                     /locus_tag="VYN16_001680"
     CDS             complement(4852..5085)
                     /locus_tag="VYN16_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1404185.1"
                     /translation="MEIKYVSVENSVAEWADKKSILKRWEGLNLYTLNRWIKEMRENK
                     RFSLYVINPTHKLVFINLEGFECFLRWKQKRTK"
     gene            complement(5387..5610)
                     /locus_tag="VYN16_001681"
                     /pseudo
     CDS             complement(5387..5610)
                     /locus_tag="VYN16_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5867..6340)
                     /gene="tnpA"
                     /locus_tag="VYN16_001682"
     CDS             complement(5867..6340)
                     /gene="tnpA"
                     /locus_tag="VYN16_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEV1404186.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVLSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            complement(6498..>6788)
                     /locus_tag="VYN16_001683"
     CDS             complement(6498..>6788)
                     /locus_tag="VYN16_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404187.1"
                     /translation="LTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQ
                     LNPLTYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
BASE COUNT         2018 a         1391 c         1212 g         2167 t
ORIGIN      
        1 gtactacaac aacaaatctt tgacttttcc aatttcactt tttggaataa ccaggtgaat
       61 catatcaccc agatacattc tggttgagcc gttaactgtt tggctcttgc cattatggac
      121 ttgagttgtg atgaggacgt tgtgtggtaa gttgagttca tgaacttgtt tcccagcaat
      181 tttatcagaa actggtattt cgataagtgt aacttctcct tcgctagata cttcttctgg
      241 aagcattttt tccagcatgg cttcatagac tggcgtacct ttgagcaaat ccatgataat
      301 ataagaaaca agagtgacaa gaccaagtgg catgaggttg cgaatatctc ctaccatctc
      361 agttacgagg atcatagcgg ttaagggagc ttttgatatg gctccaaaat agccactcat
      421 tcctagaatg acaaatatag ggaattgctc ttgactgaca agtccaagat tgacacagat
      481 aacaccaatt aaggcaccaa gcaaagaacc aagagctaaa atggggagga aaattcctcc
      541 tggcagtcca cttccatagc taatcatact ccaaataaag cggatcaaaa agtaagctaa
      601 taaaacttgg aaactaaaat tttgttcagt taaagaaagg acaagctgat ttccgccacc
      661 aatgatttga ggtaagaaga ttccgactgg tatgataagg ataaaagcca agatgggata
      721 ataagctcta tccaaatgga ttttttgacc aatcaagtca taaactcttc caacgtttaa
      781 tacagctttc tcatagagaa agcctgaaaa tccaaggaaa attcccatga cgagatatat
      841 ccaatactga tctagggtca tgggaggaat gttatctggc atatccaata ctggtgtcaa
      901 accgaacatg agtagagaca caaagtttgc tacgatgctg gctgctagag ttgagaccca
      961 gaaaaagcgc gaaaaatggt gatagacttc ttctacaaca aagagaagtg ctgcaatagg
     1021 agcattaaag gctgcggcta aacctgctgc agctccactg gcaatcaagg aacgttcctc
     1081 tactggactg gatttgagcc acttggcaat tcctttacca ccaactgctc caagttgaat
     1141 actgggtccc tctcgaccca gcatgagtcc actggcaata gcgagaatac ctagcacata
     1201 ttttttccaa agaatgcccc accagttgag ggacatgagg cctttcagtt cggcttcgac
     1261 ttgaggaatt cctgagcctt taatatcttt ttctgaccgt gttagtttgg cactgagcca
     1321 acagatgagt atataaaaca aaaccagtac aaaaagattg cgcactaagt acccttgatc
     1381 ttgataaact ccttgtatca tgtggaagcc cttttcaatt aagaaacgaa aggatccgac
     1441 gataattcca acgatgaggc cgacaatgat tcctcgacca acttgggata gtatagtgct
     1501 ggatgcaaag gcgaattctt tcttagaacg gactatttcc gactgttcct ccatggtatt
     1561 tcctttcaat taactttctt ttttacctaa aaccagtgat ttaacgggtt caaagctggt
     1621 tcggtgaatt ggggtaactc ctagttttgt gaggccttcc agatgtttag ctgtgccata
     1681 tcctgcatta gtagcgaaat catagccagg gaactgctga tcgtattctt tcatcaattc
     1741 atcacgtgtt accttggcta ctatagatgc tgctgcgata gagagggagt tggcatctcc
     1801 tttgataatg gaggtttgtg aaatgggcaa gtccagtttc atggcatcaa tcaaaaggtg
     1861 ctctggttga gggctgagct gggagattgc ttcttgcatg gctagtttgg ttgcttcata
     1921 gatgttgact tggtcgatga cctgattatc tatgatacca attccaatcg acaaggcttg
     1981 gtcttgaatg gcttggaaaa tctccagatg tttcttttta ggaattttct tgctgtcgtt
     2041 gagaccttta atcttacaat ttttaggtaa aataacggca gcagcgacta caggaccagc
     2101 aagaggacca cgaccaacct catcaatacc tgcaattaag gtcaatcctt gcttataaag
     2161 ttctttttca taagaaagca tggattccaa gcgcaaattt tcatctaatt cagcttggat
     2221 ggctctttta cgcttgctga tttccttttg aactcctgag cgattatcct tttcaagctc
     2281 taaaaaaata gggctttcta actctttgac tgtcacaagg agttctttga tttctttaat
     2341 cgttgccatc gaggtcttcc aatgtatcta aggtatagtt accgagtttg ccatcacgga
     2401 cttccttcac gaagagactg taaaaacggt catagtcatc acggaaaccg agggcgcggg
     2461 tcatatccat aataatcaca gacggttctt cttcaatttt catttgtttg aagcgttcag
     2521 ccagcttttc tggataatgt tctttgaaat aattgatacc aaaaatggta acctcatcca
     2581 taggaagcaa ctggtctttg atagctccag tcaatgccaa cttaagtgca acagtttcat
     2641 cctcaaactt aggccagaga atccccggtg tatccaagat ttccaggtct ttattggttt
     2701 taagccattg ttgacctttt gtgacccctg gcttgtttcc aacaacagca atctttttac
     2761 cagccaaacg gttcatcaga gttgatttac cagcgtttgg aatcccgata atcatggtac
     2821 gcaaggtttc aatctgaatc ccacgttctt tctggcgagc aatcttatca gccatgagct
     2881 tcttggccgc atctgttaca acttttacag tcacttgctc tttggagttg atagctagcg
     2941 tctggattcc ttgtgattca aaatactgac gccattcctt ggtcattgct ggatcagcca
     3001 agtcggcctt gtttaaaatc aagagttttg gtttatcacc aacaatcttg gtcaacatag
     3061 gattttgact agatagaggc aagcgtgcat ctactaaaat cgtcacaaaa tcaacaaatt
     3121 ttaaattctc ctgcacctgt cgacgagctt tagacatgtg accaggaaac cattgaatag
     3181 tagccattga gttttttcct ttcgtagcaa gggtttagca cccctcattt attttactat
     3241 cgtctaaaca ccaagcgaac accaaaacta ccacgaaatg gaaaaacctt tgatttgatt
     3301 cccgcttaat ttcacaatct ttatatcaaa ctgtgggtga tatttgacaa tatctttttt
     3361 gatttttagc agtaaatttg aaacaatatg tttgggtgag taacgtgaac caaggtgtaa
     3421 taagtcttta aattcatcaa cacttaactc tactttattg ttatcatcac aagtttcaat
     3481 gaattgttca atcattctcg aatatttaca ggtgtaactc ttcaattctt taaagtgaaa
     3541 attgtgcttt tctacaaatt gatttaaagc agttacagta ttttcttgtg aacgactgat
     3601 gttatgggtg tagcccatcg tggtttcaaa attcgcatga ccaactctag tcataatatc
     3661 ctttacagct atgtgcattt cattactttg aagataacta atgtgcatat gtctgaaaga
     3721 atgaggggtc acatgcttga cccatttaaa accataatcc ttctgacaat tttttaacaa
     3781 cttctcctca accctcttca aaacttgacg aaatgagtga gaagttatcg gagaaccata
     3841 ctcagtttga aatacactat cacacttttt gaaagcagga ctaggatttt gttccatata
     3901 ggcttcaaat tcctgatttc tttttatagc tctctggatt gcttcactcg ctgctattgg
     3961 gagggctacc tctctatcag cattgacggt cttagtcgta tcaaaataaa attcatcaac
     4021 ttttaaatca tgatattgga gagccttatc aatatgtaaa atccccttat caaaatcaac
     4081 atctgatggt aaaaaagcag cctctccaat ccgaatccct gtcagtaata atacaatagc
     4141 aaggtcataa tagctagcat ttctacactg ccctaaaaca tcaaaaaagg cttgcaattc
     4201 atgaatttca agaaatttag catcaagcct ttttcttgct tctctctttt tctccagagg
     4261 cacatctatt ttaacagcgg tcattggaga gtacttaatg acattgtata gcacaccgtg
     4321 attaaatatc ttgttacacg tactttttat atgtgtcatt gttgaaggtg aagcgtcgta
     4381 tttatccaga tatttattaa gacaattttt catcagtagt ggtgtaattc gatcgagtaa
     4441 aaattcatca ccgataattt taccaagccg ttctatcacc aactcttccc tttttattgt
     4501 ctggggcttg acagatacgc accaagtttg aaaccaactc tcttttaact ctccaaaagt
     4561 tgtaatcatt tcaggattgt actgactttc aagggaagag gtcagttttt ctattttatc
     4621 cattacctct ctttcagctt gctttcgtgc tctactagta tttctagtat agctgacagt
     4681 tactgttctc cacttatcag taagtggatc tttatatttt tcaaccactt gatacaaggg
     4741 ttgtcctttt gaatttgttt tagttacata atacataatt taattcctcg aaaagaaata
     4801 aatcattgtt gtatagaata aataactact ctatattata ccatgacttc cttattttgt
     4861 ccgcttttgt ttccatctca aaaagcattc gaatccctca agatttataa acaccagctt
     4921 atgtgtagga ttgatgacgt ataaagaaaa tctcttattt tctctcattt ctttgatcca
     4981 tctgttaaga gtgtagagat tcaaaccttc ccatcgtttc agtatgcttt tcttatccgc
     5041 ccactcagct accgaatttt caacagatac gtattttatc tccatattat catcctttcg
     5101 aataatttct tctccctcca tggcatgact gatggggtca tcataagaat ctcacttact
     5161 gctcttttac gacggcagcc tgaacgttca gaagtatcat tgtatatcat ttgcatcatg
     5221 gcagatagag tatcgctcta ttttattggt gattgtcccc gctcctatct tggcgaatca
     5281 agcaaaactg ctccacaaat gaggaaagta ccattttagg ctattcagtt gtcaaagggc
     5341 aaataaaaaa tctcactcat tttcaaactc tatgaaattg aggagattat tttctaaagc
     5401 gaattttatc aggaaaatca cagttaatcc caataaatag tctgtactga cgtcatagaa
     5461 atctgagagt ttaaccaata catcagccgt caacgcatgc tcaccccgtt caattttggc
     5521 ataagctgaa tttgtaaacg aaagtattgt agctatttgc ttttggggca gatcatgatc
     5581 ctccctcaaa tctctcaaac gtctgtacat ctagctttct cctaagaaaa agtatagacc
     5641 atatattgta actatttagg atattgtcca aatcggacaa caggaatttt accatccttt
     5701 atatagactt ttatttttat gtatcaagtt ttttaaatca aaaccaccag tgtgaactgg
     5761 tggtttgttc tgcggctata agccgcttct accggccagg gccaaaggcc catcgaaata
     5821 gcttcctcgc gcaccacttt cccgagcagg tgctaaagca ccttagttac ttcctcttat
     5881 ttatttcgcc agtaaacgga tctactgact cgaataacgt gagctggtct gctactctgt
     5941 cttcttgtaa ttgattctga atatattcag ctatcacttt ctgattacgg cctaccgtat
     6001 ctacataata gcctctacac caaaacttgc gattgccata tttgtatttt aaattcgcat
     6061 gcttatcaaa aatcatcaaa ctgctcttgc cctttaaata gcccataaag gacaaaacac
     6121 taagtttcgg aggaatactg ataagcatgt gaatatggtc tgaacaagca ttcgcttcat
     6181 ggattattac acccttacgc tcacataagt cacgtatgat tcttccgata ctagctttgt
     6241 atctgccata aatgatttga cgacgatatt tgggtgcaaa aacaatatga tatttacaat
     6301 tccatgtggt atgtgataaa ctttgattat cctctctcat gaggtacctc ctgtatgata
     6361 tgttgtagtg gcggagaaac cacttctatc ttatcatttt aggaggttct ttttgttacc
     6421 acgctaaaag ctctatggaa ccactagcat agctagtggt tttcgggaga caacaaaaaa
     6481 gttggatgac ccaactttta tctttctatc agtatcaatt ttatctttgg tataataaaa
     6541 gatacagata taaggacaag taatgatgtt aacaataaga aaactaattc caaagttgtt
     6601 gaatttttaa aataaaagtt tctcaaaata gttaattgat aagttaaggg atttaactga
     6661 ccaatccatc tcacaatact aggagcagaa tctattaagt agaaaacagg agctgtaaaa
     6721 ccaagcggtg ttagtacaaa acgtattaca atatcacgtc ttctgtaatc attgataaac
     6781 attgtaag
//
LOCUS       DAVLTM010000097         6727 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLTM010000097 DAVLTM010000000
VERSION     DAVLTM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.098"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            133..1362
                     /gene="arcA"
                     /locus_tag="VYN16_001684"
     CDS             133..1362
                     /gene="arcA"
                     /locus_tag="VYN16_001684"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1404188.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1423..2436
                     /gene="argF"
                     /locus_tag="VYN16_001685"
     CDS             1423..2436
                     /gene="argF"
                     /locus_tag="VYN16_001685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1404189.1"
                     /translation="MNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYL
                     AGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDA
                     DEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAF
                     HDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYI
                     PKV"
     gene            2601..3548
                     /gene="arcC"
                     /locus_tag="VYN16_001686"
     CDS             2601..3548
                     /gene="arcC"
                     /locus_tag="VYN16_001686"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1404190.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3759..5270
                     /locus_tag="VYN16_001687"
     CDS             3759..5270
                     /locus_tag="VYN16_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1404191.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     HPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5292..6623
                     /locus_tag="VYN16_001688"
     CDS             5292..6623
                     /locus_tag="VYN16_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1404192.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKETGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         1996 a         1362 c         1420 g         1949 t
ORIGIN      
        1 tgctaaacat cataatttat attattttcc gagaaattat agccattgac attacctagg
       61 aattgcgtta taacatacat gtaagcggta cccaaaatgg tgccaagtca aaatttttaa
      121 ggaggaaaat acatgtcttc tcatccaatt caggtcttct cagaaattgg gaaactgaaa
      181 aaagttatgt tgcaccgtcc aggcaaggag ttagaaaact tgttgccgga ctatcttgaa
      241 aggcttcttt ttgatgatat tcctttcttg gaagatgctc aaaaagaaca tgatgcattt
      301 gcccaagctc ttcgcgatga aggaattgag gttctctacc tagaacaact cgctgctgaa
      361 tcattgacct ctccagaaat ccgcgatcaa tttatcgagg aatacttaga cgaagccaac
      421 atccgtgatc gtcaaaccaa ggttgctatt cgtgaattac ttcacggcat caaggacaac
      481 caagaattgg ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc agaaattcct
      541 gacgaagcta aagatctaac tgacttagtt gaatcagagt atccatttgc aattgacccg
      601 atgccaaacc tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc cgtatcgctt
      661 aaccacatgt ttgcagacac tcgtaaccgt gaaacactct acggtaagta tatcttcaaa
      721 taccacccaa tctatggcgg aaaagtggat ttggtctaca accgtgaaga agatacgcgt
      781 atcgaaggtg gagacgagct agttctttct aaagacgtcc ttgcagtagg tatctctcaa
      841 cgtacagacg cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa aaatgttggc
      901 ttcaagaaag ttttggcctt tgaatttgct aacaaccgta aattcatgca cttggatact
      961 gtcttcacta tggtagacta tgacaagttc actattcacc cagaaatcga aggcgacctt
     1021 cacgtttact cagttactta cgaaaacgaa aaacttaaaa ttgttgaaga gaaaggtgac
     1081 ttagctgaac ttcttgctca aaaccttggt gtagaaaaag ttcatttgat tcgttgcggt
     1141 ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa cactttgacc
     1201 atcgcacctg gtgtggtagt tgtttatgac cgcaataccg tgaccaataa gattttggaa
     1261 gaatacgggc ttcgcttgat taagattcgc ggaagtgaat tggttcgggg ccgtggtgga
     1321 cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt aatagctgtt cggcattcgt
     1381 caatagaaaa tgtaaaaaat agaaagagga aataataaaa tgataaattc agtattccaa
     1441 ggacgcagct tcttagcaga aaaagacttt acccgtgcag agttagaata ccttattggt
     1501 ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc aacaccacta ccttgctggc
     1561 aagaatatcg ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc ctttacaact
     1621 gcggctatcg accttggtgc tcacccagaa tacctcggag caaatgatat tcagttgggt
     1681 aaaaaagaat ctactgaaga tactgctaaa gtattgggac gtatgtttga cgggattgaa
     1741 ttccgcggat tcagccaacg tatggttgaa gaattggcag aattctcagg cgttccagta
     1801 tggaacggtc taactgacga atggcaccca actcaaatgc tcgctgacta cttgactgtt
     1861 caagaaaact tcggtcgctt ggaaggcttg acattggtat actgtggtga tggacgtaac
     1921 aacgttgcca acagcttgct cgtaacaggt gctatccttg gtgtcaatgt tcacatcttc
     1981 tcaccaaaag aactcttccc agaaaaagaa atcgttgaat tagcagaagg atttgctaaa
     2041 gaaagtggcg cacatgttct catcactgaa gatgctgatg aagcagttaa agatgcagac
     2101 gttctttaca cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc agaacgtgta
     2161 gctcttctta aaccttacca agtcaatatg gacttagtta aaaaagcagg caatgaaaac
     2221 ttgatcttcc tacactgctt gccagcattc cacgatactc acactgttta tggtaaagac
     2281 gttgctgaaa aatttggtgt agaagaaatg gaagtaacag acgaagtctt ccgcagcaag
     2341 tacgctcgcc acttcgatca agcagaaaac cgtatgcaca ctatcaaagc tgttatggct
     2401 gctacacttg gtaaccttta tattcctaaa gtataatttt agataataaa ccgtctacca
     2461 acagctatga gggctgcgac taatagcttt agtccggtcc tcttttatgt aatggtaatc
     2521 tattatttct tataaaatat gtgaaaaatc attaaattga aatctaaacg cattctattg
     2581 agtgtgataa aggagaattt atggcaaatc gtaaaattgt agtagctttg ggaggaaatg
     2641 cgattctttc ttctgaccca tcagcaaagg ctcaacaaga agctttagtt gaaacagcta
     2701 agcatcttgt aaaattgatt aaaaatggag atgatctgat tatcactcac ggtaatggac
     2761 ctcaagttgg gaatctcttg ctccaacatt tggcatcaga ctctgaaaag aaccctgcct
     2821 tcccactcga ctcacttgtc gctatgacag aaggtagcat cggtttctgg ttgaaaaatg
     2881 ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct gttgtaacgc
     2941 aagttgtcgt agataaaaat gatccagctt ttgttaactt gagtaaacca atcggtcctt
     3001 tctattcaga agaagaagca aaagcagaag ccgaaaaaag cggagcgact ttcaaggaag
     3061 atgctggccg tggctggcgt aaggtcgttg cctcaccaaa acctgttgac atcaaagaaa
     3121 ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt cgtagctgca ggtggtggcg
     3181 gtattcccgt cgtcaaagaa aacaatggac atttgactgg tgtcgaagcg gttattgata
     3241 aagacttcgc ttcccaacgt ttggcagaat tggttgatgc agacctcttc atcattttga
     3301 caggtgtaga ttatgtattt gttaactaca acaagccaaa ccaggaaaaa ttggaacatg
     3361 tgaatgttgc ccagctggaa gaatatatca aacaagatca gtttgcacca ggtagcatgc
     3421 ttccaaaagt agaagcagct atcgcttttg tcaatggtcg tccagaagga aaagcagtta
     3481 ttacttccct tgaaaatcta ggcgccttga ttgaatctga aagcggaaca attattgaaa
     3541 aaggataagt tgttttacta ataagatgta ttctatttct agtatcttta tatcaaatta
     3601 gaaattattc ttgaaaacat gtacaatatt tcaaaagata ttagtttttt agactttaat
     3661 atggtaaaac aaatataaat agaaagcgtt ttcttgaatg tttatttaag aaagtagttg
     3721 gttttttaca ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa gctaaaaaag
     3781 ggtttaagat gccttcatct tacaccgtat tattgataat cattgctatt atggccgtgc
     3841 taacttggtt tatccctgcg ggggccttta tagaaggtat ttacgaggct catcctcaaa
     3901 atccacaagg gatttgggat gtcctcatgg caccgattcg ggctatgcta ggtactcatc
     3961 cagaggaagg ttcgctcatt aaagaaacga gtgcagcgat tgatgtagcc ttcttcatcc
     4021 ttatggttgg tggtttcctt ggcattgtca acaaaactgg tgctcttgac gtagggattg
     4081 cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt aattttggta ctgatgcctt
     4141 tgtttgccct cggtggtaca acttatggta tgggtgaaga aacaatggcc ttctatccac
     4201 tccttgtgcc agttatgatg gccgttggtt ttgatagcct gactggtgtt gcaattattt
     4261 tgctcggttc tcaaatcggc tgtttggcat ctactctgaa tccatttgcg acaggtattg
     4321 cttcagcgac tgcgggagtt ggtacagggg acggtatcgt acttcgtctg atcttctggg
     4381 ttaccttgac tgctcttagt acttggtttg tttaccgtta tgcggataag attcaaaaag
     4441 atccgactaa gtcactggtt tatagtactc gcaaagaaga tttgaaacac tttaacgtag
     4501 aagaatcttc atctgtagaa tctacactta gcagcaaaca aaaatcagtt ctcttcttat
     4561 ttgtgttgac attcatcttg atggtattga gcttcattcc atggacagac cttggcgtta
     4621 ccatttttga tgactttaat gcttggttga ctggtcttcc agttattggt aatattgtcg
     4681 gttcatctac ttctgcacta ggtacttggt acttcccaga aggcgcaatg ctctttgcct
     4741 ttatgggtat cctgattggt gttatttatg gtcttaaaga agataagatt atctcttcct
     4801 tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt gctcgtggta
     4861 ttcaagttat catgaacgac ggtatgatta ccgatacaat cctcaactgg ggtaaagaag
     4921 gcttgagcgg tctatcttca caagtcttta tcgttgtaac ttatatcttc tatctaccta
     4981 tgtcattctt gatcccatct tcatctggtc ttgccagcgc aactatgggt atcatggctc
     5041 cacttggaga atttgtaaat gtccgtccta gcttgattat cactgcttac caatctgctt
     5101 caggtgtctt gaacttgatt gcaccaacat ctggtattgt gatgggagct cttgcacttg
     5161 gacgtatcaa cattggtact tggtggaaat tcatgggcaa actcgtagtc gctattattg
     5221 tagtgaccat cgcccttctt ctccttggaa ccttccttcc attcctataa aatagtgagt
     5281 gaggtgattc catgaaaata gatataacaa atcaagttaa agatgaattt cttatatcat
     5341 taaaaacctt gatttcctat ccttcagtac tcaatgaagg agaaaatgga acaccttttg
     5401 gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat ttgtcgagac ataggtttca
     5461 ctacctatct tgaccctaaa ggttattacg gatatgcaga aatcggtcag ggagcagagc
     5521 ttctggccat tctctgtcat ttggatgttg ttccatcagg tgatgaagca gattggcaga
     5581 caccgccatt tgaagcaact atcaaagacg gctgggtatt cggacgtggt gtccaagatg
     5641 ataaaggccc ttcgctcgca gctctctatg cagtaaaaag cttgctggac caaggtattc
     5701 agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga ggaaaccctc tggcgctgca
     5761 tggcacgcta caataccatc gaagaacagg ccagcatggg ctttgcacct gactcatctt
     5821 ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa acttcatggc cctggatcgg
     5881 atcaactaga gcttgaagta ggaggcgcct ttaacgttgt accagacaag gccaactacc
     5941 aaggtcccct ctatgaacag gtttgtaacg gtctcaaaga aactggttat gattaccaaa
     6001 ccactgaaca aaccgtaacg gttctcggag tgccaaagca tgctaaggat gctagtcaag
     6061 gtatcaatgc tgtcatccga ctagctacca ttcttgctcc tctccaagaa caccctgctc
     6121 tcagctttct tgcaacacaa gcaggtcaag acggcacagg aagacaaatc tttggtgata
     6181 tagcagatga accttctggt cacctatcct ttaatgtcgc aggtctcatg atcaatcatg
     6241 aacgttctga aatccgtatt gacattcgga ctcctgtctt agctgacaag gaagaactag
     6301 tagagttgct tacaagatgt gcacaaaact accaactccg ctacgaagag tttgactatc
     6361 tagcgcctct atacgtcgca aaagacagta aactcgttag cacactgatg caaatctacc
     6421 aagaaaagac tggcgataac agtcctgcta tttcatccgg tggtgccact tttgctcgca
     6481 ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag acagaacatc
     6541 aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt tatgccgaag
     6601 ccgtctatcg acttgcaact taatcaggca actgtttcta caaaaaaaaa tcgaccgatt
     6661 aatgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtg ttttattatg
     6721 aaattga
//
LOCUS       DAVLTM010000098         6486 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLTM010000098 DAVLTM010000000
VERSION     DAVLTM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.099"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            348..1394
                     /gene="pheS"
                     /locus_tag="VYN16_001689"
     CDS             348..1394
                     /gene="pheS"
                     /locus_tag="VYN16_001689"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1404193.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1394..1903
                     /locus_tag="VYN16_001690"
     CDS             1394..1903
                     /locus_tag="VYN16_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404194.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GIGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1980..4385
                     /locus_tag="VYN16_001691"
     CDS             1980..4385
                     /locus_tag="VYN16_001691"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1404195.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENTVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVIIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQVSLEEKVNA
                     EVR"
     gene            complement(4453..5454)
                     /locus_tag="VYN16_001692"
     CDS             complement(4453..5454)
                     /locus_tag="VYN16_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1404196.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            5601..6004
                     /locus_tag="VYN16_001693"
                     /pseudo
     CDS             5601..6004
                     /locus_tag="VYN16_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6130..6396
                     /locus_tag="VYN16_001694"
     CDS             6130..6396
                     /locus_tag="VYN16_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1404197.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
BASE COUNT         1984 a         1204 c         1486 g         1812 t
ORIGIN      
        1 tcccaatcga tttgttttca tcttatttca atccgctata aagttagata agaataagct
       61 aacaagcaga agtaagatag gtcttgcaag aatggtaaaa atctgataaa ctaagtagta
      121 attgaatagg aatctgaaag actagtagcc caagggaagt atatcaggga ggagagccgt
      181 gactgcaagc tctctatatg aaagctgggt gaattcactt gcgcatggat ttagaaatga
      241 aggtctgact tgtcagatga aaacggatgg taccgcgtgt caacgctccg agtggagttt
      301 ttggcatgtg gttttctttt tatctacgag agactgatgg aggaaatatg tcaactattg
      361 aagaacaatt aaaagcgctt cgcgaagaaa cgctggctag cttgaagcag attactgctg
      421 gaaatgaaaa agagatgcaa gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca
      481 ctgaaatcct caaagggatg aaagatgttt ctgctgagat gcgtccaatc atcgggaaac
      541 acgtcaatga agctcgtgat gtcttgactg ctgcttttga agaaacagct aagctcttgg
      601 aagaaaagaa agtcgcggct caactggcta gcgagagtat cgatgtgacg cttccaggtc
      661 gtccagttgc gactggtcac cgtcacgttt tgacacaaac cagtgaagaa atcgaagata
      721 tcttcatcgg tatgggttat caagtcgtgg atggttttga agtagagcaa gactactata
      781 actttgaacg tatgaacctt ccaaaagacc acccagctcg tgatatgcag gatactttct
      841 atatcactga agaaatcttg ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg
      901 atgcccatga tttttctaaa ggtcctttga agatgatctc gccagggcgt gtcttccgtc
      961 gcgatacgga cgatgcgacc cacagtcacc aattccacca aatcgaaggc ttggtagttg
     1021 ggaaaaatat ctctatggct gatcttcaag gaacgcttca gttgattgtc caaaaaatgt
     1081 ttggtgaaga gcgtcaaatt cgtttgcgtc catcttactt cccattcaca gagccatctg
     1141 ttgaggtgga tgtttcttgc ttcaagtgtg gtggagaagg ctgtaacgta tgtaagaaaa
     1201 caggttggat cgaaattatg ggagccggta tggttcaccc acgtgtcctt gaaatgagtg
     1261 gtatcgatgc gactgtatac tctggttttg cctttggtct tggacaagag cgtgtagcta
     1321 tgctccgtta tggaatcaac gatatccgtg gattctacca aggagatgtc cgcttctcag
     1381 aacagtttaa ataatgatta gaaaagtaga aatggcagat gttgaggtgt tggctaaaat
     1441 tgccaaacaa gcctttcgtg aaacctttgc gtatgataat acggaagagc agttacagga
     1501 atactttgaa gaggcttata gtctgaaaac tttgtcaact gagttgggaa atcctgactc
     1561 tgaaacctat ttcattatgc atgaggagga gatagctggt tttctcaaag tcaactgggg
     1621 aagtgctcaa actgagagag aattagagga cgcttttgaa attcaacgcc tctatgtgct
     1681 acaaaaattc caaggatttg gaataggtaa gcaactgttt gaattcgcac ttgaacttgc
     1741 tacaaaaaat agtttttctt gggcttggct aggtgtttgg gagcataata caaaagctca
     1801 agcgttttat aatcgatatg gttttgaaaa atttagccaa catcatttta tggttggtca
     1861 aaaagtagat acggattggt tactgagaaa gaaattaagg taagaagagg attcttctat
     1921 tgaaatgata ggaaaggaaa agaaccagtg tggcaatggt cattctggag aactaaatta
     1981 tgcttgtatc ttataaatgg ttaaaagaat tggtggacat tgatgtgcca tcacaggagt
     2041 tggctgaaaa aatgtcaacc acagggatcg aggtagagag tgtcgaatca ccagctgctg
     2101 atctctcaaa aattgttgtc ggtgaggtct tgtcttgcga agatgtgcca gagactcacc
     2161 tccatgtttg tcaggttaac gttggcgaag aagaagcccg tcagatcgtt tgtggtgccc
     2221 caaatgtgcg tgctgggatc aaggtcatgg tggctcttcc aggagctcgt atcgctgata
     2281 actacaaaat caaaaaagga aaaatccgtg gtttggagtc acttggaatg atctgttcac
     2341 ttggtgaatt gggaatttct gactcagttg tgcctaagga attcgcagat ggcatccaaa
     2401 tcttgccaga aaataccgtg ccaggtgagg aagtcttttc ttacctagac ttggatgatg
     2461 aaatcatcga actttccatc acaccaaacc gtgcagatgc cctttctatg tgtggagtgg
     2521 ctcacgaagt ggcagccatc tatgacaagg cggtcaactt taaagaattt actctaacag
     2581 aaactaatga agctgcggca gatgcccttt ctgtcagcat tgggacagac aaggcgcctt
     2641 actatgcagc tcgtatcttg gacaatgtga ttatcgcacc aagtccacaa tggttgcaaa
     2701 accttctcat gaacgaaggc atccgtccca tcaataacgt tgtagacgtg acaaactaca
     2761 tcctgctcta ctttggtcaa cctatgcatg cttttgactt ggacacattt gaagggactg
     2821 acatccgtgt gcgtgaagcg cgtgctggtg aaaaattggt gaccttggac ggtgaagaac
     2881 gtgacttgga cgtgaatgac ctagtcatca ctgttgcaga caagccagta gcccttgcag
     2941 gtgtcatggg tggtcaagca acagaaatct ctgaaaaatc tagtcgtgtt gtccttgaag
     3001 ctgctgtttt caatggtaaa tctatccgta agacaagtgg tcgcctgaac cttcgttcag
     3061 aatcgtcttc tcgctttgaa aaaggcatca atgtggcaac agtcaatgaa gcccttgatg
     3121 cggcagctag catgattgca gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag
     3181 cgggtgagct tgatacctct gatgtggaag tttcttcaac ccttgctgat gttaaccgtg
     3241 tcctcggaac tgagctgtct tatgctgatg tcgaagacgt cttccgtcgt cttggttttg
     3301 gtctttctgg aaatgcagac agctttacag tcagcgtacc acgtcgtcgt tgggatatca
     3361 caatcgaagc tgacctcttt gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa
     3421 ctagtctacc aaaagacgat ggtacagctg gtgaattgac tgtgacacaa aaactccgcc
     3481 gtcaagttcg taccattgct gaaggagcag gtttgacaga aatcatcacc tatgctctaa
     3541 caactcctga aaaagcagtt gagtttacgg ctcaaccaag taaccttacg gaactcatgt
     3601 ggccaatgac tgtggatcgt tcagtcctcc gtcaaaatat gatttcaggt atccttgata
     3661 ccgttgccta caacgtggct cgtaagaata aaaacttggc cctttacgag attggaaaag
     3721 tctttgaaca aacaggtaat ccaagagaag aacttccaaa tgaaattaac agttttgcct
     3781 ttgccttgac aggcttggtt gctgaaaaag atttccaaac agcagcagtt ccagttgatt
     3841 tcttctatgc taagggaatc cttgaagccc tatttactcg cttgggactc caagtaacct
     3901 atacagcaac atctgaaatc gctagccttc atccaggtcg tacagcagtc atttcactcg
     3961 gtgaccaagt tcttggtttc cttggccaag tgcatccagt cactgctaag gcttatgata
     4021 ttccagaaac gtatgtagct gagcttaacc tttcagctat cgaagctgcg cttcagccag
     4081 cgactccatt tgtagaaatc accaaattcc cggcagtcag ccgtgacgtt gcccttctcc
     4141 tcaaggcaga agtgactcat caagaagttg tagatgctat ccaagctgcc ggcgtgaaac
     4201 gtttgacaga tatcaaactc tttgacgtct tctcaggtga gaaattggga cttggtatga
     4261 agtcaatggc ttatagcttg accttccaaa atccagaaga tagcttaacg gacgaagaag
     4321 tcgcacgcta tatggaaaaa atccaagtat cgctcgaaga aaaagtgaat gcagaagtgc
     4381 gttaaacgat agtatgatac aaattccccc tctagtttat gaacactaga ggggtgattt
     4441 tatataaaat aattaaaatg tgatttctgt aaaaatacat ctatgatcag agttttgaaa
     4501 acttacaatg tctaaatctc taacatagta gtggttttta ggcaataaga tatgatctat
     4561 cgttgcatta aaaagttttg gactttggct attccaagtt cctctttcaa aaggtggaag
     4621 tgcattcaat gcgtccctat gagagcttat ttttgcaagt gctccatgac gcatagttgc
     4681 attaaaatca cctgcaataa tagcctttgg atattttgaa gccaattgat tatgaatgat
     4741 gtttaagtct tgcttccaga tttccattaa acctggcaga ggaggagcag tatgcaaggc
     4801 aatgatatct ggtatatttt gttttctcga atgtaataca attgtcccga accgtgttgt
     4861 atgaaaagtt ttagcttctg tatagaaacc ataacttttc ttgacaatca cagtcacagg
     4921 agctattcca ctattggtag gtggagaagt gaaaatatca tagttggcca tagaaagtcc
     4981 aacttgatga aacaatagtt tgattctctg gttttcttgc tcacctctga tattggtagc
     5041 tagttcaggg aatatagcca tatcggcatc aaaatggcta aaaattcgct caatatgttg
     5101 tgcttctatt tgattagcga cgttccaagt tactaatttt aaggttttag tcttatttga
     5161 tgcagaagaa agggttttat ctgttccgaa taatagtaat atgagcgata gtaagagcaa
     5221 aatgccagag taaaaccgta tttgtttatt tttcttgtaa aaagataggg aaaaaaatac
     5281 gaaacttaaa atagaagcca tagttgcagt agctaagggg aaggcataaa tctgtgccaa
     5341 gataggtctg gtagatagcc aaatattaag attagaagat ggaaaaaaag aataagctat
     5401 gatagggata acaaagagaa tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg
     5461 agtacctcaa tttgcaatgg ttgctcggag ctaatgtgac tttacttgat tgatattctt
     5521 tttcttgtct tcctcataga ttaagtacaa cccaagtaaa aaagttcaat ttaagattga
     5581 aatgattaaa aggcacagaa atgcaagaca attatactac aaaaggcaaa catttgacaa
     5641 tcgatagccg tcgcttaatc gaacgatgga aaaaagaagg aaaatcaaat agagaaattg
     5701 cctctctact tggaaaagct cctcaaacta tccacactga aatcaagcgt gggacagtcc
     5761 gacaatgtct tggaaaaggg cgcttcaaag agatttattc tgccgactac gctcaacagt
     5821 cttaagaaac taaaggaaaa gattctccac tatacataac caaaaatttt cgcctgaaat
     5881 gatggtgatg gctaaagggg ttaacgtggg aatttcaacc atttactatt ggattcatcg
     5941 tggaaaattg gggttaagca agcaggatct gctttatcct agaaaaggaa aatcgcttaa
     6001 gaaataggct agcaccaact ttaaacccgc tggtcaatct atcgaatcat aattcgctca
     6061 tttttttaaa aatacataga cgtaaaaaaa agagggttaa gtgatgaaga atagtgctat
     6121 tgggagtaat tggaaggatg tccatctgag ctctttagca aggaggaaat ccttgaaagt
     6181 gatatgcgag tggctatcat gagcgagttg attgaggcta ggtatgaaca aggaatcagt
     6241 cagaaaaagc tggaagaagt cagtggaata agccagcctg tcatagctag gatggagaca
     6301 ggagagagca gtcttcagtt ggatacggtc ttaaaagttc taaccagtct aggaaagaca
     6361 ctagcagtcg ttccacttga acaggggaaa agttgactag aatcagttat agtcttttct
     6421 aataacaggt catagtcact tataagaatt actaataaca tgttttagta ttctaattgg
     6481 aatata
//
LOCUS       DAVLTM010000099         6418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLTM010000099 DAVLTM010000000
VERSION     DAVLTM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.100"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            48..443
                     /locus_tag="VYN16_001695"
     CDS             48..443
                     /locus_tag="VYN16_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1404198.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKFFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            454..825
                     /locus_tag="VYN16_001696"
     CDS             454..825
                     /locus_tag="VYN16_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1404199.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            835..3078
                     /locus_tag="VYN16_001697"
     CDS             835..3078
                     /locus_tag="VYN16_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1404200.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QAAGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3285..5060
                     /gene="spxB"
                     /locus_tag="VYN16_001698"
     CDS             3285..5060
                     /gene="spxB"
                     /locus_tag="VYN16_001698"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1404201.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5171..5518
                     /locus_tag="VYN16_001699"
     CDS             5171..5518
                     /locus_tag="VYN16_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1404202.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5635..6418)
                     /locus_tag="VYN16_001700"
                     /pseudo
     CDS             complement(5635..6418)
                     /locus_tag="VYN16_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1900 a         1221 c         1439 g         1858 t
ORIGIN      
        1 tatactgatg agtgtaattg acaaatgtag attttggagg tatggtaatg cagatttcag
       61 atgcagaatg gcaggtcatg aagattattt ggatgcaggg ggagcagacc agtacagatt
      121 tgattagggt tttggcagag cggttcgact ggtccaagtc aaccattcaa actcttttgg
      181 ctcgtttggt tgagaaagag tgtctgactc ggaaaaaaga aggcaagttc tttgtttatt
      241 cagccctttt aactctagac caaagtcggg atttacttgt ccaagatatc aaagacaagg
      301 tttgttcccg taggattagg aatttgttgg ctgacttgat tgtagaatgt gaatttactc
      361 agactgactt ggaagacttg gaagctgtga tttcagagaa gaaatcaagt gctgtaacag
      421 aagtaagatg taattgtatg taaaggagac gtcatgttaa atagtattgt aaccattatt
      481 tgtattgccc ttatcgcgtt tatcttgttt tggtttttca aaaagcctga aaaatctgga
      541 caaaaagccc agcaaaaaaa cggataccaa gagattcgag tggaagtcat gggaggctat
      601 actcctgagt tgattgtcct caagaaatca gtgccagccc gcattgtctt tgaccgcaag
      661 gatccttcac catgtctgga tcaaattgtt tttccagatt ttggtgtaca tgcgaacctg
      721 ccaatggggg aagagtatgt agtggaaatc acgcctgaac aggctggaga gtttggcttt
      781 gcttgtggta tgaacatgat gcacggcaag atgattgtag agtaggtgga gactatgaca
      841 gaaattgtga aagcaagctt agaaaatggc attcaaaaaa tccgtatccg agctgaaaaa
      901 ggctatcatc cagcccatat ccagcttcaa aagggaattc cagctgagat tacctttcat
      961 cgtgctactc cttcaaactg ttataaggaa attctgtttg aagaagaagg tatcttggaa
     1021 ccaatcggcg tagatgagga gaaagtcatt cgttttacac ctcaagaatt agggcgacat
     1081 gaattttctt gtggcatgaa gatgcaaaag ggaagctata tagtcgttga gaagactcga
     1141 aaatctctat ctctcctgca acgtttttgg attactagta tctttactgt gcctcttgtg
     1201 attctcatga ttgggatgtt gacaggtagc attagtcatc aagtcatgca ttggggaacc
     1261 tttctagcaa caacgcctat tatgttagtt gcaggtaagc catatatcca gagtgcttgg
     1321 gccagtttta aaaagcacaa tgccaacatg gataccttgg ttgcgctggg aactctagtg
     1381 gcttatttct atagcctagt tgctctcttt gctggtctcc ctgtttactt cgaaagtgct
     1441 ggatttatcc tctttttcgt tcttttggga gcagtttttg aggaaaaaat gagaaaaaat
     1501 acgtcccaag ctgtggagaa attactggac ttgcaagcta aaaccgcaga agtcttgagt
     1561 gatgatagtt atgtccaagt tcctttggaa caagtcaagg taggcgacct gattcgagtg
     1621 cgtcccggtg aaaagattgc tgttgatggt gtcgtagtag aaggtgtctc tagtattgac
     1681 gaatccatgg tgacaggtga gagtctgcct gtggacaaga cagttggaga tactgtcatt
     1741 ggctcaacca tcaatcatag tggaacgctt gtctttagag cagaaaaagt tggctcagag
     1801 actgttttgg ctcagattgt ggattttgtg aagaaagctc agacaagtcg tgcgccgatt
     1861 caggacttga cggataagat ttcagggatt tttgtcccag tagttgtcat tttaggaatc
     1921 atgacctttt gggtttggtt cgtcttgctc agggatagtg tggtcgtgct tggagctagc
     1981 tttgtgtcct ctcttctcta cggagtggcg gttttgatta tcgcctgtcc ttgtgccttg
     2041 ggacttgcaa caccgacagc ccttatggtg gggacaggac gtagtgccaa gatgggggtt
     2101 ctcctcaaaa atggaactgt cttacaggaa atccagaaag ttcaaactct tgtctttgat
     2161 aagaccggga ctttgacgga agggaaacct gtggtaacag atatcatcgg cgacgaagta
     2221 gaagtgtttg gattggcagc ctccttggaa gatgcttctc aacacccact ggctgaggct
     2281 atcgttaagc gagcgagtga agctggactt gagtttcaaa ctgttgaaaa ttttcaggcc
     2341 ttgcacggga aaggtgtttc agggcgaatc aatggaaaac aagttttact tggaaatgct
     2401 aaaatgctgg atggcatgga tatttctaat acttatcaag ataaactaga agaactagaa
     2461 aaagaagcta agacagttgt gtttttagct gttgacaatg aaatcaaagg cttgcttgct
     2521 ttgcaagata ttcctaagga aaatgctaag ctagccatca gtcagctaaa aaaacgtggt
     2581 ctccgaacag tcatgctgac aggagacaat gctggtgtgg cgcgtgctat tgcagatcaa
     2641 atcggaattg aagaggtcat tgcaggcgtc ttgccagaag aaaaagccca tgaaatccat
     2701 aaactgcaag cggctggcaa agtagccttt gttggggacg gtatcaatga cgctcctgcc
     2761 cttagtgtag cagatgtggg aattgctatg ggtgctggaa cagatatcgc catcgagtca
     2821 gcagatttgg tgttgacaac caataatctt ttaggagtgg ttcgtgcctt tgatatgagt
     2881 aagaaaacct ttcatcgaat tctactcaat cttttctggg cttttatcta caatgttgtc
     2941 ggaattccga ttgcagcagg agtcttttca ggtgttggac tggctctcaa cccagaattg
     3001 gcaggtctag ccatggcctt tagttctgta tctgttctga ccagttccct tctgttaaat
     3061 tttagtaaaa tagactaaaa gtaatgacta gatttctttg ttataaaaca gaaatgacaa
     3121 cactttcaaa actgtattag aatagagaat agagagtttt gagcagattt ttagaaaagt
     3181 cagcataaat atgatacagt ggaatagtaa aaatttggag aacgtttcca attctatgta
     3241 atcgtattct ccaagtttaa aaaaattgaa ggagagttat cattatgact caagggaaaa
     3301 ttactgcatc tgcagcaatg cttaacgtat tgaaaacatg gggcgtagat acaatctacg
     3361 gtatcccatc aggaacactc agctcattga tggacgcttt ggctgaagac aaagatatcc
     3421 gcttcttaca agttcgccac gaagagacag gtgctcttgc agcggttatg caagctaaat
     3481 tcggcggctc aatcggggtt gcagttggtt caggtggtcc aggtgcgact cacttgatta
     3541 acggtgttta cgatgcagct atggataaca ctccattcct agcgatcctt ggatcacgtc
     3601 cagttaacga attgaacatg gatgctttcc aagagcttaa ccaaaaccca atgtacaacg
     3661 gtatcgctgt ttacaacaaa cgtgtagctt acgctgagca attgccaaaa gtaattgacg
     3721 aagcctgccg tgctgcagtt tctaaaaaag gtccagctgt tgttgaaatt ccagtaaact
     3781 tcggtttcca agaaatcgac gaaaactcat actacggttc aggttcatac gaacgctcat
     3841 tcatcgctcc tgctttgaac gaagttgaaa tcgacaaagc tgttgaaatc ttgaacaatg
     3901 ctgaacgccc agttatctat gctggatttg gtggtgttaa agctggtgaa gtgattactg
     3961 aattgtcacg taaaatcaaa gcaccaatca tcacaactgg taaaaacttt gaagctttcg
     4021 aatggaacta tgaaggtttg acaggttctg cttaccgtgt tggttggaaa ccagccaacg
     4081 aagtggtctt tgaagcagac acagttcttt tccttggttc aaacttccca tttgctgaag
     4141 tttacgaagc attcaagaac actgaaaaat tcatccaagt cgatatcgac ccttacaaac
     4201 ttggtaaacg tcatgccctt gacgcttcaa tccttggtga tgctggtcaa gcagctaaag
     4261 ctatccttga caaagtaaac ccagttgaat caactccatg gtggcgtgca aacgttaaga
     4321 acaaccaaaa ctggcgtgat tacatgaaca aactcgaagg taaaactgag ggtgaattgc
     4381 aattgtatca agtttacaat gcaatcaaca aacatgctga tcaagacgct atctactcaa
     4441 tcgacgtagg taacactact caaacatcta ctcgtcacct tcacatgaca cctaagaaca
     4501 tgtggcgtac atctccactc tttgcgacaa tgggtattgc ccttcctggt ggtatcgctg
     4561 ctaagaaaga caatccagat cgccaagtat ggaacatcat gggtgacgga gcattcaaca
     4621 tgtgctaccc agacgttatc acaaacgttc aatacgacct tccagttatc aaccttgtct
     4681 tctcaaatgc tgagtacggc ttcatcaaga acaaatacga agatacaaac aaacacttgt
     4741 ttggtgtaga tttcacaaac gctgactacg ctaaaattgc ggaagctcaa ggagctgttg
     4801 gattcacagt tgaccgtatc gaagacatcg atgcagttgt tgcagaagct gttaaattga
     4861 acaaagaagg taaaactgtt gtcatcgatg ctcgcatcac tcaacaccgt ccacttccag
     4921 tagaagtact tgaattggat ccaaaacttc actcagaaga agctatcaaa gccttcaagg
     4981 aaaaatacga agcagaagaa ctcgtaccat tccgtctctt cttggaagaa gaaggattgc
     5041 aatcacgcgc aattaaataa ttcctctcgc cgaaaatcaa atatgaaact tgtagacttt
     5101 attctttgat tttcatagag cagaacttta aaagatcgga gcaatccggt ctttttatgg
     5161 aggagagtaa atgaatttaa atcaattaga tattatcgtt tcaaatgttc cccaagtctg
     5221 tgctgacttg gagcatatct tggataaaaa ggcagattat gctgatgatg gttttgctca
     5281 gtttacgatt ggtagtcact gccttatgtt gtcacaaaat catttggttc ctttggaaaa
     5341 ctttcagtca ggaatcatta ttcatatcga gattgaggat gtagaccaga actacaaacg
     5401 gttgaacgag cttggtataa aggttttaca cggaccaact gtaaccgatt ggggaacaga
     5461 gtccttatta gttcaaggac ctgctggtct agtgcttgat ttttatcgta tgaaataggt
     5521 cgcgtagtca tttgacgtta accttagcta tttttaaaat agaaattgag aatatgaact
     5581 attgaaagat cggagcaatc cggtcttttt atataaagaa attgcatata atactcatct
     5641 gctttcaaaa agcattctag tccatctccg attaacgatg gactttatca cctccttctc
     5701 caatccttgt atgacatctt ggagttgatt catgacatct tccaaagttc gaaaggcttt
     5761 attcttaaat ccacgtttac gaatctcttt ccacacttgt tcaatgggtt catctctggt
     5821 gtgtatggag gaataaatgc aaaaccaata ttagtcggaa tctttaaggt acttgattta
     5881 tgccatatag cattgtctat aacgagtaaa aaataatcat atggataagc ttgtgaaagc
     5941 tcttctaaaa aggcgttcat ccactcagta ttacatctac cagctattaa gaaaaatgat
     6001 tcgcctgtat gggcatcaac agctccataa caatagcgaa attctcgtat atagtgacta
     6061 tggacatgtg gacctactcc tattggagac caacaagatc ccagtttact gattctaccg
     6121 aaaccagctt catcttggta catcaagcga accttatgaa aaggtctact ggttttaaag
     6181 cgctttcttg tcttcttgaa ttgagatttt atttttagat gcgacaatgg tttgagcgtc
     6241 tgctttctta ggatgttctg gacgtggcgt aatatttcgc caaccatggc gcttcaacag
     6301 ttgatagaag gcatcacgtg tgtaggaacg acctaactcc tttttataag cctgaaataa
     6361 ggcatcaatt gtaacaaatt ctcctgcctc tgtagccttc aaatggcgag caagaaag
//
LOCUS       DAVLTM010000100         6406 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLTM010000100 DAVLTM010000000
VERSION     DAVLTM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.101"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(71..2329)
                     /locus_tag="VYN16_001701"
     CDS             complement(71..2329)
                     /locus_tag="VYN16_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1404203.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2558..2788)
                     /locus_tag="VYN16_001702"
     CDS             complement(2558..2788)
                     /locus_tag="VYN16_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1404204.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            2978..3661
                     /locus_tag="VYN16_001703"
     CDS             2978..3661
                     /locus_tag="VYN16_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1404205.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3661..4395
                     /locus_tag="VYN16_001704"
     CDS             3661..4395
                     /locus_tag="VYN16_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1404206.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGISLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLRKVL"
     gene            4549..5406
                     /locus_tag="VYN16_001705"
     CDS             4549..5406
                     /locus_tag="VYN16_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1404207.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKIAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDSAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMVPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLTSHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5548..>6406
                     /locus_tag="VYN16_001706"
     CDS             5548..>6406
                     /locus_tag="VYN16_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1404208.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSK"
BASE COUNT         1802 a         1213 c         1443 g         1948 t
ORIGIN      
        1 aagaaattac agttatttta gtaaaaaaag aagcagaaaa atttcatttt ctactccttt
       61 tctataggat ttatttttct ttttcagaac ttttaacttc agcttttttg gcagatttaa
      121 tctgaatgtt tcccttgcta gtcataactg ctttgagatc tttttcgctt ggattttcaa
      181 ggtagtagtc agtgattgtg tcctcaatat agtcttgaat agtccgacga agtgggcgtg
      241 ctcccatttt tggatcataa cctaggtcaa ccaacttttc cttgacctta tcagttacat
      301 ccaaacgaat gttgttgcta gagaggcgct tgttaacatc tgctagcatg agctcgacaa
      361 tctgaaggag gttatccttg ctgagagcct taaattcgat aatgccatca aaacggttca
      421 taaactctgg gctaaagaag ttaccgagtt caccgaggac agaattggta cgtccttctc
      481 tagcagcacc aaatccaacg ctagcttcgg tctttcctgt acctgcattt gaggtcatga
      541 taatgatggc atccttgaag ctaacggtgc gtccttgccc gtctgtcaaa cgaccatcgt
      601 ccaagacttg aagaaacatg tgcataacat ctgggtgagc tttttccact tcatcgagaa
      661 ggatgagaga atatggattg tggcgaactt tttcagttaa ttgaccagcc tcatcatagc
      721 caacataacc tggaggggcg ccgaccaact tagccacact atgtttttcc atgtattcac
      781 tcatatcaaa gcgaatcata ctatcagcag aaccaaaaag ttcgatagcc agttgtttgg
      841 aaagttctgt cttaccgaca ccagttggcc caacgaagag gaagcttccg attgggcggt
      901 taggggtacc aagtccgaca cgattacggc gaatagcctt ggcaatctta tcgactgcat
      961 catcttggcc aataacatga gacttgagat cttcggctag atggatgagt tgagattgtt
     1021 ctttctcttt caaatcacca acagggatat tggttttctg ctcgataatg tgctcaatag
     1081 ttttctcgct gatgatagga gtatcctggt ctgtgatctt tttcttttgc atttccttat
     1141 acttggcaat ctggtcgcgg aagtaggccg ccttctcaaa atcttcttct cgtgtagctt
     1201 gagacttgag attttcagcc tcaatcaagc gctgatcaat tactttagga tccacaaaat
     1261 tcaaggtcaa gttcatctta gaaccagctt catctaggag gtcaatggcc ttgtcaggca
     1321 agaagcgatc ttggatgtag cgattggaaa gagttgcagc tgcttcaatc gcagcatcgg
     1381 tatattgaac gtggtggtaa tcttcgtatt tcttttgaat ccctttgaga atagtgattg
     1441 tttcatccac cgttggttca tcgactttaa caggctgcat acgacgctcg agggcagcat
     1501 ccttttcaat gatacggtat tcattgaggg tagtagcacc gactagttgc agttctccac
     1561 gagcaagggc tggcttgagg atatttcctg cgtccatatt accatcactc gcagaaccag
     1621 caccaacaat ttcatggatt tcatcgataa agaggatgat gtcttcacgt ttgcgaattt
     1681 cttccatgag tttttgcatg cgttcttcaa attgtcctcg aatccccgtt ccttgaacta
     1741 agctaaccac atccagacgg atgacttgtt taccttggag tttatgtggc acatcgccat
     1801 cgacaatttt ctgagctaga ccttcgacaa cggccgtttt tccgacacca ggttcaccga
     1861 taaggacagg attattcttg gttctacgat tgagaatctc gatgacacgg ataatctcat
     1921 cgtcgcgccc aataacgggg tcaatgtctc cacgacgggc aatttcagtt acattaatac
     1981 caaattcttc cagcaggcct ttttcttggc taggtggcgg agtttgagca gatccacgat
     2041 tttgggaacc ataaccgccg tttccaccgt aacctccacc tgattgggtt gggggaatag
     2101 gaggagtatt gctagaaggt ctgaaattgt ttagatcatt gaagaaatca ccaaagggat
     2161 cgaagtcacg attgttcaga tccgtcatac ctttgaagag gctattgtta ggatctgttt
     2221 tgataatctt atagcagttt tgacagaggt caatttgttt ttgttttcca ttgagattgg
     2281 tgtaaagatg aattgttgag tcgttaattt tacagttttg acaaagcata catctacctc
     2341 atttctttct ttagcctgac ttgtttaaag gtcaaaaata gtcaaattct atactctcat
     2401 tataacagga ttggggtgta aagcaagtaa aaagattgaa gctttgagaa gttgttacaa
     2461 tgaaaatttt tgtgaatttg gactataaaa aaatcctatt tgtgagacaa aaaggatttt
     2521 acttagacat gcagggacaa tcccagcttg ttttagatta gtagagaagt tcgtaaattt
     2581 ccattgcgat taagtcaatg ctatcaaacg tatacgtttc gcgatcttct acgtcacgaa
     2641 gggtaaagat tgggccgttt tcttcgtcgt ggctgtacgc aacttctgca acaacaactc
     2701 cttcgcgttc aaaattacgt tttaaatttc cgccatcgtt tgccattgct tcaaggcggt
     2761 tgatgattct aaccaaatgt gattccattt tgattctcct tcatttctta tcgttactat
     2821 tttaccataa aggaggccaa cttgactaga aaaaactaag atttctcgct aattctgcta
     2881 gtttaaagat ttttaaataa attggataaa aaatttaaat tttaattcaa aacatgttat
     2941 aatcatacag tattctattt tagaaagcag tgtgactatg aatttttctt ttttacctaa
     3001 gtatttacct tattttaact atggggctgt tgtgacgatt cttatttcta tctgtgttat
     3061 ctttttggga actattttgg gtgttgtctt ggcttttggg caacgttcaa agtttaaacc
     3121 gcttgtttgg ttggccaact tgtacgtttg gattttccgt gggacaccga tgatggttca
     3181 aattatgatt gcctttgctc ttatgcatat caatgctccg actattcaga ttggaatttt
     3241 aggtgttgat ttttcgcgtc tgattccagg gattttgatt atctctatga atagtggtgc
     3301 ttatgtttcg gagactgttc gtgccggaat caatgcggtt ccaaaaggtc agctagaggc
     3361 ggcttattcg ctagggattc gtcctaaaaa tgcgatgcgt tatgtgattt tgccacaagc
     3421 agtcaaaaat atcttgccag cattggggaa cgaatttatc accattatca aggacagctc
     3481 cctcttatca gctattgggg tcatggagtt gtggaatggg gctacaacag tttctacaac
     3541 aacctatcta cctttaacac cacttttatt tgcagcattt tactacttga ttatgacctc
     3601 tattctgaca gtagccttga aagcttttga aaaacatatg ggacaaggag ataagaaata
     3661 atgacagaaa ccttgataaa aattgaaaat ttacataaat cttttggaaa gaatgaagta
     3721 ttgaagggca tcagcctcga gattaaaaga ggagaagttg tcgttatcat cggtccttca
     3781 gggagtggga aatctacctt gcttcgctct atgaatttgt tggaggaagc aaccaagggg
     3841 aaggttatct ttgagggagt cgatattacg gacaagaaga atgacctgtt tgccatgcgt
     3901 gagaagatgg gcatggtttt ccaacaattt aacctctttc ctaatatgac tgtgatggaa
     3961 aatattacct tgtcacctat caagactaaa ggtgaaagca gggaagttgc tgagaagaga
     4021 gcccaagagc ttttggaaaa agttggtttg ccagataagg cagacgctta tccacagagt
     4081 ctgtcgggtg ggcaacaaca acggattgcc atcgcgcgtg ggttggctat ggaaccagat
     4141 gttttgctct ttgacgaacc aacttcagcc ttggatcctg aaatggtagg tgaggtattg
     4201 gctgttatgc aagaccttgc caagtcaggg atgactatgg ttatcgtaac acatgagatg
     4261 ggatttgccc gtgaggtggc agatcgtgtt atctttatgg cagacggtgt ggttgttgaa
     4321 gatgggacac ctgagcagat ttttgaacaa acccaagaac aacggactaa agatttcttg
     4381 agaaaggttt tataatctat tttaaaattt caagacaaga ttagtgaaaa gttcaaccag
     4441 agctttttct tatagtttga aactatagga taacctagga aagaagtgtt agagggcact
     4501 agcagttttt ggtataatgg aagatatttg aaagaaaaga gaagtgatat gacacagatt
     4561 attgatggaa aagctttagc ggccaaattg caggggcagt tggctgaaaa gattgcaaaa
     4621 ttaaaggaag aaacagctct agtgcctggt ttggtagtga ttttggttgg ggataatcca
     4681 gccagccaag tctacgttcg caacaaggag aggtcagctc ttgcggctgg ttcccgtagc
     4741 gaagtagtgc gagttccaga gaccattact caagaggaat tgttagacct gattgctaaa
     4801 tataatcagg attcagcttg gcatgggatt ttggtccagt tgccattacc aaaacatatc
     4861 gatgaagagg cggttttatt agccattgac ccagaaaagg atgtggatgg tttccatccc
     4921 ctaaacatgg gacgtctttg gtctggacat ccagtcatgg ttccctcgac acctgcagga
     4981 attatggaaa tgtttcatga atatgggatt gacttggaag gtaaaaatgc ggtcgtaatc
     5041 ggtcgttcaa atatcgttgg aaaacctatg gctcagcttc ttttggctaa aaatgcgaca
     5101 gtaaccttga cccactcacg aactcataat cttgccaagg tggctgctaa agcagatatt
     5161 cttgtagtcg caatcggccg cgccaagttt gtgactgctg actttgtcaa accaggtgcg
     5221 gttgtcattg acgttgggat gaaccgagat gaaaatggga agctctgtgg agatgttgat
     5281 tatgaggcag ttgcaccact tactagccat atcacgccag tccctggagg tgtcggtcct
     5341 atgaccatta ccatgctgat ggagcaaact tatcaagcag cacttcggac attggataga
     5401 aaataagaga aacattcgat taatagagtg tattttctat agctatatct aaaatggcag
     5461 aaatgaatat taaattttag atataagatt acaaaaggag gtctgcgtct cctttttatt
     5521 gtataataaa agtgagagga aaaaaggatg aaagtgatta atcaaacctt attagaaaaa
     5581 gttattattg aacgttctcg ttcaagtcat aaaggagatt atggtcgtct gctgctgctt
     5641 ggtggtactt atccttatgg aggtgccatc atcatggctg ctttagcagc tgtaaaaagc
     5701 ggtgcaggat tggtgaccgt tggaacggac agggaaaata tcccggctct gcacagccat
     5761 ttacctgagc ctatggcctt ttctcttcaa gaccagcaat tgttaaaaga gcaattggag
     5821 aaggcagaag ttgtcttgct ggggcctggt ttacgagacg atgcttctgg agaaaatcta
     5881 gtaaaacagg tctttgttaa tttaagccaa aatcagattt tgattgtaga cggaggtgcc
     5941 ttgaccattc ttgctaggac aagtttgtca tttccctcaa gccagcttat cctaactcct
     6001 caccaaaaag aatgggaaaa attatctgga attacgattg aaaagcaaaa ggaagatgca
     6061 acggctagtg ttctgacttc cttccctcaa ggaacaattt tggtggagaa aggcccagct
     6121 actcgtattt gggaagttgg ccaatctgat tattaccagt tacaggttgg tggtccttat
     6181 caggcgactg ggggaatggg agatactctg gctgggatga tcgcaggatt tgtaggccaa
     6241 ttccgacagg ccagtctcta cgagcgtgtg gcagtagcga cccatcttca ttcagccata
     6301 gcccaagaac tatctcaaga aaattatgtg gtcttaccga cggaaatcag caggtatctt
     6361 cccaaaataa tgaaaattat ttgtcaacaa gagagggtaa gtaaag
//
LOCUS       DAVLTM010000101         6352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLTM010000101 DAVLTM010000000
VERSION     DAVLTM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.102"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(807..2057)
                     /gene="ilvA"
                     /locus_tag="VYN16_001707"
     CDS             complement(807..2057)
                     /gene="ilvA"
                     /locus_tag="VYN16_001707"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1404209.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2107..2463)
                     /locus_tag="VYN16_001708"
     CDS             complement(2107..2463)
                     /locus_tag="VYN16_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1404210.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(2701..2937)
                     /locus_tag="VYN16_001709"
     CDS             complement(2701..2937)
                     /locus_tag="VYN16_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404211.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(3044..4066)
                     /gene="ilvC"
                     /locus_tag="VYN16_001710"
     CDS             complement(3044..4066)
                     /gene="ilvC"
                     /locus_tag="VYN16_001710"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1404212.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(4132..4608)
                     /gene="ilvN"
                     /locus_tag="VYN16_001711"
     CDS             complement(4132..4608)
                     /gene="ilvN"
                     /locus_tag="VYN16_001711"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1404213.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4601..6301)
                     /locus_tag="VYN16_001712"
     CDS             complement(4601..6301)
                     /locus_tag="VYN16_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1404214.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTVEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         1894 a         1436 c         1050 g         1972 t
ORIGIN      
        1 aaaaaaaaca tatattcaac gaaaataatt agttcagaat tttcttactt cctttttaaa
       61 tccgataata tataggtttt gctagcctct acaccataaa tatttgctaa taaaaatact
      121 gtattattac cctcttaagg tgcgaaatta ttcaagttct taaaaaatgc ttctctattt
      181 ccgtaagata gcttatttct tcgatttaag aactgaagat gatagttcta acctttagaa
      241 atccaagcct tcactagctc aaaaactcct atctttcttc ctcaataaat catctgagag
      301 ataggtattc cttatgtcct tcaaattcat cactcctttg gatattgagg tgctagccta
      361 gaagcagaca cccgattatc taaaccatcc ttaatagaga tgacaagaca gataagctca
      421 gttaattact tatatatctt tttaatatgg tatatttgtc aacaaaaaga aatcaaatca
      481 taccttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcaa gttgctcaaa
      541 acactgtttt gaggttgcag atagagctaa cgtggtttga agagattttc gaagagtatt
      601 aaagacagtg tgactatgct tgtcaataga taggaaatca aaatgaagga tggaaatctg
      661 agccaaacat cataagtgct ataatgaaca gaagtaagaa ttgcacgata agttttctaa
      721 aaccatagga atcgtcccct ttcttcatct tcattatagc atatatacat cagttgtaca
      781 aataatatga tattttttat tagtcctcag acaagcatat tataaagcgt ttcattacca
      841 tttaagttaa tataagctgg atcaaaacct tccattctac gaatcaaacc tgcataatca
      901 tgcttatctg ctaaagcgat cccaattaat actgggcctg ttcccttgct agctcgtttg
      961 atatactcaa aacgtgtgat atcatcattt ggccccagga tatcatttac aaactcacgc
     1021 aaagctcctg gacgttgtgg gaaattgacc acaaaataat gtttgatacc atcataaatc
     1081 aaggcacgct cttccatttc tggcatacgg ttgatatcat tatttcctcc agaaatgata
     1141 caacaaatgg ttttcccctt aatatattca gctaaaacct ctaaagaggc gatactagct
     1201 gctccagcag gttctgcgac tatcccttgc ttagagtaaa ggtcaatcaa ggtttcagaa
     1261 atcaatccct catcgacacc taccaaagtt tgaatatgtt gacgagttgc ttcataggtc
     1321 aactgaccta ccttttgcac agcaatccca tccgcaaatt tatcaatttc cttgagtttg
     1381 actggacctc cagcctcaaa ggcagctttc atggaacgcg ctccattcgc ctctactccg
     1441 ataacctcaa tctctggact tgtttccttg atataggtag aaaccccggc aatgagaccg
     1501 ccaccaccaa caggaaccaa gacagcatca aaatcaatcg attcttttcg agcttcttct
     1561 aaaatctcat aagcaactgt tccttgacct gcttgaacat gagcatcatc aaaaggatca
     1621 ataaaggtac gattttcaga gactgtaaat tcttgagctg ctttggctga ggcatcaaag
     1681 gtatctccaa ctagtttaat agttacaaaa tccccaccaa aaaagcgaac ctgaccaatc
     1741 ttttgttgtg gcgtagtaat gggcataaag atagtagcag gaattttcat ttcattacaa
     1801 gtataggcta ctccctgcgc atgatttccc gcagaagcgc agactacccc acgttcacgt
     1861 tcttccttgc tgagctggga aatggcataa taggcaccac gaattttaaa ggagcgaaca
     1921 cgctgggcat tttctttttt caaataaatc ttagcaccat acttctccga taaataatga
     1981 tcgtaatcca gtggagtatt cacaaccaca ccgttcaaga ccttgtgagc cttgattata
     2041 tcttttgaac ttaacatata aaacctcttt ttctataaaa aacagtcact aatgactgtt
     2101 ttttagttat tcatctccga aaacttcagt aacactttca tggtatttat gagccatacg
     2161 aatcgaaaac tctttaggaa gttcataacg atttgtggaa atccaatcat ataaccattc
     2221 taccaaactt tcttcactat cattacgatg gaagataaaa tagtttaaaa tttctttata
     2281 ttctacatca ttcaaatcca acgtgtctac aaattgacta cgaggaacaa aattagaatc
     2341 taattttctt agttgatctg tcaattctcc caattgtaaa tccaactgaa aaaatagttt
     2401 ttgcagttgc ctatcagttt catcacatcc tgtcttgata gaaaacaaat tggtaatatt
     2461 catatgattt caacccctaa ataagtacat ctaatatccc acgaatagct aaagctaaag
     2521 ttttcgcagt ccagtcattt aaacccgatt ctttcaatcc agatgcaata atttgctcta
     2581 tttgatataa accgctatct atcgtctcat ttattctgcg aagaggacct tcaatatagg
     2641 ataaagcttc tccgaatcta acagctgatt taggtcctaa tgatttacca actatatttg
     2701 ttaatgtttt ttatttctca aaatccattt cgctatataa cgagcagatt tagtcgcacc
     2761 agctctctca aacggagaat gaatttcaag accttcttct ctagctattt gtgccaattt
     2821 gaagaattgt tcaaaggtca tatgcaacct accatcttca aaattagcat catcataaat
     2881 gttaattaca gcattttcat catacgacca atctgcttta ttttggtctg ctagcactat
     2941 taccgacggt gtaattacat tatacaacta gaaccaataa taacgatgca atttttctca
     3001 taatcttttt cataaaatca tctccagcgc tatatatttc taattagtta tagattttga
     3061 atgcatcatc gtcgttttta ccaacgaatg gcattgcttt acgcaattct gcaccaactt
     3121 tttcaatttc aaggttagct gcttgttcac ggtaagcagt caattttgga cgtccagctt
     3181 tatagtcatt tacaaagtca tttgcaaatt taccattttg gatgtctgcc aagacagcct
     3241 tcatattttc tttaacttgt tcagtgatta cacgtggacc tgatacatag tcaccgtatt
     3301 cagcagtgtt tgaaatagat tgacgcattt tcttgaatcc accttcgtag atcaagtcaa
     3361 cgatcaattt catttcgtga agaacttcaa agtaagccaa ttctggagcg taacctgctt
     3421 ctgtcaagac ttcgaaacct gcttcgataa gggcagtcaa accaccacaa agtacagctt
     3481 gttcaccaaa caaatcttct tcagtttctt ctttgtaagt tgtttcaaga agacctacac
     3541 gagccgctcc aacaccttta caccagtcca tagcaatgtt tttagcattt cctgttgcat
     3601 cttggtatac tgcataaaga gctggaacac caaatccttc ttcgtaagta cgacgtacca
     3661 agtgtcctgg tcctttagga gcacacatga agacatctac atccgcagga actttgataa
     3721 attcaaagtg gatgttgaaa ccatgggcaa atccaactgc gtttccagct tccaagtttg
     3781 gagcgatttc tgcttcgtac aattcttgtt gaatttcgtc tggcgccaag atcatgataa
     3841 catcagccaa cttagtagct tctgctactg tgtaagtatc aaatccatct tcttttgctt
     3901 tatcaaaaga tttacctgga cgtacaccta taataacgtc acgacctgaa tcacgcaagt
     3961 tttgagcatg cgcatgccct tgtgaaccat aaccgataac ggcgattttt ttaccgtcaa
     4021 gtgctgctac tttaacatct ttttcatatt ccatttgaac tgtcatagct tttctctctt
     4081 ttctattatt tattgccttt taggctggtt taataaattt aagttggatt tttaatcgcg
     4141 ggtaaatcca gttgcacccg ttcgagcaat attgcgaata ccgtatgggc gaatgactct
     4201 caatagggct tcgctctttt ctgcatttcc cgtcatctga atggtaatcg agcttggcgc
     4261 tacgtctacc actgttgcac ggaaaggttg aataatcgct aaaatctcag ctctcttctc
     4321 agctggcgct gacatcttaa ctaaaatcac ctcgcgctcc aaatgaggct tgtctgtaat
     4381 atctcgaatg cgaatcacat caatctgacg attgagctgt ttgatgattt gctccacttc
     4441 atcatgagaa gcaacatcaa taataatagt gatacgcgat acattcggat cttctgttgc
     4501 tccaacagag atgctttcaa tattaacctg acgacgagat aggacacctg taaagcgatt
     4561 gaggactcct gaacgatttt gtagttttgc tgttaacatt ctacgcatgg aactgcaccc
     4621 ccaacatctc atgattactc ttaccagccg gtaccattgg taacacctgt tccttacgag
     4681 aaatatctac ctcaattagc ataggaacat cctcagtgat gacttcaagg tcttgagcca
     4741 aggtctcagg attgtcaaac ttatagtttt taataccata agcctgcgcc atcaattgga
     4801 aatcaggaag ggtatcaaag accgactctg atgttctgcc ttcatagaag gattcctgcc
     4861 actggcgaac cattccgagt gaatgattgt tcagcataac caccttgatt ggcaccttgt
     4921 aaatattcaa aatagccaac tcctggttgg tcatttggaa accaccatcc ccaacaaaca
     4981 agactacttc cttatctggg ttagcaattt tagcaccgat tgctgctgga attccaaagc
     5041 ccattgttcc caaaccacct gaagtcacta actgacgttc attttggtag ggataatact
     5101 gagctgtcca catttggtgt tgaccaacgt ctgttaccac aatggcatct ccattcgtca
     5161 attcaccaat tcgttcaata actgcttgcg gttgaaccac acgctctttc ttgtcataag
     5221 aacgaacacg attcttgtct ttagtgactt tctcaatcca cttttcagta ttgttgtgaa
     5281 ctgttggttc tgctagcaac atttgcaagg ccttcttagc atctccaact acaggaatgt
     5341 ctgcactgat aatcttgcca atctcagctg ggtcaatatc aatgtgggca accttagcat
     5401 tcttagcgaa agtcttagga ttccccgtca aacggtcatc gaaacgagaa ccaatactaa
     5461 tcataaagtc cgcttccgtc atggcaatat ttgctgcgaa tgacccgtgc atgcctccca
     5521 ttccaagaaa gagtgggtga ctcgttgcaa tcgttccttg tcccaaaaga ctggttacca
     5581 ctggaatttg atagcgttct gcaaattcat ttagttccgt agcagcctca gcataactaa
     5641 ttccaccacc agctaacaag actggctttt tagccttgga caattgcttc aagattttct
     5701 tgatttgcat atcattcggc tcaacagtcg gctgataact tggtaaattc acttctggtg
     5761 aataaatgaa gtctgtttct aaagcagata tgtcttttgg taggtcaatt acaactggcc
     5821 ctggacggcc tgtagttgcg atatggacag cttccgtaat gatacgcgga atatcagctg
     5881 tctcacgaac ttggtaattg tacttagtga ttggcatggt aattcctacg atgtctgcct
     5941 cctgaaaggc atccttccca atccctgctc gcgccacctg acctgtaaag accaaaaggg
     6001 gaacgctatc gctcatagca tccgcaatcc ctgtaatggc atttgttgct cctggtccac
     6061 tagtgacgac ggcaacaccc aactttccag ttgatttggc ataaccttca gcttcatgca
     6121 aacaaccttg ctcatggcgc cctagaatgt ggcgaatgcc tttaaaatta tatatcgcat
     6181 cataaaaagg caaaaccgca ccaccaggat aaccaaagat ggtatcaact cctaaatcac
     6241 gaagtgtttc caaaactagg tccgaccccg tcttaggaga ttctaaactg attttctcca
     6301 ttgttcccct ttctttcctc ttaaaaataa cttgttacta tcataccatt tt
//
LOCUS       DAVLTM010000102         6319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLTM010000102 DAVLTM010000000
VERSION     DAVLTM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.103"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            96..260
                     /locus_tag="VYN16_001713"
     CDS             96..260
                     /locus_tag="VYN16_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1404215.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            344..910
                     /gene="amaP"
                     /locus_tag="VYN16_001714"
     CDS             344..910
                     /gene="amaP"
                     /locus_tag="VYN16_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1404216.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            922..1092
                     /locus_tag="VYN16_001715"
     CDS             922..1092
                     /locus_tag="VYN16_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1404217.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1131..1739
                     /locus_tag="VYN16_001716"
     CDS             1131..1739
                     /locus_tag="VYN16_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1404218.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1770..1973
                     /locus_tag="VYN16_001717"
     CDS             1770..1973
                     /locus_tag="VYN16_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1404219.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2083..2361)
                     /locus_tag="VYN16_001718"
                     /pseudo
     CDS             complement(2083..2361)
                     /locus_tag="VYN16_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2559..2846
                     /locus_tag="VYN16_001719"
     CDS             2559..2846
                     /locus_tag="VYN16_001719"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1404220.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3124..3894)
                     /locus_tag="VYN16_001720"
     CDS             complement(3124..3894)
                     /locus_tag="VYN16_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1404221.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3909..4757)
                     /locus_tag="VYN16_001721"
     CDS             complement(3909..4757)
                     /locus_tag="VYN16_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1404222.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYSHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4754..6230)
                     /locus_tag="VYN16_001722"
                     /pseudo
     CDS             complement(4754..6230)
                     /locus_tag="VYN16_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
BASE COUNT         2183 a         1188 c         1037 g         1911 t
ORIGIN      
        1 taaaataaaa aaggagggta ggcagtgttg tgatcaatta ttgctggagg tcttattggt
       61 ctcttggcag gtaaaatcac taaaaaagta gttctatggg aatcatcgca aatgtattcg
      121 ctggtttagt cggggcatat gcaggacaat ctcttttagg tagttggggt ccatcaatcg
      181 ctggaatggc tttgctccca tctattgtag gtgcagcgat tgtgattact gtagtgtcat
      241 tctttacagg tagaaagtaa acttttcgcc agtaaagtta gcaaactatt tttaaatcaa
      301 tgacgggaaa aatagtttaa atgttaaatc gaaaggattg tatatgtcaa aagcaaagaa
      361 aatatgtttc attattttct gtattttaat cttgacaatt ttccttcctg ttttgataga
      421 ttatcatcaa gttagtgatc taggtattca tctacttagc tggagacaga actccgtagt
      481 tgaattctat cttgctagat atgtcttttg ggggacagtg gttctatcaa ctttagtttt
      541 attatccatt ttagttgtga tgttttatcc taaacgttac ttggaaatcc aacttgaaac
      601 taaaaacgat acattaaaat taaagaattc ggcaatcgaa ggttttgtta gaagtttggt
      661 gagtgatcat agattgatca agaacccaac tgttcatgta aatttacgaa aaaataaatg
      721 tttcgttcat gtagaaggta aaattcttcc ttcagacaac atcgctgaca gatgccaaat
      781 aattcaaaat gaaataacta atggattgaa gcagtttttt ggtattgagc gtcaagtaaa
      841 acttgaagtt gcagtaaaaa attaccaacc aaaacctcaa aacaaaaaga ctgttagtcg
      901 tgtgaagtaa ggaagtaaaa aatggaatgg cttaaacaat atcgatatcc aattatcgct
      961 ggtctcatag gcgtatttct ggcttgtttg attgtctcct ttggcttctt caaaacaata
     1021 tttgtattga ttttaggagc actgggagtt gcagctggat tatatatcga aaaaaactat
     1081 atagataaat aaaaaaataa aaattactaa tttaattaaa ggagtttcat atgtcaaacg
     1141 aaaaaaacac aaacactaac gtagaaaaga aagatgctac tgttgtagct cacgaaatca
     1201 aaggggaact tacttacgaa gataaagtta tccaaaaaat cattggtctt tcactagaaa
     1261 acgtttcagg tcttttggga atcgatggtg gtttcttctc aaatcttaaa gaaaaaatcg
     1321 ttaacagcga tgacgtaaca agtggtgtta acgtagaagt tggtaaaaca caagttgcag
     1381 ttgacttaaa cgttattgtt gagtaccaaa aaaatgttcc agctttatat tcagaaatca
     1441 gagaaatcgt atcttcagaa gttgctaaaa tgactgactt ggaaattgtt gaaatcaacg
     1501 taaacgttgt cgacatcaaa actaaagaac agcatgaagc agactcagta agccttcaag
     1561 atcgcgtatc tgacgttgct gaatcaacag gagaattcac ttcagaacaa ttcgaaaaag
     1621 ctaaatctgg tcttggatct ggtttctcaa ctgttcaaga aaaagttagc gaaggtgtag
     1681 aagctgttaa aggtgcagca aatggtgtag tatctcacga aaacactcgt gtaaactaag
     1741 ataaaataaa tataacagga gaaattatca tgtcagtaga agaaaaatta aatcaagcta
     1801 aaggttctat taaagaaggt gttgggaaag ccatcggtga tgaaaaaatg gaaaaagaag
     1861 gagcagctga aaaagttgtt tctaaagtaa aagaagttgc cgaagacgct aaagacgctg
     1921 tagaaggtgc tgtagaaggt gttaaaaaca tgttgagtgg cgacgataaa taaggttaaa
     1981 agttacttta tctttttggt aacattagtc aaaagagtct gagtcaagat gattctcaga
     2041 aaacaaaaag ctagagattc ccaattgcgg aactctagct ttttaatttt gcctctttct
     2101 cttattatat ttcagcaggt tgttggccat gagtatgaat cccatgtcaa ttctcacttg
     2161 acgcttacct ctcagatgac atctcttata acccaaacaa acctttatct gcccaaagac
     2221 agatttcata tcaatcttac gtttagcgaa aatttgtcta cccttggaag ataaaagtgc
     2281 ctgatattct ttagttttta aacactggta acgttcattc atatacagtc tcttttgagg
     2341 ggctgattca ggttcataat cgcagttaac attgatttca aggctgtttg ctttctatct
     2401 cccctttttc atgatatatt ttcgtacagg ttcttcaata gtttctatag taaaatgaac
     2461 caaaaatagt acacgttgcg gtataatgtt cttgtggcat attcattaga ttttcgtaaa
     2521 cagttctctc ctatttgtga gcgaacaggt atagtcaatt gattgtcagt ttgaagacca
     2581 agagtcgtaa ggcaaaggat atggcagaat ccatccaagg gtggctggca caatttctag
     2641 tgaacctctt taaatctatt acgtttgact gtggaaaaga gttttcaaaa tggaaagata
     2701 tttccaatca tcacgatagt gagagctttt ttgcgaatct aggatgttca agacagcggt
     2761 gtctcaacga acattcaaat agacttttaa ggtgtcatga cttgccaaag cagacagatt
     2821 tcaacgaagt cagtcaagaa ttttaggctg gacaaaaact caatttcagg agaaaatgaa
     2881 ataaatattc ccacaataaa acgcatagta tcaaagtttt tccaacacct gatattatgc
     2941 attttttaat tttaaagact ttttcccagc ctcaagaaat cacatcccac ctaaatcctt
     3001 tggataccaa atacctcatc aggtattttc tgagttggta agactgtcta gcttaatttg
     3061 acaaatggag ggcgttaaat ccagttttta caggaagtct atttaaatat tttcacactg
     3121 accttacaaa atatgaaata aattatataa agtcattccc atgacaaaga ataacatccc
     3181 tttaaaacag tatcttaccc aattttcagg taagactttt gatacaaccg tccccaatac
     3241 ccctccgcat atagaagcaa gaaaaataac tggaacaggt gctaaatgat actgatttaa
     3301 acctgtaacc acaacaattg tagccaaatg agacagctgg gaaaagaaaa taattgcaag
     3361 agaatacatc gttgcttctt ttatcgaaat agaaaaaaac accatcaata aagatacatt
     3421 gagcggacca ccaccaatcc ctaaaaaact agaaattaag cccaatagaa gaccacaaat
     3481 catatagaat cctatcttat caaattgata agttttttta aaatctgtta aaacaaatga
     3541 tacaagtagc atcacaaata gtaaaatcat ttgaacaatg gatactagcc gtactgataa
     3601 ctgagataac aaaacttgaa aaataagatg acctagaaat cctcctaaaa cagaagaaaa
     3661 ggataccgta aaaatcaatc tccattttat ctctttactt tgtgaaaatc ttttgattgt
     3721 tgaaataatc gccattataa aaacagagaa actagaataa aaagcaatat cactgaccga
     3781 atggtatcca aaactatcca taagtggctt aattataatt ccaccaccca tgcctgatat
     3841 agaacccaat ccactagcta ttataatgat tagaaaataa ataatctcaa ccacttttta
     3901 agtccatcct attctttcaa acatctaaaa cctagatggc tactagtgga agtagaaata
     3961 catccatttc tagcagctac tcggtatcga ttacaataag aacaatgaca taaaaaggaa
     4021 cctcctctga tagcatactc ctcatcttgt atcccatatt ttggtaactc atagtcatct
     4081 tcattaaaac tagctaataa ggtatatctc ggattgcgac accattccca aacattccca
     4141 atcatttgat atagaccaaa atcattaggt aaaaattcat agacaggagc cgttcctata
     4201 aatccatcta aggcagtatt ttcataagga aatcttcctt gccaagtatt ggcatgataa
     4261 tatccacctt ctaaaagagt atctccccaa ggatattcag attgtcttcc tcctctagca
     4321 gcatattccc actgtgcttc agtcggtaaa gacattccgc tccaattaca gaaagccaat
     4381 gcatcttcta aagcaacatg aactactgga tggtcttcta aacccactag attactattc
     4441 tcaccgtagg gatgtttcca gcaagcgttc ggaacttgta accaccacgg agccccagca
     4501 ggatgagaat atccctccct ttctgcctct ggaacgaaca atataaaaac aaaagaccac
     4561 tcttgtcgct cagctagtgt tttatagcct gtttctttca caaattgagc aaattcttga
     4621 ttcgtaactg gagtatccgc aatggaaaaa tcctttacac taacagcgac ttgaggacct
     4681 tcaaaatcat ctaaaaagcc ctcttcagaa tttgttccca tcataaatgt cccacccttg
     4741 atttttttca ttttcatgat tgactatccc ccgttttcgt tagagttggc gttattttag
     4801 aaagttcaac aggaactaac tcttcatcca caacaaaacc ttcttctcta tatctaagaa
     4861 agtctactaa ttttttcttc atctgacgaa caataggttg gtatttttca cttggataca
     4921 aatcattcat ttcatgcgga tcttttttca tatcaaataa ctgataatga tttaaaacag
     4981 gaaaccagat aaatttccac tggtctgtca agatatattg actagaatct tttcctaaag
     5041 catgttcacc atgaaattct gttcgccacc cctcgtactg tccaaataat aaattcttaa
     5101 cactccgtcc atctagttca tctgtagtag ttcctcctgc caaatccacg agtgaaggaa
     5161 aaatatcttg aattttcact aattgcttga ttgttcccct attaccagct atcaatccag
     5221 ccggatcgta aataaacgaa ggaatgtgga tacttccctg ataaggatat ccctttctaa
     5281 acagataatg ttcaccaagc tgatctccat gatccgaaac aaaccagata atcgtatctt
     5341 tatcatgacg aaattctttt agggcagtca aaaagcgact aatttgatgg tctatatgag
     5401 taatcaatcc aaaataagca gcaaccattc ttctttgatc atcttccttt aatttccctc
     5461 ttaaagcata aatagacgga atttgctttt ctaatacttc ccaattcccg atatgcaaat
     5521 cgagaaattg aggcaagcgt tccatataca tatcaaaata gtattttggg ggatttaatg
     5581 gagcatgtgg tttttcaaaa gacattttga gaaaaaaagg aaccgttgga tctcttcttt
     5641 agtaaaaatg aaatggactc actcacaacc cagtttgttg gatgtaactt ctcatcttta
     5701 tcccaaggtc tagcttccca agaattacaa tccattccgt catcaatcaa atcaacatca
     5761 taaccaactt taccctttaa aaaggctaga tagtcactag cataatcaaa ttgacttcca
     5821 taagccttat catactttct atcaacatgt agatacccat cgtgaagtaa gacatgatca
     5881 aacccaagtc tctgtctgga aggaaaaaca tgcatcttac caatacactc tgtttgataa
     5941 cccatatctt taaagacttt tggaagagta ttagtaaaat tccaaggcac ctcatcttga
     6001 taaccaactc gtccactttt atcttggtct aagccagtca ataaagctgc cctagcagga
     6061 acacaagaag gaactggaga ataggcattt tcaaaattat aacctacact agccatcata
     6121 tctaatgttg gagtgctaac tagcttatcc ttactattca aggataagac gtctgctctc
     6181 atttgatcta caacaatcaa aataatattt ggctgttttg tctgaaccat aaaatctcct
     6241 ttctaatatg gcaaaagagg cacaagaaga tatctacctt tactgcaccc ctttctatat
     6301 caatctctct atataaaac
//
LOCUS       DAVLTM010000103         6284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLTM010000103 DAVLTM010000000
VERSION     DAVLTM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.104"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..1288)
                     /locus_tag="VYN16_001723"
     CDS             complement(<1..1288)
                     /locus_tag="VYN16_001723"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1404223.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="VYN16_001724"
     CDS             complement(1366..2613)
                     /locus_tag="VYN16_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1404224.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMLLPTLFTVFAGIRADYTRAKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3655)
                     /gene="rgg"
                     /locus_tag="VYN16_001725"
     CDS             complement(2804..3655)
                     /gene="rgg"
                     /locus_tag="VYN16_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1404225.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATRGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEELLFLARGFQYDTDSELRKEITDVLKPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVCIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3779..4120
                     /locus_tag="VYN16_001726"
     CDS             3779..4120
                     /locus_tag="VYN16_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1404226.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4178..4258
                     /locus_tag="VYN16_001727"
                     /pseudo
     CDS             4178..4258
                     /locus_tag="VYN16_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4373..6076)
                     /gene="ilvD"
                     /locus_tag="VYN16_001728"
     CDS             complement(4373..6076)
                     /gene="ilvD"
                     /locus_tag="VYN16_001728"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1404227.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT         1940 a         1378 c         1254 g         1712 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgagtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccacaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtactaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aagaatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaaataa gaaaccaaac
      421 catcgtaacg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgcctc cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatttggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatatagta gaacttgctt ggcttttgca cttctggggc gaagagttta ttttccacat
     1021 aggaacggac aacgggcgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttatag cgcttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ttcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccacaaa taaagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacaggg taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtcgcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgattg ctactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcaaat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaataaa ctagcttgct gaacaactag
     2341 ggctgccaga aaaaataaaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 ggccctcgtg taatctgccc gaatccctgc aaaaactgta aagagagtcg gaagaagcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccaga ttgaaaatcg aagcaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa ataaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caatagttct atcgcttcaa attttttctt ggtttgcaga tattcaacat tcggtcgtat
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacag acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcagatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctcttcata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttgatact
     3361 ccctgcgata taagtcctca agaggtgcta tattctttgg tttcaagaca tctgtaattt
     3421 cttttctcaa ctcagaatct gtatcatact ggaaacctct cgccagaaag aggagctcct
     3481 ccacactggc agatatattt tccagagcaa atagaaactt ttccaccgaa agctcactct
     3541 gacctgtttc aaaacgggac aacatagacg gcgaaaattg tcctctggtt gcttgtctca
     3601 gtgagatatt tcttgactct cgtaattgtc taaagacttt tccaatctgc tccatagact
     3661 tccccttgat tccgtatttt cttcatttta tcatattttt cagaaaattc atcaaaaact
     3721 tgccaaattg tcagaattat gagaaaatag aggatattta tcacgtggag ggactgctat
     3781 gagagacgat atcaaaatca atgaccgtgc tttggccttg caagaccaaa ttatcgaaaa
     3841 actagagaaa gtttttgata cagatgtgga attggatgtt tacaatctag gtctgattta
     3901 tgaaatcaat ctggatgaaa cggggctctg caagattgtc atgaccttca ccgatactgc
     3961 ctgtgattgc gccgaaagcc tgcctattga aatcgtggca ggtctgaaac aaatcgaggg
     4021 tatcaaagat atcaaggttg aagttacctg gtcgcctgct tggaaaatca cacgaatcag
     4081 tcgctatggc cgtattgccc ttggactacc acctcgttaa gcaggccaat cacttttaaa
     4141 gatgaaaaaa gcaagccgaa aatggcttgc tttttgatac tcaatgaaaa tcaaagggca
     4201 aactagaaaa ctagccgcag gttgctcaaa acactgtttt gaagttgtgg atagaactga
     4261 cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacgaag tcagtaaccg
     4321 tactacggca aggcgacgtt gacgtgattt gaagagattt tcgagtatga gtttattttt
     4381 tacctgactt gtccatattc cagaagtctg tcacggctcc gcgtgaagca gatgatacga
     4441 tgtgggcata tttaccgagg acaccacggc tgtaaagtgg tggcaaggtt gtttctgcct
     4501 tgcgtttttc aagttcttct tcggatacgg ccatggaaat ttctttggta tcttggtcaa
     4561 ccgtaacgat atcgccggta cggagatagg caattggtcc accatcctga gcttcaggag
     4621 cgatatgtcc aacaaccaga ccataagtac caccagagaa acgtccgtcc gtcaagaggg
     4681 ccaccttatc tccctgacct ttaccaacaa tcattgaaga aagtgacagc atctcaggca
     4741 taccaggacc acctttaggt ccaacaaaac gaacaacgac tacatcgcca tcaacgattt
     4801 catctgtcag aacggcctga atcgcatctt cttctgagtc aaagacctta gctggcccaa
     4861 cgtgacgacg cactttaaca cctgatacct tggcaactgc accgtcagga gcaaggttcc
     4921 cgttcaagat gataagcgga ccatccgcac gttttggatt ttcaagtggc atgataactt
     4981 tttggcctgg agtcaagtct gcaaagtcag ccaagttttc agctacagtc ttaccagtac
     5041 atgtgatgcg atctccgtga aggaaaccat ttgccaacaa gtacttcata accgcaggga
     5101 caccaccgac ttcgtagagg tcttggaaga catactgacc agatggtttc aagtcggcca
     5161 agtgaggcac acgttcttga atcgtattga agtcctcaag tgacaagtca acatttgcgg
     5221 catgggcaat ggcgagcaag tgaagagtgg cgtttgtaga accaccgaga gccatcgtta
     5281 cagtaatagc atcttcaaag gcttcacgag tcaagatatc tgatggtttg agaccaagtt
     5341 ccaacatctt aacaacagca cgtcctgctg cttcgatatc ttctttctta tcagctgatt
     5401 cagctgggtg agaggatgac cctggcaaac tcatccctag aacttcgata gcagttgcca
     5461 tggtattagc agtatacata ccaccacaac caccagggcc agggcaggca ttacattcaa
     5521 gacgtttcac gtcctcagct gtcatgtcac cgtggttcca ttttccgata ccttcaaaga
     5581 cagaaaccaa gtcgatatct ttaccatcaa gatttcccgg tgcaatagtt ccaccatagg
     5641 cgaaaatagc tgggatatcc atattagcaa tagcaatcat agatccaggc atgttcttgt
     5701 cacagccacc gatagcgacg aaggcatcca cgttgtgacc actcatagcc gcctcgatgg
     5761 agtccgcgat gatgtcacga gatgttagag agaaacgcat accaggcgtt cccatagcga
     5821 tcccgtccgc tacggtaatg gttccaaact gtacaggcca agcgcctgca gatttgacac
     5881 cttctttagc cagtttcccg aaatcatgca agtgaatgtt acatggtgta ttttccgccc
     5941 aagtcgaaat cactcccaca atcgatgttt caaagtcctt atctgtcata ccagtcgcac
     6001 gaagcatagc acggttaggt gatttaacca tgctgtcata gatgctactg cggtgacgtt
     6061 tatctaattc agtcatctta tccctcccat ttcagttttt actattatag cacaatttaa
     6121 aggcaatgaa cagaataaaa ttcttgaatt ttcagaaaat tctatacgca tgtgaaatat
     6181 ttaaaattaa aaacaacaaa gcggattagt gcactttctg atgaccagaa tatgcttttt
     6241 aatccgcttt ctttaaataa cgtaatgtaa tttttacaga aatt
//
LOCUS       DAVLTM010000104         6259 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLTM010000104 DAVLTM010000000
VERSION     DAVLTM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.105"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            244..927
                     /gene="rpiA"
                     /locus_tag="VYN16_001729"
     CDS             244..927
                     /gene="rpiA"
                     /locus_tag="VYN16_001729"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1404228.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKIVATPSKEYIWVVDESKLVDKLGAFKLPVEVVQYGAEQIFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIEDPIVFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QILTSRKEK"
     gene            941..2152
                     /locus_tag="VYN16_001730"
     CDS             941..2152
                     /locus_tag="VYN16_001730"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1404229.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            2154..2696
                     /locus_tag="VYN16_001731"
     CDS             2154..2696
                     /locus_tag="VYN16_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1404230.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            2712..3521
                     /locus_tag="VYN16_001732"
     CDS             2712..3521
                     /locus_tag="VYN16_001732"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1404231.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            4018..5625
                     /locus_tag="VYN16_001733"
     CDS             4018..5625
                     /locus_tag="VYN16_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1404232.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     TEPAQDSPILAPVEETKEEAVTEKPTNTRSLTAEDLVKISKGELHLENDLIDESFYGE
                     KALDLEGDDDQDGIKNKDGKDYLGYNSHPLLADSDGDGLADGEDDNKKEWYVTDRDSL
                     LFMELAYRDDDYIEKILYHKNLFPSLYLDRQEHKLMHNELAPFWKMKKAYYTDSGLDA
                     FLFETKSDLPYLKDGTVYMLAIRGTRVNDAKDLSADLVLLGGNKPAQADDIRKVVGEL
                     AKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDFYNHVLRKVTTFSAPKVITSRTVW
                     NAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKHRSRG
                     YFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPRSSSQPSAEPMENIAL
                     GKQVTQSSTVFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIRQINI
                     YNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            5650..6162
                     /locus_tag="VYN16_001734"
     CDS             5650..6162
                     /locus_tag="VYN16_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1404233.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         1945 a         1100 c         1443 g         1771 t
ORIGIN      
        1 attcttgaaa tgcgcctgct ttaaggctaa actagacgta tcaaagttaa gagtttgttt
       61 ttgtaactgg aaagagatga acaaatcaaa tacataaaag ataaactcat ttttattcgt
      121 ttggtgaaaa gaaaatacat atttttaaag aaagattgat gaaaaatgta gatttgatgt
      181 ttttacttga ataaaaagcg gtttcatagt aaaataagaa ctgagaatga tggaggtaaa
      241 tcggtggaaa atctgaagaa aatggcaggt atcaaggctg ctgagttcgt gagcgatgga
      301 atggtcgttg gactgggaac gggttctact gcctattatt ttgtcgaaga aattggtcgt
      361 cgtatcaagg aagaaggatt gcagattaca gctgtaacga cttctagtgt gaccagtaaa
      421 caggctgaag ggcttaacat tccactcaag tctattgacc aagtagactt tgtcgatgtg
      481 acagtcgacg gagcggatga agtagatagt cagtttaatg gaatcaaagg cggtggtggt
      541 gcccttctga tggagaagat tgttgcaacg ccctcaaaag aatacatttg ggtagtggat
      601 gaaagcaagc tggtagacaa actaggtgct tttaaattgc ctgtggaagt ggttcaatat
      661 ggtgcagaac agatttttcg tcgttttgag cgagctggct acaaaccaag tttccgtgaa
      721 aaagatggcc aacgttttgt gaccgatatg cagaacttta tcattgacct tgccttggat
      781 gtcatcgaag atccaattgt ttttggacaa gaattggacc atgtcgttgg tgttgtagaa
      841 cacggtttat tcaaccaaat ggtggacaag gtcattgttg ctggacgaga tggggttcag
      901 attttaacct caagaaaaga aaaatagaag ggggcataag atgtctaaat ttaatcgtat
      961 tcacttggtg gtattggatt ctgtaggaat tggtgctgca ccagatgcta ataactttgt
     1021 caatgcaggg gttccagatg gtgcctctga cacactggga cacatttcca aaaccgttgg
     1081 tttgaatgtc ccaaacatgg ctaaaatcgg tcttggaaat attcctcgcg aaacgccttt
     1141 gaagactgta gcagctgaaa gcaatccaac aggatatgcg acgaaattgg aagaagtatc
     1201 ccttggtaag gataccatga ctggtcactg ggaaatcatg ggactcaaca ttacggagcc
     1261 tttcgatact ttctggaacg gattcccaga agaaatcctg acaaaaatcg aagaattttc
     1321 aggacgtaag gtcattcgtg aagccaacaa accttactca ggaacagctg ttattgatga
     1381 ctttggacca cgtcagatgg aaactggaga gttgattatc tatacttcag ctgaccctgt
     1441 tttacaaatt gctgcccacg aagacatcat tcctttggat gaattgtacc gtatctgtga
     1501 atacgctcgt tcgatcactc ttgagcgtcc tgcccttctt ggtcgcatca ttgctcgccc
     1561 ttatgtaggt gaaccaggta acttcactcg tacggcaaac cgtcgtgact tggctgtatc
     1621 tccattttcc ccaactgttt tggataaatt gaatgaggct ggtatcgata cttatgctgt
     1681 gggtaaaatc aacgatatct ttaacggtgc tggtatcaac catgacatgg gtcacaacaa
     1741 gtcaaatagt catggaattg atacactatt gaagactatg ggacttgctg agtttgaaaa
     1801 aggattctca ttcacaaacc tagttgactt tgatgccctt tacggccatc gtcgtaatgc
     1861 tcacggttac cgtgattgct tgcatgagtt tgatgaacgc ttacctgaaa ttatcgcagc
     1921 tatgagagag aatgaccttc tcttgattac tgcggaccat ggaaatgacc caacgtatgc
     1981 aggaacggat cacactcggg aatatattcc attgttggcc tatagccctg cctttaaagg
     2041 aaatggtctc attccagtag gacattttgc agatatttca gcgactgttg ccgataactt
     2101 tggtgtggaa actgctatga ttggggaaag tttcttagat aaattggtat aagatgacgc
     2161 gctatgcttt gctggtgaga ggtatcaatg ttggtggtaa gaataaggtc gtcatggcgg
     2221 agcttcgtca agaattgaca aacttgggac tggaaaaggt tgagagctac atcaacagtg
     2281 gcaatatttt ctttacttcg atagattcca aagcccaatt ggttgaaaag ctagagactt
     2341 tctttgcagt ccattatcca tttattcaga gcttttcttt actgagtcta gaggactttg
     2401 aggcggaact tgaaaatcta ccagcttggt ggagcagaga cttggcacga aaagattttc
     2461 tcttttacac tgagggtttg gatgtggacc aagtcatcgc gacagttgaa agtttagagc
     2521 tgaaagatga agtgctttat tttggaaaac ttgggatttt ctgggggaaa ttttctgaag
     2581 aatcctattc taagactgcc tatcagaagt acttgctgaa ggtgcctttc taccgccaca
     2641 ttactattcg taatgctaaa acctttgaca aaattggtca aatgctaaaa aaataataaa
     2701 ggagacacac aatgacattt ttaaacaaaa tccatgaaac tgctactttc ctgaaagaaa
     2761 agggaattgc agcccctgag ttcggtctaa tccttggatc aggacttgga gaattggcag
     2821 aagaaatcga aaatccagtt gtagtagact atgctgagat tccaaactgg ggccgttcaa
     2881 cagtagtcgg tcatgctggt aaattggtat atggtgaact ggcaggtcgc aaggtcttgg
     2941 ctcttcaagg gcgtttccat ttctatgaag ggaatcctct ggaagtggtg actttcccag
     3001 ttcgtgtgat gaaagttctt ggatgtgaag gtgttattgt aaccaatgca gctggcggta
     3061 tcggatttgg tcctggtacc ttgatggcta tctcagacca tatcaacatg acggggcaaa
     3121 atccattgat gggtgaaaac ttggatgact ttggcccacg tttcccagat atgtctaggg
     3181 cctacacacc agaataccgt gccactgccc atgaagtggc taaaaaactt aatatcaagc
     3241 ttgatgaagg tgtctatatc ggagttactg gtccgactta tgaaacacca gcagaaattc
     3301 gttcctataa gacactggga gcagatgcag ttggtatgtc tacggttcct gaagttatcg
     3361 tggcagccca ctctggcttg aaagttctgg gaatttcatg tatcactaac tttgcggccg
     3421 gtttccaaga agaactcaat cacgaagaag ttgtagaagt gactgaacgt gttaaaggtg
     3481 atttcaaagg cttgcttaaa gcgattcttg ctgaattgta agaaaaaaga tttaaaaggg
     3541 gaagtgcctc tgttttttca ggattgactg cctatccgga ttaaagaaga aacagaggaa
     3601 tactatgagc ttcttcctgc tcttataact gaaagaagcg gaagaatagg tatgtctgat
     3661 ctgatagcca gcattgtgaa agacaagatt ctaggatact agcattagct tcctagccaa
     3721 gcagactagt atgataagga gagatgagaa tgaattgact ttctgaattt ctcagtctta
     3781 tcatatatag cacaatgaga tttcgcttga gtctgcttgt aaataaacga aaagaaagat
     3841 aagaaataat gaaaattggt caacgaatta tgcgctttgg cataaaaaat taagtatcgg
     3901 agttgtatct gttgtagtcg gctttgattt ctagctccag ctggaatttc agccaatgaa
     3961 gtaaagcaag atgtaacatc tgaagtggta ataggtgtgc tagattctaa ggaggaattg
     4021 aaagagtcag aaaatgatgc tccaaaacta gaaactcctc ttagagagga gccaagacta
     4081 gctcctcaaa cgcttccgga agcaagtgaa gttcttgaaa acaaaaggga agagtcaaaa
     4141 gtagagataa cagagccagc tcaagactca cctattcttg ctcctgtaga agaaactaaa
     4201 gaagaagctg tgacagaaaa accaacaaat actcgttctc taactgcaga agatttggtg
     4261 aagatttcca aaggggaatt gcatttagaa aatgatttga ttgatgaatc tttctatggt
     4321 gaaaaagctc ttgatttgga aggggatgat gaccaggatg gcatcaaaaa caaagatggt
     4381 aaggattatc taggatataa cagtcatccc ttgctagcag acagtgatgg ggatggtttg
     4441 gcagatgggg aagatgataa taagaaagaa tggtatgtca cagaccgtga ttctcttctc
     4501 tttatggagt tagcttatcg agacgatgat tatattgaga aaattttata tcataagaat
     4561 cttttcccta gtctctatct tgaccgtcaa gaacacaaac tcatgcacaa tgaattggct
     4621 cctttctgga agatgaaaaa agcctactat acagatagtg gcttggatgc tttcttattt
     4681 gagaccaaga gcgaccttcc ttatctcaaa gatggaacgg tgtacatgtt ggctattcgt
     4741 ggaacgcgag ttaatgacgc caaggacttg agtgcagatt tggttttatt aggtggaaat
     4801 aaaccagctc aagcggatga tatccgcaag gttgttgggg aattagccaa ggatataagt
     4861 attactaagt tgtatatgac aggtcattct cttggatgtt acctagctca gattgcagcg
     4921 gttgaagctt accaaaaata tcctgatttt tataaccatg tattgaggaa agtgacaact
     4981 ttcagtgctc ctaaagtgat tacttccaga actgtttgga atgctaagaa tggtttctgg
     5041 gatgttggtt tggaaagtcg taaattagct gttagtggaa aaattaagca ttatgtggtt
     5101 gataatgaca atgttgtgac tcccttgatt cataataatc gtgatattgt tacatttaca
     5161 ggtaattcac gctttaaaca ccgttctcgt ggctattttg aaagtccaat gaatgatatt
     5221 cctaacttta atattggtaa acaagctacc ttggataaac atggttatcg tgatccgaaa
     5281 ttggataaag tgcgattctt taagaaacag gctctgcctc gatcttctag tcaaccaagc
     5341 gctgaaccaa tggaaaatat tgccttagga aaacaggtta ctcaaagttc gacagttttc
     5401 ggaggagatg ctagaagagc tgtggatggc aaagtcgatg gtaactatgg tcacaattct
     5461 gtcactcata caaacttcca atctaagcct tggtggcaag tagatttggc taaagaagaa
     5521 accattcgcc aaatcaatat ttacaaccga acagacactg cccaggatag attggcaaac
     5581 tttgatgtca ttcttttaga cagttctggt aaagaaattg agtaaaaacg tataacatct
     5641 cctaaagatg tgtcagcaca aattacgatt aaccataaaa aagtacgcta tgttcggatt
     5701 gagctagaag gctataatgc cctcagtctt gcagaagttg aagttttccg ctttatagct
     5761 acgaatgctg aaacggcgac acaagtttct aagccagttc aaccaatcag tcagactcct
     5821 gtgaaagata aaacattgac aattcaacac agtggagctt acattgcccg ctactccata
     5881 acttgggaag aagttccagt agataaagat ggaaaccaag ttgttcgtag tcattcttgg
     5941 gaaggaaacg gttgcaacca gactgcaggt tttgtcctca acctcccaat caaagaaaat
     6001 atgagaaatc tgcgagttaa gattgagaaa aagacgggcc tactatggaa tagatggcaa
     6061 acaatctatg aaaacagacc aattttagct caaccccacc gtaaaattac ccattggggt
     6121 acgacattga attccaaggt gagtgacgat gatgtcttgt aatctgatgg tacaatgaca
     6181 gttagtttgt ctagtttata agaaagtact acctgagctt gaatagaact caggtagctc
     6241 tctatgaaag aacaaaatt
//
LOCUS       DAVLTM010000105         5986 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLTM010000105 DAVLTM010000000
VERSION     DAVLTM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.106"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            29..>5986
                     /locus_tag="VYN16_001735"
     CDS             29..>5986
                     /locus_tag="VYN16_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1404234.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     EPAVQPELPEAVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSPDTSSENTGEVSNKPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTKSNTSNSNGNEEIKQENELDPDKKVEDPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVELSTDD
                     KSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFENLSGATVEKLSLKNVAISGKNDIGSL
                     ANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESSFKGRIVNTYETTDSYNI
                     GGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYTGMKVANTFS
                     SKANRVFNVTLEKNEVVSKESFEERGTMLDASQIASKKAEINLITPPIVEPLSTSGKK
                     DSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVKENSILYQKELLSAVMMK
                     DDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQADFANLAEYSIGDSGLLYTPNQF
                     LYHQDSIINQVLPELNRVNYQSDAVRNTLGISPEVKLTELYLEEQFTKTKEHLAENLK
                     KLLSSDAGLVTDNEVMTGYIIDKIKRNKEALLLGMSYLERWYNFSYGQVNVKDLVMYH
                     PDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYAISLASHHGTTDLFSTLENYRKV
                     FLPDKTNNDWFKSQTKAYIVEEKSNIEEVKTKQGLVGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTISSLGFDAYDRYRNKEHQANGDLNSFVEKSAHETAERQRDH
                     YDYWYRILDEKGREKLYRNILLYDAYKFGTNHTEGKATEVADFDSPNPAMKHFFGPVG
                     NKVGHNGHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHNSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDQPSDATITINSILKHSKSDSKEGERLQVLDPTTRFKDATDLQKYVH
                     NMFDVVYMLEYLEGKSIVKKLNVYQKIEALRKIENQYLTDPADGNDVYATNVVKNLTE
                     DEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSGEKGTPGDLMG
                     RRIAFELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGKERGLVTDELVLKKVFDG
                     KYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPRYGTKTINNVDELQKLMDEAVL
                     QDAKERNYYYWNNYNPETDSAVHKLKRA"
BASE COUNT         2219 a         1010 c         1205 g         1552 t
ORIGIN      
        1 tatataaatt ttatttcaag gaggaataat ggaaaagtat tttggtgaaa aacaagagcg
       61 tttttcattt agaaaattat cagtaggact tgtatctgca acgatttcaa gtttattttt
      121 tatgtctgta ttagctagtt catctgtgga tgctcaagaa actgcgggag ttcactataa
      181 atatgtggca gattcagagc tatcatcaga agaaaagaag cagcttgtct atgatattcc
      241 gacatacgtg gagaatgatg atgaaactta ttatcttgtt tataagttaa attctcaaaa
      301 tcaactggcg gaattaccaa atactggaag caagaatgag aggcaagccc tagttgctgg
      361 tgctagctta gctgctctgg gaattttaat ttttgctgtt tccaagaaaa aggttaagaa
      421 taaaacggta ttacatttag tattggttgc gggaatagga aatggtgtct tagtttcagt
      481 ccatgcttta gaaaatcatc ttttgctaaa ttacaatacg gactatgaat tgacctctgg
      541 agaaaaatta cctcttccta aagagatttc aggttacact tatattggat atatcaaaga
      601 gggaaaaacg acttctgatt ttgaagtaag taatcaagaa aaatcagcag ccactcctac
      661 aaaacaacaa aaggtggatt ataatgttac accaaatttt gtagaccatc catcaacagt
      721 acaagctatt caggaacaaa cacctgtttc ttcaactaag ccgacagaag ttcaagtagt
      781 tgaaaaacct ttctctactg aattaatcaa tccaagaaaa gaagagaaac aatcttcaga
      841 ttctcaagaa caattagccg aacataagaa tctagaaacg aagaaagagg agaagatttc
      901 tccaaaagaa aagactgggg taaatacatt aaatccacag gatgaagttt tatcaggtca
      961 attgaacaaa cctgaactct tatatcgtga ggaaactata gagacaaaaa tagattttca
     1021 agaagaaatt caagaaaatc ctgatttagc tgaaggaact gtaagagtaa aacaagaagg
     1081 taaattaggt aagaaagttg aaatcgtcag aatattctct gtaaacaagg aagaagtttc
     1141 gcgagaaatt gtttcaactt caacgactgc gcctagtcca agaatagtcg aaaaaggtac
     1201 taaaaaaact caagttataa aggaacaacc tgagactggt gtagaacata aggacgtaca
     1261 gtctggagct attgttgaac ccgcaattca gcctgagttg cccgaagctg tagtaagtga
     1321 caaaggcgaa ccagaagttc aacctacatt acccgaagca gttgtgaccg acaaaggtga
     1381 gcccgctgtc caacctgaat tacccgaagc agttgtaagt gataaaggtg aaccagagca
     1441 ggtagcacca ttgccagaat ataaggggaa tattgagcaa gtaaaacctg aaactccggt
     1501 tgagaagacc aaagaacagg gtccagaaaa aacggaagaa gttccagtaa aaccaacaga
     1561 agaaacacca gtaaatccaa atgaaggtac tacagaagga acctcaattc aaggagcaga
     1621 aaatccagtt caacctgcag aagaatcaac aacgaattca gagaaagtat caccagatac
     1681 atctagcgaa aatactgggg aagtatccaa taaacctagt gattcgacaa cctcagttgg
     1741 agaatcaaat aaaccagaac ataatgactc taaaaatgaa aattcagaaa aaactgtaga
     1801 agaagttccg gtaaatccaa atgaaggcac agtagaaggg acctcaaatc aagaaacaga
     1861 aaaaccagtt caacctgcag aagaaacaca aacaaactct gggaaaatag ctaacgaaaa
     1921 tactggagaa gtatccaata aacctagtga ttcaaaacca ccagttgaag aatcaaatca
     1981 accagaaaaa aacggaactg caacaaaacc agaaaattca ggtaatacaa catcagagaa
     2041 tggacaaaca gaaccagaac catcaaacgg aaattcaact gaggatgttt caaccaaatc
     2101 aaacacatcc aattcaaatg gaaacgaaga aattaaacaa gaaaatgaac tagaccccga
     2161 taaaaaggta gaagatccag agaaaacact tgaattaaga aatgtttccg acctagagtt
     2221 atacagtttg tcaaatggta cttataaaca acacatttcg ttagagcaag ttccaagcaa
     2281 tccaaatagc tactttgtta aagtgaaatc ttcttcattc aaagatgtat acctaccagt
     2341 agcatcaata tcagaggaaa gaaaaaatga taaaatcctt tataaaatca cagcaaaagt
     2401 agagaagctt cagcaggaga tagaaagcag atataaagat aattttacct tctatctagc
     2461 taagaaggga acagaagaaa caacaaactt tacttccttt agtaatctgg tcaaagctat
     2521 aaaccaaaat ctctctggaa cctatcattt aggagctagc ctgaatgcta acgaagtgga
     2581 gcttagtact gatgataaat cctatatcaa gggaaccttt actggtcagt tgattggtga
     2641 aaaagatggt aagcattatg ctatttacaa cttgaaaaag cctctttttg aaaacttgag
     2701 tggtgctaca gtagaaaaac tgagtctaaa aaatgttgct atttcaggga aaaatgatat
     2761 tggttcactg gcaaatgaag ctacgaatgg cacaaagatt aaacaagttc atgttgatgg
     2821 tgttcttgct ggagaacgtg gtgtcggtgg tttgttggct aaggcagacc aatcaagcat
     2881 cgcagagagc agtttcaagg gaagaattgt caatacctat gaaacgactg attcctacaa
     2941 tattggcggt ctggtcggtc atttaacagg aaaaaatgcg tctattgcta aatccaaagc
     3001 gacagtaacc atttcgtcaa acaccaatag gtcagatcag actgtcggtg gtcttgcagg
     3061 tctagtagac cgagatgcac agatccaaga tagctatgct gaaggtgata tcaacaatgt
     3121 caagcacttt ggtagagtcg ctggagtggc aggcaatttg tgggatcgaa cttctggtga
     3181 tgttaggcat gctggaagtt tgaccaatgt tctcagcgat gttaatgtaa caaacggaaa
     3241 tgccatcact ggataccact atacaggaat gaaggtagct aatacattta gtagtaaggc
     3301 taatagagtt ttcaatgtca ctttagagaa gaatgaggtc gtcagcaaag aatccttcga
     3361 ggaaagagga acaatgctag atgcttctca aattgcaagc aaaaaagcag aaatcaatct
     3421 tatcactcca ccaatagtgg aacccctctc aacaagcggt aagaaagaca gtgatttttc
     3481 taagatagcc cattatcaag ccaaccgtgc tttggtttat aagaacattg aaaaattgtt
     3541 acctttctat aacaaggcga ccatcgtgaa atacggaaac ctggttaagg agaacagtat
     3601 cttatatcaa aaagaactct tgtctgcagt tatgatgaag gatgaccaag taatcacaga
     3661 tattatttcc aacaaacaga ctgcaaacaa actcttgctt cactacaagg accattcatc
     3721 tgaaaagttt gatctcaggt accaggctga ttttgccaat ttggcagaat atagcattgg
     3781 agatagtggg cttctctata cgccaaatca attcttgtat catcaagatt ctattattaa
     3841 ccaagtgctg cctgaattga atcgagtaaa ttatcaatca gatgctgtta gaaatacact
     3901 tggaatttca ccagaagtga agctaacgga gttatatctt gaagaacaat ttaccaaaac
     3961 gaaagagcat ctagcagaga acttgaaaaa acttttatca tctgatgcag gtttagtaac
     4021 agacaatgaa gttatgacag gatatataat agataaaatt aaacggaata aggaagcctt
     4081 acttcttgga atgagttatt tagaacgttg gtataacttt agctatggtc aagtgaatgt
     4141 caaagaccta gttatgtatc atccggactt ctttggtaaa ggaaatactt caccattaga
     4201 tactctgatt gagttaggta aatctggctt taacaatctt cttgctaaaa acaatgtcga
     4261 tacttatgct atcagccttg ccagccatca tggaacgaca gatttgttta gcacgttgga
     4321 aaattaccga aaagtctttc taccagataa aaccaataat gactggttta aatcacagac
     4381 taaggcttac attgtcgaag aaaaatccaa tatcgaagag gtgaaaacga agcaaggact
     4441 agttggcact aagtattcta ttggtgtcta tgaccgtatc actagtgcca catggaaata
     4501 ccgtaatatg gtactacctc tacttacctt gccagaaaga tccgtatttg tcatttcaac
     4561 catatctagt ctaggttttg atgcttatga tcgctatcgt aataaagaac atcaagcaaa
     4621 tggtgatctt aatagctttg ttgaaaagag tgctcacgaa acagcagagc gtcaacgaga
     4681 tcactatgat tattggtatc gtattctaga tgaaaaaggg cgtgaaaagc tttatcgaaa
     4741 tatcttgctt tatgatgcct ataaatttgg aactaatcat actgaaggaa aggctacaga
     4801 agtagcagat tttgatagtc caaatcctgc aatgaaacat ttctttggac ctgttggaaa
     4861 taaggttgga cataatggac atggtgctta tgctacaggg gatgcagtat attatatggg
     4921 gtatcgcatg ctagataagg atggagctat tacgtatact catgagatga cgcataattc
     4981 agatcaagat atttatcttg gtggttatgg aagaagaagt ggacttggac ctgagttctt
     5041 tgctaaaggc ttactacaag caccagacca accaagtgat gcaacgatta ctatcaattc
     5101 tatattgaaa cattcaaaat ctgatagtaa agaaggagaa cgattacaag tacttgatcc
     5161 aactacgaga tttaaagacg caacagatct tcaaaaatac gtccacaata tgtttgatgt
     5221 tgtttatatg ttggaatatc tcgaaggtaa atctattgtt aagaaattaa atgtatatca
     5281 gaaaatagaa gctctcagaa aaatcgagaa ccaatatcta acagatcctg ctgatggaaa
     5341 tgatgtttat gctactaatg ttgtgaaaaa tttgactgaa gatgaagcta aaaaattgac
     5401 gagctttgat agtttgattg acaataacat tctttcggct cgtgagtata aggctggcac
     5461 ttatgagaga aatggctact ttactataaa actctttgca ccaatctttt cagcattgag
     5521 cggtgagaaa ggaactccag gtgaccttat gggacgaagg atagcttttg aacttctagc
     5581 tgctaaagga tttaaagatg gtatggtacc atatatttcc aaccaatacg aagaagatgc
     5641 taaacaacaa gggcaaacta tcaatcttta tggtaaagaa cggggcttgg tgaccgatga
     5701 gcttgttttg aaaaaggtat ttgatggtaa gtataaaact tgggcagaat ttaagacagc
     5761 tatgtaccaa gaacgggtgg atcagtttgg aaacttgaag caggtgacct ttaaagatcc
     5821 gacaaaacca tggccaagat atggcacaaa gactatcaat aatgtggatg aattgcagaa
     5881 actaatggat gaagctgttt tgcaggatgc aaaagaacgt aattattatt attggaataa
     5941 ctataatcca gaaacggaca gtgccgtcca taagttgaag agagca
//
LOCUS       DAVLTM010000106         5968 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLTM010000106 DAVLTM010000000
VERSION     DAVLTM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.107"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..792
                     /locus_tag="VYN16_001736"
     CDS             1..792
                     /locus_tag="VYN16_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404235.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     IGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTKPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILISSHKL
                     EDIEEICERVLFLESGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            808..1536
                     /locus_tag="VYN16_001737"
     CDS             808..1536
                     /locus_tag="VYN16_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404236.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     ETSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            1921..2130
                     /locus_tag="VYN16_001738"
     CDS             1921..2130
                     /locus_tag="VYN16_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1404237.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2120..2608
                     /locus_tag="VYN16_001739"
     CDS             2120..2608
                     /locus_tag="VYN16_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404238.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2724..4184
                     /locus_tag="VYN16_001740"
     CDS             2724..4184
                     /locus_tag="VYN16_001740"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1404239.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEGLRFSDSDIAYLELLGYHGAFLNYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4181..5005
                     /gene="nadE"
                     /locus_tag="VYN16_001741"
     CDS             4181..5005
                     /gene="nadE"
                     /locus_tag="VYN16_001741"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1404240.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRSETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5403..5954
                     /locus_tag="VYN16_001742"
     CDS             5403..5954
                     /locus_tag="VYN16_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404241.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1841 a         1018 c         1284 g         1825 t
ORIGIN      
        1 ataaattttg cacttaacaa gggtgaaatt gttggtctag tagggagaaa tggagttggt
       61 aagagtacgt tgatgaaaat tcttgttcag aataatcaac cgacttcagg taatattata
      121 agcagtgata atattgggta tttaatcgaa gaaccaaaat tatttttatc taaaacaggt
      181 ttagagaatt taaaatattt gtcaaattta tatggtgttg actacaatca agaaagattt
      241 agaagtttga tccaagagtt agatttgact cagtctatta ataaaaaagt aaagacctat
      301 tctttgggta caaaacaaaa attagctttg cttctaactc tcgttacgaa acctgatata
      361 ttgattttag atgaaccgac taatggttta gatattgaat catcacaaat agttttagcg
      421 gttctaaaaa aattagcttt aaatgaaaac gtgggaattt taatatcgag tcataaatta
      481 gaagacattg aagaaatttg tgagagggtt cttttcttgg agagcgggct tttgacattt
      541 caaaaagtag gaaaagatag tcataatttc ttgtttgaga tagctttttc atcagctaca
      601 gatagagaca ttttcattac caaacaagaa tttggggata ttgttcagga agagggactg
      661 agaattacta tgtctgggaa tattcaaagt agtgagcttt ttaaattttt taacgaaaac
      721 tctattaaag tagttgattt tgaaactaaa aaagagacgc ttaaagatat ttacttaaat
      781 cgttcaaaat aaaggaaggt tataatcatg aaattaaata aattgaattt tcttaaggaa
      841 aatataagaa atttatattc atcaggcgta atatatctca gtttgcttat ctcgtttata
      901 ccgccgatat tgcttacatt ctttattcta aagactcaag agacatcgct tggtattaag
      961 catatttcaa acttttatgc tatgctcggt atgttaatgg ctgttataca tgctaatcga
     1021 gttattagta gagatttttc ccacaatacg gtaagtttgt tttataatca acagaagaat
     1081 cggatgattt acgtcttgtc taattttcta tatgccatct cagtttccat tatttatgct
     1141 ttgaatggca ttgtgttact agtcatagta agtaaattgg gtattccagg tgatttagga
     1201 ttagatttta tagtagctat tgtagtcaat acaattttgt tagtcctgtt ttattttcta
     1261 ttatcttaca ttttctattt atacaaattg aaaagtggct tggtatttgg tattttagta
     1321 gctttactac tctttatccc taatatatta aatacgatga tgatgaatac tagtaatgat
     1381 ttgtttatca aagcaattga acttcttcct ttttattctt tacctgtatt tgtggcttca
     1441 aatacgatgt ctattagtca gtatcttgtg gtaatcacta caatcatttt attgtacttt
     1501 ttcactctca agaaaagcaa gaagtattca ttttagtttc atttagtatt attttgcata
     1561 cctaaaatac agtaaaaaat cagtcatctt ggtatgctcc tgctttcact attcaacacg
     1621 tttttgactt atactaggct catttccaaa agcattatat aatagtgata tgaaaccaac
     1681 taaactaaac aagaaatata agcaataaaa attcgtttaa aagatcttac taaagctaat
     1741 actaaataaa aataaaagag taaactagga agtttatttc aaacaaccta aaatactgat
     1801 tttcggctga agataatact ggaatgcaaa ttaatggggt tataataaat agctgatagc
     1861 ttgtgttggt tttggatttt ttaagagtag atgagtatta aaactataag gaggacgaag
     1921 gtggctaaaa atttaaaatt aaaattagct cgggtagagc gtgatttaac acaaggtcaa
     1981 ctggcagagg ctgtcggggt gacacgccag actattggtt taatagaggc gggaaaatac
     2041 aatcccagtc tctcgctctg ccagtctatt tgcagatgtt tagggaaaac cctagaccaa
     2101 ctattttggg aggaagaaga tgaaaaatag attttattat tatcaattac tagacgaaag
     2161 agaagaacaa ctgttcaata aagcgggctc tgaaagtttc tatatctgca ttgctttgtc
     2221 gctcctatct tatatcattt cagtattagc accaagcctt tttaattcta atatgctgct
     2281 aatcgttatc atcataggga cattttactt tttcaatcgt gcccgttatc tgggagtgac
     2341 ctactatagt cgttttcatt ttacgatttt gggttgtttt ttcctaacct tggctattac
     2401 ggctcttttg atgttgcaga attatcaatt caacatagaa atttatcagc acaatccttt
     2461 gaattttaaa tacctgtctg cttgggtcat tacttatatc atttaccttc cgtggatctt
     2521 tattggcaat cttggtctta agagctatgg cgaatgggct cagaaaaaat ttgaacaaga
     2581 tatggatgaa ttggagagtg gagaatagct tgttactctt ttctcaatcc agctaaaatg
     2641 tgatataata gtactaattt attggaatac atgaaagttc ttgaaaattt tcatgggttt
     2701 ctagctaagg aagtaggaaa agtatgtatc cagatgatag tttgacattg cacacggact
     2761 tgtaccagat caacatgatg caggtttact ttgaccaagg gattcacaat aagaaggcgg
     2821 tctttgaggt gtatttccgc caacagcctt ttaagaacgg ctatgcggtt tttgcaggtt
     2881 tggaaagaat tgtgaactat cttgaaggct tgcgtttttc agatagtgat atagcctatt
     2941 tggagttgct tggttatcat ggggcgttct tgaattacct tcgcaatttc aagttggagt
     3001 tgaccgttcg ttctgcccaa gaaggggatt tggtttttgc taatgaaccg attgtgcagg
     3061 tggaaggacc tctagcccaa tgtcagttgg tcgaaacggc tcttttgaac atcgtcaact
     3121 accagacctt ggtggcgacg aaggcagctc gtattcgttc ggttatcgaa gatgaaccct
     3181 tgatggagtt tgggacacgt cgggctcaag aaatggatgc ggccatctgg ggaacacgcg
     3241 cagcggtgat tggtggcgcc aatggaacca gcaacgtgcg tgcgggtaag ctctttgaca
     3301 ttcctgtttt gggaacccat gcccatgcct tggtacaggt ttatggcaat gactatgaag
     3361 ctttcaaggc ttacgctgcg acccacaaaa attgtgtctt tcttgtggat acctatgaca
     3421 cccttcgcat cggtgtacca gctgccattc aggtggcgcg tgagctgggt gatcagatta
     3481 actttatggg tgtgcggatt gactctgggg atattgccta catttctaag aaagtccgtc
     3541 aacaactgga cgaggctgga tttacagagg ctaagattta tgcttctaat gatttggacg
     3601 aaaatactat cctcaacctc aagatgcaaa aggccaagat tgatgtctgg ggcgtgggta
     3661 ccaagctgat tacagcctat gaccagccag ctcttggtgc agtttacaag attgttgcaa
     3721 tcgaagatga aactggtcag atgcgcaata cgattaagct gtctaataat gcggaaaaag
     3781 tctctacgcc aggtaagaag caggtgtggc gcattaccag tcgtgaaaaa ggcaagtcag
     3841 aaggcgacta tatcacttat gatggtgtgg atattagcga catgacagaa atcaagatgt
     3901 tccatccgac ctatacatac atcaagaaga cggttcgtaa ttttgatgcc gttcctctct
     3961 tggtggatat cttcaaagaa ggaatattag tttacaactt gcctagtttg actgacattc
     4021 aggattatgc ccgtaaggaa tttgacaagt tgtgggatga gtataagcgt gtgctcaatc
     4081 cgcagcacta tccagtggat ttggctcgtg atgtatggca agataagatg gacttgattg
     4141 ataagatgcg caaggaagcc cttggtgaag gagaagaaga atgagtttgc aagaaacgat
     4201 tatccaagag ctgggtgtca aaccagtgat tgatgcccag gaagaaatcc gtcgttctat
     4261 tgatttctta aaaagatatc tgaaaaaaca tcccttccta aaaacctttg tactagggat
     4321 ttctggggga caagactcaa ccttggcagg gcgtttggcg caattagcta tggaagaact
     4381 gcgatctgaa acgggagacg acagctacaa atttatcgct gtccgcctgc catacggagt
     4441 gcaagctgat gaagcagatg ctcaaaaagc tctagccttc atccagccag atgtcagctt
     4501 ggttgtgaat atcaaggaat cagctgatgc catgacagct gcagttgaag cgacaggtag
     4561 tcctgtttca gacttcaaca aggggaatat caaggcacgt tgccgtatga ttgctcagta
     4621 tgcccttgct ggttcccata gcggagcggt cattggaaca gaccacgccg cggaaaatat
     4681 cacaggtttc tttaccaagt ttggtgacgg cggtgcggat attctccctc tttaccgcct
     4741 caataaacgc caaggaaaac agctcttgca gaaacttggc gcagagccag ccctttatga
     4801 aaaaatccca acggcagacc tagaagaaga taaaccaggc ctagctgacg aagtcgcact
     4861 tggagtcacc tacgcagaga ttgacgacta cctagaaggc aaaacaatca gcccagaagc
     4921 tcaagcgacc attgaaaact ggtggcacaa aggccaacac aaacgccact tacccatcac
     4981 tgtatttgat gacttttggg agtaaaaacc tccattggag gtttttaccc tgagcctaga
     5041 aatgagaaag cgaagaaggt ccgggggact ttttagcttc ttgccctgaa attaaaaagc
     5101 aagaaaaacc tccactggag gttttcagcc tctcatcttg aaataagaaa gtgagagaag
     5161 gtctggggga tcttgaaccc cgagtttaga aataagaaaa tgaggcagat tcagtaactc
     5221 gaagagttcg atttcatcgt cttacccctg caacgatgac taggtttgaa aaagcttgct
     5281 agagcgcatt tcaaaccagg cagcaactgc gtcaagaaat tagaagacaa actcgttttc
     5341 tagctgttac tgagttgagc ctttttacta cgagtataga aataaggaag tgaggtagca
     5401 tcatgaaatc tatcggtacg caaatattac agacagaacg tttgatttta agaagatttg
     5461 tggagagtga tgcagaagcc atgtttcaaa attgggcttc atccgctgag aatctgacct
     5521 atgttacctg ggatccccat cctgatgtcg aaatcactcg aaactcgatt tgcaattggg
     5581 ttgcttccta tactaatctc aactattata aatgggccat ttgtctaaaa gaaaacccag
     5641 agcaagtgat aggagatatc agcattgtta agatagacga ggctgattta agctgtgaaa
     5701 ttggctatgt gttaggcaag gcttactggg gaaatggtat gatgacagag actttgaaag
     5761 ctatcttgga cttttgtttt actcaagcag gttttcaaaa ggtcagagca cgttatgcca
     5821 gtctcaaccc agcttcaggt cgtgtcatgg aaaaggctgg aatgtcctat ctacaaacca
     5881 ttgttaatgg tgtagagaga aaaggctatc ttgcggatct tatttattat ggtataagta
     5941 gggaagaatg ttgaattcta ttttctgt
//
LOCUS       DAVLTM010000107         5919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLTM010000107 DAVLTM010000000
VERSION     DAVLTM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.108"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            74..390
                     /locus_tag="VYN16_001743"
                     /pseudo
     CDS             74..390
                     /locus_tag="VYN16_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            570..1229
                     /locus_tag="VYN16_001744"
     CDS             570..1229
                     /locus_tag="VYN16_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1404242.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1254..2321
                     /locus_tag="VYN16_001745"
     CDS             1254..2321
                     /locus_tag="VYN16_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1404243.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGSLIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2424..3434
                     /locus_tag="VYN16_001746"
     CDS             2424..3434
                     /locus_tag="VYN16_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404244.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3446..5137
                     /locus_tag="VYN16_001747"
     CDS             3446..5137
                     /locus_tag="VYN16_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1404245.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5140..5850
                     /locus_tag="VYN16_001748"
     CDS             5140..5850
                     /locus_tag="VYN16_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1404246.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1985 a          823 c         1131 g         1980 t
ORIGIN      
        1 ttagtaaacg aaatggaaat aactgatcat agagtagata acttatttga aaaagggaaa
       61 aatgagataa aagatccatg tagtagattt agcaatagga catataaaag ctttagaaaa
      121 agtatctgaa aaaacagatg tttatatcta taacctatgc tcgggagaag gcactagtgt
      181 attacaactt gtaaatacat ttgaaagtgt taataagatc cctattcctt ataaaatagt
      241 tccaagacgt tcaggagacg ttgcaacttg ttatgcaaat gcagacaaag agcatataag
      301 gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac atggaattgg
      361 caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg ctgtattttg
      421 gaaaaattac ttaacctttt aatgaaatgc atatttaatg aaagataaat taaaaaaaat
      481 gtttgacagc gcttgacaaa ggaaacggtt tcattgtata atgtaaacat agtgacaccg
      541 atgtcaaata taaaaaagaa ggtctttata tgaaaaactt aacaaaaatt aagttcaaag
      601 agaatgggga atttaatcat ttccctggga atacagttgt agcaaatctt tatactaaac
      661 aagatttgat ggaagttgtt gatattattc aatcacgtta tagagaatta ccatttatcg
      721 ataagtttac tttaactcca aggaattcta ttcacatgac tgtaattgaa ttgttgtgcc
      781 atgaaaatcg tgaaacggag ttttggagca gtaatcttcc tctagataca cctttacagg
      841 aaatacatga ttactttgct aaacaacttg aaatttttcc attgttggat gaagaaattc
      901 atatgcgtat aactgaaatg ggaaaacaaa acatactagt tgaacctgca gatgaagctt
      961 ctgcaaagag attagaagaa attcgtactt atgtctcaga aaaagcaggt gtttgtttcc
     1021 ctaatcatga tagatatcaa ttccatattt caattgggta tcttcggatt cctctaaccg
     1081 aagaggagga agaagagttt actaaagtca gagcagaatt aactgaaatt ttattagaga
     1141 agattccaac tattactgta aaccgtattg actatactgt atttgaagat atgagacaat
     1201 ttgttccata tcacgaaaaa tttaaataaa ataataaaaa aggagaactt atcatgaaaa
     1261 aaatgaaagt ttggtctact gtacttgcaa cgggagttgc tcttactaca cttgctgctt
     1321 gctctggagg ttcaaattct acgactgctt cttcatctga agaaaaagct gataaaagtc
     1381 aagaattagt tatctattcg aactcagtct caaatggtcg tggtgattgg ttaactgcta
     1441 aagcaaaaga agctggtttt aatataaaaa tggttgatat cgctggcgct caattagcag
     1501 accgtgttat tgctgagaag aataatgcag ttgcagatat ggtatttgga attggtgctg
     1561 ttgactcaaa taaaattaga gatcaaaaat tactagtaca gtacaagcct aaatggttag
     1621 ataaaattga tcaatcttta tcagataaag ataattatta taatcctgtg attgttcaac
     1681 cattagtttt aattggggcg cctgatgtaa aagaaatgcc taaagattgg actgaattag
     1741 gtagtaagta taaaggtaaa tattcaattt atggtcttca aggtggtaca ggacgggcaa
     1801 ttctagcaag tatcttagtt cgttaccttg atgaaaaagg agatttaggc gtatcagaaa
     1861 aaggatggga agttgcaaaa gaatactttg caaatgccta tactcttcaa aaaggtgaaa
     1921 gctcagttgt taaagcgtta gataaagaaa gcccaattca atacggtatg atctggggtt
     1981 ctggatcgtt aattggacaa aaagaacaaa atgttgaatt caaagttatg actcctgaga
     2041 ttggtgtacc atttgtaact gaacaaacta tggttttaag cactagtaaa aaacaagcgt
     2101 tagctaaaga atttattgat tggtttggtc aatcagaaat tcaagtagaa tatagtaaaa
     2161 aatttggatc tattcctgca aataaagatg ccctaaaaga gttacctgaa gatacgaaaa
     2221 aatttgttga tcaagtgaaa ccacaaaata ttgactggga agctgttgga aagcatttgg
     2281 atgaatgggt agaaaaagct gaattagaat acgtacaata agaaataaga gttatttttt
     2341 atgtccaagt ctctctatta tttctaaatt taaggaagaa ataaagagag gcttttggtt
     2401 tagtaataga gggagttagt gagatgatta aatttgataa tattcaaatt aaatatggtg
     2461 attttgttgc aattgataat ctgaatttag atatacatga aggggaattc tttacatttc
     2521 ttgggccttc aggatgtggt aaatcaacta ctttgagagc attggtaggt tttttagatc
     2581 catcatcagg aagtattgaa gttaatggaa cagatgtcac tcatttggaa cctgaaaagc
     2641 gtggaattgg tattgtattt caatcttatg cgctatttcc aactatgact gtttttgata
     2701 atattgcatt tggtttaaaa gttaagaagg tagctccaga tgttattaaa gctaaagtat
     2761 cagcagtggc agcaaaaatt aagatctctg atcaacagtt acagcgtaat gtatcagaat
     2821 tatctggggg tcaacaacaa agggtagcat tggctcgtgc tctggttctt gaacctaaaa
     2881 ttctttgtct agatgaacca ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa
     2941 aagagttgaa aagacttcaa aaagagttag gtattactac tttatatgtt actcatgatc
     3001 aagaggaagc cttgacttta tctgatagaa ttgcagtctt taacaatgga tacatcgaac
     3061 aggtcggtac accagtagag atttatcata attctcaaac tgaatttgta tgtgatttta
     3121 ttggagatat taatgttttg accgatgaaa cagtccacga agtattattg aaaaatacaa
     3181 gcgttttctt agaggataaa aaaggataca ttcgattaga gaaagttcga ttcaatcgtg
     3241 aaactgaaca agattttatt ctaaaaggga caattattga tgttgagttt tctggagtta
     3301 caattcacta tacaataaaa gtttctgaaa gtcagattct taatgtaaca agtattgata
     3361 gtcaggctgc tattagatct gtcggagaaa gtgtggaatt atttatcaca ccatcagacg
     3421 ttctgcaatt ttaaggaggt gcagtatgcg tcataaatta aatttaaaag attggcttat
     3481 tcgtttagga ttaatctggt tcttagtaac atttattatt tatccaaact ttgatctagt
     3541 agtgaatgta tttgtaaaag gaggagaatt ttcccttgat gctgtacatc gtgttctaaa
     3601 atctcagagg gcacttcaga gtattatgaa cagttttaag ttagcatttt cactcattat
     3661 tacagttaat gtcgtaggta ttctttgtgt tctatttaca gagtactttg atattaaagg
     3721 tgctaaaatt ttaaaattag gttatatgac ctctttaatt tatggaggag tggttttagc
     3781 gactggatat aaatttgtct atggtcctta tggattgatt acaaaatttt tacaaaatgt
     3841 tatcccttct ttagactcta actggtttat tgggtatggt gcagtcttat tcattatgac
     3901 attttcagga actgctaatc atacattgtt tttaacaaat acaattcgaa gcgttgacta
     3961 tcacactatt gaggctgctc gaaatatggg agcaaaacca tttactgttt tccgaaaagt
     4021 agtgttacca accttaattc caactctatt tgcacttact attatggttt ttcttagtgg
     4081 tttatctgca gtagcagcac ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc
     4141 aatgattatt acatttgcag ggatggggaa ttctcgtgat ttagctgcct tacttgcaat
     4201 tattttaggt attgcaacta caattttgct tactatcatg aataagatag aaaaaggtgg
     4261 aaattatatt tctatctcta agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa
     4321 gccttggaat atcattgctc acattgtagc atatggattg ttcacagttt tcatgcttcc
     4381 actaattttt atagtattat actcatttac agatccagtt gcaattcaaa caggtaactt
     4441 aacattatca aactttactt tagaaaatta tcgcttattc tttagtaata gtgcggcatt
     4501 ctctccattc ttggtcagct ttatttattc tattattgct gcgacaacag caacaattct
     4561 cgcagttgta tttgctcgtg ttgtcagaaa acataaatct cgttttgatt tcttatttga
     4621 atatggtgct ctacttcctt ggttactacc aagtacactt ttagcagtaa gtttattatt
     4681 tacttttaat cagccacaat ttcttgtctt gaatcagatt ttggtaggta gtttggtaat
     4741 tctacttatt gcatatatag ttgtaaaaat cccattttct tatagaatgg tacgtgctat
     4801 tttatttagt gttgatgatg agatggaaga tgcagcaaga agtatgggtg cttcaccttt
     4861 ttatactatg atgaaggtta tcattccatt tattttaccg gttgttctct ctgttattgc
     4921 tttaaacttt aactctttat taactgactt cgacttatct gtattccttt accatcccct
     4981 agctcaacca ttaggtatta cgattcgatc tgcaggtgat gaaacagcaa catctaatgc
     5041 acaagctctg gtatttgttt atacaattgt tctgatgatt atttctggaa cggtattata
     5101 cttcacacaa agaccggggc gtaaagtaag gaaataatca tgacagccac tagtcttggg
     5161 ttatcaaata ttgaaatagt tgtcaggatt gttttatcag tagtcattgg tagtataatt
     5221 ggtttagaga gagggagcaa atcccagcct gcaggcatcc gaacttatag tattgtttgt
     5281 ctagctgcat gtttgattat gatgacgaat gaatacgtat cttataaatt tgggacagga
     5341 gatcctacac gattaggagc tcaagttata tcaggtgtgg gttttctagg cgctggaacg
     5401 attcttatta cagataaaaa gaaaattaca ggtctgacaa ctgcagcagg catttgggct
     5461 tcggcaggaa ttggattagc tattggagta ggtttttatg agggagctct tttagtagcc
     5521 atttctgttt ggggtgtgat atccatgttc caaccactaa aaaaatatct gcaaaatcgt
     5581 tctaaaatga ttgaattgta tatagtagtt aaatctacag aagcctataa tcgtgtattg
     5641 gtttattgtg cagaaaatgg tatcagaatg accgattcaa gaacagcgtt tggagatgtt
     5701 aattctgaca gaattgaata ctttgatgtt ccggacaaaa aaatagcgtc atttattact
     5761 ctggaattat caggtagatt tgaacatctt cgactgatgg aagaaattgc taatattgta
     5821 ggtgttattt atgtcgaaga aattagctga aatttagtaa acaaagggac agatgttttt
     5881 gtccttttgt tttttagatg ttttaatcat ctaattttt
//
LOCUS       DAVLTM010000108         5786 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLTM010000108 DAVLTM010000000
VERSION     DAVLTM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.109"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            65..1336
                     /locus_tag="VYN16_001749"
     CDS             65..1336
                     /locus_tag="VYN16_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1404247.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1447..2739
                     /locus_tag="VYN16_001750"
     CDS             1447..2739
                     /locus_tag="VYN16_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1404248.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2741..3583
                     /locus_tag="VYN16_001751"
     CDS             2741..3583
                     /locus_tag="VYN16_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1404249.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3836..4636
                     /locus_tag="VYN16_001752"
     CDS             3836..4636
                     /locus_tag="VYN16_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1404250.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4646..5632
                     /locus_tag="VYN16_001753"
     CDS             4646..5632
                     /locus_tag="VYN16_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1404251.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
BASE COUNT         1680 a         1311 c         1041 g         1754 t
ORIGIN      
        1 cataaaggaa aacgtttgcg tttccttatc aataaaactt gctattcttt aggaggaata
       61 cactatgtca tctaaattta tgaagagcgc tgcggtgctt ggaactgcta cacttgctag
      121 cttgctctta gtagcttgcg gaagcaaaac tgctgataag cctgctgatt ctggttcatc
      181 tgaagtcaaa gaactcactg tatatgtaga cgagggatat aagagctata ttgaagaggt
      241 tgctaaagct tatgaaaaag aagctggagt aaaagtcact cttaaaactg gtgatgctct
      301 aggaggtctt gataaacttt ctcttgacaa ccaatctggt aatgtccctg atgttatgat
      361 ggctccatac gaccgtgtag gtagccttgg ttctgacgga caactttcag aagtgaaatt
      421 gagcgatggt gctaaaacag acgacacaac taaatctctt gtaacagctg ctaatggtaa
      481 agtttacggt gctcctgccg ttatcgagtc acttgttatg tactacaaca aagacttggt
      541 gaaagatgct ccaaaaacat ttgctgactt ggaaaacctt gctaaagata gcaaatacgc
      601 attcgctggt gaagatggta aaactactgc cttcctagct gactggacaa acttctacta
      661 tacatatgga cttcttgccg gtaacggtgc ttacgtcttt ggccaaaacg gtaaagacgc
      721 taaagacatc ggtcttgcaa acgacggttc tatcgtaggt atcaactacg ctaaatcttg
      781 gtacgaaaaa tggcctaaag gtatgcaaga tacagaaggt gctggaaact taatccaaac
      841 tcaattccaa gaaggtaaaa cagctgctat catcgacgga ccttggaaag ctcaagcctt
      901 taaagatgct aaagtaaact acggagttgc aactatccca actcttccaa atggaaaaga
      961 atatgctgca ttcggtggtg gtaaagcttg ggtcattcct caagccgtta agaaccttga
     1021 agcttctcaa aaatttgtag acttccttgt tgcaactgaa caacaaaaag tattatatga
     1081 taagactaac gaaatcccag ctaatactga ggctcgttca tacgctgaag gtaaaaacga
     1141 tgagttgaca acagctgtta tcaaacagtt caagaacact caaccactgc caaacatctc
     1201 tcaaatgtct gcagtttggg atccagcgaa aaatatgctc tttgatgctg taagtggtca
     1261 aaaagatgct aaaacagctg ctaacgatgc tgtaacattg atcaaagaaa caatcaaaca
     1321 aaaatttggt gaataaaaaa tttgttcaag gggggtggaa atcaaatccc cctttgaatt
     1381 tatcaataga gacacaaata atttagcttt cttataaaaa agtagtatcc tatgaaagga
     1441 gttaatatgg aaaagcaaca acctagtaaa gcagccctgc tgtctatcat tcctgggtta
     1501 ggacagattt acaataaaca aaaagccaaa ggttttatct tccttggtgt aaccatcgta
     1561 tttgtccttt acttcctagc acttgcaacc cctgaattga gcaacctcat cactcttggt
     1621 gacaaaccag gtcgtgataa ttccctcttt atgctgattc gtggtgcctt ccatctaatc
     1681 tttgtaatcg tttatgtact cttttatttc tcaaatatca aagatgcaca tacgattgca
     1741 aaacgcatta acaatggaat tccagttcca cgcacactca aagacatgat caaagggatt
     1801 tatgaaaatg gcttccctta cctcttgatc attccatctt atgttgccat gaccttcgcg
     1861 attatcttcc cagttatcgt aaccttgatg atcgccttta ccaactacga cttccaacac
     1921 ttgccaccaa acaagttgtt ggactgggtt ggtttgacca actttacaaa catttggagc
     1981 ttgagtacct tccgttctgc ctttggttct gttctttctt ggactatcat ttgggctttg
     2041 gcagcttcta ctttacaaat cgtaattggt atcttcacag ctatcattgc caaccaacca
     2101 tttatcaaag gaaaacgtat ctttggtgtt attttccttc ttccttgggc tgtcccagcc
     2161 ttcatcacta tcttgacatt ctcaaacatg tttaacgata gtgtcggtgc tatcaacact
     2221 caagtattgc caatcttggc taaattcctt cctttccttg atggagctct tattccttgg
     2281 aaaacagacc caacttggac taagattgcc ttgattatga tgcaaggttg gctcggattc
     2341 ccatacatct acgttttgac tttgggtatc ttgcaatcta ttcctaacga cctttacgaa
     2401 gcagcttata ttgacggtgc caacgcttgg caaaaattcc gcaacatcac tttcccaatg
     2461 attttggctg ttgcggcacc tactttgatt agccaataca ccttcaactt taacaacttc
     2521 tctatcatgt acctcttcaa tggtggagga cctggtagtg tcggaggtgg agctggttca
     2581 accgatatct tgatctcatg gatctaccgt ttgacaacag gtacatctcc tcaatactca
     2641 atggcggcag ctgttacctt gattatctct atcattgtca tctcaatctc tatgatcgca
     2701 ttcaagaaac tacacgcatt tgatatggag gacgtctaag atgaataact caattaaact
     2761 caaacgtaga ctgactcaaa gccttactta cctttacctg attggtctat caattgtaat
     2821 tatctatcca ctgttgatta ccattatgtc agcctttaaa gcaggtaacg tctcagcctt
     2881 taaactagat actaatatcg acctcaattt tgataacttt aaaggcctct tcactgaaac
     2941 cttgtacggt acttggtacc tcaacacttt gattatcgcc ttaattacca tggctgttca
     3001 aacaagtatc atcgtacttg ctggttatgc ttacagccgt tacaacttct tggctcgtaa
     3061 acaaagtttg gtcttcttct tgatcatcca aatggtgcca actatggccg ctttgacagc
     3121 cttcttcgtt atggcgctta tgttgaacgc ccttaaccac aactggttcc tcatcttcct
     3181 ctacgttggt ggtggtatcc cgatgaatgc ttggctcatg aaaggctact tcgatacagt
     3241 gccaatgtct ttagacgaat ctgcaaaact agacggtgca ggacacttcc gccgcttctg
     3301 gcaaattgtt ctaccacttg ttcgcccaat ggttgccgta caagctctct gggccttcat
     3361 gggacctttc ggggactaca tcctctctag tttcttgctt cgtgagaaag aatactttac
     3421 tgttgccgta ggtctccaaa ccttcgttaa caatgcgaaa aacttgaaga ttgcctactt
     3481 ctcagcaggt gctatcctca tcgcccttcc aatctgtatt ctcttcttct tcctacaaaa
     3541 gaactttgtt tcaggactta caagtggtgg cgacaaggga taatttatcc ccgccaccct
     3601 ttttcatttt atactcttcg aaaatctctt caaaccacgt cagctttagc tccaacctca
     3661 aagttgtgct ttgagcaacc tgtggctagt ttgcactttg attttcattg attattagca
     3721 attgtcactg taaataatat ccttgtagca agcaattttt ctcctagact tgaaataaag
     3781 cgcatttctc tatataataa tactcatata gaaaacacct tttagaaaga tacctatgct
     3841 tccatatcca ttttcctatt tttcaagtat ttgggggttt cgtaagcccc tgtccaaacg
     3901 tttcgagctc aactggtttc aacttctctt taccagtatc ttccttatca gcttgtctat
     3961 ggtacccatt gctatccaaa acagctccca ggagacctat ccgctagaaa cttttatcga
     4021 taatgtctat gaacctctga cagataaggt tgtccaggat ctctctgaac atgctacaat
     4081 tgtcgatggc acattaactt atactggaac agctagtcaa gccccttctg ttgtgattgg
     4141 tccaagtcaa atcaaggaat tacctaagga cttgcaactg catttcgata caaatgagct
     4201 agtcatcagc aaggaaagca aggaactgac ccgcatctct taccgagcca ttcagactga
     4261 gagtttcaaa agcaaagaca gcttgaccca agcaatttct aaagactggt accaacaaaa
     4321 tcgtgtctat atcagcctct tcctagttct cggtgcgagc ttcctctttg gtttgaattt
     4381 ctttatcgtc tctctaggag ctagtcttct cctttatatc actaagaaat cacgcctctt
     4441 ttcatttaga acctttaaag agtgctatca ctttatcttg aactgtttag gattgccgac
     4501 tctgattaca cttattttgg gattatttgg ccaaaatatg acaaccctga ttactgtaca
     4561 aaatattctt tttgttctgt atctggtcac tatcttttat aaaacacatt tccgtgatcc
     4621 aaattaccat aaataggaga tttttatgcc cgttacgatt aaagacgtgg ccaaggctgc
     4681 tggtgtttcg ccttcaaccg taacccgtgt tattcaaaat aaatcaacca ttagcgacga
     4741 aacaaaaaaa cgcgttcgca aagctatgaa ggaactcaac taccacccaa acctcaacgc
     4801 tcgtagcttg gtaagcagct atactcaggt tatcggatta gttcttcctg atgactcaga
     4861 cgccttctac cagaatcctt tctttccatc ggttctacgt ggcatctctc aagtcgcatc
     4921 tgaaaaccac tatgccattc agatagcaac agggaaagat gagaaggagc gtctcaacgc
     4981 tatttcacaa atggtctacg gcaagcgtgt agatgggcta atctttctct atgcccaaga
     5041 agaagaccct ctcgtaaaac tcgtcgcaga agaacagttc cccttcctta tcctaggtaa
     5101 atctctatct cctttcatcc cacttgtcga caacgacaat gttcaagctg gttttgatgc
     5161 gactgaatat ttcatcaaaa aaggctgcaa acgcattgcc tttatcggag gaagtaaaaa
     5221 gctcttcgtg accaaagacc gtttaacagg ctatgaacag gcgcttaaac attacaaact
     5281 taccactgac aacaatcgca tctactttgc cgacgagttt ctggaagaaa agggctataa
     5341 atttagcaag cgattattca agcacgatcc acaaattgac gctatcatca caaccgatag
     5401 cctcctagct gaaggtgttt gtaactatat tgccaaacac cagctggatg tccctgttct
     5461 cagctttgac tcggttaatc ccaagctcaa cttggcagcc tatgtcgata tcaatagttt
     5521 agagcttggt cgtgtttccc ttgaaactat tctccagatt attaatgata ataaaaacaa
     5581 taaacaaatt tgttaccgtc aattgatcgc ccacaaaatt atcgaaaaat aagagactgg
     5641 gcaaagagtc gttaaaagca aaaacgcata ctatcaggta ttgaaaaaac ttgatactat
     5701 gcgttttatt gtgggaagat ttacttcctt ttctactgaa attgagtctt ttcccaagat
     5761 ctttttatac tcaatgaaaa tcaaag
//
LOCUS       DAVLTM010000109         5755 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLTM010000109 DAVLTM010000000
VERSION     DAVLTM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.110"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            109..636
                     /locus_tag="VYN16_001754"
     CDS             109..636
                     /locus_tag="VYN16_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1404252.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            648..1163
                     /locus_tag="VYN16_001755"
     CDS             648..1163
                     /locus_tag="VYN16_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1404253.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1160..1627)
                     /locus_tag="VYN16_001756"
     CDS             complement(1160..1627)
                     /locus_tag="VYN16_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1404254.1"
                     /translation="MSRSQLTILTTICLIEDLETQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMESPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(1680..2189)
                     /locus_tag="VYN16_001757"
     CDS             complement(1680..2189)
                     /locus_tag="VYN16_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404255.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALDHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SKIILKSRS"
     gene            complement(2155..2718)
                     /locus_tag="VYN16_001758"
     CDS             complement(2155..2718)
                     /locus_tag="VYN16_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404256.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(2728..4716)
                     /gene="uvrB"
                     /locus_tag="VYN16_001759"
     CDS             complement(2728..4716)
                     /gene="uvrB"
                     /locus_tag="VYN16_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1404257.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVVAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(4793..5746)
                     /locus_tag="VYN16_001760"
     CDS             complement(4793..5746)
                     /locus_tag="VYN16_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1404258.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         1692 a         1303 c         1036 g         1724 t
ORIGIN      
        1 actataactt ttaactaaaa aatgatctat aaacccaagt aaattcttgc tttattgtct
       61 ggtttattgt accatactag tgtatatgca gttaaaaagg agattcttgt gaatacacgg
      121 aaaaagacac aatttatgac aatgacagcc cttttaacgg ctattgcgat tttgattcca
      181 attgttatgc ctttcaagat tgtcattcca cctgcttcct atactttggg gagccacatc
      241 gctattttta tagccatgtt cttgtcgccc ttgatggcag tttttgtcat cctagcctct
      301 agttttggat ttttgatggc tggctatccc atggttatcg tttttcgggc cttttcccat
      361 atatcttttg gtgctttagg agctctttac ctacaaaaat tccccgatac cctagataaa
      421 ccaaaatctt cctgggtttt caattttgtt ttggctgttg ttcatgccct tgctgaagta
      481 ttagcctgtg tcctttttta cgcaacttcg ggtaccaatg tagaaaacat gttctatgtt
      541 ctatttgtac tagttggatt tggcacaatt atccatagta tggtagacta tacattagca
      601 ctagctgtct ataaagtgct tcgaaaacgc cgttaaaagg aaaagctatg acaaaagatc
      661 gcaaacaagc cctgctccaa ctcttgaaag aagctcctaa agcccttaat ggccaaagtt
      721 tggcggaaca ttttcatgtc acacgtcagg tcattgtgca ggacattgca attttgagag
      781 ccgatggcgc tcctatccta tccaccaatc gtggctacat ctataagcaa attgatacca
      841 atccttatgt tcacaaactt ttcaaagtga aacatgaagt tgaagaaatc ggtcaggaac
      901 tccttgctat tgtcgataat ggtgggcatg tccagaatac cttgattgac catcccgttt
      961 atggagaaat tgaaaccttg cttaaactgt cttgtcgcag agatgtgcaa cattttctag
     1021 aacaagtcga gcattctgac tttagacctc tatctgaatt gacagatggc atccattacc
     1081 acctagtcga agccgaaaca caacaagacc tccactatat cgaggaggcc ttggatcagc
     1141 taggttattt agtaaaagac tagaagattt tcttctccca atcatcttct gtacggcgag
     1201 ggtagaaaaa ttcagatttg tctggagatt ccatcatctc catcaaaggt agcatatcat
     1261 aggccagatc taagtttgga atctggtctt tttgcaccca agaaacttct ccttcatctg
     1321 aagagcgaag ggtaccagag aactcagtag ccttataaca aacgacaata tagcgcccac
     1381 ctgtatctag tggccaattt ttaatgccga caagttgagg attttggata gtcaaccctg
     1441 tttcttcata tatttcacga atgacagact ccgcgaaaga ctcgccattt tctacatgac
     1501 ctcctggaaa ggcataacca gaccagcgat tggtttcagg agcgcggtac tgcatcacca
     1561 cgcgctgcgt ttcgaggtct tcaatcagac aaatggttgt taaaatcgtt aattgggaac
     1621 gggacataaa tttactcgct ttctaattta ttaaattttc agacttggta ccagtgccat
     1681 tagcttctac tttttaaaat tattttagaa tagaatacca ttcgtttttc ctttataggc
     1741 tttccaagtt caagtatctt ttcttgtcca tcaaaagtaa aacccatttt ttgatagaaa
     1801 gcaatggctc gcttgttatc tttcaatacc cataagaaaa tttcagaaaa atgatcaaga
     1861 gcagtcaaag ctgctttcat taacttttgt gctatgcctt ttccgtaata gtcttttaaa
     1921 acatataaag caataatttc accagcttga atagtctcat cacgaaagtt accataactg
     1981 ataaaaccaa ctaccttcag atcacctatc gcaatcaatg tattttctgg atacttttga
     2041 ctaaagagtc gacatctttc taatgtcatt gtctcctgaa attctgcagg caaaaggtca
     2101 tcataagcct ctctccacgt ttgccagtga acgagggatt taccttctat ctcttcagga
     2161 gtttccattg atttgataat aaccgtcatt tattttctcc cagtcttctc tcaaaatacc
     2221 atatttaata ctatcaaaat atttaccttg ataataacga acttttggaa tatgagcttc
     2281 ttttttcatt cttaattttt cagcaagttt catcatacca agattccctg accaagttgt
     2341 caaacccaga tgctccaact ccaagtaatc ctgaaacgtc ctatctatcc actgcaacat
     2401 agcaactttc ccaataccag tgttccagaa ttttttatca taaataccaa ttcccaattc
     2461 catccatctt gtttgtttac atacccaata acgcgaaaca gtcccaacta gtttatcatc
     2521 aacaaaaata ccaaggcgat ttgagttgct taaaatggat tctgattttt gtagttcgaa
     2581 ttctttaaaa tttgaaaaat actgataatc atcataatag ggagcatcat actgtttcca
     2641 aactggatta agttgtgaat aagccatttc ccataaagac atcaaatctt ctctctttaa
     2701 ctttcttaaa taaatcatac tattccccta atccaaggcc ttgacttcca acatcatatc
     2761 acggatctga gccgccagtt caaagtcaag cacttcgacg gcttcttgca tttgtttttc
     2821 tagtttcttg acgagttctt tgcgctcttg tttgttgagg ctattgatgt cgacttcctt
     2881 gtcctcttcc ttagcaactg ccttggtcac ggcaatcagg tcacggattt ctttcttgat
     2941 tgtctgtggt acgataccat gctcttcatt ataggccatc tgaattttcc gacggcgggc
     3001 agtttcatcg atagcacgtt gcatagactg ggtaaccgtg tctgcataca tgatgacatg
     3061 gccttcactg ttacgagcag cacgtccaat agtctggatg agtccacgtt cgttgcgaag
     3121 gaaaccttcc ttgtcagcat cgagaatagc tacgaggctc acttcaggaa cgtcaattcc
     3181 ttcacggagc aggttaattc cgaccaagac atcaaagaca cccaagcgca ggtcacggat
     3241 aatctccgtc cgttccaagg tcttgatatc cgagtgcatg tacttgacct tgatgcccat
     3301 ttccttgaaa tagtcggtca agtcctctgc cattttcttg gtcaaggtgg tgataaaggt
     3361 tcgttcattc ttttcaacac gggcattgat ttcacctaag agatcatcaa tctgtcccat
     3421 agtcggacgg acttccacct caggatccaa aagccctgtc ggtcgaatga tttgctcaat
     3481 caccgtctcg gtctgttcat tttcatagtc accaggtgtc gctgaaacgt aaacaatctg
     3541 atgcacatgg ctttcaaact cctcacgacg gagaggacga ttgtccaagg cagacggtaa
     3601 acggaaacca taatttacta gcatttcctt acgcgaacgg tctccattgt acatgccttt
     3661 tatttgtccc atagtcatat gactctcgtc aatcataatc aaaaaatcat ctgggaagaa
     3721 gtcgagaagc gtataaggag gctcaccttc actacgtcca tccatgtggc gagaataatt
     3781 ttcaacccca tttgtatagc ccatctcgcg cagcatttcg atatcgtact ctgtccgttg
     3841 tttcaagcgc tgggcttcaa gcaatttacc ttccttctca aagacagcta actgctcttc
     3901 taattctgct tgaatcttgg caacggcaac ttccatatgg tcgtcattgg tcacaaagtg
     3961 agtagctggg aaaatcgcca aatgatccac ttctcccaac acctgacctg tcagagcctc
     4021 aacttcacga atacggtcaa tttcgtctcc aaaaaattct actcgaaagg catgttcatc
     4081 tcgggaagct gggaaaatct ctaccacatc cccacgaacg cgaaatcttc cgcgttggaa
     4141 atcaatatca ttacgttcaa actgaatatc gaccaagtca ttcaagagtt tatcacgaga
     4201 aatctctaga ccaggacgga gactaacgac actatcagcg tattccttgg gcgaacccaa
     4261 accatagata caagagactg aggccacgac aataacatca ttacgctcca aaagggctga
     4321 ggtagctgag tggcgaagct tgtcaatctc gtcattgaca gaactatcct tctcaatata
     4381 ggtatcgcta gaagggacat aggcctctgg ctggtaataa tcatagtagg atacgaaata
     4441 ctcaactgca ttttcaggga aaaattcctt aaactcccca tagagctgac cagccagagt
     4501 tttattgtgg gcaacaacca gagttggttt attgacttta gaaatgacct gactcatagt
     4561 ataggtcttc cctgttccag tcgcccccat cagaatctga gctttttctc ccccctcaat
     4621 gttatccacc aactgctcga tagcttgggg ttgatctcct gatggttgat attttgatac
     4681 tagtttaaat tgattatctg taatgtgatt gatcataaga atcctctctc catgtgctat
     4741 tcctatttta gcatacttat agtattttca cgaagaaaag gacggaccga agtcacatcc
     4801 tttcattttc tttctttaat ttattagcaa gatagacaat cagcagtagt aataccagac
     4861 ttgtcataat ggaaccttct atgccaaaag aaccacctga tagccaatct tgattgcctt
     4921 gtggtaaaaa ggtcatcaga gacgttcctg acggttgacc actaactaaa atcccaaaga
     4981 gattaccctg agcaaaattc caagcaccat gaatacctgc aacaccccaa actgtatcag
     5041 ttttgagaag ataaagagcc atggcaactc cgaataaaaa gagatttact agagatagag
     5101 gggtgaggcc agaattgccc atatgaagca gggtaaagaa caggctagat ataagaatag
     5161 ctagttttag attggttctt gaggccaatt gaggaagtag ccaagcacgg gccaccactt
     5221 cttctgctgt cccctgtaaa atccaaaatg ggatagtaaa gacgacaaag gcaagagaat
     5281 aaggattcaa gtgaatggat tccaaacgat attgacctaa gaccactaaa cctaacaagg
     5341 tcagaagaaa aagtgccaag cctagactaa atcctttcag aagattgctg aggaaattct
     5401 ctctataaaa tcccaaggtt cgaataggtc ttttctcaac ttttcttgtc catctgaaca
     5461 cagtgttaag aataaaacca aaggccagta atcctaaaat taaacgaaag tcacttgttt
     5521 tatccgtcaa gctctgatgc aaggtctgca agtagcttgc aagattttga ccagcctctc
     5581 caaaatttct agcaagtccg atgagagcta acaagccaat accatacaaa gtaaaagcca
     5641 caaactctcc tatgaagaca aaaacaaagg ctaacaaggg gcttgtgtaa atgtttaaac
     5701 tcatttttga agcttggaag tctttaaata ttcttttgtt cttcatatcc ccgtc
//
LOCUS       DAVLTM010000110         5533 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLTM010000110 DAVLTM010000000
VERSION     DAVLTM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.111"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            17..649
                     /locus_tag="VYN16_001761"
     CDS             17..649
                     /locus_tag="VYN16_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1404259.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            693..1349
                     /locus_tag="VYN16_001762"
     CDS             693..1349
                     /locus_tag="VYN16_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1404260.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMTGSFVASVREACLEILYQIRKTCFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            1368..2000
                     /locus_tag="VYN16_001763"
     CDS             1368..2000
                     /locus_tag="VYN16_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1404261.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVFRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            2028..2462
                     /locus_tag="VYN16_001764"
     CDS             2028..2462
                     /locus_tag="VYN16_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1404262.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            2474..3511
                     /gene="leuB"
                     /locus_tag="VYN16_001765"
     CDS             2474..3511
                     /gene="leuB"
                     /locus_tag="VYN16_001765"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1404263.1"
                     /translation="MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNIYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            3508..3777
                     /locus_tag="VYN16_001766"
     CDS             3508..3777
                     /locus_tag="VYN16_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1404264.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            3781..4140
                     /locus_tag="VYN16_001767"
     CDS             3781..4140
                     /locus_tag="VYN16_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1404265.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            4359..4502
                     /locus_tag="VYN16_001768"
     CDS             4359..4502
                     /locus_tag="VYN16_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404266.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            4521..4775
                     /locus_tag="VYN16_001769"
     CDS             4521..4775
                     /locus_tag="VYN16_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404267.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            4765..4926
                     /locus_tag="VYN16_001770"
     CDS             4765..4926
                     /locus_tag="VYN16_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404268.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQREYY"
     gene            5091..>5533
                     /locus_tag="VYN16_001771"
     CDS             5091..>5533
                     /locus_tag="VYN16_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404269.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRST"
BASE COUNT         1749 a          945 c         1256 g         1583 t
ORIGIN      
        1 attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt tacttgaaaa
       61 agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag ttggtgggac
      121 aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact acgatacaac
      181 catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc tagaaattgc
      241 tatcatgcta gaagacattc gtttgactgc tcaggctgga agtcaagggg ttgtatttgg
      301 agctttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa ttgctgcatc
      361 aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg aagatcaacc
      421 ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc gtgctggtgt
      481 gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg agtacgctaa
      541 aggtaaaatt gaaattctac caggtggggg gattgacctt gacaaccgtc aaacctttat
      601 cgaccaggtg ggggtaacac aattgcatgg tactaaggtt gttttttaaa aaatagaaag
      661 gaactgctag ctttgggtag cagttttcac ttatgtttga aatttttaaa tcctatcaat
      721 ttaatcaaga aaaggctcat gattatggtt ttatagaaaa tagcgaagtc tggacatata
      781 gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct gataatgtga
      841 actttcaagt ctttgaccaa gagactggtg acctctatcc tcacgttcat atggaaagta
      901 tgacaggaag ttttgttgca agtgtccgtg aggcttgttt ggagattctt taccagatac
      961 ggaagacttg ttttgatgtg caagatttta tctgtcatca gactaagcgt atcatgactc
     1021 aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg cctgatacag
     1081 ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa atctcttgga
     1141 ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag catgaccaag
     1201 tagctaattt gctttcacaa aaggggattt atccagcctt ccatatgagc aagcgctact
     1261 ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa ttgatagaaa
     1321 aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg aactttcaat
     1381 tagctaaata ttctttactg aagaaatttt cagaaaatat aggatttacc acactagagg
     1441 aatatggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt cagatcatct
     1501 attcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt gctgctgtca
     1561 agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga gctgaaaatg
     1621 ctgctttgga agaaattgca gtggctctca atattcgcca agattactac caagtagaaa
     1681 ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttttcgc ttctctggta
     1741 ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccac gaatctggta
     1801 ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca cctgaattgg
     1861 ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt gagaaactga
     1921 gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct aagttcaagg
     1981 cactggccga taagaagtaa gaaatcacag atgcagatac tcgagctttg gtagctggaa
     2041 ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa actcatgcag
     2101 ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa gtcgaattta
     2161 atgcgacagg gcaaggttcc gttgaagcaa tctttaatgc tatcgataag ttctttaacc
     2221 aatctgttcg tttggtgtcc tacactatcg atgcggtaac agatggaatc gatacccagg
     2281 atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat gcagcagggc
     2341 ttgattttga tgtgttgaag gcttctgcta ttgtctatat aaacgctaat acctttgttc
     2401 aaaaagagaa tgcaggtgag atgggacgca gtgtttctta ccacgatatg cctagtgtgt
     2461 aaaggagaag gctatggcaa agaaaatagt agctctagca ggagacggaa ttggcccaga
     2521 aatcatggag gttggtttag aagttctgga ggctctagct gaaaaaacag gttttgacta
     2581 tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcatggc ctcccttacc
     2641 tgatgaaacc cttaaggcaa gtagggaagc agatgctatc ctactagcag ctatcggtag
     2701 tcctcagtat gatggagcag tggttcgccc tgaacaaggc ctgatggctc tccgtaagga
     2761 actcaatatt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca agcatttgtc
     2821 accactcaaa ctggaacgaa ttgctggtgt agactttgtc gtggtgcgtg aattgacagg
     2881 cgggatttac tttggagatc atattcttga agagcgcaat gcgcgtgata tcaacgacta
     2941 tagctatgag gaagtggagc ggattattcg caaagccttt gaaattgcaa gaaatcgcag
     3001 aaaaatcgtt actagtatcg ataagcaaaa tgttctagcg acctcaaaac tctggcggaa
     3061 ggtagctgag gaagtcgcac aggatttccc agatgtaacc ttggaacatc agctggtaga
     3121 ctcagctgct atgcttatga ttaccaatcc tgctaagttt gatgttattg taacggagaa
     3181 tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac ttggggttat
     3241 gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattc acggttcagc
     3301 acctgatatt gcaggtcaag gaattgccaa tcctatttcc atgattttat cagttgtcat
     3361 gatgttgaga gatagtttcg gacgttatga ggatgcagag cgtatcaaac gtgctgttga
     3421 gacaagtctg gcggcaggaa ttttaacgag agatatagga ggtcaggctt caacaaagga
     3481 aatgacggaa gctattattg caaggttatg aagttagacg aaaaaattac tctagtcctt
     3541 ttgatttgga atgtcatcat tttcttgatt tatggcattg acaaattcaa ggcaagaaga
     3601 agagcttggc gcatccaaga gaaaatctta ctcattttag cccttacttg tggtggtttt
     3661 ggggcctggt tagctggaat cacttttcac cacaagactc gaaaatggta ctttaaaaca
     3721 gtttggtttc ttgggatggt gactacacta gtagccttat attttatttg gaggtaatgg
     3781 atggcagggt cttcgaggga acacgctgct tgggctctag cgaactatgg ttttaaggtc
     3841 gtgattgcag gaactttcgg tgacattcat tacaataatg aactcaataa tggcatgttg
     3901 ccaatcgttc agcctagaga ggttagagag aaactagccc agctaaaacc aaccgaccat
     3961 gtaactgtgg acttggaaca acaaaaaatc atctcaccag ttgaagaatt caccttcgag
     4021 atagatagcg agtggaaaca taaactccta aatagtttgg atgatatcgg tattaccttg
     4081 cagtatgaag agttgattgc tgcttatgaa aaacaacgac cagcctactg gcaggattag
     4141 aaaaaataga aaaggaaata tagtaaactg aaataagatg taaacaaatg aattagagct
     4201 taacatccat ttccagcaat tttttagaaa ctacagtgga ctattctgga ttcaacacat
     4261 tataaaatta tgacaaaaca cattcacaag aaggctacga cattttaaaa ggtgagggtg
     4321 gatgtatcgt ttgccctact aaagttggtt acattatcat gaccagtgat aaggcaggac
     4381 ttgagcgtaa gttcgcagcc aaagaacgta agcgtaacaa accaggtgtt gttctctgcg
     4441 gtagcatgga tgaactttgc gctttagcgc aactcaaccc agaaattgaa gcattctact
     4501 aaaaacattg ggatgaagat attcttcttg gttgtatcct tccttggaaa ccagaagcct
     4561 ttgaaaaact caaagcatac ggggatggcc gtgaagaact tatgactgat gtacgtggta
     4621 ctagctgttt tgttatcaag tttggaaaag caggtgaaca attggctgcc aagctttggg
     4681 aagaaggtaa aatggtctac gcctcatatg cttcaatgac aaaacgattg aaactcgcta
     4741 tgagagcaag gtgtaatggt gtctatggtc gataaggacg gcaaactcat cccagaacaa
     4801 ggaggagcac gttcaacctc accagcacca gttgtgatcc gtaaagggct tgacattgat
     4861 aaaatcatga tgcacctgtc agacaccttt aactcatggg attaccgtca gagagagtat
     4921 tattaggata aaaaagaagt ctagtgttat gagatattaa agctcctaac actgggcttt
     4981 ttgtttagaa tttcttttct ttttctatag gatatggtat tctatgtaga aaatatatgt
     5041 taataagtaa tgccaatatt taaacatcat tagtaaaagg agttagattg atgaataaaa
     5101 gaaaagttag tttagaagat ttttataaat ggtatagtct aaataaagaa gagttattaa
     5161 ataaggcaac tgttggtgaa aagtttaatg ataaattaaa agaagagttt ctccaggaat
     5221 ggcctttgga taggatttta acaatgtcaa tcgatgaata tgtaatagga aagggacagc
     5281 aaaataagtc tttatgctac gctcttgaga agggaaaata caaaaatcta tttcttggaa
     5341 tttctggtgg ctcagcttca aaatttggta tttattggaa taaaaaaaca aacaaatata
     5401 aagatcaagc taataatgag atttcagagt tggatcagcg attttcaaaa ttaaaatcag
     5461 atttgtatga aattatcaaa gaaggtattc gttttaactt tgaaaatcct atttttgata
     5521 tgaaaagatc aac
//
LOCUS       DAVLTM010000111         5449 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLTM010000111 DAVLTM010000000
VERSION     DAVLTM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.112"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            84..>5449
                     /locus_tag="VYN16_001772"
     CDS             84..>5449
                     /locus_tag="VYN16_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1404270.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARVKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDDRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRV"
BASE COUNT         2145 a          755 c         1068 g         1481 t
ORIGIN      
        1 ttagtaaaac tttttataac aaaggctagc aaagttaaag tttattatct attggaagtt
       61 aaataaatat gtaaggaatt aaaatgaaaa aaagtacagt attgtcatta accacagctg
      121 cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca tctagctctg
      181 aagatgcttt aaacatctct gataaagaaa aagtagtatt agataaggaa acagaaaata
      241 aagagaaaca tgaagatatt cataatgcta tagaaacttc acaggatttt aaagagaaga
      301 aaacagcagt cattgaggga aaagaagttg ttagtaaaaa tcctgtgaca gacaataaca
      361 ctagcaatga agaagcaaga gtcaaagaag attccaatca atcccaagga gatcatacgg
      421 actcatttgt gaataaaaac acagaaaatc ccaaaaaaga agataaagtt gtctatattg
      481 ctgaatttaa agataaagaa tctggagaaa aagcaatcaa gcaactatcc aatcttaaga
      541 atacaaaagt tttatatact tatgatagaa tttttaacgg tagtgccata gaaacaactc
      601 cagataactt ggacaaaatt aaacaaatag aaggtatttc atcggttgaa agggcacaaa
      661 aagtccaacc catgatgaat catgccagaa aggaaattgg agttgaggaa gctattgatt
      721 acctaaagtc tatcaatgct ccgtttggga aaaattttga tggtagaggt atggtcattt
      781 caaatatcga tactggaaca gattatagac ataaggctat gagaatcgat gatgatgcca
      841 aagcctcaat gagatttaaa aaagaagact taaaaggaac tgataaaaat tattggttga
      901 gtgataaaat ccctcatgcg ttcaattatt ataatggtgg caaaatcact gtagaaaaat
      961 atgatgatgg aagggattat tttgacccac atgggatgca tattgcaggg attcttgctg
     1021 gaaatgatac tgaacaagat atcaaaaact ttaacggcat agatggaatt gcacctaatg
     1081 cacaaatttt ctcttacaaa atgtattctg acgcaggatc tgggtttgcg ggtgatgaaa
     1141 caatgtttca tgctattgaa gattctatca aacacaacgt tgatgttgtt tcggtatcat
     1201 ctggttttac aggaacaggt cttgtaggtg agaaatattg gcaagctatt cgggcattaa
     1261 gaaaagcagg cattccaatg gttgtcgcta cgggtaacta tgcgacttct gcttcaagtt
     1321 cttcatggga tttagtagca aataatcatc tgaaaatgac cgacactgga aatgtaacac
     1381 gaactgcagc acatgaagat gcgatagcgg tcgcttctgc taaaaatcaa acagttgagt
     1441 ttgataaagt taacataggt ggagaaagtt ttaaatacag aaatataggg gcctttttcg
     1501 ataagaataa aatcacaaca aatgaagatg gaacaaaagc tcctagtaaa ttaaaatttg
     1561 tatatatagg caaggggcaa gaccaagatt tgataggttt ggatcttagg ggcaaaattg
     1621 cagtaatgga tagaatttat acaaaggatt taaaaaatgc ttttaaaaaa gctatggata
     1681 agggtgcacg cgccattatg gttgtaaata ctgtaaatta ctacaataga gataattgga
     1741 cagagcttcc agctatggga tatgaagcgg atgaaggtac taaaagtcaa gtgttttcaa
     1801 tttcaggaga tgatggtgta aagctatgga acatgattaa tcctgataaa aaaactgaag
     1861 tcaaaagaaa taataaagaa gattttaaag ataaattgga gcaatactat ccaattgata
     1921 tggaaagttt taattccaac aaaccgaatg taggtgacga aaaagagatt gactttaagt
     1981 ttgcacctga cacagacaaa gaactctata aagaagatat catcgttcca gcaggatcta
     2041 catcttgggg gccaagaata gatttacttt taaaacccga tgtttcagca cctggtaaaa
     2101 atattaaatc cacgcttaat gttattaatg gcaaatcaac ttatggctat atgtcaggaa
     2161 ctagtatggc gactccaatc gtggcagctt ctactgtttt gattagaccg aaattaaagg
     2221 aaatgcttga aagacctgta ttgaaaaatc ttaagggaga tgacaaaata gatcttacaa
     2281 gtcttacaaa aattgctcta caaaatactg cgcgacctat gatggatgca acttcttgga
     2341 aagaaaaaag tcaatacttt gcatcaccta gacaacaggg agcaggccta attaatgtgg
     2401 ccaatgcttt gagaaatgaa gttgtagcaa ctttcaaaaa cacggattct aaaggtttgg
     2461 taaactcata tggttccatt tctcttaaag aaataaaagg tgataaaaaa tactttacaa
     2521 tcaagcttca caatacatca aacagacctt tgacttttaa agtttcagca tcagcgataa
     2581 ctacagattc tttaactgac agattaaaac ttgatgaaac atataaagat gaaaaatctc
     2641 cagatggtaa gcaaattgtt ccagaaattc acccagaaaa agtcaaagga gcaaatatca
     2701 catttgagca tgatactttc actataggcg caaattctag ctttgatttg aatgcggtta
     2761 taaatgttgg agaggccaaa aacaaaaata aatttgtaga atcatttatt cattttgagt
     2821 cagtggaaga aatggaagct ctaaactcca acgggaagaa aataaacttc caaccttctt
     2881 tgtcgatgcc tctaatggga tttgctggga attggaacca cgaaccaatc cttgataaat
     2941 gggcttggga agaagggtca agatcaaaaa cactgggagg ttatgatgat gatggtaaac
     3001 cgaaaattcc aggaacctta aataagggaa ttggtggaga acatggtata gataaattta
     3061 atccagcagg agttatacaa aatagaaaag ataaaaatac aacatccctg gatcaaaatc
     3121 cagaattatt tgctttcaat aaccaaggga tcaacgctcc atcatcaagt ggttctaaga
     3181 ttgctaacat ttatccttta gattcaaatg gaaatcctca agatgctcaa cttgaaagag
     3241 gattaacacc ttctccactt gtattaagaa gtgcagaaga aggattgatt tcaatagtaa
     3301 atacaaataa agagggagaa aatcaaagag acttaaaagt catttcgaga gaacacttta
     3361 ttagaggaat tttaaattct aaaagcaatg atgcaaaggg aatcaaatca tctaaactaa
     3421 aagtttgggg tgacttgaag tgggatggac tcatctataa tcctagaggt agagaagaaa
     3481 atgcaccaga aagtaaggat aatcaagatc ctgctactaa gataagaggt caatttgaac
     3541 cgattgcgga aggtcaatat ttctataaat ttaaatatag attaactaaa gattacccat
     3601 ggcaggtttc ctatattcct gtaaaaattg ataacaccgc ccctaagatt gtttcggttg
     3661 atttttcaaa tcctgaaaaa attaagttga ttacaaagga tacttatcat aaggtaaaag
     3721 atcagtataa gaatgaaacg ctatttgcga gagaccaaaa agaacatcct gaaaaatttg
     3781 acgagattgc gaacgaagtt tggtatgctg gcgccgctct tgttaatgaa gatggagagg
     3841 ttgaaaaaaa tcttgaagta acttacgcag gtgagggtca aggaagaaat agaaaacttg
     3901 acaaagacgg aaataccatt tatgaaatta aaggtgcggg agatttaagg ggaaaaatca
     3961 ttgaagtcat tgcattagat ggttctagca atttcacaaa gattcataga attaaatttg
     4021 ctaatcaggc tgatgaaaag gggatgattt cctattatct agtagatcct gatcaagatt
     4081 catctaaata tcaaaagctt ggcgagattg cagaatctaa atttaaaaat ttaggaaatg
     4141 gaaaagaggg tagtctaaaa aaagatacaa ctggggtaga acatcatcat caagaaaatg
     4201 aagagtctat taaagaaaaa tctagtttta ctattgatag aaatatttca acaattagag
     4261 actttgaaaa taaagactta aagaaactca ttaaaaagaa atttagagaa gttgatgatt
     4321 ttacaagtga aactggtaag agaatagagg aatacgatta taaatacgat gataagggaa
     4381 atattattgc ttatgacgat ggtagtgcct tacaatatga aactgaaaaa cttgacgaaa
     4441 taaaatcaaa aatttatggt gttctaagcc catctaaaga tggacacttt gaaattcttg
     4501 gaaagataag caatgtttct aaaaatgcca aggtatatta tggaaatagc tataaatcga
     4561 tagaaatcaa agcgactaag tatgattcac attcaaaaac gatgatattt gatttatacg
     4621 ctaatattaa tgatattgta gatggattag cttttgctgg agatatgaga ttatttgtta
     4681 aagatgatga tcgaataaaa gctgaaacta agattagaat gcctgaaaaa aataaggaaa
     4741 ctaaagcaga atatccctat gtatcaagtt atgggaatgt aatcgaatta ggagaaggag
     4801 atctttcaaa gaacaaacca gacaatttaa ctaaaatgga atctggtaaa atctattctg
     4861 attcagaaaa acaacaatat ctgttaaagg ataacatcat tctaagaaaa ggctatgcac
     4921 taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat ggagtctata
     4981 gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagtc ctttcagaaa
     5041 caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa gctgtattaa
     5101 tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt aaaatgaacg
     5161 ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct aaacttgtat
     5221 tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta gaagctataa
     5281 aagaagatgg ttccatgtta tttattgata ccaaaccagt aaacctttca atggataaga
     5341 actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa ttttatttaa
     5401 gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaa
//
LOCUS       DAVLTM010000112         5436 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLTM010000112 DAVLTM010000000
VERSION     DAVLTM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.113"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(129..200)
                     /locus_tag="VYN16_001773"
     tRNA            complement(129..200)
                     /locus_tag="VYN16_001773"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Arg,seq:cct)
     gene            complement(242..589)
                     /gene="rplS"
                     /locus_tag="VYN16_001774"
     CDS             complement(242..589)
                     /gene="rplS"
                     /locus_tag="VYN16_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1404271.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(708..1037)
                     /gene="crcB"
                     /locus_tag="VYN16_001775"
     CDS             complement(708..1037)
                     /gene="crcB"
                     /locus_tag="VYN16_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1404272.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1031..1405)
                     /gene="crcB"
                     /locus_tag="VYN16_001776"
     CDS             complement(1031..1405)
                     /gene="crcB"
                     /locus_tag="VYN16_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1404273.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1402..1668)
                     /locus_tag="VYN16_001777"
     CDS             complement(1402..1668)
                     /locus_tag="VYN16_001777"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1404274.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1784..2227)
                     /locus_tag="VYN16_001778"
     CDS             complement(1784..2227)
                     /locus_tag="VYN16_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1404275.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2331..3266
                     /locus_tag="VYN16_001779"
     CDS             2331..3266
                     /locus_tag="VYN16_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1404276.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3320..3604
                     /locus_tag="VYN16_001780"
     CDS             3320..3604
                     /locus_tag="VYN16_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1404277.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3661..4149)
                     /locus_tag="VYN16_001781"
     CDS             complement(3661..4149)
                     /locus_tag="VYN16_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV1404278.1"
                     /translation="MSSYFVKITDASKAVKNGDQAEVQKLVTKMASDFERVENKDSEV
                     GKIVKEKLALSGDITEAKLTEISSALLAFEKEQNPVDLDAEKEKLVNRLSPRFETLEQ
                     AIASKDLEKVREAFKKMNSTWTINESVVRDNSTAHYGRVETAISFLPSSMETEPTDES
                     GT"
     gene            complement(4256..4927)
                     /locus_tag="VYN16_001782"
                     /pseudo
     CDS             complement(4256..4927)
                     /locus_tag="VYN16_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(4930..5389)
                     /locus_tag="VYN16_001783"
                     /pseudo
     CDS             complement(4930..5389)
                     /locus_tag="VYN16_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         1689 a         1188 c          978 g         1581 t
ORIGIN      
        1 aattttatct gatgattgcc caaatttttc aataataaaa cgcataatat tatggagaca
       61 atcccctata ttatgcgttc ttttaatatc aaagactttt tgacaaactt ctttgatatc
      121 taattacatg ccccctgcag gaatcgaacc tgcaactact ccttaggagg gagttgttat
      181 atccattgaa ctaagggagc tagataaaaa ctctgctgaa aagcagagtt ttttagtcga
      241 attaacgacg gatttctttg atacgagctg ccttaccttg aagagcacgc aagtagtaca
      301 atttcgcacg acgtacttta ccgtaacgta caacttcgat tttttcaaca cgtggagtgt
      361 ggattgggaa gatacgctca acacctacac cgttagagat tttacgaact gtgtagtttt
      421 cagagatgcc agcaccttta cgtgcaataa caacaccttc aaaaatctgg atacgttcac
      481 ggttaccttc gacaactttc gcatgtacac gaacagtgtc accaggacgg aatgatggga
      541 tatctgtacg aagttgacct tcagtcaagc tttggattaa tggattcatt ttattctcct
      601 atctttgtca atcttgagga atcttcctca gcggataaac tgtatttttg tgcgtccatt
      661 acacacaaga tatagtttac caaatttcta aaaaaaagta aagaaattta tagcaaaatt
      721 cctaaaaaaa tcgcaaccaa accacctatg taagtcaaag tcaggtaaga ataaaagacc
      781 ttcttatcac ttagcagtct ttgaagttcg tcattcaagg tcgaaaaagt tgttaaaccg
      841 ccacaaaatc ctgttgctag aatagcatat acttccttag actctacatg attgtagaat
      901 actccaatta aaaaacaacc taaaagatta gctatgagcg ttccgagtgg caatttagaa
      961 gcttgattat agcgagaaaa gaaataccgt acgagtgctc ccaagccaca ggcgattgca
     1021 agatagacga ttaccatttc ttcctcccca aatagtaagc taaaagcagg cctccaccga
     1081 tactcaaaag caaatacagt atcaaactaa gataacgccc tgtatcaagc agcttcacag
     1141 tatcaagcat taggctggaa aaagttgtta aacctccgca aaaacccgtc cccagtgcta
     1201 aaatcaagcc cttactagtc cccttataga ccagatagcc cttgacaaga taaatcaagc
     1261 agaaaattcc cagatagttg acaaagagag tgccccaagg gaaatctgga ctggctggta
     1321 accaagtgga aactaggtag cggacaagtc cgcccaacat agcagctaga aaaatcccta
     1381 gcggataaaa ttgttctttt ttcatttgat attttgatcc tgataatcac gcgaacgttt
     1441 gagtatgtcc gaaaaagtcg cgacaatagt ctcctgatag cgcttgtcct ctacacgact
     1501 tctaaccttt tcaaaaataa ctgcttctct cttagtatct aaaatcggtt tccctgaagc
     1561 cttcttataa gcgacaactc cttcaactaa gtgcattcgt tcttctaaca gtttgacaat
     1621 ttggtcgtcg atttgatcga tttcttgacg aataatatct aaatccatac agtctcctcc
     1681 tttatttgaa ttattgtatc aaaaagccat caattaggct agtaaaatcc caactctcga
     1741 taagaaaaat gcactgattg attacatcag cgcactttta tgcttatcct actttagcaa
     1801 ccaattcttc tgcgaattgt tccaagcgtt caatatcttc ttcctcggca gaaagatcaa
     1861 ctttaacaca ctctgaacct ttttctgctc ctgttgacac aaagacacgg tcaaagtcat
     1921 caacagcctt acagaattca tcgtagaagg tatctcctga gccgaccact ccgtagattt
     1981 tgccattcaa gttgagatct gctaggtctt cgtagaagtc catcatctca tctggcaatt
     2041 caccatcacc ataagtatag gtcgcaacga tagcgatgtc tgcttccaag aagtctgaag
     2101 cgtcaacagt tgtacattca tcaacatcaa catccaagcc caagtcacgt aatttgtctg
     2161 ctacaatatc tgcaatttct tcggtattac cggtcatact ggcaaataca atttttgcta
     2221 atgccatatc gtcctcctca atttatcttt ctccattata tcacatcttt ttcattttaa
     2281 aaataaaaat tcttgtgcta caatgaaatg agaaagaatt gaggttattt atggagattt
     2341 gccaacaaat tttagaaaaa attaaagaat acgacaccat tatcattcat cgtcatatga
     2401 aaccagaccc tgatgccttg ggaagtcagg tgggattaaa agccttgctg gaacatcatt
     2461 tcccagaaaa aaccatcaaa gccgtcggtt ttgatgaacc aactcttact tggatggctg
     2521 agatggatct tgttgaagat agagcctacc aaggcgcact tgtcatcgtc tgtgatacag
     2581 ctaatactgc tcgtatcgat gataagcgct atagtcaagg tgattttctc attaagattg
     2641 accaccatcc aaatgatgat gtatacggtg acctgtcttg ggtcgatact agttcaagta
     2701 gcgctagcga gatgattacc ctatttgccc aaacaaccca actagccttg gcagatcgcg
     2761 atgctgagtt gctctttgca ggaattgtcg gtgatacagg tcgcttcctc tacccttcta
     2821 ccactgcacg gactcttcgt ctggctgctt atttgagaga acataacttt gactttgcgg
     2881 ctctcactcg caaaatggac actatgagct acaaaattgc taaactgcaa ggctacatct
     2941 acgaccatct ggaagtggat gaaaatggtg ctgctcgcgt tatcctgagt caagaagtct
     3001 tgaaacaata caatgtaacc gatgctgaaa ctgcagccat tgtcggtgca cctggacgca
     3061 ttgacagagt gagtctctgg ggaatttttg tcgaacaggc tgatggccac taccgagttc
     3121 gcttacgcag taaagtccat cctatcaatg aaattgccaa ggagcatgat ggtggaggcc
     3181 accctctagc aagtggtgct aattcctata gcctagaaga aaacgaaatc atctaccaaa
     3241 agttaaaaaa cttgcttaaa aactgataaa atacttgcca aacttttcag aatctgatag
     3301 actagtatgg taacaatcta tggctcgcaa agagaccatg gcagaaagga aatattgcaa
     3361 aatgaaaaaa gatatccatc cagaatatcg cccagttgtc ttcatggaca caactactgg
     3421 ttaccaattc cttagcggtt caacaaaacg ctctaacgaa acagttgagt tcgaaggcga
     3481 aacttaccca ttgatccgtg tggaaatttc atcagactca cacccattct acactggacg
     3541 tcaaaagttc actcaagcag atggacgcgt ggatcgtttc aacaaaaaat acggtctcaa
     3601 ataatgataa gagaacagtt tcggctgttc ttttttgttt cttgaaatca actgctgttt
     3661 tcatgttcca gactcatctg taggttcggt ttccatgcta ctaggcagga aggaaatagc
     3721 tgtttcaaca cgtccataat gagctgtact attgtcacga accacacttt cattgatggt
     3781 ccaagtggaa ttcattttct taaaagcttc tcggactttt tccaaatctt tggaggcaat
     3841 ggcctgctct aaggtttcaa aacgaggact tagacggttt accaattttt ccttttccgc
     3901 atctaggtca acaggatttt gttctttttc aaaagctaat agagctgagg aaatttcggt
     3961 gagtttggct tcagtaatat ctccagatag agctaacttt tccttaacaa tcttaccaac
     4021 ctcagaatcc ttattttcta ctctttcgaa gtctgatgcc atcttcgtaa cgagtttctg
     4081 gacctcagcc tgatcaccat ttttaaccgc tttggatgca tctgtgattt ttacgaaata
     4141 agaactcaag ctttcttggg cacctgctgg ggaaagaatt aagaattaag aattaagaat
     4201 taagaattaa gaattaagaa ttaaaaatga caaagctaaa acaagcaacc agcttttagc
     4261 cttccaataa tttctgacca atgtagcctc ctttctccac accaccaaag caagtaaaaa
     4321 gtccactacc tatgtgagtg atgtattcat tcatcttatc agtagcacct aggttagttt
     4381 gaaccttaac aaagttatca ggatccttct ggaaagaaat gaagagcaaa cccgtatcaa
     4441 actgaccagt cttttcatca atgccatctg agtaagaata agaacgacgt aaaagtggct
     4501 tatccacttc tttggccaaa caaacatgcg aatcatcagg taagagactc aaatccactt
     4561 catcaaactc atttttctta ccaaaggggg caccgcattt ctccccttac ggatgagatt
     4621 gcgaatagcg tgaaaggcaa tttgctcatc atctgcacaa gcatgaatca caatatctcc
     4681 accagtatat ttttcacgta attgctcctt aggaaataga ggtaaatccc tgaacagtcg
     4741 agggcgcttg ttttccaagt tcatcctttt caagaaactt gcagatacac caaaagtcag
     4801 cgtcaaacga tgaggattga gcccaactgt catccgtcca atccttgaat agctggataa
     4861 ttttatctct atccgtcgta tgcaggtcca agacaacaaa atagatattc ttttgcatgg
     4921 gcgtactatt tggtacatca agcgaacctt atgaaaaggt ctactgattt taaagcgctt
     4981 tcttgtcttc ttgaattgag attttatttt tagacgcgac aatggtttga gcgtctgctt
     5041 tcttaggatg ttctggacgt ggcataatat ttcgccaacc atggcgcttc aacagttgat
     5101 agaaggcgtc acgtgtgtag gaacgaccta actccttttt ataagcctga aataaggcat
     5161 caattgtaac aaattctcct gactctgcag ccttcaaatg acgggcaaga aaggctttct
     5221 cttcctcaac tgtcatatat gcatggttac gaccactacg tgtttcttga aggagagagt
     5281 cgagttcgaa ctcctcatat ttttttacat ttcgctaaat cgttgtttga ttacagtcta
     5341 aaagttctat aatctcttta taagatttgc ccatcagacg aaatataata aattgaaact
     5401 agaatagtac acctctactt ctaaaatatt attaga
//
LOCUS       DAVLTM010000113         5217 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLTM010000113 DAVLTM010000000
VERSION     DAVLTM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.114"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            89..466
                     /gene="mscL"
                     /locus_tag="VYN16_001784"
     CDS             89..466
                     /gene="mscL"
                     /locus_tag="VYN16_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1404279.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(508..1602)
                     /gene="hemH"
                     /locus_tag="VYN16_001785"
     CDS             complement(508..1602)
                     /gene="hemH"
                     /locus_tag="VYN16_001785"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1404280.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTIRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            1757..2980
                     /gene="pepT"
                     /locus_tag="VYN16_001786"
     CDS             1757..2980
                     /gene="pepT"
                     /locus_tag="VYN16_001786"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1404281.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLIDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(3016..3645)
                     /locus_tag="VYN16_001787"
     CDS             complement(3016..3645)
                     /locus_tag="VYN16_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404282.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            3610..3831
                     /locus_tag="VYN16_001788"
     CDS             3610..3831
                     /locus_tag="VYN16_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404283.1"
                     /translation="MDLFQELICLVQKTFYFFLSICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(4102..5113)
                     /locus_tag="VYN16_001789"
                     /pseudo
     CDS             complement(4102..5113)
                     /locus_tag="VYN16_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         1516 a         1018 c          936 g         1747 t
ORIGIN      
        1 aaaataatgc tccatttatg agccttttaa ttgccttcta taatgattta ggatacaatg
       61 aaacacgaaa cattgaaagg aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg
      121 aggaaatgtt attgaccttg ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt
      181 tacttcactt gtaaacgaca ttatcactcc tcttatttta aatcccgctt tgaaagctgc
      241 taaagtcgaa cgtatcgctc aactttcttg gcatggagtc ggctatggta actttttaag
      301 tgctattatc aattttatct ttgtgggtac cgccctcttc tttattatca agggcattga
      361 aaaagcacag aaactgactg gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga
      421 attggaagtc cttcaagaaa taaaagctct ccttgagaaa aaataatcga ttaaaaggat
      481 ctagcgcaaa gctaaatcct tttttcttta ctctttgggt aacttgcctg ctttttcaag
      541 catttttttg ataagataag gcatcttaac attctcccgc ccttttttct caatcagttt
      601 tttcacaaat tctggcattt gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc
      661 tgaaggaact aactcatcaa aggtctcttc ttctgggaga aattccttaa actcttgatt
      721 ttcattgact ctaattgtat tgaccaaagc atcaatcaaa cgagaatctg tatttggcat
      781 tggtggacga tggtagttta ccccaaattc ctgacataag tcataacatt ccacatcatt
      841 gtcaaacaag acctcaatat gctcactgat gaaactaata ggtacaaaaa tataatggtc
      901 tggatgttct gtctgttctc tgagatactc caaaacatct ggtttaatcc atggaatacc
      961 aatatcactc tcactctgcc aagtgttggt atattgttct gaactcaagc ccagtttttc
     1021 agcgactagc ttgctatttt cgaaaatttg gtctatataa ggatcaccaa agtccaaggc
     1081 aaaaataggc acactatggg ctgaaaagat gactttaaag ctgtcttgct ttacttcttc
     1141 ttttaaaatc ttagcaattt catctgccca atagtttaag agcgcttctt cctgatacca
     1201 gtccttaata accaaaaatt gaatttgttt gctttctaaa aatttttcat agcccatgac
     1261 agagtagaaa gaataatggg gctccaaaat caagcaaata cactgttcaa ttccgtctgc
     1321 ttccatttga ccgatcacat ctggaataaa gggactggaa aacttattag caaagtagac
     1381 actgtattca ttccccagcc tagcttctac caaggcaacc tcttgacggg taattttttg
     1441 cagaggcgtg cctccaattc gtacataatt atcatagaga gtctgaatct cgtggtcttg
     1501 gggtctcacc ccacgacgaa tatttgtgaa aaaatcagct acaccttcaa aggtaatctc
     1561 ttctggtgaa ccgaaggtca tcattaaaat tgcttttttc atgactgctt ccttgtgatg
     1621 tttttatcaa gtttatttta tcattaattc atcgataagt aaacgctaac atagggacat
     1681 tctcaatctt ctgtcttttg tttttctctt ctttctatga tacaatggaa aaaataaatt
     1741 caaaaggagt ttttttatga cttatccaaa tctcttggac cgcttcttaa cctacgttaa
     1801 ggtcaacacg cgctctgatg aacactctac tactactcca agtacacaga gtcaggttga
     1861 cttcgcaaca aatgtcctaa ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta
     1921 tctaccgaat ggttttgcta ttggaacctt gccagccaac gatccgtctt taacacgtaa
     1981 gattggtttt atatcgcaca tggatactgc tgattttaat gctgaaggag tcaatccaca
     2041 ggtaattgaa aactacgatg gtggtgtgat tgaactaggg aattctggtt tcaaactcga
     2101 tccagctgac ttcaagagtc ttgaaaaata tccaggacaa acgctcatca caacagatgg
     2161 aacaagcttg ctaggtgctg atgacaagtc aggaattgct gaaattatga cagccattga
     2221 atatctaact gctcatcctg aaattaagca ctgtgagatt cgtgttggtt ttggtccaga
     2281 tgaagaaatc ggtgttggtg ccaataaatt tgatgcagaa gattttgatg tggattttgc
     2341 ctacactgtt gatggtggtc cactaggtga acttcagtac gagactttct cagccgctgg
     2401 tgctgaattg catttccaag gccgtaatgt ccaccctggt actgccaaag ggcagatggt
     2461 caatgccctt cagctagcaa ttgattttca taatcaactt ccagaaaatg accgacctga
     2521 gttaactgaa ggttaccaag gtttttacca tctaatagat gtgacaggta gtgttgagga
     2581 ggcgcgtgca agctacatca ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc
     2641 atccatgcaa tctatcgctg ataagatgaa tgaagaactt gggagcaacc gtgtcactct
     2701 caacttgaca gaccagtact acaatatgaa agaagtcatt gaaaaagata tgactccaat
     2761 taccattgct aaagccgtta tggaagatct aggtatcacg cctattatcg aaccaatccg
     2821 gggtggaaca gacggctcta agatttcctt tatgggaatc ccaactccga atatctttgc
     2881 aggtggcgaa aatatgcacg gacgttttga atacgttagc cttcagacta tggaacgtgc
     2941 agttgatacc atcattggca tcgtagctta taaaggctaa aaagacgagg tagctcagct
     3001 acttcgcctt tctttttatt ctactggttt ttcttgattt ccagtagttg tagaagattc
     3061 tgttgtttca ttttctgaag ttgattcagc aggtttagaa tctcttgtat tgcttggttt
     3121 gttttcgtcg ctagcagttt caatgttaga ttctgcagtt gcgtttggtt ggttctcagc
     3181 actggtgtta tcactatttg cttcagcatt tcttgctgga cttgtttctt cacttgcgct
     3241 agcttttgac tggatttgat gattcaaaac tagaatagct tttgtcgatt caagtaaagc
     3301 tgttttgtct ttactcttag cagaaagttg atctaataat gcatccacct tatcaaagtc
     3361 cgcatcagat ccattattac tttctaaata agagtgaagc gacatgagaa tatcgtagag
     3421 tttttgatag agtacaagtg tctgagaatc ttgctcagca ttttcctttt cttgttgaag
     3481 ggcgctagcg atacgagtca agacatcttt tacctgactg tttacttcat ccaagtctgc
     3541 atcagccttg tttgtggcag cttttagatt ttctacttct tctgccaagg attgtctgat
     3601 tccttcttca tggatttgtt ccaagagttg atttgccttg ttcaaaagac tttctacttc
     3661 ttcctttcta tctgtcgcag attattggtt gctatctacc atgtactcct aaaacaggag
     3721 agttataatc caagattaca aggccttaca gaaataagaa atccagataa gacaatgttc
     3781 gtccaagacg ctattcgctt cgcacagcag cacggattca atatacttta attttaaagt
     3841 ttaggtgtca agacctcttt ttagtgtgcc caaaatttag agaagtaatc aatcaactaa
     3901 cttttatttt tttcaaactt tcagtaaact gacctaaagc taactcaatc tgtctttgta
     3961 gatgcttctg ctatcagcta gaagttgatc tacttttgcc aagactgcct tctcatcaaa
     4021 agttccaggt tgatagttgg attgcaggga tggaatcttg tttttcaaag ccgcttcata
     4081 tcccttagtt tgaaccttga tgtagtgatt gtggtcgcca tgaggaatca caaaaccttc
     4141 tgaatcttca cttataattc gattggcatc aaaaccatga ccatcttctt cctcatggtg
     4201 gacatgtggt gacggattac ttaatacaga actagaagaa cttcctacct cttccgtgtt
     4261 agagtgtgat gggggattgt taagagatga cttaggaata tagtgatagt gatccccatg
     4321 tcttactata taagcatcac ctgtatctct gacaatatca ttagggttaa agacatatgt
     4381 ggctgctaat tcacctgccg acaagtcact ctcaggaatg aaatgatagt gaccaccatg
     4441 tggtactata gtagattgaa atagaatatg agcaaattga taaggggatt ttaaagtaat
     4501 ttctaacaat gatttagaaa ctatgatgtg ctattctaaa ttcaactcac tatatataac
     4561 catcatcggt agtataacgt ccctgtaatt ttgctacaga tacttctgca ctagctcctt
     4621 tatcgtcttt accatgttct tgtttttggc gattgatttc atcttttgtt cgtacatttt
     4681 ctgcatgagc ttgatcttta aggtaaacat aatactttcc atctacctta ataatatatc
     4741 ctcccttaac ctaactgacg atatcttgat ctttcggctg atagttgggg gctttcatta
     4801 atagctcttc actaaagagc gcatcaaaag gaactttacc attatagtag tgataatgat
     4861 cgccatgaga agttacataa ccttgatctg taatcttaat aacaatttgt tttgcttgaa
     4921 ttccttcttt ttgactaacc tagtctggag tcaaattttc agtcttctta gtgtctttat
     4981 tactgtttac atatgaaaca cgatttttat ctgtattggc ctgttagcta tgttggttca
     5041 gagcataaac acacagactt aaggaaagga taacaacaga tccagctgct atatatttct
     5101 ttttaaattt cataattacc tcatttctat aattatttat atgatgtctt cattattaaa
     5161 tgattaaata aattaattaa ccaattaatt aactagtaaa tattccacct cttttta
//
LOCUS       DAVLTM010000114         5201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLTM010000114 DAVLTM010000000
VERSION     DAVLTM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.115"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VYN16_001790"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VYN16_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1404284.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VYN16_001791"
     CDS             1317..2021
                     /locus_tag="VYN16_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404285.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VYN16_001792"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VYN16_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1404286.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VYN16_001793"
     CDS             3365..4174
                     /locus_tag="VYN16_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1404287.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VYN16_001794"
     CDS             4265..4507
                     /locus_tag="VYN16_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1404288.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VYN16_001795"
     CDS             4509..4763
                     /locus_tag="VYN16_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1404289.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..>5201
                     /locus_tag="VYN16_001796"
     CDS             4810..>5201
                     /locus_tag="VYN16_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1404290.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENVFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRT"
BASE COUNT         1603 a          875 c         1169 g         1554 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattt ttggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggtta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccagat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacaaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaacaa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgtttcat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaat caatgttttg gaagacccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt tgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggtttttt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgat tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatgt ttttgattta
     5041 ttggctattc tccctttaaa tgctattttc acagtttttc gattagggcg tatctttcgt
     5101 ttagttaaac taacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaattag aaagaaaaat tagtagattc ttacggacaa a
//
LOCUS       DAVLTM010000115         5130 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLTM010000115 DAVLTM010000000
VERSION     DAVLTM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.116"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            65..508
                     /locus_tag="VYN16_001797"
     CDS             65..508
                     /locus_tag="VYN16_001797"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1404291.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            510..1025
                     /locus_tag="VYN16_001798"
     CDS             510..1025
                     /locus_tag="VYN16_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1404292.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATSLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            1039..2400
                     /gene="radA"
                     /locus_tag="VYN16_001799"
     CDS             1039..2400
                     /gene="radA"
                     /locus_tag="VYN16_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1404293.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            2473..2970
                     /locus_tag="VYN16_001800"
     CDS             2473..2970
                     /locus_tag="VYN16_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1404294.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            3186..3809
                     /locus_tag="VYN16_001801"
     CDS             3186..3809
                     /locus_tag="VYN16_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1404295.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            3954..4922
                     /locus_tag="VYN16_001802"
     CDS             3954..4922
                     /locus_tag="VYN16_001802"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1404296.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
BASE COUNT         1393 a          890 c         1261 g         1586 t
ORIGIN      
        1 gtgctttttc cgaataaata agatagaata atctagaata aatgataata gaaaagagaa
       61 aattatgaaa attcgtggtt ttgaattggt ttcgagtttt acagatgaaa atttattgcc
      121 caagcgtgag acagcgcatg cggctggtta cgacttaaag gttgctgtgc gtacagttgt
      181 tgcgccagga gagattgtct tggttccgac aggggttaag gcttatatgc agccgactga
      241 ggttctctac ctctatgatc gttcttcaaa tcctcgtaag aagggcttgg ttttaattaa
      301 ctcagttggg gtcattgatg gggattatta tggaaatcct ggaaatgaag ggcatatttt
      361 tgcgcagatg aagaatatca cagaccaaga ggttgttctt gaagttgggg aacgtattgt
      421 ccaggctgtt tttgctactt tcttaattgc agatggagat gcagctgatg gcgttcgaac
      481 tggtggattt ggatcgacag ggcactagaa tgaagattat ctttgtacgt catggggagc
      541 cagattaccg tgagttagag gagcgttctt atataggatt tgggatagat ttggcaccct
      601 tgtctgagat gggacggcag caagtccaga aattgagcaa aaatccttta ctctcgtcag
      661 ctgaaataat cgtatcttct gcagtcacaa gagctttaga aacggcttcg tatgtggtct
      721 gtgctacgag tcttccttta agagtagagc ctttattaca tgaatggcag gtctataaaa
      781 caggaataga aaactttgaa acagctagaa gactgttttt agaaaacaag ggggagttgc
      841 ttcctaatag tcctattcaa tatgagacag ctacggaaat gaagtctcgg tttctagaat
      901 gtatgtctaa gtatcgagaa catcagactg tggtagttgt tgctcatcga atgctcatgc
      961 gccagtttgt gccaaatgag aagattgatt tttgccaagt gattgagtgt gagttagaga
     1021 tatagaaaga ggtttgtcat cgcaaagaaa aaagcgacat ttgtatgtca aaattgtggg
     1081 tataattccc ctaaatatct gggacgttgc cccaactgtg ggtcttggtc ttcttttgtg
     1141 gaagaggttg aggttgccga ggtcaagaat gcgcgtgtgt ccttgacagg tgagaaaacc
     1201 aagcccatga aactagctga ggtgacttcc atcaatgtca atcgaaccaa gacggagatg
     1261 gaggaattca accgtgtgct tggaggcgga gtggtaccag gaagtctcgt cctcatcggt
     1321 ggggatcctg ggattgggaa atcaactctt ctcctacaag tctcaaccca gttgtcccaa
     1381 gtggggacag ttctctatgt cagtggggag gagtctgccc agcagattaa actacgtgca
     1441 gagcgcttag gtgatattga tagtgagttt tatctctatg cagagaccaa tatgcagagt
     1501 gttcgtgcag aagtggagcg tatccagcca gactttctca ttattgattc catccagacc
     1561 atcatgtctc ctgagatttc aggggtgcag gggtctgttt ctcaggtacg tgaagtgacc
     1621 gctgaactca tgcagttggc caagaccaat aacattgcca tctttatcgt aggtcatgtg
     1681 accaaagaag gaaccttggc tgggcctcgt atgttggagc atatggtgga tacggtgctt
     1741 tactttgaag gggagcgtca ccacaccttt cgtattttga gagcggtcaa aaatcgtttt
     1801 ggttccacta atgagattgg gatttttgag atgcagtcgg gcggcttggt tgaggtactc
     1861 aatccgagtc aagttttcct agaagagcgt ttggatgggg cgactggttc ctccatcgtt
     1921 gtaaccatgg aagggacgcg tccgattttg gcggaggttc aggctttggt aacaccgacc
     1981 atgtttggaa atgccaagcg tactacgaca ggacttgatt ttaaccgtgc tagcttgatt
     2041 atggctgttt tggaaaaacg ggcagggctt ctcttgcaaa atcaggatgc ctatctcaaa
     2101 tctgctggtg gtgttaaatt ggatgaacct gccattgact tggctgttgc agttgctatt
     2161 gcttcgagct acaaagacaa gccaactaat cctcaggaat gttttgtcgg agaactgggc
     2221 ttgacaggag agattcggcg cgtgaatcgt attgagcaac gcatcaacga agctgctaaa
     2281 ctgggcttta ctaagattta tgtacctaag aattccttga caggaatcac tctgcctaag
     2341 gaaattcagg tcattggcgt gacaacgatt caggaagtct tgaaaaaggt ctttgcataa
     2401 tccgtgacaa attctcttaa aaatgataag ataggagaaa tatttgacta tcaaattttc
     2461 aaggagggaa tcgtgtcgta ttttgaacag tttatgcaag ccaatcaggc ttatgttgcc
     2521 ctacatgggc agttaaattt gccacttaaa cctaaaacca gagtagccat tgtgacctgt
     2581 atggactcac gtttgcacgt tgcgcaagct ctaggtttgg cacttgggga tgctcatatc
     2641 ttgcggaatg caggtggtcg agtgactgaa gacatgattc gttcgctagt tatttcccag
     2701 caacaaatgg ggacaagaga gattgtggta ttgcaccata cagactgtgg tgctcagacc
     2761 tttgaaaatg aaccttttca ggagtattta aaagaggaat taggtgtaga tgtgtcagac
     2821 caggacttct tgcccttcca agatatagaa gagagtgtac gcgaggatat gcaactgctt
     2881 atcgagtctc ccctaatacc agacgatgtc attatctctg gtgccattta caatgttgat
     2941 acaggaagta tgacagtcgt agaattataa atacttcatt tagaaagaaa gtgtatgaag
     3001 aaaagcagta ttttatttat ttttatttta ttgctatgta ttggtttaca gtatgaaacc
     3061 atctactata cggacggttc gaggtcaggt gcggaatatg gactaatggg agtttctatc
     3121 tttctagctc tcttttacat gattccggct ctttattttc tcttccgtat tgggaaaaat
     3181 gggaattgcc aaagaaggtt ttgattctgt ctttattggg agggatgttc ctttcaggct
     3241 ggttgtctag ttttgctaat acttatatcc atgatttact gggggttctt ttcccagata
     3301 gtacattttt aaatgccttt gaaagtgcta ttgtggctcc tttggtagaa gaacccttga
     3361 aattattgcc acttgttttt gttttggctt tgattcctgt gcgaaaatta aaatctttgt
     3421 ttttacttgg aattgcttcc ggtttgggat tccaaatgat taaggatatt ggttatattc
     3481 gtacggattt gccagagggc tttgatttta ctatttcgcg aattttagag cgtatcatct
     3541 caggaattgc ctctcactgg actttttcag gtctagctgt agtaggtgtt tacttgcttt
     3601 acagagccta taaaggacag aaggttggca agaaacaggg ccttattttt ctaggtttag
     3661 ccttgggaac tcacttcttg tttaactctc cttttgtgga gttggaaaca gagttgcctt
     3721 tagcgattcc agtggttacg gctattgctc tctatggttt ttatcatgct tattgctttg
     3781 ttgagaaaca caatgagttg atgacctaga atatttttca aaagaatgat gcaagggtac
     3841 aaatatggtg cccttctttt atttttgatt gaaaaatagt gcaaaaagcg ctacaatggt
     3901 agatggaaaa tcttgtgaaa agcacaagtt atacatatat accggaggaa atcatgtctt
     3961 tttctgattt aaagctgttt gccctttctt ctaataaaga attggcagaa cgtgtggcgc
     4021 aggagattgg gatagagttg gggaaatcaa gtgttcgcca attttcagat ggagagattc
     4081 aggtcaacat tgaagaatca atccgtggga aacacgtctt tatcctacaa tcaactagtt
     4141 cgcctgtaaa tgacaatctg cttgaaattt tgattatggt agatgctttg aagcgtgcga
     4201 gtgcagaatc tgtcaatgtt gtcatgcctt actatgggta tgcacgtcag gatagaaagg
     4261 cgagagcgcg tgagccaatc acttcaaaac ttgtcgcaaa tatgcttgaa gtagctggag
     4321 tggatcgttt attgaccatc gacttgcatg ctgcgcaaat tcaaggattc tttgatattc
     4381 ctgtggatca tttgatgggt gctcctctga ttgcagatta ttttgagcgt cgtggtatgg
     4441 ttggttctga ctatgtggtt gtcagcccgg accatggagg ggtgactcgt gctcgtaagt
     4501 tggcagaatt tttgaaaaca tctatcgcta ttattgataa acgtcgtagc gttgataaga
     4561 tgaatactag tgaagttatg aacatcatcg gtaaggttga aggcaagact tgtatcttga
     4621 ttgatgatat gattgatacc gctggaacga tttgtcatgc ggcagatgct cttgcggaag
     4681 ctggtgctgt tgaagtctat gcaagctgta cgcacccagt tctttctggt cctgctacgg
     4741 acaatatcca aaaatcagct attaagaaat tggttgtttt ggataccatc tatcttccag
     4801 aagagcgttt gattgataag attgagcaga tttcaatcgc tcatctccta ggggatgcta
     4861 tcgtacgtat tcatgaaaaa cgaccacttt ctccactttt cgatattgag aaaaaaattt
     4921 aatgaccaag cctgagatga ttctcaggtt ttttcgtctt ttttccgaat aaatagatag
     4981 tagcggtgaa tctagtaaag ctagatttaa aactgtgata taataaaaag agaagaaata
     5041 tgactgtacg tcatcagaag tttcagcgac catcattttt gaacagtgat agcacttgaa
     5101 tcgacgcttt ctaaggagaa ttctagtagg
//
LOCUS       DAVLTM010000116         5049 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLTM010000116 DAVLTM010000000
VERSION     DAVLTM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.117"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(116..1678)
                     /gene="guaA"
                     /locus_tag="VYN16_001803"
     CDS             complement(116..1678)
                     /gene="guaA"
                     /locus_tag="VYN16_001803"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1404297.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1820..2518
                     /locus_tag="VYN16_001804"
     CDS             1820..2518
                     /locus_tag="VYN16_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1404298.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2561..3457)
                     /locus_tag="VYN16_001805"
     CDS             complement(2561..3457)
                     /locus_tag="VYN16_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1404299.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLSYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQANIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3466..4401)
                     /locus_tag="VYN16_001806"
     CDS             complement(3466..4401)
                     /locus_tag="VYN16_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1404300.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYSGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQTFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4398..>5049)
                     /locus_tag="VYN16_001807"
     CDS             complement(4398..>5049)
                     /locus_tag="VYN16_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEV1404301.1"
                     /translation="IETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRTRKVEGRK
                     KLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHAEDDRASL
                     VEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASLDFIPAQGQDLTV
                     DNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLGKDRNNVELWFEEV"
BASE COUNT         1583 a         1102 c          947 g         1417 t
ORIGIN      
        1 aatcgaaaaa tggttaagta aaagtcaaca gttactatag tttgcaaatt atttacagaa
       61 agaacaaaaa atgctctgaa acactgattt caaagcattt tttgttagtt taaaattact
      121 cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg ttaacatgat
      181 ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga attttggcaa
      241 aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg tagtcatacg
      301 tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg aagtattgcc
      361 aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg tctgattcac
      421 gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca agtcctggtc
      481 ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta ccaagagcac
      541 gaacttcatc cttgtaaaga gtattaagtg gttcaatcaa ttcaaactgc atatcttctg
      601 gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta ccagactcga
      661 taacatctgt gtataaagta ccttgagcaa ggaatttcac atctttgagc ttgcttgctt
      721 cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt tcaggatcag
      781 aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg atattcaaac
      841 caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga agaagaccgt
      901 ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga accccaacga
      961 cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg acggtttcac
     1021 gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct ttagccttac
     1081 aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga cgaacttctg
     1141 ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg tatgggcagt
     1201 cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca tcaccatggc
     1261 tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt gtgtgagtta
     1321 gggttgattg accgtattca cgatttccag catcacctgc aggaacaact tttcctccaa
     1381 gtttatgggt caataactgc ataccataac agattcccaa aattggaatt ccgagttcga
     1441 agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga ccacctgata
     1501 gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta tggcttttta
     1561 gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg tactggctac
     1621 catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg ttgctcatct
     1681 ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa gggcgaatta
     1741 ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat agagaaaaga
     1801 tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa atccatgatc agataaaaaa
     1861 ggatattgac gagcaccgtt gggctattgg tgagaggctt cccagtgaaa gagatttagc
     1921 tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat tagtcgaaga
     1981 aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc gagtacaaga
     2041 aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta aagttccctc
     2101 tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg ccaagctagg
     2161 aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg accaagttcc
     2221 cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta aaaaagaaga
     2281 aatcaccagt catttcttcc aaaccttgca gaaacatggc tatcgtatcg gcaaatctca
     2341 acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg aagttgaaaa
     2401 aggacatgct attcttggat tgacacaggt ttcctaccta gaagatggta ctgcttttga
     2461 atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa ataattagat
     2521 taccacgcaa aaactctctg tcacagacaa agagtctttt ttatttttct aagaataaat
     2581 ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga aaatcaatgg
     2641 gataaaattg ttcattcatt attaagaagg caaagaaaac cgccgatatt ggctcaattg
     2701 aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg aaagctgtat
     2761 aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa tcaagactag
     2821 tcgggatatt ggcctgtaga acccctgtaa aaggaagggc gaccaaacct gctataacca
     2881 ttcccacacc aatgaccaag ctactccccc actttttaat caaggctatg ggtaaaatga
     2941 tatatagagc ataagtcaag gcagaaaaga gaccccagaa caggccagca ggtgtcatgg
     3001 ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg gcgaatatga
     3061 tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta taaattaaaa
     3121 ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc tcctgaatag
     3181 cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata agaagagaca
     3241 gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta gcataagaca
     3301 agagcatgag aattccacca gcgattaaaa gacgcaagtt ggtcaagacc agagccgaaa
     3361 ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca ataccagcca
     3421 caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact cttctttgcg
     3481 aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt ccttttcata
     3541 ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca tgataaaggt
     3601 acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta aagaacgttc
     3661 cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat ctgctaaata
     3721 gtgttctaaa aaggtttgta aatcctttat agtcgaaaat aaaccagcac tcccagcatg
     3781 tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac ctctaactgt
     3841 tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag gtttccagac
     3901 ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa taaagcccaa
     3961 cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt tgagatgaaa
     4021 catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa aaggatcaag
     4081 gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac tctcaggtaa
     4141 aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga aggtacaaac
     4201 tgtgccaacc ccaacaacct tactgacact agctaggtca taaactagtc ctgcctcagt
     4261 ctgcaagcca tgctctgggt cactctggcc tgaatagaac tctgtccatt gattgtcctt
     4321 aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt ctattttttt
     4381 aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt tctatcttta
     4441 ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa cttctggcga
     4501 agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc ccaggtaacc
     4561 gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga tgccccaatt
     4621 tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat agaaatctca
     4681 aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc tcctacttct
     4741 actagacttg ctctgtcatc ttccgcatga atcaaaatca aatcatcttc tggtgagaaa
     4801 atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc tgttttagat
     4861 aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc tagttttttt
     4921 cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag cttttcaaga
     4981 cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt cattccaagg
     5041 gtttcaata
//
LOCUS       DAVLTM010000117         5011 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLTM010000117 DAVLTM010000000
VERSION     DAVLTM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.118"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(101..2551)
                     /gene="phtA"
                     /locus_tag="VYN16_001808"
     CDS             complement(101..2551)
                     /gene="phtA"
                     /locus_tag="VYN16_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1404302.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(2805..4538)
                     /gene="ptsP"
                     /locus_tag="VYN16_001809"
     CDS             complement(2805..4538)
                     /gene="ptsP"
                     /locus_tag="VYN16_001809"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1404303.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKVNLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4544..4807)
                     /locus_tag="VYN16_001810"
     CDS             complement(4544..4807)
                     /locus_tag="VYN16_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1404304.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1323 a         1051 c          941 g         1696 t
ORIGIN      
        1 aactagttaa tagtttactc tttttaatat tgcttgtcaa gaattttaca tatatacata
       61 ataaaaatga aagcctcttt atcgagactt tcatttttca ttagtttatt ttttccttac
      121 ttacagatga aggattactt ccttttaaca acgcaagtaa tttttctgct tctgccatga
      181 tactattgtt atccataatt tgaagagtca aattatttcg taaaccagct agagtttctg
      241 ttgcattggc tttcagacta gaatccgtta ctttcgcaag caaaacttct gcttctttga
      301 gttgggcttc tactttttca gtctctactt gagggacttc tggctcagca ggtgtttcct
      361 ctactggctc ttcatccgct ttgaagttct tatttggatc ttcactgtgg tctttcttgc
      421 ctaacacatg ctcactggca ttgccccatc catcattaga atgtggacgt tcgtcagggt
      481 gttctacgta gtacttaatc gtcgcaaaca aatcttccaa ggtatagcca tttggagctt
      541 tgtatgtgtg atcatcaaac caagcaaatt taatattatg gtaatgatcc ttatgaggaa
      601 taatcaaatt accgttttta acctcaactg tatgctcaac catatatgga agtcgaacga
      661 gtggaattcg tttttcccct ttcacacgat tgtaaatagc tgctgcacta tctccagttg
      721 gatttgcttt aacatctgcg tctggagatg gaggtaggat acctttttct ttagtatagg
      781 cttgagctgc aactttttcc ttatcagaaa ggctatcttt tccaatccag tgactatggc
      841 ccatatgagg cgttacatat gcatctcctt catcactgat tatatcatgt tcatcaaaaa
      901 tgtaaccatc tgacgttgta tacttatcag ctaattgagc aatacgaact tcgtcttcag
      961 tatactcaat ttgagaattt ggcttgccaa gtcgctctgg atgggtaatt ggtgctagga
     1021 atgccaataa atcatctacc aatttttctt tattagtcga ttcatcattc aagcgttcta
     1081 ataatttgtc taaggcttgg aaatcagaat tacgaccctt attttcaaac aaggctttat
     1141 gagcctcagt taacagatta tatgctttat cataaaattc ttggtcacga ggagcaacat
     1201 tttctttttt agcagttaaa gtgtgtgaaa cactctcttg ttttgataac ttactttcaa
     1261 gatttttaac agtttcagat ggtaaatctt tcgcaaagac ataacgagag atgccctttt
     1321 cttcgaatac atatccttcc ccaacttttc gtaccagctg actaaccaaa gaagaatttg
     1381 agtctatttt aagatttggt gcaggttgcg ggcctggact aggttccgga gtcggttgtg
     1441 gacttggttg ttctggcctt gaatctggta cccaatggtt tgaacgataa cgaaggggaa
     1501 taatacgagc gattcgttct tccaattcag acatttgaga gtaagggatg aagtggtaat
     1561 gatctccgtg tggcactgca acacctctag ctgttcgact tgtgatttgc gctgggtcga
     1621 aaacaaggcc atcagattct acatgtcgtt gactcaaagg cagtttgtag agctgtttca
     1681 agagactatc aatgtcatta ctttgacttg cttgactgtt agtgttgctg ttgttgcttg
     1741 tgttagtatt tgtagttcct ggattgctta cagaaggtac ccagtttgtt cttgaagtgt
     1801 tatcgctatt ttgtcggcga taggttcttg aatttgacag atttccccga ccagatagga
     1861 aggcttctgc agcagccaac tcgctagctg ataactcatt cttaggaatg taatggtaat
     1921 gatctccatg aggaacgata taagcatcac cagtatcctc tatgatatca gaagcattaa
     1981 agatataacc atcatctgta gtatagcgtc cttgcgaacg tgccaaggca acagcaccat
     2041 cgtttcttgg agtcccacct tcacgatgtt gactatgctc ttgtttttgt cgattgattt
     2101 cctcttttgt acggacgtta tccgcgtggg cagcatcctt aaggtaaaca tagtattttc
     2161 catctacctt gataacatat ccacccttga cctcattaac aatatcctca tcttttagct
     2221 tatagtttgg atctttcatg agtaattctt cactgaaaat cgcatcataa ggaaccttac
     2281 cattgtaata atgatagtgg tcgccatgtg aagtgacata gccttggtct gttatcttga
     2341 tgacgatttg ttcggcattg attccttcac gcttgctaac ctcatcagga gtcaaattct
     2401 ccgttttttg cgtcgcttgt tttccatcta tataggaaac acgattattt tccttaaccg
     2461 ttctagcttg atacagtccc aactcgtaag aacaaacact taaaatcaaa gctgccgcag
     2521 aaccaacaag gtatttctta ttaattttca tcgaaactct ctttctattt gatatttcta
     2581 gttgcaattc atttctaaat tgcagaaact cctctctatt acttaactgg ttaatagttt
     2641 actcctaaat ctccaacttg tcaagaattt tatgaaccat ttaagtattt tttgttctga
     2701 ccactacttt ttcaaggttg gactttctat aagaaagatt ttcttgaaaa ttctttttaa
     2761 aatatcaaaa aaatccctgt aactgagttg cagggacttt tcgattaatc aaaattaacg
     2821 tattcttttt gaagttcaag aacttcttcc attgttgagc attctgtaag ggcacggttt
     2881 gcgtactctt ccatcttagc tgtgtcgagt ttcttcatca agctgcgtgt acgaagtaca
     2941 gatgttgctg acatagagaa ctcatccaag cccattccga caagaagtgg aacagcttgt
     3001 tggtcaccag ccatctcacc acacatacca gcccatttac cttcagcgtg agctgctttg
     3061 atcacattgt taatcaagcg taggattgat gggttgtatg gttggtaaag gtatgaaact
     3121 tgttcgttca tacggtctgc tgccattgta tattggatca agtcgtttgt accaattgag
     3181 aagaagtcaa cttctttagc aaattggtct gcaagcatag ccgctgcagg aatctcgatc
     3241 atgataccaa cttggatgtt atccgcaact gcaacacctt cagcaagaag gtttactttt
     3301 tcttcatcaa agactgcttt cgctgcacgg aattctttca agagcgcaac cattgggaac
     3361 atgatacgca attgaccgtg aacagacgca cgaagaagag cacggatttg tgtgcggaac
     3421 atagcatctc cagtctcaga gatagagata cgaagagcac ggaatccaag gaatgggttc
     3481 atttcgtgag gcatatcgaa gtaaggaagt tccttatctc caccgatatc cattgtacga
     3541 acgacaacag gtttaccgtt cattccttca agaacagcct tgtatgcttc atactgctca
     3601 tcttcagttg ggaagtcttg agaatccatg tacaagaact ctgtacggta aagtccaaca
     3661 gcttctgcac cgttgttgtt aacaccttca acgtcttttg gagtaccaat attggcagcc
     3721 aactcgaagt gtttaccgtc agcagtcact gtttgagcat ctttcaaaag tgcccattca
     3781 gctttttgtt tcgcataggc ttcaccagct gctttaaatt ctgccgcttg ttcatctgtt
     3841 gggttgataa tcacttctcc agtgatcccg ttaacagcaa ggatgtcacc gtctttaacg
     3901 atttcagtga tgttgtttgt acctaataca gcagcaattt caagtgtacg tgccatgata
     3961 gctgagtggc ttgtacgtcc accaatgttg gttacaaaag cttttacaaa gtttttgtcc
     4021 aattgagctg tatctgaagg agtcaagtca tgcgcaatca caatcacttc ttcattgata
     4081 gaagctgggt ttggcaattt tttaccaaga aggtttgcca atacacgttt tgtcacgtcg
     4141 cggatatccg ctgcgcgttc ttgcatgtat gggttgtctt ccatgccttc aaagatagtg
     4201 ataaacatat ctgtaacttc tttcagacct gcttctgcat tcactttctt cgcacggata
     4261 gtttccttga tttggctgat catttctggg tcagcaagaa ccattaagtg agcatcaaaa
     4321 acttgagctg cttcttcacc gagcgtacct actgctttct cgcgaataac agaaagctcg
     4381 tcttgtgatg cctgtagagc ggcatcaagg cgagcttctt ctgcgtttgt atcttcgact
     4441 gtaatagtct caaatgacaa atccggctga acgagtagat atgcttttgc aactgcaaca
     4501 ccgtcagatg ctgcgattcc tttaagcatt tctgtcattt cccttatgcc aatccttctt
     4561 tttccattgt ttctgagatt gcagcgatag cgtcatctgc atctgcacct tcagctgaga
     4621 tagttacgtc agcaccttgg ccaacaccaa gactcataac acccataatt gatttaaggt
     4681 taactgattt acctttgtac tcaagagtga tatctgaagc aaatttgcta gcagtttgta
     4741 ccaacaatgt tgctggacgt gcgtgaatac ctgtttctgc cactacgtgg aaatctttag
     4801 aagccatagt ttgactctcc ttttgttctt tctttttttg agttatatgt gataaccctt
     4861 acaatttagt attataccat cttctaggat ttttttcaag cattttctgg attttccttc
     4921 aatttccttt cagataactt gctgaaaatc ttctatccta gataacaaaa cacaggatat
     4981 agtttcttta aaaacaattt ataggataat c
//
LOCUS       DAVLTM010000118         4983 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLTM010000118 DAVLTM010000000
VERSION     DAVLTM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.119"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..303
                     /locus_tag="VYN16_001811"
     CDS             <1..303
                     /locus_tag="VYN16_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1404305.1"
                     /translation="SFSDSLWWALVTVTTVGYGDIVPVSLLGKWLAVLLMLVGIGTIG
                     MLTSALTNFFVKDNPDEQIKLDKLQDELSSPRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(566..1552)
                     /locus_tag="VYN16_001812"
     CDS             complement(566..1552)
                     /locus_tag="VYN16_001812"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1404306.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1746..4214
                     /gene="gyrA"
                     /locus_tag="VYN16_001813"
     CDS             1746..4214
                     /gene="gyrA"
                     /locus_tag="VYN16_001813"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1404307.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            4214..4957
                     /gene="srtA"
                     /locus_tag="VYN16_001814"
     CDS             4214..4957
                     /gene="srtA"
                     /locus_tag="VYN16_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1404308.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         1548 a          854 c         1113 g         1468 t
ORIGIN      
        1 tccttctcag atagtctttg gtgggctctt gtgacagtaa ctactgttgg ttatggtgat
       61 attgttccag tttccctttt ggggaaatgg ctagcagttt tactgatgct tgtcggtatt
      121 ggaacaatag ggatgttaac aagtgctttg acgaactttt ttgtaaagga taatccagat
      181 gaacagataa aacttgataa actccaagat gaattatcta gtccgagaat attactagag
      241 aaacaatcta aaaagataga agaactacat aaaatgatac aagatttgat tgaaaaaaca
      301 taataaaaat aaagatgaga aaaagtttga actgaggaga aatcaagtaa gttttgacac
      361 aatagaacgc atattatcag gtataaaaac ttgatattat gcgttttatt gtgggaagat
      421 gtataatgga ttgaaataag atatgaacaa atcgattagg aatttaaagc attttataac
      481 aacgttttag agtaacgggg tactatttca tataatacag gaaaaaacaa ctccctgatg
      541 attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctggatttt
      601 tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt
      661 ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca acaacagctg
      721 gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact gaaagtggaa
      781 gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac
      841 cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga
      901 acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat tcttcaaggt
      961 ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca
     1021 tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt gcttgacgga
     1081 aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga
     1141 atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt
     1201 tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta
     1261 caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatc acaacaaggt
     1321 cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa
     1381 gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga
     1441 taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta
     1501 cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa
     1561 acatctcctt aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa
     1621 aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc
     1681 tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt
     1741 tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt
     1801 ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct
     1861 taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg accccagaca
     1921 aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg
     1981 gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca
     2041 tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct gccgctcaac
     2101 gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa
     2161 atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag
     2221 cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca
     2281 atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc
     2341 ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg
     2401 ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg
     2461 tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgt atcgttgtaa
     2521 cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc
     2581 aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc
     2641 gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca
     2701 aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac
     2761 cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg
     2821 ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag
     2881 gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa
     2941 cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc
     3001 aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat
     3061 ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg
     3121 aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata
     3181 aacgccgtac agagttgatg gttggacagg tcttgagtct cgaggatgag gacttgattg
     3241 aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg
     3301 acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg
     3361 atgactttgt tcgtgagtta gtgtcaacta gtacccatga tcatctgctc ttcttcacaa
     3421 acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca
     3481 aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta
     3541 tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta
     3601 ttgtgaagag aaccagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc
     3661 tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata
     3721 ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta
     3781 tgagccgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac acagttgttg
     3841 gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa ggatatggta
     3901 agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag
     3961 ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg
     4021 atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac
     4081 aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag
     4141 tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag
     4201 aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatctgct
     4261 gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca
     4321 aattcgaaat attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa
     4381 agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc
     4441 tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac cagttattgg
     4501 gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt
     4561 taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta
     4621 tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc
     4681 tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata aagtttatac
     4741 ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag ttgatgatag
     4801 agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat
     4861 tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg atgaaatcct
     4921 aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga aatcatggat
     4981 ttc
//
LOCUS       DAVLTM010000119         4719 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLTM010000119 DAVLTM010000000
VERSION     DAVLTM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.120"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(286..1014)
                     /locus_tag="VYN16_001815"
     CDS             complement(286..1014)
                     /locus_tag="VYN16_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1404309.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1172..2581
                     /locus_tag="VYN16_001816"
     CDS             1172..2581
                     /locus_tag="VYN16_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1404310.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2599..3894
                     /locus_tag="VYN16_001817"
     CDS             2599..3894
                     /locus_tag="VYN16_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1404311.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3899..4207
                     /locus_tag="VYN16_001818"
     CDS             3899..4207
                     /locus_tag="VYN16_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1404312.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4204..4512
                     /locus_tag="VYN16_001819"
     CDS             4204..4512
                     /locus_tag="VYN16_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1404313.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1590 a          803 c          880 g         1446 t
ORIGIN      
        1 tgttacaaga aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa acactattta
       61 aaactaaata actataagaa gtagatcagg tgaacaattt cagtacctac tctataagct
      121 atacgaagat atttttcaac atgatggata aaacgatcac tcttttaaag ccatcttatt
      181 tagaaaagag aaaccattta agcatagatg ggaccttctt gccctgtttt aaatttttct
      241 taaactagag tctgtaaaag attggcttca ttttcacatt ttctcctagg aggaatgtcg
      301 taatgcaaaa gaattaaatt caaataaatc atagcgatga cgactaatcg agtactcaaa
      361 cggagttccg ttatccaaat aagccacttg ttctacttca aataccggtt ccgttggaag
      421 gagctgcaag taatgttgct cattttctga actagaagaa gctcgcaaga tttttgtagc
      481 actggcaata tgcaatccta actttccttc aatgtaattg taaatcgatt tttgtaaaat
      541 atcttcagta atacctggaa taacatcggt actcatataa gtttgttcca gaacataagg
      601 tttaccgtca ataatacgca ggcggtaaat attataaact ggactctgca aagcgacctg
      661 tagtttttct gctaaaaatt catttgcaaa ttctaatttg aagtgaataa tcctcgagtc
      721 aattgtagaa tgagcatctt cagatatttt tgtcagtccc cggatatctc tttctggaac
      781 gattaatttt ctttttgagc tgccacgaga gagaacaaag gttccctgcc cacgttttct
      841 aaaaattaag ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca ttcgactaca
      901 ggcaaactgt ttagctaatg tcatttcatc aggcaattgt tcgttcatgg tatatttatt
      961 acaaagaatc ttttcacgaa tctcattgta aataaataaa tattttgtac tcatttaatc
     1021 tggttctctc tataatattc taatagtagt ataacaaagt tcaagaaatg aggcaaacac
     1081 aagatatgta taaacaattt ttaaaaatgt ctagacaaat tttaaaatta tgctatactt
     1141 tatgtataga ataatagaaa ggaggttgag catgaaaaaa aagttagtat ttcctaatct
     1201 gttttggtgg ggggctgctt ctagcggacc tcagacagaa ggtcaatatg gaaaagtaca
     1261 tgaaaatgtg atggactact ggttcaaaac gcatccagaa gattttttcg ataatgtcgg
     1321 acctcttgta gccagtaact tttttcatac ttacaccgaa gatttccact tgatgaagga
     1381 aattggagtt aattctttcc gcacttccat ccaatggagt cgactcatca agaatttaga
     1441 gacaggtgag cctgatccaa aaggtattgc tttctacaat gccatcattg aagaagctaa
     1501 aaagaaccag atggatcttg tgatgaattt acatcatttt gatttaccag tggaacttct
     1561 tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag ttattcgtga agtttgccaa
     1621 gactgctttc acatgctttg gagataaggt tcattactgg acaactttca atgagccaat
     1681 ggtcattcca gaagcaggat acttatatgc tttccattat ccaaatctaa aaggaaaggg
     1741 aaaagaggcc gtacaaatca tctataatct aaaccttgct agtgcaaaag tgattcaact
     1801 atatcgctca ttaggacttg atggaaagat tgggattatt ttaaacttga cacctgctta
     1861 tccaagaagt aattctccag aagacttaga agcaagtcga tttacagatg acttctttaa
     1921 caaagtcttc ttgaatccag ctgttaaagg aactttccca gaaagattgg taaaacagct
     1981 agagagagat ggcgtgttat ggagtcatac cgaaaaagag cttcaactga tgaaatcaaa
     2041 tacggttgat tttcttggag taaactacta ccatccaaaa cgtgttcaag cacaagcaaa
     2101 tcctgaggaa tatcagacgc cctggatgcc agaccaatac ttcaaagagt atgaatggcc
     2161 ggagcgtcgc atgaatccat atcgtggttg ggaaattttt ccgaaagcca tttatgatat
     2221 tgctatgatt gtgaaggaag aatatgataa tattccatgg tttatcagtg aaaacggaat
     2281 gggtgttgaa aacgaagcac ggtttatcgg tgaaaatgga gttatcgatg acgtgtatcg
     2341 tattgaattt tatgaagaac atttaagatg gctacataaa gccattgaag agggaagtca
     2401 ctgttttgga taccacgctt ggaccgcatt tgattgctgg tcttggaata atgcatataa
     2461 gaatcgttac ggatttatct ccgttgattt agaaacgcaa aagagaacca tcaagagctc
     2521 aggaagatgg tatcgcaaag taagtgacaa taacggtttt gaagtagaaa ttgaggagta
     2581 aagagaggag atattcagat gaaaaactca aaatttatag atcaatttgc cacctttgct
     2641 ggtaaactag ggaaccaaat tcatttaaaa accctaagag atgcattcgt aacagtaatg
     2701 ccattatata ttttggcagg tttaatcgtt cttttgaaca acacggtatt taagtggatt
     2761 ttccaagggg atacattaac aagattccaa tattggggaa taacaattgc aaacggtact
     2821 ttaagtattt caggtatgat tattgctgta atggttggtt atttcttagc taaaaacaga
     2881 gatttcgaaa acccgttagc agcatcaatg ctatcattag tttctttaat tgtgatgatg
     2941 ccaaatacag tttctgtagt tcctgacgga gcaaaagatg cggtaaacat ttcaggtgtt
     3001 ctttcattca acaacacagg tacaggcgca atgttcgccg gggttatcgt agcgattatt
     3061 gcaacagaat tattcattga attatcaaac gttaaagctt tacaaatgaa ccttggtgaa
     3121 aatattccac cagctgttag tagatcattt agcgtattac ttccagtcat gaccgtcatc
     3181 tccttatttg gggttgtttc agcattatta ttcaatataa ctggaatgaa cttaatctca
     3241 atcattacaa tctttattca agaaccaatt cgtcatattg gtacaagctt aatcggggtc
     3301 attattattt actctttagg aaatatgtta tggctatttg gtattcacca agcagttatt
     3361 tacagtgcca tcctagaacc attactatta attaacatta ctgaaaacat cactgcagca
     3421 aataatggac aagccattcc acacatcatc aacctatcac aaatacaaac attcgcttta
     3481 atgggtggta gtggatctac attatgttta ttaatagcaa cattcttagt gagtcgcaat
     3541 gctgtctcta aaaacgtggc taaattatct tttggacctg gtatcttcaa tatcaatgaa
     3601 ccagtattat tcggttaccc aatcgtttat aacatttcat tagctattcc atttatcaca
     3661 gttccagtcc ttggtatttt aatcagctac ttagcaacag ttacaggatt catgagtcct
     3721 gcatttatac aagttccttg gactacacca gtattcttaa atgcatggtt agcaacagca
     3781 ggggacgtga gagcagttct agttcaattc atcatctttg cacttggagt tcttctatac
     3841 attccattta tcaaagttaa tgacaaagtt gttgagcaag aaatggaagg ttaagattat
     3901 gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca agtatgcttg ttaaaaaaat
     3961 gcaacaaagt gcgactgaac gtgggattga aatttctatc caagcaaaat caatgacaga
     4021 agctaaaaag aatatccatg aagcagatgt cattctaatt gggccacaaa ttcgttatga
     4081 gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta gataccattg atatgcggga
     4141 ttacggaatg atgaatggag caaaagttct ggaacaagca cttgcatgga taggagaaat
     4201 aagatgacag aagaaatgga atatatctgt tttcagctca ttgccaatag tggagcagcc
     4261 aaatcatcct ttattgaagc catccaacta gctaaggcag gaaatttaaa agaagcgaaa
     4321 attaaagttg aagaagcgga agactcctta gtagaagcgc acaagattca ctctaatctc
     4381 atacaaaaag aagctactgg agaaaaaata ggattctcct tgctatttat gcatgcagaa
     4441 gaccaaatgg cttcaacaga gattattcag ttgctatcta aagaattcat cgatctctat
     4501 caaaataaat aggaacactg tcgttcccat ctgtctttat taatgacggc tacattgtag
     4561 gaacctccaa aagttggaca aaatgattga aaggattgga tcctgcatta cacaagatcc
     4621 aatccttttc ttttactcta gtttttataa tgtgttgaat ttagaatagt ccactgcagt
     4681 ttctaaaaaa ttgctggaaa tagatgttaa gctccaatt
//
LOCUS       DAVLTM010000120         4687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLTM010000120 DAVLTM010000000
VERSION     DAVLTM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.121"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            181..2064
                     /locus_tag="VYN16_001820"
     CDS             181..2064
                     /locus_tag="VYN16_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1404314.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     SQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            2213..3100
                     /locus_tag="VYN16_001821"
     CDS             2213..3100
                     /locus_tag="VYN16_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1404315.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(3420..4037)
                     /locus_tag="VYN16_001822"
     CDS             complement(3420..4037)
                     /locus_tag="VYN16_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1404316.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(4452..4687)
                     /locus_tag="VYN16_001823"
                     /pseudo
     CDS             complement(4452..4687)
                     /locus_tag="VYN16_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1371 a          905 c         1029 g         1382 t
ORIGIN      
        1 aaggaattcc tgatgaaaca gtcaaaaaca atcaatctaa agcttgctca ctaagtggat
       61 agaatatttt ttctgcaaaa cgcttgacat atttttaaaa catgataaaa taatagtcgt
      121 aagggcgaat aaaatcgctt tcaaacaaga acaaaatgtt atataaggag atttttgcaa
      181 atgaacaatc aggaaattgc aaaaaaagtc atcgatgcct tgggcggacg tgaaaatgtc
      241 aatagtgttg cccactgtgc gactcgtcta cgtgtcatgg tcaaagatga agagaaaatc
      301 aataaagaag tgattgagaa cttggaaaaa gttcaaggtg ctttctttaa ctcagggcaa
      361 taccaaatta tctttggtac aggtacagtt aacaaaatgt acgatgaagt tgttgtactt
      421 ggcttaccaa catcatctaa ggatgacatg aaagcagaag ttgctaaaca agggaactgg
      481 ttccaacgtg ctatccgtac tttcggtgat gttttcgttc caatcatccc agttatcgta
      541 gcgacaggtc tctttatggg tgtgcgtggt cttttcaacg ctcttgaaat gccacttcca
      601 ggtgactttg caacttacac acaaatcttg acagatacag ccttcatcat cttgccaggt
      661 ttggttgtgt ggtcaacctt ccgtgtattt ggtggaaatc ctgccgttgg tatcgttctt
      721 ggtatgatgc ttgtctctgg ctcacttcca aacgcttggg cagttgctca aggtggtgaa
      781 gtaacagcga tgaacttctt tggtttcatc cctgttgttg gtttgcaagg ttccgttctt
      841 ccagccttca tcatcggggt tgtcggagct aaatttgaaa aagctgtccg caaggttgtt
      901 ccagatgtca ttgacctctt ggtaacacca ttcgtgacac ttttggtcat gtctatcctt
      961 ggactctttg tcattggacc agttttccac gttgttgaaa actacatcct tattgctaca
     1021 aaagcgattc ttagcatgcc atttggtctt ggtggtttct tgattggtgg ggttcaccaa
     1081 ttgatcgtcg tgtcaggtgt gcaccacatc ttcaacttgc ttgaagtgca attacttgct
     1141 gctgaccatg ctaacccatt caacgctatc atcacagctg ctatgacagc tcaaggtgct
     1201 gctactgttg cagttggtgt taaaacaaaa aatccaaaac tgaaaacact tgctttcccg
     1261 gctgctcttt ctgcctttct aggtattaca gagcctgcta tcttcggggt gaacttgcgc
     1321 ttccgtaaac cattcttcct ttcattgatt gctggtgcaa tcggtggtgg attggcttct
     1381 atccttggac ttgctggtac tggtaatggt atcaccatca tccctggtac aatgctttat
     1441 gttggtaacg gacaacttcc acaatacctt cttatggtag ctgtatcatt tgcccttggt
     1501 tttgctctta cttacatgtt tggttacgaa gatgaagtag acgcaactgc agctgcaaaa
     1561 caagctgaag tggctgaaga aaaagaagaa gttgcgccag cagctcttca aaatgaaaca
     1621 cttgtaactc ctatcgtcgg tgatgttgtc gctcttgctg atgtcaatga cccagtcttc
     1681 tcaagtggag ctatgggaca aggtatcgct gtgaaaccaa gccaaggcgt ggtctatgca
     1741 ccagctgatg ctgaagtttc aattgccttt ccaacagggc acgcttttgg tttgaaaaca
     1801 agaaatggtg ctgaagtttt gattcacgtt ggtattgaca ctgtatctat gaacggtgac
     1861 ggttttgaag caaaagtttc tcaaggtaat aaggtgaaag ctggcgatgt tcttggaaca
     1921 tttgactcaa acaaaatcgc tgcagctgga cttgatgata caacaatggt tatcgttaca
     1981 aatacagctg actacgcttc agtagctcca gtcgcaacag gttcagttgc gaagggggat
     2041 gctgtgatcg aagtgaaaat ctaatcaatc ctctctaatg tgaaaacgaa caaatgacat
     2101 gtttgttcgt ttttattaga tagtaagatt tacagaggaa aatctaaaaa atagagacat
     2161 ttagactttc gaagtatgct ataataaaga aaataaaaac aagaggttta tcatgacaaa
     2221 attatatgga agcttggaag cgggcggtac aaagtttgtc tgtgctgtcg gtgatgaaaa
     2281 ctttaacgtt gtagaaaaaa cacaatttcc aacaacaact ccaatcgaaa caatcgataa
     2341 aaccattgag ttcttctcaa aattcgataa ccttgctggt cttgcagttg gttcatttgg
     2401 gccgattgat attgacaaaa actcaaaaac ttatggcttt atcacgacga ctccaaaacc
     2461 aaactgggca aatgtggact tgcttggtgc ccttcgtcgc gccctaaacg tgccaatgta
     2521 cttcacaaca gacgtaaaca gctctgctta cggtgaaatg gttgcccgta acaatgctgg
     2581 tggtcgtatc gaaaacttgg tttactacac aatcggtaca ggtatcggtg caggcgtcat
     2641 ccaacgtggt gagtttatcg gtggtgtggg tcaccctgaa atgggtcatt attatgttgc
     2701 tagacaccca atggatattg aaaaagagtt taagggtgtt tgtcctttcc ataagggatg
     2761 tctagaaggt tatgcagctg gtccaagttt ggaagctcgt acaggtgtac gtggggaaac
     2821 tattgaactc aacaaccctg tttgggatgt tcaagcctac tatatcgctc aagctgcggt
     2881 taatgcgaca gtgactttcc gcccagacgt gattgtcttt ggtggagggg tcatggctca
     2941 acaacatatg ctggaccgtg tccgtgagaa atttacatct cttcttaatg gttacctacc
     3001 agtaccagat gtgcgtgact atatcgtgac gccagcagtc gcaggaaatg gttctgccac
     3061 acttgggaac tttgttcttg caaaagaagt ttcaaaataa agcacaaaat ccgcttggga
     3121 aaccaaacgg attttgtggt tgaaaacaat atttttgagc ctttccttaa gtgattacgg
     3181 acggtagcga ctttcttcga aattccatac ctaaactttg agcctagtgt ctcaaagttt
     3241 ccgaatacct gaaaccatta gatttcaggt gtttctatca cggcggaaag gctaggtaag
     3301 tcctcgcctc actatcgaaa aatatgcatg atttccatac caaagtatat ttagtatcaa
     3361 gctatagtta atctacgctt taaaaatcca cttgggaaac caaacggatt ttttacgtgt
     3421 caaagcattt gccagaaaaa gtcaataata taggtcagac cgtaggtata aaccacgagg
     3481 gtaaagggtt tggtcagaat gatgatggtc atgtagcgct tgaaactcat cttggtcagg
     3541 gcagccagca tacagagaaa gtcagctggg ctaatgggcc aaatcatcat aaaaataaag
     3601 aagcggtcaa aacgattgcc cttatctagc cagccgatgt acttgtcgta ggtgcgcttg
     3661 ctgacgacag actggacaaa ggcagctccg tataggcgca ctagataaaa gataatggca
     3721 cagccaatca cgatgccgat atagttgtag atagtcccga tgatgtgccc gtagataaag
     3781 accccagcca ccgaggtcaa ggcccctgga atgataggga cgacagtctg taaaatctgt
     3841 aaaaagataa agagaggtgg accccagatg cctgcctgct ggataaaggc agagagggtt
     3901 tccttggatt gtaaaatccc agcctgataa gcccaaatac agaaaatcaa actcccaacc
     3961 ccaccgacaa tcgatgagat attgataact cgctgcagaa ggggagaaac agctttcatt
     4021 tgtttatcct gtgacatgta aactcctcat agatagtata tttctactat accatatttt
     4081 ggaggagaaa gggggaatga gtcttttttg ataaggaata gaaagtaaat agcccttcga
     4141 gacttagaat aggtctgtca actaaaggtt atatgcattc taggtttcga ataatcgcaa
     4201 ttccataaat ttaaaagcat tctaagtttc gaatagctta tctgagataa ttttgaggca
     4261 ttcgcgcttt cgaatagtca tttttcctaa agtttagatg tattctgaag ctagaaagac
     4321 taaaatctaa aagtagtaag agtctctctc tgatggacgt aatttatggc tagtatgaat
     4381 cctttgaatt gttagttaat tttaatatta atcagttctg aaataattac taaatatagt
     4441 tagatggtga attaattatt gctaaatttc aagttcaagt tagtcatcca gaagtcttct
     4501 ctgggggact tgtagttcaa gcatttttta ggatagttgt taatccactt ttcgatgaat
     4561 gcgacttcct ttgtagtcgt tttcttagtt ccctgaggta accatctatg aatgagtctg
     4621 ttgtgatttt cattagatcc tatagtgatg tgcataatag atatagcaga ttgaaactag
     4681 aatagta
//
LOCUS       DAVLTM010000121         4659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLTM010000121 DAVLTM010000000
VERSION     DAVLTM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.122"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..227
                     /locus_tag="VYN16_001824"
     CDS             <1..227
                     /locus_tag="VYN16_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404317.1"
                     /translation="WFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMVNS
                     NPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            237..935
                     /locus_tag="VYN16_001825"
     CDS             237..935
                     /locus_tag="VYN16_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404318.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDFLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            932..1579
                     /locus_tag="VYN16_001826"
     CDS             932..1579
                     /locus_tag="VYN16_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404319.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1666..2532)
                     /locus_tag="VYN16_001827"
     CDS             complement(1666..2532)
                     /locus_tag="VYN16_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1404320.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHLTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(2544..3307)
                     /locus_tag="VYN16_001828"
                     /pseudo
     CDS             complement(2544..3307)
                     /locus_tag="VYN16_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3308..3509)
                     /locus_tag="VYN16_001829"
                     /pseudo
     CDS             complement(3308..3509)
                     /locus_tag="VYN16_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
     gene            complement(3606..3986)
                     /locus_tag="VYN16_001830"
     CDS             complement(3606..3986)
                     /locus_tag="VYN16_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1404321.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(3946..4614)
                     /locus_tag="VYN16_001831"
     CDS             complement(3946..4614)
                     /locus_tag="VYN16_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1404322.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRIPV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVVLPSFSANVIFLIKKTSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFGNPST
                     LSRK"
BASE COUNT         1398 a          790 c         1003 g         1468 t
ORIGIN      
        1 cttggtttaa aaaatcaaac atgagcttag tatttctgtt tactttttta tttgtagaat
       61 ccttattctg gatttatcag ttggacaatg ggataattgg attattgcca atttttcagt
      121 atatggtaaa ttccaatccg tatgcattga tttattggct tacattacta tctatcataa
      181 ttccattgac tgtattttct gttcatagaa actggaggag agtgtaaaag ttggaaatgg
      241 gaaagttaag tagtcacatg tggaggttga atcagataat ctataccaag tacttttggg
      301 gttatgttct tttttggata ttgatttgtt taggattatg gtattggtta gaaggaaatg
      361 atagacttgt tatagaaatt ttaaaagggc ctaatctgag tcaaaactct tttttagtct
      421 tatctatatg gttgcttcat tggtttatta ttcatacatt ttttctagca gttgtatatc
      481 gtagaagagc atccgatttc tttatggaag tgattcgatt ttcttctatt aagctctgga
      541 ttaggtatca gatttggacc tgttttcttt atggactcat tttaatcatg gtaaaagttc
      601 tagtgattca atttatgtta cagttaccaa actgggatat aggagttttg tttatagttg
      661 attttttgaa tgcttgtgtg ttagtcttgt tttgctttat gttatacgca ctaggagcga
      721 atgtacaaat gaactttgct tgcgttagtt tctttttact catgattgtg tttggtggtc
      781 tatttgtagg aaatcgaact aattatctat tttatatttt aaatcgaggg aatggagata
      841 taggacgaga tttatttctt cagttgctat ttttagtctt tctttttaaa agcattttct
      901 atttcactcg tcaaaagagg aggtttatag aatgaatgaa ataattacat taaaaaatat
      961 tcagttggaa ttaaaaaaaa catgtgtttt tcaaaacctt aattttagtt gtaaacaggg
     1021 ggaaattata ggaattactg gtgcgaatgg ctcagggaaa agtgtattgt ttaaattaat
     1081 agctggttta tatagtccgt cttatggaga agtgttaatc aatggggaaa atattgttcc
     1141 tgagagaaaa attccagcta atttgggagc tttgattgaa gaacctggtt ttataaatta
     1201 ttatagtggc tttaagaatt tacaatattt ggcaagcata cgaggagtag ttggtaatca
     1261 ggaaatcaat gatacactga aaatagttgg tctatatgag caaaaagacc agaaagttaa
     1321 aacttattcg ctaggtatga ggaaaaagct agggattgct caagcaatta tggagaatcc
     1381 ctctattctt ttactagatg aacctatgaa tgccttggat aaatcaagtg tagaaaatat
     1441 gagaacattg tttagaaagc tctctagtga aaaagggaca acaattttga ttgctagtca
     1501 tagtgaagag gatattcgta tcttatgtga taaagtatat gcaatagaag ataaagtatg
     1561 tacactgtgt tcagattgaa catcttagat agactcagga tgttataggt aaaatcttaa
     1621 aaagggattg gatcttaaaa tccagttcct tttaagagtt aatgactaat caacttttcc
     1681 accttcaaca accaggtcat ctgctttagc agcgtcaccg taggttaggt gaagtgtttt
     1741 ttcataggcc ttgtggaaga agttttcctt gcctaatttt tcaatatctt tattgatgaa
     1801 gtctagcaat tcttggttgc ctttttgaac tgctgccgca atggtatcgg gatcaccgag
     1861 ggaagtaatt cctacttcaa atcctttatt ttcaagcgcc caagctagaa cttccgtatt
     1921 gtcagttgaa aaggcatctc cacgtccgtc aagaagagct tggtaagagt cactgtattg
     1981 gtcgtatttt tggagtttga tttctggatg attcttttca aaataagtct cagcagtcgt
     2041 tccttttgtg acaattaagg ttttaccttc aagttgtttg acgtctgtaa tgagaccagt
     2101 cttaggtgat acgacaccca gagaaacttt catatatgga agggcaaaat caacttgttt
     2161 cttacgttcg tcagttactg taaagttagc aagagtaata tctaccttgt ttgaaatcaa
     2221 gtattccgca cggttggcag catcgactga aatgtattta accttgacac caaggtcttg
     2281 agctagttgg ttccctagtt caatatcgta gccttggtaa gaaccatcat tgtcaacgta
     2341 gccaaacggt tttttatctc caaacacggc gattcgcagt tcaccgcttt ttttgatttc
     2401 atcgatagtg cgagctttgg cagttgtttt tccagaggat gaaccagcgt ttccacctga
     2461 gctacaagca gtgataaaga taagggcaaa ggcaagtgct aaaacagtta agagtggttt
     2521 gaatagtttc ataagaatct cctttataga tatgagccga attggctaaa gtcaaagacg
     2581 tttaaaaatt cctgggctcg tttggtttgc ggattggtaa agaaggcttg agctgttcct
     2641 tcttcagcga ttttcccttg gtcgaggaag ataatccggt cagcaatggc ttgggcaaac
     2701 tgcatttcgt gggttactaa aatcatggta cggccttctt gggccaaatc attgataagt
     2761 tccagcacct cacgcaccat ttctggatcc agcgaagcag tcacctcgtc aaaaaggatg
     2821 atttctggat gcattaggag ggcacggaca attgcaacac gttgcttctg tccaccagat
     2881 aattgacggg caaagcaaag ctatgttgtt tatccagtaa accgacacga tctagtaatt
     2941 gcaaagcttc ttccgttact tctttcttgt cccttccttg agctttgata gggcctagga
     3001 tgaggttttg taagacatcc agatggggaa agagttcata actttgaaag accatgccaa
     3061 tcttttggcg aactaggtga aaatcttttt tattttcaac gatagactga ccatccagaa
     3121 gaatatctcc accttgaata ctttctaagc cgttgaggca acgaaggagg gtacttttcc
     3181 cacaaccaaa tggccctagg atgacaacaa cttccccttt tttgatttct agagaaagtc
     3241 cttggaggat gggattgtct ccgaaggatt tttttagttc cttgatttct aagatagttt
     3301 cagacattta gtttctccaa tgtttttcta agtgagtgga tagtttggaa atagggtagc
     3361 aaactgcgaa atataagact agaatggttc cataaatcca aaatgaagca gttggggatg
     3421 gtcaggcgat tgctatcgat gatttgttgt ccaactttgg tcacttccac aaccccaatc
     3481 aaaacaacta atgaagtggt tttaatcatc cgagtgacaa gattgatagc ctgcggtagc
     3541 agtcttctta agacttgtgg gatgatgatg tggtagtaaa gttgaacatt agtcaagccg
     3601 agtgcctatc cactttcaaa ctgatgttta gggagagaag tgatagctcc acgtaccaag
     3661 tctcccattt cagctgttcc ccagagggta aaaatgataa tagctgaagt ctcacctgag
     3721 atattgatat taaagtttcg agccaagcca aagtaaacga tgaagagtag caccagctgg
     3781 ggcatgatac ggataaattc cagatacaat cgtgttaaaa atcgtatgat tctagaatgg
     3841 gaggtcatga tgattcccat gactgttccg aacatcatgg ataagaggac agacaggatg
     3901 gatatcccaa tcgtaacgcc caacccctgt aagattctca ggagattatt tccttgaaag
     3961 agtacttgga ttcccgaatc ctgcatggcg ggtcctcctt tctatccagc taaagaccag
     4021 tgagatgggt agcagcatga tcagataagc aactaccaac atagctagcg caatgtctgt
     4081 ctcatagtag agaccaatca aatccttggc gacgtacatg aggtcggcca aagccactgc
     4141 tgagaaaaca gaggttttct tgataaggaa aatgacattg gcactaaagg aggggagtac
     4201 caccgctgtt gcttgcggaa gaaccacata gcgaaagacc tgtagaggtg tcagaccaat
     4261 agcgaggcca atctcctgct gggtttgact gatggcttcc agcccacttc ggaaagattc
     4321 tgccatatag gagcctccta aaaagacaag tccaagcgtt gcacagactt ctgaagatag
     4381 gacaatcccg attcggggaa gaccgaagta gagaaagaag agttgaatca aaaggggcgt
     4441 attacgtgac aattcaatgt aggctgtcgc tacttgcgct aaaacaggga ttcgataatg
     4501 tcggatgata ctaacgatta aaccgagcag aaaggatccc aaaattcccc aaactgcaat
     4561 atgcaaggtc agaaagaatg ccttttgata tagtggtaga tattgttcaa caatggatca
     4621 atccaaaaat agaacctccc atctagaaat aatacagtt
//
LOCUS       DAVLTM010000122         4653 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLTM010000122 DAVLTM010000000
VERSION     DAVLTM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.123"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..785
                     /locus_tag="VYN16_001832"
                     /pseudo
     CDS             1..785
                     /locus_tag="VYN16_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(818..1158)
                     /gene="tnpB"
                     /locus_tag="VYN16_001833"
                     /pseudo
     CDS             complement(818..1158)
                     /gene="tnpB"
                     /locus_tag="VYN16_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1374..2724)
                     /locus_tag="VYN16_001834"
                     /pseudo
     CDS             complement(1374..2724)
                     /locus_tag="VYN16_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2860..3159)
                     /locus_tag="VYN16_001835"
     CDS             complement(2860..3159)
                     /locus_tag="VYN16_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1404323.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(3324..3775)
                     /locus_tag="VYN16_001836"
                     /pseudo
     CDS             complement(3324..3775)
                     /locus_tag="VYN16_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            3857..4653
                     /locus_tag="VYN16_001837"
                     /pseudo
     CDS             3857..4653
                     /locus_tag="VYN16_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1371 a          873 c          871 g         1538 t
ORIGIN      
        1 atgacgattg agcaagagaa agcctttctt gcccgtcatt tgaagactac agatgcagga
       61 gaatttgtta caattgatgc cttatttcag gcttataaaa aggagttagg tcgttcctac
      121 acacgtgatg ccttctatca actgttgaag cgccatggtt ggcgaaatat tatgccacgt
      181 ccagaacatc ctaggaaagc agacgctcaa accattgtcg cgtctaaaaa taaaatctca
      241 attcaagaag aaaagaaagc gatttaaaac cagtagacgt tttcgtaagg ttcgcttgat
      301 gtaccaagat gaggctggtt tcggtagaat cagtaaactg ggatcttgtt gggctccaat
      361 aggagtaggt ccacatgtcc atagtcacta tatacgagaa tttcgctatt gttatgccta
      421 tacaggcgaa tcatttttct taatagctgg tggatgtaat actgagtgga tgaacgcctt
      481 tttagaagag ctttcacaag cttatccttt tactcgttat ggacaatgct atatggcata
      541 aatcaagtac cttaaagatt ccgactaata ttggctttgc atttattcct ccgtacacac
      601 cagagatgaa ccccattgaa caagtgtgga aagagattcg taaacgtgga tttaaaaata
      661 aagcctttca aactttggaa gatgtcatga atcaacttta agatatcata caaggactgg
      721 agaaggaggt gataaagccc atcgttaatc ggagatggac tagaatgctt tttgaaagca
      781 gatgagtata aaaagaaagt cctcatttca atagaaatca cgactttctg atggatttat
      841 ttttggagtg atagaaaagc ccttcataag ccagtctact tgttcaggtg tgagagcttt
      901 gacatccttt tctgtactgg gccaagtcag tctgccgttc tcaaagcgtt tatatagtag
      961 ccaaaatcct tgaccatccc agtaaagggc tttaaagcgg tctttacgtc caccacaaaa
     1021 gagaaagact tgaccggaga aaggatccaa ttcaaagtgg gttttaacta cataggctaa
     1081 tgagtctatt ccctgcctca tatctatctt gctacaaaca aggtgaactt gacctaaatc
     1141 acttagttga attatcatag tacaatacct ttcctccgat aattattttt tatctagtat
     1201 actggaagtt ggggaattag gatagatacc ttgttatgac gcgcttacgt aacttgtaac
     1261 tagctgccta gtttgatctt tgcttcttca ttgattagca gtagatttca aaatgataaa
     1321 aacgtataat agtaggtgta gtgtactgcc ccccaaaaag ttagacaatt aatttatccg
     1381 aaggatttag ttctgtattg cacagggcta agtcctttta gttttacctt aattcgttta
     1441 ttgttgtagt aatcaatata gtctataatg gcttgttcca attggttaag tgatttaaat
     1501 gttttctcat agccataaaa catttcggat tttaaaatgc caaagaaaga ttccatcata
     1561 ccgttgtctt tgctgtttcc cttgcgtgac atagatgctt gaattccctt actctctagg
     1621 aaccgatgat aagaatcgtg ttgatattgc cagccttggt cactatggag aatcgtattc
     1681 tcgtagtgct tctctgtgaa tgcctgttcc aacattgttt gtacttgttc taagttgggt
     1741 gaagttgaaa gattataggc gataatttcg ctattaaagc catctaaaac tggtgataag
     1801 taaagctttt gagtacttac tggaatggca aattcagtca catctgtgta gcactgttcc
     1861 attgttttag agccttcaaa ttggccttga atgagattct ctgctttctt gccaacgtct
     1921 cctttatgag aagaatattt tcgtttctgt cgcgttttag cttgtaaatt gagtacttca
     1981 tcaagtgttg aactctttta tgatttacca gataaccacg atttcttagt tctaaataaa
     2041 tccgacgata accatagttg cctttatgtt cattataaat ggcctgaatt taggttttaa
     2101 tctctttatc tttatcaacc ccatccagtt gtttcaactg ataatagtaa gtcgagcgag
     2161 ctaaacgtgc tgtttcaaga agaaaatcta gtcgaaatcc tcctgaaacc atttctctaa
     2221 ctgtctctgc ctttctcgct ctagggcttc gtccctttcc tctaactctt ttaacttttt
     2281 taggtaggcc acctcagtcc gtaagcgttc attctcctcc tggagtcgtt ctagttctgt
     2341 catttcttcc caagtcttct tccgtttacg tcccatttta gctggtctcc ctcttgtttt
     2401 ctcaacaata gtatacccgt ttttcttgta ttgtgccagc caaaatgaaa gcattccttg
     2461 gtttgggagg gcataatcaa gactaacact tctttgtgaa caaccttcca gtaaggcttt
     2521 atccatcatt tcttgtttca atttagaaga atagtgacga ttcttcccct ttttaacgat
     2581 ttctatgcca tgacggtcaa ttaatttcac catgtacttt agaccagaaa catccacacc
     2641 aaatcttttt gaaagctgtt tgaagctttg tccttgcttt cttagttcat agatctgaac
     2701 tttatcttca taacttaatt tcataataaa acaccccaaa agttagattt tttctgtcta
     2761 acttttaggg ggcagttcat ttcggcacct gatactatgc gttttgttat ggaaagactt
     2821 attggacttt ctctcaaatc gagtttttac tcaattttct tacttgattg ggattgaaat
     2881 tccaattaat ttctctgagt agagtgtctt gatattggct tcatcaacag aggtcttatc
     2941 aattttacgt ttcaagaaaa attcttgaat ggtttcgatt tcaggctcac gaatagcacg
     3001 gtgtttgttt gagatgagga tttcatagtg aagcggagct tgggtaaaaa taacatctgt
     3061 attccctgca gaataaacct caacaagggt tgcatcggta ctttctagtt gactttttac
     3121 aagttgcgag tgtgagtttg tcgtattgat aagcttcata atatttcctc cgattttcta
     3181 attctattat aacacttttt gaataaagtc gcttgattta tactcaatga aaatcaaaga
     3241 gcaaactagg aagctagccg caggctatac ttgagtacgg taaggcgacg cttacgtgat
     3301 ttgaatttta ttttcgaaga gtattagcca atcttatgct gttttttcca agattcaatg
     3361 gcccatttat ggctgccacg tttaaggttt ttgatagcct cgtcaatagg gaaccaggca
     3421 atatgattaa agttttctag tggcttttgt acttctttga aaggagttgc ttcatagagg
     3481 taggcaggat tgtagtagta ggtatcacga tgacgagaat agaaatattc gtcagctggt
     3541 ccgtaatagg taccaatttc tgctgtgaaa ccaagctctt caatcaactc acgctttagg
     3601 gcttcctgat gattttcacc tgcttcaatt tttccacatg gtaggaacca agcaccattt
     3661 ggttcttgaa caagaacaat ttgtttttgt tcaggattag ggataactgc atatacgcca
     3721 tagcgagcaa tatagtctgt attcactttt tttctccgaa agttgggttt gccattgcat
     3781 tttcctcatt atctagtatc gttattatta tagtgaaatg aaccaaaaat agtacacaat
     3841 gtggtataat cttcttatgg catattcaat agattttcgt aaaaaagttc tctcttattg
     3901 tgagcgaaca ggtagtataa cagaagcatc acacgttttc caaatctcac gtaataccat
     3961 ttatggctgg ttaaagctaa aagagaaaac aggagagcta aaccaccaag taaaaggaac
     4021 aaaaccaaga aaagttgata gagatagact taaaaactat cttactgaca atccagacgc
     4081 ttatttgact gaaatagctt ctgaatttgg ctgtcatcca actaccatcc actatgcgct
     4141 caaagctatg ggttacactc gaaaaaaaga accacaccta ctatgaacaa gagccagaaa
     4201 aagtagcctt atttcttaag aattttaata gattaaagca cctagcacct gtttagattg
     4261 acgaaacagg attcgatact tatttttatc gagaatatgg tcgctcatta aaaggtcagt
     4321 taataagagg taaagtatct ggaagaagat atcagaggat ttctttggtt gcaggtctaa
     4381 caaatggtga gttaatcgct ccaatgactt acgaagagat gatgacgagc gacttttttg
     4441 aagcttggtt tcagaagttt ttcttaccaa cattaaccac accatcggtt attattatgg
     4501 ataatgtaag attccataga atggggaagc tagaactttt atgcgaagag tttgggcata
     4561 aacttttacc tcttcctccc tactcgcctg agtacaatcc tattgagaaa acatgggctc
     4621 atatcaaaaa gcacctcaaa aaggtattac caa
//
LOCUS       DAVLTM010000123         4595 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLTM010000123 DAVLTM010000000
VERSION     DAVLTM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.124"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            8..694
                     /locus_tag="VYN16_001838"
     CDS             8..694
                     /locus_tag="VYN16_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1404324.1"
                     /translation="MRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLVAGQT
                     GNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIPLAIL
                     SIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDY
                     FRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLSMLFA
                     SWQKKVKEKA"
     misc_feature    759..846
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            882..1196
                     /gene="rplU"
                     /locus_tag="VYN16_001839"
     CDS             882..1196
                     /gene="rplU"
                     /locus_tag="VYN16_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1404325.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1212..1556
                     /locus_tag="VYN16_001840"
     CDS             1212..1556
                     /locus_tag="VYN16_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1404326.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1573..1866
                     /gene="rpmA"
                     /locus_tag="VYN16_001841"
     CDS             1573..1866
                     /gene="rpmA"
                     /locus_tag="VYN16_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1404327.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2071..2276)
                     /locus_tag="VYN16_001842"
                     /pseudo
     CDS             complement(2071..2276)
                     /locus_tag="VYN16_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2714..3631
                     /locus_tag="VYN16_001843"
     CDS             2714..3631
                     /locus_tag="VYN16_001843"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1404328.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3724..4572)
                     /locus_tag="VYN16_001844"
     CDS             complement(3724..4572)
                     /locus_tag="VYN16_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1404329.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1452 a          784 c          946 g         1413 t
ORIGIN      
        1 agggaaaatg aggttattac ctataagaaa aatatcacgt cagtctaaaa ggttagcact
       61 ttttttgacg ttttgtgctg gatatgtgga tgcttacact tttattgttc gcgggaatac
      121 ccttgtagct ggacaaactg gaaatgttgt ctttctttca gtagaattaa ttaaaaataa
      181 tgtttcggat gttagggaca aggttctcac cttgctagcg tttatgatgg gagtcttttt
      241 attaacgatt tataaggaaa aattgagaat tgtgaaaaaa cctattctgt ccttgattcc
      301 cttggcaatc ttatcaatca ttattgcttt tgtgccgcaa actgtggata atatctatct
      361 agtgccgccc ttggccttct gtatgggact ggtgacaact gcttttggag aagtgtcggg
      421 tattgcctat aataacgctt ttatgacagg gaatatcaaa cggaccatgc tggcttttgg
      481 agattatttc cgaaccaagc acactccttt tttgcgtgaa ggattcatat ttgttagcct
      541 gcttagtagt tttgtccttg gcgttgtctt ttcagcctat ttgacgattt tctatcatga
      601 aaagaccatt cttggtgttc ctattatgat gagcgttttt tacctcagca tgctttttgc
      661 ctcttggcag aaaaaagtaa aagaaaaagc ttgattttag gtgttattac ttgtaagaaa
      721 ataggagata tgctatactt gaagagttga ctatgcacga tcctgtgcaa ccgcacgaac
      781 atcgttgctt gattattcaa gtttaagtgg ttcgtcccac gatgctaggc gagtcttcac
      841 aaaaaatcgg ggaaacccat aaaaatcata ggaggtgcat aatgagcaca tacgcaatta
      901 tcaaaactgg cggaaaacaa gttaaagttg aagttggtca agcagtttac gttgaaaaat
      961 tgaacgttga agctggtcaa gaagttactt ttaacgaagt tgttcttgtt ggtggtgaaa
     1021 atactgttgt cggaactcca cttgttgctg gagctactgt agttggaact gttgaaaaac
     1081 aaggaaaaca aaagaaagtg gttacttaca agtacaaacc taaaaaaggt agccaccgta
     1141 aacaaggtca ccgtcaacca tatacaaaag ttgtcatcaa cgcaatcaac gcttaatttt
     1201 aaggagaaca catgatacag gcagtctttg agagagccga agatggcgag ctgaggagtg
     1261 cggaaattac tggacacgcc gagagtggcg aatacggctt agatgtcgtg tgtgcatcgg
     1321 tttctacgct tgccattaac tttatcaatt ctattgagaa atttgcaggc tatgaaccaa
     1381 tcctagaatt aaacgaagat gaaggtggct atctgatggt tgaaatacca aaagatcttc
     1441 cttcacacca gagagaaatg acccagttat tctttgaatc atttttctta ggtatggcaa
     1501 acttatcgga gaactattct gagttcgtcc aaaccagagt tatcacagaa aactaacacg
     1561 gaggaaaaca ttatgttaaa aatgactctt aacaacttgc aacttttcgc ccacaaaaaa
     1621 ggtggaggtt ctacatcaaa cggacgtgat tcacaagcaa aacgtcttgg agctaaagca
     1681 gctgacggac aaactgtaac aggtggatca atcctttacc gtcaacgtgg tacacacatc
     1741 tatccaggtg taaacgttgg tcgtggtgga gacgatactt tgttcgctaa agttgaaggc
     1801 gtagtacgct ttgaacgtaa aggacgcgat aaaaaacaag tttctgttta cccaatcgct
     1861 aaataaaaag gtccattgaa ccttttatcc cgaaccttga aatgtaaagg tgaggaagct
     1921 agaaacagct taaaataagg ctttccgagt tttcggagag cctgtttttt tgttttggta
     1981 cccatacttt ttaatatgta tagtggattg aatctggaat agaacactgt taaaaattga
     2041 tatgagtttc ctactcgatt tcttcagatt ttatttcaat tgactataag aactgctatc
     2101 tggtttcaaa tattggcttg tcgtcccttc acttgtctga atggtttgag ccgttccttc
     2161 tgataaggga aaagatattc tttagctgaa actgtctgag aatccaagct aacagatgga
     2221 gagaaaaata gcccagtctg aagcacacta gatgggagtt gttggaaagc agtcatctag
     2281 tttttctttg ctgttttggc ttttttcttt gtcatcaggg aaagtgactt gcttcaaatc
     2341 agggagttta ggaaattctt caaacatctt gcccaagttg tctcattttg taaattttac
     2401 agccataggt gtacctcgat tctaaattct accttttcgg caccaattag taatttaaag
     2461 ttctcaggag ttagttgcat tgctttttca tacataagca tagtatgaca ctttatggtt
     2521 accgaacaag tctaatcact ccttgcaaaa ttttgaagat gtcctacaag tttttgagaa
     2581 gaatattgca aagttcatag ttaagcaacg atagaataaa gtgctttttg aaaacaggta
     2641 cacttcatgt gaaaaaaaca acatttcaga ttttctgttc cagaaatagc tatcgctatg
     2701 atataattat tttatgatta ttactattcc tataaaaaac caaaaagata ttggcacacc
     2761 atctgattca gtcgttgttc tcggctattt tgatggcata cataaggggc atcaagaatt
     2821 atttcgtgtt gccaacaagg ctgcgagaaa ggatttatta cctatcgtcg ttatgacctt
     2881 taatgaatct ccaaagatcg ctttagagcc ttatcatcca gatttgtttt tgcatatttt
     2941 gaaccctgct gaacgtgaaa gaaaattaaa gcgcgaaggt gtagaagaat tatatctcct
     3001 tgattttagt agtcaattcg ctagtctcac tgcacaagaa ttttttgcaa cttatatcaa
     3061 ggctatgaat gccaaaatta ttgttgcagg ttttgattat acatttggtt ctgacaaaaa
     3121 aacagcagaa gatttaaagg attactttga tggagaagtt atcattgttc cacctgtaga
     3181 agatgagaaa ggaaagatta gttcaactcg tatccgtcaa gctattttag atggaaatgt
     3241 gaaagaagca ggaaaacttt tgggggcacc gcttccatca agaggtatgg tggttcatgg
     3301 taatgctcgt ggtcgtacaa ttggttatcc gacagcgaat ttagtgcttt tagaccgtac
     3361 ttatatgcca gcagatggcg tttatgtcgt tgatgttgag attcaaagac agaagtatcg
     3421 tgctatggct agtgtcggga aaaatgtgac ctttgatgga gaagaagcac gttttgaagt
     3481 caatattttt gattttaatc aagatattta tggggaaacc gtcatggttt attggcttga
     3541 tcgcattcgt gatatgacca aatttgactc agttgaccaa ttagtggatc agttaaaggc
     3601 tgatgaagaa gtaactcgga attggtctta agagcttgag taaataaaat aaaaaagagg
     3661 ttgtctgtaa cccaaaagat agatgattta gtctaacttt tgaggtcacg acattacctc
     3721 tttttattct ttttcaaagg tgaagccttc tcctaggatt tcatgggctt ctgtaatagt
     3781 tataaaggct tgaggatcga ttcgatgaat catttccttc gttttcacaa tttcatttct
     3841 tccgacaata cagtagatga ttttcaaatt ttctttacta tagtagcctt gaccagaaat
     3901 aaaagtaaca cctcttccga ggtcatcatt aatcgcctta gcaagttggt caggacgttt
     3961 tgtgataatc ataaagcctt tgccggcata tcctccttca ccaatcaaat caataacacg
     4021 agaaacaata aaatcaaaca aaagcgtgta ggaaaccaat ctcaaatcct tgaagattag
     4081 gagaatcaac atgagaatac aaaaatctaa gataaagagc agttttccta tggatatatg
     4141 agtgtatttg ttgagaatac gagctagaat atcagttccg ccagttgtac ctccagcatt
     4201 aaaaataatt ccaaggccaa ttcccaatag gattcccgct ataagggctg tgattagtaa
     4261 atcaccttga agatcaatat gaaggggaat atgctcaaaa aaagctaacc agccggacaa
     4321 agctaaggtt cctagtaaac tagaatagag ggatttggct ccaaagatct tccaagctag
     4381 gatgaaaagg ggaatattaa tcagcaggtt catgagggaa acagggattt taaaaagata
     4441 aaatgtgatg agggtaatac ctgtcgcccc tccttcaaag agatgatggg gaactacaaa
     4501 ataagtcagt ccaaaagcat aaatagcagc acctagtaaa atggtaaaaa tggggtaaat
     4561 ttttttaatc ataggacacc tcttttctta taagt
//
LOCUS       DAVLTM010000124         4495 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLTM010000124 DAVLTM010000000
VERSION     DAVLTM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.125"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            153..1319
                     /locus_tag="VYN16_001845"
     CDS             153..1319
                     /locus_tag="VYN16_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1404330.1"
                     /translation="MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVK
                     QKANQEKIAFQKAGSTRQKAVTIKNYQELATLWLESYKNTVKPNTQGNVKKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHITKTLNRDIEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRERVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
     gene            1480..2424
                     /locus_tag="VYN16_001846"
     CDS             1480..2424
                     /locus_tag="VYN16_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1404331.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            2436..3119
                     /locus_tag="VYN16_001847"
     CDS             2436..3119
                     /locus_tag="VYN16_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1404332.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPNMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            3374..4405
                     /locus_tag="VYN16_001848"
     CDS             3374..4405
                     /locus_tag="VYN16_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1404333.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLRLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDAYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
BASE COUNT         1451 a          949 c          843 g         1252 t
ORIGIN      
        1 ctgaatttca aaaatataac cacattaatg ctatgtattc tttactggaa gaaataaaac
       61 gaaaataagg gggttctcgt agctcctagc atgatataaa acctttttga taatggcttg
      121 cctgctgatg tattttagaa aggtttatca tcatgaaaat aactgaagtt ataaaaaaag
      181 acggtagcaa agtttatcgt gctaatgtat atctaggagt tgaccaggta acaggaaaga
      241 aagtaaaaac caaagtaaca ggtcggacac aaaaggaagt taagcagaaa gccaatcaag
      301 aaaaaatagc ttttcaaaaa gcaggatcta ccagacaaaa ggctgttacc ataaaaaact
      361 atcaagaatt ggcaactctc tggttggaaa gttataagaa tacggttaag ccaaacactc
      421 aaggcaatgt taaaaagcta atcgataatc atatattgcc tatttttggg gcttacaagc
      481 tcgataagct cactactcca cttatccagt caattatcaa cgaacttgct gacaagacta
      541 atagagggga aaaagcggct tttctacatt atgacaagat acacgcttta aacaaacgta
      601 tcttacagta tggcgtaacc atgcaggcta taccgtccaa tcctgcgcgt gatgttgttt
      661 tacctcgtaa cactcagaaa gcaaagcgga aaaaagtaaa gcactttgaa aatcaagaac
      721 taaaaaaatt tcttggttat ctcgataatt tagatactca tagatatcgt tactactatg
      781 agacaacact ctataagttc ctattggcta ctggttgccg tatcaatgaa gctttagctc
      841 tttcctggtc tgatattgac ttagataatg ccgttgtgca tattaccaaa acactaaatc
      901 gggatataga aatcaatagc ccaaagtcta aggctagtta tcgggatata gatatagatc
      961 aagcgactgt tagcatgctg aagcagtaca aactacgcca aactaaagag gcctggaaga
     1021 tagggcaacg tgaacgcgtg gtattttctg atttcattca tgagtatcct agcagttcaa
     1081 gacttaaaag aagattgcaa acacatttta agcgtgctga tgttcctaac attggatttc
     1141 acggattccg tcacactcac gctagtctct tgcttaactc tggaatacca tacaaggaac
     1201 tccagtaccg cctaggtcat tccactttat caatgaccat ggatatatat agtcacctct
     1261 caaaagagaa cgcaaaaaaa gcagtatcat tctacgaaac tgcactaaaa gcactatagg
     1321 gggagcaaaa aggggagcaa atttaaaaat caacatttca agacaaagta aaaatccaat
     1381 aacaacggtg tctttgtaag attttatttg aaaaaagccg attttgtagt ataatagtac
     1441 agtgtgaaaa aatctgaaaa atgagaaagg ataagggata tgaaacaagt ttttctctct
     1501 acaacaactg aatttaaaga gatcgatacg cttgaaccgg gtacttggat caatctcgtc
     1561 aatccgactc aaaatgaatc actcgaaatc gccaacacct tcgatattga tattgccgac
     1621 cttcgagcac cgctcgatgc ggaagaaatg tctcgtatta ccattgaaga cgagtatacc
     1681 ctgattatcg tagacgtgcc ggtcacggag gaaagaaata accgcaccta ctacgtaacc
     1741 atcccgcttg gtattatcat cactgaggaa accattatca ctacgtgttt ggaaccacta
     1801 cctgtccttg atgtctttat caaccgtcga ttgcgtaatt tctatacctt catgcgttca
     1861 cgttttatct ttcaaattct ttatcgcaat gcagagcttt acctaacagc ccttcgttca
     1921 atcgaccgca agagtgaaca aatcgaaagt caactgcatc aatcaactcg taatgaagaa
     1981 ttgattgagc tcatggaatt ggaaaaaact atcgtctatt tcaaggcctc cctcaaaaca
     2041 aatgagcgcg tgattaagaa attgaccagt tcaaccagca atatcaagaa ataccttgag
     2101 gacgaagacc tgcttgaaga caccctgatt gaaacccaac aggccatcga gatggcagat
     2161 atttatggaa acgtcttgca ttctatgaca gagacctttg cctctatcat ttctaacaac
     2221 cagaacaaca tcatgaaaac cttggccctt gtgaccatcg tcatgtccat cccaaccatg
     2281 gtcttttctg cctacgggat gaactttaag gataatgaaa tccccctaaa cggagagcca
     2341 aatgccttct ggttaatcgt ctttatcgcc tttgctatga gtgtctcgct cactctctat
     2401 ctcatccata aaaaatggtt ctaagaggag ttcctatgtc tcaaattgat ctacaaaaat
     2461 taactaagaa aaaccaagag tttgtccaca ttgctaccca acaattcatc aaagatggga
     2521 aaacagacgc tgaaatccag actatttttg aggaagtcat tccccaaatc cttgaggagc
     2581 aatctaaagg tacaactgcc cgttccctat acggcgcacc aactcattgg gctcatagct
     2641 tcactgtcaa agagcagtac gaaaaagagc atccaaaaga aaatgatgat ccaaaactga
     2701 tgattatgga ctcagctctt ttcatcacta gcctctttgc ccttgtcagc gccctcacaa
     2761 ccttctttgc ggcagatcaa gctttcggct atggattgat tactcttcta ttagttggac
     2821 tggttggtgg atttgccttc tacttgatgt actactttgt ttaccaatac tatggaccaa
     2881 atatggatcg cagtcaacgt ccacctttct ggaaatctgt actagttatc ctagcttcta
     2941 tgttcctttg gttgcttgtc ttctttgcaa caagcttcct accagctagc cttaacccag
     3001 tactggatcc attgccacta gctattattg gagcagccct cctagccctt cgcttctatc
     3061 tcaagaaacg cttgaatatc cgtagtgcaa gtgcaggacc aacacgctat caagaataag
     3121 aaaacgataa aagcaactgc aggtgcggtt gctttttcac ttactttttt gagttatact
     3181 caatgaaaat caaagagcaa actaggaagc tagctgcagg ttgctcaaag cacagctttg
     3241 aggttgcaaa taaaactgac gtggtttgaa gagattttcg aagagtatta aaagtattct
     3301 tctgaaatcc cacatagctt tctcttatat tttgtgataa aataggctca atctatttct
     3361 aggaggatga gatatggttt ctactattgg tattgttagt ttatctagtg gcattatcgg
     3421 agaggatttt gtcaaacacg aagtggactt gggtatccaa cgtctcaagg atctgagact
     3481 caatcccatc tttttgcccc attcgttaaa aggattagac tttatcaagg accatcctga
     3541 agctcgtgca gaggatttga ttcatgcctt ttctgatgat agcatcgaca tgatcctatg
     3601 tgccatcggt ggagacgatg cctatcgctt gctaccttat ctttttgaaa atgaccaact
     3661 ccaaaaggtt atcaaacaaa aaatttttct tggcttctcg gatacaacca tgaaccacct
     3721 catgttgcat aaattaggaa tcaagacttt ttatggtcaa tcctttttag cagacatttg
     3781 tgaattagac aaagaaatgc tagcctatag ccttcactac tttaaagaat tgattgagac
     3841 gggaagaatt tcagaaatcc gccctagtga cgtttggtat gaggaacgaa ctgattttag
     3901 tcccacggcc ctgggaacac ctcgtgtcag tcatacaaat acaggttttg acttgttgca
     3961 aggaagtgcc cagttcgagg ggaaaatcct cggtggttgc ctcgaatccc tctacgatat
     4021 ctttgacaac tctcgatacg cagatagcac ggaactctgc caaaaataca aacttttccc
     4081 tgacttgtca gactgggaag ggaaaatcct cttgctcgaa acaagcgaag aaaagcctaa
     4141 gccagaagac ttcaaaaaga tgttgctaac tttaaaggac actggcatat tcgcggtcat
     4201 caatggactc ttggtcggaa aacctatgga tgaaactttc catgacgact ataaagaggc
     4261 actattggat atcattgaca gcaatatccc gattgtctat aatctgaatg tcggccacgc
     4321 aactccaaga gcaattgtac cctttggggt ccatgctcat gtagatgcac aggaacaagt
     4381 cattctcttt gactataaca aataaagctg ggaaaagttt ctcagctttt ttctcggtat
     4441 cattactggc attttcatca catattacag tatattgaaa ctagaatagt acacc
//
LOCUS       DAVLTM010000125         4465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLTM010000125 DAVLTM010000000
VERSION     DAVLTM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.126"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(88..828)
                     /locus_tag="VYN16_001849"
     CDS             complement(88..828)
                     /locus_tag="VYN16_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1404334.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(828..2393)
                     /locus_tag="VYN16_001850"
     CDS             complement(828..2393)
                     /locus_tag="VYN16_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1404335.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKVIYS
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCNIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            2531..4420
                     /locus_tag="VYN16_001851"
     CDS             2531..4420
                     /locus_tag="VYN16_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1404336.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT         1208 a          826 c         1049 g         1382 t
ORIGIN      
        1 ttgaccttca agtagtagag atagagaatc tgccttttca ttttgaggac agcaaaaaga
       61 ctgcacggtt gatgcagcct tttcttttta tttgagatag cgttgaagga actcttttgt
      121 tcggtcttct ttaggattgg tgaagaggtc ttctggttta ccttcttcag cgatcacgcc
      181 cttatccata aagataacac ggtgagagac atcacgggca aattccattt catgggttac
      241 gacaatcatg gtcaagcctt cctgagccag gtcctgcatg attttgagga cttctccaac
      301 catttctgga tcgagagctg atgttggttc atcaaagaga atagcgtccg gattcatgga
      361 gagggcacga gcgatggcca cacgttgttt ttgaccacct gagagttgtt ttggtttggc
      421 ttgccagtag cgttctccca tgccgacctt ttccaggttt tctttggcaa tcttttcagc
      481 ttctgtgcgt tcgcgtttta ggacagttgt ctgagcgacg attgtgtttt caagaacatt
      541 gagattttca aagaggttaa aggattggaa aaccatcccc aacttttcac ggtattgcgt
      601 gaggtcatag cctttttcga ggacgttttg tccatgataa aggatttgtc catcagttgg
      661 tgtttcaagt aggttaatgg agcgtaggaa ggtcgatttt ccgcttccag agcttccgat
      721 gatagagatg acctctccct tgtggacagt gagtgaaatg tcttttagca cttcgttttg
      781 tccataggat tttttgaggt gtttaatttc aaggattgct tgtgtcatta tttcaaatcc
      841 tccgtttgca tttggttagc acctgtagtg taggtatcca tgtccattct gcgctcgata
      901 aagcgtagga tacgtgttac ggtgaaggtg aggacaaagt aaatcacggc gatgattgta
      961 aatgtctgga agtattgata ggtttgtgtt gccacggtat ttcctgagaa ataaagctcg
     1021 acaacagaga taacgttcaa tacagatgta tctttgatat tgatgacaaa ttcattacca
     1081 gttgctggta ggatattgcg gacaacctga ggtaggacaa tattacacat ggtttggtta
     1141 tgagtcatac caagagcagt cgcagcttca aattgtccct tgtcaactgc caggatacca
     1201 ccacggacga tttcagtcat gtaggcaccg gtattgattg aaacgatgaa gatagcagcc
     1261 agtgtacggt caaggttgat accgaaagct tgggcagttc catagtagat aaccatcgat
     1321 tgaacaatca ttggcgtacc acggaaaatt tcaatgtaga cgttgaggat ccagccgact
     1381 agtttttgca ggctgtaaat gactttgttt tcagagagag gagcagtacg gaaaacacca
     1441 atggcaagtc caataatgag acctgtgatg gttccgacga tagagattaa aagagtgata
     1501 ccagcaccac gcaagagttg ttgccagttt tcagaaagaa ttttagcgac ttggctaaag
     1561 aaactactgc tagtctcttc agttgttgta gcttcggcag gttgttcctt gatcatacga
     1621 tccatcaagg caacttggtc atcttttgaa atggtttcaa tgctggcatt gatttggcta
     1681 atacgattgt catttttacg aagcccgata gcgatagctg tatcttcttc cccagttttg
     1741 aaaccaggtt ctacttgaat catcttgaac ttagagttcg cagcttcagc agtcagtgct
     1801 tctggacgtt cagaaacata agcatcaatg acaccagcct caagagcttg gcgcatttga
     1861 gcgaagtctc ccatggctgt ttctttttta gcacctggga tttgtgcaat caagtcgtag
     1921 aggtagaccc cttgttgaga agtgattttt gcaccgttaa agtcatccaa agatttagca
     1981 cttgcgtagg cagaatcttt tttgacaagc aaaactggtt cgctagtgta gtaactgctc
     2041 gaaaaggcaa tttcttgttt gcgttctgca gttggactca tacctgcgat aatcatgtca
     2101 atcttaccag aagtaagggc agggactaga ccttcccact tggttttaac aaccaaaggt
     2161 tctttaccta agtccttagc gattttcttg gcgatttgaa catcgtatcc gttggcatac
     2221 tgattggtcc catcgatttt gacagctccg ttgctatcat catcctgggt ccagttaaag
     2281 ggagcatatg ctgcttccat cccgatgcgt aaatattcat cggcttgagc aacattgaca
     2341 agtcctagca tcagcaagag acttgtgaaa atagataagt atatttttct catgatttct
     2401 cctattctga tctattaaaa aataactgtc tcctatttta tcgaaaaatg cgtaattttt
     2461 caacataagt aagcctttac ttacgaaaaa atgctataat gataagaaag ataaaaaggg
     2521 ggcttagttg atgaaaaaaa cttttttctt actggtgtta ggcttgtttt gccttcttcc
     2581 actctctgtt tttgccattg atttcaagat aaactcttat caaggggatt tgtatattca
     2641 tgcagataat acggcagagt ttagacagaa gatagtttac cagtttgagg aggactttaa
     2701 gggacaaatc gtgggacttg gacgtgctgg taagatgcct agcgggtttg acattgaccc
     2761 tcatccaaag gttcaggccg cgaaaaacgg tgcagaacta gcagatgtga ctagcgaagt
     2821 aacagaagaa gcggatggtt atactgtgag agtctataat ccaggtcagg agggcgacat
     2881 agttgaagtt gacctcgtct ggaacttaaa aaatttactt ttcctttatg atgatatcgc
     2941 tgaattaaat tggcaacctc tgacagatag ttcagagtct attgaaaagt ttgaatttca
     3001 tgtaagggga gacaaggggg ctgaaaaact ctttttccat acagggaaac tttttagaga
     3061 gggaacgatt gaaaagagta accttgatta tactatccgt ttagacaatc ttccggctaa
     3121 gcgtggagtt gagttgcatg cctactggcc tcggaccgat tttgctagcg ctagggatca
     3181 gggattgaaa gggaatcgtt tagaagagtt taataagata gaagactcga ttgttagaga
     3241 aaaagatcag agtaaacaac ttgttacttg ggtcctccct tcgatccttt ctatctcctt
     3301 gttattgagt gtctgcttct attttattta tagaagaaag accactcctt cagtcaaata
     3361 tgccaaaaat catcgtctct atgaaccacc aatggaatta gagcctatgg ttttatcaga
     3421 ggctgtctac tcgacctcct tggaggaagt gagtccctta gtcaaggggg ctggcaaatt
     3481 cacttttgac caacttattc aagctacctt gctagatgtg atggaccgtg ggaatgtttc
     3541 tattatttca gaaggagatg cagttggctt gagattggtg aaagaagatg gtttgtcaag
     3601 atttgagaga gactgtctaa atctagcctt ttcaggcaaa aaagaagcca ctctttccaa
     3661 tttgtttgcg gattacaagg tatctgacag cctttatcgc ggagctaaag ctgctgatga
     3721 gaaacggatt caagcaagag ggattcagct caaatcttct ttcgaagcag tattaaaaga
     3781 gatgcaagaa ggagtgagaa agcgagttgt cttctgggga ctcccagatt actatcgtcc
     3841 tttaactggt ttggaaaagg ctttgcaagt gggtatgggt gtcttgacta tcttgcccct
     3901 atttatcgga tttggtttgt tcttgtacag tttagacgtt catggctatc tttacctccc
     3961 tttgccaata cttggttttc tagggttagt tttgtctgtt ttctattatt ggaagcttcg
     4021 actagataat cgtgatggtg ttctaaatga agcgggagct gaggtctact atctctggac
     4081 cagttttgaa aatatgttac gtgagattgc acgactggat aaggctgaat tggaaagtat
     4141 tgttgtttgg aatcgtctct tggtctatgc aaccttattt ggctatgcgg acaaggttag
     4201 tcatttgatg aaggttcatc agattcaagt tgaaaatcca gatatcaatc tctatgtagc
     4261 ttatggctgg cacagtatgt tttatcattc aagcgcgcaa atgagccatt atgctagtgt
     4321 cgcaaataca gcaagtacct actctgtatc ttctggaagt ggaagttctg gtggtggctt
     4381 ctctggaggc ggaggtggcg gcagtatcgg agccttttaa agagagctac catagactga
     4441 aaaagtatga tataatggaa gatag
//
LOCUS       DAVLTM010000126         4457 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLTM010000126 DAVLTM010000000
VERSION     DAVLTM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.127"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            2..2182
                     /locus_tag="VYN16_001852"
     CDS             2..2182
                     /locus_tag="VYN16_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1404337.1"
                     /translation="MVLSAFVANSVAAQETETSEVSTPKLVQPVAPTTPISEVQPTSD
                     NSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVTKELKDKFTSGDFTV
                     VIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEARDTSSNKNNLVSRP
                     ASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNFLNIKDIKGIDYYML
                     GGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHLIYTANDTTGSNYFR
                     IPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTWTKPKLTLAFDDFAP
                     VPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPAGVSFREATRKDSGY
                     KQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDKNFGIKQNGNYLTVE
                     QYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISSNDHGESWSAPTLLP
                     PIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDNGASWKVKVVPLPSS
                     WSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYLKFVSNPSYGTQLSI
                     INYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRYHHDVDYSNYGYSYS
                     TLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN"
     gene            2222..3331
                     /locus_tag="VYN16_001853"
     CDS             2222..3331
                     /locus_tag="VYN16_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1404338.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3488..4378
                     /locus_tag="VYN16_001854"
     CDS             3488..4378
                     /locus_tag="VYN16_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1404339.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
BASE COUNT         1528 a          654 c          927 g         1348 t
ORIGIN      
        1 agtggtatta tcagcatttg ttgctaactc agttgcagct caggagactg aaacttctga
       61 agtatcaaca ccaaagttgg tgcaacctgt tgcaccaacg actccgattt cggaagtaca
      121 acctacatcg gataactctt cggaagttac tgtacaacct cgaacagttg aaactactgt
      181 taaggatcca tcttctacag cggaagaaac tcctgtctta gaaaaaaata atgttacttt
      241 aacagggggc ggagaaaatg ttactaaaga gttaaaggat aaatttacta gcggtgactt
      301 tactgtagtg attaagtaca atcagtcaag tgagaaaggc ttacaagctc tgtttggaat
      361 atctaattcc aaacccggtc aacaaaatag ttatgtagat gtgttcctta gagacaatgg
      421 tgagttgggg atggaagcgc gtgatacttc ttccaataaa aataacctag tatccagacc
      481 tgcttcagtt tggggtaagt acaaacaaga ggctgtgact aacactgttg cagtagtagc
      541 agattcagtc aaaaaaacat attctttata cgcaaatggt acaaaagtag tagaaaagaa
      601 agtggataat ttcctaaaca tcaaggatat taaaggtatt gattactata tgcttggggg
      661 agtgaaacgt gcaggaaaaa cggcgtttgg ttttaacgga acactagaaa atatcaaatt
      721 ctttaatagt gcattggatg aagaaactgt taaaaagatg acaacaaacg ctgttactgg
      781 acatttaatt tatacggcta atgatacaac aggttctaac tatttccgta ttccagttct
      841 gtatactttt agcaatggtc gggtattttc aagcattgac gctcgttacg gtggaactca
      901 tgatttcttg aataaaatta atattgctac aagttatagt gatgataatg gtaagacatg
      961 gactaaacca aaattaacat tggcattcga tgattttgcg ccagtaccat tagaatggcc
     1021 tcgtgaagtt ggtggacgtg acttacaaat cagcggtggt gcaacctata ttgactctgt
     1081 tattgttgaa aaaaagaaca aacaagtact catgtttgct gatgtgatgc ctgctggagt
     1141 aagttttaga gaagcaacta gaaaagattc aggttataaa caaattgatg gtaattatta
     1201 ccttaaatta aggaaacaag gtgatactga ttacaattat actattcgtg agaatggtac
     1261 tgtatacgac gatcgtacca acagaccaac tgaattttca gtagataaaa atttcggtat
     1321 taaacaaaat ggtaattatt tgacggtaga gcaatattcg gtttcatttg aaaataataa
     1381 aaagactgaa tatcgtaatg ggactaaagt ccatatgaat atcttctaca aagatgcatt
     1441 gtttaaagta gtgccaacga actatattgc atatatttct agcaatgatc atggcgaatc
     1501 ttggtctgca ccaactttat tacctcctat aatgggactt aatcggaatg cgccatattt
     1561 aggtcctgga cgtggaatca ttgaaagctc aactggacgt attcttattc cgtcttacac
     1621 tggtaaagag tctgcgttca tttatagtga cgataatgga gcatcttgga aagttaaagt
     1681 agtgccactt ccttctagtt ggtcagcaga agcacaattt gtagaattga gtccaggagt
     1741 aattcaagca tatatgcgta caaataatgg taaaattgca tatttaacaa gtaaagacgc
     1801 aggtactact tggagtgcac cggaatattt gaaatttgtt tcaaatccaa gttatggaac
     1861 acaattatca atcatcaatt atagccaatt gattgatggt aaaaaggctg tcattttaag
     1921 tactccaaac tccacaaatg gtcgtaaaca cggacaaatt tggattggtc taattaatga
     1981 tgataataca attgattggc gttatcatca cgacgttgat tatagtaact atggatactc
     2041 atattcaaca ttgacagagt taccaaatca tgaaattgga ttgatgtttg aaaaatttga
     2101 ttcatggtct cgtaatgaac ttcatatgaa aaatgttgta ccatatataa catttaagat
     2161 tgaagatctg aaaaagaatt aaagctgaaa tttgaaaata tataaaaaga ggataaaaat
     2221 tatggtaaat tacggtattg ttggagctgg atattttgga gctgatttag ctcgctcaat
     2281 gaacaaaatt gaagatgcaa aagtggttgc ggtatttgac ccaaatcatg gagaagaagt
     2341 tgctcaagag ttgggatcag atgtttgtgc aagtttagat gaacttgtag cacgtgaaga
     2401 tattgattgt gtgatcgtag cttcacctag ctaccttcac cgtgaaccag ttgtgaaagc
     2461 tgctcaacat ggcaaacacg tattttgtga aaagccaatt gcattgtctt atgaagattg
     2521 taaagccatg gttgacgcat gtaaagaaaa taatgtcatc tttatggctg gtcacatcat
     2581 gaacttcttt aacggtgtac accatgctaa agaattgatt actcaaggta aaatcggtaa
     2641 agttctttat tgccatgctg ctcgtacagg ttgggaagaa caacaaccaa ctgtatcatg
     2701 gaagaaactt cgttctcaat ctggaggaca tttgtaccac catattcatg aattagattg
     2761 cattcagttt atcatgggag gacttcctga aaaagcgaca atggtaggag gcaatgtata
     2821 tcataaaggt gaaaactttg gtgatgaaga tgatatgctc attgtaaact tagaatactc
     2881 tgatgatcgt tatgctgttt tggaatatgg taatgctttc cgttggggtg aacactacgt
     2941 cttgattcaa ggaactgaag gagctatcaa acttgacttg ttcaatactg gcggtactct
     3001 tcgtgttaaa ggtgaaggag aatcacactt cttagttcat gaaactcaag aggaagatga
     3061 tgatcgtaca gctatctata ccggtcgtgg tatggatgga gcaattgcgt acggtaaacc
     3121 aggagtacgt tgcccattat ggttgcaaac atgtattgat aaagaaatgg aatatctaca
     3181 tgacatcatt aaaggtggag aaattacaga agaatttgaa aaacttctca atggtgtagc
     3241 tgctttagaa tcaatcgcta ccgctgatgc atgtacttta tcagttaaag aagatcgaaa
     3301 agtaagtctt tcagaaatca caaatgctta acttttgtaa aacagaatag taaattcttg
     3361 tcattatata atttctaaag ttctgtgata caactcattg aataaagaaa tagagatggg
     3421 actgggataa tgcccagtcc cattttttat caaaaagtaa tgagatcaaa aatgtgggag
     3481 tgttgaaatg aagattatag gtatcgatat tggcggaaca acaattaagg cagatttata
     3541 cgatgagttt ggaacgagtt tgaatcattt caaagagata gaaacaatta ttgactatga
     3601 tttgggaacg aatcagatat taaatcaggt ctgtgattta attggtgagt atactttaaa
     3661 tcattcaatt gatggtgttg ggatttccac tgctggagtt gttaatgcta atactggaga
     3721 aatcatctat gcaggctata caataccagg gtatatcgga gtaaacttta ctgccgaaat
     3781 agaaaaacgt tttgggttgt atacttttgt tgaaaatgat gttaattgtg ctgcattagg
     3841 tgaattgtgg aagggacaag ccaaagataa gaaaaatgta gtaatggtta ctattggaac
     3901 aggtatagga ggcagtatta ttgtcaacgg acaaattgtt aacggattta actatactgc
     3961 tggtgaagta ggttatattc ctgtaggtaa ttcggattgg caaagtaaag cctcaacaac
     4021 cgcattgatt catttatatc aaaaaaagag cttgaaaact aatcaaactg gacgtacttt
     4081 cttcactgat ttaagatctg gagataaagt tgctgaagaa acttttgaaa tttttgtaga
     4141 aaatctaaca aaaggtttat taacgatttc ttatctactt aatccagaaa ttctcatatt
     4201 aggaggtggg attctggata gtaaggatat tttgttacct gaaattcaaa gttctttagc
     4261 taaaaatgca atggataata ggtttttacc taaaaatctt gtggcagcta cattaggaaa
     4321 tgaagctggt cgtataggag ctgtaaaaaa tttcttagat agaatttcta ataaatagta
     4381 tgtaagataa ggaggtgtca caatgactaa ctctgtattt tcgacaatgc aagatattga
     4441 gaatgttgca accgata
//
LOCUS       DAVLTM010000127         4388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLTM010000127 DAVLTM010000000
VERSION     DAVLTM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.128"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            68..1336
                     /locus_tag="VYN16_001855"
     CDS             68..1336
                     /locus_tag="VYN16_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1404340.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLEDCE"
     gene            1333..2229
                     /locus_tag="VYN16_001856"
     CDS             1333..2229
                     /locus_tag="VYN16_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1404341.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            2244..2561
                     /locus_tag="VYN16_001857"
     CDS             2244..2561
                     /locus_tag="VYN16_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1404342.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            2578..2895
                     /locus_tag="VYN16_001858"
     CDS             2578..2895
                     /locus_tag="VYN16_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1404343.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2905..4260
                     /locus_tag="VYN16_001859"
     CDS             2905..4260
                     /locus_tag="VYN16_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1404344.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMVDKTEL
                     S"
BASE COUNT         1286 a          621 c          984 g         1497 t
ORIGIN      
        1 cttgacgtca aaaaataaag ataattgata acttttgata ataaaatata tttaagagga
       61 aaaggttatg gaaaaattat tacaagaaaa gttacttcca gtagcggcta gattgggtaa
      121 taataaagcg ttggtttcta ttcgagacgg tattactcta actatacctt tattgttgat
      181 aggttcatta ctaatggtta ttgcaagttt tcctattcca ggatgggaaa aatatctggg
      241 tgatattggt gttgctgact atttatggaa gggtgttgat agtagttttg gattacttgg
      301 tcttgtagct agttttggta tcgcttactt tatggctaga caatacaaag ttgatggtat
      361 tcctgctggt attgtgtcat tatcatcttt tattacagtt acgcctttca ttagaggaga
      421 ggctggagca ggaatgccta ccgctttcat ggcatcaaaa ggtttatttg ttgctatgat
      481 tttaggattg attaatggct atatctacca atggtttata aatcataata tacagataaa
      541 aatgccggat ggtgttccac cagcagtatc taaaagcttt agtgccatta ttcctggtgc
      601 agtgactatt gttggttggt tgattgttta tgcaacttta gataaattaa gtttacctaa
      661 tcttcatgaa attgctcaag tagctttggg aggtccactt ggacttttag gaaataatgt
      721 tattggcctt cttatcttaa ttttccttaa tagtagtttt tggtttgtag gattacacgg
      781 aggaaatgtt gttaatgcag ttatgaaacc gttatggtta gctaatctag atgcaaataa
      841 agtagcttat caaactggag aaactcttcc aaatattttt actagcgtat ttatggataa
      901 ttttgtattc ataggtggcg gtggtgcaac cataggttta gttcttgcgt taggatattt
      961 agcacataag aagaaagcta gtaaacaact aaaaactttg gcacctataa ctgttatacc
     1021 tggattattt aatataaatg aaccagcaat gtttggcgtt cctattgttt tgaacatttt
     1081 gttactagtt ccatttatct tagctccaat gtttaactta ctagtagcgt ggggagcaat
     1141 ggcatcaggt ttggttccgc taacttatac agatccaggt tggactatgc cacctgttat
     1201 aagtggttta cttgctacag gaagtatttc tggttcatta ttacaaattg tattgattgt
     1261 tttggatgtc ttactttatt taccatttgt aatagctatt gaaaaacggt ttaagttatt
     1321 ggaggattgc gaatgaaacg tttaattagt gcaaatccat ctgagatatt acagatgaat
     1381 gctgaagaat taaaacaaag tattttagca agtgaaggta gagttgttct atctgaaaat
     1441 gtagttactc gtgagacatt tgttggggat ataactaatt ctgaaattgc tagagctttt
     1501 ggagctgata tgattttatt gaattgtgtt gatgtttttg agcctaaaat ttatgctttg
     1561 gatagttcag gtgatgatgt tattcatcgc ttacaccagc ttgttgcttg tccaattggt
     1621 gtaaatttgg aaccgattga cccatctgca aagatgctag aggaaacaca ggaaattgtt
     1681 gcaggtcgtg ttgctagtgt tgaaacattg aagcgaatag aggagttagg ttttgacttt
     1741 gtctgtttga ctggaaatcc tggaacagga gttagcaatc gagaaatcat taaggctgtt
     1801 caaactgcta aggaaaactt ttctggtttg attattgcag gtaagatgca cggggcagga
     1861 gtgaatgagc ctgtggcaga gctttctgtc gcagagcaat tgttggaagc aggtgcggat
     1921 gtgatacttg ttccagcagt tggaaccgtt ccagcctttc atgaccaaga gttgcgtgaa
     1981 gtcgttgatc tcgttcatag taagggtggt cttgtattga gtgctattgg tactagccaa
     2041 gaaacatctg atacagatac tattaaggaa attgcactta gaaataaaat ttgtggagtt
     2101 gatattcaac atataggtga cgcaggatat gggggactgg caacagtcga taatatttat
     2161 gcattgagca aggcaattag aggagtgaga catacagtat ctcgcttggc taggtcagta
     2221 aataggtgat aaaggagtaa gctatggcta aagtaacaat tatgttagca tgtgcagcag
     2281 gtatgagtac aagtctgcta gtgacaaaga tgcaaaaggc agcagaagat aaggggttgg
     2341 atgcagaaat ttttgcagtt ccagctcctg aagcagaaga aattgtagca acaaaagaag
     2401 taaatgtgtt gcttttaggc cctcaagttc gctatttact aggggacttt caagaaaaac
     2461 taaaagatag acagattcct gtggcggtta ttccgatgac agattatgga atgatgaatg
     2521 gttctaaagt cttagattta gctgaaagtt tattagacta agattgagga gaatgctatg
     2581 aatgaaagta atttagaatc tgcaatgggg ctaattatgt atggtgggga agccaaaagt
     2641 aatgctatgg aggctattca ggcagcaaaa aaaggggatt tctctaaagc caatcgaaga
     2701 ttagctgatg cgaatgctgc cttattacag gcgcataagg ctcaaacaga aatgttgaca
     2761 aaagaggcac agggggaaga aacatcaatt agcctcttga tggtacacgc gcaagatcat
     2821 ttaatgacga gtctgacctt tgttgacttg gcgaaagaag tggtagaagt atacgaacga
     2881 tttgaaaaaa attaggaatt tgctatgaat acaatgttgg ataaaatgca agaaaaactt
     2941 tctccaattg caatgaaagt tgggaatcag aagtttttag ttgctttacg tgattcattt
     3001 gtggggacta tgcctgttat tatgacaggt tccattgctt tgttattaaa tgcttttcta
     3061 gtggatttac cacaacaatt tcacctagaa agtattacga aaacctttca gtggcttgtt
     3121 gacatcaata atttggtatt caagggaagt ataccaattg tttccttatt gttcatctat
     3181 tgcttgggag tgaatatcgc taagatttat aaggttgata cagtttctgc tggtttggta
     3241 tcgcttgctt cgtttgtcat ttcgattgga agtacagtta caaagagttt ccctttggca
     3301 aatgtaggag atgtgaaatt agatcaaatc ttacaaggaa ttgataatct agcatttgat
     3361 ggtaaaaatc tgatggtaac tattgggaat gtgattccag gaaaccatat taatgccaga
     3421 ggctacttta cagccatgat gattggattt ttagcctcta ttattttctg taaagtaatg
     3481 aaaaagaact gggtaatcaa gttaccagat tcagttcccc ctgcgattgc taagccgttc
     3541 acttctatca ttccaggatt tatggcgatg tatatagtag ccatcttgac ttatgttttc
     3601 catttactta gcaatgactt attgattgat tgggtttaca aggtgttaca aacgccatta
     3661 ctaggtttgt ctcaaagttt ctttgcagtt attctgatga tctttttgaa taagttattc
     3721 tggttttttg gtctccatgg aggaaatgta ttggctccca tcatggaagg gctgtttgga
     3781 gttgctatgt tagcgaatct ggatgctttc caaaaaggtg aaccgattcc ttatatatgg
     3841 acaagtgggt cttttggggc gtttgtctgg tttggaggat taggattagt acttgctatt
     3901 ttaatttttt caagaaacag tcattatcga aaagttgcta aacttggtct agcaccagtt
     3961 ctgtttaata ttggtgagcc ggtcaattat ggtttaccag ttgtcttgaa tcctttgcta
     4021 tttataccat ttgtacttag cccggttttc atggcaacgg tagcttactg ggcaacaagc
     4081 tggggtcttg tttcacctgt tacacaaaac gttacttggg taatgcctcc aattttatat
     4141 ggtttcttct ctacagcatt tgattggcgt gccatcatct tatcagttgt ttgtttgatt
     4201 attagtgtct tgacttattt cccatttgtg aaaatggtag ataaaactga attgagttaa
     4261 tggtcttact tgcgtttttt gtgataaaat agaaatagat tacgtgtaag atgagttagg
     4321 tgtaggattt cttatctcca ctaaaattgt tattagacaa aggtttataa tttattgaag
     4381 ttggaata
//
LOCUS       DAVLTM010000128         4375 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLTM010000128 DAVLTM010000000
VERSION     DAVLTM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.129"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            669..1661
                     /locus_tag="VYN16_001860"
     CDS             669..1661
                     /locus_tag="VYN16_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1404345.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1663..2481
                     /locus_tag="VYN16_001861"
     CDS             1663..2481
                     /locus_tag="VYN16_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1404346.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2483..3268
                     /locus_tag="VYN16_001862"
     CDS             2483..3268
                     /locus_tag="VYN16_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404347.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3366..3584
                     /locus_tag="VYN16_001863"
     CDS             3366..3584
                     /locus_tag="VYN16_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1404348.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3569..3766
                     /locus_tag="VYN16_001864"
     CDS             3569..3766
                     /locus_tag="VYN16_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404349.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4091..>4375)
                     /locus_tag="VYN16_001865"
     CDS             complement(4091..>4375)
                     /locus_tag="VYN16_001865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404350.1"
                     /translation="VVSSKHCFEVVDGTDVVSSKHCFEVVDGTDVVSSKHCFEVVDGT
                     DVVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHTHGKAALTWFEEIF"
BASE COUNT         1212 a          777 c          933 g         1453 t
ORIGIN      
        1 gatatctgat tacaaacaga atatgaaagc tttttatatc actattgaaa aatttatacg
       61 agatgatgaa agccttaagt gttattttat aaaggttatt tcaagtcgtt ccaaggtaac
      121 aagtctagat cagattgaag ctgataaaac gatacaaaga aaatattcaa gtgagctaaa
      181 aaaatttatt ggattttata atgagattat ttgtgaggaa aatagtttcc tacatgtacg
      241 aaagaggtgg tcgagttggt ttaggtagtc gatgcgtgag ttgataattc tcagggtatg
      301 gacttctttt tcatgaatga ggtaaaagag caggtattgt ttagagacaa tcattctgag
      361 catattttct ggatagaggg agtatccgat tttatgatca aagttaatac cgccctctgg
      421 tgagaagatg agtaggttgg taatttaaac tattaaacag aatttttgat taaaagtatt
      481 attttatgag agaaatccta atttcacaat ccataggcaa actcttgcat ttcgtttttt
      541 attggactat aataggttgg tataaagcct tttgtagtaa taaaatgtag aaggtgtaga
      601 aagcaaggat ttagaatatt tgtagttaaa aacacaatgt tgctattcct tacgataggg
      661 agatagatat ggcaatgata gaagtggaac atcttcagaa aaattttgtg aagactgtta
      721 aggaaccggg cttgaagggg gctttgcgct cctttattca tcctgaaaag cagacctttg
      781 aagcggtcaa ggatttgacc tttgaggttc caaaagggca gattttagga tttatcgggg
      841 caaatggtgc tgggaagtcg acaaccatta aaatgctgac aggaattttg aaaccaacat
      901 ctggtttttg tcggattaac ggcaagattc cccaggacaa tcggcaagat tatgtcaaag
      961 atattggcgt agtctttgga caacgcaccc agctatggtg ggatttggct ctgcaagaga
     1021 cctacactgt cttaaaagag atttatgatg tgccagactc gctctttcat aagcgtatgg
     1081 actttttgaa tgaagtcttg gatttgaagg actttatcaa ggatcccgtg cggactcttt
     1141 cactgggaca acggatgcgg gcggatattg cggcctcctt gctccacaat cccaaggttc
     1201 tttttttaga tgagccgacc attggtttgg acgtttcggt taaggataat attcgtcggg
     1261 caattactca gatcaatcaa gaggaagaaa ctaccattct tttgaccact cacgatttga
     1321 gtgatattga gcaactttgt gatcggattt tcatgattga caaggggcaa gagatttttg
     1381 atggaacggt gagccaactc aaggagacct ttggtaagat gaagactctc tcttttgaac
     1441 tgctaccagg tcaaagtcat ctcgtctctc actatgaagg tctgtctgat atgaccattg
     1501 atagacaagg aaacagcctc aacattgaat ttgatagttc tcgctaccag tcagctgaca
     1561 ttatcaagca aaccctgtct gattttgaaa tccgcgattt gaagatggtg gatacggata
     1621 ttgaggatat tatccgtcgc ttctaccgaa aggagctcta ggatgatcaa attgtggaga
     1681 cgttataaac cctttatcaa tgcaggggtt caggagttga ttacttaccg agtcaacttt
     1741 attctctatc ggattggcga tgtcatgggg gcttttgtgg ccttttatct ctggaaggct
     1801 gtctttgatt cttcgcaaga gtctttgatt cagggcttca gtatggcgga tatcaccctc
     1861 tacatcatca tgagttttgt gaccaatctt ctgactagat ccgattcgtc ctttatgatt
     1921 ggggaggagg tcaaggatgg ctccattatc atgcgtttgt tgcgaccagt gcattttgcg
     1981 gcctcctatc ttttcaccga gcttggttcc aagtggttga tttttatcag cgttggcctt
     2041 ccatttttaa gtgtcattgt cttgatgaaa atcatatcgg gtcaaggtat tgtagaggtg
     2101 ctaggattaa ctgtccttta tctttttagc ttaacgctcg cctatctgat taactttttc
     2161 tttaatattt gctttggatt ttcagccttt gtgtttaaaa atctttgggg ttccaaccta
     2221 cttaagactt ccatagtggc ttttatgtcg ggaagtttga ttcccttggc attctttcca
     2281 aaggttgttt cagatattct ttcctttttg cctttttcat ccttgattta tactccagtt
     2341 atgatcattg ttggaaaata cgatgccagt cagattcttc aggcactcct tttgcagttc
     2401 ttctggctct tagtgatggt gggattgtct cagttgattt ggaaacgggt ccagtccttt
     2461 atcaccattc aaggaggtta gtatgaaaaa atatcaacga atgcatctga tttttatcag
     2521 acaatacatc aaacaaatca tggaatataa ggtggatttt gtggttggtg tcttgggagt
     2581 ctttctggct caaggcttga atctcttgtt tctcaatgtc atctttcaac atattccatt
     2641 cctagaaggt tggacctttc aagagatagc tttcatttat ggattttcct tgattcccaa
     2701 gggaatggac catctctttt ttgacaatct ctgggcacta gggcaacgcc tagtccgaaa
     2761 aggagagttt gacaagtatc tgactcgtcc catcaatcct ctctttcaca tcctagttga
     2821 aacctttcag attgatgcct tgggtgaact cttagtcggt ggtattttat tgggaacaac
     2881 agtgaccagc attgtttgga ctcttccaaa atttctgctt ttcctagttt gtattccttt
     2941 tgcgaccttg atttatactt ctcttaaaat cgcaacagcc agtatcgcct tttggactaa
     3001 gcagtcaggc gccatgattt acatcttcta tatgttcaat gactttgcta agtatccgat
     3061 ttctatttac aattctcttc ttcgttggtt gattagcttt atcgtgcctt tcgcctttac
     3121 agcctactat ccagctagct atttcttaca ggaaaaggat gtgttcttta acgtaggagg
     3181 tttgatgttg atttctctgg ttttctttgt tatttccctt aaactttggg ataagggctt
     3241 agattcctac gaaagtgcgg gttcgtaaaa gctaaagtaa gactaaaatc aagaaaggaa
     3301 cttatgatgt ttgtaattga agaagtcaag gatgaaaatc aaaaaaggca gttgtcgctg
     3361 agattttgaa ggatttgcca gaatggtttg gaatcccaga aagcacacaa acctatatag
     3421 aaggaaccac gacactgcaa gtttggaccg cctatcagga gagtgatttg actggatttg
     3481 taagcttatc ctattcgagt gaagattgtg cagagattga ttgtctcggc gtaaaaaagc
     3541 ttatcaaggt agaaaaattg ggagccaatt gcttgctact ttagagagtg aagctcgtaa
     3601 aaaagttggt tatctgcagg tcaaaacagt ggcagaagct tctaataaag attatgatcg
     3661 aacaaatgac ttttatcgag gtcttggctt taaaaagtta gagatttttc ctcaactatg
     3721 gaatccgcaa aatccttgtc agattttgat taaaaagctt gaataatatt acttgacatc
     3781 tattttttat tctcagagtg ctatactgta agtgtaatcg ccgatttagc tcagttggta
     3841 gagcaaggca ctcgtaaagc ctaggttata ggtagataaa cgactgagga gttgaaaaaa
     3901 tagataggta gaagataacc gttaagcctt attcttagcg gttatttata ttgtttaata
     3961 gcgctgatat ttcatcaatt atgcctgttt tcgtgtttcc ggtagttgtt caagtttatt
     4021 gctactattt ttgatggtat gaatgtgctt ataatgtatc ccggttaacg aaagttttgg
     4081 acttatactc ttagaaaatc tcttcaaacc acgtcaacgc cgccttgccg tgcgtatggt
     4141 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg acttcgtcag
     4201 ttctatccac aacctcaaaa cagtgttttg agctgactac gtcagttcca tctacaacct
     4261 caaaacagtg ttttgagctg actacgtcag ttccatctac aacctcaaaa cagtgttttg
     4321 agctgactac gtcagttcca tccacaacct caaaacagtg ttttgagctg actac
//
LOCUS       DAVLTM010000129         4305 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLTM010000129 DAVLTM010000000
VERSION     DAVLTM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.130"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            33..1022
                     /gene="manX"
                     /locus_tag="VYN16_001866"
     CDS             33..1022
                     /gene="manX"
                     /locus_tag="VYN16_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1404351.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1050..1853
                     /locus_tag="VYN16_001867"
     CDS             1050..1853
                     /locus_tag="VYN16_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1404352.1"
                     /translation="MSIISMVLVIVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1877..2788
                     /locus_tag="VYN16_001868"
     CDS             1877..2788
                     /locus_tag="VYN16_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1404353.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2844..4178)
                     /gene="pepC"
                     /locus_tag="VYN16_001869"
     CDS             complement(2844..4178)
                     /gene="pepC"
                     /locus_tag="VYN16_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1404354.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
BASE COUNT         1175 a          928 c          907 g         1295 t
ORIGIN      
        1 aaaactaaaa caaaaaagga ggaatgacaa taatgagtat cggaatcatt attgcgagcc
       61 acggcgaatt tgctgctggt attcatcagt caggatctat gatctttggt gaacaagaaa
      121 aggttcaagt tgtgaccttt atgccaaatg aaggtcctga tgatctatac gctaagttta
      181 ataacgctgt tgctgcattt gacgcagaag atgaggttct agttttggct gacctttgga
      241 gtggttctcc atttaaccaa gctagtcgcg tgatgggaga aaatcctgag cgtaagtttg
      301 ccatcatcac aggacttaac ttaccgatgt tgattcaagc ctacacagag cgcctcatgg
      361 acgctgctgc aggtgtagaa aaagtcgctg ctaatatcat taaagaagcc aaagatggca
      421 tcaaagctct tccagaagag ctaaatccag ttgaagaagt tgcaagcgct gcagctgctc
      481 cagttgccca aactgctatc ccagaaggaa ctgttatcgg agacggtaaa ttgaaaatca
      541 atcttgcccg tcttgacaca cgtctacttc acggtcaggt tgcaactgct tggactccag
      601 attcaaaagc aaatcgtatc atcgttgctt cagataacgt ggctaaagac gaccttcgta
      661 aagaattgat taaacaagca gctccaggta atgtcaaggc taacgtggtt ccaattcaaa
      721 aactgattga gatttcaaaa gacccacgtt ttggagaaac acatgccctt atcttgtttg
      781 aaacacctca agatgccctt cgtgccatcg aaggcggcgt gccaatcaag actcttaacg
      841 ttggttctat ggctcactca acaggtaaaa cattggtcaa taccgttttg tctatggaca
      901 aagaagatgt tgctacattt gaaaaaatgc gtgacttggg tgttgaattt gatgtccgta
      961 aagtaccaaa tgattctaaa aaagatttgt ttgacttgat taacaaagcc aatgtcaaat
     1021 aagccattat ttatgaaagg attttaaaca tgtctattat ttctatggtt ttagtaatcg
     1081 ttgtagcctt ctttgcaggt cttgaaggca tcctcgacca gttccaattt caccaaccac
     1141 ttgtagcctg tacccttatt gggcttgtaa caggtcactt ggaagcaggg attatcctcg
     1201 gtggatcgct tcaaatgatt gcccttggtt ggtcaaatat cggtgctgct atcgctcctg
     1261 atgctgcact tgcttctgtc gctgctgcca ttatcatggt tcttggtggt gactttacca
     1321 agactggtat cggtgttgcc caagcggttg ctatccctct tgccgtagct ggacttttct
     1381 tgacaatgat tgttcgtaca atttcagttg gtttggttca tactgcagat gctgccgcta
     1441 aaaaaggtga cttcggcgct gtggagcgtg cgcatttcat tgcgctactt ttccaaggac
     1501 ttcgtatcgc gcttcctgca gctcttctcc ttatggtacc aactgaaact gtacaaagta
     1561 tccttagtgc catgccagac tggctcaaag atggtatggc tatcggtggt ggtatggtcg
     1621 ttgccgttgg ttacgccatg gttatcaaca tgatggcaac tcgtgaagta tggccattct
     1681 tcgctcttgg tttcgttctc gctgctgtgt cagatattac tctaatcgga ttcggtgcta
     1741 tcggtgttgc tatcgctctt atctaccttc acctttctaa aactggtgga aatggtggcg
     1801 gaggagccgc aacttctaac gacccaatcg gcgatatcct agaagactac taagataaga
     1861 aaggactgaa aacatcatga ctgaaaaact tcaattaact aaatcagatc gtaaaaaagt
     1921 ttggtggcgt tcaaccttct tacaagggtc ttggaacttt gaacggatgc aaaacttggg
     1981 ctgggcttat acactcattc cagctatcaa aaaactctat actaaaaaag aagatcaaat
     2041 cgctgctctt gagcgtcacc ttgagttctt caacactcat ccatacgtag ctgctccagt
     2101 catgggggtt actcttgcgc ttgaagaaga acgtgctaac ggtgtggaaa tcgatgacgc
     2161 tgctatccaa ggggttaaaa tcggtatgat gggacctctt gctggtatcg gtgacccagt
     2221 attctggttt acagtacgcc caatccttgg atctctcggt gcttcacttg cccttactgg
     2281 caatatcttg gggccactcc tcttctttgt tgcatggaac ttgattcgta tgtcattctt
     2341 gtggtatgtt caagagattg gatacaaggc tggatcagaa atcactaaag atatgtctgg
     2401 tggtatcctt caagatatca ctaaaggagc ttctatcctt gggatgttca ttcttgctgt
     2461 ccttgttcaa cgctgggtaa atattaaatt tgctttcgat gtttctaaag ttcaactaga
     2521 tgaaaaggct tatatccatt gggataaatt gccagaaggg tctaaaggta tccaagaagc
     2581 attcgcacaa gtaggacaag gattgtctca aactcctgaa aaagttacta ctttccaaca
     2641 aaacttggat atgttgattc ctggattatc aggactactc cttactttac tttgcatgta
     2701 cttacttaag aaaaaagtat ctccaatcac tattatcctt gccctcttcg cagtgggtat
     2761 tgtggcacat gttcttcaca tcatgtaatc aagcaactaa aaaggaacca ggttctaaaa
     2821 tctgattcct tttttctatg cttttattta gccaaggctc ccattggatc ccatggtgca
     2881 agtacaattg gttctgctcc ataggcagct tgttcttctg ctgtcagcaa ttccttacga
     2941 acaacgattt ggtatgtgta ttcgtccatc caagcgtctg aggcaacaaa gtaaccatct
     3001 gtaccgacct tgtctcccca tgagttttca accttccact tggttgattt accattttcg
     3061 tccaagtcaa cacctgtcaa gaccatggcg tgggtcatca agctttcgct atagtccaaa
     3121 cgtccagcct tgtcttgagt aagtttaatg tccatgcttg attcaaagtc ataaacatct
     3181 gtcgcaagga ttccagcctt acggttactg agctggccga catcagaacc aaaccaaaca
     3241 gtctcacctg cttgcatttg ggcaatcgcc aattctttca agcgctccat tggaacgttg
     3301 atgtaacgaa ctgcacggct accaaccaca ttccccaaca tctcaactgt gtaagatttt
     3361 ccgtaaggtt tatcagcagt tggagcattg ataacagaaa cgtagtcttc taaaggaaga
     3421 ttgacatatt tcttgtagaa ctcttgtggt gtgattcctt tttcactttt gtagttgtta
     3481 tctttatcgc gataagcaaa gtcaaacttg cgtggtggaa gtcctaatga catagcaaga
     3541 aagttaaaga tttcttgcaa gaggtcttct ttcttagctt gaacagtcac ttgatctgca
     3601 ccagaaacaa gcaaatcacg caagatttga gcatcttgac gaagcaattt attaaggatc
     3661 gcatttagct cacgactgct gctagatgaa acagactcag gataaactga cttaggcacg
     3721 acaccgtatt tttcaaagag ggaaacgacc atatcccatt gaccaccatc ttgttgaggt
     3781 gtttggagta agaagctaac cttgcggcta gtcaattctt ggtctgaagt cgcaatgact
     3841 tgctccaaga accagtttga tttctcatac ttatcccaga agaaagtgtg ggcttgtgac
     3901 aactcaaagt tctccaattt gtattgcgag atgagtttgt ggcggaaggt gttgagagcc
     3961 gcaaacatcc agcaacgacc agacgctttc tggttagtga ccttgtcctt ggttaaatcc
     4021 aatgagaaaa caggtgtgtt gtctacatgg ctttggcgac gttccagagc tgcaaaaatt
     4081 ccgttgtggc tggcagcatt ttcaatcgct tggtatttta catttgcttc atagttggca
     4141 aatagtttat cagtaaatga ttcttgaatc gcgttcatag attcctcctt ttattctaca
     4201 gtctattata actcttttca caaaggaagc aactaaaaaa cataaaaggt aaggacattc
     4261 tccctcacct ttttcgaatt tatactcttc gaaaatctct tcaaa
//
LOCUS       DAVLTM010000130         4298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLTM010000130 DAVLTM010000000
VERSION     DAVLTM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.131"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(66..1154)
                     /locus_tag="VYN16_001870"
     CDS             complement(66..1154)
                     /locus_tag="VYN16_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1404355.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1172..1840)
                     /locus_tag="VYN16_001871"
     CDS             complement(1172..1840)
                     /locus_tag="VYN16_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1404356.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1859..4297)
                     /locus_tag="VYN16_001872"
     CDS             complement(1859..4297)
                     /locus_tag="VYN16_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1404357.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQYILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
BASE COUNT         1350 a          899 c          659 g         1390 t
ORIGIN      
        1 ttctatagaa ttcaatacag ggatcaataa aagactacca tttggaaaac agtagtcctt
       61 attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc aacatctgaa
      121 ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc ttgtttacca
      181 acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag agttgttggc
      241 ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg tctattttcc
      301 aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa atgatgaatg
      361 tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa tcctccactt
      421 tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa tgctggtgtc
      481 accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag cgtttcttca
      541 catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat agtttttcca
      601 tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc aataccagac
      661 gctaataaac gcttaggggc ttgtgaaata acttttgtat caaccaaaac taaatctgga
      721 tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat aacagataaa
      781 gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg cttttcaatc
      841 aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc gataatacta
      901 tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc agaagcttca
      961 ccattaaata gcgccagaac aatatggaaa ccatacctat gtaggtaatc ttcaaatcgt
     1021 tttccaacaa tatcataaac caactgatcg cataatagaa tagggcaatt tcccaaatcc
     1081 aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct agaaggacta
     1141 gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt tgaataacaa
     1201 accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg aaagctgatt
     1261 caaaaacatc cgctcctgct gtaacagcat gcgcacctgc agctaaagca ttatttactt
     1321 gtgctacatt tttaaaggat gcagccaaaa tcttactagg agagttctgt ctatcaatag
     1381 caagaactaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc attctattat
     1441 aatatggagc taggtaatcc gctcctgctt cgatagctaa taatccctga ataactgtat
     1501 aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt gcacgtaatc
     1561 cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt cgaatttcat
     1621 gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga atagagggtg
     1681 tagagccaat caattctctt acatctttga ttcgttcaaa aaaattaata gaaccctctc
     1741 tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca gaccactttt
     1801 taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc tctttatttt
     1861 acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa agaacattga
     1921 agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca ggatgtttct
     1981 gagcgtcaat caacgtctct ctggaaacaa cattgtattg aatatggtac ccatgtaaac
     2041 gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct ttggctaacg
     2101 tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct gttggtaatt
     2161 ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt gatggtgaac
     2221 aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat gttccacgtc
     2281 cctgccctac gttggctgag atagaagatg ttcctgaata acgaattcct ccaattggac
     2341 ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg tcataagcag
     2401 cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca tcatgaatca
     2461 acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccagt gcatgccaaa
     2521 gctgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca gctaatgaat
     2581 ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact gctccacctt
     2641 ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat agaatatcag
     2701 gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc attcgtgtca
     2761 aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc atatccttaa
     2821 aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca tcattcatcg
     2881 tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga tagccccatt
     2941 tccctggaac tgccgtttca acacatccaa tggcactgta atcataagca tcatcttcca
     3001 atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat gcaggcatac
     3061 caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct aaacctgcat
     3121 ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat tttaatacca
     3181 aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca attgtaacat
     3241 tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga accttattaa
     3301 ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct tctgtttctt
     3361 taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa cggccatatg
     3421 aaagagagtg gccattagat tcaatttgta aaatatattg aataaaccaa actgattgaa
     3481 tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata tctgcaatct
     3541 caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta gcaagagcaa
     3601 caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag tcgtaaaaat
     3661 gatatttatc aatacttgct ggatctgtta aatctagagc tacttttgct ttacgagccc
     3721 gctcttcaaa acctcttaaa ccaaattgca aaagtttctg atagttaact gctaagtgag
     3781 catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata taaactgaca
     3841 cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc caaaacggag
     3901 caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct ccatcacgct
     3961 tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga aaaataggag
     4021 catctttatt ggaagaagct tgatttcccg caatcataga ttcttcttca atatagatag
     4081 acatattttc caaaatttct ttcagcatat atgcacgttt taggacatta ggttgttcct
     4141 tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata taaggttttt
     4201 tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca aaatattctg
     4261 ttttgattgt tgttcttgct acttctgtat ttaccatc
//
LOCUS       DAVLTM010000131         4248 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLTM010000131 DAVLTM010000000
VERSION     DAVLTM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.132"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            453..1496
                     /locus_tag="VYN16_001873"
     CDS             453..1496
                     /locus_tag="VYN16_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1404358.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1663..3750
                     /gene="topA"
                     /locus_tag="VYN16_001874"
     CDS             1663..3750
                     /gene="topA"
                     /locus_tag="VYN16_001874"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1404359.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEVASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3856..4215
                     /locus_tag="VYN16_001875"
     CDS             3856..4215
                     /locus_tag="VYN16_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1404360.1"
                     /translation="MRLIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
BASE COUNT         1315 a          731 c          905 g         1297 t
ORIGIN      
        1 ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt atgttcaatg
       61 attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa gtagagatta
      121 caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa tggttattat
      181 aagaaatggt ctaaaataga tgatgatact tttcgaaaat ctttttacgt cagctcagct
      241 ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt tcatttacta
      301 gaaatgaaac tgatgagaga tatcagtaga catttgagtc aggatattat ggaaaatgat
      361 aaaaagagct tgtgagattg gcatatcaga ctactaaagt attgagtttg ttaggatttt
      421 agcgactagt tagctgggaa aggaagatat ttgtgacaaa taataaactg tattcgttga
      481 tagaatttag aaataaaata tatgaagaat tagaactttc cagaagtgat ttagcgattt
      541 tactatgtgc catgcttatc gcctctatcg gattaaatat ggattcgact cccgtgatta
      601 ttggagccat gttaatctct cctttgatga cacctattct gggagtgggg ctctctctag
      661 ctatatttga ttttaaattg ttaagaaaat cttttaaaat attagctatt caaattcttg
      721 ccagtctaat agcttcaaca ctttattttt atctttctcc catttcgtat gctagttcgg
      781 agattgttgc tagaacctct ccgactattt gggatgttct cattgctttt gtaggaggga
      841 tagcaggtat tattggtgct aggaaaaaag agaccaataa tattgttcct ggtgttgcta
      901 ttgcaaccgc cttgatgcct cctctttgta cagtaggtta tgctattgct tctgctaatc
      961 taaaatttat cataggctcc tcttacctat tcctcatcaa ttgtagcttt attgtcattg
     1021 cgacttatat aggtgttagg ttgatgatgg ttaagaaaca ttattttaaa gataatgaag
     1081 aagactctaa aatgcgtagg attttgcttc tagttgctgt tttgctgatg attccgagtt
     1141 tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa agagtccctt aagaaattta
     1201 tatcagagca gtttcagggg tataatattt tgaaaaaaac ctattctaaa aagactcata
     1261 ccctaaagct aaccatttca ggaaattatt tgacagaaga agaactcgat atgatttcaa
     1321 gtaagagagg tgactatggt ttaagtgatg tttctgttca agtttcacaa ttgtctgatt
     1381 cagaacaact tagtaaggaa gagctggtgg agtatttctt ccagtatatc aaggataagg
     1441 aagcaaaaga aaaggaaaaa gctaataagt tttatacaga gtctgaggag caataatttc
     1501 ttgagaatag ctggtttttc tcgtgagtct tctatgtata tcaaaggaag actgaggttt
     1561 taagtataaa acttttcttc tattatagta gctaagtctt gacagggttc aaaaaatggt
     1621 ttacacttta taaagtttat tactttgaaa aggtgtgata ctgtggctac ggcaacaaaa
     1681 aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg agtcgcctgc taaggccaag
     1741 acgattgaaa aatatctagg cagaaactac aaggttttag ccagtgtcgg gcatatccgt
     1801 gatttgaaga aatccagtat gtccgtcgat attgaaaata attatgaacc gcaatatatt
     1861 aatatccgag gaaaaggccc tcttatcaat gacttgaaaa aagaagctaa aaaagctaat
     1921 aaagtttttc tcgcgagtga cccggaccgt gaaggagaag cgatttcttg gcatttggcc
     1981 catattctca acttggatga aaatgatgcc aaccgtgtgg tcttcaatga aatcaccaag
     2041 gatgcagtca aaaatgcttt taaagaacct cgtaagatcg atatggactt ggtcgatgcc
     2101 caacaagctc gtcggatctt ggaccgcttg gtagggtatt cgatttcgcc tattttgtgg
     2161 aagaaggtca agaagggctt gtcagcaggt cgcgttcagt ccattgccct taaactcatc
     2221 attgaccgtg aaaatgaaat caatgccttc cagccagaag aatactggac agttgatgct
     2281 gtctttaaaa agggaaccaa acaatttcat gcttccttct atggagtaga tggtaaaaag
     2341 atgaaactga ccagcaatga cgaagtcaag gaagtcttgt ctcgtctgac gagtaaagac
     2401 ttttcagtag atcaggtgga taagaaagag cgcaagcgca atgctccttt accctatacc
     2461 acttcatcta tgcagatgga tgctgccaat aaaatcaatt tccgtactcg aaaaaccatg
     2521 atggttgccc aacagctcta tgaaggaatt aatatcggtt ctggtgttca aggtttgatt
     2581 acctatatgc gtaccgattc gactcgtatc agtcctgtag cgcaaaatga ggtggcaagc
     2641 ttcattacgg atcgttttgg tagtaagtat tctaagcacg gtagcaaggt caaaaacgca
     2701 tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa gtgtctttaa tacaccagaa
     2761 agcatcgcta agtatctgga caaggatcag ctcaagctat atacccttat ctggaatcgt
     2821 tttgtggcta gccagatgac agcggccgtt tttgatacca tggctgttaa attgtctcaa
     2881 aaaggggttc aatttgctgc caatggtagt caggttaagt ttgatggtta tcttgccatt
     2941 tataatgatt ctgacaagaa taagatgtta ccggacatgg ttgttggaga tgtggtcaaa
     3001 caggtcaata gcaaaccaga gcaacatttc acccaaccgc ctgcccgtta ttctgaagca
     3061 acactgatta aaaccttaga ggaaaatggg gttggacgtc catcaaccta cgcgccaacc
     3121 attgaaacca ttcagaaacg ttattatgtt cgcctggcag ccaaacgttt tgaaccgaca
     3181 gagttgggag aaattgtcaa taagctcatc gttgaatatt tcccagatat cgtaaacgtg
     3241 accttcacag ctgaaatgga aggtaaactg gatgatgtcg aagttggaaa agagcagtgg
     3301 cgacgggtca ttgatgcctt ttacaaacca ttctctaaag aagttgccaa ggctgaagaa
     3361 gaaatggaaa aaatccagat taaggatgaa ccagctggat ttgactgtga agtgtgtggc
     3421 agtccaatgg tcattaaact tggtcgtttt ggtaaattct acgcttgtag caatttccca
     3481 gattgccgtc atacccaagc aatcgtgaaa gagattggtg ttgagtgtcc aagctgtcat
     3541 cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc tcttctatgg ttgcaatcgc
     3601 tatccagaat gtgaatttac ctcttgggac aagcctgttg gtcgtgactg tccaaaatgt
     3661 ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca agcaggttgt ttgtagcaaa
     3721 ggcgactacg aggaagaaaa gattaaataa gaggaggggc ccgaaagtaa atttcaggct
     3781 ccttttggtt agaacttgac aaaatacatc attttatgcg aaactagaag aagattattt
     3841 taactaggag aagttatgcg tcttatctat ctgataattg gttttttatc actgaccttg
     3901 gctattgttg gggttgtttt acccttgttg ccgacaacgc ctttcctttt gttgtctatt
     3961 gcttgtttct ccagaagttc caagcgtttc gaagactggc tttatcatac caagctctat
     4021 caaacatatg tagctgattt tcgcgagact aagtctatta cgcgtgaacg aaagaaaaaa
     4081 atcatcgtat ccatttacat tttgatgggg atttctattt attttgcccc tctcttacca
     4141 gtcaaaatcg gtctgggtgc tttgaccatc tttatcactt attatctttt caaggtcatt
     4201 ccagacaaag aatagttaaa acagtagtta tttgccttga taaaattg
//
LOCUS       DAVLTM010000132         4020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLTM010000132 DAVLTM010000000
VERSION     DAVLTM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.133"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            17..826
                     /locus_tag="VYN16_001876"
     CDS             17..826
                     /locus_tag="VYN16_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1404361.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            831..1532
                     /locus_tag="VYN16_001877"
     CDS             831..1532
                     /locus_tag="VYN16_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1404362.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1557..2474
                     /locus_tag="VYN16_001878"
     CDS             1557..2474
                     /locus_tag="VYN16_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV1404363.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2502..>4020
                     /locus_tag="VYN16_001879"
     CDS             2502..>4020
                     /locus_tag="VYN16_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1404364.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKAD"
BASE COUNT         1300 a          623 c          783 g         1314 t
ORIGIN      
        1 caactaagga gctcatatgt tactgcaaaa agaactaatt ccaatgatag aagctaactt
       61 accaaatatg gcatatgctg aaaaagacat tgctaaattc ttcttaaaac agcaacctct
      121 gaatgattat tcatctaaag cattgtgcga ataccttaat gtatccaaag caacattgac
      181 tcgatttgcg aaaaaatgtg gttttaaagg ttttagacaa ttcattttca aataccaaga
      241 gatgattcat gagaaagaaa agttggcatt atatacagag gcaacagaaa aagttttatc
      301 cgactatgag gaaatgttga gaaaaactta cacggttctt gatgaagttc aacttgagcg
      361 tattgctgag atgatagaaa ctgctgagcg tgtatatctc tacggtaaag gaagttctgt
      421 tcttgcttta caagaaatga agatgagatt tatgcgtctc ggagtgattg gtgaagtatt
      481 atcagacgag gatatgattt tgtggagtag cttactactt aatgaaaatt gccttgtcat
      541 tggagcatcc atttcaggtc aaactgatat tgtactagaa ggtctacaaa aagctgcaga
      601 taaaggcgct aaaacagttt taatgactac aagaaaattt gacgaagaag attgtttctt
      661 tgatgaacta ttgttattag cttcgaccga tcatctctcg tatggcaatc gcatatcacc
      721 tcagtttcca atacttttaa ttacagactg cttattctct aattatctgg aaagtccaga
      781 gagacaatat tattacaatc aaactattat ccataaggag gaataataaa atgagccaaa
      841 tttggactaa agaaaaattt ataagccaag ttcaaggtgg agtcattgtt tcttgtcaag
      901 ctttacctgg tgaagccctt tataatgaag aatttagctt gatgcctttt atggctaaag
      961 cagctttaga ggcaggagca gtgggcattc gcgcaaattc tgtgcgtgat attaaagcaa
     1021 ttcagaaagt agtagattta ccaataattg gaattatcaa aagggattat ccacctcaag
     1081 aaccatatat tactgctacg atgaaagaag tagatgaact tgtagaatgc ggaacaacag
     1141 tcattgcatt tgatgcaact ttaagaccaa gatatgatgg cttagttgtc agtgaattta
     1201 tcaaaaaaat aaaagaaaaa tatccgaatc aattgctgat ggcggatgta agtaatttag
     1261 atgaaggtct ctatgcattt aaatcaggcg ttgattttgt tggtacaaca ttatcaggtt
     1321 acacaagtac aagtgtacaa tcagatgagc ctgattttga actaatgaaa aaattggctg
     1381 attttaatat tccggtaatt gccgaaggaa aaattcatta tccagaacaa ttaaaaaaag
     1441 cttatagttt aggtgttacc agtgtagtca ttggtggagc gattacacgt ccaaaagaaa
     1501 ttgctcagcg atttattaat gtcatcaaat aaaaaacaat ataaaggaga aatctgatga
     1561 aagatttaac taaatacaaa ggcgttatcc ctgcatttta tgcttgctat gatgaaaatg
     1621 gtgaaattag ccaagatcgt gtaaaatctc tggtacaata tttcattgac aaaggtgtaa
     1681 aaggtatcta tgtaaatggt tcttcaggtg aatgtattta ccaaagtgta gaagatcgta
     1741 aacaaattat tgaagctgtt atggaagttg ctaaaggtaa attaacagtt atcaaccata
     1801 ttgcatgtaa taacacgaaa gatagtatcg aattggcaaa acattcagaa agtgttggag
     1861 tcgatgctat tgcagctatc ccacctattt atttcaaatt gccagagtat tcaatcgcag
     1921 catattggaa tgcaatgagt gaagctgcgt caaatacaga ttttattatc tataatattc
     1981 cacaattggc aggggttgcg ttgactggta gtttgtatgc aacaatgcgt caaaatcctc
     2041 gtgtgattgg agttaaaaat tcttctatgc ctgtacaaga tattcaaatg tttgtagctg
     2101 caggtggaga agattacatt gtattcaatg gtccagatga acaatatctt ggtggtcgct
     2161 tgatgggagc agaagctggt attggtggta cttatggcgt tatgccagat ttgttcttga
     2221 aattggaaag tttgattcaa gaacgagatt tagatacagc taaaaaactt caatatgcta
     2281 tcaatgaagt tatctataag atgatatcag gtaaggcaaa tatgtatgct gtagcaaaag
     2341 aagttttgcg tctaaatgaa aaacttgatt taggttctgt tcgtcaacct ttagaagcat
     2401 tggcagaagg tgacttggaa gttgcaaaac aagcagcaga acttattcaa caagcacgaa
     2461 aagaattttt ataataaata aaatcaatat ggagatatgt tatgggcaca acaggattta
     2521 caataattga cttaattatc ttgattgttt atttacttgc ggtgttggtt gcaggtatct
     2581 atttctctaa aaaagagatg aaaggaaaag agttctttaa aggagatggt tcggttcctt
     2641 ggtatgttac ttcggtatcc atttttgcca caatgctcag tccgatttcc ttcttgggac
     2701 tcgctggtag ctcttatgca ggtagctgga ttttatggtt tgctcaatta gggatggtag
     2761 tagctattcc actgacaatt cgttttatct tacctatctt tgcacggata gacatcgata
     2821 cggcatatga ttacttggat aaacgtttta attctaaagc acttcgtatt atttcagcac
     2881 tcttgtttat tatttatcaa ttgggacgta tgtctatcat tatgtacctc ccatcagctg
     2941 gtttatcagt attgacagga attgacatca atattttgat tattttgatg ggtgtagttg
     3001 caattgttta ttcttatact ggtggtctaa aatccgtatt atggacagac tttattcaag
     3061 gtgtgattct gattagtggt gtcgttttag ctttatttgt actgattgct aatattaaag
     3121 gtggctttgg tgcagtagca gaaacattag caaacgggaa attccttgct gcaaatgaaa
     3181 aacttttcga tcctaacttg ctttcaaact ccatcttttt aattgtgatg ggttcaggct
     3241 ttacaatctt gtcttcctat gcttcatctc aagatttggt tcaacgtttt actacaacac
     3301 aaaatattaa gaaacttaat aagatgttgt tcacaaacgg tgttttgtca cttgcaactg
     3361 caacagtctt ttacttgatt ggtacaggct tgtacgtatt ctatcaagta caaaatgcag
     3421 atagtgcagc tagcaatatc cctcaagacc aaatctttat gtactttatt gcataccagt
     3481 taccagtagg tatcacaggt ttgatcttgg cagcgattta tgcagcatct caatcaacta
     3541 tttcaacagg tttgaactct gttgcaactt catggacatt ggatattcaa gatgtcattt
     3601 ctaaaaatat gtcagacaat cgtcgtacga aaattgcaca attcgtatct ctagcagtag
     3661 gtttattctc aattggtgtt tccattgtca tggctcactc agatattaaa tctgcatacg
     3721 aatggttcaa tagtttcatg ggacttgtac ttggtctact tggtggtgta tttattcttg
     3781 gatttgtttc taaaaaagca aataaacaag gtgcttatgc agcgctgatt gtatcaacca
     3841 tcgtcatggt atttattaaa tacttccttc ctccaacagc tgttagctac tgggcatatt
     3901 cattgatttc aatctctgta tcagtagttt caggttatat tgtatctgtt cttactggaa
     3961 ataaagtatc tgcacctaaa tatacaacga ttcatgatat tacagaaatt aaagcggatt
//
LOCUS       DAVLTM010000133         3984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLTM010000133 DAVLTM010000000
VERSION     DAVLTM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.134"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            37..1812
                     /locus_tag="VYN16_001880"
     CDS             37..1812
                     /locus_tag="VYN16_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV1404365.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQALGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDDKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSENERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIIEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIQLILETGGL"
     gene            1813..3198
                     /locus_tag="VYN16_001881"
     CDS             1813..3198
                     /locus_tag="VYN16_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV1404366.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVKFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3221..3832
                     /locus_tag="VYN16_001882"
     CDS             3221..3832
                     /locus_tag="VYN16_001882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV1404367.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKAFAVAKSLKNSLMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1291 a          588 c          869 g         1236 t
ORIGIN      
        1 attctataat ttttggactt agtaaggaga ataactttga ctcaagggaa gattataaaa
       61 gtatcgggac ctctagttat tgcatcaggt atgcaggagg ctaatattca agatatttgc
      121 cgtgtaggta agctagggtt aatcggtgaa attattgaaa tgagaagaga tcaggcatct
      181 atccaagtct atgaagaaac atctggtctt ggtccgggag aacctgttgt tacaactgga
      241 gaacctctct cggttgaatt agggcctgga ttgatttctc aaatgtttga tggcatacaa
      301 cgcccattag atcgatttaa attggctact cataatgatt ttctagttcg tggggtagaa
      361 gttccaagtt tggatagaga tattaagtgg cattttgatt ccactatatc aattggtcaa
      421 aaagtgagta cgggtgatat tcttggaact gtcaaggaaa ccgaagtagt taatcataaa
      481 attatggttc cttatggaat atctggcgaa gtcgtttcta ttgcctctgg cgattttaca
      541 attgatgaag ttgtatatga aataaaaaaa ttggacggta gtttctataa aggaacactt
      601 atgcaaaaat ggcctgtccg caaggcgcgt cctgtttcta aacgtttaat cccagaagaa
      661 ccattaatca caggtcaacg agttattgat acattctttc cagtaaccaa agggggagca
      721 gcagctgttc ctggaccgtt tggagcagga aagacagttg tacaacacca agtagctaaa
      781 tttgccaatg ttgatattgt tatttatgtc ggttgtggag aacgtggaaa tgaaatgacg
      841 gatgtactga atgagtttcc tgagttgatt gaccctaata caggacaatc aattatgcaa
      901 cggacagttc tgattgctaa tacttcaaat atgcctgttg ctgctcgtga ggcttcaatt
      961 tatacaggaa ttaccatggc tgagtatttt cgtgatatgg gctactctgt cgccattatg
     1021 gctgattcga cttcacgttg ggcagaagcg ctacgtgaaa tgtcaggacg tctagaagaa
     1081 atgcctggtg atgagggtta tcctgcttat cttggaagtc gtatcgctga atattatgaa
     1141 agagcgggac gttctcaggc tctagggctt ccagaacgtg aaggaacgat tactgctatt
     1201 ggagctgtat cgccacccgg tggagatatt tcagaaccag ttactcaaaa cactttacgg
     1261 attgtgaaag ttttttgggg gcttgatgct ccgttggcac agcgacgtca ttttcctgca
     1321 attaactggc ttacctctta ttcactctat aaagacagtg tgggaactta tatagatgat
     1381 aaagagaaga cagattggaa tagtaaaata actcgtgcga tgaactactt acaacgggaa
     1441 tctagtttag aggaaattgt tcgtcttgtt ggaattgatt ctctgtctga aaatgaacga
     1501 ctaacgatgg aaattgctaa acaaattaga gaagattatt tgcaacagaa cgcttttgat
     1561 tcggtagata cattcacttc gtttgcaaaa caagaagcaa tgttaagtaa tatcctaact
     1621 tttgctgatc aggcaaatca tgctttagag ttgggttctt actttacaga gattatagaa
     1681 ggtaccgtgg cagttcgaga ccgtatggcg agaagtaaat atgtttcaga agatagatta
     1741 gatgaaatca aaattatttc aaatgagatt acacatcaaa ttcaattgat attagaaaca
     1801 ggaggtctat aaatgagtgt tataaaagaa tatagaactg ctagtgaagt tgttgggcct
     1861 cttatgattg ttgaacaagt taataatgtg tcttacaatg agttagttga aattcaactt
     1921 cataatggag aaattcgtcg tggacaagtt ttagagatcc acgaagataa agcaatggtt
     1981 cagctttttg aaggatctag tggaataaat ttagaaaagt ctaaaattcg ttttgctggt
     2041 catgcactag aattggctgt atctgaggat atggttggta gaatttttaa tgggatggga
     2101 aaaccaattg atggtggacc agatttaatt ccagagaaat atttagatat tgatggtcaa
     2161 gctattaatc ctgtatctag agattatcca gatgaattta ttcagacagg gatctcctct
     2221 attgatcatt tgaatactct tgtacgtggt caaaaattac cagtattttc aggttcgggc
     2281 ttacctcata atgaattagc agctcagata gcaagacaag cgactgtttt aaattctgat
     2341 gaaaattttg cggttgtatt tgcagcaatg ggtattactt ttgaagaagc tgagttcttt
     2401 atggaagaac tcagaaaaac aggagcgatc gatcgttcag tcttatttat gaacttggca
     2461 aatgatcctg caattgagcg tattgcaact ccccgcattg ctttaactgc ggcagagtat
     2521 ctagcttttg aaaaagatat gcatgttcta gttatcatga cggatatgac taactattgt
     2581 gaagcgttac gtgaagtctc ggcagctcgc cgtgaagttc caggtagacg aggctatccg
     2641 ggatatttat atacaaattt atcaactcta tacgaaaggg ctggtcgctt agttggtaaa
     2701 aaaggttcag tgacacagat tcctatttta acaatgccag aagatgacat aacacatcca
     2761 attcctgatt taactggata cattactgaa gggcaaatta ttttgtcgca tgagttgtat
     2821 aatcaaggtt atcgtccacc aatcaatgtt ttaccttctc tctctcgatt aaaagataag
     2881 ggatctggag aaggtaaaac tcgtggagat catgctccaa ctatgaatca actgtttgca
     2941 gcctatgccc aagggaaaaa ggttgaagag ttagcagtag tattaggaga atcggcttta
     3001 tctgatgtag ataaattgta tgtgaagttt acaaagcgtt ttgaagaaga gtacatcaac
     3061 caaggatttt ataaaaatcg aaatattgaa gatacgttga atcttgggtg ggaattacta
     3121 tcaattcttc ctagaacaga gttaaaacgt atcaaagatg atttgcttga taaatactta
     3181 cctttggtag aagtttaatc cagaaatgga gtgattatct atggtgcgtt tgaatgtaaa
     3241 accaactcgt atggaattga ataacttaaa ggaacgtttg aaaacagctg aacgtggaca
     3301 taagttatta aaggataaaa gagatgaatt gatgaggcga tttatttctt tgattcgtga
     3361 gaataatcaa cttcggaaag aagtagaaag ttatctaatt gataatctaa aagcctttgc
     3421 agttgctaaa tcattaaaga actctctaat ggtggaggaa ttattttcaa ttccatcgaa
     3481 agaaattgaa ttatttgttg agaaagaaaa tatcatgagt gtaacagttc ctagaatgca
     3541 tatgaatatt acttctcaaa atgagaacag tgaatacagc tatttatctt ctaatagtga
     3601 aatggatgat gtatttgcta caatgaatag tttaattgat aaattactaa gactggcaga
     3661 agttgaaaaa acgtgtcagt taatggctga tgaaatagaa aaaacacgta gacgtgtaaa
     3721 tggattagaa tattcgatta ttccaaactt gtcagaaact attcattata tagaattgaa
     3781 actagaggag gcagaaagag ccaatttagt tcggattatg aaagtgaagt agatccttta
     3841 tttagatttt aattagatga accaatatca gcttggataa ggcggtaagc ctttctaagc
     3901 ttttttattg acagtatcag gatatctttt tcaaaatttt ggtttgttag ataatgaaaa
     3961 tgtttctacc gatttagatt tagg
//
LOCUS       DAVLTM010000134         3834 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLTM010000134 DAVLTM010000000
VERSION     DAVLTM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.135"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..408
                     /locus_tag="VYN16_001883"
     CDS             <1..408
                     /locus_tag="VYN16_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1404368.1"
                     /translation="KPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYKPS
                     TDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLTGLK
                     SSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
     gene            619..3765
                     /gene="phtE"
                     /locus_tag="VYN16_001884"
     CDS             619..3765
                     /gene="phtE"
                     /locus_tag="VYN16_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1404369.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKKLSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEEPKTSEKVEKEK
                     LPETGNSTSDSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVI
                     KKNMADFTGEAPQGNGENKPSETGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENS
                     TDNGMLNPEGNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIE
                     LRLPSGEVIKKNLSDLIA"
BASE COUNT         1437 a          629 c          755 g         1013 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagat aaggaacatg atgaagtaag tgagccaact
       61 caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc agataatctt
      121 tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac tacagatgag
      181 gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga ggcggaggcc
      241 ttgctagaaa aagtaacaga ttctagtatt agacaaaatg ctatggagac attgactggt
      301 ctaaaaagta gtcttcttct cggaacgaaa gataataata ctatttcagc agaagtagat
      361 agtctcttgg ctttgttaaa agaaagtcaa ccggctccta tacagtagta aaatgaatgg
      421 agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa aaacgtaaca
      481 gaaacttgac aaaagtaatt ttaaatagta gactatttac tggttaatta aatggttaaa
      541 taatcggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaaa cttcatcatt
      601 tattgaaatg agggatttat gaaatttagt aaaaaatata tagcagctgg atcagctgtt
      661 atcgtatcct tgagtctatg tgcctatgca ctaaaccagc atcgttcgca ggaaaataag
      721 gacaataatc gtgtctctta tgtggatggc agccagtcaa gtcagaaaag tgaaaacttg
      781 acaccagacc aggttagcca gaaagaagga attcaggctg agcaaattgt aatcaaaatt
      841 acagatcagg gctatgtaac gtcacacggt gaccactatc attactataa tgggaaagtt
      901 ccttatgatg ccctctttag tgaagaactc ttgatgaagg atccaaacta tcaacttaaa
      961 gacgctgata ttgtcaatga agtcaagggt ggttatatca tcaaggtcga tggaaaatat
     1021 tatgtctacc tgaaagatgc agctcatgct gataatgttc gaactaaaga tgaaatcaat
     1081 cgtcaaaaac aagaacatgt caaagataat gagaaggtta actctaatgt tgctgtagca
     1141 aggtctcagg gacgatatac gacaaatgat ggttatgtct ttaatccagc tgatattatc
     1201 gaagatacgg gtaatgctta tatcgttcct catggaggtc actatcacta cattcccaaa
     1261 agcgatttat ctgctagtga attagcagca gctaaagcac atctggctgg aaaaaatatg
     1321 caaccgagtc agttaagcta ttcttcaaca gctagtgaca ataacacgca atctgtagca
     1381 aaaggatcaa ctagcaagcc agcaaataaa tctgaaaatc tccagagtct tttgaaggaa
     1441 ctctatgatt cacctagcgc ccaacgttac agtgaatcag atggcctggt ctttgaccct
     1501 gctaagatta tcagtcgtac accaaatgga gttgcgattc cgcatggcga ccattaccac
     1561 tttattcctt acagcaagct ttctgcctta gaagaaaaga ttgccagaat ggtgcctatc
     1621 agtggaactg gttctacagt ttctacaaat gcaaaaccta atgaagtagt gtctagtcta
     1681 ggcagtcttt caagcaatcc ttcttcttta acgacaagta agaaactctc ttcagcatct
     1741 gatggttata tttttaatcc aaaagatatc gttgaagaaa cggctacagc ttatattgta
     1801 agacatggtg atcatttcca ttacattcca aaatcaaatc aaattgggca accgactctt
     1861 ccaaacaata gtctagcaac accttctcca tctcttccaa tcaatccagg aacttcacat
     1921 gagaaacatg aagaagatgg atacggattt gatgctaatc gtattatcgc tgaagatgaa
     1981 tcaggttttg tcatgagtca cggagaccac aatcattatt tcttcaagaa ggacttgaca
     2041 gaagagcaaa ttaaggctgc gcaaaaacat ttagaggaag ttaaaactag tcataatgga
     2101 ttagattctt tgtcatctca tgaacaggat tatccaggta atgccaaaga aatgaaagat
     2161 ttagataaaa aaatcgaaga aaaaattgct ggcattatga aacaatatgg tgtcaaacgt
     2221 gaaagtattg tcgtgaataa agaaaaaaat gcgattattt atccgcatgg agatcaccat
     2281 catgcagatc cgattgatga acataaaccg gttggaattg gtcattctca cagtaactat
     2341 gaactgttta aacccgaaga aggagttgct aaaaaagaag ggaataaagt ttatactgga
     2401 gaagaattaa cgaatgttgt taatttgtta aaaaatagta cgtttaataa tcaaaacttt
     2461 actctagcca atggtcaaaa acgcgtttct tttagttttc cgcctgaatt ggaaaaaaaa
     2521 ttaggtatca atatgctagt aaaattaata acaccagatg gaaaagtatt ggagaaagta
     2581 tctggtaaag tatttggaga aggagtaggg aatattgcaa actttgaatt agatcaacct
     2641 tatttaccag gacaaacatt taagtatact atcgcttcaa aagattatcc agaagtaagt
     2701 tatgatggta catttacagt tccaacctct ttagcttaca aaatggccag tcaaacgatt
     2761 ttctatcctt tccatgcagg ggatacttat ttaagagtga accctcaatt tgcagtgcct
     2821 aaaggaactg atgctttagt cagagtgttt gatgaatttc atggaaatgc ttatttagaa
     2881 aataactata aagttggtga aattaaatta ccgattccga aattaaacca aggaacaacc
     2941 agaacggccg gaaataaaat tcctgtaacc ttcatggcaa atgcttattt ggacaatcaa
     3001 tcgacttata ttgtggaagt acctatcttg gaaaaagaaa atcaaactga taaaccaagt
     3061 attctaccac aatttaaaag gaataaagca caagaaaact caaaacttga tgaaaaggta
     3121 gaagaaccaa agactagtga gaaggtagaa gaaccaaaga ctagtgagaa ggtagaaaaa
     3181 gaaaagcttc ctgaaactgg gaacagtact agtgattcaa cgttagaaga agttcctaca
     3241 gtggatcctg tacaagaaaa agtagcaaaa tttgctgaaa gttatgggat gaagctagaa
     3301 aatgtcttgt ttaatatgga cggaacaatt gaattatatt taccatcggg agaagtcatt
     3361 aaaaagaata tggcagattt tacaggagaa gcacctcaag gaaatggtga aaataaacca
     3421 tctgaaactg gaaaagtatc tactggaaca gttgagaacc aaccaacaga aaataaacca
     3481 gcagattctt taccagaggc accaaacgaa aaacctgtaa aaccagaaaa ctcaacggat
     3541 aatggaatgt tgaatccaga agggaatgtg gggagtgacc ctatgttaga tccagcatta
     3601 gaggaagctc cagcagtaga tcctgtacaa gaaaaattag aaaaatttac agctagttac
     3661 ggattaggct tagatagtgt tatattcaat atggatggaa cgattgaatt aagattgcca
     3721 agtggagaag tgataaaaaa gaatttatct gatctcatag cgtaaggaat agcagtagaa
     3781 aaagtctgaa tcaaaaatga agttctctca aaagttagaa ataaaactct gact
//
LOCUS       DAVLTM010000135         3745 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLTM010000135 DAVLTM010000000
VERSION     DAVLTM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.136"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            57..275
                     /gene="nrdH"
                     /locus_tag="VYN16_001885"
     CDS             57..275
                     /gene="nrdH"
                     /locus_tag="VYN16_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1404370.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            356..2515
                     /gene="nrdE"
                     /locus_tag="VYN16_001886"
     CDS             356..2515
                     /gene="nrdE"
                     /locus_tag="VYN16_001886"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1404371.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGECYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2703..3665
                     /gene="nrdF"
                     /locus_tag="VYN16_001887"
     CDS             2703..3665
                     /gene="nrdF"
                     /locus_tag="VYN16_001887"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1404372.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1159 a          808 c          702 g         1076 t
ORIGIN      
        1 aaattttatt ttctgatata ctaagatagt attaaatttt gaagaggagt tacacaatgg
       61 taaccgttta ttctaaaaac aattgtgtcc aatgtaaaat gaccaagcgt ttcttggaca
      121 gtaataatgt ctcttatcgt gaaatcaatc ttgatgagca acctgagtac gtcgatcaag
      181 ttaaagagct cggttttagc gcagctcctg ttatccaaac accaactgaa gtcttttcag
      241 gtttccaacc aggaaaactg aaacaattag cataatctta gtacatcatc cagaagaaac
      301 tacttttagg gctaacttag aagcctttct tttgtaatta gataagggaa attttatggg
      361 attaaaacat cttgaagacg tgacttactt ccgtcttaat aacgaaatta accgtcctgt
      421 taatggacaa atcatgcttc ataaagataa ggaagccttg gatgctttct ttaaagaaaa
      481 tgtagttcca aacactatgg tttttgattc aatcaaagac aaaatcaatt acctcattga
      541 acacaactac atcgaaacag cctttatcaa gaaataccgt ccagagttct tggaagaatt
      601 ggctcaattt atcaaagacc aaaacttcca attcaagtca ttcatggcag cttataaatt
      661 ttacaatcaa tatgccttaa agactaatga cggtgaatgc tatcttgaaa atatggaaga
      721 ccgtgtcttc ttcaacgccc tttatttcgc tgatgggaat gaagctgttg caatcgatat
      781 tgccaatgaa atcatccacc aacgctacca acctgctact ccttccttct tgaatgctgg
      841 acgtgctcgt cgtggggagt tggtatcttg tttcctaatc caagtgacgg atgatatgaa
      901 ctctatcggg cgttctatca actcagctct tcaactttca cgtattggtg gtggtgtggg
      961 aattaccctc agcaaccttc gtgaagctgg tgcacctatc aaaggctatg aaggagctgc
     1021 ttcaggtgtc gttcctgtta tgaagctttt tgaagacagc ttctcttact ccaaccaatt
     1081 ggggcaacgt caaggtgctg gtgttgtcta cctcaacgtc tttcacccag atatcatcgc
     1141 tttcctttca actaagaaag aaaacgctga tgaaaaagta cgtgtcaaga ctctatcact
     1201 tggtgttgta gtacctgata aattctacga attggctcgt aaaaatgaag aaatgtacct
     1261 cttcagccct tactctgtag aaaaagaata cggtgtgcca ttcaattaca tcgacattac
     1321 tgaaaaatac gatgaattgg tcgcaaatcc aaatattcgc aagacaaaaa tcaaggcgcg
     1381 ggatttggaa actgaaatct ctaaattgca acaagagtct ggctatcctt atgtagtcaa
     1441 catcgatacg gctaaccgtg caaatcctgt tgatggaaag attatcatga gtaacttgtg
     1501 ttctgagatt cttcaagttc aagaaccaag cttgatcaac gatgctcaag aattccttca
     1561 aatgggaaca gatgtttcat gtaacctggg ttcaaccaac gtggttaaca tgatgacttc
     1621 acctgatttt ggtcgttcta tccgtgctat ggttcgtgcc cttactttcg ttacagatag
     1681 ttcacatatc gtagctgtac caacgattga ccatggaaat agccaagccc acacctttgg
     1741 tctcggtgcc atgggactgc acagctacct tgcccaacaa ctcattgaat atggttcacc
     1801 tgagtctgtt gaatttacaa gcatctactt tatgcttatg aactactgga ccttggtaga
     1861 gtcaaacaat attgcacgtg aacgtggtat taccttccac aactttgaaa aatcagacta
     1921 tgctaacgga agttactttg acaagtatgt aactggtgaa tttgttccaa catcagaccg
     1981 tgttaaagaa ctcttcaaaa atgtctttat ccctggtgtt gctgattggg ctgaacttcg
     2041 cgacaaggtt caagaagatg gtctttacca ccaaaatcgc cttgctgtag cgccaaatgg
     2101 ttctatcagc tatatcaacg acgtttctgc ttctatccac ccgattacgc aacgtatcga
     2161 agaacgccaa gagaagaaaa ttggtaaaat ctactaccct gctgctggct tgtctacaga
     2221 aaccattcct tactacactt ctgcctacga tatggatatg cgtaaagtca tcgatgttta
     2281 cgctgctgcg actgaacacg tggatcaagg actttcactc accctcttca tgcgcagtga
     2341 cattccaaaa ggcctttacg aatggaagag ggaaaataaa caaacgacac gtgacttgtc
     2401 catccttcgt aactatgcct ttaacaaggg aatcaagtct atctactacg tccgtacctt
     2461 tacagatgac ggtggagaag tcggcgccaa ccaatgtgaa agctgtgtga tttagtttct
     2521 agcatagaaa cactattttt ccctaagcag cactgataaa atcaaatcta ctatgtcata
     2581 acataattta tgtaacattt taaaagtcgg gcttccgact ttttgtgcga tactcctata
     2641 gaattatggt aaagtagaaa taattgtgag ttcgcaatac taaacacaga aagagatttt
     2701 aaatggaaac ttactacaaa gccattaact ggaatgccat cgaagatgtc atcgacaaat
     2761 caacttggga aaaactgacg gagcaatttt ggctcgatac acgtattccc ttatcaaacg
     2821 acttggatga ctggagaaag ctatctaaca aagagaaaga cttggtcgga aaagtttttg
     2881 gtggtttaac ccttcttgac actatgcaat ctgaaactgg ggttcaagcc cttcgcgcag
     2941 acatccgtac accacatgag gaagctgttt tcaataacat ccaatttatg gaatctgtcc
     3001 acgctaaatc ttactcatca atcttttcta ccttgaatac taaggctgag attgaagaaa
     3061 ttttcgaatg gaccaatacc aatccttacc tacaaaagaa ggctgagatt gtcaacgaaa
     3121 tctacctaaa cggcagccca cttgaaaaga aagttgccag cgtcttcctc gaaaccttcc
     3181 tcttctactc tggtttcttc actcccctct actatctcgg taacaacaaa ctagccaacg
     3241 ttgcagaaat cattaaattg attattcgtg acgagtctgt tcacggaacc tacattggtt
     3301 acaaattcca acttggtttc aatgaattgc ctgaagaaga gcaagaaaaa ctcaaagaat
     3361 ggatgtacga cctactctat actctttatg aaaatgaaga aggctacaca gagagtcttt
     3421 atgacggtgt tggttggact gaagaggtta aaaccttcct tcgctacaat gccaataaag
     3481 ctctcatgaa catgggacaa gatccacttt tcccagattc agctgaagat gtcaacccaa
     3541 tcgttatgaa cggtatttca acaggaactt ccaaccatga cttcttctct caagtcggaa
     3601 atggttacct tcttggcgaa gttgaagcca tgcaagatga tgactacaac tacggtttgg
     3661 actaatactc ttcgaaaatc aaattcaaac cacgtcagct tcgccttgcc gtactcaagt
     3721 acagcctgcg gctagcttcc tagtt
//
LOCUS       DAVLTM010000136         3724 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLTM010000136 DAVLTM010000000
VERSION     DAVLTM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.137"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(122..655)
                     /locus_tag="VYN16_001888"
     CDS             complement(122..655)
                     /locus_tag="VYN16_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1404373.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(744..1520)
                     /gene="recX"
                     /locus_tag="VYN16_001889"
     CDS             complement(744..1520)
                     /gene="recX"
                     /locus_tag="VYN16_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1404374.1"
                     /translation="MKITKLEKKKRLYLMELDNVDKCYITEDTIVRFMLSRDKVISKE
                     ELTEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYTIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASVLREYL"
     gene            complement(1530..2306)
                     /locus_tag="VYN16_001890"
     CDS             complement(1530..2306)
                     /locus_tag="VYN16_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HEV1404375.1"
                     /translation="MNSKTPRFSILDFPEQLRSYIKGANLSDSSSHSSATVLYIDSGY
                     YLKIDQKGRLAQEAKITSWFETEGLGTPVITYLSANKDFLLTKEAIGSNALAFLNQPE
                     KLCQTLAQALNKLHSLKPQSFPSENHLKRYKEKALKNYQKGTFYNKTLLPQFHIHSRE
                     EAYQLIQEKGYILKADAFIHGDACLPNFILKDASHFSCFIDLGLAGFSDRHIDLFWAI
                     WSLHYNLQDATYAELFLDYYGREYVNMDKLRLVAAFEAFR"
     gene            2363..3718
                     /gene="rlmD"
                     /locus_tag="VYN16_001891"
     CDS             2363..3718
                     /gene="rlmD"
                     /locus_tag="VYN16_001891"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1404376.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1165 a          667 c          697 g         1195 t
ORIGIN      
        1 ttctttctag taatgtgttg taactctgct ataatggatt tattcctttt tgtgtttaca
       61 caatttattt tatactacca aaaaaacaag gtcaggattt tgttcctgac ctttgacaac
      121 tttaccgatt ctttagttct acatagcgct tgtaccaaat gtttacatag gcttctgaga
      181 aaggaccacg tccattgtta atccaatcaa caagaatttt gacatgttct tttaaaatat
      241 agtccaagtc atcagaataa ttcattttgc gtttgtgacg ctcgtattct tcaacgtcca
      301 agagacgttt ttccccatct gtaaaaattt taacatccaa atcgtaatca atatacttca
      361 gtgcttcttc atccagatag taggggctag ccatattgca atagtaagaa gttccattat
      421 cacgaatcat ggcaatgata ttaaaccaat atttcttgtg aaagtaaaca atagccggtt
      481 ctcgagtgac ccaacgacga ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa
      541 taatggcgtt ttctgttgtt tttagtacca tggtgtcccg ccaagttcgg tggagactcc
      601 catcatgctt ataactttga attgtaataa agtcgccttc ttttggaagc ttcataacta
      661 accaactttc tacaatttat aagtttatca tttactattg taccataaaa ttacctaaaa
      721 tctgtgaatt tcacttggaa atattaaaga tattctctaa gaacgcttgc tatatccgaa
      781 aaatcgtagc cctttcgtgc taaaacttga gttaaacgct gcttcagttc gtatccttca
      841 tactttcggg catacttagt atattgctta tcaagttcct tgaagatgag ttcctgagtc
      901 gtttcttggt caacttggct atctaagtcg tcaaaggcaa ttttagcatc agaataagag
      961 aagcccttgt tagtcaagtt ctggataatc ttatcttgca gggcacgagc tggaagtttt
     1021 ccctcatatt ttttcaatag tttattagct acacgttgag caacttcaga aaaatcaaat
     1081 tctttcaagt tctcttctat agtagatttt gaaatccctt tttgagctag tttctgagtc
     1141 agcacataag gacccttgtc tcctgaaagt tgattggtat tgatgatagt ataagcgtac
     1201 tgaccatcat taatccactt atcttcttta agattagcaa taacttgaga aacgatgttt
     1261 tcatcaatat cgtatttttt cagatattct ctgacttctt tttcagtgcg tgctttaaag
     1321 gataagtggt agagggccag attcttacca taagaaaact gggcaaaatc ctgaatctcg
     1381 gtcagttcct ctttactaat gaccttatct ctcgataaca taaaacgaac aattgtgtct
     1441 tcggtgatat agcatttgtc gacattatca agctccatca gatagagtct ttttttcttt
     1501 tcaagttttg tgattttcat aatttccttt catctaaatg cttcgaaagc tgcaacaagt
     1561 cggagtttat ccatatttac atattctcta ccgtaatagt cgagaaataa ttcagcatac
     1621 gtagcatctt gtaggttgta atgaagggac caaattgccc aaaagaggtc gatatgtcgg
     1681 tcactaaaac cagccaatcc caagtcaata aagcaagaaa aatgagaggc atccttcaag
     1741 atgaaatttg gcaggcaggc atctccatga ataaaagcat cagctttgag aatgtaacct
     1801 ttctcttgta tcagttggta agcttcttca cgactgtgaa tgtgaaattg aggcagaaga
     1861 gtcttgttat aaaaagtccc cttctgatag ttttttaagg ctttttcttt ataacgcttc
     1921 agatgatttt ccgacggaaa agattgtggt ttaagactgt gaagtttgtt taatgcttga
     1981 gccagggttt gacagagttt ttcgggttga tttaaaaaag cgagagcgtt gctgccaatg
     2041 gcttctttag tcagaaggaa gtccttattt gctgataaat aggtaatcac tggagttccc
     2101 aatccttcag tttcgaacca acttgtaatc ttagcttctt gagctaatct gcctttttga
     2161 tctattttta aatagtaacc tgaatctata tataggacag ttgcacttga atgagaagaa
     2221 ctatcagaga gattagctcc cttgatatag cttcgtaatt gttcagggaa atctagtata
     2281 ctaaagcgag gtgtttttga attcatgctt ttattgtaac atattcttaa atatcttaga
     2341 aactccatgg tataatagac atatgaatct gaaagtgaaa caaaaaatac cattaaaaat
     2401 caagcgcatg ggaattaacg gtgagggaat cggcttttac caaaaaacat tagtctttgt
     2461 accaggagct ctcaaaggcg aagatatcta ttgtcagatt acttctatta gacgcaactt
     2521 tgttgaagca aaattactga aggtcaacaa gaagtctaaa tttcgaattg tgccatcttg
     2581 tactatttat aatgaatgcg gaggctgcca aatcatgcac ctgcattatg ataagcagct
     2641 ggagttcaag acggacttac ttcatcaagc gctgaaaaaa tttgctcctg caggatatga
     2701 aaattatgaa attcgtccaa ctattggaat gcaggaacca aaatattaca gagctaagtt
     2761 acaatttcag actcgaaaat ttaaaaatca ggtcaaggcg ggcttatatg cacaaaactc
     2821 tcactattta gtagagttga aagactgcct ggtacaagat aaggaaaccc aagtgattgc
     2881 taatcgctta gcagaattac ttacttatca ccagattcca atcacggatg agagaaaagt
     2941 tctaggtgtc cgtactatta tggtccgacg cgcgagaaag accggacagg ttcagattat
     3001 tattgttaca aaccgccagc ttaatttaac tcaattggta aaagagttgg ttaaagattt
     3061 cccagaagtt gtgacagtag ctgttaatac aaatacagct aaaaccagtg agatatatgg
     3121 tgaaaagaca gagattatct gggggcaaga gagtattcaa gaaggtgtac tcaattatga
     3181 attttcacta tcccctcgag ctttttatca actaaatcct gagcaaacag aagtcctcta
     3241 tagcgaagca gtaaaagcgc tggatgttga taaagaagac catttgattg acgcttattg
     3301 tggagttgga acgattggat ttgcctttgc aaagaaagta aaaacactca gaggtatgga
     3361 tattattcca gaagctattg aagatgccaa gcgaaatgct aaaagaatgg gatttgacaa
     3421 tactcattat gaagctggaa cggcagaaga gattattcct cgttggtaca aggaaggcta
     3481 ccgagcagat gctttgattg ttgacccacc acgtacaggt ctggatgata agttattaga
     3541 tactattctt acttatgtac cagaaaaaat ggtttatatt tcttgtaatg tttcgacctt
     3601 ggctcgtgat ttggtacgct tagtagaagt ctatgatctt cattatatcc agtcggtcga
     3661 tatgttccca catacagctc gaactgaagc tgttgtaaaa ttaataaaaa aagtttaaaa
     3721 aagg
//
LOCUS       DAVLTM010000137         3718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLTM010000137 DAVLTM010000000
VERSION     DAVLTM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.138"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1789..3170
                     /locus_tag="VYN16_001892"
                     /pseudo
     CDS             1789..3170
                     /locus_tag="VYN16_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            3151..3354
                     /locus_tag="VYN16_001893"
     CDS             3151..3354
                     /locus_tag="VYN16_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404377.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            3357..3707
                     /locus_tag="VYN16_001894"
     CDS             3357..3707
                     /locus_tag="VYN16_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404378.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWALNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
BASE COUNT         1245 a          661 c          853 g          959 t
ORIGIN      
        1 ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ccggctctat ccgtcaggaa
       61 atcttgaacg caaccaagga taaagcagat aagactttag ttgtatctga agccgggaaa
      121 ttgcgtgaag aattttcaaa aatgaaggtg ggaggccgga atctatggat aaaatccaag
      181 acggttggag ctgtaattga aaaattacct gaaaaccacg tcacaggtca aaaagaatgc
      241 tataggctag agaacaactc tactttaacg ttcaaccttg aaccagattt cagctcaagg
      301 ttgtaccaaa aagttacttt tagcgcttgg atcaagtacg aaaatgtagt ccaaggtcga
      361 aatttttgga atgtatttaa ttgcttcaaa cattatcttt ttagaaaaaa tagtgagacc
      421 ggagtacaga gtggtccaga ttatgctacg cttggtatgt ataaaggttc ggcagattgg
      481 aaatatatta cattcactta tgactactct gaaaaaacaa attttgatca attgaagaca
      541 tcattgcgat tcaatcttga aggtgctaca agcggtacag cttgggtaac aggaatcaag
      601 gttgaaatcg gtagtgtggc gacggactgg agtcctgcgc ctgaggacgc tgatggtctc
      661 attactgaag ctaaggctac ctttgagcgg acagctcagg gcttgcgaac cgacttatca
      721 gctattcagg aatatgtaaa taaagacggt cagcgacagg aagccctaca gcgctatact
      781 cgtgaggaga gcacgagaca agcgacagca gtccgtgagc tggtcaatcg tgatttcgtt
      841 ggtaaggcta cttatcaaga agatgttaag ggtatcaatc agaggattga agctgttaaa
      901 actagtgcga ataaagacat cgctagtcaa atcgctagct atcgtcaatc tgtagatggt
      961 aagttcacgg atatttcaag tcagataact acttataagc aagatgtggg cggtcaaatc
     1021 agtggccttt caaatagact tacaagcagt gagcaaggaa ccactactca gatttcaaat
     1081 ctttcaaatc ggataaacag taataaacaa ggcgcagata atcagatttc aaatttaaag
     1141 actcaggtcg ctacaaacaa ggataatgct gaacgacaaa tgggtagaat atctgatcag
     1201 gtttcggcaa acaaagcgaa tgctgatagt caatttgcga atgtgaccaa tcaactagcg
     1261 cgaaaagtag agactactga ctttcagcgt gttaaggaaa ccagtaaact ttacgagcgg
     1321 attttgggca atactgaaaa tggaattgcg gataaggttg ctcgcatggc tctgaccaat
     1381 caactgtttc aggttgaggt tgggaaatat agtgtaagcg gccctaacct cattaagaat
     1441 agtgatttta aaaattctac gaatgaatgg ggctcaactc aaaatttagg aagattggtt
     1501 aagcatagct tttatcacaa cgggcagaaa gaccttatgc gtttaagtaa tgcaactaaa
     1561 aacgaaaatt ttttgtatag tcaccgtttt aatcttgaac gaaatactga ctatgtactg
     1621 aattttagag gatttaacaa cagtgctcta gcaaactatg atgtttatat tttgggacga
     1681 agagcaggcg agagcgatgg attcacaatc gttaagaaag ttgttagcag caagaaacta
     1741 tctacctcta gatgcgaaga tgtctcagta acttttaatt ccggagaaat ggataatgct
     1801 tacattcgtt ttgataacaa tggctcatca tcaggaacag ctgatttgta tattacagaa
     1861 gttgacttgt acaaaggtta taaacctaga acatggcaac cacatccaga agatgcagtc
     1921 gcagatgcga ataagaagct tgaagcaacg caaacaaaaa tgactcaact agctggctca
     1981 tgggtagttg aaaacatcaa ctcggctgga gatatcatct ctggaatcaa tcttggcgcc
     2041 aatggacata accgcttcgt tgggaaattg acccacatca ctggagagac cctgattgac
     2101 agagcagtca tcaagtctgc catggttgat aagctcaaaa cggccaattt tgaagctggt
     2161 tcggtcacga ctacgatatt agacgctgaa gcggtaactg ctgagaagtt gaaagttgac
     2221 aatgcgctta ttagaaaatt aactgcaaat gatgctttta ttgaccaact gatatctaaa
     2281 cgtatcttct ctactaaggt tgagtccgtc atttctagct caaccttcct agaagcctat
     2341 caaggccgaa tcggtggatt cacacttggt caatttgacc agggtggcgg tcgctggatt
     2401 tcaggtgtca atcagttctc tgttggtatg gggaatggtg ccggatatgg agtccggaca
     2461 gccttctggg cgaactgggg aaataattgg aactatgccg gacctaaagc atggaacgtc
     2521 aatactgatg ggaaaatgta ctgtaggaat gaagtcggtt tttatgatca agtggatttt
     2581 tcgaattcat cgagagcaaa cttctatggg aatactactt tttctcgttc tcctgtgttt
     2641 tcaaatggta tcgaacttgg aagtaaagat gtgcttggtg atggttggaa tcccaaaggc
     2701 ggaaggaatg cggttgtttg gtggaatcag gtcggtagcg gtagcttgaa gtattggatg
     2761 gaacaaaaat cagacagacg cttaaaagag aacatcacag atacagctgt gaaagccttg
     2821 gataaaatca acagattaag aatggttgca tttgatttca tcgaaaataa gaaacatgag
     2881 gagattggtc taatagctca agaggctgaa accatcgttc caagaattgt ctcacgagat
     2941 cctgagaatc cagatggcta tctgcatatc gactataccg ctttagttcc ttacttaatc
     3001 aaggctattc aagaattaaa tcaaaaaata gaaaaaatgg agaaaacaat agcatgaata
     3061 acaacatgtt gaccaatatc gcacttaaag caatttagga gcttgctctt gaaaatagaa
     3121 aacgaacaca cagattggag aacttagaaa atgaacacag aacagcttaa ccaagcctta
     3181 caaatgacaa ttcgtgaaat gtcaacaact tcaacagatt cgatgattac aagtaatatc
     3241 ttgagtattc agttgaatga gcaaagggaa gagaatcaaa gacttcaagc acgagtggat
     3301 gagctggaag ctctgcttga tgaacaaact aaaccagcag acaaaggaga atagacatgg
     3361 cagaaacaat tcaaaacaca gataacttac tagaccttac aaaaatcaca gaaccatttg
     3421 atcttgcgag cgctttgcgc tacatgaaag aaaatggaga attcattcgt tgcaagaatg
     3481 taagcgatga cttctatatg tatcgtgatg ttcaaaaacg tcctgtgatc gtaaatggcc
     3541 gtcgccaatt caaggatgtt gaaaccgttt gggcgctcaa ccagtggggt ggtacaatcg
     3601 caacaatcaa cgtagccgtt ctgttgaatc atgaattcta tatcatgaaa tttgatgcag
     3661 agggcaatcc tgactggacg gttccaacgg tagaacctaa agaataggag gttgtatg
//
LOCUS       DAVLTM010000138         3713 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLTM010000138 DAVLTM010000000
VERSION     DAVLTM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.139"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            55..726
                     /locus_tag="VYN16_001895"
     CDS             55..726
                     /locus_tag="VYN16_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1404379.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            710..1528
                     /locus_tag="VYN16_001896"
     CDS             710..1528
                     /locus_tag="VYN16_001896"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1404380.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            1525..2421
                     /locus_tag="VYN16_001897"
     CDS             1525..2421
                     /locus_tag="VYN16_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1404381.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            2465..3439
                     /gene="pta"
                     /locus_tag="VYN16_001898"
     CDS             2465..3439
                     /gene="pta"
                     /locus_tag="VYN16_001898"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1404382.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         1107 a          685 c          826 g         1095 t
ORIGIN      
        1 ggagaaaaaa tcaaagatat ttttgacgga taatatgaga acaagggaga atatatgacc
       61 ttagaatggg aagaatttct agatccttac attcaagctg ttggtgagtt aaagattaaa
      121 cttcgtggta ttcgtaagca atatcgtaag caaaataagc attctccaat tgagtttgtg
      181 accggtcgag tcaagccaat tgagagcatc aaagaaaaaa tggctcgtcg tggcattact
      241 tatgcgacct tggaacacga tttgcaggat attgctggct tacgtgtgat ggttcagttt
      301 gtagatgacg tcaaggaagt agtggatatt ttgcacaagc gtcaggatat gcgaatcata
      361 caggagcgag attacattac tcatagaaaa gcatcaggct atcgttccta tcatgtggta
      421 gtagaatata cggttgatac catcaatgga gctaagacta ttttggcaga aattcaaatt
      481 cgtactttgg ccatgaattt ctgggcaacg atagaacatt ctctcaacta caagtaccaa
      541 ggggatttcc cagatgagat taagaagcga ctggaaatta cagctagaat cgcccatcag
      601 ttggatgaag aaatgggtga aattcgtgat gatatccaag aagcccaggc actttttgat
      661 cctttgagta gaaaattaaa tgacggtgta ggaaacagtg acgatacaga tgaagaatac
      721 aggtaaacga attgatctga tagccaatag aaaaccgcag agtcaaaggg ttttgtatga
      781 attgcgagat cgtttgaaga gaaatcagtt tatactcaat gataccaatc cggatattgt
      841 catttccatt ggcggggatg gtatgctctt gtcggccttt cataagtacg aaaatcagct
      901 tgagaaggtc cgctttatcg gtcttcacac tggacatttg ggcttctata cagattatcg
      961 tgattttgag ttggacaagc tagtgactaa tttgcagcta gatactgggg caagggtttc
     1021 ttaccctgtt ctgaatgtga aggtctttct tgaaaatggt gaagttaaga ttttcagagc
     1081 actcaacgaa gccagcatcc gcaggtctga tcgaaccatg gtggcagata ttgtaataaa
     1141 tggtgttccc tttgaacgtt ttcgtggaga cgggctaaca gtttcgacac cgactggtag
     1201 tactgcctat aacaagtctc ttggcggtgc tgttttacac cctaccattg aagctttgca
     1261 attaacggaa attgccagcc ttaataatcg tgtctatcga acactgggct cttccattat
     1321 tgtgcctaag aaggataaga ttgaacttat tccaacaaga aacgattatc atactatttc
     1381 ggttgacaat agcgtttatt ctttccgtaa tattgagcgt attgagtatc aaatcgccca
     1441 tcataagatt cactttgtcg cgactcctag ccataccagt ttctggaacc gtgttaagga
     1501 tgcctttatc ggtgaggtgg atgaatgagg tttgaattta tcgcagatga acatgtcaag
     1561 gttaagacct tcttaaaaaa gcacgaggtt tctaagggat tgctggccaa gattaagttt
     1621 cgaggtggag ctattctggt caataatcaa ccgcaaaatg caacgtatct attggacgtt
     1681 ggagactacg ttaccattga cattcccgct gagaaaggct ttgaaacctt ggaggctatt
     1741 gagcttccat tagatattct ctatgaggat gaccactttc tagtcttgaa taaaccctat
     1801 ggagtggctt ctattcctag tgttaatcac tctaatacca ttgccaattt tatcaagggt
     1861 tactatgtca agcaaaatta tgaaaatcag caggttcaca ttgttaccag actagatagg
     1921 gacacttctg gcttgatgct ctttgccaag cacggttatg cccatgcacg attagacaag
     1981 cagttgcaga agaaatctat cgagaaacgc tactttgctt tggttaaggg agatggacat
     2041 ttggagccag aaggggaaat tattgctccg attgcgcgtg atgaagattc cattattacc
     2101 agacgagtgg ctaaaggcgg aaagtatgcc catacttcat acaagattgt agcttcttat
     2161 ggaaatattc acttggtcta tattcacctg cacactggtc gaacccatca aatccgagtc
     2221 catttttctc atatcggttt tcctttgctg ggagatgatt tgtatggtgg tagtctggaa
     2281 gatggtattc aacgtcaggc tctgcattgc cattacctat ccttttatca tccattttta
     2341 gagcaagact tgcagttaga aagtcccttg ccggatgatt tcagtaacct tattacccag
     2401 ttatcaacta atactctata aaaactgtct cagagtataa ttattatctt aaaggagaaa
     2461 actcatggaa gtttttgaaa gtctcaaagc caaccttgtt ggtaaaaatg ctcgtatcgt
     2521 tctccctgaa ggggaagagc cacgtattct tcaagcgact aaacgtttgg taaaagaaac
     2581 agaagtgatt cctgttttgt taggaaatcc tgaaaaaatt aaaatttatc ttgaaattga
     2641 agggatcatg gatggttatg aggtcatcga ccctcaacat tatcctcaat ttgaagaaat
     2701 ggtttctgcc ttggtggagc gtcgcaaggg caaaatgact gaagaagatg tacgcaaggt
     2761 tttggttgaa gatgtcaact actttggtgt gatgttggtt tacttgggct tggttgatgg
     2821 aatggtgtca ggagcgattc actcaacagc ttcaacagtt cgtccagctc tacaaatcat
     2881 caaaactcgt ccaaatgtaa ctcgtacttc aggagccttc ctcatggttc gtggtacgga
     2941 acgttaccta tttggagact gtgccattaa catcaatcca gatgcagaag ccttggctga
     3001 aattgccatc aactcagcaa tcacagctaa gatgtttggc atcgaaccta aaattgccat
     3061 gttgagctat tctactaaag gttcagggtt tggtgaaagc gttgataagg tcgttgaagc
     3121 aactaaaatt gctcacgact tgcgtcctga ccttgaaatc gatggtgagt tgcaatttga
     3181 tgcagccttt gttcctgaaa ctgcagctct gaaagctcct ggaagtacgg tagctggtca
     3241 agcaaatgtc ttcatcttcc caggtatcga ggcaggaaat atcggttaca agatggctga
     3301 acgcctgggt ggctttgcgg ctgtaggacc tgttttgcaa ggtttaaaca agccagttaa
     3361 tgatctttct cgtggatgta atgcagatga tgtttacaag ttgaccctca tcacagcagc
     3421 tcaagcagtt catcaatagt gaaaactata aagcgatata ctatgctata ctgtagttat
     3481 gaaactatgt acgaaaagca ctgccattaa ttcctgagaa ctaaattact gattggtgtc
     3541 aaaaaggaaa acttccaagc gatgatatcc tgtctataca cgacctatag aaatctgtaa
     3601 tatgtatgtc cgtaaaacga taatttccct ttttgatttt aaatgagtat gaaaagagaa
     3661 ttttttcatg agaaaacgca taatattaag tgtcttgaca cctgctatta tgc
//
LOCUS       DAVLTM010000139         3659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLTM010000139 DAVLTM010000000
VERSION     DAVLTM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.140"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            133..2286
                     /gene="blpA"
                     /locus_tag="VYN16_001899"
     CDS             133..2286
                     /gene="blpA"
                     /locus_tag="VYN16_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEV1404383.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2297..3658
                     /locus_tag="VYN16_001900"
     CDS             2297..3658
                     /locus_tag="VYN16_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1404384.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
BASE COUNT         1217 a          738 c          594 g         1110 t
ORIGIN      
        1 atgtttaaaa aaaataattt ctaatcactt ttttaccatt caggaagttt taatgactat
       61 tcaagatttc ataaaatatg aacttagttt tatggcataa tatacctatc cactatatga
      121 aaggaattgc caatgacttc ttataaacgt acatttgttc ctcaaataga tgcgagagac
      181 tgtggtgtcg ctgccttagc ctcgattgct aaattctatg gttcagattt ttctctagct
      241 cacttgagag aacttgcaaa gaccaataaa gaagggacga ctgctcttgg cattgtaaaa
      301 gccgctgatg aaatgggctt tgaaacaagg cctgttcaag cagataaaac tctctttgac
      361 atgagtgatg tcccctatcc atttatcgtt cacgttaaca aagaaggaaa actccaacat
      421 tactatgttg tctatcaaac aaagaaagac tatctggtta ttggtgatcc tgacccttct
      481 gtaaaaatca ctaaaatgtc aaaagaacgc tttttctctg aatggactgg agtagctatt
      541 tttctagctc ccaaacccag ctatcaaccc cataaagata aaaagaatgg tctactaagc
      601 ttccttcctc tgattttcaa acaaaaatct ctcattgctt acattgttct ctcaagctta
      661 ttggtcacta ttatcaatat aggtggttct tattatctcc aaggaatctt agatgaatac
      721 atcccaaatc agatgaaatc aactttagga atcatctcag ttggtctggt tatcacctat
      781 atcctccaac aagtcatgag cttctccaga gattatctcc taaccgttct gagccagaga
      841 ttaagcattg atgtgatttt atcctatatt cgccatattt ttgaacttcc catgtctttc
      901 tttgcgacac gtcgtacagg agaaatcatt tcacggttca cagatgctaa ctctattata
      961 gatgccttgg cttctaccat tctttctctt ttcctggatg tttctattct gattcttgta
     1021 gggggcgtct tactggcaca aaaccctaat ctcttccttc tttctcttat ttctattcct
     1081 atatacatgt tcatcatctt ttcttttatg aagcctttcg aaaaaatgaa ccatgatgtc
     1141 atgcaaagta attctatggt tagctctgcc attatcgaag atatcaacgg gattgaaact
     1201 ataaagtcgc tcacgagtga agaaaatcgc tatcaaaata tagacagcga atttgtagat
     1261 tatttggaaa aatcctttaa gctcagtaaa tattctattt tacaaacaag tttaaagcag
     1321 ggaacaaaat tagttctgaa tatccttatc ctatggtttg gcgctcaatt agtcatgtcg
     1381 agtaaaattt ctatcggtca gctgattacc tttaacacac ttttttctta ctttacaact
     1441 cctatggaaa atattatcaa cctccaaacc aaactccaat ctgcgaaggt tgctaataac
     1501 cgtttgaacg aagtctatct agtcgaatct gaatttcaag ttcaagaaaa ccctgttcat
     1561 tcacattttt tgatgggcga tattgaattt gatgaccttt cttataagta tggttttgga
     1621 cgagatacct taacagatat taatctcacg attaaacaag gagataaggt tagcctagtt
     1681 ggagttagtg gttctggtaa aacaacttta gccaaaatga ttgtcaattt ctttgaaccc
     1741 tacaaaggac atatttccat caatcatcag gatattaaaa acattgataa aaaagtcttg
     1801 cgccgtcata ttaattacct accacaacaa gcctatatct ttaatggctc tatcttggaa
     1861 aacttaactt tgggcggtaa tcatatgatt agccaagaag atattctaag agcttgtgaa
     1921 ttagctgaaa ttcgtcaaga cattgaaaga atgcctatgg gctatcaaac tcagctctct
     1981 gatggagctg gtctatcagg aggacaaaag caacgaatag ccctcgctcg tgctctttta
     2041 actaaatctc ctgttttaat actagacgaa gctactagcg gtcttgatgt cttgactgag
     2101 aaaaaggtta tagataatct tatgtctcta actgataaaa ccattctctt tgtagcccat
     2161 cgtctcagta tagccgaacg aaccaaccgt gtcattgttc ttgaccaggg gaaaatcatt
     2221 gaagttggta gtcaccaaga gttaatgcag gcgcaaggtt tctaccatca tctattcaat
     2281 aaataaggag aatgtcatga atcctaatct ttttagaagc gtcgagtttt atcagagacg
     2341 ttaccataac tatgcgacag tgttaattat acctctttca ttactattta ctttcatctt
     2401 gattttctcc cttgttgcca caaaagaaat tactgttact tcccaaggag aaatcgcccc
     2461 tacaagtgtc attgcctcca ttcagtcaac cagtgataat cctatcctag ctaatcattt
     2521 agtggcaaat caagtagttg aaaaagggga cttactcatc aaatactctg aaacaatgga
     2581 agaaagtcag aaaactgcct tagcaactca attacaaaga cttgagaagc aaaaagaagg
     2641 acttggaatt ttgaaacaaa gcttagaaaa agcgactgat cttttttctg gcgaggatga
     2701 atttggctac cataatacct ttatgaattt tactaaacaa tcccatgata ttgaactggg
     2761 tatcacaaag actaacaccg aagtttcaaa tcaagctaat ctttccaata gcagttcatc
     2821 agctattgaa caagaaatta caaaagttca acaacaaatt ggagaatatc aagagttgag
     2881 agatgctatc ataaataaca gagcacgctt accaactggc aatccgcacc agtcaatttt
     2941 gaatcgttat cttgtagcct cacaaggaca aacacaagga actgcagagg agccattttt
     3001 atctcaaatt aatcaaagta ttgcaggtct tgaatcatct atcgcaagcc tcaaaattca
     3061 gcaagctggt atcggaagtg tagcaactta tgataacagt ttagcaacca aaattgaagt
     3121 actccgcact cagtttttac agacagcctc acagcaacaa ctaactgtgg agaatcaatt
     3181 aacagaatta aaagtacaac tagatcaagc cacacagcgt ttggaaaaca ataccttaac
     3241 ctccccaagt aaaggtatcg ttcatctgaa cagcgaattt gaaggtaaaa atagaattcc
     3301 aactggtaca gaaattgctc aaatattccc tgtcatcaca gatacaagag aagtactaat
     3361 cacttactac gtatcttctg actatctacc tctactagat aaaggacaaa ctgtaagatt
     3421 aaaactggag aagattggaa atcacggcac caccatcatc ggccaacttc agacaattga
     3481 tcaaactcct accagaacag agcaaggaaa tctctttaaa ttaaccgctc ttgcaaaact
     3541 atctaacgag gatagtaaac tcatccaata tggcttacaa ggtcgcgtca ctagtgtaac
     3601 tacaaagaaa acatattttg attatttcaa agataaaatt ttaacacatt ctgattaat
//
LOCUS       DAVLTM010000140         3643 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLTM010000140 DAVLTM010000000
VERSION     DAVLTM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.141"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            108..2432
                     /gene="pflB"
                     /locus_tag="VYN16_001901"
     CDS             108..2432
                     /gene="pflB"
                     /locus_tag="VYN16_001901"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1404385.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(2800..3018)
                     /locus_tag="VYN16_001902"
     CDS             complement(2800..3018)
                     /locus_tag="VYN16_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404386.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3114..3362)
                     /locus_tag="VYN16_001903"
     CDS             complement(3114..3362)
                     /locus_tag="VYN16_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404387.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            complement(3355..3639)
                     /locus_tag="VYN16_001904"
     CDS             complement(3355..3639)
                     /locus_tag="VYN16_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404388.1"
                     /translation="MVWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         1145 a          728 c          685 g         1085 t
ORIGIN      
        1 aaagtgtaaa aatagggatt ttgcacttgt tgaaatcggt tactttatgg tatacttgtc
       61 ttatgaatgt aacagatgac tgttactaga aaaaagagga cattaatatg gttgttaaga
      121 cagttgttga agcacaagat atttttgaca aagcttggga aggcttcaaa ggcgtagatt
      181 ggaaagaaaa agcaagtgta tcacgctttg tacaagctaa ctacacacct tatgatggag
      241 acgaaagctt ccttgcagga ccaacagagc gttcacttca catcaagaaa attgtagaag
      301 aaactaaagc acactacgaa gaaactcgtt tcccaatgga cactcgtcca acatctatcg
      361 ctgatatccc tgctggattt atcgacaaag aaaatgaagt tatctttggt atccaaaatg
      421 atgaactctt caaattgaac ttcatgccaa aaggtggtat ccgtatggct gaaactactt
      481 taaaagaaaa tggatacgaa ccagacccag ctgttcacga aatcttcact aaatatgtaa
      541 caacagttaa cgacggtatt ttccgtgcct acacttcaaa tattcgtcgc gctcgtcacg
      601 cacacactgt aactggtctt ccagatgcat actcacgcgg acgtatcatc ggtgtttacg
      661 cacgtcttgc tctttacggt gcagactact tgatgcaaga aaaagtaaac gactggaatg
      721 caatcaaaga aatcgatgaa gaaacaatcc gtcttcgtga agaagtaaac cttcaatacc
      781 aagcattgca acaagttgtt cgcctgggtg acctttacgg ggttgatgtt cgcaaaccag
      841 cgatgaacgt gaaagaagca atccaatggg ttaacattgc tttcatggct gtctgccgtg
      901 tgattaacgg tgctgctaca tctctaggtc gtgtaccaat cgtattggac atctttgcag
      961 aacgtgacct tgctcgtggt acatttactg aatcagaaat ccaagaattc gttgatgatt
     1021 tcgttatgaa acttcgtaca gttaaatttg ctcgtacaaa agcttatgac caattgtact
     1081 caggtgaccc aacctttatc acaacttcta tggctggtat gggtaacgac ggtcgtcacc
     1141 gtgttactaa gatggactac cgtttcttga acactcttga caacatcggt aactcaccag
     1201 aaccaaactt gacagttctt tggactgaca aattgccata caacttccgt cgctactgta
     1261 tgcacatgag ccacaaacac tcttctatcc aatacgaagg tgtaacaaca atggctaaag
     1321 acggatatgg tgaaatgagc tgtatctcat gctgtgtgtc tccacttgat ccagaaaatg
     1381 aagaacaacg ccacaacatc cagtacttcg gtgctcgtgt aaacgttctt aaagcccttc
     1441 ttactggttt gaatggtggt tacgacgatg ttcacaaaga ctacaaagta tttgatatcg
     1501 aaccaatccg tgacgaagtt cttgaattcg aatcagttaa agcgaacttt gaaaaatctc
     1561 ttgactggtt gactgacact tacgtagatg ccttgaacat catccactac atgactgata
     1621 ggtacaacta cgaagctgtt caaatggcct tcttgccaac taaacaacgt gccaacatgg
     1681 gattcggtat ctgtggattt gctaacactg ttgatacatt gtcagctatc aaatacgcta
     1741 cagttaaacc aatccgtgac gaagatggct acatctacga ttacgaaaca atcggtgact
     1801 acccacgctg gggtgaagat gacccacgtt caaacgaatt ggcagaatgg ttgatcgaag
     1861 cctacacaac tcgtctacgt agccacaaac tatacaaaga cgcagaagct acagtatcac
     1921 ttttgacaat cacatctaac gttgcttact ctaaacaaac tggtaactca ccagttcaca
     1981 aaggtgtata cctcaacgaa gatggttctg tgaacttgtc taaacttgaa ttcttctcac
     2041 caggtgctaa cccatctaac aaagctaaag gtggttggtt gcaaaacttg aactcacttt
     2101 ctagccttga ctttagttat gcagctgacg gtatctcatt gactacacaa gtatcacctc
     2161 gcgctcttgg taagactcgt gatgaacaag ttgataactt ggtaacaatc cttgatggtt
     2221 acttcgaaaa cggtggacaa cacgttaact tgaacgttat ggacttgaac gatgtttacg
     2281 aaaagatcat gtcaggcgaa gacgttatcg tacgtatctc tggatactgt gtaaacacta
     2341 aatacctcac tccagaacaa aaaactgaat tgacacaacg tgtcttccac gaagttcttt
     2401 caatggatga cgccttggat gcattgagct aatcaagttc ttgaataatg aaaaggaacc
     2461 ctcggtcaaa cgactgaggg ttttgtgctt gggatagtgt gagcaattcc ttcggcgcaa
     2521 tatgcaatgt ttttgggaag gaatagtgga ccgaatctga aatataatag attgaaacta
     2581 gaatagtaca cctttatttc taaaacattc ttagaaatcg atttgactgt cctgatcgat
     2641 ttgtcctatt cttatttcat tttactatag tccactgaaa cgtctcaaac agcgctagaa
     2701 attgactgga attctctaag cgatttgttc agattttatt tcatttcatc ataaaaaaca
     2761 agacgactgg ctaattgaca atcatcttgt ttgatactgt taataaaatt cgcgaagttt
     2821 atcttgattt tctttaaaaa atttttctaa ccaatccata ccataagcat aactaaaacc
     2881 ttctaataga acagagaggt caatagtatc aaatcccatg tcttttaaat atttaaaggt
     2941 atcgaatggt gaatgaaagt ttttgtattt aataattgca ccaatatctt gcaaatcctg
     3001 ctctctgata ctcatcatct tcattcctaa cacatactca atagggacta ctaaaacggt
     3061 cagattctca aatgagtaca aggattcgca taaacttaag ggaggttgtt tgttcatatt
     3121 tgctttgcaa catgattgtt gagccataac tcttcgtgga tgttaagatt aaactgtttt
     3181 cctacacgag caataatttc attgattttc tgattttggt cataaaaagc atcaacatct
     3241 tgtgtggcac gtaaaccatg atattctaag acataaccat cgacacagat aattgttaaa
     3301 gtcagatttt cctgaattaa ttccttattt agggcttgga agaccggttt cattttatcc
     3361 atgtttgtat ttctccatca atttttgaac attggttaac ttagcaggtt tatttaatga
     3421 aatcacatat ttatgagttg gagtatgctc ttgaaaggca tacttcaagc taattttttg
     3481 aaaacgggtt agttctttgg agtctagcaa cttttctaca tcttggtttg ttaaataaga
     3541 atagtcttca aaaataactt ttcgcaaata gcgaaaccaa tggcttgcgt tgaattgtga
     3601 ttgtttggat gcctgtatca tataatcgta ccaaaccatt gtc
//
LOCUS       DAVLTM010000141         3641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLTM010000141 DAVLTM010000000
VERSION     DAVLTM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.142"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..126
                     /locus_tag="VYN16_001905"
     CDS             <1..126
                     /locus_tag="VYN16_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HEV1404389.1"
                     /translation="AIAHDGESWGKLVSIGFDIIQTDWPLLLYQYLVEKNKKIKK"
     gene            153..1214
                     /locus_tag="VYN16_001906"
     CDS             153..1214
                     /locus_tag="VYN16_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1404390.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNELKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1231..2235
                     /locus_tag="VYN16_001907"
     CDS             1231..2235
                     /locus_tag="VYN16_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404391.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSETQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIEEKVTMYINPRDIMQF
                     "
     gene            2247..>3641
                     /locus_tag="VYN16_001908"
     CDS             2247..>3641
                     /locus_tag="VYN16_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1404392.1"
                     /translation="MRKSLNWKDWLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAAR"
BASE COUNT         1261 a          510 c          664 g         1206 t
ORIGIN      
        1 gctattgctc atgatggcga atcttggggg aaattggttt caataggatt tgatattatt
       61 caaacagatt ggccattatt actttatcaa tatctagttg aaaaaaataa aaaaataaaa
      121 aaataaaaaa ataaaaataa aggagaacta tcatgttcaa aaactttaaa aagattgcat
      181 tcacttcttc actaatttta cttgctgctt gctcaaacaa tgcttcaaat acaaatcagt
      241 ctactgatga aggaagtgtt gataaatctc aagaaattgt aatctataca aattcggctt
      301 ctaatggacg tgcagattgg ttacaaacca aagcatcaga gcaaggatac aatcttcgtt
      361 tggtagatat ttcaggtgga gaactagctg accgtttgat tgcagaaaag aataatgctg
      421 tggctgatat ggtaattgga ttaaataaat tagaatttaa ccgtattaaa gctgaaaaac
      481 ttcttgtaaa atactcacca aaatgggctg atgaagtaga tagctctttg ggagattctg
      541 aagggtatta ttcaccaatt gtaaatcaac cattagtttt gattggtaat aaagatgcta
      601 aaatgccatc cgactggaca gattttactg attcatctta taaaggtaaa tatggtattt
      661 caaaattaag tacaggtaca tctaagaaca tctttgcaag tattgtttcg agatataaag
      721 atgaaaaagg tgagttagga atatctgatg aaggttggaa agttgcaaaa gcttacctta
      781 aaaatgcaca cgtttatgca gaaggtgaag actatatctc ttcaattatg gataataata
      841 atgagctaaa atatagtatg atgtggggat ctggtgttct acaaaatcaa aaagaacgtg
      901 aatataaatt ccaagtaatg tcaccaaaag ttggtgttcc ttatgtaact gaacaagttg
      961 ggattttaaa tacaagtaag aagcaagcat tattaaaaga atttgtagat tggtttggct
     1021 cttcagaact tcaaaaagaa tggtctgata aatttggttc aattccagct aataaaaaag
     1081 ctctagagca agcaaaagat gaactcaaac agtttgctaa tagtgttaaa ccacaagaac
     1141 ttgactggga gtttatcgga aaaaacatcg attcttggat tgaaaaagca cagcttgaat
     1201 ttgttgaaca gtaatagtta ggattaagtg atgattacat ttaaggatat tcaaattaag
     1261 tataatgatt ttattgccat cgataatctc aatttaacaa tcaatgaagg agagtttttc
     1321 acatttttag gaccaagtgg ttgtgggaaa tcaacaactc ttcgtgcact agttggattt
     1381 ttggagcctt cacaggggca aatactagtt gataatcaag atattacgaa tttagatccg
     1441 gaaaaacgcg aaattggtat tgtgtttcaa agttatgcat tattcccaac aatgactgtt
     1501 tttgataata ttgcttttgg tctaaaagtt aaaaaagttt ctcctgaaat tattaaagct
     1561 aaagttcatg aaattgcgaa aaaaattaat atctcagacg agcaactaaa ccgaaatatt
     1621 tctgaattat cagggggaca acaacagcgt gtagctttag cacgtgcatt agtattagaa
     1681 ccaaaaattc tttgtttgga tgaaccgtta tcaaacctag atgccaaact acgagtttcg
     1741 ttgagaaaag aattgaaacg attgcaaaaa gaactaggaa tcactactct ttatgtcaca
     1801 catgatcaag aagaagcatt aacattatca gataggattg ctgtgtttaa taatggattt
     1861 attgagcaag ttggcacgcc gtatgatatt tataatcaat ccgaaactca atttgtctgt
     1921 gattttattg gctcaatcaa taaaattagc caagaagttc ttaaaaaatt acaaggcgag
     1981 aataaatact ttaaagaaac tgaaggatac atacgcttag agcgtgttaa gttagagaaa
     2041 acttcacctc aagatttatc tataacagga gtaattagtg atattgagtt taatggtgta
     2101 cttattaagt ataaagtaga cttgtcgaat agtgaaagta ttcatattat cgaaaagaat
     2161 gatggactat cacgtcatat aatcgaagaa aaagtcacta tgtacattaa tccgcgtgat
     2221 ataatgcaat tttaaggaga ttgtgcatga gaaagagtct aaactggaaa gattggctca
     2281 taagattagg agttttatgg ctaatctttg ccctgattat ttatccaaat attgaacttt
     2341 tgtctggagt gttttataaa aatggttcat ttactctaga ggcattcaat aagattatac
     2401 actctcagag agcgatgagt agtatattta atagctttat tttggcgatt tcaatggtct
     2461 ttactgtaaa tattgttggg acattgattg ttctactcac agagtactgg gatattaggg
     2521 gttcaaaaat acttaaatta ggttatatga gctctctagt atatggagga attgtattag
     2581 cgacaggata taaatttgtt tatggcgcta atggtcttgt aactaaaacg ctctcaagta
     2641 taattccagg tcttgcccct aactggtttc aaggatttgg tgcagtattg tttattatga
     2701 catttgcttg tacctcaaat cacataatgt ttttaacaaa tgcagttcgt agtgttgatt
     2761 atcatacaat tgaagcagct aaaaatatgg gtgcaagtcc tttaaaagtt ttctttagtg
     2821 tggtttttcc aacttttaaa cctacgttat ttgccttaac catcttaact tttttgacag
     2881 gtctttcagc agtttctgct cctttgatag taggggggaa agattttcaa actattaacc
     2941 cattgattat cagtttcgct aaaactacaa gttcgagaga tttagcagct ttactagcta
     3001 tcatgttagg tttagcaact attctattat tgacactttt gaatcatgtc gaaaaaggag
     3061 gtaattatgt atctgtttcg aaaaccaagg ctagtattaa gaagcagata atttcttctc
     3121 cattatggaa tactcttgct cacattgttg cgtatgtatt gtttgcaatc tatatgttac
     3181 caatagtttt gataattcta tactcgttta cagactcgtt gacgatcaag acgggaactt
     3241 tagatctatc taaatttacg ttagcgaact atgctaattt atttacagat gctcaatcat
     3301 ttcgtcctta cctagtcagc gttgtttatg caattttagc tgcggtaatt gctacaatta
     3361 tagcaatagt aatttcacgt attgtacata aaaacaagag taagttcgat aaattctttg
     3421 agtatggtgc actaatacca tggatattgc caggtacatt gattgcacta ggattaatgt
     3481 tcacttataa tatacctcat ttaatactat ttaatttagt tttagtagga acagtaatta
     3541 ttttacttat tgcatatact attcaaaaat taccattttc ttatagaatg attcgggctg
     3601 tatttttcag tatagataat gatatggaag aagcagcacg t
//
LOCUS       DAVLTM010000142         3625 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLTM010000142 DAVLTM010000000
VERSION     DAVLTM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.143"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(149..3046)
                     /gene="nanA"
                     /locus_tag="VYN16_001909"
     CDS             complement(149..3046)
                     /gene="nanA"
                     /locus_tag="VYN16_001909"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1404393.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            complement(3334..3561)
                     /locus_tag="VYN16_001910"
     CDS             complement(3334..3561)
                     /locus_tag="VYN16_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404394.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
BASE COUNT          953 a          835 c          606 g         1231 t
ORIGIN      
        1 tatattttat agaacagact actctgaaag tagtttcaga cctcttatga tttcgtatca
       61 gcctgaatgt catcaaaaaa agatagcagg cttaaaaacc tgctatctcc ttctattttt
      121 acaaaatcaa atgtttagaa ttcttctctt attgttctct ctttttccct agcgtaaaca
      181 gaccaaggaa gaaagctgtt agtcctagtg aagctaggag gtcactctcc ttgtttcctg
      241 tttcaggaag tgcctgctga gcaagaggag ctttgtaagt ataatcttct tttgtagtaa
      301 gagttacaag cgaatcagat cccttatact ctgcgatttc atgaacagct ggctctgtac
      361 cattaactcc ccctgtaaat tctggaactt catgcaccgc tgctttgcta ccatttactc
      421 cgccaggaac tctggcacct gctagattta caggaagatt atgcatactt tcgatgcttc
      481 ctttagctat accaataatt tcctgtccga tatcttcctt atctactgca aacaacaagg
      541 tcttgctatc atactgggtt aggaaagtcg ctgttttacc atttgccaat tgaagtgttg
      601 gagccttgtt gaccaatact tctgagtcga actccaagcc aataactcct ttgcccatct
      661 ctcctttgcc catctctcta gttcgcttca ctttcgcttc ggtaggagaa atcagatttt
      721 tgctcaaaaa ttcccaatta aattttctaa atgatagggt ataggcattt tgtccttttt
      781 cagtatgttc atacaagatg ccatactccc catttcctaa ttcttggagc gaattatagg
      841 caaactctcc tttttgaatt ggattgtgtt tgagccaagt caactcacca ttttcttcga
      901 cacgtgccaa gtggaccatc ccattttcac gtttcggtcc acctgcatta ctgaggatga
      961 tgtattcttt tccttcgtgc atcgtatgga tagcagacat ttgaacatag acatctttaa
     1021 cctgtggata acgtttgata tccttctccc aagtcactcc tccgtcttta cttgtagcaa
     1081 cctgaagatc tccagtcaaa ccacgcataa agagtttaac atctccattg tttagttgta
     1141 ccaccgttga ttctgtattt tgcgcacgtt tattgttcat cgtagaagag tggatctttt
     1201 gaccgtctac ctgacggtta tcgttgaccg cttctccagc atgccaagtt tttccatgat
     1261 catctgaata gatgacacga gaagattgcg agccatttaa gtgagataca ttattagtcg
     1321 tataaaccgg tatcaaaatc cgtcccttgt gaggcccatt ccgaagtaca attcctgttc
     1381 caggacctac acccaagaat ttcatccaat cggctttgac catcggagta atatcttgag
     1441 gcgctgacca tgtcttcccg tcgtcatcac tgtaggacat ccatagatag ctatccttgg
     1501 caattctaaa tggagaagtt ttgtttgttg tgaagtagat atttcctagt aattggtcac
     1561 ccttgtatag atcacccttg tcgctatagg ctggtttaac aggatctaca acaacgcgat
     1621 agtctgtcgc cttaccatct ggtgtataga cagtaccatt ttctcgaatg gtataagctt
     1681 ccttttctcc ctcacgatag aggatttgat aggtttttcc atcgatttct ttgtaggctt
     1741 cttctttttg tgaagacatt ccaaagattc ccttcccttc tgggaacatg tcatagatag
     1801 aaaagattcg tttggtttca ggatcttgaa ccaacaccat atcgatattc actggtgaac
     1861 cgatcgatgg gtcagaagct tttggattgt cacgtaagtt ggtaatggtt actcggtcac
     1921 cccaagtttt accattatct tcactacgtc tgatgaccat accgatatca ccccagtcac
     1981 tcgaatggag acggcgttca tctgcacctg cgatcaaagt tcctttatct gtcttgagaa
     2041 gtgctggaat acgataactc ttgattccat ctttatttgg tttgttgttc attccccctt
     2101 caaagacgtc ttctttctct gagattttcg caccttctgg aagtttcttc tccaaatcac
     2161 ctctttcaaa tagttggctt ctcgtctgaa cttcttctgg gcttaaggca cgattataca
     2221 cagtgagatt ccgaatctgt agatttgacc cccaaaccgt attgttggca cgcttggttg
     2281 ctccgatttg cacatgcgtt acatctggca tatctttaat gaaattgcca gatttcagac
     2341 ttgttcgaga taaaacacca ttaacataca ggcgaactcg accatttggc aattctgctg
     2401 ttggtttttc aactgtgaaa gtcacagaat tccactgacc tggtttaact tttaagggtg
     2461 catcgttgta attattgtaa aactgtttcc catccgaacc acgcccctct agagtagcag
     2521 tattattgta aactgccata gtgaagtact catctttttt agtagcactt gacacagaaa
     2581 agagattata gaatgctgga gccttggcat ctggcttaaa ctccatgtga actgttgcgt
     2641 tttcaagttt ctttagttta tctagttcac ttgataaatc aactctctga ccatttgaag
     2701 cattggtttc aacatcttct ttttctatca ctgtttcgac attttccaaa tctcgtgcat
     2761 agtaatctct tggaattttt tcttcttgct tatctttcct ttcttgttct tgcttattgc
     2821 cagaaagttc agtctctgaa gaaggctggc tcttttctgt atcagttagg gttgagctct
     2881 cccccgaaag ttgagtttca tttgccagag gttgctcact tgccccttct tgagctaaaa
     2941 caggagacgt tccaaatacc actgctccta caatcataga aaccgctcct accgatagtt
     3001 tcctaatgct ataacgacac ttacgttctt gaacactccg attcatactg atcctctcta
     3061 tatctatatc ccgatttctg aaataagaca tttactccag aaaatgcact tagtttgtct
     3121 tttattaact ttcttttact acagttcatt taaatgacaa aaatgaacta aacaaactat
     3181 ctcaaattgg tgccaatttt cccctctcct ttctccaatc agactcctgc caatctagtt
     3241 tatctaccaa gacaccgctg caattaaact aaaatatctc tttataagca aatgatggac
     3301 atcagcttat gctatttaca ccatacttcc tgctcaatcc ttatcgaaca ctttttcaaa
     3361 tagcttttca taagcataaa tacttgtaga aataatggga aatataatca agctcacaat
     3421 cagaatctta tccagcttaa aggggacaat cccaaataag tgaagaaata aatcaatcag
     3481 aaaactgtcg aaaaaaactg tcaaaagtag ttcaacttgt ctttttctct gcttttttgt
     3541 cttgaatgcc ttaaatatca tattattccc cttttctaag cttcaatccc ttctctctat
     3601 ctgttatttc gcatagctac tacta
//
LOCUS       DAVLTM010000143         3615 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLTM010000143 DAVLTM010000000
VERSION     DAVLTM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.144"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            177..1238
                     /gene="dinB"
                     /locus_tag="VYN16_001911"
     CDS             177..1238
                     /gene="dinB"
                     /locus_tag="VYN16_001911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1404395.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1385..2230)
                     /locus_tag="VYN16_001912"
     CDS             complement(1385..2230)
                     /locus_tag="VYN16_001912"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1404396.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTNRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2486..3436
                     /locus_tag="VYN16_001913"
     CDS             2486..3436
                     /locus_tag="VYN16_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1404397.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQIELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTRYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNI
                     HAFDNPAQLQAFAGLDSSIYQSDQIDLAGRMVKRSSPHLR"
BASE COUNT         1177 a          750 c          709 g          979 t
ORIGIN      
        1 taacactcaa tggaaatcga aaagcaaact aggaagctag ccgcaggctg ctcaaagcac
       61 tgctttgagg ttgtaaatag aactgacgaa gtcagctcaa aatactgttt tgaggttgca
      121 gatggaagct gacgtggttt gaagagattt ttgaaaagta taaaaaggtg ctaggcatgt
      181 tgatttttcc tttgttaaat gatttgtcaa gaaaaatcat ccatattgac atggatgcct
      241 tttttgctgc ggtggaaatc aaggataatc ctaaactcag aggaaaacct gtcattattg
      301 gaagcgaccc tcgacaaaca ggtgggcggg gagtcgtttc tacctgtagc tatgaggctc
      361 gagcttttgg tgtccattct gccatgagtt ccaaggaagc ttatgaacgt tgtccccagg
      421 ccgtctttat ctcaggaaat tatgagaaat acaaagctgt gggactccag attcgagcta
      481 tttttaagcg ctatacagat ttgattgaac ccatgagtat tgacgaagcc tatttggatg
      541 tgacagaaaa taaactcggt atcaagtcag cggtcaaaat tgctcgcctc attcaaaaag
      601 atatctggca agaactccat ctaactgctt ctgccggtat ttcctataac aagttcttag
      661 ctaaaatggc gagtgattat caaaaacctc atggtttgac agtgattcta cctgaacagg
      721 ctgaggattt tctcaaacaa atggatattt ccaaatttca tggagtagga aaaaagacag
      781 tagaacgtct tcatcaaatg ggcgttttta ctggtgctga tttacttgaa gttcctgagg
      841 tgaccctaat agaccgtttt ggtagactag gctatgatct gtatcgaaag gctcgtggca
      901 ttcacaactc tccagtcaaa tccaatcgca tccgtaaatc aatcggcaag gagaaaacct
      961 acgggaagat tctccgtgct gaggaagata tcaaaaaaga gctgactctt ctatcagaaa
     1021 aagtcgctct caatctacat caacaagaaa aagctggaaa aattgtcatt ttgaaaatcc
     1081 gctacgagga cttttcaact cttaccaaac gaaaaagtct tgctcaaaaa acacaagatg
     1141 ctagtcagat aagccaaata gccctgcaac tctatgaaga attaagcgag aaagaaagag
     1201 gtgtccgcct attggggatt accatgactg gattttaaag ctcaatgaaa atcaaagagc
     1261 aaactaggag gctagccgca ggctgctcaa aacactgttt tgaggttgca gataaagctg
     1321 acgcggtttg aagagatttt ctaagagtat aaaaccttga agagttgccc cttcaaggtt
     1381 tttcttatac aaataaacgg acaaaactgt aaagtagcaa aacactacca agcacgatac
     1441 ggtatttacc aaaaagggtg aagtcgtgtt ttttcacata gctggtcaag aagcgaatag
     1501 ccaccatgct gaccgcaaaa gctactccca tcgcgaccaa gagcaaaaac aattgcccaa
     1561 agctcaagag ttctccggct ttcacaaatt tgaaaatctt taaggcacta gctccaaaca
     1621 taacgggaat cccaagatag aaggtaaatt ctgtcacaac tgaacgattg gttccattta
     1681 acaaaccacc gacaatcgtt gcacctgaac ggctagtccc tggtaaaaga gcaagaactt
     1741 ggaagagtcc gatatagaaa gcggtcgtat aaggaagctt gtccaactct gttacacttg
     1801 gctcgatagc acgcgcttta ttgcgctttt ccaaatagat gaaggcaacc ccgtagataa
     1861 tcaacatgag agcaactgaa accatgttat ggaagtgggt atcaaaccaa tcatcaaatt
     1921 taaagacacc aagtaaaggc aaagtggcaa tcaagacctt caaccatagt ctccaagtct
     1981 tacgaacttc ctgtttgtcc ttagtcggtt taaaaggatt gagcttgtta aaataaatca
     2041 ccataactgc taaaatagca ccaagctgaa tcacgacatt aaacatggac ataaaggctt
     2101 cattttgatt ttggtattgg ataaactcct ctgctaaaat caagtgacct gtactggaaa
     2161 tcggcaacca ttccgtaatt ccttcaacaa tcccgaagaa gatagatttt aaaatttcaa
     2221 taagatacat atatttctcc ttatattata tttaacgcag ttagctactg ccaaagccca
     2281 agtggtatac ttggaataag ccactgtgga ttagttcatt tcctccaatc gcttgacgat
     2341 ttccgaaagg gtctaaccac agctttttgt ttcaattggt aatgagtttg ataacgcata
     2401 gacgttttat ctcgtctaag gtaacaaggc aaaaagttcg tggataacca agtagacttg
     2461 aaatactata tctggaggta ctcccatgct ttatgtgggc attgatgtcg ctaaaaataa
     2521 acacgatgtt acagccttga atgttccagg aaaaactgtt cttaaaccac tcactttttc
     2581 aaataataaa gctggttttg aactcttaga tctgtctttt cgacagctca accaagactg
     2641 tctcatcgct cttgaagata ctggacatta tgcctttaat ctactgaact tcctgcatga
     2701 acaaggatat aaagtttaca cctacactcc tttgctcatc aaggaatttg ccaaatcatt
     2761 atcacttcgc aagactaaaa cggataagaa agacgctcat ggcatcgctc ttaaacttct
     2821 ttctgacccc aatcgtgaac aatttcaaca cgataatcgg caaatagaac taaaaatact
     2881 ggctagacat attcatcgtc tcaagaaaaa acagtctgat tggaaagtac aatacactcg
     2941 ttgtcttgat atcatctttc ctgagttgga taaaatcgtt ggaaagcatt cagaatatac
     3001 ctaccaactc ttgacacgct accctaatcc tcagaaaagg cttgaggcag gatttgataa
     3061 gctgatagaa attaagcgat tgaccgcttc taaaattcag gatatcctct cagtcgcacc
     3121 tcgttctatc ggaacaacat ctcctgctcg tgaattcgaa atcatcgaaa tcatcaaaca
     3181 ttacaagagg ctcattgaca aggcggaaac atgtgtcaat gacttgatgg ctgagttcaa
     3241 ctcggtcatc acgacggtta ctgggattgg gggtcgttta ggggcggtca ttttagccga
     3301 gattcgaaat attcatgcct ttgataatcc tgctcaatta caagctttcg ctggactgga
     3361 ttcttctatt tatcagtcag atcagattga tttagcagga agaatggtca aacggagttc
     3421 ccctcatctg cggtaggtac tcatacaagc tgccaaagca tgccctcgct tttcacctgc
     3481 ttttaaggcc tatcttaaga ctaagttaga ataaggaaaa cattacaatg tagccatcat
     3541 ccaccttgca aaaaaactta tccgaaccct gttttatatc ctaaaaaaga gctgccattt
     3601 gacgaacaaa aagtg
//
LOCUS       DAVLTM010000144         3572 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLTM010000144 DAVLTM010000000
VERSION     DAVLTM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.145"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            51..1259
                     /gene="sstT"
                     /locus_tag="VYN16_001914"
                     /pseudo
     CDS             51..1259
                     /gene="sstT"
                     /locus_tag="VYN16_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1327..2280
                     /locus_tag="VYN16_001915"
     CDS             1327..2280
                     /locus_tag="VYN16_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1404398.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2423..2803
                     /locus_tag="VYN16_001916"
     CDS             2423..2803
                     /locus_tag="VYN16_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1404399.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            2902..3123
                     /locus_tag="VYN16_001917"
     CDS             2902..3123
                     /locus_tag="VYN16_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1404400.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3140..3454
                     /gene="trxA"
                     /locus_tag="VYN16_001918"
     CDS             3140..3454
                     /gene="trxA"
                     /locus_tag="VYN16_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1404401.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         1007 a          808 c          694 g         1063 t
ORIGIN      
        1 gtctatcatt tatactataa tcataatcac tacacaaaaa ggagattatt atgaaaagaa
       61 tcattcatgc ttggaataag gcaagccttg tcaagcgtat cttgattggt atgctttgtg
      121 gggaatccta ggactgaccc ttcctaatct ctcagggatt gggctactcg gggatttatt
      181 cgttggaggt ctaaaagctg ttgctcctat cctagtcttt gccctcgttg ccaatgccct
      241 ttccaacatc aaaagggaca agatagcaat atgaaaactg ttgttttctt gtatatccta
      301 tgatagggac tttcgccgct gctcttgtag ctgtactagc aagtttcatc gtccctattg
      361 aaattaccct aaatagtgcc aatactgaaa ttgcaccacc agatgggatt gggcaggtcc
      421 tcagcaacct cttgctcaaa ctggttgaca acccagtcaa cgccctgctt actgctaact
      481 atattagaat cttatcttgg gcagtcattt ttggaatcgc tatgagagaa gccagtaaaa
      541 atagtaaaga attgctaaaa actatcgctg acgtgacttc taaaattgtc gaatggatca
      601 tcaatctggc tccatttgga atccttggtc ttgtttttaa aaccatttct gacaagggag
      661 tcggaagcct tgccaactac ggtattttat tggttctatt agtaacgact atgctttttg
      721 ttgcccctgt ggtcaaccct ttgattgcct tcttctttat gagacgcaat ccttaccctc
      781 tagtttggaa ctgcctccgt gtcagcggtg tgacagcctt tttcactcgt agttctgcga
      841 ctaacattcc tgtcaacatg aaactctgcc atgaccttgg actcaaccca gatacctatt
      901 ctgtttctat cccactcggt tctactatca atatggctgg agtagcgatt accattaacc
      961 ttttgaccct tgctgcagtt aacactcttg gaattcctgt tgactttgcc acagcctttg
     1021 tcctcagtgt ggtagcagct atctcagcct gtggtgcttc aggtattgcc ggaggttccc
     1081 tccttcttat cccagttgct tgtagccttt tcggtatttc taacgatatt gccatacaag
     1141 ttgttggggt tggttttgtg attggtgtca tccaagactc atgtgaaaca gcccttaact
     1201 cttctacaga tgtcctcttt accgccgttg ccgaatacgc agcaacccgt aaaaaataac
     1261 tcatcaaggc aagcctgctc atgtcttgtc ttttacgctt ttattctaac ttattaggaa
     1321 attcttatgt ctattagcca acgtacgacc aagctcatct tagctacctg tcttgcctgc
     1381 ctgcttgctt attttctcaa tctttcgtca gcagtttcgg ctggaattat cgctctcttg
     1441 agcctatctg atacgcgtag aagtacttta aaactggctc gcaatcgtct tttttctatg
     1501 cttctagctc tggctatcgg tgttctagct tttcacttga gcggatttca tatctggagt
     1561 ctcggcctct atctggcctt ctacgttcct ttagcctaca agatgggctg ggaaattggc
     1621 atcacaccaa gcacggtttt ggttagccat ctcttggttc aagagtcaac ctctccagac
     1681 ctcctagtca acgaattcct tctctttgct attggtacag gatttgcctt gcttgttaat
     1741 ctctatatgc cttcacgaga agaggaaatc cagcactacc acacgctggt ggaagaaaag
     1801 ttaaaagata tcctccagcg cttcaaatac tatttatcca gaggagacgg acgcaaccga
     1861 gcacagctgg tagcagaatt agacacgctt ttgaaagaag ccctcagact ggtctatttg
     1921 gatcactctg accacctctt tcaccagacc gactaccata tccactactt tgagatgaga
     1981 caacgacaaa gtcgtatcct gagaaacatg gcccaacaga ttaacacttg tcaccttgcc
     2041 gccagtgaaa gcctgatctt agcgcaactc ttttcaaaaa ttgcaggtca actgagccag
     2101 accaatcctg cttctgattt gctagatgaa attgaacgtt atctggaagt cttccggaac
     2161 cgcagtctgc ccaagacaag agaagagttt gaaacccgcg ccacccttct tcaactccta
     2221 cgtgaagcca aaaccttcat ccaagtaaaa gttgattttt accaaaaata tagacagtaa
     2281 aaaataaaac cactaaaaaa cagagcttgc cagcattctt gaaaagtagt ataattattg
     2341 catgcgcaat catcttgtga cgcagagact gcccgttaat ggactttctt ctatttatga
     2401 ctctaaaaag aaaggagaca ctatgaccta tttggaaaaa tggtttgact tcaatcggcg
     2461 tcagaaagaa attgaaagtc tcttggaaga gaccattgcc caacagagcg agcaaagtct
     2521 gaccttgaaa gagttctacc tgctctacta tctggacttg gctgaagaaa aatctctacg
     2581 ccagattgac ctgccagata agcttcatct gagcccgagc gcagtttcta ggatggtggc
     2641 tcgcttggaa gctaaaaatt gcggtctact cagtcgcatg tgttgccatc aagatagacg
     2701 ctctagtttt atctgcctga caaatgatgg gcaaaagaca ctggcctcgc tacaaaagac
     2761 tgtcgaagaa agcttggaag caggtttgga tttcttgatt taagatgatt ttaaaaaaag
     2821 ttgcgtgcgc aatcattttt cttgacattc tcttttataa ggagtaaaat aaagttagca
     2881 ttaaacaaag gagttttaaa catgattgaa attacctata tagatgccag caagaacgaa
     2941 agaactgtga ctttcgagtc ttatgaagac tttgaacgtt cgcaacaagc ttgccttatc
     3001 ggcgtcgcag actactaccc tgtccaaaaa ttaacttaca agggtcataa tttggactac
     3061 catgggactt atggagatat cttcttctat ctcatgaaac aagatttaag ccaatataac
     3121 taaaaaaagg agaaatacaa tggcaaaagc aattacagat gcaacattcg aacaagaaac
     3181 aaaagacggt ttggtcttag tagacttctg ggcaacttgg tgtggtccat gtcgtatgca
     3241 aggtccaatc ttggacaaat tgtctgaaga actttcagaa gatgtcttga aaatcgttaa
     3301 aatggacgtt gatgaaaatc caaacacagc tcgtgctttt ggaatcatgt ctattccaac
     3361 tcttctcttc aaaaaagacg gccaagttgt caaacaagtt gcaggtgttc acacagcaga
     3421 acaaatcaag gccatcattg ctgaattgag ctaatcaaac gagagaccaa gtactttctt
     3481 tggtctcttt tttcttgccc tttgccattt ttcaaaaaat atgctagact atagcagatt
     3541 gaaactagaa tagtacacct ctacttctaa aa
//
LOCUS       DAVLTM010000145         3531 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLTM010000145 DAVLTM010000000
VERSION     DAVLTM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.146"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(17..394)
                     /locus_tag="VYN16_001919"
     CDS             complement(17..394)
                     /locus_tag="VYN16_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1404402.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKICQREGDTHQSYKHIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            627..1604
                     /locus_tag="VYN16_001920"
     CDS             627..1604
                     /locus_tag="VYN16_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1404403.1"
                     /translation="MLKLGVIGTGAISHHFIEASHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1601..2683
                     /locus_tag="VYN16_001921"
     CDS             1601..2683
                     /locus_tag="VYN16_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1404404.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSKT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLIYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2747..3531)
                     /locus_tag="VYN16_001922"
                     /pseudo
     CDS             complement(2747..3531)
                     /locus_tag="VYN16_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1073 a          771 c          649 g         1038 t
ORIGIN      
        1 ttgtcctgtt ctcatttcat tttactataa aactatgaaa agctaggtca tctgagaagt
       61 aattactatc gacaattgcg atagcgtata ctggtgcgat gtgtttgtag ctctggtggg
      121 tatcaccttc acgttggcaa attttttcta ggttttgatt aacctgactg cacagataag
      181 cccataggcg attgatgaaa aaattctgat gatgaacctg aatttcgatg ataacttgga
      241 ttccattatc taactccgcc aagacgtcta tactagtata gaaatcctgc gctgagtagg
      301 gcatggaagg caagacgtga atgttacttc cctccaaaat cgtcacattt tttgctggca
      361 agtccagcat atcgcggata aattgacaaa tcattagttg ggctgatacc cggatgtctg
      421 agaatcctcc ttttcctcct tctattatag cacattttta aatctaggtt tgctagattc
      481 tatgcttcta tctatttatt cggaaaagaa agcttttctt cagaagaaag ctaatagctt
      541 atttgaaatt ttctcctctc tgttggatag tcccgtctat ctatgttata atgaaagaag
      601 gatttaaaat cggaaaagga gtatttatgc taaaattagg tgtcatcgga actggcgcta
      661 tcagccatca tttcatagaa gcatcccata ccagtggaga ataccaactg gtcgcaatct
      721 attctagaaa actagaaact gcagcaacct ttgcttctcg ctatcaaaat atccaactat
      781 tcgatcaatt agaagtcttc ttcaaatctt cttttgattt agtctatatc gccagtccaa
      841 actccttaca ttttgctcag gcaaaagctg ccttgtctgc tggtaaacat gttattcttg
      901 aaaagccagc tgtcactcaa ccacaagaat ggtttgattt gattcaaaca gctgaaaaaa
      961 ataactgttt tatctttgaa gcagctcgta attatcacga gaaagccttt actactatca
     1021 aaaacttttt agcagataag caagtgttag gagcagattt caactatgcc aaatactctt
     1081 ccaagatgcc tgacttgttg gctggacaaa caccaaatgt cttttcagac cgttttgctg
     1141 gtggagccct catggatttg gggatttatc ccctctatgc tgctgttcgt ctttttggaa
     1201 aagccaatga cgcgacttac catgctcaac agcttgacaa tagcattgac ctaaatggag
     1261 atggtatcct cttctaccca gactatcaag ttcacatcaa ggctggaaaa aacatcactt
     1321 ccaatcttcc ttgcgagatt tatacaacag atggaacctt gactctcaac acgattgagc
     1381 atattcgctc agctattttt accgaccacc aaggaaatca agtccagctt cctatccaac
     1441 aggctcctca tacgatgact gaggaagtcg ctgcatttgc acacatgatc cagcaaccag
     1501 atctgaatct ctaccaaact tggctgtatg atgcaggctc tgttcatgag ctactatata
     1561 ccatgcgcca gactgctggc attagatttg aggcagaaaa atgaaaacca aactaccgac
     1621 tgaatggcaa gaactgagtg accaactcgg tttccaagaa ttcaccccca ttcaaactca
     1681 actatttgag ccccttcttg ctggagaaaa cctcctagga gtgagcaaaa ccggaactgg
     1741 taagacccta gcgtatctcc taccaagtct tctcagacta caaaagaaaa aagcccaaca
     1801 actcttgatt ctagcaccaa atacagaact tgctggacag atttttgatg tatgtaaaac
     1861 gtgggcagaa gctatcggat tgactgctca actcttcctt tcaggttcaa gtcagaaacg
     1921 acaaattgaa cgtcttaaaa aggggccaga aattctgatt ggaactcctg gccgtatctt
     1981 tgaactcatt aaacttaaaa aaatcaagat gatgaatgtg gaaaccatca tcctggatga
     2041 atttgaccaa ttactagatg attctcagat tcactttgta gaaaaaatca ctcactacgc
     2101 acctcgtgac caccaactga tctacatgag tgccacgacc aagtttgacc aagaaaagat
     2161 tgtgccgaat acacgcacta ttgatctctc tgatcaaaaa ttggacaata tccagcattt
     2221 ctacatgcag gtagaccaac gtcatcgagt ggatatgcta cgaaaactgg ctcatgtaga
     2281 ggatttccgt ggtctagtct tcttcaatag cctgtcagac cttgggaatg ccgaggaaaa
     2341 actacagtat cgtgatatat tggctgtttc cctcgctagt gatgttaatg ttaaatttag
     2401 aaaagtcatc ttagaaaagt ttaaagataa ccaactaacc ctgcttcttg caactgacct
     2461 tttggctcgt ggaattgata tcgatagcct agaatgtgtc gtaaactttg atattcctag
     2521 agatagcgaa acctacacac accgtgctgg ccgtactggc cgtatgggca aggaaggata
     2581 tgttattact ctcgtcactc atccagaaga aatcaaaaaa cttaaaaagt ttgcaagtat
     2641 ccgagaaatt gtcctaaaaa accaagaact ctatatcaaa taaagcccca ttgctctata
     2701 cacatccagt gtatggagct tttttataag caattcaatt ttatactcat ctgctttcaa
     2761 aaagcattct agtccatctc cgattaacga tggactttat cacctccttc tccagtcctt
     2821 gtatgacatc ttggagttga ttcatgacat cttccaaagt tcgaaaggct ttattcttaa
     2881 atccacgttt acgaatctct ttccacactt gttcaatggg gttcatctct ggtgtgtacg
     2941 gaggaataaa tgcaaagcca atattagtcg gaatctttaa ggtacttgat ttatgccata
     3001 tagcattgtc tataacgagt aaaaaataat catatggata agcttgtgaa agctcttcta
     3061 aaaagacgtt catccactca gtattacatc caccagctat taagaaaact gatgcgcctg
     3121 tatgggcatc aacagctcca taacaatagc gaaattctcg tatatagtga ctatggatat
     3181 gtggacctac tcctattgga gcccaacaag atcccagttt actgattcta ccgaaaccag
     3241 cctcatcttg gtacatcaag cgaaccttac gaaaacgtct actggtttta aagcgctttc
     3301 ttgtcttctt gaattgagat tttattttta gatgcgacaa tggtttgagc gtctgctttc
     3361 ttaggatgtt ctggacgtgg cgtaatattt cgccaaccat ggcgcttcaa cagttgatag
     3421 aaggcatcac gtgtgtagga acgacctaac tcctttttat aagcctgaaa taaggcatca
     3481 attgtaacaa attctcctgc ctctgcagcc ttcaaatggc gggcaagaaa g
//
LOCUS       DAVLTM010000146         3472 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLTM010000146 DAVLTM010000000
VERSION     DAVLTM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.147"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(48..482)
                     /locus_tag="VYN16_001923"
     CDS             complement(48..482)
                     /locus_tag="VYN16_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1404405.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     EFNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            complement(496..1956)
                     /locus_tag="VYN16_001924"
     CDS             complement(496..1956)
                     /locus_tag="VYN16_001924"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1404406.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            complement(2080..3429)
                     /locus_tag="VYN16_001925"
     CDS             complement(2080..3429)
                     /locus_tag="VYN16_001925"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1404407.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
BASE COUNT          915 a          732 c          675 g         1150 t
ORIGIN      
        1 tattttttca acttatagta aaaaaagcca gaattatact gactcttcta tcgctcatta
       61 aacttagaag cacgttcttc tccccaccaa taagggatta gttctgcgac tttaactgtt
      121 tttcttatat tatagtccat catgaattct gcatctttat tttcagcatt aaactctaaa
      181 aggaattctc tacaagcacc gcaaggcatg gctgaacttc caccataagg tggtttgtct
      241 cgaaaggcta atactttctt aaccttagtt tgtcctgaaa attggtacat attgaagagg
      301 gccgcccgtt ctgcgcagag atggaaaaca ccacaggttc cctccataca gaatcctgta
      361 aatatttgtc catctcctgc ttctactgca gctacaacat gattggcata aacaaagtct
      421 gatacttcat gtggattgta tagtttctgt gcttcttcgt acatcttttc ccagatgtcc
      481 attattgtat cctctttatt tagagatttc ttttagcatg ttttcgatat gctgaattga
      541 tttttcacgt ccaagcaaga aaattgtatc tggtaattct ggcccatgca tttcgcctga
      601 aactgcgata cgaataggca tgaaaagatt tttcccttta atacctgttt ctttttggac
      661 tgctttaatt tgtgggaaga tattttctgt cacaaattca tcatctgtca tcgcttcaag
      721 ttttgctttg aatgcttcaa gaactgttgg aactgtttca cccgtcatga cttcgcgctc
      781 tgcttctgtc aattctggga aatctgagaa gaaaagatct gtcaatggga taatctcatc
      841 tactgatttc atttgtggtt tatagagctc aactaatttt tcagccttgt cagtcaaacg
      901 gcctgcttcc tctaagaatg gttttgccat ttcaaagatg gtttcaaggt ctgcattctt
      961 gatataatca ttgctcatcc agtctagttt tttctgatca aaggctgctg gtgacttgct
     1021 gaggcggttt tcatcaaaaa gtttaatgag ttcttcacga gagaaaatct catcttcacc
     1081 acctgggttc caaccaagaa gagcaataaa gttaaagact gcttctggaa ggtaaccttt
     1141 ctttcggtaa tcttcgataa attgaagtgt attagtatca cgtttagata acttcttacc
     1201 agtctcagag ttgataatca aggtcatgtg accgaactct ggagcttccc aaccaagagc
     1261 ttcatagacc ataagctgtt ttggtgtatt agcaatatgg tcatctccac ggataacatg
     1321 agagatttgc atatcgtggt catcgataac aacggcaaag ttgtaagttg ggtaaccgtc
     1381 tttcttttgg ataacccagt caccaccgat attgccacct tcaaattcga tatcgccttt
     1441 gaccatatca tgccacttgt agatacctga ctcattgaca gccaaacgaa cagttgggat
     1501 gatccctgct gcttcacgtt ctgcgatgta agctgctttt tcttcttcac tcataccaag
     1561 gtattcattg atgtagcgtg gtgtttcgcc agctacttct tggcgttcgc gttcagctgc
     1621 caactcttct tctgtaacgt aagatttata ggcttttcct tcagctaata gttggtcaat
     1681 gtatttttga tacaagtcta aacgctcaga ctggcgataa ttctcatgtg attctggact
     1741 ttcatcccaa tccatgccta accagcgaag gttttcaagt tgtgaacgtt caccatcctc
     1801 gacatggcgt ttacggtcag tatcttcgat acggatgaga aatgttccac catgatggcg
     1861 cgcatacaag taattaaaca atgctgtacg agcatttccg atgtgtagta gtcctgttgg
     1921 acttggtgcg taacgtacgc ggatatcttt tgacatagtt tctcctatta tctaggcgtc
     1981 tgcctttttg ctttctatat tatatcacaa gtcttggctg agtccaattt ctatggataa
     2041 agagagtaaa aagagtgggc aaaccactct tttcttctat tatagacgtg cgttaagttc
     2101 tttgctcaat tcttcaaatc ctggttttcc aagaagggca aacatgttac gtttataagc
     2161 ttcaacacct ggttggtcaa atgggttgat agcattcaag taacctgaaa gggcaattgc
     2221 caattcgaag aagtatatag tgtaaccaag agtgaaagcg tcttgctctg gaagagtcac
     2281 atacatgttt ggtacatcac catctgtgtg ggcaagaaga acaccgtcag ttgctttttt
     2341 gtttacaaag tcaacgtctt ttccttgaag gtaaccaagt ccgtcaaggt cttcttccaa
     2401 agtaggaata agcacgtttt tacgaggttt gtcaacacgg acaactgttt caaacatgat
     2461 acgagttcct tcttggataa attgaccaag tgagtgcaag tcagttgaga agttggctga
     2521 agttgggtag ataccttttt ggtcttttcc ttctgattca ccagccaatt gtttccacca
     2581 ttctgagaag tattgaagtg atggctcata gtttaccaag atctcagttg catagccttt
     2641 acgataaagg atgttacgaa cggctgcgta ttggtaagct tcgttttcag agattttgtc
     2701 tgaagtgtag tctttgcgag ctgcattcgc accttccata agagctttga tgtcagctcc
     2761 tgatgctgcg attggaagca aaccaacggc tgtcaatact gagaagcgtc caccgatatc
     2821 atctggaaca acaaatgttt cccaaccgtt agcgtctgct tcaaccttaa cagcaccctt
     2881 ttggcggtca gttgttgcat agatacgttt gttagcttct tcttgaccgt atttcttaac
     2941 caagagttct ttaaagacac ggaaagcaat cgctggttca gttgttgtac ctgatttaga
     3001 aattacgttt actgagaagt ctttgtctgc aacatactct accaagtcag caaggtaagt
     3061 agatgagatt gagtttcctg cgtaaaggat ttgtggagct ttgcgttctt cttttgtttg
     3121 caagttagca aaatggtgat tcaagaagtc gattgctgct tttgcaccaa ggtaagatcc
     3181 accaataccg atcacaacca aaacatcgct gtctgacttg atttgctcag cagctttcaa
     3241 gatgcggtca aattcttcgc ggtcgtattt ttcaggaagg tccaaccatc ccaagaagtc
     3301 gctaccagca ccagttcctt tacggatcaa ttcatctgct gctgttactt gtgattgcat
     3361 gtattccact tcatgtggtg caacaaattt gtctaaaact tttgaataat caaatttaat
     3421 atgtgacatg ttcttcctcc aatatttctt cttttctatc ataacgctta cc
//
LOCUS       DAVLTM010000147         3370 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLTM010000147 DAVLTM010000000
VERSION     DAVLTM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.148"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            38..787
                     /locus_tag="VYN16_001926"
     CDS             38..787
                     /locus_tag="VYN16_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1404408.1"
                     /translation="MFILIINRPLNDSQSFKTKSNIAQIEAQALKHLDKPIIDLSGWQ
                     RPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKSHITELQKRNVPVA
                     VYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMNEGVENFRAKLASL
                     GAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKTDLDYDIHQYTSKG
                     KIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            882..1724
                     /locus_tag="VYN16_001927"
     CDS             882..1724
                     /locus_tag="VYN16_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1404409.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            1724..2206
                     /locus_tag="VYN16_001928"
     CDS             1724..2206
                     /locus_tag="VYN16_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1404410.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYHIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            2206..2826
                     /locus_tag="VYN16_001929"
     CDS             2206..2826
                     /locus_tag="VYN16_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1404411.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(3079..3174)
                     /locus_tag="VYN16_001930"
                     /pseudo
     CDS             complement(3079..3174)
                     /locus_tag="VYN16_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1021 a          695 c          619 g         1035 t
ORIGIN      
        1 tcatccactt attttattta gttttttggg gattatgatg ttcattttaa tcatcaatcg
       61 cccacttaat gacagccaat cgtttaaaac aaaatccaat atagcacaga ttgaagcaca
      121 ggctttgaaa cacttagaca aaccgattat tgacctctct ggctggcagc gtcctgagga
      181 aatcaactac gatgccctct cacaaaatat ttcaggtgct attgttcgtg ttcacagtgg
      241 tgctcaaacg acaaaagaaa atgatgcttc atttatcaat ggaatagaca aggcctataa
      301 gagtcatatc actgaacttc aaaagcggaa tgtcccagtt gctgtctacg cttatgtagc
      361 tggaaaaagt gcccaagaaa tggaaaaggc tgccgaagtt ttctacaatg ctgcctctcc
      421 ttacagccct agttactatt ggcttgacgt ggaagacaaa accatgtcta atatgaacga
      481 aggtgtcgaa aactttcgag caaaactagc atcactaggt gctaaaaaca tcggcatcta
      541 cgttggtgtc tacttcatgg aagaacacag tattgataca ggcaagttta cctctgtttg
      601 gattccgtcc tacggctcag actcaggatt tttggagagc agtcctaaaa ctgatttaga
      661 ttatgatatt caccaataca catctaaagg aaaaattgcc ggctttgacc acgatttaga
      721 tatcaacgtt atctctccct taaaaaataa agaagaaacc tttcgaaaac tttttttaaa
      781 accttaaaag catttgttta tcatttgctt tttctttttg tgtctgattg tgatacaata
      841 tagtaaggat tttttgaaat agaaatagtt ggaggaaata tatgctctca tggttagcac
      901 gcgttattaa agggattgta attgctcttg gatttatcct accaggaatt tccggagggg
      961 ttctagcagc aatcttagga atctatgaac gaatgattgg ctttctggcc catcccttta
     1021 aagaccttaa agaaaatgtt ttgtacttta ttccagttgc catcggtatg cttctgggaa
     1081 tcggcttatt ttcctacccg attgaatacc tgcttgaaaa ttatcaggtc tttgtattat
     1141 ggagctttgc gggagctatt atcggtacag ttcctagcct cctcaaagaa tcaactcgag
     1201 aatctgaccg agacaagatt gatttagctt ggttatggac aacctttatc atttctggat
     1261 taggactcta tgccttaaat tttgtcgttg gaaccttaag cgccagcttt cttaacttcg
     1321 tcctagcagg cgcactattg gcccttggcg tcttggttcc tggcctcagc ccatcgaatt
     1381 tacttttgat tttgggactc tatgctccta tgttgactgg ttttaaaact tttgatctct
     1441 tgggaacctt ctttccgatt ggaattggtg caggtgcaac tctcatcgtt ttttcaaaat
     1501 tgatggatta tgccttaaac aactaccact cacgcgtcta tcatttcatc atcggtatcg
     1561 tcctatcaag tacccttttg atcttaattc caaatgcagg aaacgctgaa agtatccaat
     1621 acacaggact ttcacttgtc ggttatgtca tcatcgcctt cttctttgcg ctgggaatct
     1681 ggcttggtat ttggatgagt caattggagg ataaatataa ataatggcaa aaaaagttaa
     1741 aatcaaaaaa acattggtgg aacaaatcct atctaaagca gctatccctc atcaggggat
     1801 tcaaatcaat gccctagaag gagagcttcc tcaaggttat gaacgagatc agattttcaa
     1861 aaccttggcg cttttgggag acaagaccgg accgattatc ggaattgtcc ctatcactca
     1921 gcacttgtct gaaaagaaac tagctaaaat ttctggcaat aaaaaagtga gcatgattcc
     1981 acaaaaggac ttagaaaaaa caactggtta cattcatgga gccaataatc ctgtcggaat
     2041 tcgtcagaaa cacaattatc acatttttat cgataaaatt gctctagatt tggatcgaat
     2101 gattgtctct gcaggcgaag tcggacacag cattatcgtc gcgccacaag acttggctag
     2161 ctttgtaaag gcagactttg tagatatttt ggaggacatc aagtaatgaa actctacttt
     2221 gtccgccacg gtcgtaccct ctggaaccaa gaaggtcgtt ttcaaggtgc tagcggtgat
     2281 tctcctcttc ttcctggatc cattgaaacc ctaaaacgac ttggccagta tctcaaggaa
     2341 attccttttg atcagattta ttcaagtgat ttacctcgag cggtcaaatc tgctgagatt
     2401 atccaaagtc aactccatac cccctgttct ttagaaatcg ttcctaatct ccgtgaatgg
     2461 caacttggga agttagaagg tttgaaaatt gcaaccctgg aagctattta cccgcaacaa
     2521 atccaggctt ttcgttcaaa tcttgctcaa tttgacactc gaatgttcgg agccgaatcc
     2581 ctctatagta caactcagcg gaccatccaa tttatcaaat cattaaaaga tagtccagct
     2641 gagcgtattc taattgtcgg acacggcgcc aatcttactg ccagtcttcg tactctccta
     2701 ggctataaag aaccacttct tcgtaaagat ggcggtctag caaatgccag cctgaccatt
     2761 atagaaaccc atgattttga aacattcact ctcaatactt ggaatgatac ttcttatcaa
     2821 tcataattat agcctagcct tctgaactag aaccatagga gatatagtca gttgaaacaa
     2881 aaataagaca aaagaacctt gtaaaaaggt attgcaactt gccaatacct ttttgatatg
     2941 aacacgtgtc ttctcaacag gattgtagag caaaatgaaa taagaactga ataatatgat
     3001 ttgagaattt aaatcaattt ataagaatgt tttagaagca gttatgtact attttagatt
     3061 cacttttcta tactaagatg agtagggagg aaaaggtaaa agtttatgcc caaactcttc
     3121 acataagaac tctagcttac ccattctatg gaatcttgca ttatccatta aaacttccag
     3181 actcataccc ttctaaaatc tattcaaacc acgtcagctt cacattatat atgttggtta
     3241 cctcacaagt tttatctgca acctcaaagc tgttctttga gcagcctaca gctagcttcc
     3301 tagtttaatc tttgcttttc actgagtatc aagttaaaaa atgaggctgg tcaaaaagtc
     3361 tttaaaatta
//
LOCUS       DAVLTM010000148         3329 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLTM010000148 DAVLTM010000000
VERSION     DAVLTM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.149"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(178..789)
                     /gene="rpsD"
                     /locus_tag="VYN16_001931"
     CDS             complement(178..789)
                     /gene="rpsD"
                     /locus_tag="VYN16_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1404412.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(984..2036)
                     /locus_tag="VYN16_001932"
     CDS             complement(984..2036)
                     /locus_tag="VYN16_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1404413.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2033..2731)
                     /locus_tag="VYN16_001933"
     CDS             complement(2033..2731)
                     /locus_tag="VYN16_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1404414.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2898..>3329)
                     /locus_tag="VYN16_001934"
     CDS             complement(2898..>3329)
                     /locus_tag="VYN16_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1404415.1"
                     /translation="GTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTAN
                     GSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSE
                     DKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          925 a          745 c          639 g         1020 t
ORIGIN      
        1 taaaagatta taccacattg tgtactattt ttggttcatt ttactatatc actttggact
       61 atatcggtct agttatgtct aaacgattat agctggatac taagagaaat caaaaaagca
      121 ctaagggaga acgtcccaaa gtgcttaata tcaaagccta tcttattcaa taaaatatta
      181 caacattttg ttgtagaatt caacgacaag tgcttcgttg atttctgggt tgatttcgtc
      241 gcgttctggc aagcgagtca atgaaccttc caatttttca gcgtcgaatg atacgaatgc
      301 tggacgtcca agagtagctt ctactgcttc aaggattgct ggaactttca atgatttttc
      361 acgaactgag atcacttgac ctggagttac gcggtatgat gggatatcaa cgcgtttccc
      421 gtcaacaagg atgtgaccgt ggtttacgaa ttgacgagct tgacgacgag tagtcgcgag
      481 accaagacgg taaacaacgt tatccaaacg acgttccaaa agaagcataa agttgaaacc
      541 taggattccg cctttgattt ttgtagcttg tacgaacaag ttacggaatt gtttttcacc
      601 tacaccgtaa gtgaaacgaa gtttttgttt ttcagccaat tgcaaaccgt attctgacaa
      661 tttagaacgg ttgtttggtc cgtgttgtcc tggtacgtag ttacgacgtg ccaattcttt
      721 acctgtacct gtaagtgaaa ggccaaggcg acgagcttgt ttccaagatg gtcctgtata
      781 acgtgacata tgaatgtcct cctgatataa ataatatttc ggtggaaata gtcacttaga
      841 aagccctgat tcgtgcagat gcccttcgcc taaacagcca aggttacttg tcataagaca
      901 cctgttgacg agcttcatgc tttcctgctg atatttcaca caaaatctat tctaccatga
      961 atagctggat ttgtaaaggg gttttaggct ttattttcac taccagagag gttgagaacg
     1021 agggtaaagg tacttcctag accgtactgg ctgctgactg tgatttcccc acccaattga
     1081 tgggccaatt cacgcgcaat cgcaagtcct aatccatgac cacctgtctt catgttacgc
     1141 gaagtttcga cacgataaag acgtttgaaa atattttcca aatcctctgg ggcaataccc
     1201 tgcccttcat cggtcacact gattgaaagc tggtccttct ccagcttagc caccacttcc
     1261 agcttggttc ctggagcaga atatttaaaa gcgttatcga ccagattcac caagatacga
     1321 gaaagcttag cataatctcc ctcaatccgg gcagactctg ggattacctg caagtggaca
     1381 tctcttctct cctgctcaat caaaaactga aattcactca tgcactcaat taagagcttg
     1441 tccagaaaaa tactgtcttt actggtagtt tccacctgat ttctagctgt gtttagggtc
     1501 aaaaaattca actcctcaac cagtttattg agcctctccg tctggcgtcc aatggttgct
     1561 agataatgag cttgctccga ctccttgata atcccatcca aaatcccttc taccgtcgct
     1621 tgaatcgaag tgataggggt cttaatatca tgcgacaact gggcaatcat caagcccttt
     1681 tctcgttcgc tttcttccaa ggaatcaaag cttacctgca aatcatggga catctcatta
     1741 aaagtttgcc ctaattgctg aaattctaca ggaccttgaa cctccaaatt tgaaggaaaa
     1801 tccttggccg ctacccgctt ggcatgctcc ttgagtttgc ccaacgacgt aaagactggc
     1861 aataggagaa agagactaat cccagcaccg acaaggctgg caacgatggt catcccaagc
     1921 aaaaagtaaa tctcgccttt cgcaatcagc attttttgaa cagcccaaaa aacaaccaaa
     1981 atggttaaga gggttgaaat aatatatcca accaaaatat aactttttag tttcatgttt
     2041 gtcctctcgg tttctctatc ttatatccca acccccaaac tgtcttaata gtgggcgttt
     2101 ggtcactact atattttgcc agctcctgtc gaagagcatg gatatgcaca ttcaaggtat
     2161 tggtgtcatc cacgtagtct tctttccaga tcttttcata gaggtctgtc ttggagaaaa
     2221 ctcgctctgg attgctagct aaaatccaca gcaattcaaa tgatttaaca gttaaatcca
     2281 gcatttcttc tcctatttga acttcatgac tactatgatt catttttaga ttgccaaggg
     2341 aaatcagctc tgtttcgccc ccacgatgaa ggcggcgcaa aatattgtgg acacgcaaaa
     2401 ccagctcacg tgggctaaaa ggcttagcaa taaaatcatc tgctcccaag ctcaggccgt
     2461 aaatcttgtc ctgttcactg gtcttagcag taataaatag gaaaggctgc tctggtgata
     2521 agtattgaac ctcactgatt aaatcataac catccatccg aggcatcatg acatctgtga
     2581 taatcaaatc aatcggtttt ttcttgaaca gctctagcgc ttccagtcca tcatgggcta
     2641 ccaagacctg ataacctgcc tgaattaagt atctctgatg aatatctgtg atttctacct
     2701 cgtcgtcaac gagtaaaatt gtctttccca tctgtctctc ctttgataaa aacagtgcta
     2761 tactgcttta agtataacac tatttttatt gttttacgat gattttaatc tgctattgaa
     2821 agaaagtcct gattgctgat tgagttgtta aactaatctt agtcagccca ctatccatca
     2881 tttatttcct tagctgatta gttttcttct tttttgtttt tcaaacctag accaccgagt
     2941 aaacctgcaa gcgcaagccc aaggaaacca atacttgcca ttgatgtttg agtctcacca
     3001 gtatttggta gcatagcttt atcctctgac atcatcgtat cagacatctt gttagcagaa
     3061 gcagccatgt tttcacctgc catcgtgttg gtagaacttg tcatggtgtc agcaggcatg
     3121 ctatctgtag tacctgtagc atgattgtga ttcatcggag tcacgccaga accagagtta
     3181 gaaggtgata atgaaccatt tgctgtgtct gatgtttctt taacatttat cttaatagtg
     3241 acttttttag ttgctacgat gttgtccaag tctggtttac cgtctttgtt accatagaca
     3301 ttgactgtag cgctgtaagt ttgagtacc
//
LOCUS       DAVLTM010000149         3318 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLTM010000149 DAVLTM010000000
VERSION     DAVLTM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.150"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            32..601
                     /locus_tag="VYN16_001935"
     CDS             32..601
                     /locus_tag="VYN16_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1404416.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQIKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            714..1673
                     /locus_tag="VYN16_001936"
     CDS             714..1673
                     /locus_tag="VYN16_001936"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1404417.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            1683..2798
                     /locus_tag="VYN16_001937"
     CDS             1683..2798
                     /locus_tag="VYN16_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1404418.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            2803..3150
                     /locus_tag="VYN16_001938"
     CDS             2803..3150
                     /locus_tag="VYN16_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1404419.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            3158..>3318
                     /locus_tag="VYN16_001939"
     CDS             3158..>3318
                     /locus_tag="VYN16_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1404420.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGL"
BASE COUNT         1067 a          687 c          643 g          921 t
ORIGIN      
        1 ccgctttcaa cgataaaaaa aggtaagaaa aatgaaacat ttagaaattg aattgaaaac
       61 actattgaaa aaagatgaat acaatcgtct aaaagaccag ttcacaggtg tcactcctgt
      121 tcttcaaaca aattactaca tcgacacgcc tgattttgaa ctgcgagaaa agaaagtcgc
      181 tatgcgcatt cgaacctttg aagactgggc tgagttgaca ctcaaagtcc cgcagagtgt
      241 tggaaacatg gaatacaacc aaaaattgca actaaaagat gctgagaact atctgagtaa
      301 ggaagaactt cctcaagggc tagtactgga tgaattggcg aaacatggta tccaaattaa
      361 gaactggcag gttcttggtt gtctaacaac gcttcgctat gaaatgaaaa cagctattgg
      421 tctcatggca ctggatcaga gccgatactt tgatatgaca gattacgaat tggagcttga
      481 agtggaaaat catgagcaag gcaaacagga tttccgacaa tttttagaga aaaatcagat
      541 ttcctaccaa aaagctcctt caaaattggt tcgatttgtc aaaagtatga aaaatagctg
      601 aaataatctc cattttttgg taaaatagaa aagataaaaa tacacaaact ccagttcata
      661 tatgtaaggt aaatataact aggatttata aagtttacag aggacggtct ataatgtcag
      721 atagaaaaaa catgaaactt ttcgcactca actctaacca agagattgca cagaaaattg
      781 cccaagctgt tggtgtccca cttggaaaac tatcatcacg tcaattttca gacggagaaa
      841 tccaagtaaa tatcgaagaa agtgtccgtg gttatgatgt ttacatcatc caatcaacaa
      901 gtttccctgt caacaaccac ctaatggaat tgttaatcat ggtcgatgct tgtgtgcgtg
      961 caagtgccca cagtatcaac gttgtccttc catattttgg ctatgcacgt caagaccgca
     1021 ttgcttgtcc tcgtgagcca cttacagcaa aactagttgc caatatgctg gttaaggctg
     1081 gagttgatcg tatcctgact cttgatttgc atgccgttca ggttcaaggt ttctttgata
     1141 ttccagtgga taatcttttc actgttcccc tattcgcaaa acattactgc gataaaggat
     1201 tgcttggttc agatgttgtt gtcgttagcc ctaaaaattc aggtgtcaaa cgtgcgcgta
     1261 gcctggctga atatcttgat gctcctatcg ccattatcga ctaccctcaa gacgatgcaa
     1321 ctcgtaacga aggttatatt attggtgatg ttgaaggtaa gaaagctatc ttgattgatg
     1381 atattttaaa tacaggacgt accttctctg aagcttctaa aatcgttgaa cgtgaaggag
     1441 ctacagaaat ttatgctgtt tctagccacg gtctcttcgt cgagggagct gctgagcttc
     1501 ttgacaatac taatattaaa gaaattcttg tgactgattc agtagcaaca aaagaaaaaa
     1561 ctcctaaaaa tgtatgctac atcactgcta gtgagttaat tggtgatgct atcgtccgta
     1621 ttcacgaaag aaaaccagtc agcccactct ttgcctacaa taaaaagaaa taaggtgatt
     1681 ctttgattta tttggacaat gctgcaacga ctcctatgtc agcagttgct atttcagcta
     1741 tgaccaaggt tatgcaagaa acccacggaa atccttctag tattcatggt catggtcgtc
     1801 aagctggcaa actcttgcga gaagcccgtc aggaactagc ccagttacta aggacaaaac
     1861 ctcaacatat ctttttcact tctggtggga ctgaaggcaa taatactacc atcattggct
     1921 actgtcttcg tcaccaagaa caaggaaaac atatcatcac aactgccatc gagcaccatg
     1981 ctgtccttga aacaattgat tacttggttc aacactttgg gtttgaagca accattatcc
     2041 agccagaaaa tcaagaaatc acagcccagc aaattcaaaa ggctttacgt gacgatacga
     2101 ttttggtttc taccatgttt gccaataatg agacaggaaa cctactgccc atcgctgaaa
     2161 ttggccaaat actcaagcaa caccctgctg cctatcatgt tgatgcagtt caggctattg
     2221 gtaaaatccc aattcattca gaagaattgg gcattgattt tctcactgct tctgcccaca
     2281 aattccatgg tcctaaggga atcggttttc tctacgcatc tagcatggac tttgattcct
     2341 atctacatgg cggagaccag gaacagaaaa aacgtgcagg aactgaaaat ctgcctgcta
     2401 tcgtaggcat ggttgcagcc ctaaaagaag acctagaaaa acaagaagaa cattttcaac
     2461 atgtacaaaa tctagaaact gcctttctgg cagagctgga gggcattcag tattacttga
     2521 atagaggaaa acatcatctc ccttatgttc tcaatattgg atttcctggt cagaaaaatg
     2581 acctcttact ccttcggcta gatttagctg gaatttcaat ctctactggc tcagcctgta
     2641 ctgcaggcgt tgtccaatcc agccatgttc ttgaagccat gtatggcgca aattcagaac
     2701 gcttgaagga atcccttcgc atcagtttgt cgccacaaaa taccgttgaa gacctacaaa
     2761 ccctcgcaaa aaccttaaaa gaaattatcg gaggttagcc atatggcatt tgaaaaaatc
     2821 attcagttaa aaaattgtcg ttacgattac actcttagcc cttctgttaa aaaattcacc
     2881 ctcaaagata acaccttttt tgaaactaag gttggtaact atgaactgac tcgccttttg
     2941 gaaaaagtgc caaacagcgg tgaaggcttc caactcaaaa tcatcattaa caaggaactt
     3001 acaggggcta aaatcaatat cactgacaag tttggccttc gtctagttga tattttcaaa
     3061 tcagaagacc accatattca tcaggaaaaa ttctacttcc tcatggatag cttggtagaa
     3121 cgtggtgtct ttacaaaatc ggaaagatag agccaaaatg tttgaactga cctataagga
     3181 ctgctatcat gtagagcgta ctctcaagta tgaggatcat gaggctctca tgttgacttt
     3241 gtcaggatgt gtaaccttac cagatacact ttatgtgact tctctgactt ttaggggcca
     3301 aaaagtttat cagggtct
//
LOCUS       DAVLTM010000150         3313 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLTM010000150 DAVLTM010000000
VERSION     DAVLTM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.151"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(312..1508)
                     /gene="tuf"
                     /locus_tag="VYN16_001940"
     CDS             complement(312..1508)
                     /gene="tuf"
                     /locus_tag="VYN16_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1404421.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2419..3288)
                     /locus_tag="VYN16_001941"
     CDS             complement(2419..3288)
                     /locus_tag="VYN16_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1404422.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT         1005 a          666 c          657 g          985 t
ORIGIN      
        1 gcttcgcctt gtcgtataga tgttactgac ttcgtcagtt ctatctacaa cctaaaaaca
       61 gtgttttgag cagtctgtaa ctaactttct atagtaaaat gaaataagaa caagacaaat
      121 cgatcaggac agtcaaattg atttctaaca atgttttaga agtagatgtg tactattcta
      181 gtttcaatct attatagttt actctttgat tttcattgag tattagttca atatctaaaa
      241 aaagaacctt gccaagcaag attcttttag tgtctgactt aaataattgt tcttctggga
      301 actaaatcga attaagcttc gatttctgta accatacctg aaccaacagt acgtccaccc
      361 tcacggatag agaatgtagt accttgttct acggcgattg ggtgaatcaa ctcaacgtcg
      421 attgtcacgt tatcaccagg cattaccatt tcagtacctg ctggaagttc gattgaacct
      481 gtaacgtcag tagtacggaa gtagaattgt ggacggtagt tgttgaagaa tggagtgtga
      541 cgtccacctt cttctttagt aaggatgtag acttcacctt tgaatttagt gtgtgggttg
      601 attgaacctg gtttagcgat aacttgtcca cgttcgattt catcacgttg aacaccacga
      661 agaaggacac ctacgttatc tccagcaaga ccttcgtcaa gttgtttacg gaacatttca
      721 acaccagtaa caactgcttt ttgagtttct tctttgatac caacgatttc gatttcgtcg
      781 ttgactttaa cgataccacg gtcgatacgt cctgaagcaa ctgtaccacg tccagtgatt
      841 gagaatacgt cctcgactgg aagaagcaat ggtttgtcag tgtcacgttc tggttctggg
      901 atatactcat caactgtgtt catcaattcc ataacgatgt cttcgtattt agagtcacct
      961 tcaagagctt taagtgctga accttggata actggaagat cgtcacctgg gaagtcgtat
     1021 tctgacaata ggtcacggat ttccatttca accaattcaa gcaattcttc gtcgtcaacc
     1081 aagtcaactt tgttcatgaa gacgataagg tgtttaacac caacctgacg tgaaagaagg
     1141 atgtgctcac gagtttgtgg cattggtccg tcagttgaag ctactacaag gatagctccg
     1201 tccatttgag cagcaccagt gatcatgttt ttaacgtagt ccgcgtgtcc tggagcgtcg
     1261 atgtgagcgt agtgacgttt ttcagtttcg tactcaacgt gcgcagtgtt gatagtgata
     1321 ccgcgttcgc gttcttctgg agcagcatcg atagacgcat agtctttagg ttggttaact
     1381 gatgaaggca agcgacgtgc caaaacagtt gtgatagctg cagttagggt agttttaccg
     1441 tggtcaacgt gtccgatagt accaatgtta acgtgtggtt tactacgatc gtatttttct
     1501 tttgccattt gagtaaaagc ctccaataaa atatatttta tagatagaca gtaggcaata
     1561 cagtctaact ttccttacta ttttatcaaa tttcagctaa attgcaagtg ttttacaacg
     1621 tttttgtgaa ttattcttgg taaaaattta actaattaat agaaagtact aaatagactt
     1681 atccttcaag gagttttcag cattatccat ccactcactt gctgaaaatg taaacagtct
     1741 ctggtttata tagtaaaatg aaacaagaat agggcaaatc gatcaggaca gtcaaatcga
     1801 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatcta ctatacaacc
     1861 tcaaaactgt gttttgaaca acctgcggct agcttcttag tttgcccttt gattttcatt
     1921 gagtataaaa ttgcagataa aacgataaca atcataggac tcagcagagt caatgtaggt
     1981 cgaagaatag cccccctaat cgcttatttt atagcaaaat gaaatagaaa ctgaacattt
     2041 tgttgaggaa ggtaaatcaa ttcctcacaa tgtttgagaa agaagggggg actattccaa
     2101 cttcaatcca ctataaagca gacttacaag atagggaacc catctccgat aatatatagt
     2161 agaatgaaaa ctgcacaatt tgattggaga attcaaacca atttctaaca atgttttaga
     2221 aacaagggtc tattatttca actgcaacct actatagccc attgaagctg gaatagccca
     2281 ctgaaacttc taaaccactg ctggagagga gtttgtttag agtttctttc atttccctaa
     2341 aaaatcaaaa agagagaacc agtctgatcc tctcttactt atagatggtg ctcactcaaa
     2401 tgttaaagga gctatttctt agagataaag gaatttgaat acagctactg ccgcaattgc
     2461 tgctgcgata ggtgctacta ctggtaccca agaataccac cattttgaat cgcctttatg
     2521 ctcaccaaga actgattttg gaaggaaagc atgaaggaga cgtggtccca agtcacgggc
     2581 tgggttcaag gcaggtcctg taggtcctcc aagtgatgtt accaaagcca taacgaggaa
     2641 tccaagtgcc aagtgagcca cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt
     2701 aatagccaag tcagaaaaat caactgtttg tcctgcttct attgcctttt gtttcatgaa
     2761 ttgaagcact tcagcaccaa agaagttttt agtcaaacca agagctgcaa agaacaaaac
     2821 aaatgaacca acaaactcat taatcaaacc attgacagtt gctgcatagc gactttcttt
     2881 tgtaccatgg tcaatacttg aaatagttga gaaagttccc aagatgttat ttggtttttc
     2941 agttttcaag tagaatggac ggtatgttgc cacaactaag gcttggccaa agatagcccc
     3001 caagacttgc gcgataatgt aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc
     3061 aagccctaga gtgaaagcag ggttgatgtg attcccagat acgttaccaa acatcaaggc
     3121 tgggatcata acccccatac cataaccaac agcgatgacg atccagccac tttggtgacc
     3181 tttcgtacct ttaagttcaa cgttggcaac tgcaccattc ccaagaatga tcaaaatggc
     3241 agttcccaaa aattcagtgg catacttcag tgcccatgtg aaatccattt gctagattct
     3301 ccttaaaatt ttt
//
LOCUS       DAVLTM010000151         3174 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLTM010000151 DAVLTM010000000
VERSION     DAVLTM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.152"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(367..2868)
                     /locus_tag="VYN16_001942"
     CDS             complement(367..2868)
                     /locus_tag="VYN16_001942"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1404423.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAYLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVTTDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYIDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT          887 a          696 c          620 g          971 t
ORIGIN      
        1 aaaaatattg cttgatttat catttgaaaa actaaagaaa gtaataaaaa ctgcaaagag
       61 agtgtctaaa gtgagtatca aatttaaact ttccttgtcc caaagacaat ttttaaaata
      121 atatgcaccc actattgaca ttactaatac tataactgac actacaataa tagaaatctt
      181 aaataataaa atttcacact tcctgctatc gttcactaat ctattcattc tgacctattc
      241 taaaagtaac tctaaagttc atactctaga gttacttctt tttatttcac aacaatgtta
      301 actaatttag ttggcttcaa tagtccagtg gactattgaa ggtggcagat agagctaata
      361 aactcgttat ttaacgacga tattaacgag tttattcggt accgcaatta ctttcacgat
      421 ttccttaccg tcaatttctg ctttgacttt ttcatcagct aaagcgattt cttgtaattc
      481 ttcacgtgat agatctttag caaccatgag tttggcacga acttttcctt tgatttggac
      541 gacaatttca atttcatctt caaccaattt gctttcgtcc caagttggcc aagctacata
      601 agagattgac tcacctgttt ctgcgactgt ttgccagagt tcttctgcca agtgaggtgc
      661 aaatggtgca atcaattgaa taaagccttt ggcatagtca atataaagct tatcttcctt
      721 gttagcagca ttgacaaaga ccataagttg ggcaatagct gtgttgaatt tgagagactc
      781 aatttgctca gtaacagctt tgactgtttc gttgtaagcc ttgtcaagag caccattgtt
      841 ttccgcaagg atttctttac ttgtaatcaa acggtaaact cggtcaagga acttacggct
      901 tccttccaaa ccttcttctg accaagcaat cgaagcatcg agtggtccca taaacatttc
      961 ataaacacga agggtatcgg caccgtattg ttccaccaca tcgtctgggt taacaacgtt
     1021 cttgagcgat ttagacatct tggctggcgc ttgctccaac tcttcccctg tttctacatg
     1081 gaagaaggaa ccatcacgtt tttcaacctt gtcggttgtc acaagagcac cacggtggtc
     1141 acggtagctt gttcccaaaa tcatcccttg gttaaagagt ttttggaatg gttccttagt
     1201 cggaacaaca ccgaggtcat agaggaattt atgccagaaa cgagcataaa gcaagtgaag
     1261 tacagcatgt tccgcaccac ccacgtagat atctactggc aaccattgtt tgaggaggtc
     1321 ctcatcagcc aatttctcag tattgtgcgg gtcaatatag cgaaggtagt accagcttga
     1381 accagcccat tgtggcatgg tgttagtttc acgacgacct ttgacaccat cttcacgagt
     1441 cacttcaagc cagtctgtca agttagctag tggactttca ccggtacctg aaggacggat
     1501 gtccttggtg acaggcaaga caagcggcaa ttcagtttca ggaacagctg ttgaagttcc
     1561 atcttcccaa tgaataattg gaattggttc accccagtaa cgttgacggc taaagagcca
     1621 gtcgcggaga cggtaggtaa ccttctcctg accacagcct ttttcttcca agtaagccac
     1681 aatcttggca atagcgtctt ctttgttcaa tccatctagg aagtctgaat tgacatgcag
     1741 gccatcctct gtgtaggcag cttcttcgac atttccacct tcaagtactt cgacgattgg
     1801 aaggtcaaat tgtttggcaa attcccagtc acgttggtcg tgggcaggca cagccataac
     1861 cgcacctgtt ccataactag caagaacata gtccgcaatc cagattggca tttccttacc
     1921 attgacaggg ttgatggcat aagcaccagt ccaaacacca gttttttcct tagcaaggtc
     1981 tgtacgaacc aagtcagact taaggctggc ttggtgttta tagtctgcta cagcttctgc
     2041 ttgctctgaa cttgtgatag cgtctactaa ttcatgttca ggagccaaga cagtgaaagt
     2101 cgcaccgaaa agtgtgtccg gacgagtagt aaagactgta aattccttgt ctgttccttt
     2161 tactttgaaa gttacattgg caccagttga tttaccaatc cagttgcgtt gcatatcctt
     2221 gatagactct gaccaatcta gttcatctaa gtcattgagc aagcgctctg cgtaagccgt
     2281 gattttgagc atccattggc gcattggttt gcggacaact ggatagcctc cacgctcaga
     2341 agttccgtca ggaagcactt cttcattggc aatggcagtt cccaattcct caacccagtt
     2401 tactggcact tcagcttcat aggccaagcc tttttcgtaa agcttggtga aaatccattg
     2461 agtccacttg tagtagtttg gatctgttgt gttgacttca cgatcccagt cataagaaaa
     2521 tccaagcgca ttgatctgac gtttgaagtt ggcaatgttt tccgctgtaa attctgctgg
     2581 gtcattacca gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat cccaacccat
     2641 tgggtgaagg acattgtagc cttgcgcacg tttgtaacga ctgaggatat cggttgcagt
     2701 ataaccttct gggtgtccta cgtgcagacc agctccagac ggataaggga acatatcaag
     2761 cgcataaaac ttaggttttg atgcatctgt tcctgtctta aatgtatgat gttctgccca
     2821 gtagccctgc cacttaggct caatttcttt atgattataa aaactcatgg tctctctcca
     2881 attttgtgat gttactatta taccattttt gagggaattt gaaatgtgtt taaagaaaaa
     2941 actgtatact gtatctcatt aattttgatg actatgaatt gctctatctc ccacttttta
     3001 tcccattgtt atgcagtaaa tcaaggagta agtcaagaag cttatctcgt atagcacaaa
     3061 atacttattt tagaccgaag ataaaactgg aatgctaatt aatgtattca caataaatag
     3121 ccgactacct atgtcgcttt ttgatttatg aagaacgtat agtagactga atct
//
LOCUS       DAVLTM010000152         3166 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLTM010000152 DAVLTM010000000
VERSION     DAVLTM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.153"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(64..333)
                     /locus_tag="VYN16_001943"
                     /pseudo
     CDS             complement(64..333)
                     /locus_tag="VYN16_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            525..2291
                     /gene="patB"
                     /locus_tag="VYN16_001944"
     CDS             525..2291
                     /gene="patB"
                     /locus_tag="VYN16_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1404424.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMGLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2430..3119
                     /locus_tag="VYN16_001945"
     CDS             2430..3119
                     /locus_tag="VYN16_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1404425.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
BASE COUNT          917 a          617 c          702 g          930 t
ORIGIN      
        1 cttaagatag gccttaaaag caggtgaaaa gcgagggcat gctttggcag cttgtatgag
       61 tacctaccgc agatgagggg aaccccgttt gaccattctt cctgctaaat caatctgacc
      121 tgactgataa atagaagaat ccagtccagc gaaagcttgt aattgagcag gattatcaaa
      181 ggcatgaata tttcgaatct cggctaaaat gaccgcccct aaacgattct caatcccagt
      241 aaccgtcgtg atgaccgagt tgaactcagc catcaagtca ttgacacatt ttttcgcctt
      301 gtcaatgagc ctcttgtaat gtttgatgat ttcattacac gagataaaac gtctatgcgt
      361 tatcaaactc attaccaatt aaaacaaaaa gctgtggtta gatcctttcg gaaatcgtca
      421 agcgattgga ggaaatgaac taatccacag cggcttattc caagtatacc acttgggctt
      481 tggcagtagc taactgcgct aaatataata taaggaggag taaaatgaag acagttcaat
      541 ttttttggca ttattttaag gtctacaagt tctcatttgt agttgtcatc ctgatgattg
      601 ttctggcgac ttttgcccaa gccctctttc cagtcttttc tggacaagcg gtgacgcagc
      661 tagccaattt agttcaagct tatcaaaatg gcaatccaga acttgtatgg caaagcctat
      721 caggaatcat ggtcaatctt ggcctgctgg ttttggttct atttatctct agtgtaatat
      781 acatgtgtct catgacgcac gtgattgcag aatcgaccaa cgagatgcgc aaaggcctct
      841 ttggtaagct tgctcagttg acggtttctt tctttgaccg tcgacaagat ggcgatatcc
      901 tgtctcattt taccagtgat ttggataata tcctccaagc ctttaacgaa agcttgattc
      961 aggtcatgag caatattgtt ttatacattg gtctgattct tgtcatgttt tcgagaaatg
     1021 tgacgctggc tctcatcacc attgccagca ccccattggc tttccttatg ctgattttca
     1081 tcgtgaaaat ggcacgcaaa tacaccaacc tccagcagaa agaggtaggg aagctcaacg
     1141 cctatatgga tgagagcatc tcaggccaaa aagccgtgat tgtgcaagga attcaagagg
     1201 atatgatggc aggatttctt gaacaaaatg agcgcgtgcg caaggcaacc tttaaaggaa
     1261 gaatgttctc aggaattctt ttccctgtca tgaatgggat gggcctgatt aatacagcca
     1321 tcgtcatctt tgctggttcg gctgtacttt tgaatgataa gtctattgaa acaagtacag
     1381 ccctaggttt gattgttatg tttgcacaat tttcacagca gtactaccag cctattatcc
     1441 aagttgcagc gagttgggga agccttcagt tggcctttac tggagctgaa cgaattcagg
     1501 aaatgtttga tgcagaggag gaaatccgac ctgaaaaggc tccaaccttc actaagttgc
     1561 aagaaagtgt tgaaatcagt catatcgatt tttcatactt gcctgataaa cctattttga
     1621 aagatgtcag catttctgcc cctaaaggcc agatgacagc agttgttggg ccgacaggtt
     1681 caggaaaaac gactattatg aacctcatca atcgctttta tgatgttgat gctggtggta
     1741 tttattttga tggtaaagac attcgtggct atgacttaga tagtcttaga agcaaggtgg
     1801 gaattgtatt gcaagattcg gtcttgttta gcggaacgat tcgagacaat atccgatttg
     1861 gtgtgccaga tgctagtcag gaaatggttg aggtagcagc aaaagcaacc cacattcacg
     1921 actatatcga aagtttgcct gataagtacg atactcttat tgatgatgac cagagcatct
     1981 tttcaacagg gcagaagcaa ttgatttcaa tcgctcgaac cctgatgaca gatccagaag
     2041 ttctcattct cgatgaagca acttcaaacg tagatacggt gacagaaagc aagattcagc
     2101 atgccatgga ggtggttgta gcaggtagaa ctagtttcgt cattgcccac cgcttgaaaa
     2161 ccattctcaa tgcagatcag attattgtcc ttaaagatgg agaagtcatt gaacgtggta
     2221 accaccatga acttttgaag ctaggtggct tttattcaga actctatcac aatcaatttg
     2281 ttttcgaata agaaagaagt tgtcctatgt gggcagcttt ttcttgtcca taaaaaatgt
     2341 ttatcacagc cttaaaaaaa acatattaga cgaaagtcat tttgagtgat atgataggac
     2401 tatcgttagc attcgaaagg agaggcatca tggctagaac ggttgtagga gttgctgcaa
     2461 atctatgtcc cgtagacgca gaaggcaaaa tcattcattc atctgtatct tgtagattcg
     2521 cagagatcat tcgtcaagtc ggtggtctcc ctttagtcat tcctgttggt gatgagtcag
     2581 ttgtacgtga ttatgtggaa atgattgaca aactcatttt gacaggaggc caaaatgttc
     2641 atcctcagtt ttatggagag aaaaagaccg tcgaaagcga tgattacaat ctggtccgtg
     2701 acgaatttga attggcactc ttgaaggaag cgcttcgtca gaataaacca attatggcaa
     2761 tctgtcgcgg tgtccaactt gtcaatgttg cctttggtgg aaccctcaat caagaaatcg
     2821 aaggtcactg gcaaggacta cctttcggga catctcactc tattgagaca gtagaaggaa
     2881 gcgtggtggc taagctattt gggaaagaaa gtcaggtcaa ctcagtccat cgtcaaagca
     2941 ttaaagattt ggcacctaat ttccgtgtaa ctgccattga ttcaagagac caaactattg
     3001 aagcgattga gtctatcgac gagcaccgca ttatcggttt acagtggcat ccagagtttc
     3061 tggttaatga agaagatggc aatttagaat tatttgagta tttattgaat gaactgtaac
     3121 gattggaaca tctggtcgtt ctttctttta ttttttcaaa attttt
//
LOCUS       DAVLTM010000153         3052 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLTM010000153 DAVLTM010000000
VERSION     DAVLTM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.154"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..1811)
                     /locus_tag="VYN16_001946"
     CDS             complement(<1..1811)
                     /locus_tag="VYN16_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1404426.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGI"
     gene            complement(1798..2163)
                     /locus_tag="VYN16_001947"
     CDS             complement(1798..2163)
                     /locus_tag="VYN16_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1404427.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            complement(2168..2524)
                     /locus_tag="VYN16_001948"
     CDS             complement(2168..2524)
                     /locus_tag="VYN16_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404428.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
BASE COUNT          868 a          636 c          503 g         1045 t
ORIGIN      
        1 tctatcccat ctccttttga aagtcctgta tttaagcgat ttataaattt atctagcctg
       61 actaccatct tttcatacct gcccctttcc aattccagtt gattccccag gctcttcaat
      121 tttttctcaa caatattagc actgatattc tcagttatat tcagttttga aagatttaat
      181 ttcatttctt gcttactttc aactgaagcc agtagatatt ctgctacttg attcaaggtt
      241 gacattcgtt cttgcaactc attgattctc aattcagacg ctgctagttc atgcactaac
      301 tcatctttaa tcgtgacata agattgattt tctacttcca gttcaatcaa agcatccact
      361 tccgcaatct tctctttaat catatcgact gtcgcttttc tgcgtaatgg aatggtttga
      421 ttttcatagc tgaactgtct aattaaggtt cgacctttca tataacaatt tttctcggca
      481 gcgtctttgt ggtagacata gtaggagctg gtttccctga tgaaaacctt caccttatcc
      541 tcttccatat caagctgcat gttcggcaca aagataagtc cctcctgacg aataccaaac
      601 ttgaccttga cgtaaattcc atcatccact actttttcaa tttgattgag tgacaactcc
      661 acctcaaatt catgaacggc atctctgtta ctcttgaact cttgatagga ctcccagaac
      721 ttctcatcgc ttggaagttc tttttctttg gataactttt cttcttgata aagttgaacg
      781 aaatcctcag tttctggagc ttgccaatct tttctatttt taaaataatc ttggaaaaac
      841 tctacatcat aaagattttt ctggtctagc tctgtctcct ttacctccac tcctgcaaat
      901 tgaaaagaaa catgcttttg tttaggattg atagttagtc caaaaagttt tgctttctga
      961 agtaaatcat ccatattctc aactttcagc aataaaaatt ccatgagact ttctatttct
     1021 cttttggcaa agtagttctt aaaaaattct tctgtgtaag gctgcttgcg attgagttgc
     1081 ttgccacgca ccacctgttt catagttgag tccgtaataa aaaaggtggc atgcttgtga
     1141 cggaaatcca tctccacatg tagtagcgga gcattctttt tgaaatcctc aaagtcccta
     1201 gaatgttcca tcaaaaaata gagtcgctgc ttgagttcat acttgtgatt agtcttacga
     1261 tagacttcat accgctggtg agaatagcgg ttctcaatga ttttagcacc tgcgatttta
     1321 gaaaaacggt cagaaatcat gcgaagattt cgctccacct tgtagtccca cttgagcttt
     1381 ttgtcagaat tgctatctac tgaattgata atgatgtgat tgtgcaggtg gtctttatca
     1441 acatgggtcg caacgataaa acgaaatttg ccaccagtta attccttcac agtctcataa
     1501 cctatccgat tgatttgttc aggagtgaga tgatcctctg gcgagaaaga ctgaatgatg
     1561 tggtgagcgt gtatttttcg ttgattttct tccatcctgt cgtggcgaaa atcgtaaagc
     1621 gtatcgttgc tgatgaaatt ttcatgatac atctgcacca tttcctcata gctaggaaag
     1681 tccagaaaat tcttcatgcc atagtccgac accaaggcaa gattattggt tttctcagga
     1741 ttgagaatgt acttgataag ctttctgcgg taactctttc cgtgaatggc aaagtgttta
     1801 gtgatgacca tggaactcct ttagcttctg cgcttgcaga ttaaattcct tatcaacttc
     1861 ttttatcaat tcaccaattc cttttttcaa ctcctgcaat tcttcaccag aaattaactg
     1921 agattgatta acacttcgag caatctggtt gatattattg ccaatcctct ttaactcaaa
     1981 cactaaatct tggtaaccgt ttgtgtcaat cgtgataaaa ttcataccag gatcgagtag
     2041 agttcgtctc gcatattcag aaaaggattt acaaccactt tgatcgatat ttatatttag
     2101 ttggttcaac tcactatcag acaaaaacac tttcttgaga ttggttcggt aacgatgttc
     2161 catgtgctta cctcatgtac ttctcacgaa attctttact gaggggtatg gtgtttgcaa
     2221 cctcttgaat caattcctgc acgcacgtta agagaataga tacatgctct tgggtgactt
     2281 gacgttctga ctgtgccaag attaaaactt catgaacgtc acgactgatt tgttctagtt
     2341 tttgggattg ccagagggca aaccatgtct ccatctgttt ttgtaaatca gaggaaagta
     2401 aacttttacg aagaaaatca gagaaattag tctctccccg ctctctcatc aaagctagaa
     2461 tttgcttttc ttctgcttct gttaaacgaa attgtttccg aatatcacgc acatctcgtt
     2521 tcatactgct acttcctttc caaaatactt gtactgacaa tgtgagctac catacaatgt
     2581 cagtacactt ttccaacaac tgattttcat tcttgtaagg ctttgcgagc tcagatattg
     2641 tgtccacaat atcccaaaaa tcatatcgcc agcctatcaa aactttctag ttttgacagc
     2701 caacgataag ataacttggt gacttcgccc ccaaaccccc atagaaaatc aaaaattgat
     2761 tttctatgaa tgatattagg ataacaggga agatttgaaa aaggtatcac agctaatata
     2821 gttatgattg actttctctg attctatgat aaaatttctg taaactaata tttggagaaa
     2881 taaaaatgct gagtctagat caaatacact tactattgaa tacacccgaa gatgagtttc
     2941 atgattttaa acaaaaatgg catcattcaa aaactgaatt ggtgcgtgat atcttaaatt
     3001 ttgtcaatac atcacatcac gaagattgtt atatcatctt tggaattgat aa
//
LOCUS       DAVLTM010000154         2989 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLTM010000154 DAVLTM010000000
VERSION     DAVLTM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.155"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(130..1071)
                     /locus_tag="VYN16_001949"
     CDS             complement(130..1071)
                     /locus_tag="VYN16_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1404429.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1049..2812)
                     /gene="aspS"
                     /locus_tag="VYN16_001950"
     CDS             complement(1049..2812)
                     /gene="aspS"
                     /locus_tag="VYN16_001950"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1404430.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          875 a          700 c          601 g          813 t
ORIGIN      
        1 agatgaaact gacgaagtca gctcaaaaca tggttttgag gttgcagata gaactgacga
       61 agtcagtaac atatatacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaagac
      121 tattaatcac taattatcta tctcaacaaa tcttcctaga atatgaacat tttccgagac
      181 agagacaaag gagcttggat ccacttgtgt cataatctgt ttaaattcat taaactctgc
      241 acgtgtaatg acagtgatta aaactgcctt tctctcgtga ttataggttc cttctgcatc
      301 gtggatcatg gttgctccgc ggtgcaattt tttatggatt ttttcaatta ccttctctgg
      361 atgatttgtc acaatcatgg cctgcatacg cttttgctta gtaaagactg cgtctgtcac
      421 acggctagag acaaagatgg taatcataga ataaagagcg tatttccaac caaaggtcaa
      481 acctgctatc agcatgatag ttccatttac caagaaagaa atactaccga cattcttacc
      541 cgttttctta cgaatagtca ggctaacgat atccgtccca ccactggaga tattgtttcg
      601 aagagcaaaa ccaatcccca aacccataac aacaccccca aaaagggaat tgataatggg
      661 atcctccgtc aaggttgcca cagggacaaa ctggataaag aaggaactca tagataccgt
      721 gataaaggta aagacggtga acttatggcc aatctgatac caagctaaga ccatcaaagg
      781 gaagttaatg gcgtagaagc ttagcgaaat cggaatatga aaaccaaacc agtgattact
      841 caaggcagag ataatctgtg ccagacctgt tgcaccactc gaatacacat gccctggttg
      901 gaaaaagaaa ttaactgcta ctgctgataa aaaaccatag accagagagg ccgaaatctt
      961 ctcatcatac ttttctcgag agatactttg taagacacgt aaaattttta tctgataagc
     1021 aaagcggcgc agataatagc gccaccgctt aattcgtttt gtttgtttca tcttcttcta
     1081 cttgtaagct gagttcctct agttgtttga gagcgactgt tgatggagct tgtgtcattg
     1141 ggtcagttgc cttgttgttc ttaggaaagg caatgacttc acggatattt tcttctccag
     1201 caagcaacat gacaaaacgg tcaagcccga tagccaaacc accgtgtggt gggaaaccat
     1261 agtccatggc ttcaagaagg aaaccaaact ggtcattggc ttcttcagct gagaaaccaa
     1321 gagccttgaa catgcgttct tgaaggtctt tttggttgat acgaaggcta ccaccaccaa
     1381 gctcataacc gttcaagacg atatcgtaag caatggcacg aaccttagcc aaatcacctt
     1441 ctaattcgtg agcagtctct tcctgtggaa gggtgaaagg atggtgggcg ctcatgtagc
     1501 ggccttcttc ttcagaccat tcaaacatcg gccagtcaac cacccaaagg aagttgaact
     1561 tatcattatc aatcaagcca agctctttag caatacgtcc acgaagggca cccagtgttg
     1621 cattagccac ttcaagcgta tccgccacaa agagaaccaa gtccttatct tcaagagcaa
     1681 gcgctgttgt caattcttct tggataccag tcaagaactt ggcaactggt ccgtttaatt
     1741 ctccatcaac caccttaacc caagcaagac ctttggcacc atactgtttg gctacttccg
     1801 tcatcttgtc gatgtcttta cgtgaatagt tgtccgcagc tcctttgacc acaatcgctt
     1861 ttacagcagg tgcttctgaa aagactttaa agtctacacc tttgaccact tctgtcaagt
     1921 cctgaagcaa catgtcaaaa cgagtatctg gcttgtcaga accgtaaaga gccatagcat
     1981 catcgtattt catacgaggg aacggtagcg ttacttcgat gccttttgtt tccttcatca
     2041 cgcgcgcgat caagccttct gtaatatctt ggatttcttg ctcagtaagg aaggacgttt
     2101 ccaagtcgac ctgagtaaat tcaggctggc ggtctccacg caagtcctcg tcacggaaac
     2161 atttaacgat ttggtagtaa cggtcaaaac cagcattcat caagagctgt ttcgtgattt
     2221 gtggactttg aggaagagcg taaaaatgcc ccttattaac acgagacggt actaaataat
     2281 cacgcgcccc ttcaggcgtt gacttagaaa ggaatggtgt ctccacgtcg ataaactcca
     2341 actcatccaa atagttgcgg atagagtggg tcaccttggc acgaagttta agattttcca
     2401 acatttctgg acgacgaagg tcaaggtaac ggtaacgcaa acgtgtatcg tcatttgcct
     2461 caatgccatc cttaatctca aatggtgttg tcttagctgt gttaagcaca ataagagctg
     2521 tcacgtttaa ctcaaccgca ccagttggca acttatcatt ggcttgctca cgcgcagcga
     2581 cctgaccagt cacctcaata acaaattcgc tacgaaggct ttcagctgtt gccataacct
     2641 ctgcagatac tttttcaggg ttgataacca actgcatgat tccttcacgg tcacgaagat
     2701 cgataaagat caaaccacca aggtcacgac gacggccaac ccatcctttc aaggttattt
     2761 cttgtccgat gtgttcctca cgaacacgac cagcatacat actacgtttc attatttctc
     2821 tcctctttta ttctgttact attttaccat aaaagcgcag gctcttcatg aaaatcatca
     2881 gaaaagtttg ccagtcttta aaagtcaggt gaaagcccta aaaattagcg ctaatactct
     2941 tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt agtacggtt
//
LOCUS       DAVLTM010000155         2934 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLTM010000155 DAVLTM010000000
VERSION     DAVLTM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.156"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(1..315)
                     /locus_tag="VYN16_001951"
                     /pseudo
     CDS             complement(1..315)
                     /locus_tag="VYN16_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            354..1271
                     /locus_tag="VYN16_001952"
                     /pseudo
     CDS             354..1271
                     /locus_tag="VYN16_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1311..2255)
                     /gene="manA"
                     /locus_tag="VYN16_001953"
     CDS             complement(1311..2255)
                     /gene="manA"
                     /locus_tag="VYN16_001953"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1404431.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEDELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADNLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2387..2668)
                     /locus_tag="VYN16_001954"
     CDS             complement(2387..2668)
                     /locus_tag="VYN16_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1404432.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYMSFIAMV"
     gene            2733..2855
                     /locus_tag="VYN16_001955"
                     /pseudo
     CDS             2733..2855
                     /locus_tag="VYN16_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          798 a          675 c          536 g          925 t
ORIGIN      
        1 agaggagttc ttgaaatcgt aacaccacct ttgaacaaga gttacttcaa cccattggct
       61 ccgacggagt aagttgcttt cgtggatacc aaaatcagcc gcaatttctt cataagttcg
      121 atattctcgc acatattgaa gagtggccat aagaaggtct tctaggctta atttaggttt
      181 tcgtccacct tttgcgtgtt taagttgata agctgttttt aatacagcta acatctcttt
      241 aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
      301 tgcttcatca ttcatagaac tactatgcca tattttgttt cgcaggaagt ctgatcattt
      361 caaatccagc cattgctcta ggagctcaag cgacctttat cctattgaat atcttcattg
      421 tatcacgtgg ggttcaaaaa gggattgaaa gagcttcgaa agtcatgatg cccctgctct
      481 ttatcatctt tgtcgtcatc atcgaacgct ctctcagttt gccaaatgcc atggaagggg
      541 ttctttactt cctcaaacca gatttttcaa aactgactag cgctggtctc ctctatgctc
      601 tgagacaatc tttctttgcc ctctcactag gggttacagc catgttgacc tatgcttctt
      661 acttggacaa gaaaaccaat ctggtccagt caagaatctc catcgtagcc atgaatatct
      721 cggtatccat catggcaggt ctagccattt tccaagctcg gtcccccttc aatatccagt
      781 ctgaaggggg accgagcttg gtctttatcg tcttgcctca actctttgac aagatgcctt
      841 ttggaaccat tttctatgtc ctcttcctct ttgcgactgt cacttcttct gtcgttatgc
      901 tggaaatcaa tgtagacaat atcaccaatc aggacaacag caagcgtgct aaatggagtg
      961 ttattttagg aattttgacc tttgtctttg gcattccttc agccctatct tacggtgtca
     1021 tggcggatgt tcacattttt ggtaaagcct tctttgacgc tatggacttc ttggtttcca
     1081 atctcctcat gccatttgga gctctcttcc tttcactttt tacaggctat atctttaaaa
     1141 aggctcttgc aatggaggaa ctccatctcg atgaaagagc atggaaacaa ggactgttcc
     1201 aagtctggct cttccttctt cgtttcgtca ttccaatcat catcgtggtc ttcatcgccc
     1261 aatttatgta atcaaaagga cttgagtaat gaactcaggt cctttctttt ttatggatga
     1321 ctaacaatta attctaaatc ttgcccttct agagtccaag attcaacatc acttggtagg
     1381 ataaagtgtc tccctttttg gattggatag tttttcccgt caacagttag ttgaccttga
     1441 ccagccaaga cactcaacaa gctgtagtca gctgtcttct caaaatcaac ttttccagtg
     1501 atttcccact tgtaaactgc gaagaaatca ttagaaacaa gtaaagtgga acgcaaatta
     1561 tctgctttaa cagttacagg acggctattt gctggctcgc caatgttcaa aacatcgatg
     1621 gatttttcaa ggtgaagttc acgcaagttg cccttatcgt cctttcggtc aaagtcatag
     1681 acgcggtaag ttgtatcact agactgttgg gtttcaagaa tcaagatgcc agtaccgata
     1741 gcatgcatgg tgccacttgg tacatagaag aaatctccag ccttaacagg gactttggtc
     1801 aacaagtcat cccagttctt gtcctcgatt tgctggcgga gttcttcttt tgacttggca
     1861 ttgtgaccgt agataatctc tgaaccttca tctgctgcaa tgatgtacca acattctgtt
     1921 tttccaagtt cgtcttcatg ctcgagtcca taagcatcgt ctgggtgaac ttggacactg
     1981 agccagtcgt tggcatcgag gatcttggtc aaaagtggaa atacaggttc tggacgattg
     2041 ccaaataatt cacggtgttc cgcatacaaa gtagcaagat ctgttccctc gtaacgacca
     2101 ttggcaactt tagaaacacc atttagatga gctgagatag cccagtactc tccgactttt
     2161 tcacttggga tgtcgtagcc aaactcatca cgtagcttgg taccacccca gattttttct
     2221 tgcataactg attgtaaaaa taatggttct gacatcttaa tctcctgtct gatttttctc
     2281 ccctcattat agcaaaaaaa gagttccatt tgaactcttt tttacatctt ataaagcagg
     2341 gagaagattt tataaaaata gtaaacaaat gtgctctact cgatgcttac accattgcta
     2401 taaatgacat gtaccaatag aaggacttct tcttgctacg tttgagagct ccgtttccta
     2461 cattatctcg atctacatag ataaagccat agcgcttatt catttcccct gtgccagctg
     2521 aaaccggatc gatacagccc catgtcgtat aaccaagcaa gtcaaccccg tctttgtaaa
     2581 tggcatctcg catggccttg atgtgggcct ctaagtaagt aatccgatag tcatctgcta
     2641 cataaccatt ctcatccggt gtatccatag caccgagtcc attttctacg ataatactat
     2701 actaaaatca aaaagcatta tataatagtg atatgaaatc aactaaagaa gaaatccaaa
     2761 ccatcaaaac actttttaaa gactctcgta cagctaaata tcataaatgc cttcaaatca
     2821 ttctatttcg tctgatgggc aaatcttata aagattccga ctaatattgg ttttaccttt
     2881 attcctccat acacaccaga gatgaaccca tcgaacaagt gtggaaagag attc
//
LOCUS       DAVLTM010000156         2934 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLTM010000156 DAVLTM010000000
VERSION     DAVLTM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.157"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(136..1059)
                     /locus_tag="VYN16_001956"
     CDS             complement(136..1059)
                     /locus_tag="VYN16_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1404433.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(1073..2002)
                     /locus_tag="VYN16_001957"
     CDS             complement(1073..2002)
                     /locus_tag="VYN16_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1404434.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(2321..2542)
                     /locus_tag="VYN16_001958"
     CDS             complement(2321..2542)
                     /locus_tag="VYN16_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404435.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTEAELIDNLKEVIAKLKANA"
     gene            complement(2496..2756)
                     /locus_tag="VYN16_001959"
     CDS             complement(2496..2756)
                     /locus_tag="VYN16_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404436.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT          934 a          581 c          577 g          842 t
ORIGIN      
        1 tagataagac tgacgaagtc agttacatat atctacggca aggcgacgtt gacgcggttt
       61 gaatttgatt ttcaaagagt attaacttca cacaagggaa gttgggaact gagaaatgtt
      121 ctttttcaat aagcactatt ctttcacacc accgatagtc aaacctttta caaagtagcg
      181 ttggaagaat ggatataaaa tcgcgattgg aagggtcgca accacaacca tggccatacg
      241 acctgtttct ttcggtagag caactcccag ttgaccagtc aagccgaccg ctttggcaat
      301 gtagtccata ttttgttgga tttgcatgag caaatattgc aatggataca agttgtcact
      361 cttgatgtaa agaagggcgt tgaaccagtc attccagaaa ccaagagctg tcaaaagcgt
      421 gatggttgcg atacctggta gtgacaatgg caaacagatt tggaagaaaa tccgggcctc
      481 actggcacca tcgatacgag ccgattcgag aatggcttct ggaatggtct tcttgaagaa
      541 ggaacgcatc aagatgatat tgaatggtga gagaagcatt ggaacaatca aggcccaaac
      601 agtgtcacca agctgaagta cacgggtcac catgatataa cctggtacca aaccagcgtt
      661 gaacaacata ctgagaagga cgaagatggt aaaaaatctg cgatacttaa aggttgtccg
      721 tgaaatagcg taggcatagg ttgttgtgat aaagacattt gtcaatgtcc caactacggt
      781 tacaaagaca gagatgaaga gggcttgtag gattttatcc ttaaactgtg ccaaaaactc
      841 aaaaccgtct aagccaaatt gggatgggaa gaagctatag ccgtattgga ggaggctttt
      901 ctcgtctgtc actgaaataa tgataacgaa tacaaaaggt aggatacaag agagggcaat
      961 caaacccgaa atgatactga agaagatatc tgctttctta ctgaaggagt gaatgccgac
     1021 attatcaatt ttttcttttt taattttctt ttttgccata ttctcctcct ttctagaaca
     1081 aagctgagtt tggatcgact cgtcttgcaa gcaagtttga taggataacc agaatcaaac
     1141 caacaacgga ttggtaaaga ccggctgctg cagccatacc gatatctgct gtctgagtca
     1201 aaccattaaa aacatatacg tccaaaacgt tggttacatt gtaaagctga ccagcattgt
     1261 gtgggatttg atagaagaga ccgaagtctg cgcggaagat atttccgact gcaaggatgg
     1321 tcaatacagt tacaagcgga gtcaactgag gaatggttac gttgcgaata cgttgccact
     1381 tgctagctcc gtccactgtc gctgcttcgt agtaggttgg atcaattccc atgatcgtcg
     1441 catagtacat gacactgcta tatccaaaac ctttccaaat acctaggaaa agtaggagat
     1501 agggccagat gcccaggtca gcgtagaaat tgacttcttt gagaccaaga ctttccaata
     1561 gatgattgaa caccccttta tcaatattta ggaaggcatc tgtaaagaaa ctgatgataa
     1621 cccaagacaa gaagtaaggg aacaacatag aagtttggaa aatcttcacc attctcttag
     1681 aacggagctc gctgaggata atggcaatcc ctacagatac aactaaacct agaaagataa
     1741 agccaagatt gtagaggaca gtatttcgtg tgataataaa ggcgtctctt gaactaaata
     1801 agaatctgaa attatcgagt ccgacccatt tactatttat gatactatct atgaaaccat
     1861 tactggtcat gtggtagtct ttgaaggcaa ccacgttccc aaatactgga atgtaaaaga
     1921 atagaatcaa ccagagtgcc cctggcaaaa ccatcaagag aaagatccag ttgtctctca
     1981 atgtttttga aaactttttc ataatttcct ccctttttat tttgatatcc atctaaaaat
     2041 tcttttttag acttttgata acgattacat tattagtata ctcctatttg caggttaggt
     2101 taaactccta attatagaaa aaactccaca aattatgtag cagatttaaa actttatcac
     2161 cactataaaa caaatgtcct aaatcaattg tttattttat ctctattagc ccagtgatgg
     2221 cgtcactctg ttataagcat ccaacaacgg ggtatactga aaaatctcca gactagggaa
     2281 ctcagcgata gttcctaatc tggagatttt taatatgtta ttaggcgttt gctttcaact
     2341 tagcaataac ctctttaaga ttatcaatca actctgcttc agtatgctca gagccttttt
     2401 catctgccaa gaacaaaact gctttttgaa gttcttcttg agagttttca aggacatcct
     2461 tatctactgt ttcaaggttt gagtctttaa gaagtttact taattccttg gctaatttct
     2521 tgagtttgat ttgcagactc atcttctcct gctgtttctt tgcccgctgt ttgtcctcca
     2581 tccttagttg ctgactggct ttccttaatg gactctaggg aagcaatggc atctttgact
     2641 gtttgcaaga tatcacgtaa accttgctct gtcaaactat catctgcaag agctttctta
     2701 gcctctgcca aaaccagacg tgctgaatct gtggtaggat tcgatacacc tgtcaatgat
     2761 ctcaaaagat tttctaaggt ttgagtctgc ttactaatac tagactaaaa tcaaaaagca
     2821 ttacataaca gtgatatgaa atcaactaaa gaagaaatcc aaaccatcaa aacactttta
     2881 aaagactctc gtacagctaa atatcataaa cgccttcaaa tcgttctatt tcgt
//
LOCUS       DAVLTM010000157         2919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLTM010000157 DAVLTM010000000
VERSION     DAVLTM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.158"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(75..455)
                     /locus_tag="VYN16_001960"
     CDS             complement(75..455)
                     /locus_tag="VYN16_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1404437.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(877..>2919)
                     /gene="phtB"
                     /locus_tag="VYN16_001961"
     CDS             complement(877..>2919)
                     /gene="phtB"
                     /locus_tag="VYN16_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1404438.1"
                     /translation="IRTKEEIKRQKQEHSHNHGGGSNDQAVVAARAQGRYTTDDGYIF
                     NASDIIEDTGDAYIVPHGNHFHYIPKSDLSASELAAAQAYWNGKQGSRPSSSSSHNAN
                     PAQPRLSENHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLIFDPAQITSR
                     TARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEP
                     SPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGI
                     DSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDN
                     LLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNSQITYTDDEIQVAKLAGKYTTE
                     DGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH
                     QDAGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFE
                     WFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQ
                     TEKPSEEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQVETEKVEEK
                     LREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          770 a          602 c          564 g          983 t
ORIGIN      
        1 gaaatcattt acttaacatt ttatagttga gataatcaat agcttaccta taaaaagagt
       61 tatagtaaaa ttccttattt attgatttca agctccgcta actgtatttg aataactgac
      121 agttctgcac cagcctgaag aagagcagct gcattatagg caccttctac aattggaacc
      181 ctgttgatga tgatactttt atcactgaaa tcagtcacca tttttaagtt cattttagca
      241 gaacctaggt caaaaaatac aagtaaagta tctgctggat tttcggaaac aaccctatct
      301 acttgatcaa aactcgttcc aattcctccg ccctcggttc ctcctacata agtaatcgga
      361 acatctttag ctactttact aatcagttca acaacacctt ctgcaatgtg tttggaatgt
      421 gaaacgataa caagaccaat accaatactt tccatcaaac cactccagtt tctaaaatag
      481 cagtaaagag taatcctgat gagaatgatc caggatcaat atgtccaaga aaccacatgc
      541 tcctaagaca agagctaaca gactggccgt caataatagt attgttcttt ttttcatcat
      601 tactccttaa ctagtgttta actgattaat tagccagtaa atagtttatc tttatttaca
      661 ctatctgtta agatatagta aaatgaaata agaacaggac agtcaaatcg atttctaaca
      721 atgttttaga agtagaggta tactattcta gtttcaatct actatatttt gcacattttc
      781 atgaaaaaaa tgagaactag aactcacatt ctgctctcat ttttcgtttt cccgttctcc
      841 tatcctgttt ttaggagtta gaaaatgctg ctacctttac ttactctcct ttaataaagc
      901 caatagtttt tcagcttctg ccataatagt attgttgtcc tgggtgccaa atagtaaatt
      961 attttttaat cctgtgagag tctctttggc attggacttg ataattggat cctggatttt
     1021 tccaagtaaa tcttcagcct ctctcagttt ttcttcaacc ttttcagtct cgacctgagg
     1081 ttcttctgat tcctctggtg attcttctgg ttcctctgtt ggttttggag actctggttt
     1141 ctcgctttgc ggtttctctt ctcgaggggt ttcttcctca ggtttttctg tctgaggttt
     1201 ctcctcgctt ggtttttccg tttgattggt atcagcttga ccatttttgt ttctttgaac
     1261 atggtcgcta gcgttaccaa aaccattatc tgaatgcgga cgttcgtttg gatgttcgac
     1321 atagtacttg acagtcgcca aaagatcctc aagagtatac cccttaggtg cctcataaag
     1381 gccttcgtca aaccactcaa atttgatgtt atggtaatgg tcataatgag gtatgattaa
     1441 actaccgttt ttgacttcta cagtatattg aagattgtaa ggcatgcgat caagtggcac
     1501 cttcttagct gctttcacgc ggttgtagat agcttctgct ccttttgcct cagtatttcc
     1561 tgcatcctga tggtctgtcg aaggaggggt caaacctttc tctttagcat aagcctgggc
     1621 tgccgctctc tcagcttcag acaaactatc ttttttaatc cagtggctat gggtcatatg
     1681 tggagttaca taggcatccc cctcatcact ggttatatca cgaggatcaa agatataacc
     1741 gtcttctgtt gtgtacttgc ctgccaactt ggctacttga atctcatcat cagtgtaggt
     1801 aatttgcgaa tttggttttc ctaaacgttc tggatgacga atcggagcta agaaggcaag
     1861 aatatcatcc actaacttga ctttatcact tgagacatcc ttgagtcgtt ccaacaggtt
     1921 atccaaagcc tcaaaatcaa cttgtcgacc tttattatca agtaaatctt ggtgaattct
     1981 tgctagtaag tcataagcct tattgtaaaa ttctcgatca ctagatggga ggtcagtttt
     2041 cttagctcct agcttatgag ataaactttc ctgcttggcc agtttgctat caatgcctgc
     2101 tgctgtttct gctgaaagat ccttggctgg gatataacga gaaactccat tctcctcaaa
     2161 gacataacca tcgcctactt ttcgaacagc ttctttgacc aatttctcat caattggatt
     2221 gcttggagct ggttgaggat ttggtgcagg ttgcggactt ggactaggtt ccggagtcga
     2281 ttgtggactt ggttgttctg gtcttgaatc tggtacccaa tggtttgaac gataacgaag
     2341 gggaataata cgagcaattc gtttttccaa ttcagacatt tgttcatagg ggataaagtg
     2401 gtaatggtta ccatgaggga cagctacacc tctggcggtt cgacttgtga tttgcgctgg
     2461 gtcgaaaata aggccatcag attctacatg gcgttctgat aagggtttag catacaattc
     2521 acgtaaaagg cttgaaatgt tttccccttg attttgatga taagttggag tgacagtcag
     2581 attgtggttc tctgacaatc ttggttgagc tggatttgca ttatgactag aacttgaaga
     2641 aggacgagat ccctgcttcc cattccaata ggcttgggca gcagctaatt cactggcaga
     2701 taaatcactc ttaggaatat agtggaagtg attgccatga ggaacgatat aggcatcgcc
     2761 cgtatcttcg atgatatcag atgcattgaa gatataacca tcatccgttg tatagcgtcc
     2821 ttgggctctg gctgcaacta ctgcttgatc gttagaacca cccccgtgat tatgactgtg
     2881 ttcctgcttc tgacgtttaa tctcttcttt tgtccgaat
//
LOCUS       DAVLTM010000158         2822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLTM010000158 DAVLTM010000000
VERSION     DAVLTM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.159"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(134..1333)
                     /locus_tag="VYN16_001962"
     CDS             complement(134..1333)
                     /locus_tag="VYN16_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404439.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGILNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEV
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(1330..2223)
                     /locus_tag="VYN16_001963"
     CDS             complement(1330..2223)
                     /locus_tag="VYN16_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404440.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            2340..2420
                     /locus_tag="VYN16_001964"
                     /pseudo
     CDS             2340..2420
                     /locus_tag="VYN16_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT          854 a          625 c          482 g          861 t
ORIGIN      
        1 aatattatcc tattccagat tcagttcact atacaattga gttttcaagc aacctgttta
       61 cataatgtgt acataattag gttcgtgatt ccaccctttt cacctttaaa aacctcgctt
      121 tcgcaaggct cttctattta taagataagg cacgtttaaa ggttttccaa atccctaaat
      181 catccgtttg aagaacgaga ctagcataca tgcgtccgat aaatcctgtt gctaccaccg
      241 caaaaatcac tgtaatagca agtgaaatcc atgcttctgc tccccccgca tagtcattaa
      301 tcgttcgaaa cggcataaag aaggtcgaaa taaagggaat ataagaacca atcttcaaga
      361 ggagattgtc accagctgca cctagagctg tcactccaaa aaaaccaccc ataatcaaaa
      421 tcatcaaagg cgacaaggct ttccctgagt cctcaggacg agaaaccata gatcctagga
      481 aggctgccaa gactacgtac atgaaaagac tgatcaaaat aaagagcaag gtattcagtg
      541 agatagcatc tcccaagtga accaaaatac cagactgagc caagaatggc aaatctttaa
      601 agagcaaaac ggcagccaga ccacctacaa catagatccc aatatgcgtt aaaatcacta
      661 gaaacagagc catcatccgc gcatagaaat agtgacttgc ccttatgcta gaaaaaacga
      721 cttccataat tttggtgcct ttttcactgg caacttcctg agctgttaca cccgcatagg
      781 taatcagaat catataaaga aagaatccta aggcacctgc tgcaattgtt tgaataaact
      841 ttttattttc cttgacttca tcaatctttt ctgtgaattg aattgtctgc gctaagcgtt
      901 tttcctgctc ttgagacaag gaagcagttg aacgattaag ctgattttgc agttcattga
      961 gtatacctgt aacctcaaat ttaattccat tttcaagcga tgtttcgcca tgataaactg
     1021 cctttagaac actatcttct tgatcaatgg tcaaataacc ttttaatttt tcttctttaa
     1081 ttgcttcttt ggcacttgct tcatctttat agtcgaagtt aacaccattt acattcttca
     1141 gtccttctgc tacagatggc actgttgtca ctactgccac tttattattt ttagccatag
     1201 aagaaccttg gagatgccca attcctacag agattcctaa aaagaggaac ggcgaaatca
     1261 ccataaagaa gaaactccat gactcgacat gtcgaagata ggtttccttg attacaaccc
     1321 acatatttct catacttcca ctcctgattc tagtttaaag atttcatcga tagttggcgc
     1381 ttgttggtca aatgttgcga tatattgacc ttgagtcaag attgggaaga gttcccttcc
     1441 agcgctctca tcctccaaaa tcaatttcca actgccttgt ttggtcaagc tcacctgttt
     1501 gacatgagga agattttcca attcttcctt gcttcgttca cttgaaacaa agagacgcgt
     1561 tttcccgtat tgattgcgga catcctgaac tggtccgtgc aagaccacac ggccatctcg
     1621 gatcatcaga atatcgtcac aaagttcctc aacattggtc atgacatggt cagaaaagat
     1681 aatggttgct ccgcgctctt tttcctgaaa aatgacttgt ttgagcaatt ctgtattaac
     1741 tgggtccaat ccactaaaag gctcatccaa gataatcagg tctggttcat gaatcagagt
     1801 aataatgagc tgaatcttct gctgatttcc ttttgacaga ctcttgattt tatctgtcag
     1861 ctttcctttc acttccaacc tcttcatcca ttgagggagt ttttctttga cttctttggc
     1921 atccatgcct tttagagtcg ccaagtagcg aacttgttca agaactgtca atttaggcat
     1981 gagactgcgt tcttcaggca gataaccaat ccgagcatag gtctcctgac gaatatcctg
     2041 accatccaga ccgatttctc cctgatattc taggaatttc aaaatactat ggaaaatcgt
     2101 tgtttttcca gcaccatttt ttccgactag tcccaaaata cgacctggtc gcgcttgaaa
     2161 gtcaatacca aacaaaactt gcttggatcc aaaacttttc tctagacttc ttacttctag
     2221 catctttcac ctccgaaatt tcttgcactc attatactcc tttttgatag cctttacaat
     2281 gttttttgtc catttttaga agactattgc tgtgtaaaat atggcctgga gcacttttat
     2341 actcaatgaa aagcaaagag caaactagaa agctagccgt aggctgctca aagtacagct
     2401 ttgaggttgc agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
     2461 gaactgacga agtcagtaac tatacgtacg gcaaggcgaa gctgacgtgg tttgaagaga
     2521 ttttcgaaga gtattagtga taaatccatt atacagtagc aaacttaatt tataccttcc
     2581 gctcctcaac tgtctatttt taatcctgaa ttgttatttg agtaactcct ttttcctcat
     2641 aaagttttct tcctctaaaa cttctggaaa aaggcgaata gtttcagaca acatttttat
     2701 aagaaacaag ttcatctgtc atttcaagaa ggagtaatcc tttatctact aatggacgga
     2761 acagaattca accgcttgtc ccatatgttt tctaaggatt atatagtaaa atgaaataaa
     2821 aa
//
LOCUS       DAVLTM010000159         2756 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLTM010000159 DAVLTM010000000
VERSION     DAVLTM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.160"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            25..1185
                     /locus_tag="VYN16_001965"
                     /pseudo
     CDS             25..1185
                     /locus_tag="VYN16_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1301..2104)
                     /locus_tag="VYN16_001966"
     CDS             complement(1301..2104)
                     /locus_tag="VYN16_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1404441.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLIAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          954 a          496 c          483 g          823 t
ORIGIN      
        1 ataaacaatt ctaatcttag gtacatgatt aaattgattg atcgttacgg aatagagttc
       61 ggcaaaaaag gaaaaaatcg ttactattct cctgatttaa aacaagaaat gattcataaa
      121 gtctgacatg aaggctggac taaagataga gtttctcttg aatacggcct cccaagtcgt
      181 acgatacttc ttaactggct agcacaatac aggaaaaacg ggtatactat tgttgagaaa
      241 acaaaaggga gagtacctga gagcggagaa tgccatccta aaaaagttaa gagaactccg
      301 attgaaggag gaaaaagaga aagaagaaag acagaaattg ttcaagaatt aatgactgag
      361 ttttcgttag ttcttcttct aaaatccatt aaactagctc gttcgaccta ctactatcac
      421 ttgaaacagc tagacaaaac agataaggac caagagctta aagctgaaat tcaatccatt
      481 tttatcgaac acaagggaaa ttatgcttat cgtcggattt atttagaact aagaaatcgt
      541 gcttatctgg taaatcataa aagagttcaa ggcttgatga aagtactcaa tttacaagct
      601 aaaatgcgac agaaacgaaa atattcttct cataaaggag acgttggcaa gaaggcagag
      661 aatctcattc aaggccaatt tgaaggctct aaaacaatgg aaaagtgcta tacagatgtg
      721 acagaatttg ccattccagc aagtactcaa aagctttact tatcaccagt tttagatggc
      781 tttaacagcg aaattattgc ttttaatctt tcttgttcgc ctaatttaga acaagtacaa
      841 acaatgttgg aacaggcatt cacagagaag cactacgaga atacgattct ccatagtgac
      901 caaggctggc aataccaaca cgattcttat catcagtttc ttgagggtaa gggaattcaa
      961 gcatccatgt cacgtaaggg aaacagccaa gacaacggta tgatggagtc cttctttggg
     1021 attctgaaat cggaaatgtt ttatggctat gagaaaacat ttaaatcact taatcaattg
     1081 gaacaagcta ttgtagacta tattgattac tacaacaata aacgaattaa ggtaaaacta
     1141 aaaggactta gccttgtgca atacagaact aaatccttcg gataaattat ttgtctaact
     1201 ttttggggtc agtacaccta aaactttgat gatatacgtt ttcttgtgag aatatttact
     1261 tcatttttgc ctaaaattca atgtttactc agtatttgga ttatgaaaaa tcgaggtcta
     1321 aatctagata cattttttct gaagacaaat cattttgacc accgagcaag agattttcaa
     1381 aaaaagctgt taaaaactca ggacgtcgct gtaaaatctt tgcattatct aataccaagg
     1441 catcacgaaa atacttggaa tgttgctgaa atggtatatt atcaatatca aaaccaaact
     1501 cacgaagata ctgaatcaaa aagaccgtta ctgtccgagt gtttccttcg cgaaatggat
     1561 gaatctgcca gattcctgaa ataaaatgct ggatttgttt aaccacatcc gcctgagtta
     1621 gtgtcgcata tgcaacttgt ttttcctgat taaaatcata atccaaggtc atttgaatca
     1681 tggagtaatc agagtacaca acactttcac cattcaaaac aggttcattc tttgtgatat
     1741 tggtctgacg aaattgcccc accggaatag agggttcaaa tatatcttga aacaactcct
     1801 tatgaatagc aattaaagtc gcaggactga atctaaaacc tcttcgagac aagagttcta
     1861 cgatacgcaa agaaaccaag tctgcctcct ccgtactcgc atcaacggta tgatgataaa
     1921 ccgttgcatc ctcataaacc tgctcataag tcagttcacc ccgggactgt ttctcagcca
     1981 aagattccat atacgctgat ggcactagat tgtcaacttt ctgcagacca aaacctatcc
     2041 gccataaatc acgcttcgct tcataagaca agtttggatt gtcaatgttg taagttgatt
     2101 gcatagaaat atcctttcaa ttgtttttct gtgtcatcgg taaattgtga aataggtatg
     2161 gaattggcga aaattataaa aatccttcac aataggttct atgaaatata tatacttcat
     2221 cttaatagtg agcaagctaa acttagcatt tcatgccctc atatgggatg ttctttgact
     2281 aaataatatg attatcgaga tatatctgga taaatgaact aataagtctg acgcgtagac
     2341 ttatcaaagt cattggcata caccactatg aactcgttgg tctgttcaaa tcccaacaca
     2401 ttacctgaga agaaagttgc aatgttgttt ttggtgcggg tttgaattta aaaatttgtt
     2461 atgtagtacc taatctaagg aattagaaca atgcctctaa tttttcttta atacactgaa
     2521 acattgatga ttctggctgt atttttgaaa cagctcttct ttgctcctgg aaaatatctt
     2581 cagaagttat attctctatt cctaacgcta cttgaatttt tttctaaaat attcttttcc
     2641 gttgccatct ttagaaaaat cataaccttc cctatctacg ctgttacata aattagctaa
     2701 aaaaaactat ggggttggga aaggaagata agaaacgtat ttagcccaat aatcta
//
LOCUS       DAVLTM010000160         2700 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLTM010000160 DAVLTM010000000
VERSION     DAVLTM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.161"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            313..498
                     /locus_tag="VYN16_001967"
                     /pseudo
     CDS             313..498
                     /locus_tag="VYN16_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            780..938
                     /locus_tag="VYN16_001968"
     CDS             780..938
                     /locus_tag="VYN16_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404442.1"
                     /translation="MIEDFLIDLENIQLEDMEIDFNMEIDIDLNIDFEIDVDLDGLDL
                     TLLQELEA"
     gene            complement(1370..1564)
                     /locus_tag="VYN16_001969"
     CDS             complement(1370..1564)
                     /locus_tag="VYN16_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1404443.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(1629..>2700)
                     /gene="dnaN"
                     /locus_tag="VYN16_001970"
     CDS             complement(1629..>2700)
                     /gene="dnaN"
                     /locus_tag="VYN16_001970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1404444.1"
                     /translation="SSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
BASE COUNT          893 a          468 c          509 g          830 t
ORIGIN      
        1 aatattaagt tcctttaatt ttagtccgat tttttaaaaa gaattactac aaattgatac
       61 taaaaaggta caaaaaaggg gggtaatcgt cgaggtaaag gggggtaatc gtcgaggtcg
      121 cgaatttgta aacccttgat attactgact ttctaagggg tcaaaatttt caatactatt
      181 aaaactatta agactattaa agagcacccc ccactctttt aagaagtggg ggtatgctat
      241 aataaaacca gtaaaaagaa aaggcgggag cggacatcac tttttcttta gaggcactca
      301 agagaggtaa gcatggtaac agacattgaa caagcaagaa agaactatat ggcagactat
      361 acgccagtag accttacaga cgcacacaaa ctactagaac tagctgaaaa gctacaaaac
      421 gaggatataa gtatcaacac ttacgagtta tacaaacatc cagaagcaag agagaaatta
      481 ttttggcagg ttgctgaaga aatagatagg tcaaagacta cagtatacct tataaggcaa
      541 aaagccatag aacacttagc aaatgtgggg taataggtga gcacaaaaaa acgcctagaa
      601 caatctaagc gctaaagagt agtggacggt gtgcctgtcc cgtcatctca tactatgaag
      661 ttgcgtagcg acactatcat ctctacctca cttctcttta aactaattat accacaaagg
      721 agcgaactaa tggagctgga taagtttaaa acgatgatga acgtcagaga gcggatgaca
      781 tgattgaaga ttttttgatt gatttggaga atatccaatt agaggacatg gagatagatt
      841 ttaacatgga gatagacatc gatttaaaca tagattttga aatagatgtt gaccttgatg
      901 gtttagattt gaccttacta caagaattag aagcgtaaat gttggtaaat gtaggctgag
      961 gcttcatatt cgcctcccta tttcgcttgg tttcgtaccg gacgattaaa acaccatgga
     1021 taaactagaa gcccttagaa acgaatcagg gggacttttg tgaggtcata atcttataaa
     1081 ccacgcgctt catggtataa tatacctatc agcaaccagc aataaaaaaa gcacgtttga
     1141 acgtgctagt ttcttgcctg ctgaactcat cgatttagta gcccttttgt gggctttttt
     1201 tgtggacttt ttgagaattt ttaagaactt ttaatgattt ctaatcaaat gagatttttc
     1261 aaaaagtcag taatatcaag ggtttaggcg ctataaacaa atttaaaagt gtgtagatta
     1321 caatctaaca caaaaaggga taaagtgcta accctctaac taagggttct cagtcaataa
     1381 ttttattcat ttttcgctca aaatcatatc gccccatcat gacaacatgc tcacaattac
     1441 tgcattttat tttgatatct gctcctacac gtgtaatttc ccaacgatta gcctttttac
     1501 cagtcgactt gattgtacaa gcgtgtgatt ttttcatctc aacaaaattt ccaacttgat
     1561 acatactact ctccttttct ttttcgatta tatcataaaa gaggcgagcc aggctcaacc
     1621 tctttcactt aatttgtacg aactggtgta atgagctgca tgaagtcttc gtcagtatct
     1681 gctggcacaa gagtaaatgg acgaacagct gagataaagc taatggtcac cttttcgcta
     1741 tttaaagctt taagagaatc aatcaagtaa gttgggttga aactaatggt caaatcttca
     1801 ccagtaacct gatcagtatc gatttcttcg tttactttac caacttctgg agagtgaaca
     1861 tgggcgctaa caaccccatc cttaatttca agtttcacag taccattttg agtcgcactt
     1921 gataaaagac gggcacgctc cattgactgg cgtaagttta ccacattaaa agtaatagta
     1981 gtgttaaagt ctgttggaat caagcgatct gtatcaggat agtttccttc taggagacga
     2041 gtatagaagc taatattttc gcttctaaag aggatttggt tattggcaaa gaaaatctct
     2101 acagtttcga tatcatctgt aaataccgct gaaaattcgc gtagagaacg gctaggaatt
     2161 acgacatcaa aatcatcact atttttttca agagtcaatt ttttctggct taggcgatga
     2221 gagtctgttg caactgtttt taactctttg tgttgactca atacgaagtg gacacctgtt
     2281 aaaatcggac gactctcttg tgtacttgca gcaaaggctg tttcattaat aattttcttg
     2341 agtaattttg tttcaagtat taaaggagtg cttgctgaaa tttcttggat tcgtggatat
     2401 tgttcgctat cttttccttt tagggtaatt tctgatttgc cactggttaa aacaatttga
     2461 ttttgttcaa tttctttaaa atcaagagtt acatcaggta aactagatac tacattgata
     2521 aagaaagaag cttcaagaag gatcgaacct aaagaagtaa ttaacaaacc agcatcttca
     2581 tttttttgag aaataaaatt ttcaattgaa atttgaccat ttgaaccaat taaagtaata
     2641 ccttcattgg tcacgtcaat ttttactgtt gataaaatag gaatggcatt tttagaacta
//
LOCUS       DAVLTM010000161         2687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLTM010000161 DAVLTM010000000
VERSION     DAVLTM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.162"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..1677
                     /locus_tag="VYN16_001971"
     CDS             <1..1677
                     /locus_tag="VYN16_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1404445.1"
                     /translation="SKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVG
                     EYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQ
                     DYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEDCIQKAREGKWNGG
                     FAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGK
                     NPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIV
                     SDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDG
                     TKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKI
                     NMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRM
                     YDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALI
                     SEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            1945..2511
                     /locus_tag="VYN16_001972"
     CDS             1945..2511
                     /locus_tag="VYN16_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404446.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1002 a          339 c          594 g          752 t
ORIGIN      
        1 tcaaaagaaa aaataaaagt atacctctat acacgagtat ctacgtcaat acagatagag
       61 gggtattcgt tagaggcaca aaaatcacga atgaaagctt ttgctattta caacgattat
      121 gaaattgttg gagagtatga agatgccggg aagtctggca agtctattga aggtagaata
      181 cagttcaatc gaatgatgga agatataaaa tctggaaaag atggagtatc ttttgttctt
      241 gtgtttaagt tatcaagatt tgcaagaaat gccgctgatg ttctttctac acttcagata
      301 atgcaggatt atggagttaa tcttatctgc gttgaagatg gcattgattc atctaaagat
      361 gctgggaaac taatgatttc tgttttatca gctgtggctg aaatagaaag agaaaacatc
      421 cgtattcaaa caatggaaga ttgcattcaa aaagcaaggg aaggaaaatg gaatggtggc
      481 tttgctccgt atggatataa acttgaagac ggcaagctgt ttataaatga ggaagaggca
      541 gttgcaataa gaacgatttt cgaccagtat gtaaatacta cgataggagc caatgggata
      601 tctaaatact tagagaatca tggaattaga aaaatcccaa gacagaatgg taagaatcct
      661 ttgtttgatg caggtcttat aagaaagata ttaaagaatc ctgtatataa tgggaaaata
      721 gcatttggaa gaagaacttt agaaaaagtt catggtacaa gaaatgaata taagcaggtt
      781 gaacaagatg aatatctaat atctgaaggg atacatgaag ctatagtttc cgatgaagtt
      841 tggcaagctg ctcaggttaa gctaaaatct caagcaaaga aatatgagca tgtgaataaa
      901 ggaaaagata cacgcacaca cttgctttct ggaattgtaa aatgcccgat atgtggagtg
      961 ggaatgtttg ggaacaagtg tatcaagaaa aagaaagatg gcacaaagta taaagatttt
     1021 tattactatg gttgtaaaca taggcagatg ataagaggtc ataagtgtac attcagtaag
     1081 caaattagag aagaattgtt agatgatgct gttgcagagg tgattgtcaa gatagtaagt
     1141 aatccgaaat ttgcttctat gatgcaagaa aaaatcaaca tgaaggtgga tacctctgaa
     1201 atagaaaaag aaatagataa ttaccaaaag gaattgagaa agagtcattc tacaaagttt
     1261 aagctaattg aggaaataga taatttagat gttgaagata agcactataa gagaaggaaa
     1321 caggatttag atgatagact atatcgtatg tatgacaaaa tagatgaatt agaatcatca
     1381 ctaattgatg cgaaagcaaa gaaacagact attgaagctg aaaaacttac aggagataac
     1441 atatataagg ttctgatcta ttttgataag ctctataaag tcatgaatga tgtagagcgt
     1501 aggcagttaa tttcagcttt gatttctgaa attcaagttt atgaagaaaa acaatctaac
     1561 ggacaatggc taaaatccat tacttttaag ctaccaatta tagaagaaaa tctcaacata
     1621 gatttggaca atgatgaaca agttgaatgt gtaagtttat tagagaaacg tagttaatcc
     1681 tcaaaaggtt ccccaaactt tttgagtccc actgacgcat cacgctgagg cggtatcact
     1741 gcttgtacga gttgaagcat cagcgaagta aaagtagatg ttcagccaat gcgacgaggt
     1801 gagaccgaag gtcgataagg tgtgttctgg gtataggact cgtaaagtaa gtaggaaata
     1861 tgatgagatg atacagagag cctgaattgg tcaggtgacg tgcttattta cattagcctg
     1921 ataatgaaaa tggagagatt atcgatggtt atattggaat tgtaccaaaa tgattattct
     1981 aaagatttag tagcatttga ttccatagaa gatgggaaag cctttgtggc tcaaatcccg
     2041 ggatacactc tggaaacaga agatggtttt gaagtggagt attttaatcc taaaaacata
     2101 ccggattata tggaaattat ttttaacgga aatattgttc ctttatctaa atttatgttc
     2161 gatcctgagg aaaatgtaaa tattatttgg aaagagattt caaatttatc cctcaaaaat
     2221 gatagagtga tcgaaggata ttcaaaaatt gatgcatatg tcgttaacaa ccatgaagtt
     2281 aaagcctatg tcgaaacaag agaaacgaac tatcgtaaag caaaagactt cttagaaagc
     2341 cgcggatatg aaatcgacag aagctttttc ggaagtgaag acggtgaagc gattctttac
     2401 agaaaaaaag ggatcgaagt ttggcatttc ttgtgtcact tagacccaat gtttgtagag
     2461 attgaagatg tagaaggata tgttaaagaa gaagttggag agattcaata gtcaaccaaa
     2521 gttccttgtg agtatatacc tttgctagta aaagagtcaa aacgttataa cgaagggggg
     2581 gaagaaaacc cttgagtgta agtctttttc ttgtttagag ggaaagtatt agagtagccg
     2641 ctcataattt atactagatt tgataactga tttgagacag attttat
//
LOCUS       DAVLTM010000162         2673 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLTM010000162 DAVLTM010000000
VERSION     DAVLTM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.163"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            35..724
                     /locus_tag="VYN16_001973"
     CDS             35..724
                     /locus_tag="VYN16_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1404447.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            721..1644
                     /locus_tag="VYN16_001974"
     CDS             721..1644
                     /locus_tag="VYN16_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1404448.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            1659..2303
                     /gene="pcp"
                     /locus_tag="VYN16_001975"
     CDS             1659..2303
                     /gene="pcp"
                     /locus_tag="VYN16_001975"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1404449.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            2471..>2673
                     /locus_tag="VYN16_001976"
     CDS             2471..>2673
                     /locus_tag="VYN16_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1404450.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEI"
BASE COUNT          816 a          396 c          588 g          873 t
ORIGIN      
        1 aaatactgtc attttgaata taaaggagtt tgatatggag tggattagat taataggaat
       61 agcaatcatt gttgtgggtt ttattttaaa atttgataca attgcaacag tagtcttagc
      121 tggtttggtt acagctttag tttcaggtgt ttctctcgtt gaatttttgg agattttggg
      181 aaaagaattt agcaatcagc gagtgctcac gatttttatg gttaccttgc ctcttgtggg
      241 gctgtcagaa acctttggac tcaagcaacg atcaatcgat ttgattcgaa agattaaagg
      301 tctgacagtt ggaaacttct atacagttta tttctttatt cgagagttag ctggtttctt
      361 ttcaattcgt ctaggaggac accctcagtt tgtcagacct ttggttcaac ctatgggaga
      421 agcagctgca gagtctcaat taggtagaaa gttaacagag gttgaagatg agacaataaa
      481 agcgcgtgcg gctgcgaatg aaaattttgg aaatttcttt gctcaaaata cgtttgtagg
      541 tgctggggga gtcctcttga tagggggaac attagatcag ttaggctatg aaagcaatta
      601 tgcagggatt gcttctacat ctattattgt tgctgttata acacttattg tagtggggat
      661 ttacaattat ttatttgata aaaaattgat atcaaaaaag actaggggag gagaacaaaa
      721 atgacagagt tggcaaagca actattagag ttgacctata ttgtgattgg ttgtcaattt
      781 ctccatacag cctattgtag ttataaagat aaaacaaacc cagttcgact tgggacatct
      841 gcattttgga ctctattgtc tattacgttt ataggtggtt cctatatgcc aaatatgagt
      901 attggtatta ttgtaatcct attatcgctg ttaacattgt ttaagcaagt ccgtatcgga
      961 accttgccat ccttagatga aatgaaagcc aatattgaat ctaacaggtt gaaaaataaa
     1021 atttttattc cagttatgct gatggcaata cttgcgttgg tcttagcgca aatgattcca
     1081 gaatttagca agatttcgat tagccttgcc gccttgtttg ctacaatttc tgttcttgtg
     1141 attaccaata gtcaccctaa gagtctgtta tcagaaaata atcgaatgac tcagcaagtt
     1201 tcaacaagtg ggattgttcc tcaattatta ggggctttgg gggctatttt tactgtagca
     1261 ggtgttggtg atgttatctc tcatctgatt agcggtattg ttccttcaga tagtcgcttt
     1321 ataggagttt tggcttatgt tcttggaatg gttctattca caatgattat gggaaatgct
     1381 tttgcagcat tcaccgttat tacagcaggt gttggagttc cctttgtatt tgctctggga
     1441 gctaatccaa ttgtggctgg tgctcttgcc atgacagcag gttattgtgg gaccttattg
     1501 accccaatgg ctgctaattt taacgctcta ccagcagcat tgatggatat gaaagatcag
     1561 aatggcgtta taaaggctca agcaggtgtt gctctagtaa tgattgttat tcacatattc
     1621 ttaatgtact ttctcgcatt ttagtaaagg aaaataaaat gaaaatattg gttacaggtt
     1681 ttaatccttt tggaggtgaa aagattaatc cagctttgga ggctgtaaaa ttattaccat
     1741 ctgagattaa tggggctgaa gttcgctggg tagaaattcc aacggttttt tataagtcgt
     1801 cagaagtttt agaggcagaa atattacgat atcaaccaga tgctgtactt tgtattggac
     1861 aagcaggcgg caggaccggc ttaacacctg aacgagtggc tattaatcaa gatgatgctc
     1921 gcatacctga taacgaaggc aatcaaccaa ttgatacacc gattcgtatt gatggagcat
     1981 cggcctattt tagtagttta cctatcaaag cgatggtaca agctatcaaa aaacagggac
     2041 ttccggcagt tgtatccaat agtgcaggaa cctttgtttg caatcatttg atgtaccaag
     2101 ctctttattt agtggataaa aaattcccta atatgagagc aggtttcatg catattccat
     2161 atatgatgga acaagtagta aataagccga atactgcagg aatgagtcta tgtgatattg
     2221 taagaggcat agaggtagct attgaagcta ttgtagacta caaagataag gatttgcagt
     2281 tagtaggcgg tgagactcac tgataaccta aaaaggatag tcaattatgc ttgtttacta
     2341 actattaact atgctaaatc aattgaggtt gtttacataa aactctatat cagagaagcc
     2401 tgatatagag ttttttcttg ctagttttag gattttcttg taaaatagaa aaagtgaaga
     2461 gaggtatgaa atgagcaaga aagataaaaa aatcgaaatt caagtagcgg atgccaaagt
     2521 taatgttggt aaagacagtt ttgaaggtta tacattgact atcggtaaaa aagttatcgg
     2581 agaaattgcc gaattagacg gacaatttgc cattataaag aatgggaatg tcgatagttt
     2641 ttataaaaaa ttggaaaaag ctgtggaaat ttt
//
LOCUS       DAVLTM010000163         2670 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLTM010000163 DAVLTM010000000
VERSION     DAVLTM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.164"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..674
                     /locus_tag="VYN16_001977"
     CDS             <1..674
                     /locus_tag="VYN16_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEV1404451.1"
                     /translation="NKEKAKRRTFDELTPLEQEQRLERMKKTRLEAKWNLLRLVDCNF
                     DDTTSFLTLTTKENISDRNHFKDLLKTFIKRFNYHIFNTKKSKLKYIAVLERQKRKAW
                     HSHILLFSVPYIPHKQLLELWGHGAVRINKVDVDSKENRGRYVTKYFEKGIGQELLEN
                     FGKQAYFSSRNLKKPDEDKFYTYEDFNYDSSVVLYETEYTSKVYKDGQYFDNHVKYKK
                     IRLDE"
     gene            688..891
                     /locus_tag="VYN16_001978"
     CDS             688..891
                     /locus_tag="VYN16_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV1404452.1"
                     /translation="MIATVTKNDLIALGFSEGTSKRIIRQGKELLITRGFTVYQNKRI
                     GTIPATIATELLGFDVQNSTLKE"
     gene            895..2028
                     /locus_tag="VYN16_001979"
     CDS             895..2028
                     /locus_tag="VYN16_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1404453.1"
                     /translation="MAIRKGKDGNWVVDISNGFDPITLKQRRLVRKGYKTKKEAIEAE
                     HYLRSVELKERFYGAKITISILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     DNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYSSN
                     PAKLIKKLPIEKTKMQFWTVKEFQKFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSVYVNKGVTHISTTKTKAGTRRIVINKKLSQELQDWEKRQKHL
                     LEQFTNNSLTLQVFQNSPITITKNAIEKQYKKIIERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            2069..2506
                     /locus_tag="VYN16_001980"
     CDS             2069..2506
                     /locus_tag="VYN16_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1404454.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVASFVATDEE
                     KAVAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
BASE COUNT         1077 a          441 c          443 g          709 t
ORIGIN      
        1 aaaacaagga aaaagcaaaa cgcagaacat ttgatgaact aacaccccta gaacaagaac
       61 aacgtcttga acgtatgaag aagactcgct tagaagccaa gtggaactta ctccgtctag
      121 ttgattgtaa ttttgatgat acaacttctt ttcttacatt gaccactaag gaaaatatca
      181 gcgatagaaa ccacttcaaa gacttactca aaacatttat taaacgcttt aactatcaca
      241 tttttaatac caagaaaagt aaattaaaat atatagcagt attagagagg caaaaacgta
      301 aagcatggca tagtcatatt ttgctattca gtgttcccta tattccacat aagcaattat
      361 tagaactttg ggggcatgga gcagtacgga taaataaagt tgacgttgac agcaaagaaa
      421 atcgcggtcg atatgtcaca aagtattttg aaaaaggtat tggacaagaa ttgttggaaa
      481 acttcggaaa gcaagcatat ttttcatcac gcaacttgaa aaagcctgat gaagataaat
      541 tttacactta tgaagatttt aactatgata gttcagtcgt actatatgaa acggaataca
      601 ctagtaaggt ctataaagac ggtcagtatt tcgataatca tgtaaagtac aagaaaataa
      661 gactagatga ataaaggagt aagtcacatg atagcaactg ttactaagaa tgacctaatt
      721 gccctcggtt tctctgaagg aacatctaaa cgtattatcc gccaagggaa agagctactt
      781 ataacccgag gctttacagt ctatcagaac aagcgaattg gtactatacc tgctactatt
      841 gctaccgaat tactcggttt tgatgttcaa aacagtaccc tcaaggagta aaatatggca
      901 attagaaaag gtaaagacgg taattgggta gttgatatta gcaacggttt tgacccaata
      961 acactaaagc aacgtagact tgtcagaaag ggctataaga cgaaaaaaga agctattgaa
     1021 gcagaacact acttacgaag tgtagagtta aaagaacgct tctatggggc taaaataact
     1081 atctccatac tatatgaact acttaaggaa gaagatagca taaatcatcg aaaagcaagc
     1141 tatatcaata cacaagataa taattacaac aaacacatca aagattattt ttctatggtc
     1201 gataatgtcg gaaaactaac ctatgaagac atctaccaat ttagagaaca tcttaggcaa
     1261 aaagtagcta aaaattctga taaaccgtta agcacaaata ctattaacaa aatcatgatt
     1321 ttactcaaaa aaatttttga tgttggtttg cgaaaaggct attactcctc caatccagca
     1381 aaattgataa aaaagttgcc aattgaaaaa acaaaaatgc aattttggac agtcaaagaa
     1441 tttcagaagt ttttgacact atttgaaccc gaagaataca atatcaaact actctttact
     1501 gttctattct ttacaggatt aagacttggg gaagctcttg ccctaacatg gcaagacatc
     1561 gacttttcaa gtaatacaat tcatatcacg aaatctgtct atgttaataa aggagtaact
     1621 catatcagca caacaaaaac taaagcagga acaagaagaa ttgttatcaa taaaaaatta
     1681 agccaagaat tacaagattg ggaaaagcga caaaaacatc tattagagca atttaccaac
     1741 aatagcctaa cattacaagt gtttcaaaat agtcctatca caattacgaa aaatgctatt
     1801 gaaaagcagt acaagaaaat cattgaacgt gatagcacac taaaaaagat acgcatacac
     1861 gattttaggc actcacacgc ttccctgctg attaaccaag gggaagatta tctagttgta
     1921 aaagagcgac taggacatgc ttctataaca accacgattg atacctactc tcacttgtac
     1981 ccaagtaagc aaaaggattt agctgataaa ctagatgatt tactataaag taattgagtt
     2041 ttataaatgg aaagtttata attaaatcat ggatacatca caagtaataa aagcacatga
     2101 agtagctaaa aaagcacact ttggacaaat tgatagagca ggaattgact acattaaaca
     2161 ccctgaaaca gtagctagtt ttgtcgcaac tgatgaagag aaagctgtcg cctatctaca
     2221 tgatgtaatt gaagatactt cactaaccct actagaccta aaaaaggaag gtttttcaaa
     2281 aaacattata gaagccgtcg atatactcac aaagaaaaaa ggacaagatt atcaatctta
     2341 tctcaatcta gtaaagaaaa atgaactcgc tagagttgta aagttggcgg atttacggca
     2401 taattcagat ttaacaagac taccattaat tactgaaaaa gacttagaga gaaataagaa
     2461 atacagttct gctattagat ttttaagagc aaagaaaaaa agttagtaac taaactataa
     2521 aattttataa agagaaaaca aaagacaagg cttataaact ttgtctttat tactatactg
     2581 gcggtcggtc tcgaactggc acatccttgc agatactaca attttattaa aataaataac
     2641 tataaaaagt gaaatttttt attgagaaac
//
LOCUS       DAVLTM010000164         2660 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLTM010000164 DAVLTM010000000
VERSION     DAVLTM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.165"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            251..565
                     /locus_tag="VYN16_001981"
     CDS             251..565
                     /locus_tag="VYN16_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404455.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            575..781
                     /locus_tag="VYN16_001982"
     CDS             575..781
                     /locus_tag="VYN16_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1404456.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            781..978
                     /locus_tag="VYN16_001983"
     CDS             781..978
                     /locus_tag="VYN16_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404457.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            1042..1716
                     /locus_tag="VYN16_001984"
     CDS             1042..1716
                     /locus_tag="VYN16_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1404458.1"
                     /translation="MFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTFNF
                     LKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEVII
                     LHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEGER
                     QANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQLPM
                     VKRYFK"
     gene            1728..2369
                     /locus_tag="VYN16_001985"
     CDS             1728..2369
                     /locus_tag="VYN16_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1404459.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            2387..2617
                     /locus_tag="VYN16_001986"
     CDS             2387..2617
                     /locus_tag="VYN16_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404460.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          778 a          436 c          546 g          900 t
ORIGIN      
        1 actgttttga ggttgtagat agaactgacg aagtcagtaa ccatacctac ggcaaggtga
       61 agctgacgtg gtttgaagag attttcgaag agtattaatg atagataaag tggtcaggaa
      121 cttactcctg acctttttct tttgcaaaat gacaaaaatc ataaactttt tgacaaatat
      181 acttgtcaaa aagaaaaaga tatgttacaa tgaattcaag ttaagaaata ggaagcagaa
      241 aggagttata atgtgggtac taggatttat actatttatg attttctttt attctaataa
      301 ttctaaaaaa atcaagaaac tagagaataa aatcaaaaaa cttgagcgaa aagagaaagg
      361 aaacgcagaa atgtcgagat tattacaaga aatgattgga aaggaaccaa ttataacggg
      421 agtgtatatt gggccagata actgggaagt tgtggatgtt gatgaggaat gggtcaaact
      481 acgtagtgtt gataatacgg gaaaagaaaa attcaagttg caacgtattg aagatatcca
      541 aaccgttgaa tttgacggag agtaggtgtg taacgtgcaa ctaaaaaatc gtctaaaaga
      601 gcttcgagct cgcgatggtc tcaatcaaac cgacctagcc aaactggcag gggtttccag
      661 acagaccatt agcctactag aacgggatga gtacacccca tccattatca ttgccctgaa
      721 aatatctcaa attttcaacg aaacagtcga atcggtattt cgtttggagg aggacgagtg
      781 atgaacaagt ataaagtgat ttattatgta gtggtcatag ctttattagt cagcgtattt
      841 ctactgattg ggatggacct aagctggttt aatccctatc aaagcgacca atttgtttgg
      901 gtctactttg ctctcatccc agtaattgaa tggattgaaa agaaatccaa aaatctagca
      961 agtgaaaaag gagaatgaat atgaaaaaga aacgtagatt gttatttttg atgtctattg
     1021 tcttgggggg gggtcttggg catgtttgta gggatgttta aggcacgtgt cgaatcccac
     1081 gaaattattt tagatgtaaa agccttgatg ccatggatat cagctatttg tttactgata
     1141 ggtttcatta gtatgttttt gactttcaat ttcttaaaga aaagcagaaa atttcattcc
     1201 ttgtatcaag aggaaatgga tgacgatctg aatgaaacct attatgtgca aatgtatcgg
     1261 aatcttgagt ttggaaccat tgcttttaat attacaggtg tagcgattcc attggctatt
     1321 tttatttcat taagtgaggt gattatattg catacaaacc ctcaaacatt tttcctttct
     1381 ttcttactct ttgtggtatt cttagtcgct caaaaatctc tttttaaaac cattgcgatt
     1441 gttcgtcagt ttgatttgga atttttcgct acaccaaagg atgtcttgaa ctatataaat
     1501 tcttatgatg aaggggagcg tcaggctaat ttggaacaga gttttcgaat tttattccaa
     1561 ttacaccaat atgtcttacc agccttatat atttttctta ttatcctttc tttcttgaca
     1621 ggagagattc agttactagc tttcttgctt gtaggagcca tccatgttta tatcaatgtg
     1681 atgcagttac ctatggtaaa acgttatttc aaataaagga gttatctatg aaattactta
     1741 aaaatcttgg ctggtttctt ctagcccttc tatccttttt ctttatctat ggctttattc
     1801 aggggctcgc gactgcgtcg cttgctttag gcgcttcacc ctatgctgtt actctactct
     1861 atgtggtctt ggctggagta tatgtgtacg gcatttacaa atggtatcag aaagctcctg
     1921 tttatattga gaagagcggc tttaaccgat ttatttggct tcctgccttg gtttggttct
     1981 tatctctggt cgttcaattc ttcttgccag atgatccttc agtaaatcaa caaatagcga
     2041 cagacttgac cttgtctcaa ccacttttct cattctttgc tgtggttatt tttgctcctt
     2101 tgacggaaga gattgttttt agagggatgt tagcacgcta tctctttcct aagcaggaca
     2161 atagtaaacg aaccctgatt tttcttctgg tatctagtct tctatttgcc ttgattcatt
     2221 ttccaggtga tgtgcaacaa ttttttgtct attttagcct tggttttagt ttgggtttgg
     2281 cttacgttag cagaaaaggt ctggtctaca gtatttctct tcacgctttg aataatttag
     2341 tcggcttttt gatgattctc atgctataat agagtcagga ggtcacatga aacgagtaat
     2401 tttattagca gtgattcagg cagtcgttct atttttcatc attggagcgc tagcttatgc
     2461 cttcaaaggc gatttcttct ataactatct agcagttgtc tttgctccta ttgcaggtgt
     2521 actgcgtttt gggacggctt acataacgga aattgtcttg cctcgaaagg cagccgaaat
     2581 cgctgaaaag cgtaaagcag gcaaaaattc aaaataaaag agtccggtga agttcatcgg
     2641 atttttgtat tatactcaat
//
LOCUS       DAVLTM010000165         2528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLTM010000165 DAVLTM010000000
VERSION     DAVLTM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.166"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            112..609
                     /locus_tag="VYN16_001987"
     CDS             112..609
                     /locus_tag="VYN16_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1404461.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            609..2264
                     /gene="dnaX"
                     /locus_tag="VYN16_001988"
     CDS             609..2264
                     /gene="dnaX"
                     /locus_tag="VYN16_001988"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1404462.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVCLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2292..2486
                     /locus_tag="VYN16_001989"
     CDS             2292..2486
                     /locus_tag="VYN16_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1404463.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
BASE COUNT          745 a          466 c          552 g          765 t
ORIGIN      
        1 aatatccata ggggatttac ccactacaaa tattatagag cccaaaatcc tttgtttact
       61 aaacaaggga tttttctttt gtctctgctc cttttttgat ataatagttc tatgttaaaa
      121 tcagaaaaac aatcacgtta tcaaatgtta aatgaagaat tgtccttcct attggaaggc
      181 gaaaccaatg ttttggctaa tctttccaac gccagtgctc tcataaaatc acgttttcct
      241 aataccgtat ttgcaggctt ttatttgttc gatggaaagg aattggtttt aggccccttc
      301 caaggaggtg tttcctgcat ccgtattgca ctaggcaagg gtgtttgtgg tgaggcagct
      361 cactttcagg aaactgttat tgttggagat gtgacgacct atctcaacta tatttcttgt
      421 gatagtctag ctaaaagtga aattgtggtg ccgatgatga agaatggtca gttacttgga
      481 gttctggatc tggattcttc agagattgag gattacgatg ctatggatcg agattatttg
      541 gaacaatttg tcgctatttt gcttgaaaag acagcatggg actttacgat gtttgaggaa
      601 aaatcttaat gtatcaagca ctttatcgaa aatatagaag tcaaaacttc tcccagttag
      661 ttggtcaaga agttgtggct aagactctta aacaagcggt ggagcaagag aaaataagtc
      721 acgcttatct tttttctggt cctcgtggaa cgggaaaaac cagtgttgct aaaatctttg
      781 ccaaggctat gaactgtccc aatcaagtgg gtggcgaacc ttgcaataac tgctatattt
      841 gtcaagcagt gacggacggt agtttagaag atgtcattga aatggatgca gcttctaata
      901 atggggtaga tgaaattcgc gaaattcgtg ataaatctac ctatgcgcct agccttgctc
      961 gttataaggt ttatatcata gatgaggttc acatgctgtc tacaggggct tttaatgccc
     1021 tcctaaagac gctggaagaa ccaacacaga atgtagtctt tattttggcc actactgaat
     1081 tgcacaagat tcctgctact attctatccc gtgtgcaacg ttttgagttt aaatcaatta
     1141 agacacagga tattaaggaa catattcact atatcttaga aaaagaaaat atcagttctg
     1201 aaccagaggc tgtggaaatc attgccagac gggcggaagg tggaatgcgg gacgccttgt
     1261 ctattttgga tcaagccctg agtttgacac agggaaatga gctgacgact gctatctctg
     1321 aagaaattac tggcaccatt agcctatcag ccttggatga ttatgtggcg gccttgtctc
     1381 aacaggatgt tcccaaagct ttgtcttgct tgaatcttct ttttgacaat ggtaagagca
     1441 tgactcgttt tgtgaccgat cttttgcact atttaagaga cttgttaatt gttcaaacag
     1501 ggggagaaaa tactcatcat agttcagtct ttgtagaaaa tttggcactt cctcaaaaaa
     1561 atctgtttga aatgattcgc ttagcaacag tgaatttagc agatattaag tctagtttgc
     1621 agcccaagat ttatgctgaa atgatgaccg tctgtttggc ggaaatcaag cccgaaccag
     1681 ctctatcagg agcggttgaa aatgaaattg ctacgctgag acaggaagtt gcccgtctca
     1741 aacaagagct ttctaatgca ggtgcggttc ctaaacaagt tgcaccagct cctagtcgac
     1801 cagctacggg caaaacagtc tatcgtgtcg atcgcaataa agtgcaatct atcttacaag
     1861 aggccgtcga aaatcctgat ttagcacgtc aaaatctaat tcgtttgcag aatgcctggg
     1921 gagaggtaat tgaaagtcta ggtgggccgg acaaggctct gctagttggt tctcaaccgg
     1981 ttgctgccaa tgaacaccat gctattcttg cttttgagtc taacttcaat gctggtcaaa
     2041 ctatgaaacg agacaatctc aataccatgt ttggtaatat cctcagtcag gcggcaggtt
     2101 tttcacctga gattttagct atttccatgg aggaatggaa agaagttcgc gcagcctttt
     2161 cagccaaagc caaatcttct caaactgaaa aagaagtaga agaaagcctg attccagaag
     2221 gatttgaatt tttggctgat aaagtgaagg tagaggaaga ctaaagaaag atttcatgat
     2281 acaataagtt tatgaataaa caacaattta ttattatggc gctatttaca gctgctgaga
     2341 cctatttttt caatgaagcc tggatgactg gccgctatat tatggcagcc ttttgggcaa
     2401 ttttactctt tagaaatttc cgagtcagtt atgtgatggg taaaattgtt gatgtcatcg
     2461 atcagcattt taataggaaa gactagccct cagcttccag acaaaatcaa agccttttag
     2521 gctttttt
//
LOCUS       DAVLTM010000166         2514 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLTM010000166 DAVLTM010000000
VERSION     DAVLTM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.167"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..591
                     /locus_tag="VYN16_001990"
     CDS             <1..591
                     /locus_tag="VYN16_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1404464.1"
                     /translation="FGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKN
                     VTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTIRGTVGNVAYYDELIKYKHLRIN
                     SGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN
                     EFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            651..887
                     /locus_tag="VYN16_001991"
     CDS             651..887
                     /locus_tag="VYN16_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1404465.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            884..1297
                     /locus_tag="VYN16_001992"
     CDS             884..1297
                     /locus_tag="VYN16_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1404466.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSITVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            1415..2380
                     /locus_tag="VYN16_001993"
     CDS             1415..2380
                     /locus_tag="VYN16_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1404467.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
BASE COUNT          924 a          375 c          480 g          735 t
ORIGIN      
        1 tttggggaaa ataaaatatt tgaaagcatt gataacttat ttgatattat agatggtgat
       61 aggggcaaaa attatcctaa atcagatgag ttgtttagtg aggagtactg tttattttta
      121 aatacaaaga atgttactaa aaacggattt tcattcgata caaagcaatt tatcactaaa
      181 acaaaggata aattacttcg aaaaggcaaa cttgagcgtt atgatatagt cttgacaata
      241 agaggtactg ttggaaatgt agcgtactac gatgaattaa taaaatataa acatttacgt
      301 ataaattcag gtatggtaat attacgtccc aagacaccaa atctaaatca gaaatttatt
      361 atccatgttt taaggaataa taattatagt cgagtgatat caggaagtgc tcagcctcag
      421 ttaccaatta caaaattaaa aaaaatactt ctccccctcc ccccactagc cctccaaaat
      481 gagttcgcag actttgtagt ccaggtcgac aaatcacaat tggcaatcca aaaatctctg
      541 gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg atattctgcc
      601 attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag atggtagtaa
      661 aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt aatattccaa
      721 gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt actcctgaag
      781 aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat ttagataaaa
      841 tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa tctatttgac
      901 agaaaagcaa attgaaaaaa taaatgcttt agcaattcaa cggtattctc caaatgagaa
      961 aattcaaaca gttagtcctt ctgccttaaa tatgattgtg aacttaccag aacaatttgt
     1021 ctttgggaag cctctttatc caacaatttt tgataaagca acgatactat ttgtccaatt
     1081 gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg ttttggtcaa
     1141 atttttacaa ttaaacggct atcgtttttc tataacggta gaagaagcag taaaaatgtg
     1201 tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact ccaaatggat
     1261 ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg atttgaatga
     1321 gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa ctgatattgt
     1381 ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta caattatcat
     1441 gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag agagtttaga
     1501 acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc tgttgcaact
     1561 atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt attaccagag
     1621 gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca caacagatga
     1681 tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg caaatttaga
     1741 caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg aatatatcat
     1801 taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga aggaaactta
     1861 tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag atttggcaat
     1921 aatagaccta ctagcatcga caggtatgcg tgtaggggag cttgtacagt tgaatcgttc
     1981 agatattgat tttgaaaaca gagagtgtgt tgtctttggt aaaggaaaga aggagagacc
     2041 agtatatttt gacgctcgta cgaaaattca tttaagaaac tatcttaacg acagaaaaga
     2101 tagtcaccct gctctttttg taacgttagt tggaaaagtc cagaggcttg gaattgctgg
     2161 tgtagagatt cgcttaagaa agttaggaga caaactcggt atacaaaagg ttcacccaca
     2221 taagttcaga agaactttag cgactaaggc aattgataaa ggtatgccta tcgaacaagt
     2281 ccaaaaactg ctaggtcata gcaagattga cacaaccctg gcctatgcca tggtcaatca
     2341 aaataatgtc aagcattcac accaaaaatt catctcttaa aagcaaatcc cgatattccg
     2401 aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg gctactttta tcaatggcta
     2461 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa taca
//
LOCUS       DAVLTM010000167         2486 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLTM010000167 DAVLTM010000000
VERSION     DAVLTM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.168"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..316)
                     /locus_tag="VYN16_001994"
     CDS             complement(<1..316)
                     /locus_tag="VYN16_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1404468.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAMKNAEDAKKAVEDAQKALDDAKAAQKKYADDQKKTEEKAKKEKEASKAEQAAN
                     LKY"
     gene            complement(602..880)
                     /locus_tag="VYN16_001995"
     CDS             complement(602..880)
                     /locus_tag="VYN16_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404469.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVASDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            complement(865..1104)
                     /locus_tag="VYN16_001996"
     CDS             complement(865..1104)
                     /locus_tag="VYN16_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404470.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(2240..>2486)
                     /locus_tag="VYN16_001997"
     CDS             complement(2240..>2486)
                     /locus_tag="VYN16_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404471.1"
                     /translation="LIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLGY
                     LLVIQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
BASE COUNT          830 a          474 c          318 g          864 t
ORIGIN      
        1 gatattttag atttgcagct tgttccgctt tagaagcttc tttctctttt ttcgctttct
       61 cctcagtttt cttttgatca tcggcatact ttttctgagc agcttttgcg tcatctaaag
      121 ccttttgagc atcttctact gcttttttcg catcctcagc atttttcatt gctgcatcat
      181 agtctttctc agctttagac tgactagcta cgggagcttc ttctgctctt acaaaagtag
      241 gctgagacgt aacaaaacca gcccctaaga tagcgacgct ggctagactt gttaaaatca
      301 tttttttctt attcatctaa atttacctct tttctgatag tcatttttag actatactta
      361 tattaagtat actccccccg taaatatttg tcaaatgttt tatattacat ttttattaca
      421 aatttctata tcataagctt ctactccaaa aagataaaga cactactttt gcccttttcg
      481 attctctttt taaacatagc atcatcttcc ttcattccta taaaaacatt tccgatgaga
      541 gagcttgttt tttaatttcg ttaaacaaca aaaaacgcaa gcttaagcct gcggacaatc
      601 tttatagcca tttcatttct ttcacatctg ttttacgaac tttagcatat ttcttagcgc
      661 ttgtaaaatg tagagtaact ggtttaaata ctccgctttt gtctcttcgt aaacctaaaa
      721 ctgcggatct tttattttta tcctcaaaaa atatgacgtc caatttcata gtatttttag
      781 ataaatcgct agctacgatg caacaactta ttattttaga atagaaaata tcaccgataa
      841 actcatataa agatatcctt tcgattacat cattgtacat aggatatttc taaaatcttc
      901 taaaataaaa acaccagctt gaatagcagg ttttgtttga ctagcataat ctcgtgtgat
      961 tttgtataag tcaaataaat gatgtaaatc agtcaaacaa aaattcactt tgaaaatctc
     1021 taaatactta taattcgttg atacttgaca tgtttttcca ttgaatcttt cttgatagtc
     1081 caaaatacaa tcaagtaatt tcaacgacaa actccatatt ttctcaaaat aaaaaagcac
     1141 ggggcagttc tcactattgc ccaaagtggt tgattagagt cggcttctct aactccctat
     1201 gttgtgctta ttaggcttat cgcaagggaa cagtttttag ctctgccaag ccacatagtg
     1261 tgcttactac tatgtgtcca tgtcaccatg aattgccgaa cggtttaacg agaaagtata
     1321 gtagattgaa acaagatgtg aacaaatcga ttaggaaagt caaattaatt tctagaaata
     1381 ttttaacagt cataacggac tattccagat tcaatctact atattacttt taggtttatc
     1441 ccaactcaat tatgacatct ttttttcaaa agtcaatata tctcactctt ttcaacgaca
     1501 agaaagaggc tgataatcga ccaacctctg tttttagata cagttcaagt ccaaactatt
     1561 ctttatagaa aagtccgata aattcgagca atagttaaca tacttacaaa tataactccc
     1621 aaaatagata acactatttt cacaaaactc agttccgaac ctatgacata tatatgaaaa
     1681 agaggggcta gtcctacaca tatatacaat aagtagccaa caagttttat ctcaccaatt
     1741 cctgcaaaaa aaatgtttca tataacgatt ctccatttac tccattaaat cagttttatt
     1801 ttagactatc ataaaacaat cacctcttca ataaaaacca ttggcgaaat actattctga
     1861 agcattgata gctcagattt ctcagaataa agctgtccta actatcgagc agctcaaata
     1921 catatatatg agcaattcta agcctagaat tgctacaaaa taaatgtttt caggctattc
     1981 taagtctttg actgcttaaa attttacatt ctgaaggatt ctaaacctag aatagctcaa
     2041 aatcacgaga actaaggtat tcgaaacttc agagtaatac aatccccata ggaattctcc
     2101 attcttatta agcatgatta aaatgtaatc ctaacaagtg atcctcgatt gtgtttgtag
     2161 cacttagtta cctagataag actatttccc tttatcatat ctgattttaa gccgttctaa
     2221 gatttaacct atgtttctat tacttgctat ttttaaataa ttcgtattcc ttttctttga
     2281 taaatggaac tgaaaaatta gaaaaaagaa gtaaaaccga aaacaaatag cctgcttttt
     2341 gaatcactaa aagatatcct aaaaataagt aaactataac atccaatgcg aaaagaaaaa
     2401 atattctcct cttcattaaa gtgcttgtgt caaataatct gatgaataaa aattttatcg
     2461 tggtatctac acaaattttt atcaaa
//
LOCUS       DAVLTM010000168         2469 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLTM010000168 DAVLTM010000000
VERSION     DAVLTM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.169"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..71)
                     /gene="ffs"
                     /locus_tag="VYN16_001998"
     ncRNA           complement(<1..71)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYN16_001998"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(104..571)
                     /locus_tag="VYN16_001999"
     CDS             complement(104..571)
                     /locus_tag="VYN16_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1404472.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(772..2058)
                     /locus_tag="VYN16_002000"
     CDS             complement(772..2058)
                     /locus_tag="VYN16_002000"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1404473.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(2289..>2469)
                     /locus_tag="VYN16_002001"
     CDS             complement(2289..>2469)
                     /locus_tag="VYN16_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1404474.1"
                     /translation="YGPTFGDNFDWEHVHFKFLIYYLVRYGIGCRKDFIVYHYRVAYR
                     LYLEKLVMNRGFISC"
BASE COUNT          811 a          609 c          351 g          698 t
ORIGIN      
        1 tcaaatcgct tagggctgct ggattcctcc cctgacccgc ttcacgcaga actgttgctc
       61 cactatttat tatatcacat tcctattcat tttaaaagca aaattatttt ttccgtctat
      121 ttctaaaaaa atcctgcatg atagctgcgc attcatcttc caaaattcct atttcaacct
      181 ccacacgatg attgagacgc tcatctgtca agatatcgta caaactccca gcagcgccaa
      241 atttctggtt tttagcccca tagaccacat ttggaatgcg ggcaagccca atcgccccac
      301 tacacatgac acaaggttca atggtcacaa aaagtgtgca atccagcaag cgccagctct
      361 cctcactcaa gttcgcatcc tctatagcca taatttccgc atgcataacc gctcgctgta
      421 attcctcacg cgcattatgc ccacgaccaa tgatttcccc atctttgaca atcacacaac
      481 caattggaat ttcatcgtgt tcaagagcaa tctcagcctc tctcaaagcc tccctcataa
      541 agacttcttt ttcttcaagc gtataataca tctcttttct cttttcctac ttatcgattt
      601 tattattata tcatgaatcc caacacaaaa gaagccaccg aatgcggtga ctctataggg
      661 agattattat gaaaaagaaa agtttaggat atttgttaca acaagttagg aggtcttctt
      721 gtaaccgttt atagtatacc cgacctatct taaacaaatc ttaaaaatct cttaggacca
      781 aacactttct aaaatatttg tttgttcacg accaggacct actgagaaag tagaaatacg
      841 aacgccaacc aattcactca cacgacgaac atagttacgc gcattctcag gaagatcttc
      901 caaattgcga actccggtaa tatcttctga ccaacctggc aactcttcat agataggctt
      961 gcaacgtttc aattgctcaa gactagctgg atagtagtca atacgttgac cgtcaagatc
     1021 ataggccaca cagattttca cagtatccaa accgctcaaa acatcaatag agttcaaaga
     1081 aaggttagta ataccagaaa cacgacggct atgacgcatc acaactgagt caaaccaacc
     1141 tacacgacgt ggacgaccag ttgttgtacc atactcatga cccacttcac ggatacgttc
     1201 tcccacttca tcaaacaact cagttgggaa aggaccatct cctacacgac tcgtataagc
     1261 tttacataca cctacaacct tgtcaatctt gcttggaccg acaccagaac caattgtcac
     1321 accaccagct acagggtttg atgacgtaac aaatggataa gtaccttggt cgatatctag
     1381 cataacacct tgtgcacctt caaaaagcac acgtttgcca ttatcaagcg catcattcaa
     1441 gataacagat gtatctatca cgtatttctt gatttgttga ccatattcgt aatattcttc
     1501 aaaaatatca tcgaaaacaa tcgctttact gtcatacaat ttttcaaaaa gacgattctt
     1561 ttcagcaagg ttacgttcta aacgctcgcg gaaaatatct ttatctaaaa gatctgcaat
     1621 acgaattcca acacgagcag ccttgtccat ataagctgga ccaattccct taattgtcgt
     1681 accaatctta ttgtcgccct tagcttcttc ttgcaagcga tccaactcga tatgataagg
     1741 caaaataaca tgcgcacgat cagaaatacg caagttatca gttgttacac cttcctcatg
     1801 aagatagctc aactctttta caagagattt aggatttaca accataccat tcccaatgac
     1861 agatattttt tcagggaaga aaatcccaga tgggatcaag tgcaacttaa atttcttacc
     1921 gtcaatcaca atcgtgtgac cagcattatc accaccttgg taacgtgcaa tcacttctgc
     1981 attcgctgaa aggaagtctg taatcttccc tttaccttca tcaccccatt gggtacctac
     2041 aacaacaact gaagtcataa tcttgtctga gccctcaggc tcttcctttc tcacatacat
     2101 ggcaggactc tcacctgcaa ttatatctta caatttatta taataaaaaa tcgccttttt
     2161 atcaagaaga aacaatagaa agatttgcta tttccaacta ttaaaaaatg atttagaaaa
     2221 attactagct atttactatc atctttccat aaaagagtaa attagttcgg aaatttacta
     2281 aaattacctc aacaagaaat aaaaccccga ttcattacca atttttcaag atacaaacga
     2341 taagcaacac gataatggta aacaataaaa tccttacgac aaccaatgcc atatcttact
     2401 aaataataaa ttaaaaattt aaaatgaaca tgttcccagt caaaattatc accaaatgta
     2461 ggaccatac
//
LOCUS       DAVLTM010000169         2432 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLTM010000169 DAVLTM010000000
VERSION     DAVLTM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.170"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            19..1596
                     /locus_tag="VYN16_002002"
     CDS             19..1596
                     /locus_tag="VYN16_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1404475.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            1656..>2432
                     /locus_tag="VYN16_002003"
     CDS             1656..>2432
                     /locus_tag="VYN16_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1404476.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERW"
BASE COUNT          870 a          331 c          481 g          750 t
ORIGIN      
        1 taaaatggag gtcttattat ggtaaagctt aataaggtat taaaagggag tgttgcattc
       61 ctgagtgcaa gtttattgct agcagcttgt ggcaataacg ctaattcaaa gcccgctgaa
      121 aaaacagctg atggtaaaac gaaaatttcg tttctatcta catggcaaat taatgatgtt
      181 cgtccgccta aagatgaaac acttgttggt caatggttag aagaacagct aggtattgat
      241 attgatcttg taactattcc tggcgaggga gtaagttcta aagtaaattc tcttattact
      301 agtaaccagt taccgactgt tttgttgaca acaggagata agagtgatat cgctggtatc
      361 aacaaacttg gtaaacaggg tgcattctta gatttgtcac aacacatgga caaactacca
      421 aactataaga agtaccttga aaaaaataat gcattagctc aaatagaaga tgatgaaaaa
      481 catatttatg ctttcacaaa attctttact gatgagaata tcatgtatac tacaccaatt
      541 ctgagaaaag acttacttgt tggttcagaa tttgaagatc tttcaaaaat taaaactgtt
      601 gatgactata caaaagtttt gaaatatttg actgaaaaac aaggttctcc agcatttatt
      661 caacgtaatg gctatgaagg cttcatgaag cgagttacac cattgtggaa tttaagccat
      721 cgtacatact atgactatga atcagattca tataaacatc cagttgaaca acctcaattg
      781 aaacaattcg ttgaatggtt gaaagaatta cgtaaagata acgtacttca ccctgactgg
      841 gcagttatga aagatgaaac ttgggaaggt ctattggctt ctaacaaggg ttcattcact
      901 gttgaccgta tgaatatcat tggagataat aactttgata aatcatttga ttggcagcca
      961 cttgtttatc cagaaattaa tggaaaacgt tatttgcaac cgaaaacacc gtacatttct
     1021 gattcaggtt gggtaatcaa tgccaatgca ccaaaagaag aaattgataa agcgttagaa
     1081 ttctttaatt tcttatacgc tgatgaaaat caagaaaaac ttgctattgg atttgaaggt
     1141 aaaacattta cacgtgaaaa ccctaatact caagcaggta ttaaatggtt gattcaaatt
     1201 tacggtgaaa atgctggaaa tgataaagca gaactgctgt ttaaacatgg taaccaagca
     1261 tttactcgcg ttcaaactaa attagatatt gaggcacagc caggaacatt ccctaaagtt
     1321 atgtatgatc aagtagataa aattaagaaa gatttaggtg gttttagacc agatactccg
     1381 gttctttcct ttacaactga agaattagat gttttaacta aatctgaagc aggattaaat
     1441 actttctttg atgaaaatat tattaaattc attgaaggac aaagagatat gagtgagtgg
     1501 gattcattta tcaatgaagc taaatctaaa gacttggata aagttacaaa agtatataat
     1561 gatgcctaca aacgtgtcaa agatagaatg aaatagacta aatggtagct ctctggtttt
     1621 tcagagagct actactgcct gagggggagt aagctttgta tgatgtagta attataggtg
     1681 caggaagttc gggcgcattg gctgcaattg cttcggggcg tctagggtta aaaacttgtc
     1741 taatagagaa gggaagtaga attggtggag ttcctattaa tacactaatg gggtcatttg
     1801 ctaatctata ttttgatgat tctggtagag taaatgcaag taaaattgtt ggggaattaa
     1861 tagaacgtat tatcaaaaaa ggtggtactg tctttaactc tatggaagaa ttgatgaatg
     1921 taaatgattt ttatcagatt acaattccct atcaaccaga agtttatgaa tcggttttaa
     1981 ctgatatgtt gattgagtct aaagttgata ttttactaaa tgttgaagtt aataatatta
     2041 tttatgatag taaaaatatt aaaagtcttg aaattttaag taatcaggaa gttaaattga
     2101 ttgattctaa agtttttatt gatgctacag ggaatgcaac aattgcttct gctagtggag
     2161 ctgatgttta tgaaaaaaat tcttcttacg gttgtttaat gagagttgga ggagttgata
     2221 tctcaaagac tatagaacat attgaaaaaa caaagcagtg gaactatata gaaggatata
     2281 atcagtggtt agaagaagta ttagcaaatc gaaagtacag agccactgga gcagttttaa
     2341 aagatccagt ttcttatgat catgccccta tgctttcaaa agacgaccat tttatgaatg
     2401 atgaaaaatg gaattacatt aaagaaagat gg
//
LOCUS       DAVLTM010000170         2431 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLTM010000170 DAVLTM010000000
VERSION     DAVLTM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.171"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(43..261)
                     /locus_tag="VYN16_002004"
                     /pseudo
     CDS             complement(43..261)
                     /locus_tag="VYN16_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            846..2197
                     /locus_tag="VYN16_002005"
     CDS             join(846..1287,1287..2197)
                     /locus_tag="VYN16_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSpn4 family transposase"
                     /protein_id="HEV1404477.1"
                     /translation="MKLSYEDKVQIYELRKQGYSLEKISNKFEINNSNLRYMIKLIDR
                     YGIEFIKKGKNRYYSPELKQEMIDKVLHENWSQDRVSLKYALPNRGMLPNWLAQYKKN
                     GYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYFKKVKRAGRQGRS
                     PRARKAEIVREMASGGFQLDLLLETARLARSTYYYQLKGLGGLDKDKDLKAEIQAIFT
                     EHKGNYGYRRVTLELRNRGFVVNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQ
                     GKAMLNQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKTFQSLNQLEQAIVDYIDYYNNKRIKIKLKGLSPVQYRTKSFG
                     "
     gene            complement(2297..2407)
                     /locus_tag="VYN16_002006"
     CDS             complement(2297..2407)
                     /locus_tag="VYN16_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404478.1"
                     /translation="MKLLLDMKNENRLIIIATHNPDIWNQADEVINLNQL"
BASE COUNT          833 a          424 c          455 g          719 t
ORIGIN      
        1 aagattccac gtttctaata attatactcg tggtgtcagt tactttattg acttgacaac
       61 ctgaagtaga tctctcgaat aaggttctcg aagagtaact tctactgctt tcccatcaaa
      121 gataccaaca cgtttaaatt caatgtagtc ttttttgaga tgtcctaagc ccataccaaa
      181 aggaaagtga tgaatcaatt ggatggtaca accaccttta tttcccattt gtttcaaatc
      241 atacaagttt actaccttca tgattaactc ctttatctag tttgtcgcat cgtttgagtc
      301 tggaaacgat aaactccgtg tctgttagaa aattctcaca cacgtcttgt gccagttgcc
      361 cttcacaggg aaatactctc agtccctact tacacaggca cgctaatcaa gacggagtga
      421 attcaatttt caaagaacag gtagctttat tatagataag agtagttgaa atttttatca
      481 catctttgag gttattggaa taatcaggta agtgctaatt tctagctctt ctcacaacac
      541 cgtacttacc ttttatgttt tatgaacagt agctatcatt tacattttca aactaccatt
      601 ctatcagact aaaagtcaaa atagacgctt ctacttcgag atatactcat ttaatggttc
      661 agcaattcgc tctacttctg ttacagaaat ttcatcactt tgacttctca tgatttgtta
      721 ttagttcact ttgagggctt gctcatgaag ccaaacagcg taagtcgtaa aagaaaaaag
      781 cagtacaaga tgaactgcac cccaaaagtt agacagaaaa aatctacttt ttggggtgtt
      841 ttattatgaa attgagttat gaggataaag ttcagatcta tgaactaaga aagcaaggat
      901 atagcttaga gaagatttca aataaatttg agataaacaa ttctaatctt aggtacatga
      961 ttaaattgat tgatcgttac ggaatagagt tcatcaaaaa agggaaaaat cgatactatt
     1021 ctccagaact aaaacaagaa atgattgata aagttttaca tgaaaactgg tctcaagata
     1081 gagtttctct caaatatgct ctcccaaatc gtggtatgct tccaaattgg ctggcgcaat
     1141 acaagaaaaa cgggtatact attgttgaga aaacaagaga gagagtacct aaaatgggac
     1201 gtaaacgtaa gaaaacttgg gaagaaatga cagaactaga gcgacttcaa gaggagaatg
     1261 aacgcttacg taccgaggtg gcttacttaa aaaagttaaa agagctggaa gacagggacg
     1321 aagccctaga gcgagaaagg cagagatagt tagagaaatg gcttcaggag gatttcaatt
     1381 agatttactt cttgaaacag cacgtttagc tcgctcgact tactactatc agttgaaggg
     1441 actaggtggg cttgacaaag ataaagatct taaagctgaa attcaagcta tttttactga
     1501 gcacaaagga aattatggct atcgtagagt tactcttgaa ctaagaaatc gtggttttgt
     1561 ggtgaatcat aagaaagttc aacgtctgat gaaagttctt ggtttaacag ctcgaattcg
     1621 tcggaaacga aagtattcct cctaccaagg agagattggc aagaaagcag agaatctcat
     1681 tcaacgccag ttcgaagcat cgagaccaat ggaaaagtgc tatacggatg tgacagagtt
     1741 tgccattcca gcaagcagtc aaaagctcta tttatcgcca gttttagatg gctttaacag
     1801 cgaaattatc gcctataatc tttcaacttc accaaactta gaacaaggga aagctatgtt
     1861 aaaccaggcc tttacagaga aacattatga gaataccatt ctccatagcg atcaaggctg
     1921 gcaataccaa cacgattctt atcatcggtt cctagagagt aagggaattc aagcatccat
     1981 gtcacgcaag ggaaacagcc cagacaatgg catgatggag tccttctttg gcattttgaa
     2041 atccgaaatg ttttatggtt atgagaagac atttcagtcg cttaatcaat tggaacaagc
     2101 tattgtagac tatattgatt attacaacaa caaacgaatt aagataaaac taaaaggact
     2161 tagtcctgtg caatacagaa ctaaatcctt cggataaatt aattgtctaa cttttggggg
     2221 tcagtacaag attgaactgc ttttacggat tcagataaaa taactttaaa atatagaaaa
     2281 tatatattgg ctgtaactat aattgattta aatttataac ttcatctgct tgattccaaa
     2341 tatcgggatt atgagttgca atgatgatta gacgattttc atttttcata tctaataata
     2401 atttcataat tccttgtgaa gtcgtcggat c
//
LOCUS       DAVLTM010000171         2398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLTM010000171 DAVLTM010000000
VERSION     DAVLTM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.172"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..810)
                     /locus_tag="VYN16_002007"
     CDS             complement(<1..810)
                     /locus_tag="VYN16_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV1404479.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDIL"
     gene            complement(797..1567)
                     /locus_tag="VYN16_002008"
     CDS             complement(797..1567)
                     /locus_tag="VYN16_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1404480.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(1593..2375)
                     /locus_tag="VYN16_002009"
     CDS             complement(1593..2375)
                     /locus_tag="VYN16_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV1404481.1"
                     /translation="MPVIRELIPKTYNRYFEPFVGGGALFFDLAPKDAVINDFNAELI
                     NCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRSADRDERIDMMSEVQRAARILYMLR
                     VNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELISAISVYINNNQLEIKVGDFEKAIVD
                     VRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFADQVRLRDAFKRLSDTGAYVMLSNSS
                     SALVEELYKDFNIHYVEATRTNGAKSSSRGKISEIIVTNYEK"
BASE COUNT          787 a          477 c          287 g          847 t
ORIGIN      
        1 taaaatatca aatgtttctc gtaaattatt cttagcctta taccaacctt gtccatcagt
       61 aatccacata aactcaacat taggaatagc tttagtttct tcagcaatca ttttgtaaga
      121 tctagccgtc tcatttaatt ttgaaccact tcctgaataa aaattaacct caatcagata
      181 aagcacttgt tcatttttta tgacaaaatc aaaacgtttc acagtattcc catcattcgt
      241 aatagcagat aaatcgacag aaaaaatttc ctctatttca ttttgttcaa tctctttaaa
      301 taaattctct cctaaaatat acccttcagc ttcaagatag ctttgaacaa tattttccat
      361 cgcatctcca gtccgatttt tgcgcccatt agtatccata ccaacctcaa cacctgtaac
      421 gtagtcaact aaatttgata ctaaatgatt ttgcaataaa tcaaatatac ctgatttttc
      481 caaaaacata gtatattctt caatactata gtttcgttta ctaaaatcaa aatagaaatc
      541 ttttatcgga tccttaacaa taatcgtatc tctcaaacgt ttagcaataa gtagaggcac
      601 aactttcaaa atttctggat aattttgata taagtctaaa aaatcctctt gtatattttt
      661 actaccaata agactattca taatatttag agcaactttt atactagata catttttata
      721 aaccttagga aaatctgtat aataagtcca gtcagctact gtgtcggtca tcgttgacaa
      781 ccactcatca aaatttctag tttgtttcat tttctgcctc caacctcttc cttgcaattt
      841 ttaaatattc tttttcagca tcgataccta taaatcttct acctaaccgc ttcgcaacaa
      901 cacccgtagt gccactacca acaaatgggt ctagaatata gtcaccctct ttagtagagg
      961 ctaaaataat acgttctaac aaatactctg gtttttgagt tggatgtttc ccagcccatt
     1021 tttcaacttt ctttgttaaa gaaccggtcc agacatcttt catttgtttt ccatcattca
     1081 attctttcat taaatcataa ttgtagtaat gacgagcttt tttatcattt tttctggccc
     1141 ataaaatggt ttcagtagaa tgggtaaaat aacgacaaga taaattgggg gcagggtttg
     1201 ttttctgcca agtaatatta ttcagaattt taaaaccttc ttgttctaat gccattccaa
     1261 ctgagtatat gttgtgcaaa ctacctgaaa tccataccgt cccattaggc ttcagaactt
     1321 cttttgctag gcgaatccat ttacgattaa actcatgttt ttcttcgaat gaagaaattt
     1381 tatcccaatc tcctttatca acagaaacta cttgtccccc agaattagat attccaccat
     1441 tacttaaaaa ataaggtgga tcagcaaaaa tcatatccat actttctggt ttcatttttg
     1501 ataagaactt gaacgtatct gaatgaacaa gaatcatctt atttttattg tagtatggtt
     1561 ttgtcataag aacacctcca tacttatatt cgttattttt cataatttgt gactataatt
     1621 tcagaaattt ttcctcgact tgaagatttt gctccattag ttcgggtagc ttcaacataa
     1681 tgtatattaa aatccttata caactcctct actaaagcac tagaagaatt tgataacata
     1741 acataagctc ctgtatcact caatctctta aaggcatctc ttaatcttac ttgatctgca
     1801 aaagagaatc cctcatgagt ataagacgta aatgcactcg tctcagacaa tggaatatat
     1861 ggagggtcaa aatacacaaa atctcctgtt cgaacatcta caattgcctt ttcaaaatct
     1921 cccactttaa tttctagttg attgttattt atataaactg aaatagcaga tatcaattcc
     1981 tcatcaacaa ttttaggatt cttataacgt ccatatggaa cattaaattg attcttagaa
     2041 ttcacacgat atagaccatt aaagttcact ctcaacatat atagaatacg tgcagctctt
     2101 tgtacttcgg acatcatatc tattctttca tcacgatctg cagaacgtaa atctaaataa
     2161 tattcttttg aattatattc ctgatgaact ttcaaaattt caatcaattc ttgaggattg
     2221 tccttaattt gttgatagca atttattagt tcagcgttaa aatcattaat aactgcatct
     2281 ttaggagcca aatcaaaaaa taaagctcca cctccaacaa aaggttcgaa atacctgtta
     2341 taggttttag gtattaattc tctaataaca ggcaataatt gtcttttacc acctgtcc
//
LOCUS       DAVLTM010000172         2371 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLTM010000172 DAVLTM010000000
VERSION     DAVLTM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.173"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(1..480)
                     /locus_tag="VYN16_002010"
                     /pseudo
     CDS             complement(1..480)
                     /locus_tag="VYN16_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(726..1037)
                     /locus_tag="VYN16_002011"
     CDS             complement(726..1037)
                     /locus_tag="VYN16_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404482.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1052..2338)
                     /locus_tag="VYN16_002012"
     CDS             complement(1052..2338)
                     /locus_tag="VYN16_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1404483.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          688 a          540 c          428 g          715 t
ORIGIN      
        1 acgaatagtt gctgtttgtg ccgcatcatc ataagaaacc acttcagcga caaacttgta
       61 ctcaggaatt ttacgacgag caccaaacaa ctgctcattt tcagatggtg taccatagta
      121 aaatcctgta gccagttcac gttgggcaac cttccacatc tcgtccacca agtcttgttt
      181 gatagcttca aacttttcag gactttcaag ataggcatcc acagccgcct tgtagcagtt
      241 ggttactgtt aatacatagt gaatagactc catacgtcct tcgattttta gactgtccac
      301 gccattttca atcatatctg gaatgtggtc aatcatagac atgtcaacgg ctgacattga
      361 aaattcttct ggaatctcac cctgcaaact cttacgttct ttcccaaatg gcatatcgta
      421 aaggtcgtac ttccaacggc atgactgaga acatccacca cggttggcat cacgcatact
      481 ctagacggat tgttttgaca attctaatct tttagacttt gcataataac tgttaaacag
      541 ttgaaaattg acccagtttt aacaagtagt caaaatacca gataaaggaa aaaagtcttt
      601 aaaatcttaa aaacgcatag tatcaggtat tgaacaatct tgatattatg cgttttattg
      661 tgggaagatt tacttcattt tctcttggaa ttgagttttc tcctacttca aaactagatg
      721 atttgctatt taaaatagcg ttccttatga ttttggcaac ctggattaaa gcgagaaaaa
      781 caaaaggggc aacttaagct ttctttatat tgcttatatg ttagtagttt tagacaaaca
      841 ccacataaaa taggcttatc ctgtatcaaa gataagggat acggctcaaa agggtgattt
      901 tctaatgaat catgacaccg ataacaagca taatactttt tacaatcata gcattgtaaa
      961 gcaataatat ccagcttgct atggtaatga atgcatcttg tttgcttatc cactaagaaa
     1021 ccctgtgcct ctatcatttc cttttccttt cttaagctcg aactgtgacg ctggttccat
     1081 cttccttata aagattgatg agtccttctt ttaatgcacg aaccatgtct cctgattgaa
     1141 cgggttgagg caccttaata gtcaatagtt ccattggatt tggagcgcgg tcgattttat
     1201 tgcctttggc atcatgcaaa tcttcaatat aggtttcaaa atgacggaaa cctggaccat
     1261 aaaactcaac ttggtcccct tcgttaatga catttcgttg acgaatagtt gctgtttgtg
     1321 ccgcatcatc ataagaaacc acttcagcga caaacttgta ctcagggatt ttacggcgag
     1381 caccaaacaa ctgctcattt tcagatggta taccgtagta gaaacctgtt gccaattcac
     1441 gttgggcaac cttccacatc tcgtccacta agtcttgttt gatagcttca aacttttcag
     1501 aactttcaag ataggcatcc acagccgcct tgtagcagtt ggttactgtt gatacgtagt
     1561 gaatagactt catacgtcct tcgattttta gactgtccac accattttca atcatatctg
     1621 gaatgtggtc aatcatagac atgtcaacgg ctgacattga aaattcttct ggaatctcac
     1681 cctgcaaact cttacgttct ttcccaaatg gcatatcgta aaggtcgtat ttccaacggc
     1741 atgactgaga acatccacca cggttggcat cacgcatact catgtggttt gaaagagtac
     1801 aacgtccaga gtatgaaata cacatagctc catggacaaa ggcttcaatt tcaacatctg
     1861 tacgtttgcg gatctcagct aattcttcca ttgaaacctc acgcgctaaa acgacacgag
     1921 tcaagcctag ctctttccag aactcaaggg tttcatagtt agtggcactg gcttgggtag
     1981 aaaggtggat ttcaaggcct ggtgcttcag tcgctgcaat cataatcaag gctgggtcag
     2041 atacgataac tgctgcaatc ccgatatcac gcagtttacg gaaccactca ccagcaccag
     2101 cttcatttcc ttcgtgcata accatattag ccgctacata gaccttggca ccatacttgg
     2161 ccgcaaactg cacgccttct tccatctgtt cgaaagtaaa gtttcccgca cggctacgaa
     2221 gaccataggc ctgaccaccg ataaagacag catctgctcc atactgaaca gctaccttta
     2281 gcttctctaa agtccctgca ggtgataaaa cctcaggacg ttttaatgtt tttgtcataa
     2341 tttctcctat ttgcaatata aaagtttgac a
//
LOCUS       DAVLTM010000173         2298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLTM010000173 DAVLTM010000000
VERSION     DAVLTM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.174"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..649
                     /locus_tag="VYN16_002013"
     CDS             <1..649
                     /locus_tag="VYN16_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404484.1"
                     /translation="SRLIKPLEGEVLLDNKSINSYKEKDLVKHIAILPQSPIIPESIT
                     VADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIA
                     LALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADY
                     LFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
     gene            1037..1426
                     /locus_tag="VYN16_002014"
     CDS             1037..1426
                     /locus_tag="VYN16_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1404485.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            1392..1705
                     /locus_tag="VYN16_002015"
                     /pseudo
     CDS             1392..1705
                     /locus_tag="VYN16_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          958 a          316 c          390 g          634 t
ORIGIN      
        1 gtctcgactt ataaagccat tagagggaga agtattgctt gataataaat caattaattc
       61 ttataaagaa aaagatttag taaaacacat agctatatta cctcaatctc caataatccc
      121 tgaatcaata acagtagctg atcttgtaag ccgtggtcgt ttcccctaca gaaagccttt
      181 taagagtctt ggaaaagatg accttgaaat aataaacaga tcaatggtta aggccaatgt
      241 tgaagatcta gcaaataacc tagttgaaga actttctggg ggtcaaaggc aaagagtatg
      301 gatagctcta gccctagccc aagatacaag tatcctactt ttagatgagc caactactta
      361 cttggatatc tcatatcaaa tagaactatt agacctcttg actgatctaa accaaaaata
      421 taagacaacc atttgcatga ttttgcacga tataaatcta acagcaagat acgctgatta
      481 cctatttgca attaaagaag gtaaacttgt tgcagaggga aagcctgaag atatactaaa
      541 tgataaacta gttaaagata tctttaatct tgaagcaaaa attatacgtg accctatttc
      601 caattcgcct ctaatgattc ctattggcaa gcaccatgtt aactcttaat aattgaatat
      661 tggaattcat ttaaaagtcg caaattttga actgaccctc aaaagttagg ccaaaaaact
      721 aactaaagga ggtcagtttt ttaatggcaa atacagcaca gaatttaaga taaaattagt
      781 aaaagaatat catgaatgaa aaataggata tagagaataa cagcaatatt aaaaagatca
      841 ggactgataa taaacaaaaa gaaagtacaa cgactagttc aaaagctaaa acttcaagta
      901 aaaagttatt caagaaaatc tagaaaatac tcatcctaca aaggacaggt aggaaaaata
      961 tcagacaaca aaataaaaag aaatatttat taccgaaaaa aaggggaaat cgagtcaagt
     1021 accaacaggc tcatctattc caaaaagata tttactcgat ggaaaaactc ctagaattag
     1081 tgctacaaat attgacaatg gtatcttagg ttactacgaa gatatagatg ataaaaatta
     1141 tagagttttt gaaaatttta tatctgtttc atttttagga gcagtctttt atcataaata
     1201 taaagcaagc ctagatatga aaattcattg tttaaaatta aaaaataaag aattgaataa
     1261 agaagtagca ttctatttaa cttcaattat taggcaagct ttaaaaaata ctgaatataa
     1321 agatcaaata tctagcacag tattgccaga tatcaaaatt aaattaccga tagacagtag
     1381 aggtactcca gattggaatt acatggaaag atatagagat agataaagag agagggagag
     1441 atagataaaa agtccacccc cattttttta tggagaattc aaaaatgtaa ggttaagcaa
     1501 agaagaatat aaaaatctaa aagaaaaatt aaactcacac acagatataa tgataaataa
     1561 actatccaga tacatggaaa gcagtggtaa gacctatcaa aaccactatg tgacaatctt
     1621 aaaatggtat gaagaagata aagacaaact aagacagaaa ggtttaaata aaaaaatgaa
     1681 ttatgatgta ggagaatctt tatagataaa aagaggtgga aaggcactat taaagacttt
     1741 aagtctaaaa aggtatcaaa gtatgccaga gataaaaaaa tataaatatg acactttaaa
     1801 ggtgattaga aaattaaaga aatgctagaa gacttttaga tgacagttac caagagggtc
     1861 taattttcac tgctacttat gcagtgggaa ttggaccttt ttatataagc aaattctatt
     1921 tcaaaattgg ttaccaagaa agacttcttt tcactgttaa gtataagagg gtaatttata
     1981 gaattttgtt acattaagat aaatattttc tgatggtcaa attttaaaaa gattaataat
     2041 tctcgataaa ttttgaacct taacaattga atagaccaag acaagacgtt aattattttt
     2101 ggagcgtaat aacctatccg tcaagatctt tcggctgaat aattcggcaa aaaaagttct
     2161 gctaagtaaa aagaaagtga gcgataagta agagtttgaa tatagggagg gataccctat
     2221 ccgctatgaa gaaaatgagg ataatgatac ttctaaggtt gaagccggct catccggaat
     2281 agaggcggag gtgagatt
//
LOCUS       DAVLTM010000174         2190 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLTM010000174 DAVLTM010000000
VERSION     DAVLTM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.175"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..627
                     /locus_tag="VYN16_002016"
     CDS             <1..627
                     /locus_tag="VYN16_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1404486.1"
                     /translation="ITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQI
                     MNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILNKLLSYSEDL
                     KHHYQLLLFHFQNKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIINALQLPYSN
                     AKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(761..848)
                     /locus_tag="VYN16_002017"
     tRNA            complement(761..848)
                     /locus_tag="VYN16_002017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Ser,seq:gct)
     gene            complement(855..928)
                     /locus_tag="VYN16_002018"
     tRNA            complement(855..928)
                     /locus_tag="VYN16_002018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(892..894),aa:Ile,seq:gat)
     gene            complement(964..1034)
                     /locus_tag="VYN16_002019"
     tRNA            complement(964..1034)
                     /locus_tag="VYN16_002019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1000..1002),aa:Gly,seq:tcc)
     gene            complement(1054..1126)
                     /locus_tag="VYN16_002020"
     tRNA            complement(1054..1126)
                     /locus_tag="VYN16_002020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1091..1093),aa:Phe,seq:gaa)
     gene            complement(1130..1203)
                     /locus_tag="VYN16_002021"
     tRNA            complement(1130..1203)
                     /locus_tag="VYN16_002021"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1167..1169),aa:Met,seq:cat)
     gene            complement(1217..1306)
                     /locus_tag="VYN16_002022"
     tRNA            complement(1217..1306)
                     /locus_tag="VYN16_002022"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1268..1270),aa:Ser,seq:tga)
     gene            complement(1317..1390)
                     /locus_tag="VYN16_002023"
     tRNA            complement(1317..1390)
                     /locus_tag="VYN16_002023"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Ile,seq:cat)
     gene            complement(1404..1477)
                     /locus_tag="VYN16_002024"
     tRNA            complement(1404..1477)
                     /locus_tag="VYN16_002024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Met,seq:cat)
     gene            complement(1483..1556)
                     /locus_tag="VYN16_002025"
     tRNA            complement(1483..1556)
                     /locus_tag="VYN16_002025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1520..1522),aa:Pro,seq:tgg)
     gene            complement(1572..1645)
                     /locus_tag="VYN16_002026"
     tRNA            complement(1572..1645)
                     /locus_tag="VYN16_002026"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1609..1611),aa:Arg,seq:acg)
     gene            complement(1655..1740)
                     /locus_tag="VYN16_002027"
     tRNA            complement(1655..1740)
                     /locus_tag="VYN16_002027"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Leu,seq:taa)
     gene            complement(1748..1819)
                     /locus_tag="VYN16_002028"
     tRNA            complement(1748..1819)
                     /locus_tag="VYN16_002028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1785..1787),aa:Gly,seq:gcc)
     gene            complement(1842..1914)
                     /locus_tag="VYN16_002029"
     tRNA            complement(1842..1914)
                     /locus_tag="VYN16_002029"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1879..1881),aa:Thr,seq:tgt)
     gene            complement(1933..2014)
                     /locus_tag="VYN16_002030"
     tRNA            complement(1933..2014)
                     /locus_tag="VYN16_002030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1978..1980),aa:Leu,seq:tag)
     gene            complement(2020..2092)
                     /locus_tag="VYN16_002031"
     tRNA            complement(2020..2092)
                     /locus_tag="VYN16_002031"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Lys,seq:ttt)
     gene            complement(2117..2189)
                     /locus_tag="VYN16_002032"
     tRNA            complement(2117..2189)
                     /locus_tag="VYN16_002032"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Asp,seq:gtc)
BASE COUNT          644 a          563 c          435 g          548 t
ORIGIN      
        1 attactatgg atatgtttag tccttactat gacttagcta gacaactttt cccatgtgct
       61 aaaatcgtgt tggatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg
      121 cgtgttcaaa tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgc
      181 tactggaagc tcatacaaca ggatagtcgt aaactgagtg ataagcgatt ttatcgtcct
      241 acttttcgta tgcacttaac caataaagag attttaaaca agcttttgag ttattcggaa
      301 gacttgaaac accactatca gctcttactt tttcacttcc agaataagga accagagaaa
      361 tttttcgaac ttattgagga caatcttaag caggttcatc ctatttttca gactgtcttt
      421 aaaaccttcc tcaaagataa agaaaagatt attaacgccc ttcaactacc ctattctaat
      481 gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc ctttggattt
      541 cggaactttg aaaacttcaa aaaacggatt ttcatcgctt tgaacatcaa aaaagaaagg
      601 acaaaatttg tcctttctcg agcttagctt ttcttcaacc cactacagtt gacaaagagc
      661 cataaaaata acttcagatt agtaattcaa ttaagtttta cggatctgaa gctttattgt
      721 ctctaaaaat aaaaaagaga gttacaaact ctcattaaaa cggagaataa gggattcgaa
      781 cccttgcgcc agttacccga cctaacgatt tagcaaaccg tcctcttcag cctcttgagt
      841 aattctccaa ttaatgggca cgagtggact cgaaccaccg acctcacgct tatcaggcgt
      901 gcgctctaac cacctgagct acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt
      961 caaagcgggt gacgagaatc gaactcgcga caacagcttg gaaggctgta gttttaccac
     1021 taaactacac ccgcataaat actatcaata aaatggcgcg agacggaatc gaaccgccga
     1081 cacatggagc ttcaatccat tgctctacca actgagctac cgagccttat tgcgggagca
     1141 ggatttgaac ctacgacctt cgggttatga gcccgacgag ctaccgagct gctccatccc
     1201 gcgttaataa tataaaagga ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg
     1261 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccaata ttcaaatgga
     1321 ccttgtagga cttgaaccta cgaccactcg gttatgagcc gagagctcta accagctgag
     1381 ctaaaggtcc aacaagatca ttatagcggc gaaggggatc gaacccccga cctcccgggt
     1441 atgaaccgga cgctctagcc agctgagcta caccgccatg aatcgggaag acaggattcg
     1501 aacctgcgac accttggtcc caaaccaagt actctaccaa gctgagctac ttcccgagtt
     1561 aaatagaaaa atgcacccta gaggagtcga acctctaacc gcctgattcg tagtcaggta
     1621 ctctatccag ttgagctaag ggtgctccat attatgccga ggaccggaat cgaaccggta
     1681 cgatcgttac caatcgcagg attttaagtc ctgtgcgtct gccagttccg ccaccccggc
     1741 ctctctaagc gaacgacggg attcgaaccc gcgaccccca ccttggcaag gtggtgttct
     1801 accactgaac tacgttcgca ctgttttctt ctatctaaaa atgccggcta catgacttga
     1861 acacgcgacc ctctgattac aaatcagatg ctctaccaac tgagctaagc cggctcattt
     1921 gttatatctt aatgcgggtt aagggacttg aacccccacg ccgttaagcg ccagatccta
     1981 aatctggtgc gtctgccaat tccgccaaac ccgcatatat gacccgtact gggctcgaac
     2041 cagtgaccca ttgattaaaa gtcaattgct ctaccaactg agctaacgag tctaaaataa
     2101 cttgcgttac cttaaacggt cccgacggga atcgaacccg cgatcttcgc cgtgacaggg
     2161 cgacgtgata accgctacac tacgggacct
//
LOCUS       DAVLTM010000175         2184 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLTM010000175 DAVLTM010000000
VERSION     DAVLTM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.176"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(66..182)
                     /locus_tag="VYN16_002033"
                     /pseudo
     CDS             complement(66..182)
                     /locus_tag="VYN16_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(195..2033)
                     /locus_tag="VYN16_002034"
     CDS             complement(195..2033)
                     /locus_tag="VYN16_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1404487.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(2051..>2184)
                     /locus_tag="VYN16_002035"
     CDS             complement(2051..>2184)
                     /locus_tag="VYN16_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1404488.1"
                     /translation="YPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT          734 a          453 c          300 g          697 t
ORIGIN      
        1 taaaaatcaa tacctaacaa ttttaagtta tcttctgtag gattttctgt tgcttctcct
       61 aatccacctt tgggtaacac atcctgaact gataatccct taccatcttc attatatgct
      121 ccctctactt gattagctgc aaccgctcca ccccaaagaa aatcatctgg aaaaatggtc
      181 ataactttcc tccattataa tattaccagt aattccttag aatgctcgat tgtctgatta
      241 ttaggtaata ctaatacatc tagaaaatca ttggtattcg ttacaattac tggtgtaact
      301 gtttcgtagc ctttagtctt gattaaattc aagtccattt caaaaatcaa ctgatttttg
      361 aaaactctgt ctccttcttc tacatgacta ataaaacctt gaccttttag ctcaacagta
      421 tctaatccaa tatgaattag taactcaaca ccctcatcac tcttcaatcc aattgcgtgc
      481 ttagtcggaa aaatatttgt aattttccca tcaaatggtg cataaacctt accttcactt
      541 gggataatcg ctactccgtc tccaattagt ttatctgaaa atgttttatc ctggacatcg
      601 cttaacggaa tgatttctcc tgatatagga gaaaatatca tttttttatt tgaaactcca
      661 gcttcaactt ctaaattgct agaactctct tcttcatcga ttccaaatat ataagctaat
      721 acaaaggtaa taacaaccga aatgaccgcc acaattaaag catttacaat atttgatggc
      781 acatcagaat aaataaattg aggcaacgct atcaaagatg ggacagcaaa tagatatgct
      841 ttaacactag taagacctgc aaataatccc gctaatccac caccaatcat agctgcataa
      901 agcggttttt tatattttaa agtcacacca tataatgcag gttcggtaat ccctgcaagt
      961 aaggctgaga aacctgctgc aaaagcaatt tgttttgtat tattattttt actctttaat
     1021 gcaacagcca tcgaagcagc cccttgagct aagtttgacc ctaacattgc tggaagaatt
     1081 aatacgtctg gagtagcaat agatgccgcc aaaaaaatag gtgcaaaagc ccaatgcatt
     1141 ccagtcataa caataaatgg cataatagca ccaagaatag ctaatgtaag ccatccagct
     1201 acaccataca tttgcccaac tagatttgat aatccttcac caacaattac tccaataggt
     1261 ccgactacaa ctaaggcaat acagcttgat actaataata ctagcgtagg ttgcaaaaaa
     1321 ctcttagtaa tagctggtgt taatttagca attatttttt caatatattt catcaaccaa
     1381 accataataa gaattggaac gactgatgaa ccataactag ctggtgtcac aggtgcacca
     1441 aataaactaa gaggattccc tgattgcacc atttgaacaa aatttggatg gagaagtaca
     1501 cctgctacag acatagctaa tgtagatgtt acttttaatt tttgtgatgc agaataagct
     1561 aataacagcg gtaagaaata atatggagca tccccaaaaa atgtcaaaaa agcaatagtc
     1621 tgagaatctg attgcaatat accaagcatt ggtaaaatga ttaccaagac tttcaacata
     1681 cctcccccta acattgctgg aatgattgga gtcatggaac cagcgatata ctcaatgatt
     1741 ctttctaaaa tattcccttt gtgcccttga acaactgaat cggattcaaa attgccaagt
     1801 ttaacgaatt ctttataata attagctaca tcattaccaa gtataatttg atattgtcca
     1861 ttctttttca taatacctat tacacctggt atcttcttca catcatcatc attgactaaa
     1921 ttttcatctt ttaattctaa tcttaaacgt gttacacaat gggtaactct attgacattt
     1981 ttttcacctc caattacatc gaggattttt tgtaccgtat ctttataact catggtattc
     2041 tcctattcta ttaatctaaa ttttttgtta agcgacgaat atgagccatc aaataaacta
     2101 attcactaga agtcagcaaa taattgtact ccgtttgtat aaacattgcc acctgttcac
     2161 cacattcata ttctctagga tatt
//
LOCUS       DAVLTM010000176         2140 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLTM010000176 DAVLTM010000000
VERSION     DAVLTM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.177"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..559
                     /locus_tag="VYN16_002036"
     CDS             <1..559
                     /locus_tag="VYN16_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1404489.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VYN16_002037"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VYN16_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1404490.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="VYN16_002038"
     CDS             1424..2029
                     /locus_tag="VYN16_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1404491.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2140)
                     /locus_tag="VYN16_002039"
     CDS             complement(1977..>2140)
                     /locus_tag="VYN16_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1404492.1"
                     /translation="ISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDLL
                     FQKVSQLFE"
BASE COUNT          740 a          306 c          372 g          722 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg tcgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacacta agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa
//
LOCUS       DAVLTM010000177         2111 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLTM010000177 DAVLTM010000000
VERSION     DAVLTM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.178"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            98..1591
                     /locus_tag="VYN16_002040"
     CDS             98..1591
                     /locus_tag="VYN16_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1404493.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1604..>2111
                     /locus_tag="VYN16_002041"
     CDS             1604..>2111
                     /locus_tag="VYN16_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1404494.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNEL"
BASE COUNT          737 a          314 c          412 g          648 t
ORIGIN      
        1 ttcaggtgac gcatagatat agttaattga agctttgttt gaaatctgat aaaataatga
       61 tattactaag ttttaaaaac taaagaaaag ggaagatatg attacaggcg aattaaaaaa
      121 taaaatcgac cagctgtggg aaattctttg gacagaagga aacgcaaatc ctttaacaaa
      181 tattgaacag ttgacttatc tcttatttat gaaagatttg gatagtgtcg agcttggacg
      241 tgaaagtgat gctgaatttc tagggattcc ttatgaggga gtttttccaa aagataaacc
      301 tgaataccgt tggtcaactt ttaaaaatat aggagatgct caggaagttt atcgtttaat
      361 gactcaggag atttttccgt ttattaaaaa tctcaagggg gatacagatg atacagcctt
      421 ttcacgatat atgcgagaag ctatttttca aataaataaa cctgctacgc ttcaaaaggc
      481 aatttctatc ttagatgttt ttccaactag gggattagat gtagattttg ataatgacaa
      541 acaaagtatt actgatatcg gagatatcta tgaatatctg ttatcaaaat tgtcgaccgc
      601 aggtaaaaat ggacagttcc gtacacctcg tcacatcatc gatatgatgg ttgagttgat
      661 gcaaccgact atcaaagata tcatctcaga tcccgctatg ggttctgctg gcttcttagt
      721 atctgctagc cgttacttaa agcgtaagaa agatgaatgg gaaaccaata cagataatat
      781 caatcatttt cataatcaga tgtttcatgg aaatgatacg gatacgacta tgttgagact
      841 tggggcgatg aacatgatgc tacatggagt agaaaatcca caaatcagtt accttgactc
      901 gctgtctcaa gataatgaag aagccgataa atatactttg gttttagcaa atcctccttt
      961 taagggctca cttgactaca attcaacctc taatgacctt cttgcaaccg taaaaaccaa
     1021 aaaaacagaa ttactctttc tttctctttt cttgcgaact ttaaaaccag gtggacgagc
     1081 agcagttatc gtacctgatg gtgtcctttt tggttcgtct aaagctcata aaggaattcg
     1141 tcaggaaatt gtagagaatc ataagcttga tgctgtaatc tcaatgccta gtggtgtgtt
     1201 caagccttat gctggagttt caactgctat tctcatcttt acaaaaactg gtaatggtgg
     1261 tactgacaaa gtctggtttt acgatatgaa agcggatggt ttaagtttgg atgataagcg
     1321 acaaccgatt agcgacaatg atattccaga tattatcgaa cgctttcatc atcttgaaaa
     1381 agaagcagaa cgtcagagaa cggatcaatc tttctttgtt ccagttgctg agataaagga
     1441 aaatgattat gatttgtcta tcaataaata taaagagatt gagtatgaaa aagttgagta
     1501 tgaaccaaca gaagtcatat taaagaaaat caatgattta gaaaaagaaa ttcaagctgg
     1561 cttggctgaa ttggaaaaat tactcaagta gggaggtggc tgtatgaaaa aagtgaagtt
     1621 gggggaagtt tgtgagattt tatctggtta cgcttttaaa tcatcacaat ttaatgataa
     1681 caaaattggt ctccccttaa tacgaattcg agatgtagaa agaggttttt ctgataccta
     1741 ctttgaagga acttatcccg aagaatatct tattaaaaat ggagacttat tgattacaat
     1801 ggatggcagt tttatattga aaaaatggga gggggattta gcgctactta atcaacgtgt
     1861 ctgtaaaatt aaaattacgg ataaaagtgt agatgaaggt tatatatctt ggttgattcc
     1921 taaattttta aaagaaattg aagataagac cccttttgtt acagtgaaac atctttcagt
     1981 agctaaaata aaagatatct catttgtatt gcctaataaa ctagaacaaa agctaattgc
     2041 aaaaaaacta aatacgataa gtcaaattta tgattttcgt aaaattcaat cagaaaaatt
     2101 taacgagctc g
//
LOCUS       DAVLTM010000178         2084 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLTM010000178 DAVLTM010000000
VERSION     DAVLTM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.179"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            73..1098
                     /locus_tag="VYN16_002042"
     CDS             73..1098
                     /locus_tag="VYN16_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1404495.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGLSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIRIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1088..>2084
                     /locus_tag="VYN16_002043"
     CDS             1088..>2084
                     /locus_tag="VYN16_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1404496.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRG"
BASE COUNT          729 a          295 c          374 g          686 t
ORIGIN      
        1 ttgtaactgc atctattgac aatgataatt attatcgata taatagactt gaaatatgtt
       61 caaggagttt ttatgaaaaa caaatttttt ctaatagcta ttttagctat gtgtatagtt
      121 tttagcgctt gttcttctaa ttctgttaaa aatgaagaaa atacttctaa agagcatgcg
      181 cctgataaaa tagttttaga tcatgctttc ggtcaaacta tattagataa aaaacctgaa
      241 agagttgcaa ctattgcttg gggaaatcat gatgtagcat tagctttagg aatagttcct
      301 gttggacttt caaaagcaaa ttacggtgta agtgctgata aaggagtttt accatggaca
      361 gaagaaaaaa tcaaagaact aaatggtaaa gctaacctat ttgacgattt ggatggactt
      421 aactttgaag caatatcaaa ttctaaacca gatgttatct tagcaggtta ttctggtata
      481 actaaagaag attatgacac tctatcaaaa attgctcctg tagcagcata caaatctaaa
      541 ccttggcaaa ctttatggag agatatgatt agaattgatt caaaagcctt aggtatggaa
      601 aaagaaggtg atgagttaat caaaaatact gaagctcgta tatccaaaga attagaaaaa
      661 catccagaaa tcaaaggaaa aatcaaagga aaaaaagtat tatttactat gattaatgct
      721 gcagatacat caaaattctg gatttatact agcaaagatc caagagcaaa ttatttaaca
      781 gatttaggtc tagttttccc tgaatcatta aaagaatttg agagtgaaga tagttttgca
      841 aaggaaattt ctgcagaaga agcaaataag ataaatgatg ctgatgtaat cataacttat
      901 ggtgatgata aaactcttga agctttacaa aaagatcctc ttttaggtaa aataaatgca
      961 attaaaaatg gtgccgttgc tgtaattcca gataatacac cgttagcagc ctcatgcact
     1021 ccaacaccac tttcaataaa ctatactatt gaagaatatc taaatctttt aggaaatgca
     1081 tgcaaaaatg cgaaataaaa aacaaataaa cctaggcata atttttgtaa tctgcctagg
     1141 tcttcttatt acaatatttt tgtcattaaa gcttggaaca aaagaaatta atatcagaga
     1201 ttttttagca gcttttggaa tgggtaatac aaatgatgat tttattaaat caattatata
     1261 taaaagaata cctagaacta tttttgcaat tttagcaggt tctagtcttg ccataagcgg
     1321 tgtattgatg caatcagtta ctagaaaccc aatagctgat ccaggtatac tcggtataaa
     1381 cacaggagca agtcttagtg tagtaattgg tctttctttt ttaggaattt catcaagcat
     1441 aagccatata agttttgcaa tcattggtgg cttagtaagt gcaatttttg tatacgcgat
     1501 tgctgtaagc ggaaaagcag gccttacccc tataaaactt gccttatcag gaacttgtgt
     1561 tagtatggct ttaagcagtt ttgtaagttt tttaatttta ccgaataata acgtcttaga
     1621 caaatttaga ttttggcaaa taggtagcct tggagcagct acattatctt ctatatctac
     1681 actactacct tttataattt taggtcactt gatagctata tttatttcat cagatttaaa
     1741 cgctttagct atgggtgatg aaatggctgt tggtcttgga gttaatgtta ataggataag
     1801 atcacttgca ataattgcaa gtgtgctttt atgttcaagt attactgcaa ttggtggacc
     1861 tattggcttc gtaggtctta tagttcctca cttttgtggc ttatttataa gcaaagatat
     1921 acgcacaatg accatttctt catcttttat aggtgcagag ctcttgctta tatgtgatat
     1981 aatcggccgt atgttaggta aaccaggtga aattgaagta gggataatta ctgcaataat
     2041 cgggggtcca gtacttattt atgtaactat gaaaaataga gggg
//
LOCUS       DAVLTM010000179         2021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLTM010000179 DAVLTM010000000
VERSION     DAVLTM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.180"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            16..822
                     /locus_tag="VYN16_002044"
     CDS             16..822
                     /locus_tag="VYN16_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1404497.1"
                     /translation="MTSLSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSV
                     LYAALSTIVSVAVCSMYAYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGL
                     LNTAWALVLPSAVSTYYIILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVI
                     FMIIALYCIVGSWNSFTPPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYY
                     QQIAAQGGGIGMRIAIKMGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            836..>2021
                     /locus_tag="VYN16_002045"
     CDS             836..>2021
                     /locus_tag="VYN16_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404498.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHIGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEI"
BASE COUNT          666 a          277 c          388 g          690 t
ORIGIN      
        1 ttatggtggg tagtgatgac gtcactttca gaaccagttt ggctatctaa aaacgttgtt
       61 aaattatttc ccttaaaaat agatattact gcatataagt ttatttttgg ggattctaaa
      121 ctatggcaag catatggtaa tagtgtattg tatgctgcac tatctacaat tgttagtgtt
      181 gcggtatgta gtatgtatgc ctatccactt acaattcctg aaattagggg taaaaaattc
      241 tggaatactc ttattttgat tccaatgttc tttagtggag gaatgatccc gctttatata
      301 caagttgcaa acctcggttt attgaatact gcttgggctc ttgttttacc tagtgctgtt
      361 tctacatatt atataatttt gtttagaaca cagtttaaat caatcccaat ggagcttcgt
      421 gagtctgcta gaattgatgg ggcaagtgaa tttagaattt tttggaattt agtattacca
      481 ttatcaaaag ttattttcat gattatagca ctttattgca tagttggttc atggaatagt
      541 tttactcctc cattacttta tttgaatgac gacactaaga tgcctttatc tatttatttg
      601 agaaatttag ttgttacaaa tacaactgat actgctgaat tatcactttc tgcaggtaat
      661 acatattacc aacaaattgc tgctcaaggt ggaggtattg gtatgcgtat tgctattaag
      721 atgggtacta ttgttgttag tgttattcca attatgatga tttatccatt tattcagaaa
      781 cattttgtta agggaatcct gctaggttct gtgaaaggtt aataaggtaa ttattttgca
      841 aaatttaaaa aaatatcttt tattagatag cggtttagta gttgggtata cattagaaaa
      901 tgctcaatta aaattaaata agctaaaaaa agatactgaa aacaatccac tatttggtga
      961 agagtatttt gccgaaaatc ctaaattatg ggaagttcgt tttgataatt catatccaaa
     1021 cgttatctat gataaagaat tgaaattata tagatgttgg tatcaaacat ttgttagtga
     1081 tgaagcaagc gaagagactc ctttagcaga acgtggagag aaggaataca ttgtcaagtc
     1141 atctagaatg acagcacttt gctatgctga aagtaaagat ggagtaaaat gggaaaaacc
     1201 aaatctcaat ctagtaaaat ttaaaggttc aaaagataat aatattgttc tgatgcatgc
     1261 acacggtgca ggtatcatgc tagatgaaca ggagttggat cctaagaaac gatataaatt
     1321 agtttcaaaa ttagaatttt ctccgaatct tcattacatg gttgttgttt tttcagaaga
     1381 cggtattcat tttgacgaac ctattccatg gaaaaaatgg aatcctgcag cagatagtca
     1441 taattttcca tttagagatc caaaaactgg taaatacgct gttattacaa gaacctgggc
     1501 aaataacgta cgtctaagtg caattacttt cagtgatgat tttattaatt ggtcagaacc
     1561 taaagaaatc cttaggggag atggatttga agatcaaatt tattctatgc cagtaatttc
     1621 atacgcggat atctatttag gttttgcttc aatgtaccac gaaggcgaca ctttaaatga
     1681 aaactatgat aaagtagact tagaattgaa attctctgct aatttggaac aatgggatag
     1741 tgtatgtaag ggcgactatc tcattgaaag gggaggtgga gtatacccgc agaatgctga
     1801 ttttgatgct agttgtattt tcacggctgc tccaataatt gaagatgata agatttggtt
     1861 ctactatatg ggtggaaatg gtaatcacat tggcttccga gaaagttcat tagctagagg
     1921 atatattaag aaagaccaat ttgcttatta tactaataag agagaaggaa ttccagctaa
     1981 aatatctaca gttagcttcc aaacttacga tgatgaaatt t
//
LOCUS       DAVLTM010000180         2017 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLTM010000180 DAVLTM010000000
VERSION     DAVLTM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.181"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(330..755)
                     /locus_tag="VYN16_002046"
     CDS             complement(330..755)
                     /locus_tag="VYN16_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404499.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKV"
     gene            complement(884..1243)
                     /locus_tag="VYN16_002047"
     CDS             complement(884..1243)
                     /locus_tag="VYN16_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404500.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMSLG"
     gene            complement(1289..1495)
                     /locus_tag="VYN16_002048"
     CDS             complement(1289..1495)
                     /locus_tag="VYN16_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404501.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(1482..1643)
                     /locus_tag="VYN16_002049"
     CDS             complement(1482..1643)
                     /locus_tag="VYN16_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1404502.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT          636 a          420 c          234 g          727 t
ORIGIN      
        1 aaaaactact cgctatcccc tctcacatta ccctacatat cgtttgacat gcgactgata
       61 gttcaggaaa acttcaagga gaacttttct ctcatccact atgcaggact tactatttca
      121 cttctactcc tataggctca atttgacact tttctttttc gaattgctca tatgcctcct
      181 gaatagtgag ttcgatttat ccaaatttat ttttgtctat tttgatgaat acttccacga
      241 agatagtctg aaaatgaatt tgcaccaatt ttccttctct ttttaagcat attattccag
      301 atatgattgc cctcttgttc agtaataaat tacactttaa acgcttcaat cagtatatct
      361 cctgttgtca tatgttctaa agaaaattct tctacatatg atttaacatc tcttaggtta
      421 ttacttccta atatcccatt atgctttttc gctaaggaaa tagaagccgc ttctccctta
      481 ccaataatct tgttactatc atgatttctt gttaaatctc tatataatgc gtattcttca
      541 gttccaatgt ctatgctcac aatctcagct gaacccttag ctaccaactg gtctatccta
      601 gattttaaat ggggaattgt aggtatattg atttcatcat acacctcttg tggaataaca
      661 attttacccg aatagagctt ttctaaaaga tgttcagtgc caacccataa aaatactgaa
      721 atacaatctg catcaataaa tacacgacta gtcaaacgac aactcccccc tcttcatcta
      781 gcccatatac aatatcatat ctgaaagcat ctagtaacag ttcctcatac ttcccttgcg
      841 aaattctgtt attttctaaa agttgttcag ttgaattaat atattatcct aatgacattt
      901 cctttttact ttctgacaaa ggacgatata aacttgtatc atagcctaat cttgaagctg
      961 tctctataac actaatatcc atatttttaa tttcttctgc atcaaggtat ccatcattcc
     1021 tcaatctata taacataact ttatgactga taccataaaa ctgacccaat tttataatat
     1081 cttctacttc aagatgagtt ctattggcat tttctctgat ttcctcaacc atcctataca
     1141 gtgaagatgg gaaaattaaa aaataagaag caaactgatc cgcttttctt tcagtttcat
     1201 ctccttcacc aatcaagata agactgactg aactcttctt cacctcatca taataaagat
     1261 gatacagtcc atgtgctaaa gaaaatcttt accttcctaa tggcatatct gaattgactg
     1321 caatgagact gaactgagtt cctttataac agactccgct aatattcttt ccgagtccat
     1381 aaaataccag cgtcaaattt tctatctttt gtaccaattt aaaaatatct atcggcgatt
     1441 caccatcagc tcccaacttt tttctaagat ttggagcttt attacttaaa tccattcgtg
     1501 atattccttt cacctttttc cattttggca atcatgcttt gattcaaaga caaatactca
     1561 gcaatcttat cttgagtaag atttgaagat attctaagtt cttttattct ttttccaaca
     1621 tcacatacat ttatcatatt catacacctc tcataaaaag aatagcacac tcttgtcata
     1681 attttttaaa taaaaaaatt atgacaaaac aaggaagcaa tttattgatg ctgcttaaaa
     1741 atctaaaatt gatgagttcc tatcaaaaat cgtatcttca acctcaaaac agtacttaaa
     1801 gctatccgac tcggtttaca ttgtcaaatt tagattttat ttgagcataa ctttctagtt
     1861 tgctttttga tttttgttta atatagtagc aaaaattgaa ggaaatatct ccacaagaaa
     1921 acgcatacta ttaagctttt tcaagaccta ataatatgcg ctgttctgat ttgaaagata
     1981 ttccattatt attttactgt aatcaagcca tctggct
//
LOCUS       DAVLTM010000181         2007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLTM010000181 DAVLTM010000000
VERSION     DAVLTM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.182"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..378
                     /locus_tag="VYN16_002050"
     CDS             <1..378
                     /locus_tag="VYN16_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV1404503.1"
                     /translation="GQEKVIRSAILEYELDRQKGHVLIKALIRGDEFSHKLKSLEDEL
                     CGVLELELEKKVLRPSVAEYHFYYIKPKRLVLQSHNQRQEIDSLDIDLGYGVNYNPIK
                     CPHILVSGGTGRGKSVFISFLIA"
     gene            536..925
                     /locus_tag="VYN16_002051"
     CDS             536..925
                     /locus_tag="VYN16_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404504.1"
                     /translation="MQTFNYDDFKNEKGIVSFSEAEQIYSSLLNSSNQLDKEFQEEWT
                     TFVLLCVEYASARGKWLTLSREEKLANDESRTVTHNKVIYQLKILKGLASEQGNDVAW
                     FEQFNDDRKRIGDFACYVAYIYALNAR"
     gene            1092..1865
                     /locus_tag="VYN16_002052"
     CDS             1092..1865
                     /locus_tag="VYN16_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1404505.1"
                     /translation="MKTLLIGDLHLKSQLILPIVDKIIQTHNIKRIIFLGDYVDLHGQ
                     TNNIQLYAKDLTFLYSWKIDKEQNGIDVINLIGNHDAYYLLGEQAPFSIQNLEVFFAI
                     QQLLQDLNLQVAYQLDDYLVSHAGFNLIFDPKEWHFNLYTKEHEEELDILAYTIGPMR
                     GGKALGGSPLWAHFRELELLPNQDFPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDN
                     KYQFIGNGDLLVYDQGHFEVISTDWATDKILKQLPQPQI"
BASE COUNT          704 a          323 c          345 g          635 t
ORIGIN      
        1 ggacaagaaa aagttattag aagtgcaatc ttagaatatg aattagatag acagaaaggt
       61 catgtactta tcaaggctct tataagaggg gacgagttta gccacaaact taaatctctt
      121 gaagatgaac tttgtggcgt gcttgaacta gagcttgaga aaaaggttct tcggcctagt
      181 gttgcagagt atcatttcta ttacataaaa ccaaaaaggt tagttctaca atctcacaac
      241 caaagacaag agatagatag ccttgatata gatttaggat atggtgtaaa ttataaccca
      301 atcaaatgcc cacatatttt agtatcagga gggacaggga gaggaaagtc tgtcttcata
      361 tctttcctga tcgcctagtg cttctgttca agagatggcg aacaacaaaa cttgtcctgt
      421 tactcttaaa gatttaaatg tagggcaatt caatggaaac ttgtggttcg cttgttctga
      481 tgttttacct gtaacaaaat agctaagatt agataatata taaaggagtt ttacaatgca
      541 aacttttaac tacgatgatt ttaagaatga aaaggggatt gtttcatttt ctgaagcaga
      601 acaaatctat tctagtctcc ttaattcctc caatcaactt gataaagaat ttcaagaaga
      661 atggacaaca tttgtacttc tatgcgtaga gtatgcctct gcacgtggta aatggctcac
      721 actctcaaga gaagagaaac ttgccaatga tgaatctaga actgttactc acaataaagt
      781 tatttatcaa ctaaagattt taaaaggttt agcaagtgaa cagggcaatg atgttgcttg
      841 gtttgaacaa tttaacgatg acagaaaacg tattggcgac tttgcttgct atgtagctta
      901 tatctatgcc ttaaatgctc gatagacaat tagaggatat tatggctata tattactcta
      961 tttctgatat tcatggaaat tattaaataa catcttaaga ataattaatt ctttttatct
     1021 atcaccgaaa caatgtgcta taacttaatt cgattcttag actaatatca aaaataaaga
     1081 ggggaatata tatgaagacg ttattaattg gtgatttaca cttaaaatct caactgattt
     1141 tacccattgt tgacaaaatt atccaaacac ataatattaa gagaatcatt tttcttggtg
     1201 actacgttga tttacatggt caaactaaca atatccaact ttatgccaaa gacctcactt
     1261 ttttatactc ttggaaaata gataaagagc aaaatggaat agatgtaatt aatcttatag
     1321 gtaatcatga tgcttattat ttactgggag aacaagctcc tttctcaatt caaaatctag
     1381 aggtcttttt cgcaattcaa caattattac aagatttgaa tttacaagta gcttatcaac
     1441 tagatgatta tttagttagc catgctggtt tcaatctgat atttgaccca aaagaatggc
     1501 attttaacct ctataccaaa gagcatgaag aagaattaga tattctagct tatacaatag
     1561 gaccaatgcg tggtgggaaa gctcttgggg gttctccttt atgggcacat tttagagaat
     1621 tagaactgct tcccaaccaa gactttccta aacaaattgt tggacatacc cctaaagaat
     1681 ctattgatat ttctaaaaat gttattggta tagatacttt ttcattatat attgacaaag
     1741 ataataaata tcaatttatt ggtaacggag atttgctcgt ttatgaccaa ggacattttg
     1801 aggtcatttc aacagactgg gcaacagaca aaatattaaa acaattacca caaccacaaa
     1861 tttaatagct acttctcatc atgtacctaa gcaaaactgc cgacaagcag tcagcaagct
     1921 ggaagtgtgt ttagcggagc ggtgcttagg atcacatgat atatgacctc atataataaa
     1981 aaaatagtcc gcacttcttc ctatatt
//
LOCUS       DAVLTM010000182         2003 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLTM010000182 DAVLTM010000000
VERSION     DAVLTM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.183"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(1..1015)
                     /locus_tag="VYN16_002053"
                     /pseudo
     CDS             complement(1..1015)
                     /locus_tag="VYN16_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            complement(1028..1777)
                     /locus_tag="VYN16_002054"
     CDS             complement(1028..1777)
                     /locus_tag="VYN16_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1404506.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            complement(1789..2003)
                     /locus_tag="VYN16_002055"
                     /pseudo
     CDS             complement(1789..2003)
                     /locus_tag="VYN16_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          695 a          369 c          216 g          723 t
ORIGIN      
        1 atatactaaa atttttatac cgacaatcaa tagttcatta attatactta aaatagctga
       61 ctctttgtaa ttatctgaat tatatttcat ccccaattta tatattccta ctatctttgg
      121 ctttcgttcc aatatttgtt taactatgaa ctgttttcta tttgtgtttg aaatatcaat
      181 cgcttctatc actggggcat ttatttctat aaattctttt tttaattgtt tagtatcttt
      241 gggaagacaa tatcctccaa atccaaaaga aggattatta taaaattttc caattcttgg
      301 atctaaacaa acacctttta ttacaacttc agcatttaaa cttctcctct cagcaaaaga
      361 atctagttca ttaaaaaaag caacacggag agctaagaat gtgttagaaa aaagcttaat
      421 tgcttctgct tcagtaggag aaactaacat aacattttta atattggcag tactatgagt
      481 actaatcgaa aggaacaact ctgcaatttt tcttccttca actgtctcat ctccaacaac
      541 tatgcgactt ggatataaat tatcatatat agaacaacct tctctcaaaa attcagggac
      601 aaaaatgata ttttttgtat caaacagcct ttttaatttg tttgaaaagc cgaacggaac
      661 tgttaacttt aaaataatct ttccattagg ttttaccctc agaatcttcg ataccgtttg
      721 ttcgatttca tatgtattaa aactaccaat tttctcatca taatctgtcg gaagcgcaat
      781 aatataataa tcaatattat ttttaatttc agaaaatgta tcaaaaaaag taatatttaa
      841 gttattctcg caaaaaaact tcataagctc ttcattttta gatggaagaa tgcccttttt
      901 ttaaattatt tatttttaca gaatctatat catatgcaac aactttatat ttagatgcaa
      961 atagtaacgc gtaggccagc ccaacatgcc ccaaaccaat tactgctata ttcataaaac
     1021 tacttcctta tttcttaatc caaaatctaa tagaataagc tgccccattc cttaaataca
     1081 actctttaat attgtttaaa agtttttcaa ctgatttcca gattatcaaa atctgagatt
     1141 tatagcacaa tattgatgat attctatcaa tataatgttt ttcatcaagt tcctcttgat
     1201 acatttttaa ttctttagtt tttcccatat aactaaccat actactatca cttacacatg
     1261 ggaagtcctc ataatatatt actttataac gcataaattc aagcgccctt ccaatactat
     1321 tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg acaatatacg acacatttgt
     1381 cgtctaaatg cattaacagc tcttttatga tatcattctt taatgtgtcc tcatttttta
     1441 attcactata gatatgacgg tatagaaaat tgccatttct atctttccta tagagacatt
     1501 catagtacga taagtgtcta aaatcacatt gtagacgttc acaagctaac ctgtcttctt
     1561 tcttcctttc ttcaatcgga tatttcccaa ggttacacaa cttatgaaat tgcttagcag
     1621 agggctgtag ctgttggctc aaagggtaac cagaaaatat agtaataaca agtacaattt
     1681 ctccttctga agttaatttt gaaatataat caccacagga aaaaattgcg tcatctaaat
     1741 gtggagataa aaagatatac ttagtattgt tactcataac cattccctct acaatttatc
     1801 taaaaactca ctaagtgtct gattaaattc cacatcatca aaaaaattca ccttattctt
     1861 aataatgaat atttcgttaa ataaacatat atataaatat ttcaatatcc tttcaatatc
     1921 ctttcaatat catcctctaa attctcctca atattttgta tcagcccatt tacaatctta
     1981 ttaaaaaaga taagctcttt atc
//
LOCUS       DAVLTM010000183         1976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLTM010000183 DAVLTM010000000
VERSION     DAVLTM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.184"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(98..1261)
                     /gene="ltrA"
                     /locus_tag="VYN16_002056"
                     /pseudo
     CDS             complement(98..1261)
                     /gene="ltrA"
                     /locus_tag="VYN16_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          508 a          477 c          336 g          655 t
ORIGIN      
        1 attgaaacta gaataatata cctctacttc taaaatattg ttagaaatcg atttgacttc
       61 cctgatcgat tcgtcctatt cttatttcat tttactataa ttgatagtgg tcgccccagc
      121 cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc cataatcgtc
      181 tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc aagcgctcat
      241 ctatgctagc gactatgcgc ttcatatttc ccagcgagaa atagtttatc catcctcgaa
      301 tagacaaatt cagttgctca atacgtcttg ttaagtctat actccatttc ctctgtgtta
      361 gtttcttcaa tttaagctta aatctccgaa cactatcttg atggggacga cttttccaac
      421 catctgatga tttccagaac ccaaaaccta agtatttcag ctctcttggt ctggtaatct
      481 tagccttggt catatttact ttcaaaccta gtcgcttctc aataaaacga ctggctgaat
      541 acatcacacg cttagcggct gcctcgcttc cgaccgtaat cacacaatca tctgcgtagc
      601 gcacaaatcg aagtcccctc ttttctaatt ccttgtccaa ttcattaagc atgacattgg
      661 ataagagagg agataaattt cctccctgtg gcgtgccaac tagcgtttta tgacgctgac
      721 cattaatgat aacacctgaa tgaagatact tacgaatcag ggattccgta tctccgtctt
      781 cgataatgtt atgtaccaag gacatcaatc tatcttgagg aaccgtatcg aaaaatttct
      841 ctaggtctat atccactatc cactcatagc cgtcatttaa gtactctaag aacttcatga
      901 tggctttttc acatgaccta tttggtctga agccataact cgtatctgag aaatggggtt
      961 cacaaatggg gctaataact tggacaatgg cctgttgaat cattctatcc ataactgttg
     1021 gaattcctag ttgacggata cctccgtttg gtttagtaat ctcaactcta agaactggtt
     1081 gaggcttata ttttctctgt tttatcagtt ccttagtcaa gcgccagttt tgtctgagat
     1141 aattatccat ctcttcgata gtcattccat caatcccagc tgagccttta ttggatttta
     1201 cttgattgta ggcttccagc atattttcgc gtgataatat cttatctagc aattttgaca
     1261 tgtgcgtatt cctttcttgt tttggtgcct gagggacatc ttatattggt gaaccttcat
     1321 ctagtcatta cgtgtcaaag tccagttcta tcagaccact cattttacag acacaagtga
     1381 agtaggatta cttcgatctg tcgttttctc tttctagcga cagacctcaa acagtctctc
     1441 cccagactgt ttgagtattc accccttcgc tccacttcca ttacggaagt ttcctcacta
     1501 ctatgggctc ggctgacttc tcatgattcg ttgttactac gctttcagcg ctcatgaaac
     1561 ctcacgggat aagtcgtaca tctttcctcg tttatacaag atatgaggac gttaaaaggt
     1621 tacagtgaaa ataggaaatc tgacgcagag tctttagact ctagaaagat ttatcttttt
     1681 cacacaaacc ttagtccgaa ttcaattact ctcgtgattt acacatatag gttacgacta
     1741 cctttttgga ctttagagtt tgaagcctcc ttatccgcta tatacgcctt gctatcacgt
     1801 tcctgttcgt agagccacga tttcgctatc tcttcctctg acgattctta tttcctgcgt
     1861 cagacttaaa acggtctatc cccagaccgt tttaatccgc tacctcacga tagtcaggct
     1921 tgggaatcgc tattgggttc accggtagca ggtgcccacc gaggacttac acctca
//
LOCUS       DAVLTM010000184         1878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLTM010000184 DAVLTM010000000
VERSION     DAVLTM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.185"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..913
                     /locus_tag="VYN16_002057"
     CDS             <1..913
                     /locus_tag="VYN16_002057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1404507.1"
                     /translation="LTEMYALPKYYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGT
                     MLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHLISTTSDVMTILV
                     VSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLFIAVGMLMPDMRP
                     LFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGDLVSFTRLMALGL
                     SGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFF
                     GKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            929..1405
                     /locus_tag="VYN16_002058"
     CDS             929..1405
                     /locus_tag="VYN16_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV1404508.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1439..>1878
                     /locus_tag="VYN16_002059"
     CDS             1439..>1878
                     /locus_tag="VYN16_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404509.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYL"
BASE COUNT          589 a          252 c          382 g          655 t
ORIGIN      
        1 attgacagaa atgtatgctc tgcccaaata ttatgagaaa gatcctacac ctgtattagc
       61 accattttac tttacatttt ttggaatgat ggttgctgat ttaggctatg gtttactatt
      121 gtttttagga acaatgttag cattaaaaat ttttcatcta ccttcagcaa ctaagagatt
      181 tttaaaattc tttaatatat taggggtagc cgttgcaatt tggggtggaa tctatggctc
      241 attttttgga tatgagttgc catttcatct gatatctaca acctctgatg ttatgactat
      301 attagtagtg tcagttgtgt ttgggtttat tacagtattt gcaggtttgt tagcttcagg
      361 actacaaaaa gtaagaatga agaaatatgc agaagcatat aattcaggat ttgcgtggtg
      421 tgttattctg cttggcttgt tatttattgc tgttggaatg ttgatgcctg atatgagacc
      481 gttatttgta ttagggaaat gggtatctat ttttaatgct gtggggattt tgattgtttc
      541 tattattcaa accaaaagct tgtcaggtat tggagcagga ttgtttaatc tatataacat
      601 ttcatcttat ataggtgatt tagttagttt cactcgattg atggcattag gattatctgg
      661 agcaagtata gcatcagctt tcaatttaat tgttggtttg tttccgggaa tattggctaa
      721 actgacaatt ggattagtat tattcattct tttacatgcg atcaatattt ttctatcgtt
      781 actatcagga tatgttcatg gagcacgtct gatatttgtt gaattttttg gtaagtttta
      841 tgagggtgga ggaaaaccat ttcaaccttt gaaggcttct gagaaatata ttaaggttat
      901 tacaaagaat taatggagga tatatataat ggaacattta gcaacttatt tttcaaccta
      961 tggaggagct ttcttcgctg cattgggaat tgtattggcg gttggattaa gcggtatggg
     1021 gtctgcttat ggagttggta aggctgggca atctgccgca gctttactga aagaacagcc
     1081 tgaaaagttt gcctcagctt tgatattgca attattgccc ggaacacaag gattatatgg
     1141 ttttgttatt ggaattttaa tttggttgca attaactcca gaacttcctt tagaaaaagg
     1201 cgttgcttat ttctttgtag ctcttccaat tgctattgta ggatactttt cagctaagca
     1261 tcaaggaaat gtagcagtag cgggaatgca aatcttggct aaaagaccaa aagaattcat
     1321 gaagggagca attttagctg ccatggtaga aacctatgca attcttgctt ttgtcgtatc
     1381 attcattttg acccttcgtg tataagaaat aaatttgcaa ttcaaaggag gtgtctaaat
     1441 gagcaattta gaaaacttac gagagtctgt tattgaacaa gctcatgaaa aagggcgtat
     1501 gaaattattg gattccaaaa agaagattga tgatgaattt gaaatgcaaa agtcgctcat
     1561 tataaagaaa aaagaagctg aacatgaacg aaagttaaaa gaattgcaac agaaatatca
     1621 aataattttt caacaattaa aaaataagga acgccaatca acgttagtat caaaacagaa
     1681 aatattaaaa gaactttttc aatctgcttt actagaaatg gaatcttgga gtgcagataa
     1741 agaaatggag ttcatctatc gaattctgga acgatattca caacaagagg tcatagtaac
     1801 ctttggggaa cggactttag ctaaattcaa tttggaacaa ttagagaaat tgaaattctc
     1861 ttttccaaat tatttatt
//
LOCUS       DAVLTM010000185         1867 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLTM010000185 DAVLTM010000000
VERSION     DAVLTM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.186"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(104..1240)
                     /gene="lctO"
                     /locus_tag="VYN16_002060"
     CDS             complement(104..1240)
                     /gene="lctO"
                     /locus_tag="VYN16_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1404510.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT          563 a          413 c          330 g          561 t
ORIGIN      
        1 atttgtccta ttcttatttc atttcactat agttttcgat agcaatttat tcttccaata
       61 cacgaagaaa aacctccaca ttcagtggag gcaatctgtt ttatcaatac aattttaagt
      121 cacgagggtc aactgggaag gttgggttgt atggattgtg acggagctta aagtgtttga
      181 catcttcaat ggtctgagct ccagacaatt gcataactgt cttcaattcc gcattcaagt
      241 gttcaaagac ttgacgcaca ccgacactac caccgagagc taagccataa ataacagggc
      301 gaccaatagc taccaagtct gctcctgaag ccaaggcttt aaagacgtgt tgaccacgac
      361 gaacaccaga gtcaaagaca atcggcacac gtctatcaac tgcttctgcc acttcttgaa
      421 gcgagtcaaa ggcagctggt ccaccgtcga tttgacgacc accgtggttg gttacccaga
      481 taccagaagc tcctgcagca agtgaacgtt caacgtcctc acggcattgt ggtcccttga
      541 catacacagg aagaccagag tattcagcga taaattctac atcgcgtgga gacaagcgtt
      601 gtttagctga tttgtaaaca aagtccattg atttaccagc accttctggc aggtattctt
      661 caacaatcgg catgccaact gggaagacaa aaccattacg cttatccact tcacgattcc
      721 cccctacagt agcatctgcc gtcaagacaa tcgctttata accttcagcc ttcacacggt
      781 ccatgatgtg gcggttgata ccgtcatcct tactaaagta aaattggaac caatggggtg
      841 tcccttgaag ggcttcagaa atctctggaa ggtcaacagt agagtaagag ctggttgtat
      901 aaagagaacc aaactcatgc acaccacgcg cagtcgccac ttccccctgt tcatttgcca
      961 atttatgagc cgcaacaggc gccataatga ttggtgaaga tagtttttca cctgcaaatt
     1021 caatctctgt acttggattt tcaacatcgc aaagtgtatg aggaacgatg agtttgtggt
     1081 taaaggcgcg gatattctct cttaaagtga aagtatcttc cgccccacta gcgatatagc
     1141 caaatgctgc tttaggaata acttgttgcg ccattggctc caaatcatag gtattgatga
     1201 agtctacatg accttctgca ttgcttgttt tatatgacat aaaatgccct ccttgattaa
     1261 gtaagcgttt gctttttact acacaactat aataactctt ttttcaatca ttttcaagag
     1321 ttttgcttaa aaaccatctt attttttcat acaatatact agccctactt tccttaaact
     1381 ctaaacctta gtctatatta agtgaatcgt tgagattttt tgctgccagg catttgaact
     1441 acttttttat ttgcggattc tatctactaa tactataaaa aaaacaagtg aataagacac
     1501 cagaacatcc tctaatagga cctatggact tatatcagtc ccatatatac tgtttaagca
     1561 agatactatc ctagttgacg ctaacttcct atcccactta ttaatttttt atttatagtg
     1621 aatctgaaca aatcaattgg ggaatccaag tcaatttcta gtactttttg aacagtttca
     1681 gcgtactatc gtaaaatgaa ataaaacatg cacaaattga ttagggaatt taaaccaatt
     1741 tctaacaatg tcttagaaat caaagtgtac tatttcaact tcaatatact acacttcatt
     1801 ttctccttaa attcagtctc tttttgaatc aaaaaatcct ctggtaagag ccagagggta
     1861 agaattt
//
LOCUS       DAVLTM010000186         1794 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLTM010000186 DAVLTM010000000
VERSION     DAVLTM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.187"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..464)
                     /locus_tag="VYN16_002061"
     CDS             complement(<1..464)
                     /locus_tag="VYN16_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404511.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIG"
     gene            complement(464..1228)
                     /locus_tag="VYN16_002062"
     CDS             complement(464..1228)
                     /locus_tag="VYN16_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404512.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1324..1727)
                     /locus_tag="VYN16_002063"
                     /pseudo
     CDS             complement(1324..1727)
                     /locus_tag="VYN16_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          699 a          352 c          263 g          480 t
ORIGIN      
        1 cctataaaga aactagcatt gtttgcaata cttataccat aaaattctct taaaaaatca
       61 acctccttta tctccaaaga gaagctaaaa ccattactaa atgcaatcag aaaaatcaat
      121 aaaaataaag tcgccgtcca aatccccgta ctaagagctg ctaatttgaa actaaaactg
      181 gtaaagtgct taattgattt cagacgaatg cgacactcca acctattaaa atagttattc
      241 atcaaataaa aaaagaacaa tatatatgtg aacggaaagc agtatactcc agtcgtcata
      301 tcttgaagta agactaagat ccattctaat acatttggat ggattgaata ttggcgacag
      361 cgcaataaat atactgtact agataaaaca caggatagca gtaatataaa ataaaccaat
      421 actgctaaga aatctttttt ataaaattga acaaattgtt tcattataca tagtcctctg
      481 aatgtagaaa aaatgtacca taaacaacca aacaactaac aaataaaata aaagcaagat
      541 gcccactaac taaggaaaga ctgatatctt tctgatatcc caaagctaat gttgtcacag
      601 gttctaagta agatagccct aaaatagccc aaaaaatacc accaaccatc atataggcaa
      661 ctgggatgaa aatagctcct atttttttct tcactagcaa agcactagct agtccaaaaa
      721 tagagaacac agcgccccaa actccatacc agagagtcgt cacaagacta tagagcaact
      781 gattggaatc aaataattct tttaaggcac cactataatc tccaatataa atttcctgat
      841 aaggagtcac taaaagatta attcctaata ataataaata ggggagaaaa aagactagaa
      901 aagaagaaat aattgcagta ctacccacaa tagccagata cttcttttta gaaattcgac
      961 acaattgtgc tgttagaaaa tgactctcag catcctctat tatctgacta gaatagggca
     1021 gtgtacagat aagtgcagct actaggctaa tcggtgaaaa tcctcgaata gaagaagcgg
     1081 caaaaaaaat cgataaacct tcaattttat aaataccatt gaaagcaagg aaatttccta
     1141 aactcatgca aagaagggtc aaaaataaag acatataaaa tcgaggtgat tgaacgactc
     1201 gtacaagatt acaaatgaaa aattccatcc ttactcctcc ttataataaa aatagggtgt
     1261 agcattcttt tttcatgcta cacccacaat caaccatctt taaggcttac tctgacaagt
     1321 aagttaataa gaatctggac tccaagaacc tgaagtatga attcttacat gatttctaaa
     1381 ttgtggcgcc atagctaatc tagtaccaga accaatataa ttgtcaccac ctccattata
     1441 gtacatgaca atcctagagc cagaccccaa tgaatatacc ggggtaatat ctgacccact
     1501 ataggcgcta cgaatagagg tatttaacct tttaccgcca ccagtgctgt cattattatt
     1561 aattccagca gaggcgtttt ctttctcaag ctcactagtt gtatccgaac cccaacgcgg
     1621 caacgttaaa tcataatttc caatctgtct agctaaaact gtagggagaa acaaactaga
     1681 tgcaattgta actacgcaaa aaaggaatat atttttcttc ttttcatgac atcgtcctcc
     1741 taagaagtgg taaacaagat agttgattga tcaatttgat acatattaca tttt
//
LOCUS       DAVLTM010000187         1772 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLTM010000187 DAVLTM010000000
VERSION     DAVLTM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.188"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..677)
                     /locus_tag="VYN16_002064"
     CDS             complement(<1..677)
                     /locus_tag="VYN16_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1404513.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKY"
     gene            complement(733..1755)
                     /locus_tag="VYN16_002065"
     CDS             complement(733..1755)
                     /locus_tag="VYN16_002065"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1404514.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          508 a          390 c          310 g          564 t
ORIGIN      
        1 tggtatttgc tattggattc caatttttct ttttgccatt ttttaaaaac ctcgttatat
       61 tcttttgttg taacaatatc tttttgcaat ttcattcctt taaagatata tggttccccc
      121 ttaataccaa cttgtgagta tggttttgag aatggtacta cgttacttac aactggagaa
      181 ccaccagatg aagctgttgg catcaataat gaactatctg tcgaccaagc ttgagctttg
      241 gcatattttt catatctctt ctctaggtca gtggtctcag aaacagcatc ttctaacaat
      301 ttcttatatt tatccaaacc aggtttagct acaacatcct tatcttttcc tttcgtaatg
      361 ccaaggtgtt tcatggcaga accagatttt ggatccataa tattcaagta agacgctgga
      421 tcttgatagc ttggagccca tcctgtactg ttcaaatcat agtctttttg agaaggagca
      481 acattgccgt atttatcatt ttccatcaaa ccatcaataa catttccaat aacgtctgtc
      541 ctcgatgttc gagtcgctat actgtagccc aatgatgctg gatctactgc atagacataa
      601 gaaaatgttg tcggtgcatc tgcttcttta tcagtttttc cacaagccac taaaatagct
      661 gacgtgctca ggaccactcc tgctgttaag agccactttt tctatttcat aaaaaatctc
      721 ctttggttta ttttaatcta cttttacaat ccaaccttct ggcgcttcaa tatcgccaaa
      781 ctgaataccc gtcaattcat tatataattt acgcgtcaca ggacctactt ctgtttcact
      841 atagaataca tggaaatcat caccatgttg aatacctcca attggagaaa taaccgctgc
      901 tgtaccacag gcacctgcct ctacaaaacg gtcaagatta tcaattggaa catcaccctc
      961 aataggagtt aatcccaagc gatgttctgc caaataaagc aaggaatact tggtaataga
     1021 tggcaagata gatggactca atggtgttac aaattcatta tcagctgtaa ttccaaagaa
     1081 attagctgat ccgacttctt caatctttgt atgagttgat gggtccagat agataacatc
     1141 tgagaaatgg cgtgacttgg ccatttttcc tggtaagaga cttgcagcat agtttccacc
     1201 aaccttagcc gcacctgtac catttggtgc tgcacggtcg tactcatcct gaatcaagaa
     1261 gttggttggg accaaaccac ctttaaagta atttccaact ggcatagcaa agatggtgaa
     1321 aatgtactct tctgccggtt ttaccccgat aatatctccg acaccaatca aaagagggcg
     1381 aagatataaa gttccaccta ttccgtatgg tggtacgtat tcttcattcg cacggacaac
     1441 tgctttacaa gcttctacaa acatgtctgt cggaacttgt ggcatcaaga gacggtcaca
     1501 tgtacgttgc aggcgtttag cattttcatc aggacggaac agttgaacac tgccatcctt
     1561 agtacgataa gctttcaaac cttcaaatgc ttgttgtcca tagtgaagac ttggagaaga
     1621 ctctgaaata tgcaaagttg catcctctgt aagctctcct tgatcccatt gtccattttt
     1681 gaaatgagca agatagcgat aaggtaattt catataggaa aaaccgaggt tttcccaatc
     1741 aatcgttact gtcatattcc actccttatt ct
//
LOCUS       DAVLTM010000188         1753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLTM010000188 DAVLTM010000000
VERSION     DAVLTM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.189"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(30..1157)
                     /locus_tag="VYN16_002066"
     CDS             complement(30..1157)
                     /locus_tag="VYN16_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1404515.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFTTEYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          513 a          365 c          248 g          627 t
ORIGIN      
        1 ttatcaattt tgtcgcaggg aggaatctgt taacgcaccc attcaccatt atcattgact
       61 ctatagccat ctatacttgt attgaccgct aactcacccg atgcatttac ataataccat
      121 ttaccaccaa cttggaacca ttgattgact ttcatagaac cattgctgtt gaggtagtac
      181 catgaaccat taacttgtac ccaacctgtt gccatggagc catcagtatt ataaaaatac
      241 cacataccat tttcttgttt ccagcctgtt gttggagcaa ctgctttagc tggttctact
      301 gctacatctg ttccttggtt agatgtaaca gatacaggat atgaaggaat agatgattgc
      361 tcaggaacaa caactttttc aggttctctc gtccctctcc ttatacgtct ttttaccatc
      421 tctttagtaa tttgacgaga agtagtttct tcaattgttc catcacgttc atctacagca
      481 tagattgtag taagagtaat tttaccaatt tctcctactt cttgaccttt atcaagagtt
      541 gggtcatcga gatattctgt ttcgattgga atttcttgga caagaacttg gggcttggtt
      601 ccttttttaa caactcttgt ttgagagtct tctttttgac ttaaagtact ctcagttact
      661 tgtccactct ttccatctac atcataagtt atcgttgtaa ctgttttccc attctttcct
      721 agagtaatct cttgctcctg tcctgcagaa aggtcattgt ctgcttcata ttcagtagta
      781 aatggaacaa gaacttcttc aaccttgctt ttagctggaa ctttgataac tgtatccgtg
      841 gcttcttttc tatcaacagt aacctgttcg gtaacataac cagtctctgg attaacatcg
      901 taggtccttg tcgtagttac atagccatcc tctccatcaa ttgtaacagg attttcacta
      961 cggtcttttg tttcatcttt ttcataacga attcgcgtac ttgaaatttt cttggttact
     1021 accttaggtt tagtcgctac ttttacaata atatccccat tgtcagcgtc atcatactct
     1081 attccctctt ctttatctct agtatcatct ctgacatatt gaatcccatc agcagcatga
     1141 acaaaacttg tattcagatt cctcctaaaa ataaagttag cccgattacc gcagaaccaa
     1201 aaatctttcc gagtttacgt attgcatagc gcttattagt attagatttt gccattacat
     1261 cctacttcta gtatagcatc ttttctatca aacgttaaac aatatacttt atatataaaa
     1321 tagacttaga atgatatatt gattactgaa ctaacacttt aactatatcg taatcaatct
     1381 catatataaa ggattgcaga catcttatct aaatacatgc taatatattt agatacaaac
     1441 attccaactt gataattttc actcatcttt catcattcct tatacaacta tgcagtataa
     1501 atagaatagt tttctcatca gaatgagact attttaatat tagataattt gatagaaaaa
     1561 acatgaataa aattggttga atttaattcc ctaactcgat agaggattct attttaaatt
     1621 taacaacagc taggattaca ctataaaaag tacattacca cctgttcggc tggagaatct
     1681 agtcgattag ctccctctta gttacaagct taaagcgatt ttttaactaa cactaaacca
     1741 gattaaagaa gga
//
LOCUS       DAVLTM010000189         1721 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLTM010000189 DAVLTM010000000
VERSION     DAVLTM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.190"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..692
                     /locus_tag="VYN16_002067"
     CDS             <1..692
                     /locus_tag="VYN16_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1404516.1"
                     /translation="KHAKGGRKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNL
                     IRRSQWVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKQLANDSGKKKFHVMK
                     AQAIVTSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILTDSGYQGLMKIYPQAQT
                     PRKSSKLKPLTAEDKACNHALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNL
                     IAGIINHELGF"
     gene            complement(1430..1690)
                     /locus_tag="VYN16_002068"
     CDS             complement(1430..1690)
                     /locus_tag="VYN16_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404517.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
BASE COUNT          580 a          323 c          309 g          509 t
ORIGIN      
        1 ttaaacacgc aaaaggtgga cgaaaaccta aattaagcct agaagacctt cttatggcca
       61 ctcttcaata tgtgcgagaa tatcgaactt atgaacaaat tgcggctgat tttggtattc
      121 acgaaagcaa cttaatccgt cggagccaat gggttgaagt aactcttgtt caaagtggtg
      181 ttacgatttc aagaactcct ctcagctctg aggacacggt aatgattgat gcgacggaag
      241 taaaaatcaa tcgccctaaa aaacaattag cgaatgattc tggtaaaaag aaatttcacg
      301 ttatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcactg
      361 tgaactattg tcatgatatg aagttgttca aaatgagtcg cagaaatatc ggacaagctg
      421 gtaaaatctt gactgacagt ggttatcaag ggctcatgaa gatatatcct caagcacaaa
      481 ctccacgtaa atccagcaaa ctcaagccgc taacagctga agataaagcc tgtaaccatg
      541 cgctatccaa ggagagaagc aaggttgaga acatctttgc caaagtagaa acgtttaaaa
      601 tgttttcaac aacctatcga aatcatcgta aacgctttgg attacgaatg aatttgattg
      661 ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctatt atatatgcac
      721 agacatatga aatctttttg agacagcagt tatgatatat ttatccattc acaacatctt
      781 ctgtactaat actacaatat tggtacattt tgattacaca atcgtttcaa aaaatatgat
      841 ggaagcctga gtattttata gtaattccct tcctatcaac tagatcaacc agttgagtgt
      901 tgaatgttga aacaccctag cctcagcaaa ggactatgat cagcatttcc tagccaatag
      961 gacgctccaa atcacctatt ctaagtccat cactagcttg gtcagtaaga tacagactgc
     1021 aaggcgagac tgatgtggtt gatgagattt tggaagagta ttacaatcat agattcacca
     1081 gtagacttga ccgcagattt aactatagta gattgaaact agaatagtac acctctactt
     1141 ctaaaacatt gttagaaatc gatttgactg tcctgatcaa tttgtcctgt tctcgtttca
     1201 ttttactata ctaaaaaacc acctgaaaca aatcaagtga ctagaggata aaaacaaaaa
     1261 ctgggcaaaa agttttgaaa agcaaaaaac gcataatatc aggtcttgaa aaaccttgat
     1321 actatacgtt tgattgtggg aagattgact tcattttctc ctgaaattga agttttttct
     1381 agtttccaga tatagacgaa ttcaatatag ccatctcgct acaattattt taaccatcga
     1441 gaaaattttt cagaatttca aaaatttcat tttgatccaa tgaaatgtca ttattttgaa
     1501 gaatagttga cttcacaggc tctaattcta tcacaaagcg gtgcatgtct gcttttaccg
     1561 cactacttga tgttatagcc gataactttt catctcctag caaggaagct gtcaaacggg
     1621 taaggtcatt caaatgcttt ttaatggact tacttaaacc ttgagcacct gtggcagagc
     1681 gctccctcat atccagccaa gctttagcct taaaaataat t
//
LOCUS       DAVLTM010000190         1713 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLTM010000190 DAVLTM010000000
VERSION     DAVLTM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.191"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            99..1127
                     /gene="queA"
                     /locus_tag="VYN16_002069"
     CDS             99..1127
                     /gene="queA"
                     /locus_tag="VYN16_002069"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1404518.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
BASE COUNT          493 a          391 c          329 g          500 t
ORIGIN      
        1 tgtcaagtga aaacgattta atttctaaaa aaagagcgtc caaacttgaa acgtgttata
       61 atggatagga ttagaactta gaaagaatga acagatatat gaatacagct gattttgatt
      121 tccacttgcc agaggaattg attgcccaaa cgccccttga aaaacgtgat gcctccaaac
      181 tcctcatcgt caaccgtgag acaggagaaa tgcaagataa acacttccac tctattattg
      241 atatgctgga acctggtgat gcccttgtca tgaacgacac ccgagttctc cctgcccgcc
      301 tctatggtca aaaagtggag acaggaggtc atgtggaact tctcctcctt aagaacacta
      361 gtggagacga gtgggaagtt ctggctaaac ctgccaaacg cctcaaggtc ggtactcgta
      421 tcagctttgg tgatggccgc ctcagcgctg tcgttacaga agaattgacc cacgggggac
      481 gcattgtccg ctttgaatac caaggaattt tcctagaagt cttggaaagt ctgggagaaa
      541 tgcctctgcc accttatatc cacgaaaaat tagatgaccg tgaacgttat caaaccgtct
      601 acgccaagga aagcggctct gctgcagcac cgactgctgg tcttcacttc accaaagaac
      661 tgctggcaga aatccaagct aagggtgttc atctagtcta tctgactctc catgtcggac
      721 tcggaacctt tagacctgtt tctgtggata atctggacga acacgaaatg cattcagagt
      781 tctatcaact ttctgaggaa gctgctgcca cccttcgctc tgtcaaagaa aatggtggtc
      841 gcgtcatcgc tgtcggaacc acttctatcc gcaccttgga aactattggt tccaagtttg
      901 atgggcaaat ccaagcagat tctggttgga ccaatatctt tatcaaacct gggtatgagt
      961 ggaaggtcgt ggatgccttc tcaaccaact tccatctgcc aaaatcaact ctggtcatgt
     1021 tggtttctgc ctttgcaggc cgtgaattag tcttagatgc ctaccaccat gcgatccaag
     1081 aacactaccg cttcttcagt tttggtgact ccatgtttat ttattgagaa agaatttctc
     1141 taaatcttct aataccaata aatcgctaag atattatttc aaagaacatc tacaattgaa
     1201 actctagcta gctgtagaag aggcctagta cattgaaatt aaaatgcttc cccctagctt
     1261 cgaaaatatt gccatagatt gcgttgactc tccaaattga ttcatctata ttttatttca
     1321 gcttcctata ttttcttcgc tgtttgtaaa tcaaaatgca agacacatga gtagcaccat
     1381 atttgttact cttatctgtc ctctcaagag actattatga gttatttcag aatcattcac
     1441 tactttgacc ctgactctcc ttagtctcaa aatcaaagac ttatactctt caaaaatctc
     1501 ttcaaaccgc gtcaacgtca ccttggatta tatatgtgac tgacttcgtc agttctatct
     1561 acaacctcaa agcagtactt tgagcaacct gcgactagtt ttctagtttg ctctttgatt
     1621 ttcattgagt attaaacaaa aagtgaacaa agctgaattc taatgtacag aagactaggc
     1681 ttgttcactt ttttatagtc gctataagat gac
//
LOCUS       DAVLTM010000191         1699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLTM010000191 DAVLTM010000000
VERSION     DAVLTM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.192"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..77
                     /locus_tag="VYN16_002070"
     CDS             <1..77
                     /locus_tag="VYN16_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1404519.1"
                     /translation="QQVIPKAAFGYIASGAEDTFTSFQ"
     gene            205..1695
                     /gene="lysS"
                     /locus_tag="VYN16_002071"
     CDS             205..1695
                     /gene="lysS"
                     /locus_tag="VYN16_002071"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1404520.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLKEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT          516 a          373 c          351 g          459 t
ORIGIN      
        1 cgcaacaagt tattcctaaa gcagcatttg gctatatcgc tagtggggcg gaagatactt
       61 tcacttcttt ccagtgattt tagcgtcagg ttctttttag tttttaaaga ttatccgtga
      121 atttcttgct tatttatgat aaaatgggag tatcgcaaaa aatgactcat cgtattcaat
      181 tttgagtaaa actaggagga tcccatgtct acagaacata tggaagaact aaatgaccag
      241 cagatcgttc gccgtgaaaa aatggctgcg ctccgcgaac aaggaatcga tcctttcgga
      301 aaacgttttg aacgtactgc aaattcacaa gaattaaaag ataaatatgc caacctcgat
      361 aaagaacaat tacacgataa aaacgaaaca gctactatcg caggacgctt ggtaaccaaa
      421 cgtggtaaag gaaaagttgg ttttgcccac cttcaagacc gcgaaggcca gattcagatc
      481 tacgttcgta aggatgctgt cggtgaagaa aactacgaaa tcttcaaaaa agcagacctt
      541 ggtgacttcc ttggtgtcga aggtgaagtg atgcgtacgg atatgggaga actctctatc
      601 aaggcaaccc acatcacaca cttgtctaag gctcttcgtc ctcttcctga gaaattccat
      661 ggtttgacag acgttgaaac aatttaccgt aaacgttacc ttgacttgat ttctaatcgt
      721 gaaagctttg aacgctttgt cactcgttca aaaatcatct ctgaaatccg tcgttacctt
      781 gaccaaaaag gattccttga agtggaaaca cctgttcttc ataatgaagc cggtggtgct
      841 gctgcccgtc catttatcac ccaccacaat gcccaaaaca ttgacatggt gcttcgtatc
      901 gcgactgagc ttcacttaaa acgccttatc gtgggtggta tggaacgtgt ctatgaaatt
      961 ggccgtatct tccgtaacga aggaatggat gctactcata accctgagtt cacttctatc
     1021 gaagtttacc aagcttatgc agacttccaa gatatcatgg acttgacgga aggcattatc
     1081 caacatgctg ctaaatcagt taagggcgat ggcccagtca actaccaagg tactgaaatc
     1141 aagattaacg aaccatttaa gcgtgttcat atggtggatg ctatcagaga aattactggt
     1201 gtcgatttct ggcaagacat gactttgaaa gaagctaaag ctatcgctgc tgagaagaaa
     1261 gttccagttg agaaacacta cactgaggtt ggtcacatca tcaatgcctt ctttgaagag
     1321 tttgttgaag aaactttaat ccaaccaacc tttgtctatg gacatccagt agctgtatct
     1381 ccactcgcta agaaaaatcc tgaagaccaa cgctttactg accgtttcga gctctttatc
     1441 atgactaagg agtacggtaa tgcctttact gagctcaacg acccaatcga ccaacttagc
     1501 cgttttgaag cccaagctaa agctaaagaa cttggtgatg atgaagcgac aggcatcgac
     1561 tatgactaca tcgaggctct tgaatacggt atgccaccaa caggtggttt gggaatcggt
     1621 atcgaccgtc tctgcatgct cctcactgat acaacaacta tccgtgatgt attgctcttc
     1681 ccaacaatga aataaattc
//
LOCUS       DAVLTM010000192         1673 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLTM010000192 DAVLTM010000000
VERSION     DAVLTM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.193"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(23..205)
                     /locus_tag="VYN16_002072"
     CDS             complement(23..205)
                     /locus_tag="VYN16_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1404521.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            540..>1673
                     /gene="pcpA"
                     /locus_tag="VYN16_002073"
     CDS             540..>1673
                     /gene="pcpA"
                     /locus_tag="VYN16_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1404522.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLP"
BASE COUNT          603 a          234 c          265 g          571 t
ORIGIN      
        1 atcagttaat tgtttacttg cttcataatt tcgcagggag tctattgact ctttggtagg
       61 tgtcaatgtt tttttcatct atcccgagaa ttattttccc gccatttgta tttgcaaatg
      121 ctgagtaggt ttcccagaaa gactctggaa gattgttttt agcttttttg tattctaaat
      181 caaccccttc aaattttaag tccatatttt tcctttacat ctgttttttg tggttctggt
      241 atttgttcaa gttgagtgat aatatagcga attgaatttc gagagttttt actcagttaa
      301 tttctttttt aacccacttt aatttctttt ttaacacggg ttaaaaaaga aattaaagtg
      361 ggttaatttt ccttgactta aaatttaaaa atgatatact gtgtttatgg agagatgaca
      421 atttataaaa tgtactactc tattctacta gataatgatt gaataaaact ttttataaca
      481 acggctagca aagttataat tttatatcta ttagaaaata aatatgtaag gaaatttaaa
      541 tgaaaaaaac tacaatatta tcattaacta cagctgcggt tattttagca gcatatgtcc
      601 ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag actaaagttg
      661 gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa ggtaacatag
      721 gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt caagatggaa
      781 aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc gttactgaag
      841 tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc tactatccta
      901 gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc catggaagta
      961 aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa gatagagctt
     1021 ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat attggaacaa
     1081 gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt tcaaaattag
     1141 aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta acattaccaa
     1201 aatcggttaa aacattagga agtaatctat ttagactcac tactagctta aaacatgttg
     1261 atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt tcaaaagata
     1321 aaactcaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa acgcctaaag
     1381 agacaaaaga acttgcatca tattcgttta ataaaaattc ttacttgaaa aaactcgaat
     1441 tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc ggatgcgatt aaacttgaag
     1501 aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac ggtaatttag
     1561 aattaaaaga acttatatta ccagataatg ttaaaaattt tggtaaacac gttatgaacg
     1621 gtttaccaaa attaaaaagt ttaacaattg gtaataatat caactcattg ccg
//
LOCUS       DAVLTM010000193         1664 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLTM010000193 DAVLTM010000000
VERSION     DAVLTM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.194"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            21..329
                     /locus_tag="VYN16_002074"
     CDS             21..329
                     /locus_tag="VYN16_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1404523.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            346..1644
                     /locus_tag="VYN16_002075"
     CDS             346..1644
                     /locus_tag="VYN16_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1404524.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
BASE COUNT          467 a          234 c          369 g          594 t
ORIGIN      
        1 acatagagga ggaaaacata atggtgaaga ttggtttgtt ttgtgcagca ggtttttcta
       61 ctggtatgct tgtaaataat atgaaaattg cagcgcaatc tagtggagtt gaggcagaaa
      121 tagaggcgtt ttctcagtct aaattagcgg attatgcgcc aaatatagat gttgcactat
      181 tgggtccaca agttgcttat acattagata aatcaaaaga aatttgtgat aagtgtgatg
      241 ttccgatagc tgttattccg atgatggact atggtatgtt agatgggaaa aaagtattag
      301 atttggccct atctttgatt agtgggtaag aaaaggagat ttattatgtc aaagatggat
      361 gttcagaaaa tcattgcacc gatgatgaag tttgtgaata tgcgtggcat tatagctcta
      421 aaagatggga tgttagcaat tttgccattg acagtagttg gcagtttgtt cttgattatg
      481 ggacaattgc cgttcgaagg attaaataag agcattgcta gtgtttttgg agctaattgg
      541 acagagccgt ttatgcaagt atattcagga acttttgcta ttatgggtct aatttcttgt
      601 ttttcaattg cctattctta tgctaagaat agcggagtag aggctttacc agctggagtt
      661 ctatctgtat ctgcattctt tattttgcta agatcatctt atatccctaa acaaggtgag
      721 gcgattgggg acgctattag taaagtttgg tttggaggcc aaggaattat cggtgctatc
      781 attataggtt tggtagtagg aagtatttat accttcttta taaagagaaa aattgttatt
      841 aagatgccag aacaagttcc acaagctatt gccaaacagt ttgaagcaat gattccagca
      901 tttgtaattt tcttatcttc tatgattgta tatattttag cgaagtcatt gactaatggc
      961 ggaacattca tagaaatgat ttattctgct attcaagttc cgttgcaagg tttaactgga
     1021 tctttgtatg gtgctattgg aattgcattc tttatatcat ttttgtggtg gtttggtgtt
     1081 catgggcaat cggtagtaaa tggagtagtg acagctctgc ttttatctaa tcttgatgct
     1141 aataaagcta tgttagcctc tggtaatcta tcattagaaa atggtgcaca tattgttact
     1201 caacaatttt tagattcatt tttaattcta tcaggttcag ggattacgtt tggtcttgta
     1261 gttgccatgc tttttgcagc aaaatcaaaa caataccaag ccttaggaaa agttgcagct
     1321 tttccagcaa tatttaacgt aaatgagcca gttgtatttg gatttccgat tgtcatgaat
     1381 ccagttatgt ttgtaccttt cattcttgtt cctgtacttg cagctgtgat agtatatgga
     1441 gctattgcaa caggtttcat gcagccattc tcaggggtaa cattgccttg gagtacacca
     1501 gctattctat caggattttt ggtgggtgga tggcaaggtg ttgtgactca gttattagta
     1561 ttggtgatat ctacagtggt ttactttcca ttctttaaga tacaagatcg tttagcttac
     1621 caaaatgaaa tcaaacaatc ttagaggtat ttgtgtgtta ctgt
//
LOCUS       DAVLTM010000194         1631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLTM010000194 DAVLTM010000000
VERSION     DAVLTM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.195"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..588
                     /locus_tag="VYN16_002076"
     CDS             <1..588
                     /locus_tag="VYN16_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1404525.1"
                     /translation="VLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTC
                     ALLAEEICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMI
                     QTFHVHQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVR
                     RFALSQVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            601..1581
                     /locus_tag="VYN16_002077"
     CDS             601..1581
                     /locus_tag="VYN16_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1404526.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          594 a          180 c          330 g          527 t
ORIGIN      
        1 gtattaaata gtggagatga cttaaatgta cgtttgtcaa ttaattatga aattaagaga
       61 aaaattgttc aggaagcagt aaaattggtg tcagataatg aaacaataat gatagaatct
      121 ggatcgacct gtgctttact tgctgaggaa atttgcaagc aaaaaagaaa tgttacgatt
      181 gtaacaaatt cattttttat agcaaatttt gtgagagctt atgattcatg tcgtattatt
      241 gttcttggtg gagagtttca gaaggattca caggttactg tgggaccttt attaaaagaa
      301 atgatacaga cttttcatgt gcatcaagct tttgttggga cagatggtta cgataaagag
      361 atgggcttta caggaaaaga tttaatgcgc agtgaggtag ttcaatatat ttcagcagcg
      421 tcggataaag tcattgtact aactgactca agtaaatttg ataaaagggg tacagtaaga
      481 agatttgctt taagtcaagt ctatgaagta ataacagacg aaaaactttc taaacaaaat
      541 atagctacat tagaaaatgc tgggataatg gttaaggtag tttcgtaaga ggttaaatgt
      601 atgaatcaag ataggaataa actgctctct aaaattgctt atctgtatta tattgaaaac
      661 ttaaatcagt cacaaatagc agcaaaatta ggaatttata gaacctctat tagtagaatg
      721 ttaacagaag caaggaatgc aggaattgtt aaaattgaaa tagagaattt tgataccaat
      781 atgtttaagt tggaaaatta tgtaaaagaa aaatatggtc tggaaagttt agaaattatt
      841 ccaaatgaat ttgatgataa tccaacaatt ttatctgaaa gaatttctca agttgcagca
      901 ggtgtcctta ggaatctaat tgatgataat atgaaaattg gcttttcttg ggggaaaagt
      961 ttaagtaatt tagtagattt aattcacagt aaaagtgtcc gaaatgttca cttctatcct
     1021 ctagcaggtg gtcctagtca catacacgct aaataccatg tgaatacact gatttatgaa
     1081 atgtctagaa aatttcatgg agagtgtaca tttatgaatg caacgattgt gcaagaaaat
     1141 aaattgttag cagatggtat tttgcaatca agatattttg aaaatttgaa aaatagttgg
     1201 aaagatttag atatagctgt agtcggaatt ggtgatttta gcaataaagg aaagcatcaa
     1261 tggttagaca tgcttacaga ggatgacttt aaagaattga ccaaagttaa aactgtagga
     1321 gaaatttgtt gtcgattttt tgattcaaaa ggtaaagaag tctatgaaaa cttacaggaa
     1381 agaacgatag caatctcttt ggaagattta aaaaatattc ctcaaagttt agctgttgct
     1441 tacggtgata cgaaagtatc ttcgattctt tctgtcttgc gtgctaattt agtaaatcat
     1501 ttgattacag acaaaaatac aattttaaaa gttttggaag aagatgggga tttgactttt
     1561 agagagattc taggtgagtg aaaatgatag actgattcag tttatcgttt ttctttttag
     1621 ttgattgcac a
//
LOCUS       DAVLTM010000195         1624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLTM010000195 DAVLTM010000000
VERSION     DAVLTM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.196"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            39..779
                     /locus_tag="VYN16_002078"
     CDS             39..779
                     /locus_tag="VYN16_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404527.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            782..>1624
                     /locus_tag="VYN16_002079"
     CDS             782..>1624
                     /locus_tag="VYN16_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1404528.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSY
                     IKSIS"
BASE COUNT          528 a          209 c          308 g          579 t
ORIGIN      
        1 ggaacacttt tcaaaattaa gtgattggag gcaagaatat ggataaagat tatatattaa
       61 aagtgaaagg gctgtatcat caatttttac taggaaataa taaaacgttg caagtgctga
      121 aaaatgtttc tctttctgct tcgagaggag aatttataag tattctagga attagtggtt
      181 ctggaaagtc aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag
      241 taattttaaa tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac
      301 gtaacgaagt atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag
      361 aaaatatagc acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa
      421 acttactcaa aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg
      481 gtggagaaca acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat
      541 ttgctgatga gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat
      601 tgagagagtt agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg
      661 cctcgaaaac tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata
      721 gacctagcgg ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga
      781 gatgctcaga ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc
      841 accagtaatt tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt
      901 tgagaataat tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc
      961 gatagtattc ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt
     1021 tgaaattttt agagatgatt acgaacttct ggaggtgttg ggagcaagtc gccttcaact
     1081 atcatttctt gtggggggac agatttttat catttcctcg attattagtt ttattgcata
     1141 tctatgctct atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga
     1201 aaatatatta cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt
     1261 gttgatttca tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa
     1321 taggaagagt tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc
     1381 tggttttagt gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt
     1441 agcgaaggcc cagataatat tcaatattgt aatcttagat attgtgatta tttatcaatt
     1501 atctcctttt atacagtcat gttttatcaa actactttct ataattatat ttagaaataa
     1561 ttttatgttc attgtttcga agtggaattt actatattgt aagtcttata taaaatctat
     1621 cagt
//
LOCUS       DAVLTM010000196         1608 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLTM010000196 DAVLTM010000000
VERSION     DAVLTM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.197"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..425
                     /locus_tag="VYN16_002080"
     CDS             <1..425
                     /locus_tag="VYN16_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV1404529.1"
                     /translation="NCKLLIRLHPNHSQFCRNILFNQDIINVTFFSDMQELILLADVL
                     LTDYSSSIFDFILLNKPYVRYVNDLEEYSKLRGLSDTYYELPDTIISTSEELYTLLPE
                     KINDFDYAAINQYRNGTLSPMFNGRASENIGRRIIQEL"
     gene            461..1489
                     /locus_tag="VYN16_002081"
     CDS             461..1489
                     /locus_tag="VYN16_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1404530.1"
                     /translation="MKNNVLKIGSGAIHQISSTLAQNNISGKILYCADPIVDNLYGSL
                     VRSQIEEIGRLKEESCNYNTIAYAMNIAERVIATDIDCIVGMGGGRVLDVCKYASYIS
                     KRPYLSIPTTAANDGIASPVAVLKRQDDRPKSLGAAIPSMTLIDIDVIASGPIQNIKA
                     GIGDTISNYTALKDWELAVNRGKDEMHGFAYLMSQNSLDALMKTKYNSITPDFIEVLI
                     NSLVLSGIAMDFAGSSRPVSGSEHLFSHALDYYGSKRNLHGIQVALGTVAVLKIIEDP
                     ITPVTDYLQRFEVNINPKQMGIDEDLFVYCMQHATTMRNNRYTYLHEADLSTARVKQI
                     YKELVNEL"
     gene            1505..>1608
                     /locus_tag="VYN16_002082"
     CDS             1505..>1608
                     /locus_tag="VYN16_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEV1404531.1"
                     /translation="MKALILAAGLGTRLAPITNDVPKSLVPVNGKPIL"
BASE COUNT          552 a          230 c          309 g          517 t
ORIGIN      
        1 agaattgtaa attattaatt aggttgcatc ctaaccactc acaattttgt agaaatattt
       61 tatttaatca ggatataatt aatgttacat tcttttcaga tatgcaggaa ctcatattgt
      121 tggcagatgt tctactaaca gattattctt catctatatt tgattttata ctcttaaata
      181 agccgtatgt aagatatgtg aatgatcttg aggaatattc taaacttaga ggattatcgg
      241 atacatatta tgagctacct gacactatca tttcgacatc ggaggaactg tataccctcc
      301 tgcctgaaaa aataaatgat tttgattatg ctgctataaa tcaatataga aatggaaccc
      361 ttagtccaat gtttaatgga agagctagtg aaaatatagg aaggagaata attcaagaat
      421 tgtaaagaaa atcattgaat tattttttag gtgaaaaggt ttgaaaaata atgtattaaa
      481 aataggcagt ggggctattc atcaaatatc ctctacactt gctcaaaata atatatcagg
      541 taagattcta tattgtgctg atccaattgt tgacaatcta tatggctctt tagttaggtc
      601 acaaattgag gagataggtc gattaaaaga agaaagttgt aattataata cgattgctta
      661 cgcaatgaat atagccgagc gagttattgc aacagatatt gattgtattg tgggaatggg
      721 aggtggtcgc gttttagatg tttgtaaata tgcatcttat atttcaaaac ggccatattt
      781 atcaattccc accactgcag cgaatgatgg gatagcttct ccagtagcag ttttgaaaag
      841 acaagatgat agacctaaaa gtttgggtgc ggcaattcca tctatgactc ttatcgatat
      901 tgatgttatt gcatcaggac ccatacagaa tattaaagcg ggcattggtg atacgatttc
      961 aaattatacg gcgttgaaag attgggaatt agctgttaac cgaggcaagg atgaaatgca
     1021 tggctttgcc tatttaatgt cccaaaactc cttggacgct ttgatgaaaa caaagtataa
     1081 ttctattacg cctgatttca ttgaagtttt gataaattca ttagttttat caggtattgc
     1141 aatggatttt gcaggaagta gtagaccagt tagtggttca gaacacttat ttagtcatgc
     1201 cttggattat tatggttcca agagaaacct tcatggtatt caggttgcat taggtacagt
     1261 tgcagtatta aaaataattg aagatccgat tacaccagtg acggattact tgcaaagatt
     1321 tgaggtaaat atcaatccaa aacaaatggg aatcgatgaa gacctatttg tttattgtat
     1381 gcaacatgct acaacaatga gaaataatcg ctatacatat cttcatgaag ctgatttaag
     1441 cacagctagg gtgaaacaaa tatataaaga attagtaaat gagttgtaga aaagagagga
     1501 aaaaatgaaa gcacttattt tagcagcagg tttaggaaca cgtttggctc ctatcactaa
     1561 tgacgtccca aaatcattag ttcctgttaa tggaaaacct attcttat
//
LOCUS       DAVLTM010000197         1598 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLTM010000197 DAVLTM010000000
VERSION     DAVLTM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.198"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            297..902
                     /locus_tag="VYN16_002083"
     CDS             297..902
                     /locus_tag="VYN16_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1404532.1"
                     /translation="MKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTP
                     ARTPNSVPFGRIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQ
                     KNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASN
                     RVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            1201..1560
                     /locus_tag="VYN16_002084"
     CDS             1201..1560
                     /locus_tag="VYN16_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1404533.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
BASE COUNT          550 a          192 c          301 g          555 t
ORIGIN      
        1 tttataaaaa attgggtatt tcaattattg cgagtacttt attggctagc cagttatcga
       61 cagtatctgc tttgagtgtt atttctagta caggtgaaga atatgaggta agtgagacat
      121 tacaagatag tccaggaact aataattttt cacttcccaa tgtttcaccg acttatggtc
      181 aatattatac aaatcagtca gaagtcatta ttggtaaaaa tgatttagtt aaggtaaaaa
      241 atactttaca atatccttat tctacatcag cttatgtaga gtcagaattt cgtggtgtga
      301 aaaatggtaa agatgtaact tatcgaggga gtgccaattt tataaaggat aatgtcctta
      361 ttactgctgc tcataatatt tataaacatg agtttggtaa agatgcagaa gaactatatg
      421 ttactcctgc tcgtactcca aatagtgtgc cgtttggtcg gataaaagta aaagaagttc
      481 gttatttaaa ggaatttaga aatttaaatt ctaaggatgc aagggaatat gacttggctt
      541 tattaattct agaagagccc attggtgcaa aattagggac tttgggtctt cctactagtc
      601 aaaaaaattt gacaggaata actgtgacta tcacaggcta tccatcatat aattttaaaa
      661 ttcatcaaat gtatacagat aaaaaacaag ttttaagtga tgatggcatg ttcttggact
      721 accaagttga tactttagag gggtctagtg gatctacagt ttatgatgct agtaaccgcg
      781 tagtaggagt gcatacttta ggagatggag ctaatcaaat taacagtgca gttaaattaa
      841 atgaacgaaa tttgccattt atttattcgg ttcttaaagg ttactctctt gaaggatggt
      901 gaatgggtta ggtaatgaag gctaattgta aactgtgatg gatacttaac tttgtataat
      961 aggtggataa aagtctttaa aataggaaaa aacgcatatt attaggtgtt taaaaacctt
     1021 gataatatgc gttttataat ggaaagattt cttcaatttc gtcttgtata gtagattgaa
     1081 actataatag tacacctcta cttctaaaac attgttagaa attgatttga ctgtcctgat
     1141 cgatttgtcc tgttcttatt tcgttttact ataatgtaat cgtccaataa cgaagtgttt
     1201 ttggatagtc cgaaaagttt agcttcatat agagtaattg gtattagtga tagagtgtta
     1261 gaaattttaa aagaatatct tcttttagaa gaaggtgatt atatatttac aaataatggt
     1321 tctcccttaa tgataacttg tttcaattac tttttgaaaa acaggtttag gaaaagtgaa
     1381 attaagaaag atgattttgt attgactgct catgttttta gatattcgca tataagtttg
     1441 cttgctgaat tggaagtacc tataatagcg ataatggatc gtgtagatta tactaatgaa
     1501 acaaagattt taagtgttta tactcatgtt actgagaaga tgaagtcaaa tataacttaa
     1561 aaattagata aattagtttt ggaaaatgac taaccaaa
//
LOCUS       DAVLTM010000198         1597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLTM010000198 DAVLTM010000000
VERSION     DAVLTM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.199"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..1535
                     /locus_tag="VYN16_002085"
     CDS             <1..1535
                     /locus_tag="VYN16_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1404534.1"
                     /translation="NPKKTEAQKAMDEAEKEHKKKQAEFKKVRLEVIPSAKELEETRQ
                     KAKEAKEKEPELTKKVAEAKAKLEEAEKKVTEAKQKVDAEHAEEVAPQVKIAELENQV
                     HKLEQKLKEIDESDSEDYVKEGLRAPLQSELDTKKAKLLKLEELSGKIEELDAEIAEL
                     EVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAEADLKKAVDEPEKPAEESENPAP
                     APKPAPAPAPKPEQPAPAPAPKPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPA
                     PAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGW
                     LQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKA
                     SQWFKVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGS
                     MATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGE
                     WV"
BASE COUNT          605 a          300 c          356 g          336 t
ORIGIN      
        1 ataatccaaa aaaaactgaa gctcagaaag caatggatga ggctgagaaa gagcataaga
       61 aaaaacaagc agaatttaag aaagttagat tagaggtaat tcctagcgcg aaagagttag
      121 aagagactag acaaaaagca aaagaagcta aagaaaaaga accagaactt actaaaaaag
      181 tagcagaagc taaagcaaaa ttagaagaag ctgagaaaaa agttactgaa gccaaacaaa
      241 aagtggatgc agaacatgct gaagaagtcg ctcctcaagt taaaattgct gaattggaaa
      301 atcaagttca taaactagaa caaaagctca aagagattga tgagtctgat tcagaagatt
      361 atgttaaaga aggtctccgt gctcctcttc aatctgaatt ggataccaaa aaagctaaac
      421 tattgaaact tgaagagttg agtggtaaga ttgaagagtt agacgctgaa attgcagaac
      481 ttgaagttca acttaaagat gctgaaggaa acaataatgt agaagcctac tttaaagaag
      541 gtttagagaa aactactgct gagaaaaaag ctgaattaga aaaagctgaa gctgacctta
      601 agaaagcagt tgatgagcca gaaaaaccag ctgaagagtc tgagaatcca gctcctgcac
      661 caaaaccagc tccagctcct gcaccaaaac cagagcaacc agctccagct cctgcaccaa
      721 aaccagagaa gtcagcagat caacaagctg aagaagacta tgctcgtaga tcagaagaag
      781 aatataaccg cttgactcaa cagcaaccgc caaaagcaga aaaaccagct ccagctcctg
      841 caccaaaacc agagcaacca gctcccgcac caaaaacggg ctggaaacaa gaaaacggta
      901 tgtggtactt ctacaatact gatggttcaa tggctacagg ttggctacaa aacaacggtt
      961 catggtacta cctcaacagc aatggcgcta tggctacagg ttggctccaa tacaacggtt
     1021 catggtatta cctaaacgct aacggcgcta tggctacagg ttgggctaaa gtcaacggtt
     1081 catggtacta cctaaacgct aacggttcaa tggcaacagg ttgggtgaaa gatggagata
     1141 catggtacta tcttgaagca tcaggtgcta tgaaagcaag ccaatggttc aaagtatcag
     1201 ataaatggta ctatgtcaac agcaatggcg ctatggcgac aggatggctc caatacaatg
     1261 gttcatggta ctacctcaac gctaacggtg ctatggcaac aggttgggct aaagtcaacg
     1321 gttcatggta ctacctaaac gctaacggtt caatggcaac aggttgggtg aaagatggag
     1381 atacctggta ctatcttgaa gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat
     1441 cagataaatg gtactatgtc aatggcttag gtgcccttgc agtcaacaca actgtagatg
     1501 gctataaagt caatgccaat ggtgaatggg tttaagccga ttaaattaaa tcatgttaag
     1561 aacatttgac attttaattt tgaaacaaag ataaggt
//
LOCUS       DAVLTM010000199         1594 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLTM010000199 DAVLTM010000000
VERSION     DAVLTM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.200"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..228
                     /locus_tag="VYN16_002086"
                     /pseudo
     CDS             1..228
                     /locus_tag="VYN16_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818294.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            276..914
                     /locus_tag="VYN16_002087"
                     /pseudo
     CDS             276..914
                     /locus_tag="VYN16_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT          557 a          202 c          269 g          566 t
ORIGIN      
        1 tagatacata tcaatctaat aaaactttat cttcctttga tagtgataaa atatttgaga
       61 ttgtacttga gttggttcgg actgaaaagc tatctaattt atattttatt tcacttacta
      121 atgaaaaaga acattcttta tttgaatatt taactcaaaa aataaaaaac tcaactcata
      181 tacaagtgca agaacgttta caattgttgt ctctatatcc tactctaaaa aactttgcaa
      241 agtatgattg tatatttgtt gatgatgaag atttgatgca cacgctatta gacttatttg
      301 attggtttac tataattgat agaccaaaaa tatgtttgat aggttcacgt gtttggttcg
      361 aaaaaagaaa tcttttttat atacatttag attatagaat attggcactc aatactgccg
      421 aaaatttaga aagtggaaat agttcaattg taccagttaa agatggcaaa caaattttaa
      481 ttgatcaatt atttgatgga gaatgtgatg atatttattt acttactgtc cattcaccaa
      541 tgaataaagc cttagaaatt ttatcagaaa agtataaaca aatttctggg aaaaatatta
      601 aaattatcga gaaaagatat gatgaacttt tggagatgat tgagtctggc gatgtttcat
      661 ccagttttga tatgattaga atggatatgg cttggcttcc aacatttggg aaaaagtatt
      721 ttcaagagat tacaagcttt agacaaacaa aaagtattaa tcaaaatatt tctaagtcgg
      781 tgtctcgaga atatatgtat attgatcaga agcagtatac ttttccattg gatgttagta
      841 gtcagatttt agtatataga aaagacttat ttgaagattc atttttacaa agacgatact
      901 ttgaaaaaac aaaaagaaaa ttaactgtac caaaaagtta tcaagagttt gatgagttaa
      961 gtgaattttt tacattaaca gaaaatccat tttcacctac actttatggc catagtttag
     1021 ctttaagttc ttcagtgaga gcagcatgtg aatttattcc tcggtttcgt gagagattag
     1081 cacaatctag aatgaatctt gctgtacttc ctcaggtttt aactgagtat gagaaaagtt
     1141 atcagtttac cgaaaaatca tttaattctt cgtggaacga tttcgttacg aacttaaaca
     1201 gtggtaaaac ttcaatggaa ataatttttt caaattatac tagtccatta tttgatggtc
     1261 tcaatgtttc tgctcagttt gagtttgcaa ctgccactat acctggtaat actcctgtga
     1321 taggtggtgg ctcaattgga atttctaaat attcaaatag agtggaagaa tgtctaaact
     1381 ttattaattg gttatattct gaagagattt ctattttact aacatctctg ggtggttttt
     1441 taccatcaaa gtatgtcatg caaaatagga tgttacaatt tcaataccca tggttatcta
     1501 gtttagaaga tacatttagt attgggagta gaacaaaatg gtacggcttt aatactgatt
     1561 ttagatttga gcagatgtta gggcaagagt taat
//
LOCUS       DAVLTM010000200         1559 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLTM010000200 DAVLTM010000000
VERSION     DAVLTM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.201"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(127..1551)
                     /locus_tag="VYN16_002088"
     CDS             complement(127..1551)
                     /locus_tag="VYN16_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1404535.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVAEKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          450 a          350 c          280 g          479 t
ORIGIN      
        1 gaagtagagc tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt
       61 tctataatta ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt
      121 tacacgttat ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata
      181 tttaattcct tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg
      241 accacgctgg gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc
      301 aaaggctttt ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt
      361 ggcaaaggca atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa
      421 tggctcttcc catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag
      481 gagattgccc tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc
      541 aatcaagccc caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt
      601 gtcaaatgga tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt
      661 ctctgctaca ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta
      721 gctaaaggct cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac
      781 aagagctgca tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc
      841 aatacgttct ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc
      901 aatgatataa tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg
      961 aatccctgct tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg
     1021 tggcttaaac atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa
     1081 attaactgga taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac
     1141 aacagccagt ttgtttttac ttgttgcctc aaaaccacca ccttccattg cttgtccagt
     1201 gatacggaga ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc
     1261 aattgctgct ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt
     1321 atcgcgttct aaaatagctg ctgttttatg caaataagcc gcacgttcaa ctgctgataa
     1381 agctcgccat gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg
     1441 agtcatggct ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc
     1501 ttgttcagat gatttccatt ttccatttac taaattctga tatcttgtca aattcttgt
//
LOCUS       DAVLTM010000201         1553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLTM010000201 DAVLTM010000000
VERSION     DAVLTM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.202"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            16..606
                     /locus_tag="VYN16_002089"
     CDS             16..606
                     /locus_tag="VYN16_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1404536.1"
                     /translation="MPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYD
                     GFEPFISATMKEIDELVSEGVDILALDCTNRSRPGYDNITDFIHDIKVKYPNKLLMAD
                     ISTFEEGKVAVESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRIS
                     TPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV"
     gene            607..1539
                     /locus_tag="VYN16_002090"
     CDS             607..1539
                     /locus_tag="VYN16_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1404537.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
BASE COUNT          493 a          210 c          313 g          537 t
ORIGIN      
        1 ccaaatggag gagtgatgcc gttattagca aaagccgcct ttcaagcagg cgctaaaggt
       61 attcgtgcta attctgtccg cgatatttca gaaataaaag aagaagttga cttaccaatt
      121 ataggtatca taaaaagaga ttacgatggc tttgaacctt ttatttcagc aactatgaaa
      181 gagattgatg aattagttag tgaaggagta gacattttag ctttggattg tactaatcgt
      241 tcaagaccag gttatgataa tattactgac tttattcatg atattaaagt caagtatcca
      301 aacaaattat taatggctga tatttctact tttgaagaag ggaaagtagc tgttgaatcg
      361 ggagtagatt ttgtgggtac gacattatct ggttatacac catatagccc taaaaaagat
      421 aatcctgatt ttgagttagt tgaaagattg gtaaaagagc ttgacgtacc agttattgct
      481 gaaggaagaa taagtactcc ggaacaagct agaaaaatgt tagatttagg tgcttacgca
      541 gtggttgttg gaggagctat cactcgtcct ttggaaatag cgaagaagtt tattgaggta
      601 gtttagatga atcaagaaat attaaaaaag agtaagattc aattgacagt tgaacatatt
      661 caaaaatatt ggattttata tttaatgatg attccagcaa ctgttctact aattttattt
      721 acgtatggac caatgtacgg cattattatg gcctttcaag actttactgt cttcaaggga
      781 tatactggaa gtccatttgt cggtttaaaa cattttcaaa gattgttctc agatcctttg
      841 ttttatagat tgttcaagaa tacgttcatg gtaggggttt tagacttttt attctctttc
      901 ccagctccgc ttatttttgc actcatatta aacgaagtca gaaaagtaag atttaagagt
      961 gtagttcaat ctattagtta tcttcctcat tttattcccc tagtggttat ggttggtatt
     1021 atttttgaac tatttggttc atatggtatt attaacagtt tactttcatc acttggaatg
     1081 gagccaataa gtttctttac aaaatctgaa tggttcttgc ctctctacat tggttcagga
     1141 gtatggaaaa caataggttg gggttctatc atttatatgg gagcattaac caatatagat
     1201 tcaactttat atgaagcagc agatatggac ggggctaata gatggcacaa aatgtggcat
     1261 gtaactctac caagtttaag acctacagta gttactttgt ttatactaaa tgctggtggt
     1321 atcatgcaag taggatttga aaaggtattc ttgatgtcta gtcctgcaac atatgaagta
     1381 tctgatgtat tatctactta tgtttataga caaggtattt taaattctga tttcagttac
     1441 tcagctgcag taggattatt taataacata gtagctttgt tatttgtttt attagctaat
     1501 aagattgcta aaaaacttgg cgaagaagga attgtataag gggggtaaaa tga
//
LOCUS       DAVLTM010000202         1535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLTM010000202 DAVLTM010000000
VERSION     DAVLTM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.203"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..194)
                     /locus_tag="VYN16_002091"
     CDS             complement(<1..194)
                     /locus_tag="VYN16_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404538.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINS"
     gene            complement(191..523)
                     /locus_tag="VYN16_002092"
     CDS             complement(191..523)
                     /locus_tag="VYN16_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1404539.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(579..1481)
                     /locus_tag="VYN16_002093"
     CDS             complement(579..1481)
                     /locus_tag="VYN16_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404540.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWNIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIIINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
BASE COUNT          548 a          270 c          205 g          512 t
ORIGIN      
        1 gaatttatgg ctagacagat agatactatt ttatttttca cagaatatag aggtagtgat
       61 actattgagt agataaaagc taaaaaaata agcaaaaaat actccgtgtc atttataatc
      121 gccctgaatg ataaaaatat atacatccaa catacgataa tgtgtatttt tcgaatctta
      181 tctacattac tcatcttact gtcgcagata tgaatccatc ccaaggataa gggttcccgc
      241 taatatcaca acctctatta agagcaccat atccaataca gtaagctatt tttcctgctg
      301 ctgcaccaat aagccaacct ccaaggcctc caactttaat atagtttatt agttttttgg
      361 ttaaaactgc agctccagct aatgctataa aagttcctgg actcatagct aaagctgcgc
      421 aaattccttg acaagcacgc ttgcttaaat aagctccccc atcaagatac atcatttctt
      481 cttcatcaat cacaacataa ttatttggta atacaagttc catttgtttt ctcctcctta
      541 gttgaaattg tttcatttgt tttacaaata ggatgaactt aaactatctt tttattactc
      601 agtaataatt tccgagattt cattttatga tactctctat tcatacagaa atcaaaattt
      661 tgccttaacc aacttgtata atagaaaaaa tctaattgat ttattatgat gagctgtatt
      721 ctaattttat caaccttttc ttgccagttg ttattgttac taatagaaat ctcatacagc
      781 agtaactcga caagacctgt acacagaagg gtgttatgag aatcttcaat tttactcaga
      841 atatcctcaa agagttcact atccgttaaa aaggtggatt ggttcatcgc atcatacagc
      901 tgaagagaca ttaagtcaga ataactgaga tctgattttt gtattttatc ctttaacgta
      961 tttgtcaaat cactttcaaa cgaatcttct ttttcccatt tattttgatt gaaaagaaat
     1021 tccgtcttat taaaaacgag atgattgata tccattatat tccaaaaaaa tattgtagta
     1081 agcataatga tgacaaaagg aatttttaaa ataaatacta ggataatagc aatttcaaga
     1141 ataaaactcc atataatcct agatcttaaa tcatcaggcg acaaactttt acgatggaat
     1201 gaaaaatagg gcgagaacct cacatttact ttcttcctgc ctataaaaat aaaaggaaaa
     1261 atcgtaataa gagcttcttt cttgtacaaa tctttcaatg cgtatagtat gtctaaaact
     1321 atttttgata aaatcaaact acagaaagca agactcatac taatcacaaa atcaagggga
     1381 gattctagat tcaatagtaa gttattcact aggacatgca actctagcca caagacaaaa
     1441 agagggagca ttaataaaaa aatgattagt ttacgtatca taattttaca gtgtccttat
     1501 cattttctta attttggata aattatcatt gctat
//
LOCUS       DAVLTM010000203         1518 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLTM010000203 DAVLTM010000000
VERSION     DAVLTM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.204"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            159..1361
                     /gene="rpsA"
                     /locus_tag="VYN16_002094"
     CDS             159..1361
                     /gene="rpsA"
                     /locus_tag="VYN16_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1404541.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT          474 a          238 c          351 g          455 t
ORIGIN      
        1 ttaggtaata atcaagatag aaagcaaaat atcttagggt attttatttt tataattgaa
       61 agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc
      121 aagtttaaga aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc
      181 taaatagcgt tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg
      241 atgcgactca agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc
      301 gcgaattgac aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat
      361 tggatgttct tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg
      421 tatctaaaaa acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag
      481 aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag
      541 gtgttcgtgg atttatccca gcttcaatgt tggatactcg tttcgtacgt aacgctgagc
      601 gttttgtagg tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct
      661 tcatcctttc acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat
      721 tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg
      781 gcgctttcat cgaccttggt ggtgttgacg gattggttca cttgactgaa ttgtcacatg
      841 aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc
      901 ttgatcttaa cgaagaagaa ggacgtgtat cactttcact taaagcaaca gtaccaggac
      961 catgggatgg cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac
     1021 gtttgactga cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg
     1081 tatcacaaat ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag
     1141 aagttcaagt taaagttctt gaagttaacg cagatgcaga acgcgtgtca ctttctatta
     1201 aagctcttga agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc
     1261 gtccacgtcg tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag
     1321 gattttcaat ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa
     1381 aatcctttgt ttactaaaca agggattttt ctggctcttt gtcaactgta gtgggttgaa
     1441 gtcagctaag cttgagaaag gacaaatttc gtcctttctt ttttgatgtt caaagcgata
     1501 aaaatccgtt ttttgaag
//
LOCUS       DAVLTM010000204         1483 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLTM010000204 DAVLTM010000000
VERSION     DAVLTM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.205"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            36..1031
                     /locus_tag="VYN16_002095"
     CDS             36..1031
                     /locus_tag="VYN16_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404542.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            1021..>1483
                     /locus_tag="VYN16_002096"
     CDS             1021..>1483
                     /locus_tag="VYN16_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404543.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFQFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
BASE COUNT          507 a          205 c          283 g          488 t
ORIGIN      
        1 aaaatataat aaaaatgata gaataaggaa aaaacatgaa tgtcaaaaag ataatgtcaa
       61 tttttcaatc cttttatgtt gatgtcagta ttgaggaact gactttgact ttaccaatca
      121 gttttgtaaa aaggtttgag tatactcaaa tgacttttca taaggaatca tttttattga
      181 ttaaagaaaa gagaaggggg agtttgagtt catttgttac tcaggctcgc actatgggtg
      241 aaaaagccaa tatggatgtt gttttggtgt tttcgaagtt atcagacagt gaaaaaaagc
      301 aattacttca agctagagtt ccgtttgtag actttaaggg aaacctcttc ttccctccat
      361 tgggactagt actgaatgcg aatgatactg aagtccctaa ggaattaaca cctagcgaac
      421 aattaacgtg gattgccttt ttattgacaa aaggtcaaaa agtagtagat gttgatttgc
      481 tttcacaagt cactggactt ccaaactcaa caatttatag gtgtttgagg acttttaaag
      541 ctttatattg gttaaacaag caaaataagc tttacacata tacggtgtca aagaaagaat
      601 tattcttaaa atccgtgtca tgtttattta atcccatcaa aaaacggatt ttattgccag
      661 atggcgatat aaagcagata aaatctgttt ctaatcttct atatggtggt gcttatgctt
      721 tgtcgcattc aactttttta gctgaaacgg atgaaaatat tagctatgtc atatggcaga
      781 gaaaattcaa tcagttatcc ttgccacttt ctcagcatgt tttaaaagga aagatgctag
      841 agatatggaa atatcgtcct tttgtatctg agttttggaa tgattttaaa aataatcatg
      901 ataaacaatt tgtagatccg atttctcttt atttgacctt aaaagatgat gatgacccac
      961 gtatagagga agagagtgaa gcactagaaa atatgatatt acagtatctg ggagaagatg
     1021 atgccagcta atacgaaagt tatttttcaa gaaatgtttg cggattttca gaactattat
     1081 gttctgattg ggggaactgc tacctctatc gtattggatt cgcaaggatt taaaagtcgc
     1141 acaacaaaag attatgatat ggtcatcatt gatgaagtaa aaaataagga attttatact
     1201 accttgaatc attttttaga attgggagag tatcaaggaa gtcagaaaga tgagaaagcg
     1261 cagctttttc aatttacaac aactaatcct gagtttcctt ctatgattga actatttagt
     1321 atcttaccag aatatccatt aaagaaggac ggtcgagaaa ttcccttata ttttgatcaa
     1381 gatgctagtt tatcagcctt attattggat gaagattatt ataatatatt ggtgcatgta
     1441 aaagaaacca ttcaggggta ttcggtattg agtaattgtg gtc
//
LOCUS       DAVLTM010000205         1440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLTM010000205 DAVLTM010000000
VERSION     DAVLTM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.206"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            208..440
                     /locus_tag="VYN16_002097"
                     /pseudo
     CDS             208..440
                     /locus_tag="VYN16_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982220.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            458..1216
                     /locus_tag="VYN16_002098"
     CDS             458..1216
                     /locus_tag="VYN16_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1404544.1"
                     /translation="MKKIKASTWNINQRSGQGKQIPEMIVHELAKQNSEIVCLTEYVK
                     TDNPSLFCTKLQDMGYKIFEDVRTIDFGNQILIAIKNDLVNDCQVITIDNDDNNPNFL
                     HVRVTIEGQELNIIGVRIKVGGENIFQDFKHRKSQLDKLISNLPATHENTIVLGDFNN
                     GYFQQNDDIHSYQGKAREFYSYPLLKSEMNKAGLTVHTPSEETSWKYCKLDHIFANIP
                     ISNENYSWEFLKNADYNSRVGFPDHAILSASISL"
     gene            1246..>1440
                     /locus_tag="VYN16_002099"
     CDS             1246..>1440
                     /locus_tag="VYN16_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404545.1"
                     /translation="MSDNLKAYIFLLGFLILAGIAIFILPQGHFFSVSALVLGCIPLF
                     ISIYYFAKIKLLGFYELLKEL"
BASE COUNT          516 a          236 c          225 g          463 t
ORIGIN      
        1 tctttgttgt tgattttttt atttaactgt agtataatac caaacatagg ggaatatccc
       61 acaaatatac tatacaaaaa ggaggattct tatatttaca gaacttggag aaatctatga
      121 tggatacaac ttctagcact tcctcatttc actcatacat cacacaggag catttgctcc
      181 ctcaattaac attaaagaga ggacaaaatg atgaatacat taaaaactga acaatatcaa
      241 gaagcacgag atagattaga tactcttgat accgtttttg cactcagccc tagtctcact
      301 aatcaatggg aaaaatagac actgataaag cacattatag agagctagta aatgagtatg
      361 aaaccagaac aggaaatctt gtttaccttg ttattgcaaa caatcatcta ataaatcttc
      421 tttacgttag taaagcagaa catagaaagg tttatatatg aaaaaaatta aagcatccac
      481 ttggaatatc aatcaacgtt caggacaagg aaaacaaatt cctgaaatga ttgtacatga
      541 attagctaaa caaaattcag aaatcgtctg cttgacagaa tatgtaaaaa cagataatcc
      601 tagtttattc tgtactaaat tacaggatat gggatacaaa atttttgaag atgtgcgtac
      661 tattgatttt ggaaatcaaa tccttattgc aataaagaat gatttagtta atgattgcca
      721 agtcattact atcgataatg atgataataa tccaaacttt ttacacgtta gagtgacaat
      781 tgaaggacaa gaattaaaca ttattggagt tcgtatcaag gtcggtggag aaaacatttt
      841 tcaagatttc aaacatcgaa aatcgcaact agacaaacta atctcaaatt tacccgcaac
      901 acatgaaaat acaattgtgc tgggtgactt taataatgga tatttccaac agaatgacga
      961 tattcattcc tatcagggca aagctaggga gttttactct tatccattgc ttaagtctga
     1021 aatgaataaa gcaggcctaa ccgtacatac tccctctgag gaaacctctt ggaaatattg
     1081 taaattagac catatttttg caaacattcc tatatcaaat gaaaattatt cttgggaatt
     1141 cttaaaaaat gctgattata atagccgagt cggctttcca gaccatgcta ttctatcagc
     1201 ctctatctct ttatagtact tgtttattag aggaggtaat gcaaaatgag tgataattta
     1261 aaagcatata tatttctttt aggatttctg atattagctg gcatagcaat ttttattctt
     1321 cctcaagggc atttcttttc agtttctgcc cttgttcttg gatgtattcc attatttatt
     1381 tctatctatt atttcgcaaa aatcaagctc ttagggtttt acgaactttt aaaagagctt
//
LOCUS       DAVLTM010000206         1440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLTM010000206 DAVLTM010000000
VERSION     DAVLTM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.207"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            82..1260
                     /locus_tag="VYN16_002100"
     CDS             82..1260
                     /locus_tag="VYN16_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1404546.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          535 a          262 c          206 g          437 t
ORIGIN      
        1 ttaaaaagat taacatctac atttatccat agcaaaaaat cataggattt taagaatctg
       61 acaactagga agaaaactaa tatgacctta tccaagaaac aattacaact aagagctaaa
      121 atactggaaa cagtttacac attaggtccc atttcacgaa ttgaaatcgc tactaaaaca
      181 ggaataacac ctgcaacaac tagcagtatc acaaatgatc tgattaaaga aaatattctc
      241 ttagaacttg gtgaagacga acacgataca agcgtaggga gaaaaaaaat cctgctagac
      301 attcaagcta aacgatttta ctacattggt tgtgaactat ctgaaaaaca tttcactttt
      361 gctttaggag ataatctagg aaatatctta aaggaagaga aagaaattgt tactaaacag
      421 ttaatccaag aaaagggaaa tcaactcatt aaccagacac taaagcaatt cttaaataac
      481 tgctctgatt atgaaataga agcaattgga atcgcgctac ctggtcgcta tttagataat
      541 tataaaataa caacaaacaa tcctttatgg caacatatcg acttagaaat gattcaatcc
      601 cactttgata agcctttgtt tttctctaac aatgttaact gtatggctat aggtaaacgc
      661 ctatttagtc gccaacagaa tgaccctaat tttgcttatt ttcattttgc tagaggtatg
      721 cactgttctt atatctacga cggaaacatt tatgggaaag gaaatctaat gattggagaa
      781 atagggcata cagttgtttc atctgaggga gaagaatgta gctgcggacg caagggatgc
      841 ttacaaactt ttgctggaga atcttggtta atcaaaaaat caaaaattct atatcatcaa
      901 tctccctatt ctctacttcc tagtttagtc aaaaatgccg atgatattga tatccaagtt
      961 attttaaccg cttatcaatt aggtgacact ggaataatca cacttatcca tcaagcctta
     1021 ctctatctta gtcaaaccat tttaaatatc agtatgatga ttgactctca aaaaatctac
     1081 ctacatagtc ctttactaac aaatcaacac atcatccaaa aactatatag tgaaatgaac
     1141 tacaaaccca aactgctcta taatcgccta cctgaagtca ttattgaacc ttacaatgat
     1201 tttacagccg cacactcagc tattgcactt tgtttgtatc atacaattct acattcttaa
     1261 ttttctatta catttaataa ataggctaaa aagagggagt atatcataaa aagcgaataa
     1321 acctgttttc tgaaaatcag aattctgttt tgttcgcttt ctttatattt ggaattatac
     1381 tcctatctcc cctttttcat aatgtataat aaaatgaaat aataacagga cgaatcgatc
//
LOCUS       DAVLTM010000207         1435 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLTM010000207 DAVLTM010000000
VERSION     DAVLTM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.208"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            18..1409
                     /locus_tag="VYN16_002101"
     CDS             18..1409
                     /locus_tag="VYN16_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1404547.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT          346 a          270 c          316 g          503 t
ORIGIN      
        1 ttattttgga gtcaaagatg aatatattta gaacaaagaa tgttagttta gataaaacag
       61 agatgcatag gcatttgaag ttatgggatt tgattttgct gggtatcgga gccatggtag
      121 ggacaggcgt ctttacaatc acaggtactg cagctgcaac acttgctggc ccagccctag
      181 tgatttcaat cgttatttct gccttgtgtg tgggattatc agccctcttt tttgcagaat
      241 ttgcctcgcg agtacccgct acaggaggtg cctatagtta cctctatgct atcttaggag
      301 aattccctgc ctggttggct ggttggttaa ccatgatgga gttcatgaca gccatatcag
      361 gcgtagcttc gggttgggca gcttatttta aaggcttgct ctctcaatac gggatagcct
      421 ttcctcaggc tttaaatggt acctttaatc ctcaagcagg gacatttgtt gatttattgc
      481 ctattcttgt cttggtcttg gtgacttcgc ttgttttact aaatgccaag gcagccttac
      541 gctttaattc gattctagtt attttgaaat tttccgcttt agctctcttt gttttagtag
      601 gaatttggaa tattaagttt gataattgga gcaattttgc tccttacggt tttggacaaa
      661 tctatggagc tagcactggt attatggctg gtgcgtcctt gatgttcttc ggttttttgg
      721 gatttgaatc catctctatg gctgtagatg aagtcaagac tcctcaaaaa aatattccta
      781 gagggattgt cttatcgctt tctatcgtaa ccattctcta tgccttggtg acccttgttt
      841 tgactggtgt ggttcactat agtcatttaa atgtcgatga tgccgttgcc tttgcccttc
      901 gtagtgttgg gattagttgg gcagccaact atgtgtcatt agtggctatc ttgaccttga
      961 ttacagtttg tatttcgatg acctatgccc tatcgcgtat gatttacagt ttagcacgtg
     1021 acggcttagt gcctgctgcc tttaaggaac tgacgaagac tagcaagata ccaaagaatg
     1081 ctactatctt gacaggtcta gcttcagcag tagcagcagg aatgttccca ctagccagta
     1141 ttgcagcctt tttaaatatt tgtaccttag cctacttgat catgttggct tatggtctga
     1201 ttcgcttacg gaaagaaaaa ggaatgccca aagctgggga atttaaaaca ccattggtac
     1261 ctctgttacc aattttatcg atcatcattt gtttatcctt catgttacag tataatatga
     1321 atacctggat tgctttccta gttgcgttgt tagtaggaag tattatttac ttcacttatg
     1381 gctataagca ttctaccata gaagaataaa gtgaaagtct attgagttgt tgaca
//
LOCUS       DAVLTM010000208         1406 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLTM010000208 DAVLTM010000000
VERSION     DAVLTM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.209"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            17..1363
                     /gene="gdhA"
                     /locus_tag="VYN16_002102"
     CDS             17..1363
                     /gene="gdhA"
                     /locus_tag="VYN16_002102"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1404548.1"
                     /translation="MTSAKEYIQSVFETVKTRNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          416 a          284 c          306 g          400 t
ORIGIN      
        1 ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt tcgaaactgt
       61 aaaaactcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat ttttcaacac
      121 tttggaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct tggcacgtat
      181 tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg atggaaaaat
      241 tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat acaaaggtgg
      301 acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat ttgaacaaat
      361 ctttaaaaat gtcttaactg gacttcctat cggtggaggt aaaggtggat cagacttcga
      421 tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca tgactgaatt
      481 gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg ttggtggacg
      541 tgagattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg ctggtgtctt
      601 gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa ctggttacgg
      661 tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg ctggtaagaa
      721 agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag caactgaact
      781 cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg aaaatggtat
      841 cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga ctgagtatgc
      901 agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggacttacg ctggaaacta
      961 tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag ctaaacgttt
     1021 ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg accttgatgc
     1081 catcaaagtt tacaaagaaa atggtatctt atacggacct gcaaaagctg ccaacgctgg
     1141 tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct catggactcg
     1201 tgaagaagtt gatgaccgtc tcaaagacat catgacaaac atcttcaaca cagctaaaac
     1261 aacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca ttgctgcctt
     1321 tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt taagatttat ttactcaatc
     1381 ctcaatcact acgattgggg attttt
//
LOCUS       DAVLTM010000209         1398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLTM010000209 DAVLTM010000000
VERSION     DAVLTM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.210"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            88..1266
                     /locus_tag="VYN16_002103"
     CDS             88..1266
                     /locus_tag="VYN16_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404549.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDCQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT          477 a          273 c          293 g          355 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagctgact gcgactagct ttctagtttg ctctttgatt
       61 tttattgagt attagaaagg agacctcatg acacttccag ttagaaaatc cctgcacgat
      121 gcagttttac aggcttcgaa agcggatact tgggaacaag ctaccaagga atggaatgaa
      181 gtttccttga tttttaacgg tatcggccgt agcaattgtg tctgtggaaa tgccatcaaa
      241 tacgcttatg aactctttaa cggtgtcaca ggccaacgcc tctttccaat tggtagcgac
      301 tgtgttcgtc attttcatcg attgtccctt gaccagcaat tggaagagga agaaaaactg
      361 ctcagaaagg ttgaaaatct aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat
      421 aaaagtgact ttgacgagcg acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc
      481 aatcgtggca atcaatttgc gcctgagaga gactgccagc ttttcctaga agtctttcag
      541 ggaggaagtt ggaccaaggc ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa
      601 aagtgtatca aacccttttt acttgggaaa tcggatgacc aactctacct tgtcaagcta
      661 ggcaaggaga aaattgacta cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag
      721 aagagggata aaatcgccaa gcaatatgca gacaatctgg ttctagccat gggacccgca
      781 gagcgtgctt atcaagatta ctttggcttt acagagaccc tgactcaaga agaacgcaag
      841 tgggaaaaaa ttctttttgg taaaaataga gcagaacggg ctatcaaggc caaacaatac
      901 caaaaagagc tggaaaaaga ccaacgaatt gccagtcagg atccaattga aagaaaacag
      961 aagcagactt ggctcctcaa ttcctatttt cgtgagcttc ctgaagaaaa agctcgattt
     1021 tctaggctct tattagaata tcgaaaaaat ggtgaagtac ccttttcaac tgaatacctg
     1081 tcagatcatc tcatcgactt tttctataaa atgaaagcct ttgaatttga aatcgcacca
     1141 gaacaagtcc gagattttct aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa
     1201 ggaagctgga tagaaggaat tctcctcaac tgcctcaaac catttttaga acgattagtg
     1261 atatgagtaa cacctaccgt gtaaatttca tggtaggtgt tattgtacct caaaataata
     1321 taaaacaaag tatgaaagcg catctcaatt ttaagacttt tttgtaaatt aagagtataa
     1381 ttaaggtata ctgccttt
//
LOCUS       DAVLTM010000210         1359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLTM010000210 DAVLTM010000000
VERSION     DAVLTM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.211"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            19..936
                     /gene="adcAII"
                     /locus_tag="VYN16_002104"
     CDS             19..936
                     /gene="adcAII"
                     /locus_tag="VYN16_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1404550.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSILAEELK"
     gene            944..>1359
                     /locus_tag="VYN16_002105"
     CDS             944..>1359
                     /locus_tag="VYN16_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1404551.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYL"
BASE COUNT          463 a          230 c          298 g          368 t
ORIGIN      
        1 atggttaagg agttgttcat gaagaaacaa aatttatttt tagtcctgtt aagtgtcttt
       61 cttttgtgct tgggggcttg tggtcaaaag gaaagtcaga caggaaaggg gatgaaaatt
      121 gtgaccagtt tttatcctat ctacgctatg gttaaggaag tatctggtga cttgaatgat
      181 gttcggatga ttcagtcaag tagtggtatt cactcctttg aaccttcggc aaatgatatc
      241 gcagccatct atgatgcaga tgtctttgtt taccattctc atacactcga atcttgggca
      301 ggaagtctgg atccaaatct aaaaaaatcc aaagtgaagg tcttagaggc ttctgaggga
      361 atgaccttgg aacgtgtccc tggactagag gatgtggaag caggggatgg agttgatgaa
      421 aaaacgctct atgaccctca cacatggcta gatcctgaaa aagctggaga agaagcccaa
      481 attatcgctg ataaactttc agaggtggat agtgagcata aagagactta tcaaaaaaat
      541 gcgcaagcct ttatcaaaaa agctcaggaa ttgactaaga aattccaacc aaaatttgaa
      601 aaagcgactc agaaaacatt tgtaacacaa catacagcct tttcttatct agcgaagaga
      661 tttgggctta atcaacttgg tattgcaggc atctctcctg aacaagaacc aagtccacga
      721 caattaacag aaattcagga atttgttaaa acctataagg ttaaaacgat ttttacagaa
      781 agtaatgctt cttcaaaagt agctgaaact cttgtcaaat caacaggtgt gggtcttaaa
      841 actctgaatc ctttagaggc agacccacaa aatgacaaga cctatttaga aaatctggaa
      901 gaaaatatga gtattctagc agaagaatta aagtgaggaa agaatgaaaa tcaataaaaa
      961 atatctagca ggttcagtgg cagtccttgc cctaagtgtt tgttcctatg aacttggtcg
     1021 tcaccaagct ggtcaggtta agaaagagtc taatcgagtt tcttatatag atggtgatca
     1081 ggctggtcaa aaggcagaaa atttgacacc agatgaagtc agtaagagag aggggatcaa
     1141 cgccgaacaa atcgtcatca agattacgga tcaaggttat gtgacctctc atggagacca
     1201 ttatcattac tataatggca aggttcctta tgatgccatc atcagtgaag agctcctcat
     1261 gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca agggtggtta
     1321 tgtcattaag gtaaacggta aatactatgt ttaccttaa
//
LOCUS       DAVLTM010000211         1344 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLTM010000211 DAVLTM010000000
VERSION     DAVLTM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.212"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..663
                     /locus_tag="VYN16_002106"
                     /pseudo
     CDS             1..663
                     /locus_tag="VYN16_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            813..1067
                     /gene="blpM"
                     /locus_tag="VYN16_002107"
     CDS             813..1067
                     /gene="blpM"
                     /locus_tag="VYN16_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1404552.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            1083..1286
                     /gene="blpN"
                     /locus_tag="VYN16_002108"
     CDS             1083..1286
                     /gene="blpN"
                     /locus_tag="VYN16_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1404553.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          449 a          204 c          309 g          382 t
ORIGIN      
        1 attaagccta gaagaccttc ttatggccac tcttcaatat gtgcgagaat accgcactta
       61 tgaagaaatt gcggctgatt ttggtatcca cgaaagcaac ttaatccgtc ggagccaatg
      121 ggttgaagta actcttgttc aaagtggtgt tacgatttca aaaactcatc ttagtgctga
      181 ggatacggtg attgtggatg caacagaggt aaaaataaat cgtcctaaaa aaatcaacta
      241 gccaattatt ctggtaaaaa gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt
      301 caagggagaa ttgtttcttt ggatatcact gtgaactatt gccacgatat gaagttgttc
      361 aaaatgagtc gcagaaatat cggacaaact ggtaaaacct tggctgacag taattatcaa
      421 gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa actcaaaccg
      481 ctaacagtta aagataaagc ctataaccat gcgttatcta aggagagaag caaggttgag
      541 agcatccttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
      601 aaacgcttcg gattacgaat gaatttgatt gctggcatta tcaatcatga actaggattc
      661 tagttttgca ggaagtctaa tgtaattttt gcaattcaag acgtttcgat gactattcaa
      721 gatctggatg aaaaatttta aaaaacgatg atatactaaa cttgtcaaag ttacaacaag
      781 acaaaaatta aaaataaaaa agagtatttg tcatggatac aaaaattatg gaacaatttc
      841 atgaaatgga tataactatg ttatctagta ttgaaggagg caagaataat tggcaaacta
      901 atgtcttaga aggtggtggt gctgcttttg gtggctgggg gctagggaca gctatttgtg
      961 ctgcgagtgg tgttggagca ccatttatgg gagcatgtgg atacatagga gctaaatttg
     1021 gtgtggatct ttgggcagga gtaacagggg caacgggtgg attttaataa aaggagactt
     1081 gtatgaatac ttattgtaat ataaatgaaa caatgctgag tgaggtttat ggaggtaatt
     1141 ccggaggagc agctgtagtt gctgctttag gttgtgcagc gggtggagtg aaatatggga
     1201 gacttctagg accatggggc gctgcaatag gaggaattgg aggagcagtg gtttgtggat
     1261 atttagccta taccgctaca tcataaaaat gaaagctaaa ttttatgaag gctatattct
     1321 ttattattct cttcgctttt caaa
//
LOCUS       DAVLTM010000212         1331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLTM010000212 DAVLTM010000000
VERSION     DAVLTM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.213"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(135..>1331)
                     /locus_tag="VYN16_002109"
     CDS             complement(135..>1331)
                     /locus_tag="VYN16_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1404554.1"
                     /translation="MGFNVFRTSIAWSRIFPKGDEEEPNEAGLKYYDELFDELHAHGI
                     EPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYKDKVKYWLTFNEVNSV
                     LELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREINSEFKVGCMVLAMPA
                     YPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEHDINIEFAAEDAELLK
                     NYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESSEWGWQIDPIGLRIIL
                     NRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYMEEHLLQVAEALQDGV
                     EIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRYKKMSFTWYKGVIESN
                     GESLFK"
BASE COUNT          442 a          260 c          171 g          458 t
ORIGIN      
        1 tgtaacaaaa ttaaattaat taatccccct ataaaataaa gaggtttaga atttgatgtc
       61 ttccaaacct ctttatttca tatttaatga gatgttgcct taaccgtgat aatatctaat
      121 catcaataaa caaactattt gaataaggat tctccatttg attcaatcac tcctttatac
      181 caagtaaagg acattttctt atatcgattg aatgtgccat ttccatcatc atttcgatca
      241 acataaatga gaccgtacct tttagaaagt tgtgcagtgg acatagaaac gcagtcaata
      301 catccccaag atgtatagcc cataatttcc acaccatctt gtagagcttc agctacttgc
      361 aataaatgtt cttccatata ctgaattcta taatcatctt ggactgttaa gttattaaat
      421 tcatctttta ttagttgatc tttagcacct aatccgtttt ccactataaa taatggaatt
      481 tgataacggt cataatatct atttaaaatt atacgtagtc caattggatc aatttgccat
      541 ccccactcag aagactctaa ataaggattt actaagccac caataatatt cccttctcct
      601 gaattatact gtgttggaag agcagattga gtcacactca tgtaatagct aaaggataaa
      661 aaatctacag tataattttt taataactct gcatcttcag ctgcaaactc tatgttaatg
      721 tcatgttcct taaaatatct ttttgcataa ttcggataat aacctctaac atgcacatct
      781 gaaaatagat aatttagatt ctcatactca tgagtcgccc atacatcttt tggatttgga
      841 gtcattggat aagctggcat agctaatacc atacacccca ccttaaactc tgaattaatc
      901 tcacgagcaa tttttgtaac caaactcgag gcgactaatt catgatgtat agcttgatat
      961 aattcttgtt tcgaaagatt ctccttaggt atatctattc ctccactagt aaacggtaat
     1021 tccaaaacag agtttacttc gttaaatgta agccaatatt taactttatc tttatacctt
     1081 tctaaaactg ttcgagcaaa tttttcataa aaatgaatca ttctcctatc aatccatcca
     1141 tgatattttc ttgctaaata taatggagtc tcatagtgtg aaagagttac aagtggttct
     1201 atcccgtgag catgtagttc atcaaataat tcatcataat atttcaaccc agcttcgtta
     1261 ggttcttctt catctccttt tggaaaaatt ctactccatg caatagaagt acgaaaaaca
     1321 ttaaagccca t
//
LOCUS       DAVLTM010000213         1257 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLTM010000213 DAVLTM010000000
VERSION     DAVLTM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.214"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..291)
                     /locus_tag="VYN16_002110"
     CDS             complement(<1..291)
                     /locus_tag="VYN16_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404555.1"
                     /translation="MSEVDFNEAVTYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDF"
     gene            complement(349..1062)
                     /locus_tag="VYN16_002111"
     CDS             complement(349..1062)
                     /locus_tag="VYN16_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1404556.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAEFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          406 a          243 c          212 g          396 t
ORIGIN      
        1 aaaatctccc atacagccat tacgattcat attgaggcta tattttcgat tagtaagata
       61 attcggaaat atatttccta ttactctaag attatctact tgtgaatccc ttctcccaat
      121 ataatcccct tctttgacta atgatggaga tttccaagta aaaattaaat cgccagagaa
      181 atttttctta tcaaaaaact taaaaagaga ttgataaata ctatttgcta attgacaagt
      241 gtcagatgta aattcataag taacagcttc attaaaatca acttcagaca tttcaatgta
      301 ctcttcaata gtaatattag cttttttgta gtcattagct cgtctccttt aaaatgtgat
      361 cttttcaaga atatttttat ctaaacaatc cagcaagtct tggtaagaat agacttcgta
      421 agtcggctgg gcttgtgtgt gattttcgag gtgatgagga ttataccaga tagtgtcaat
      481 ccccgcatta ttgccacctt gaatgtcggc agttagagaa tctccaatca tcagcgtctt
      541 ttctttacta aattcagcaa tttgctggcc aatcttttca taaaagagag catccggctt
      601 ttgagtttgc aactgttctg agataaagac ttgattgaaa taaggtgcta gaccagattg
      661 agccaaacgt cctgtctgaa tggcagtaat gccatttgtc gcagcataca agttataatc
      721 acgctcaatg aggctgtcca agagatcatg agcgcccgat agtgtttgtc cctgctgggc
      781 gaggtaaaat tggtaacgct gggcaagaaa actaccgtct ttttcctgtc caaaatgagc
      841 aaataaacga gaaaagcgcg tgttaaccag ctcttgttta ctgattttct tcagctccaa
      901 gtctttccag agagccttgt tcataggaac gtaataatct ttataagcct gaatatccgc
      961 aactccttct tcttttagaa gttgggtcaa agccacatcc tcagcagcat caaaatcaag
     1021 aagagtgtgg tcgaggtcga agagtagaaa tttgtagaac aatttgaggt tttcctttct
     1081 gaaaattcat taagaacatt atatcataaa gcacctcata caattaacta atttaatcac
     1141 ttaaaaaaaa ttcgaacact ttctatacaa ctgacagctc aaatctttca gaatagaaca
     1201 atactaacta tcgagcaccc cgtcttcata aatatatgtg taattctaga cctagaa
//
LOCUS       DAVLTM010000214         1205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLTM010000214 DAVLTM010000000
VERSION     DAVLTM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.215"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            26..892
                     /locus_tag="VYN16_002112"
     CDS             26..892
                     /locus_tag="VYN16_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1404557.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
BASE COUNT          464 a          143 c          232 g          366 t
ORIGIN      
        1 tcaaatagaa aaggaagatg caaacgtggg tacactatta gcaacaagat taaaaaatag
       61 acgaaaagaa ttaaaaatgt ctcagcgaga attagctgag ggtatctgta aacaggggca
      121 aattagtcga ttagagagtg gagagttcac ccctggagcg gattttttac atgcccttgc
      181 taagaaatta aaagttagta tggactactt ttttgatgag caggttgtag aggaagtcaa
      241 tgagttatca gagtttaaga agttagcaca gacatttatc acaaatcgta attatgaatc
      301 tttaaaatat atatatgaat tggaaaatgt aaaagcccat cgcttatctc tagcagataa
      361 attttacatg gaatggataa agtctcttat agatttttat ttttatggac agaaagaaga
      421 ggcaattaca cgattagaga atatgctacc tcagttaaat gtatctgatt tgaactacct
      481 tcaagtttca aacactctat tcaattttta ttatgatatt gaagatttag aacgttttaa
      541 tgagattaga gacaagctag agcatcaagt gaatcagctt aatttaaaca caattgaaga
      601 gttagaacta tcaattaagt tcaattataa cgtttgtcga tacctatggt tacagaaaaa
      661 tattgaagaa gctattacga aaatcacagc tacgataaag caatgtaagg agtacaggac
      721 aacttatcta ttagctgatt tgtatttatt gatgggaagt gtaagtgaaa atttttcttc
      781 taaaagttca gtaaaagaat attttgagac tgctcatttt ctctacaaac ttgaggaaaa
      841 catgtcaatg gctttgaaag tcgaacatta ttttgcagat ataacagaat aaggaatacg
      901 gtgttctagt gagcatcgtt ttttaaatct aatttgaata caaaaaagta ttaaataaaa
      961 aaaaataaaa aaagattata aaaatacttg acaagctaat tttgaagtga taaaataaat
     1021 gcaagaaaac gaaaggagag gtatatgaga agtttgttta ggaaaattgt tgctttgtta
     1081 gtcataggat taatcctatt agggactgct ggaggaactc aagttcataa gatggctaga
     1141 gggattgatc caggtcctgc gaatggtatt tatagatagg agattatagt cacttaaaaa
     1201 tcaaa
//
LOCUS       DAVLTM010000215         1190 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLTM010000215 DAVLTM010000000
VERSION     DAVLTM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.216"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(149..817)
                     /locus_tag="VYN16_002113"
     CDS             complement(149..817)
                     /locus_tag="VYN16_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1404558.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(1032..1120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          391 a          255 c          198 g          346 t
ORIGIN      
        1 ttgctcaaaa cactgctttg agcctgtaga taagactgac gaagtcagct caaaacactg
       61 ttttgaggtt gtggatagaa ctgacgtggt ttgaagagat tttcgaagag tcttacctca
      121 tcaaatttgt aaatatcatg agccttctct agacatcgta accaatatca aaaaaagcta
      181 attctaaagc gactgcttga ttccagcgtt gctgaagttc tgtcaaatct tctcgatttt
      241 taccgacacg attgagttcg tcaaccagaa attgaaccca ctctgcaaag aaaggacctc
      301 tgtggagatt gatccattcc gaatgaatat agacttcagg taaagccaaa tctttagaac
      361 cccagtctaa atagagacct tctgcaatga ccagcatgac caaaagatgg gcatagtctg
      421 atgaagccac cgccgaatac attagatcct gaaaggcttt tgttacaggg tgcaaagtca
      481 cttctagata gtcattctct gctactttta actctttaaa agccttttgg aaataaccat
      541 cttcatctgc ttcaagaaag cctagttgct tggcaaaacg aagcttggat tcaagtttat
      601 ctgcgtgagc tacgcaggca cccagcatgg ataagaaggc atcaaagaag tgataatctt
      661 gaatcagata gtcctttaag accttattct caattgtccc cgcaaaaagt tccttaacaa
      721 aacgatgatt gattgcagcc tgccaatcct tctgactgct ttttaataat tctccaacag
      781 tcaaacctgg ctgaaatgca tagtcttgtg tttccatatt tacttctcct ctctttactt
      841 gttagtaatt aataaaacac caagaaatat caagcaaaat cgtaattcca cttgatcctt
      901 ttaaaacaca tcgagagtat ttgcagagag ctaactaaac aagcctatcc agtttatata
      961 aacaaaaaac tccaattaca atcaagaatt agagttgact tacaagatta gatcgttcat
     1021 ttcgccatac gaaaaaacgt ttcacatttc ccttcgccag tcttaactgt atcaggttca
     1081 atgggtatca tctcagctta aagcacccca aatgtctcta ttatttaatt actgaaccag
     1141 tatagcaaaa aatgaaagcc ctagcaagat atttgaccga aaaatatctt
//
LOCUS       DAVLTM010000216         1123 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLTM010000216 DAVLTM010000000
VERSION     DAVLTM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.217"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..613)
                     /locus_tag="VYN16_002114"
     CDS             complement(<1..613)
                     /locus_tag="VYN16_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1404559.1"
                     /translation="MKKVSIILPVYNVEQYIKKCLESIQQQTYPNLEVIIVNDGATDK
                     SVEYCEQICKIDSRFSVTHKENGGLSDARNVGIDKAKGDYLIFVDSDDFVSQDMVSYL
                     VSSMENNEADIAICDPAHYYSDRQNNDLNIFYPASSVKVYEKTEALCEMFYQKSFLVS
                     AWAKIYKKELFDDIRFPVGKLFEDSAVMYLLFEKCEKIVYSNAK"
     gene            complement(623..>1123)
                     /locus_tag="VYN16_002115"
     CDS             complement(623..>1123)
                     /locus_tag="VYN16_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404560.1"
                     /translation="RPVIITTWLVISLLSIIFFFKHIINNSESYTHRVLGIVNFFKYY
                     ESSKFHLFFGDAELAFGDMTKGYTHNIRSVLGWDGTVEMPLLSVMIKNGYVGLIGYGV
                     VLFKFISSVLSMEDRRVKNIGLSILIPLLLSAMVENYIVNISFVFMPVCFCILCSIKN
                     IEFKNN"
BASE COUNT          405 a          205 c          138 g          375 t
ORIGIN      
        1 atttcgcatt agaatatact atcttttcac atttttcaaa aagcagatac ataacagcac
       61 tatcttcaaa taacttccca acagggaatc tgatatcatc aaatagttcc tttttatata
      121 tcttagccca agcagatacc aaaaaagatt tttgataaaa catctcacat agagcttccg
      181 ttttttcgta aaccttaacg cttgatgccg gataaaaaat attaagatca ttgttttgac
      241 ggtcactata ataatgagca ggatcgcaaa tagcaatgtc tgcctcattg ttctccatac
      301 tagatactaa ataagatacc atatcttgag agacaaaatc atcactgtca acaaaaatga
      361 ggtaatctcc cttggcttta tctataccga catttcttgc atcggaaagt ccaccatttt
      421 ctttgtgagt gacagaaaac ctagaatcta tcttacaaat ttgctcacag tattcaacgc
      481 ttttatcggt tgctccgtca ttaacaatga ttacttccaa atttggataa gtttgttgtt
      541 gaattgattc taagcatttt tttatatact gttcaacatt gtagacaggt agaataatac
      601 ttactttttt cataaatcac tttcaattat ttttaaattc tatatttttt attgagcata
      661 aaatacaaaa acagaccggc atgaatacaa agctaatatt aacgatataa ttttcaacca
      721 ttgctgatag aagtagaggt attagaatgg ataagccaat attttttact cttctatctt
      781 ccatagataa tacagaggat ataaatttaa ataagactac tccataccct attaagccga
      841 cataaccatt ttttatcata acacttagca acggcatttc cacagttcca tcccaaccta
      901 aaacacttct tatattgtga gtataacctt tcgtcatatc accaaaggct aactcagcat
      961 caccaaaaaa taaatgaaat ttgctagatt cgtagtattt aaaaaaatta acaataccca
     1021 aaacacgatg agtataggat tcactattat ttataatgtg tttgaaaaaa aatattatag
     1081 ataataaaga aattactaac catgtcgtta taattactgg tct
//
LOCUS       DAVLTM010000217         1109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLTM010000217 DAVLTM010000000
VERSION     DAVLTM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.218"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..>1109
                     /locus_tag="VYN16_002116"
     CDS             <1..>1109
                     /locus_tag="VYN16_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1404561.1"
                     /translation="VEVVNIKGKEIVIIPVNKVDYKDKPIYLNDNIASTYFRQGTGDF
                     RCSQEQINSMLRDSAKESFDSTLIQDFSILDLDTETIKLYREKFDNINAEHPFSKLDN
                     EQFLIKINALRRDRKDDKIKPTVAGLLIFGTHNSIKEFIPHYNVEYVLKEFSENNRFK
                     DRVIYDGTWGEDNLFNFFYLVIEKLYLTLNDNSNIQENSMNRIGISKLRIAIREAFIN
                     SLIHSDYKSEKGIMIIRYPDRYIFTNGGTLRIDIKDFFSGAHSDPRNYLIQEIFRFLN
                     LCEKAGTGIPKIMEAVKESHLKYPNLRTELDSVEFTLWDTSLIDNLDIDNEYEKKILE
                     LIIEHRFVTREALEEKLKIHKNTILKYLKKLVEKQ"
BASE COUNT          439 a          149 c          185 g          336 t
ORIGIN      
        1 gtgttgaagt cgtaaatata aaaggaaaag agattgtcat tatccctgtt aacaaggtag
       61 actacaagga taaacctata tatcttaatg acaacatcgc atctacctat tttcgtcagg
      121 ggactggtga ttttaggtgt tcacaagagc aaatcaactc tatgttaaga gattccgcta
      181 aggaaagttt tgatagcaca cttattcagg atttttctat tttagactta gatacagaaa
      241 caataaagct atatagagaa aaatttgata atataaatgc ggagcaccct ttttcaaagt
      301 tggataacga acaattttta ataaaaataa atgctttacg aagagatcgc aaggacgata
      361 aaataaagcc aacagttgct ggactattaa tttttggtac acataattca ataaaagagt
      421 ttattccaca ctataacgta gaatatgttc taaaagagtt ttctgaaaac aatcgcttta
      481 aagatcgtgt gatttatgat ggtacatggg gggaagataa tctttttaat ttcttttacc
      541 tagttattga aaaattatat ctaactttaa atgacaactc taacattcaa gaaaattcta
      601 tgaatagaat tggaatttca aaattacgaa ttgcaattcg tgaggcattc attaatagcc
      661 ttattcatag cgactataaa agcgaaaaag ggataatgat aattagatat cccgacaggt
      721 atatattcac aaatggcgga acacttagaa ttgacataaa ggactttttt agtggggcac
      781 attctgatcc aagaaactac ttaatacaag aaatttttag atttttaaat ttatgtgaaa
      841 aagcgggaac tggtataccc aaaataatgg aagcagttaa agagagtcat ttaaaatatc
      901 ccaatttacg aactgaatta gactcagttg aatttacatt atgggatact tcccttatag
      961 ataatctgga tattgataac gaatatgaga aaaagatatt agagttaata atcgaacacc
     1021 gtttcgtaac aagagaggct ttggaagaaa aactaaaaat ccacaaaaat acaattttaa
     1081 aatatttaaa aaaattagtg gaaaaacaa
//
LOCUS       DAVLTM010000218         1107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVLTM010000218 DAVLTM010000000
VERSION     DAVLTM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.219"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            314..>1107
                     /locus_tag="VYN16_002117"
     CDS             314..>1107
                     /locus_tag="VYN16_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HEV1404562.1"
                     /translation="MNKYEERYQEDLSKNDFEKLINRRYLSDKELQVEYVKKGTVLPP
                     KVFEMKLSNKLGLQKALHGKGGVVDSKGNYVELSEQKAVGMRNRVYGSYKFNHKNLAI
                     RNEKVIYLNYFINQWGHFLLDVVGRLWYPLLKDTDTKLDYTCYAGTETKLEGNYLEFL
                     ELLGIDKSRLILINRPTQFSEIIIPESSILPGEYYTKEYKMLFNSLVANVKLDNNLES
                     KKIYCSRARLDLAKGKEFGENGIEKVFLKNGYTPVYMETMSLKEQI"
BASE COUNT          431 a          136 c          192 g          348 t
ORIGIN      
        1 agataaagat ttagatattt tagacatttc aaatactatt cttgatcact atagtgggaa
       61 ctttagagtt tataaagcgg cagtttccta taaagtttcg gcatgtttta gaattttgtt
      121 gaactcatct tcagaaaaaa aatataatca aatacaaaaa gattgtatga catatatttt
      181 acgaaattgg agaaatgtgt tatttgataa aaatgtccga ttaaagaaca agcttgcctt
      241 gatttctata actcttttca atccttttgt taaatttatc tatagcaaag taaacagatg
      301 ggaataatag caaatgaaca aatatgaaga aagatatcag gaagatttat ctaagaatga
      361 ttttgagaaa ctaatcaata gaagatacct atcggataaa gaactacaag ttgaatatgt
      421 aaaaaaagga acagtgttac ctcccaaggt ctttgaaatg aaactatcaa ataaacttgg
      481 tctacaaaaa gctttgcatg ggaaaggtgg tgttgtagat tcaaagggga attatgttga
      541 actttctgaa caaaaagcag ttgggatgag aaatcgagtc tatggatctt acaaatttaa
      601 ccataaaaat ttggctatca gaaatgaaaa agttatttac ctaaactatt tcataaatca
      661 gtggggacat tttttactag atgttgtggg gagattatgg tatccattac taaaagatac
      721 tgatactaaa ctggattaca catgttatgc tggtacagaa acgaaacttg aaggaaacta
      781 tttagagttt ttagaactcc tcggtataga taaatcacgc ttaattttga taaatcgccc
      841 aactcaattc tcagagataa ttatacctga aagttcaatt ctgccaggtg aatattatac
      901 aaaagaatat aaaatgcttt ttaattcttt agtagcaaat gttaaattag ataacaattt
      961 agaatcaaaa aaaatttatt gttcacgagc taggctagat ttagcaaaag gtaaagagtt
     1021 cggtgagaat ggtattgaaa aggtcttctt aaaaaacggt tatactccag tttatatgga
     1081 aacaatgtct ttgaaagaac aaataag
//
LOCUS       DAVLTM010000219         1092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVLTM010000219 DAVLTM010000000
VERSION     DAVLTM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.220"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            74..652
                     /locus_tag="VYN16_002118"
                     /pseudo
     CDS             74..652
                     /locus_tag="VYN16_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            665..835
                     /locus_tag="VYN16_002119"
     CDS             665..835
                     /locus_tag="VYN16_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404563.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          397 a          134 c          210 g          351 t
ORIGIN      
        1 tttggattac gaatgaattt gagtgctggt attatcaatc atgaactagg attctagttt
       61 tgcaggaagt ctattatcaa aaataccatc aggattatat aagattgata caggaaaagt
      121 tttatttgat ggtgtaaata ttaatcaaat agataaaaaa atattaagtc aaaatttagg
      181 agtagttcca caggattcgt ttttattgaa cagaagtatt cttgataaca taactttaaa
      241 gcacgaagtt acttcacaaa agatagagga agtttgtaaa gcagttcaaa tctatgatga
      301 aatcatggct atgccgatga aatttaatac tatcatctca gagatgggtt caaatatttc
      361 aggtggtcaa agacaacgga tagcactggc acgtgcatta ataaataatc ctagtattgt
      421 aattttagat gaagcaacta gtgctttaga cactattaat gaggaaagaa taacaaagta
      481 tataaaaagt cagggctgta ctcaaataat tgtagctcat agattgtcaa cgattaagga
      541 tgcggatatt attgttgtaa tgaaaggtgg taagattgtt gagtcaggaa atcataagta
      601 cttaatggat cttggtggag agtactacag cttatataca aaaaggaaat gaggtgtaaa
      661 gaaaatgaag aaagaaaatg aatatgtaat tttaacaaca gcctcactag gggtgatgat
      721 tggaatagtg tttgcaattt ttttagattt tccagttgaa tatggtattt ctttaggctt
      781 attgaatgga atagtattgg gttcgctgat tgtttacaaa aacaataaga attaagcata
      841 attttttgct gtaaactaag gagtagagat ggctatagtt gaaattataa atctaacaaa
      901 aagctttaaa gatattgaag ttattcataa cacttaaata atagagcaac tacagtagta
      961 gcttaaaaac atgattaaat cgctattctt aggagtagcg gtttttcttt ttgtttaata
     1021 ctctttgaaa atctcttcaa accacgtcag ctttgccttg ccgtactcaa gtacagcctg
     1081 cgactcgctt cc
//
LOCUS       DAVLTM010000220         1066 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVLTM010000220 DAVLTM010000000
VERSION     DAVLTM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.221"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..659
                     /locus_tag="VYN16_002120"
     CDS             <1..659
                     /locus_tag="VYN16_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1404564.1"
                     /translation="KQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVT
                     VTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDT
                     FIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDST
                     KDVTATVLDKNNISSKSTTNNLNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD
                     "
BASE COUNT          422 a          134 c          200 g          310 t
ORIGIN      
        1 ttaaacaaat aacagacaag acactaaaca ttaagattgt tgtaaaagat tttgcaagaa
       61 atacaaccgt aaaagaattc attttaaata aagatacggg agaggtcagt gaattaaaac
      121 ctcatagggt aactgtgacc attcaaaatg gaaaagaaat gagttcaacg atagtgtcgg
      181 aagaagattt tattttacct gtttataagg gtgaattaga aaaaggatac caatttgatg
      241 gttgggaaat ttctggtttc gaaggtaaaa aagacgctgg ctatgttatt aatctatcaa
      301 aagatacctt tataaaacct gtattcaaga aaatagagga gaaaaaggag gaagaaaata
      361 aacctacttt tgatgtatcg aaaaagaaag ataacccaca agtaaaccat agtcaattaa
      421 atgaaagtca cagaaaagag gatttacaaa gagaagatca ttcacaaaaa tctgattcaa
      481 ctaaggatgt tacagctaca gttcttgata aaaacaatat cagtagtaaa tcaactacta
      541 acaatcttaa taagttgcca aaaactggaa cagcaagcgg agcccagaca ctattagctg
      601 ccggaataat gtttatagta ggaatttttc ttggattgaa gaaaaaaaat caagattaag
      661 ataaaagcta tagaaaaaaa tggtttatgt actgagatta gatagtgagg tgatgacata
      721 gttttgtgaa aacagccatt tataactcaa ttatttagtt tacttcacta ttgatactat
      781 ttggagttat taatggactt agtttagata actaatgaat tgattgaaag ggttagtatt
      841 gacaatattg gtcatattga ttagaaaata gagtctatca aaatttaaag gctaatagag
      901 gtgatgagac aatttcaagg aattaagtag cgtttggttg cttaattcct ttttatttgg
      961 aaggaagttt ttcgtaaccg cccaataacg aagtctattg gacggttacg atatgatcac
     1021 tgacttcgtc agtcttatct acaacctcaa aactgtgttt tgagca
//
LOCUS       DAVLTM010000221         1058 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVLTM010000221 DAVLTM010000000
VERSION     DAVLTM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.222"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            316..501
                     /locus_tag="VYN16_002121"
                     /pseudo
     CDS             316..501
                     /locus_tag="VYN16_002121"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          411 a          190 c          146 g          311 t
ORIGIN      
        1 gccacatagt actgaactct ctccaaaaac ggttatgcct ctttgaatag ggcgttatca
       61 caagaaaagc atctccacgt ttcaacttca tatggctcaa aaacaatcaa ttgatgctaa
      121 aacctgtacc tagatgtttc ggttcataaa accatgaaac tgtaaaagtg gatgaaattg
      181 atagcgatag tcaaatcaag aggcatcata actctaaaaa gtcacaatat ataagttcat
      241 cctcggaaaa atatcattct aattgttgaa atgcctacat gaaaagaaac gtcaaatact
      301 catgaaacaa cgaatacagg tatcaaaact atgacaaaac aagtccctaa atttactaaa
      361 gacactgctc aactttacac ctgtaaatgg ttgttgtata ataaagttac aaagatgtac
      421 gaccacactg ttgtaaatca tagtgttcgc gaatatatta ctgatagcat ttctacaaat
      481 acaattgaaa aggctggata ggctttaaac gaaatatgtc agtgttttgg cattcctagc
      541 cttcatatca tttaaagaat tgtatagaca aaatttttcc caatacagac actcgtaaca
      601 actgcttcat ttttctacca acatatttag gaacaggata agatacaaga ggattaatcc
      661 atagatcagt tctataccaa tctaagacaa ataagctaaa aaaacgattg ataataagca
      721 aatagattcc aaattttctc tatctgctca ttttaataaa caatactagt gtaactatcc
      781 ttccagtcag aagcttgtca aatcacaccg aaaattcttc taaaatttat ctcgttaggc
      841 aatcaagcaa aaactcgacg atagtacaaa cattatcata cagaattgac ttcctaaatt
      901 atatacttta gtaaggtttt cggataagaa aaaaggttca ttttacattt ctaaacattc
      961 ttttctaaga tgaaaaacag aatttttcga ttgtgattta aagcaacaag aagattttca
     1021 gtatcatcct atagatacga gctaattaag aaaaacta
//
LOCUS       DAVLTM010000222         1037 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVLTM010000222 DAVLTM010000000
VERSION     DAVLTM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.223"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            101..283
                     /locus_tag="VYN16_002122"
     CDS             101..283
                     /locus_tag="VYN16_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1404565.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            293..658
                     /locus_tag="VYN16_002123"
     CDS             293..658
                     /locus_tag="VYN16_002123"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1404566.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            655..1037
                     /locus_tag="VYN16_002124"
                     /pseudo
     CDS             655..1037
                     /locus_tag="VYN16_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          431 a          128 c          173 g          305 t
ORIGIN      
        1 aagaggatag aaatgaccga attagaaaga aaaaatcgaa aaattagcta agaaatattc
       61 tgataactta aacatcaaag ttcaagagag agttcgtgaa atggcaaatg ataataagag
      121 ccattatttg atatacagag ttttaggtat ttcatttgaa gaaggagaaa atatcgattt
      181 gtatcaaaat aaaggtcgtt ttttatacaa atatgctggt tcatttttag aagaagctgc
      241 agtactatcc tttaacgaaa aatttggtac agaaaatact taaaaagtta acattcctaa
      301 ttctgaaagt acaaaaccta agacttttga aattgattgt ttagtcggag aaaaacacgc
      361 atacgaaata aaatggtggg atgcaactac agatggagac catataacta aagaacacac
      421 tagaataaaa gttattcata acaaaggata tataccaatt cggttaatgt tctactatcc
      481 aaatagaact caagctataa aaattcagca aactttagaa acattgtata acggtattgg
      541 agggaaatat tattatggag attctgcctg ggaacattta agagcagtga ccggtattga
      601 tttacttagt attctaacag atattgcaaa taaaaaaaca ggggtaaaat caaaatgaca
      661 gtattaaaag gagataactt agaaatatta aaaactattg aatcctcaag tattgattta
      721 atctatatgg accctccttt ctttacacag aaaacccaaa aattatctaa taacaaaaat
      781 attatgtatt cattcgaaga tacgtggact tcgattgagg attacaaaga atttttgtct
      841 gtaagattag aagaatgcaa aagagtgcta aaaaatagtg gcagtatttc gttcattgtg
      901 ataaaattgc aaatcatcat attagattaa ttttagataa tatctttgga gcagatatgt
      961 ttcaaagcga aattatatgg aactataaac gatggtctaa ttcaaaaaag ggattattga
     1021 acaatcatca aaacatt
//
LOCUS       DAVLTM010000223         1025 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVLTM010000223 DAVLTM010000000
VERSION     DAVLTM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.224"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..427
                     /locus_tag="VYN16_002125"
     CDS             <1..427
                     /locus_tag="VYN16_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diadenosine tetraphosphate hydrolase"
                     /protein_id="HEV1404567.1"
                     /translation="VYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSDMPMEFRKEFL
                     MEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRKTVVHRYDSSV
                     FSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
BASE COUNT          355 a          118 c          183 g          369 t
ORIGIN      
        1 tgtttatgaa tttgatacta gctttgtaga aatgtcttat tctcagtttt ttgaaggata
       61 ttgtattctt ttttcaaaag aaccctatcc ttctttatct gatatgccaa tggaatttag
      121 aaaagaattt ttaatggaaa tgagtatatt aggtgatgcg atgcttgaag tgttaaatgc
      181 ctatagaatt aattatgata tcttaggaaa ttcaacacct gtacttcatg ctcatttatt
      241 tccgagatac atgtgggaag aggaagagag aagaaaaact gtagttcata gatatgattc
      301 ttcagttttt agcaatcctg agtttgaatt tactatagaa aagcatggac caattattaa
      361 aaaactgagg tctgctattg atagacatta tagagagtat caacaattat tagatgattc
      421 aaactgaact ccacattttc gattagattt aataccttgt atattacaac taggttgtgg
      481 atgatataat tttttgaaaa atatatttta ggaatgactt ataaagcaat tatatcagag
      541 gctattaaat caatggccaa agcttatgct ccttattctt attttaaggt tggagctgcg
      601 ttagaaactg aagataaaaa ttttttcctg gatgtaatat agaaaatgtt gcttttctcc
      661 tacttgctgt gcagaacgag tatattttta caaagctgta tttaaaggag aacgttcgtt
      721 ttcaaaaata tgtattgtag gcggaaaaaa aggtatttta tcatctttta tagcatcttg
      781 cagggtgtct tgtcaggtaa tgatagaatt ttgtaatctt gattcatttg tagttatatt
      841 ggctagtgat gttgataatt ataaagtata taaacttaaa gaactactac cagttagttt
      901 taatcaagaa aatttataac aggagggaac tatggaatta gttgaattta aaaatggaca
      961 tgttaatacg gttcatgagc atatcgcaat aacgaaagta aaagagtttc taaataatca
     1021 tgata
//
LOCUS       DAVLTM010000224          998 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVLTM010000224 DAVLTM010000000
VERSION     DAVLTM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.225"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            61..411
                     /locus_tag="VYN16_002126"
     CDS             61..411
                     /locus_tag="VYN16_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1404568.1"
                     /translation="MLRHPCISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            626..742
                     /locus_tag="VYN16_002127"
     CDS             626..742
                     /locus_tag="VYN16_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1404569.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            735..968
                     /locus_tag="VYN16_002128"
     CDS             735..968
                     /locus_tag="VYN16_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404570.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGREERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT          311 a          195 c          223 g          269 t
ORIGIN      
        1 cttttctgat agagttgttc acatcttatt tcaactcact atagaaggag gaaaaggagg
       61 attctcagac atccgtgcat cagcccaact aatgatttga ttgctaagaa aatattcagc
      121 aatccagaaa tcacttgtca atttattcgc gatatgctgg acttgccagc aaaaaatgtg
      181 accattttgg agggaagcga tattcacgta ttactctcca tgccttactc ggtgcaggat
      241 ttttatacca gtatagacgt cttggcggag ttggataacg gtactcaagt aattattgag
      301 attcaagtcc atcatcagaa ttttttcatc aatcacttgt ggacttacct gtgcagtcag
      361 gttaatcaaa atcttgaaaa aattcgtcag cgagaaggtg atactcacta gagctacaaa
      421 cacatcgctc ctgtttacgc cattgctatc gtggatagta attatttctc agatgacctg
      481 gcttttcata gctttagtat gcgcgaagac acaacaggtg aggtattggc gattaccaac
      541 aatggacagg aaaaccatct ggttaagatg gcattcttgg aattaaaaaa tacagagaaa
      601 ccagcaaaga caaggttcgc aagccatggt tggagttttt cggcaacaag ccctttaccc
      661 agcaaccgca acgagccatt acccaagcaa atcaactgct ggactacaag agctggtccg
      721 aggaggacag gaaaatgttt agtcaactac atatgcgaga agaacaagtc ttgttagcac
      781 aggactatgc cttggaaact gctagggctg aaggccttga acaaggacta gagcgtggga
      841 aagttgaagg aagggaagaa aggaaacttt ttgccttcct agacatagta cgccaaggtc
      901 ttctgacttc tgaggttgcc agccagcaat taggtatgtc agtatctgaa tttgaggcac
      961 tgttgtaaaa tggctctata atatttgtag tgtgtaaa
//
LOCUS       DAVLTM010000225          957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVLTM010000225 DAVLTM010000000
VERSION     DAVLTM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.226"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..757)
                     /gene="lytA"
                     /locus_tag="VYN16_002129"
     CDS             complement(<1..757)
                     /gene="lytA"
                     /locus_tag="VYN16_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1404571.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFN"
BASE COUNT          248 a          238 c          178 g          293 t
ORIGIN      
        1 cgttgaaata gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt
       61 tgtcgaacca gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc
      121 ctgaactgtc aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag
      181 agccgtctga atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg
      241 tcaagccgtt ctcaatatca tgcttaaact gctcacggct aatgccccat tttgccaagt
      301 aagggtatgg atccacatgg tctgagtggt tgtttggttg gttattcgtg caatactcgt
      361 gcgttttaat tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg
      421 ctagattgcg taagagttcg atataaaggc ggtagtccgt catgaactct tctttagttg
      481 aatggctttc aatcagttca accgctgcat aggtctcagc attccaaccg cccccaacgt
      541 cccaggcacc attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg
      601 agaaaaaacc taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg
      661 aatgcggatt cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag
      721 gcaaatctgt tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa
      781 caactcattt tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac
      841 aatcttagtc acgattaggc ttgtagatag aacttcaaaa cgcactattt tgacactgta
      901 aataggactg acaaggtctg cattctatct acaataacac cccagactaa aaagctt
//
LOCUS       DAVLTM010000226          939 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVLTM010000226 DAVLTM010000000
VERSION     DAVLTM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.227"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..911)
                     /locus_tag="VYN16_002130"
     CDS             complement(<1..911)
                     /locus_tag="VYN16_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404572.1"
                     /translation="MVAGQLLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGI
                     IFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLN
                     DQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSL
                     NLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAG
                     AISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIG
                     TKPIFLKLYSINPIESNKMNDIEPNEV"
BASE COUNT          333 a          167 c          126 g          313 t
ORIGIN      
        1 ttcacctcat ttggttcgat atcattcatt ttatttgact caattggatt aattgaatac
       61 aattttaaaa aaataggctt cgtaccaata atagaggata attgacctcc taattcacct
      121 agcgctgtaa aaataacacc tgttagtgct cctattgctt caatagtacc aattttcact
      181 attcctttta ttgcaagata gcctgttaaa aaaacgagag atatctgaaa aaaaatattg
      241 agaaagaagc taatagcgcc tgctaacgtt tctacagttg tctttctttg tataaccatc
      301 tttaataaaa ttcctgcttc tttaattttc ttaggcaata catataaaag attcaaggac
      361 gctaacacat caaatccatt caatatagtc tcactagatt ttaaaaaagc ttcattttgg
      421 ctagttaaat ttagactaac ttctcgcatt ttcgatgcaa agatttttgg tacaagtagc
      481 ataatcatta atgaaaacaa ggtggctaca gtcaatgacc aatgatagtg attaagagtc
      541 acaactgcaa atatagtacc agaaattcct tttattacta aaaaaagttg tttaaaagcc
      601 tgatcattta aagtctgaac atcattattt agccacgaaa gatatgttcc tgatgattta
      661 ctatgaaatt cttgataggt agagttagag atgtctgtgg caactctatt tcgaatctct
      721 agattaaact cttggatcac ttcaacctga taatttttca ctacccagtc aaggaatatt
      781 atcccacacc agacaatcat ttggtagatt gacaatttca aaaaccgctc taaattcatc
      841 gcaattaatt cattcaacac cagagcatta atagttgctg cataaattag caataattga
      901 ccagcaacaa taaatatcgt taataaacta aattttttt
//
LOCUS       DAVLTM010000227          938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVLTM010000227 DAVLTM010000000
VERSION     DAVLTM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.228"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(1..69)
                     /locus_tag="VYN16_002131"
     tRNA            complement(1..69)
                     /locus_tag="VYN16_002131"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Leu,seq:caa)
     gene            complement(87..158)
                     /locus_tag="VYN16_002132"
     tRNA            complement(87..158)
                     /locus_tag="VYN16_002132"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Gln,seq:ttg)
     gene            complement(169..241)
                     /locus_tag="VYN16_002133"
     tRNA            complement(169..241)
                     /locus_tag="VYN16_002133"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:His,seq:gtg)
     gene            complement(256..326)
                     /locus_tag="VYN16_002134"
     tRNA            complement(256..326)
                     /locus_tag="VYN16_002134"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Trp,seq:cca)
     gene            complement(331..411)
                     /locus_tag="VYN16_002135"
     tRNA            complement(331..411)
                     /locus_tag="VYN16_002135"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Tyr,seq:gta)
     gene            complement(424..496)
                     /locus_tag="VYN16_002136"
     tRNA            complement(424..496)
                     /locus_tag="VYN16_002136"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(461..463),aa:Phe,seq:gaa)
     gene            complement(500..573)
                     /locus_tag="VYN16_002137"
     tRNA            complement(500..573)
                     /locus_tag="VYN16_002137"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(537..539),aa:Met,seq:cat)
     gene            complement(584..673)
                     /locus_tag="VYN16_002138"
     tRNA            complement(584..673)
                     /locus_tag="VYN16_002138"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Ser,seq:tga)
     gene            complement(756..829)
                     /locus_tag="VYN16_002139"
     tRNA            complement(756..829)
                     /locus_tag="VYN16_002139"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(793..795),aa:Ile,seq:gat)
     gene            complement(864..934)
                     /locus_tag="VYN16_002140"
     tRNA            complement(864..934)
                     /locus_tag="VYN16_002140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(900..902),aa:Gly,seq:tcc)
BASE COUNT          257 a          261 c          221 g          199 t
ORIGIN      
        1 ggccggggtc gaaccggcac gtccttgcgg acactggatt ttgagtccag cgcgtctgcc
       61 aattccgcca cgccggcaaa tagtaactgg ggtagctgga ttcgaaccaa cgcatgaggg
      121 agtcaaagtc ccttgcctta ccgcttggct ataccccaat ataaaatagg cgagtgatgg
      181 ggatcgaacc cacgcatgcc agagccacaa tctggtgtgt taaccacttc accacacccg
      241 ccataattct attaacacgg gcagtaggaa ttgaacccac actgaaggtt ttggagacct
      301 tagttctacc tttaaactat gcccgtaaaa tggaagggga gggattcgaa cccccgaacc
      361 cgaaggagcg gatttacagt ccgccgcgtt tagcctcttc gctacccttc caaaatatat
      421 aaatggcgcg agacggaatc gaaccgccga cacatggagc ttcaatccat tgctctacca
      481 actgagctac cgagccttat tgcgggagca ggatttgaac ctacgacctt cgggttatga
      541 gcccgacgag ctaccgagct gctccatccc gcgttaatat aaaaggagga tgtgggattc
      601 gaacccacgc acgcttttac acgcctgacg gttttcaaga ccgttccctt cagccggact
      661 tgggtaatcc tccaaaatag tttatacggg aacaagcccg tgtcctctta gtgaacagat
      721 aatattccaa tcccctagta atggactaaa cactatgggc acgagtggac tcgaaccacc
      781 gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc tacgcgccca agtcaaaaac
      841 ttggtacaaa gaacaaagtt caaagcgggt gacgagaatc gaactcgcga caacagcttg
      901 gaaggctgta gttttaccac taaactacac ccgctaaa
//
LOCUS       DAVLTM010000228          923 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVLTM010000228 DAVLTM010000000
VERSION     DAVLTM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.229"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            34..873
                     /locus_tag="VYN16_002141"
     CDS             34..873
                     /locus_tag="VYN16_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1404573.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
BASE COUNT          298 a          146 c          200 g          279 t
ORIGIN      
        1 caatctgaat cctaaaatag aaagattgat actatgacaa aaattaagat tgtaaccgat
       61 tcatctgtta ctattgaacc tgaactagta aagcaattag atattacaat tgttccatta
      121 tctgtaatga ttgataatgt tgtttattct gatgcggatt tgaaagaaga aggtaaattt
      181 cttcagttga tgcaagaaag taagaatctt ccgaaaacaa gtcagccacc tgtaggtgtc
      241 tttgctgaga tttttgaaga cctatgcaaa gatggtggcc agattcttgc tattcatatg
      301 tcccatgctc tttcgggtac ggtagaagca gcacgccaag gtgctagcct atctactgca
      361 gatgtgactg ttgttgatag ttccttcact gaccaagccc tgaaattcca agttgttgag
      421 gctgcgaagt tagctcaaga aggtaaagat atggaggcaa ttttatgtca tgtagaagag
      481 gttaaaaacc atacagagct ttatattggt gtttcaactt tggaaaatct tgtcaaaggt
      541 ggacgaatta gccgtgtaac tggcttgttg agctctcttc tcaatatccg tgttgtcatg
      601 caaatgaaag accatgaatt gcagccaatg gttaaaggtc gtggaactaa aacatttaaa
      661 aaatggttag atgagttgat aacatcgctt tctgaacgtg ctgtagcaga gattggaatt
      721 tcatattctg gtagtgatga ttgggcaaaa gagatgaaag aaagcttaca agcttatgtt
      781 gaaaagccaa tttctgtttt ggaaacgggt tccattattc aaactcacac gggtgagaac
      841 gcttgggcta ttttgatacg ttaccactcc taaaaaaata aataaaatgg gaagaaatag
      901 cgtttttaac ttgacctaaa agg
//
LOCUS       DAVLTM010000229          902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVLTM010000229 DAVLTM010000000
VERSION     DAVLTM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.230"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..902
                     /locus_tag="VYN16_002142"
                     /pseudo
     CDS             1..902
                     /locus_tag="VYN16_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          276 a          191 c          166 g          269 t
ORIGIN      
        1 acaaggaaat catcgccaaa ctggactacg acgccccatc ttgccctgag tgcggaagtc
       61 aaatgaagaa atatgacttt caaaaacctt ctaagattcc ttatcttgaa acgactggta
      121 tgcctactag aattctcctt agaaagcgtc gattcaagtg ctatcactgt tcaaaaatga
      181 tggtcgctga aacttctatc gtcaagaaga atcaccaaat ccctcgtatc atcaaccaaa
      241 agattgcgca aaagttaatt gaaaagattt ctatgactga tattgcccat cagctttcca
      301 tctcaacttc aactgttatt cgtaagctca atgattttca ctttgagtgt aattttagaa
      361 atctgcctaa gattatgtct tgggacgttg aaacagtcca gggagtgact atttcaatcg
      421 ggagatggag atgagcttta ttgcgcaaga ttttgaaaag ctcgatatca tcactgttct
      481 taagggaaga acacaagctg tcatccgaaa tcactttctt aaatatgata gagccgtctg
      541 atgtcgcgtc aaaatcatta ctatggatat gtttagccct tactatggct tggctaaaca
      601 gcttcgcttt caaatttcta ggctcaggct gaaacagtct cccagactgt ttcactcccg
      661 aatgctaaaa tcgttcttga acgctttcac attgtgcaac atcttagccg tgctatgagt
      721 cgtgtgcgtg ttcaaatcat gaatcagttt catcgaaaat cccatgaata caaggctatc
      781 aagcgctact ggaagctcat acaacaggat agccgtaaac tgagtgataa gcgattttat
      841 cgtcctactt ttcgtatgca tttaccaata aagagatttt aaacaagctt ttgagttatt
      901 ca
//
LOCUS       DAVLTM010000230          889 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVLTM010000230 DAVLTM010000000
VERSION     DAVLTM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.231"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..193
                     /locus_tag="VYN16_002143"
     CDS             <1..193
                     /locus_tag="VYN16_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1404574.1"
                     /translation="ELNSLLNAIVEENNTTKTDFIRQAVIEKIEDMYDIKVADEAYKK
                     WVECGKKAISHEEMMRRYG"
     gene            186..>889
                     /locus_tag="VYN16_002144"
     CDS             186..>889
                     /locus_tag="VYN16_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1404575.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GKLWKELTSSEIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYFI
                     DGDVTNSKTIPILCYDT"
BASE COUNT          334 a          120 c          153 g          282 t
ORIGIN      
        1 agagttaaat tctttattaa atgctattgt tgaagaaaat aatacaacaa agactgactt
       61 tattagacaa gctgttatag agaaaattga agatatgtat gatattaagg tcgctgatga
      121 agcttataaa aaatgggttg aatgtggcaa aaaagctatc tcacatgaag aaatgatgag
      181 acgctatggc taaattatac gcaatatctg atatacatgg atacttggat gaattcagag
      241 acgctctcaa taaagtaaat ttaaacgata aagataatcg cctattttta ctaggagatt
      301 atcttgataa tggacttcaa tcttttcaag tcatttccaa aataatcgaa cttgaggaag
      361 tttatcctaa tcaaattatt actcttctag ggaatcatga agaatggttt tatgattggt
      421 tgattttaga taagcctact gcttcagcat ttccagaaac aataaaaagc ttcttctctc
      481 cagaggaatt gaactatatt tttaaatcga atgctaacaa ctttgaaaca ggtgttagaa
      541 atgaaattaa aaataatatc aaattcaatc catttataaa ctggtttaaa aaacgatata
      601 gggataaacg ctattatgaa actgataccc aaatttttgt tcatgctggc attgatgaag
      661 aagccggaaa gctttggaaa gaactaacga gttcagagat atttacaaac aaatttccta
      721 ttaccactgg tagatttcat aaagctatta tttcgggcca tattgcttct tgggaagtag
      781 ctaaagatag aagatacctg ggtaagattt actatgatag taaaagtcat tattttattg
      841 atggagatgt aacgaatagc aaaacaatac ctattctttg ctatgatac
//
LOCUS       DAVLTM010000231          885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVLTM010000231 DAVLTM010000000
VERSION     DAVLTM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.232"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..885
                     /locus_tag="VYN16_002145"
                     /pseudo
     CDS             1..885
                     /locus_tag="VYN16_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          263 a          187 c          163 g          272 t
ORIGIN      
        1 gctccatctt gtcctgagtg cggaagtcaa atgaagaaat atgactttca aaaaccttct
       61 aaaattcctt atcttgaaac gactggtatg cctactagaa ttctccttag aaagcgtcgt
      121 ttcaagtgct atcattgttc taaaatgatg gtcgctgaaa cttctatcgt caagaagaat
      181 catcaaattc ctcgtattat caaccaaaaa attgcgcaaa agttgattga gaagatttct
      241 atgaccgata ttgctcatca gctggccatt tcaacttcaa ctgtcattcg aaagctcaat
      301 gattctcact ttgagcatga tttttcgcgt cttcctgaga ttatgtcctg ggacgttgaa
      361 acagtccggg gagtgactgt ttcaatcggg agatggagat gagctttatt gcgcaagatt
      421 ttaacaatct caatatcatc actgttcttg aaggcagaac acaagctgtc atccgagatc
      481 actttcttaa atatgataga gccgtccgat gtcgcgtcaa aatcattact atggatatgt
      541 ttagtcctta ctatgacttg gctaaacagc ttcgctttca aatttctagg ctcaggctga
      601 aacagtctcc cagactgttt cactcccgaa tgctaaaatc gttcttgatc gctttcacat
      661 tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtt caaatcatga atcagtttca
      721 tcaaaaatcc catgaataca aggctatcaa gcgctactgg aagctcatac aacaggatag
      781 ccgtaaactg agtgataagc gattttatcg tcctactttt cgtatgcatt taaccaataa
      841 agagatttta gacaagcttt tgagctattc acaagacttg aaaca
//
LOCUS       DAVLTM010000232          870 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVLTM010000232 DAVLTM010000000
VERSION     DAVLTM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.233"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            250..>870
                     /locus_tag="VYN16_002146"
     CDS             250..>870
                     /locus_tag="VYN16_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404576.1"
                     /translation="MRGETLNSYITTFTQAITNDPNYKEICKKIGVNLNEYLNKQYPI
                     LHHNKNYQNFKMIQKNLKEFEAFAKLTHTIGNFTVLPHWMNTGRYNFSKDYWDITMLS
                     LQEWLTNLSPQAWKNFIDTYYLQPYVDNNYQTEVFWETHNYEYIYPKKTEDFSIFLKR
                     VNERIEERGKFIIKQICDKLNKKDFHFYKEIENMDKIKYSNEFEQER"
BASE COUNT          349 a          139 c          128 g          254 t
ORIGIN      
        1 aaagttattg aatggttaaa ttactgtgac caaaacgata tcaaaccatg gttatgggat
       61 tttaaaaatt gttactctag cgagttaacc gcagataata tttcaaattt tctaaaatat
      121 aaaaatggag aactcgtaaa tccttccaca ttttgtatca caaaacaggt tggtagtaac
      181 gctgactgcg ataaagaggc actcccactt tataatgttt taggttggca acattccgag
      241 agagatataa taagaggtga gaccttaaat tcttatataa ctacatttac tcaagctatt
      301 accaatgacc cgaattataa agaaatttgt aaaaagattg gagtaaactt aaatgaatat
      361 ctaaacaagc agtatccaat cttacaccat aataaaaact atcaaaattt caagatgatt
      421 caaaaaaacc taaaagaatt tgaagcattc gcaaaattaa cacatacaat cggaaacttt
      481 actgtattgc ctcattggat gaatactgga cgatacaatt tctcaaaaga ttactgggat
      541 attacaatgc tttctcttca agaatggctt actaatttat ctcctcaagc atggaaaaat
      601 tttattgata catactatct tcaaccctat gttgataata actatcaaac agaggtattt
      661 tgggaaactc ataactacga atacatttat cccaagaaaa ccgaagattt ctccatcttc
      721 ctaaaaagag tcaatgaacg gattgaggag cgcgggaaat tcatcattaa acaaatatgc
      781 gataaactaa ataaaaaaga tttccatttc tataaggaaa ttgaaaatat ggataaaatt
      841 aaatactcaa atgaatttga acaagagagg
//
LOCUS       DAVLTM010000233          868 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVLTM010000233 DAVLTM010000000
VERSION     DAVLTM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.234"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..249)
                     /locus_tag="VYN16_002147"
     CDS             complement(<1..249)
                     /locus_tag="VYN16_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV1404577.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFS"
     gene            427..588
                     /locus_tag="VYN16_002148"
                     /pseudo
     CDS             427..588
                     /locus_tag="VYN16_002148"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            574..>868
                     /locus_tag="VYN16_002149"
     CDS             574..>868
                     /locus_tag="VYN16_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1404578.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILIIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLK"
BASE COUNT          340 a           97 c          145 g          286 t
ORIGIN      
        1 actgaataat aagattaaaa ttggtaaatt agatattaat gctgctccta aatttttcca
       61 ataatcttta aaaaaactgt ttgcgaagtc tctataggga taggattcct cgtgtcgtac
      121 aatttttatt agagttgcta ttagggcact ttcacctgat ccgatagcaa taactgttac
      181 tactgacgct acatagagta gatttaacat tataagattt gaaactttat caataaattt
      241 tatctgcatc tttaactcct gctgttttta tacttataat atcacatcat ttgagtttca
      301 tcaactttga atacattaag aaagaatata actatgagga aaggaaaaaa ttattatgaa
      361 taaatccaaa aaatataaag aaagaattca ttctgaatac gataatctta ctaaatcaga
      421 gaaaaaattg ctgactatat tttagttaga gatagtgaaa tagtttatga aacgtcttca
      481 gaaattgtta gtaaagtaaa agttggagag ggaactctta atcgattttg caaaaagatt
      541 ggatactctg gttttcagga actaaaatta aaaatgacga aagatatatt agagttagaa
      601 tctcaaaaaa tgagtagtga cacatttatt gatgaaataa aaaataatta tttatctatt
      661 gtggaaagta caagaaaatt aattgatggt agacaaatag aattagctat aaaattaata
      721 agagaagcaa accagatttt aattattggt gtgggttcta gtggaaatgc ggctagagaa
      781 tttgagagta gtttgttaag aattggaata ataagtaaga cggtaattga tacccatttt
      841 caactcatgc atactgcgtt attgaaag
//
LOCUS       DAVLTM010000234          864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVLTM010000234 DAVLTM010000000
VERSION     DAVLTM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.235"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(255..593)
                     /locus_tag="VYN16_002150"
     CDS             complement(255..593)
                     /locus_tag="VYN16_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404579.1"
                     /translation="MSYDLEILAKIESGDYICIAEPRYSSPTYNFGKMFRVAMDWDFD
                     QGTIYNVADIFENIKRGITELERQPEKYVQYEPANKWGTINDALYVLRSLRDCILEQD
                     IDTKYLYVRW"
     gene            complement(593..745)
                     /locus_tag="VYN16_002151"
     CDS             complement(593..745)
                     /locus_tag="VYN16_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404580.1"
                     /translation="MSYTVTLYFDNMVDETHFFKKEGDAAKCKAQLESKYRGDRMYKV
                     KMEEME"
BASE COUNT          234 a          189 c          108 g          333 t
ORIGIN      
        1 cggtctttca ccgcttgtgg taaattttgc ttgggaggtt gcggcttccc gtcttctacc
       61 gtccagttgt atactgcatt aacttttttc tttaactctt ccatcacttc aactccttgt
      121 ccttaatttc tccagtaagt ctattttcta aaatgtgact tgtatagcaa atatcgtttt
      181 tataagtata gtgatcaacg gtttcttcaa cccattgatt tttagtgtac gggtatctgt
      241 ttggtcgttt caatttacca cctcacatat aaatatttcg tatcaatatc ttgttctaaa
      301 atacagtccc ttaacgatct caaaacatat aacgcatcat tgatcgttcc ccatttattt
      361 gcaggttcat attgtacata tttttcaggt tgcctttcca attcagttat gccacgttta
      421 atattttcaa aaatatcagc aacattgtaa attgtgcctt ggtcgaaatc ccaatccata
      481 gccaccctaa acatttttcc aaaattgtag gtcggagaac tatatctagg ttcagcaata
      541 caaatataat ctccactctc tattttcgct aagatttcca aatcataact cattactcca
      601 tctcctccat ctttacttta tacattcgat cacctcgata cttgctctcg agctgagcct
      661 tgcattttgc agcatcaccc tctttcttaa aaaagtgggt ttcgtctacc atattgtcaa
      721 aatatagtgt tactgtgtat gacattttta cctctttttt tcaaactgct accgtgctac
      781 cgataaaatc taaaaaaata aaaagttttt tcaagaatcc ctattttata ggctttcttt
      841 attattacta ttattttata tact
//
LOCUS       DAVLTM010000235          840 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVLTM010000235 DAVLTM010000000
VERSION     DAVLTM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.236"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..725
                     /locus_tag="VYN16_002152"
                     /pseudo
     CDS             1..725
                     /locus_tag="VYN16_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          281 a          178 c          126 g          255 t
ORIGIN      
        1 gtcgctgaaa cttctatcgt caagaagaat catcaaattc cttgtatcat caaccaaaag
       61 attgctcaga agctaattga aaagacttct atgaccgaca ttgattatca gttgtctatt
      121 tcaactgtca ttcgcaagat caatgatttt cactttgagc atgatttttc gcgtcttcct
      181 gagattatgt cctaggacgt tgaaacagtc cagggagtga ctatttcaat cgggagatgg
      241 agatgagctt tattgctcag gactttgata agctcaatat cataactgtt cttgagagca
      301 gaacacaagc catcatacga aatcccatga atacaaggct atcaagcgct actggagctc
      361 attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac ttttcgtatg
      421 cacttgacta ataaagaaat tctagataaa cttttgagct attcgaagac ttgaaacacc
      481 actataatct ctatcagcta taatctctat cagctcttgc ttttccacct ctttttcaga
      541 ctctgtttaa aaccttcctc aaagataaag aaaaaatcgt caacggcctt caattacctt
      601 attccaacgc caaactggaa gccaccaata atctcatcaa acttatcaag cacaatgcct
      661 ttggttttag gaactttgaa aacttcaaaa aagaaaggac gaaatttgtc ctttctcaag
      721 cttagctttt cttcaaccca ctacagttga caaagagcca ctatttttgg ttcattttac
      781 tataacacga aatagattat tattacataa taaaaaagag gtctaaacct cttaactaaa
//
LOCUS       DAVLTM010000236          833 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVLTM010000236 DAVLTM010000000
VERSION     DAVLTM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.237"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..833
                     /locus_tag="VYN16_002153"
                     /pseudo
     CDS             1..833
                     /locus_tag="VYN16_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          325 a          133 c          169 g          206 t
ORIGIN      
        1 ttctaatatt aggtacatga ttaaattgat tgatcgttac ggaatagagt tcgtcaaaaa
       61 aggaaaaaat cgttactatt ctcctgattt aaaacaagaa atgattaatc aagtcttaca
      121 tgaaggctgg actaaagata gagtttctct tgaatacggc ctcccaagtc gtacgatact
      181 tcttaactgg ctagcacaat acaggaaaaa cgggtatact attgttgaga aaacaagagg
      241 gagagtacct aaaatgggac gtaagccaaa aaagagacct gaagagagga cagaattaga
      301 atatcttcaa gcagaaaatg agtacctgag agcggagaat gccatcctaa aaaagttaag
      361 agaactccga ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt tcaagaatta
      421 atgactgagt tttcgttaga tcttcttcta aaagccatta aactagctcg ttcgacctac
      481 tactatcact tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt
      541 caatccatct ttatcgaaca caagggaaat tatggttatc gtcggattta tttagaacta
      601 agaaatcgtg gttatctggt aaatcataaa agagttcaag gcttgatgaa ggtactcaat
      661 ttacaagcta aaatgcgaca gaaacgaaaa taaaggagac gttggtaaga aggcagagaa
      721 tctcattcaa ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac
      781 agaatttgcc attccagcaa gtactcaaaa gctttactta tcaccagttt tag
//
LOCUS       DAVLTM010000237          816 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVLTM010000237 DAVLTM010000000
VERSION     DAVLTM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.238"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..203
                     /locus_tag="VYN16_002154"
     CDS             <1..203
                     /locus_tag="VYN16_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV1404581.1"
                     /translation="SQNLDDFNVFSKMSFLEAMEYAMNFNSRRLDTLDKSDWLYDIYK
                     KAFNYKYDTFSELKKDFIEVLK"
     gene            217..>816
                     /locus_tag="VYN16_002155"
     CDS             217..>816
                     /locus_tag="VYN16_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1404582.1"
                     /translation="MNCFLKMNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKS
                     TLINSIVGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLN
                     LAGKFGKNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDE
                     PTVGLDTESAEKFLMYLKDKSLEGKTIIISSHDINLLEKF"
BASE COUNT          285 a           91 c          157 g          283 t
ORIGIN      
        1 ggagtcagaa tttagatgat tttaacgtct tttcaaaaat gagtttttta gaagcgatgg
       61 agtatgcaat gaattttaat tcgcgtcgct tagacacact tgataaatca gattggcttt
      121 atgatattta taaaaaagcg ttcaattata aatatgatac tttttctgaa ttaaaaaaag
      181 attttataga ggtattaaaa taaaggtagg ggtgttatga attgtttctt gaaaatgaat
      241 aatgtaagtg ttcgttatga tgacgtaata gctttaaaag atataacttt acaaataaat
      301 aagggagatt tcattggctt attaggttca aatggtgcag gtaaatctac gttaattaat
      361 tctattgtag gttttcaaga gatttattta ggagaaatag agtattgtga taaagatttg
      421 atagttagtt ctcaaccttt tgctcattta ggctttactc ctcaaaccac agtaattgat
      481 ttttatacta ctgtgaagga caatgtaata ttggggctga accttgctgg aaagtttggg
      541 aaaaatgctg agaagttgtg tcaaatagcc ttagaaattg ttgggttagc tgataaaaaa
      601 aataatttgg tagaaacatt gtcaggtgga caactgcaac gcgtccagat tgctagagca
      661 atagctcata atccagattt ttatatttta gatgaaccta ccgttggttt agatactgaa
      721 tctgccgaaa aatttttaat gtatttaaaa gataagagtt tggaaggaaa aactattatc
      781 atatcttcac atgacataaa tctactcgaa aagttt
//
LOCUS       DAVLTM010000238          799 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVLTM010000238 DAVLTM010000000
VERSION     DAVLTM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.239"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..799
                     /locus_tag="VYN16_002156"
                     /pseudo
     CDS             1..799
                     /locus_tag="VYN16_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          301 a          142 c          160 g          196 t
ORIGIN      
        1 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
       61 agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa gttaagagaa
      121 ctccgattga aggaggaaaa agagaaagaa gaaagacaga aattgttcaa gaattaatga
      181 ctgagttttc gttagctctt cttctaaaag ccattaaact agctcgttcg atctactact
      241 atcacttgaa acagctagat aaaccagata aggaccaaga gcttaaagct gaaattcaat
      301 ccatctttat cgaacacaag ggaaattatg gttatcgtcg gatttattta gaactaagaa
      361 atcgtggtta tctggtaaat cataaaagag ttcaaggctt gatgaaagta ctcaatttac
      421 aagctaaaat gcgacagaaa cgaaaataaa ggagacgttg gtaagaaggc agagaatctc
      481 attcaaggcc aatttgaagg atctaaaaca atggaacagt gctacacaga tatgacagaa
      541 tttgccattc cagcaagtac tcaaaagctt tacttatcac cagttttaga tggctttaat
      601 agcgaaatta tcgcctataa tctttcaact tcacccaact tagaacaagt acaaacaatg
      661 ttggaacagg cattcacaga gaagcactac gagaatacga ttctccatag tgaccaaggc
      721 tggcaatacc aacacgattc ttatcatcgg ttcttagaga gtaagggagt tcaagcatct
      781 atgtcacgca agggtaata
//
LOCUS       DAVLTM010000239          795 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVLTM010000239 DAVLTM010000000
VERSION     DAVLTM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.240"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..233
                     /locus_tag="VYN16_002157"
     CDS             <1..233
                     /locus_tag="VYN16_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404583.1"
                     /translation="AVSQEELEKLEKSYDEKSHEELVSIESNLEMKQQNLIDEVNKTI
                     KENDANIQYISSSRRGEFVEKIIGRVVEKYGH"
     gene            223..>795
                     /locus_tag="VYN16_002158"
     CDS             223..>795
                     /locus_tag="VYN16_002158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1404584.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRL"
BASE COUNT          322 a           99 c          150 g          224 t
ORIGIN      
        1 aagctgtttc ccaagaagaa ttggaaaaac tagaaaaaag ttatgatgaa aaaagtcacg
       61 aagaattagt ttcaatagaa agcaatttag aaatgaaaca acagaacctt attgatgagg
      121 ttaataaaac aatcaaggaa aatgatgcaa atattcagta tatttcatca agtaggagag
      181 gagaatttgt agaaaaaatt attggtaggg tggtagaaaa atatggccat tagtcagatg
      241 aaaagaatct ctctactatt ttctaaaagt agtcttgatg atgttttaaa aactattcaa
      301 gaactagagt cagtgcagtt ccgtgattta aaggttcagg ataattggtc agaagctcta
      361 gaaaaagatg aagttgtatt tccaactatt caaatttttc atacttctaa ttccaatcat
      421 ggggttattg agggaaatga tgccttgact tatttgatga atcaacaaca acatttagaa
      481 gcaactgtag agaaattaca agaataccta ccgaaagaaa acacgtttaa attattgcag
      541 caacctccga taactacctc ttatgaagaa ttagagaaat ttggtaaagc taatgttgct
      601 gagggtgttc ttaaaaaagt gaatcatcaa attaacagag ttcatgaatt agaaagacac
      661 attcaaagta ataatgagga aatagagcga ttaataaagt gggaaaaatt agaaattgtt
      721 cctgcgaatt tagaacaatt ttctttctgt aaaggaaaag tcggaacaat tccaaggact
      781 gaagataatc gctta
//
LOCUS       DAVLTM010000240          733 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVLTM010000240 DAVLTM010000000
VERSION     DAVLTM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.241"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
BASE COUNT          242 a           95 c          125 g          271 t
ORIGIN      
        1 atcagtattg tttgaaatcc aaaaaaagta tgttaagcta tatttatttg gttctttagt
       61 ttgattgttg ttgagtataa ctatttttgt atagatgaaa atcttgttat tgttcaagat
      121 gttttgatga ccattcatga tttgagtaat ccaaggtcaa aatgagtgct atactagcag
      181 tgtaaagatt cttgttcaac taagataaat cacacattta ggaggaaatt ttatgaagaa
      241 aaaaatactt atcattttca tcttgtatct aatcatgtcc atctttcttt atccgcttag
      301 ggaaagtatt tggtatcagc tattttatac catagcctat gtgattgcag ttatgatcta
      361 ttttgcttta actaaaaaga aaggagcaaa gaaatgaaaa cttttcttgc taaaaaacgg
      421 aacatcttcc tttcatttgg ggattgaagt attataaaaa gtagagctta ataaagtata
      481 aaggggtaag tgtttttgaa atacttaccc ttttataata gattccctgc gaaacaaaat
      541 atggtatagt atagtagatt gaaactagaa tagtacgtct ctacttctaa aacattgtta
      601 gaaatcgatt tgactgtcct gatcgattta tcctgttctt atttcatttt actatagttc
      661 tatgaatgat gaagcaagta aacaactaac tgatgcacga tttaagcgtc ttgttggttg
      721 gtgttcagcg tac
//
LOCUS       DAVLTM010000241          732 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVLTM010000241 DAVLTM010000000
VERSION     DAVLTM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.242"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            1..724
                     /locus_tag="VYN16_002159"
                     /pseudo
     CDS             1..724
                     /locus_tag="VYN16_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          261 a           65 c          156 g          250 t
ORIGIN      
        1 ccatggatgg gttgaatata acttaaaaaa gaaattcttg acttattttg atttttggac
       61 atattctatg ggagatgctt ttatttgtgt atctgaaact atgaaaaaaa gattggagag
      121 tataataaaa aaataagaag atagttgcaa taaataacgg aattaatgta tcaaatagcg
      181 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
      241 aaatcatttg ttatttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt
      301 tgcagaaaaa ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag
      361 tatgtgggct gctttgaaag aaaaaatttg ccatttgaaa tgtagagata atttcaggtt
      421 acttggtgaa atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc
      481 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt
      541 tttagctagt aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt
      601 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
      661 tgagtatttg aaatatatag gtgagaatag taaatcaaaa ataagaaaac atgagagtat
      721 atgatgaatt at
//
LOCUS       DAVLTM010000242          712 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVLTM010000242 DAVLTM010000000
VERSION     DAVLTM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.243"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..481)
                     /locus_tag="VYN16_002160"
     CDS             complement(<1..481)
                     /locus_tag="VYN16_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1404585.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     "
     gene            complement(483..>712)
                     /locus_tag="VYN16_002161"
     CDS             complement(483..>712)
                     /locus_tag="VYN16_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1404586.1"
                     /translation="VVVLNFNSLLSDYDLSVFLYHPLFQPLGIVIKQSTDETATLNAQ
                     AMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
BASE COUNT          253 a          145 c          111 g          203 t
ORIGIN      
        1 aaccttctaa agaactaaaa accaagtagt aatcagcaat tttagttttc gagtttaaat
       61 aatctttaag aggagtaaat agagttaaaa ctgctatgag tacaactccg gctaaaatag
      121 caccttcata aaaaccaatg cctatagcta aaccaatagt tgctgaagac cataatccag
      181 ctgctgttgt taaaccttta actttttgat tagttaccat gattgttcct gcccctagga
      241 aacccaagcc actaattact tgggctccca ttcgactagg atcaccgcta ccataacgac
      301 tagtgatgaa ctgatttgtc atcattacaa cacaagttcc caaacaaact agtaagtagg
      361 ttctaatccc tgctgcttgg tttttgactc ctcgctcata gccaacaatg ccaccgaaaa
      421 gaatcgctaa aaagcaccta agaagtattt cccaaatact cagttcgtat gaaagattca
      481 tattatctct tacctcgttt accttggaat aggcttgata aataaagagc tgcactagac
      541 ataatcatta aaattacaga ataaacaaac atcattgcct gtgcatttaa agttgctgtt
      601 tcatcagtag actgtttaat aacgattccc aatggttgga aaagtggatg gtacaagaat
      661 acagataagt catagtcaga taataaagaa ttaaagttta acacaactac tg
//
LOCUS       DAVLTM010000243          712 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVLTM010000243 DAVLTM010000000
VERSION     DAVLTM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.244"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            232..606
                     /locus_tag="VYN16_002162"
     CDS             232..606
                     /locus_tag="VYN16_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1404587.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLGRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          239 a           89 c          149 g          235 t
ORIGIN      
        1 caaaaaaaat aaattttgaa atagtattag tgagttcgca atcatttatt gctagtggtt
       61 tagaactgtt aagtttcaaa caataattga ttttaaacct taggtaacct ataaaaacaa
      121 ataaggtaga gaaaggatat cttatctagg tagctaagtg ataaaaacac agtcgtctat
      181 cacttgttag gaaactatcc taactatgct taataaaaaa ggagaaaata aatggaactc
      241 gtattaccaa ataattatgt tgctcttgag caagaagaga tgatgtatct tgatggggga
      301 ttttctattc tgagatggcc tgttgcaaca gccattaata tagcttttaa tggtgtttta
      361 ggtggaggag caatcagtct agttagaaat tatattcgta attatggttt ggggcgagtt
      421 acaagcgcaa ttgctggagc agctgcaaga tatgttgggg tacgagttgc aaatagagtg
      481 gcaggatttg cactgtctgc tattaatgga tttgcagctt ggatgtcaat tggcgatgct
      541 attacaacaa tctgggccaa caatgatgta aataggagag acccaaattt aaacgccttg
      601 tggtaactag atatgggtat taaaatggaa aaaatttttg ttattatttt ttttgtatgt
      661 ctatttatat catccataac ttttttagcc tatgattttg ttagcgaaga aa
//
LOCUS       DAVLTM010000244          709 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVLTM010000244 DAVLTM010000000
VERSION     DAVLTM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.245"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            341..640
                     /locus_tag="VYN16_002163"
     CDS             341..640
                     /locus_tag="VYN16_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1404588.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          273 a          131 c          114 g          191 t
ORIGIN      
        1 attttactat tagcataaaa ataataatag acaactattt atccaaaaat agatagaggt
       61 aacatgtttg caaacaaagc atacgaatct ttagtaaaat catttccatg aaactagaat
      121 agagcccctc ttagcaaaaa tcattatttt aatttatttc taatcactcc ttgacataaa
      181 taactctcac caataaaaga ctatgtctta aaaaaatggt ataataaaat caatacttgg
      241 gcttgatggc tatgctacta ataacaatta ggagagaaaa tcaggcactt gttaacaaca
      301 aggattatcc ccttgagatg aaaggaactt tagaaatctt atgatgaaca tgcaaaacat
      361 gatgcgtcaa gcacaaaaac ttcaaaaaca aatggaacaa agccaagctg aacttgctgc
      421 tatgcaattt gttggcaaat ctgctcaaga tcttgtccaa gcgaccttaa ctggcgataa
      481 gaaagttgtc agcattgatt tcaatccagc tgtcgttgac ccagaggacc ttgagactct
      541 ttctgatatg accgttcaag ccatcaactc tgctcttgaa caaatcgatg aaactaccaa
      601 gaaaaaactg ggtgctttcg ctgggaaatt acctttctaa aaacaaggag ctagaacaat
      661 gcttgtcgat aacaaaggct aagaaaggtg caaaaatgac tctataata
//
LOCUS       DAVLTM010000245          695 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVLTM010000245 DAVLTM010000000
VERSION     DAVLTM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.246"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            12..>695
                     /locus_tag="VYN16_002164"
     CDS             12..>695
                     /locus_tag="VYN16_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1404589.1"
                     /translation="MPTHNRYESLIRAVKSCLHQSYKNIEVIIIDDNYSNVNLRNKII
                     HQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRIFKLMACF
                     KKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLE
                     LINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADE
                     EYYNYCRKYF"
BASE COUNT          267 a           72 c          128 g          228 t
ORIGIN      
        1 taagtattat aataccaaca cataataggt atgaatctct gattagagct gtgaaaagtt
       61 gtttacatca aagttataaa aatatcgaag ttataattat tgatgataat tatagtaatg
      121 taaatttaag aaataaaatt atccatcaat ttggctatac caatcataga attaagttaa
      181 ttttaagtaa tgaagattta ggtgcaacta atgccagaaa cataggtatc aaaaattcta
      241 gaggtaagta tatatcattt ttagacgatg atgatgaata tatgccagat cgaattttta
      301 agttgatggc ttgttttaaa aagagtagaa tgaagaattt agctttagtt tatagttatg
      361 gaataataat ttatccaaat ggtacacgag aagaggagaa gaccgatttt gttggaaatc
      421 ccttgtttgt tcaaatggtt cacaatatag caggtacgtc attttggttg tgtaaaaaag
      481 aggtgctaga attaattaat ggttttgaga aaatagattc acatcaggac ggtgttgttt
      541 tattaaaact acttgctcaa ggataccaaa ttgatatagt gcgagaattc ttggtgaatt
      601 actacgctca cagtaaagaa aacggtatca ctggagtgac acaaaaaaca attaatgcag
      661 atgaagaata ttataattac tgtaggaaat atttt
//
LOCUS       DAVLTM010000246          690 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVLTM010000246 DAVLTM010000000
VERSION     DAVLTM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.247"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            26..175
                     /gene="blpO"
                     /locus_tag="VYN16_002165"
     CDS             26..175
                     /gene="blpO"
                     /locus_tag="VYN16_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1404590.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCTAGVAYGAIDG
                     CATTV"
     gene            279..398
                     /locus_tag="VYN16_002166"
     CDS             279..398
                     /locus_tag="VYN16_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1404591.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
BASE COUNT          227 a           82 c          147 g          234 t
ORIGIN      
        1 ccgtaactga aaagtgatat actatatgga tacaaaaatg atgtcacaat ttgcagttat
       61 ggataatgaa atgcttgctt gcgttgaagg tggagatatt gattggggaa gaaaaattag
      121 ttgtacagca ggggttgcat atggcgcaat tgatgggtgt gcaacaacgg tttgacattt
      181 ctattgggac catttgctat aggaataggt gtaactggtg ctgcaggtgg agctatactt
      241 ggaggtgtag cctatgcagc aatatgttgg tggtaattat ggattttaaa agttttatta
      301 ttggtttagt agttggtata tttggtcctt atatggatga cttaattaga aaaatatttt
      361 caaaatcttc caaaaaggat acggatagta ctctctaaaa aataaatttc tcccctgtat
      421 ttgctagctt aatttacagg ggagtttctt taatattgtt tcgcctgtca aaatgatatg
      481 tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa tacaatataa agaggaaagt
      541 atgtttttaa tgcttacttt ttaaattttt taacaattca agatgtttcg atgacaattc
      601 aagatttgga tgaaaaattt taaaaaacaa tgatatacta aacttgtcaa agttgcaacg
      661 ggtgctgcta cattatgtta aaaatcgctc
//
LOCUS       DAVLTM010000247          624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVLTM010000247 DAVLTM010000000
VERSION     DAVLTM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.248"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..467)
                     /locus_tag="VYN16_002167"
     CDS             complement(<1..467)
                     /locus_tag="VYN16_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1404592.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVT"
BASE COUNT          203 a           97 c           98 g          226 t
ORIGIN      
        1 gtcacttttt tatgttttat agaattataa tgttttaggt ctgtgtagat aaaatccaca
       61 tattgaatta aatcgataaa tttttcatga tcaacaaagg cagtagtttc aatggcagtg
      121 tgtatgtgat gttctttagc tgattttaag atggctttag caaattcaaa ctgagcaaat
      181 atttcacctc ctgataaagt taaacctcca ccggattctt cgtaaaattc tttgtctttt
      241 aatacctctg taataatttc ttctacagtc ttttcttcac cgactaaggt gaatttctct
      301 cgttgagcat ctttcatttt ttcaggtttc attctttgag attcaggatt agaacaccat
      361 ggacagcgca gaggacatcc ttttaaaaaa acagttgtac gaatacccgg gccgtcatga
      421 attgaaaagt gttgaatatt aaaaataatt cctttagata tttccataag agttcctcct
      481 cttcataagt ctatcctatc acaaaaacga aagaaaaaca attacgaacg aaagtttaat
      541 ttgttgtttt ttgcttttta ttttggtaga ataaaacgga ggtgtaaaat gaaaagattg
      601 gaacaaatta ttaaattagt atca
//
LOCUS       DAVLTM010000248          599 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVLTM010000248 DAVLTM010000000
VERSION     DAVLTM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.249"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..>599
                     /locus_tag="VYN16_002168"
     CDS             <1..>599
                     /locus_tag="VYN16_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV1404593.1"
                     /translation="VRLKNNKILFINFNGKGYGDNPKSICEYLRTTYPELDLVWLTKE
                     TKGFPDGVRLVKYGSIRAFYEQASSKVWVYNVRAFARILKRKEQFYIQTWHGASSFKL
                     IEKQAELPLNYVLEAKYDARVTDLMISDSKKQTEEYRKYFWYTGEILEVGMPRNDSLF
                     HCKDDYNKLENVRKELNISCDDFVILYAPTFRDNGDISY"
BASE COUNT          214 a           72 c          123 g          190 t
ORIGIN      
        1 tgtcaggtta aaaaataata agattctatt tattaatttc aatgggaagg gttatggtga
       61 taatcctaaa agtatttgcg agtacctaag aactacatat ccagaattag atttagtatg
      121 gctcactaaa gaaacaaaag ggtttccaga tggagtcaga ctagtaaaat atggttcaat
      181 tcgagctttt tacgaacaag ctagttcaaa agtatgggtg tataatgtga gggcgtttgc
      241 taggatacta aaaagaaaag agcaatttta tattcagact tggcatggtg cttcaagttt
      301 taaactgata gaaaaacagg ctgaattacc tttgaactat gttctggaag ctaaatatga
      361 tgcacgagtg acggatttaa tgatttcaga cagtaagaag caaactgagg aatacagaaa
      421 atatttttgg tatactggtg aaattcttga ggtagggatg ccaagaaatg attccttgtt
      481 tcattgtaaa gacgattata ataagttaga aaatgttcga aaagaactta atatctcctg
      541 tgatgatttt gtcattttat atgcaccaac ttttagagat aatggtgata tatcctatt
//
LOCUS       DAVLTM010000249          598 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVLTM010000249 DAVLTM010000000
VERSION     DAVLTM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.250"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..>598)
                     /locus_tag="VYN16_002169"
     CDS             complement(<1..>598)
                     /locus_tag="VYN16_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1404594.1"
                     /translation="SCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIR
                     NHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRV
                     QIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQ
                     DLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLF"
BASE COUNT          191 a          106 c          121 g          180 t
ORIGIN      
        1 gaaaaagagg atgaacctgc tttagattgt cctcaatgag tccgaaaaat ttctccggtt
       61 ccttattctg aaagtgaaac agcaagagtt gatagagctg atagtggtgt ttcaagtctt
      121 gtgaatagct caaaagcttg tttaaaatct ctttattggt taaatgcata cgaaaagtag
      181 ggcgataaaa atgtttatcg ctgagtttac gactatcctg ttgtatgagc ttccagtagc
      241 gcttgatagc cttgtattca tgagactttc gatccaattg attcatgatt tgaacacgca
      301 cacgactcat agcacggcta agatgttgta caatgtgaaa gcgatccaac acgattttag
      361 cacatgggaa aagttgtcta gctaagtcat agtaaggact aaacatatcc atagtaataa
      421 ttttgacgcg acatcggacg gctctatcat atttaagaaa gtgatttcga atgatagctt
      481 gtgttcttcc ctcaagaaca gtgatgatat tgagattgtt aaaatcttgc gcaatgaagc
      541 tcatctttcc ctttgtaaaa gcatactcat cccaagacat aatctcagga agacaaga
//
LOCUS       DAVLTM010000250          578 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVLTM010000250 DAVLTM010000000
VERSION     DAVLTM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.251"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..303)
                     /locus_tag="VYN16_002170"
     CDS             complement(<1..303)
                     /locus_tag="VYN16_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV1404595.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAV"
     gene            complement(293..>578)
                     /locus_tag="VYN16_002171"
     CDS             complement(293..>578)
                     /locus_tag="VYN16_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV1404596.1"
                     /translation="FNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQG
                     RYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
BASE COUNT          190 a          100 c           83 g          205 t
ORIGIN      
        1 tactgctttc tctacattat cctctatacg ttttaatcct aaatttaaac cttgtttatg
       61 agtcggtaat aaaataatag caggcacaac ttgggaatca taatggcgaa ttgtatctaa
      121 tatcattgaa gcaatgtctt cagtgatata aatgacacca taatcagatt gagctaattt
      181 tcttagtgta tttatagctt cttgttcttg gtaggcagga aatatatcaa acccaatcat
      241 gctaaatggt aaaataatat cacggctacc aattatgcca attttatact tattagcttc
      301 cataatgtgg gcgtatcctt tcttttattg attcaaaggg aattgtattt tgaagagctg
      361 atatgatcat tctgagattc tttacttcaa actcacatcc caataaatat ctagcaagaa
      421 ctaacggccc ctctacattg taccttcctt gatctaaagt tttatacaat agtaaatcac
      481 ataaaaattc taactcagaa gatgaaattg ttgcgtcctg catcttcaat tcataagttg
      541 aaaagattga atctaagctt ggatttattt tattgaac
//
LOCUS       DAVLTM010000251          575 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVLTM010000251 DAVLTM010000000
VERSION     DAVLTM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.252"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
BASE COUNT          219 a          107 c           64 g          185 t
ORIGIN      
        1 ttattttagt atatccaatc actagtccaa taattggact agcagacatg cttagatata
       61 ataaaaataa caaaaaatga atttcaccaa ttcctgcaaa aaaatgtttc atagctatag
      121 ttttacaccc ataaataccc atcaaattta ccgtctacga tatctaaacc atatgcaatg
      181 gctcctccta atgagaagac ttcagcaaca gtagccgcaa tattaagagc attagtaata
      241 gttgccatac taactccacc aatatatctt gttataacac ttagttacct agataagata
      301 tcctttagct tgcttccaaa atttaagtct gcacctataa tatgagttca tattaatact
      361 gctaaatcct atctatagta atgtcaaaca cccaaataaa gtcatctctc caattcattc
      421 aagaaatgaa gaaaggcaaa aaatctttaa aataagaaaa atgcatatta tcaggtgttc
      481 aaaaatattg atgatatacg ttatatagat agaaagattt actcaatctc attttcaaat
      541 tgagtttttt cccagcctcg tttttaattt atatc
//
LOCUS       DAVLTM010000252          556 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVLTM010000252 DAVLTM010000000
VERSION     DAVLTM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.253"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(323..>556)
                     /locus_tag="VYN16_002172"
     CDS             complement(323..>556)
                     /locus_tag="VYN16_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404597.1"
                     /translation="DNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEF
                     IACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          194 a          103 c           78 g          181 t
ORIGIN      
        1 caaaatatag aataacggta tcgccaatac aaaggttaag atggcatgag agggagttcc
       61 taagatgtcc aattttataa atactgggct gacacaaaat atcgtgctta taagcagtaa
      121 aattattcca atctccccca tcaagatagc ttatttatcc tagacaacgc ctactgcaac
      181 agtataaata agattcatca gccatctttt ctggcaatca tatgggtaga ctaatgatat
      241 ttctagttac tcaaaaatat ctagctatac tagagttatt tctaatccct aggacacttg
      301 ttaatccaaa tgtaacaaat tttcacccct taataggact ctttctggaa aaaatataag
      361 gtctatcttt ataaaaaaat ctatcatatt gggatggcac gcatctggaa tttaaacaag
      421 ctataaattc atttataact actatttgtt tagaggcaac agcatcttgt ataccgccct
      481 cgaaagaacc atcttgtaat tccgaaatat ttttgtttaa atattgtttt gacaaatctc
      541 ttaatgcttc attatc
//
LOCUS       DAVLTM010000253          509 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVLTM010000253 DAVLTM010000000
VERSION     DAVLTM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.254"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..>509
                     /locus_tag="VYN16_002173"
     CDS             <1..>509
                     /locus_tag="VYN16_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1404598.1"
                     /translation="ARLLDDRNYDNLEYIYRIEIERSTFLTLEDRTYLEWIKAIIDFY
                     QYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFYSLVGREQEYEANYSHLIELYQ
                     TKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETIELCKQRQTSYQLAPLLI
                     LVGNAGAKF"
BASE COUNT          179 a           83 c           84 g          163 t
ORIGIN      
        1 tgctcgacta ttagatgaca gaaattatga taatttggaa tatatttata gaattgagat
       61 tgaacgaagt acttttctaa cactagaaga ccgaacttat cttgaatgga ttaaagctat
      121 tattgacttc tatcaatatg acagtaagtg tgaggcaatt tcttcattgg aaaatatatt
      181 attaaaagtc tcttcaaata ctctgattta tttaaaggcg ttgaatactc tatctaattt
      241 ctattcctta gtgggtcgtg aacaagaata tgaggcaaac tactctcatt taatagagtt
      301 gtatcagaca aaaaatttgg accatcaaga gtttttattt ggctacatca gagttcgtta
      361 caactacgct cactacctag tatcaaagga aaaatataat gaagccatcc aagaagctct
      421 tgaaacgatt gaactctgta aacaaagaca gacaagctac caattggctc ctctacttat
      481 tcttgtagga aatgctggag ccaaatttc
//
LOCUS       DAVLTM010000254          508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVLTM010000254 DAVLTM010000000
VERSION     DAVLTM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.255"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..460)
                     /locus_tag="VYN16_002174"
     CDS             complement(<1..460)
                     /locus_tag="VYN16_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404599.1"
                     /translation="MVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREISLLLATII
                     VYFFTHRILPFSVFTFMFSYILLFVQSYLGGNTAWIGNRRLIIDDEFEKILLSKSYIK
                     EISSARYSEYLTCEYKNFTPIILLAIFENLLDSYLLQNQSEVDLDIFYKVL"
BASE COUNT          182 a           83 c           77 g          166 t
ORIGIN      
        1 gtaaaacttt ataaaaaata tctaagtcaa cctcactctg attctgaagc aagtaggagt
       61 caagcaaatt ttcaaaaata gctaacaata tgattggtgt aaagtttttg tattcacatg
      121 tcaagtattc tgagtatcgt gcagatgata tttcttttat ataacttttt gaaagcaaga
      181 ttttttcaaa ttcatcatct attattaatc ttctatttcc aatccaagcg gtattaccac
      241 ccaaataaga ttgtacaaat agcaatatat aagaaaacat gaacgtaaat actgaaaaag
      301 gtagaattct atgcgtaaaa aaatagacga taatggtagc caggagtaaa gatatctctc
      361 gtgacaatcg aattcttttc agcatctttt ttaacagtcc gtaaggttgt ccttggcaat
      421 attcttgaac taaattaatc ggaatgacat ctctcaccat ttctggaaag tagagtaagt
      481 ttataggttg aaatctccag ctcccatc
//
LOCUS       DAVLTM010000255          500 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVLTM010000255 DAVLTM010000000
VERSION     DAVLTM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.256"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            233..484
                     /locus_tag="VYN16_002175"
     CDS             233..484
                     /locus_tag="VYN16_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404600.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQSA"
BASE COUNT          185 a           57 c           90 g          168 t
ORIGIN      
        1 gcgaagtttt tataactagt tcctataagt acagaaacct attttaattt actaatttca
       61 aaacaatttt ttgagatata tgtaattaaa tctaaattaa gctttaaaaa attattttta
      121 gcaatttgat tttgaaagtg tcatgcagca atatgaaagc gtagaataga ggtgtttcca
      181 atgtacaagt aagttttgat tccaaataaa gtaatagaaa aggagctata taatgagtag
      241 tcaaatgaaa gctttcttga atgaacttca aggaaatatg gaagttgttg gagaattttt
      301 tgataatcct gaaaaggtaa ttagaaaatt tggaattgtc ggaagagaga aggaagcatt
      361 gctaatccgt gacttaaatg atatggatga ttatgctctt tcaattcaaa atagcgttgc
      421 atcgccttct ggggcacata gtagcacttg ccattttgtt agacaagatt tacaatccgc
      481 ataattgtta atatataaaa
//
LOCUS       DAVLTM010000256          498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVLTM010000256 DAVLTM010000000
VERSION     DAVLTM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.257"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(214..498)
                     /locus_tag="VYN16_002176"
                     /pseudo
     CDS             complement(214..498)
                     /locus_tag="VYN16_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          177 a           83 c           80 g          158 t
ORIGIN      
        1 taaacttatg taataaaaca ttgaggaaat aataaaaatc tatgggtaat gttctgaaag
       61 tacttttagg ttaggagaga aatataatga tttttgaaaa ttgattcaaa aaatagcgaa
      121 ttgtagaaaa cgcttaacaa atggtgtata atagacttcc agcgaaacaa aatatgctac
      181 aataaaccta tgaattataa agcaacctta tactcatctg tcttcacaaa gcattctagt
      241 ccatctccga ttaacgatgg actttatcat ctccttctcc agtccttgta tgacatcttc
      301 caaagttcga aaggctttat tcttaaatcc acgtttacga atctctttcc acacttattc
      361 aatggggttc atctctggtg tgtatggagg aataaatgca aagccaatat tagtcggaat
      421 ctttaaggca cttgatttat gccatatagc attgtccata acgagtaaaa tataatcatc
      481 tggataagct tgtgaaag
//
LOCUS       DAVLTM010000257          478 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVLTM010000257 DAVLTM010000000
VERSION     DAVLTM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.258"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
BASE COUNT          131 a          109 c           64 g          174 t
ORIGIN      
        1 taaacaaaaa aacaagtcta tatttcggtt tgccgataac ttttttatat catacccgct
       61 tgttttattc catggtctac ttgaccgcaa gtaacctagg tcgacgtact ttgtccgtgc
      121 cgctccgaaa cgctacagcc tttcggctcg tccctgacta gcgtactttg atctagctta
      181 cggtcaagta gctttcacgg cataaatcgc tcagaaatta caatatactt tctactaaaa
      241 ataccccaaa aatactacct tgatacaatc tgaaactaca tttttcagtc gagtattttc
      301 gtttctaaaa aaagataaat ctctttttag gctctccatt tcgtttttaa gctcatcttt
      361 ggtttcctgt aattctaagg gttttatcct ctaaaaaatc aattctggag ctcctgacgg
      421 ctattaattc atctattctc tttaattcct cttttgcttg attaacattt tccataat
//
LOCUS       DAVLTM010000258          471 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVLTM010000258 DAVLTM010000000
VERSION     DAVLTM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.259"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            <1..>471
                     /locus_tag="VYN16_002177"
     CDS             <1..>471
                     /locus_tag="VYN16_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404601.1"
                     /translation="KKYKWAKVKGDKGEKGDKGERGLQGLQGLQGARGEQGIPGPRGA
                     DGRTQYTHMAYADNATGGGFSQTNTDKAFVGVYIDFNPTDSRNPADYRWTRWKGRDGA
                     NGVAGRAGADGRTPYLHIAYATSNNGSQGFSTTDSTNKTYIGTYTDYTQADSTDP"
BASE COUNT          163 a           97 c          120 g           91 t
ORIGIN      
        1 aaaaaataca agtgggctaa agtaaaaggg gacaagggag aaaaaggcga taaaggagaa
       61 cgtggattac aaggtttaca aggcttgcaa ggcgcacgag gtgaacaagg tattcctgga
      121 cctagagggg cagatggccg tacacaatat actcacatgg cctatgccga taacgcaaca
      181 ggtggtggat tcagtcaaac aaacactgac aaagcctttg ttggagtgta cattgacttt
      241 aatccaacag acagcagaaa tcctgctgat tatcgctgga caagatggaa aggtcgtgat
      301 ggcgcaaatg gcgtggcagg tagggctggt gcagatggta ggacaccata cttacacata
      361 gcttacgcca catcaaataa cggctcacaa ggcttctcaa ctactgacag tacaaataaa
      421 acgtatatcg gaacatacac agattacact caggcagata gtaccgaccc t
//
LOCUS       DAVLTM010000259          471 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVLTM010000259 DAVLTM010000000
VERSION     DAVLTM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.260"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            31..441
                     /locus_tag="VYN16_002178"
     CDS             31..441
                     /locus_tag="VYN16_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404602.1"
                     /translation="MIQEKERKIQELNKEDFLDKLEKTLLKNHYDELNGLSFNIILKA
                     SIGSVIEESYRNTKHYPIDKWQKLRQQMERDVKNVNPNLETTVTPRIYLDEDVLAGLD
                     DFRYVLMKEDCATRLPRLSYIIKLVVYSYWKEQH"
BASE COUNT          170 a           78 c           84 g          139 t
ORIGIN      
        1 gcgtacgagg caaaggtatg gatagatgaa ttgattcaag aaaaagaacg taaaattcaa
       61 gaacttaata aagaggattt tctagataaa cttgaaaaaa cacttcttaa aaatcactat
      121 gatgaattga atggtctttc atttaatatc attctaaaag cctcaatagg gagtgtcata
      181 gaagaatcct atcgaaatac taaacactat ccaattgaca aatggcaaaa gctacgtcag
      241 cagatggaaa gggacgtaaa aaatgttaat cctaacttag aaaccacagt tactccccgt
      301 atttatctag acgaggatgt gcttgctggg cttgatgatt ttcgctatgt tttgatgaaa
      361 gaggactgtg ctactagact acctcgtctt agctacatta tcaaacttgt tgtatattcc
      421 tactggaaag aacaacacta atctaatcag ttctactgtt tagattttca t
//
LOCUS       DAVLTM010000260          429 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVLTM010000260 DAVLTM010000000
VERSION     DAVLTM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.261"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            complement(<1..>429)
                     /locus_tag="VYN16_002179"
     CDS             complement(<1..>429)
                     /locus_tag="VYN16_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404603.1"
                     /translation="TTSTYTATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALG
                     QFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIP
                     KGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSY"
BASE COUNT          116 a           95 c           69 g          149 t
ORIGIN      
        1 aataagaagt atctacatcg cttatttcaa acttcggcat ccaaatccaa acagttcctt
       61 cctggttgaa attgaacaac cattcatttg tggtctgctt ggattcgttt gtccaacctt
      121 ttggaatatg gacaacatat cgtttaattt ctgtcgacaa tgtcacattt ccagttttat
      181 atccgatatt ccctaatcga gatcttagca ttattccatt tttatttgcc ttagcataaa
      241 aactaatggt tacatcttga ttagtcgtac ttccgggaat tactttcccg aattgaccca
      301 gagctggata agtaaccttg ggattccctc catcacgacc agatggattc agacctataa
      361 ttttaagagc cttgtgtcca agatacttac tttcgctatc gatagtagcc gtatatgtac
      421 tcgttgtcc
//
LOCUS       DAVLTM010000261          426 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVLTM010000261 DAVLTM010000000
VERSION     DAVLTM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.denovo.262"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            7..210
                     /locus_tag="VYN16_002180"
     CDS             7..210
                     /locus_tag="VYN16_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV1404604.1"
                     /translation="MNTKMMSQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
BASE COUNT          124 a           59 c           93 g          150 t
ORIGIN      
        1 tttgtcatga atacaaaaat gatgtcacaa ttttctgtta tggataatga aatgcttgat
       61 aggatagagg gaggaatatt tggagttgac gatgctttgt tttgggcagg gttaggctat
      121 gttgcaggta gcattgttga tacagcaatt gatgatttta cgaatcaatg ccggaaaaat
      181 cctcatcagt ggttctgtgt tcgtgtatag atatagtagt gttgtattgt ataagaattt
      241 atataactta atgacctatg cagtgataga aactattctt ttgcaaacga gtcgcttatt
      301 ctgttataag tttacgtgct cctagtcaca gttcttgtgc ttgatagaaa gaatgacacc
      361 tataaaaatg cgagatattg gctatatcac tcacttattc acctccccgt gatttgtagt
      421 agtgat
//
LOCUS       DAVLTM010000262        14883 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059720
            SAMN33607345-rid17952493.guided.045, whole genome shotgun sequence.
ACCESSION   DAVLTM010000262 DAVLTM010000000
VERSION     DAVLTM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607345
            Sequence Read Archive: SRR23725644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721521.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721521.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:43:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,196
            CDSs (total)                      :: 2,151
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 77 of 136
            Pseudo Genes (incomplete)         :: 63 of 136
            Pseudo Genes (internal stop)      :: 29 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607345-rid17952493.guided.045"
                     /strain="AUSMDU00059720"
                     /serotype="23B1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1373"
     gene            111..299
                     /locus_tag="VYN16_002181"
     CDS             111..299
                     /locus_tag="VYN16_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1404605.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            361..1119
                     /locus_tag="VYN16_002182"
     CDS             361..1119
                     /locus_tag="VYN16_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1404606.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            1177..1638
                     /locus_tag="VYN16_002183"
     CDS             1177..1638
                     /locus_tag="VYN16_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1404607.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRF"
     gene            1672..2031
                     /locus_tag="VYN16_002184"
     CDS             1672..2031
                     /locus_tag="VYN16_002184"
                     /inference="COORDINATES: protein motif:HMM:NF015692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1404608.1"
                     /translation="MNDNPIECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQET
                     VPQAIIAFLESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLD
                     EPLKDVVRRWENRIKVY"
     gene            2143..3027
                     /locus_tag="VYN16_002185"
     CDS             2143..3027
                     /locus_tag="VYN16_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1404609.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKQGILA
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3190..4929
                     /locus_tag="VYN16_002186"
     CDS             3190..4929
                     /locus_tag="VYN16_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1404610.1"
                     /translation="MELSAIYHRTESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            5204..5506
                     /locus_tag="VYN16_002187"
     CDS             5204..5506
                     /locus_tag="VYN16_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1404611.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            5562..5990
                     /locus_tag="VYN16_002188"
     CDS             5562..5990
                     /locus_tag="VYN16_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1404612.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
     gene            6048..6657
                     /locus_tag="VYN16_002189"
                     /pseudo
     CDS             6048..6657
                     /locus_tag="VYN16_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            6939..7186
                     /locus_tag="VYN16_002190"
                     /pseudo
     CDS             6939..7186
                     /locus_tag="VYN16_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            7291..7593
                     /locus_tag="VYN16_002191"
                     /pseudo
     CDS             7291..7593
                     /locus_tag="VYN16_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            7749..8978
                     /locus_tag="VYN16_002192"
     CDS             7749..8978
                     /locus_tag="VYN16_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1404613.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            8998..11694
                     /gene="ppc"
                     /locus_tag="VYN16_002193"
     CDS             8998..11694
                     /gene="ppc"
                     /locus_tag="VYN16_002193"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1404614.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    11777..11994
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12107..13111
                     /locus_tag="VYN16_002194"
     CDS             12107..13111
                     /locus_tag="VYN16_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1404615.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            13180..14046
                     /locus_tag="VYN16_002195"
     CDS             13180..14046
                     /locus_tag="VYN16_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1404616.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            14043..14801
                     /locus_tag="VYN16_002196"
     CDS             14043..14801
                     /locus_tag="VYN16_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1404617.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
BASE COUNT         4851 a         2350 c         3137 g         4545 t
ORIGIN      
        1 gatttaaagt tactgtgtta aaaatcctct ccacttttaa actaatgtat tataaaagcc
       61 agttgacaca agtgttacac ctgtgataga attaacttga ggtgatgaaa atggcaaccg
      121 ataaaaatag aataatgatt agcttggatg ataaaaatct agagaaactt gaaaatttag
      181 tagaagatgc tagagataga aggggaatgc gactaacaaa gtcccaggtt attgaattac
      241 tcctaaatac cgttgattat ttcgatgata ttatgggggc tatttactca aaaaaataag
      301 agcaaaattg ctattatttt ttacaaattg gtgtaacact agtgatatag gagtgatgaa
      361 atggttgttt atattagaca aagtaaatta ccaagtgagg tttcaattaa taaatacaat
      421 gctcaagtag gtgcctactt acaaggagaa gaagctgttt tatatcaatc tttttctgaa
      481 ataaaagagt taacaagtga agatatagtt gtagattata taatggagac tagagcatta
      541 ctaaaaatga tgggcttaaa cgttccggtt catgactatc ctattgagct taaagagttt
      601 tatggtcgaa agatttatgc tggtatttta ggagagattg tgaatatacc tgataattgg
      661 ggaaaattta ttaagcctaa agctggttca aaagtcttta ctggaagagt tgttaatgga
      721 acccatgatt taataggtat tggtctacct ttcgactatc ctatatggat tagtgaggtt
      781 gtagaattca tagctgaatg gcgttgtttt gtgttagatg gtcgcgtatt agatgttcga
      841 ccctatacag gtgattatca tgcacaattt gatgcaagtg taattgatga agcgatatca
      901 tgttggaaag atgcgccaat agcttatgga ctagatatcg gtgttactcg cgatggcaga
      961 acacttgttg ttgaagtaaa tgatggttat gcattgggaa attatggcct atctccttta
     1021 aaatcaatca attttcatag agctaggtgg aaagaaatgg taaaacccta ttttgaaaaa
     1081 aatgaaattt ttaaaatcca acaggatgtt gttttctaaa ataaatatgg tatataacca
     1141 atataaaaag catcaaaatg aacaaggagg aatctgatgc ttggagcaat tattggagat
     1201 attgtcggtt ctgtttacga atggaacaat atcaaaacaa aggactttcc tttatttcgg
     1261 aaggactgct tttttacaga tgacacggtt atgacctgtg ctgttgcaga agcaattatg
     1321 aatggtggac aaaaagacga cttcattgac gcgatgaaga aatatggcag aatgtatccg
     1381 aatgctgatt acggtgctcg gtttaatgca tggctaaaca gcgataaccg tgagccttat
     1441 aatagctttg ggaatggatc agctatgcgt atttctccat gtgcttggat catggactgt
     1501 ggtttttatg cgaaaactgg tatatggcca tcatctagag gacttacgag tctttctgca
     1561 gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta tggctacagc tgatgctatc
     1621 tttctatgtc gtttttaatt tggtggttat tgtagagagt acgagtaatc aattaacgac
     1681 aatcctatag agtgtaaaag acgaattaag gattatatag aaaaagaata cgattacaat
     1741 ctatctcaaa ctttagatga aatccgtcct aactatcgtt ttaacgaaac atgtcaggaa
     1801 actgtccctc aagccattat cgcctttctt gaaagtagag acttcgaaga tgcgataaga
     1861 aatgccatct cacttggtgg cgacagtgat actctcgcag caatcacttg tagcatagca
     1921 gaggcagctt acggtattcc tgattggatc aaggataagg cctattctta cttggatgaa
     1981 cccttaaagg atgtagttag gcgatgggaa aatagaataa aagtgtatta atctccagta
     2041 cgtcagattt ctcttttctg tattttcatt gtataatagg attacattta cttgcatacg
     2101 acgaaatgta aacatttcta tttaaaatta aggataaaat caatgaaaaa agcaatggta
     2161 attatcaacc ctacttctgg tggcgagaag gctttggatt acaaagaaaa gctggagaat
     2221 aaagcaaaag aatactttga atatgttgaa accaaaatta ccgaaaaagc gctggatgca
     2281 acacattttg ctgaagaagc ttctcgtgag cagtatgatg cagtggttgt gtttggtgga
     2341 gatgggactg tcaatgaagt catttcaggt attgatgaga gagactacat tcctaagtta
     2401 gggattatcc caggcggtac gggtaacctc attacaaaac ttttggaaat caatcaagac
     2461 atcgatggcg caattgacga actggatttt gatttaacca ataagattga tatcggtaaa
     2521 gcaaatgaca actattttgg ttatatcttt agtatcggtt ctctgcctga ggcgattcac
     2581 aatgttgaaa tagaggacaa aacaaaacaa ggtattttag cctatgctgt aaatactatg
     2641 aagtctgtca tgacagatca ggtctttaac attaaggttg agacagaaaa tggaaattat
     2701 gttggtgaag ctagccatgt tttggtcctt ttgacaaatt acttcgctga taagaaaatc
     2761 tttgaagaaa acaaggacgg ctatgccaac attttgattc tgaaagatgc ctctatattc
     2821 tccaaattat ccgtcattcc tgatttatta aaaggggatg ttatcgcaaa tgataatatc
     2881 gagtatatca aagcgcgtaa tattaaaatc tcttcagata gtgaattgga gtcagatgtt
     2941 gacggagata aatcagataa cctacctgta gaaatcaaag tcctagctca gcgagtagaa
     3001 gtattttcaa aaccgaaaga ggattagtat atagagaaag ccttttttaa ggctttttgt
     3061 atactttaaa agatagttcc tttaacaacg gacattcctt gcaaatagtt cacaaaaata
     3121 gtatactgga ttcattgagt ttgaaaacgt ttgcgtaaaa tttgaatgaa tactttagga
     3181 gataaattga tggaattgag tgctatttac cataggactg agtcggagta tgcctatctt
     3241 tataaggata agaaactcca tattcgaatt cgaactaaga aaggggacat tgaaagcatc
     3301 aacttgcact atggggaccc ttttatcttt atggaggagt tttatcagga tacaaaagaa
     3361 atggtcaaga taacttctgg taccttattt gaccattggc aggttgaagt gtcagttgac
     3421 tttgcacgta tccagtatct ttttgagctc agagatacag aaggtcaaaa tattttgtat
     3481 ggcgataaag ggtgtgtgga aaattctcta gaaaatcttc atgcaattgg gaatggattt
     3541 aagttgcctt atcttcatga gattgatgcc tgcaaggttc ctgactgggt ttcaaatacg
     3601 gtatggtatc agatatttcc tgaaagattt gccaatggca atgctctatt aaacccagaa
     3661 gggactttag actgggattc atctgtcaca cctaagagcg atgatttctt tggtggtgat
     3721 ttacagggga ttattgatca tatggattac ttgcaagact tgggtattac tggactatat
     3781 ctttgtccca tctttgaatc tacaagcaat cacaagtaca atacgacaga ttactttgaa
     3841 attgaccgtc attttggaga caaggagacc tttcgggaac tggtggatca agcgcatcat
     3901 cgtggcatga aagtcatgct ggatgcggta tttaatcata ttggttcgca atctcttcaa
     3961 tggaaaaatg tcgtcaaaaa tggtgaacag tctgcttata aggattggtt ccatattcaa
     4021 caattcccag tgacaactga aaagctagtt aataagagag acttacccta tcatgttttt
     4081 ggtttcgagg actatatgcc taagctaaat acagccaatc cagaggtcaa gaattatctt
     4141 ttaaaggttg cgacttattg gattgaagag tttaatatcg atgcttggcg tttggatgtg
     4201 gctaatgaga ttgaccatca gttctggaag gattttcgta aggcagtttt agctaaaaat
     4261 cctgatcttt atatcctagg agaagtctgg catacatctc agccttggct aaatggagat
     4321 gagttccatg ccgtcatgaa ttatccttta tctgatagta tcaaggacta tttcttacga
     4381 ggaattaaga agacagacca gttcatcgat gaaatcaatg gagagtctat gtattacaag
     4441 cagcagattt cagaggtcat gtttaatctc ttggattcac atgatacaga gcgaatcctg
     4501 tggacggcca atgaagatgt tcaactggtt aaatcagcct tagcctttct ctttttacaa
     4561 aaaggaacac cgtgcattta ttacggaacc gagctagcct tgactggagg accagatcca
     4621 gattgtcgtc gttgtatgcc ttgggaacgt gtatcaagtg acaatgatat gctgaacttt
     4681 atgaagaggc tgattaagat tcggaaatac gcgtcagtaa tcatttcgca tggcaagtat
     4741 agccttcaag aaatcaaatc tgatctagta gctctggaat ggaaatacga aggacggatc
     4801 ctcaaagcaa tattcaacca atcaacagaa gattatcttt tagagaaaga agcagtagca
     4861 ctagcaagca attgccaaga attggagaat cagcttgtca tctctccaga tggatttgtg
     4921 attttctaaa aactagttga tgaagattat ggtacatttc atatcttata tagtataata
     4981 aggctagtta ctaaacttgt aaaggagaac ttaaatgaat tgtagaggac acgaaacaag
     5041 acaaagaatt gttagagatt ttgaagttta gcctaaagca catattaagc tgttagcaaa
     5101 tcaacaaaaa catagtgatg caggagcaac tattgaagat gaatattatg tatttatcgc
     5161 tgagagtaaa attgatggca agaaggaagt tattcagtgt tgcatgggtg cggcaaggga
     5221 ttttttagaa ctaattaatc acaaagggct acctcttttt aatccgcttg taggtgattc
     5281 tcatgtaaat aatagacaag aatatgacaa tacagggagt ggaaatttac aacctgaaaa
     5341 gtggaatgaa actgcaaagc agctttataa tgttataatg tggttgatta ttttatggaa
     5401 tgctaagccg gatacacctt tatttaattt taaagacgaa gtaattaagt ataaaacata
     5461 tgagcctttt gaaagcagta taaaattggt atcaatcaat gagtaacaaa gtatcagcat
     5521 gattcgaaac atttacttgt aatttgcgga ggaacatcaa tatggatgga ataacaaaag
     5581 attttataaa aacggctaaa ctaatgaaac aattatggcc ccaattgacc gataaagaag
     5641 ctattgatga agtaaaaaaa tatacgaatg gcaaaaatac tgcaatcttt actgaagttg
     5701 aaggtgacac aattgtaggt ctagcactat gttcactcag atttgattat gttgaaggtt
     5761 gtaaatatag tcctgttgga ttcttagaag ggattattgt tgacgaggaa tatcgtttaa
     5821 aggatattgc taaaaatctc tgtacaaaat gtgaggaatg ggcgaaaaat aaaggatgta
     5881 aggaatttgc aagtgactgt actttaacga atacggattc tataagattt catctcaata
     5941 ttggatttca ggaggcaaat agaattattc attttaagaa aaaattataa tttagaaacg
     6001 tgaataaact tgtttaaact ttatgtatct ttagaaagtg tgttaacttg aaagaacaaa
     6061 tttttttgat gggtgggaat cccccaatga agaaatatag tattgttgat aaaattgtat
     6121 tatcaaccaa gattaaaaga attatcattt ttacagtttt tcgagaaaat tgggaaccgt
     6181 acatgaaaaa gtacacggaa gtttttcaaa gtcaatttcc taatctaaac attgattact
     6241 tgctcctgga cactgagcag attgatcttg atagctatct agatgctgac ataattatca
     6301 tcggtggtgg aaatacggaa aaatatattg ctaacttatg ttaatcagga gttcaaaagt
     6361 tatatcgatc atatgcttaa taaagaggca aaaattatag gattttctgc aggagcccta
     6421 ctattaggag aaaaagtcta tgtctcacct aatgataatt cagatcatca gataaagata
     6481 aaaaatggat taggactctt tagtcagttt ttaattagtg tccattatga ttcctggaat
     6541 gataaagcaa ataaggatag agctgaagaa ctcgttaatg ttcccataat tccactaaat
     6601 gatcattctt gtcttgtatt ggataaactt ggaaacatca tagagaaaat tgactaactt
     6661 tcataatgta aaaatggatc taggaaataa aagtgtagac gaactggata ggttactagt
     6721 gaaaactgca aaaagtgttg taaagtgaat cggagtgtgc ttgatggatt ttagtagtaa
     6781 gatagccata aataagggct ggtcagatga taaaaaatat tgtgtgacag atcaaaatca
     6841 gtaaaaatat ttcttgcgtg tttctgataa gtcgaagtta gattataaaa aaatttgaat
     6901 ttgatatgat ggagaaagta gcttctcttg gagttccgat gtgtaaacca attagcattg
     6961 aactctgcga tgacgaagta cattctttac acgaatggat agacggaaga gatgcaatag
     7021 atagcatttt agcttattca gaaaaccaac aatacacata tggtgtagaa gcaggaaaga
     7081 tacttagaaa gattcataca attcctgcta cagaagtttg tgaagattgg gaaatttttt
     7141 taatctaaaa attgatgata aaatctccaa cgaaatgata tggtaagtcc gaaacgctca
     7201 gtgtgtaagc ttgttgctaa aagcgtaaaa accttggaac gaaaggaata atagacttcc
     7261 tgcgaaacaa aaatacaata caataaaact atggatgatg aagcaagtaa acaattaagc
     7321 gatagccgtt tcaagatcct tgtaggtgtt cagcgcacga cttttgaaga gatgttagct
     7381 gtgttaaaaa cagcttatca acgtaaacgc gcaaaaggtg gacgaaaaag caaattaagc
     7441 ctagacgatc tccttatggt aactattcaa tacatgcgag aatagagcac ttatgaacaa
     7501 attgcggctg attttggcat tcacgaaagc aacttaatcc gtcggagtca atgggttgaa
     7561 gcaactctta ttcaaaatgg ttttacgatt tcaaattctg ccttaattct gtaaaaacag
     7621 taaaattcga aggattgtaa ggtaagagtt tttttctttc tgaaaaaatg gtataatagc
     7681 aatcaaaact agaaaataaa acggaatttg gaacagattt gtctgtatcc tagtagagtg
     7741 gtgatactat gaagattagt aagaggcact tattaaatta ttccatcttg attccctact
     7801 tgcttttatc tattttgggc ttgattgtgg tctattcgac caccagtgct attttaattg
     7861 aagaaggcaa gagcgccttg cagttggttc gaaaccaagg aatcttttgg attgttagtt
     7921 tgatactgat tgccttaatt tataaattga gactagattt tttgagaaat gagcgactaa
     7981 tcattttagt tatattaata gaaatgcttt tattgttctt ggctcgtttt attggtattt
     8041 cagtaaacgg ggcatacggt tggatttcgg ttgcaggagt aactattcag ccagctgagt
     8101 acttaaaaat cattattatt tggtatttag ctcaccgatt ctccaaacag caagaagaaa
     8161 tagctactta tgattttcaa gttttgactc aaaatcaatg gcttccccgt gcttttaatg
     8221 attggcgatt cgttctccta gttctgattg gaagtttggg aattttccct gatttaggaa
     8281 atgcgactat tttagtcttg gtttccttga ttatgtatac agttagtgga atcgcttatc
     8341 gctggttttc aaccattctg gcgctcgtat ctgccacttc tgtctttgtc ttgaccacta
     8401 tcagcctaat cggtgttgag accttttcaa aaattccagt atttggctat gtagccaagc
     8461 gctttagtgc cttttttaat ccttttgccg atcgtgctga tgcaggtcac cagttagcta
     8521 attcttattt tgccatggtc aatggtggtt ggtttggtct aggtcttgga aactcgattg
     8581 aaaaacgagg ttatttgcca gaagctcata cagactttgt cttttctatc gtgattgaag
     8641 aatttggctt tgttggtgcc agtcttattt tagctctctt gtttttcatg attttgcgga
     8701 ttatcttggt cggtatccga gcggagaatc ctttcaatgc catggttgca ctcggtgtcg
     8761 gagggatgat gttggttcag gtatttgtca atatcggagg gatttcgggc ttgattccat
     8821 ctacaggagt gactttccct ttcttatccc agggtggaaa tagtcttcta gtcttatcag
     8881 tggcagtagc ctttgtctta aatattgatg ccagtgaaaa acgcgctaag ttgtaccgag
     8941 aattggaaaa tcaaccaatg aaccttctgt tgaagtagga taaagaaagg atagtttatg
     9001 tctcttcaaa aattagaaaa ttatagtaat aaaagtgttg tgcaagaaga agtcttgatt
     9061 ctaacagaat tactggaaga tattactaaa aatatgcttg ccccagagac ctttgaaaaa
     9121 ataatacagt tgaaagaatt atcaacgcag gaagattatc aaggtctaaa ccgtctagtg
     9181 actagcttat caaatgatga aatggtctat atttcacgct atttctctat cttacctctt
     9241 ttgattaata tttcagagga tgtggattta gcttatgaaa tcaatcatca aaataatatt
     9301 gatcaggact atttaggtaa attatctaca acgattaaat tggtagcaga aaaggaaaat
     9361 gccgttgaga tcctagaaca cttgaatgtt gtccctgttt tgacagccca tccaacacaa
     9421 gtgcaacgca aaagtatgtt ggatttaaca aatcatattc atagtctttt gcgtaaatac
     9481 cgtgatgtta agttggggtt gatcaataaa gataaatggt acaatgattt gcgtcgttac
     9541 atcgaaatta tcatgcagac agacatgatt cgtgagaaaa aattaaaagt gactaacgaa
     9601 atcacgaatg ctatggaata ttataacagc tcctttttga aagctgtacc tcatttgacg
     9661 acggagtata agcgcttagc gcaagcgcat ggtctgaatt taaaacaggc taaaccaatc
     9721 accatgggta tgtggatagg tggtgaccgt gatggaaatc catttgttac agcaaagacc
     9781 ttgaagcagt ctgcactcac tcagtgtgaa gtcatcatga actactatga taaaaagatt
     9841 taccaacttt atcgtgaatt ttctctttca actagcattg tcaacgtcag caagcaagtc
     9901 agagaaatgg ctcgtcaatc caaggataac tcgatttacc gcgaaaaaga gctttaccgt
     9961 cgtgccttgt ttgatattca atcaaaaatt caggcaacta aaacctatct gattgaggat
    10021 gaagaagttg ggactcgtta tgaaaccgcc aatgatttct acaaggattt gattgccatt
    10081 cgagattctc tactagaaaa taagggcgag tccttgattt caggtgattt tgtggaatta
    10141 ttgcaggcag tagagatatt tggtttttac ttagcatcaa ttgatatgcg acaagactct
    10201 agcgtctatg aagcctgtgt ggcagaactc ttgaaatcag caggaattca ttctcgttat
    10261 agcgagttga gcgaagaaga aaagtgtgac cttctcttga aagaattaga agaagatccc
    10321 cgaattcttt ctgcgactca cgcagaaaaa tcagaattat tagcaaaaga attagctatt
    10381 tttaagacgg ctcgtgtttt gaaagataag ttgggagatg atgtcatccg tcagaccatc
    10441 atttcacatg caaccagcct ttctgatatg ctagaattag ctattctgtt aaaagaagta
    10501 ggactggtgg atacggaaag ggcgcgtgtt cagattgttc ccctttttga aacaattgaa
    10561 gacttggatc attcagagga aacaatgaga aaatatcttt ctcttagcct tgccaaaaaa
    10621 tggattgact cacgaaataa ctaccaagaa atcatgcttg gctactctga cagtaataaa
    10681 gatggcggtt acttgtcatc atgttggacc ctctacaagg ctcaacaaca attgactgct
    10741 attggagatg aatttggcgt taaggttacc ttcttccatg gtcgtggtgg tactgtcggt
    10801 cgtggtggtg ggccaaccta tgaagccatt acatctcaac cgctcaagtc tatcaaggat
    10861 cgtatccgct tgacggagca gggtgaagta attgggaata aatacggtaa caaagacgcc
    10921 gcttactata accttgaaat gctagtatcg gcagctatta accgtatgat tactcagaag
    10981 aagagcgata ccaatacccc aaatcgttat gaagccatta tggatcaagt agtggaccgt
    11041 agttacgata tctaccgtga tttggtcttt ggtaatgagc atttctatga ttatttcttc
    11101 gagtcaagtc caatcaaggc tatttcaagt tttaatattg gttctcgtcc agccgctcgt
    11161 aagactatta ctgaaatcgg tggtttgcgt gccatccctt gggtattctc atggtcacag
    11221 agtcgtgtta tgttccctgg atggtacggg gttggttcaa gcttcaagga atttatcaat
    11281 aaaaatccag agaatattgc tatcttacga gatatgtacc aaaattggcc tttcttccaa
    11341 tcgcttcttt caaatgttga tatggttttg tcaaaatcaa atatgaatat tgcttttgaa
    11401 tatgctaaac tttgtgaaga cgagcaagtt aaggccatct atgagactat tttaaatgaa
    11461 tggcaagtta ctaagaacgt tatcttggct attgaaggac atgacgaact cttagctgac
    11521 aatccatatc taaaagctag tctggattac cgtatgcctt actttaatat tctcaactat
    11581 attcagttag agttgattaa acgccaacgt cgtggagaat tgtccagtga tcaagaacga
    11641 ttgattcata tcaccatcaa cggaattgcg acaggattgc gtaattcagg ttgataattt
    11701 tcaagagtga atactaaaag tgaatatcaa aaaaattcta atagactatt gacaagtaat
    11761 ttaaaaatga tataatttaa ccattcagaa aagtaatcat acaaactttt tagagagtct
    11821 gtggtagctg aaaacagata agtggcgatg atgaaaattg ggctgaatgc tatttagaat
    11881 ttgaaattat aaaaattcgg taagcacacc ttacagtgca tctcgttatt gcgagactga
    11941 gcgataggga aattccctat aattgaggtg gtaccgcgca tcgacgtcct cacacaagtt
    12001 ttttgtgtga ggattttttt gatggaggtt agtatggaaa gaaaacgatg gcgtcgcttg
    12061 tttagataag tgaaatatgt taaaggaaat aaaaaggaga aacagaatga aaaataaacg
    12121 tttaattgga attattgctg cattagcagt cttagtagca ggaagcttga tttattcttc
    12181 aatgaataaa tcagaagctc agaataataa ggatgagaag aaaataacca agattggtgt
    12241 gcttcaattt gtgagccatc catcccttga tttgatttat aaagggatcc aagatggact
    12301 tgcagaagaa ggatataaag atgatcaagt taaaattgat tttatgaact cagaaggtga
    12361 ccaaagtaag gttgcgacaa tgagtaaaca attggttgca aatgggaatg accttgtggt
    12421 tggtatcgca acaccagcag cccaagggtt ggctagtgca acaaaagact taccggttat
    12481 catggccgct attacagacc caattggtgc taacttggtt aaagatttga aaaaaccagg
    12541 tggcaacgtt acaggggtat ctgaccacaa tccagctcaa caacaagttg aactcatcaa
    12601 ggctctgaca ccgaatgtga aaacaatcgg agctctttac tcaagtagcg aagacaattc
    12661 aaaaacacag gtcgaagaat ttaaggctta tgctgaaaaa gcaggtctga cagtggaaac
    12721 atttgcagtt ccttcaacaa atgaaattgc ctcaactgtc actgttatga ctagcaaggt
    12781 agatgctatt tgggttccaa ttgataacac cattgcatca ggatttccaa cggttgtctc
    12841 tagcaatcaa agttctaaga aaccaattta tcccagtgcg acagctatgg tagaagtagg
    12901 tggtttggca tcagttgtaa ttgaccaaca tgaccttggt gtggcaacag gtaaaatgat
    12961 tgtgcaagtc ttgaaaggtg caaaaccagc cgatacccca gtcaatgtct tttcaactgg
    13021 taagtcagtc atcaataaaa aaatagcaca agaactaggt attactattc ctgagtctgt
    13081 tctcaaagaa gcaggacaag tcatcgaata aataaggtag ggaggaggag actcttccca
    13141 ttttttaaaa agatgaatat agaaaggatt aaataaaata tgattgtttc cattatttct
    13201 caaggatttg tctgggctat tctaggtctg ggaatcttta tgacatttag gattttaaac
    13261 tttccagata tgacgacaga aggttccttc cctcttgggg gagctgttgc tgtcactttg
    13321 ataaccaaag gcgtgaaccc atttttagcg acacttgttg ctgtaggagc aggttgtttg
    13381 gctggaatgg cagcaggcct tctttataca aaagggaaga tcccaacctt gctctcaggg
    13441 attttggtga tgacttcttg tcactcaatc atgctcttga ttatgggacg tgcgaattta
    13501 ggcctgcttg gaaccaagca aattcaggat gttttgcctt ttgattcgga tttgaatcaa
    13561 ctcttgacag gtctcatctt tgtgagtatt gttattgctc tcatgctctt tttcttggac
    13621 actaaactcg gacaagccta tattgctaca ggggataatc ctgatatggc tagaagtttc
    13681 gggattcata ctggacgcat ggagctcatg ggcttggtct tatcaaatgg tgtgattgcc
    13741 cttgcaggtg ccctcattgc tcagcaagaa ggctatgccg atgtgtctcg agggatcggg
    13801 gttatcgttg tggggcttgc aagtttgatt attggagaag ttattttcaa gagtttgagc
    13861 ttggcagagc gtttggttac tatcgttgta ggttctatcg cttatcaatt tttagtgtgg
    13921 gcagttatcg cacttggctt taatacaagt taccttcgtt tatacagtgc cttgatttta
    13981 gcagtctgcc tcatgattcc aacatttaag caaacaatct tgaaaggagc caagttaagc
    14041 aaatgacagc aattgtagaa ttaaaaaatg caaccaaaat cgttaaaaat ggctttgatg
    14101 aagaaaagat tattttaaat gatgtttcct tagaaatttt tgaacgggac tttatcacga
    14161 ttttgggcgg aaatggtgct ggaaaatcaa ctctctttaa cactatagca gggaccttat
    14221 cactaactag tggaactatc cgtattttag gtgaagatct cactaagttt tcacccgaga
    14281 agcgtgccaa gtacctgtct cgtgtcttcc aagatccaaa gatggggaca gctccccgta
    14341 tgacggtcgc tgaaaatctt ttaatcgcca agtttcgtgg tgaaaagcgt ggattgttac
    14401 caagacgctt gactagctat aaggatgaat ttcaggcaac cattgaaaaa gtaggaaatg
    14461 gtcttgagaa acacttgaat acaccgattg agttcttatc aggtggacaa agacaggctt
    14521 tgagtctctt gatggcaacc ttgaagcgac ctgaattact cctgttagat gagcatactg
    14581 ctgccctgga tccaaagact agtgttgctt tgatggaatt gacagatgaa tttgttaaga
    14641 aagatcagct aacagccctt atgattactc atcatatgga agatgctctc aaatacggca
    14701 atcgcttgat tgtcatgaaa gaaggacgga ttatccaaga tttaaaccaa gaagaaaaag
    14761 caaaaatgaa aatctctgat tattatcaac tctttgaata aagtagggaa aatgagtgaa
    14821 atggtttact ttttttctga aataaagtat actatataaa gtaaactatg ataacatgga
    14881 ggt
//
